Prkch cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Prkch
Entrez Gene ID	18755
Full Name	protein kinase C, eta
Synonyms	Pkch
General protein information	Preferred Names protein kinase C, eta Names protein kinase C eta type PKC-L nPKC-eta
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 12 Map Location: 12\|12 C3
Summary	Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. It is a calcium-independent and phospholipids-dependent protein kinase. It is predominantly expressed in epithelial tissues and has been shown to reside specifically in the cell nucleus. This protein kinase can regulate keratinocyte differentiation by activating the MAP kinase MAPK13 (p38delta)-activated protein kinase cascade that targets CCAAT/enhancer-binding protein alpha (CEBPA). It is also found to mediate the transcription activation of the transglutaminase 1 (TGM1) gene. Mutations in the human gene are associated with susceptibility to cerebral infarction. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2015].

mRNA and Protein(s)

mRNA	Protein	Name
NM_008856.4	NP_032882.2	protein kinase C eta type isoform 1
NM_001313977.1	NP_001300906.1	protein kinase C eta type isoform 2

Pathways from BioSystems

Homologies

	PRKCH	XP_002805131.1
Rattus norvegicus (Norway rat)	Prkch	NP_112347.1
Xenopus tropicalis (tropical clawed frog)	prkch	XP_002939521.2
Canis lupus familiaris (dog)	PRKCH	XP_547844.2
Gallus gallus (chicken)	PRKCH	XP_421417.3
Danio rerio (zebrafish)	prkcha	NP_001116528.1
Homo sapiens (human)	PRKCH	NP_006246.2
Bos taurus (cattle)	PRKCH	NP_001070331.1
Mus musculus (house mouse)	Prkch	NP_032882.2
Pan troglodytes (chimpanzee)	PRKCH	XP_001168420.1

Gene Ontology
Bibliography

Related articles in PubMed

Protein kinase C-? controls CTLA-4-mediated regulatory T cell function.
Kong KF, Fu G, Zhang Y, Yokosuka T, Casas J, Canonigo-Balancio AJ, Becart S, Kim G, Yates JR 3rd, Kronenberg M, Saito T, Gascoigne NR, Altman A
Nature immunology15(5)465-72(2014 May)

Costimulatory molecule DNAM-1 is essential for optimal differentiation of memory natural killer cells during mouse cytomegalovirus infection.
Nabekura T, Kanaya M, Shibuya A, Fu G, Gascoigne NR, Lanier LL
Immunity40(2)225-34(2014 Feb)

The lymphoid lineage-specific actin-uncapping protein Rltpr is essential for costimulation via CD28 and the development of regulatory T cells.
Liang Y, Cucchetti M, Roncagalli R, Yokosuka T, Malzac A, Bertosio E, Imbert J, Nijman IJ, Suchanek M, Saito T, W?lfing C, Malissen B, Malissen M
Nature immunology14(8)858-66(2013 Aug)

Protein kinase C ? is required for T cell activation and homeostatic proliferation.
Fu G, Hu J, Niederberger-Magnenat N, Rybakin V, Casas J, Yachi PP, Feldstein S, Ma B, Hoerter JA, Ampudia J, Rigaud S, Lambolez F, Gavin AL, Sauer K, Cheroutre H, Gascoigne NR
Science signaling4(202)ra84(2011 Dec)

A cascade of protein kinase C isozymes promotes cytoskeletal polarization in T cells.
Quann EJ, Liu X, Altan-Bonnet G, Huse M
Nature immunology12(7)647-54(2011 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

genetic deficiency results in defective suppressive activity of T reg cells
Title: Protein kinase C-? controls CTLA-4-mediated regulatory T cell function.

data suggest that PKCeta shares some redundant roles with PKCtheta; in T cell biology and also performs nonredundant functions that are required for T cell homeostasis and activation
Title: Protein kinase C ? is required for T cell activation and homeostatic proliferation.

Protein kinase C eta promotes a proliferation to differentiation switch in keratinocytes via upregulation of p27Kip1 mRNA through suppression of JNK/c-Jun signaling under stress conditions.
Title: PKC? promotes a proliferation to differentiation switch in keratinocytes via upregulation of p27Kip1 mRNA through suppression of JNK/c-Jun signaling under stress conditions.

these results establish a previously uncharacterized role forPKC-epsilon, PKC-eta, PKC-theta in T cell polarity.
Title: A cascade of protein kinase C isozymes promotes cytoskeletal polarization in T cells.

ATP1A1 interaction with PKCeta and occludin was involved in the integrity of the blood-labyrinth-barrier
Title: Na+/K+-ATPase ?1 identified as an abundant protein in the blood-labyrinth barrier that plays an essential role in the barrier integrity.

The following Prkch gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Prkch cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu15819	NM_008856.4 Latest version!	Mus musculus protein kinase C, eta (Prkch), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu83515	NM_001313977.1 Latest version!	Mus musculus protein kinase C, eta (Prkch), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Prkch ( NM_008856.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_032882.2 Mus musculus protein kinase C, eta (Prkch), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu15819
Clone ID Related Accession (Same CDS sequence)	NM_008856.4
Accession Version	NM_008856.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2052bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-24
Organism	Mus musculus(house mouse)
Product	protein kinase C eta type isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC124179.4, AK145817.1, AK164044.1 and BF462787.1. On Sep 18, 2015 this sequence version replaced NM_008856.3. Transcript Variant: This variant (1) encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC031121.1, AK145817.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCGTCCG GCACGATGAA GTTCAATGGC TATCTGAGGG TCCGCATCGG AGAGGCTGTA 
GGGCTGCAGC CCACCCGCTG GTCCCTGCGG CACTCGCTCT TCAAAAAGGG CCACCAGCTG 
CTGGACCCCT ACCTGACGGT GAGCGTAGAC CAGGTACGCG TGGGCCAGAC CAGCACAAAG 
CAGAAGACCA ACAAACCCAC CTACAACGAG GAGTTCTGCG CCAATGTCAC CGACGGCGGC 
CACCTGGAGC TAGCCGTCTT CCACGAGACG CCCCTGGGTT ATGACCACTT TGTGGCCAAC 
TGCACGCTGC AGTTCCAGGA GCTGTTGCGC ACGGCTGGTA CCTCGGACAC CTTCGAGGGC 
TGGGTGGATC TGGAGCCTGA GGGGAAAGTG TTTGTGGTAA TAACCCTAAC AGGGAGTTTC 
ACTGAAGCCA CTCTCCAGAG AGACCGCATC TTCAAGCATT TTACCAGGAA GCGCCAAAGG 
GCTATGCGAA GACGAGTCCA TCAAGTGAAC GGACATAAGT TCATGGCCAC GTACCTGAGG 
CAGCCCACCT ACTGCTCTCA TTGCCGAGAG TTCATCTGGG GAGTATTTGG GAAACAGGGT 
TATCAATGCC AAGTGTGCAC CTGCGTCGTC CATAAACGCT GCCATCATCT AATTGTTACA 
GCCTGCACTT GCCAAAACAA TATTAACAAA GTGGATGCCA AGATTGCAGA ACAGCGGTTT 
GGCATCAACA TCCCACACAA GTTCAACGTT CACAATTACA AGGTGCCCAC GTTCTGTGAC 
CACTGTGGCT CCCTGCTCTG GGGGATAATG CGACAAGGAC TTCAGTGTAA AATATGTAAG 
ATGAATGTAC ATATTCGGTG TCAGGCGAAC GTGGCCCCAA ACTGCGGGGT GAATGCCGTG 
GAGCTTGCCA AGACCCTGGC AGGGATGGGT CTCCAACCCG GAAATATTTC TCCAACCTCG 
AAACTCATTT CCAGGTCGAC ACTAAGACGG CAGGGAAAGG AGGGCTCCAA AGAAGGAAAT 
GGGATCGGTG TTAACTCTTC CAGCAGATTC GGCATCGACA ACTTTGAGTT CATCCGGGTG 
TTGGGGAAGG GGAGCTTCGG GAAGGTGATG CTTGCCAGGA TAAAGGAGAC AGGAGAACTG 
TACGCCGTGA AGGTGCTGAA GAAGGACGTG ATTCTGCAGG ATGATGATGT AGAGTGCACC 
ATGACTGAGA AGAGGATCCT GTCCTTGGCC CGCAACCACC CCTTCCTCAC CCAGCTCTTC 
TGCTGCTTTC AGACTCCAGA CCGTCTGTTC TTTGTCATGG AGTTTGTGAA CGGAGGCGAC 
CTGATGTTCC ACATCCAAAA GTCCCGTCGT TTCGATGAAG CCCGTGCTCG TTTCTACGCC 
GCGGAGATCA TTTCTGCACT CATGTTCCTA CATGAGAAAG GTATCATCTA TAGAGACTTG 
AAACTGGACA ATGTGCTATT GGACCACGAA GGTCACTGTA AACTGGCCGA CTTTGGAATG 
TGCAAGGAGG GGATTTGTAA TGGGGTCACC ACAGCCACCT TCTGCGGTAC ACCCGACTAC 
ATTGCCCCGG AGATCCTTCA GGAGATGCTG TATGGACCTG CAGTAGACTG GTGGGCCATG 
GGTGTGTTGC TTTATGAGAT GCTGTGCGGA CATGCGCCCT TTGAGGCTGA AAATGAAGAT 
GACCTTTTTG AGGCCATACT GAATGATGAA GTCGTCTACC CCACCTGGCT CCACGAAGAT 
GCCACAGGGA TCCTCAAGTC TTTCATGACC AAGAACCCCA CCATGCGCTT GGGCAGCCTG 
ACTCAGGGAG GAGAGCATGA GATCCTGAGA CACCCTTTCT TTAAGGAAAT CGACTGGGCC 
CAGTTGAACC ATCGCCAGTT AGAGCCGCCT TTCCGACCTA GAATCAAATC CCGAGAAGAT 
GTCAGCAATT TTGACCCAGA CTTTATAAAA GAAGAGCCCG TCTTAACTCC GATTGATGAG 
GGACATCTTC CTATGATTAA CCAGGATGAG TTTAGAAACT TTTCCTATGT GTCACCGGAA 
TTGCAACTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_032882.2
CDS	368..2419
Translation	MSSGTMKFNGYLRVRIGEAVGLQPTRWSLRHSLFKKGHQLLDPYLTVSVDQVRVGQTSTKQKTNKPTYNEEFCANVTDGGHLELAVFHETPLGYDHFVANCTLQFQELLRTAGTSDTFEGWVDLEPEGKVFVVITLTGSFTEATLQRDRIFKHFTRKRQRAMRRRVHQVNGHKFMATYLRQPTYCSHCREFIWGVFGKQGYQCQVCTCVVHKRCHHLIVTACTCQNNINKVDAKIAEQRFGINIPHKFNVHNYKVPTFCDHCGSLLWGIMRQGLQCKICKMNVHIRCQANVAPNCGVNAVELAKTLAGMGLQPGNISPTSKLISRSTLRRQGKEGSKEGNGIGVNSSSRFGIDNFEFIRVLGKGSFGKVMLARIKETGELYAVKVLKKDVILQDDDVECTMTEKRILSLARNHPFLTQLFCCFQTPDRLFFVMEFVNGGDLMFHIQKSRRFDEARARFYAAEIISALMFLHEKGIIYRDLKLDNVLLDHEGHCKLADFGMCKEGICNGVTTATFCGTPDYIAPEILQEMLYGPAVDWWAMGVLLYEMLCGHAPFEAENEDDLFEAILNDEVVYPTWLHEDATGILKSFMTKNPTMRLGSLTQGGEHEILRHPFFKEIDWAQLNHRQLEPPFRPRIKSREDVSNFDPDFIKEEPVLTPIDEGHLPMINQDEFRNFSYVSPELQL

Target ORF information:

RefSeq Version	NM_008856.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus protein kinase C, eta (Prkch), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_008856.4

ORF Insert Sequence:

ATGTCGTCCG GCACGATGAA GTTCAATGGC TATCTGAGGG TCCGCATCGG AGAGGCTGTA 
GGGCTGCAGC CCACCCGCTG GTCCCTGCGG CACTCGCTCT TCAAAAAGGG CCACCAGCTG 
CTGGACCCCT ACCTGACGGT GAGCGTAGAC CAGGTACGCG TGGGCCAGAC CAGCACAAAG 
CAGAAGACCA ACAAACCCAC CTACAACGAG GAGTTCTGCG CCAATGTCAC CGACGGCGGC 
CACCTGGAGC TAGCCGTCTT CCACGAGACG CCCCTGGGTT ATGACCACTT TGTGGCCAAC 
TGCACGCTGC AGTTCCAGGA GCTGTTGCGC ACGGCTGGTA CCTCGGACAC CTTCGAGGGC 
TGGGTGGATC TGGAGCCTGA GGGGAAAGTG TTTGTGGTAA TAACCCTAAC AGGGAGTTTC 
ACTGAAGCCA CTCTCCAGAG AGACCGCATC TTCAAGCATT TTACCAGGAA GCGCCAAAGG 
GCTATGCGAA GACGAGTCCA TCAAGTGAAC GGACATAAGT TCATGGCCAC GTACCTGAGG 
CAGCCCACCT ACTGCTCTCA TTGCCGAGAG TTCATCTGGG GAGTATTTGG GAAACAGGGT 
TATCAATGCC AAGTGTGCAC CTGCGTCGTC CATAAACGCT GCCATCATCT AATTGTTACA 
GCCTGCACTT GCCAAAACAA TATTAACAAA GTGGATGCCA AGATTGCAGA ACAGCGGTTT 
GGCATCAACA TCCCACACAA GTTCAACGTT CACAATTACA AGGTGCCCAC GTTCTGTGAC 
CACTGTGGCT CCCTGCTCTG GGGGATAATG CGACAAGGAC TTCAGTGTAA AATATGTAAG 
ATGAATGTAC ATATTCGGTG TCAGGCGAAC GTGGCCCCAA ACTGCGGGGT GAATGCCGTG 
GAGCTTGCCA AGACCCTGGC AGGGATGGGT CTCCAACCCG GAAATATTTC TCCAACCTCG 
AAACTCATTT CCAGGTCGAC ACTAAGACGG CAGGGAAAGG AGGGCTCCAA AGAAGGAAAT 
GGGATCGGTG TTAACTCTTC CAGCAGATTC GGCATCGACA ACTTTGAGTT CATCCGGGTG 
TTGGGGAAGG GGAGCTTCGG GAAGGTGATG CTTGCCAGGA TAAAGGAGAC AGGAGAACTG 
TACGCCGTGA AGGTGCTGAA GAAGGACGTG ATTCTGCAGG ATGATGATGT AGAGTGCACC 
ATGACTGAGA AGAGGATCCT GTCCTTGGCC CGCAACCACC CCTTCCTCAC CCAGCTCTTC 
TGCTGCTTTC AGACTCCAGA CCGTCTGTTC TTTGTCATGG AGTTTGTGAA CGGAGGCGAC 
CTGATGTTCC ACATCCAAAA GTCCCGTCGT TTCGATGAAG CCCGTGCTCG TTTCTACGCC 
GCGGAGATCA TTTCTGCACT CATGTTCCTA CATGAGAAAG GTATCATCTA TAGAGACTTG 
AAACTGGACA ATGTGCTATT GGACCACGAA GGTCACTGTA AACTGGCCGA CTTTGGAATG 
TGCAAGGAGG GGATTTGTAA TGGGGTCACC ACAGCCACCT TCTGCGGTAC ACCCGACTAC 
ATTGCCCCGG AGATCCTTCA GGAGATGCTG TATGGACCTG CAGTAGACTG GTGGGCCATG 
GGTGTGTTGC TTTATGAGAT GCTGTGCGGA CATGCGCCCT TTGAGGCTGA AAATGAAGAT 
GACCTTTTTG AGGCCATACT GAATGATGAA GTCGTCTACC CCACCTGGCT CCACGAAGAT 
GCCACAGGGA TCCTCAAGTC TTTCATGACC AAGAACCCCA CCATGCGCTT GGGCAGCCTG 
ACTCAGGGAG GAGAGCATGA GATCCTGAGA CACCCTTTCT TTAAGGAAAT CGACTGGGCC 
CAGTTGAACC ATCGCCAGTT AGAGCCGCCT TTCCGACCTA GAATCAAATC CCGAGAAGAT 
GTCAGCAATT TTGACCCAGA CTTTATAAAA GAAGAGCCCG TCTTAACTCC GATTGATGAG 
GGACATCTTC CTATGATTAA CCAGGATGAG TTTAGAAACT TTTCCTATGT GTCACCGGAA 
TTGCAACTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Prkch ( NM_001313977.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001300906.1 Mus musculus protein kinase C, eta (Prkch), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu83515
Clone ID Related Accession (Same CDS sequence)	NM_001313977.1
Accession Version	NM_001313977.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1713bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-24
Organism	Mus musculus(house mouse)
Product	protein kinase C eta type isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC124179.4, AK164044.1 and BF462787.1. Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (2) has a distinct N-terminus and is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK155951.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849390 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGGCCG GTCGTCAGGT ATTGGTGGAT CTGGAGCCTG AGGGGAAAGT GTTTGTGGTA 
ATAACCCTAA CAGGGAGTTT CACTGAAGCC ACTCTCCAGA GAGACCGCAT CTTCAAGCAT 
TTTACCAGGA AGCGCCAAAG GGCTATGCGA AGACGAGTCC ATCAAGTGAA CGGACATAAG 
TTCATGGCCA CGTACCTGAG GCAGCCCACC TACTGCTCTC ATTGCCGAGA GTTCATCTGG 
GGAGTATTTG GGAAACAGGG TTATCAATGC CAAGTGTGCA CCTGCGTCGT CCATAAACGC 
TGCCATCATC TAATTGTTAC AGCCTGCACT TGCCAAAACA ATATTAACAA AGTGGATGCC 
AAGATTGCAG AACAGCGGTT TGGCATCAAC ATCCCACACA AGTTCAACGT TCACAATTAC 
AAGGTGCCCA CGTTCTGTGA CCACTGTGGC TCCCTGCTCT GGGGGATAAT GCGACAAGGA 
CTTCAGTGTA AAATATGTAA GATGAATGTA CATATTCGGT GTCAGGCGAA CGTGGCCCCA 
AACTGCGGGG TGAATGCCGT GGAGCTTGCC AAGACCCTGG CAGGGATGGG TCTCCAACCC 
GGAAATATTT CTCCAACCTC GAAACTCATT TCCAGGTCGA CACTAAGACG GCAGGGAAAG 
GAGGGCTCCA AAGAAGGAAA TGGGATCGGT GTTAACTCTT CCAGCAGATT CGGCATCGAC 
AACTTTGAGT TCATCCGGGT GTTGGGGAAG GGGAGCTTCG GGAAGGTGAT GCTTGCCAGG 
ATAAAGGAGA CAGGAGAACT GTACGCCGTG AAGGTGCTGA AGAAGGACGT GATTCTGCAG 
GATGATGATG TAGAGTGCAC CATGACTGAG AAGAGGATCC TGTCCTTGGC CCGCAACCAC 
CCCTTCCTCA CCCAGCTCTT CTGCTGCTTT CAGACTCCAG ACCGTCTGTT CTTTGTCATG 
GAGTTTGTGA ACGGAGGCGA CCTGATGTTC CACATCCAAA AGTCCCGTCG TTTCGATGAA 
GCCCGTGCTC GTTTCTACGC CGCGGAGATC ATTTCTGCAC TCATGTTCCT ACATGAGAAA 
GGTATCATCT ATAGAGACTT GAAACTGGAC AATGTGCTAT TGGACCACGA AGGTCACTGT 
AAACTGGCCG ACTTTGGAAT GTGCAAGGAG GGGATTTGTA ATGGGGTCAC CACAGCCACC 
TTCTGCGGTA CACCCGACTA CATTGCCCCG GAGATCCTTC AGGAGATGCT GTATGGACCT 
GCAGTAGACT GGTGGGCCAT GGGTGTGTTG CTTTATGAGA TGCTGTGCGG ACATGCGCCC 
TTTGAGGCTG AAAATGAAGA TGACCTTTTT GAGGCCATAC TGAATGATGA AGTCGTCTAC 
CCCACCTGGC TCCACGAAGA TGCCACAGGG ATCCTCAAGT CTTTCATGAC CAAGAACCCC 
ACCATGCGCT TGGGCAGCCT GACTCAGGGA GGAGAGCATG AGATCCTGAG ACACCCTTTC 
TTTAAGGAAA TCGACTGGGC CCAGTTGAAC CATCGCCAGT TAGAGCCGCC TTTCCGACCT 
AGAATCAAAT CCCGAGAAGA TGTCAGCAAT TTTGACCCAG ACTTTATAAA AGAAGAGCCC 
GTCTTAACTC CGATTGATGA GGGACATCTT CCTATGATTA ACCAGGATGA GTTTAGAAAC 
TTTTCCTATG TGTCACCGGA ATTGCAACTG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001300906.1
CDS	804..2516
Translation	MEAGRQVLVDLEPEGKVFVVITLTGSFTEATLQRDRIFKHFTRKRQRAMRRRVHQVNGHKFMATYLRQPTYCSHCREFIWGVFGKQGYQCQVCTCVVHKRCHHLIVTACTCQNNINKVDAKIAEQRFGINIPHKFNVHNYKVPTFCDHCGSLLWGIMRQGLQCKICKMNVHIRCQANVAPNCGVNAVELAKTLAGMGLQPGNISPTSKLISRSTLRRQGKEGSKEGNGIGVNSSSRFGIDNFEFIRVLGKGSFGKVMLARIKETGELYAVKVLKKDVILQDDDVECTMTEKRILSLARNHPFLTQLFCCFQTPDRLFFVMEFVNGGDLMFHIQKSRRFDEARARFYAAEIISALMFLHEKGIIYRDLKLDNVLLDHEGHCKLADFGMCKEGICNGVTTATFCGTPDYIAPEILQEMLYGPAVDWWAMGVLLYEMLCGHAPFEAENEDDLFEAILNDEVVYPTWLHEDATGILKSFMTKNPTMRLGSLTQGGEHEILRHPFFKEIDWAQLNHRQLEPPFRPRIKSREDVSNFDPDFIKEEPVLTPIDEGHLPMINQDEFRNFSYVSPELQL

Target ORF information:

RefSeq Version	NM_001313977.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus protein kinase C, eta (Prkch), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001313977.1

ORF Insert Sequence:

ATGGAGGCCG GTCGTCAGGT ATTGGTGGAT CTGGAGCCTG AGGGGAAAGT GTTTGTGGTA 
ATAACCCTAA CAGGGAGTTT CACTGAAGCC ACTCTCCAGA GAGACCGCAT CTTCAAGCAT 
TTTACCAGGA AGCGCCAAAG GGCTATGCGA AGACGAGTCC ATCAAGTGAA CGGACATAAG 
TTCATGGCCA CGTACCTGAG GCAGCCCACC TACTGCTCTC ATTGCCGAGA GTTCATCTGG 
GGAGTATTTG GGAAACAGGG TTATCAATGC CAAGTGTGCA CCTGCGTCGT CCATAAACGC 
TGCCATCATC TAATTGTTAC AGCCTGCACT TGCCAAAACA ATATTAACAA AGTGGATGCC 
AAGATTGCAG AACAGCGGTT TGGCATCAAC ATCCCACACA AGTTCAACGT TCACAATTAC 
AAGGTGCCCA CGTTCTGTGA CCACTGTGGC TCCCTGCTCT GGGGGATAAT GCGACAAGGA 
CTTCAGTGTA AAATATGTAA GATGAATGTA CATATTCGGT GTCAGGCGAA CGTGGCCCCA 
AACTGCGGGG TGAATGCCGT GGAGCTTGCC AAGACCCTGG CAGGGATGGG TCTCCAACCC 
GGAAATATTT CTCCAACCTC GAAACTCATT TCCAGGTCGA CACTAAGACG GCAGGGAAAG 
GAGGGCTCCA AAGAAGGAAA TGGGATCGGT GTTAACTCTT CCAGCAGATT CGGCATCGAC 
AACTTTGAGT TCATCCGGGT GTTGGGGAAG GGGAGCTTCG GGAAGGTGAT GCTTGCCAGG 
ATAAAGGAGA CAGGAGAACT GTACGCCGTG AAGGTGCTGA AGAAGGACGT GATTCTGCAG 
GATGATGATG TAGAGTGCAC CATGACTGAG AAGAGGATCC TGTCCTTGGC CCGCAACCAC 
CCCTTCCTCA CCCAGCTCTT CTGCTGCTTT CAGACTCCAG ACCGTCTGTT CTTTGTCATG 
GAGTTTGTGA ACGGAGGCGA CCTGATGTTC CACATCCAAA AGTCCCGTCG TTTCGATGAA 
GCCCGTGCTC GTTTCTACGC CGCGGAGATC ATTTCTGCAC TCATGTTCCT ACATGAGAAA 
GGTATCATCT ATAGAGACTT GAAACTGGAC AATGTGCTAT TGGACCACGA AGGTCACTGT 
AAACTGGCCG ACTTTGGAAT GTGCAAGGAG GGGATTTGTA ATGGGGTCAC CACAGCCACC 
TTCTGCGGTA CACCCGACTA CATTGCCCCG GAGATCCTTC AGGAGATGCT GTATGGACCT 
GCAGTAGACT GGTGGGCCAT GGGTGTGTTG CTTTATGAGA TGCTGTGCGG ACATGCGCCC 
TTTGAGGCTG AAAATGAAGA TGACCTTTTT GAGGCCATAC TGAATGATGA AGTCGTCTAC 
CCCACCTGGC TCCACGAAGA TGCCACAGGG ATCCTCAAGT CTTTCATGAC CAAGAACCCC 
ACCATGCGCT TGGGCAGCCT GACTCAGGGA GGAGAGCATG AGATCCTGAG ACACCCTTTC 
TTTAAGGAAA TCGACTGGGC CCAGTTGAAC CATCGCCAGT TAGAGCCGCC TTTCCGACCT 
AGAATCAAAT CCCGAGAAGA TGTCAGCAAT TTTGACCCAG ACTTTATAAA AGAAGAGCCC 
GTCTTAACTC CGATTGATGA GGGACATCTT CCTATGATTA ACCAGGATGA GTTTAGAAAC 
TTTTCCTATG TGTCACCGGA ATTGCAACTG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Protein kinase C-? controls CTLA-4-mediated regulatory T cell function.
Nature immunology15(5)465-72(2014 May)
Kong KF,Fu G,Zhang Y,Yokosuka T,Casas J,Canonigo-Balancio AJ,Becart S,Kim G,Yates JR 3rd,Kronenberg M,Saito T,Gascoigne NR,Altman A

Costimulatory molecule DNAM-1 is essential for optimal differentiation of memory natural killer cells during mouse cytomegalovirus infection.
Immunity40(2)225-34(2014 Feb)
Nabekura T,Kanaya M,Shibuya A,Fu G,Gascoigne NR,Lanier LL

The lymphoid lineage-specific actin-uncapping protein Rltpr is essential for costimulation via CD28 and the development of regulatory T cells.
Nature immunology14(8)858-66(2013 Aug)
Liang Y,Cucchetti M,Roncagalli R,Yokosuka T,Malzac A,Bertosio E,Imbert J,Nijman IJ,Suchanek M,Saito T,W?lfing C,Malissen B,Malissen M

Protein kinase C ? is required for T cell activation and homeostatic proliferation.
Science signaling4(202)ra84(2011 Dec)
Fu G,Hu J,Niederberger-Magnenat N,Rybakin V,Casas J,Yachi PP,Feldstein S,Ma B,Hoerter JA,Ampudia J,Rigaud S,Lambolez F,Gavin AL,Sauer K,Cheroutre H,Gascoigne NR

A cascade of protein kinase C isozymes promotes cytoskeletal polarization in T cells.
Nature immunology12(7)647-54(2011 May)
Quann EJ,Liu X,Altan-Bonnet G,Huse M

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Prkch cDNA ORF clone, Mus musculus(house mouse)

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