CcCYP12B3 cDNA ORF clone, Ceratitis capitata(Mediterranean fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	CcCYP12B3
Entrez Gene ID	101459565
Full Name	cytochrome P450 12B3
Synonyms	cyp12
Gene Type	protein-coding
Organism	Ceratitis capitata(Mediterranean fruit fly)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001319831.1	NP_001306760.1	cytochrome P450 12B3

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

A genomic perspective to assessing quality of mass-reared SIT flies used in Mediterranean fruit fly (Ceratitis capitata) eradication in California.
Calla B, Hall B, Hou S, Geib SM
BMC genomics1598(2014 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CcCYP12B3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CcCYP12B3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCe76186	NM_001319831.1 Latest version!	Ceratitis capitata cytochrome P450 12B3 (CcCYP12B3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CcCYP12B3 ( NM_001319831.1 ) cDNA ORF clone, Ceratitis capitata(Mediterranean fruit fly) -> NP_001306760.1 Ceratitis capitata cytochrome P450 12B3 (CcCYP12B3), mRNA.

Price & Availability↑

CloneID	OCe76186
Clone ID Related Accession (Same CDS sequence)	NM_001319831.1
Accession Version	NM_001319831.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1611bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-22
Organism	Ceratitis capitata(Mediterranean fruit fly)
Product	cytochrome P450 12B3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AOHK02000687.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## CDS exon combination :: GAMC01013498.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMN02055688, SAMN02055689 [ECO:0000348] ##Evidence-Data-END##

ATGATGAGAA CGAACTTTAC GCTTTTGACT ACCGCCGCTG GTAGAAAAAT AACAAAAAAT 
GCTTTAGATC AGCTGTTAGT GCGTCGGATA ACAAATAGTT TGCCATGCGC TCAACATGTT 
ATGGTTGATT ACTTCGAAGG AAAAGAGGAA CCACGCACCG ATGCTGATTG GTTGAATGCC 
AAGGATTACA AGGAAATTCC TGGACCCAGT AATTTTGAAT TAATAAGAGG TTATTTTCCA 
GGAGGAGCAT TATACAAAAC GACTATCAAA GAAATGGAAG TACATTTTCG TAAAAAATAT 
GGCGATATTA TTCGATTTAA CGGCACTGTG GGCAAAACAG ATATACTGTT CACCTTTAAC 
CCAGCTGATT TCGAAACAGT CTTTCGCAAT GAAACCATTT GGCCAGTACG TGTGGGTTTT 
CAGAGTTTGG CCTATTATCG GCTGGAAAAG CGTCCGCATA TTTTTCACGG TATTGACGGT 
TTGCTTACTT CGCAAGGACG TGCTTGGGGC TATATGCGTA ATAAAGTTAA TCCGGTGATG 
ATGAAAGTGC AAAATATTAG GCCAAACTTG CCAGCAGTCG ATGGTATTGC TCAGGAGTTC 
ATAGAGCGAC TCGATGCAAT ACTAGATCCT CACACTGGCA GATTATCGAC TGATTTCAGC 
GAAGAGGCTA AAATGTGGGC TTTCGAAACT GTAGCACTTG TGGCGCTCAA TACCCGCCTG 
GGGCTGATGC CGCGCGAAAA ATCCAATGCT ATGGCGTTAA GGCTATCCGA GAGCATAGCT 
GAATTTTTTA GTCTATGCTA TTACTATGAG GTGATCCCAC CCTTTTGGAG ATATTTCGAA 
ACAGCAGGAT TTAAGAAGCT CATAAAAGCT TACGATTCAA TTACTGAAAT CACAACACAT 
TACATTGAAA AAGCAATGGA GCAGTTCGAT CAAAGCGCCG AAGGGAAGAG CGTGCTGGAG 
AAGTTGTATC GCATAGATAA GAATGTTGCG GTGGTTATGG CCATGGATAT GTTGATGGCT 
GGCATGGATA CGACTTCCTC TGCATTGATT TCCACGCTAT ACTTCCTCAG CATAAACCCG 
GAAAAGCAAG AAGAATTACG CAAGGAATTA ATAGCGCTGC TGCCCGAACC AAATATGCCC 
TTAACTGAGG AGAACACCAA AAATATGCCC TATTTACGTG GCTGCATTAA GGAGACGCTA 
CGCTTTAGAC CGATTGCCAA TGGAAACTTC CGTATCGCCG GCCGTGATCT CGTGCTCTCC 
GGTTATAGGG TACCCAAAGG TGTTGGTGTG TATATGGCAA CCATGACACT CTCAAACAGC 
GATGAATACT TCGAACGTAG TGCTGAATTT TTGCCAGAAC GTTGGATCAA ATCGGCGAAA 
GCCACTTGCC CAGTCTCCCA AAAGCACAAT CCATTCGTTT ACTTGCCATT TGGTTTTGGG 
CCACGCACTT GTATTGGCAA ACGTCTGGCG GAGATGGAAT TGGAAACGAT TTTGGTGCGA 
ATATTACGTA GTTATCGTGT CAGTTGGGTG GGTGAGAAAC CATTGGAATA TATTAACAAC 
TTGATTTTGC AACCGGCTGG GGAAATGAGC TTTAAATTTG AAAAATTGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001306760.1
CDS	141..1751
Translation	MMRTNFTLLTTAAGRKITKNALDQLLVRRITNSLPCAQHVMVDYFEGKEEPRTDADWLNAKDYKEIPGPSNFELIRGYFPGGALYKTTIKEMEVHFRKKYGDIIRFNGTVGKTDILFTFNPADFETVFRNETIWPVRVGFQSLAYYRLEKRPHIFHGIDGLLTSQGRAWGYMRNKVNPVMMKVQNIRPNLPAVDGIAQEFIERLDAILDPHTGRLSTDFSEEAKMWAFETVALVALNTRLGLMPREKSNAMALRLSESIAEFFSLCYYYEVIPPFWRYFETAGFKKLIKAYDSITEITTHYIEKAMEQFDQSAEGKSVLEKLYRIDKNVAVVMAMDMLMAGMDTTSSALISTLYFLSINPEKQEELRKELIALLPEPNMPLTEENTKNMPYLRGCIKETLRFRPIANGNFRIAGRDLVLSGYRVPKGVGVYMATMTLSNSDEYFERSAEFLPERWIKSAKATCPVSQKHNPFVYLPFGFGPRTCIGKRLAEMELETILVRILRSYRVSWVGEKPLEYINNLILQPAGEMSFKFEKL

Target ORF information:

RefSeq Version	NM_001319831.1
Organism	Ceratitis capitata(Mediterranean fruit fly)
Definition	Ceratitis capitata cytochrome P450 12B3 (CcCYP12B3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001319831.1

ORF Insert Sequence:

ATGATGAGAA CGAACTTTAC GCTTTTGACT ACCGCCGCTG GTAGAAAAAT AACAAAAAAT 
GCTTTAGATC AGCTGTTAGT GCGTCGGATA ACAAATAGTT TGCCATGCGC TCAACATGTT 
ATGGTTGATT ACTTCGAAGG AAAAGAGGAA CCACGCACCG ATGCTGATTG GTTGAATGCC 
AAGGATTACA AGGAAATTCC TGGACCCAGT AATTTTGAAT TAATAAGAGG TTATTTTCCA 
GGAGGAGCAT TATACAAAAC GACTATCAAA GAAATGGAAG TACATTTTCG TAAAAAATAT 
GGCGATATTA TTCGATTTAA CGGCACTGTG GGCAAAACAG ATATACTGTT CACCTTTAAC 
CCAGCTGATT TCGAAACAGT CTTTCGCAAT GAAACCATTT GGCCAGTACG TGTGGGTTTT 
CAGAGTTTGG CCTATTATCG GCTGGAAAAG CGTCCGCATA TTTTTCACGG TATTGACGGT 
TTGCTTACTT CGCAAGGACG TGCTTGGGGC TATATGCGTA ATAAAGTTAA TCCGGTGATG 
ATGAAAGTGC AAAATATTAG GCCAAACTTG CCAGCAGTCG ATGGTATTGC TCAGGAGTTC 
ATAGAGCGAC TCGATGCAAT ACTAGATCCT CACACTGGCA GATTATCGAC TGATTTCAGC 
GAAGAGGCTA AAATGTGGGC TTTCGAAACT GTAGCACTTG TGGCGCTCAA TACCCGCCTG 
GGGCTGATGC CGCGCGAAAA ATCCAATGCT ATGGCGTTAA GGCTATCCGA GAGCATAGCT 
GAATTTTTTA GTCTATGCTA TTACTATGAG GTGATCCCAC CCTTTTGGAG ATATTTCGAA 
ACAGCAGGAT TTAAGAAGCT CATAAAAGCT TACGATTCAA TTACTGAAAT CACAACACAT 
TACATTGAAA AAGCAATGGA GCAGTTCGAT CAAAGCGCCG AAGGGAAGAG CGTGCTGGAG 
AAGTTGTATC GCATAGATAA GAATGTTGCG GTGGTTATGG CCATGGATAT GTTGATGGCT 
GGCATGGATA CGACTTCCTC TGCATTGATT TCCACGCTAT ACTTCCTCAG CATAAACCCG 
GAAAAGCAAG AAGAATTACG CAAGGAATTA ATAGCGCTGC TGCCCGAACC AAATATGCCC 
TTAACTGAGG AGAACACCAA AAATATGCCC TATTTACGTG GCTGCATTAA GGAGACGCTA 
CGCTTTAGAC CGATTGCCAA TGGAAACTTC CGTATCGCCG GCCGTGATCT CGTGCTCTCC 
GGTTATAGGG TACCCAAAGG TGTTGGTGTG TATATGGCAA CCATGACACT CTCAAACAGC 
GATGAATACT TCGAACGTAG TGCTGAATTT TTGCCAGAAC GTTGGATCAA ATCGGCGAAA 
GCCACTTGCC CAGTCTCCCA AAAGCACAAT CCATTCGTTT ACTTGCCATT TGGTTTTGGG 
CCACGCACTT GTATTGGCAA ACGTCTGGCG GAGATGGAAT TGGAAACGAT TTTGGTGCGA 
ATATTACGTA GTTATCGTGT CAGTTGGGTG GGTGAGAAAC CATTGGAATA TATTAACAAC 
TTGATTTTGC AACCGGCTGG GGAAATGAGC TTTAAATTTG AAAAATTGTA A