SPART cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	SPART
Entrez Gene ID	534027
Full Name	spartin
Synonyms	SPG20
General protein information	Preferred Names spartin Names spartin spastic paraplegia 20 (Troyer syndrome)
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005213643.3	XP_005213700.1	spartin isoform X3
XM_005213641.4	XP_005213698.1	spartin isoform X1
XM_005213643.4	XP_005213700.1	spartin isoform X3
XM_005213641.3	XP_005213698.1	spartin isoform X1
XM_010810718.2	XP_010809020.1	spartin isoform X1
XM_010810718.3	XP_010809020.1	spartin isoform X1
NM_001077996.1	NP_001071464.1	spartin
XM_005213642.3	XP_005213699.1	spartin isoform X2

Pathways from BioSystems

Homologies

	SPG20	XP_001083494.1
Canis lupus familiaris (dog)	SPG20	XP_005635515.1
Gallus gallus (chicken)	SPG20	XP_004938806.1
Rattus norvegicus (Norway rat)	Spg20	NP_001099903.2
Danio rerio (zebrafish)	spg20a	NP_001107130.1
Caenorhabditis elegans (roundworm)	F57B10.9	NP_491888.2
Xenopus tropicalis (tropical clawed frog)	spg20	NP_001123727.1
Homo sapiens (human)	SPG20	NP_001135766.1
Bos taurus (cattle)	SPG20	NP_001071464.1
Pan troglodytes (chimpanzee)	SPG20	XP_003314174.1

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following SPART gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPART cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa42136	NM_001077996.1 Latest version!	Bos taurus spartin (SPART), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OBa24680	XM_005213641.4 Latest version!	Bos taurus spartin (SPART), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OBa24680	XM_010810718.3 Latest version!	Bos taurus spartin (SPART), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OBa24681	XM_005213642.3 Latest version!	Bos taurus spartin (SPART), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OBa24682	XM_005213643.4 Latest version!	Bos taurus spartin (SPART), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
View Old Accession Versions >>
OBa24682	XM_005213643.3	Bos taurus spastic paraplegia 20 (Troyer syndrome) (SPG20), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OBa24680	XM_005213641.3	Bos taurus spastic paraplegia 20 (Troyer syndrome) (SPG20), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OBa24680	XM_010810718.2	Bos taurus spastic paraplegia 20 (Troyer syndrome) (SPG20), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SPART ( NM_001077996.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001071464.1 Bos taurus spartin (SPART), mRNA.

Price & Availability↑

CloneID	OBa42136
Clone ID Related Accession (Same CDS sequence)	NM_001077996.1
Accession Version	NM_001077996.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2007bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-18
Organism	Bos taurus(cattle)
Product	spartin
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC126710.1. On Nov 21, 2006 this sequence version replaced XM_613638.2. ##Evidence-Data-START## Transcript exon combination :: BC126710.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145412, SAMN03145413 [ECO:0000348] ##Evidence-Data-END##

ATGGAACAAG GACCCCAAGA TGGAGAACCT ATTGAAATTA AGATCATCAA GGAAGCATAC 
AAGAAAGCCT TTGTATTTGT TAATAAAGGA CTGAATACAG ATGAATTAGG TCAAAAGGAA 
GAAGCAAAGA ACTACTATAA GCAAGGAATA GGACACCTGC TTAGAGGAAT CAGCATTTCA 
TCAACAGATC CTGAGTACAC AGGTCCTGAG TGGGAGTCTG CCAGGCAGAT GCAACAGAAA 
ATGAAAGAAA CGCTACAGAA TGTACGTACC AGGTTGGAAA TTCTAGAGAA GGGTCTTGCC 
ACTTCTCTAC GGAATGATCT TCAGGAGGTG CCCAAGTTGT ATCCAGAATT TCCACCTAAA 
GACATGTCTG AAAAGTCACC AGAGCCTCAG AGCCTTAGTT CACTTCCTCA GCACAGTGAA 
GTAAATGGCA GCACTTCAAC TGCAAGTGCA GAATCAAGTA GTACACCTAC TACTTTGTCC 
TTGCCGTGTC AGAGTCATCC GTCCGAAGCA CCTCCTGCTT ACACTCCCCA GGCTGCTGAG 
GGTCACTACA CTGTATCCTA TGGAACAGAA TCTGGGGAGT TCTCGTCAGT TGGGGAGAAC 
TTCTATAGGA ATCACTCTCA GCCACCTCCT CTTGAGACCT TAGGGCTGGA TGCAGATGAG 
TTGATTTTGA TACCAAACGG AGTACAAATT TTCTTTGTAA ATCCCGCAGG GGAGGTTAGT 
GCACCTTCAT ATCCTGGGTA CCTTCGAATC GTGAGGTTCT TGGATAATTC TCTTGATACC 
TTTCTAAATC GTCCTCCTGG ATTTCTCCAG GTTTGTGACT GGTTGTATCC TCTAGTTCCT 
GATAGGTCTC CAGTTCTTAA ATGTACTGTA GGTGCCTACA TGTTTCCTGA TACGATGTTA 
CAAGCATCGG GATGCTTTGT GGGGGTCGTC TTGTCATCTG AATTACCAGA AGATGACAGA 
GAACTCTTCG AGGATCTATT AAGACAAATG TCTGACCTTC GGCTCCAGAC CAACTGGGAC 
CGGGCAGAAG GAGAAAATGA ATTCCAGATC CCTGGAATAT CAGGATCTGC CTCTGACCAG 
TTGAAGGAAG CCAGTGGCAC TGACGTGAGA CAGTTGGACC CAAGCAGTAA AGATGTGCGT 
CAGAAGGGAA AACGAGGGAA AAAGACCAAA GGAACTTCAA GTGAAGAAGT TAATCTGAGT 
CACATTGTAC CCTGTGAGCC AGTTTCAGAA GAAAAAGCAA AGGAGTTACC TGAATGGAGT 
GAGAAAGTGG CTCACAACAT TTTGTCAGGT GCTTCCTGGG TGAGTTGGGG TTTGGTCAAA 
GGTGCTGAGT TTACTGGCAA AGCCATCCAG AAAGGAGCGT CTAAACTTCG AGAACGAATT 
CAACCAGAAG AAAAGCCTGT GGAAGTTAGT CCAGCTGTGA CCAAGGGACT TTATATGGCA 
AAGCAGGCGA CTGGAGGAGC AGCCAAAGTC AGTCAGTTTC TGGTTGATGG AGTTTGTACT 
GTGGCAAATT GTGTTGGAAA GGAATTAGCT CCACACGTCA AGAAGCACGG AAGCAAACTT 
GTTCCAGAAT CTCTTAAAAA AGACCGAAAT GGAAAGTCTA CCCTGGATGG CGCTATGGTT 
GTAGCAGCAA GTAGTGTTCA AGGATTCTCA ACTGTCTGGC AAGGATTGGA ATGTGCAGCT 
AAATGCATTG TTAACAATGT TTCAGCAGAA ACTGTACAAA CTGTCAGATA CAAATATGGG 
CATACTGCAG GAGAAGCTAC ACACAACGCA GTAGATTCTG CCATCAATGT TGGTGTAACT 
GCCTATAATA TTGACAACAT TGGCATCAAA GCAATGGTGA AGAAAACTGC AAAACAAACA 
GGACACACAC TGCTTGAGGA CTATAAAATC ATTGATAATT CTAAGGGGGA AAATCCAGGA 
GGAGGAGCAA GTGCAAACCT GAAAGGGGAG AAAGATGAGC AGAAAGAGGG GCCAGAGAAG 
AACGGGGCAA AGAAGAAAGA TAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001071464.1
CDS	142..2148
Translation	MEQGPQDGEPIEIKIIKEAYKKAFVFVNKGLNTDELGQKEEAKNYYKQGIGHLLRGISISSTDPEYTGPEWESARQMQQKMKETLQNVRTRLEILEKGLATSLRNDLQEVPKLYPEFPPKDMSEKSPEPQSLSSLPQHSEVNGSTSTASAESSSTPTTLSLPCQSHPSEAPPAYTPQAAEGHYTVSYGTESGEFSSVGENFYRNHSQPPPLETLGLDADELILIPNGVQIFFVNPAGEVSAPSYPGYLRIVRFLDNSLDTFLNRPPGFLQVCDWLYPLVPDRSPVLKCTVGAYMFPDTMLQASGCFVGVVLSSELPEDDRELFEDLLRQMSDLRLQTNWDRAEGENEFQIPGISGSASDQLKEASGTDVRQLDPSSKDVRQKGKRGKKTKGTSSEEVNLSHIVPCEPVSEEKAKELPEWSEKVAHNILSGASWVSWGLVKGAEFTGKAIQKGASKLRERIQPEEKPVEVSPAVTKGLYMAKQATGGAAKVSQFLVDGVCTVANCVGKELAPHVKKHGSKLVPESLKKDRNGKSTLDGAMVVAASSVQGFSTVWQGLECAAKCIVNNVSAETVQTVRYKYGHTAGEATHNAVDSAINVGVTAYNIDNIGIKAMVKKTAKQTGHTLLEDYKIIDNSKGENPGGGASANLKGEKDEQKEGPEKNGAKKKDK

Target ORF information:

RefSeq Version	NM_001077996.1
Organism	Bos taurus(cattle)
Definition	Bos taurus spartin (SPART), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001077996.1

ORF Insert Sequence:

ATGGAACAAG GACCCCAAGA TGGAGAACCT ATTGAAATTA AGATCATCAA GGAAGCATAC 
AAGAAAGCCT TTGTATTTGT TAATAAAGGA CTGAATACAG ATGAATTAGG TCAAAAGGAA 
GAAGCAAAGA ACTACTATAA GCAAGGAATA GGACACCTGC TTAGAGGAAT CAGCATTTCA 
TCAACAGATC CTGAGTACAC AGGTCCTGAG TGGGAGTCTG CCAGGCAGAT GCAACAGAAA 
ATGAAAGAAA CGCTACAGAA TGTACGTACC AGGTTGGAAA TTCTAGAGAA GGGTCTTGCC 
ACTTCTCTAC GGAATGATCT TCAGGAGGTG CCCAAGTTGT ATCCAGAATT TCCACCTAAA 
GACATGTCTG AAAAGTCACC AGAGCCTCAG AGCCTTAGTT CACTTCCTCA GCACAGTGAA 
GTAAATGGCA GCACTTCAAC TGCAAGTGCA GAATCAAGTA GTACACCTAC TACTTTGTCC 
TTGCCGTGTC AGAGTCATCC GTCCGAAGCA CCTCCTGCTT ACACTCCCCA GGCTGCTGAG 
GGTCACTACA CTGTATCCTA TGGAACAGAA TCTGGGGAGT TCTCGTCAGT TGGGGAGAAC 
TTCTATAGGA ATCACTCTCA GCCACCTCCT CTTGAGACCT TAGGGCTGGA TGCAGATGAG 
TTGATTTTGA TACCAAACGG AGTACAAATT TTCTTTGTAA ATCCCGCAGG GGAGGTTAGT 
GCACCTTCAT ATCCTGGGTA CCTTCGAATC GTGAGGTTCT TGGATAATTC TCTTGATACC 
TTTCTAAATC GTCCTCCTGG ATTTCTCCAG GTTTGTGACT GGTTGTATCC TCTAGTTCCT 
GATAGGTCTC CAGTTCTTAA ATGTACTGTA GGTGCCTACA TGTTTCCTGA TACGATGTTA 
CAAGCATCGG GATGCTTTGT GGGGGTCGTC TTGTCATCTG AATTACCAGA AGATGACAGA 
GAACTCTTCG AGGATCTATT AAGACAAATG TCTGACCTTC GGCTCCAGAC CAACTGGGAC 
CGGGCAGAAG GAGAAAATGA ATTCCAGATC CCTGGAATAT CAGGATCTGC CTCTGACCAG 
TTGAAGGAAG CCAGTGGCAC TGACGTGAGA CAGTTGGACC CAAGCAGTAA AGATGTGCGT 
CAGAAGGGAA AACGAGGGAA AAAGACCAAA GGAACTTCAA GTGAAGAAGT TAATCTGAGT 
CACATTGTAC CCTGTGAGCC AGTTTCAGAA GAAAAAGCAA AGGAGTTACC TGAATGGAGT 
GAGAAAGTGG CTCACAACAT TTTGTCAGGT GCTTCCTGGG TGAGTTGGGG TTTGGTCAAA 
GGTGCTGAGT TTACTGGCAA AGCCATCCAG AAAGGAGCGT CTAAACTTCG AGAACGAATT 
CAACCAGAAG AAAAGCCTGT GGAAGTTAGT CCAGCTGTGA CCAAGGGACT TTATATGGCA 
AAGCAGGCGA CTGGAGGAGC AGCCAAAGTC AGTCAGTTTC TGGTTGATGG AGTTTGTACT 
GTGGCAAATT GTGTTGGAAA GGAATTAGCT CCACACGTCA AGAAGCACGG AAGCAAACTT 
GTTCCAGAAT CTCTTAAAAA AGACCGAAAT GGAAAGTCTA CCCTGGATGG CGCTATGGTT 
GTAGCAGCAA GTAGTGTTCA AGGATTCTCA ACTGTCTGGC AAGGATTGGA ATGTGCAGCT 
AAATGCATTG TTAACAATGT TTCAGCAGAA ACTGTACAAA CTGTCAGATA CAAATATGGG 
CATACTGCAG GAGAAGCTAC ACACAACGCA GTAGATTCTG CCATCAATGT TGGTGTAACT 
GCCTATAATA TTGACAACAT TGGCATCAAA GCAATGGTGA AGAAAACTGC AAAACAAACA 
GGACACACAC TGCTTGAGGA CTATAAAATC ATTGATAATT CTAAGGGGGA AAATCCAGGA 
GGAGGAGCAA GTGCAAACCT GAAAGGGGAG AAAGATGAGC AGAAAGAGGG GCCAGAGAAG 
AACGGGGCAA AGAAGAAAGA TAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPART ( XM_005213641.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005213698.1 Bos taurus spartin (SPART), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa24680
Clone ID Related Accession (Same CDS sequence)	XM_005213641.4 , XM_005213641.3 , XM_010810718.2 , XM_010810718.3
Accession Version	XM_005213641.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2121bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	spartin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037339.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005213641.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAACAAG GACCCCAAGA TGGAGAACCT ATTGAAATTA AGATCATCAA GGAAGCATAC 
AAGAAAGCCT TTGTATTTGT TAATAAAGGA CTGAATACAG ATGAATTAGG TCAAAAGGAA 
GAAGCAAAGA ACTACTATAA GCAAGGAATA GGACACCTGC TTAGAGGAAT CAGCATTTCA 
TCAACAGATC CTGAGTACAC AGGTCCTGAG TGGGAGTCTG CCAGGCAGAT GCAACAGAAA 
ATGAAAGAAA CGCTACAGAA TGTACGTACC AGGTTGGAAA TTCTAGAGAA GGGTCTTGCC 
ACTTCTCTAC GGAATGATCT TCAGGAGGTG CCCAAGTTGT ATCCAGAATT TCCACCTAAA 
GACATGTCTG AAAAGTCACC AGAGCCTCAG AGCCTTAGTT CACTTCCTCA GCACAGTGAA 
GTAAATGGCA GCACTTCAAC TGCAAGTGCA GAATCAAGTA GTACACCTAC TACTTTGTCC 
TTGCCGTGTC AGAGTCATCC GTCCGAAGCA CCTCCTGCTT ACACTCCCCA GGCTGCTGAG 
GGTCACTACA CTGTATCCTA TGGAACAGAA TCTGGGGAGT TCTCGTCAGT TGGGGAGAAC 
TTCTATAGGA ATCACTCTCA GCCACCTCCT CTTGAGACCT TAGGGCTGGA TGCAGATGAG 
TTGATTTTGA TACCAAACGG AGTACAAATT TTCTTTGTAA ATCCCGCAGG GGAGGTTAGT 
GCACCTTCAT ATCCTGGGTA CCTTCGAATC GTGAGGTTCT TGGATAATTC TCTTGATACC 
TTTCTAAATC GTCCTCCTGG ATTTCTCCAG ATTTGTCGTC AATTCATATG GAACTTGGAT 
ATGAAGTTGC TCTGTGAAAG TGAAACTGGG AGAAATCAGC CTGTGGTTTG TGACTGGTTG 
TATCCTCTAG TTCCTGATAG GTCTCCAGTT CTTAAATGTA CTGTAGGTGC CTACATGTTT 
CCTGATACGA TGTTACAAGC ATCGGGATGC TTTGTGGGGG TCGTCTTGTC ATCTGAATTA 
CCAGAAGATG ACAGAGAACT CTTCGAGGAT CTATTAAGAC AAATGTCTGA CCTTCGGCTC 
CAGACCAACT GGGACCGGGC AGAAGGAGAA AATGAATTCC AGATCCCTGG AATATCAGGA 
TCTGCCTCTG ACCAGTTGAA GGAAGCCAGT GGCACTGACG TGAGACAGTT GGACCCAAGC 
AGTAAAGATG TGCGTCAGAA GGGAAAACGA GGGAAAAAGA TTCTGTGTTG TGTACCAAAT 
GTTGAATTTG GATTAGAGAC CAAAGGAACT TCAAGTGAAG AAGTTAATCT GAGTCACATT 
GTACCCTGTG AGCCAGTTTC AGAAGAAAAA GCAAAGGAGT TACCTGAATG GAGTGAGAAA 
GTGGCTCACA ACATTTTGTC AGGTGCTTCC TGGGTGAGTT GGGGTTTGGT CAAAGGTGCT 
GAGTTTACTG GCAAAGCCAT CCAGAAAGGA GCGTCTAAAC TTCGAGAACG AATTCAACCA 
GAAGAAAAGC CTGTGGAAGT TAGTCCAGCT GTGACCAAGG GACTTTATAT GGCAAAGCAG 
GCGACTGGAG GAGCAGCCAA AGTCAGTCAG TTTCTGGTTG ATGGAGTTTG TACTGTGGCA 
AATTGTGTTG GAAAGGAATT AGCTCCACAC GTCAAGAAGC ACGGAAGCAA ACTTGTTCCA 
GAATCTCTTA AAAAAGACCG AAATGGAAAG TCTACCCTGG ATGGCGCTAT GGTTGTAGCA 
GCAAGTAGTG TTCAAGGATT CTCAACTGTC TGGCAAGGAT TGGAATGTGC AGCTAAATGC 
ATTGTTAACA ATGTTTCAGC AGAAACTGTA CAAACTGTCA GATACAAATA TGGGCATACT 
GCAGGAGAAG CTACACACAA CGCAGTAGAT TCTGCCATCA ATGTTGGTGT AACTGCCTAT 
AATATTGACA ACATTGGCAT CAAAGCAATG GTGAAGAAAA CTGCAAAACA AACAGGACAC 
ACACTGCTTG AGGACTATAA AATCATTGAT AATTCTAAGG GGGAAAATCC AGGAGGAGGA 
GCAAGTGCAA ACCTGAAAGG GGAGAAAGAT GAGCAGAAAG AGGGGCCAGA GAAGAACGGG 
GCAAAGAAGA AAGATAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005213698.1
CDS	189..2309
Translation	MEQGPQDGEPIEIKIIKEAYKKAFVFVNKGLNTDELGQKEEAKNYYKQGIGHLLRGISISSTDPEYTGPEWESARQMQQKMKETLQNVRTRLEILEKGLATSLRNDLQEVPKLYPEFPPKDMSEKSPEPQSLSSLPQHSEVNGSTSTASAESSSTPTTLSLPCQSHPSEAPPAYTPQAAEGHYTVSYGTESGEFSSVGENFYRNHSQPPPLETLGLDADELILIPNGVQIFFVNPAGEVSAPSYPGYLRIVRFLDNSLDTFLNRPPGFLQICRQFIWNLDMKLLCESETGRNQPVVCDWLYPLVPDRSPVLKCTVGAYMFPDTMLQASGCFVGVVLSSELPEDDRELFEDLLRQMSDLRLQTNWDRAEGENEFQIPGISGSASDQLKEASGTDVRQLDPSSKDVRQKGKRGKKILCCVPNVEFGLETKGTSSEEVNLSHIVPCEPVSEEKAKELPEWSEKVAHNILSGASWVSWGLVKGAEFTGKAIQKGASKLRERIQPEEKPVEVSPAVTKGLYMAKQATGGAAKVSQFLVDGVCTVANCVGKELAPHVKKHGSKLVPESLKKDRNGKSTLDGAMVVAASSVQGFSTVWQGLECAAKCIVNNVSAETVQTVRYKYGHTAGEATHNAVDSAINVGVTAYNIDNIGIKAMVKKTAKQTGHTLLEDYKIIDNSKGENPGGGASANLKGEKDEQKEGPEKNGAKKKDK

Target ORF information:

RefSeq Version	XM_005213641.4
Organism	Bos taurus(cattle)
Definition	Bos taurus spartin (SPART), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005213641.4

ORF Insert Sequence:

ATGGAACAAG GACCCCAAGA TGGAGAACCT ATTGAAATTA AGATCATCAA GGAAGCATAC 
AAGAAAGCCT TTGTATTTGT TAATAAAGGA CTGAATACAG ATGAATTAGG TCAAAAGGAA 
GAAGCAAAGA ACTACTATAA GCAAGGAATA GGACACCTGC TTAGAGGAAT CAGCATTTCA 
TCAACAGATC CTGAGTACAC AGGTCCTGAG TGGGAGTCTG CCAGGCAGAT GCAACAGAAA 
ATGAAAGAAA CGCTACAGAA TGTACGTACC AGGTTGGAAA TTCTAGAGAA GGGTCTTGCC 
ACTTCTCTAC GGAATGATCT TCAGGAGGTG CCCAAGTTGT ATCCAGAATT TCCACCTAAA 
GACATGTCTG AAAAGTCACC AGAGCCTCAG AGCCTTAGTT CACTTCCTCA GCACAGTGAA 
GTAAATGGCA GCACTTCAAC TGCAAGTGCA GAATCAAGTA GTACACCTAC TACTTTGTCC 
TTGCCGTGTC AGAGTCATCC GTCCGAAGCA CCTCCTGCTT ACACTCCCCA GGCTGCTGAG 
GGTCACTACA CTGTATCCTA TGGAACAGAA TCTGGGGAGT TCTCGTCAGT TGGGGAGAAC 
TTCTATAGGA ATCACTCTCA GCCACCTCCT CTTGAGACCT TAGGGCTGGA TGCAGATGAG 
TTGATTTTGA TACCAAACGG AGTACAAATT TTCTTTGTAA ATCCCGCAGG GGAGGTTAGT 
GCACCTTCAT ATCCTGGGTA CCTTCGAATC GTGAGGTTCT TGGATAATTC TCTTGATACC 
TTTCTAAATC GTCCTCCTGG ATTTCTCCAG ATTTGTCGTC AATTCATATG GAACTTGGAT 
ATGAAGTTGC TCTGTGAAAG TGAAACTGGG AGAAATCAGC CTGTGGTTTG TGACTGGTTG 
TATCCTCTAG TTCCTGATAG GTCTCCAGTT CTTAAATGTA CTGTAGGTGC CTACATGTTT 
CCTGATACGA TGTTACAAGC ATCGGGATGC TTTGTGGGGG TCGTCTTGTC ATCTGAATTA 
CCAGAAGATG ACAGAGAACT CTTCGAGGAT CTATTAAGAC AAATGTCTGA CCTTCGGCTC 
CAGACCAACT GGGACCGGGC AGAAGGAGAA AATGAATTCC AGATCCCTGG AATATCAGGA 
TCTGCCTCTG ACCAGTTGAA GGAAGCCAGT GGCACTGACG TGAGACAGTT GGACCCAAGC 
AGTAAAGATG TGCGTCAGAA GGGAAAACGA GGGAAAAAGA TTCTGTGTTG TGTACCAAAT 
GTTGAATTTG GATTAGAGAC CAAAGGAACT TCAAGTGAAG AAGTTAATCT GAGTCACATT 
GTACCCTGTG AGCCAGTTTC AGAAGAAAAA GCAAAGGAGT TACCTGAATG GAGTGAGAAA 
GTGGCTCACA ACATTTTGTC AGGTGCTTCC TGGGTGAGTT GGGGTTTGGT CAAAGGTGCT 
GAGTTTACTG GCAAAGCCAT CCAGAAAGGA GCGTCTAAAC TTCGAGAACG AATTCAACCA 
GAAGAAAAGC CTGTGGAAGT TAGTCCAGCT GTGACCAAGG GACTTTATAT GGCAAAGCAG 
GCGACTGGAG GAGCAGCCAA AGTCAGTCAG TTTCTGGTTG ATGGAGTTTG TACTGTGGCA 
AATTGTGTTG GAAAGGAATT AGCTCCACAC GTCAAGAAGC ACGGAAGCAA ACTTGTTCCA 
GAATCTCTTA AAAAAGACCG AAATGGAAAG TCTACCCTGG ATGGCGCTAT GGTTGTAGCA 
GCAAGTAGTG TTCAAGGATT CTCAACTGTC TGGCAAGGAT TGGAATGTGC AGCTAAATGC 
ATTGTTAACA ATGTTTCAGC AGAAACTGTA CAAACTGTCA GATACAAATA TGGGCATACT 
GCAGGAGAAG CTACACACAA CGCAGTAGAT TCTGCCATCA ATGTTGGTGT AACTGCCTAT 
AATATTGACA ACATTGGCAT CAAAGCAATG GTGAAGAAAA CTGCAAAACA AACAGGACAC 
ACACTGCTTG AGGACTATAA AATCATTGAT AATTCTAAGG GGGAAAATCC AGGAGGAGGA 
GCAAGTGCAA ACCTGAAAGG GGAGAAAGAT GAGCAGAAAG AGGGGCCAGA GAAGAACGGG 
GCAAAGAAGA AAGATAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPART ( XM_005213641.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005213698.1 Bos taurus spastic paraplegia 20 (Troyer syndrome) (SPG20), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa24680
Clone ID Related Accession (Same CDS sequence)	XM_005213641.4 , XM_005213641.3 , XM_010810718.2 , XM_010810718.3
Accession Version	XM_005213641.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2121bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	spartin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104311.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005213641.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAACAAG GACCCCAAGA TGGAGAACCT ATTGAAATTA AGATCATCAA GGAAGCATAC 
AAGAAAGCCT TTGTATTTGT TAATAAAGGA CTGAATACAG ATGAATTAGG TCAAAAGGAA 
GAAGCAAAGA ACTACTATAA GCAAGGAATA GGACACCTGC TTAGAGGAAT CAGCATTTCA 
TCAACAGATC CTGAGTACAC AGGTCCTGAG TGGGAGTCTG CCAGGCAGAT GCAACAGAAA 
ATGAAAGAAA CGCTACAGAA TGTACGTACC AGGTTGGAAA TTCTAGAGAA GGGTCTTGCC 
ACTTCTCTAC GGAATGATCT TCAGGAGGTG CCCAAGTTGT ATCCAGAATT TCCACCTAAA 
GACATGTCTG AAAAGTCACC AGAGCCTCAG AGCCTTAGTT CACTTCCTCA GCACAGTGAA 
GTAAATGGCA GCACTTCAAC TGCAAGTGCA GAATCAAGTA GTACACCTAC TACTTTGTCC 
TTGCCGTGTC AGAGTCATCC GTCCGAAGCA CCTCCTGCTT ACACTCCCCA GGCTGCTGAG 
GGTCACTACA CTGTATCCTA TGGAACAGAA TCTGGGGAGT TCTCGTCAGT TGGGGAGAAC 
TTCTATAGGA ATCACTCTCA GCCACCTCCT CTTGAGACCT TAGGGCTGGA TGCAGATGAG 
TTGATTTTGA TACCAAACGG AGTACAAATT TTCTTTGTAA ATCCCGCAGG GGAGGTTAGT 
GCACCTTCAT ATCCTGGGTA CCTTCGAATC GTGAGGTTCT TGGATAATTC TCTTGATACC 
TTTCTAAATC GTCCTCCTGG ATTTCTCCAG ATTTGTCGTC AATTCATATG GAACTTGGAT 
ATGAAGTTGC TCTGTGAAAG TGAAACTGGG AGAAATCAGC CTGTGGTTTG TGACTGGTTG 
TATCCTCTAG TTCCTGATAG GTCTCCAGTT CTTAAATGTA CTGTAGGTGC CTACATGTTT 
CCTGATACGA TGTTACAAGC ATCGGGATGC TTTGTGGGGG TCGTCTTGTC ATCTGAATTA 
CCAGAAGATG ACAGAGAACT CTTCGAGGAT CTATTAAGAC AAATGTCTGA CCTTCGGCTC 
CAGACCAACT GGGACCGGGC AGAAGGAGAA AATGAATTCC AGATCCCTGG AATATCAGGA 
TCTGCCTCTG ACCAGTTGAA GGAAGCCAGT GGCACTGACG TGAGACAGTT GGACCCAAGC 
AGTAAAGATG TGCGTCAGAA GGGAAAACGA GGGAAAAAGA TTCTGTGTTG TGTACCAAAT 
GTTGAATTTG GATTAGAGAC CAAAGGAACT TCAAGTGAAG AAGTTAATCT GAGTCACATT 
GTACCCTGTG AGCCAGTTTC AGAAGAAAAA GCAAAGGAGT TACCTGAATG GAGTGAGAAA 
GTGGCTCACA ACATTTTGTC AGGTGCTTCC TGGGTGAGTT GGGGTTTGGT CAAAGGTGCT 
GAGTTTACTG GCAAAGCCAT CCAGAAAGGA GCGTCTAAAC TTCGAGAACG AATTCAACCA 
GAAGAAAAGC CTGTGGAAGT TAGTCCAGCT GTGACCAAGG GACTTTATAT GGCAAAGCAG 
GCGACTGGAG GAGCAGCCAA AGTCAGTCAG TTTCTGGTTG ATGGAGTTTG TACTGTGGCA 
AATTGTGTTG GAAAGGAATT AGCTCCACAC GTCAAGAAGC ACGGAAGCAA ACTTGTTCCA 
GAATCTCTTA AAAAAGACCG AAATGGAAAG TCTACCCTGG ATGGCGCTAT GGTTGTAGCA 
GCAAGTAGTG TTCAAGGATT CTCAACTGTC TGGCAAGGAT TGGAATGTGC AGCTAAATGC 
ATTGTTAACA ATGTTTCAGC AGAAACTGTA CAAACTGTCA GATACAAATA TGGGCATACT 
GCAGGAGAAG CTACACACAA CGCAGTAGAT TCTGCCATCA ATGTTGGTGT AACTGCCTAT 
AATATTGACA ACATTGGCAT CAAAGCAATG GTGAAGAAAA CTGCAAAACA AACAGGACAC 
ACACTGCTTG AGGACTATAA AATCATTGAT AATTCTAAGG GGGAAAATCC AGGAGGAGGA 
GCAAGTGCAA ACCTGAAAGG GGAGAAAGAT GAGCAGAAAG AGGGGCCAGA GAAGAACGGG 
GCAAAGAAGA AAGATAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005213698.1
CDS	189..2309
Translation	MEQGPQDGEPIEIKIIKEAYKKAFVFVNKGLNTDELGQKEEAKNYYKQGIGHLLRGISISSTDPEYTGPEWESARQMQQKMKETLQNVRTRLEILEKGLATSLRNDLQEVPKLYPEFPPKDMSEKSPEPQSLSSLPQHSEVNGSTSTASAESSSTPTTLSLPCQSHPSEAPPAYTPQAAEGHYTVSYGTESGEFSSVGENFYRNHSQPPPLETLGLDADELILIPNGVQIFFVNPAGEVSAPSYPGYLRIVRFLDNSLDTFLNRPPGFLQICRQFIWNLDMKLLCESETGRNQPVVCDWLYPLVPDRSPVLKCTVGAYMFPDTMLQASGCFVGVVLSSELPEDDRELFEDLLRQMSDLRLQTNWDRAEGENEFQIPGISGSASDQLKEASGTDVRQLDPSSKDVRQKGKRGKKILCCVPNVEFGLETKGTSSEEVNLSHIVPCEPVSEEKAKELPEWSEKVAHNILSGASWVSWGLVKGAEFTGKAIQKGASKLRERIQPEEKPVEVSPAVTKGLYMAKQATGGAAKVSQFLVDGVCTVANCVGKELAPHVKKHGSKLVPESLKKDRNGKSTLDGAMVVAASSVQGFSTVWQGLECAAKCIVNNVSAETVQTVRYKYGHTAGEATHNAVDSAINVGVTAYNIDNIGIKAMVKKTAKQTGHTLLEDYKIIDNSKGENPGGGASANLKGEKDEQKEGPEKNGAKKKDK

Target ORF information:

RefSeq Version	XM_005213641.3
Organism	Bos taurus(cattle)
Definition	Bos taurus spastic paraplegia 20 (Troyer syndrome) (SPG20), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005213641.3

ORF Insert Sequence:

ATGGAACAAG GACCCCAAGA TGGAGAACCT ATTGAAATTA AGATCATCAA GGAAGCATAC 
AAGAAAGCCT TTGTATTTGT TAATAAAGGA CTGAATACAG ATGAATTAGG TCAAAAGGAA 
GAAGCAAAGA ACTACTATAA GCAAGGAATA GGACACCTGC TTAGAGGAAT CAGCATTTCA 
TCAACAGATC CTGAGTACAC AGGTCCTGAG TGGGAGTCTG CCAGGCAGAT GCAACAGAAA 
ATGAAAGAAA CGCTACAGAA TGTACGTACC AGGTTGGAAA TTCTAGAGAA GGGTCTTGCC 
ACTTCTCTAC GGAATGATCT TCAGGAGGTG CCCAAGTTGT ATCCAGAATT TCCACCTAAA 
GACATGTCTG AAAAGTCACC AGAGCCTCAG AGCCTTAGTT CACTTCCTCA GCACAGTGAA 
GTAAATGGCA GCACTTCAAC TGCAAGTGCA GAATCAAGTA GTACACCTAC TACTTTGTCC 
TTGCCGTGTC AGAGTCATCC GTCCGAAGCA CCTCCTGCTT ACACTCCCCA GGCTGCTGAG 
GGTCACTACA CTGTATCCTA TGGAACAGAA TCTGGGGAGT TCTCGTCAGT TGGGGAGAAC 
TTCTATAGGA ATCACTCTCA GCCACCTCCT CTTGAGACCT TAGGGCTGGA TGCAGATGAG 
TTGATTTTGA TACCAAACGG AGTACAAATT TTCTTTGTAA ATCCCGCAGG GGAGGTTAGT 
GCACCTTCAT ATCCTGGGTA CCTTCGAATC GTGAGGTTCT TGGATAATTC TCTTGATACC 
TTTCTAAATC GTCCTCCTGG ATTTCTCCAG ATTTGTCGTC AATTCATATG GAACTTGGAT 
ATGAAGTTGC TCTGTGAAAG TGAAACTGGG AGAAATCAGC CTGTGGTTTG TGACTGGTTG 
TATCCTCTAG TTCCTGATAG GTCTCCAGTT CTTAAATGTA CTGTAGGTGC CTACATGTTT 
CCTGATACGA TGTTACAAGC ATCGGGATGC TTTGTGGGGG TCGTCTTGTC ATCTGAATTA 
CCAGAAGATG ACAGAGAACT CTTCGAGGAT CTATTAAGAC AAATGTCTGA CCTTCGGCTC 
CAGACCAACT GGGACCGGGC AGAAGGAGAA AATGAATTCC AGATCCCTGG AATATCAGGA 
TCTGCCTCTG ACCAGTTGAA GGAAGCCAGT GGCACTGACG TGAGACAGTT GGACCCAAGC 
AGTAAAGATG TGCGTCAGAA GGGAAAACGA GGGAAAAAGA TTCTGTGTTG TGTACCAAAT 
GTTGAATTTG GATTAGAGAC CAAAGGAACT TCAAGTGAAG AAGTTAATCT GAGTCACATT 
GTACCCTGTG AGCCAGTTTC AGAAGAAAAA GCAAAGGAGT TACCTGAATG GAGTGAGAAA 
GTGGCTCACA ACATTTTGTC AGGTGCTTCC TGGGTGAGTT GGGGTTTGGT CAAAGGTGCT 
GAGTTTACTG GCAAAGCCAT CCAGAAAGGA GCGTCTAAAC TTCGAGAACG AATTCAACCA 
GAAGAAAAGC CTGTGGAAGT TAGTCCAGCT GTGACCAAGG GACTTTATAT GGCAAAGCAG 
GCGACTGGAG GAGCAGCCAA AGTCAGTCAG TTTCTGGTTG ATGGAGTTTG TACTGTGGCA 
AATTGTGTTG GAAAGGAATT AGCTCCACAC GTCAAGAAGC ACGGAAGCAA ACTTGTTCCA 
GAATCTCTTA AAAAAGACCG AAATGGAAAG TCTACCCTGG ATGGCGCTAT GGTTGTAGCA 
GCAAGTAGTG TTCAAGGATT CTCAACTGTC TGGCAAGGAT TGGAATGTGC AGCTAAATGC 
ATTGTTAACA ATGTTTCAGC AGAAACTGTA CAAACTGTCA GATACAAATA TGGGCATACT 
GCAGGAGAAG CTACACACAA CGCAGTAGAT TCTGCCATCA ATGTTGGTGT AACTGCCTAT 
AATATTGACA ACATTGGCAT CAAAGCAATG GTGAAGAAAA CTGCAAAACA AACAGGACAC 
ACACTGCTTG AGGACTATAA AATCATTGAT AATTCTAAGG GGGAAAATCC AGGAGGAGGA 
GCAAGTGCAA ACCTGAAAGG GGAGAAAGAT GAGCAGAAAG AGGGGCCAGA GAAGAACGGG 
GCAAAGAAGA AAGATAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPART ( XM_010810718.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010809020.1 Bos taurus spastic paraplegia 20 (Troyer syndrome) (SPG20), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa24680
Clone ID Related Accession (Same CDS sequence)	XM_005213641.4 , XM_005213641.3 , XM_010810718.2 , XM_010810718.3
Accession Version	XM_010810718.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2121bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	spartin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104311.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010810718.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAACAAG GACCCCAAGA TGGAGAACCT ATTGAAATTA AGATCATCAA GGAAGCATAC 
AAGAAAGCCT TTGTATTTGT TAATAAAGGA CTGAATACAG ATGAATTAGG TCAAAAGGAA 
GAAGCAAAGA ACTACTATAA GCAAGGAATA GGACACCTGC TTAGAGGAAT CAGCATTTCA 
TCAACAGATC CTGAGTACAC AGGTCCTGAG TGGGAGTCTG CCAGGCAGAT GCAACAGAAA 
ATGAAAGAAA CGCTACAGAA TGTACGTACC AGGTTGGAAA TTCTAGAGAA GGGTCTTGCC 
ACTTCTCTAC GGAATGATCT TCAGGAGGTG CCCAAGTTGT ATCCAGAATT TCCACCTAAA 
GACATGTCTG AAAAGTCACC AGAGCCTCAG AGCCTTAGTT CACTTCCTCA GCACAGTGAA 
GTAAATGGCA GCACTTCAAC TGCAAGTGCA GAATCAAGTA GTACACCTAC TACTTTGTCC 
TTGCCGTGTC AGAGTCATCC GTCCGAAGCA CCTCCTGCTT ACACTCCCCA GGCTGCTGAG 
GGTCACTACA CTGTATCCTA TGGAACAGAA TCTGGGGAGT TCTCGTCAGT TGGGGAGAAC 
TTCTATAGGA ATCACTCTCA GCCACCTCCT CTTGAGACCT TAGGGCTGGA TGCAGATGAG 
TTGATTTTGA TACCAAACGG AGTACAAATT TTCTTTGTAA ATCCCGCAGG GGAGGTTAGT 
GCACCTTCAT ATCCTGGGTA CCTTCGAATC GTGAGGTTCT TGGATAATTC TCTTGATACC 
TTTCTAAATC GTCCTCCTGG ATTTCTCCAG ATTTGTCGTC AATTCATATG GAACTTGGAT 
ATGAAGTTGC TCTGTGAAAG TGAAACTGGG AGAAATCAGC CTGTGGTTTG TGACTGGTTG 
TATCCTCTAG TTCCTGATAG GTCTCCAGTT CTTAAATGTA CTGTAGGTGC CTACATGTTT 
CCTGATACGA TGTTACAAGC ATCGGGATGC TTTGTGGGGG TCGTCTTGTC ATCTGAATTA 
CCAGAAGATG ACAGAGAACT CTTCGAGGAT CTATTAAGAC AAATGTCTGA CCTTCGGCTC 
CAGACCAACT GGGACCGGGC AGAAGGAGAA AATGAATTCC AGATCCCTGG AATATCAGGA 
TCTGCCTCTG ACCAGTTGAA GGAAGCCAGT GGCACTGACG TGAGACAGTT GGACCCAAGC 
AGTAAAGATG TGCGTCAGAA GGGAAAACGA GGGAAAAAGA TTCTGTGTTG TGTACCAAAT 
GTTGAATTTG GATTAGAGAC CAAAGGAACT TCAAGTGAAG AAGTTAATCT GAGTCACATT 
GTACCCTGTG AGCCAGTTTC AGAAGAAAAA GCAAAGGAGT TACCTGAATG GAGTGAGAAA 
GTGGCTCACA ACATTTTGTC AGGTGCTTCC TGGGTGAGTT GGGGTTTGGT CAAAGGTGCT 
GAGTTTACTG GCAAAGCCAT CCAGAAAGGA GCGTCTAAAC TTCGAGAACG AATTCAACCA 
GAAGAAAAGC CTGTGGAAGT TAGTCCAGCT GTGACCAAGG GACTTTATAT GGCAAAGCAG 
GCGACTGGAG GAGCAGCCAA AGTCAGTCAG TTTCTGGTTG ATGGAGTTTG TACTGTGGCA 
AATTGTGTTG GAAAGGAATT AGCTCCACAC GTCAAGAAGC ACGGAAGCAA ACTTGTTCCA 
GAATCTCTTA AAAAAGACCG AAATGGAAAG TCTACCCTGG ATGGCGCTAT GGTTGTAGCA 
GCAAGTAGTG TTCAAGGATT CTCAACTGTC TGGCAAGGAT TGGAATGTGC AGCTAAATGC 
ATTGTTAACA ATGTTTCAGC AGAAACTGTA CAAACTGTCA GATACAAATA TGGGCATACT 
GCAGGAGAAG CTACACACAA CGCAGTAGAT TCTGCCATCA ATGTTGGTGT AACTGCCTAT 
AATATTGACA ACATTGGCAT CAAAGCAATG GTGAAGAAAA CTGCAAAACA AACAGGACAC 
ACACTGCTTG AGGACTATAA AATCATTGAT AATTCTAAGG GGGAAAATCC AGGAGGAGGA 
GCAAGTGCAA ACCTGAAAGG GGAGAAAGAT GAGCAGAAAG AGGGGCCAGA GAAGAACGGG 
GCAAAGAAGA AAGATAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010809020.1
CDS	142..2262
Translation	MEQGPQDGEPIEIKIIKEAYKKAFVFVNKGLNTDELGQKEEAKNYYKQGIGHLLRGISISSTDPEYTGPEWESARQMQQKMKETLQNVRTRLEILEKGLATSLRNDLQEVPKLYPEFPPKDMSEKSPEPQSLSSLPQHSEVNGSTSTASAESSSTPTTLSLPCQSHPSEAPPAYTPQAAEGHYTVSYGTESGEFSSVGENFYRNHSQPPPLETLGLDADELILIPNGVQIFFVNPAGEVSAPSYPGYLRIVRFLDNSLDTFLNRPPGFLQICRQFIWNLDMKLLCESETGRNQPVVCDWLYPLVPDRSPVLKCTVGAYMFPDTMLQASGCFVGVVLSSELPEDDRELFEDLLRQMSDLRLQTNWDRAEGENEFQIPGISGSASDQLKEASGTDVRQLDPSSKDVRQKGKRGKKILCCVPNVEFGLETKGTSSEEVNLSHIVPCEPVSEEKAKELPEWSEKVAHNILSGASWVSWGLVKGAEFTGKAIQKGASKLRERIQPEEKPVEVSPAVTKGLYMAKQATGGAAKVSQFLVDGVCTVANCVGKELAPHVKKHGSKLVPESLKKDRNGKSTLDGAMVVAASSVQGFSTVWQGLECAAKCIVNNVSAETVQTVRYKYGHTAGEATHNAVDSAINVGVTAYNIDNIGIKAMVKKTAKQTGHTLLEDYKIIDNSKGENPGGGASANLKGEKDEQKEGPEKNGAKKKDK

Target ORF information:

RefSeq Version	XM_010810718.2
Organism	Bos taurus(cattle)
Definition	Bos taurus spastic paraplegia 20 (Troyer syndrome) (SPG20), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010810718.2

ORF Insert Sequence:

ATGGAACAAG GACCCCAAGA TGGAGAACCT ATTGAAATTA AGATCATCAA GGAAGCATAC 
AAGAAAGCCT TTGTATTTGT TAATAAAGGA CTGAATACAG ATGAATTAGG TCAAAAGGAA 
GAAGCAAAGA ACTACTATAA GCAAGGAATA GGACACCTGC TTAGAGGAAT CAGCATTTCA 
TCAACAGATC CTGAGTACAC AGGTCCTGAG TGGGAGTCTG CCAGGCAGAT GCAACAGAAA 
ATGAAAGAAA CGCTACAGAA TGTACGTACC AGGTTGGAAA TTCTAGAGAA GGGTCTTGCC 
ACTTCTCTAC GGAATGATCT TCAGGAGGTG CCCAAGTTGT ATCCAGAATT TCCACCTAAA 
GACATGTCTG AAAAGTCACC AGAGCCTCAG AGCCTTAGTT CACTTCCTCA GCACAGTGAA 
GTAAATGGCA GCACTTCAAC TGCAAGTGCA GAATCAAGTA GTACACCTAC TACTTTGTCC 
TTGCCGTGTC AGAGTCATCC GTCCGAAGCA CCTCCTGCTT ACACTCCCCA GGCTGCTGAG 
GGTCACTACA CTGTATCCTA TGGAACAGAA TCTGGGGAGT TCTCGTCAGT TGGGGAGAAC 
TTCTATAGGA ATCACTCTCA GCCACCTCCT CTTGAGACCT TAGGGCTGGA TGCAGATGAG 
TTGATTTTGA TACCAAACGG AGTACAAATT TTCTTTGTAA ATCCCGCAGG GGAGGTTAGT 
GCACCTTCAT ATCCTGGGTA CCTTCGAATC GTGAGGTTCT TGGATAATTC TCTTGATACC 
TTTCTAAATC GTCCTCCTGG ATTTCTCCAG ATTTGTCGTC AATTCATATG GAACTTGGAT 
ATGAAGTTGC TCTGTGAAAG TGAAACTGGG AGAAATCAGC CTGTGGTTTG TGACTGGTTG 
TATCCTCTAG TTCCTGATAG GTCTCCAGTT CTTAAATGTA CTGTAGGTGC CTACATGTTT 
CCTGATACGA TGTTACAAGC ATCGGGATGC TTTGTGGGGG TCGTCTTGTC ATCTGAATTA 
CCAGAAGATG ACAGAGAACT CTTCGAGGAT CTATTAAGAC AAATGTCTGA CCTTCGGCTC 
CAGACCAACT GGGACCGGGC AGAAGGAGAA AATGAATTCC AGATCCCTGG AATATCAGGA 
TCTGCCTCTG ACCAGTTGAA GGAAGCCAGT GGCACTGACG TGAGACAGTT GGACCCAAGC 
AGTAAAGATG TGCGTCAGAA GGGAAAACGA GGGAAAAAGA TTCTGTGTTG TGTACCAAAT 
GTTGAATTTG GATTAGAGAC CAAAGGAACT TCAAGTGAAG AAGTTAATCT GAGTCACATT 
GTACCCTGTG AGCCAGTTTC AGAAGAAAAA GCAAAGGAGT TACCTGAATG GAGTGAGAAA 
GTGGCTCACA ACATTTTGTC AGGTGCTTCC TGGGTGAGTT GGGGTTTGGT CAAAGGTGCT 
GAGTTTACTG GCAAAGCCAT CCAGAAAGGA GCGTCTAAAC TTCGAGAACG AATTCAACCA 
GAAGAAAAGC CTGTGGAAGT TAGTCCAGCT GTGACCAAGG GACTTTATAT GGCAAAGCAG 
GCGACTGGAG GAGCAGCCAA AGTCAGTCAG TTTCTGGTTG ATGGAGTTTG TACTGTGGCA 
AATTGTGTTG GAAAGGAATT AGCTCCACAC GTCAAGAAGC ACGGAAGCAA ACTTGTTCCA 
GAATCTCTTA AAAAAGACCG AAATGGAAAG TCTACCCTGG ATGGCGCTAT GGTTGTAGCA 
GCAAGTAGTG TTCAAGGATT CTCAACTGTC TGGCAAGGAT TGGAATGTGC AGCTAAATGC 
ATTGTTAACA ATGTTTCAGC AGAAACTGTA CAAACTGTCA GATACAAATA TGGGCATACT 
GCAGGAGAAG CTACACACAA CGCAGTAGAT TCTGCCATCA ATGTTGGTGT AACTGCCTAT 
AATATTGACA ACATTGGCAT CAAAGCAATG GTGAAGAAAA CTGCAAAACA AACAGGACAC 
ACACTGCTTG AGGACTATAA AATCATTGAT AATTCTAAGG GGGAAAATCC AGGAGGAGGA 
GCAAGTGCAA ACCTGAAAGG GGAGAAAGAT GAGCAGAAAG AGGGGCCAGA GAAGAACGGG 
GCAAAGAAGA AAGATAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPART ( XM_010810718.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010809020.1 Bos taurus spartin (SPART), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa24680
Clone ID Related Accession (Same CDS sequence)	XM_005213641.4 , XM_005213641.3 , XM_010810718.2 , XM_010810718.3
Accession Version	XM_010810718.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2121bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	spartin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037339.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010810718.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAACAAG GACCCCAAGA TGGAGAACCT ATTGAAATTA AGATCATCAA GGAAGCATAC 
AAGAAAGCCT TTGTATTTGT TAATAAAGGA CTGAATACAG ATGAATTAGG TCAAAAGGAA 
GAAGCAAAGA ACTACTATAA GCAAGGAATA GGACACCTGC TTAGAGGAAT CAGCATTTCA 
TCAACAGATC CTGAGTACAC AGGTCCTGAG TGGGAGTCTG CCAGGCAGAT GCAACAGAAA 
ATGAAAGAAA CGCTACAGAA TGTACGTACC AGGTTGGAAA TTCTAGAGAA GGGTCTTGCC 
ACTTCTCTAC GGAATGATCT TCAGGAGGTG CCCAAGTTGT ATCCAGAATT TCCACCTAAA 
GACATGTCTG AAAAGTCACC AGAGCCTCAG AGCCTTAGTT CACTTCCTCA GCACAGTGAA 
GTAAATGGCA GCACTTCAAC TGCAAGTGCA GAATCAAGTA GTACACCTAC TACTTTGTCC 
TTGCCGTGTC AGAGTCATCC GTCCGAAGCA CCTCCTGCTT ACACTCCCCA GGCTGCTGAG 
GGTCACTACA CTGTATCCTA TGGAACAGAA TCTGGGGAGT TCTCGTCAGT TGGGGAGAAC 
TTCTATAGGA ATCACTCTCA GCCACCTCCT CTTGAGACCT TAGGGCTGGA TGCAGATGAG 
TTGATTTTGA TACCAAACGG AGTACAAATT TTCTTTGTAA ATCCCGCAGG GGAGGTTAGT 
GCACCTTCAT ATCCTGGGTA CCTTCGAATC GTGAGGTTCT TGGATAATTC TCTTGATACC 
TTTCTAAATC GTCCTCCTGG ATTTCTCCAG ATTTGTCGTC AATTCATATG GAACTTGGAT 
ATGAAGTTGC TCTGTGAAAG TGAAACTGGG AGAAATCAGC CTGTGGTTTG TGACTGGTTG 
TATCCTCTAG TTCCTGATAG GTCTCCAGTT CTTAAATGTA CTGTAGGTGC CTACATGTTT 
CCTGATACGA TGTTACAAGC ATCGGGATGC TTTGTGGGGG TCGTCTTGTC ATCTGAATTA 
CCAGAAGATG ACAGAGAACT CTTCGAGGAT CTATTAAGAC AAATGTCTGA CCTTCGGCTC 
CAGACCAACT GGGACCGGGC AGAAGGAGAA AATGAATTCC AGATCCCTGG AATATCAGGA 
TCTGCCTCTG ACCAGTTGAA GGAAGCCAGT GGCACTGACG TGAGACAGTT GGACCCAAGC 
AGTAAAGATG TGCGTCAGAA GGGAAAACGA GGGAAAAAGA TTCTGTGTTG TGTACCAAAT 
GTTGAATTTG GATTAGAGAC CAAAGGAACT TCAAGTGAAG AAGTTAATCT GAGTCACATT 
GTACCCTGTG AGCCAGTTTC AGAAGAAAAA GCAAAGGAGT TACCTGAATG GAGTGAGAAA 
GTGGCTCACA ACATTTTGTC AGGTGCTTCC TGGGTGAGTT GGGGTTTGGT CAAAGGTGCT 
GAGTTTACTG GCAAAGCCAT CCAGAAAGGA GCGTCTAAAC TTCGAGAACG AATTCAACCA 
GAAGAAAAGC CTGTGGAAGT TAGTCCAGCT GTGACCAAGG GACTTTATAT GGCAAAGCAG 
GCGACTGGAG GAGCAGCCAA AGTCAGTCAG TTTCTGGTTG ATGGAGTTTG TACTGTGGCA 
AATTGTGTTG GAAAGGAATT AGCTCCACAC GTCAAGAAGC ACGGAAGCAA ACTTGTTCCA 
GAATCTCTTA AAAAAGACCG AAATGGAAAG TCTACCCTGG ATGGCGCTAT GGTTGTAGCA 
GCAAGTAGTG TTCAAGGATT CTCAACTGTC TGGCAAGGAT TGGAATGTGC AGCTAAATGC 
ATTGTTAACA ATGTTTCAGC AGAAACTGTA CAAACTGTCA GATACAAATA TGGGCATACT 
GCAGGAGAAG CTACACACAA CGCAGTAGAT TCTGCCATCA ATGTTGGTGT AACTGCCTAT 
AATATTGACA ACATTGGCAT CAAAGCAATG GTGAAGAAAA CTGCAAAACA AACAGGACAC 
ACACTGCTTG AGGACTATAA AATCATTGAT AATTCTAAGG GGGAAAATCC AGGAGGAGGA 
GCAAGTGCAA ACCTGAAAGG GGAGAAAGAT GAGCAGAAAG AGGGGCCAGA GAAGAACGGG 
GCAAAGAAGA AAGATAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010809020.1
CDS	156..2276
Translation	MEQGPQDGEPIEIKIIKEAYKKAFVFVNKGLNTDELGQKEEAKNYYKQGIGHLLRGISISSTDPEYTGPEWESARQMQQKMKETLQNVRTRLEILEKGLATSLRNDLQEVPKLYPEFPPKDMSEKSPEPQSLSSLPQHSEVNGSTSTASAESSSTPTTLSLPCQSHPSEAPPAYTPQAAEGHYTVSYGTESGEFSSVGENFYRNHSQPPPLETLGLDADELILIPNGVQIFFVNPAGEVSAPSYPGYLRIVRFLDNSLDTFLNRPPGFLQICRQFIWNLDMKLLCESETGRNQPVVCDWLYPLVPDRSPVLKCTVGAYMFPDTMLQASGCFVGVVLSSELPEDDRELFEDLLRQMSDLRLQTNWDRAEGENEFQIPGISGSASDQLKEASGTDVRQLDPSSKDVRQKGKRGKKILCCVPNVEFGLETKGTSSEEVNLSHIVPCEPVSEEKAKELPEWSEKVAHNILSGASWVSWGLVKGAEFTGKAIQKGASKLRERIQPEEKPVEVSPAVTKGLYMAKQATGGAAKVSQFLVDGVCTVANCVGKELAPHVKKHGSKLVPESLKKDRNGKSTLDGAMVVAASSVQGFSTVWQGLECAAKCIVNNVSAETVQTVRYKYGHTAGEATHNAVDSAINVGVTAYNIDNIGIKAMVKKTAKQTGHTLLEDYKIIDNSKGENPGGGASANLKGEKDEQKEGPEKNGAKKKDK

Target ORF information:

RefSeq Version	XM_010810718.3
Organism	Bos taurus(cattle)
Definition	Bos taurus spartin (SPART), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010810718.3

ORF Insert Sequence:

ATGGAACAAG GACCCCAAGA TGGAGAACCT ATTGAAATTA AGATCATCAA GGAAGCATAC 
AAGAAAGCCT TTGTATTTGT TAATAAAGGA CTGAATACAG ATGAATTAGG TCAAAAGGAA 
GAAGCAAAGA ACTACTATAA GCAAGGAATA GGACACCTGC TTAGAGGAAT CAGCATTTCA 
TCAACAGATC CTGAGTACAC AGGTCCTGAG TGGGAGTCTG CCAGGCAGAT GCAACAGAAA 
ATGAAAGAAA CGCTACAGAA TGTACGTACC AGGTTGGAAA TTCTAGAGAA GGGTCTTGCC 
ACTTCTCTAC GGAATGATCT TCAGGAGGTG CCCAAGTTGT ATCCAGAATT TCCACCTAAA 
GACATGTCTG AAAAGTCACC AGAGCCTCAG AGCCTTAGTT CACTTCCTCA GCACAGTGAA 
GTAAATGGCA GCACTTCAAC TGCAAGTGCA GAATCAAGTA GTACACCTAC TACTTTGTCC 
TTGCCGTGTC AGAGTCATCC GTCCGAAGCA CCTCCTGCTT ACACTCCCCA GGCTGCTGAG 
GGTCACTACA CTGTATCCTA TGGAACAGAA TCTGGGGAGT TCTCGTCAGT TGGGGAGAAC 
TTCTATAGGA ATCACTCTCA GCCACCTCCT CTTGAGACCT TAGGGCTGGA TGCAGATGAG 
TTGATTTTGA TACCAAACGG AGTACAAATT TTCTTTGTAA ATCCCGCAGG GGAGGTTAGT 
GCACCTTCAT ATCCTGGGTA CCTTCGAATC GTGAGGTTCT TGGATAATTC TCTTGATACC 
TTTCTAAATC GTCCTCCTGG ATTTCTCCAG ATTTGTCGTC AATTCATATG GAACTTGGAT 
ATGAAGTTGC TCTGTGAAAG TGAAACTGGG AGAAATCAGC CTGTGGTTTG TGACTGGTTG 
TATCCTCTAG TTCCTGATAG GTCTCCAGTT CTTAAATGTA CTGTAGGTGC CTACATGTTT 
CCTGATACGA TGTTACAAGC ATCGGGATGC TTTGTGGGGG TCGTCTTGTC ATCTGAATTA 
CCAGAAGATG ACAGAGAACT CTTCGAGGAT CTATTAAGAC AAATGTCTGA CCTTCGGCTC 
CAGACCAACT GGGACCGGGC AGAAGGAGAA AATGAATTCC AGATCCCTGG AATATCAGGA 
TCTGCCTCTG ACCAGTTGAA GGAAGCCAGT GGCACTGACG TGAGACAGTT GGACCCAAGC 
AGTAAAGATG TGCGTCAGAA GGGAAAACGA GGGAAAAAGA TTCTGTGTTG TGTACCAAAT 
GTTGAATTTG GATTAGAGAC CAAAGGAACT TCAAGTGAAG AAGTTAATCT GAGTCACATT 
GTACCCTGTG AGCCAGTTTC AGAAGAAAAA GCAAAGGAGT TACCTGAATG GAGTGAGAAA 
GTGGCTCACA ACATTTTGTC AGGTGCTTCC TGGGTGAGTT GGGGTTTGGT CAAAGGTGCT 
GAGTTTACTG GCAAAGCCAT CCAGAAAGGA GCGTCTAAAC TTCGAGAACG AATTCAACCA 
GAAGAAAAGC CTGTGGAAGT TAGTCCAGCT GTGACCAAGG GACTTTATAT GGCAAAGCAG 
GCGACTGGAG GAGCAGCCAA AGTCAGTCAG TTTCTGGTTG ATGGAGTTTG TACTGTGGCA 
AATTGTGTTG GAAAGGAATT AGCTCCACAC GTCAAGAAGC ACGGAAGCAA ACTTGTTCCA 
GAATCTCTTA AAAAAGACCG AAATGGAAAG TCTACCCTGG ATGGCGCTAT GGTTGTAGCA 
GCAAGTAGTG TTCAAGGATT CTCAACTGTC TGGCAAGGAT TGGAATGTGC AGCTAAATGC 
ATTGTTAACA ATGTTTCAGC AGAAACTGTA CAAACTGTCA GATACAAATA TGGGCATACT 
GCAGGAGAAG CTACACACAA CGCAGTAGAT TCTGCCATCA ATGTTGGTGT AACTGCCTAT 
AATATTGACA ACATTGGCAT CAAAGCAATG GTGAAGAAAA CTGCAAAACA AACAGGACAC 
ACACTGCTTG AGGACTATAA AATCATTGAT AATTCTAAGG GGGAAAATCC AGGAGGAGGA 
GCAAGTGCAA ACCTGAAAGG GGAGAAAGAT GAGCAGAAAG AGGGGCCAGA GAAGAACGGG 
GCAAAGAAGA AAGATAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPART ( XM_005213642.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005213699.1 Bos taurus spartin (SPART), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa24681
Clone ID Related Accession (Same CDS sequence)	XM_005213642.3
Accession Version	XM_005213642.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2082bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	spartin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037339.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005213642.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAACAAG GACCCCAAGA TGGAGAACCT ATTGAAATTA AGATCATCAA GGAAGCATAC 
AAGAAAGCCT TTGTATTTGT TAATAAAGGA CTGAATACAG ATGAATTAGG TCAAAAGGAA 
GAAGCAAAGA ACTACTATAA GCAAGGAATA GGACACCTGC TTAGAGGAAT CAGCATTTCA 
TCAACAGATC CTGAGTACAC AGGTCCTGAG TGGGAGTCTG CCAGGCAGAT GCAACAGAAA 
ATGAAAGAAA CGCTACAGAA TGTACGTACC AGGTTGGAAA TTCTAGAGAA GGGTCTTGCC 
ACTTCTCTAC GGAATGATCT TCAGGAGGTG CCCAAGTTGT ATCCAGAATT TCCACCTAAA 
GACATGTCTG AAAAGTCACC AGAGCCTCAG AGCCTTAGTT CACTTCCTCA GCACAGTGAA 
GTAAATGGCA GCACTTCAAC TGCAAGTGCA GAATCAAGTA GTACACCTAC TACTTTGTCC 
TTGCCGTGTC AGAGTCATCC GTCCGAAGCA CCTCCTGCTT ACACTCCCCA GGCTGCTGAG 
GGTCACTACA CTGTATCCTA TGGAACAGAA TCTGGGGAGT TCTCGTCAGT TGGGGAGAAC 
TTCTATAGGA ATCACTCTCA GCCACCTCCT CTTGAGACCT TAGGGCTGGA TGCAGATGAG 
TTGATTTTGA TACCAAACGG AGTACAAATT TTCTTTGTAA ATCCCGCAGG GGAGGTTAGT 
GCACCTTCAT ATCCTGGGTA CCTTCGAATC GTGAGGTTCT TGGATAATTC TCTTGATACC 
TTTCTAAATC GTCCTCCTGG ATTTCTCCAG ATTTGTCGTC AATTCATATG GAACTTGGAT 
ATGAAGTTGC TCTGTGAAAG TGAAACTGGG AGAAATCAGC CTGTGGTTTG TGACTGGTTG 
TATCCTCTAG TTCCTGATAG GTCTCCAGTT CTTAAATGTA CTGTAGGTGC CTACATGTTT 
CCTGATACGA TGTTACAAGC ATCGGGATGC TTTGTGGGGG TCGTCTTGTC ATCTGAATTA 
CCAGAAGATG ACAGAGAACT CTTCGAGGAT CTATTAAGAC AAATGTCTGA CCTTCGGCTC 
CAGACCAACT GGGACCGGGC AGAAGGAGAA AATGAATTCC AGATCCCTGG AATATCAGGA 
TCTGCCTCTG ACCAGTTGAA GGAAGCCAGT GGCACTGACG TGAGACAGTT GGACCCAAGC 
AGTAAAGATG TGCGTCAGAA GGGAAAACGA GGGAAAAAGA CCAAAGGAAC TTCAAGTGAA 
GAAGTTAATC TGAGTCACAT TGTACCCTGT GAGCCAGTTT CAGAAGAAAA AGCAAAGGAG 
TTACCTGAAT GGAGTGAGAA AGTGGCTCAC AACATTTTGT CAGGTGCTTC CTGGGTGAGT 
TGGGGTTTGG TCAAAGGTGC TGAGTTTACT GGCAAAGCCA TCCAGAAAGG AGCGTCTAAA 
CTTCGAGAAC GAATTCAACC AGAAGAAAAG CCTGTGGAAG TTAGTCCAGC TGTGACCAAG 
GGACTTTATA TGGCAAAGCA GGCGACTGGA GGAGCAGCCA AAGTCAGTCA GTTTCTGGTT 
GATGGAGTTT GTACTGTGGC AAATTGTGTT GGAAAGGAAT TAGCTCCACA CGTCAAGAAG 
CACGGAAGCA AACTTGTTCC AGAATCTCTT AAAAAAGACC GAAATGGAAA GTCTACCCTG 
GATGGCGCTA TGGTTGTAGC AGCAAGTAGT GTTCAAGGAT TCTCAACTGT CTGGCAAGGA 
TTGGAATGTG CAGCTAAATG CATTGTTAAC AATGTTTCAG CAGAAACTGT ACAAACTGTC 
AGATACAAAT ATGGGCATAC TGCAGGAGAA GCTACACACA ACGCAGTAGA TTCTGCCATC 
AATGTTGGTG TAACTGCCTA TAATATTGAC AACATTGGCA TCAAAGCAAT GGTGAAGAAA 
ACTGCAAAAC AAACAGGACA CACACTGCTT GAGGACTATA AAATCATTGA TAATTCTAAG 
GGGGAAAATC CAGGAGGAGG AGCAAGTGCA AACCTGAAAG GGGAGAAAGA TGAGCAGAAA 
GAGGGGCCAG AGAAGAACGG GGCAAAGAAG AAAGATAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005213699.1
CDS	189..2270
Translation	MEQGPQDGEPIEIKIIKEAYKKAFVFVNKGLNTDELGQKEEAKNYYKQGIGHLLRGISISSTDPEYTGPEWESARQMQQKMKETLQNVRTRLEILEKGLATSLRNDLQEVPKLYPEFPPKDMSEKSPEPQSLSSLPQHSEVNGSTSTASAESSSTPTTLSLPCQSHPSEAPPAYTPQAAEGHYTVSYGTESGEFSSVGENFYRNHSQPPPLETLGLDADELILIPNGVQIFFVNPAGEVSAPSYPGYLRIVRFLDNSLDTFLNRPPGFLQICRQFIWNLDMKLLCESETGRNQPVVCDWLYPLVPDRSPVLKCTVGAYMFPDTMLQASGCFVGVVLSSELPEDDRELFEDLLRQMSDLRLQTNWDRAEGENEFQIPGISGSASDQLKEASGTDVRQLDPSSKDVRQKGKRGKKTKGTSSEEVNLSHIVPCEPVSEEKAKELPEWSEKVAHNILSGASWVSWGLVKGAEFTGKAIQKGASKLRERIQPEEKPVEVSPAVTKGLYMAKQATGGAAKVSQFLVDGVCTVANCVGKELAPHVKKHGSKLVPESLKKDRNGKSTLDGAMVVAASSVQGFSTVWQGLECAAKCIVNNVSAETVQTVRYKYGHTAGEATHNAVDSAINVGVTAYNIDNIGIKAMVKKTAKQTGHTLLEDYKIIDNSKGENPGGGASANLKGEKDEQKEGPEKNGAKKKDK

Target ORF information:

RefSeq Version	XM_005213642.3
Organism	Bos taurus(cattle)
Definition	Bos taurus spartin (SPART), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005213642.3

ORF Insert Sequence:

ATGGAACAAG GACCCCAAGA TGGAGAACCT ATTGAAATTA AGATCATCAA GGAAGCATAC 
AAGAAAGCCT TTGTATTTGT TAATAAAGGA CTGAATACAG ATGAATTAGG TCAAAAGGAA 
GAAGCAAAGA ACTACTATAA GCAAGGAATA GGACACCTGC TTAGAGGAAT CAGCATTTCA 
TCAACAGATC CTGAGTACAC AGGTCCTGAG TGGGAGTCTG CCAGGCAGAT GCAACAGAAA 
ATGAAAGAAA CGCTACAGAA TGTACGTACC AGGTTGGAAA TTCTAGAGAA GGGTCTTGCC 
ACTTCTCTAC GGAATGATCT TCAGGAGGTG CCCAAGTTGT ATCCAGAATT TCCACCTAAA 
GACATGTCTG AAAAGTCACC AGAGCCTCAG AGCCTTAGTT CACTTCCTCA GCACAGTGAA 
GTAAATGGCA GCACTTCAAC TGCAAGTGCA GAATCAAGTA GTACACCTAC TACTTTGTCC 
TTGCCGTGTC AGAGTCATCC GTCCGAAGCA CCTCCTGCTT ACACTCCCCA GGCTGCTGAG 
GGTCACTACA CTGTATCCTA TGGAACAGAA TCTGGGGAGT TCTCGTCAGT TGGGGAGAAC 
TTCTATAGGA ATCACTCTCA GCCACCTCCT CTTGAGACCT TAGGGCTGGA TGCAGATGAG 
TTGATTTTGA TACCAAACGG AGTACAAATT TTCTTTGTAA ATCCCGCAGG GGAGGTTAGT 
GCACCTTCAT ATCCTGGGTA CCTTCGAATC GTGAGGTTCT TGGATAATTC TCTTGATACC 
TTTCTAAATC GTCCTCCTGG ATTTCTCCAG ATTTGTCGTC AATTCATATG GAACTTGGAT 
ATGAAGTTGC TCTGTGAAAG TGAAACTGGG AGAAATCAGC CTGTGGTTTG TGACTGGTTG 
TATCCTCTAG TTCCTGATAG GTCTCCAGTT CTTAAATGTA CTGTAGGTGC CTACATGTTT 
CCTGATACGA TGTTACAAGC ATCGGGATGC TTTGTGGGGG TCGTCTTGTC ATCTGAATTA 
CCAGAAGATG ACAGAGAACT CTTCGAGGAT CTATTAAGAC AAATGTCTGA CCTTCGGCTC 
CAGACCAACT GGGACCGGGC AGAAGGAGAA AATGAATTCC AGATCCCTGG AATATCAGGA 
TCTGCCTCTG ACCAGTTGAA GGAAGCCAGT GGCACTGACG TGAGACAGTT GGACCCAAGC 
AGTAAAGATG TGCGTCAGAA GGGAAAACGA GGGAAAAAGA CCAAAGGAAC TTCAAGTGAA 
GAAGTTAATC TGAGTCACAT TGTACCCTGT GAGCCAGTTT CAGAAGAAAA AGCAAAGGAG 
TTACCTGAAT GGAGTGAGAA AGTGGCTCAC AACATTTTGT CAGGTGCTTC CTGGGTGAGT 
TGGGGTTTGG TCAAAGGTGC TGAGTTTACT GGCAAAGCCA TCCAGAAAGG AGCGTCTAAA 
CTTCGAGAAC GAATTCAACC AGAAGAAAAG CCTGTGGAAG TTAGTCCAGC TGTGACCAAG 
GGACTTTATA TGGCAAAGCA GGCGACTGGA GGAGCAGCCA AAGTCAGTCA GTTTCTGGTT 
GATGGAGTTT GTACTGTGGC AAATTGTGTT GGAAAGGAAT TAGCTCCACA CGTCAAGAAG 
CACGGAAGCA AACTTGTTCC AGAATCTCTT AAAAAAGACC GAAATGGAAA GTCTACCCTG 
GATGGCGCTA TGGTTGTAGC AGCAAGTAGT GTTCAAGGAT TCTCAACTGT CTGGCAAGGA 
TTGGAATGTG CAGCTAAATG CATTGTTAAC AATGTTTCAG CAGAAACTGT ACAAACTGTC 
AGATACAAAT ATGGGCATAC TGCAGGAGAA GCTACACACA ACGCAGTAGA TTCTGCCATC 
AATGTTGGTG TAACTGCCTA TAATATTGAC AACATTGGCA TCAAAGCAAT GGTGAAGAAA 
ACTGCAAAAC AAACAGGACA CACACTGCTT GAGGACTATA AAATCATTGA TAATTCTAAG 
GGGGAAAATC CAGGAGGAGG AGCAAGTGCA AACCTGAAAG GGGAGAAAGA TGAGCAGAAA 
GAGGGGCCAG AGAAGAACGG GGCAAAGAAG AAAGATAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPART ( XM_005213643.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005213700.1 Bos taurus spastic paraplegia 20 (Troyer syndrome) (SPG20), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBa24682
Clone ID Related Accession (Same CDS sequence)	XM_005213643.3 , XM_005213643.4
Accession Version	XM_005213643.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2046bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	spartin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104311.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005213643.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAACAAG GACCCCAAGA TGGAGAACCT ATTGAAATTA AGATCATCAA GGAAGCATAC 
AAGAAAGCCT TTGTATTTGT TAATAAAGGA CTGAATACAG ATGAATTAGG TCAAAAGGAA 
GAAGCAAAGA ACTACTATAA GCAAGGAATA GGACACCTGC TTAGAGGAAT CAGCATTTCA 
TCAACAGATC CTGAGTACAC AGGTCCTGAG TGGGAGTCTG CCAGGCAGAT GCAACAGAAA 
ATGAAAGAAA CGCTACAGAA TGTACGTACC AGGTTGGAAA TTCTAGAGAA GGGTCTTGCC 
ACTTCTCTAC GGAATGATCT TCAGGAGGTG CCCAAGTTGT ATCCAGAATT TCCACCTAAA 
GACATGTCTG AAAAGTCACC AGAGCCTCAG AGCCTTAGTT CACTTCCTCA GCACAGTGAA 
GTAAATGGCA GCACTTCAAC TGCAAGTGCA GAATCAAGTA GTACACCTAC TACTTTGTCC 
TTGCCGTGTC AGAGTCATCC GTCCGAAGCA CCTCCTGCTT ACACTCCCCA GGCTGCTGAG 
GGTCACTACA CTGTATCCTA TGGAACAGAA TCTGGGGAGT TCTCGTCAGT TGGGGAGAAC 
TTCTATAGGA ATCACTCTCA GCCACCTCCT CTTGAGACCT TAGGGCTGGA TGCAGATGAG 
TTGATTTTGA TACCAAACGG AGTACAAATT TTCTTTGTAA ATCCCGCAGG GGAGGTTAGT 
GCACCTTCAT ATCCTGGGTA CCTTCGAATC GTGAGGTTCT TGGATAATTC TCTTGATACC 
TTTCTAAATC GTCCTCCTGG ATTTCTCCAG GTTTGTGACT GGTTGTATCC TCTAGTTCCT 
GATAGGTCTC CAGTTCTTAA ATGTACTGTA GGTGCCTACA TGTTTCCTGA TACGATGTTA 
CAAGCATCGG GATGCTTTGT GGGGGTCGTC TTGTCATCTG AATTACCAGA AGATGACAGA 
GAACTCTTCG AGGATCTATT AAGACAAATG TCTGACCTTC GGCTCCAGAC CAACTGGGAC 
CGGGCAGAAG GAGAAAATGA ATTCCAGATC CCTGGAATAT CAGGATCTGC CTCTGACCAG 
TTGAAGGAAG CCAGTGGCAC TGACGTGAGA CAGTTGGACC CAAGCAGTAA AGATGTGCGT 
CAGAAGGGAA AACGAGGGAA AAAGATTCTG TGTTGTGTAC CAAATGTTGA ATTTGGATTA 
GAGACCAAAG GAACTTCAAG TGAAGAAGTT AATCTGAGTC ACATTGTACC CTGTGAGCCA 
GTTTCAGAAG AAAAAGCAAA GGAGTTACCT GAATGGAGTG AGAAAGTGGC TCACAACATT 
TTGTCAGGTG CTTCCTGGGT GAGTTGGGGT TTGGTCAAAG GTGCTGAGTT TACTGGCAAA 
GCCATCCAGA AAGGAGCGTC TAAACTTCGA GAACGAATTC AACCAGAAGA AAAGCCTGTG 
GAAGTTAGTC CAGCTGTGAC CAAGGGACTT TATATGGCAA AGCAGGCGAC TGGAGGAGCA 
GCCAAAGTCA GTCAGTTTCT GGTTGATGGA GTTTGTACTG TGGCAAATTG TGTTGGAAAG 
GAATTAGCTC CACACGTCAA GAAGCACGGA AGCAAACTTG TTCCAGAATC TCTTAAAAAA 
GACCGAAATG GAAAGTCTAC CCTGGATGGC GCTATGGTTG TAGCAGCAAG TAGTGTTCAA 
GGATTCTCAA CTGTCTGGCA AGGATTGGAA TGTGCAGCTA AATGCATTGT TAACAATGTT 
TCAGCAGAAA CTGTACAAAC TGTCAGATAC AAATATGGGC ATACTGCAGG AGAAGCTACA 
CACAACGCAG TAGATTCTGC CATCAATGTT GGTGTAACTG CCTATAATAT TGACAACATT 
GGCATCAAAG CAATGGTGAA GAAAACTGCA AAACAAACAG GACACACACT GCTTGAGGAC 
TATAAAATCA TTGATAATTC TAAGGGGGAA AATCCAGGAG GAGGAGCAAG TGCAAACCTG 
AAAGGGGAGA AAGATGAGCA GAAAGAGGGG CCAGAGAAGA ACGGGGCAAA GAAGAAAGAT 
AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005213700.1
CDS	189..2234
Translation	MEQGPQDGEPIEIKIIKEAYKKAFVFVNKGLNTDELGQKEEAKNYYKQGIGHLLRGISISSTDPEYTGPEWESARQMQQKMKETLQNVRTRLEILEKGLATSLRNDLQEVPKLYPEFPPKDMSEKSPEPQSLSSLPQHSEVNGSTSTASAESSSTPTTLSLPCQSHPSEAPPAYTPQAAEGHYTVSYGTESGEFSSVGENFYRNHSQPPPLETLGLDADELILIPNGVQIFFVNPAGEVSAPSYPGYLRIVRFLDNSLDTFLNRPPGFLQVCDWLYPLVPDRSPVLKCTVGAYMFPDTMLQASGCFVGVVLSSELPEDDRELFEDLLRQMSDLRLQTNWDRAEGENEFQIPGISGSASDQLKEASGTDVRQLDPSSKDVRQKGKRGKKILCCVPNVEFGLETKGTSSEEVNLSHIVPCEPVSEEKAKELPEWSEKVAHNILSGASWVSWGLVKGAEFTGKAIQKGASKLRERIQPEEKPVEVSPAVTKGLYMAKQATGGAAKVSQFLVDGVCTVANCVGKELAPHVKKHGSKLVPESLKKDRNGKSTLDGAMVVAASSVQGFSTVWQGLECAAKCIVNNVSAETVQTVRYKYGHTAGEATHNAVDSAINVGVTAYNIDNIGIKAMVKKTAKQTGHTLLEDYKIIDNSKGENPGGGASANLKGEKDEQKEGPEKNGAKKKDK

Target ORF information:

RefSeq Version	XM_005213643.3
Organism	Bos taurus(cattle)
Definition	Bos taurus spastic paraplegia 20 (Troyer syndrome) (SPG20), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005213643.3

ORF Insert Sequence:

ATGGAACAAG GACCCCAAGA TGGAGAACCT ATTGAAATTA AGATCATCAA GGAAGCATAC 
AAGAAAGCCT TTGTATTTGT TAATAAAGGA CTGAATACAG ATGAATTAGG TCAAAAGGAA 
GAAGCAAAGA ACTACTATAA GCAAGGAATA GGACACCTGC TTAGAGGAAT CAGCATTTCA 
TCAACAGATC CTGAGTACAC AGGTCCTGAG TGGGAGTCTG CCAGGCAGAT GCAACAGAAA 
ATGAAAGAAA CGCTACAGAA TGTACGTACC AGGTTGGAAA TTCTAGAGAA GGGTCTTGCC 
ACTTCTCTAC GGAATGATCT TCAGGAGGTG CCCAAGTTGT ATCCAGAATT TCCACCTAAA 
GACATGTCTG AAAAGTCACC AGAGCCTCAG AGCCTTAGTT CACTTCCTCA GCACAGTGAA 
GTAAATGGCA GCACTTCAAC TGCAAGTGCA GAATCAAGTA GTACACCTAC TACTTTGTCC 
TTGCCGTGTC AGAGTCATCC GTCCGAAGCA CCTCCTGCTT ACACTCCCCA GGCTGCTGAG 
GGTCACTACA CTGTATCCTA TGGAACAGAA TCTGGGGAGT TCTCGTCAGT TGGGGAGAAC 
TTCTATAGGA ATCACTCTCA GCCACCTCCT CTTGAGACCT TAGGGCTGGA TGCAGATGAG 
TTGATTTTGA TACCAAACGG AGTACAAATT TTCTTTGTAA ATCCCGCAGG GGAGGTTAGT 
GCACCTTCAT ATCCTGGGTA CCTTCGAATC GTGAGGTTCT TGGATAATTC TCTTGATACC 
TTTCTAAATC GTCCTCCTGG ATTTCTCCAG GTTTGTGACT GGTTGTATCC TCTAGTTCCT 
GATAGGTCTC CAGTTCTTAA ATGTACTGTA GGTGCCTACA TGTTTCCTGA TACGATGTTA 
CAAGCATCGG GATGCTTTGT GGGGGTCGTC TTGTCATCTG AATTACCAGA AGATGACAGA 
GAACTCTTCG AGGATCTATT AAGACAAATG TCTGACCTTC GGCTCCAGAC CAACTGGGAC 
CGGGCAGAAG GAGAAAATGA ATTCCAGATC CCTGGAATAT CAGGATCTGC CTCTGACCAG 
TTGAAGGAAG CCAGTGGCAC TGACGTGAGA CAGTTGGACC CAAGCAGTAA AGATGTGCGT 
CAGAAGGGAA AACGAGGGAA AAAGATTCTG TGTTGTGTAC CAAATGTTGA ATTTGGATTA 
GAGACCAAAG GAACTTCAAG TGAAGAAGTT AATCTGAGTC ACATTGTACC CTGTGAGCCA 
GTTTCAGAAG AAAAAGCAAA GGAGTTACCT GAATGGAGTG AGAAAGTGGC TCACAACATT 
TTGTCAGGTG CTTCCTGGGT GAGTTGGGGT TTGGTCAAAG GTGCTGAGTT TACTGGCAAA 
GCCATCCAGA AAGGAGCGTC TAAACTTCGA GAACGAATTC AACCAGAAGA AAAGCCTGTG 
GAAGTTAGTC CAGCTGTGAC CAAGGGACTT TATATGGCAA AGCAGGCGAC TGGAGGAGCA 
GCCAAAGTCA GTCAGTTTCT GGTTGATGGA GTTTGTACTG TGGCAAATTG TGTTGGAAAG 
GAATTAGCTC CACACGTCAA GAAGCACGGA AGCAAACTTG TTCCAGAATC TCTTAAAAAA 
GACCGAAATG GAAAGTCTAC CCTGGATGGC GCTATGGTTG TAGCAGCAAG TAGTGTTCAA 
GGATTCTCAA CTGTCTGGCA AGGATTGGAA TGTGCAGCTA AATGCATTGT TAACAATGTT 
TCAGCAGAAA CTGTACAAAC TGTCAGATAC AAATATGGGC ATACTGCAGG AGAAGCTACA 
CACAACGCAG TAGATTCTGC CATCAATGTT GGTGTAACTG CCTATAATAT TGACAACATT 
GGCATCAAAG CAATGGTGAA GAAAACTGCA AAACAAACAG GACACACACT GCTTGAGGAC 
TATAAAATCA TTGATAATTC TAAGGGGGAA AATCCAGGAG GAGGAGCAAG TGCAAACCTG 
AAAGGGGAGA AAGATGAGCA GAAAGAGGGG CCAGAGAAGA ACGGGGCAAA GAAGAAAGAT 
AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPART ( XM_005213643.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005213700.1 Bos taurus spartin (SPART), transcript variant X4, mRNA.

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CloneID	OBa24682
Clone ID Related Accession (Same CDS sequence)	XM_005213643.3 , XM_005213643.4
Accession Version	XM_005213643.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2046bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	spartin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037339.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005213643.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAACAAG GACCCCAAGA TGGAGAACCT ATTGAAATTA AGATCATCAA GGAAGCATAC 
AAGAAAGCCT TTGTATTTGT TAATAAAGGA CTGAATACAG ATGAATTAGG TCAAAAGGAA 
GAAGCAAAGA ACTACTATAA GCAAGGAATA GGACACCTGC TTAGAGGAAT CAGCATTTCA 
TCAACAGATC CTGAGTACAC AGGTCCTGAG TGGGAGTCTG CCAGGCAGAT GCAACAGAAA 
ATGAAAGAAA CGCTACAGAA TGTACGTACC AGGTTGGAAA TTCTAGAGAA GGGTCTTGCC 
ACTTCTCTAC GGAATGATCT TCAGGAGGTG CCCAAGTTGT ATCCAGAATT TCCACCTAAA 
GACATGTCTG AAAAGTCACC AGAGCCTCAG AGCCTTAGTT CACTTCCTCA GCACAGTGAA 
GTAAATGGCA GCACTTCAAC TGCAAGTGCA GAATCAAGTA GTACACCTAC TACTTTGTCC 
TTGCCGTGTC AGAGTCATCC GTCCGAAGCA CCTCCTGCTT ACACTCCCCA GGCTGCTGAG 
GGTCACTACA CTGTATCCTA TGGAACAGAA TCTGGGGAGT TCTCGTCAGT TGGGGAGAAC 
TTCTATAGGA ATCACTCTCA GCCACCTCCT CTTGAGACCT TAGGGCTGGA TGCAGATGAG 
TTGATTTTGA TACCAAACGG AGTACAAATT TTCTTTGTAA ATCCCGCAGG GGAGGTTAGT 
GCACCTTCAT ATCCTGGGTA CCTTCGAATC GTGAGGTTCT TGGATAATTC TCTTGATACC 
TTTCTAAATC GTCCTCCTGG ATTTCTCCAG GTTTGTGACT GGTTGTATCC TCTAGTTCCT 
GATAGGTCTC CAGTTCTTAA ATGTACTGTA GGTGCCTACA TGTTTCCTGA TACGATGTTA 
CAAGCATCGG GATGCTTTGT GGGGGTCGTC TTGTCATCTG AATTACCAGA AGATGACAGA 
GAACTCTTCG AGGATCTATT AAGACAAATG TCTGACCTTC GGCTCCAGAC CAACTGGGAC 
CGGGCAGAAG GAGAAAATGA ATTCCAGATC CCTGGAATAT CAGGATCTGC CTCTGACCAG 
TTGAAGGAAG CCAGTGGCAC TGACGTGAGA CAGTTGGACC CAAGCAGTAA AGATGTGCGT 
CAGAAGGGAA AACGAGGGAA AAAGATTCTG TGTTGTGTAC CAAATGTTGA ATTTGGATTA 
GAGACCAAAG GAACTTCAAG TGAAGAAGTT AATCTGAGTC ACATTGTACC CTGTGAGCCA 
GTTTCAGAAG AAAAAGCAAA GGAGTTACCT GAATGGAGTG AGAAAGTGGC TCACAACATT 
TTGTCAGGTG CTTCCTGGGT GAGTTGGGGT TTGGTCAAAG GTGCTGAGTT TACTGGCAAA 
GCCATCCAGA AAGGAGCGTC TAAACTTCGA GAACGAATTC AACCAGAAGA AAAGCCTGTG 
GAAGTTAGTC CAGCTGTGAC CAAGGGACTT TATATGGCAA AGCAGGCGAC TGGAGGAGCA 
GCCAAAGTCA GTCAGTTTCT GGTTGATGGA GTTTGTACTG TGGCAAATTG TGTTGGAAAG 
GAATTAGCTC CACACGTCAA GAAGCACGGA AGCAAACTTG TTCCAGAATC TCTTAAAAAA 
GACCGAAATG GAAAGTCTAC CCTGGATGGC GCTATGGTTG TAGCAGCAAG TAGTGTTCAA 
GGATTCTCAA CTGTCTGGCA AGGATTGGAA TGTGCAGCTA AATGCATTGT TAACAATGTT 
TCAGCAGAAA CTGTACAAAC TGTCAGATAC AAATATGGGC ATACTGCAGG AGAAGCTACA 
CACAACGCAG TAGATTCTGC CATCAATGTT GGTGTAACTG CCTATAATAT TGACAACATT 
GGCATCAAAG CAATGGTGAA GAAAACTGCA AAACAAACAG GACACACACT GCTTGAGGAC 
TATAAAATCA TTGATAATTC TAAGGGGGAA AATCCAGGAG GAGGAGCAAG TGCAAACCTG 
AAAGGGGAGA AAGATGAGCA GAAAGAGGGG CCAGAGAAGA ACGGGGCAAA GAAGAAAGAT 
AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005213700.1
CDS	127..2172
Translation	MEQGPQDGEPIEIKIIKEAYKKAFVFVNKGLNTDELGQKEEAKNYYKQGIGHLLRGISISSTDPEYTGPEWESARQMQQKMKETLQNVRTRLEILEKGLATSLRNDLQEVPKLYPEFPPKDMSEKSPEPQSLSSLPQHSEVNGSTSTASAESSSTPTTLSLPCQSHPSEAPPAYTPQAAEGHYTVSYGTESGEFSSVGENFYRNHSQPPPLETLGLDADELILIPNGVQIFFVNPAGEVSAPSYPGYLRIVRFLDNSLDTFLNRPPGFLQVCDWLYPLVPDRSPVLKCTVGAYMFPDTMLQASGCFVGVVLSSELPEDDRELFEDLLRQMSDLRLQTNWDRAEGENEFQIPGISGSASDQLKEASGTDVRQLDPSSKDVRQKGKRGKKILCCVPNVEFGLETKGTSSEEVNLSHIVPCEPVSEEKAKELPEWSEKVAHNILSGASWVSWGLVKGAEFTGKAIQKGASKLRERIQPEEKPVEVSPAVTKGLYMAKQATGGAAKVSQFLVDGVCTVANCVGKELAPHVKKHGSKLVPESLKKDRNGKSTLDGAMVVAASSVQGFSTVWQGLECAAKCIVNNVSAETVQTVRYKYGHTAGEATHNAVDSAINVGVTAYNIDNIGIKAMVKKTAKQTGHTLLEDYKIIDNSKGENPGGGASANLKGEKDEQKEGPEKNGAKKKDK

Target ORF information:

RefSeq Version	XM_005213643.4
Organism	Bos taurus(cattle)
Definition	Bos taurus spartin (SPART), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005213643.4

ORF Insert Sequence:

ATGGAACAAG GACCCCAAGA TGGAGAACCT ATTGAAATTA AGATCATCAA GGAAGCATAC 
AAGAAAGCCT TTGTATTTGT TAATAAAGGA CTGAATACAG ATGAATTAGG TCAAAAGGAA 
GAAGCAAAGA ACTACTATAA GCAAGGAATA GGACACCTGC TTAGAGGAAT CAGCATTTCA 
TCAACAGATC CTGAGTACAC AGGTCCTGAG TGGGAGTCTG CCAGGCAGAT GCAACAGAAA 
ATGAAAGAAA CGCTACAGAA TGTACGTACC AGGTTGGAAA TTCTAGAGAA GGGTCTTGCC 
ACTTCTCTAC GGAATGATCT TCAGGAGGTG CCCAAGTTGT ATCCAGAATT TCCACCTAAA 
GACATGTCTG AAAAGTCACC AGAGCCTCAG AGCCTTAGTT CACTTCCTCA GCACAGTGAA 
GTAAATGGCA GCACTTCAAC TGCAAGTGCA GAATCAAGTA GTACACCTAC TACTTTGTCC 
TTGCCGTGTC AGAGTCATCC GTCCGAAGCA CCTCCTGCTT ACACTCCCCA GGCTGCTGAG 
GGTCACTACA CTGTATCCTA TGGAACAGAA TCTGGGGAGT TCTCGTCAGT TGGGGAGAAC 
TTCTATAGGA ATCACTCTCA GCCACCTCCT CTTGAGACCT TAGGGCTGGA TGCAGATGAG 
TTGATTTTGA TACCAAACGG AGTACAAATT TTCTTTGTAA ATCCCGCAGG GGAGGTTAGT 
GCACCTTCAT ATCCTGGGTA CCTTCGAATC GTGAGGTTCT TGGATAATTC TCTTGATACC 
TTTCTAAATC GTCCTCCTGG ATTTCTCCAG GTTTGTGACT GGTTGTATCC TCTAGTTCCT 
GATAGGTCTC CAGTTCTTAA ATGTACTGTA GGTGCCTACA TGTTTCCTGA TACGATGTTA 
CAAGCATCGG GATGCTTTGT GGGGGTCGTC TTGTCATCTG AATTACCAGA AGATGACAGA 
GAACTCTTCG AGGATCTATT AAGACAAATG TCTGACCTTC GGCTCCAGAC CAACTGGGAC 
CGGGCAGAAG GAGAAAATGA ATTCCAGATC CCTGGAATAT CAGGATCTGC CTCTGACCAG 
TTGAAGGAAG CCAGTGGCAC TGACGTGAGA CAGTTGGACC CAAGCAGTAA AGATGTGCGT 
CAGAAGGGAA AACGAGGGAA AAAGATTCTG TGTTGTGTAC CAAATGTTGA ATTTGGATTA 
GAGACCAAAG GAACTTCAAG TGAAGAAGTT AATCTGAGTC ACATTGTACC CTGTGAGCCA 
GTTTCAGAAG AAAAAGCAAA GGAGTTACCT GAATGGAGTG AGAAAGTGGC TCACAACATT 
TTGTCAGGTG CTTCCTGGGT GAGTTGGGGT TTGGTCAAAG GTGCTGAGTT TACTGGCAAA 
GCCATCCAGA AAGGAGCGTC TAAACTTCGA GAACGAATTC AACCAGAAGA AAAGCCTGTG 
GAAGTTAGTC CAGCTGTGAC CAAGGGACTT TATATGGCAA AGCAGGCGAC TGGAGGAGCA 
GCCAAAGTCA GTCAGTTTCT GGTTGATGGA GTTTGTACTG TGGCAAATTG TGTTGGAAAG 
GAATTAGCTC CACACGTCAA GAAGCACGGA AGCAAACTTG TTCCAGAATC TCTTAAAAAA 
GACCGAAATG GAAAGTCTAC CCTGGATGGC GCTATGGTTG TAGCAGCAAG TAGTGTTCAA 
GGATTCTCAA CTGTCTGGCA AGGATTGGAA TGTGCAGCTA AATGCATTGT TAACAATGTT 
TCAGCAGAAA CTGTACAAAC TGTCAGATAC AAATATGGGC ATACTGCAGG AGAAGCTACA 
CACAACGCAG TAGATTCTGC CATCAATGTT GGTGTAACTG CCTATAATAT TGACAACATT 
GGCATCAAAG CAATGGTGAA GAAAACTGCA AAACAAACAG GACACACACT GCTTGAGGAC 
TATAAAATCA TTGATAATTC TAAGGGGGAA AATCCAGGAG GAGGAGCAAG TGCAAACCTG 
AAAGGGGAGA AAGATGAGCA GAAAGAGGGG CCAGAGAAGA ACGGGGCAAA GAAGAAAGAT 
AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

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SPART cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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