mink1 gene cDNA ORF clone, Danio rerio(zebrafish)

mRNA	Protein	Name
XM_017356005.2	XP_017211494.1	misshapen-like kinase 1 isoform X4
XM_017356005.1	XP_017211494.1	misshapen-like kinase 1 isoform X1
NM_001313841.1	NP_001300770.1	misshapen-like kinase 1 isoform 1
NM_001089579.2	NP_001083048.2	misshapen-like kinase 1 isoform 2
XM_021475971.1	XP_021331646.1	misshapen-like kinase 1 isoform X2
XM_017356008.2	XP_017211497.1	misshapen-like kinase 1 isoform X7
XM_017356009.1	XP_017211498.1	misshapen-like kinase 1 isoform X5
XM_021475972.1	XP_021331647.1	misshapen-like kinase 1 isoform X3
XM_021475973.1	XP_021331648.1	misshapen-like kinase 1 isoform X6
XM_017356006.1	XP_017211495.1	misshapen-like kinase 1 isoform X2
XM_017356007.1	XP_017211496.1	misshapen-like kinase 1 isoform X3
XM_017356008.1	XP_017211497.1	misshapen-like kinase 1 isoform X4
XM_017356010.1	XP_017211499.1	misshapen-like kinase 1 isoform X6
XM_017356006.2	XP_017211495.1	misshapen-like kinase 1 isoform X5
XM_021475970.1	XP_021331645.1	misshapen-like kinase 1 isoform X1
XM_017356010.2	XP_017211499.1	misshapen-like kinase 1 isoform X8

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa53202	NM_001089579.2 Latest version!	Danio rerio misshapen-like kinase 1 (mink1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
ODa60471	NM_001313841.1 Latest version!	Danio rerio misshapen-like kinase 1 (mink1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
ODa142547	XM_021475970.1 Latest version!	Danio rerio misshapen-like kinase 1 (mink1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
ODa142548	XM_021475971.1 Latest version!	Danio rerio misshapen-like kinase 1 (mink1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
ODa142549	XM_021475972.1 Latest version!	Danio rerio misshapen-like kinase 1 (mink1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
ODa142550	XM_021475973.1 Latest version!	Danio rerio misshapen-like kinase 1 (mink1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
ODa59033	XM_017356005.2 Latest version!	Danio rerio misshapen-like kinase 1 (mink1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
ODa59034	XM_017356006.2 Latest version!	Danio rerio misshapen-like kinase 1 (mink1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
ODa59035	XM_017356007.1 Latest version!	Danio rerio misshapen-like kinase 1 (mink1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
ODa59036	XM_017356008.2 Latest version!	Danio rerio misshapen-like kinase 1 (mink1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
ODa59037	XM_017356009.1 Latest version!	Danio rerio misshapen-like kinase 1 (mink1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
ODa59038	XM_017356010.2 Latest version!	Danio rerio misshapen-like kinase 1 (mink1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
View Old Accession Versions >>
ODa59033	XM_017356005.1	Danio rerio misshapen-like kinase 1 (mink1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
ODa59036	XM_017356008.1	Danio rerio misshapen-like kinase 1 (mink1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
ODa59034	XM_017356006.1	Danio rerio misshapen-like kinase 1 (mink1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
ODa59038	XM_017356010.1	Danio rerio misshapen-like kinase 1 (mink1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
Hide Old Accession Versions >>

mink1 ( NM_001089579.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001083048.2 Danio rerio misshapen-like kinase 1 (mink1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	ODa53202
Clone ID Related Accession (Same CDS sequence)	NM_001089579.2
Accession Version	NM_001089579.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 471bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-26
Organism	Danio rerio(zebrafish)
Product	misshapen-like kinase 1 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX324194.7. On Sep 4, 2015 this sequence version replaced NM_001089579.1. Transcript Variant: This variant (2) lacks several exons and its 3' terminal exon extends past a splice site that is used in variant 1. This results in a novel 3' coding region and 3' UTR, compared to variant 1. It encodes isoform 2 which is shorter and has a distinct C-terminus, compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: BC133893.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCAGAAA ACGCACCTAC GCGGAGTCTG GATGACATCG ACCTGGCCGC CCTGCGGGAC 
CCAGCAGGGA TCTTCGAGTT GGTGGAGGTC GTGGGCAACG GCACCTATGG ACAAGTTTAC 
AAGGGTCGTC ATGTTAAGAC AGGTCAGCTG GCAGCCATAA AAGTCATGGA TGTGACTGAG 
GAAGAAGAGG AGGAGATTAA AGCGGAGATT AACATGCTGA AGAAGTACAG TCACCATAGA 
AATATCGCCA CCTACTATGG AGCCTTTGTG AAGAAGAGTC CTCCTGGACA CGATGACCAG 
CTCTGGTTGG TGATGGAGTT TTGTGGTGCC GGCTCAGTAA CAGACTTGGT GAAGAACACA 
AAAGGCAACT CTTTAAAAGA AGACTGGATC GCCTATATCT GCAGAGAGAT CCTTAGGGTG 
AGACTCTTTA AGTCTCTTTT TATTGCAGTG ACTGTTATTA TGCAGTGGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001083048.2
CDS	467..937
Translation	MSENAPTRSLDDIDLAALRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEEEEEEIKAEINMLKKYSHHRNIATYYGAFVKKSPPGHDDQLWLVMEFCGAGSVTDLVKNTKGNSLKEDWIAYICREILRVRLFKSLFIAVTVIMQW

Target ORF information:

RefSeq Version	NM_001089579.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio misshapen-like kinase 1 (mink1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001089579.2

ORF Insert Sequence:

ATGTCAGAAA ACGCACCTAC GCGGAGTCTG GATGACATCG ACCTGGCCGC CCTGCGGGAC 
CCAGCAGGGA TCTTCGAGTT GGTGGAGGTC GTGGGCAACG GCACCTATGG ACAAGTTTAC 
AAGGGTCGTC ATGTTAAGAC AGGTCAGCTG GCAGCCATAA AAGTCATGGA TGTGACTGAG 
GAAGAAGAGG AGGAGATTAA AGCGGAGATT AACATGCTGA AGAAGTACAG TCACCATAGA 
AATATCGCCA CCTACTATGG AGCCTTTGTG AAGAAGAGTC CTCCTGGACA CGATGACCAG 
CTCTGGTTGG TGATGGAGTT TTGTGGTGCC GGCTCAGTAA CAGACTTGGT GAAGAACACA 
AAAGGCAACT CTTTAAAAGA AGACTGGATC GCCTATATCT GCAGAGAGAT CCTTAGGGTG 
AGACTCTTTA AGTCTCTTTT TATTGCAGTG ACTGTTATTA TGCAGTGGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mink1 ( NM_001313841.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001300770.1 Danio rerio misshapen-like kinase 1 (mink1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	ODa60471
Clone ID Related Accession (Same CDS sequence)	NM_001313841.1
Accession Version	NM_001313841.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3822bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-26
Organism	Danio rerio(zebrafish)
Product	misshapen-like kinase 1 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX324194.7. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTCAGAAA ACGCACCTAC GCGGAGTCTG GATGACATCG ACCTGGCCGC CCTGCGGGAC 
CCAGCAGGGA TCTTCGAGTT GGTGGAGGTC GTGGGCAACG GCACCTATGG ACAAGTTTAC 
AAGGGTCGTC ATGTTAAGAC AGGTCAGCTG GCAGCCATAA AAGTCATGGA TGTGACTGAG 
GAAGAAGAGG AGGAGATTAA AGCGGAGATT AACATGCTGA AGAAGTACAG TCACCATAGA 
AATATCGCCA CCTACTATGG AGCCTTTGTG AAGAAGAGTC CTCCTGGACA CGATGACCAG 
CTCTGGTTGG TGATGGAGTT TTGTGGTGCC GGCTCAGTAA CAGACTTGGT GAAGAACACA 
AAAGGCAACT CTTTAAAAGA AGACTGGATC GCCTATATCT GCAGAGAGAT CCTTAGGGGT 
CTGTCTCACC TCCATGCTCA TAAAGTCATC CACCGAGACA TCAAGGGACA GAATGTGCTG 
CTTACAGAGA ACGCAGAGGT TAAACTGGTG GATTTTGGGG TGAGCGCACA GTTGGATCGT 
ACTGTCGGCA GAAGAAACAC TTTCATTGGC ACTCCATACT GGATGGCCCC AGAAGTCATT 
GCCTGTGATG AAAATCCCGA CTCCACCTAT GATTACAGGA GTGATATCTG GTCTCTGGGA 
ATCACTGCTA TAGAAATGGC TGAAGGAGCA CCTCCGTTGT GTGACATGCA TCCCATGCGA 
GCTTTGTTCC TTATCCCACG GAACCCTCCT CCCAAACTGA AGTCCAAGAA ATGGTCTAAG 
AAGTTCATTG ACTTCATCGA GGGCTGTCTG GTAAAGTCAT ATCCGAATCG TCCGTCCACT 
GAACAGCTCC TGAAACACTC ATTCATCAGA GATCAGCCCA CTGAAAGACA AGTCCGCATC 
CAGCTCAAAG ATCACATCGA CAGAACACGC AAGAAACGTG GCGAAAAAGA GGAGACAGAG 
TACGAGTACA GCGGCAGTGA TGAAGAGGAT GAGAACAGAG GAGATGAGAG AGAATCCAGC 
TCTATTTTGA ATGTTCCTGG TGAGTCGACG TTGCGGAGGG ATTTCCTGCG GCTGCAGCAA 
GAAAATAAAG AGCGTTCAGA GGCCTTAAAG AGACAGCAGG CGCAGCTCGC CGCTCAGCGC 
AGAGACCCTG AGGAGCATAA ACGCCAGCTG CTGCACGACC GGCAGAAGCG CATAGAGGAG 
CAGAAAGAGC AGAGGCGACG CTTAGAGGAG CAACAGAGGA AGGAGAGAGA GATGGTGCGG 
CAACAACAGG ATAAAGCTCC TCACCGGAGA CTCGATGACA TGAGACGAGA GGAGGACAGG 
AGGTTGGCAG AGAGAGAGCA GGAGTATAAG CGTAAACAGC TGGAGGAGCA GAGACAGTCA 
GAGAGGCTTC AGAGGCAGTT ACAGCAGGAA CATGCGTATC TGGTCTCTCT ACAGCAACAG 
CAGCAGCAGC AAGACAAGAA ACCACAGATG TATCACTACA GCAAGAACCT AGAGAACAAC 
AAACCAGCCT GGGCTAGAGA GGTCGAGGAG CGCAGTAAGC TGAACAGACA GGGCTCTCCC 
AAAATCTGCA CCACGGTCTC TGACACTAAT ATTCAATCCC GCTCTGAGTC AATCAACCAA 
TCAGGAGCTG CGCAGACGCC GCCCATGCAG AGACCTGTGG AGCCGCAGGG TGGACAGGGA 
AAGTTCCAGA TGGCCCATCT AGTGCCGTTA AAACCATACG CCGCTCCAGT CCCTCGCTCT 
CAGTCTCTTT GTGATCAGCC CACTAAGACC ATGTCCGCCT TCCCCACCCA GGACCCCTCA 
CCCACACCCA CACCTCGTCC TCTACACTCC AGAGAACTAG TCCGTCAAAA CTCTGACCCC 
ACCTCAGAGA CTCCTGCCCC TCAGTCCCGG CCAATGAGAG ACGACCGCGG ACCCTGGATC 
CGTCTTCCTG AGATTGAACA ACCTCCTAAA ATTCCTCAGA GAACAGCCTC TATAGCCACT 
GCACTGAACA CAAATCTCTC CTCTGGTATC AGACACCCAG TCCGAGCAAG TAATCCAGAT 
CTGAGTCGCA ATGAGCGCTG GGAGAGAGGA GACAGCATGA GCATCATGTC CAACCTGCCA 
CAGACCGGCT CTCTGGAGAG ACACCGCATA CTCAGCTCAT CTAAGATGGA TTCCTCTCCA 
ACCCTCTCTC ATGATGGCCG ACACAAACCA GGAGAATCCC GAACCTCATC TAGACCCAGC 
AGACCAGCGA GCTATAAGAG AGCAATAGGA GAGGATCATG GTCTGTACTC TAAGGAGCGA 
GCAGAGGAGC AGCCGCGGCC CCCTGTGAAG GCCAACGACT ACTCCTCTTC CTCAGAGAGC 
AGTGAAAGCA GCGAAGAGAG TGAAAGTGGA GAAGGAGCAG AGGAGGAAAG CCCTACTGAC 
CGCCCCCGTG ATGCAGATTC TGACTCTGTG AACACTATGG TTGTCCATGA AGAGGAGGAA 
GGTGAAGGAG GAGAAGAAGA ACGTGCTGGA GGATATGGAG ACCAAACCAT GCTTGTGCAG 
AGGACTCCAG AGAAGCGCAG TCATAATGGA TACACCAACC TGCCTGATGT GGTTCAGCCT 
TCTCACTCTC CCACAGACTC GGCCTCCCAC TCCTCCCCCG GGAAAGACTC TACTTATGAT 
TATCAGTCCA GGGGATTGGT CAAGGCCTCT GGAAAATCCT CTTTTACTAC CTTTGTGGAC 
CTTGGAATGT ACCAGCCATC AGGGGGTAGT GGAGATAACA TTTCTGTTGG AGGTCTGGGC 
TCTCGTTTTG AGCAGCTAAA GCTGGAGGTG AGGAAGGGCT CCATGGTCAA TGTCAATCCC 
ACAAACACAC GTCCGGTCAG TGACACTCCT GAAATCCGCA AATACAAGAA GAGATTTAAC 
TCTGAGATCC TCTGCGCTGC TCTCTGGGGT GTGAATCTGC TGGTCGGGAC AGAGAATGGT 
CTGAAGCTGC TGGACAGAAG CGGTCAGGGT AAAGTGTATC CACTGATCAA CTCGCGCAGA 
TTTCAGCAGA TGGATGTCCT GGAGGGACTG AACCTCCTCA TCACTATATC AGGCAAGAAG 
AATAAGGTAC GCGTGTATTA TCTGGCCTGG CTGAGGAATA AGATCCTCCA TAATGACCCA 
GAGGTGGAGA AGAAACAGGG CTGGACCACT GTAGGGGAAA TGGAGGGCTG TGTGCACTAT 
AAAGTGGTGA AATATGAAAG GATTAAGTTT CTGGTTATCG CTCTGAAGAA TGCAGTGGAG 
GTTTACGCCT GGGCTCCAAA ACCTTACCAC AAGTTCATGG CCTTCAAGTC ATTTGGAGAC 
CTGCCGCACA GACCTGTGTT GGTTGACCTC ACAGTGGAAG AGGGTCAGAG GTTAAAGGTC 
ATCTATGGCT CCTTGGCTGG CTTCCATGCC ATTGATGTTG ACTCTGGAAA CAACTACGAC 
ATCTACATTC CTGTACATAT CCAGTCACAG ATCACTCCTC ACGCCATCGT GTTCCTGCCC 
AACTCAGATG GGATGGAGAT GCTGTTGTGT TATGAGGACG AAGGTGTTTA TGTCAACACG 
TACGGACGCA TCATTAAAGA TGTGGTGCTG CAGTGGGGAG AAATGCCCAC ATCAGTAGCC 
CACATTTGCT CTAATCAGAT TATGGGTTGG GGAGAGAAAG CCATTGAGAT CCGTTCTGTT 
GAGACGGGAC ACCTGGATGG AGTCTTCATG CACAAGAGAG CCCAGAGGCT TAAGTTCCTG 
TGTGAACGGA ACGACAAGGT GTTTTTCGCG TCAGTGCGTT CCGGTGGCAG CAGCCAAGTT 
TACTTCATGA CCCTCAACAG AAACTGCATT ATGAACTGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001300770.1
CDS	467..4288
Translation	MSENAPTRSLDDIDLAALRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEEEEEEIKAEINMLKKYSHHRNIATYYGAFVKKSPPGHDDQLWLVMEFCGAGSVTDLVKNTKGNSLKEDWIAYICREILRGLSHLHAHKVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDSTYDYRSDIWSLGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPKLKSKKWSKKFIDFIEGCLVKSYPNRPSTEQLLKHSFIRDQPTERQVRIQLKDHIDRTRKKRGEKEETEYEYSGSDEEDENRGDERESSSILNVPGESTLRRDFLRLQQENKERSEALKRQQAQLAAQRRDPEEHKRQLLHDRQKRIEEQKEQRRRLEEQQRKEREMVRQQQDKAPHRRLDDMRREEDRRLAEREQEYKRKQLEEQRQSERLQRQLQQEHAYLVSLQQQQQQQDKKPQMYHYSKNLENNKPAWAREVEERSKLNRQGSPKICTTVSDTNIQSRSESINQSGAAQTPPMQRPVEPQGGQGKFQMAHLVPLKPYAAPVPRSQSLCDQPTKTMSAFPTQDPSPTPTPRPLHSRELVRQNSDPTSETPAPQSRPMRDDRGPWIRLPEIEQPPKIPQRTASIATALNTNLSSGIRHPVRASNPDLSRNERWERGDSMSIMSNLPQTGSLERHRILSSSKMDSSPTLSHDGRHKPGESRTSSRPSRPASYKRAIGEDHGLYSKERAEEQPRPPVKANDYSSSSESSESSEESESGEGAEEESPTDRPRDADSDSVNTMVVHEEEEGEGGEEERAGGYGDQTMLVQRTPEKRSHNGYTNLPDVVQPSHSPTDSASHSSPGKDSTYDYQSRGLVKASGKSSFTTFVDLGMYQPSGGSGDNISVGGLGSRFEQLKLEVRKGSMVNVNPTNTRPVSDTPEIRKYKKRFNSEILCAALWGVNLLVGTENGLKLLDRSGQGKVYPLINSRRFQQMDVLEGLNLLITISGKKNKVRVYYLAWLRNKILHNDPEVEKKQGWTTVGEMEGCVHYKVVKYERIKFLVIALKNAVEVYAWAPKPYHKFMAFKSFGDLPHRPVLVDLTVEEGQRLKVIYGSLAGFHAIDVDSGNNYDIYIPVHIQSQITPHAIVFLPNSDGMEMLLCYEDEGVYVNTYGRIIKDVVLQWGEMPTSVAHICSNQIMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLNRNCIMNW

Target ORF information:

RefSeq Version	NM_001313841.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio misshapen-like kinase 1 (mink1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001313841.1

ORF Insert Sequence:

ATGTCAGAAA ACGCACCTAC GCGGAGTCTG GATGACATCG ACCTGGCCGC CCTGCGGGAC 
CCAGCAGGGA TCTTCGAGTT GGTGGAGGTC GTGGGCAACG GCACCTATGG ACAAGTTTAC 
AAGGGTCGTC ATGTTAAGAC AGGTCAGCTG GCAGCCATAA AAGTCATGGA TGTGACTGAG 
GAAGAAGAGG AGGAGATTAA AGCGGAGATT AACATGCTGA AGAAGTACAG TCACCATAGA 
AATATCGCCA CCTACTATGG AGCCTTTGTG AAGAAGAGTC CTCCTGGACA CGATGACCAG 
CTCTGGTTGG TGATGGAGTT TTGTGGTGCC GGCTCAGTAA CAGACTTGGT GAAGAACACA 
AAAGGCAACT CTTTAAAAGA AGACTGGATC GCCTATATCT GCAGAGAGAT CCTTAGGGGT 
CTGTCTCACC TCCATGCTCA TAAAGTCATC CACCGAGACA TCAAGGGACA GAATGTGCTG 
CTTACAGAGA ACGCAGAGGT TAAACTGGTG GATTTTGGGG TGAGCGCACA GTTGGATCGT 
ACTGTCGGCA GAAGAAACAC TTTCATTGGC ACTCCATACT GGATGGCCCC AGAAGTCATT 
GCCTGTGATG AAAATCCCGA CTCCACCTAT GATTACAGGA GTGATATCTG GTCTCTGGGA 
ATCACTGCTA TAGAAATGGC TGAAGGAGCA CCTCCGTTGT GTGACATGCA TCCCATGCGA 
GCTTTGTTCC TTATCCCACG GAACCCTCCT CCCAAACTGA AGTCCAAGAA ATGGTCTAAG 
AAGTTCATTG ACTTCATCGA GGGCTGTCTG GTAAAGTCAT ATCCGAATCG TCCGTCCACT 
GAACAGCTCC TGAAACACTC ATTCATCAGA GATCAGCCCA CTGAAAGACA AGTCCGCATC 
CAGCTCAAAG ATCACATCGA CAGAACACGC AAGAAACGTG GCGAAAAAGA GGAGACAGAG 
TACGAGTACA GCGGCAGTGA TGAAGAGGAT GAGAACAGAG GAGATGAGAG AGAATCCAGC 
TCTATTTTGA ATGTTCCTGG TGAGTCGACG TTGCGGAGGG ATTTCCTGCG GCTGCAGCAA 
GAAAATAAAG AGCGTTCAGA GGCCTTAAAG AGACAGCAGG CGCAGCTCGC CGCTCAGCGC 
AGAGACCCTG AGGAGCATAA ACGCCAGCTG CTGCACGACC GGCAGAAGCG CATAGAGGAG 
CAGAAAGAGC AGAGGCGACG CTTAGAGGAG CAACAGAGGA AGGAGAGAGA GATGGTGCGG 
CAACAACAGG ATAAAGCTCC TCACCGGAGA CTCGATGACA TGAGACGAGA GGAGGACAGG 
AGGTTGGCAG AGAGAGAGCA GGAGTATAAG CGTAAACAGC TGGAGGAGCA GAGACAGTCA 
GAGAGGCTTC AGAGGCAGTT ACAGCAGGAA CATGCGTATC TGGTCTCTCT ACAGCAACAG 
CAGCAGCAGC AAGACAAGAA ACCACAGATG TATCACTACA GCAAGAACCT AGAGAACAAC 
AAACCAGCCT GGGCTAGAGA GGTCGAGGAG CGCAGTAAGC TGAACAGACA GGGCTCTCCC 
AAAATCTGCA CCACGGTCTC TGACACTAAT ATTCAATCCC GCTCTGAGTC AATCAACCAA 
TCAGGAGCTG CGCAGACGCC GCCCATGCAG AGACCTGTGG AGCCGCAGGG TGGACAGGGA 
AAGTTCCAGA TGGCCCATCT AGTGCCGTTA AAACCATACG CCGCTCCAGT CCCTCGCTCT 
CAGTCTCTTT GTGATCAGCC CACTAAGACC ATGTCCGCCT TCCCCACCCA GGACCCCTCA 
CCCACACCCA CACCTCGTCC TCTACACTCC AGAGAACTAG TCCGTCAAAA CTCTGACCCC 
ACCTCAGAGA CTCCTGCCCC TCAGTCCCGG CCAATGAGAG ACGACCGCGG ACCCTGGATC 
CGTCTTCCTG AGATTGAACA ACCTCCTAAA ATTCCTCAGA GAACAGCCTC TATAGCCACT 
GCACTGAACA CAAATCTCTC CTCTGGTATC AGACACCCAG TCCGAGCAAG TAATCCAGAT 
CTGAGTCGCA ATGAGCGCTG GGAGAGAGGA GACAGCATGA GCATCATGTC CAACCTGCCA 
CAGACCGGCT CTCTGGAGAG ACACCGCATA CTCAGCTCAT CTAAGATGGA TTCCTCTCCA 
ACCCTCTCTC ATGATGGCCG ACACAAACCA GGAGAATCCC GAACCTCATC TAGACCCAGC 
AGACCAGCGA GCTATAAGAG AGCAATAGGA GAGGATCATG GTCTGTACTC TAAGGAGCGA 
GCAGAGGAGC AGCCGCGGCC CCCTGTGAAG GCCAACGACT ACTCCTCTTC CTCAGAGAGC 
AGTGAAAGCA GCGAAGAGAG TGAAAGTGGA GAAGGAGCAG AGGAGGAAAG CCCTACTGAC 
CGCCCCCGTG ATGCAGATTC TGACTCTGTG AACACTATGG TTGTCCATGA AGAGGAGGAA 
GGTGAAGGAG GAGAAGAAGA ACGTGCTGGA GGATATGGAG ACCAAACCAT GCTTGTGCAG 
AGGACTCCAG AGAAGCGCAG TCATAATGGA TACACCAACC TGCCTGATGT GGTTCAGCCT 
TCTCACTCTC CCACAGACTC GGCCTCCCAC TCCTCCCCCG GGAAAGACTC TACTTATGAT 
TATCAGTCCA GGGGATTGGT CAAGGCCTCT GGAAAATCCT CTTTTACTAC CTTTGTGGAC 
CTTGGAATGT ACCAGCCATC AGGGGGTAGT GGAGATAACA TTTCTGTTGG AGGTCTGGGC 
TCTCGTTTTG AGCAGCTAAA GCTGGAGGTG AGGAAGGGCT CCATGGTCAA TGTCAATCCC 
ACAAACACAC GTCCGGTCAG TGACACTCCT GAAATCCGCA AATACAAGAA GAGATTTAAC 
TCTGAGATCC TCTGCGCTGC TCTCTGGGGT GTGAATCTGC TGGTCGGGAC AGAGAATGGT 
CTGAAGCTGC TGGACAGAAG CGGTCAGGGT AAAGTGTATC CACTGATCAA CTCGCGCAGA 
TTTCAGCAGA TGGATGTCCT GGAGGGACTG AACCTCCTCA TCACTATATC AGGCAAGAAG 
AATAAGGTAC GCGTGTATTA TCTGGCCTGG CTGAGGAATA AGATCCTCCA TAATGACCCA 
GAGGTGGAGA AGAAACAGGG CTGGACCACT GTAGGGGAAA TGGAGGGCTG TGTGCACTAT 
AAAGTGGTGA AATATGAAAG GATTAAGTTT CTGGTTATCG CTCTGAAGAA TGCAGTGGAG 
GTTTACGCCT GGGCTCCAAA ACCTTACCAC AAGTTCATGG CCTTCAAGTC ATTTGGAGAC 
CTGCCGCACA GACCTGTGTT GGTTGACCTC ACAGTGGAAG AGGGTCAGAG GTTAAAGGTC 
ATCTATGGCT CCTTGGCTGG CTTCCATGCC ATTGATGTTG ACTCTGGAAA CAACTACGAC 
ATCTACATTC CTGTACATAT CCAGTCACAG ATCACTCCTC ACGCCATCGT GTTCCTGCCC 
AACTCAGATG GGATGGAGAT GCTGTTGTGT TATGAGGACG AAGGTGTTTA TGTCAACACG 
TACGGACGCA TCATTAAAGA TGTGGTGCTG CAGTGGGGAG AAATGCCCAC ATCAGTAGCC 
CACATTTGCT CTAATCAGAT TATGGGTTGG GGAGAGAAAG CCATTGAGAT CCGTTCTGTT 
GAGACGGGAC ACCTGGATGG AGTCTTCATG CACAAGAGAG CCCAGAGGCT TAAGTTCCTG 
TGTGAACGGA ACGACAAGGT GTTTTTCGCG TCAGTGCGTT CCGGTGGCAG CAGCCAAGTT 
TACTTCATGA CCCTCAACAG AAACTGCATT ATGAACTGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mink1 ( XM_021475970.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021331645.1 Danio rerio misshapen-like kinase 1 (mink1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa142547
Clone ID Related Accession (Same CDS sequence)	XM_021475970.1
Accession Version	XM_021475970.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3945bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	misshapen-like kinase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGAAA ACGCACCTAC GCGGAGTCTG GATGACATCG ACCTGGCCGC CCTGCGGGAC 
CCAGCAGGGA TCTTCGAGTT GGTGGAGGTC GTGGGCAACG GCACCTATGG ACAAGTTTAC 
AAGGGTCGTC ATGTTAAGAC AGGTCAGCTG GCAGCCATAA AAGTCATGGA TGTGACTGAG 
GAAGAAGAGG AGGAGATTAA AGCGGAGATT AACATGCTGA AGAAGTACAG TCACCATAGA 
AATATCGCCA CCTACTATGG AGCCTTTGTG AAGAAGAGTC CTCCTGGACA CGATGACCAG 
CTCTGGTTGG TGATGGAGTT TTGTGGTGCC GGCTCAGTAA CAGACTTGGT GAAGAACACA 
AAAGGCAACT CTTTAAAAGA AGACTGGATC GCCTATATCT GCAGAGAGAT CCTTAGGGGT 
CTGTCTCACC TCCATGCTCA TAAAGTCATC CACCGAGACA TCAAGGGACA GAATGTGCTG 
CTTACAGAGA ACGCAGAGGT TAAACTGGTG GATTTTGGGG TGAGCGCACA GTTGGATCGT 
ACTGTCGGCA GAAGAAACAC TTTCATTGGC ACTCCATACT GGATGGCCCC AGAAGTCATT 
GCCTGTGATG AAAATCCCGA CTCCACCTAT GATTACAGGA GTGATATCTG GTCTCTGGGA 
ATCACTGCTA TAGAAATGGC TGAAGGAGCA CCTCCGTTGT GTGACATGCA TCCCATGCGA 
GCTTTGTTCC TTATCCCACG GAACCCTCCT CCCAAACTGA AGTCCAAGAA ATGGTCTAAG 
AAGTTCATTG ACTTCATCGA GGGCTGTCTG GTAAAGTCAT ATCCGAATCG TCCGTCCACT 
GAACAGCTCC TGAAACACTC ATTCATCAGA GATCAGCCCA CTGAAAGACA AGTCCGCATC 
CAGCTCAAAG ATCACATCGA CAGAACACGC AAGAAACGTG GCGAAAAAGA GGAGACAGAG 
TACGAGTACA GCGGCAGTGA TGAAGAGGAT GAGAACAGAG GAGATGAGAG AGAATCCAGC 
TCTATTTTGA ATGTTCCTGG TGAGTCGACG TTGCGGAGGG ATTTCCTGCG GCTGCAGCAA 
GAAAATAAAG AGCGTTCAGA GGCCTTAAAG AGACAGCAGG CGCAGCTCGC CGCTCAGCGC 
AGAGACCCTG AGGAGCATAA ACGCCAGCTG CTGCACGACC GGCAGAAGCG CATAGAGGAG 
CAGAAAGAGC AGAGGCGACG CTTAGAGGAG CAACAGAGGA AGGAGAGAGA GATGGTGCGG 
CAACAACAGG ATAAAGCTCC TCACCGGAGA CTCGATGACA TGAGACGAGA GGAGGACAGG 
AGGTTGGCAG AGAGAGAGCA GGAGTTTATA AGACATAAGT TGGAGGAAGA GCAGCGGCAG 
TTAGAGATAC TGCAGCAGCA ATTACTGCAG GAGCAGGCAT TGCTAATGTT GTACAATAAA 
CACTGCTCAG ACTCTTCAGT AAATGAGTAT AAGCGTAAAC AGCTGGAGGA GCAGAGACAG 
TCAGAGAGGC TTCAGAGGCA GTTACAGCAG GAACATGCGT ATCTGGTCTC TCTACAGCAA 
CAGCAGCAGC AGCAAGACAA GAAACCACAG ATGTATCACT ACAGCAAGAA CCTAGAGAAC 
AACAAACCAG CCTGGGCTAG AGAGGTCGAG GAGCGCAGTA AGCTGAACAG ACAGGGCTCT 
CCCAAAATCT GCACCACGGT CTCTGACACT AATATTCAAT CCCGCTCTGA GTCAATCAAC 
CAATCAGGAG CTGCGCAGAC GCCGCCCATG CAGAGACCTG TGGAGCCGCA GGGTGGACAG 
GGAAAGTTCC AGATGGCCCA TCTAGTGCCG TTAAAACCAT ACGCCGCTCC AGTCCCTCGC 
TCTCAGTCTC TTTGTGATCA GCCCACTAAG ACCATGTCCG CCTTCCCCAC CCAGGACCCC 
TCACCCACAC CCACACCTCG TCCTCTACAC TCCAGAGAAC TAGTCCGTCA AAACTCTGAC 
CCCACCTCAG AGACTCCTGC CCCTCAGTCC CGGCCAATGA GAGACGACCG CGGACCCTGG 
ATCCGTCTTC CTGAGATTGA ACAACCTCCT AAAATTCCTC AGAGAACAGC CTCTATAGCC 
ACTGCACTGA ACACAAATCT CTCCTCTGGT ATCAGACACC CAGTCCGAGC AAGTAATCCA 
GATCTGAGTC GCAATGAGCG CTGGGAGAGA GGAGACAGCA TGAGCATCAT GTCCAACCTG 
CCACAGACCG GCTCTCTGGA GAGACACCGC ATACTCAGCT CATCTAAGAT GGATTCCTCT 
CCAACCCTCT CTCATGATGG CCGACACAAA CCAGGAGAAT CCCGAACCTC ATCTAGACCC 
AGCAGACCAG CGAGCTATAA GAGAGCAATA GGAGAGGATC ATGGTCTGTA CTCTAAGGAG 
CGAGCAGAGG AGCAGCCGCG GCCCCCTGTG AAGGCCAACG ACTACTCCTC TTCCTCAGAG 
AGCAGTGAAA GCAGCGAAGA GAGTGAAAGT GGAGAAGGAG CAGAGGAGGA AAGCCCTACT 
GACCGCCCCC GTGATGCAGA TTCTGACTCT GTGAACACTA TGGTTGTCCA TGAAGAGGAG 
GAAGGTGAAG GAGGAGAAGA AGAACGTGCT GGAGGATATG GAGACCAAAC CATGCTTGTG 
CAGAGGACTC CAGAGAAGCG CAGTCATAAT GGATACACCA ACCTGCCTGA TGTGGTTCAG 
CCTTCTCACT CTCCCACAGA CTCGGCCTCC CACTCCTCCC CCGGGAAAGA CTCTACTTAT 
GATTATCAGT CCAGGGGATT GGTCAAGGCC TCTGGAAAAT CCTCTTTTAC TACCTTTGTG 
GACCTTGGAA TGTACCAGCC ATCAGGGGGT AGTGGAGATA ACATTTCTGT TGGAGGTCTG 
GGCTCTCGTT TTGAGCAGCT AAAGCTGGAG GTGAGGAAGG GCTCCATGGT CAATGTCAAT 
CCCACAAACA CACGTCCGGT CAGTGACACT CCTGAAATCC GCAAATACAA GAAGAGATTT 
AACTCTGAGA TCCTCTGCGC TGCTCTCTGG GGTGTGAATC TGCTGGTCGG GACAGAGAAT 
GGTCTGAAGC TGCTGGACAG AAGCGGTCAG GGTAAAGTGT ATCCACTGAT CAACTCGCGC 
AGATTTCAGC AGATGGATGT CCTGGAGGGA CTGAACCTCC TCATCACTAT ATCAGGCAAG 
AAGAATAAGG TACGCGTGTA TTATCTGGCC TGGCTGAGGA ATAAGATCCT CCATAATGAC 
CCAGAGGTGG AGAAGAAACA GGGCTGGACC ACTGTAGGGG AAATGGAGGG CTGTGTGCAC 
TATAAAGTGG TGAAATATGA AAGGATTAAG TTTCTGGTTA TCGCTCTGAA GAATGCAGTG 
GAGGTTTACG CCTGGGCTCC AAAACCTTAC CACAAGTTCA TGGCCTTCAA GTCATTTGGA 
GACCTGCCGC ACAGACCTGT GTTGGTTGAC CTCACAGTGG AAGAGGGTCA GAGGTTAAAG 
GTCATCTATG GCTCCTTGGC TGGCTTCCAT GCCATTGATG TTGACTCTGG AAACAACTAC 
GACATCTACA TTCCTGTACA TATCCAGTCA CAGATCACTC CTCACGCCAT CGTGTTCCTG 
CCCAACTCAG ATGGGATGGA GATGCTGTTG TGTTATGAGG ACGAAGGTGT TTATGTCAAC 
ACGTACGGAC GCATCATTAA AGATGTGGTG CTGCAGTGGG GAGAAATGCC CACATCAGTA 
GCCCACATTT GCTCTAATCA GATTATGGGT TGGGGAGAGA AAGCCATTGA GATCCGTTCT 
GTTGAGACGG GACACCTGGA TGGAGTCTTC ATGCACAAGA GAGCCCAGAG GCTTAAGTTC 
CTGTGTGAAC GGAACGACAA GGTGTTTTTC GCGTCAGTGC GTTCCGGTGG CAGCAGCCAA 
GTTTACTTCA TGACCCTCAA CAGAAACTGC ATTATGAACT GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021331645.1
CDS	1263..5207
Translation	MSENAPTRSLDDIDLAALRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEEEEEEIKAEINMLKKYSHHRNIATYYGAFVKKSPPGHDDQLWLVMEFCGAGSVTDLVKNTKGNSLKEDWIAYICREILRGLSHLHAHKVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDSTYDYRSDIWSLGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPKLKSKKWSKKFIDFIEGCLVKSYPNRPSTEQLLKHSFIRDQPTERQVRIQLKDHIDRTRKKRGEKEETEYEYSGSDEEDENRGDERESSSILNVPGESTLRRDFLRLQQENKERSEALKRQQAQLAAQRRDPEEHKRQLLHDRQKRIEEQKEQRRRLEEQQRKEREMVRQQQDKAPHRRLDDMRREEDRRLAEREQEFIRHKLEEEQRQLEILQQQLLQEQALLMLYNKHCSDSSVNEYKRKQLEEQRQSERLQRQLQQEHAYLVSLQQQQQQQDKKPQMYHYSKNLENNKPAWAREVEERSKLNRQGSPKICTTVSDTNIQSRSESINQSGAAQTPPMQRPVEPQGGQGKFQMAHLVPLKPYAAPVPRSQSLCDQPTKTMSAFPTQDPSPTPTPRPLHSRELVRQNSDPTSETPAPQSRPMRDDRGPWIRLPEIEQPPKIPQRTASIATALNTNLSSGIRHPVRASNPDLSRNERWERGDSMSIMSNLPQTGSLERHRILSSSKMDSSPTLSHDGRHKPGESRTSSRPSRPASYKRAIGEDHGLYSKERAEEQPRPPVKANDYSSSSESSESSEESESGEGAEEESPTDRPRDADSDSVNTMVVHEEEEGEGGEEERAGGYGDQTMLVQRTPEKRSHNGYTNLPDVVQPSHSPTDSASHSSPGKDSTYDYQSRGLVKASGKSSFTTFVDLGMYQPSGGSGDNISVGGLGSRFEQLKLEVRKGSMVNVNPTNTRPVSDTPEIRKYKKRFNSEILCAALWGVNLLVGTENGLKLLDRSGQGKVYPLINSRRFQQMDVLEGLNLLITISGKKNKVRVYYLAWLRNKILHNDPEVEKKQGWTTVGEMEGCVHYKVVKYERIKFLVIALKNAVEVYAWAPKPYHKFMAFKSFGDLPHRPVLVDLTVEEGQRLKVIYGSLAGFHAIDVDSGNNYDIYIPVHIQSQITPHAIVFLPNSDGMEMLLCYEDEGVYVNTYGRIIKDVVLQWGEMPTSVAHICSNQIMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLNRNCIMNW

Target ORF information:

RefSeq Version	XM_021475970.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio misshapen-like kinase 1 (mink1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021475970.1

ORF Insert Sequence:

ATGTCAGAAA ACGCACCTAC GCGGAGTCTG GATGACATCG ACCTGGCCGC CCTGCGGGAC 
CCAGCAGGGA TCTTCGAGTT GGTGGAGGTC GTGGGCAACG GCACCTATGG ACAAGTTTAC 
AAGGGTCGTC ATGTTAAGAC AGGTCAGCTG GCAGCCATAA AAGTCATGGA TGTGACTGAG 
GAAGAAGAGG AGGAGATTAA AGCGGAGATT AACATGCTGA AGAAGTACAG TCACCATAGA 
AATATCGCCA CCTACTATGG AGCCTTTGTG AAGAAGAGTC CTCCTGGACA CGATGACCAG 
CTCTGGTTGG TGATGGAGTT TTGTGGTGCC GGCTCAGTAA CAGACTTGGT GAAGAACACA 
AAAGGCAACT CTTTAAAAGA AGACTGGATC GCCTATATCT GCAGAGAGAT CCTTAGGGGT 
CTGTCTCACC TCCATGCTCA TAAAGTCATC CACCGAGACA TCAAGGGACA GAATGTGCTG 
CTTACAGAGA ACGCAGAGGT TAAACTGGTG GATTTTGGGG TGAGCGCACA GTTGGATCGT 
ACTGTCGGCA GAAGAAACAC TTTCATTGGC ACTCCATACT GGATGGCCCC AGAAGTCATT 
GCCTGTGATG AAAATCCCGA CTCCACCTAT GATTACAGGA GTGATATCTG GTCTCTGGGA 
ATCACTGCTA TAGAAATGGC TGAAGGAGCA CCTCCGTTGT GTGACATGCA TCCCATGCGA 
GCTTTGTTCC TTATCCCACG GAACCCTCCT CCCAAACTGA AGTCCAAGAA ATGGTCTAAG 
AAGTTCATTG ACTTCATCGA GGGCTGTCTG GTAAAGTCAT ATCCGAATCG TCCGTCCACT 
GAACAGCTCC TGAAACACTC ATTCATCAGA GATCAGCCCA CTGAAAGACA AGTCCGCATC 
CAGCTCAAAG ATCACATCGA CAGAACACGC AAGAAACGTG GCGAAAAAGA GGAGACAGAG 
TACGAGTACA GCGGCAGTGA TGAAGAGGAT GAGAACAGAG GAGATGAGAG AGAATCCAGC 
TCTATTTTGA ATGTTCCTGG TGAGTCGACG TTGCGGAGGG ATTTCCTGCG GCTGCAGCAA 
GAAAATAAAG AGCGTTCAGA GGCCTTAAAG AGACAGCAGG CGCAGCTCGC CGCTCAGCGC 
AGAGACCCTG AGGAGCATAA ACGCCAGCTG CTGCACGACC GGCAGAAGCG CATAGAGGAG 
CAGAAAGAGC AGAGGCGACG CTTAGAGGAG CAACAGAGGA AGGAGAGAGA GATGGTGCGG 
CAACAACAGG ATAAAGCTCC TCACCGGAGA CTCGATGACA TGAGACGAGA GGAGGACAGG 
AGGTTGGCAG AGAGAGAGCA GGAGTTTATA AGACATAAGT TGGAGGAAGA GCAGCGGCAG 
TTAGAGATAC TGCAGCAGCA ATTACTGCAG GAGCAGGCAT TGCTAATGTT GTACAATAAA 
CACTGCTCAG ACTCTTCAGT AAATGAGTAT AAGCGTAAAC AGCTGGAGGA GCAGAGACAG 
TCAGAGAGGC TTCAGAGGCA GTTACAGCAG GAACATGCGT ATCTGGTCTC TCTACAGCAA 
CAGCAGCAGC AGCAAGACAA GAAACCACAG ATGTATCACT ACAGCAAGAA CCTAGAGAAC 
AACAAACCAG CCTGGGCTAG AGAGGTCGAG GAGCGCAGTA AGCTGAACAG ACAGGGCTCT 
CCCAAAATCT GCACCACGGT CTCTGACACT AATATTCAAT CCCGCTCTGA GTCAATCAAC 
CAATCAGGAG CTGCGCAGAC GCCGCCCATG CAGAGACCTG TGGAGCCGCA GGGTGGACAG 
GGAAAGTTCC AGATGGCCCA TCTAGTGCCG TTAAAACCAT ACGCCGCTCC AGTCCCTCGC 
TCTCAGTCTC TTTGTGATCA GCCCACTAAG ACCATGTCCG CCTTCCCCAC CCAGGACCCC 
TCACCCACAC CCACACCTCG TCCTCTACAC TCCAGAGAAC TAGTCCGTCA AAACTCTGAC 
CCCACCTCAG AGACTCCTGC CCCTCAGTCC CGGCCAATGA GAGACGACCG CGGACCCTGG 
ATCCGTCTTC CTGAGATTGA ACAACCTCCT AAAATTCCTC AGAGAACAGC CTCTATAGCC 
ACTGCACTGA ACACAAATCT CTCCTCTGGT ATCAGACACC CAGTCCGAGC AAGTAATCCA 
GATCTGAGTC GCAATGAGCG CTGGGAGAGA GGAGACAGCA TGAGCATCAT GTCCAACCTG 
CCACAGACCG GCTCTCTGGA GAGACACCGC ATACTCAGCT CATCTAAGAT GGATTCCTCT 
CCAACCCTCT CTCATGATGG CCGACACAAA CCAGGAGAAT CCCGAACCTC ATCTAGACCC 
AGCAGACCAG CGAGCTATAA GAGAGCAATA GGAGAGGATC ATGGTCTGTA CTCTAAGGAG 
CGAGCAGAGG AGCAGCCGCG GCCCCCTGTG AAGGCCAACG ACTACTCCTC TTCCTCAGAG 
AGCAGTGAAA GCAGCGAAGA GAGTGAAAGT GGAGAAGGAG CAGAGGAGGA AAGCCCTACT 
GACCGCCCCC GTGATGCAGA TTCTGACTCT GTGAACACTA TGGTTGTCCA TGAAGAGGAG 
GAAGGTGAAG GAGGAGAAGA AGAACGTGCT GGAGGATATG GAGACCAAAC CATGCTTGTG 
CAGAGGACTC CAGAGAAGCG CAGTCATAAT GGATACACCA ACCTGCCTGA TGTGGTTCAG 
CCTTCTCACT CTCCCACAGA CTCGGCCTCC CACTCCTCCC CCGGGAAAGA CTCTACTTAT 
GATTATCAGT CCAGGGGATT GGTCAAGGCC TCTGGAAAAT CCTCTTTTAC TACCTTTGTG 
GACCTTGGAA TGTACCAGCC ATCAGGGGGT AGTGGAGATA ACATTTCTGT TGGAGGTCTG 
GGCTCTCGTT TTGAGCAGCT AAAGCTGGAG GTGAGGAAGG GCTCCATGGT CAATGTCAAT 
CCCACAAACA CACGTCCGGT CAGTGACACT CCTGAAATCC GCAAATACAA GAAGAGATTT 
AACTCTGAGA TCCTCTGCGC TGCTCTCTGG GGTGTGAATC TGCTGGTCGG GACAGAGAAT 
GGTCTGAAGC TGCTGGACAG AAGCGGTCAG GGTAAAGTGT ATCCACTGAT CAACTCGCGC 
AGATTTCAGC AGATGGATGT CCTGGAGGGA CTGAACCTCC TCATCACTAT ATCAGGCAAG 
AAGAATAAGG TACGCGTGTA TTATCTGGCC TGGCTGAGGA ATAAGATCCT CCATAATGAC 
CCAGAGGTGG AGAAGAAACA GGGCTGGACC ACTGTAGGGG AAATGGAGGG CTGTGTGCAC 
TATAAAGTGG TGAAATATGA AAGGATTAAG TTTCTGGTTA TCGCTCTGAA GAATGCAGTG 
GAGGTTTACG CCTGGGCTCC AAAACCTTAC CACAAGTTCA TGGCCTTCAA GTCATTTGGA 
GACCTGCCGC ACAGACCTGT GTTGGTTGAC CTCACAGTGG AAGAGGGTCA GAGGTTAAAG 
GTCATCTATG GCTCCTTGGC TGGCTTCCAT GCCATTGATG TTGACTCTGG AAACAACTAC 
GACATCTACA TTCCTGTACA TATCCAGTCA CAGATCACTC CTCACGCCAT CGTGTTCCTG 
CCCAACTCAG ATGGGATGGA GATGCTGTTG TGTTATGAGG ACGAAGGTGT TTATGTCAAC 
ACGTACGGAC GCATCATTAA AGATGTGGTG CTGCAGTGGG GAGAAATGCC CACATCAGTA 
GCCCACATTT GCTCTAATCA GATTATGGGT TGGGGAGAGA AAGCCATTGA GATCCGTTCT 
GTTGAGACGG GACACCTGGA TGGAGTCTTC ATGCACAAGA GAGCCCAGAG GCTTAAGTTC 
CTGTGTGAAC GGAACGACAA GGTGTTTTTC GCGTCAGTGC GTTCCGGTGG CAGCAGCCAA 
GTTTACTTCA TGACCCTCAA CAGAAACTGC ATTATGAACT GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mink1 ( XM_021475971.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021331646.1 Danio rerio misshapen-like kinase 1 (mink1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa142548
Clone ID Related Accession (Same CDS sequence)	XM_021475971.1
Accession Version	XM_021475971.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3939bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	misshapen-like kinase 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGAAA ACGCACCTAC GCGGAGTCTG GATGACATCG ACCTGGCCGC CCTGCGGGAC 
CCAGCAGGGA TCTTCGAGTT GGTGGAGGTC GTGGGCAACG GCACCTATGG ACAAGTTTAC 
AAGGGTCGTC ATGTTAAGAC AGGTCAGCTG GCAGCCATAA AAGTCATGGA TGTGACTGAG 
GAAGAAGAGG AGGAGATTAA AGCGGAGATT AACATGCTGA AGAAGTACAG TCACCATAGA 
AATATCGCCA CCTACTATGG AGCCTTTGTG AAGAAGAGTC CTCCTGGACA CGATGACCAG 
CTCTGGTTGG TGATGGAGTT TTGTGGTGCC GGCTCAGTAA CAGACTTGGT GAAGAACACA 
AAAGGCAACT CTTTAAAAGA AGACTGGATC GCCTATATCT GCAGAGAGAT CCTTAGGGGT 
CTGTCTCACC TCCATGCTCA TAAAGTCATC CACCGAGACA TCAAGGGACA GAATGTGCTG 
CTTACAGAGA ACGCAGAGGT TAAACTGGTG GATTTTGGGG TGAGCGCACA GTTGGATCGT 
ACTGTCGGCA GAAGAAACAC TTTCATTGGC ACTCCATACT GGATGGCCCC AGAAGTCATT 
GCCTGTGATG AAAATCCCGA CTCCACCTAT GATTACAGGA GTGATATCTG GTCTCTGGGA 
ATCACTGCTA TAGAAATGGC TGAAGGAGCA CCTCCGTTGT GTGACATGCA TCCCATGCGA 
GCTTTGTTCC TTATCCCACG GAACCCTCCT CCCAAACTGA AGTCCAAGAA ATGGTCTAAG 
AAGTTCATTG ACTTCATCGA GGGCTGTCTG GTAAAGTCAT ATCCGAATCG TCCGTCCACT 
GAACAGCTCC TGAAACACTC ATTCATCAGA GATCAGCCCA CTGAAAGACA AGTCCGCATC 
CAGCTCAAAG ATCACATCGA CAGAACACGC AAGAAACGTG GCGAAAAAGA GGAGACAGAG 
TACGAGTACA GCGGCAGTGA TGAAGAGGAT GAGAACAGAG GAGATGAGAG AGAATCCAGC 
TCTATTTTGA ATGTTCCTGG TGAGTCGACG TTGCGGAGGG ATTTCCTGCG GCTGCAGCAA 
GAAAATAAAG AGCGTTCAGA GGCCTTAAAG AGACAGCAGG CGCAGCTCGC CGCTCAGCGC 
AGAGACCCTG AGGAGCATAA ACGCCAGCTG CTGCACGACC GGCAGAAGCG CATAGAGGAG 
CAGAAAGAGC AGAGGCGACG CTTAGAGGAG CAACAGAGGA AGGAGAGAGA GATGGTGCGG 
CAACAACAGG ATAAAGCTCC TCACCGGAGA CTCGATGACA TGAGACGAGA GGAGGACAGG 
AGGTTGGCAG AGAGAGAGCA GGAGTTTATA AGACATAAGT TGGAGGAAGA GCAGCGGCAG 
TTAGAGATAC TGCAGCAGCA ATTACTGCAG GAGCAGGCAT TGCTAATGTT GTACAATAAA 
CACTGCTCAG ACTCTTCAGT AAATGAGTAT AAGCGTAAAC AGCTGGAGGA GCAGAGACAG 
TCAGAGAGGC TTCAGAGGCA GTTACAGCAG GAACATGCGT ATCTGGTCTC TCTACAGCAA 
CAGCAGCAGC AGCAAGACAA GAAACCACAG ATGTATCACT ACAGCAAGAA CCTAGAGAAC 
AACAAACCAG CCTGGGCTAG AGAGGTCGAG GAGCGCAGTA AGCTGAACAG ACAGGGCTCT 
CCCAAAATCT GCACCACGGT CTCTGACACT AATATTCAAT CCCGCTCTGA GTCAATCAAC 
CAATCAGGAG CTGCGCAGAC GCCGCCCATG CAGAGACCTG TGGAGCCGCA GGGTGGACAG 
GGAAAGATGG CCCATCTAGT GCCGTTAAAA CCATACGCCG CTCCAGTCCC TCGCTCTCAG 
TCTCTTTGTG ATCAGCCCAC TAAGACCATG TCCGCCTTCC CCACCCAGGA CCCCTCACCC 
ACACCCACAC CTCGTCCTCT ACACTCCAGA GAACTAGTCC GTCAAAACTC TGACCCCACC 
TCAGAGACTC CTGCCCCTCA GTCCCGGCCA ATGAGAGACG ACCGCGGACC CTGGATCCGT 
CTTCCTGAGA TTGAACAACC TCCTAAAATT CCTCAGAGAA CAGCCTCTAT AGCCACTGCA 
CTGAACACAA ATCTCTCCTC TGGTATCAGA CACCCAGTCC GAGCAAGTAA TCCAGATCTG 
AGTCGCAATG AGCGCTGGGA GAGAGGAGAC AGCATGAGCA TCATGTCCAA CCTGCCACAG 
ACCGGCTCTC TGGAGAGACA CCGCATACTC AGCTCATCTA AGATGGATTC CTCTCCAACC 
CTCTCTCATG ATGGCCGACA CAAACCAGGA GAATCCCGAA CCTCATCTAG ACCCAGCAGA 
CCAGCGAGCT ATAAGAGAGC AATAGGAGAG GATCATGGTC TGTACTCTAA GGAGCGAGCA 
GAGGAGCAGC CGCGGCCCCC TGTGAAGGCC AACGACTACT CCTCTTCCTC AGAGAGCAGT 
GAAAGCAGCG AAGAGAGTGA AAGTGGAGAA GGAGCAGAGG AGGAAAGCCC TACTGACCGC 
CCCCGTGATG CAGATTCTGA CTCTGTGAAC ACTATGGTTG TCCATGAAGA GGAGGAAGGT 
GAAGGAGGAG AAGAAGAACG TGCTGGAGGA TATGGAGACC AAACCATGCT TGTGCAGAGG 
ACTCCAGAGA AGCGCAGTCA TAATGGATAC ACCAACCTGC CTGATGTGGT TCAGCCTTCT 
CACTCTCCCA CAGACTCGGC CTCCCACTCC TCCCCCGGGA AAGACTCTAC TTATGATTAT 
CAGTCCAGGG GATTGGTCAA GGCCTCTGGA AAATCCTCTT TTACTACCTT TGTGGACCTT 
GGAATGTACC AGCCATCAGG GGGTAGTGGA GATAACATTT CTGTTGGAGG TCTGGGCTCT 
CGTTTTGAGC AGCTAAAGCT GGAGGTGAGG AAGGGCTCCA TGGTCAATGT CAATCCCACA 
AACACACGTC CGGTCAGTGA CACTCCTGAA ATCCGCAAAT ACAAGAAGAG ATTTAACTCT 
GAGATCCTCT GCGCTGCTCT CTGGGGTGTG AATCTGCTGG TCGGGACAGA GAATGGTCTG 
AAGCTGCTGG ACAGAAGCGG TCAGGGTAAA GTGTATCCAC TGATCAACTC GCGCAGATTT 
CAGCAGATGG ATGTCCTGGA GGGACTGAAC CTCCTCATCA CTATATCAGG CAAGAAGAAT 
AAGGTACGCG TGTATTATCT GGCCTGGCTG AGGAATAAGA TCCTCCATAA TGACCCAGAG 
GTGGAGAAGA AACAGGGCTG GACCACTGTA GGGGAAATGG AGGGCTGTGT GCACTATAAA 
GTGGTGAAAT ATGAAAGGAT TAAGTTTCTG GTTATCGCTC TGAAGAATGC AGTGGAGGTT 
TACGCCTGGG CTCCAAAACC TTACCACAAG TTCATGGCCT TCAAGTCATT TGGAGACCTG 
CCGCACAGAC CTGTGTTGGT TGACCTCACA GTGGAAGAGG GTCAGAGGTT AAAGGTCATC 
TATGGCTCCT TGGCTGGCTT CCATGCCATT GATGTTGACT CTGGAAACAA CTACGACATC 
TACATTCCTG TACATATCCA GTCACAGATC ACTCCTCACG CCATCGTGTT CCTGCCCAAC 
TCAGATGGGA TGGAGATGCT GTTGTGTTAT GAGGACGAAG GTGTTTATGT CAACACGTAC 
GGACGCATCA TTAAAGATGT GGTGCTGCAG TGGGGAGAAA TGCCCACATC AGTAGCCCAC 
ATTTGCTCTA ATCAGATTAT GGGTTGGGGA GAGAAAGCCA TTGAGATCCG TTCTGTTGAG 
ACGGGACACC TGGATGGAGT CTTCATGCAC AAGAGAGCCC AGAGGCTTAA GTTCCTGTGT 
GAACGGAACG ACAAGGTGTT TTTCGCGTCA GTGCGTTCCG GTGGCAGCAG CCAAGTTTAC 
TTCATGACCC TCAACAGAAA CTGCATTATG AACTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021331646.1
CDS	1263..5201
Translation	MSENAPTRSLDDIDLAALRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEEEEEEIKAEINMLKKYSHHRNIATYYGAFVKKSPPGHDDQLWLVMEFCGAGSVTDLVKNTKGNSLKEDWIAYICREILRGLSHLHAHKVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDSTYDYRSDIWSLGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPKLKSKKWSKKFIDFIEGCLVKSYPNRPSTEQLLKHSFIRDQPTERQVRIQLKDHIDRTRKKRGEKEETEYEYSGSDEEDENRGDERESSSILNVPGESTLRRDFLRLQQENKERSEALKRQQAQLAAQRRDPEEHKRQLLHDRQKRIEEQKEQRRRLEEQQRKEREMVRQQQDKAPHRRLDDMRREEDRRLAEREQEFIRHKLEEEQRQLEILQQQLLQEQALLMLYNKHCSDSSVNEYKRKQLEEQRQSERLQRQLQQEHAYLVSLQQQQQQQDKKPQMYHYSKNLENNKPAWAREVEERSKLNRQGSPKICTTVSDTNIQSRSESINQSGAAQTPPMQRPVEPQGGQGKMAHLVPLKPYAAPVPRSQSLCDQPTKTMSAFPTQDPSPTPTPRPLHSRELVRQNSDPTSETPAPQSRPMRDDRGPWIRLPEIEQPPKIPQRTASIATALNTNLSSGIRHPVRASNPDLSRNERWERGDSMSIMSNLPQTGSLERHRILSSSKMDSSPTLSHDGRHKPGESRTSSRPSRPASYKRAIGEDHGLYSKERAEEQPRPPVKANDYSSSSESSESSEESESGEGAEEESPTDRPRDADSDSVNTMVVHEEEEGEGGEEERAGGYGDQTMLVQRTPEKRSHNGYTNLPDVVQPSHSPTDSASHSSPGKDSTYDYQSRGLVKASGKSSFTTFVDLGMYQPSGGSGDNISVGGLGSRFEQLKLEVRKGSMVNVNPTNTRPVSDTPEIRKYKKRFNSEILCAALWGVNLLVGTENGLKLLDRSGQGKVYPLINSRRFQQMDVLEGLNLLITISGKKNKVRVYYLAWLRNKILHNDPEVEKKQGWTTVGEMEGCVHYKVVKYERIKFLVIALKNAVEVYAWAPKPYHKFMAFKSFGDLPHRPVLVDLTVEEGQRLKVIYGSLAGFHAIDVDSGNNYDIYIPVHIQSQITPHAIVFLPNSDGMEMLLCYEDEGVYVNTYGRIIKDVVLQWGEMPTSVAHICSNQIMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLNRNCIMNW

Target ORF information:

RefSeq Version	XM_021475971.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio misshapen-like kinase 1 (mink1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021475971.1

ORF Insert Sequence:

ATGTCAGAAA ACGCACCTAC GCGGAGTCTG GATGACATCG ACCTGGCCGC CCTGCGGGAC 
CCAGCAGGGA TCTTCGAGTT GGTGGAGGTC GTGGGCAACG GCACCTATGG ACAAGTTTAC 
AAGGGTCGTC ATGTTAAGAC AGGTCAGCTG GCAGCCATAA AAGTCATGGA TGTGACTGAG 
GAAGAAGAGG AGGAGATTAA AGCGGAGATT AACATGCTGA AGAAGTACAG TCACCATAGA 
AATATCGCCA CCTACTATGG AGCCTTTGTG AAGAAGAGTC CTCCTGGACA CGATGACCAG 
CTCTGGTTGG TGATGGAGTT TTGTGGTGCC GGCTCAGTAA CAGACTTGGT GAAGAACACA 
AAAGGCAACT CTTTAAAAGA AGACTGGATC GCCTATATCT GCAGAGAGAT CCTTAGGGGT 
CTGTCTCACC TCCATGCTCA TAAAGTCATC CACCGAGACA TCAAGGGACA GAATGTGCTG 
CTTACAGAGA ACGCAGAGGT TAAACTGGTG GATTTTGGGG TGAGCGCACA GTTGGATCGT 
ACTGTCGGCA GAAGAAACAC TTTCATTGGC ACTCCATACT GGATGGCCCC AGAAGTCATT 
GCCTGTGATG AAAATCCCGA CTCCACCTAT GATTACAGGA GTGATATCTG GTCTCTGGGA 
ATCACTGCTA TAGAAATGGC TGAAGGAGCA CCTCCGTTGT GTGACATGCA TCCCATGCGA 
GCTTTGTTCC TTATCCCACG GAACCCTCCT CCCAAACTGA AGTCCAAGAA ATGGTCTAAG 
AAGTTCATTG ACTTCATCGA GGGCTGTCTG GTAAAGTCAT ATCCGAATCG TCCGTCCACT 
GAACAGCTCC TGAAACACTC ATTCATCAGA GATCAGCCCA CTGAAAGACA AGTCCGCATC 
CAGCTCAAAG ATCACATCGA CAGAACACGC AAGAAACGTG GCGAAAAAGA GGAGACAGAG 
TACGAGTACA GCGGCAGTGA TGAAGAGGAT GAGAACAGAG GAGATGAGAG AGAATCCAGC 
TCTATTTTGA ATGTTCCTGG TGAGTCGACG TTGCGGAGGG ATTTCCTGCG GCTGCAGCAA 
GAAAATAAAG AGCGTTCAGA GGCCTTAAAG AGACAGCAGG CGCAGCTCGC CGCTCAGCGC 
AGAGACCCTG AGGAGCATAA ACGCCAGCTG CTGCACGACC GGCAGAAGCG CATAGAGGAG 
CAGAAAGAGC AGAGGCGACG CTTAGAGGAG CAACAGAGGA AGGAGAGAGA GATGGTGCGG 
CAACAACAGG ATAAAGCTCC TCACCGGAGA CTCGATGACA TGAGACGAGA GGAGGACAGG 
AGGTTGGCAG AGAGAGAGCA GGAGTTTATA AGACATAAGT TGGAGGAAGA GCAGCGGCAG 
TTAGAGATAC TGCAGCAGCA ATTACTGCAG GAGCAGGCAT TGCTAATGTT GTACAATAAA 
CACTGCTCAG ACTCTTCAGT AAATGAGTAT AAGCGTAAAC AGCTGGAGGA GCAGAGACAG 
TCAGAGAGGC TTCAGAGGCA GTTACAGCAG GAACATGCGT ATCTGGTCTC TCTACAGCAA 
CAGCAGCAGC AGCAAGACAA GAAACCACAG ATGTATCACT ACAGCAAGAA CCTAGAGAAC 
AACAAACCAG CCTGGGCTAG AGAGGTCGAG GAGCGCAGTA AGCTGAACAG ACAGGGCTCT 
CCCAAAATCT GCACCACGGT CTCTGACACT AATATTCAAT CCCGCTCTGA GTCAATCAAC 
CAATCAGGAG CTGCGCAGAC GCCGCCCATG CAGAGACCTG TGGAGCCGCA GGGTGGACAG 
GGAAAGATGG CCCATCTAGT GCCGTTAAAA CCATACGCCG CTCCAGTCCC TCGCTCTCAG 
TCTCTTTGTG ATCAGCCCAC TAAGACCATG TCCGCCTTCC CCACCCAGGA CCCCTCACCC 
ACACCCACAC CTCGTCCTCT ACACTCCAGA GAACTAGTCC GTCAAAACTC TGACCCCACC 
TCAGAGACTC CTGCCCCTCA GTCCCGGCCA ATGAGAGACG ACCGCGGACC CTGGATCCGT 
CTTCCTGAGA TTGAACAACC TCCTAAAATT CCTCAGAGAA CAGCCTCTAT AGCCACTGCA 
CTGAACACAA ATCTCTCCTC TGGTATCAGA CACCCAGTCC GAGCAAGTAA TCCAGATCTG 
AGTCGCAATG AGCGCTGGGA GAGAGGAGAC AGCATGAGCA TCATGTCCAA CCTGCCACAG 
ACCGGCTCTC TGGAGAGACA CCGCATACTC AGCTCATCTA AGATGGATTC CTCTCCAACC 
CTCTCTCATG ATGGCCGACA CAAACCAGGA GAATCCCGAA CCTCATCTAG ACCCAGCAGA 
CCAGCGAGCT ATAAGAGAGC AATAGGAGAG GATCATGGTC TGTACTCTAA GGAGCGAGCA 
GAGGAGCAGC CGCGGCCCCC TGTGAAGGCC AACGACTACT CCTCTTCCTC AGAGAGCAGT 
GAAAGCAGCG AAGAGAGTGA AAGTGGAGAA GGAGCAGAGG AGGAAAGCCC TACTGACCGC 
CCCCGTGATG CAGATTCTGA CTCTGTGAAC ACTATGGTTG TCCATGAAGA GGAGGAAGGT 
GAAGGAGGAG AAGAAGAACG TGCTGGAGGA TATGGAGACC AAACCATGCT TGTGCAGAGG 
ACTCCAGAGA AGCGCAGTCA TAATGGATAC ACCAACCTGC CTGATGTGGT TCAGCCTTCT 
CACTCTCCCA CAGACTCGGC CTCCCACTCC TCCCCCGGGA AAGACTCTAC TTATGATTAT 
CAGTCCAGGG GATTGGTCAA GGCCTCTGGA AAATCCTCTT TTACTACCTT TGTGGACCTT 
GGAATGTACC AGCCATCAGG GGGTAGTGGA GATAACATTT CTGTTGGAGG TCTGGGCTCT 
CGTTTTGAGC AGCTAAAGCT GGAGGTGAGG AAGGGCTCCA TGGTCAATGT CAATCCCACA 
AACACACGTC CGGTCAGTGA CACTCCTGAA ATCCGCAAAT ACAAGAAGAG ATTTAACTCT 
GAGATCCTCT GCGCTGCTCT CTGGGGTGTG AATCTGCTGG TCGGGACAGA GAATGGTCTG 
AAGCTGCTGG ACAGAAGCGG TCAGGGTAAA GTGTATCCAC TGATCAACTC GCGCAGATTT 
CAGCAGATGG ATGTCCTGGA GGGACTGAAC CTCCTCATCA CTATATCAGG CAAGAAGAAT 
AAGGTACGCG TGTATTATCT GGCCTGGCTG AGGAATAAGA TCCTCCATAA TGACCCAGAG 
GTGGAGAAGA AACAGGGCTG GACCACTGTA GGGGAAATGG AGGGCTGTGT GCACTATAAA 
GTGGTGAAAT ATGAAAGGAT TAAGTTTCTG GTTATCGCTC TGAAGAATGC AGTGGAGGTT 
TACGCCTGGG CTCCAAAACC TTACCACAAG TTCATGGCCT TCAAGTCATT TGGAGACCTG 
CCGCACAGAC CTGTGTTGGT TGACCTCACA GTGGAAGAGG GTCAGAGGTT AAAGGTCATC 
TATGGCTCCT TGGCTGGCTT CCATGCCATT GATGTTGACT CTGGAAACAA CTACGACATC 
TACATTCCTG TACATATCCA GTCACAGATC ACTCCTCACG CCATCGTGTT CCTGCCCAAC 
TCAGATGGGA TGGAGATGCT GTTGTGTTAT GAGGACGAAG GTGTTTATGT CAACACGTAC 
GGACGCATCA TTAAAGATGT GGTGCTGCAG TGGGGAGAAA TGCCCACATC AGTAGCCCAC 
ATTTGCTCTA ATCAGATTAT GGGTTGGGGA GAGAAAGCCA TTGAGATCCG TTCTGTTGAG 
ACGGGACACC TGGATGGAGT CTTCATGCAC AAGAGAGCCC AGAGGCTTAA GTTCCTGTGT 
GAACGGAACG ACAAGGTGTT TTTCGCGTCA GTGCGTTCCG GTGGCAGCAG CCAAGTTTAC 
TTCATGACCC TCAACAGAAA CTGCATTATG AACTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mink1 ( XM_021475972.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021331647.1 Danio rerio misshapen-like kinase 1 (mink1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa142549
Clone ID Related Accession (Same CDS sequence)	XM_021475972.1
Accession Version	XM_021475972.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3921bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	misshapen-like kinase 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGAAA ACGCACCTAC GCGGAGTCTG GATGACATCG ACCTGGCCGC CCTGCGGGAC 
CCAGCAGGGA TCTTCGAGTT GGTGGAGGTC GTGGGCAACG GCACCTATGG ACAAGTTTAC 
AAGGGTCGTC ATGTTAAGAC AGGTCAGCTG GCAGCCATAA AAGTCATGGA TGTGACTGAG 
GAAGAAGAGG AGGAGATTAA AGCGGAGATT AACATGCTGA AGAAGTACAG TCACCATAGA 
AATATCGCCA CCTACTATGG AGCCTTTGTG AAGAAGAGTC CTCCTGGACA CGATGACCAG 
CTCTGGTTGG TGATGGAGTT TTGTGGTGCC GGCTCAGTAA CAGACTTGGT GAAGAACACA 
AAAGGCAACT CTTTAAAAGA AGACTGGATC GCCTATATCT GCAGAGAGAT CCTTAGGGGT 
CTGTCTCACC TCCATGCTCA TAAAGTCATC CACCGAGACA TCAAGGGACA GAATGTGCTG 
CTTACAGAGA ACGCAGAGGT TAAACTGGTG GATTTTGGGG TGAGCGCACA GTTGGATCGT 
ACTGTCGGCA GAAGAAACAC TTTCATTGGC ACTCCATACT GGATGGCCCC AGAAGTCATT 
GCCTGTGATG AAAATCCCGA CTCCACCTAT GATTACAGGA GTGATATCTG GTCTCTGGGA 
ATCACTGCTA TAGAAATGGC TGAAGGAGCA CCTCCGTTGT GTGACATGCA TCCCATGCGA 
GCTTTGTTCC TTATCCCACG GAACCCTCCT CCCAAACTGA AGTCCAAGAA ATGGTCTAAG 
AAGTTCATTG ACTTCATCGA GGGCTGTCTG GTAAAGTCAT ATCCGAATCG TCCGTCCACT 
GAACAGCTCC TGAAACACTC ATTCATCAGA GATCAGCCCA CTGAAAGACA AGTCCGCATC 
CAGCTCAAAG ATCACATCGA CAGAACACGC AAGAAACGTG GCGAAAAAGA GGAGACAGAG 
TACGAGTACA GCGGCAGTGA TGAAGAGGAT GAGAACAGAG GAGATGAGAG AGAATCCAGC 
TCTATTTTGA ATGTTCCTGG TGAGTCGACG TTGCGGAGGG ATTTCCTGCG GCTGCAGCAA 
GAAAATAAAG AGCGTTCAGA GGCCTTAAAG AGACAGCAGG CGCAGCTCGC CGCTCAGCGC 
AGAGACCCTG AGGAGCATAA ACGCCAGCTG CTGCACGACC GGCAGAAGCG CATAGAGGAG 
CAGAAAGAGC AGAGGCGACG CTTAGAGGAG CAACAGAGGA AGGAGAGAGA GATGGTGCGG 
CAACAACAGG ATAAAGCTCC TCACCGGAGA CTCGATGACA TGAGACGAGA GGAGGACAGG 
AGGTTGGCAG AGAGAGAGCA GGAGTTTATA AGACATAAGT TGGAGGAAGA GCAGCGGCAG 
TTAGAGATAC TGCAGCAGCA ATTACTGCAG GAGCAGGCAT TGCTAATGTT GTACAATAAA 
CACTGCTCAG ACTCTTCAGT AAATGAGTAT AAGCGTAAAC AGCTGGAGGA GCAGAGACAG 
TCAGAGAGGC TTCAGAGGCA GTTACAGCAG GAACATGCGT ATCTGGTCTC TCTACAGCAA 
CAGCAGCAGC AGCAAGACAA GAAACCACAG ATGTATCACT ACAGCAAGAA CCTAGAGAAC 
AACAAACCAG CCTGGGCTAG AGAGGTCGAG GAGCGCAGTA AGCTGAACAG ACAGGGCTCT 
CCCAAAATCT GCACCACGGT CTCTGACACT AATATTCAAT CCCGCTCTGA GTCAATCAAC 
CAATCAGGAG CTGCGCAGAC GCCGCCCATG CAGAGACCTG TGGAGCCGCA GGGTGGACAG 
GGAAAGTTCC AGATGGCCCA TCTAGTGCCG TTAAAACCAT ACGCCGCTCC AGTCCCTCGC 
TCTCAGTCTC TTTGTGATCA GCCCACTAAG ACCATGTCCG CCTTCCCCAC CCAGGACCCC 
TCACCCACAC CCACACCTCG TCCTCTACAC TCCAGAGAAC TAGTCCGTCA AAACTCTGAC 
CCCACCTCAG AGACTCCTGC CCCTCAGTCC CGGCCAATGA GAGACGACCG CGGACCCTGG 
ATCCGTCTTC CTGAGATTGA ACAACCTCCT AAAATTCCTC AGAGAACAGC CTCTATAGCC 
ACTGCACTGA ACACAAATCT CTCCTCTGGT ATCAGACACC CAGTCCGAGC AAGTAATCCA 
GATCTGAGTC GCAATGAGCG CTGGGAGAGA GGAGACAGCA TGAGCATCAT GTCCAACCTG 
CCACAGACCG GCTCTCTGGA GAGACACCGC ATACTCAGCT CATCTAAGAT GGATTCCTCT 
CCAACCCTCT CTCATGATGG CCGACACAAA CCAGGAGAAT CCCGAACCTC ATCTAGACCC 
AGCAGACCAG CGGATCATGG TCTGTACTCT AAGGAGCGAG CAGAGGAGCA GCCGCGGCCC 
CCTGTGAAGG CCAACGACTA CTCCTCTTCC TCAGAGAGCA GTGAAAGCAG CGAAGAGAGT 
GAAAGTGGAG AAGGAGCAGA GGAGGAAAGC CCTACTGACC GCCCCCGTGA TGCAGATTCT 
GACTCTGTGA ACACTATGGT TGTCCATGAA GAGGAGGAAG GTGAAGGAGG AGAAGAAGAA 
CGTGCTGGAG GATATGGAGA CCAAACCATG CTTGTGCAGA GGACTCCAGA GAAGCGCAGT 
CATAATGGAT ACACCAACCT GCCTGATGTG GTTCAGCCTT CTCACTCTCC CACAGACTCG 
GCCTCCCACT CCTCCCCCGG GAAAGACTCT ACTTATGATT ATCAGTCCAG GGGATTGGTC 
AAGGCCTCTG GAAAATCCTC TTTTACTACC TTTGTGGACC TTGGAATGTA CCAGCCATCA 
GGGGGTAGTG GAGATAACAT TTCTGTTGGA GGTCTGGGCT CTCGTTTTGA GCAGCTAAAG 
CTGGAGGTGA GGAAGGGCTC CATGGTCAAT GTCAATCCCA CAAACACACG TCCGGTCAGT 
GACACTCCTG AAATCCGCAA ATACAAGAAG AGATTTAACT CTGAGATCCT CTGCGCTGCT 
CTCTGGGGTG TGAATCTGCT GGTCGGGACA GAGAATGGTC TGAAGCTGCT GGACAGAAGC 
GGTCAGGGTA AAGTGTATCC ACTGATCAAC TCGCGCAGAT TTCAGCAGAT GGATGTCCTG 
GAGGGACTGA ACCTCCTCAT CACTATATCA GGCAAGAAGA ATAAGGTACG CGTGTATTAT 
CTGGCCTGGC TGAGGAATAA GATCCTCCAT AATGACCCAG AGGTGGAGAA GAAACAGGGC 
TGGACCACTG TAGGGGAAAT GGAGGGCTGT GTGCACTATA AAGTGGTGAA ATATGAAAGG 
ATTAAGTTTC TGGTTATCGC TCTGAAGAAT GCAGTGGAGG TTTACGCCTG GGCTCCAAAA 
CCTTACCACA AGTTCATGGC CTTCAAGTCA TTTGGAGACC TGCCGCACAG ACCTGTGTTG 
GTTGACCTCA CAGTGGAAGA GGGTCAGAGG TTAAAGGTCA TCTATGGCTC CTTGGCTGGC 
TTCCATGCCA TTGATGTTGA CTCTGGAAAC AACTACGACA TCTACATTCC TGTACATATC 
CAGTCACAGA TCACTCCTCA CGCCATCGTG TTCCTGCCCA ACTCAGATGG GATGGAGATG 
CTGTTGTGTT ATGAGGACGA AGGTGTTTAT GTCAACACGT ACGGACGCAT CATTAAAGAT 
GTGGTGCTGC AGTGGGGAGA AATGCCCACA TCAGTAGCCC ACATTTGCTC TAATCAGATT 
ATGGGTTGGG GAGAGAAAGC CATTGAGATC CGTTCTGTTG AGACGGGACA CCTGGATGGA 
GTCTTCATGC ACAAGAGAGC CCAGAGGCTT AAGTTCCTGT GTGAACGGAA CGACAAGGTG 
TTTTTCGCGT CAGTGCGTTC CGGTGGCAGC AGCCAAGTTT ACTTCATGAC CCTCAACAGA 
AACTGCATTA TGAACTGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021331647.1
CDS	1261..5181
Translation	MSENAPTRSLDDIDLAALRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEEEEEEIKAEINMLKKYSHHRNIATYYGAFVKKSPPGHDDQLWLVMEFCGAGSVTDLVKNTKGNSLKEDWIAYICREILRGLSHLHAHKVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDSTYDYRSDIWSLGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPKLKSKKWSKKFIDFIEGCLVKSYPNRPSTEQLLKHSFIRDQPTERQVRIQLKDHIDRTRKKRGEKEETEYEYSGSDEEDENRGDERESSSILNVPGESTLRRDFLRLQQENKERSEALKRQQAQLAAQRRDPEEHKRQLLHDRQKRIEEQKEQRRRLEEQQRKEREMVRQQQDKAPHRRLDDMRREEDRRLAEREQEFIRHKLEEEQRQLEILQQQLLQEQALLMLYNKHCSDSSVNEYKRKQLEEQRQSERLQRQLQQEHAYLVSLQQQQQQQDKKPQMYHYSKNLENNKPAWAREVEERSKLNRQGSPKICTTVSDTNIQSRSESINQSGAAQTPPMQRPVEPQGGQGKFQMAHLVPLKPYAAPVPRSQSLCDQPTKTMSAFPTQDPSPTPTPRPLHSRELVRQNSDPTSETPAPQSRPMRDDRGPWIRLPEIEQPPKIPQRTASIATALNTNLSSGIRHPVRASNPDLSRNERWERGDSMSIMSNLPQTGSLERHRILSSSKMDSSPTLSHDGRHKPGESRTSSRPSRPADHGLYSKERAEEQPRPPVKANDYSSSSESSESSEESESGEGAEEESPTDRPRDADSDSVNTMVVHEEEEGEGGEEERAGGYGDQTMLVQRTPEKRSHNGYTNLPDVVQPSHSPTDSASHSSPGKDSTYDYQSRGLVKASGKSSFTTFVDLGMYQPSGGSGDNISVGGLGSRFEQLKLEVRKGSMVNVNPTNTRPVSDTPEIRKYKKRFNSEILCAALWGVNLLVGTENGLKLLDRSGQGKVYPLINSRRFQQMDVLEGLNLLITISGKKNKVRVYYLAWLRNKILHNDPEVEKKQGWTTVGEMEGCVHYKVVKYERIKFLVIALKNAVEVYAWAPKPYHKFMAFKSFGDLPHRPVLVDLTVEEGQRLKVIYGSLAGFHAIDVDSGNNYDIYIPVHIQSQITPHAIVFLPNSDGMEMLLCYEDEGVYVNTYGRIIKDVVLQWGEMPTSVAHICSNQIMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLNRNCIMNW

Target ORF information:

RefSeq Version	XM_021475972.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio misshapen-like kinase 1 (mink1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021475972.1

ORF Insert Sequence:

ATGTCAGAAA ACGCACCTAC GCGGAGTCTG GATGACATCG ACCTGGCCGC CCTGCGGGAC 
CCAGCAGGGA TCTTCGAGTT GGTGGAGGTC GTGGGCAACG GCACCTATGG ACAAGTTTAC 
AAGGGTCGTC ATGTTAAGAC AGGTCAGCTG GCAGCCATAA AAGTCATGGA TGTGACTGAG 
GAAGAAGAGG AGGAGATTAA AGCGGAGATT AACATGCTGA AGAAGTACAG TCACCATAGA 
AATATCGCCA CCTACTATGG AGCCTTTGTG AAGAAGAGTC CTCCTGGACA CGATGACCAG 
CTCTGGTTGG TGATGGAGTT TTGTGGTGCC GGCTCAGTAA CAGACTTGGT GAAGAACACA 
AAAGGCAACT CTTTAAAAGA AGACTGGATC GCCTATATCT GCAGAGAGAT CCTTAGGGGT 
CTGTCTCACC TCCATGCTCA TAAAGTCATC CACCGAGACA TCAAGGGACA GAATGTGCTG 
CTTACAGAGA ACGCAGAGGT TAAACTGGTG GATTTTGGGG TGAGCGCACA GTTGGATCGT 
ACTGTCGGCA GAAGAAACAC TTTCATTGGC ACTCCATACT GGATGGCCCC AGAAGTCATT 
GCCTGTGATG AAAATCCCGA CTCCACCTAT GATTACAGGA GTGATATCTG GTCTCTGGGA 
ATCACTGCTA TAGAAATGGC TGAAGGAGCA CCTCCGTTGT GTGACATGCA TCCCATGCGA 
GCTTTGTTCC TTATCCCACG GAACCCTCCT CCCAAACTGA AGTCCAAGAA ATGGTCTAAG 
AAGTTCATTG ACTTCATCGA GGGCTGTCTG GTAAAGTCAT ATCCGAATCG TCCGTCCACT 
GAACAGCTCC TGAAACACTC ATTCATCAGA GATCAGCCCA CTGAAAGACA AGTCCGCATC 
CAGCTCAAAG ATCACATCGA CAGAACACGC AAGAAACGTG GCGAAAAAGA GGAGACAGAG 
TACGAGTACA GCGGCAGTGA TGAAGAGGAT GAGAACAGAG GAGATGAGAG AGAATCCAGC 
TCTATTTTGA ATGTTCCTGG TGAGTCGACG TTGCGGAGGG ATTTCCTGCG GCTGCAGCAA 
GAAAATAAAG AGCGTTCAGA GGCCTTAAAG AGACAGCAGG CGCAGCTCGC CGCTCAGCGC 
AGAGACCCTG AGGAGCATAA ACGCCAGCTG CTGCACGACC GGCAGAAGCG CATAGAGGAG 
CAGAAAGAGC AGAGGCGACG CTTAGAGGAG CAACAGAGGA AGGAGAGAGA GATGGTGCGG 
CAACAACAGG ATAAAGCTCC TCACCGGAGA CTCGATGACA TGAGACGAGA GGAGGACAGG 
AGGTTGGCAG AGAGAGAGCA GGAGTTTATA AGACATAAGT TGGAGGAAGA GCAGCGGCAG 
TTAGAGATAC TGCAGCAGCA ATTACTGCAG GAGCAGGCAT TGCTAATGTT GTACAATAAA 
CACTGCTCAG ACTCTTCAGT AAATGAGTAT AAGCGTAAAC AGCTGGAGGA GCAGAGACAG 
TCAGAGAGGC TTCAGAGGCA GTTACAGCAG GAACATGCGT ATCTGGTCTC TCTACAGCAA 
CAGCAGCAGC AGCAAGACAA GAAACCACAG ATGTATCACT ACAGCAAGAA CCTAGAGAAC 
AACAAACCAG CCTGGGCTAG AGAGGTCGAG GAGCGCAGTA AGCTGAACAG ACAGGGCTCT 
CCCAAAATCT GCACCACGGT CTCTGACACT AATATTCAAT CCCGCTCTGA GTCAATCAAC 
CAATCAGGAG CTGCGCAGAC GCCGCCCATG CAGAGACCTG TGGAGCCGCA GGGTGGACAG 
GGAAAGTTCC AGATGGCCCA TCTAGTGCCG TTAAAACCAT ACGCCGCTCC AGTCCCTCGC 
TCTCAGTCTC TTTGTGATCA GCCCACTAAG ACCATGTCCG CCTTCCCCAC CCAGGACCCC 
TCACCCACAC CCACACCTCG TCCTCTACAC TCCAGAGAAC TAGTCCGTCA AAACTCTGAC 
CCCACCTCAG AGACTCCTGC CCCTCAGTCC CGGCCAATGA GAGACGACCG CGGACCCTGG 
ATCCGTCTTC CTGAGATTGA ACAACCTCCT AAAATTCCTC AGAGAACAGC CTCTATAGCC 
ACTGCACTGA ACACAAATCT CTCCTCTGGT ATCAGACACC CAGTCCGAGC AAGTAATCCA 
GATCTGAGTC GCAATGAGCG CTGGGAGAGA GGAGACAGCA TGAGCATCAT GTCCAACCTG 
CCACAGACCG GCTCTCTGGA GAGACACCGC ATACTCAGCT CATCTAAGAT GGATTCCTCT 
CCAACCCTCT CTCATGATGG CCGACACAAA CCAGGAGAAT CCCGAACCTC ATCTAGACCC 
AGCAGACCAG CGGATCATGG TCTGTACTCT AAGGAGCGAG CAGAGGAGCA GCCGCGGCCC 
CCTGTGAAGG CCAACGACTA CTCCTCTTCC TCAGAGAGCA GTGAAAGCAG CGAAGAGAGT 
GAAAGTGGAG AAGGAGCAGA GGAGGAAAGC CCTACTGACC GCCCCCGTGA TGCAGATTCT 
GACTCTGTGA ACACTATGGT TGTCCATGAA GAGGAGGAAG GTGAAGGAGG AGAAGAAGAA 
CGTGCTGGAG GATATGGAGA CCAAACCATG CTTGTGCAGA GGACTCCAGA GAAGCGCAGT 
CATAATGGAT ACACCAACCT GCCTGATGTG GTTCAGCCTT CTCACTCTCC CACAGACTCG 
GCCTCCCACT CCTCCCCCGG GAAAGACTCT ACTTATGATT ATCAGTCCAG GGGATTGGTC 
AAGGCCTCTG GAAAATCCTC TTTTACTACC TTTGTGGACC TTGGAATGTA CCAGCCATCA 
GGGGGTAGTG GAGATAACAT TTCTGTTGGA GGTCTGGGCT CTCGTTTTGA GCAGCTAAAG 
CTGGAGGTGA GGAAGGGCTC CATGGTCAAT GTCAATCCCA CAAACACACG TCCGGTCAGT 
GACACTCCTG AAATCCGCAA ATACAAGAAG AGATTTAACT CTGAGATCCT CTGCGCTGCT 
CTCTGGGGTG TGAATCTGCT GGTCGGGACA GAGAATGGTC TGAAGCTGCT GGACAGAAGC 
GGTCAGGGTA AAGTGTATCC ACTGATCAAC TCGCGCAGAT TTCAGCAGAT GGATGTCCTG 
GAGGGACTGA ACCTCCTCAT CACTATATCA GGCAAGAAGA ATAAGGTACG CGTGTATTAT 
CTGGCCTGGC TGAGGAATAA GATCCTCCAT AATGACCCAG AGGTGGAGAA GAAACAGGGC 
TGGACCACTG TAGGGGAAAT GGAGGGCTGT GTGCACTATA AAGTGGTGAA ATATGAAAGG 
ATTAAGTTTC TGGTTATCGC TCTGAAGAAT GCAGTGGAGG TTTACGCCTG GGCTCCAAAA 
CCTTACCACA AGTTCATGGC CTTCAAGTCA TTTGGAGACC TGCCGCACAG ACCTGTGTTG 
GTTGACCTCA CAGTGGAAGA GGGTCAGAGG TTAAAGGTCA TCTATGGCTC CTTGGCTGGC 
TTCCATGCCA TTGATGTTGA CTCTGGAAAC AACTACGACA TCTACATTCC TGTACATATC 
CAGTCACAGA TCACTCCTCA CGCCATCGTG TTCCTGCCCA ACTCAGATGG GATGGAGATG 
CTGTTGTGTT ATGAGGACGA AGGTGTTTAT GTCAACACGT ACGGACGCAT CATTAAAGAT 
GTGGTGCTGC AGTGGGGAGA AATGCCCACA TCAGTAGCCC ACATTTGCTC TAATCAGATT 
ATGGGTTGGG GAGAGAAAGC CATTGAGATC CGTTCTGTTG AGACGGGACA CCTGGATGGA 
GTCTTCATGC ACAAGAGAGC CCAGAGGCTT AAGTTCCTGT GTGAACGGAA CGACAAGGTG 
TTTTTCGCGT CAGTGCGTTC CGGTGGCAGC AGCCAAGTTT ACTTCATGAC CCTCAACAGA 
AACTGCATTA TGAACTGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mink1 ( XM_021475973.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021331648.1 Danio rerio misshapen-like kinase 1 (mink1), transcript variant X6, mRNA.

Price & Availability↑

CloneID	ODa142550
Clone ID Related Accession (Same CDS sequence)	XM_021475973.1
Accession Version	XM_021475973.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3837bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	misshapen-like kinase 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGAAA ACGCACCTAC GCGGAGTCTG GATGACATCG ACCTGGCCGC CCTGCGGGAC 
CCAGCAGGGA TCTTCGAGTT GGTGGAGGTC GTGGGCAACG GCACCTATGG ACAAGTTTAC 
AAGGGTCGTC ATGTTAAGAC AGGTCAGCTG GCAGCCATAA AAGTCATGGA TGTGACTGAG 
GAAGAAGAGG AGGAGATTAA AGCGGAGATT AACATGCTGA AGAAGTACAG TCACCATAGA 
AATATCGCCA CCTACTATGG AGCCTTTGTG AAGAAGAGTC CTCCTGGACA CGATGACCAG 
CTCTGGTTGG TGATGGAGTT TTGTGGTGCC GGCTCAGTAA CAGACTTGGT GAAGAACACA 
AAAGGCAACT CTTTAAAAGA AGACTGGATC GCCTATATCT GCAGAGAGAT CCTTAGGGGT 
CTGTCTCACC TCCATGCTCA TAAAGTCATC CACCGAGACA TCAAGGGACA GAATGTGCTG 
CTTACAGAGA ACGCAGAGGT TAAACTGGTG GATTTTGGGG TGAGCGCACA GTTGGATCGT 
ACTGTCGGCA GAAGAAACAC TTTCATTGGC ACTCCATACT GGATGGCCCC AGAAGTCATT 
GCCTGTGATG AAAATCCCGA CTCCACCTAT GATTACAGGA GTGATATCTG GTCTCTGGGA 
ATCACTGCTA TAGAAATGGC TGAAGGAGCA CCTCCGTTGT GTGACATGCA TCCCATGCGA 
GCTTTGTTCC TTATCCCACG GAACCCTCCT CCCAAACTGA AGTCCAAGAA ATGGTCTAAG 
AAGTTCATTG ACTTCATCGA GGGCTGTCTG GTAAAGTCAT ATCCGAATCG TCCGTCCACT 
GAACAGCTCC TGAAACACTC ATTCATCAGA GATCAGCCCA CTGAAAGACA AGTCCGCATC 
CAGCTCAAAG ATCACATCGA CAGAACACGC AAGAAACGTG GCGAAAAAGA GGAGACAGAG 
TACGAGTACA GCGGCAGTGA TGAAGAGGAT GAGAACAGAG GAGATGAGAG AGAATCCAGC 
TCTATTTTGA ATGTTCCTGG TGAGTCGACG TTGCGGAGGG ATTTCCTGCG GCTGCAGCAA 
GAAAATAAAG AGCGTTCAGA GGCCTTAAAG AGACAGCAGG CGCAGCTCGC CGCTCAGCGC 
AGAGACCCTG AGGAGCATAA ACGCCAGCTG CTGCACGACC GGCAGAAGCG CATAGAGGAG 
CAGAAAGAGC AGAGGCGACG CTTAGAGGAG CAACAGAGGA AGGAGAGAGA GATGGTGCGG 
CAACAACAGG ATAAAGCTCC TCACCGGAGA CTCGATGACA TGAGACGAGA GGAGGACAGG 
AGGTTGGCAG AGAGAGAGCA GGAGTTTATA AGACATAAGT TGGAGGAAGA GCAGCGGCAG 
TTAGAGATAC TGCAGCAGCA ATTACTGCAG GAGCAGGCAT TGCTAATGTT GTACAATAAA 
CACTGCTCAG ACTCTTCAGT AAATGAGTAT AAGCGTAAAC AGCTGGAGGA GCAGAGACAG 
TCAGAGAGGC TTCAGAGGCA GTTACAGCAG GAACATGCGT ATCTGGTCTC TCTACAGCAA 
CAGCAGCAGC AGCAAGACAA GAAACCACAG ATGTATCACT ACAGCAAGAA CCTAGAGAAC 
AACAAACCAG CCTGGGCTAG AGAGGTCGAG GAGCGCAGTA AGCTGAACAG ACAGGGCTCT 
CCCAAAATCT GCACCACGGT CTCTGACACT AATATTCAAT CCCGCTCTGA GTCAATCAAC 
CAATCAGGAG CTGCGCAGAC GCCGCCCATG CAGAGACCTG TGGAGCCGCA GGGTGGACAG 
GGAAAGGACC CCTCACCCAC ACCCACACCT CGTCCTCTAC ACTCCAGAGA ACTAGTCCGT 
CAAAACTCTG ACCCCACCTC AGAGACTCCT GCCCCTCAGT CCCGGCCAAT GAGAGACGAC 
CGCGGACCCT GGATCCGTCT TCCTGAGATT GAACAACCTC CTAAAATTCC TCAGAGAACA 
GCCTCTATAG CCACTGCACT GAACACAAAT CTCTCCTCTG GTATCAGACA CCCAGTCCGA 
GCAAGTAATC CAGATCTGAG TCGCAATGAG CGCTGGGAGA GAGGAGACAG CATGAGCATC 
ATGTCCAACC TGCCACAGAC CGGCTCTCTG GAGAGACACC GCATACTCAG CTCATCTAAG 
ATGGATTCCT CTCCAACCCT CTCTCATGAT GGCCGACACA AACCAGGAGA ATCCCGAACC 
TCATCTAGAC CCAGCAGACC AGCGAGCTAT AAGAGAGCAA TAGGAGAGGA TCATGGTCTG 
TACTCTAAGG AGCGAGCAGA GGAGCAGCCG CGGCCCCCTG TGAAGGCCAA CGACTACTCC 
TCTTCCTCAG AGAGCAGTGA AAGCAGCGAA GAGAGTGAAA GTGGAGAAGG AGCAGAGGAG 
GAAAGCCCTA CTGACCGCCC CCGTGATGCA GATTCTGACT CTGTGAACAC TATGGTTGTC 
CATGAAGAGG AGGAAGGTGA AGGAGGAGAA GAAGAACGTG CTGGAGGATA TGGAGACCAA 
ACCATGCTTG TGCAGAGGAC TCCAGAGAAG CGCAGTCATA ATGGATACAC CAACCTGCCT 
GATGTGGTTC AGCCTTCTCA CTCTCCCACA GACTCGGCCT CCCACTCCTC CCCCGGGAAA 
GACTCTACTT ATGATTATCA GTCCAGGGGA TTGGTCAAGG CCTCTGGAAA ATCCTCTTTT 
ACTACCTTTG TGGACCTTGG AATGTACCAG CCATCAGGGG GTAGTGGAGA TAACATTTCT 
GTTGGAGGTC TGGGCTCTCG TTTTGAGCAG CTAAAGCTGG AGGTGAGGAA GGGCTCCATG 
GTCAATGTCA ATCCCACAAA CACACGTCCG GTCAGTGACA CTCCTGAAAT CCGCAAATAC 
AAGAAGAGAT TTAACTCTGA GATCCTCTGC GCTGCTCTCT GGGGTGTGAA TCTGCTGGTC 
GGGACAGAGA ATGGTCTGAA GCTGCTGGAC AGAAGCGGTC AGGGTAAAGT GTATCCACTG 
ATCAACTCGC GCAGATTTCA GCAGATGGAT GTCCTGGAGG GACTGAACCT CCTCATCACT 
ATATCAGGCA AGAAGAATAA GGTACGCGTG TATTATCTGG CCTGGCTGAG GAATAAGATC 
CTCCATAATG ACCCAGAGGT GGAGAAGAAA CAGGGCTGGA CCACTGTAGG GGAAATGGAG 
GGCTGTGTGC ACTATAAAGT GGTGAAATAT GAAAGGATTA AGTTTCTGGT TATCGCTCTG 
AAGAATGCAG TGGAGGTTTA CGCCTGGGCT CCAAAACCTT ACCACAAGTT CATGGCCTTC 
AAGTCATTTG GAGACCTGCC GCACAGACCT GTGTTGGTTG ACCTCACAGT GGAAGAGGGT 
CAGAGGTTAA AGGTCATCTA TGGCTCCTTG GCTGGCTTCC ATGCCATTGA TGTTGACTCT 
GGAAACAACT ACGACATCTA CATTCCTGTA CATATCCAGT CACAGATCAC TCCTCACGCC 
ATCGTGTTCC TGCCCAACTC AGATGGGATG GAGATGCTGT TGTGTTATGA GGACGAAGGT 
GTTTATGTCA ACACGTACGG ACGCATCATT AAAGATGTGG TGCTGCAGTG GGGAGAAATG 
CCCACATCAG TAGCCCACAT TTGCTCTAAT CAGATTATGG GTTGGGGAGA GAAAGCCATT 
GAGATCCGTT CTGTTGAGAC GGGACACCTG GATGGAGTCT TCATGCACAA GAGAGCCCAG 
AGGCTTAAGT TCCTGTGTGA ACGGAACGAC AAGGTGTTTT TCGCGTCAGT GCGTTCCGGT 
GGCAGCAGCC AAGTTTACTT CATGACCCTC AACAGAAACT GCATTATGAA CTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021331648.1
CDS	1261..5097
Translation	MSENAPTRSLDDIDLAALRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEEEEEEIKAEINMLKKYSHHRNIATYYGAFVKKSPPGHDDQLWLVMEFCGAGSVTDLVKNTKGNSLKEDWIAYICREILRGLSHLHAHKVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDSTYDYRSDIWSLGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPKLKSKKWSKKFIDFIEGCLVKSYPNRPSTEQLLKHSFIRDQPTERQVRIQLKDHIDRTRKKRGEKEETEYEYSGSDEEDENRGDERESSSILNVPGESTLRRDFLRLQQENKERSEALKRQQAQLAAQRRDPEEHKRQLLHDRQKRIEEQKEQRRRLEEQQRKEREMVRQQQDKAPHRRLDDMRREEDRRLAEREQEFIRHKLEEEQRQLEILQQQLLQEQALLMLYNKHCSDSSVNEYKRKQLEEQRQSERLQRQLQQEHAYLVSLQQQQQQQDKKPQMYHYSKNLENNKPAWAREVEERSKLNRQGSPKICTTVSDTNIQSRSESINQSGAAQTPPMQRPVEPQGGQGKDPSPTPTPRPLHSRELVRQNSDPTSETPAPQSRPMRDDRGPWIRLPEIEQPPKIPQRTASIATALNTNLSSGIRHPVRASNPDLSRNERWERGDSMSIMSNLPQTGSLERHRILSSSKMDSSPTLSHDGRHKPGESRTSSRPSRPASYKRAIGEDHGLYSKERAEEQPRPPVKANDYSSSSESSESSEESESGEGAEEESPTDRPRDADSDSVNTMVVHEEEEGEGGEEERAGGYGDQTMLVQRTPEKRSHNGYTNLPDVVQPSHSPTDSASHSSPGKDSTYDYQSRGLVKASGKSSFTTFVDLGMYQPSGGSGDNISVGGLGSRFEQLKLEVRKGSMVNVNPTNTRPVSDTPEIRKYKKRFNSEILCAALWGVNLLVGTENGLKLLDRSGQGKVYPLINSRRFQQMDVLEGLNLLITISGKKNKVRVYYLAWLRNKILHNDPEVEKKQGWTTVGEMEGCVHYKVVKYERIKFLVIALKNAVEVYAWAPKPYHKFMAFKSFGDLPHRPVLVDLTVEEGQRLKVIYGSLAGFHAIDVDSGNNYDIYIPVHIQSQITPHAIVFLPNSDGMEMLLCYEDEGVYVNTYGRIIKDVVLQWGEMPTSVAHICSNQIMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLNRNCIMNW

Target ORF information:

RefSeq Version	XM_021475973.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio misshapen-like kinase 1 (mink1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021475973.1

ORF Insert Sequence:

ATGTCAGAAA ACGCACCTAC GCGGAGTCTG GATGACATCG ACCTGGCCGC CCTGCGGGAC 
CCAGCAGGGA TCTTCGAGTT GGTGGAGGTC GTGGGCAACG GCACCTATGG ACAAGTTTAC 
AAGGGTCGTC ATGTTAAGAC AGGTCAGCTG GCAGCCATAA AAGTCATGGA TGTGACTGAG 
GAAGAAGAGG AGGAGATTAA AGCGGAGATT AACATGCTGA AGAAGTACAG TCACCATAGA 
AATATCGCCA CCTACTATGG AGCCTTTGTG AAGAAGAGTC CTCCTGGACA CGATGACCAG 
CTCTGGTTGG TGATGGAGTT TTGTGGTGCC GGCTCAGTAA CAGACTTGGT GAAGAACACA 
AAAGGCAACT CTTTAAAAGA AGACTGGATC GCCTATATCT GCAGAGAGAT CCTTAGGGGT 
CTGTCTCACC TCCATGCTCA TAAAGTCATC CACCGAGACA TCAAGGGACA GAATGTGCTG 
CTTACAGAGA ACGCAGAGGT TAAACTGGTG GATTTTGGGG TGAGCGCACA GTTGGATCGT 
ACTGTCGGCA GAAGAAACAC TTTCATTGGC ACTCCATACT GGATGGCCCC AGAAGTCATT 
GCCTGTGATG AAAATCCCGA CTCCACCTAT GATTACAGGA GTGATATCTG GTCTCTGGGA 
ATCACTGCTA TAGAAATGGC TGAAGGAGCA CCTCCGTTGT GTGACATGCA TCCCATGCGA 
GCTTTGTTCC TTATCCCACG GAACCCTCCT CCCAAACTGA AGTCCAAGAA ATGGTCTAAG 
AAGTTCATTG ACTTCATCGA GGGCTGTCTG GTAAAGTCAT ATCCGAATCG TCCGTCCACT 
GAACAGCTCC TGAAACACTC ATTCATCAGA GATCAGCCCA CTGAAAGACA AGTCCGCATC 
CAGCTCAAAG ATCACATCGA CAGAACACGC AAGAAACGTG GCGAAAAAGA GGAGACAGAG 
TACGAGTACA GCGGCAGTGA TGAAGAGGAT GAGAACAGAG GAGATGAGAG AGAATCCAGC 
TCTATTTTGA ATGTTCCTGG TGAGTCGACG TTGCGGAGGG ATTTCCTGCG GCTGCAGCAA 
GAAAATAAAG AGCGTTCAGA GGCCTTAAAG AGACAGCAGG CGCAGCTCGC CGCTCAGCGC 
AGAGACCCTG AGGAGCATAA ACGCCAGCTG CTGCACGACC GGCAGAAGCG CATAGAGGAG 
CAGAAAGAGC AGAGGCGACG CTTAGAGGAG CAACAGAGGA AGGAGAGAGA GATGGTGCGG 
CAACAACAGG ATAAAGCTCC TCACCGGAGA CTCGATGACA TGAGACGAGA GGAGGACAGG 
AGGTTGGCAG AGAGAGAGCA GGAGTTTATA AGACATAAGT TGGAGGAAGA GCAGCGGCAG 
TTAGAGATAC TGCAGCAGCA ATTACTGCAG GAGCAGGCAT TGCTAATGTT GTACAATAAA 
CACTGCTCAG ACTCTTCAGT AAATGAGTAT AAGCGTAAAC AGCTGGAGGA GCAGAGACAG 
TCAGAGAGGC TTCAGAGGCA GTTACAGCAG GAACATGCGT ATCTGGTCTC TCTACAGCAA 
CAGCAGCAGC AGCAAGACAA GAAACCACAG ATGTATCACT ACAGCAAGAA CCTAGAGAAC 
AACAAACCAG CCTGGGCTAG AGAGGTCGAG GAGCGCAGTA AGCTGAACAG ACAGGGCTCT 
CCCAAAATCT GCACCACGGT CTCTGACACT AATATTCAAT CCCGCTCTGA GTCAATCAAC 
CAATCAGGAG CTGCGCAGAC GCCGCCCATG CAGAGACCTG TGGAGCCGCA GGGTGGACAG 
GGAAAGGACC CCTCACCCAC ACCCACACCT CGTCCTCTAC ACTCCAGAGA ACTAGTCCGT 
CAAAACTCTG ACCCCACCTC AGAGACTCCT GCCCCTCAGT CCCGGCCAAT GAGAGACGAC 
CGCGGACCCT GGATCCGTCT TCCTGAGATT GAACAACCTC CTAAAATTCC TCAGAGAACA 
GCCTCTATAG CCACTGCACT GAACACAAAT CTCTCCTCTG GTATCAGACA CCCAGTCCGA 
GCAAGTAATC CAGATCTGAG TCGCAATGAG CGCTGGGAGA GAGGAGACAG CATGAGCATC 
ATGTCCAACC TGCCACAGAC CGGCTCTCTG GAGAGACACC GCATACTCAG CTCATCTAAG 
ATGGATTCCT CTCCAACCCT CTCTCATGAT GGCCGACACA AACCAGGAGA ATCCCGAACC 
TCATCTAGAC CCAGCAGACC AGCGAGCTAT AAGAGAGCAA TAGGAGAGGA TCATGGTCTG 
TACTCTAAGG AGCGAGCAGA GGAGCAGCCG CGGCCCCCTG TGAAGGCCAA CGACTACTCC 
TCTTCCTCAG AGAGCAGTGA AAGCAGCGAA GAGAGTGAAA GTGGAGAAGG AGCAGAGGAG 
GAAAGCCCTA CTGACCGCCC CCGTGATGCA GATTCTGACT CTGTGAACAC TATGGTTGTC 
CATGAAGAGG AGGAAGGTGA AGGAGGAGAA GAAGAACGTG CTGGAGGATA TGGAGACCAA 
ACCATGCTTG TGCAGAGGAC TCCAGAGAAG CGCAGTCATA ATGGATACAC CAACCTGCCT 
GATGTGGTTC AGCCTTCTCA CTCTCCCACA GACTCGGCCT CCCACTCCTC CCCCGGGAAA 
GACTCTACTT ATGATTATCA GTCCAGGGGA TTGGTCAAGG CCTCTGGAAA ATCCTCTTTT 
ACTACCTTTG TGGACCTTGG AATGTACCAG CCATCAGGGG GTAGTGGAGA TAACATTTCT 
GTTGGAGGTC TGGGCTCTCG TTTTGAGCAG CTAAAGCTGG AGGTGAGGAA GGGCTCCATG 
GTCAATGTCA ATCCCACAAA CACACGTCCG GTCAGTGACA CTCCTGAAAT CCGCAAATAC 
AAGAAGAGAT TTAACTCTGA GATCCTCTGC GCTGCTCTCT GGGGTGTGAA TCTGCTGGTC 
GGGACAGAGA ATGGTCTGAA GCTGCTGGAC AGAAGCGGTC AGGGTAAAGT GTATCCACTG 
ATCAACTCGC GCAGATTTCA GCAGATGGAT GTCCTGGAGG GACTGAACCT CCTCATCACT 
ATATCAGGCA AGAAGAATAA GGTACGCGTG TATTATCTGG CCTGGCTGAG GAATAAGATC 
CTCCATAATG ACCCAGAGGT GGAGAAGAAA CAGGGCTGGA CCACTGTAGG GGAAATGGAG 
GGCTGTGTGC ACTATAAAGT GGTGAAATAT GAAAGGATTA AGTTTCTGGT TATCGCTCTG 
AAGAATGCAG TGGAGGTTTA CGCCTGGGCT CCAAAACCTT ACCACAAGTT CATGGCCTTC 
AAGTCATTTG GAGACCTGCC GCACAGACCT GTGTTGGTTG ACCTCACAGT GGAAGAGGGT 
CAGAGGTTAA AGGTCATCTA TGGCTCCTTG GCTGGCTTCC ATGCCATTGA TGTTGACTCT 
GGAAACAACT ACGACATCTA CATTCCTGTA CATATCCAGT CACAGATCAC TCCTCACGCC 
ATCGTGTTCC TGCCCAACTC AGATGGGATG GAGATGCTGT TGTGTTATGA GGACGAAGGT 
GTTTATGTCA ACACGTACGG ACGCATCATT AAAGATGTGG TGCTGCAGTG GGGAGAAATG 
CCCACATCAG TAGCCCACAT TTGCTCTAAT CAGATTATGG GTTGGGGAGA GAAAGCCATT 
GAGATCCGTT CTGTTGAGAC GGGACACCTG GATGGAGTCT TCATGCACAA GAGAGCCCAG 
AGGCTTAAGT TCCTGTGTGA ACGGAACGAC AAGGTGTTTT TCGCGTCAGT GCGTTCCGGT 
GGCAGCAGCC AAGTTTACTT CATGACCCTC AACAGAAACT GCATTATGAA CTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mink1 ( XM_017356005.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017211494.1 Danio rerio misshapen-like kinase 1 (mink1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ODa59033
Clone ID Related Accession (Same CDS sequence)	XM_017356005.2 , XM_017356005.1
Accession Version	XM_017356005.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3909bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	misshapen-like kinase 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017356005.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGAAA ACGCACCTAC GCGGAGTCTG GATGACATCG ACCTGGCCGC CCTGCGGGAC 
CCAGCAGGGA TCTTCGAGTT GGTGGAGGTC GTGGGCAACG GCACCTATGG ACAAGTTTAC 
AAGGGTCGTC ATGTTAAGAC AGGTCAGCTG GCAGCCATAA AAGTCATGGA TGTGACTGAG 
GAAGAAGAGG AGGAGATTAA AGCGGAGATT AACATGCTGA AGAAGTACAG TCACCATAGA 
AATATCGCCA CCTACTATGG AGCCTTTGTG AAGAAGAGTC CTCCTGGACA CGATGACCAG 
CTCTGGTTGG TGATGGAGTT TTGTGGTGCC GGCTCAGTAA CAGACTTGGT GAAGAACACA 
AAAGGCAACT CTTTAAAAGA AGACTGGATC GCCTATATCT GCAGAGAGAT CCTTAGGGGT 
CTGTCTCACC TCCATGCTCA TAAAGTCATC CACCGAGACA TCAAGGGACA GAATGTGCTG 
CTTACAGAGA ACGCAGAGGT TAAACTGGTG GATTTTGGGG TGAGCGCACA GTTGGATCGT 
ACTGTCGGCA GAAGAAACAC TTTCATTGGC ACTCCATACT GGATGGCCCC AGAAGTCATT 
GCCTGTGATG AAAATCCCGA CTCCACCTAT GATTACAGGA GTGATATCTG GTCTCTGGGA 
ATCACTGCTA TAGAAATGGC TGAAGGAGCA CCTCCGTTGT GTGACATGCA TCCCATGCGA 
GCTTTGTTCC TTATCCCACG GAACCCTCCT CCCAAACTGA AGTCCAAGAA ATGGTCTAAG 
AAGTTCATTG ACTTCATCGA GGGCTGTCTG GTAAAGTCAT ATCCGAATCG TCCGTCCACT 
GAACAGCTCC TGAAACACTC ATTCATCAGA GATCAGCCCA CTGAAAGACA AGTCCGCATC 
CAGCTCAAAG ATCACATCGA CAGAACACGC AAGAAACGTG GCGAAAAAGA GGAGACAGAG 
TACGAGTACA GCGGCAGTGA TGAAGAGGAT GAGAACAGAG GAGATGAGAG AGAATCCAGC 
TCTATTTTGA ATGTTCCTGG TGAGTCGACG TTGCGGAGGG ATTTCCTGCG GCTGCAGCAA 
GAAAATAAAG AGCGTTCAGA GGCCTTAAAG AGACAGCAGG CGCAGCTCGC CGCTCAGCGC 
AGAGACCCTG AGGAGCATAA ACGCCAGCTG CTGCACGACC GGCAGAAGCG CATAGAGGAG 
CAGAAAGAGC AGAGGCGACG CTTAGAGGAG CAACAGAGGA AGGAGAGAGA GATGGTGCGG 
CAACAACAGG ATAAAGCTCC TCACCGGAGA CTCGATGACA TGAGACGAGA GGAGGACAGG 
AGGTTGGCAG AGAGAGAGCA GGAGTTTATA AGACATAAGT TGGAGGAAGA GCAGCGGCAG 
TTAGAGATAC TGCAGCAGCA ATTACTGCAG GAGCAGGCAT TGCTAATGGA GTATAAGCGT 
AAACAGCTGG AGGAGCAGAG ACAGTCAGAG AGGCTTCAGA GGCAGTTACA GCAGGAACAT 
GCGTATCTGG TCTCTCTACA GCAACAGCAG CAGCAGCAAG ACAAGAAACC ACAGATGTAT 
CACTACAGCA AGAACCTAGA GAACAACAAA CCAGCCTGGG CTAGAGAGGT CGAGGAGCGC 
AGTAAGCTGA ACAGACAGGG CTCTCCCAAA ATCTGCACCA CGGTCTCTGA CACTAATATT 
CAATCCCGCT CTGAGTCAAT CAACCAATCA GGAGCTGCGC AGACGCCGCC CATGCAGAGA 
CCTGTGGAGC CGCAGGGTGG ACAGGGAAAG TTCCAGATGG CCCATCTAGT GCCGTTAAAA 
CCATACGCCG CTCCAGTCCC TCGCTCTCAG TCTCTTTGTG ATCAGCCCAC TAAGACCATG 
TCCGCCTTCC CCACCCAGGA CCCCTCACCC ACACCCACAC CTCGTCCTCT ACACTCCAGA 
GAACTAGTCC GTCAAAACTC TGACCCCACC TCAGAGACTC CTGCCCCTCA GTCCCGGCCA 
ATGAGAGACG ACCGCGGACC CTGGATCCGT CTTCCTGAGA TTGAACAACC TCCTAAAATT 
CCTCAGAGAA CAGCCTCTAT AGCCACTGCA CTGAACACAA ATCTCTCCTC TGGTATCAGA 
CACCCAGTCC GAGCAAGTAA TCCAGATCTG AGTCGCAATG AGCGCTGGGA GAGAGGAGAC 
AGCATGAGCA TCATGTCCAA CCTGCCACAG ACCGGCTCTC TGGAGAGACA CCGCATACTC 
AGCTCATCTA AGATGGATTC CTCTCCAACC CTCTCTCATG ATGGCCGACA CAAACCAGGA 
GAATCCCGAA CCTCATCTAG ACCCAGCAGA CCAGCGAGCT ATAAGAGAGC AATAGGAGAG 
GATCATGGTC TGTACTCTAA GGAGCGAGCA GAGGAGCAGC CGCGGCCCCC TGTGAAGGCC 
AACGACTACT CCTCTTCCTC AGAGAGCAGT GAAAGCAGCG AAGAGAGTGA AAGTGGAGAA 
GGAGCAGAGG AGGAAAGCCC TACTGACCGC CCCCGTGATG CAGATTCTGA CTCTGTGAAC 
ACTATGGTTG TCCATGAAGA GGAGGAAGGT GAAGGAGGAG AAGAAGAACG TGCTGGAGGA 
TATGGAGACC AAACCATGCT TGTGCAGAGG ACTCCAGAGA AGCGCAGTCA TAATGGATAC 
ACCAACCTGC CTGATGTGGT TCAGCCTTCT CACTCTCCCA CAGACTCGGC CTCCCACTCC 
TCCCCCGGGA AAGACTCTAC TTATGATTAT CAGTCCAGGG GATTGGTCAA GGCCTCTGGA 
AAATCCTCTT TTACTACCTT TGTGGACCTT GGAATGTACC AGCCATCAGG GGGTAGTGGA 
GATAACATTT CTGTTGGAGG TCTGGGCTCT CGTTTTGAGC AGCTAAAGCT GGAGGTGAGG 
AAGGGCTCCA TGGTCAATGT CAATCCCACA AACACACGTC CGGTCAGTGA CACTCCTGAA 
ATCCGCAAAT ACAAGAAGAG ATTTAACTCT GAGATCCTCT GCGCTGCTCT CTGGGGTGTG 
AATCTGCTGG TCGGGACAGA GAATGGTCTG AAGCTGCTGG ACAGAAGCGG TCAGGGTAAA 
GTGTATCCAC TGATCAACTC GCGCAGATTT CAGCAGATGG ATGTCCTGGA GGGACTGAAC 
CTCCTCATCA CTATATCAGG CAAGAAGAAT AAGGTACGCG TGTATTATCT GGCCTGGCTG 
AGGAATAAGA TCCTCCATAA TGACCCAGAG GTGGAGAAGA AACAGGGCTG GACCACTGTA 
GGGGAAATGG AGGGCTGTGT GCACTATAAA GTGGTGAAAT ATGAAAGGAT TAAGTTTCTG 
GTTATCGCTC TGAAGAATGC AGTGGAGGTT TACGCCTGGG CTCCAAAACC TTACCACAAG 
TTCATGGCCT TCAAGTCATT TGGAGACCTG CCGCACAGAC CTGTGTTGGT TGACCTCACA 
GTGGAAGAGG GTCAGAGGTT AAAGGTCATC TATGGCTCCT TGGCTGGCTT CCATGCCATT 
GATGTTGACT CTGGAAACAA CTACGACATC TACATTCCTG TACATATCCA GTCACAGATC 
ACTCCTCACG CCATCGTGTT CCTGCCCAAC TCAGATGGGA TGGAGATGCT GTTGTGTTAT 
GAGGACGAAG GTGTTTATGT CAACACGTAC GGACGCATCA TTAAAGATGT GGTGCTGCAG 
TGGGGAGAAA TGCCCACATC AGTAGCCCAC ATTTGCTCTA ATCAGATTAT GGGTTGGGGA 
GAGAAAGCCA TTGAGATCCG TTCTGTTGAG ACGGGACACC TGGATGGAGT CTTCATGCAC 
AAGAGAGCCC AGAGGCTTAA GTTCCTGTGT GAACGGAACG ACAAGGTGTT TTTCGCGTCA 
GTGCGTTCCG GTGGCAGCAG CCAAGTTTAC TTCATGACCC TCAACAGAAA CTGCATTATG 
AACTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017211494.1
CDS	1261..5169
Translation	MSENAPTRSLDDIDLAALRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEEEEEEIKAEINMLKKYSHHRNIATYYGAFVKKSPPGHDDQLWLVMEFCGAGSVTDLVKNTKGNSLKEDWIAYICREILRGLSHLHAHKVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDSTYDYRSDIWSLGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPKLKSKKWSKKFIDFIEGCLVKSYPNRPSTEQLLKHSFIRDQPTERQVRIQLKDHIDRTRKKRGEKEETEYEYSGSDEEDENRGDERESSSILNVPGESTLRRDFLRLQQENKERSEALKRQQAQLAAQRRDPEEHKRQLLHDRQKRIEEQKEQRRRLEEQQRKEREMVRQQQDKAPHRRLDDMRREEDRRLAEREQEFIRHKLEEEQRQLEILQQQLLQEQALLMEYKRKQLEEQRQSERLQRQLQQEHAYLVSLQQQQQQQDKKPQMYHYSKNLENNKPAWAREVEERSKLNRQGSPKICTTVSDTNIQSRSESINQSGAAQTPPMQRPVEPQGGQGKFQMAHLVPLKPYAAPVPRSQSLCDQPTKTMSAFPTQDPSPTPTPRPLHSRELVRQNSDPTSETPAPQSRPMRDDRGPWIRLPEIEQPPKIPQRTASIATALNTNLSSGIRHPVRASNPDLSRNERWERGDSMSIMSNLPQTGSLERHRILSSSKMDSSPTLSHDGRHKPGESRTSSRPSRPASYKRAIGEDHGLYSKERAEEQPRPPVKANDYSSSSESSESSEESESGEGAEEESPTDRPRDADSDSVNTMVVHEEEEGEGGEEERAGGYGDQTMLVQRTPEKRSHNGYTNLPDVVQPSHSPTDSASHSSPGKDSTYDYQSRGLVKASGKSSFTTFVDLGMYQPSGGSGDNISVGGLGSRFEQLKLEVRKGSMVNVNPTNTRPVSDTPEIRKYKKRFNSEILCAALWGVNLLVGTENGLKLLDRSGQGKVYPLINSRRFQQMDVLEGLNLLITISGKKNKVRVYYLAWLRNKILHNDPEVEKKQGWTTVGEMEGCVHYKVVKYERIKFLVIALKNAVEVYAWAPKPYHKFMAFKSFGDLPHRPVLVDLTVEEGQRLKVIYGSLAGFHAIDVDSGNNYDIYIPVHIQSQITPHAIVFLPNSDGMEMLLCYEDEGVYVNTYGRIIKDVVLQWGEMPTSVAHICSNQIMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLNRNCIMNW

Target ORF information:

RefSeq Version	XM_017356005.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio misshapen-like kinase 1 (mink1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017356005.2

ORF Insert Sequence:

ATGTCAGAAA ACGCACCTAC GCGGAGTCTG GATGACATCG ACCTGGCCGC CCTGCGGGAC 
CCAGCAGGGA TCTTCGAGTT GGTGGAGGTC GTGGGCAACG GCACCTATGG ACAAGTTTAC 
AAGGGTCGTC ATGTTAAGAC AGGTCAGCTG GCAGCCATAA AAGTCATGGA TGTGACTGAG 
GAAGAAGAGG AGGAGATTAA AGCGGAGATT AACATGCTGA AGAAGTACAG TCACCATAGA 
AATATCGCCA CCTACTATGG AGCCTTTGTG AAGAAGAGTC CTCCTGGACA CGATGACCAG 
CTCTGGTTGG TGATGGAGTT TTGTGGTGCC GGCTCAGTAA CAGACTTGGT GAAGAACACA 
AAAGGCAACT CTTTAAAAGA AGACTGGATC GCCTATATCT GCAGAGAGAT CCTTAGGGGT 
CTGTCTCACC TCCATGCTCA TAAAGTCATC CACCGAGACA TCAAGGGACA GAATGTGCTG 
CTTACAGAGA ACGCAGAGGT TAAACTGGTG GATTTTGGGG TGAGCGCACA GTTGGATCGT 
ACTGTCGGCA GAAGAAACAC TTTCATTGGC ACTCCATACT GGATGGCCCC AGAAGTCATT 
GCCTGTGATG AAAATCCCGA CTCCACCTAT GATTACAGGA GTGATATCTG GTCTCTGGGA 
ATCACTGCTA TAGAAATGGC TGAAGGAGCA CCTCCGTTGT GTGACATGCA TCCCATGCGA 
GCTTTGTTCC TTATCCCACG GAACCCTCCT CCCAAACTGA AGTCCAAGAA ATGGTCTAAG 
AAGTTCATTG ACTTCATCGA GGGCTGTCTG GTAAAGTCAT ATCCGAATCG TCCGTCCACT 
GAACAGCTCC TGAAACACTC ATTCATCAGA GATCAGCCCA CTGAAAGACA AGTCCGCATC 
CAGCTCAAAG ATCACATCGA CAGAACACGC AAGAAACGTG GCGAAAAAGA GGAGACAGAG 
TACGAGTACA GCGGCAGTGA TGAAGAGGAT GAGAACAGAG GAGATGAGAG AGAATCCAGC 
TCTATTTTGA ATGTTCCTGG TGAGTCGACG TTGCGGAGGG ATTTCCTGCG GCTGCAGCAA 
GAAAATAAAG AGCGTTCAGA GGCCTTAAAG AGACAGCAGG CGCAGCTCGC CGCTCAGCGC 
AGAGACCCTG AGGAGCATAA ACGCCAGCTG CTGCACGACC GGCAGAAGCG CATAGAGGAG 
CAGAAAGAGC AGAGGCGACG CTTAGAGGAG CAACAGAGGA AGGAGAGAGA GATGGTGCGG 
CAACAACAGG ATAAAGCTCC TCACCGGAGA CTCGATGACA TGAGACGAGA GGAGGACAGG 
AGGTTGGCAG AGAGAGAGCA GGAGTTTATA AGACATAAGT TGGAGGAAGA GCAGCGGCAG 
TTAGAGATAC TGCAGCAGCA ATTACTGCAG GAGCAGGCAT TGCTAATGGA GTATAAGCGT 
AAACAGCTGG AGGAGCAGAG ACAGTCAGAG AGGCTTCAGA GGCAGTTACA GCAGGAACAT 
GCGTATCTGG TCTCTCTACA GCAACAGCAG CAGCAGCAAG ACAAGAAACC ACAGATGTAT 
CACTACAGCA AGAACCTAGA GAACAACAAA CCAGCCTGGG CTAGAGAGGT CGAGGAGCGC 
AGTAAGCTGA ACAGACAGGG CTCTCCCAAA ATCTGCACCA CGGTCTCTGA CACTAATATT 
CAATCCCGCT CTGAGTCAAT CAACCAATCA GGAGCTGCGC AGACGCCGCC CATGCAGAGA 
CCTGTGGAGC CGCAGGGTGG ACAGGGAAAG TTCCAGATGG CCCATCTAGT GCCGTTAAAA 
CCATACGCCG CTCCAGTCCC TCGCTCTCAG TCTCTTTGTG ATCAGCCCAC TAAGACCATG 
TCCGCCTTCC CCACCCAGGA CCCCTCACCC ACACCCACAC CTCGTCCTCT ACACTCCAGA 
GAACTAGTCC GTCAAAACTC TGACCCCACC TCAGAGACTC CTGCCCCTCA GTCCCGGCCA 
ATGAGAGACG ACCGCGGACC CTGGATCCGT CTTCCTGAGA TTGAACAACC TCCTAAAATT 
CCTCAGAGAA CAGCCTCTAT AGCCACTGCA CTGAACACAA ATCTCTCCTC TGGTATCAGA 
CACCCAGTCC GAGCAAGTAA TCCAGATCTG AGTCGCAATG AGCGCTGGGA GAGAGGAGAC 
AGCATGAGCA TCATGTCCAA CCTGCCACAG ACCGGCTCTC TGGAGAGACA CCGCATACTC 
AGCTCATCTA AGATGGATTC CTCTCCAACC CTCTCTCATG ATGGCCGACA CAAACCAGGA 
GAATCCCGAA CCTCATCTAG ACCCAGCAGA CCAGCGAGCT ATAAGAGAGC AATAGGAGAG 
GATCATGGTC TGTACTCTAA GGAGCGAGCA GAGGAGCAGC CGCGGCCCCC TGTGAAGGCC 
AACGACTACT CCTCTTCCTC AGAGAGCAGT GAAAGCAGCG AAGAGAGTGA AAGTGGAGAA 
GGAGCAGAGG AGGAAAGCCC TACTGACCGC CCCCGTGATG CAGATTCTGA CTCTGTGAAC 
ACTATGGTTG TCCATGAAGA GGAGGAAGGT GAAGGAGGAG AAGAAGAACG TGCTGGAGGA 
TATGGAGACC AAACCATGCT TGTGCAGAGG ACTCCAGAGA AGCGCAGTCA TAATGGATAC 
ACCAACCTGC CTGATGTGGT TCAGCCTTCT CACTCTCCCA CAGACTCGGC CTCCCACTCC 
TCCCCCGGGA AAGACTCTAC TTATGATTAT CAGTCCAGGG GATTGGTCAA GGCCTCTGGA 
AAATCCTCTT TTACTACCTT TGTGGACCTT GGAATGTACC AGCCATCAGG GGGTAGTGGA 
GATAACATTT CTGTTGGAGG TCTGGGCTCT CGTTTTGAGC AGCTAAAGCT GGAGGTGAGG 
AAGGGCTCCA TGGTCAATGT CAATCCCACA AACACACGTC CGGTCAGTGA CACTCCTGAA 
ATCCGCAAAT ACAAGAAGAG ATTTAACTCT GAGATCCTCT GCGCTGCTCT CTGGGGTGTG 
AATCTGCTGG TCGGGACAGA GAATGGTCTG AAGCTGCTGG ACAGAAGCGG TCAGGGTAAA 
GTGTATCCAC TGATCAACTC GCGCAGATTT CAGCAGATGG ATGTCCTGGA GGGACTGAAC 
CTCCTCATCA CTATATCAGG CAAGAAGAAT AAGGTACGCG TGTATTATCT GGCCTGGCTG 
AGGAATAAGA TCCTCCATAA TGACCCAGAG GTGGAGAAGA AACAGGGCTG GACCACTGTA 
GGGGAAATGG AGGGCTGTGT GCACTATAAA GTGGTGAAAT ATGAAAGGAT TAAGTTTCTG 
GTTATCGCTC TGAAGAATGC AGTGGAGGTT TACGCCTGGG CTCCAAAACC TTACCACAAG 
TTCATGGCCT TCAAGTCATT TGGAGACCTG CCGCACAGAC CTGTGTTGGT TGACCTCACA 
GTGGAAGAGG GTCAGAGGTT AAAGGTCATC TATGGCTCCT TGGCTGGCTT CCATGCCATT 
GATGTTGACT CTGGAAACAA CTACGACATC TACATTCCTG TACATATCCA GTCACAGATC 
ACTCCTCACG CCATCGTGTT CCTGCCCAAC TCAGATGGGA TGGAGATGCT GTTGTGTTAT 
GAGGACGAAG GTGTTTATGT CAACACGTAC GGACGCATCA TTAAAGATGT GGTGCTGCAG 
TGGGGAGAAA TGCCCACATC AGTAGCCCAC ATTTGCTCTA ATCAGATTAT GGGTTGGGGA 
GAGAAAGCCA TTGAGATCCG TTCTGTTGAG ACGGGACACC TGGATGGAGT CTTCATGCAC 
AAGAGAGCCC AGAGGCTTAA GTTCCTGTGT GAACGGAACG ACAAGGTGTT TTTCGCGTCA 
GTGCGTTCCG GTGGCAGCAG CCAAGTTTAC TTCATGACCC TCAACAGAAA CTGCATTATG 
AACTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mink1 ( XM_017356005.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017211494.1 Danio rerio misshapen-like kinase 1 (mink1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa59033
Clone ID Related Accession (Same CDS sequence)	XM_017356005.2 , XM_017356005.1
Accession Version	XM_017356005.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3909bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	misshapen-like kinase 1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGAAA ACGCACCTAC GCGGAGTCTG GATGACATCG ACCTGGCCGC CCTGCGGGAC 
CCAGCAGGGA TCTTCGAGTT GGTGGAGGTC GTGGGCAACG GCACCTATGG ACAAGTTTAC 
AAGGGTCGTC ATGTTAAGAC AGGTCAGCTG GCAGCCATAA AAGTCATGGA TGTGACTGAG 
GAAGAAGAGG AGGAGATTAA AGCGGAGATT AACATGCTGA AGAAGTACAG TCACCATAGA 
AATATCGCCA CCTACTATGG AGCCTTTGTG AAGAAGAGTC CTCCTGGACA CGATGACCAG 
CTCTGGTTGG TGATGGAGTT TTGTGGTGCC GGCTCAGTAA CAGACTTGGT GAAGAACACA 
AAAGGCAACT CTTTAAAAGA AGACTGGATC GCCTATATCT GCAGAGAGAT CCTTAGGGGT 
CTGTCTCACC TCCATGCTCA TAAAGTCATC CACCGAGACA TCAAGGGACA GAATGTGCTG 
CTTACAGAGA ACGCAGAGGT TAAACTGGTG GATTTTGGGG TGAGCGCACA GTTGGATCGT 
ACTGTCGGCA GAAGAAACAC TTTCATTGGC ACTCCATACT GGATGGCCCC AGAAGTCATT 
GCCTGTGATG AAAATCCCGA CTCCACCTAT GATTACAGGA GTGATATCTG GTCTCTGGGA 
ATCACTGCTA TAGAAATGGC TGAAGGAGCA CCTCCGTTGT GTGACATGCA TCCCATGCGA 
GCTTTGTTCC TTATCCCACG GAACCCTCCT CCCAAACTGA AGTCCAAGAA ATGGTCTAAG 
AAGTTCATTG ACTTCATCGA GGGCTGTCTG GTAAAGTCAT ATCCGAATCG TCCGTCCACT 
GAACAGCTCC TGAAACACTC ATTCATCAGA GATCAGCCCA CTGAAAGACA AGTCCGCATC 
CAGCTCAAAG ATCACATCGA CAGAACACGC AAGAAACGTG GCGAAAAAGA GGAGACAGAG 
TACGAGTACA GCGGCAGTGA TGAAGAGGAT GAGAACAGAG GAGATGAGAG AGAATCCAGC 
TCTATTTTGA ATGTTCCTGG TGAGTCGACG TTGCGGAGGG ATTTCCTGCG GCTGCAGCAA 
GAAAATAAAG AGCGTTCAGA GGCCTTAAAG AGACAGCAGG CGCAGCTCGC CGCTCAGCGC 
AGAGACCCTG AGGAGCATAA ACGCCAGCTG CTGCACGACC GGCAGAAGCG CATAGAGGAG 
CAGAAAGAGC AGAGGCGACG CTTAGAGGAG CAACAGAGGA AGGAGAGAGA GATGGTGCGG 
CAACAACAGG ATAAAGCTCC TCACCGGAGA CTCGATGACA TGAGACGAGA GGAGGACAGG 
AGGTTGGCAG AGAGAGAGCA GGAGTTTATA AGACATAAGT TGGAGGAAGA GCAGCGGCAG 
TTAGAGATAC TGCAGCAGCA ATTACTGCAG GAGCAGGCAT TGCTAATGGA GTATAAGCGT 
AAACAGCTGG AGGAGCAGAG ACAGTCAGAG AGGCTTCAGA GGCAGTTACA GCAGGAACAT 
GCGTATCTGG TCTCTCTACA GCAACAGCAG CAGCAGCAAG ACAAGAAACC ACAGATGTAT 
CACTACAGCA AGAACCTAGA GAACAACAAA CCAGCCTGGG CTAGAGAGGT CGAGGAGCGC 
AGTAAGCTGA ACAGACAGGG CTCTCCCAAA ATCTGCACCA CGGTCTCTGA CACTAATATT 
CAATCCCGCT CTGAGTCAAT CAACCAATCA GGAGCTGCGC AGACGCCGCC CATGCAGAGA 
CCTGTGGAGC CGCAGGGTGG ACAGGGAAAG TTCCAGATGG CCCATCTAGT GCCGTTAAAA 
CCATACGCCG CTCCAGTCCC TCGCTCTCAG TCTCTTTGTG ATCAGCCCAC TAAGACCATG 
TCCGCCTTCC CCACCCAGGA CCCCTCACCC ACACCCACAC CTCGTCCTCT ACACTCCAGA 
GAACTAGTCC GTCAAAACTC TGACCCCACC TCAGAGACTC CTGCCCCTCA GTCCCGGCCA 
ATGAGAGACG ACCGCGGACC CTGGATCCGT CTTCCTGAGA TTGAACAACC TCCTAAAATT 
CCTCAGAGAA CAGCCTCTAT AGCCACTGCA CTGAACACAA ATCTCTCCTC TGGTATCAGA 
CACCCAGTCC GAGCAAGTAA TCCAGATCTG AGTCGCAATG AGCGCTGGGA GAGAGGAGAC 
AGCATGAGCA TCATGTCCAA CCTGCCACAG ACCGGCTCTC TGGAGAGACA CCGCATACTC 
AGCTCATCTA AGATGGATTC CTCTCCAACC CTCTCTCATG ATGGCCGACA CAAACCAGGA 
GAATCCCGAA CCTCATCTAG ACCCAGCAGA CCAGCGAGCT ATAAGAGAGC AATAGGAGAG 
GATCATGGTC TGTACTCTAA GGAGCGAGCA GAGGAGCAGC CGCGGCCCCC TGTGAAGGCC 
AACGACTACT CCTCTTCCTC AGAGAGCAGT GAAAGCAGCG AAGAGAGTGA AAGTGGAGAA 
GGAGCAGAGG AGGAAAGCCC TACTGACCGC CCCCGTGATG CAGATTCTGA CTCTGTGAAC 
ACTATGGTTG TCCATGAAGA GGAGGAAGGT GAAGGAGGAG AAGAAGAACG TGCTGGAGGA 
TATGGAGACC AAACCATGCT TGTGCAGAGG ACTCCAGAGA AGCGCAGTCA TAATGGATAC 
ACCAACCTGC CTGATGTGGT TCAGCCTTCT CACTCTCCCA CAGACTCGGC CTCCCACTCC 
TCCCCCGGGA AAGACTCTAC TTATGATTAT CAGTCCAGGG GATTGGTCAA GGCCTCTGGA 
AAATCCTCTT TTACTACCTT TGTGGACCTT GGAATGTACC AGCCATCAGG GGGTAGTGGA 
GATAACATTT CTGTTGGAGG TCTGGGCTCT CGTTTTGAGC AGCTAAAGCT GGAGGTGAGG 
AAGGGCTCCA TGGTCAATGT CAATCCCACA AACACACGTC CGGTCAGTGA CACTCCTGAA 
ATCCGCAAAT ACAAGAAGAG ATTTAACTCT GAGATCCTCT GCGCTGCTCT CTGGGGTGTG 
AATCTGCTGG TCGGGACAGA GAATGGTCTG AAGCTGCTGG ACAGAAGCGG TCAGGGTAAA 
GTGTATCCAC TGATCAACTC GCGCAGATTT CAGCAGATGG ATGTCCTGGA GGGACTGAAC 
CTCCTCATCA CTATATCAGG CAAGAAGAAT AAGGTACGCG TGTATTATCT GGCCTGGCTG 
AGGAATAAGA TCCTCCATAA TGACCCAGAG GTGGAGAAGA AACAGGGCTG GACCACTGTA 
GGGGAAATGG AGGGCTGTGT GCACTATAAA GTGGTGAAAT ATGAAAGGAT TAAGTTTCTG 
GTTATCGCTC TGAAGAATGC AGTGGAGGTT TACGCCTGGG CTCCAAAACC TTACCACAAG 
TTCATGGCCT TCAAGTCATT TGGAGACCTG CCGCACAGAC CTGTGTTGGT TGACCTCACA 
GTGGAAGAGG GTCAGAGGTT AAAGGTCATC TATGGCTCCT TGGCTGGCTT CCATGCCATT 
GATGTTGACT CTGGAAACAA CTACGACATC TACATTCCTG TACATATCCA GTCACAGATC 
ACTCCTCACG CCATCGTGTT CCTGCCCAAC TCAGATGGGA TGGAGATGCT GTTGTGTTAT 
GAGGACGAAG GTGTTTATGT CAACACGTAC GGACGCATCA TTAAAGATGT GGTGCTGCAG 
TGGGGAGAAA TGCCCACATC AGTAGCCCAC ATTTGCTCTA ATCAGATTAT GGGTTGGGGA 
GAGAAAGCCA TTGAGATCCG TTCTGTTGAG ACGGGACACC TGGATGGAGT CTTCATGCAC 
AAGAGAGCCC AGAGGCTTAA GTTCCTGTGT GAACGGAACG ACAAGGTGTT TTTCGCGTCA 
GTGCGTTCCG GTGGCAGCAG CCAAGTTTAC TTCATGACCC TCAACAGAAA CTGCATTATG 
AACTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017211494.1
CDS	1244..5152
Translation	MSENAPTRSLDDIDLAALRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEEEEEEIKAEINMLKKYSHHRNIATYYGAFVKKSPPGHDDQLWLVMEFCGAGSVTDLVKNTKGNSLKEDWIAYICREILRGLSHLHAHKVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDSTYDYRSDIWSLGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPKLKSKKWSKKFIDFIEGCLVKSYPNRPSTEQLLKHSFIRDQPTERQVRIQLKDHIDRTRKKRGEKEETEYEYSGSDEEDENRGDERESSSILNVPGESTLRRDFLRLQQENKERSEALKRQQAQLAAQRRDPEEHKRQLLHDRQKRIEEQKEQRRRLEEQQRKEREMVRQQQDKAPHRRLDDMRREEDRRLAEREQEFIRHKLEEEQRQLEILQQQLLQEQALLMEYKRKQLEEQRQSERLQRQLQQEHAYLVSLQQQQQQQDKKPQMYHYSKNLENNKPAWAREVEERSKLNRQGSPKICTTVSDTNIQSRSESINQSGAAQTPPMQRPVEPQGGQGKFQMAHLVPLKPYAAPVPRSQSLCDQPTKTMSAFPTQDPSPTPTPRPLHSRELVRQNSDPTSETPAPQSRPMRDDRGPWIRLPEIEQPPKIPQRTASIATALNTNLSSGIRHPVRASNPDLSRNERWERGDSMSIMSNLPQTGSLERHRILSSSKMDSSPTLSHDGRHKPGESRTSSRPSRPASYKRAIGEDHGLYSKERAEEQPRPPVKANDYSSSSESSESSEESESGEGAEEESPTDRPRDADSDSVNTMVVHEEEEGEGGEEERAGGYGDQTMLVQRTPEKRSHNGYTNLPDVVQPSHSPTDSASHSSPGKDSTYDYQSRGLVKASGKSSFTTFVDLGMYQPSGGSGDNISVGGLGSRFEQLKLEVRKGSMVNVNPTNTRPVSDTPEIRKYKKRFNSEILCAALWGVNLLVGTENGLKLLDRSGQGKVYPLINSRRFQQMDVLEGLNLLITISGKKNKVRVYYLAWLRNKILHNDPEVEKKQGWTTVGEMEGCVHYKVVKYERIKFLVIALKNAVEVYAWAPKPYHKFMAFKSFGDLPHRPVLVDLTVEEGQRLKVIYGSLAGFHAIDVDSGNNYDIYIPVHIQSQITPHAIVFLPNSDGMEMLLCYEDEGVYVNTYGRIIKDVVLQWGEMPTSVAHICSNQIMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLNRNCIMNW

Target ORF information:

RefSeq Version	XM_017356005.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio misshapen-like kinase 1 (mink1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017356005.1

ORF Insert Sequence:

ATGTCAGAAA ACGCACCTAC GCGGAGTCTG GATGACATCG ACCTGGCCGC CCTGCGGGAC 
CCAGCAGGGA TCTTCGAGTT GGTGGAGGTC GTGGGCAACG GCACCTATGG ACAAGTTTAC 
AAGGGTCGTC ATGTTAAGAC AGGTCAGCTG GCAGCCATAA AAGTCATGGA TGTGACTGAG 
GAAGAAGAGG AGGAGATTAA AGCGGAGATT AACATGCTGA AGAAGTACAG TCACCATAGA 
AATATCGCCA CCTACTATGG AGCCTTTGTG AAGAAGAGTC CTCCTGGACA CGATGACCAG 
CTCTGGTTGG TGATGGAGTT TTGTGGTGCC GGCTCAGTAA CAGACTTGGT GAAGAACACA 
AAAGGCAACT CTTTAAAAGA AGACTGGATC GCCTATATCT GCAGAGAGAT CCTTAGGGGT 
CTGTCTCACC TCCATGCTCA TAAAGTCATC CACCGAGACA TCAAGGGACA GAATGTGCTG 
CTTACAGAGA ACGCAGAGGT TAAACTGGTG GATTTTGGGG TGAGCGCACA GTTGGATCGT 
ACTGTCGGCA GAAGAAACAC TTTCATTGGC ACTCCATACT GGATGGCCCC AGAAGTCATT 
GCCTGTGATG AAAATCCCGA CTCCACCTAT GATTACAGGA GTGATATCTG GTCTCTGGGA 
ATCACTGCTA TAGAAATGGC TGAAGGAGCA CCTCCGTTGT GTGACATGCA TCCCATGCGA 
GCTTTGTTCC TTATCCCACG GAACCCTCCT CCCAAACTGA AGTCCAAGAA ATGGTCTAAG 
AAGTTCATTG ACTTCATCGA GGGCTGTCTG GTAAAGTCAT ATCCGAATCG TCCGTCCACT 
GAACAGCTCC TGAAACACTC ATTCATCAGA GATCAGCCCA CTGAAAGACA AGTCCGCATC 
CAGCTCAAAG ATCACATCGA CAGAACACGC AAGAAACGTG GCGAAAAAGA GGAGACAGAG 
TACGAGTACA GCGGCAGTGA TGAAGAGGAT GAGAACAGAG GAGATGAGAG AGAATCCAGC 
TCTATTTTGA ATGTTCCTGG TGAGTCGACG TTGCGGAGGG ATTTCCTGCG GCTGCAGCAA 
GAAAATAAAG AGCGTTCAGA GGCCTTAAAG AGACAGCAGG CGCAGCTCGC CGCTCAGCGC 
AGAGACCCTG AGGAGCATAA ACGCCAGCTG CTGCACGACC GGCAGAAGCG CATAGAGGAG 
CAGAAAGAGC AGAGGCGACG CTTAGAGGAG CAACAGAGGA AGGAGAGAGA GATGGTGCGG 
CAACAACAGG ATAAAGCTCC TCACCGGAGA CTCGATGACA TGAGACGAGA GGAGGACAGG 
AGGTTGGCAG AGAGAGAGCA GGAGTTTATA AGACATAAGT TGGAGGAAGA GCAGCGGCAG 
TTAGAGATAC TGCAGCAGCA ATTACTGCAG GAGCAGGCAT TGCTAATGGA GTATAAGCGT 
AAACAGCTGG AGGAGCAGAG ACAGTCAGAG AGGCTTCAGA GGCAGTTACA GCAGGAACAT 
GCGTATCTGG TCTCTCTACA GCAACAGCAG CAGCAGCAAG ACAAGAAACC ACAGATGTAT 
CACTACAGCA AGAACCTAGA GAACAACAAA CCAGCCTGGG CTAGAGAGGT CGAGGAGCGC 
AGTAAGCTGA ACAGACAGGG CTCTCCCAAA ATCTGCACCA CGGTCTCTGA CACTAATATT 
CAATCCCGCT CTGAGTCAAT CAACCAATCA GGAGCTGCGC AGACGCCGCC CATGCAGAGA 
CCTGTGGAGC CGCAGGGTGG ACAGGGAAAG TTCCAGATGG CCCATCTAGT GCCGTTAAAA 
CCATACGCCG CTCCAGTCCC TCGCTCTCAG TCTCTTTGTG ATCAGCCCAC TAAGACCATG 
TCCGCCTTCC CCACCCAGGA CCCCTCACCC ACACCCACAC CTCGTCCTCT ACACTCCAGA 
GAACTAGTCC GTCAAAACTC TGACCCCACC TCAGAGACTC CTGCCCCTCA GTCCCGGCCA 
ATGAGAGACG ACCGCGGACC CTGGATCCGT CTTCCTGAGA TTGAACAACC TCCTAAAATT 
CCTCAGAGAA CAGCCTCTAT AGCCACTGCA CTGAACACAA ATCTCTCCTC TGGTATCAGA 
CACCCAGTCC GAGCAAGTAA TCCAGATCTG AGTCGCAATG AGCGCTGGGA GAGAGGAGAC 
AGCATGAGCA TCATGTCCAA CCTGCCACAG ACCGGCTCTC TGGAGAGACA CCGCATACTC 
AGCTCATCTA AGATGGATTC CTCTCCAACC CTCTCTCATG ATGGCCGACA CAAACCAGGA 
GAATCCCGAA CCTCATCTAG ACCCAGCAGA CCAGCGAGCT ATAAGAGAGC AATAGGAGAG 
GATCATGGTC TGTACTCTAA GGAGCGAGCA GAGGAGCAGC CGCGGCCCCC TGTGAAGGCC 
AACGACTACT CCTCTTCCTC AGAGAGCAGT GAAAGCAGCG AAGAGAGTGA AAGTGGAGAA 
GGAGCAGAGG AGGAAAGCCC TACTGACCGC CCCCGTGATG CAGATTCTGA CTCTGTGAAC 
ACTATGGTTG TCCATGAAGA GGAGGAAGGT GAAGGAGGAG AAGAAGAACG TGCTGGAGGA 
TATGGAGACC AAACCATGCT TGTGCAGAGG ACTCCAGAGA AGCGCAGTCA TAATGGATAC 
ACCAACCTGC CTGATGTGGT TCAGCCTTCT CACTCTCCCA CAGACTCGGC CTCCCACTCC 
TCCCCCGGGA AAGACTCTAC TTATGATTAT CAGTCCAGGG GATTGGTCAA GGCCTCTGGA 
AAATCCTCTT TTACTACCTT TGTGGACCTT GGAATGTACC AGCCATCAGG GGGTAGTGGA 
GATAACATTT CTGTTGGAGG TCTGGGCTCT CGTTTTGAGC AGCTAAAGCT GGAGGTGAGG 
AAGGGCTCCA TGGTCAATGT CAATCCCACA AACACACGTC CGGTCAGTGA CACTCCTGAA 
ATCCGCAAAT ACAAGAAGAG ATTTAACTCT GAGATCCTCT GCGCTGCTCT CTGGGGTGTG 
AATCTGCTGG TCGGGACAGA GAATGGTCTG AAGCTGCTGG ACAGAAGCGG TCAGGGTAAA 
GTGTATCCAC TGATCAACTC GCGCAGATTT CAGCAGATGG ATGTCCTGGA GGGACTGAAC 
CTCCTCATCA CTATATCAGG CAAGAAGAAT AAGGTACGCG TGTATTATCT GGCCTGGCTG 
AGGAATAAGA TCCTCCATAA TGACCCAGAG GTGGAGAAGA AACAGGGCTG GACCACTGTA 
GGGGAAATGG AGGGCTGTGT GCACTATAAA GTGGTGAAAT ATGAAAGGAT TAAGTTTCTG 
GTTATCGCTC TGAAGAATGC AGTGGAGGTT TACGCCTGGG CTCCAAAACC TTACCACAAG 
TTCATGGCCT TCAAGTCATT TGGAGACCTG CCGCACAGAC CTGTGTTGGT TGACCTCACA 
GTGGAAGAGG GTCAGAGGTT AAAGGTCATC TATGGCTCCT TGGCTGGCTT CCATGCCATT 
GATGTTGACT CTGGAAACAA CTACGACATC TACATTCCTG TACATATCCA GTCACAGATC 
ACTCCTCACG CCATCGTGTT CCTGCCCAAC TCAGATGGGA TGGAGATGCT GTTGTGTTAT 
GAGGACGAAG GTGTTTATGT CAACACGTAC GGACGCATCA TTAAAGATGT GGTGCTGCAG 
TGGGGAGAAA TGCCCACATC AGTAGCCCAC ATTTGCTCTA ATCAGATTAT GGGTTGGGGA 
GAGAAAGCCA TTGAGATCCG TTCTGTTGAG ACGGGACACC TGGATGGAGT CTTCATGCAC 
AAGAGAGCCC AGAGGCTTAA GTTCCTGTGT GAACGGAACG ACAAGGTGTT TTTCGCGTCA 
GTGCGTTCCG GTGGCAGCAG CCAAGTTTAC TTCATGACCC TCAACAGAAA CTGCATTATG 
AACTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mink1 ( XM_017356006.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017211495.1 Danio rerio misshapen-like kinase 1 (mink1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa59034
Clone ID Related Accession (Same CDS sequence)	XM_017356006.1 , XM_017356006.2
Accession Version	XM_017356006.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3903bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	misshapen-like kinase 1 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGAAA ACGCACCTAC GCGGAGTCTG GATGACATCG ACCTGGCCGC CCTGCGGGAC 
CCAGCAGGGA TCTTCGAGTT GGTGGAGGTC GTGGGCAACG GCACCTATGG ACAAGTTTAC 
AAGGGTCGTC ATGTTAAGAC AGGTCAGCTG GCAGCCATAA AAGTCATGGA TGTGACTGAG 
GAAGAAGAGG AGGAGATTAA AGCGGAGATT AACATGCTGA AGAAGTACAG TCACCATAGA 
AATATCGCCA CCTACTATGG AGCCTTTGTG AAGAAGAGTC CTCCTGGACA CGATGACCAG 
CTCTGGTTGG TGATGGAGTT TTGTGGTGCC GGCTCAGTAA CAGACTTGGT GAAGAACACA 
AAAGGCAACT CTTTAAAAGA AGACTGGATC GCCTATATCT GCAGAGAGAT CCTTAGGGGT 
CTGTCTCACC TCCATGCTCA TAAAGTCATC CACCGAGACA TCAAGGGACA GAATGTGCTG 
CTTACAGAGA ACGCAGAGGT TAAACTGGTG GATTTTGGGG TGAGCGCACA GTTGGATCGT 
ACTGTCGGCA GAAGAAACAC TTTCATTGGC ACTCCATACT GGATGGCCCC AGAAGTCATT 
GCCTGTGATG AAAATCCCGA CTCCACCTAT GATTACAGGA GTGATATCTG GTCTCTGGGA 
ATCACTGCTA TAGAAATGGC TGAAGGAGCA CCTCCGTTGT GTGACATGCA TCCCATGCGA 
GCTTTGTTCC TTATCCCACG GAACCCTCCT CCCAAACTGA AGTCCAAGAA ATGGTCTAAG 
AAGTTCATTG ACTTCATCGA GGGCTGTCTG GTAAAGTCAT ATCCGAATCG TCCGTCCACT 
GAACAGCTCC TGAAACACTC ATTCATCAGA GATCAGCCCA CTGAAAGACA AGTCCGCATC 
CAGCTCAAAG ATCACATCGA CAGAACACGC AAGAAACGTG GCGAAAAAGA GGAGACAGAG 
TACGAGTACA GCGGCAGTGA TGAAGAGGAT GAGAACAGAG GAGATGAGAG AGAATCCAGC 
TCTATTTTGA ATGTTCCTGG TGAGTCGACG TTGCGGAGGG ATTTCCTGCG GCTGCAGCAA 
GAAAATAAAG AGCGTTCAGA GGCCTTAAAG AGACAGCAGG CGCAGCTCGC CGCTCAGCGC 
AGAGACCCTG AGGAGCATAA ACGCCAGCTG CTGCACGACC GGCAGAAGCG CATAGAGGAG 
CAGAAAGAGC AGAGGCGACG CTTAGAGGAG CAACAGAGGA AGGAGAGAGA GATGGTGCGG 
CAACAACAGG ATAAAGCTCC TCACCGGAGA CTCGATGACA TGAGACGAGA GGAGGACAGG 
AGGTTGGCAG AGAGAGAGCA GGAGTTTATA AGACATAAGT TGGAGGAAGA GCAGCGGCAG 
TTAGAGATAC TGCAGCAGCA ATTACTGCAG GAGCAGGCAT TGCTAATGGA GTATAAGCGT 
AAACAGCTGG AGGAGCAGAG ACAGTCAGAG AGGCTTCAGA GGCAGTTACA GCAGGAACAT 
GCGTATCTGG TCTCTCTACA GCAACAGCAG CAGCAGCAAG ACAAGAAACC ACAGATGTAT 
CACTACAGCA AGAACCTAGA GAACAACAAA CCAGCCTGGG CTAGAGAGGT CGAGGAGCGC 
AGTAAGCTGA ACAGACAGGG CTCTCCCAAA ATCTGCACCA CGGTCTCTGA CACTAATATT 
CAATCCCGCT CTGAGTCAAT CAACCAATCA GGAGCTGCGC AGACGCCGCC CATGCAGAGA 
CCTGTGGAGC CGCAGGGTGG ACAGGGAAAG ATGGCCCATC TAGTGCCGTT AAAACCATAC 
GCCGCTCCAG TCCCTCGCTC TCAGTCTCTT TGTGATCAGC CCACTAAGAC CATGTCCGCC 
TTCCCCACCC AGGACCCCTC ACCCACACCC ACACCTCGTC CTCTACACTC CAGAGAACTA 
GTCCGTCAAA ACTCTGACCC CACCTCAGAG ACTCCTGCCC CTCAGTCCCG GCCAATGAGA 
GACGACCGCG GACCCTGGAT CCGTCTTCCT GAGATTGAAC AACCTCCTAA AATTCCTCAG 
AGAACAGCCT CTATAGCCAC TGCACTGAAC ACAAATCTCT CCTCTGGTAT CAGACACCCA 
GTCCGAGCAA GTAATCCAGA TCTGAGTCGC AATGAGCGCT GGGAGAGAGG AGACAGCATG 
AGCATCATGT CCAACCTGCC ACAGACCGGC TCTCTGGAGA GACACCGCAT ACTCAGCTCA 
TCTAAGATGG ATTCCTCTCC AACCCTCTCT CATGATGGCC GACACAAACC AGGAGAATCC 
CGAACCTCAT CTAGACCCAG CAGACCAGCG AGCTATAAGA GAGCAATAGG AGAGGATCAT 
GGTCTGTACT CTAAGGAGCG AGCAGAGGAG CAGCCGCGGC CCCCTGTGAA GGCCAACGAC 
TACTCCTCTT CCTCAGAGAG CAGTGAAAGC AGCGAAGAGA GTGAAAGTGG AGAAGGAGCA 
GAGGAGGAAA GCCCTACTGA CCGCCCCCGT GATGCAGATT CTGACTCTGT GAACACTATG 
GTTGTCCATG AAGAGGAGGA AGGTGAAGGA GGAGAAGAAG AACGTGCTGG AGGATATGGA 
GACCAAACCA TGCTTGTGCA GAGGACTCCA GAGAAGCGCA GTCATAATGG ATACACCAAC 
CTGCCTGATG TGGTTCAGCC TTCTCACTCT CCCACAGACT CGGCCTCCCA CTCCTCCCCC 
GGGAAAGACT CTACTTATGA TTATCAGTCC AGGGGATTGG TCAAGGCCTC TGGAAAATCC 
TCTTTTACTA CCTTTGTGGA CCTTGGAATG TACCAGCCAT CAGGGGGTAG TGGAGATAAC 
ATTTCTGTTG GAGGTCTGGG CTCTCGTTTT GAGCAGCTAA AGCTGGAGGT GAGGAAGGGC 
TCCATGGTCA ATGTCAATCC CACAAACACA CGTCCGGTCA GTGACACTCC TGAAATCCGC 
AAATACAAGA AGAGATTTAA CTCTGAGATC CTCTGCGCTG CTCTCTGGGG TGTGAATCTG 
CTGGTCGGGA CAGAGAATGG TCTGAAGCTG CTGGACAGAA GCGGTCAGGG TAAAGTGTAT 
CCACTGATCA ACTCGCGCAG ATTTCAGCAG ATGGATGTCC TGGAGGGACT GAACCTCCTC 
ATCACTATAT CAGGCAAGAA GAATAAGGTA CGCGTGTATT ATCTGGCCTG GCTGAGGAAT 
AAGATCCTCC ATAATGACCC AGAGGTGGAG AAGAAACAGG GCTGGACCAC TGTAGGGGAA 
ATGGAGGGCT GTGTGCACTA TAAAGTGGTG AAATATGAAA GGATTAAGTT TCTGGTTATC 
GCTCTGAAGA ATGCAGTGGA GGTTTACGCC TGGGCTCCAA AACCTTACCA CAAGTTCATG 
GCCTTCAAGT CATTTGGAGA CCTGCCGCAC AGACCTGTGT TGGTTGACCT CACAGTGGAA 
GAGGGTCAGA GGTTAAAGGT CATCTATGGC TCCTTGGCTG GCTTCCATGC CATTGATGTT 
GACTCTGGAA ACAACTACGA CATCTACATT CCTGTACATA TCCAGTCACA GATCACTCCT 
CACGCCATCG TGTTCCTGCC CAACTCAGAT GGGATGGAGA TGCTGTTGTG TTATGAGGAC 
GAAGGTGTTT ATGTCAACAC GTACGGACGC ATCATTAAAG ATGTGGTGCT GCAGTGGGGA 
GAAATGCCCA CATCAGTAGC CCACATTTGC TCTAATCAGA TTATGGGTTG GGGAGAGAAA 
GCCATTGAGA TCCGTTCTGT TGAGACGGGA CACCTGGATG GAGTCTTCAT GCACAAGAGA 
GCCCAGAGGC TTAAGTTCCT GTGTGAACGG AACGACAAGG TGTTTTTCGC GTCAGTGCGT 
TCCGGTGGCA GCAGCCAAGT TTACTTCATG ACCCTCAACA GAAACTGCAT TATGAACTGG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017211495.1
CDS	1245..5147
Translation	MSENAPTRSLDDIDLAALRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEEEEEEIKAEINMLKKYSHHRNIATYYGAFVKKSPPGHDDQLWLVMEFCGAGSVTDLVKNTKGNSLKEDWIAYICREILRGLSHLHAHKVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDSTYDYRSDIWSLGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPKLKSKKWSKKFIDFIEGCLVKSYPNRPSTEQLLKHSFIRDQPTERQVRIQLKDHIDRTRKKRGEKEETEYEYSGSDEEDENRGDERESSSILNVPGESTLRRDFLRLQQENKERSEALKRQQAQLAAQRRDPEEHKRQLLHDRQKRIEEQKEQRRRLEEQQRKEREMVRQQQDKAPHRRLDDMRREEDRRLAEREQEFIRHKLEEEQRQLEILQQQLLQEQALLMEYKRKQLEEQRQSERLQRQLQQEHAYLVSLQQQQQQQDKKPQMYHYSKNLENNKPAWAREVEERSKLNRQGSPKICTTVSDTNIQSRSESINQSGAAQTPPMQRPVEPQGGQGKMAHLVPLKPYAAPVPRSQSLCDQPTKTMSAFPTQDPSPTPTPRPLHSRELVRQNSDPTSETPAPQSRPMRDDRGPWIRLPEIEQPPKIPQRTASIATALNTNLSSGIRHPVRASNPDLSRNERWERGDSMSIMSNLPQTGSLERHRILSSSKMDSSPTLSHDGRHKPGESRTSSRPSRPASYKRAIGEDHGLYSKERAEEQPRPPVKANDYSSSSESSESSEESESGEGAEEESPTDRPRDADSDSVNTMVVHEEEEGEGGEEERAGGYGDQTMLVQRTPEKRSHNGYTNLPDVVQPSHSPTDSASHSSPGKDSTYDYQSRGLVKASGKSSFTTFVDLGMYQPSGGSGDNISVGGLGSRFEQLKLEVRKGSMVNVNPTNTRPVSDTPEIRKYKKRFNSEILCAALWGVNLLVGTENGLKLLDRSGQGKVYPLINSRRFQQMDVLEGLNLLITISGKKNKVRVYYLAWLRNKILHNDPEVEKKQGWTTVGEMEGCVHYKVVKYERIKFLVIALKNAVEVYAWAPKPYHKFMAFKSFGDLPHRPVLVDLTVEEGQRLKVIYGSLAGFHAIDVDSGNNYDIYIPVHIQSQITPHAIVFLPNSDGMEMLLCYEDEGVYVNTYGRIIKDVVLQWGEMPTSVAHICSNQIMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLNRNCIMNW

Target ORF information:

RefSeq Version	XM_017356006.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio misshapen-like kinase 1 (mink1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017356006.1

ORF Insert Sequence:

ATGTCAGAAA ACGCACCTAC GCGGAGTCTG GATGACATCG ACCTGGCCGC CCTGCGGGAC 
CCAGCAGGGA TCTTCGAGTT GGTGGAGGTC GTGGGCAACG GCACCTATGG ACAAGTTTAC 
AAGGGTCGTC ATGTTAAGAC AGGTCAGCTG GCAGCCATAA AAGTCATGGA TGTGACTGAG 
GAAGAAGAGG AGGAGATTAA AGCGGAGATT AACATGCTGA AGAAGTACAG TCACCATAGA 
AATATCGCCA CCTACTATGG AGCCTTTGTG AAGAAGAGTC CTCCTGGACA CGATGACCAG 
CTCTGGTTGG TGATGGAGTT TTGTGGTGCC GGCTCAGTAA CAGACTTGGT GAAGAACACA 
AAAGGCAACT CTTTAAAAGA AGACTGGATC GCCTATATCT GCAGAGAGAT CCTTAGGGGT 
CTGTCTCACC TCCATGCTCA TAAAGTCATC CACCGAGACA TCAAGGGACA GAATGTGCTG 
CTTACAGAGA ACGCAGAGGT TAAACTGGTG GATTTTGGGG TGAGCGCACA GTTGGATCGT 
ACTGTCGGCA GAAGAAACAC TTTCATTGGC ACTCCATACT GGATGGCCCC AGAAGTCATT 
GCCTGTGATG AAAATCCCGA CTCCACCTAT GATTACAGGA GTGATATCTG GTCTCTGGGA 
ATCACTGCTA TAGAAATGGC TGAAGGAGCA CCTCCGTTGT GTGACATGCA TCCCATGCGA 
GCTTTGTTCC TTATCCCACG GAACCCTCCT CCCAAACTGA AGTCCAAGAA ATGGTCTAAG 
AAGTTCATTG ACTTCATCGA GGGCTGTCTG GTAAAGTCAT ATCCGAATCG TCCGTCCACT 
GAACAGCTCC TGAAACACTC ATTCATCAGA GATCAGCCCA CTGAAAGACA AGTCCGCATC 
CAGCTCAAAG ATCACATCGA CAGAACACGC AAGAAACGTG GCGAAAAAGA GGAGACAGAG 
TACGAGTACA GCGGCAGTGA TGAAGAGGAT GAGAACAGAG GAGATGAGAG AGAATCCAGC 
TCTATTTTGA ATGTTCCTGG TGAGTCGACG TTGCGGAGGG ATTTCCTGCG GCTGCAGCAA 
GAAAATAAAG AGCGTTCAGA GGCCTTAAAG AGACAGCAGG CGCAGCTCGC CGCTCAGCGC 
AGAGACCCTG AGGAGCATAA ACGCCAGCTG CTGCACGACC GGCAGAAGCG CATAGAGGAG 
CAGAAAGAGC AGAGGCGACG CTTAGAGGAG CAACAGAGGA AGGAGAGAGA GATGGTGCGG 
CAACAACAGG ATAAAGCTCC TCACCGGAGA CTCGATGACA TGAGACGAGA GGAGGACAGG 
AGGTTGGCAG AGAGAGAGCA GGAGTTTATA AGACATAAGT TGGAGGAAGA GCAGCGGCAG 
TTAGAGATAC TGCAGCAGCA ATTACTGCAG GAGCAGGCAT TGCTAATGGA GTATAAGCGT 
AAACAGCTGG AGGAGCAGAG ACAGTCAGAG AGGCTTCAGA GGCAGTTACA GCAGGAACAT 
GCGTATCTGG TCTCTCTACA GCAACAGCAG CAGCAGCAAG ACAAGAAACC ACAGATGTAT 
CACTACAGCA AGAACCTAGA GAACAACAAA CCAGCCTGGG CTAGAGAGGT CGAGGAGCGC 
AGTAAGCTGA ACAGACAGGG CTCTCCCAAA ATCTGCACCA CGGTCTCTGA CACTAATATT 
CAATCCCGCT CTGAGTCAAT CAACCAATCA GGAGCTGCGC AGACGCCGCC CATGCAGAGA 
CCTGTGGAGC CGCAGGGTGG ACAGGGAAAG ATGGCCCATC TAGTGCCGTT AAAACCATAC 
GCCGCTCCAG TCCCTCGCTC TCAGTCTCTT TGTGATCAGC CCACTAAGAC CATGTCCGCC 
TTCCCCACCC AGGACCCCTC ACCCACACCC ACACCTCGTC CTCTACACTC CAGAGAACTA 
GTCCGTCAAA ACTCTGACCC CACCTCAGAG ACTCCTGCCC CTCAGTCCCG GCCAATGAGA 
GACGACCGCG GACCCTGGAT CCGTCTTCCT GAGATTGAAC AACCTCCTAA AATTCCTCAG 
AGAACAGCCT CTATAGCCAC TGCACTGAAC ACAAATCTCT CCTCTGGTAT CAGACACCCA 
GTCCGAGCAA GTAATCCAGA TCTGAGTCGC AATGAGCGCT GGGAGAGAGG AGACAGCATG 
AGCATCATGT CCAACCTGCC ACAGACCGGC TCTCTGGAGA GACACCGCAT ACTCAGCTCA 
TCTAAGATGG ATTCCTCTCC AACCCTCTCT CATGATGGCC GACACAAACC AGGAGAATCC 
CGAACCTCAT CTAGACCCAG CAGACCAGCG AGCTATAAGA GAGCAATAGG AGAGGATCAT 
GGTCTGTACT CTAAGGAGCG AGCAGAGGAG CAGCCGCGGC CCCCTGTGAA GGCCAACGAC 
TACTCCTCTT CCTCAGAGAG CAGTGAAAGC AGCGAAGAGA GTGAAAGTGG AGAAGGAGCA 
GAGGAGGAAA GCCCTACTGA CCGCCCCCGT GATGCAGATT CTGACTCTGT GAACACTATG 
GTTGTCCATG AAGAGGAGGA AGGTGAAGGA GGAGAAGAAG AACGTGCTGG AGGATATGGA 
GACCAAACCA TGCTTGTGCA GAGGACTCCA GAGAAGCGCA GTCATAATGG ATACACCAAC 
CTGCCTGATG TGGTTCAGCC TTCTCACTCT CCCACAGACT CGGCCTCCCA CTCCTCCCCC 
GGGAAAGACT CTACTTATGA TTATCAGTCC AGGGGATTGG TCAAGGCCTC TGGAAAATCC 
TCTTTTACTA CCTTTGTGGA CCTTGGAATG TACCAGCCAT CAGGGGGTAG TGGAGATAAC 
ATTTCTGTTG GAGGTCTGGG CTCTCGTTTT GAGCAGCTAA AGCTGGAGGT GAGGAAGGGC 
TCCATGGTCA ATGTCAATCC CACAAACACA CGTCCGGTCA GTGACACTCC TGAAATCCGC 
AAATACAAGA AGAGATTTAA CTCTGAGATC CTCTGCGCTG CTCTCTGGGG TGTGAATCTG 
CTGGTCGGGA CAGAGAATGG TCTGAAGCTG CTGGACAGAA GCGGTCAGGG TAAAGTGTAT 
CCACTGATCA ACTCGCGCAG ATTTCAGCAG ATGGATGTCC TGGAGGGACT GAACCTCCTC 
ATCACTATAT CAGGCAAGAA GAATAAGGTA CGCGTGTATT ATCTGGCCTG GCTGAGGAAT 
AAGATCCTCC ATAATGACCC AGAGGTGGAG AAGAAACAGG GCTGGACCAC TGTAGGGGAA 
ATGGAGGGCT GTGTGCACTA TAAAGTGGTG AAATATGAAA GGATTAAGTT TCTGGTTATC 
GCTCTGAAGA ATGCAGTGGA GGTTTACGCC TGGGCTCCAA AACCTTACCA CAAGTTCATG 
GCCTTCAAGT CATTTGGAGA CCTGCCGCAC AGACCTGTGT TGGTTGACCT CACAGTGGAA 
GAGGGTCAGA GGTTAAAGGT CATCTATGGC TCCTTGGCTG GCTTCCATGC CATTGATGTT 
GACTCTGGAA ACAACTACGA CATCTACATT CCTGTACATA TCCAGTCACA GATCACTCCT 
CACGCCATCG TGTTCCTGCC CAACTCAGAT GGGATGGAGA TGCTGTTGTG TTATGAGGAC 
GAAGGTGTTT ATGTCAACAC GTACGGACGC ATCATTAAAG ATGTGGTGCT GCAGTGGGGA 
GAAATGCCCA CATCAGTAGC CCACATTTGC TCTAATCAGA TTATGGGTTG GGGAGAGAAA 
GCCATTGAGA TCCGTTCTGT TGAGACGGGA CACCTGGATG GAGTCTTCAT GCACAAGAGA 
GCCCAGAGGC TTAAGTTCCT GTGTGAACGG AACGACAAGG TGTTTTTCGC GTCAGTGCGT 
TCCGGTGGCA GCAGCCAAGT TTACTTCATG ACCCTCAACA GAAACTGCAT TATGAACTGG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mink1 ( XM_017356006.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017211495.1 Danio rerio misshapen-like kinase 1 (mink1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	ODa59034
Clone ID Related Accession (Same CDS sequence)	XM_017356006.1 , XM_017356006.2
Accession Version	XM_017356006.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3903bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	misshapen-like kinase 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017356006.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGAAA ACGCACCTAC GCGGAGTCTG GATGACATCG ACCTGGCCGC CCTGCGGGAC 
CCAGCAGGGA TCTTCGAGTT GGTGGAGGTC GTGGGCAACG GCACCTATGG ACAAGTTTAC 
AAGGGTCGTC ATGTTAAGAC AGGTCAGCTG GCAGCCATAA AAGTCATGGA TGTGACTGAG 
GAAGAAGAGG AGGAGATTAA AGCGGAGATT AACATGCTGA AGAAGTACAG TCACCATAGA 
AATATCGCCA CCTACTATGG AGCCTTTGTG AAGAAGAGTC CTCCTGGACA CGATGACCAG 
CTCTGGTTGG TGATGGAGTT TTGTGGTGCC GGCTCAGTAA CAGACTTGGT GAAGAACACA 
AAAGGCAACT CTTTAAAAGA AGACTGGATC GCCTATATCT GCAGAGAGAT CCTTAGGGGT 
CTGTCTCACC TCCATGCTCA TAAAGTCATC CACCGAGACA TCAAGGGACA GAATGTGCTG 
CTTACAGAGA ACGCAGAGGT TAAACTGGTG GATTTTGGGG TGAGCGCACA GTTGGATCGT 
ACTGTCGGCA GAAGAAACAC TTTCATTGGC ACTCCATACT GGATGGCCCC AGAAGTCATT 
GCCTGTGATG AAAATCCCGA CTCCACCTAT GATTACAGGA GTGATATCTG GTCTCTGGGA 
ATCACTGCTA TAGAAATGGC TGAAGGAGCA CCTCCGTTGT GTGACATGCA TCCCATGCGA 
GCTTTGTTCC TTATCCCACG GAACCCTCCT CCCAAACTGA AGTCCAAGAA ATGGTCTAAG 
AAGTTCATTG ACTTCATCGA GGGCTGTCTG GTAAAGTCAT ATCCGAATCG TCCGTCCACT 
GAACAGCTCC TGAAACACTC ATTCATCAGA GATCAGCCCA CTGAAAGACA AGTCCGCATC 
CAGCTCAAAG ATCACATCGA CAGAACACGC AAGAAACGTG GCGAAAAAGA GGAGACAGAG 
TACGAGTACA GCGGCAGTGA TGAAGAGGAT GAGAACAGAG GAGATGAGAG AGAATCCAGC 
TCTATTTTGA ATGTTCCTGG TGAGTCGACG TTGCGGAGGG ATTTCCTGCG GCTGCAGCAA 
GAAAATAAAG AGCGTTCAGA GGCCTTAAAG AGACAGCAGG CGCAGCTCGC CGCTCAGCGC 
AGAGACCCTG AGGAGCATAA ACGCCAGCTG CTGCACGACC GGCAGAAGCG CATAGAGGAG 
CAGAAAGAGC AGAGGCGACG CTTAGAGGAG CAACAGAGGA AGGAGAGAGA GATGGTGCGG 
CAACAACAGG ATAAAGCTCC TCACCGGAGA CTCGATGACA TGAGACGAGA GGAGGACAGG 
AGGTTGGCAG AGAGAGAGCA GGAGTTTATA AGACATAAGT TGGAGGAAGA GCAGCGGCAG 
TTAGAGATAC TGCAGCAGCA ATTACTGCAG GAGCAGGCAT TGCTAATGGA GTATAAGCGT 
AAACAGCTGG AGGAGCAGAG ACAGTCAGAG AGGCTTCAGA GGCAGTTACA GCAGGAACAT 
GCGTATCTGG TCTCTCTACA GCAACAGCAG CAGCAGCAAG ACAAGAAACC ACAGATGTAT 
CACTACAGCA AGAACCTAGA GAACAACAAA CCAGCCTGGG CTAGAGAGGT CGAGGAGCGC 
AGTAAGCTGA ACAGACAGGG CTCTCCCAAA ATCTGCACCA CGGTCTCTGA CACTAATATT 
CAATCCCGCT CTGAGTCAAT CAACCAATCA GGAGCTGCGC AGACGCCGCC CATGCAGAGA 
CCTGTGGAGC CGCAGGGTGG ACAGGGAAAG ATGGCCCATC TAGTGCCGTT AAAACCATAC 
GCCGCTCCAG TCCCTCGCTC TCAGTCTCTT TGTGATCAGC CCACTAAGAC CATGTCCGCC 
TTCCCCACCC AGGACCCCTC ACCCACACCC ACACCTCGTC CTCTACACTC CAGAGAACTA 
GTCCGTCAAA ACTCTGACCC CACCTCAGAG ACTCCTGCCC CTCAGTCCCG GCCAATGAGA 
GACGACCGCG GACCCTGGAT CCGTCTTCCT GAGATTGAAC AACCTCCTAA AATTCCTCAG 
AGAACAGCCT CTATAGCCAC TGCACTGAAC ACAAATCTCT CCTCTGGTAT CAGACACCCA 
GTCCGAGCAA GTAATCCAGA TCTGAGTCGC AATGAGCGCT GGGAGAGAGG AGACAGCATG 
AGCATCATGT CCAACCTGCC ACAGACCGGC TCTCTGGAGA GACACCGCAT ACTCAGCTCA 
TCTAAGATGG ATTCCTCTCC AACCCTCTCT CATGATGGCC GACACAAACC AGGAGAATCC 
CGAACCTCAT CTAGACCCAG CAGACCAGCG AGCTATAAGA GAGCAATAGG AGAGGATCAT 
GGTCTGTACT CTAAGGAGCG AGCAGAGGAG CAGCCGCGGC CCCCTGTGAA GGCCAACGAC 
TACTCCTCTT CCTCAGAGAG CAGTGAAAGC AGCGAAGAGA GTGAAAGTGG AGAAGGAGCA 
GAGGAGGAAA GCCCTACTGA CCGCCCCCGT GATGCAGATT CTGACTCTGT GAACACTATG 
GTTGTCCATG AAGAGGAGGA AGGTGAAGGA GGAGAAGAAG AACGTGCTGG AGGATATGGA 
GACCAAACCA TGCTTGTGCA GAGGACTCCA GAGAAGCGCA GTCATAATGG ATACACCAAC 
CTGCCTGATG TGGTTCAGCC TTCTCACTCT CCCACAGACT CGGCCTCCCA CTCCTCCCCC 
GGGAAAGACT CTACTTATGA TTATCAGTCC AGGGGATTGG TCAAGGCCTC TGGAAAATCC 
TCTTTTACTA CCTTTGTGGA CCTTGGAATG TACCAGCCAT CAGGGGGTAG TGGAGATAAC 
ATTTCTGTTG GAGGTCTGGG CTCTCGTTTT GAGCAGCTAA AGCTGGAGGT GAGGAAGGGC 
TCCATGGTCA ATGTCAATCC CACAAACACA CGTCCGGTCA GTGACACTCC TGAAATCCGC 
AAATACAAGA AGAGATTTAA CTCTGAGATC CTCTGCGCTG CTCTCTGGGG TGTGAATCTG 
CTGGTCGGGA CAGAGAATGG TCTGAAGCTG CTGGACAGAA GCGGTCAGGG TAAAGTGTAT 
CCACTGATCA ACTCGCGCAG ATTTCAGCAG ATGGATGTCC TGGAGGGACT GAACCTCCTC 
ATCACTATAT CAGGCAAGAA GAATAAGGTA CGCGTGTATT ATCTGGCCTG GCTGAGGAAT 
AAGATCCTCC ATAATGACCC AGAGGTGGAG AAGAAACAGG GCTGGACCAC TGTAGGGGAA 
ATGGAGGGCT GTGTGCACTA TAAAGTGGTG AAATATGAAA GGATTAAGTT TCTGGTTATC 
GCTCTGAAGA ATGCAGTGGA GGTTTACGCC TGGGCTCCAA AACCTTACCA CAAGTTCATG 
GCCTTCAAGT CATTTGGAGA CCTGCCGCAC AGACCTGTGT TGGTTGACCT CACAGTGGAA 
GAGGGTCAGA GGTTAAAGGT CATCTATGGC TCCTTGGCTG GCTTCCATGC CATTGATGTT 
GACTCTGGAA ACAACTACGA CATCTACATT CCTGTACATA TCCAGTCACA GATCACTCCT 
CACGCCATCG TGTTCCTGCC CAACTCAGAT GGGATGGAGA TGCTGTTGTG TTATGAGGAC 
GAAGGTGTTT ATGTCAACAC GTACGGACGC ATCATTAAAG ATGTGGTGCT GCAGTGGGGA 
GAAATGCCCA CATCAGTAGC CCACATTTGC TCTAATCAGA TTATGGGTTG GGGAGAGAAA 
GCCATTGAGA TCCGTTCTGT TGAGACGGGA CACCTGGATG GAGTCTTCAT GCACAAGAGA 
GCCCAGAGGC TTAAGTTCCT GTGTGAACGG AACGACAAGG TGTTTTTCGC GTCAGTGCGT 
TCCGGTGGCA GCAGCCAAGT TTACTTCATG ACCCTCAACA GAAACTGCAT TATGAACTGG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017211495.1
CDS	1261..5163
Translation	MSENAPTRSLDDIDLAALRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEEEEEEIKAEINMLKKYSHHRNIATYYGAFVKKSPPGHDDQLWLVMEFCGAGSVTDLVKNTKGNSLKEDWIAYICREILRGLSHLHAHKVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDSTYDYRSDIWSLGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPKLKSKKWSKKFIDFIEGCLVKSYPNRPSTEQLLKHSFIRDQPTERQVRIQLKDHIDRTRKKRGEKEETEYEYSGSDEEDENRGDERESSSILNVPGESTLRRDFLRLQQENKERSEALKRQQAQLAAQRRDPEEHKRQLLHDRQKRIEEQKEQRRRLEEQQRKEREMVRQQQDKAPHRRLDDMRREEDRRLAEREQEFIRHKLEEEQRQLEILQQQLLQEQALLMEYKRKQLEEQRQSERLQRQLQQEHAYLVSLQQQQQQQDKKPQMYHYSKNLENNKPAWAREVEERSKLNRQGSPKICTTVSDTNIQSRSESINQSGAAQTPPMQRPVEPQGGQGKMAHLVPLKPYAAPVPRSQSLCDQPTKTMSAFPTQDPSPTPTPRPLHSRELVRQNSDPTSETPAPQSRPMRDDRGPWIRLPEIEQPPKIPQRTASIATALNTNLSSGIRHPVRASNPDLSRNERWERGDSMSIMSNLPQTGSLERHRILSSSKMDSSPTLSHDGRHKPGESRTSSRPSRPASYKRAIGEDHGLYSKERAEEQPRPPVKANDYSSSSESSESSEESESGEGAEEESPTDRPRDADSDSVNTMVVHEEEEGEGGEEERAGGYGDQTMLVQRTPEKRSHNGYTNLPDVVQPSHSPTDSASHSSPGKDSTYDYQSRGLVKASGKSSFTTFVDLGMYQPSGGSGDNISVGGLGSRFEQLKLEVRKGSMVNVNPTNTRPVSDTPEIRKYKKRFNSEILCAALWGVNLLVGTENGLKLLDRSGQGKVYPLINSRRFQQMDVLEGLNLLITISGKKNKVRVYYLAWLRNKILHNDPEVEKKQGWTTVGEMEGCVHYKVVKYERIKFLVIALKNAVEVYAWAPKPYHKFMAFKSFGDLPHRPVLVDLTVEEGQRLKVIYGSLAGFHAIDVDSGNNYDIYIPVHIQSQITPHAIVFLPNSDGMEMLLCYEDEGVYVNTYGRIIKDVVLQWGEMPTSVAHICSNQIMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLNRNCIMNW

Target ORF information:

RefSeq Version	XM_017356006.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio misshapen-like kinase 1 (mink1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017356006.2

ORF Insert Sequence:

ATGTCAGAAA ACGCACCTAC GCGGAGTCTG GATGACATCG ACCTGGCCGC CCTGCGGGAC 
CCAGCAGGGA TCTTCGAGTT GGTGGAGGTC GTGGGCAACG GCACCTATGG ACAAGTTTAC 
AAGGGTCGTC ATGTTAAGAC AGGTCAGCTG GCAGCCATAA AAGTCATGGA TGTGACTGAG 
GAAGAAGAGG AGGAGATTAA AGCGGAGATT AACATGCTGA AGAAGTACAG TCACCATAGA 
AATATCGCCA CCTACTATGG AGCCTTTGTG AAGAAGAGTC CTCCTGGACA CGATGACCAG 
CTCTGGTTGG TGATGGAGTT TTGTGGTGCC GGCTCAGTAA CAGACTTGGT GAAGAACACA 
AAAGGCAACT CTTTAAAAGA AGACTGGATC GCCTATATCT GCAGAGAGAT CCTTAGGGGT 
CTGTCTCACC TCCATGCTCA TAAAGTCATC CACCGAGACA TCAAGGGACA GAATGTGCTG 
CTTACAGAGA ACGCAGAGGT TAAACTGGTG GATTTTGGGG TGAGCGCACA GTTGGATCGT 
ACTGTCGGCA GAAGAAACAC TTTCATTGGC ACTCCATACT GGATGGCCCC AGAAGTCATT 
GCCTGTGATG AAAATCCCGA CTCCACCTAT GATTACAGGA GTGATATCTG GTCTCTGGGA 
ATCACTGCTA TAGAAATGGC TGAAGGAGCA CCTCCGTTGT GTGACATGCA TCCCATGCGA 
GCTTTGTTCC TTATCCCACG GAACCCTCCT CCCAAACTGA AGTCCAAGAA ATGGTCTAAG 
AAGTTCATTG ACTTCATCGA GGGCTGTCTG GTAAAGTCAT ATCCGAATCG TCCGTCCACT 
GAACAGCTCC TGAAACACTC ATTCATCAGA GATCAGCCCA CTGAAAGACA AGTCCGCATC 
CAGCTCAAAG ATCACATCGA CAGAACACGC AAGAAACGTG GCGAAAAAGA GGAGACAGAG 
TACGAGTACA GCGGCAGTGA TGAAGAGGAT GAGAACAGAG GAGATGAGAG AGAATCCAGC 
TCTATTTTGA ATGTTCCTGG TGAGTCGACG TTGCGGAGGG ATTTCCTGCG GCTGCAGCAA 
GAAAATAAAG AGCGTTCAGA GGCCTTAAAG AGACAGCAGG CGCAGCTCGC CGCTCAGCGC 
AGAGACCCTG AGGAGCATAA ACGCCAGCTG CTGCACGACC GGCAGAAGCG CATAGAGGAG 
CAGAAAGAGC AGAGGCGACG CTTAGAGGAG CAACAGAGGA AGGAGAGAGA GATGGTGCGG 
CAACAACAGG ATAAAGCTCC TCACCGGAGA CTCGATGACA TGAGACGAGA GGAGGACAGG 
AGGTTGGCAG AGAGAGAGCA GGAGTTTATA AGACATAAGT TGGAGGAAGA GCAGCGGCAG 
TTAGAGATAC TGCAGCAGCA ATTACTGCAG GAGCAGGCAT TGCTAATGGA GTATAAGCGT 
AAACAGCTGG AGGAGCAGAG ACAGTCAGAG AGGCTTCAGA GGCAGTTACA GCAGGAACAT 
GCGTATCTGG TCTCTCTACA GCAACAGCAG CAGCAGCAAG ACAAGAAACC ACAGATGTAT 
CACTACAGCA AGAACCTAGA GAACAACAAA CCAGCCTGGG CTAGAGAGGT CGAGGAGCGC 
AGTAAGCTGA ACAGACAGGG CTCTCCCAAA ATCTGCACCA CGGTCTCTGA CACTAATATT 
CAATCCCGCT CTGAGTCAAT CAACCAATCA GGAGCTGCGC AGACGCCGCC CATGCAGAGA 
CCTGTGGAGC CGCAGGGTGG ACAGGGAAAG ATGGCCCATC TAGTGCCGTT AAAACCATAC 
GCCGCTCCAG TCCCTCGCTC TCAGTCTCTT TGTGATCAGC CCACTAAGAC CATGTCCGCC 
TTCCCCACCC AGGACCCCTC ACCCACACCC ACACCTCGTC CTCTACACTC CAGAGAACTA 
GTCCGTCAAA ACTCTGACCC CACCTCAGAG ACTCCTGCCC CTCAGTCCCG GCCAATGAGA 
GACGACCGCG GACCCTGGAT CCGTCTTCCT GAGATTGAAC AACCTCCTAA AATTCCTCAG 
AGAACAGCCT CTATAGCCAC TGCACTGAAC ACAAATCTCT CCTCTGGTAT CAGACACCCA 
GTCCGAGCAA GTAATCCAGA TCTGAGTCGC AATGAGCGCT GGGAGAGAGG AGACAGCATG 
AGCATCATGT CCAACCTGCC ACAGACCGGC TCTCTGGAGA GACACCGCAT ACTCAGCTCA 
TCTAAGATGG ATTCCTCTCC AACCCTCTCT CATGATGGCC GACACAAACC AGGAGAATCC 
CGAACCTCAT CTAGACCCAG CAGACCAGCG AGCTATAAGA GAGCAATAGG AGAGGATCAT 
GGTCTGTACT CTAAGGAGCG AGCAGAGGAG CAGCCGCGGC CCCCTGTGAA GGCCAACGAC 
TACTCCTCTT CCTCAGAGAG CAGTGAAAGC AGCGAAGAGA GTGAAAGTGG AGAAGGAGCA 
GAGGAGGAAA GCCCTACTGA CCGCCCCCGT GATGCAGATT CTGACTCTGT GAACACTATG 
GTTGTCCATG AAGAGGAGGA AGGTGAAGGA GGAGAAGAAG AACGTGCTGG AGGATATGGA 
GACCAAACCA TGCTTGTGCA GAGGACTCCA GAGAAGCGCA GTCATAATGG ATACACCAAC 
CTGCCTGATG TGGTTCAGCC TTCTCACTCT CCCACAGACT CGGCCTCCCA CTCCTCCCCC 
GGGAAAGACT CTACTTATGA TTATCAGTCC AGGGGATTGG TCAAGGCCTC TGGAAAATCC 
TCTTTTACTA CCTTTGTGGA CCTTGGAATG TACCAGCCAT CAGGGGGTAG TGGAGATAAC 
ATTTCTGTTG GAGGTCTGGG CTCTCGTTTT GAGCAGCTAA AGCTGGAGGT GAGGAAGGGC 
TCCATGGTCA ATGTCAATCC CACAAACACA CGTCCGGTCA GTGACACTCC TGAAATCCGC 
AAATACAAGA AGAGATTTAA CTCTGAGATC CTCTGCGCTG CTCTCTGGGG TGTGAATCTG 
CTGGTCGGGA CAGAGAATGG TCTGAAGCTG CTGGACAGAA GCGGTCAGGG TAAAGTGTAT 
CCACTGATCA ACTCGCGCAG ATTTCAGCAG ATGGATGTCC TGGAGGGACT GAACCTCCTC 
ATCACTATAT CAGGCAAGAA GAATAAGGTA CGCGTGTATT ATCTGGCCTG GCTGAGGAAT 
AAGATCCTCC ATAATGACCC AGAGGTGGAG AAGAAACAGG GCTGGACCAC TGTAGGGGAA 
ATGGAGGGCT GTGTGCACTA TAAAGTGGTG AAATATGAAA GGATTAAGTT TCTGGTTATC 
GCTCTGAAGA ATGCAGTGGA GGTTTACGCC TGGGCTCCAA AACCTTACCA CAAGTTCATG 
GCCTTCAAGT CATTTGGAGA CCTGCCGCAC AGACCTGTGT TGGTTGACCT CACAGTGGAA 
GAGGGTCAGA GGTTAAAGGT CATCTATGGC TCCTTGGCTG GCTTCCATGC CATTGATGTT 
GACTCTGGAA ACAACTACGA CATCTACATT CCTGTACATA TCCAGTCACA GATCACTCCT 
CACGCCATCG TGTTCCTGCC CAACTCAGAT GGGATGGAGA TGCTGTTGTG TTATGAGGAC 
GAAGGTGTTT ATGTCAACAC GTACGGACGC ATCATTAAAG ATGTGGTGCT GCAGTGGGGA 
GAAATGCCCA CATCAGTAGC CCACATTTGC TCTAATCAGA TTATGGGTTG GGGAGAGAAA 
GCCATTGAGA TCCGTTCTGT TGAGACGGGA CACCTGGATG GAGTCTTCAT GCACAAGAGA 
GCCCAGAGGC TTAAGTTCCT GTGTGAACGG AACGACAAGG TGTTTTTCGC GTCAGTGCGT 
TCCGGTGGCA GCAGCCAAGT TTACTTCATG ACCCTCAACA GAAACTGCAT TATGAACTGG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mink1 ( XM_017356007.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017211496.1 Danio rerio misshapen-like kinase 1 (mink1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa59035
Clone ID Related Accession (Same CDS sequence)	XM_017356007.1
Accession Version	XM_017356007.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3885bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	misshapen-like kinase 1 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGAAA ACGCACCTAC GCGGAGTCTG GATGACATCG ACCTGGCCGC CCTGCGGGAC 
CCAGCAGGGA TCTTCGAGTT GGTGGAGGTC GTGGGCAACG GCACCTATGG ACAAGTTTAC 
AAGGGTCGTC ATGTTAAGAC AGGTCAGCTG GCAGCCATAA AAGTCATGGA TGTGACTGAG 
GAAGAAGAGG AGGAGATTAA AGCGGAGATT AACATGCTGA AGAAGTACAG TCACCATAGA 
AATATCGCCA CCTACTATGG AGCCTTTGTG AAGAAGAGTC CTCCTGGACA CGATGACCAG 
CTCTGGTTGG TGATGGAGTT TTGTGGTGCC GGCTCAGTAA CAGACTTGGT GAAGAACACA 
AAAGGCAACT CTTTAAAAGA AGACTGGATC GCCTATATCT GCAGAGAGAT CCTTAGGGGT 
CTGTCTCACC TCCATGCTCA TAAAGTCATC CACCGAGACA TCAAGGGACA GAATGTGCTG 
CTTACAGAGA ACGCAGAGGT TAAACTGGTG GATTTTGGGG TGAGCGCACA GTTGGATCGT 
ACTGTCGGCA GAAGAAACAC TTTCATTGGC ACTCCATACT GGATGGCCCC AGAAGTCATT 
GCCTGTGATG AAAATCCCGA CTCCACCTAT GATTACAGGA GTGATATCTG GTCTCTGGGA 
ATCACTGCTA TAGAAATGGC TGAAGGAGCA CCTCCGTTGT GTGACATGCA TCCCATGCGA 
GCTTTGTTCC TTATCCCACG GAACCCTCCT CCCAAACTGA AGTCCAAGAA ATGGTCTAAG 
AAGTTCATTG ACTTCATCGA GGGCTGTCTG GTAAAGTCAT ATCCGAATCG TCCGTCCACT 
GAACAGCTCC TGAAACACTC ATTCATCAGA GATCAGCCCA CTGAAAGACA AGTCCGCATC 
CAGCTCAAAG ATCACATCGA CAGAACACGC AAGAAACGTG GCGAAAAAGA GGAGACAGAG 
TACGAGTACA GCGGCAGTGA TGAAGAGGAT GAGAACAGAG GAGATGAGAG AGAATCCAGC 
TCTATTTTGA ATGTTCCTGG TGAGTCGACG TTGCGGAGGG ATTTCCTGCG GCTGCAGCAA 
GAAAATAAAG AGCGTTCAGA GGCCTTAAAG AGACAGCAGG CGCAGCTCGC CGCTCAGCGC 
AGAGACCCTG AGGAGCATAA ACGCCAGCTG CTGCACGACC GGCAGAAGCG CATAGAGGAG 
CAGAAAGAGC AGAGGCGACG CTTAGAGGAG CAACAGAGGA AGGAGAGAGA GATGGTGCGG 
CAACAACAGG ATAAAGCTCC TCACCGGAGA CTCGATGACA TGAGACGAGA GGAGGACAGG 
AGGTTGGCAG AGAGAGAGCA GGAGTTTATA AGACATAAGT TGGAGGAAGA GCAGCGGCAG 
TTAGAGATAC TGCAGCAGCA ATTACTGCAG GAGCAGGCAT TGCTAATGGA GTATAAGCGT 
AAACAGCTGG AGGAGCAGAG ACAGTCAGAG AGGCTTCAGA GGCAGTTACA GCAGGAACAT 
GCGTATCTGG TCTCTCTACA GCAACAGCAG CAGCAGCAAG ACAAGAAACC ACAGATGTAT 
CACTACAGCA AGAACCTAGA GAACAACAAA CCAGCCTGGG CTAGAGAGGT CGAGGAGCGC 
AGTAAGCTGA ACAGACAGGG CTCTCCCAAA ATCTGCACCA CGGTCTCTGA CACTAATATT 
CAATCCCGCT CTGAGTCAAT CAACCAATCA GGAGCTGCGC AGACGCCGCC CATGCAGAGA 
CCTGTGGAGC CGCAGGGTGG ACAGGGAAAG TTCCAGATGG CCCATCTAGT GCCGTTAAAA 
CCATACGCCG CTCCAGTCCC TCGCTCTCAG TCTCTTTGTG ATCAGCCCAC TAAGACCATG 
TCCGCCTTCC CCACCCAGGA CCCCTCACCC ACACCCACAC CTCGTCCTCT ACACTCCAGA 
GAACTAGTCC GTCAAAACTC TGACCCCACC TCAGAGACTC CTGCCCCTCA GTCCCGGCCA 
ATGAGAGACG ACCGCGGACC CTGGATCCGT CTTCCTGAGA TTGAACAACC TCCTAAAATT 
CCTCAGAGAA CAGCCTCTAT AGCCACTGCA CTGAACACAA ATCTCTCCTC TGGTATCAGA 
CACCCAGTCC GAGCAAGTAA TCCAGATCTG AGTCGCAATG AGCGCTGGGA GAGAGGAGAC 
AGCATGAGCA TCATGTCCAA CCTGCCACAG ACCGGCTCTC TGGAGAGACA CCGCATACTC 
AGCTCATCTA AGATGGATTC CTCTCCAACC CTCTCTCATG ATGGCCGACA CAAACCAGGA 
GAATCCCGAA CCTCATCTAG ACCCAGCAGA CCAGCGGATC ATGGTCTGTA CTCTAAGGAG 
CGAGCAGAGG AGCAGCCGCG GCCCCCTGTG AAGGCCAACG ACTACTCCTC TTCCTCAGAG 
AGCAGTGAAA GCAGCGAAGA GAGTGAAAGT GGAGAAGGAG CAGAGGAGGA AAGCCCTACT 
GACCGCCCCC GTGATGCAGA TTCTGACTCT GTGAACACTA TGGTTGTCCA TGAAGAGGAG 
GAAGGTGAAG GAGGAGAAGA AGAACGTGCT GGAGGATATG GAGACCAAAC CATGCTTGTG 
CAGAGGACTC CAGAGAAGCG CAGTCATAAT GGATACACCA ACCTGCCTGA TGTGGTTCAG 
CCTTCTCACT CTCCCACAGA CTCGGCCTCC CACTCCTCCC CCGGGAAAGA CTCTACTTAT 
GATTATCAGT CCAGGGGATT GGTCAAGGCC TCTGGAAAAT CCTCTTTTAC TACCTTTGTG 
GACCTTGGAA TGTACCAGCC ATCAGGGGGT AGTGGAGATA ACATTTCTGT TGGAGGTCTG 
GGCTCTCGTT TTGAGCAGCT AAAGCTGGAG GTGAGGAAGG GCTCCATGGT CAATGTCAAT 
CCCACAAACA CACGTCCGGT CAGTGACACT CCTGAAATCC GCAAATACAA GAAGAGATTT 
AACTCTGAGA TCCTCTGCGC TGCTCTCTGG GGTGTGAATC TGCTGGTCGG GACAGAGAAT 
GGTCTGAAGC TGCTGGACAG AAGCGGTCAG GGTAAAGTGT ATCCACTGAT CAACTCGCGC 
AGATTTCAGC AGATGGATGT CCTGGAGGGA CTGAACCTCC TCATCACTAT ATCAGGCAAG 
AAGAATAAGG TACGCGTGTA TTATCTGGCC TGGCTGAGGA ATAAGATCCT CCATAATGAC 
CCAGAGGTGG AGAAGAAACA GGGCTGGACC ACTGTAGGGG AAATGGAGGG CTGTGTGCAC 
TATAAAGTGG TGAAATATGA AAGGATTAAG TTTCTGGTTA TCGCTCTGAA GAATGCAGTG 
GAGGTTTACG CCTGGGCTCC AAAACCTTAC CACAAGTTCA TGGCCTTCAA GTCATTTGGA 
GACCTGCCGC ACAGACCTGT GTTGGTTGAC CTCACAGTGG AAGAGGGTCA GAGGTTAAAG 
GTCATCTATG GCTCCTTGGC TGGCTTCCAT GCCATTGATG TTGACTCTGG AAACAACTAC 
GACATCTACA TTCCTGTACA TATCCAGTCA CAGATCACTC CTCACGCCAT CGTGTTCCTG 
CCCAACTCAG ATGGGATGGA GATGCTGTTG TGTTATGAGG ACGAAGGTGT TTATGTCAAC 
ACGTACGGAC GCATCATTAA AGATGTGGTG CTGCAGTGGG GAGAAATGCC CACATCAGTA 
GCCCACATTT GCTCTAATCA GATTATGGGT TGGGGAGAGA AAGCCATTGA GATCCGTTCT 
GTTGAGACGG GACACCTGGA TGGAGTCTTC ATGCACAAGA GAGCCCAGAG GCTTAAGTTC 
CTGTGTGAAC GGAACGACAA GGTGTTTTTC GCGTCAGTGC GTTCCGGTGG CAGCAGCCAA 
GTTTACTTCA TGACCCTCAA CAGAAACTGC ATTATGAACT GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017211496.1
CDS	1243..5127
Translation	MSENAPTRSLDDIDLAALRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEEEEEEIKAEINMLKKYSHHRNIATYYGAFVKKSPPGHDDQLWLVMEFCGAGSVTDLVKNTKGNSLKEDWIAYICREILRGLSHLHAHKVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDSTYDYRSDIWSLGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPKLKSKKWSKKFIDFIEGCLVKSYPNRPSTEQLLKHSFIRDQPTERQVRIQLKDHIDRTRKKRGEKEETEYEYSGSDEEDENRGDERESSSILNVPGESTLRRDFLRLQQENKERSEALKRQQAQLAAQRRDPEEHKRQLLHDRQKRIEEQKEQRRRLEEQQRKEREMVRQQQDKAPHRRLDDMRREEDRRLAEREQEFIRHKLEEEQRQLEILQQQLLQEQALLMEYKRKQLEEQRQSERLQRQLQQEHAYLVSLQQQQQQQDKKPQMYHYSKNLENNKPAWAREVEERSKLNRQGSPKICTTVSDTNIQSRSESINQSGAAQTPPMQRPVEPQGGQGKFQMAHLVPLKPYAAPVPRSQSLCDQPTKTMSAFPTQDPSPTPTPRPLHSRELVRQNSDPTSETPAPQSRPMRDDRGPWIRLPEIEQPPKIPQRTASIATALNTNLSSGIRHPVRASNPDLSRNERWERGDSMSIMSNLPQTGSLERHRILSSSKMDSSPTLSHDGRHKPGESRTSSRPSRPADHGLYSKERAEEQPRPPVKANDYSSSSESSESSEESESGEGAEEESPTDRPRDADSDSVNTMVVHEEEEGEGGEEERAGGYGDQTMLVQRTPEKRSHNGYTNLPDVVQPSHSPTDSASHSSPGKDSTYDYQSRGLVKASGKSSFTTFVDLGMYQPSGGSGDNISVGGLGSRFEQLKLEVRKGSMVNVNPTNTRPVSDTPEIRKYKKRFNSEILCAALWGVNLLVGTENGLKLLDRSGQGKVYPLINSRRFQQMDVLEGLNLLITISGKKNKVRVYYLAWLRNKILHNDPEVEKKQGWTTVGEMEGCVHYKVVKYERIKFLVIALKNAVEVYAWAPKPYHKFMAFKSFGDLPHRPVLVDLTVEEGQRLKVIYGSLAGFHAIDVDSGNNYDIYIPVHIQSQITPHAIVFLPNSDGMEMLLCYEDEGVYVNTYGRIIKDVVLQWGEMPTSVAHICSNQIMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLNRNCIMNW

Target ORF information:

RefSeq Version	XM_017356007.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio misshapen-like kinase 1 (mink1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017356007.1

ORF Insert Sequence:

ATGTCAGAAA ACGCACCTAC GCGGAGTCTG GATGACATCG ACCTGGCCGC CCTGCGGGAC 
CCAGCAGGGA TCTTCGAGTT GGTGGAGGTC GTGGGCAACG GCACCTATGG ACAAGTTTAC 
AAGGGTCGTC ATGTTAAGAC AGGTCAGCTG GCAGCCATAA AAGTCATGGA TGTGACTGAG 
GAAGAAGAGG AGGAGATTAA AGCGGAGATT AACATGCTGA AGAAGTACAG TCACCATAGA 
AATATCGCCA CCTACTATGG AGCCTTTGTG AAGAAGAGTC CTCCTGGACA CGATGACCAG 
CTCTGGTTGG TGATGGAGTT TTGTGGTGCC GGCTCAGTAA CAGACTTGGT GAAGAACACA 
AAAGGCAACT CTTTAAAAGA AGACTGGATC GCCTATATCT GCAGAGAGAT CCTTAGGGGT 
CTGTCTCACC TCCATGCTCA TAAAGTCATC CACCGAGACA TCAAGGGACA GAATGTGCTG 
CTTACAGAGA ACGCAGAGGT TAAACTGGTG GATTTTGGGG TGAGCGCACA GTTGGATCGT 
ACTGTCGGCA GAAGAAACAC TTTCATTGGC ACTCCATACT GGATGGCCCC AGAAGTCATT 
GCCTGTGATG AAAATCCCGA CTCCACCTAT GATTACAGGA GTGATATCTG GTCTCTGGGA 
ATCACTGCTA TAGAAATGGC TGAAGGAGCA CCTCCGTTGT GTGACATGCA TCCCATGCGA 
GCTTTGTTCC TTATCCCACG GAACCCTCCT CCCAAACTGA AGTCCAAGAA ATGGTCTAAG 
AAGTTCATTG ACTTCATCGA GGGCTGTCTG GTAAAGTCAT ATCCGAATCG TCCGTCCACT 
GAACAGCTCC TGAAACACTC ATTCATCAGA GATCAGCCCA CTGAAAGACA AGTCCGCATC 
CAGCTCAAAG ATCACATCGA CAGAACACGC AAGAAACGTG GCGAAAAAGA GGAGACAGAG 
TACGAGTACA GCGGCAGTGA TGAAGAGGAT GAGAACAGAG GAGATGAGAG AGAATCCAGC 
TCTATTTTGA ATGTTCCTGG TGAGTCGACG TTGCGGAGGG ATTTCCTGCG GCTGCAGCAA 
GAAAATAAAG AGCGTTCAGA GGCCTTAAAG AGACAGCAGG CGCAGCTCGC CGCTCAGCGC 
AGAGACCCTG AGGAGCATAA ACGCCAGCTG CTGCACGACC GGCAGAAGCG CATAGAGGAG 
CAGAAAGAGC AGAGGCGACG CTTAGAGGAG CAACAGAGGA AGGAGAGAGA GATGGTGCGG 
CAACAACAGG ATAAAGCTCC TCACCGGAGA CTCGATGACA TGAGACGAGA GGAGGACAGG 
AGGTTGGCAG AGAGAGAGCA GGAGTTTATA AGACATAAGT TGGAGGAAGA GCAGCGGCAG 
TTAGAGATAC TGCAGCAGCA ATTACTGCAG GAGCAGGCAT TGCTAATGGA GTATAAGCGT 
AAACAGCTGG AGGAGCAGAG ACAGTCAGAG AGGCTTCAGA GGCAGTTACA GCAGGAACAT 
GCGTATCTGG TCTCTCTACA GCAACAGCAG CAGCAGCAAG ACAAGAAACC ACAGATGTAT 
CACTACAGCA AGAACCTAGA GAACAACAAA CCAGCCTGGG CTAGAGAGGT CGAGGAGCGC 
AGTAAGCTGA ACAGACAGGG CTCTCCCAAA ATCTGCACCA CGGTCTCTGA CACTAATATT 
CAATCCCGCT CTGAGTCAAT CAACCAATCA GGAGCTGCGC AGACGCCGCC CATGCAGAGA 
CCTGTGGAGC CGCAGGGTGG ACAGGGAAAG TTCCAGATGG CCCATCTAGT GCCGTTAAAA 
CCATACGCCG CTCCAGTCCC TCGCTCTCAG TCTCTTTGTG ATCAGCCCAC TAAGACCATG 
TCCGCCTTCC CCACCCAGGA CCCCTCACCC ACACCCACAC CTCGTCCTCT ACACTCCAGA 
GAACTAGTCC GTCAAAACTC TGACCCCACC TCAGAGACTC CTGCCCCTCA GTCCCGGCCA 
ATGAGAGACG ACCGCGGACC CTGGATCCGT CTTCCTGAGA TTGAACAACC TCCTAAAATT 
CCTCAGAGAA CAGCCTCTAT AGCCACTGCA CTGAACACAA ATCTCTCCTC TGGTATCAGA 
CACCCAGTCC GAGCAAGTAA TCCAGATCTG AGTCGCAATG AGCGCTGGGA GAGAGGAGAC 
AGCATGAGCA TCATGTCCAA CCTGCCACAG ACCGGCTCTC TGGAGAGACA CCGCATACTC 
AGCTCATCTA AGATGGATTC CTCTCCAACC CTCTCTCATG ATGGCCGACA CAAACCAGGA 
GAATCCCGAA CCTCATCTAG ACCCAGCAGA CCAGCGGATC ATGGTCTGTA CTCTAAGGAG 
CGAGCAGAGG AGCAGCCGCG GCCCCCTGTG AAGGCCAACG ACTACTCCTC TTCCTCAGAG 
AGCAGTGAAA GCAGCGAAGA GAGTGAAAGT GGAGAAGGAG CAGAGGAGGA AAGCCCTACT 
GACCGCCCCC GTGATGCAGA TTCTGACTCT GTGAACACTA TGGTTGTCCA TGAAGAGGAG 
GAAGGTGAAG GAGGAGAAGA AGAACGTGCT GGAGGATATG GAGACCAAAC CATGCTTGTG 
CAGAGGACTC CAGAGAAGCG CAGTCATAAT GGATACACCA ACCTGCCTGA TGTGGTTCAG 
CCTTCTCACT CTCCCACAGA CTCGGCCTCC CACTCCTCCC CCGGGAAAGA CTCTACTTAT 
GATTATCAGT CCAGGGGATT GGTCAAGGCC TCTGGAAAAT CCTCTTTTAC TACCTTTGTG 
GACCTTGGAA TGTACCAGCC ATCAGGGGGT AGTGGAGATA ACATTTCTGT TGGAGGTCTG 
GGCTCTCGTT TTGAGCAGCT AAAGCTGGAG GTGAGGAAGG GCTCCATGGT CAATGTCAAT 
CCCACAAACA CACGTCCGGT CAGTGACACT CCTGAAATCC GCAAATACAA GAAGAGATTT 
AACTCTGAGA TCCTCTGCGC TGCTCTCTGG GGTGTGAATC TGCTGGTCGG GACAGAGAAT 
GGTCTGAAGC TGCTGGACAG AAGCGGTCAG GGTAAAGTGT ATCCACTGAT CAACTCGCGC 
AGATTTCAGC AGATGGATGT CCTGGAGGGA CTGAACCTCC TCATCACTAT ATCAGGCAAG 
AAGAATAAGG TACGCGTGTA TTATCTGGCC TGGCTGAGGA ATAAGATCCT CCATAATGAC 
CCAGAGGTGG AGAAGAAACA GGGCTGGACC ACTGTAGGGG AAATGGAGGG CTGTGTGCAC 
TATAAAGTGG TGAAATATGA AAGGATTAAG TTTCTGGTTA TCGCTCTGAA GAATGCAGTG 
GAGGTTTACG CCTGGGCTCC AAAACCTTAC CACAAGTTCA TGGCCTTCAA GTCATTTGGA 
GACCTGCCGC ACAGACCTGT GTTGGTTGAC CTCACAGTGG AAGAGGGTCA GAGGTTAAAG 
GTCATCTATG GCTCCTTGGC TGGCTTCCAT GCCATTGATG TTGACTCTGG AAACAACTAC 
GACATCTACA TTCCTGTACA TATCCAGTCA CAGATCACTC CTCACGCCAT CGTGTTCCTG 
CCCAACTCAG ATGGGATGGA GATGCTGTTG TGTTATGAGG ACGAAGGTGT TTATGTCAAC 
ACGTACGGAC GCATCATTAA AGATGTGGTG CTGCAGTGGG GAGAAATGCC CACATCAGTA 
GCCCACATTT GCTCTAATCA GATTATGGGT TGGGGAGAGA AAGCCATTGA GATCCGTTCT 
GTTGAGACGG GACACCTGGA TGGAGTCTTC ATGCACAAGA GAGCCCAGAG GCTTAAGTTC 
CTGTGTGAAC GGAACGACAA GGTGTTTTTC GCGTCAGTGC GTTCCGGTGG CAGCAGCCAA 
GTTTACTTCA TGACCCTCAA CAGAAACTGC ATTATGAACT GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mink1 ( XM_017356008.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017211497.1 Danio rerio misshapen-like kinase 1 (mink1), transcript variant X7, mRNA.

Price & Availability↑

CloneID	ODa59036
Clone ID Related Accession (Same CDS sequence)	XM_017356008.2 , XM_017356008.1
Accession Version	XM_017356008.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3816bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	misshapen-like kinase 1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017356008.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGAAA ACGCACCTAC GCGGAGTCTG GATGACATCG ACCTGGCCGC CCTGCGGGAC 
CCAGCAGGGA TCTTCGAGTT GGTGGAGGTC GTGGGCAACG GCACCTATGG ACAAGTTTAC 
AAGGGTCGTC ATGTTAAGAC AGGTCAGCTG GCAGCCATAA AAGTCATGGA TGTGACTGAG 
GAAGAAGAGG AGGAGATTAA AGCGGAGATT AACATGCTGA AGAAGTACAG TCACCATAGA 
AATATCGCCA CCTACTATGG AGCCTTTGTG AAGAAGAGTC CTCCTGGACA CGATGACCAG 
CTCTGGTTGG TGATGGAGTT TTGTGGTGCC GGCTCAGTAA CAGACTTGGT GAAGAACACA 
AAAGGCAACT CTTTAAAAGA AGACTGGATC GCCTATATCT GCAGAGAGAT CCTTAGGGGT 
CTGTCTCACC TCCATGCTCA TAAAGTCATC CACCGAGACA TCAAGGGACA GAATGTGCTG 
CTTACAGAGA ACGCAGAGGT TAAACTGGTG GATTTTGGGG TGAGCGCACA GTTGGATCGT 
ACTGTCGGCA GAAGAAACAC TTTCATTGGC ACTCCATACT GGATGGCCCC AGAAGTCATT 
GCCTGTGATG AAAATCCCGA CTCCACCTAT GATTACAGGA GTGATATCTG GTCTCTGGGA 
ATCACTGCTA TAGAAATGGC TGAAGGAGCA CCTCCGTTGT GTGACATGCA TCCCATGCGA 
GCTTTGTTCC TTATCCCACG GAACCCTCCT CCCAAACTGA AGTCCAAGAA ATGGTCTAAG 
AAGTTCATTG ACTTCATCGA GGGCTGTCTG GTAAAGTCAT ATCCGAATCG TCCGTCCACT 
GAACAGCTCC TGAAACACTC ATTCATCAGA GATCAGCCCA CTGAAAGACA AGTCCGCATC 
CAGCTCAAAG ATCACATCGA CAGAACACGC AAGAAACGTG GCGAAAAAGA GGAGACAGAG 
TACGAGTACA GCGGCAGTGA TGAAGAGGAT GAGAACAGAG GAGATGAGAG AGAATCCAGC 
TCTATTTTGA ATGTTCCTGG TGAGTCGACG TTGCGGAGGG ATTTCCTGCG GCTGCAGCAA 
GAAAATAAAG AGCGTTCAGA GGCCTTAAAG AGACAGCAGG CGCAGCTCGC CGCTCAGCGC 
AGAGACCCTG AGGAGCATAA ACGCCAGCTG CTGCACGACC GGCAGAAGCG CATAGAGGAG 
CAGAAAGAGC AGAGGCGACG CTTAGAGGAG CAACAGAGGA AGGAGAGAGA GATGGTGCGG 
CAACAACAGG ATAAAGCTCC TCACCGGAGA CTCGATGACA TGAGACGAGA GGAGGACAGG 
AGGTTGGCAG AGAGAGAGCA GGAGTATAAG CGTAAACAGC TGGAGGAGCA GAGACAGTCA 
GAGAGGCTTC AGAGGCAGTT ACAGCAGGAA CATGCGTATC TGGTCTCTCT ACAGCAACAG 
CAGCAGCAGC AAGACAAGAA ACCACAGATG TATCACTACA GCAAGAACCT AGAGAACAAC 
AAACCAGCCT GGGCTAGAGA GGTCGAGGAG CGCAGTAAGC TGAACAGACA GGGCTCTCCC 
AAAATCTGCA CCACGGTCTC TGACACTAAT ATTCAATCCC GCTCTGAGTC AATCAACCAA 
TCAGGAGCTG CGCAGACGCC GCCCATGCAG AGACCTGTGG AGCCGCAGGG TGGACAGGGA 
AAGATGGCCC ATCTAGTGCC GTTAAAACCA TACGCCGCTC CAGTCCCTCG CTCTCAGTCT 
CTTTGTGATC AGCCCACTAA GACCATGTCC GCCTTCCCCA CCCAGGACCC CTCACCCACA 
CCCACACCTC GTCCTCTACA CTCCAGAGAA CTAGTCCGTC AAAACTCTGA CCCCACCTCA 
GAGACTCCTG CCCCTCAGTC CCGGCCAATG AGAGACGACC GCGGACCCTG GATCCGTCTT 
CCTGAGATTG AACAACCTCC TAAAATTCCT CAGAGAACAG CCTCTATAGC CACTGCACTG 
AACACAAATC TCTCCTCTGG TATCAGACAC CCAGTCCGAG CAAGTAATCC AGATCTGAGT 
CGCAATGAGC GCTGGGAGAG AGGAGACAGC ATGAGCATCA TGTCCAACCT GCCACAGACC 
GGCTCTCTGG AGAGACACCG CATACTCAGC TCATCTAAGA TGGATTCCTC TCCAACCCTC 
TCTCATGATG GCCGACACAA ACCAGGAGAA TCCCGAACCT CATCTAGACC CAGCAGACCA 
GCGAGCTATA AGAGAGCAAT AGGAGAGGAT CATGGTCTGT ACTCTAAGGA GCGAGCAGAG 
GAGCAGCCGC GGCCCCCTGT GAAGGCCAAC GACTACTCCT CTTCCTCAGA GAGCAGTGAA 
AGCAGCGAAG AGAGTGAAAG TGGAGAAGGA GCAGAGGAGG AAAGCCCTAC TGACCGCCCC 
CGTGATGCAG ATTCTGACTC TGTGAACACT ATGGTTGTCC ATGAAGAGGA GGAAGGTGAA 
GGAGGAGAAG AAGAACGTGC TGGAGGATAT GGAGACCAAA CCATGCTTGT GCAGAGGACT 
CCAGAGAAGC GCAGTCATAA TGGATACACC AACCTGCCTG ATGTGGTTCA GCCTTCTCAC 
TCTCCCACAG ACTCGGCCTC CCACTCCTCC CCCGGGAAAG ACTCTACTTA TGATTATCAG 
TCCAGGGGAT TGGTCAAGGC CTCTGGAAAA TCCTCTTTTA CTACCTTTGT GGACCTTGGA 
ATGTACCAGC CATCAGGGGG TAGTGGAGAT AACATTTCTG TTGGAGGTCT GGGCTCTCGT 
TTTGAGCAGC TAAAGCTGGA GGTGAGGAAG GGCTCCATGG TCAATGTCAA TCCCACAAAC 
ACACGTCCGG TCAGTGACAC TCCTGAAATC CGCAAATACA AGAAGAGATT TAACTCTGAG 
ATCCTCTGCG CTGCTCTCTG GGGTGTGAAT CTGCTGGTCG GGACAGAGAA TGGTCTGAAG 
CTGCTGGACA GAAGCGGTCA GGGTAAAGTG TATCCACTGA TCAACTCGCG CAGATTTCAG 
CAGATGGATG TCCTGGAGGG ACTGAACCTC CTCATCACTA TATCAGGCAA GAAGAATAAG 
GTACGCGTGT ATTATCTGGC CTGGCTGAGG AATAAGATCC TCCATAATGA CCCAGAGGTG 
GAGAAGAAAC AGGGCTGGAC CACTGTAGGG GAAATGGAGG GCTGTGTGCA CTATAAAGTG 
GTGAAATATG AAAGGATTAA GTTTCTGGTT ATCGCTCTGA AGAATGCAGT GGAGGTTTAC 
GCCTGGGCTC CAAAACCTTA CCACAAGTTC ATGGCCTTCA AGTCATTTGG AGACCTGCCG 
CACAGACCTG TGTTGGTTGA CCTCACAGTG GAAGAGGGTC AGAGGTTAAA GGTCATCTAT 
GGCTCCTTGG CTGGCTTCCA TGCCATTGAT GTTGACTCTG GAAACAACTA CGACATCTAC 
ATTCCTGTAC ATATCCAGTC ACAGATCACT CCTCACGCCA TCGTGTTCCT GCCCAACTCA 
GATGGGATGG AGATGCTGTT GTGTTATGAG GACGAAGGTG TTTATGTCAA CACGTACGGA 
CGCATCATTA AAGATGTGGT GCTGCAGTGG GGAGAAATGC CCACATCAGT AGCCCACATT 
TGCTCTAATC AGATTATGGG TTGGGGAGAG AAAGCCATTG AGATCCGTTC TGTTGAGACG 
GGACACCTGG ATGGAGTCTT CATGCACAAG AGAGCCCAGA GGCTTAAGTT CCTGTGTGAA 
CGGAACGACA AGGTGTTTTT CGCGTCAGTG CGTTCCGGTG GCAGCAGCCA AGTTTACTTC 
ATGACCCTCA ACAGAAACTG CATTATGAAC TGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017211497.1
CDS	1262..5077
Translation	MSENAPTRSLDDIDLAALRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEEEEEEIKAEINMLKKYSHHRNIATYYGAFVKKSPPGHDDQLWLVMEFCGAGSVTDLVKNTKGNSLKEDWIAYICREILRGLSHLHAHKVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDSTYDYRSDIWSLGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPKLKSKKWSKKFIDFIEGCLVKSYPNRPSTEQLLKHSFIRDQPTERQVRIQLKDHIDRTRKKRGEKEETEYEYSGSDEEDENRGDERESSSILNVPGESTLRRDFLRLQQENKERSEALKRQQAQLAAQRRDPEEHKRQLLHDRQKRIEEQKEQRRRLEEQQRKEREMVRQQQDKAPHRRLDDMRREEDRRLAEREQEYKRKQLEEQRQSERLQRQLQQEHAYLVSLQQQQQQQDKKPQMYHYSKNLENNKPAWAREVEERSKLNRQGSPKICTTVSDTNIQSRSESINQSGAAQTPPMQRPVEPQGGQGKMAHLVPLKPYAAPVPRSQSLCDQPTKTMSAFPTQDPSPTPTPRPLHSRELVRQNSDPTSETPAPQSRPMRDDRGPWIRLPEIEQPPKIPQRTASIATALNTNLSSGIRHPVRASNPDLSRNERWERGDSMSIMSNLPQTGSLERHRILSSSKMDSSPTLSHDGRHKPGESRTSSRPSRPASYKRAIGEDHGLYSKERAEEQPRPPVKANDYSSSSESSESSEESESGEGAEEESPTDRPRDADSDSVNTMVVHEEEEGEGGEEERAGGYGDQTMLVQRTPEKRSHNGYTNLPDVVQPSHSPTDSASHSSPGKDSTYDYQSRGLVKASGKSSFTTFVDLGMYQPSGGSGDNISVGGLGSRFEQLKLEVRKGSMVNVNPTNTRPVSDTPEIRKYKKRFNSEILCAALWGVNLLVGTENGLKLLDRSGQGKVYPLINSRRFQQMDVLEGLNLLITISGKKNKVRVYYLAWLRNKILHNDPEVEKKQGWTTVGEMEGCVHYKVVKYERIKFLVIALKNAVEVYAWAPKPYHKFMAFKSFGDLPHRPVLVDLTVEEGQRLKVIYGSLAGFHAIDVDSGNNYDIYIPVHIQSQITPHAIVFLPNSDGMEMLLCYEDEGVYVNTYGRIIKDVVLQWGEMPTSVAHICSNQIMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLNRNCIMNW

Target ORF information:

RefSeq Version	XM_017356008.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio misshapen-like kinase 1 (mink1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017356008.2

ORF Insert Sequence:

ATGTCAGAAA ACGCACCTAC GCGGAGTCTG GATGACATCG ACCTGGCCGC CCTGCGGGAC 
CCAGCAGGGA TCTTCGAGTT GGTGGAGGTC GTGGGCAACG GCACCTATGG ACAAGTTTAC 
AAGGGTCGTC ATGTTAAGAC AGGTCAGCTG GCAGCCATAA AAGTCATGGA TGTGACTGAG 
GAAGAAGAGG AGGAGATTAA AGCGGAGATT AACATGCTGA AGAAGTACAG TCACCATAGA 
AATATCGCCA CCTACTATGG AGCCTTTGTG AAGAAGAGTC CTCCTGGACA CGATGACCAG 
CTCTGGTTGG TGATGGAGTT TTGTGGTGCC GGCTCAGTAA CAGACTTGGT GAAGAACACA 
AAAGGCAACT CTTTAAAAGA AGACTGGATC GCCTATATCT GCAGAGAGAT CCTTAGGGGT 
CTGTCTCACC TCCATGCTCA TAAAGTCATC CACCGAGACA TCAAGGGACA GAATGTGCTG 
CTTACAGAGA ACGCAGAGGT TAAACTGGTG GATTTTGGGG TGAGCGCACA GTTGGATCGT 
ACTGTCGGCA GAAGAAACAC TTTCATTGGC ACTCCATACT GGATGGCCCC AGAAGTCATT 
GCCTGTGATG AAAATCCCGA CTCCACCTAT GATTACAGGA GTGATATCTG GTCTCTGGGA 
ATCACTGCTA TAGAAATGGC TGAAGGAGCA CCTCCGTTGT GTGACATGCA TCCCATGCGA 
GCTTTGTTCC TTATCCCACG GAACCCTCCT CCCAAACTGA AGTCCAAGAA ATGGTCTAAG 
AAGTTCATTG ACTTCATCGA GGGCTGTCTG GTAAAGTCAT ATCCGAATCG TCCGTCCACT 
GAACAGCTCC TGAAACACTC ATTCATCAGA GATCAGCCCA CTGAAAGACA AGTCCGCATC 
CAGCTCAAAG ATCACATCGA CAGAACACGC AAGAAACGTG GCGAAAAAGA GGAGACAGAG 
TACGAGTACA GCGGCAGTGA TGAAGAGGAT GAGAACAGAG GAGATGAGAG AGAATCCAGC 
TCTATTTTGA ATGTTCCTGG TGAGTCGACG TTGCGGAGGG ATTTCCTGCG GCTGCAGCAA 
GAAAATAAAG AGCGTTCAGA GGCCTTAAAG AGACAGCAGG CGCAGCTCGC CGCTCAGCGC 
AGAGACCCTG AGGAGCATAA ACGCCAGCTG CTGCACGACC GGCAGAAGCG CATAGAGGAG 
CAGAAAGAGC AGAGGCGACG CTTAGAGGAG CAACAGAGGA AGGAGAGAGA GATGGTGCGG 
CAACAACAGG ATAAAGCTCC TCACCGGAGA CTCGATGACA TGAGACGAGA GGAGGACAGG 
AGGTTGGCAG AGAGAGAGCA GGAGTATAAG CGTAAACAGC TGGAGGAGCA GAGACAGTCA 
GAGAGGCTTC AGAGGCAGTT ACAGCAGGAA CATGCGTATC TGGTCTCTCT ACAGCAACAG 
CAGCAGCAGC AAGACAAGAA ACCACAGATG TATCACTACA GCAAGAACCT AGAGAACAAC 
AAACCAGCCT GGGCTAGAGA GGTCGAGGAG CGCAGTAAGC TGAACAGACA GGGCTCTCCC 
AAAATCTGCA CCACGGTCTC TGACACTAAT ATTCAATCCC GCTCTGAGTC AATCAACCAA 
TCAGGAGCTG CGCAGACGCC GCCCATGCAG AGACCTGTGG AGCCGCAGGG TGGACAGGGA 
AAGATGGCCC ATCTAGTGCC GTTAAAACCA TACGCCGCTC CAGTCCCTCG CTCTCAGTCT 
CTTTGTGATC AGCCCACTAA GACCATGTCC GCCTTCCCCA CCCAGGACCC CTCACCCACA 
CCCACACCTC GTCCTCTACA CTCCAGAGAA CTAGTCCGTC AAAACTCTGA CCCCACCTCA 
GAGACTCCTG CCCCTCAGTC CCGGCCAATG AGAGACGACC GCGGACCCTG GATCCGTCTT 
CCTGAGATTG AACAACCTCC TAAAATTCCT CAGAGAACAG CCTCTATAGC CACTGCACTG 
AACACAAATC TCTCCTCTGG TATCAGACAC CCAGTCCGAG CAAGTAATCC AGATCTGAGT 
CGCAATGAGC GCTGGGAGAG AGGAGACAGC ATGAGCATCA TGTCCAACCT GCCACAGACC 
GGCTCTCTGG AGAGACACCG CATACTCAGC TCATCTAAGA TGGATTCCTC TCCAACCCTC 
TCTCATGATG GCCGACACAA ACCAGGAGAA TCCCGAACCT CATCTAGACC CAGCAGACCA 
GCGAGCTATA AGAGAGCAAT AGGAGAGGAT CATGGTCTGT ACTCTAAGGA GCGAGCAGAG 
GAGCAGCCGC GGCCCCCTGT GAAGGCCAAC GACTACTCCT CTTCCTCAGA GAGCAGTGAA 
AGCAGCGAAG AGAGTGAAAG TGGAGAAGGA GCAGAGGAGG AAAGCCCTAC TGACCGCCCC 
CGTGATGCAG ATTCTGACTC TGTGAACACT ATGGTTGTCC ATGAAGAGGA GGAAGGTGAA 
GGAGGAGAAG AAGAACGTGC TGGAGGATAT GGAGACCAAA CCATGCTTGT GCAGAGGACT 
CCAGAGAAGC GCAGTCATAA TGGATACACC AACCTGCCTG ATGTGGTTCA GCCTTCTCAC 
TCTCCCACAG ACTCGGCCTC CCACTCCTCC CCCGGGAAAG ACTCTACTTA TGATTATCAG 
TCCAGGGGAT TGGTCAAGGC CTCTGGAAAA TCCTCTTTTA CTACCTTTGT GGACCTTGGA 
ATGTACCAGC CATCAGGGGG TAGTGGAGAT AACATTTCTG TTGGAGGTCT GGGCTCTCGT 
TTTGAGCAGC TAAAGCTGGA GGTGAGGAAG GGCTCCATGG TCAATGTCAA TCCCACAAAC 
ACACGTCCGG TCAGTGACAC TCCTGAAATC CGCAAATACA AGAAGAGATT TAACTCTGAG 
ATCCTCTGCG CTGCTCTCTG GGGTGTGAAT CTGCTGGTCG GGACAGAGAA TGGTCTGAAG 
CTGCTGGACA GAAGCGGTCA GGGTAAAGTG TATCCACTGA TCAACTCGCG CAGATTTCAG 
CAGATGGATG TCCTGGAGGG ACTGAACCTC CTCATCACTA TATCAGGCAA GAAGAATAAG 
GTACGCGTGT ATTATCTGGC CTGGCTGAGG AATAAGATCC TCCATAATGA CCCAGAGGTG 
GAGAAGAAAC AGGGCTGGAC CACTGTAGGG GAAATGGAGG GCTGTGTGCA CTATAAAGTG 
GTGAAATATG AAAGGATTAA GTTTCTGGTT ATCGCTCTGA AGAATGCAGT GGAGGTTTAC 
GCCTGGGCTC CAAAACCTTA CCACAAGTTC ATGGCCTTCA AGTCATTTGG AGACCTGCCG 
CACAGACCTG TGTTGGTTGA CCTCACAGTG GAAGAGGGTC AGAGGTTAAA GGTCATCTAT 
GGCTCCTTGG CTGGCTTCCA TGCCATTGAT GTTGACTCTG GAAACAACTA CGACATCTAC 
ATTCCTGTAC ATATCCAGTC ACAGATCACT CCTCACGCCA TCGTGTTCCT GCCCAACTCA 
GATGGGATGG AGATGCTGTT GTGTTATGAG GACGAAGGTG TTTATGTCAA CACGTACGGA 
CGCATCATTA AAGATGTGGT GCTGCAGTGG GGAGAAATGC CCACATCAGT AGCCCACATT 
TGCTCTAATC AGATTATGGG TTGGGGAGAG AAAGCCATTG AGATCCGTTC TGTTGAGACG 
GGACACCTGG ATGGAGTCTT CATGCACAAG AGAGCCCAGA GGCTTAAGTT CCTGTGTGAA 
CGGAACGACA AGGTGTTTTT CGCGTCAGTG CGTTCCGGTG GCAGCAGCCA AGTTTACTTC 
ATGACCCTCA ACAGAAACTG CATTATGAAC TGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mink1 ( XM_017356008.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017211497.1 Danio rerio misshapen-like kinase 1 (mink1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ODa59036
Clone ID Related Accession (Same CDS sequence)	XM_017356008.2 , XM_017356008.1
Accession Version	XM_017356008.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3816bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	misshapen-like kinase 1 isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGAAA ACGCACCTAC GCGGAGTCTG GATGACATCG ACCTGGCCGC CCTGCGGGAC 
CCAGCAGGGA TCTTCGAGTT GGTGGAGGTC GTGGGCAACG GCACCTATGG ACAAGTTTAC 
AAGGGTCGTC ATGTTAAGAC AGGTCAGCTG GCAGCCATAA AAGTCATGGA TGTGACTGAG 
GAAGAAGAGG AGGAGATTAA AGCGGAGATT AACATGCTGA AGAAGTACAG TCACCATAGA 
AATATCGCCA CCTACTATGG AGCCTTTGTG AAGAAGAGTC CTCCTGGACA CGATGACCAG 
CTCTGGTTGG TGATGGAGTT TTGTGGTGCC GGCTCAGTAA CAGACTTGGT GAAGAACACA 
AAAGGCAACT CTTTAAAAGA AGACTGGATC GCCTATATCT GCAGAGAGAT CCTTAGGGGT 
CTGTCTCACC TCCATGCTCA TAAAGTCATC CACCGAGACA TCAAGGGACA GAATGTGCTG 
CTTACAGAGA ACGCAGAGGT TAAACTGGTG GATTTTGGGG TGAGCGCACA GTTGGATCGT 
ACTGTCGGCA GAAGAAACAC TTTCATTGGC ACTCCATACT GGATGGCCCC AGAAGTCATT 
GCCTGTGATG AAAATCCCGA CTCCACCTAT GATTACAGGA GTGATATCTG GTCTCTGGGA 
ATCACTGCTA TAGAAATGGC TGAAGGAGCA CCTCCGTTGT GTGACATGCA TCCCATGCGA 
GCTTTGTTCC TTATCCCACG GAACCCTCCT CCCAAACTGA AGTCCAAGAA ATGGTCTAAG 
AAGTTCATTG ACTTCATCGA GGGCTGTCTG GTAAAGTCAT ATCCGAATCG TCCGTCCACT 
GAACAGCTCC TGAAACACTC ATTCATCAGA GATCAGCCCA CTGAAAGACA AGTCCGCATC 
CAGCTCAAAG ATCACATCGA CAGAACACGC AAGAAACGTG GCGAAAAAGA GGAGACAGAG 
TACGAGTACA GCGGCAGTGA TGAAGAGGAT GAGAACAGAG GAGATGAGAG AGAATCCAGC 
TCTATTTTGA ATGTTCCTGG TGAGTCGACG TTGCGGAGGG ATTTCCTGCG GCTGCAGCAA 
GAAAATAAAG AGCGTTCAGA GGCCTTAAAG AGACAGCAGG CGCAGCTCGC CGCTCAGCGC 
AGAGACCCTG AGGAGCATAA ACGCCAGCTG CTGCACGACC GGCAGAAGCG CATAGAGGAG 
CAGAAAGAGC AGAGGCGACG CTTAGAGGAG CAACAGAGGA AGGAGAGAGA GATGGTGCGG 
CAACAACAGG ATAAAGCTCC TCACCGGAGA CTCGATGACA TGAGACGAGA GGAGGACAGG 
AGGTTGGCAG AGAGAGAGCA GGAGTATAAG CGTAAACAGC TGGAGGAGCA GAGACAGTCA 
GAGAGGCTTC AGAGGCAGTT ACAGCAGGAA CATGCGTATC TGGTCTCTCT ACAGCAACAG 
CAGCAGCAGC AAGACAAGAA ACCACAGATG TATCACTACA GCAAGAACCT AGAGAACAAC 
AAACCAGCCT GGGCTAGAGA GGTCGAGGAG CGCAGTAAGC TGAACAGACA GGGCTCTCCC 
AAAATCTGCA CCACGGTCTC TGACACTAAT ATTCAATCCC GCTCTGAGTC AATCAACCAA 
TCAGGAGCTG CGCAGACGCC GCCCATGCAG AGACCTGTGG AGCCGCAGGG TGGACAGGGA 
AAGATGGCCC ATCTAGTGCC GTTAAAACCA TACGCCGCTC CAGTCCCTCG CTCTCAGTCT 
CTTTGTGATC AGCCCACTAA GACCATGTCC GCCTTCCCCA CCCAGGACCC CTCACCCACA 
CCCACACCTC GTCCTCTACA CTCCAGAGAA CTAGTCCGTC AAAACTCTGA CCCCACCTCA 
GAGACTCCTG CCCCTCAGTC CCGGCCAATG AGAGACGACC GCGGACCCTG GATCCGTCTT 
CCTGAGATTG AACAACCTCC TAAAATTCCT CAGAGAACAG CCTCTATAGC CACTGCACTG 
AACACAAATC TCTCCTCTGG TATCAGACAC CCAGTCCGAG CAAGTAATCC AGATCTGAGT 
CGCAATGAGC GCTGGGAGAG AGGAGACAGC ATGAGCATCA TGTCCAACCT GCCACAGACC 
GGCTCTCTGG AGAGACACCG CATACTCAGC TCATCTAAGA TGGATTCCTC TCCAACCCTC 
TCTCATGATG GCCGACACAA ACCAGGAGAA TCCCGAACCT CATCTAGACC CAGCAGACCA 
GCGAGCTATA AGAGAGCAAT AGGAGAGGAT CATGGTCTGT ACTCTAAGGA GCGAGCAGAG 
GAGCAGCCGC GGCCCCCTGT GAAGGCCAAC GACTACTCCT CTTCCTCAGA GAGCAGTGAA 
AGCAGCGAAG AGAGTGAAAG TGGAGAAGGA GCAGAGGAGG AAAGCCCTAC TGACCGCCCC 
CGTGATGCAG ATTCTGACTC TGTGAACACT ATGGTTGTCC ATGAAGAGGA GGAAGGTGAA 
GGAGGAGAAG AAGAACGTGC TGGAGGATAT GGAGACCAAA CCATGCTTGT GCAGAGGACT 
CCAGAGAAGC GCAGTCATAA TGGATACACC AACCTGCCTG ATGTGGTTCA GCCTTCTCAC 
TCTCCCACAG ACTCGGCCTC CCACTCCTCC CCCGGGAAAG ACTCTACTTA TGATTATCAG 
TCCAGGGGAT TGGTCAAGGC CTCTGGAAAA TCCTCTTTTA CTACCTTTGT GGACCTTGGA 
ATGTACCAGC CATCAGGGGG TAGTGGAGAT AACATTTCTG TTGGAGGTCT GGGCTCTCGT 
TTTGAGCAGC TAAAGCTGGA GGTGAGGAAG GGCTCCATGG TCAATGTCAA TCCCACAAAC 
ACACGTCCGG TCAGTGACAC TCCTGAAATC CGCAAATACA AGAAGAGATT TAACTCTGAG 
ATCCTCTGCG CTGCTCTCTG GGGTGTGAAT CTGCTGGTCG GGACAGAGAA TGGTCTGAAG 
CTGCTGGACA GAAGCGGTCA GGGTAAAGTG TATCCACTGA TCAACTCGCG CAGATTTCAG 
CAGATGGATG TCCTGGAGGG ACTGAACCTC CTCATCACTA TATCAGGCAA GAAGAATAAG 
GTACGCGTGT ATTATCTGGC CTGGCTGAGG AATAAGATCC TCCATAATGA CCCAGAGGTG 
GAGAAGAAAC AGGGCTGGAC CACTGTAGGG GAAATGGAGG GCTGTGTGCA CTATAAAGTG 
GTGAAATATG AAAGGATTAA GTTTCTGGTT ATCGCTCTGA AGAATGCAGT GGAGGTTTAC 
GCCTGGGCTC CAAAACCTTA CCACAAGTTC ATGGCCTTCA AGTCATTTGG AGACCTGCCG 
CACAGACCTG TGTTGGTTGA CCTCACAGTG GAAGAGGGTC AGAGGTTAAA GGTCATCTAT 
GGCTCCTTGG CTGGCTTCCA TGCCATTGAT GTTGACTCTG GAAACAACTA CGACATCTAC 
ATTCCTGTAC ATATCCAGTC ACAGATCACT CCTCACGCCA TCGTGTTCCT GCCCAACTCA 
GATGGGATGG AGATGCTGTT GTGTTATGAG GACGAAGGTG TTTATGTCAA CACGTACGGA 
CGCATCATTA AAGATGTGGT GCTGCAGTGG GGAGAAATGC CCACATCAGT AGCCCACATT 
TGCTCTAATC AGATTATGGG TTGGGGAGAG AAAGCCATTG AGATCCGTTC TGTTGAGACG 
GGACACCTGG ATGGAGTCTT CATGCACAAG AGAGCCCAGA GGCTTAAGTT CCTGTGTGAA 
CGGAACGACA AGGTGTTTTT CGCGTCAGTG CGTTCCGGTG GCAGCAGCCA AGTTTACTTC 
ATGACCCTCA ACAGAAACTG CATTATGAAC TGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017211497.1
CDS	1245..5060
Translation	MSENAPTRSLDDIDLAALRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEEEEEEIKAEINMLKKYSHHRNIATYYGAFVKKSPPGHDDQLWLVMEFCGAGSVTDLVKNTKGNSLKEDWIAYICREILRGLSHLHAHKVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDSTYDYRSDIWSLGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPKLKSKKWSKKFIDFIEGCLVKSYPNRPSTEQLLKHSFIRDQPTERQVRIQLKDHIDRTRKKRGEKEETEYEYSGSDEEDENRGDERESSSILNVPGESTLRRDFLRLQQENKERSEALKRQQAQLAAQRRDPEEHKRQLLHDRQKRIEEQKEQRRRLEEQQRKEREMVRQQQDKAPHRRLDDMRREEDRRLAEREQEYKRKQLEEQRQSERLQRQLQQEHAYLVSLQQQQQQQDKKPQMYHYSKNLENNKPAWAREVEERSKLNRQGSPKICTTVSDTNIQSRSESINQSGAAQTPPMQRPVEPQGGQGKMAHLVPLKPYAAPVPRSQSLCDQPTKTMSAFPTQDPSPTPTPRPLHSRELVRQNSDPTSETPAPQSRPMRDDRGPWIRLPEIEQPPKIPQRTASIATALNTNLSSGIRHPVRASNPDLSRNERWERGDSMSIMSNLPQTGSLERHRILSSSKMDSSPTLSHDGRHKPGESRTSSRPSRPASYKRAIGEDHGLYSKERAEEQPRPPVKANDYSSSSESSESSEESESGEGAEEESPTDRPRDADSDSVNTMVVHEEEEGEGGEEERAGGYGDQTMLVQRTPEKRSHNGYTNLPDVVQPSHSPTDSASHSSPGKDSTYDYQSRGLVKASGKSSFTTFVDLGMYQPSGGSGDNISVGGLGSRFEQLKLEVRKGSMVNVNPTNTRPVSDTPEIRKYKKRFNSEILCAALWGVNLLVGTENGLKLLDRSGQGKVYPLINSRRFQQMDVLEGLNLLITISGKKNKVRVYYLAWLRNKILHNDPEVEKKQGWTTVGEMEGCVHYKVVKYERIKFLVIALKNAVEVYAWAPKPYHKFMAFKSFGDLPHRPVLVDLTVEEGQRLKVIYGSLAGFHAIDVDSGNNYDIYIPVHIQSQITPHAIVFLPNSDGMEMLLCYEDEGVYVNTYGRIIKDVVLQWGEMPTSVAHICSNQIMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLNRNCIMNW

Target ORF information:

RefSeq Version	XM_017356008.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio misshapen-like kinase 1 (mink1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017356008.1

ORF Insert Sequence:

ATGTCAGAAA ACGCACCTAC GCGGAGTCTG GATGACATCG ACCTGGCCGC CCTGCGGGAC 
CCAGCAGGGA TCTTCGAGTT GGTGGAGGTC GTGGGCAACG GCACCTATGG ACAAGTTTAC 
AAGGGTCGTC ATGTTAAGAC AGGTCAGCTG GCAGCCATAA AAGTCATGGA TGTGACTGAG 
GAAGAAGAGG AGGAGATTAA AGCGGAGATT AACATGCTGA AGAAGTACAG TCACCATAGA 
AATATCGCCA CCTACTATGG AGCCTTTGTG AAGAAGAGTC CTCCTGGACA CGATGACCAG 
CTCTGGTTGG TGATGGAGTT TTGTGGTGCC GGCTCAGTAA CAGACTTGGT GAAGAACACA 
AAAGGCAACT CTTTAAAAGA AGACTGGATC GCCTATATCT GCAGAGAGAT CCTTAGGGGT 
CTGTCTCACC TCCATGCTCA TAAAGTCATC CACCGAGACA TCAAGGGACA GAATGTGCTG 
CTTACAGAGA ACGCAGAGGT TAAACTGGTG GATTTTGGGG TGAGCGCACA GTTGGATCGT 
ACTGTCGGCA GAAGAAACAC TTTCATTGGC ACTCCATACT GGATGGCCCC AGAAGTCATT 
GCCTGTGATG AAAATCCCGA CTCCACCTAT GATTACAGGA GTGATATCTG GTCTCTGGGA 
ATCACTGCTA TAGAAATGGC TGAAGGAGCA CCTCCGTTGT GTGACATGCA TCCCATGCGA 
GCTTTGTTCC TTATCCCACG GAACCCTCCT CCCAAACTGA AGTCCAAGAA ATGGTCTAAG 
AAGTTCATTG ACTTCATCGA GGGCTGTCTG GTAAAGTCAT ATCCGAATCG TCCGTCCACT 
GAACAGCTCC TGAAACACTC ATTCATCAGA GATCAGCCCA CTGAAAGACA AGTCCGCATC 
CAGCTCAAAG ATCACATCGA CAGAACACGC AAGAAACGTG GCGAAAAAGA GGAGACAGAG 
TACGAGTACA GCGGCAGTGA TGAAGAGGAT GAGAACAGAG GAGATGAGAG AGAATCCAGC 
TCTATTTTGA ATGTTCCTGG TGAGTCGACG TTGCGGAGGG ATTTCCTGCG GCTGCAGCAA 
GAAAATAAAG AGCGTTCAGA GGCCTTAAAG AGACAGCAGG CGCAGCTCGC CGCTCAGCGC 
AGAGACCCTG AGGAGCATAA ACGCCAGCTG CTGCACGACC GGCAGAAGCG CATAGAGGAG 
CAGAAAGAGC AGAGGCGACG CTTAGAGGAG CAACAGAGGA AGGAGAGAGA GATGGTGCGG 
CAACAACAGG ATAAAGCTCC TCACCGGAGA CTCGATGACA TGAGACGAGA GGAGGACAGG 
AGGTTGGCAG AGAGAGAGCA GGAGTATAAG CGTAAACAGC TGGAGGAGCA GAGACAGTCA 
GAGAGGCTTC AGAGGCAGTT ACAGCAGGAA CATGCGTATC TGGTCTCTCT ACAGCAACAG 
CAGCAGCAGC AAGACAAGAA ACCACAGATG TATCACTACA GCAAGAACCT AGAGAACAAC 
AAACCAGCCT GGGCTAGAGA GGTCGAGGAG CGCAGTAAGC TGAACAGACA GGGCTCTCCC 
AAAATCTGCA CCACGGTCTC TGACACTAAT ATTCAATCCC GCTCTGAGTC AATCAACCAA 
TCAGGAGCTG CGCAGACGCC GCCCATGCAG AGACCTGTGG AGCCGCAGGG TGGACAGGGA 
AAGATGGCCC ATCTAGTGCC GTTAAAACCA TACGCCGCTC CAGTCCCTCG CTCTCAGTCT 
CTTTGTGATC AGCCCACTAA GACCATGTCC GCCTTCCCCA CCCAGGACCC CTCACCCACA 
CCCACACCTC GTCCTCTACA CTCCAGAGAA CTAGTCCGTC AAAACTCTGA CCCCACCTCA 
GAGACTCCTG CCCCTCAGTC CCGGCCAATG AGAGACGACC GCGGACCCTG GATCCGTCTT 
CCTGAGATTG AACAACCTCC TAAAATTCCT CAGAGAACAG CCTCTATAGC CACTGCACTG 
AACACAAATC TCTCCTCTGG TATCAGACAC CCAGTCCGAG CAAGTAATCC AGATCTGAGT 
CGCAATGAGC GCTGGGAGAG AGGAGACAGC ATGAGCATCA TGTCCAACCT GCCACAGACC 
GGCTCTCTGG AGAGACACCG CATACTCAGC TCATCTAAGA TGGATTCCTC TCCAACCCTC 
TCTCATGATG GCCGACACAA ACCAGGAGAA TCCCGAACCT CATCTAGACC CAGCAGACCA 
GCGAGCTATA AGAGAGCAAT AGGAGAGGAT CATGGTCTGT ACTCTAAGGA GCGAGCAGAG 
GAGCAGCCGC GGCCCCCTGT GAAGGCCAAC GACTACTCCT CTTCCTCAGA GAGCAGTGAA 
AGCAGCGAAG AGAGTGAAAG TGGAGAAGGA GCAGAGGAGG AAAGCCCTAC TGACCGCCCC 
CGTGATGCAG ATTCTGACTC TGTGAACACT ATGGTTGTCC ATGAAGAGGA GGAAGGTGAA 
GGAGGAGAAG AAGAACGTGC TGGAGGATAT GGAGACCAAA CCATGCTTGT GCAGAGGACT 
CCAGAGAAGC GCAGTCATAA TGGATACACC AACCTGCCTG ATGTGGTTCA GCCTTCTCAC 
TCTCCCACAG ACTCGGCCTC CCACTCCTCC CCCGGGAAAG ACTCTACTTA TGATTATCAG 
TCCAGGGGAT TGGTCAAGGC CTCTGGAAAA TCCTCTTTTA CTACCTTTGT GGACCTTGGA 
ATGTACCAGC CATCAGGGGG TAGTGGAGAT AACATTTCTG TTGGAGGTCT GGGCTCTCGT 
TTTGAGCAGC TAAAGCTGGA GGTGAGGAAG GGCTCCATGG TCAATGTCAA TCCCACAAAC 
ACACGTCCGG TCAGTGACAC TCCTGAAATC CGCAAATACA AGAAGAGATT TAACTCTGAG 
ATCCTCTGCG CTGCTCTCTG GGGTGTGAAT CTGCTGGTCG GGACAGAGAA TGGTCTGAAG 
CTGCTGGACA GAAGCGGTCA GGGTAAAGTG TATCCACTGA TCAACTCGCG CAGATTTCAG 
CAGATGGATG TCCTGGAGGG ACTGAACCTC CTCATCACTA TATCAGGCAA GAAGAATAAG 
GTACGCGTGT ATTATCTGGC CTGGCTGAGG AATAAGATCC TCCATAATGA CCCAGAGGTG 
GAGAAGAAAC AGGGCTGGAC CACTGTAGGG GAAATGGAGG GCTGTGTGCA CTATAAAGTG 
GTGAAATATG AAAGGATTAA GTTTCTGGTT ATCGCTCTGA AGAATGCAGT GGAGGTTTAC 
GCCTGGGCTC CAAAACCTTA CCACAAGTTC ATGGCCTTCA AGTCATTTGG AGACCTGCCG 
CACAGACCTG TGTTGGTTGA CCTCACAGTG GAAGAGGGTC AGAGGTTAAA GGTCATCTAT 
GGCTCCTTGG CTGGCTTCCA TGCCATTGAT GTTGACTCTG GAAACAACTA CGACATCTAC 
ATTCCTGTAC ATATCCAGTC ACAGATCACT CCTCACGCCA TCGTGTTCCT GCCCAACTCA 
GATGGGATGG AGATGCTGTT GTGTTATGAG GACGAAGGTG TTTATGTCAA CACGTACGGA 
CGCATCATTA AAGATGTGGT GCTGCAGTGG GGAGAAATGC CCACATCAGT AGCCCACATT 
TGCTCTAATC AGATTATGGG TTGGGGAGAG AAAGCCATTG AGATCCGTTC TGTTGAGACG 
GGACACCTGG ATGGAGTCTT CATGCACAAG AGAGCCCAGA GGCTTAAGTT CCTGTGTGAA 
CGGAACGACA AGGTGTTTTT CGCGTCAGTG CGTTCCGGTG GCAGCAGCCA AGTTTACTTC 
ATGACCCTCA ACAGAAACTG CATTATGAAC TGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mink1 ( XM_017356009.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017211498.1 Danio rerio misshapen-like kinase 1 (mink1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	ODa59037
Clone ID Related Accession (Same CDS sequence)	XM_017356009.1
Accession Version	XM_017356009.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3801bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	misshapen-like kinase 1 isoform X5
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGAAA ACGCACCTAC GCGGAGTCTG GATGACATCG ACCTGGCCGC CCTGCGGGAC 
CCAGCAGGGA TCTTCGAGTT GGTGGAGGTC GTGGGCAACG GCACCTATGG ACAAGTTTAC 
AAGGGTCGTC ATGTTAAGAC AGGTCAGCTG GCAGCCATAA AAGTCATGGA TGTGACTGAG 
GAAGAAGAGG AGGAGATTAA AGCGGAGATT AACATGCTGA AGAAGTACAG TCACCATAGA 
AATATCGCCA CCTACTATGG AGCCTTTGTG AAGAAGAGTC CTCCTGGACA CGATGACCAG 
CTCTGGTTGG TGATGGAGTT TTGTGGTGCC GGCTCAGTAA CAGACTTGGT GAAGAACACA 
AAAGGCAACT CTTTAAAAGA AGACTGGATC GCCTATATCT GCAGAGAGAT CCTTAGGGGT 
CTGTCTCACC TCCATGCTCA TAAAGTCATC CACCGAGACA TCAAGGGACA GAATGTGCTG 
CTTACAGAGA ACGCAGAGGT TAAACTGGTG GATTTTGGGG TGAGCGCACA GTTGGATCGT 
ACTGTCGGCA GAAGAAACAC TTTCATTGGC ACTCCATACT GGATGGCCCC AGAAGTCATT 
GCCTGTGATG AAAATCCCGA CTCCACCTAT GATTACAGGA GTGATATCTG GTCTCTGGGA 
ATCACTGCTA TAGAAATGGC TGAAGGAGCA CCTCCGTTGT GTGACATGCA TCCCATGCGA 
GCTTTGTTCC TTATCCCACG GAACCCTCCT CCCAAACTGA AGTCCAAGAA ATGGTCTAAG 
AAGTTCATTG ACTTCATCGA GGGCTGTCTG GTAAAGTCAT ATCCGAATCG TCCGTCCACT 
GAACAGCTCC TGAAACACTC ATTCATCAGA GATCAGCCCA CTGAAAGACA AGTCCGCATC 
CAGCTCAAAG ATCACATCGA CAGAACACGC AAGAAACGTG GCGAAAAAGA GGAGACAGAG 
TACGAGTACA GCGGCAGTGA TGAAGAGGAT GAGAACAGAG GAGATGAGAG AGAATCCAGC 
TCTATTTTGA ATGTTCCTGG TGAGTCGACG TTGCGGAGGG ATTTCCTGCG GCTGCAGCAA 
GAAAATAAAG AGCGTTCAGA GGCCTTAAAG AGACAGCAGG CGCAGCTCGC CGCTCAGCGC 
AGAGACCCTG AGGAGCATAA ACGCCAGCTG CTGCACGACC GGCAGAAGCG CATAGAGGAG 
CAGAAAGAGC AGAGGCGACG CTTAGAGGAG CAACAGAGGA AGGAGAGAGA GATGGTGCGG 
CAACAACAGG ATAAAGCTCC TCACCGGAGA CTCGATGACA TGAGACGAGA GGAGGACAGG 
AGGTTGGCAG AGAGAGAGCA GGAGTTTATA AGACATAAGT TGGAGGAAGA GCAGCGGCAG 
TTAGAGATAC TGCAGCAGCA ATTACTGCAG GAGCAGGCAT TGCTAATGGA GTATAAGCGT 
AAACAGCTGG AGGAGCAGAG ACAGTCAGAG AGGCTTCAGA GGCAGTTACA GCAGGAACAT 
GCGTATCTGG TCTCTCTACA GCAACAGCAG CAGCAGCAAG ACAAGAAACC ACAGATGTAT 
CACTACAGCA AGAACCTAGA GAACAACAAA CCAGCCTGGG CTAGAGAGGT CGAGGAGCGC 
AGTAAGCTGA ACAGACAGGG CTCTCCCAAA ATCTGCACCA CGGTCTCTGA CACTAATATT 
CAATCCCGCT CTGAGTCAAT CAACCAATCA GGAGCTGCGC AGACGCCGCC CATGCAGAGA 
CCTGTGGAGC CGCAGGGTGG ACAGGGAAAG GACCCCTCAC CCACACCCAC ACCTCGTCCT 
CTACACTCCA GAGAACTAGT CCGTCAAAAC TCTGACCCCA CCTCAGAGAC TCCTGCCCCT 
CAGTCCCGGC CAATGAGAGA CGACCGCGGA CCCTGGATCC GTCTTCCTGA GATTGAACAA 
CCTCCTAAAA TTCCTCAGAG AACAGCCTCT ATAGCCACTG CACTGAACAC AAATCTCTCC 
TCTGGTATCA GACACCCAGT CCGAGCAAGT AATCCAGATC TGAGTCGCAA TGAGCGCTGG 
GAGAGAGGAG ACAGCATGAG CATCATGTCC AACCTGCCAC AGACCGGCTC TCTGGAGAGA 
CACCGCATAC TCAGCTCATC TAAGATGGAT TCCTCTCCAA CCCTCTCTCA TGATGGCCGA 
CACAAACCAG GAGAATCCCG AACCTCATCT AGACCCAGCA GACCAGCGAG CTATAAGAGA 
GCAATAGGAG AGGATCATGG TCTGTACTCT AAGGAGCGAG CAGAGGAGCA GCCGCGGCCC 
CCTGTGAAGG CCAACGACTA CTCCTCTTCC TCAGAGAGCA GTGAAAGCAG CGAAGAGAGT 
GAAAGTGGAG AAGGAGCAGA GGAGGAAAGC CCTACTGACC GCCCCCGTGA TGCAGATTCT 
GACTCTGTGA ACACTATGGT TGTCCATGAA GAGGAGGAAG GTGAAGGAGG AGAAGAAGAA 
CGTGCTGGAG GATATGGAGA CCAAACCATG CTTGTGCAGA GGACTCCAGA GAAGCGCAGT 
CATAATGGAT ACACCAACCT GCCTGATGTG GTTCAGCCTT CTCACTCTCC CACAGACTCG 
GCCTCCCACT CCTCCCCCGG GAAAGACTCT ACTTATGATT ATCAGTCCAG GGGATTGGTC 
AAGGCCTCTG GAAAATCCTC TTTTACTACC TTTGTGGACC TTGGAATGTA CCAGCCATCA 
GGGGGTAGTG GAGATAACAT TTCTGTTGGA GGTCTGGGCT CTCGTTTTGA GCAGCTAAAG 
CTGGAGGTGA GGAAGGGCTC CATGGTCAAT GTCAATCCCA CAAACACACG TCCGGTCAGT 
GACACTCCTG AAATCCGCAA ATACAAGAAG AGATTTAACT CTGAGATCCT CTGCGCTGCT 
CTCTGGGGTG TGAATCTGCT GGTCGGGACA GAGAATGGTC TGAAGCTGCT GGACAGAAGC 
GGTCAGGGTA AAGTGTATCC ACTGATCAAC TCGCGCAGAT TTCAGCAGAT GGATGTCCTG 
GAGGGACTGA ACCTCCTCAT CACTATATCA GGCAAGAAGA ATAAGGTACG CGTGTATTAT 
CTGGCCTGGC TGAGGAATAA GATCCTCCAT AATGACCCAG AGGTGGAGAA GAAACAGGGC 
TGGACCACTG TAGGGGAAAT GGAGGGCTGT GTGCACTATA AAGTGGTGAA ATATGAAAGG 
ATTAAGTTTC TGGTTATCGC TCTGAAGAAT GCAGTGGAGG TTTACGCCTG GGCTCCAAAA 
CCTTACCACA AGTTCATGGC CTTCAAGTCA TTTGGAGACC TGCCGCACAG ACCTGTGTTG 
GTTGACCTCA CAGTGGAAGA GGGTCAGAGG TTAAAGGTCA TCTATGGCTC CTTGGCTGGC 
TTCCATGCCA TTGATGTTGA CTCTGGAAAC AACTACGACA TCTACATTCC TGTACATATC 
CAGTCACAGA TCACTCCTCA CGCCATCGTG TTCCTGCCCA ACTCAGATGG GATGGAGATG 
CTGTTGTGTT ATGAGGACGA AGGTGTTTAT GTCAACACGT ACGGACGCAT CATTAAAGAT 
GTGGTGCTGC AGTGGGGAGA AATGCCCACA TCAGTAGCCC ACATTTGCTC TAATCAGATT 
ATGGGTTGGG GAGAGAAAGC CATTGAGATC CGTTCTGTTG AGACGGGACA CCTGGATGGA 
GTCTTCATGC ACAAGAGAGC CCAGAGGCTT AAGTTCCTGT GTGAACGGAA CGACAAGGTG 
TTTTTCGCGT CAGTGCGTTC CGGTGGCAGC AGCCAAGTTT ACTTCATGAC CCTCAACAGA 
AACTGCATTA TGAACTGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017211498.1
CDS	1243..5043
Translation	MSENAPTRSLDDIDLAALRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEEEEEEIKAEINMLKKYSHHRNIATYYGAFVKKSPPGHDDQLWLVMEFCGAGSVTDLVKNTKGNSLKEDWIAYICREILRGLSHLHAHKVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDSTYDYRSDIWSLGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPKLKSKKWSKKFIDFIEGCLVKSYPNRPSTEQLLKHSFIRDQPTERQVRIQLKDHIDRTRKKRGEKEETEYEYSGSDEEDENRGDERESSSILNVPGESTLRRDFLRLQQENKERSEALKRQQAQLAAQRRDPEEHKRQLLHDRQKRIEEQKEQRRRLEEQQRKEREMVRQQQDKAPHRRLDDMRREEDRRLAEREQEFIRHKLEEEQRQLEILQQQLLQEQALLMEYKRKQLEEQRQSERLQRQLQQEHAYLVSLQQQQQQQDKKPQMYHYSKNLENNKPAWAREVEERSKLNRQGSPKICTTVSDTNIQSRSESINQSGAAQTPPMQRPVEPQGGQGKDPSPTPTPRPLHSRELVRQNSDPTSETPAPQSRPMRDDRGPWIRLPEIEQPPKIPQRTASIATALNTNLSSGIRHPVRASNPDLSRNERWERGDSMSIMSNLPQTGSLERHRILSSSKMDSSPTLSHDGRHKPGESRTSSRPSRPASYKRAIGEDHGLYSKERAEEQPRPPVKANDYSSSSESSESSEESESGEGAEEESPTDRPRDADSDSVNTMVVHEEEEGEGGEEERAGGYGDQTMLVQRTPEKRSHNGYTNLPDVVQPSHSPTDSASHSSPGKDSTYDYQSRGLVKASGKSSFTTFVDLGMYQPSGGSGDNISVGGLGSRFEQLKLEVRKGSMVNVNPTNTRPVSDTPEIRKYKKRFNSEILCAALWGVNLLVGTENGLKLLDRSGQGKVYPLINSRRFQQMDVLEGLNLLITISGKKNKVRVYYLAWLRNKILHNDPEVEKKQGWTTVGEMEGCVHYKVVKYERIKFLVIALKNAVEVYAWAPKPYHKFMAFKSFGDLPHRPVLVDLTVEEGQRLKVIYGSLAGFHAIDVDSGNNYDIYIPVHIQSQITPHAIVFLPNSDGMEMLLCYEDEGVYVNTYGRIIKDVVLQWGEMPTSVAHICSNQIMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLNRNCIMNW

Target ORF information:

RefSeq Version	XM_017356009.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio misshapen-like kinase 1 (mink1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017356009.1

ORF Insert Sequence:

ATGTCAGAAA ACGCACCTAC GCGGAGTCTG GATGACATCG ACCTGGCCGC CCTGCGGGAC 
CCAGCAGGGA TCTTCGAGTT GGTGGAGGTC GTGGGCAACG GCACCTATGG ACAAGTTTAC 
AAGGGTCGTC ATGTTAAGAC AGGTCAGCTG GCAGCCATAA AAGTCATGGA TGTGACTGAG 
GAAGAAGAGG AGGAGATTAA AGCGGAGATT AACATGCTGA AGAAGTACAG TCACCATAGA 
AATATCGCCA CCTACTATGG AGCCTTTGTG AAGAAGAGTC CTCCTGGACA CGATGACCAG 
CTCTGGTTGG TGATGGAGTT TTGTGGTGCC GGCTCAGTAA CAGACTTGGT GAAGAACACA 
AAAGGCAACT CTTTAAAAGA AGACTGGATC GCCTATATCT GCAGAGAGAT CCTTAGGGGT 
CTGTCTCACC TCCATGCTCA TAAAGTCATC CACCGAGACA TCAAGGGACA GAATGTGCTG 
CTTACAGAGA ACGCAGAGGT TAAACTGGTG GATTTTGGGG TGAGCGCACA GTTGGATCGT 
ACTGTCGGCA GAAGAAACAC TTTCATTGGC ACTCCATACT GGATGGCCCC AGAAGTCATT 
GCCTGTGATG AAAATCCCGA CTCCACCTAT GATTACAGGA GTGATATCTG GTCTCTGGGA 
ATCACTGCTA TAGAAATGGC TGAAGGAGCA CCTCCGTTGT GTGACATGCA TCCCATGCGA 
GCTTTGTTCC TTATCCCACG GAACCCTCCT CCCAAACTGA AGTCCAAGAA ATGGTCTAAG 
AAGTTCATTG ACTTCATCGA GGGCTGTCTG GTAAAGTCAT ATCCGAATCG TCCGTCCACT 
GAACAGCTCC TGAAACACTC ATTCATCAGA GATCAGCCCA CTGAAAGACA AGTCCGCATC 
CAGCTCAAAG ATCACATCGA CAGAACACGC AAGAAACGTG GCGAAAAAGA GGAGACAGAG 
TACGAGTACA GCGGCAGTGA TGAAGAGGAT GAGAACAGAG GAGATGAGAG AGAATCCAGC 
TCTATTTTGA ATGTTCCTGG TGAGTCGACG TTGCGGAGGG ATTTCCTGCG GCTGCAGCAA 
GAAAATAAAG AGCGTTCAGA GGCCTTAAAG AGACAGCAGG CGCAGCTCGC CGCTCAGCGC 
AGAGACCCTG AGGAGCATAA ACGCCAGCTG CTGCACGACC GGCAGAAGCG CATAGAGGAG 
CAGAAAGAGC AGAGGCGACG CTTAGAGGAG CAACAGAGGA AGGAGAGAGA GATGGTGCGG 
CAACAACAGG ATAAAGCTCC TCACCGGAGA CTCGATGACA TGAGACGAGA GGAGGACAGG 
AGGTTGGCAG AGAGAGAGCA GGAGTTTATA AGACATAAGT TGGAGGAAGA GCAGCGGCAG 
TTAGAGATAC TGCAGCAGCA ATTACTGCAG GAGCAGGCAT TGCTAATGGA GTATAAGCGT 
AAACAGCTGG AGGAGCAGAG ACAGTCAGAG AGGCTTCAGA GGCAGTTACA GCAGGAACAT 
GCGTATCTGG TCTCTCTACA GCAACAGCAG CAGCAGCAAG ACAAGAAACC ACAGATGTAT 
CACTACAGCA AGAACCTAGA GAACAACAAA CCAGCCTGGG CTAGAGAGGT CGAGGAGCGC 
AGTAAGCTGA ACAGACAGGG CTCTCCCAAA ATCTGCACCA CGGTCTCTGA CACTAATATT 
CAATCCCGCT CTGAGTCAAT CAACCAATCA GGAGCTGCGC AGACGCCGCC CATGCAGAGA 
CCTGTGGAGC CGCAGGGTGG ACAGGGAAAG GACCCCTCAC CCACACCCAC ACCTCGTCCT 
CTACACTCCA GAGAACTAGT CCGTCAAAAC TCTGACCCCA CCTCAGAGAC TCCTGCCCCT 
CAGTCCCGGC CAATGAGAGA CGACCGCGGA CCCTGGATCC GTCTTCCTGA GATTGAACAA 
CCTCCTAAAA TTCCTCAGAG AACAGCCTCT ATAGCCACTG CACTGAACAC AAATCTCTCC 
TCTGGTATCA GACACCCAGT CCGAGCAAGT AATCCAGATC TGAGTCGCAA TGAGCGCTGG 
GAGAGAGGAG ACAGCATGAG CATCATGTCC AACCTGCCAC AGACCGGCTC TCTGGAGAGA 
CACCGCATAC TCAGCTCATC TAAGATGGAT TCCTCTCCAA CCCTCTCTCA TGATGGCCGA 
CACAAACCAG GAGAATCCCG AACCTCATCT AGACCCAGCA GACCAGCGAG CTATAAGAGA 
GCAATAGGAG AGGATCATGG TCTGTACTCT AAGGAGCGAG CAGAGGAGCA GCCGCGGCCC 
CCTGTGAAGG CCAACGACTA CTCCTCTTCC TCAGAGAGCA GTGAAAGCAG CGAAGAGAGT 
GAAAGTGGAG AAGGAGCAGA GGAGGAAAGC CCTACTGACC GCCCCCGTGA TGCAGATTCT 
GACTCTGTGA ACACTATGGT TGTCCATGAA GAGGAGGAAG GTGAAGGAGG AGAAGAAGAA 
CGTGCTGGAG GATATGGAGA CCAAACCATG CTTGTGCAGA GGACTCCAGA GAAGCGCAGT 
CATAATGGAT ACACCAACCT GCCTGATGTG GTTCAGCCTT CTCACTCTCC CACAGACTCG 
GCCTCCCACT CCTCCCCCGG GAAAGACTCT ACTTATGATT ATCAGTCCAG GGGATTGGTC 
AAGGCCTCTG GAAAATCCTC TTTTACTACC TTTGTGGACC TTGGAATGTA CCAGCCATCA 
GGGGGTAGTG GAGATAACAT TTCTGTTGGA GGTCTGGGCT CTCGTTTTGA GCAGCTAAAG 
CTGGAGGTGA GGAAGGGCTC CATGGTCAAT GTCAATCCCA CAAACACACG TCCGGTCAGT 
GACACTCCTG AAATCCGCAA ATACAAGAAG AGATTTAACT CTGAGATCCT CTGCGCTGCT 
CTCTGGGGTG TGAATCTGCT GGTCGGGACA GAGAATGGTC TGAAGCTGCT GGACAGAAGC 
GGTCAGGGTA AAGTGTATCC ACTGATCAAC TCGCGCAGAT TTCAGCAGAT GGATGTCCTG 
GAGGGACTGA ACCTCCTCAT CACTATATCA GGCAAGAAGA ATAAGGTACG CGTGTATTAT 
CTGGCCTGGC TGAGGAATAA GATCCTCCAT AATGACCCAG AGGTGGAGAA GAAACAGGGC 
TGGACCACTG TAGGGGAAAT GGAGGGCTGT GTGCACTATA AAGTGGTGAA ATATGAAAGG 
ATTAAGTTTC TGGTTATCGC TCTGAAGAAT GCAGTGGAGG TTTACGCCTG GGCTCCAAAA 
CCTTACCACA AGTTCATGGC CTTCAAGTCA TTTGGAGACC TGCCGCACAG ACCTGTGTTG 
GTTGACCTCA CAGTGGAAGA GGGTCAGAGG TTAAAGGTCA TCTATGGCTC CTTGGCTGGC 
TTCCATGCCA TTGATGTTGA CTCTGGAAAC AACTACGACA TCTACATTCC TGTACATATC 
CAGTCACAGA TCACTCCTCA CGCCATCGTG TTCCTGCCCA ACTCAGATGG GATGGAGATG 
CTGTTGTGTT ATGAGGACGA AGGTGTTTAT GTCAACACGT ACGGACGCAT CATTAAAGAT 
GTGGTGCTGC AGTGGGGAGA AATGCCCACA TCAGTAGCCC ACATTTGCTC TAATCAGATT 
ATGGGTTGGG GAGAGAAAGC CATTGAGATC CGTTCTGTTG AGACGGGACA CCTGGATGGA 
GTCTTCATGC ACAAGAGAGC CCAGAGGCTT AAGTTCCTGT GTGAACGGAA CGACAAGGTG 
TTTTTCGCGT CAGTGCGTTC CGGTGGCAGC AGCCAAGTTT ACTTCATGAC CCTCAACAGA 
AACTGCATTA TGAACTGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mink1 ( XM_017356010.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017211499.1 Danio rerio misshapen-like kinase 1 (mink1), transcript variant X6, mRNA.

Price & Availability↑

CloneID	ODa59038
Clone ID Related Accession (Same CDS sequence)	XM_017356010.1 , XM_017356010.2
Accession Version	XM_017356010.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3798bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	misshapen-like kinase 1 isoform X6
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGAAA ACGCACCTAC GCGGAGTCTG GATGACATCG ACCTGGCCGC CCTGCGGGAC 
CCAGCAGGGA TCTTCGAGTT GGTGGAGGTC GTGGGCAACG GCACCTATGG ACAAGTTTAC 
AAGGGTCGTC ATGTTAAGAC AGGTCAGCTG GCAGCCATAA AAGTCATGGA TGTGACTGAG 
GAAGAAGAGG AGGAGATTAA AGCGGAGATT AACATGCTGA AGAAGTACAG TCACCATAGA 
AATATCGCCA CCTACTATGG AGCCTTTGTG AAGAAGAGTC CTCCTGGACA CGATGACCAG 
CTCTGGTTGG TGATGGAGTT TTGTGGTGCC GGCTCAGTAA CAGACTTGGT GAAGAACACA 
AAAGGCAACT CTTTAAAAGA AGACTGGATC GCCTATATCT GCAGAGAGAT CCTTAGGGGT 
CTGTCTCACC TCCATGCTCA TAAAGTCATC CACCGAGACA TCAAGGGACA GAATGTGCTG 
CTTACAGAGA ACGCAGAGGT TAAACTGGTG GATTTTGGGG TGAGCGCACA GTTGGATCGT 
ACTGTCGGCA GAAGAAACAC TTTCATTGGC ACTCCATACT GGATGGCCCC AGAAGTCATT 
GCCTGTGATG AAAATCCCGA CTCCACCTAT GATTACAGGA GTGATATCTG GTCTCTGGGA 
ATCACTGCTA TAGAAATGGC TGAAGGAGCA CCTCCGTTGT GTGACATGCA TCCCATGCGA 
GCTTTGTTCC TTATCCCACG GAACCCTCCT CCCAAACTGA AGTCCAAGAA ATGGTCTAAG 
AAGTTCATTG ACTTCATCGA GGGCTGTCTG GTAAAGTCAT ATCCGAATCG TCCGTCCACT 
GAACAGCTCC TGAAACACTC ATTCATCAGA GATCAGCCCA CTGAAAGACA AGTCCGCATC 
CAGCTCAAAG ATCACATCGA CAGAACACGC AAGAAACGTG GCGAAAAAGA GGAGACAGAG 
TACGAGTACA GCGGCAGTGA TGAAGAGGAT GAGAACAGAG GAGATGAGAG AGAATCCAGC 
TCTATTTTGA ATGTTCCTGG TGAGTCGACG TTGCGGAGGG ATTTCCTGCG GCTGCAGCAA 
GAAAATAAAG AGCGTTCAGA GGCCTTAAAG AGACAGCAGG CGCAGCTCGC CGCTCAGCGC 
AGAGACCCTG AGGAGCATAA ACGCCAGCTG CTGCACGACC GGCAGAAGCG CATAGAGGAG 
CAGAAAGAGC AGAGGCGACG CTTAGAGGAG CAACAGAGGA AGGAGAGAGA GATGGTGCGG 
CAACAACAGG ATAAAGCTCC TCACCGGAGA CTCGATGACA TGAGACGAGA GGAGGACAGG 
AGGTTGGCAG AGAGAGAGCA GGAGTATAAG CGTAAACAGC TGGAGGAGCA GAGACAGTCA 
GAGAGGCTTC AGAGGCAGTT ACAGCAGGAA CATGCGTATC TGGTCTCTCT ACAGCAACAG 
CAGCAGCAGC AAGACAAGAA ACCACAGATG TATCACTACA GCAAGAACCT AGAGAACAAC 
AAACCAGCCT GGGCTAGAGA GGTCGAGGAG CGCAGTAAGC TGAACAGACA GGGCTCTCCC 
AAAATCTGCA CCACGGTCTC TGACACTAAT ATTCAATCCC GCTCTGAGTC AATCAACCAA 
TCAGGAGCTG CGCAGACGCC GCCCATGCAG AGACCTGTGG AGCCGCAGGG TGGACAGGGA 
AAGTTCCAGA TGGCCCATCT AGTGCCGTTA AAACCATACG CCGCTCCAGT CCCTCGCTCT 
CAGTCTCTTT GTGATCAGCC CACTAAGACC ATGTCCGCCT TCCCCACCCA GGACCCCTCA 
CCCACACCCA CACCTCGTCC TCTACACTCC AGAGAACTAG TCCGTCAAAA CTCTGACCCC 
ACCTCAGAGA CTCCTGCCCC TCAGTCCCGG CCAATGAGAG ACGACCGCGG ACCCTGGATC 
CGTCTTCCTG AGATTGAACA ACCTCCTAAA ATTCCTCAGA GAACAGCCTC TATAGCCACT 
GCACTGAACA CAAATCTCTC CTCTGGTATC AGACACCCAG TCCGAGCAAG TAATCCAGAT 
CTGAGTCGCA ATGAGCGCTG GGAGAGAGGA GACAGCATGA GCATCATGTC CAACCTGCCA 
CAGACCGGCT CTCTGGAGAG ACACCGCATA CTCAGCTCAT CTAAGATGGA TTCCTCTCCA 
ACCCTCTCTC ATGATGGCCG ACACAAACCA GGAGAATCCC GAACCTCATC TAGACCCAGC 
AGACCAGCGG ATCATGGTCT GTACTCTAAG GAGCGAGCAG AGGAGCAGCC GCGGCCCCCT 
GTGAAGGCCA ACGACTACTC CTCTTCCTCA GAGAGCAGTG AAAGCAGCGA AGAGAGTGAA 
AGTGGAGAAG GAGCAGAGGA GGAAAGCCCT ACTGACCGCC CCCGTGATGC AGATTCTGAC 
TCTGTGAACA CTATGGTTGT CCATGAAGAG GAGGAAGGTG AAGGAGGAGA AGAAGAACGT 
GCTGGAGGAT ATGGAGACCA AACCATGCTT GTGCAGAGGA CTCCAGAGAA GCGCAGTCAT 
AATGGATACA CCAACCTGCC TGATGTGGTT CAGCCTTCTC ACTCTCCCAC AGACTCGGCC 
TCCCACTCCT CCCCCGGGAA AGACTCTACT TATGATTATC AGTCCAGGGG ATTGGTCAAG 
GCCTCTGGAA AATCCTCTTT TACTACCTTT GTGGACCTTG GAATGTACCA GCCATCAGGG 
GGTAGTGGAG ATAACATTTC TGTTGGAGGT CTGGGCTCTC GTTTTGAGCA GCTAAAGCTG 
GAGGTGAGGA AGGGCTCCAT GGTCAATGTC AATCCCACAA ACACACGTCC GGTCAGTGAC 
ACTCCTGAAA TCCGCAAATA CAAGAAGAGA TTTAACTCTG AGATCCTCTG CGCTGCTCTC 
TGGGGTGTGA ATCTGCTGGT CGGGACAGAG AATGGTCTGA AGCTGCTGGA CAGAAGCGGT 
CAGGGTAAAG TGTATCCACT GATCAACTCG CGCAGATTTC AGCAGATGGA TGTCCTGGAG 
GGACTGAACC TCCTCATCAC TATATCAGGC AAGAAGAATA AGGTACGCGT GTATTATCTG 
GCCTGGCTGA GGAATAAGAT CCTCCATAAT GACCCAGAGG TGGAGAAGAA ACAGGGCTGG 
ACCACTGTAG GGGAAATGGA GGGCTGTGTG CACTATAAAG TGGTGAAATA TGAAAGGATT 
AAGTTTCTGG TTATCGCTCT GAAGAATGCA GTGGAGGTTT ACGCCTGGGC TCCAAAACCT 
TACCACAAGT TCATGGCCTT CAAGTCATTT GGAGACCTGC CGCACAGACC TGTGTTGGTT 
GACCTCACAG TGGAAGAGGG TCAGAGGTTA AAGGTCATCT ATGGCTCCTT GGCTGGCTTC 
CATGCCATTG ATGTTGACTC TGGAAACAAC TACGACATCT ACATTCCTGT ACATATCCAG 
TCACAGATCA CTCCTCACGC CATCGTGTTC CTGCCCAACT CAGATGGGAT GGAGATGCTG 
TTGTGTTATG AGGACGAAGG TGTTTATGTC AACACGTACG GACGCATCAT TAAAGATGTG 
GTGCTGCAGT GGGGAGAAAT GCCCACATCA GTAGCCCACA TTTGCTCTAA TCAGATTATG 
GGTTGGGGAG AGAAAGCCAT TGAGATCCGT TCTGTTGAGA CGGGACACCT GGATGGAGTC 
TTCATGCACA AGAGAGCCCA GAGGCTTAAG TTCCTGTGTG AACGGAACGA CAAGGTGTTT 
TTCGCGTCAG TGCGTTCCGG TGGCAGCAGC CAAGTTTACT TCATGACCCT CAACAGAAAC 
TGCATTATGA ACTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017211499.1
CDS	1244..5041
Translation	MSENAPTRSLDDIDLAALRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEEEEEEIKAEINMLKKYSHHRNIATYYGAFVKKSPPGHDDQLWLVMEFCGAGSVTDLVKNTKGNSLKEDWIAYICREILRGLSHLHAHKVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDSTYDYRSDIWSLGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPKLKSKKWSKKFIDFIEGCLVKSYPNRPSTEQLLKHSFIRDQPTERQVRIQLKDHIDRTRKKRGEKEETEYEYSGSDEEDENRGDERESSSILNVPGESTLRRDFLRLQQENKERSEALKRQQAQLAAQRRDPEEHKRQLLHDRQKRIEEQKEQRRRLEEQQRKEREMVRQQQDKAPHRRLDDMRREEDRRLAEREQEYKRKQLEEQRQSERLQRQLQQEHAYLVSLQQQQQQQDKKPQMYHYSKNLENNKPAWAREVEERSKLNRQGSPKICTTVSDTNIQSRSESINQSGAAQTPPMQRPVEPQGGQGKFQMAHLVPLKPYAAPVPRSQSLCDQPTKTMSAFPTQDPSPTPTPRPLHSRELVRQNSDPTSETPAPQSRPMRDDRGPWIRLPEIEQPPKIPQRTASIATALNTNLSSGIRHPVRASNPDLSRNERWERGDSMSIMSNLPQTGSLERHRILSSSKMDSSPTLSHDGRHKPGESRTSSRPSRPADHGLYSKERAEEQPRPPVKANDYSSSSESSESSEESESGEGAEEESPTDRPRDADSDSVNTMVVHEEEEGEGGEEERAGGYGDQTMLVQRTPEKRSHNGYTNLPDVVQPSHSPTDSASHSSPGKDSTYDYQSRGLVKASGKSSFTTFVDLGMYQPSGGSGDNISVGGLGSRFEQLKLEVRKGSMVNVNPTNTRPVSDTPEIRKYKKRFNSEILCAALWGVNLLVGTENGLKLLDRSGQGKVYPLINSRRFQQMDVLEGLNLLITISGKKNKVRVYYLAWLRNKILHNDPEVEKKQGWTTVGEMEGCVHYKVVKYERIKFLVIALKNAVEVYAWAPKPYHKFMAFKSFGDLPHRPVLVDLTVEEGQRLKVIYGSLAGFHAIDVDSGNNYDIYIPVHIQSQITPHAIVFLPNSDGMEMLLCYEDEGVYVNTYGRIIKDVVLQWGEMPTSVAHICSNQIMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLNRNCIMNW

Target ORF information:

RefSeq Version	XM_017356010.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio misshapen-like kinase 1 (mink1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017356010.1

ORF Insert Sequence:

ATGTCAGAAA ACGCACCTAC GCGGAGTCTG GATGACATCG ACCTGGCCGC CCTGCGGGAC 
CCAGCAGGGA TCTTCGAGTT GGTGGAGGTC GTGGGCAACG GCACCTATGG ACAAGTTTAC 
AAGGGTCGTC ATGTTAAGAC AGGTCAGCTG GCAGCCATAA AAGTCATGGA TGTGACTGAG 
GAAGAAGAGG AGGAGATTAA AGCGGAGATT AACATGCTGA AGAAGTACAG TCACCATAGA 
AATATCGCCA CCTACTATGG AGCCTTTGTG AAGAAGAGTC CTCCTGGACA CGATGACCAG 
CTCTGGTTGG TGATGGAGTT TTGTGGTGCC GGCTCAGTAA CAGACTTGGT GAAGAACACA 
AAAGGCAACT CTTTAAAAGA AGACTGGATC GCCTATATCT GCAGAGAGAT CCTTAGGGGT 
CTGTCTCACC TCCATGCTCA TAAAGTCATC CACCGAGACA TCAAGGGACA GAATGTGCTG 
CTTACAGAGA ACGCAGAGGT TAAACTGGTG GATTTTGGGG TGAGCGCACA GTTGGATCGT 
ACTGTCGGCA GAAGAAACAC TTTCATTGGC ACTCCATACT GGATGGCCCC AGAAGTCATT 
GCCTGTGATG AAAATCCCGA CTCCACCTAT GATTACAGGA GTGATATCTG GTCTCTGGGA 
ATCACTGCTA TAGAAATGGC TGAAGGAGCA CCTCCGTTGT GTGACATGCA TCCCATGCGA 
GCTTTGTTCC TTATCCCACG GAACCCTCCT CCCAAACTGA AGTCCAAGAA ATGGTCTAAG 
AAGTTCATTG ACTTCATCGA GGGCTGTCTG GTAAAGTCAT ATCCGAATCG TCCGTCCACT 
GAACAGCTCC TGAAACACTC ATTCATCAGA GATCAGCCCA CTGAAAGACA AGTCCGCATC 
CAGCTCAAAG ATCACATCGA CAGAACACGC AAGAAACGTG GCGAAAAAGA GGAGACAGAG 
TACGAGTACA GCGGCAGTGA TGAAGAGGAT GAGAACAGAG GAGATGAGAG AGAATCCAGC 
TCTATTTTGA ATGTTCCTGG TGAGTCGACG TTGCGGAGGG ATTTCCTGCG GCTGCAGCAA 
GAAAATAAAG AGCGTTCAGA GGCCTTAAAG AGACAGCAGG CGCAGCTCGC CGCTCAGCGC 
AGAGACCCTG AGGAGCATAA ACGCCAGCTG CTGCACGACC GGCAGAAGCG CATAGAGGAG 
CAGAAAGAGC AGAGGCGACG CTTAGAGGAG CAACAGAGGA AGGAGAGAGA GATGGTGCGG 
CAACAACAGG ATAAAGCTCC TCACCGGAGA CTCGATGACA TGAGACGAGA GGAGGACAGG 
AGGTTGGCAG AGAGAGAGCA GGAGTATAAG CGTAAACAGC TGGAGGAGCA GAGACAGTCA 
GAGAGGCTTC AGAGGCAGTT ACAGCAGGAA CATGCGTATC TGGTCTCTCT ACAGCAACAG 
CAGCAGCAGC AAGACAAGAA ACCACAGATG TATCACTACA GCAAGAACCT AGAGAACAAC 
AAACCAGCCT GGGCTAGAGA GGTCGAGGAG CGCAGTAAGC TGAACAGACA GGGCTCTCCC 
AAAATCTGCA CCACGGTCTC TGACACTAAT ATTCAATCCC GCTCTGAGTC AATCAACCAA 
TCAGGAGCTG CGCAGACGCC GCCCATGCAG AGACCTGTGG AGCCGCAGGG TGGACAGGGA 
AAGTTCCAGA TGGCCCATCT AGTGCCGTTA AAACCATACG CCGCTCCAGT CCCTCGCTCT 
CAGTCTCTTT GTGATCAGCC CACTAAGACC ATGTCCGCCT TCCCCACCCA GGACCCCTCA 
CCCACACCCA CACCTCGTCC TCTACACTCC AGAGAACTAG TCCGTCAAAA CTCTGACCCC 
ACCTCAGAGA CTCCTGCCCC TCAGTCCCGG CCAATGAGAG ACGACCGCGG ACCCTGGATC 
CGTCTTCCTG AGATTGAACA ACCTCCTAAA ATTCCTCAGA GAACAGCCTC TATAGCCACT 
GCACTGAACA CAAATCTCTC CTCTGGTATC AGACACCCAG TCCGAGCAAG TAATCCAGAT 
CTGAGTCGCA ATGAGCGCTG GGAGAGAGGA GACAGCATGA GCATCATGTC CAACCTGCCA 
CAGACCGGCT CTCTGGAGAG ACACCGCATA CTCAGCTCAT CTAAGATGGA TTCCTCTCCA 
ACCCTCTCTC ATGATGGCCG ACACAAACCA GGAGAATCCC GAACCTCATC TAGACCCAGC 
AGACCAGCGG ATCATGGTCT GTACTCTAAG GAGCGAGCAG AGGAGCAGCC GCGGCCCCCT 
GTGAAGGCCA ACGACTACTC CTCTTCCTCA GAGAGCAGTG AAAGCAGCGA AGAGAGTGAA 
AGTGGAGAAG GAGCAGAGGA GGAAAGCCCT ACTGACCGCC CCCGTGATGC AGATTCTGAC 
TCTGTGAACA CTATGGTTGT CCATGAAGAG GAGGAAGGTG AAGGAGGAGA AGAAGAACGT 
GCTGGAGGAT ATGGAGACCA AACCATGCTT GTGCAGAGGA CTCCAGAGAA GCGCAGTCAT 
AATGGATACA CCAACCTGCC TGATGTGGTT CAGCCTTCTC ACTCTCCCAC AGACTCGGCC 
TCCCACTCCT CCCCCGGGAA AGACTCTACT TATGATTATC AGTCCAGGGG ATTGGTCAAG 
GCCTCTGGAA AATCCTCTTT TACTACCTTT GTGGACCTTG GAATGTACCA GCCATCAGGG 
GGTAGTGGAG ATAACATTTC TGTTGGAGGT CTGGGCTCTC GTTTTGAGCA GCTAAAGCTG 
GAGGTGAGGA AGGGCTCCAT GGTCAATGTC AATCCCACAA ACACACGTCC GGTCAGTGAC 
ACTCCTGAAA TCCGCAAATA CAAGAAGAGA TTTAACTCTG AGATCCTCTG CGCTGCTCTC 
TGGGGTGTGA ATCTGCTGGT CGGGACAGAG AATGGTCTGA AGCTGCTGGA CAGAAGCGGT 
CAGGGTAAAG TGTATCCACT GATCAACTCG CGCAGATTTC AGCAGATGGA TGTCCTGGAG 
GGACTGAACC TCCTCATCAC TATATCAGGC AAGAAGAATA AGGTACGCGT GTATTATCTG 
GCCTGGCTGA GGAATAAGAT CCTCCATAAT GACCCAGAGG TGGAGAAGAA ACAGGGCTGG 
ACCACTGTAG GGGAAATGGA GGGCTGTGTG CACTATAAAG TGGTGAAATA TGAAAGGATT 
AAGTTTCTGG TTATCGCTCT GAAGAATGCA GTGGAGGTTT ACGCCTGGGC TCCAAAACCT 
TACCACAAGT TCATGGCCTT CAAGTCATTT GGAGACCTGC CGCACAGACC TGTGTTGGTT 
GACCTCACAG TGGAAGAGGG TCAGAGGTTA AAGGTCATCT ATGGCTCCTT GGCTGGCTTC 
CATGCCATTG ATGTTGACTC TGGAAACAAC TACGACATCT ACATTCCTGT ACATATCCAG 
TCACAGATCA CTCCTCACGC CATCGTGTTC CTGCCCAACT CAGATGGGAT GGAGATGCTG 
TTGTGTTATG AGGACGAAGG TGTTTATGTC AACACGTACG GACGCATCAT TAAAGATGTG 
GTGCTGCAGT GGGGAGAAAT GCCCACATCA GTAGCCCACA TTTGCTCTAA TCAGATTATG 
GGTTGGGGAG AGAAAGCCAT TGAGATCCGT TCTGTTGAGA CGGGACACCT GGATGGAGTC 
TTCATGCACA AGAGAGCCCA GAGGCTTAAG TTCCTGTGTG AACGGAACGA CAAGGTGTTT 
TTCGCGTCAG TGCGTTCCGG TGGCAGCAGC CAAGTTTACT TCATGACCCT CAACAGAAAC 
TGCATTATGA ACTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mink1 ( XM_017356010.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017211499.1 Danio rerio misshapen-like kinase 1 (mink1), transcript variant X8, mRNA.

Price & Availability↑

CloneID	ODa59038
Clone ID Related Accession (Same CDS sequence)	XM_017356010.1 , XM_017356010.2
Accession Version	XM_017356010.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3798bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	misshapen-like kinase 1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017356010.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGAAA ACGCACCTAC GCGGAGTCTG GATGACATCG ACCTGGCCGC CCTGCGGGAC 
CCAGCAGGGA TCTTCGAGTT GGTGGAGGTC GTGGGCAACG GCACCTATGG ACAAGTTTAC 
AAGGGTCGTC ATGTTAAGAC AGGTCAGCTG GCAGCCATAA AAGTCATGGA TGTGACTGAG 
GAAGAAGAGG AGGAGATTAA AGCGGAGATT AACATGCTGA AGAAGTACAG TCACCATAGA 
AATATCGCCA CCTACTATGG AGCCTTTGTG AAGAAGAGTC CTCCTGGACA CGATGACCAG 
CTCTGGTTGG TGATGGAGTT TTGTGGTGCC GGCTCAGTAA CAGACTTGGT GAAGAACACA 
AAAGGCAACT CTTTAAAAGA AGACTGGATC GCCTATATCT GCAGAGAGAT CCTTAGGGGT 
CTGTCTCACC TCCATGCTCA TAAAGTCATC CACCGAGACA TCAAGGGACA GAATGTGCTG 
CTTACAGAGA ACGCAGAGGT TAAACTGGTG GATTTTGGGG TGAGCGCACA GTTGGATCGT 
ACTGTCGGCA GAAGAAACAC TTTCATTGGC ACTCCATACT GGATGGCCCC AGAAGTCATT 
GCCTGTGATG AAAATCCCGA CTCCACCTAT GATTACAGGA GTGATATCTG GTCTCTGGGA 
ATCACTGCTA TAGAAATGGC TGAAGGAGCA CCTCCGTTGT GTGACATGCA TCCCATGCGA 
GCTTTGTTCC TTATCCCACG GAACCCTCCT CCCAAACTGA AGTCCAAGAA ATGGTCTAAG 
AAGTTCATTG ACTTCATCGA GGGCTGTCTG GTAAAGTCAT ATCCGAATCG TCCGTCCACT 
GAACAGCTCC TGAAACACTC ATTCATCAGA GATCAGCCCA CTGAAAGACA AGTCCGCATC 
CAGCTCAAAG ATCACATCGA CAGAACACGC AAGAAACGTG GCGAAAAAGA GGAGACAGAG 
TACGAGTACA GCGGCAGTGA TGAAGAGGAT GAGAACAGAG GAGATGAGAG AGAATCCAGC 
TCTATTTTGA ATGTTCCTGG TGAGTCGACG TTGCGGAGGG ATTTCCTGCG GCTGCAGCAA 
GAAAATAAAG AGCGTTCAGA GGCCTTAAAG AGACAGCAGG CGCAGCTCGC CGCTCAGCGC 
AGAGACCCTG AGGAGCATAA ACGCCAGCTG CTGCACGACC GGCAGAAGCG CATAGAGGAG 
CAGAAAGAGC AGAGGCGACG CTTAGAGGAG CAACAGAGGA AGGAGAGAGA GATGGTGCGG 
CAACAACAGG ATAAAGCTCC TCACCGGAGA CTCGATGACA TGAGACGAGA GGAGGACAGG 
AGGTTGGCAG AGAGAGAGCA GGAGTATAAG CGTAAACAGC TGGAGGAGCA GAGACAGTCA 
GAGAGGCTTC AGAGGCAGTT ACAGCAGGAA CATGCGTATC TGGTCTCTCT ACAGCAACAG 
CAGCAGCAGC AAGACAAGAA ACCACAGATG TATCACTACA GCAAGAACCT AGAGAACAAC 
AAACCAGCCT GGGCTAGAGA GGTCGAGGAG CGCAGTAAGC TGAACAGACA GGGCTCTCCC 
AAAATCTGCA CCACGGTCTC TGACACTAAT ATTCAATCCC GCTCTGAGTC AATCAACCAA 
TCAGGAGCTG CGCAGACGCC GCCCATGCAG AGACCTGTGG AGCCGCAGGG TGGACAGGGA 
AAGTTCCAGA TGGCCCATCT AGTGCCGTTA AAACCATACG CCGCTCCAGT CCCTCGCTCT 
CAGTCTCTTT GTGATCAGCC CACTAAGACC ATGTCCGCCT TCCCCACCCA GGACCCCTCA 
CCCACACCCA CACCTCGTCC TCTACACTCC AGAGAACTAG TCCGTCAAAA CTCTGACCCC 
ACCTCAGAGA CTCCTGCCCC TCAGTCCCGG CCAATGAGAG ACGACCGCGG ACCCTGGATC 
CGTCTTCCTG AGATTGAACA ACCTCCTAAA ATTCCTCAGA GAACAGCCTC TATAGCCACT 
GCACTGAACA CAAATCTCTC CTCTGGTATC AGACACCCAG TCCGAGCAAG TAATCCAGAT 
CTGAGTCGCA ATGAGCGCTG GGAGAGAGGA GACAGCATGA GCATCATGTC CAACCTGCCA 
CAGACCGGCT CTCTGGAGAG ACACCGCATA CTCAGCTCAT CTAAGATGGA TTCCTCTCCA 
ACCCTCTCTC ATGATGGCCG ACACAAACCA GGAGAATCCC GAACCTCATC TAGACCCAGC 
AGACCAGCGG ATCATGGTCT GTACTCTAAG GAGCGAGCAG AGGAGCAGCC GCGGCCCCCT 
GTGAAGGCCA ACGACTACTC CTCTTCCTCA GAGAGCAGTG AAAGCAGCGA AGAGAGTGAA 
AGTGGAGAAG GAGCAGAGGA GGAAAGCCCT ACTGACCGCC CCCGTGATGC AGATTCTGAC 
TCTGTGAACA CTATGGTTGT CCATGAAGAG GAGGAAGGTG AAGGAGGAGA AGAAGAACGT 
GCTGGAGGAT ATGGAGACCA AACCATGCTT GTGCAGAGGA CTCCAGAGAA GCGCAGTCAT 
AATGGATACA CCAACCTGCC TGATGTGGTT CAGCCTTCTC ACTCTCCCAC AGACTCGGCC 
TCCCACTCCT CCCCCGGGAA AGACTCTACT TATGATTATC AGTCCAGGGG ATTGGTCAAG 
GCCTCTGGAA AATCCTCTTT TACTACCTTT GTGGACCTTG GAATGTACCA GCCATCAGGG 
GGTAGTGGAG ATAACATTTC TGTTGGAGGT CTGGGCTCTC GTTTTGAGCA GCTAAAGCTG 
GAGGTGAGGA AGGGCTCCAT GGTCAATGTC AATCCCACAA ACACACGTCC GGTCAGTGAC 
ACTCCTGAAA TCCGCAAATA CAAGAAGAGA TTTAACTCTG AGATCCTCTG CGCTGCTCTC 
TGGGGTGTGA ATCTGCTGGT CGGGACAGAG AATGGTCTGA AGCTGCTGGA CAGAAGCGGT 
CAGGGTAAAG TGTATCCACT GATCAACTCG CGCAGATTTC AGCAGATGGA TGTCCTGGAG 
GGACTGAACC TCCTCATCAC TATATCAGGC AAGAAGAATA AGGTACGCGT GTATTATCTG 
GCCTGGCTGA GGAATAAGAT CCTCCATAAT GACCCAGAGG TGGAGAAGAA ACAGGGCTGG 
ACCACTGTAG GGGAAATGGA GGGCTGTGTG CACTATAAAG TGGTGAAATA TGAAAGGATT 
AAGTTTCTGG TTATCGCTCT GAAGAATGCA GTGGAGGTTT ACGCCTGGGC TCCAAAACCT 
TACCACAAGT TCATGGCCTT CAAGTCATTT GGAGACCTGC CGCACAGACC TGTGTTGGTT 
GACCTCACAG TGGAAGAGGG TCAGAGGTTA AAGGTCATCT ATGGCTCCTT GGCTGGCTTC 
CATGCCATTG ATGTTGACTC TGGAAACAAC TACGACATCT ACATTCCTGT ACATATCCAG 
TCACAGATCA CTCCTCACGC CATCGTGTTC CTGCCCAACT CAGATGGGAT GGAGATGCTG 
TTGTGTTATG AGGACGAAGG TGTTTATGTC AACACGTACG GACGCATCAT TAAAGATGTG 
GTGCTGCAGT GGGGAGAAAT GCCCACATCA GTAGCCCACA TTTGCTCTAA TCAGATTATG 
GGTTGGGGAG AGAAAGCCAT TGAGATCCGT TCTGTTGAGA CGGGACACCT GGATGGAGTC 
TTCATGCACA AGAGAGCCCA GAGGCTTAAG TTCCTGTGTG AACGGAACGA CAAGGTGTTT 
TTCGCGTCAG TGCGTTCCGG TGGCAGCAGC CAAGTTTACT TCATGACCCT CAACAGAAAC 
TGCATTATGA ACTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017211499.1
CDS	1260..5057
Translation	MSENAPTRSLDDIDLAALRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEEEEEEIKAEINMLKKYSHHRNIATYYGAFVKKSPPGHDDQLWLVMEFCGAGSVTDLVKNTKGNSLKEDWIAYICREILRGLSHLHAHKVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDSTYDYRSDIWSLGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPKLKSKKWSKKFIDFIEGCLVKSYPNRPSTEQLLKHSFIRDQPTERQVRIQLKDHIDRTRKKRGEKEETEYEYSGSDEEDENRGDERESSSILNVPGESTLRRDFLRLQQENKERSEALKRQQAQLAAQRRDPEEHKRQLLHDRQKRIEEQKEQRRRLEEQQRKEREMVRQQQDKAPHRRLDDMRREEDRRLAEREQEYKRKQLEEQRQSERLQRQLQQEHAYLVSLQQQQQQQDKKPQMYHYSKNLENNKPAWAREVEERSKLNRQGSPKICTTVSDTNIQSRSESINQSGAAQTPPMQRPVEPQGGQGKFQMAHLVPLKPYAAPVPRSQSLCDQPTKTMSAFPTQDPSPTPTPRPLHSRELVRQNSDPTSETPAPQSRPMRDDRGPWIRLPEIEQPPKIPQRTASIATALNTNLSSGIRHPVRASNPDLSRNERWERGDSMSIMSNLPQTGSLERHRILSSSKMDSSPTLSHDGRHKPGESRTSSRPSRPADHGLYSKERAEEQPRPPVKANDYSSSSESSESSEESESGEGAEEESPTDRPRDADSDSVNTMVVHEEEEGEGGEEERAGGYGDQTMLVQRTPEKRSHNGYTNLPDVVQPSHSPTDSASHSSPGKDSTYDYQSRGLVKASGKSSFTTFVDLGMYQPSGGSGDNISVGGLGSRFEQLKLEVRKGSMVNVNPTNTRPVSDTPEIRKYKKRFNSEILCAALWGVNLLVGTENGLKLLDRSGQGKVYPLINSRRFQQMDVLEGLNLLITISGKKNKVRVYYLAWLRNKILHNDPEVEKKQGWTTVGEMEGCVHYKVVKYERIKFLVIALKNAVEVYAWAPKPYHKFMAFKSFGDLPHRPVLVDLTVEEGQRLKVIYGSLAGFHAIDVDSGNNYDIYIPVHIQSQITPHAIVFLPNSDGMEMLLCYEDEGVYVNTYGRIIKDVVLQWGEMPTSVAHICSNQIMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLNRNCIMNW

Target ORF information:

RefSeq Version	XM_017356010.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio misshapen-like kinase 1 (mink1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017356010.2

ORF Insert Sequence:

ATGTCAGAAA ACGCACCTAC GCGGAGTCTG GATGACATCG ACCTGGCCGC CCTGCGGGAC 
CCAGCAGGGA TCTTCGAGTT GGTGGAGGTC GTGGGCAACG GCACCTATGG ACAAGTTTAC 
AAGGGTCGTC ATGTTAAGAC AGGTCAGCTG GCAGCCATAA AAGTCATGGA TGTGACTGAG 
GAAGAAGAGG AGGAGATTAA AGCGGAGATT AACATGCTGA AGAAGTACAG TCACCATAGA 
AATATCGCCA CCTACTATGG AGCCTTTGTG AAGAAGAGTC CTCCTGGACA CGATGACCAG 
CTCTGGTTGG TGATGGAGTT TTGTGGTGCC GGCTCAGTAA CAGACTTGGT GAAGAACACA 
AAAGGCAACT CTTTAAAAGA AGACTGGATC GCCTATATCT GCAGAGAGAT CCTTAGGGGT 
CTGTCTCACC TCCATGCTCA TAAAGTCATC CACCGAGACA TCAAGGGACA GAATGTGCTG 
CTTACAGAGA ACGCAGAGGT TAAACTGGTG GATTTTGGGG TGAGCGCACA GTTGGATCGT 
ACTGTCGGCA GAAGAAACAC TTTCATTGGC ACTCCATACT GGATGGCCCC AGAAGTCATT 
GCCTGTGATG AAAATCCCGA CTCCACCTAT GATTACAGGA GTGATATCTG GTCTCTGGGA 
ATCACTGCTA TAGAAATGGC TGAAGGAGCA CCTCCGTTGT GTGACATGCA TCCCATGCGA 
GCTTTGTTCC TTATCCCACG GAACCCTCCT CCCAAACTGA AGTCCAAGAA ATGGTCTAAG 
AAGTTCATTG ACTTCATCGA GGGCTGTCTG GTAAAGTCAT ATCCGAATCG TCCGTCCACT 
GAACAGCTCC TGAAACACTC ATTCATCAGA GATCAGCCCA CTGAAAGACA AGTCCGCATC 
CAGCTCAAAG ATCACATCGA CAGAACACGC AAGAAACGTG GCGAAAAAGA GGAGACAGAG 
TACGAGTACA GCGGCAGTGA TGAAGAGGAT GAGAACAGAG GAGATGAGAG AGAATCCAGC 
TCTATTTTGA ATGTTCCTGG TGAGTCGACG TTGCGGAGGG ATTTCCTGCG GCTGCAGCAA 
GAAAATAAAG AGCGTTCAGA GGCCTTAAAG AGACAGCAGG CGCAGCTCGC CGCTCAGCGC 
AGAGACCCTG AGGAGCATAA ACGCCAGCTG CTGCACGACC GGCAGAAGCG CATAGAGGAG 
CAGAAAGAGC AGAGGCGACG CTTAGAGGAG CAACAGAGGA AGGAGAGAGA GATGGTGCGG 
CAACAACAGG ATAAAGCTCC TCACCGGAGA CTCGATGACA TGAGACGAGA GGAGGACAGG 
AGGTTGGCAG AGAGAGAGCA GGAGTATAAG CGTAAACAGC TGGAGGAGCA GAGACAGTCA 
GAGAGGCTTC AGAGGCAGTT ACAGCAGGAA CATGCGTATC TGGTCTCTCT ACAGCAACAG 
CAGCAGCAGC AAGACAAGAA ACCACAGATG TATCACTACA GCAAGAACCT AGAGAACAAC 
AAACCAGCCT GGGCTAGAGA GGTCGAGGAG CGCAGTAAGC TGAACAGACA GGGCTCTCCC 
AAAATCTGCA CCACGGTCTC TGACACTAAT ATTCAATCCC GCTCTGAGTC AATCAACCAA 
TCAGGAGCTG CGCAGACGCC GCCCATGCAG AGACCTGTGG AGCCGCAGGG TGGACAGGGA 
AAGTTCCAGA TGGCCCATCT AGTGCCGTTA AAACCATACG CCGCTCCAGT CCCTCGCTCT 
CAGTCTCTTT GTGATCAGCC CACTAAGACC ATGTCCGCCT TCCCCACCCA GGACCCCTCA 
CCCACACCCA CACCTCGTCC TCTACACTCC AGAGAACTAG TCCGTCAAAA CTCTGACCCC 
ACCTCAGAGA CTCCTGCCCC TCAGTCCCGG CCAATGAGAG ACGACCGCGG ACCCTGGATC 
CGTCTTCCTG AGATTGAACA ACCTCCTAAA ATTCCTCAGA GAACAGCCTC TATAGCCACT 
GCACTGAACA CAAATCTCTC CTCTGGTATC AGACACCCAG TCCGAGCAAG TAATCCAGAT 
CTGAGTCGCA ATGAGCGCTG GGAGAGAGGA GACAGCATGA GCATCATGTC CAACCTGCCA 
CAGACCGGCT CTCTGGAGAG ACACCGCATA CTCAGCTCAT CTAAGATGGA TTCCTCTCCA 
ACCCTCTCTC ATGATGGCCG ACACAAACCA GGAGAATCCC GAACCTCATC TAGACCCAGC 
AGACCAGCGG ATCATGGTCT GTACTCTAAG GAGCGAGCAG AGGAGCAGCC GCGGCCCCCT 
GTGAAGGCCA ACGACTACTC CTCTTCCTCA GAGAGCAGTG AAAGCAGCGA AGAGAGTGAA 
AGTGGAGAAG GAGCAGAGGA GGAAAGCCCT ACTGACCGCC CCCGTGATGC AGATTCTGAC 
TCTGTGAACA CTATGGTTGT CCATGAAGAG GAGGAAGGTG AAGGAGGAGA AGAAGAACGT 
GCTGGAGGAT ATGGAGACCA AACCATGCTT GTGCAGAGGA CTCCAGAGAA GCGCAGTCAT 
AATGGATACA CCAACCTGCC TGATGTGGTT CAGCCTTCTC ACTCTCCCAC AGACTCGGCC 
TCCCACTCCT CCCCCGGGAA AGACTCTACT TATGATTATC AGTCCAGGGG ATTGGTCAAG 
GCCTCTGGAA AATCCTCTTT TACTACCTTT GTGGACCTTG GAATGTACCA GCCATCAGGG 
GGTAGTGGAG ATAACATTTC TGTTGGAGGT CTGGGCTCTC GTTTTGAGCA GCTAAAGCTG 
GAGGTGAGGA AGGGCTCCAT GGTCAATGTC AATCCCACAA ACACACGTCC GGTCAGTGAC 
ACTCCTGAAA TCCGCAAATA CAAGAAGAGA TTTAACTCTG AGATCCTCTG CGCTGCTCTC 
TGGGGTGTGA ATCTGCTGGT CGGGACAGAG AATGGTCTGA AGCTGCTGGA CAGAAGCGGT 
CAGGGTAAAG TGTATCCACT GATCAACTCG CGCAGATTTC AGCAGATGGA TGTCCTGGAG 
GGACTGAACC TCCTCATCAC TATATCAGGC AAGAAGAATA AGGTACGCGT GTATTATCTG 
GCCTGGCTGA GGAATAAGAT CCTCCATAAT GACCCAGAGG TGGAGAAGAA ACAGGGCTGG 
ACCACTGTAG GGGAAATGGA GGGCTGTGTG CACTATAAAG TGGTGAAATA TGAAAGGATT 
AAGTTTCTGG TTATCGCTCT GAAGAATGCA GTGGAGGTTT ACGCCTGGGC TCCAAAACCT 
TACCACAAGT TCATGGCCTT CAAGTCATTT GGAGACCTGC CGCACAGACC TGTGTTGGTT 
GACCTCACAG TGGAAGAGGG TCAGAGGTTA AAGGTCATCT ATGGCTCCTT GGCTGGCTTC 
CATGCCATTG ATGTTGACTC TGGAAACAAC TACGACATCT ACATTCCTGT ACATATCCAG 
TCACAGATCA CTCCTCACGC CATCGTGTTC CTGCCCAACT CAGATGGGAT GGAGATGCTG 
TTGTGTTATG AGGACGAAGG TGTTTATGTC AACACGTACG GACGCATCAT TAAAGATGTG 
GTGCTGCAGT GGGGAGAAAT GCCCACATCA GTAGCCCACA TTTGCTCTAA TCAGATTATG 
GGTTGGGGAG AGAAAGCCAT TGAGATCCGT TCTGTTGAGA CGGGACACCT GGATGGAGTC 
TTCATGCACA AGAGAGCCCA GAGGCTTAAG TTCCTGTGTG AACGGAACGA CAAGGTGTTT 
TTCGCGTCAG TGCGTTCCGG TGGCAGCAGC CAAGTTTACT TCATGACCCT CAACAGAAAC 
TGCATTATGA ACTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	mink1
Entrez Gene ID	100038799
Full Name	misshapen-like kinase 1
Synonyms	msn3,si:ch211-147a23.2,zgc:162163
General protein information	Preferred Names misshapen-like kinase 1 Names misshapen-like kinase 1
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

mink1 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム