VPS35 cDNA ORF clone, Cariama cristata(Red-legged seriema)

Gene
Clones
gRNAs

Summary

Gene Symbol	VPS35
Entrez Gene ID	104155194
Full Name	VPS35, retromer complex component
Gene Type	protein-coding
Organism	Cariama cristata(Red-legged seriema)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009710526.1	XP_009708828.1	vacuolar protein sorting-associated protein 35

Pathways from BioSystems
Gene Ontology

The following VPS35 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the VPS35 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCa454036	XM_009710526.1 Latest version!	Cariama cristata vacuolar protein sorting 35 homolog (S. cerevisiae) (VPS35), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

VPS35 ( XM_009710526.1 ) cDNA ORF clone, Cariama cristata(Red-legged seriema) -> XP_009708828.1 Cariama cristata vacuolar protein sorting 35 homolog (S. cerevisiae) (VPS35), mRNA.

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CloneID	OCa454036
Clone ID Related Accession (Same CDS sequence)	XM_009710526.1
Accession Version	XM_009710526.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2304bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-10-20
Organism	Cariama cristata(Red-legged seriema)
Product	vacuolar protein sorting-associated protein 35
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_009593435.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Cariama cristata Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAGAT GCTTGGACAA AAACAAGCTC ATGGATGCTC TGAAACATGC TTCCAACATG 
CTTGGAGAGC TGCGGACTTC TATGTTATCT CCAAAGAGCT ATTATGAGTT ATACATGGCA 
ATTTCTGATG AGCTACACTA CTTGGAAGTT TACCTGACAG ATGAATTTGC CAAAGGAAGG 
AAAGTGGCAG ACCTTTATGA GCTAGTACAG TATGCTGGAA ATATTATTCC AAGACTGTAT 
CTCCTGATAA CAGTAGGTGT TGTCTATGTC AAGTCATTTC CACAGTCCAG GAAAGATATT 
TTGAAAGACC TGGTGGAGAT GTGCCGTGGA GTACAACATC CTCTTAGAGG CCTCTTTCTT 
AGAAACTATC TCCTGCAGTG TACCAGGAAT ATCTTACCAG ATGAAGGCGA GCAAGCAGAT 
GAAGAAACCA CTGGAGACAT CAGTGATTCA ATGGATTTTG TGCTGCTGAA CTTTGCTGAG 
ATGAACAAAC TTTGGGTGCG AATGCAACAC CAGGGCCATA GTCGGGACAG AGAGAAAAGG 
GAGCGAGAAA GGCAAGAACT GAGAATCCTA GTAGGAACAA ACCTGGTTCG CCTCAGTCAG 
TTGGAAGGTG TTAATGTGGA GCGTTATAAG CAGATTGTTC TGCCTGGAAT ATTGGAGCAA 
GTTGTAAACT GCAGAGATGC TTTGGCACAG GAGTACCTCA TGGAGTGCAT CATACAGGTG 
TTCCCAGATG AATTTCACCT CCAAACCCTG AACCCATTTC TCAGAGCCTG TGCTGAGCTG 
CACCAAAATG TGAATGTGAA AAATATAATT ATTGCTTTAA TTGACAGGTT AGCTTTATTT 
GCACATCGTG AAGATGGACC TGGTATCCCA GCAGATATCA AGCTGTTTGA CATCTTTTCA 
CAACAGGTGG CTACTGTTAT ACAGTCTCGT CAGGACATGC CCTCAGAGGA TGTGGTATCT 
TTGCAAGTTT CTCTTATTAA CTTGGCTATG AAATGCTACC CGGACCGTGT TGACTATGTT 
GATAAGGTGT TGGAGACAAC TGTGGAAATA TTCAATAAAC TTAATCTGGA ACATATTGCA 
ACAAGCAGTG CTGTTTCAAA GGAGCTGACT AGACTTTTGA AAATCCCTGT TGATACTTAC 
AACAATATCC TGACAGTTCT GAAACTAAAA CATTTTCACC CACTCTTTGA ATACTTTGAT 
TACGAGTCCA GGAAGAGCAT GAGTTGTTAT GTGCTTAGTA ATGTATTGGA TTATAACACA 
GAAATTGTGT CTCAAGAACA GGTTGATGCT ATCATGAATT TGGTATCCAC GCTGATCCAG 
GATCAGCCGG ATCAGCCTGC TGAAGATCCT GATCCTGAGG ACTTTGCTGA TGAGCAGAGT 
CTTGTGGGAA GATTTATTCA TCTTTTGCGT TCCGATGACC CTGACCAACA GTATCTGATC 
CTAAACACTG CCCGGAAACA CTTTGGTGCA GGTGGGAACC AACGTATTCG TTTTACACTG 
CCACCATTGG TTTTTGCAGC GTACCAGCTT GCTTTTCGCT ACAAAGACAA CTCTAAAGTG 
GACGACAAAT GGGAAAAGAA GTGCCAGAAG ATCTTCTCGT TTGCTCATCA GACCATCAGT 
GCCCTGATCA AAGCAGAACT GGCAGAGCTG CCTCTCCGTC TCTTCCTACA AGGAGCACTG 
GCTGCAGGAG AGATTGGCTT TGAGAATCAT GAAACTGTGG CCTATGAATT CATGTCCCAG 
GCCTTCTCTC TGTATGAAGA TGAAATCAGT GATTCAAAAG CACAACTGGC TGCAATCACC 
TTGATAATTG GGACATTTGA GAGGATGAAG TGCTTCAGTG AGGAGAACCA TGAGCCTTTG 
AGGACTCAGT GTGCACTGGC AGCCTCTAAG CTCCTTAAGA AGCCAGACCA ATGCCGGGCT 
GTGAGCACTT GTGCCCATCT GTTTTGGTCT GGACGAAATA CTGACAAGAA TGGAGAGGAG 
CTTCATGGAG GAAAAAGAGT GATGGAATGC CTAAAGAAGG CTCTGAAGAT AGCAAATCAG 
TGCATGGACC CTTCTCTGCA AGTCCAACTT TTCATAGAAA TTCTGAATAG ATATATCTAT 
TTTTATGAAA AAGAAAATGA GGCGGTGACA ATTCAGGTTT TGAATCAGCT TATACAGAAG 
ATAAGAGAAG ATCTCCCAAA CCTTGAGTCT ACTGAGGAAA CGGAACAAAT TAACAAACAC 
TTTCACAACA CACTGGAGCA CTTGCGTCTG AGGAGGGAAT CACCAGAATC TGAGGGGCCA 
ATTTACGAAG GTCTTGTTCT TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009708828.1
CDS	80..2383
Translation	MKRCLDKNKLMDALKHASNMLGELRTSMLSPKSYYELYMAISDELHYLEVYLTDEFAKGRKVADLYELVQYAGNIIPRLYLLITVGVVYVKSFPQSRKDILKDLVEMCRGVQHPLRGLFLRNYLLQCTRNILPDEGEQADEETTGDISDSMDFVLLNFAEMNKLWVRMQHQGHSRDREKRERERQELRILVGTNLVRLSQLEGVNVERYKQIVLPGILEQVVNCRDALAQEYLMECIIQVFPDEFHLQTLNPFLRACAELHQNVNVKNIIIALIDRLALFAHREDGPGIPADIKLFDIFSQQVATVIQSRQDMPSEDVVSLQVSLINLAMKCYPDRVDYVDKVLETTVEIFNKLNLEHIATSSAVSKELTRLLKIPVDTYNNILTVLKLKHFHPLFEYFDYESRKSMSCYVLSNVLDYNTEIVSQEQVDAIMNLVSTLIQDQPDQPAEDPDPEDFADEQSLVGRFIHLLRSDDPDQQYLILNTARKHFGAGGNQRIRFTLPPLVFAAYQLAFRYKDNSKVDDKWEKKCQKIFSFAHQTISALIKAELAELPLRLFLQGALAAGEIGFENHETVAYEFMSQAFSLYEDEISDSKAQLAAITLIIGTFERMKCFSEENHEPLRTQCALAASKLLKKPDQCRAVSTCAHLFWSGRNTDKNGEELHGGKRVMECLKKALKIANQCMDPSLQVQLFIEILNRYIYFYEKENEAVTIQVLNQLIQKIREDLPNLESTEETEQINKHFHNTLEHLRLRRESPESEGPIYEGLVL

Target ORF information:

RefSeq Version	XM_009710526.1
Organism	Cariama cristata(Red-legged seriema)
Definition	Cariama cristata vacuolar protein sorting 35 homolog (S. cerevisiae) (VPS35), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009710526.1

ORF Insert Sequence:

ATGAAGAGAT GCTTGGACAA AAACAAGCTC ATGGATGCTC TGAAACATGC TTCCAACATG 
CTTGGAGAGC TGCGGACTTC TATGTTATCT CCAAAGAGCT ATTATGAGTT ATACATGGCA 
ATTTCTGATG AGCTACACTA CTTGGAAGTT TACCTGACAG ATGAATTTGC CAAAGGAAGG 
AAAGTGGCAG ACCTTTATGA GCTAGTACAG TATGCTGGAA ATATTATTCC AAGACTGTAT 
CTCCTGATAA CAGTAGGTGT TGTCTATGTC AAGTCATTTC CACAGTCCAG GAAAGATATT 
TTGAAAGACC TGGTGGAGAT GTGCCGTGGA GTACAACATC CTCTTAGAGG CCTCTTTCTT 
AGAAACTATC TCCTGCAGTG TACCAGGAAT ATCTTACCAG ATGAAGGCGA GCAAGCAGAT 
GAAGAAACCA CTGGAGACAT CAGTGATTCA ATGGATTTTG TGCTGCTGAA CTTTGCTGAG 
ATGAACAAAC TTTGGGTGCG AATGCAACAC CAGGGCCATA GTCGGGACAG AGAGAAAAGG 
GAGCGAGAAA GGCAAGAACT GAGAATCCTA GTAGGAACAA ACCTGGTTCG CCTCAGTCAG 
TTGGAAGGTG TTAATGTGGA GCGTTATAAG CAGATTGTTC TGCCTGGAAT ATTGGAGCAA 
GTTGTAAACT GCAGAGATGC TTTGGCACAG GAGTACCTCA TGGAGTGCAT CATACAGGTG 
TTCCCAGATG AATTTCACCT CCAAACCCTG AACCCATTTC TCAGAGCCTG TGCTGAGCTG 
CACCAAAATG TGAATGTGAA AAATATAATT ATTGCTTTAA TTGACAGGTT AGCTTTATTT 
GCACATCGTG AAGATGGACC TGGTATCCCA GCAGATATCA AGCTGTTTGA CATCTTTTCA 
CAACAGGTGG CTACTGTTAT ACAGTCTCGT CAGGACATGC CCTCAGAGGA TGTGGTATCT 
TTGCAAGTTT CTCTTATTAA CTTGGCTATG AAATGCTACC CGGACCGTGT TGACTATGTT 
GATAAGGTGT TGGAGACAAC TGTGGAAATA TTCAATAAAC TTAATCTGGA ACATATTGCA 
ACAAGCAGTG CTGTTTCAAA GGAGCTGACT AGACTTTTGA AAATCCCTGT TGATACTTAC 
AACAATATCC TGACAGTTCT GAAACTAAAA CATTTTCACC CACTCTTTGA ATACTTTGAT 
TACGAGTCCA GGAAGAGCAT GAGTTGTTAT GTGCTTAGTA ATGTATTGGA TTATAACACA 
GAAATTGTGT CTCAAGAACA GGTTGATGCT ATCATGAATT TGGTATCCAC GCTGATCCAG 
GATCAGCCGG ATCAGCCTGC TGAAGATCCT GATCCTGAGG ACTTTGCTGA TGAGCAGAGT 
CTTGTGGGAA GATTTATTCA TCTTTTGCGT TCCGATGACC CTGACCAACA GTATCTGATC 
CTAAACACTG CCCGGAAACA CTTTGGTGCA GGTGGGAACC AACGTATTCG TTTTACACTG 
CCACCATTGG TTTTTGCAGC GTACCAGCTT GCTTTTCGCT ACAAAGACAA CTCTAAAGTG 
GACGACAAAT GGGAAAAGAA GTGCCAGAAG ATCTTCTCGT TTGCTCATCA GACCATCAGT 
GCCCTGATCA AAGCAGAACT GGCAGAGCTG CCTCTCCGTC TCTTCCTACA AGGAGCACTG 
GCTGCAGGAG AGATTGGCTT TGAGAATCAT GAAACTGTGG CCTATGAATT CATGTCCCAG 
GCCTTCTCTC TGTATGAAGA TGAAATCAGT GATTCAAAAG CACAACTGGC TGCAATCACC 
TTGATAATTG GGACATTTGA GAGGATGAAG TGCTTCAGTG AGGAGAACCA TGAGCCTTTG 
AGGACTCAGT GTGCACTGGC AGCCTCTAAG CTCCTTAAGA AGCCAGACCA ATGCCGGGCT 
GTGAGCACTT GTGCCCATCT GTTTTGGTCT GGACGAAATA CTGACAAGAA TGGAGAGGAG 
CTTCATGGAG GAAAAAGAGT GATGGAATGC CTAAAGAAGG CTCTGAAGAT AGCAAATCAG 
TGCATGGACC CTTCTCTGCA AGTCCAACTT TTCATAGAAA TTCTGAATAG ATATATCTAT 
TTTTATGAAA AAGAAAATGA GGCGGTGACA ATTCAGGTTT TGAATCAGCT TATACAGAAG 
ATAAGAGAAG ATCTCCCAAA CCTTGAGTCT ACTGAGGAAA CGGAACAAAT TAACAAACAC 
TTTCACAACA CACTGGAGCA CTTGCGTCTG AGGAGGGAAT CACCAGAATC TGAGGGGCCA 
ATTTACGAAG GTCTTGTTCT TTAG