ORF » Species Summary » Danio rerio » SPG20A cDNA ORF clone

SPG20A cDNA ORF clone, Danio rerio(Zebrafish)

The following SPG20A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPG20A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa32855 NM_001113658.1
Latest version!		 Danio rerio spartin a (sparta), mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
ODa16197 XM_005172634.4
Latest version!		 Danio rerio spastic paraplegia 20a (Troyer syndrome) (spg20a), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
View Old Accession Versions >>
ODa16197 XM_005172634.3 Danio rerio spastic paraplegia 20a (Troyer syndrome) (spg20a), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    SPG20A ( NM_001113658.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> NP_001107130.1 Danio rerio spartin a (sparta), mRNA.
    Price & Availability↑
    CloneID ODa32855
    Clone ID Related Accession (Same CDS sequence) NM_001113658.1
    Accession Version NM_001113658.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1704bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-02-25
    Organism Danio rerio(zebrafish)
    Product spartin
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC154514.1. On Jan 20, 2008 this sequence version replaced XM_001344685.1. ##Evidence-Data-START## Transcript exon combination :: BC154514.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGCAGACGC CTGAACCCGC CGAAATTCGT GTCATTCGCG AGAACTACGA TAAAGCTCTG 
    CAGTGTCTGA ATACCGGACT TCAGTTAGAT GAAGCTGGCA ATAAAGACCA GGCTCTGGTC 
    CTGTACAGGC TGGGCCGGCG GCACCTGCTC AGTGGGTTGC AGGTGGACAC ACGGGGCGGG 
    CGCTGCACCG GCCGCTGCTG GGACTCTGCC CGACAAACGC AGCTGAAGAT GGGAGAGACT 
    CTGCGCACCA TCACTGCTCG CCTCGGGGCT CTAGAGTCCG CTTCAAACAC GGGTCAGAGA 
    CTTTATCCGA CTCTCCCTGT CTTCCAGGAC AAGAACTCCC AGAGAGCATT TAACAGCAGA 
    GGGGCCTCAG GAAGGGCCCC TTCTCGCCCT GTTTCCCCTC CAGTGGGCTT CGCGGTGCCT 
    AATGAACTCC CACCAGCCTA CACTCCTCAG CCAACAGACG GGCATCTGTC ACTCTCTCAT 
    GGAGCCAACA GAGCGTTTCC AGATGCCCCA CACCAGGGGT CCCGGCGTCA GGCTGTGGCC 
    CCGGTCAATC TCAGAGAGGC CGGGATGGAG ATTATGTTCT TGCCCAGCGG GGTGCAGATG 
    TTTTTTGTGT CTGCTGAGGG TCGGGTCACT GCACCTTCTT ATCCAGGGTA CCTGAGGATC 
    ATCATATACA GCCAGGACTC GGATGGCAGT GCCAACACTG GCTATGCTCC AGCTTACCTG 
    CAGGTTTGTG ACTGGATCTA CCCGCTGTAT CCAGACTTTC CTGTCTTGCT GAGCAACAAT 
    GGTGTGTTCA CCTTTCCTGA CACCACTGCA GCGGTGCCGG GGTCCTACGT TGGAGTGGTG 
    CTGTCCTCAG ATCTACCAGC AGGGGAGAGA GATCTGTTCC AGGAGCAGCT GTCAGTGTTG 
    GCGCAGCTCC GGGTCCAGGT AGATGAAGAA GGCGGCGGCG CTACAGGAGC AGACACAAAC 
    CTGAGTGAAA AGATTCCTCT ATCAGAAGCA TCTCAAACGC CAGAGGAGAA AACTGCCCCT 
    GTGTGGAGCG AAAAGATGTC ACAGAGCATC CTAGCAGGCT CCTCTTGGCT GGGTCGGGGT 
    CTTGTGCGAG GAGCTGAAGC GACTGGTAAA GCCATTCAGA GAGGGGGGAC TAAGCTGCGA 
    GACAACATCA CCCCAGAGGA GACTCCAGCA GAGGTCAGTC CTAAAGTCAC CAAAGGCCTC 
    AACGCAGCCA AACAGGCCAC TGGCGGAGCG GTCAAAGTCA GCCAGTTTCT GGTGGACGGT 
    GTTGCTGCCG TAGCGGGTAA AGTTGGGAAG GAACTCGCTC CACATGTAAA AAAACACGGT 
    TGCAAACTCA TCCCTGAGTC CCTCAAGAAA GGCAAAGATG GCAGCTCCAA AATGGATGGA 
    GCCAAACTCG TGGCTGGAAG CAGCATACAA GGTTTATCGA CTCTCTGGTC AAGTCTGGAA 
    ACAGCAGGAA AGACTATCGG GAAAAGTTTA TCATCAGAGA CGGTAACCAC TGTGAGGCAC 
    AAGTATGGAG ACGAGGCAGG ACAAGCGGCA GACACTGCAG TCCAGTCTAC TGTCAATGTG 
    GGGGTCACAG CATTTAATAT GGACAATGTG TGCCTCAAGG GTGTGCTGAA GGCCACAGGC 
    AAACAGACTA CAAAGGAAAT AGTCAAAGAC ACGCCAAAAG GAGAAAAAAC TGAGGACAAA 
    GACAAGTCAC CAAAGAAAAG ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001107130.1
    CDS78..1781
    Translation

    Target ORF information:

    RefSeq Version NM_001113658.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio spartin a (sparta), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001113658.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGCAGACGC CTGAACCCGC CGAAATTCGT GTCATTCGCG AGAACTACGA TAAAGCTCTG 
    CAGTGTCTGA ATACCGGACT TCAGTTAGAT GAAGCTGGCA ATAAAGACCA GGCTCTGGTC 
    CTGTACAGGC TGGGCCGGCG GCACCTGCTC AGTGGGTTGC AGGTGGACAC ACGGGGCGGG 
    CGCTGCACCG GCCGCTGCTG GGACTCTGCC CGACAAACGC AGCTGAAGAT GGGAGAGACT 
    CTGCGCACCA TCACTGCTCG CCTCGGGGCT CTAGAGTCCG CTTCAAACAC GGGTCAGAGA 
    CTTTATCCGA CTCTCCCTGT CTTCCAGGAC AAGAACTCCC AGAGAGCATT TAACAGCAGA 
    GGGGCCTCAG GAAGGGCCCC TTCTCGCCCT GTTTCCCCTC CAGTGGGCTT CGCGGTGCCT 
    AATGAACTCC CACCAGCCTA CACTCCTCAG CCAACAGACG GGCATCTGTC ACTCTCTCAT 
    GGAGCCAACA GAGCGTTTCC AGATGCCCCA CACCAGGGGT CCCGGCGTCA GGCTGTGGCC 
    CCGGTCAATC TCAGAGAGGC CGGGATGGAG ATTATGTTCT TGCCCAGCGG GGTGCAGATG 
    TTTTTTGTGT CTGCTGAGGG TCGGGTCACT GCACCTTCTT ATCCAGGGTA CCTGAGGATC 
    ATCATATACA GCCAGGACTC GGATGGCAGT GCCAACACTG GCTATGCTCC AGCTTACCTG 
    CAGGTTTGTG ACTGGATCTA CCCGCTGTAT CCAGACTTTC CTGTCTTGCT GAGCAACAAT 
    GGTGTGTTCA CCTTTCCTGA CACCACTGCA GCGGTGCCGG GGTCCTACGT TGGAGTGGTG 
    CTGTCCTCAG ATCTACCAGC AGGGGAGAGA GATCTGTTCC AGGAGCAGCT GTCAGTGTTG 
    GCGCAGCTCC GGGTCCAGGT AGATGAAGAA GGCGGCGGCG CTACAGGAGC AGACACAAAC 
    CTGAGTGAAA AGATTCCTCT ATCAGAAGCA TCTCAAACGC CAGAGGAGAA AACTGCCCCT 
    GTGTGGAGCG AAAAGATGTC ACAGAGCATC CTAGCAGGCT CCTCTTGGCT GGGTCGGGGT 
    CTTGTGCGAG GAGCTGAAGC GACTGGTAAA GCCATTCAGA GAGGGGGGAC TAAGCTGCGA 
    GACAACATCA CCCCAGAGGA GACTCCAGCA GAGGTCAGTC CTAAAGTCAC CAAAGGCCTC 
    AACGCAGCCA AACAGGCCAC TGGCGGAGCG GTCAAAGTCA GCCAGTTTCT GGTGGACGGT 
    GTTGCTGCCG TAGCGGGTAA AGTTGGGAAG GAACTCGCTC CACATGTAAA AAAACACGGT 
    TGCAAACTCA TCCCTGAGTC CCTCAAGAAA GGCAAAGATG GCAGCTCCAA AATGGATGGA 
    GCCAAACTCG TGGCTGGAAG CAGCATACAA GGTTTATCGA CTCTCTGGTC AAGTCTGGAA 
    ACAGCAGGAA AGACTATCGG GAAAAGTTTA TCATCAGAGA CGGTAACCAC TGTGAGGCAC 
    AAGTATGGAG ACGAGGCAGG ACAAGCGGCA GACACTGCAG TCCAGTCTAC TGTCAATGTG 
    GGGGTCACAG CATTTAATAT GGACAATGTG TGCCTCAAGG GTGTGCTGAA GGCCACAGGC 
    AAACAGACTA CAAAGGAAAT AGTCAAAGAC ACGCCAAAAG GAGAAAAAAC TGAGGACAAA 
    GACAAGTCAC CAAAGAAAAG ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    SPG20A ( XM_005172634.4 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005172691.1 Danio rerio spastic paraplegia 20a (Troyer syndrome) (spg20a), transcript variant X1, mRNA.
    Price & Availability↑
    CloneID ODa16197
    Clone ID Related Accession (Same CDS sequence) XM_005172634.4 , XM_005172634.3
    Accession Version XM_005172634.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1548bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product spartin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005172634.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGCAGACGC CTGAACCCGC CGAAATTCGT GTCATTCGCG AGAACTACGA TAAAGCTCTG 
    CAGTGTCTGA ATACCGGACT TCAGTTAGAT GAAGCTGGCA ATAAAGACCA GGCTCTGGTC 
    CTGTACAGGC TGGGCCGGCG GCACCTGCTC AGTGGGTTGC AGGTGGACAC ACGGGGCGGG 
    CGCTGCACCG GCCGCTGCTG GGACTCTGCC CGACAAACGC AGCTGAAGAT GGGAGAGACT 
    CTGCGCACCA TCACTGCTCG CCTCGGGGCT CTAGAGTCCG CTTCAAACAC GGGTCAGAGA 
    CTTTATCCGA CTCTCCCTGT CTTCCAGGAC AAGAACTCCC AGAGAGCATT TAACAGCAGA 
    GGGGCCTCAG GAAGGGCCCC TTCTCGCCCT GTTTCCCCTC CAGTGGGCTT CGCGGTGCCT 
    AATGAACTCC CACCAGCCTA CACTCCTCAG CCAACAGACG GGCATCTGTC ACTCTCTCAT 
    GGAGCCAACA GAGCGTTTCC AGATGCCCCA CACCAGGGGT CCCGGCGTCA GGCTGTGGCC 
    CCGGTCAATC TCAGAGAGGC CGGGATGGAG ATTATGTTCT TGCCCAGCGG GGTGCAGATG 
    TTTTTTGTGT CTGCTGAGGG TCGGGTCACT GCACCTTCTT ATCCAGGGTA CCTGAGGATC 
    ATCATATACA GCCAGGACTC GGATGGCAGT GCCAACACTG GCTATGCTCC AGCTTACCTG 
    CAGGTTTGTG ACTGGATCTA CCCGCTGTAT CCAGACTTTC CTGTCTTGCT GAGCAACAAT 
    GGTGTGTTCA CCTTTCCTGA CACCACTGCA GCGGTGCCGG GGTCCTACGT TGGAGTGGTG 
    CTGTCCTCAG ATCTACCAGC AGGGGAGAGA GATCTGTTCC AGGAGCAGCT GTCAGTGTTG 
    GCGCAGCTCC GGGTCCAGGT AGATGAAGAA GGCGGCGGCG CTACAGGAGC AGACACAAAC 
    CTGAGTGAAA AGATTCCTCT ATCAGAAGCA TCTCAAACGC CAGAGGAGAA AACTGCCCCT 
    GTGTGGAGCG AAAAGATGTC ACAGAGCATC CTAGCAGGCT CCTCTTGGCT GGGTCGGGGT 
    CTTGTGCGAG GAGCTGAAGC GACTGGTAAA GCCATTCAGA GAGGGGGGAC TAAGCTGCGA 
    GACAACATCA CCCCAGAGGA GACTCCAGCA GAGGTCAGTC CTAAAGTCAC CAAAGGCCTC 
    AACGCAGCCA AACAGGCCAC TGGCGGAGCG GTCAAAGTCA GCCAGTTTCT GGTGGACGGT 
    GTTGCTGCCG TAGCGGGTAA AGTTGGGAAG GAACTCGCTC CACATGTAAA AAAACACGGT 
    TGCAAACTCA TCCCTGAGTC CCTCAAGAAA GGCAAAGATG GCAGCTCCAA AATGGATGGA 
    GCCAAACTCG TGGCTGGAAG CAGCATACAA GGTTTATCGA CTCTCTGGTC AAGTCTGGAA 
    ACAGCAGGAA AGACTATCGG GAAAAGTTTA TCATCAGAGA CGGTAACCAC TGTGAGGCAC 
    AAATTTAGGG GTCTAGGGAG TATCTTGGAC CCTCCTAGTG GTGTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005172691.1
    CDS355..1902
    Translation

    Target ORF information:

    RefSeq Version XM_005172634.4
    Organism Danio rerio(zebrafish)
    Definition Danio rerio spastic paraplegia 20a (Troyer syndrome) (spg20a), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_005172634.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGCAGACGC CTGAACCCGC CGAAATTCGT GTCATTCGCG AGAACTACGA TAAAGCTCTG 
    CAGTGTCTGA ATACCGGACT TCAGTTAGAT GAAGCTGGCA ATAAAGACCA GGCTCTGGTC 
    CTGTACAGGC TGGGCCGGCG GCACCTGCTC AGTGGGTTGC AGGTGGACAC ACGGGGCGGG 
    CGCTGCACCG GCCGCTGCTG GGACTCTGCC CGACAAACGC AGCTGAAGAT GGGAGAGACT 
    CTGCGCACCA TCACTGCTCG CCTCGGGGCT CTAGAGTCCG CTTCAAACAC GGGTCAGAGA 
    CTTTATCCGA CTCTCCCTGT CTTCCAGGAC AAGAACTCCC AGAGAGCATT TAACAGCAGA 
    GGGGCCTCAG GAAGGGCCCC TTCTCGCCCT GTTTCCCCTC CAGTGGGCTT CGCGGTGCCT 
    AATGAACTCC CACCAGCCTA CACTCCTCAG CCAACAGACG GGCATCTGTC ACTCTCTCAT 
    GGAGCCAACA GAGCGTTTCC AGATGCCCCA CACCAGGGGT CCCGGCGTCA GGCTGTGGCC 
    CCGGTCAATC TCAGAGAGGC CGGGATGGAG ATTATGTTCT TGCCCAGCGG GGTGCAGATG 
    TTTTTTGTGT CTGCTGAGGG TCGGGTCACT GCACCTTCTT ATCCAGGGTA CCTGAGGATC 
    ATCATATACA GCCAGGACTC GGATGGCAGT GCCAACACTG GCTATGCTCC AGCTTACCTG 
    CAGGTTTGTG ACTGGATCTA CCCGCTGTAT CCAGACTTTC CTGTCTTGCT GAGCAACAAT 
    GGTGTGTTCA CCTTTCCTGA CACCACTGCA GCGGTGCCGG GGTCCTACGT TGGAGTGGTG 
    CTGTCCTCAG ATCTACCAGC AGGGGAGAGA GATCTGTTCC AGGAGCAGCT GTCAGTGTTG 
    GCGCAGCTCC GGGTCCAGGT AGATGAAGAA GGCGGCGGCG CTACAGGAGC AGACACAAAC 
    CTGAGTGAAA AGATTCCTCT ATCAGAAGCA TCTCAAACGC CAGAGGAGAA AACTGCCCCT 
    GTGTGGAGCG AAAAGATGTC ACAGAGCATC CTAGCAGGCT CCTCTTGGCT GGGTCGGGGT 
    CTTGTGCGAG GAGCTGAAGC GACTGGTAAA GCCATTCAGA GAGGGGGGAC TAAGCTGCGA 
    GACAACATCA CCCCAGAGGA GACTCCAGCA GAGGTCAGTC CTAAAGTCAC CAAAGGCCTC 
    AACGCAGCCA AACAGGCCAC TGGCGGAGCG GTCAAAGTCA GCCAGTTTCT GGTGGACGGT 
    GTTGCTGCCG TAGCGGGTAA AGTTGGGAAG GAACTCGCTC CACATGTAAA AAAACACGGT 
    TGCAAACTCA TCCCTGAGTC CCTCAAGAAA GGCAAAGATG GCAGCTCCAA AATGGATGGA 
    GCCAAACTCG TGGCTGGAAG CAGCATACAA GGTTTATCGA CTCTCTGGTC AAGTCTGGAA 
    ACAGCAGGAA AGACTATCGG GAAAAGTTTA TCATCAGAGA CGGTAACCAC TGTGAGGCAC 
    AAATTTAGGG GTCTAGGGAG TATCTTGGAC CCTCCTAGTG GTGTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    SPG20A ( XM_005172634.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005172691.1 Danio rerio spastic paraplegia 20a (Troyer syndrome) (spg20a), transcript variant X1, mRNA.
    Price & Availability↑
    CloneID ODa16197
    Clone ID Related Accession (Same CDS sequence) XM_005172634.4 , XM_005172634.3
    Accession Version XM_005172634.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1548bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product spartin isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005172634.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGCAGACGC CTGAACCCGC CGAAATTCGT GTCATTCGCG AGAACTACGA TAAAGCTCTG 
    CAGTGTCTGA ATACCGGACT TCAGTTAGAT GAAGCTGGCA ATAAAGACCA GGCTCTGGTC 
    CTGTACAGGC TGGGCCGGCG GCACCTGCTC AGTGGGTTGC AGGTGGACAC ACGGGGCGGG 
    CGCTGCACCG GCCGCTGCTG GGACTCTGCC CGACAAACGC AGCTGAAGAT GGGAGAGACT 
    CTGCGCACCA TCACTGCTCG CCTCGGGGCT CTAGAGTCCG CTTCAAACAC GGGTCAGAGA 
    CTTTATCCGA CTCTCCCTGT CTTCCAGGAC AAGAACTCCC AGAGAGCATT TAACAGCAGA 
    GGGGCCTCAG GAAGGGCCCC TTCTCGCCCT GTTTCCCCTC CAGTGGGCTT CGCGGTGCCT 
    AATGAACTCC CACCAGCCTA CACTCCTCAG CCAACAGACG GGCATCTGTC ACTCTCTCAT 
    GGAGCCAACA GAGCGTTTCC AGATGCCCCA CACCAGGGGT CCCGGCGTCA GGCTGTGGCC 
    CCGGTCAATC TCAGAGAGGC CGGGATGGAG ATTATGTTCT TGCCCAGCGG GGTGCAGATG 
    TTTTTTGTGT CTGCTGAGGG TCGGGTCACT GCACCTTCTT ATCCAGGGTA CCTGAGGATC 
    ATCATATACA GCCAGGACTC GGATGGCAGT GCCAACACTG GCTATGCTCC AGCTTACCTG 
    CAGGTTTGTG ACTGGATCTA CCCGCTGTAT CCAGACTTTC CTGTCTTGCT GAGCAACAAT 
    GGTGTGTTCA CCTTTCCTGA CACCACTGCA GCGGTGCCGG GGTCCTACGT TGGAGTGGTG 
    CTGTCCTCAG ATCTACCAGC AGGGGAGAGA GATCTGTTCC AGGAGCAGCT GTCAGTGTTG 
    GCGCAGCTCC GGGTCCAGGT AGATGAAGAA GGCGGCGGCG CTACAGGAGC AGACACAAAC 
    CTGAGTGAAA AGATTCCTCT ATCAGAAGCA TCTCAAACGC CAGAGGAGAA AACTGCCCCT 
    GTGTGGAGCG AAAAGATGTC ACAGAGCATC CTAGCAGGCT CCTCTTGGCT GGGTCGGGGT 
    CTTGTGCGAG GAGCTGAAGC GACTGGTAAA GCCATTCAGA GAGGGGGGAC TAAGCTGCGA 
    GACAACATCA CCCCAGAGGA GACTCCAGCA GAGGTCAGTC CTAAAGTCAC CAAAGGCCTC 
    AACGCAGCCA AACAGGCCAC TGGCGGAGCG GTCAAAGTCA GCCAGTTTCT GGTGGACGGT 
    GTTGCTGCCG TAGCGGGTAA AGTTGGGAAG GAACTCGCTC CACATGTAAA AAAACACGGT 
    TGCAAACTCA TCCCTGAGTC CCTCAAGAAA GGCAAAGATG GCAGCTCCAA AATGGATGGA 
    GCCAAACTCG TGGCTGGAAG CAGCATACAA GGTTTATCGA CTCTCTGGTC AAGTCTGGAA 
    ACAGCAGGAA AGACTATCGG GAAAAGTTTA TCATCAGAGA CGGTAACCAC TGTGAGGCAC 
    AAATTTAGGG GTCTAGGGAG TATCTTGGAC CCTCCTAGTG GTGTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005172691.1
    CDS852..2399
    Translation

    Target ORF information:

    RefSeq Version XM_005172634.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio spastic paraplegia 20a (Troyer syndrome) (spg20a), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_005172634.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGCAGACGC CTGAACCCGC CGAAATTCGT GTCATTCGCG AGAACTACGA TAAAGCTCTG 
    CAGTGTCTGA ATACCGGACT TCAGTTAGAT GAAGCTGGCA ATAAAGACCA GGCTCTGGTC 
    CTGTACAGGC TGGGCCGGCG GCACCTGCTC AGTGGGTTGC AGGTGGACAC ACGGGGCGGG 
    CGCTGCACCG GCCGCTGCTG GGACTCTGCC CGACAAACGC AGCTGAAGAT GGGAGAGACT 
    CTGCGCACCA TCACTGCTCG CCTCGGGGCT CTAGAGTCCG CTTCAAACAC GGGTCAGAGA 
    CTTTATCCGA CTCTCCCTGT CTTCCAGGAC AAGAACTCCC AGAGAGCATT TAACAGCAGA 
    GGGGCCTCAG GAAGGGCCCC TTCTCGCCCT GTTTCCCCTC CAGTGGGCTT CGCGGTGCCT 
    AATGAACTCC CACCAGCCTA CACTCCTCAG CCAACAGACG GGCATCTGTC ACTCTCTCAT 
    GGAGCCAACA GAGCGTTTCC AGATGCCCCA CACCAGGGGT CCCGGCGTCA GGCTGTGGCC 
    CCGGTCAATC TCAGAGAGGC CGGGATGGAG ATTATGTTCT TGCCCAGCGG GGTGCAGATG 
    TTTTTTGTGT CTGCTGAGGG TCGGGTCACT GCACCTTCTT ATCCAGGGTA CCTGAGGATC 
    ATCATATACA GCCAGGACTC GGATGGCAGT GCCAACACTG GCTATGCTCC AGCTTACCTG 
    CAGGTTTGTG ACTGGATCTA CCCGCTGTAT CCAGACTTTC CTGTCTTGCT GAGCAACAAT 
    GGTGTGTTCA CCTTTCCTGA CACCACTGCA GCGGTGCCGG GGTCCTACGT TGGAGTGGTG 
    CTGTCCTCAG ATCTACCAGC AGGGGAGAGA GATCTGTTCC AGGAGCAGCT GTCAGTGTTG 
    GCGCAGCTCC GGGTCCAGGT AGATGAAGAA GGCGGCGGCG CTACAGGAGC AGACACAAAC 
    CTGAGTGAAA AGATTCCTCT ATCAGAAGCA TCTCAAACGC CAGAGGAGAA AACTGCCCCT 
    GTGTGGAGCG AAAAGATGTC ACAGAGCATC CTAGCAGGCT CCTCTTGGCT GGGTCGGGGT 
    CTTGTGCGAG GAGCTGAAGC GACTGGTAAA GCCATTCAGA GAGGGGGGAC TAAGCTGCGA 
    GACAACATCA CCCCAGAGGA GACTCCAGCA GAGGTCAGTC CTAAAGTCAC CAAAGGCCTC 
    AACGCAGCCA AACAGGCCAC TGGCGGAGCG GTCAAAGTCA GCCAGTTTCT GGTGGACGGT 
    GTTGCTGCCG TAGCGGGTAA AGTTGGGAAG GAACTCGCTC CACATGTAAA AAAACACGGT 
    TGCAAACTCA TCCCTGAGTC CCTCAAGAAA GGCAAAGATG GCAGCTCCAA AATGGATGGA 
    GCCAAACTCG TGGCTGGAAG CAGCATACAA GGTTTATCGA CTCTCTGGTC AAGTCTGGAA 
    ACAGCAGGAA AGACTATCGG GAAAAGTTTA TCATCAGAGA CGGTAACCAC TGTGAGGCAC 
    AAATTTAGGG GTCTAGGGAG TATCTTGGAC CCTCCTAGTG GTGTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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