PYK4a cDNA ORF clone, Chlamydomonas reinhardtii

Gene
Clones
gRNAs

Summary

Gene Symbol	PYK4a
Entrez Gene ID	5721287
Full Name	pyruvate kinase
Gene Type	protein-coding
Organism	Chlamydomonas reinhardtii

mRNA and Protein(s)

mRNA	Protein	Name
XM_001695686.1	XP_001695738.1	pyruvate kinase

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The Chlamydomonas genome reveals the evolution of key animal and plant functions.
Merchant SS, Prochnik SE, Vallon O, Harris EH, Karpowicz SJ, Witman GB, Terry A, Salamov A, Fritz-Laylin LK, Mar?chal-Drouard L, Marshall WF, Qu LH, Nelson DR, Sanderfoot AA, Spalding MH, Kapitonov VV, Ren Q, Ferris P, Lindquist E, Shapiro H, Lucas SM, Grimwood J, Schmutz J, Cardol P, Cerutti H, Chanfreau G, Chen CL, Cognat V, Croft MT, Dent R, Dutcher S, Fern?ndez E, Fukuzawa H, Gonz?lez-Ballester D, Gonz?lez-Halphen D, Hallmann A, Hanikenne M, Hippler M, Inwood W, Jabbari K, Kalanon M, Kuras R, Lefebvre PA, Lemaire SD, Lobanov AV, Lohr M, Manuell A, Meier I, Mets L, Mittag M, Mittelmeier T, Moroney JV, Moseley J, Napoli C, Nedelcu AM, Niyogi K, Novoselov SV, Paulsen IT, Pazour G, Purton S, Ral JP, Ria?o-Pach?n DM, Riekhof W, Rymarquis L, Schroda M, Stern D, Umen J, Willows R, Wilson N, Zimmer SL, Allmer J, Balk J, Bisova K, Chen CJ, Elias M, Gendler K, Hauser C, Lamb MR, Ledford H, Long JC, Minagawa J, Page MD, Pan J, Pootakham W, Roje S, Rose A, Stahlberg E, Terauchi AM, Yang P, Ball S, Bowler C, Dieckmann CL, Gladyshev VN, Green P, Jorgensen R, Mayfield S, Mueller-Roeber B, Rajamani S, Sayre RT, Brokstein P, Dubchak I, Goodstein D, Hornick L, Huang YW, Jhaveri J, Luo Y, Mart?nez D, Ngau WC, Otillar B, Poliakov A, Porter A, Szajkowski L, Werner G, Zhou K, Grigoriev IV, Rokhsar DS, Grossman AR
Science (New York, N.Y.)318(5848)245-50(2007 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following PYK4a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PYK4a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCh04060	XM_001695686.1 Latest version!	Chlamydomonas reinhardtii pyruvate kinase (PYK4a), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PYK4a ( XM_001695686.1 ) cDNA ORF clone, Chlamydomonas reinhardtii -> XP_001695738.1 Chlamydomonas reinhardtii pyruvate kinase (PYK4a), partial mRNA.

Price & Availability↑

CloneID	OCh04060
Clone ID Related Accession (Same CDS sequence)	XM_001695686.1
Accession Version	XM_001695686.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7293bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-08-28
Organism	Chlamydomonas reinhardtii
Product	pyruvate kinase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001843709). COMPLETENESS: incomplete on both ends.

ATGAAAATCA GCAGCACCGT GTCGTTTGGG CAGCTGACGG AGGAGGTCAG CAAGCCCCTG 
TCCAAGGCCC GCTCCGCGCT GAAGAGCGTG CACAGCCGCA TGGACCTCAT CCAGGAGTAC 
CACCTGCGTG AGGTGGCGGC TGCGGCGCTG AAGCGCGAGA AGGCCATGGA CTGGCTGCCC 
GAGTACCGGC CCATCAGCGT GGTGCCGCCC TCCCGCGGCC GCCTCACGCA CCTGGAGCGC 
ACCACGCGCG TCAGCCAGGC CGCCATCCTC AACGACGTGC CTGTGGGCCG CAAGACCAAG 
ATCGTGTGCA CGCTGGGCCC CAGCTGCTGG TCCGAGGAGG GCCTGGGCGG GCTGCTGGAC 
GCGGGCATGG ACGTGGCGCG CTTCAACTTC AGCCACGGCA CTCACCAGGC GCACCAGGAG 
GTGCTGGACC GCCTGCGCAA GGTAGTGGAG GCGAAGAAGC GCCACACGGC CTTCCTGCTG 
GACACCAAGG GCCCCGAGAT CCGCACAGCC ATGCTCAAGG ACGGCAAGGA CATTGAGCTT 
GTCGCGGGGC AGGAGGTGAC CGTGGTGGGT GTCGGTGAGG ACTACGACAA GTGGGAGGGC 
TACAAGGACG AGAAGACGGG CGAGACCAAG ATCGGCATCA GCTACGCCAA GCTGTGCGCC 
AGCATCAAGG TCGGCGGCGT CATCCTCATT GCCGATGGCG CCATCAGCAT CGAGGTGGTC 
AAGATCCTCA ACGACCGCGA GCTGTTGGGC AGCGTCATCA ACAGCCACAA GCTGGGCCAG 
CGGAAGAACT GCAACCTACC CGGCGTGCAC GTGGACCTGC CGGTGCTCAG CCCCAAGGAC 
ATTGACGACG TCCAGAACTT CGCGGTGAAG AACCAGATGG ACTTTATTGC CGCGTCGTTC 
GTGCAGTGCG CGGATGATGT CAAGATGATT CGCCGGGTGC TGGACGACGC GGGCGGCTAC 
CACATCAAGA TTATCTGCAA GATTGAGAAC GAGGCGGGCC TGCGCAACAT CGACACCATC 
CTGGAGGTCA CGGACGGCAT CATGGTGGCG CGTGGCGACC TGGCCATGGA GGTGCCCGCG 
GAGAAGATCG CGCTGGCTCA GAAGATGATC ATTGCCAAGT GCAACGTGCT GGGCAAGACG 
GTCATCACCG CCACCCAGAT GCTGGAGTCC ATGACCGGCT CGCCGCTGCC CACTCGTGCG 
GAGATGACGG ACGTGGCCAA CGCCGTGTAC GACGGCACCG ACGCCGTCAT GCTCAGCGGC 
GAGACCGCCA ACGGCGCCTA CCCGCACGCC GCGGTTCGCA CCATGGCGCA CATCGTGGAG 
TACGCGGAGC TGGGTGTGGA CTACGCCTTC CACCACGACT GGGTCAAGCG CCACAACGCC 
GGCCTGGCTC CGGTCAGCCC CCTGGAGGCC ACACTGGCAT GTGTGGCCAA GTCTGCCATC 
ACGTTCTCCA TGGACGCTAA TGGCGATGGT GTGCTGGACG CATCGGAGGG CTGCGTGGCG 
GTCGTGTTCA CGCGCAGCGG CCTGGCGGCG CAGGTGATCG CCAAGTACCG GCCGCCCTGC 
CCGGTCATTG TGCTGAGCGA CCACGACTGG GTGCTGCGCC AGGCCAGCCT CACCTACGGC 
CTGTACCCGC TGCGGGTGGA GGTGTCGGGC ATGGAGGACC GCCGCAAGGC CGTGGAGACG 
GCCATCGAGT ACGGCAGGCA GCGCAAGCTG GTGTACGACG GCAAGAACAT CCTGGTGGTG 
ACGGGCAGCG GCGAGCCGTT CGCGGACACG GTGGCGGAGA TGTACGTGGA CCAGATCGGC 
TCTGAGGGCG GAGCCGGCTG GGTGCGCCGC GCCGTCAGTT GCAAGCGCAG CCTGAGCGTC 
GGCCAGCCCA TGCACATCGA GTACAAGTCG CCCTTCGCCG GTAACTCGGT GGTCTGCGTG 
CAGTCTCTGC GCATCACGCC GGAGCTCATC ATTGAGCGCC AGTTCAACCA CCGCTCCACC 
AAGATCTTCG CCAGCCTGGG CCCCGCCAGC TACGACCCCA AGAAGGTGGA GGCCATGCTG 
GACAACGGCG TCAGCGTGTT CCGCATCTTC CTGCACGAGG CACCCACCGA CTTCTACCAG 
AAGCTGGTGG ACCGGCTCCG CGAGCAGTTC GAGCAGCGCC GCGAGAAGTG CTCCTACACC 
ACCATGCCCG GCCTCATGGC CACCATCAAG ACGGTGGTGC TGGTGCCCAC CTCGGACCCC 
CTATTCGAGG GCTTCACCGG CGCCGACGGC ATTCACATTG TGGGCGTCAA CTTCCCCACC 
CTCACCGCCA AGCTGCGCGC AGGCGACGTG CTGGTGCTGG CGGAGGGCAC CATCTCCCTG 
ACTGTGAAGG AGGTGGTCCC GCGCCTGGCG GCCGTTGCGC CCGGCGGCGG CGCTGACGGC 
AGTGTTGCGT ACATGCACGG CAAGGTGATC ACCAAGGTCA CCACAGCGGC CAAGCTGGGT 
GAGGACCGCG CCATCGACCT CGCGGGTGGC TGCCTGCTGC AGGACAACTT CCTGGGCGAC 
CGCGACTGCG AGGCCGTCCT GTTCTGCGCC AAGAATATGA TCGACTTCGT GAGCATCCCC 
AACGTGCGCT CGCGGGCGGA CGTGATGGCG GTGCGCGAGC TCCTGGACGC CAACGGCGGC 
GCGCGCTGCA AGATCATCTC GCACGTGTCC ACGGCCAAGG CCATCGCCAA CTACGATGAG 
ATCCTGGATG CCACCGACGC CGTGCTCATC TCGCGCGCGT ACATGGGCAT GCGCATCCCC 
GCGGCCAAGG TGTCCCTGGC CCAGAAGTGG ATGACTGCCA AGGCCAACCT GAAGGGCAAG 
CCGTGCATCG TCAGCGCGCA GATCATGTAT AGCATGGTGG ACAGCCCCCG CCCCACCCGC 
GCCGAGATGA CGGACGTGGC CAACGCGCTG TACGACGGCG CCGACGCGCT GATGCTGCGC 
GAGGAGACCG CCGTGGGCTC GTTCGTGGAG CGCGTGGTGT CCATCACCGC CGACATCCTG 
CGCGACGCCG AGGTCGGAGT GGACACCTAC GCGCAGCTCA ACCAGCTGCG CAACTTCACG 
CCTAAGCCCA TGCCCACCCT GGAGTGCCTG TGTTCCTCGG CGGTCAAGGC GGCGGTGGAC 
ATGCGCGCGG CGCTGATTGC CGTCATCACC GACACCAGCG CGCCCGTGCG CGCCCTGGCC 
AAGTACCGCC CTGGCCAGGC CATTGTGGTG GTGACCACGC GCACGCACGT GGCGCGCCAG 
TGCAACATGA ACTACGGCTG CGTGCCGCTG CTGCTCCGCA GCAAGGACCG CGACGCGCCG 
CTGGACAAGA TCGTGAGCAA GATCGTGGAC TTTGCGCGCA CCCAGAAGCT GGCTGCATTC 
CAGGCCGGCG ACGACCAGGG CGACCAGCTC ATCATCGTGC AGGGCCCCTC CAACCGCACC 
TTTGCCACGG AGGAGGACCT GGCGGAGATG CAGTTCGTGA CGCGCATTAT GGGCGAGGAG 
GCGTCCGAGG TGTGCGCCAA GCTCGGCTAC ACTGGCGCTG ACACCATTGC CTACCGCTCC 
ACCAAGGTGG GCCTGGACCT CATCTGCGGC CCCACCGACA CGCGCACCCG CAAGACTAAG 
GTGGTGTGCA CGCTGGGCCC CAAGTGCTGG AGCAAGGAGG GCCTGGAGAT GCTGGTGGAC 
ACCGGCATCA ACGTGGCCAG GTTCAATTTC AGCCACGGCA GCCACGCCGA CCACCAGGAG 
GTGCTGGACC GCCTGCGCCT GGTGCTGGAC GAGCGCGGCG CGCGGCACCG TGTGGCGCTG 
CTGCTGGACA CCAAGGGCCC CGAGGTGCGC ACCGCCATGC TCAAGGACGG CAAGGACATC 
AACCTGGAGG CGGGCCAGGA GCTGACGGTG GTGGCGGTAG GCGACAAGTA CACCACCTGG 
GAGGGCTTCA AGGACGAGGC GACGGGCGAG ACCAGGATTG GCCTCAGCTA CCAGCACCTG 
TGCCGCGATG TCAAGGTCGG CGGCATGATC AAGATCGGTG ACGGCCTCAT CACCCTGGAG 
GACGGTGGCG GTGGCATTGT GCACTTGCAG GTGCTGGAGA TTCTGTCGCC CACGGAGCTG 
AGGGTGCGCG CGCTGAACAG CAAGGCGCTG GGCCAGCGCA AGAACGTCAA CCTGCCGGGT 
GTGCACGTGG ACATGCCTGT GCTGGGCCCC AACGACATCA ACGACGTGCA GAACTTCGCC 
GCCAAGAACA ACATGGACTT TGTGGCGGCG TCCTTCGTGC AGAGCGCCGA TGACGTGCGC 
TTCATCCGCA AGGTGCTGGA CGAGGCGGGC GGCAAGAACG TGCGCATCAT CTCCAAGATC 
GAGTCCACCG CCGGCCTGAT CAACTACGAC GCCATCCTGG CTGAGAGCGA CGGCATTATG 
GTGGCACGTG GCGACCTGGC CATGGAGATT CCCTCGGAGA AGGTGGCTCT GGCCCAGAAG 
ATGATGATCA CCAAGGCCAA CATTGCGGGC AAGTTCGTCA TCACCGCCAC CCAGATGCTG 
GAGTCCATGA TTAAATCGCC GCTGCCCACT CGTGCGGAGA TGACGGACGT GGCCAACGCC 
GTGTTCGATG GCACCGACGC CGTCATGCTC AGCGGCGAGA CCGCCAACGG CGACTTCCCC 
CAGCTGGCGC TGGCGACCAT GTGCGCCATC TCCGCCAACG CAGAGGTGGG CACCAACTAC 
CCGCAGGTGT ACGACTTCAT CCGCGACTTC TCTGCGCGCC CCATGTCCAC CGCCGAGGCG 
GTGCTGGGCT GCGCCGCCAA GAACGTGCTG GACGTGGACG CGGCGCTCAT CCTGGTCATC 
AGCTCCACCG GTGCCAGTGC CCGCCTGGTG GCCAAGTACC GCCCGCGCGT GCCGGTGCTG 
CTCATCACCG ACTCCATGGC CGCCGCGCGC TCCGTGGCGC CGCTCTTTGG TGTGTACGTC 
AGCATCGTGG ACGAGCTGCC GCCCTCGCGC TTCGACCTGG AGTTTGAGGT GCTGCTGGAG 
GAGGCGACCC TGTTCGCCAT GGAGGCCGGC CTGTGCCCGC CCGGCAAGGA GGTGGTGGTG 
GTGCACGGCT GCAACACGGC GGACGCGGAG GACGGGCTGC CCATGGTGGA GATCCAGGTG 
GCGGCGGGCG GCGTGCCGTC GCGCCAGACC AGTGAGGCCA TCAGCGAGAC CGTCACGCCC 
AAGCCCAGCC TGCCGCAGCC CAAGGCCAAG CGCACGGGGC CCAGCCCCTT CGCGCCGGCC 
GTGCCGCCCA GCCCCGCCAC CACCGCCTTC CAGCACGCCT TTTACCTGGG CGGCGCGGGC 
ATTCACCACG TCACGTCTGG CGTGACGCCC TTCGGCAGCC TGGCAGCCCT GGCCCCGTCC 
GAGGCGCCGC CCTCGCCCAA GCAGCAGCCC CACGCCACCA ACAGGCTCGT CGCCGACGTG 
GTGGAGGAGG GCGCCTCCAG CGGCGCTTCA GCCGCCGCCG CCCTGCGCGA CAACCTGCCC 
GCCATCCCCG CCGCCGCCAA GCCCGCCAGC AACGTGGCCT CGCTCACGCC GTCGCCGGCG 
CCCACGGCCT TGCCCAAGGC CCTCACCGCG GCCGTGATGG CGGCCAAGAC GCTGAGCCTG 
CGCACCACCG CCATCAGCCT GGTGGACATC CTGGACCACA GCCTGCCGGC CGGCCGCAAG 
ACCAAGATCG TGTGCACGCT GGGCCCCAGC TGCTGGTCCG AGGAGGGCCT CAACGGCATC 
ATCAACGCGG GCATGGACGT GGCGCGCTTC AACTTCAGCC ATGGCACCCA CCAGGCGCAC 
CAGGAGGTGC TGGACCGCCT GCGCAAGGTG GAGAAGGCCG GCACCAAGCG AATCGCCTAC 
CTGCTGGACA CCAAGGGCCC CGAGGTGCGC ACCGCCATGC TGCGCGAGGG CAAGGACATC 
AAGCTGGACG CCAACCAGGA GGTCACGGTG GTGGCGGTGG GAGACGACTA CGACAAGTGG 
GAGGGCTACA AGGACGAGAA GACGGGCGAG ACCAAGATCG GCATCAGCTA CGCCAAGCTG 
TGCCAGAGCG TGCGCGTGGG CAGCCGCATC CTGGTGGCGG ACGGCTCGCT GACCATTGAG 
GTGCTGGAGA TCACGTCGGC CACGGAGCTG CGCGGCCGCT GCCTCAACAC CAAGGTGCTG 
GGCCAGCGCA AGAACGTCAA CCTGCCCGGC GTGCACGTGG ACATCCCCGT GCTGACGCCC 
AAGGACATCG AGGACCTGCA GAAGTTCTGT GTGGGCAACG ACATGGACTT CGTGGCGGCG 
TCCTTCGTGC AGAGCGCCGA CGACGTGCGC CTCATCCGCC GCGTCCTTGA CGAGGCGGGC 
GGCTACAACG TGCAGATCAT CTCCAAGATT GAGAACGAGG CGGGCCTCAA GTATTTTGAC 
GGCATCCTGG CTGAGAGCGA CGGCATCATG GTGGCGCGCG GCGACCTGGC CATGGAGATT 
CCCTCGGAGA AGGTGGCTCT GGCCCAGAAG ATGATGATCA CCAAGGCCAA CATTGCCGGC 
AAGTTCGTCA TCACCGCCAC CCAGATGCTG GAGTCCATGA CCAGCGCGCC GCTGCCCACT 
CGTGCGGAGA TGACGGACGT GGCCAACGCC GTGTTCGATG GCACCGACGC CGTCATGCTC 
AGCGGCGAGA CCGCCAACGG CAAGTTCCCG CGCCCGGCGG TGGAGACCAT GGCCGCCATC 
GTGGCCAACG CCGAGAACGG CACCGCCTAC GTGTCCACGC AGTGGTTCAT CCGCGACCAC 
ACGCCCAAGC CGTTCGGCAT CACGGAGGCC ACCGGCGTGT CGTGCGTGGC TGCGTCCGTG 
GACTGCAACG CGCAGCTGAT CATCACGCTC ACCAGCGGCG GCTACACCAG CCGCTTCGTG 
TCCAAGTACC GCCCCGCCGT GCCGCAGATT GTGGTGACGG ACTCGGTGCA CGTGGCCAAC 
CAGGCCAGTG TGCACTTCGG CCAGTACGCG CTGCTGGTGG GCGACATCGA CTTCAGCACG 
TGGCCGGCGG ACCGCAGCCT GACCGCCATG GTGGACGAGC TGGTGGCGCA GGCGGCGCTG 
TTCGCGCAGG ACAAGGGGCT GTGGGCCGGC CAGGGCACAG CCATCCTGCT GCACGGCCGC 
GACGACCTGT CGGCGGACAG CCAGCCCGTG CTGCGCGCCG TGGACCTGGC CGACCTCGTG 
GGCGCCGCGC CCGCCGAGCT CACCGCCGCC AGCAGCAAGC TGTCGGCGGG CTCGGGCCCC 
GGCCGCGCCG GCTCGCGTGA GGTAGGGCTG GGCCGCACGT CCGCCGGCCT GGCGGGCACG 
TCCGTGAACC GCGCCTTCAG CCTGGTGGTG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001695738.1
CDS	1..7293
Translation	MKISSTVSFGQLTEEVSKPLSKARSALKSVHSRMDLIQEYHLREVAAAALKREKAMDWLPEYRPISVVPPSRGRLTHLERTTRVSQAAILNDVPVGRKTKIVCTLGPSCWSEEGLGGLLDAGMDVARFNFSHGTHQAHQEVLDRLRKVVEAKKRHTAFLLDTKGPEIRTAMLKDGKDIELVAGQEVTVVGVGEDYDKWEGYKDEKTGETKIGISYAKLCASIKVGGVILIADGAISIEVVKILNDRELLGSVINSHKLGQRKNCNLPGVHVDLPVLSPKDIDDVQNFAVKNQMDFIAASFVQCADDVKMIRRVLDDAGGYHIKIICKIENEAGLRNIDTILEVTDGIMVARGDLAMEVPAEKIALAQKMIIAKCNVLGKTVITATQMLESMTGSPLPTRAEMTDVANAVYDGTDAVMLSGETANGAYPHAAVRTMAHIVEYAELGVDYAFHHDWVKRHNAGLAPVSPLEATLACVAKSAITFSMDANGDGVLDASEGCVAVVFTRSGLAAQVIAKYRPPCPVIVLSDHDWVLRQASLTYGLYPLRVEVSGMEDRRKAVETAIEYGRQRKLVYDGKNILVVTGSGEPFADTVAEMYVDQIGSEGGAGWVRRAVSCKRSLSVGQPMHIEYKSPFAGNSVVCVQSLRITPELIIERQFNHRSTKIFASLGPASYDPKKVEAMLDNGVSVFRIFLHEAPTDFYQKLVDRLREQFEQRREKCSYTTMPGLMATIKTVVLVPTSDPLFEGFTGADGIHIVGVNFPTLTAKLRAGDVLVLAEGTISLTVKEVVPRLAAVAPGGGADGSVAYMHGKVITKVTTAAKLGEDRAIDLAGGCLLQDNFLGDRDCEAVLFCAKNMIDFVSIPNVRSRADVMAVRELLDANGGARCKIISHVSTAKAIANYDEILDATDAVLISRAYMGMRIPAAKVSLAQKWMTAKANLKGKPCIVSAQIMYSMVDSPRPTRAEMTDVANALYDGADALMLREETAVGSFVERVVSITADILRDAEVGVDTYAQLNQLRNFTPKPMPTLECLCSSAVKAAVDMRAALIAVITDTSAPVRALAKYRPGQAIVVVTTRTHVARQCNMNYGCVPLLLRSKDRDAPLDKIVSKIVDFARTQKLAAFQAGDDQGDQLIIVQGPSNRTFATEEDLAEMQFVTRIMGEEASEVCAKLGYTGADTIAYRSTKVGLDLICGPTDTRTRKTKVVCTLGPKCWSKEGLEMLVDTGINVARFNFSHGSHADHQEVLDRLRLVLDERGARHRVALLLDTKGPEVRTAMLKDGKDINLEAGQELTVVAVGDKYTTWEGFKDEATGETRIGLSYQHLCRDVKVGGMIKIGDGLITLEDGGGGIVHLQVLEILSPTELRVRALNSKALGQRKNVNLPGVHVDMPVLGPNDINDVQNFAAKNNMDFVAASFVQSADDVRFIRKVLDEAGGKNVRIISKIESTAGLINYDAILAESDGIMVARGDLAMEIPSEKVALAQKMMITKANIAGKFVITATQMLESMIKSPLPTRAEMTDVANAVFDGTDAVMLSGETANGDFPQLALATMCAISANAEVGTNYPQVYDFIRDFSARPMSTAEAVLGCAAKNVLDVDAALILVISSTGASARLVAKYRPRVPVLLITDSMAAARSVAPLFGVYVSIVDELPPSRFDLEFEVLLEEATLFAMEAGLCPPGKEVVVVHGCNTADAEDGLPMVEIQVAAGGVPSRQTSEAISETVTPKPSLPQPKAKRTGPSPFAPAVPPSPATTAFQHAFYLGGAGIHHVTSGVTPFGSLAALAPSEAPPSPKQQPHATNRLVADVVEEGASSGASAAAALRDNLPAIPAAAKPASNVASLTPSPAPTALPKALTAAVMAAKTLSLRTTAISLVDILDHSLPAGRKTKIVCTLGPSCWSEEGLNGIINAGMDVARFNFSHGTHQAHQEVLDRLRKVEKAGTKRIAYLLDTKGPEVRTAMLREGKDIKLDANQEVTVVAVGDDYDKWEGYKDEKTGETKIGISYAKLCQSVRVGSRILVADGSLTIEVLEITSATELRGRCLNTKVLGQRKNVNLPGVHVDIPVLTPKDIEDLQKFCVGNDMDFVAASFVQSADDVRLIRRVLDEAGGYNVQIISKIENEAGLKYFDGILAESDGIMVARGDLAMEIPSEKVALAQKMMITKANIAGKFVITATQMLESMTSAPLPTRAEMTDVANAVFDGTDAVMLSGETANGKFPRPAVETMAAIVANAENGTAYVSTQWFIRDHTPKPFGITEATGVSCVAASVDCNAQLIITLTSGGYTSRFVSKYRPAVPQIVVTDSVHVANQASVHFGQYALLVGDIDFSTWPADRSLTAMVDELVAQAALFAQDKGLWAGQGTAILLHGRDDLSADSQPVLRAVDLADLVGAAPAELTAASSKLSAGSGPGRAGSREVGLGRTSAGLAGTSVNRAFSLVV

Target ORF information:

RefSeq Version	XM_001695686.1
Organism	Chlamydomonas reinhardtii
Definition	Chlamydomonas reinhardtii pyruvate kinase (PYK4a), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001695686.1

ORF Insert Sequence:

ATGAAAATCA GCAGCACCGT GTCGTTTGGG CAGCTGACGG AGGAGGTCAG CAAGCCCCTG 
TCCAAGGCCC GCTCCGCGCT GAAGAGCGTG CACAGCCGCA TGGACCTCAT CCAGGAGTAC 
CACCTGCGTG AGGTGGCGGC TGCGGCGCTG AAGCGCGAGA AGGCCATGGA CTGGCTGCCC 
GAGTACCGGC CCATCAGCGT GGTGCCGCCC TCCCGCGGCC GCCTCACGCA CCTGGAGCGC 
ACCACGCGCG TCAGCCAGGC CGCCATCCTC AACGACGTGC CTGTGGGCCG CAAGACCAAG 
ATCGTGTGCA CGCTGGGCCC CAGCTGCTGG TCCGAGGAGG GCCTGGGCGG GCTGCTGGAC 
GCGGGCATGG ACGTGGCGCG CTTCAACTTC AGCCACGGCA CTCACCAGGC GCACCAGGAG 
GTGCTGGACC GCCTGCGCAA GGTAGTGGAG GCGAAGAAGC GCCACACGGC CTTCCTGCTG 
GACACCAAGG GCCCCGAGAT CCGCACAGCC ATGCTCAAGG ACGGCAAGGA CATTGAGCTT 
GTCGCGGGGC AGGAGGTGAC CGTGGTGGGT GTCGGTGAGG ACTACGACAA GTGGGAGGGC 
TACAAGGACG AGAAGACGGG CGAGACCAAG ATCGGCATCA GCTACGCCAA GCTGTGCGCC 
AGCATCAAGG TCGGCGGCGT CATCCTCATT GCCGATGGCG CCATCAGCAT CGAGGTGGTC 
AAGATCCTCA ACGACCGCGA GCTGTTGGGC AGCGTCATCA ACAGCCACAA GCTGGGCCAG 
CGGAAGAACT GCAACCTACC CGGCGTGCAC GTGGACCTGC CGGTGCTCAG CCCCAAGGAC 
ATTGACGACG TCCAGAACTT CGCGGTGAAG AACCAGATGG ACTTTATTGC CGCGTCGTTC 
GTGCAGTGCG CGGATGATGT CAAGATGATT CGCCGGGTGC TGGACGACGC GGGCGGCTAC 
CACATCAAGA TTATCTGCAA GATTGAGAAC GAGGCGGGCC TGCGCAACAT CGACACCATC 
CTGGAGGTCA CGGACGGCAT CATGGTGGCG CGTGGCGACC TGGCCATGGA GGTGCCCGCG 
GAGAAGATCG CGCTGGCTCA GAAGATGATC ATTGCCAAGT GCAACGTGCT GGGCAAGACG 
GTCATCACCG CCACCCAGAT GCTGGAGTCC ATGACCGGCT CGCCGCTGCC CACTCGTGCG 
GAGATGACGG ACGTGGCCAA CGCCGTGTAC GACGGCACCG ACGCCGTCAT GCTCAGCGGC 
GAGACCGCCA ACGGCGCCTA CCCGCACGCC GCGGTTCGCA CCATGGCGCA CATCGTGGAG 
TACGCGGAGC TGGGTGTGGA CTACGCCTTC CACCACGACT GGGTCAAGCG CCACAACGCC 
GGCCTGGCTC CGGTCAGCCC CCTGGAGGCC ACACTGGCAT GTGTGGCCAA GTCTGCCATC 
ACGTTCTCCA TGGACGCTAA TGGCGATGGT GTGCTGGACG CATCGGAGGG CTGCGTGGCG 
GTCGTGTTCA CGCGCAGCGG CCTGGCGGCG CAGGTGATCG CCAAGTACCG GCCGCCCTGC 
CCGGTCATTG TGCTGAGCGA CCACGACTGG GTGCTGCGCC AGGCCAGCCT CACCTACGGC 
CTGTACCCGC TGCGGGTGGA GGTGTCGGGC ATGGAGGACC GCCGCAAGGC CGTGGAGACG 
GCCATCGAGT ACGGCAGGCA GCGCAAGCTG GTGTACGACG GCAAGAACAT CCTGGTGGTG 
ACGGGCAGCG GCGAGCCGTT CGCGGACACG GTGGCGGAGA TGTACGTGGA CCAGATCGGC 
TCTGAGGGCG GAGCCGGCTG GGTGCGCCGC GCCGTCAGTT GCAAGCGCAG CCTGAGCGTC 
GGCCAGCCCA TGCACATCGA GTACAAGTCG CCCTTCGCCG GTAACTCGGT GGTCTGCGTG 
CAGTCTCTGC GCATCACGCC GGAGCTCATC ATTGAGCGCC AGTTCAACCA CCGCTCCACC 
AAGATCTTCG CCAGCCTGGG CCCCGCCAGC TACGACCCCA AGAAGGTGGA GGCCATGCTG 
GACAACGGCG TCAGCGTGTT CCGCATCTTC CTGCACGAGG CACCCACCGA CTTCTACCAG 
AAGCTGGTGG ACCGGCTCCG CGAGCAGTTC GAGCAGCGCC GCGAGAAGTG CTCCTACACC 
ACCATGCCCG GCCTCATGGC CACCATCAAG ACGGTGGTGC TGGTGCCCAC CTCGGACCCC 
CTATTCGAGG GCTTCACCGG CGCCGACGGC ATTCACATTG TGGGCGTCAA CTTCCCCACC 
CTCACCGCCA AGCTGCGCGC AGGCGACGTG CTGGTGCTGG CGGAGGGCAC CATCTCCCTG 
ACTGTGAAGG AGGTGGTCCC GCGCCTGGCG GCCGTTGCGC CCGGCGGCGG CGCTGACGGC 
AGTGTTGCGT ACATGCACGG CAAGGTGATC ACCAAGGTCA CCACAGCGGC CAAGCTGGGT 
GAGGACCGCG CCATCGACCT CGCGGGTGGC TGCCTGCTGC AGGACAACTT CCTGGGCGAC 
CGCGACTGCG AGGCCGTCCT GTTCTGCGCC AAGAATATGA TCGACTTCGT GAGCATCCCC 
AACGTGCGCT CGCGGGCGGA CGTGATGGCG GTGCGCGAGC TCCTGGACGC CAACGGCGGC 
GCGCGCTGCA AGATCATCTC GCACGTGTCC ACGGCCAAGG CCATCGCCAA CTACGATGAG 
ATCCTGGATG CCACCGACGC CGTGCTCATC TCGCGCGCGT ACATGGGCAT GCGCATCCCC 
GCGGCCAAGG TGTCCCTGGC CCAGAAGTGG ATGACTGCCA AGGCCAACCT GAAGGGCAAG 
CCGTGCATCG TCAGCGCGCA GATCATGTAT AGCATGGTGG ACAGCCCCCG CCCCACCCGC 
GCCGAGATGA CGGACGTGGC CAACGCGCTG TACGACGGCG CCGACGCGCT GATGCTGCGC 
GAGGAGACCG CCGTGGGCTC GTTCGTGGAG CGCGTGGTGT CCATCACCGC CGACATCCTG 
CGCGACGCCG AGGTCGGAGT GGACACCTAC GCGCAGCTCA ACCAGCTGCG CAACTTCACG 
CCTAAGCCCA TGCCCACCCT GGAGTGCCTG TGTTCCTCGG CGGTCAAGGC GGCGGTGGAC 
ATGCGCGCGG CGCTGATTGC CGTCATCACC GACACCAGCG CGCCCGTGCG CGCCCTGGCC 
AAGTACCGCC CTGGCCAGGC CATTGTGGTG GTGACCACGC GCACGCACGT GGCGCGCCAG 
TGCAACATGA ACTACGGCTG CGTGCCGCTG CTGCTCCGCA GCAAGGACCG CGACGCGCCG 
CTGGACAAGA TCGTGAGCAA GATCGTGGAC TTTGCGCGCA CCCAGAAGCT GGCTGCATTC 
CAGGCCGGCG ACGACCAGGG CGACCAGCTC ATCATCGTGC AGGGCCCCTC CAACCGCACC 
TTTGCCACGG AGGAGGACCT GGCGGAGATG CAGTTCGTGA CGCGCATTAT GGGCGAGGAG 
GCGTCCGAGG TGTGCGCCAA GCTCGGCTAC ACTGGCGCTG ACACCATTGC CTACCGCTCC 
ACCAAGGTGG GCCTGGACCT CATCTGCGGC CCCACCGACA CGCGCACCCG CAAGACTAAG 
GTGGTGTGCA CGCTGGGCCC CAAGTGCTGG AGCAAGGAGG GCCTGGAGAT GCTGGTGGAC 
ACCGGCATCA ACGTGGCCAG GTTCAATTTC AGCCACGGCA GCCACGCCGA CCACCAGGAG 
GTGCTGGACC GCCTGCGCCT GGTGCTGGAC GAGCGCGGCG CGCGGCACCG TGTGGCGCTG 
CTGCTGGACA CCAAGGGCCC CGAGGTGCGC ACCGCCATGC TCAAGGACGG CAAGGACATC 
AACCTGGAGG CGGGCCAGGA GCTGACGGTG GTGGCGGTAG GCGACAAGTA CACCACCTGG 
GAGGGCTTCA AGGACGAGGC GACGGGCGAG ACCAGGATTG GCCTCAGCTA CCAGCACCTG 
TGCCGCGATG TCAAGGTCGG CGGCATGATC AAGATCGGTG ACGGCCTCAT CACCCTGGAG 
GACGGTGGCG GTGGCATTGT GCACTTGCAG GTGCTGGAGA TTCTGTCGCC CACGGAGCTG 
AGGGTGCGCG CGCTGAACAG CAAGGCGCTG GGCCAGCGCA AGAACGTCAA CCTGCCGGGT 
GTGCACGTGG ACATGCCTGT GCTGGGCCCC AACGACATCA ACGACGTGCA GAACTTCGCC 
GCCAAGAACA ACATGGACTT TGTGGCGGCG TCCTTCGTGC AGAGCGCCGA TGACGTGCGC 
TTCATCCGCA AGGTGCTGGA CGAGGCGGGC GGCAAGAACG TGCGCATCAT CTCCAAGATC 
GAGTCCACCG CCGGCCTGAT CAACTACGAC GCCATCCTGG CTGAGAGCGA CGGCATTATG 
GTGGCACGTG GCGACCTGGC CATGGAGATT CCCTCGGAGA AGGTGGCTCT GGCCCAGAAG 
ATGATGATCA CCAAGGCCAA CATTGCGGGC AAGTTCGTCA TCACCGCCAC CCAGATGCTG 
GAGTCCATGA TTAAATCGCC GCTGCCCACT CGTGCGGAGA TGACGGACGT GGCCAACGCC 
GTGTTCGATG GCACCGACGC CGTCATGCTC AGCGGCGAGA CCGCCAACGG CGACTTCCCC 
CAGCTGGCGC TGGCGACCAT GTGCGCCATC TCCGCCAACG CAGAGGTGGG CACCAACTAC 
CCGCAGGTGT ACGACTTCAT CCGCGACTTC TCTGCGCGCC CCATGTCCAC CGCCGAGGCG 
GTGCTGGGCT GCGCCGCCAA GAACGTGCTG GACGTGGACG CGGCGCTCAT CCTGGTCATC 
AGCTCCACCG GTGCCAGTGC CCGCCTGGTG GCCAAGTACC GCCCGCGCGT GCCGGTGCTG 
CTCATCACCG ACTCCATGGC CGCCGCGCGC TCCGTGGCGC CGCTCTTTGG TGTGTACGTC 
AGCATCGTGG ACGAGCTGCC GCCCTCGCGC TTCGACCTGG AGTTTGAGGT GCTGCTGGAG 
GAGGCGACCC TGTTCGCCAT GGAGGCCGGC CTGTGCCCGC CCGGCAAGGA GGTGGTGGTG 
GTGCACGGCT GCAACACGGC GGACGCGGAG GACGGGCTGC CCATGGTGGA GATCCAGGTG 
GCGGCGGGCG GCGTGCCGTC GCGCCAGACC AGTGAGGCCA TCAGCGAGAC CGTCACGCCC 
AAGCCCAGCC TGCCGCAGCC CAAGGCCAAG CGCACGGGGC CCAGCCCCTT CGCGCCGGCC 
GTGCCGCCCA GCCCCGCCAC CACCGCCTTC CAGCACGCCT TTTACCTGGG CGGCGCGGGC 
ATTCACCACG TCACGTCTGG CGTGACGCCC TTCGGCAGCC TGGCAGCCCT GGCCCCGTCC 
GAGGCGCCGC CCTCGCCCAA GCAGCAGCCC CACGCCACCA ACAGGCTCGT CGCCGACGTG 
GTGGAGGAGG GCGCCTCCAG CGGCGCTTCA GCCGCCGCCG CCCTGCGCGA CAACCTGCCC 
GCCATCCCCG CCGCCGCCAA GCCCGCCAGC AACGTGGCCT CGCTCACGCC GTCGCCGGCG 
CCCACGGCCT TGCCCAAGGC CCTCACCGCG GCCGTGATGG CGGCCAAGAC GCTGAGCCTG 
CGCACCACCG CCATCAGCCT GGTGGACATC CTGGACCACA GCCTGCCGGC CGGCCGCAAG 
ACCAAGATCG TGTGCACGCT GGGCCCCAGC TGCTGGTCCG AGGAGGGCCT CAACGGCATC 
ATCAACGCGG GCATGGACGT GGCGCGCTTC AACTTCAGCC ATGGCACCCA CCAGGCGCAC 
CAGGAGGTGC TGGACCGCCT GCGCAAGGTG GAGAAGGCCG GCACCAAGCG AATCGCCTAC 
CTGCTGGACA CCAAGGGCCC CGAGGTGCGC ACCGCCATGC TGCGCGAGGG CAAGGACATC 
AAGCTGGACG CCAACCAGGA GGTCACGGTG GTGGCGGTGG GAGACGACTA CGACAAGTGG 
GAGGGCTACA AGGACGAGAA GACGGGCGAG ACCAAGATCG GCATCAGCTA CGCCAAGCTG 
TGCCAGAGCG TGCGCGTGGG CAGCCGCATC CTGGTGGCGG ACGGCTCGCT GACCATTGAG 
GTGCTGGAGA TCACGTCGGC CACGGAGCTG CGCGGCCGCT GCCTCAACAC CAAGGTGCTG 
GGCCAGCGCA AGAACGTCAA CCTGCCCGGC GTGCACGTGG ACATCCCCGT GCTGACGCCC 
AAGGACATCG AGGACCTGCA GAAGTTCTGT GTGGGCAACG ACATGGACTT CGTGGCGGCG 
TCCTTCGTGC AGAGCGCCGA CGACGTGCGC CTCATCCGCC GCGTCCTTGA CGAGGCGGGC 
GGCTACAACG TGCAGATCAT CTCCAAGATT GAGAACGAGG CGGGCCTCAA GTATTTTGAC 
GGCATCCTGG CTGAGAGCGA CGGCATCATG GTGGCGCGCG GCGACCTGGC CATGGAGATT 
CCCTCGGAGA AGGTGGCTCT GGCCCAGAAG ATGATGATCA CCAAGGCCAA CATTGCCGGC 
AAGTTCGTCA TCACCGCCAC CCAGATGCTG GAGTCCATGA CCAGCGCGCC GCTGCCCACT 
CGTGCGGAGA TGACGGACGT GGCCAACGCC GTGTTCGATG GCACCGACGC CGTCATGCTC 
AGCGGCGAGA CCGCCAACGG CAAGTTCCCG CGCCCGGCGG TGGAGACCAT GGCCGCCATC 
GTGGCCAACG CCGAGAACGG CACCGCCTAC GTGTCCACGC AGTGGTTCAT CCGCGACCAC 
ACGCCCAAGC CGTTCGGCAT CACGGAGGCC ACCGGCGTGT CGTGCGTGGC TGCGTCCGTG 
GACTGCAACG CGCAGCTGAT CATCACGCTC ACCAGCGGCG GCTACACCAG CCGCTTCGTG 
TCCAAGTACC GCCCCGCCGT GCCGCAGATT GTGGTGACGG ACTCGGTGCA CGTGGCCAAC 
CAGGCCAGTG TGCACTTCGG CCAGTACGCG CTGCTGGTGG GCGACATCGA CTTCAGCACG 
TGGCCGGCGG ACCGCAGCCT GACCGCCATG GTGGACGAGC TGGTGGCGCA GGCGGCGCTG 
TTCGCGCAGG ACAAGGGGCT GTGGGCCGGC CAGGGCACAG CCATCCTGCT GCACGGCCGC 
GACGACCTGT CGGCGGACAG CCAGCCCGTG CTGCGCGCCG TGGACCTGGC CGACCTCGTG 
GGCGCCGCGC CCGCCGAGCT CACCGCCGCC AGCAGCAAGC TGTCGGCGGG CTCGGGCCCC 
GGCCGCGCCG GCTCGCGTGA GGTAGGGCTG GGCCGCACGT CCGCCGGCCT GGCGGGCACG 
TCCGTGAACC GCGCCTTCAG CCTGGTGGTG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The Chlamydomonas genome reveals the evolution of key animal and plant functions.
Science (New York, N.Y.)318(5848)245-50(2007 Oct)
Merchant SS,Prochnik SE,Vallon O,Harris EH,Karpowicz SJ,Witman GB,Terry A,Salamov A,Fritz-Laylin LK,Mar?chal-Drouard L,Marshall WF,Qu LH,Nelson DR,Sanderfoot AA,Spalding MH,Kapitonov VV,Ren Q,Ferris P,Lindquist E,Shapiro H,Lucas SM,Grimwood J,Schmutz J,Cardol P,Cerutti H,Chanfreau G,Chen CL,Cognat V,Croft MT,Dent R,Dutcher S,Fern?ndez E,Fukuzawa H,Gonz?lez-Ballester D,Gonz?lez-Halphen D,Hallmann A,Hanikenne M,Hippler M,Inwood W,Jabbari K,Kalanon M,Kuras R,Lefebvre PA,Lemaire SD,Lobanov AV,Lohr M,Manuell A,Meier I,Mets L,Mittag M,Mittelmeier T,Moroney JV,Moseley J,Napoli C,Nedelcu AM,Niyogi K,Novoselov SV,Paulsen IT,Pazour G,Purton S,Ral JP,Ria?o-Pach?n DM,Riekhof W,Rymarquis L,Schroda M,Stern D,Umen J,Willows R,Wilson N,Zimmer SL,Allmer J,Balk J,Bisova K,Chen CJ,Elias M,Gendler K,Hauser C,Lamb MR,Ledford H,Long JC,Minagawa J,Page MD,Pan J,Pootakham W,Roje S,Rose A,Stahlberg E,Terauchi AM,Yang P,Ball S,Bowler C,Dieckmann CL,Gladyshev VN,Green P,Jorgensen R,Mayfield S,Mueller-Roeber B,Rajamani S,Sayre RT,Brokstein P,Dubchak I,Goodstein D,Hornick L,Huang YW,Jhaveri J,Luo Y,Mart?nez D,Ngau WC,Otillar B,Poliakov A,Porter A,Szajkowski L,Werner G,Zhou K,Grigoriev IV,Rokhsar DS,Grossman AR

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PYK4a cDNA ORF clone, Chlamydomonas reinhardtii

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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