SMG5 cDNA ORF clone, Canis lupus familiaris(Dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	SMG5
Entrez Gene ID	490414
Full Name	SMG5, nonsense mediated mRNA decay factor
General protein information	Preferred Names SMG5, nonsense mediated mRNA decay factor Names protein SMG5 smg-5 homolog, nonsense mediated mRNA decay factor
Gene Type	protein-coding
Organism	Canis lupus familiaris(Dog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_547535.7	XP_547535.3	protein SMG5 isoform X2
XM_014115317.1	XP_013970792.1	protein SMG5 isoform X2
XM_022421262.1	XP_022276970.1	protein SMG5 isoform X3
XM_022421261.1	XP_022276969.1	protein SMG5 isoform X1
XM_547535.6	XP_547535.3	protein SMG5 isoform X1
XM_014115318.1	XP_013970793.1	protein SMG5 isoform X3

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	SMG5	XP_513880.3
	SMG5	XP_002801899.1
Canis lupus familiaris (dog)	SMG5	XP_547535.3
Bos taurus (cattle)	SMG5	NP_001077148.1
Rattus norvegicus (Norway rat)	Smg5	XP_001059906.3
Gallus gallus (chicken)	SMG5	XP_423228.4
Xenopus tropicalis (tropical clawed frog)	smg5	NP_001072622.1
Homo sapiens (human)	SMG5	NP_056142.2
Mus musculus (house mouse)	Smg5	NP_839977.2

Gene Ontology

The following SMG5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SMG5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe37561	XM_014115317.1 Latest version!	Canis lupus familiaris SMG5 nonsense mediated mRNA decay factor (SMG5), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OCe37562	XM_014115318.1 Latest version!	Canis lupus familiaris SMG5 nonsense mediated mRNA decay factor (SMG5), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OCe09508	XM_547535.7 Latest version!	Canis lupus familiaris SMG5, nonsense mediated mRNA decay factor (SMG5), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OCe289688	XM_022421261.1 Latest version!	Canis lupus familiaris SMG5, nonsense mediated mRNA decay factor (SMG5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OCe289689	XM_022421262.1 Latest version!	Canis lupus familiaris SMG5, nonsense mediated mRNA decay factor (SMG5), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
View Old Accession Versions >>
OCe09508	XM_547535.6	Canis lupus familiaris SMG5 nonsense mediated mRNA decay factor (SMG5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SMG5 ( XM_014115317.1 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_013970792.1 Canis lupus familiaris SMG5 nonsense mediated mRNA decay factor (SMG5), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCe37561
Clone ID Related Accession (Same CDS sequence)	XM_014115317.1
Accession Version	XM_014115317.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2997bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	protein SMG5 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726067.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCCAAG GCCCCCCCCC AGGGGAGAGC AGCGAGCCCG AGGCGAAGGT CCTCCATACC 
AAGCGGCTTT ACCGGGCTGT GGTGGAGGCT GTGCATCGAC TCGACCTCAT CCTTTGCAAC 
AAAACTGCTT ATCAAGAAGT GTTTAAACCA GAGAACATTA GCCTGAGGAA CAAGCTGCGT 
GAGCTCTGCG TCAAGCTTAT GTTCCTGCAC CCAGTAGACT ATGGGAGAAA GGCTGAGGAG 
CTGCTGTGGA GAAAGGTGTA CTACGAAGTT ATTCAGCTTA TCAAGACGAA TAAGAAGCAC 
ATCCACAGCC GGAGCACCTT GGAATGTGCC TACAGGACGC ACCTGGTTGC TGGGATTGGC 
TTCTACCAGC ATCTGCTGCT CTACATCCAG TCCCACTACC AGCTGGAGCT TCAGTGCTGC 
ATCGACTGGA CCCATGTTAC TGATCCCCTC ATAGGATGCA AGAAGCCAGT GTCTGCATCA 
GGGAAGGAGA TGGACTGGGC ACAGATGGCC TGCCACCGAT GTCTCATGTA CCTGGGGGAT 
TTGTCACGAT ATCAGAATGA ATTAGCTGGC GTAGACACTG AGCTGCTAGC TGAGAGATTT 
TACTACCAAG CCCTGTCTGT AGCTCCCCAG ATCGGAATGC CCTTTAACCA GCTGGGCACC 
CTTGCAGGCA GCAAGTACTA CAACGTGGAA GCCATGTACT GTTACCTGCG CTGCATCCAG 
TCGGAAGTGT CCTTTGAGGG GGCCTATGGG AATCTCAAGC GGCTGTATGA CAAGGCAGCC 
AAGATGTACC ACCAGCTGAA GAAGTGTGAG ACTCGGAAAC TCTCTCCCAG CAAGAAACGC 
TGTAAAGACA TCAAGAGGTT GCTGGTAAAC TTCATGTACC TTCAAAGCCT CCTGCAGCCC 
AGGAGCAGCC CGGTGGACTC AGAGCTGACC TCACTCTGCC AGTCAGTCCT GGAGGACTTC 
AATCTCTGCC TCTTCTACCT GCCATCCTCA CCCAGCCTCA GCCTGGCCAG TGAGGGTGAA 
GAGGACTATG AAAGTGGCTA TGCCTTCCTC CCAGACCTCC TCATCTTTCA GATGATCATC 
ATTTGTCTCA TGGGTGTGCA TAGCTTGAAG AGAGCAGGGT CCAAACAGTA CAGTGCAGCC 
ATTGCCTTTA CCCTGGCCCT CTTCTCCCAC CTCGTCAACC ATGTCAATAT ACGGCTGCAG 
GCTGAGCTGG AGGAGGATGA GCCCTTGGAG AAGGAGGAGG CACCGGGGCC TGAGCCCCCT 
CCTGCAGTAC CTCAAGCGAC TGAGGTCAGA AAGAGCCGGA AGTTCTCTCG CCTCTCCTGC 
CTCCGCCGCC GCCGCCACCC ACCCAAAGCT GGAGATGACA GTGACCTGAG TGAAGGCTTT 
GAATCAGACT CGAGCCATGA CTCAGCCAGG GCCAGTGAGG GCTCAGACAG TGGCTCCGAC 
AAGAGTCTCG AAGGTGGGGG AACAGCCTTT GATGCTGAGA CAGACTCAGA AATGAACAGC 
CAAGAGTCCC GGTCAGACCT GGAAGACATT GAGGATGAGG AGGGTACTCG GTCCCCAGCC 
CTGGAGCCCC CTCGGCCCAG GTCAGAGGCT CCCGAGTCTC TCAATGGCCC ACTGGGCCCC 
AGTGAGGCGA GCATTGCCAG CAATCTACAG GCCATGTCCA CGCAGATGTT CCAGACCAAG 
CGCTGCTTCC GACTGGCGCC CACCTTCAGC AACCTGCTCC TCCAGCCTTC CACTGAGCCC 
CACACTGTAG CCAACCGCAG GTCATGTGTC AATGGAGATG CAGACAAGCC TTCGGAACCA 
GCCTCCGAGG AGGGCTCCGA GTCGGAGGGC AGTGAATCTA GTGGGCACTC CTGTCGGAAT 
GAGCGCAGTG TCCAGGAGAA GCTGCAAGTC CTGGCAGCCG AAGGCCTGCT TCCTGCTGTG 
AAGGTCTTCC TGGACTGGCT GCGGGCCAAC CCTGACCTCA TCGTTGTGTG TGCACAGAGC 
TCTCAAAGTC TGTGGAATCG CCTGTCTGTG TTGCTGAACC TGTTGCCAGC TGCTGGTGAG 
CTCCAAGAGT CTGGCCTGGC CCTGTGTCCC GAGGTCCAGG ACCTACTTGA AGGTTGTGAA 
CTGCCTGACC TGCCCTCCAG TCTGCTGCTC CCAGAGGACA CGGCCCTTCG TAACCTGCCT 
CCCCTCCGGG CTGCCCATAG ACGCTTCAAC TTTGACACAG ACCGGCCCCT GCTTAACTCC 
TTAGAGGAGT CAGTCGTGCG CATCTGCTGC ATCCGCAGCT TTGGACACTT CATCGCCCAC 
CTGCAGGGCA GCATCCTGCA GTTCAGCGCA GAGGCTGGCA TCTTTGTCAG CATCGCCCAG 
TCTGAGCAGG AGGGCGTGCT GCAGCAGGCC CAGGCGCAGT TCCGCATGGC TCAGGAAGAG 
GCCCGTCGGA ACAGGCTGAT GAGAGACATG GCCCAGCTGC GACTTCAGCT CGAGGTCTCT 
CAGCTGGAAG GGAGCCTGCA GCAGCCCAAG GCCCAGTCAG CAATGTCTCC TTACCTCGTC 
CCGGACACCC AGGCCCTCTG CCACCACCTC CCGGTCATCC GCCAGCTGGC CACCAGCGGC 
CGCTTCATTG TCATCATCCC AAGGACAGTG ATTGATGGCC TGGATTTGCT GAAGAAGGAA 
CACCCAGGGG CCCGGGATGG GATCCGTTAC CTGGAGGCAG AGTTTAAGAA AGGAAACAGG 
TACATTCGCT GCCAGAAAGA AGTAGGAAAG AGCTTCGAGC GGCATAAGCT GAAGAGGCAG 
GATGCAGATG CCTGGACGCT CTATAAGATC CTAGACAGCT GCAAACAGTT GACTTTGGCC 
CAGGGGGCAG GCGAGGAGGA CCCAAGCGGC ATGGTGACCA TCGTCACAGG CTTTCCACTG 
GACAACCCCA GTGCGCTCTC AGGCCCCATG CAGGCAGCCC TGCAGGCCGC TACACATGCC 
AGTGTGGACA TCAAGAATGT TCTGGACTTC TACAAGCAGT GGAAGGAGAT TGGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013970792.1
CDS	173..3169
Translation	MSQGPPPGESSEPEAKVLHTKRLYRAVVEAVHRLDLILCNKTAYQEVFKPENISLRNKLRELCVKLMFLHPVDYGRKAEELLWRKVYYEVIQLIKTNKKHIHSRSTLECAYRTHLVAGIGFYQHLLLYIQSHYQLELQCCIDWTHVTDPLIGCKKPVSASGKEMDWAQMACHRCLMYLGDLSRYQNELAGVDTELLAERFYYQALSVAPQIGMPFNQLGTLAGSKYYNVEAMYCYLRCIQSEVSFEGAYGNLKRLYDKAAKMYHQLKKCETRKLSPSKKRCKDIKRLLVNFMYLQSLLQPRSSPVDSELTSLCQSVLEDFNLCLFYLPSSPSLSLASEGEEDYESGYAFLPDLLIFQMIIICLMGVHSLKRAGSKQYSAAIAFTLALFSHLVNHVNIRLQAELEEDEPLEKEEAPGPEPPPAVPQATEVRKSRKFSRLSCLRRRRHPPKAGDDSDLSEGFESDSSHDSARASEGSDSGSDKSLEGGGTAFDAETDSEMNSQESRSDLEDIEDEEGTRSPALEPPRPRSEAPESLNGPLGPSEASIASNLQAMSTQMFQTKRCFRLAPTFSNLLLQPSTEPHTVANRRSCVNGDADKPSEPASEEGSESEGSESSGHSCRNERSVQEKLQVLAAEGLLPAVKVFLDWLRANPDLIVVCAQSSQSLWNRLSVLLNLLPAAGELQESGLALCPEVQDLLEGCELPDLPSSLLLPEDTALRNLPPLRAAHRRFNFDTDRPLLNSLEESVVRICCIRSFGHFIAHLQGSILQFSAEAGIFVSIAQSEQEGVLQQAQAQFRMAQEEARRNRLMRDMAQLRLQLEVSQLEGSLQQPKAQSAMSPYLVPDTQALCHHLPVIRQLATSGRFIVIIPRTVIDGLDLLKKEHPGARDGIRYLEAEFKKGNRYIRCQKEVGKSFERHKLKRQDADAWTLYKILDSCKQLTLAQGAGEEDPSGMVTIVTGFPLDNPSALSGPMQAALQAATHASVDIKNVLDFYKQWKEIG

Target ORF information:

RefSeq Version	XM_014115317.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris SMG5 nonsense mediated mRNA decay factor (SMG5), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014115317.1

ORF Insert Sequence:

ATGAGCCAAG GCCCCCCCCC AGGGGAGAGC AGCGAGCCCG AGGCGAAGGT CCTCCATACC 
AAGCGGCTTT ACCGGGCTGT GGTGGAGGCT GTGCATCGAC TCGACCTCAT CCTTTGCAAC 
AAAACTGCTT ATCAAGAAGT GTTTAAACCA GAGAACATTA GCCTGAGGAA CAAGCTGCGT 
GAGCTCTGCG TCAAGCTTAT GTTCCTGCAC CCAGTAGACT ATGGGAGAAA GGCTGAGGAG 
CTGCTGTGGA GAAAGGTGTA CTACGAAGTT ATTCAGCTTA TCAAGACGAA TAAGAAGCAC 
ATCCACAGCC GGAGCACCTT GGAATGTGCC TACAGGACGC ACCTGGTTGC TGGGATTGGC 
TTCTACCAGC ATCTGCTGCT CTACATCCAG TCCCACTACC AGCTGGAGCT TCAGTGCTGC 
ATCGACTGGA CCCATGTTAC TGATCCCCTC ATAGGATGCA AGAAGCCAGT GTCTGCATCA 
GGGAAGGAGA TGGACTGGGC ACAGATGGCC TGCCACCGAT GTCTCATGTA CCTGGGGGAT 
TTGTCACGAT ATCAGAATGA ATTAGCTGGC GTAGACACTG AGCTGCTAGC TGAGAGATTT 
TACTACCAAG CCCTGTCTGT AGCTCCCCAG ATCGGAATGC CCTTTAACCA GCTGGGCACC 
CTTGCAGGCA GCAAGTACTA CAACGTGGAA GCCATGTACT GTTACCTGCG CTGCATCCAG 
TCGGAAGTGT CCTTTGAGGG GGCCTATGGG AATCTCAAGC GGCTGTATGA CAAGGCAGCC 
AAGATGTACC ACCAGCTGAA GAAGTGTGAG ACTCGGAAAC TCTCTCCCAG CAAGAAACGC 
TGTAAAGACA TCAAGAGGTT GCTGGTAAAC TTCATGTACC TTCAAAGCCT CCTGCAGCCC 
AGGAGCAGCC CGGTGGACTC AGAGCTGACC TCACTCTGCC AGTCAGTCCT GGAGGACTTC 
AATCTCTGCC TCTTCTACCT GCCATCCTCA CCCAGCCTCA GCCTGGCCAG TGAGGGTGAA 
GAGGACTATG AAAGTGGCTA TGCCTTCCTC CCAGACCTCC TCATCTTTCA GATGATCATC 
ATTTGTCTCA TGGGTGTGCA TAGCTTGAAG AGAGCAGGGT CCAAACAGTA CAGTGCAGCC 
ATTGCCTTTA CCCTGGCCCT CTTCTCCCAC CTCGTCAACC ATGTCAATAT ACGGCTGCAG 
GCTGAGCTGG AGGAGGATGA GCCCTTGGAG AAGGAGGAGG CACCGGGGCC TGAGCCCCCT 
CCTGCAGTAC CTCAAGCGAC TGAGGTCAGA AAGAGCCGGA AGTTCTCTCG CCTCTCCTGC 
CTCCGCCGCC GCCGCCACCC ACCCAAAGCT GGAGATGACA GTGACCTGAG TGAAGGCTTT 
GAATCAGACT CGAGCCATGA CTCAGCCAGG GCCAGTGAGG GCTCAGACAG TGGCTCCGAC 
AAGAGTCTCG AAGGTGGGGG AACAGCCTTT GATGCTGAGA CAGACTCAGA AATGAACAGC 
CAAGAGTCCC GGTCAGACCT GGAAGACATT GAGGATGAGG AGGGTACTCG GTCCCCAGCC 
CTGGAGCCCC CTCGGCCCAG GTCAGAGGCT CCCGAGTCTC TCAATGGCCC ACTGGGCCCC 
AGTGAGGCGA GCATTGCCAG CAATCTACAG GCCATGTCCA CGCAGATGTT CCAGACCAAG 
CGCTGCTTCC GACTGGCGCC CACCTTCAGC AACCTGCTCC TCCAGCCTTC CACTGAGCCC 
CACACTGTAG CCAACCGCAG GTCATGTGTC AATGGAGATG CAGACAAGCC TTCGGAACCA 
GCCTCCGAGG AGGGCTCCGA GTCGGAGGGC AGTGAATCTA GTGGGCACTC CTGTCGGAAT 
GAGCGCAGTG TCCAGGAGAA GCTGCAAGTC CTGGCAGCCG AAGGCCTGCT TCCTGCTGTG 
AAGGTCTTCC TGGACTGGCT GCGGGCCAAC CCTGACCTCA TCGTTGTGTG TGCACAGAGC 
TCTCAAAGTC TGTGGAATCG CCTGTCTGTG TTGCTGAACC TGTTGCCAGC TGCTGGTGAG 
CTCCAAGAGT CTGGCCTGGC CCTGTGTCCC GAGGTCCAGG ACCTACTTGA AGGTTGTGAA 
CTGCCTGACC TGCCCTCCAG TCTGCTGCTC CCAGAGGACA CGGCCCTTCG TAACCTGCCT 
CCCCTCCGGG CTGCCCATAG ACGCTTCAAC TTTGACACAG ACCGGCCCCT GCTTAACTCC 
TTAGAGGAGT CAGTCGTGCG CATCTGCTGC ATCCGCAGCT TTGGACACTT CATCGCCCAC 
CTGCAGGGCA GCATCCTGCA GTTCAGCGCA GAGGCTGGCA TCTTTGTCAG CATCGCCCAG 
TCTGAGCAGG AGGGCGTGCT GCAGCAGGCC CAGGCGCAGT TCCGCATGGC TCAGGAAGAG 
GCCCGTCGGA ACAGGCTGAT GAGAGACATG GCCCAGCTGC GACTTCAGCT CGAGGTCTCT 
CAGCTGGAAG GGAGCCTGCA GCAGCCCAAG GCCCAGTCAG CAATGTCTCC TTACCTCGTC 
CCGGACACCC AGGCCCTCTG CCACCACCTC CCGGTCATCC GCCAGCTGGC CACCAGCGGC 
CGCTTCATTG TCATCATCCC AAGGACAGTG ATTGATGGCC TGGATTTGCT GAAGAAGGAA 
CACCCAGGGG CCCGGGATGG GATCCGTTAC CTGGAGGCAG AGTTTAAGAA AGGAAACAGG 
TACATTCGCT GCCAGAAAGA AGTAGGAAAG AGCTTCGAGC GGCATAAGCT GAAGAGGCAG 
GATGCAGATG CCTGGACGCT CTATAAGATC CTAGACAGCT GCAAACAGTT GACTTTGGCC 
CAGGGGGCAG GCGAGGAGGA CCCAAGCGGC ATGGTGACCA TCGTCACAGG CTTTCCACTG 
GACAACCCCA GTGCGCTCTC AGGCCCCATG CAGGCAGCCC TGCAGGCCGC TACACATGCC 
AGTGTGGACA TCAAGAATGT TCTGGACTTC TACAAGCAGT GGAAGGAGAT TGGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMG5 ( XM_014115318.1 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_013970793.1 Canis lupus familiaris SMG5 nonsense mediated mRNA decay factor (SMG5), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OCe37562
Clone ID Related Accession (Same CDS sequence)	XM_014115318.1
Accession Version	XM_014115318.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2898bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	protein SMG5 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726067.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCCAAG GCCCCCCCCC AGGGGAGAGC AGCGAGCCCG AGGCGAAGGT CCTCCATACC 
AAGCGGCTTT ACCGGGCTGT GGTGGAGGCT GTGCATCGAC TCGACCTCAT CCTTTGCAAC 
AAAACTGCTT ATCAAGAAGT GTTTAAACCA GAGAACATTA GCCTGAGGAA CAAGCTGCGT 
GAGCTCTGCG TCAAGCTTAT GTTCCTGCAC CCAGTAGACT ATGGGAGAAA GGCTGAGGAG 
CTGCTGTGGA GAAAGGTGTA CTACGAAGTT ATTCAGCTTA TCAAGACGAA TAAGAAGCAC 
ATCCACAGCC GGAGCACCTT GGAATGTGCC TACAGGACGC ACCTGGTTGC TGGGATTGGC 
TTCTACCAGC ATCTGCTGCT CTACATCCAG TCCCACTACC AGCTGGAGCT TCAGTGCTGC 
ATCGACTGGA CCCATGTTAC TGATCCCCTC ATAGGATGCA AGAAGCCAGT GTCTGCATCA 
GGGAAGGAGA TGGACTGGGC ACAGATGGCC TGCCACCGAT GTCTCATGTA CCTGGGGGAT 
TTGTCACGAT ATCAGAATGA ATTAGCTGGC GTAGACACTG AGCTGCTAGC TGAGAGATTT 
TACTACCAAG CCCTGTCTGT AGCTCCCCAG ATCGGAATGC CCTTTAACCA GCTGGGCACC 
CTTGCAGGCA GCAAGTACTA CAACGTGGAA GCCATGTACT GTTACCTGCG CTGCATCCAG 
TCGGAAGTGT CCTTTGAGGG GGCCTATGGG AATCTCAAGC GGCTGTATGA CAAGGCAGCC 
AAGATGTACC ACCAGCTGAA GAAGTGTGAG ACTCGGAAAC TCTCTCCCAG CAAGAAACGC 
TGTAAAGACA TCAAGAGGTT GCTGGTAAAC TTCATGTACC TTCAAAGCCT CCTGCAGCCC 
AGGAGCAGCC CGGTGGACTC AGAGCTGACC TCACTCTGCC AGTCAGTCCT GGAGGACTTC 
AATCTCTGCC TCTTCTACCT GCCATCCTCA CCCAGCCTCA GCCTGGCCAG TGAGGGTGAA 
GAGGACTATG AAAGTGGCTA TGCCTTCCTC CCAGACCTCC TCATCTTTCA GATGATCATC 
ATTTGTCTCA TGGGTGTGCA TAGCTTGAAG AGAGCAGATG AGCCCTTGGA GAAGGAGGAG 
GCACCGGGGC CTGAGCCCCC TCCTGCAGTA CCTCAAGCGA CTGAGGTCAG AAAGAGCCGG 
AAGTTCTCTC GCCTCTCCTG CCTCCGCCGC CGCCGCCACC CACCCAAAGC TGGAGATGAC 
AGTGACCTGA GTGAAGGCTT TGAATCAGAC TCGAGCCATG ACTCAGCCAG GGCCAGTGAG 
GGCTCAGACA GTGGCTCCGA CAAGAGTCTC GAAGGTGGGG GAACAGCCTT TGATGCTGAG 
ACAGACTCAG AAATGAACAG CCAAGAGTCC CGGTCAGACC TGGAAGACAT TGAGGATGAG 
GAGGGTACTC GGTCCCCAGC CCTGGAGCCC CCTCGGCCCA GGTCAGAGGC TCCCGAGTCT 
CTCAATGGCC CACTGGGCCC CAGTGAGGCG AGCATTGCCA GCAATCTACA GGCCATGTCC 
ACGCAGATGT TCCAGACCAA GCGCTGCTTC CGACTGGCGC CCACCTTCAG CAACCTGCTC 
CTCCAGCCTT CCACTGAGCC CCACACTGTA GCCAACCGCA GGTCATGTGT CAATGGAGAT 
GCAGACAAGC CTTCGGAACC AGCCTCCGAG GAGGGCTCCG AGTCGGAGGG CAGTGAATCT 
AGTGGGCACT CCTGTCGGAA TGAGCGCAGT GTCCAGGAGA AGCTGCAAGT CCTGGCAGCC 
GAAGGCCTGC TTCCTGCTGT GAAGGTCTTC CTGGACTGGC TGCGGGCCAA CCCTGACCTC 
ATCGTTGTGT GTGCACAGAG CTCTCAAAGT CTGTGGAATC GCCTGTCTGT GTTGCTGAAC 
CTGTTGCCAG CTGCTGGTGA GCTCCAAGAG TCTGGCCTGG CCCTGTGTCC CGAGGTCCAG 
GACCTACTTG AAGGTTGTGA ACTGCCTGAC CTGCCCTCCA GTCTGCTGCT CCCAGAGGAC 
ACGGCCCTTC GTAACCTGCC TCCCCTCCGG GCTGCCCATA GACGCTTCAA CTTTGACACA 
GACCGGCCCC TGCTTAACTC CTTAGAGGAG TCAGTCGTGC GCATCTGCTG CATCCGCAGC 
TTTGGACACT TCATCGCCCA CCTGCAGGGC AGCATCCTGC AGTTCAGCGC AGAGGCTGGC 
ATCTTTGTCA GCATCGCCCA GTCTGAGCAG GAGGGCGTGC TGCAGCAGGC CCAGGCGCAG 
TTCCGCATGG CTCAGGAAGA GGCCCGTCGG AACAGGCTGA TGAGAGACAT GGCCCAGCTG 
CGACTTCAGC TCGAGGTCTC TCAGCTGGAA GGGAGCCTGC AGCAGCCCAA GGCCCAGTCA 
GCAATGTCTC CTTACCTCGT CCCGGACACC CAGGCCCTCT GCCACCACCT CCCGGTCATC 
CGCCAGCTGG CCACCAGCGG CCGCTTCATT GTCATCATCC CAAGGACAGT GATTGATGGC 
CTGGATTTGC TGAAGAAGGA ACACCCAGGG GCCCGGGATG GGATCCGTTA CCTGGAGGCA 
GAGTTTAAGA AAGGAAACAG GTACATTCGC TGCCAGAAAG AAGTAGGAAA GAGCTTCGAG 
CGGCATAAGC TGAAGAGGCA GGATGCAGAT GCCTGGACGC TCTATAAGAT CCTAGACAGC 
TGCAAACAGT TGACTTTGGC CCAGGGGGCA GGCGAGGAGG ACCCAAGCGG CATGGTGACC 
ATCGTCACAG GCTTTCCACT GGACAACCCC AGTGCGCTCT CAGGCCCCAT GCAGGCAGCC 
CTGCAGGCCG CTACACATGC CAGTGTGGAC ATCAAGAATG TTCTGGACTT CTACAAGCAG 
TGGAAGGAGA TTGGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013970793.1
CDS	173..3070
Translation	MSQGPPPGESSEPEAKVLHTKRLYRAVVEAVHRLDLILCNKTAYQEVFKPENISLRNKLRELCVKLMFLHPVDYGRKAEELLWRKVYYEVIQLIKTNKKHIHSRSTLECAYRTHLVAGIGFYQHLLLYIQSHYQLELQCCIDWTHVTDPLIGCKKPVSASGKEMDWAQMACHRCLMYLGDLSRYQNELAGVDTELLAERFYYQALSVAPQIGMPFNQLGTLAGSKYYNVEAMYCYLRCIQSEVSFEGAYGNLKRLYDKAAKMYHQLKKCETRKLSPSKKRCKDIKRLLVNFMYLQSLLQPRSSPVDSELTSLCQSVLEDFNLCLFYLPSSPSLSLASEGEEDYESGYAFLPDLLIFQMIIICLMGVHSLKRADEPLEKEEAPGPEPPPAVPQATEVRKSRKFSRLSCLRRRRHPPKAGDDSDLSEGFESDSSHDSARASEGSDSGSDKSLEGGGTAFDAETDSEMNSQESRSDLEDIEDEEGTRSPALEPPRPRSEAPESLNGPLGPSEASIASNLQAMSTQMFQTKRCFRLAPTFSNLLLQPSTEPHTVANRRSCVNGDADKPSEPASEEGSESEGSESSGHSCRNERSVQEKLQVLAAEGLLPAVKVFLDWLRANPDLIVVCAQSSQSLWNRLSVLLNLLPAAGELQESGLALCPEVQDLLEGCELPDLPSSLLLPEDTALRNLPPLRAAHRRFNFDTDRPLLNSLEESVVRICCIRSFGHFIAHLQGSILQFSAEAGIFVSIAQSEQEGVLQQAQAQFRMAQEEARRNRLMRDMAQLRLQLEVSQLEGSLQQPKAQSAMSPYLVPDTQALCHHLPVIRQLATSGRFIVIIPRTVIDGLDLLKKEHPGARDGIRYLEAEFKKGNRYIRCQKEVGKSFERHKLKRQDADAWTLYKILDSCKQLTLAQGAGEEDPSGMVTIVTGFPLDNPSALSGPMQAALQAATHASVDIKNVLDFYKQWKEIG

Target ORF information:

RefSeq Version	XM_014115318.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris SMG5 nonsense mediated mRNA decay factor (SMG5), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014115318.1

ORF Insert Sequence:

ATGAGCCAAG GCCCCCCCCC AGGGGAGAGC AGCGAGCCCG AGGCGAAGGT CCTCCATACC 
AAGCGGCTTT ACCGGGCTGT GGTGGAGGCT GTGCATCGAC TCGACCTCAT CCTTTGCAAC 
AAAACTGCTT ATCAAGAAGT GTTTAAACCA GAGAACATTA GCCTGAGGAA CAAGCTGCGT 
GAGCTCTGCG TCAAGCTTAT GTTCCTGCAC CCAGTAGACT ATGGGAGAAA GGCTGAGGAG 
CTGCTGTGGA GAAAGGTGTA CTACGAAGTT ATTCAGCTTA TCAAGACGAA TAAGAAGCAC 
ATCCACAGCC GGAGCACCTT GGAATGTGCC TACAGGACGC ACCTGGTTGC TGGGATTGGC 
TTCTACCAGC ATCTGCTGCT CTACATCCAG TCCCACTACC AGCTGGAGCT TCAGTGCTGC 
ATCGACTGGA CCCATGTTAC TGATCCCCTC ATAGGATGCA AGAAGCCAGT GTCTGCATCA 
GGGAAGGAGA TGGACTGGGC ACAGATGGCC TGCCACCGAT GTCTCATGTA CCTGGGGGAT 
TTGTCACGAT ATCAGAATGA ATTAGCTGGC GTAGACACTG AGCTGCTAGC TGAGAGATTT 
TACTACCAAG CCCTGTCTGT AGCTCCCCAG ATCGGAATGC CCTTTAACCA GCTGGGCACC 
CTTGCAGGCA GCAAGTACTA CAACGTGGAA GCCATGTACT GTTACCTGCG CTGCATCCAG 
TCGGAAGTGT CCTTTGAGGG GGCCTATGGG AATCTCAAGC GGCTGTATGA CAAGGCAGCC 
AAGATGTACC ACCAGCTGAA GAAGTGTGAG ACTCGGAAAC TCTCTCCCAG CAAGAAACGC 
TGTAAAGACA TCAAGAGGTT GCTGGTAAAC TTCATGTACC TTCAAAGCCT CCTGCAGCCC 
AGGAGCAGCC CGGTGGACTC AGAGCTGACC TCACTCTGCC AGTCAGTCCT GGAGGACTTC 
AATCTCTGCC TCTTCTACCT GCCATCCTCA CCCAGCCTCA GCCTGGCCAG TGAGGGTGAA 
GAGGACTATG AAAGTGGCTA TGCCTTCCTC CCAGACCTCC TCATCTTTCA GATGATCATC 
ATTTGTCTCA TGGGTGTGCA TAGCTTGAAG AGAGCAGATG AGCCCTTGGA GAAGGAGGAG 
GCACCGGGGC CTGAGCCCCC TCCTGCAGTA CCTCAAGCGA CTGAGGTCAG AAAGAGCCGG 
AAGTTCTCTC GCCTCTCCTG CCTCCGCCGC CGCCGCCACC CACCCAAAGC TGGAGATGAC 
AGTGACCTGA GTGAAGGCTT TGAATCAGAC TCGAGCCATG ACTCAGCCAG GGCCAGTGAG 
GGCTCAGACA GTGGCTCCGA CAAGAGTCTC GAAGGTGGGG GAACAGCCTT TGATGCTGAG 
ACAGACTCAG AAATGAACAG CCAAGAGTCC CGGTCAGACC TGGAAGACAT TGAGGATGAG 
GAGGGTACTC GGTCCCCAGC CCTGGAGCCC CCTCGGCCCA GGTCAGAGGC TCCCGAGTCT 
CTCAATGGCC CACTGGGCCC CAGTGAGGCG AGCATTGCCA GCAATCTACA GGCCATGTCC 
ACGCAGATGT TCCAGACCAA GCGCTGCTTC CGACTGGCGC CCACCTTCAG CAACCTGCTC 
CTCCAGCCTT CCACTGAGCC CCACACTGTA GCCAACCGCA GGTCATGTGT CAATGGAGAT 
GCAGACAAGC CTTCGGAACC AGCCTCCGAG GAGGGCTCCG AGTCGGAGGG CAGTGAATCT 
AGTGGGCACT CCTGTCGGAA TGAGCGCAGT GTCCAGGAGA AGCTGCAAGT CCTGGCAGCC 
GAAGGCCTGC TTCCTGCTGT GAAGGTCTTC CTGGACTGGC TGCGGGCCAA CCCTGACCTC 
ATCGTTGTGT GTGCACAGAG CTCTCAAAGT CTGTGGAATC GCCTGTCTGT GTTGCTGAAC 
CTGTTGCCAG CTGCTGGTGA GCTCCAAGAG TCTGGCCTGG CCCTGTGTCC CGAGGTCCAG 
GACCTACTTG AAGGTTGTGA ACTGCCTGAC CTGCCCTCCA GTCTGCTGCT CCCAGAGGAC 
ACGGCCCTTC GTAACCTGCC TCCCCTCCGG GCTGCCCATA GACGCTTCAA CTTTGACACA 
GACCGGCCCC TGCTTAACTC CTTAGAGGAG TCAGTCGTGC GCATCTGCTG CATCCGCAGC 
TTTGGACACT TCATCGCCCA CCTGCAGGGC AGCATCCTGC AGTTCAGCGC AGAGGCTGGC 
ATCTTTGTCA GCATCGCCCA GTCTGAGCAG GAGGGCGTGC TGCAGCAGGC CCAGGCGCAG 
TTCCGCATGG CTCAGGAAGA GGCCCGTCGG AACAGGCTGA TGAGAGACAT GGCCCAGCTG 
CGACTTCAGC TCGAGGTCTC TCAGCTGGAA GGGAGCCTGC AGCAGCCCAA GGCCCAGTCA 
GCAATGTCTC CTTACCTCGT CCCGGACACC CAGGCCCTCT GCCACCACCT CCCGGTCATC 
CGCCAGCTGG CCACCAGCGG CCGCTTCATT GTCATCATCC CAAGGACAGT GATTGATGGC 
CTGGATTTGC TGAAGAAGGA ACACCCAGGG GCCCGGGATG GGATCCGTTA CCTGGAGGCA 
GAGTTTAAGA AAGGAAACAG GTACATTCGC TGCCAGAAAG AAGTAGGAAA GAGCTTCGAG 
CGGCATAAGC TGAAGAGGCA GGATGCAGAT GCCTGGACGC TCTATAAGAT CCTAGACAGC 
TGCAAACAGT TGACTTTGGC CCAGGGGGCA GGCGAGGAGG ACCCAAGCGG CATGGTGACC 
ATCGTCACAG GCTTTCCACT GGACAACCCC AGTGCGCTCT CAGGCCCCAT GCAGGCAGCC 
CTGCAGGCCG CTACACATGC CAGTGTGGAC ATCAAGAATG TTCTGGACTT CTACAAGCAG 
TGGAAGGAGA TTGGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMG5 ( XM_547535.7 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_547535.3 Canis lupus familiaris SMG5, nonsense mediated mRNA decay factor (SMG5), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCe09508
Clone ID Related Accession (Same CDS sequence)	XM_547535.7 , XM_547535.6
Accession Version	XM_547535.7 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3036bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	protein SMG5 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006589.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_547535.6. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCCAAG GCCCCCCCCC AGGGGAGAGC AGCGAGCCCG AGGCGAAGGT CCTCCATACC 
AAGCGGCTTT ACCGGGCTGT GGTGGAGGCT GTGCATCGAC TCGACCTCAT CCTTTGCAAC 
AAAACTGCTT ATCAAGAAGT GTTTAAACCA GAGAACATTA GCCTGAGGAA CAAGCTGCGT 
GAGCTCTGCG TCAAGCTTAT GTTCCTGCAC CCAGTAGACT ATGGGAGAAA GGCTGAGGAG 
CTGCTGTGGA GAAAGGTGTA CTACGAAGTT ATTCAGCTTA TCAAGACGAA TAAGAAGCAC 
ATCCACAGCC GGAGCACCTT GGAATGTGCC TACAGGACGC ACCTGGTTGC TGGGATTGGC 
TTCTACCAGC ATCTGCTGCT CTACATCCAG TCCCACTACC AGCTGGAGCT TCAGTGCTGC 
ATCGACTGGA CCCATGTTAC TGATCCCCTC ATAGGATGCA AGAAGCCAGT GTCTGCATCA 
GGGAAGGAGA TGGACTGGGC ACAGATGGCC TGCCACCGAT GTCTCATGTA CCTGGGGGAT 
TTGTCACGAT ATCAGAATGA ATTAGCTGGC GTAGACACTG AGCTGCTAGC TGAGAGATTT 
TACTACCAAG CCCTGTCTGT AGCTCCCCAG ATCGGAATGC CCTTTAACCA GCTGGGCACC 
CTTGCAGGCA GCAAGTACTA CAACGTGGAA GCCATGTACT GTTACCTGCG CTGCATCCAG 
TCGGAAGTGT CCTTTGAGGG GGCCTATGGG AATCTCAAGC GGCTGTATGA CAAGGCAGCC 
AAGATGTACC ACCAGCTGAA GAAGTGTGAG ACTCGGAAAC TCTCTCCCAG CAAGAAACGC 
TGTAAAGACA TCAAGAGGTT GCTGGTAAAC TTCATGTACC TTCAAAGCCT CCTGCAGCCC 
AGGAGCAGCC CGGTGGACTC AGAGCTGACC TCACTCTGCC AGTCAGTCCT GGAGGACTTC 
AATCTCTGCC TCTTCTACCT GCCATCCTCA CCCAGCCTCA GCCTGGCCAG TGAGGGTGAA 
GAGGACTATG AAAGTGGCTA TGCCTTCCTC CCAGACCTCC TCATCTTTCA GATGATCATC 
ATTTGTCTCA TGGGTGTGCA TAGCTTGAAG AGAGCAGGGT CCAAACAGTA CAGTGCAGCC 
ATTGCCTTTA CCCTGGCCCT CTTCTCCCAC CTCGTCAACC ATGTCAATAT ACGGCTGCAG 
GCTGAGCTGG AGGAGGGTGA GAATCCCGTG CCGGCGTTCC AGAGCGATGG CACAGATGAG 
CCCTTGGAGA AGGAGGAGGC ACCGGGGCCT GAGCCCCCTC CTGCAGTACC TCAAGCGACT 
GAGGTCAGAA AGAGCCGGAA GTTCTCTCGC CTCTCCTGCC TCCGCCGCCG CCGCCACCCA 
CCCAAAGCTG GAGATGACAG TGACCTGAGT GAAGGCTTTG AATCAGACTC GAGCCATGAC 
TCAGCCAGGG CCAGTGAGGG CTCAGACAGT GGCTCCGACA AGAGTCTCGA AGGTGGGGGA 
ACAGCCTTTG ATGCTGAGAC AGACTCAGAA ATGAACAGCC AAGAGTCCCG GTCAGACCTG 
GAAGACATTG AGGATGAGGA GGGTACTCGG TCCCCAGCCC TGGAGCCCCC TCGGCCCAGG 
TCAGAGGCTC CCGAGTCTCT CAATGGCCCA CTGGGCCCCA GTGAGGCGAG CATTGCCAGC 
AATCTACAGG CCATGTCCAC GCAGATGTTC CAGACCAAGC GCTGCTTCCG ACTGGCGCCC 
ACCTTCAGCA ACCTGCTCCT CCAGCCTTCC ACTGAGCCCC ACACTGTAGC CAACCGCAGG 
TCATGTGTCA ATGGAGATGC AGACAAGCCT TCGGAACCAG CCTCCGAGGA GGGCTCCGAG 
TCGGAGGGCA GTGAATCTAG TGGGCACTCC TGTCGGAATG AGCGCAGTGT CCAGGAGAAG 
CTGCAAGTCC TGGCAGCCGA AGGCCTGCTT CCTGCTGTGA AGGTCTTCCT GGACTGGCTG 
CGGGCCAACC CTGACCTCAT CGTTGTGTGT GCACAGAGCT CTCAAAGTCT GTGGAATCGC 
CTGTCTGTGT TGCTGAACCT GTTGCCAGCT GCTGGTGAGC TCCAAGAGTC TGGCCTGGCC 
CTGTGTCCCG AGGTCCAGGA CCTACTTGAA GGTTGTGAAC TGCCTGACCT GCCCTCCAGT 
CTGCTGCTCC CAGAGGACAC GGCCCTTCGT AACCTGCCTC CCCTCCGGGC TGCCCATAGA 
CGCTTCAACT TTGACACAGA CCGGCCCCTG CTTAACTCCT TAGAGGAGTC AGTCGTGCGC 
ATCTGCTGCA TCCGCAGCTT TGGACACTTC ATCGCCCACC TGCAGGGCAG CATCCTGCAG 
TTCAGCGCAG AGGCTGGCAT CTTTGTCAGC ATCGCCCAGT CTGAGCAGGA GGGCGTGCTG 
CAGCAGGCCC AGGCGCAGTT CCGCATGGCT CAGGAAGAGG CCCGTCGGAA CAGGCTGATG 
AGAGACATGG CCCAGCTGCG ACTTCAGCTC GAGGTCTCTC AGCTGGAAGG GAGCCTGCAG 
CAGCCCAAGG CCCAGTCAGC AATGTCTCCT TACCTCGTCC CGGACACCCA GGCCCTCTGC 
CACCACCTCC CGGTCATCCG CCAGCTGGCC ACCAGCGGCC GCTTCATTGT CATCATCCCA 
AGGACAGTGA TTGATGGCCT GGATTTGCTG AAGAAGGAAC ACCCAGGGGC CCGGGATGGG 
ATCCGTTACC TGGAGGCAGA GTTTAAGAAA GGAAACAGGT ACATTCGCTG CCAGAAAGAA 
GTAGGAAAGA GCTTCGAGCG GCATAAGCTG AAGAGGCAGG ATGCAGATGC CTGGACGCTC 
TATAAGATCC TAGACAGCTG CAAACAGTTG ACTTTGGCCC AGGGGGCAGG CGAGGAGGAC 
CCAAGCGGCA TGGTGACCAT CGTCACAGGC TTTCCACTGG ACAACCCCAG TGCGCTCTCA 
GGCCCCATGC AGGCAGCCCT GCAGGCCGCT ACACATGCCA GTGTGGACAT CAAGAATGTT 
CTGGACTTCT ACAAGCAGTG GAAGGAGATT GGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_547535.3
CDS	146..3181
Translation	MSQGPPPGESSEPEAKVLHTKRLYRAVVEAVHRLDLILCNKTAYQEVFKPENISLRNKLRELCVKLMFLHPVDYGRKAEELLWRKVYYEVIQLIKTNKKHIHSRSTLECAYRTHLVAGIGFYQHLLLYIQSHYQLELQCCIDWTHVTDPLIGCKKPVSASGKEMDWAQMACHRCLMYLGDLSRYQNELAGVDTELLAERFYYQALSVAPQIGMPFNQLGTLAGSKYYNVEAMYCYLRCIQSEVSFEGAYGNLKRLYDKAAKMYHQLKKCETRKLSPSKKRCKDIKRLLVNFMYLQSLLQPRSSPVDSELTSLCQSVLEDFNLCLFYLPSSPSLSLASEGEEDYESGYAFLPDLLIFQMIIICLMGVHSLKRAGSKQYSAAIAFTLALFSHLVNHVNIRLQAELEEGENPVPAFQSDGTDEPLEKEEAPGPEPPPAVPQATEVRKSRKFSRLSCLRRRRHPPKAGDDSDLSEGFESDSSHDSARASEGSDSGSDKSLEGGGTAFDAETDSEMNSQESRSDLEDIEDEEGTRSPALEPPRPRSEAPESLNGPLGPSEASIASNLQAMSTQMFQTKRCFRLAPTFSNLLLQPSTEPHTVANRRSCVNGDADKPSEPASEEGSESEGSESSGHSCRNERSVQEKLQVLAAEGLLPAVKVFLDWLRANPDLIVVCAQSSQSLWNRLSVLLNLLPAAGELQESGLALCPEVQDLLEGCELPDLPSSLLLPEDTALRNLPPLRAAHRRFNFDTDRPLLNSLEESVVRICCIRSFGHFIAHLQGSILQFSAEAGIFVSIAQSEQEGVLQQAQAQFRMAQEEARRNRLMRDMAQLRLQLEVSQLEGSLQQPKAQSAMSPYLVPDTQALCHHLPVIRQLATSGRFIVIIPRTVIDGLDLLKKEHPGARDGIRYLEAEFKKGNRYIRCQKEVGKSFERHKLKRQDADAWTLYKILDSCKQLTLAQGAGEEDPSGMVTIVTGFPLDNPSALSGPMQAALQAATHASVDIKNVLDFYKQWKEIG

Target ORF information:

RefSeq Version	XM_547535.7
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris SMG5, nonsense mediated mRNA decay factor (SMG5), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_547535.7

ORF Insert Sequence:

ATGAGCCAAG GCCCCCCCCC AGGGGAGAGC AGCGAGCCCG AGGCGAAGGT CCTCCATACC 
AAGCGGCTTT ACCGGGCTGT GGTGGAGGCT GTGCATCGAC TCGACCTCAT CCTTTGCAAC 
AAAACTGCTT ATCAAGAAGT GTTTAAACCA GAGAACATTA GCCTGAGGAA CAAGCTGCGT 
GAGCTCTGCG TCAAGCTTAT GTTCCTGCAC CCAGTAGACT ATGGGAGAAA GGCTGAGGAG 
CTGCTGTGGA GAAAGGTGTA CTACGAAGTT ATTCAGCTTA TCAAGACGAA TAAGAAGCAC 
ATCCACAGCC GGAGCACCTT GGAATGTGCC TACAGGACGC ACCTGGTTGC TGGGATTGGC 
TTCTACCAGC ATCTGCTGCT CTACATCCAG TCCCACTACC AGCTGGAGCT TCAGTGCTGC 
ATCGACTGGA CCCATGTTAC TGATCCCCTC ATAGGATGCA AGAAGCCAGT GTCTGCATCA 
GGGAAGGAGA TGGACTGGGC ACAGATGGCC TGCCACCGAT GTCTCATGTA CCTGGGGGAT 
TTGTCACGAT ATCAGAATGA ATTAGCTGGC GTAGACACTG AGCTGCTAGC TGAGAGATTT 
TACTACCAAG CCCTGTCTGT AGCTCCCCAG ATCGGAATGC CCTTTAACCA GCTGGGCACC 
CTTGCAGGCA GCAAGTACTA CAACGTGGAA GCCATGTACT GTTACCTGCG CTGCATCCAG 
TCGGAAGTGT CCTTTGAGGG GGCCTATGGG AATCTCAAGC GGCTGTATGA CAAGGCAGCC 
AAGATGTACC ACCAGCTGAA GAAGTGTGAG ACTCGGAAAC TCTCTCCCAG CAAGAAACGC 
TGTAAAGACA TCAAGAGGTT GCTGGTAAAC TTCATGTACC TTCAAAGCCT CCTGCAGCCC 
AGGAGCAGCC CGGTGGACTC AGAGCTGACC TCACTCTGCC AGTCAGTCCT GGAGGACTTC 
AATCTCTGCC TCTTCTACCT GCCATCCTCA CCCAGCCTCA GCCTGGCCAG TGAGGGTGAA 
GAGGACTATG AAAGTGGCTA TGCCTTCCTC CCAGACCTCC TCATCTTTCA GATGATCATC 
ATTTGTCTCA TGGGTGTGCA TAGCTTGAAG AGAGCAGGGT CCAAACAGTA CAGTGCAGCC 
ATTGCCTTTA CCCTGGCCCT CTTCTCCCAC CTCGTCAACC ATGTCAATAT ACGGCTGCAG 
GCTGAGCTGG AGGAGGGTGA GAATCCCGTG CCGGCGTTCC AGAGCGATGG CACAGATGAG 
CCCTTGGAGA AGGAGGAGGC ACCGGGGCCT GAGCCCCCTC CTGCAGTACC TCAAGCGACT 
GAGGTCAGAA AGAGCCGGAA GTTCTCTCGC CTCTCCTGCC TCCGCCGCCG CCGCCACCCA 
CCCAAAGCTG GAGATGACAG TGACCTGAGT GAAGGCTTTG AATCAGACTC GAGCCATGAC 
TCAGCCAGGG CCAGTGAGGG CTCAGACAGT GGCTCCGACA AGAGTCTCGA AGGTGGGGGA 
ACAGCCTTTG ATGCTGAGAC AGACTCAGAA ATGAACAGCC AAGAGTCCCG GTCAGACCTG 
GAAGACATTG AGGATGAGGA GGGTACTCGG TCCCCAGCCC TGGAGCCCCC TCGGCCCAGG 
TCAGAGGCTC CCGAGTCTCT CAATGGCCCA CTGGGCCCCA GTGAGGCGAG CATTGCCAGC 
AATCTACAGG CCATGTCCAC GCAGATGTTC CAGACCAAGC GCTGCTTCCG ACTGGCGCCC 
ACCTTCAGCA ACCTGCTCCT CCAGCCTTCC ACTGAGCCCC ACACTGTAGC CAACCGCAGG 
TCATGTGTCA ATGGAGATGC AGACAAGCCT TCGGAACCAG CCTCCGAGGA GGGCTCCGAG 
TCGGAGGGCA GTGAATCTAG TGGGCACTCC TGTCGGAATG AGCGCAGTGT CCAGGAGAAG 
CTGCAAGTCC TGGCAGCCGA AGGCCTGCTT CCTGCTGTGA AGGTCTTCCT GGACTGGCTG 
CGGGCCAACC CTGACCTCAT CGTTGTGTGT GCACAGAGCT CTCAAAGTCT GTGGAATCGC 
CTGTCTGTGT TGCTGAACCT GTTGCCAGCT GCTGGTGAGC TCCAAGAGTC TGGCCTGGCC 
CTGTGTCCCG AGGTCCAGGA CCTACTTGAA GGTTGTGAAC TGCCTGACCT GCCCTCCAGT 
CTGCTGCTCC CAGAGGACAC GGCCCTTCGT AACCTGCCTC CCCTCCGGGC TGCCCATAGA 
CGCTTCAACT TTGACACAGA CCGGCCCCTG CTTAACTCCT TAGAGGAGTC AGTCGTGCGC 
ATCTGCTGCA TCCGCAGCTT TGGACACTTC ATCGCCCACC TGCAGGGCAG CATCCTGCAG 
TTCAGCGCAG AGGCTGGCAT CTTTGTCAGC ATCGCCCAGT CTGAGCAGGA GGGCGTGCTG 
CAGCAGGCCC AGGCGCAGTT CCGCATGGCT CAGGAAGAGG CCCGTCGGAA CAGGCTGATG 
AGAGACATGG CCCAGCTGCG ACTTCAGCTC GAGGTCTCTC AGCTGGAAGG GAGCCTGCAG 
CAGCCCAAGG CCCAGTCAGC AATGTCTCCT TACCTCGTCC CGGACACCCA GGCCCTCTGC 
CACCACCTCC CGGTCATCCG CCAGCTGGCC ACCAGCGGCC GCTTCATTGT CATCATCCCA 
AGGACAGTGA TTGATGGCCT GGATTTGCTG AAGAAGGAAC ACCCAGGGGC CCGGGATGGG 
ATCCGTTACC TGGAGGCAGA GTTTAAGAAA GGAAACAGGT ACATTCGCTG CCAGAAAGAA 
GTAGGAAAGA GCTTCGAGCG GCATAAGCTG AAGAGGCAGG ATGCAGATGC CTGGACGCTC 
TATAAGATCC TAGACAGCTG CAAACAGTTG ACTTTGGCCC AGGGGGCAGG CGAGGAGGAC 
CCAAGCGGCA TGGTGACCAT CGTCACAGGC TTTCCACTGG ACAACCCCAG TGCGCTCTCA 
GGCCCCATGC AGGCAGCCCT GCAGGCCGCT ACACATGCCA GTGTGGACAT CAAGAATGTT 
CTGGACTTCT ACAAGCAGTG GAAGGAGATT GGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMG5 ( XM_547535.6 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_547535.3 Canis lupus familiaris SMG5 nonsense mediated mRNA decay factor (SMG5), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe09508
Clone ID Related Accession (Same CDS sequence)	XM_547535.7 , XM_547535.6
Accession Version	XM_547535.6	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3036bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	protein SMG5 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726067.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_547535.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCCAAG GCCCCCCCCC AGGGGAGAGC AGCGAGCCCG AGGCGAAGGT CCTCCATACC 
AAGCGGCTTT ACCGGGCTGT GGTGGAGGCT GTGCATCGAC TCGACCTCAT CCTTTGCAAC 
AAAACTGCTT ATCAAGAAGT GTTTAAACCA GAGAACATTA GCCTGAGGAA CAAGCTGCGT 
GAGCTCTGCG TCAAGCTTAT GTTCCTGCAC CCAGTAGACT ATGGGAGAAA GGCTGAGGAG 
CTGCTGTGGA GAAAGGTGTA CTACGAAGTT ATTCAGCTTA TCAAGACGAA TAAGAAGCAC 
ATCCACAGCC GGAGCACCTT GGAATGTGCC TACAGGACGC ACCTGGTTGC TGGGATTGGC 
TTCTACCAGC ATCTGCTGCT CTACATCCAG TCCCACTACC AGCTGGAGCT TCAGTGCTGC 
ATCGACTGGA CCCATGTTAC TGATCCCCTC ATAGGATGCA AGAAGCCAGT GTCTGCATCA 
GGGAAGGAGA TGGACTGGGC ACAGATGGCC TGCCACCGAT GTCTCATGTA CCTGGGGGAT 
TTGTCACGAT ATCAGAATGA ATTAGCTGGC GTAGACACTG AGCTGCTAGC TGAGAGATTT 
TACTACCAAG CCCTGTCTGT AGCTCCCCAG ATCGGAATGC CCTTTAACCA GCTGGGCACC 
CTTGCAGGCA GCAAGTACTA CAACGTGGAA GCCATGTACT GTTACCTGCG CTGCATCCAG 
TCGGAAGTGT CCTTTGAGGG GGCCTATGGG AATCTCAAGC GGCTGTATGA CAAGGCAGCC 
AAGATGTACC ACCAGCTGAA GAAGTGTGAG ACTCGGAAAC TCTCTCCCAG CAAGAAACGC 
TGTAAAGACA TCAAGAGGTT GCTGGTAAAC TTCATGTACC TTCAAAGCCT CCTGCAGCCC 
AGGAGCAGCC CGGTGGACTC AGAGCTGACC TCACTCTGCC AGTCAGTCCT GGAGGACTTC 
AATCTCTGCC TCTTCTACCT GCCATCCTCA CCCAGCCTCA GCCTGGCCAG TGAGGGTGAA 
GAGGACTATG AAAGTGGCTA TGCCTTCCTC CCAGACCTCC TCATCTTTCA GATGATCATC 
ATTTGTCTCA TGGGTGTGCA TAGCTTGAAG AGAGCAGGGT CCAAACAGTA CAGTGCAGCC 
ATTGCCTTTA CCCTGGCCCT CTTCTCCCAC CTCGTCAACC ATGTCAATAT ACGGCTGCAG 
GCTGAGCTGG AGGAGGGTGA GAATCCCGTG CCGGCGTTCC AGAGCGATGG CACAGATGAG 
CCCTTGGAGA AGGAGGAGGC ACCGGGGCCT GAGCCCCCTC CTGCAGTACC TCAAGCGACT 
GAGGTCAGAA AGAGCCGGAA GTTCTCTCGC CTCTCCTGCC TCCGCCGCCG CCGCCACCCA 
CCCAAAGCTG GAGATGACAG TGACCTGAGT GAAGGCTTTG AATCAGACTC GAGCCATGAC 
TCAGCCAGGG CCAGTGAGGG CTCAGACAGT GGCTCCGACA AGAGTCTCGA AGGTGGGGGA 
ACAGCCTTTG ATGCTGAGAC AGACTCAGAA ATGAACAGCC AAGAGTCCCG GTCAGACCTG 
GAAGACATTG AGGATGAGGA GGGTACTCGG TCCCCAGCCC TGGAGCCCCC TCGGCCCAGG 
TCAGAGGCTC CCGAGTCTCT CAATGGCCCA CTGGGCCCCA GTGAGGCGAG CATTGCCAGC 
AATCTACAGG CCATGTCCAC GCAGATGTTC CAGACCAAGC GCTGCTTCCG ACTGGCGCCC 
ACCTTCAGCA ACCTGCTCCT CCAGCCTTCC ACTGAGCCCC ACACTGTAGC CAACCGCAGG 
TCATGTGTCA ATGGAGATGC AGACAAGCCT TCGGAACCAG CCTCCGAGGA GGGCTCCGAG 
TCGGAGGGCA GTGAATCTAG TGGGCACTCC TGTCGGAATG AGCGCAGTGT CCAGGAGAAG 
CTGCAAGTCC TGGCAGCCGA AGGCCTGCTT CCTGCTGTGA AGGTCTTCCT GGACTGGCTG 
CGGGCCAACC CTGACCTCAT CGTTGTGTGT GCACAGAGCT CTCAAAGTCT GTGGAATCGC 
CTGTCTGTGT TGCTGAACCT GTTGCCAGCT GCTGGTGAGC TCCAAGAGTC TGGCCTGGCC 
CTGTGTCCCG AGGTCCAGGA CCTACTTGAA GGTTGTGAAC TGCCTGACCT GCCCTCCAGT 
CTGCTGCTCC CAGAGGACAC GGCCCTTCGT AACCTGCCTC CCCTCCGGGC TGCCCATAGA 
CGCTTCAACT TTGACACAGA CCGGCCCCTG CTTAACTCCT TAGAGGAGTC AGTCGTGCGC 
ATCTGCTGCA TCCGCAGCTT TGGACACTTC ATCGCCCACC TGCAGGGCAG CATCCTGCAG 
TTCAGCGCAG AGGCTGGCAT CTTTGTCAGC ATCGCCCAGT CTGAGCAGGA GGGCGTGCTG 
CAGCAGGCCC AGGCGCAGTT CCGCATGGCT CAGGAAGAGG CCCGTCGGAA CAGGCTGATG 
AGAGACATGG CCCAGCTGCG ACTTCAGCTC GAGGTCTCTC AGCTGGAAGG GAGCCTGCAG 
CAGCCCAAGG CCCAGTCAGC AATGTCTCCT TACCTCGTCC CGGACACCCA GGCCCTCTGC 
CACCACCTCC CGGTCATCCG CCAGCTGGCC ACCAGCGGCC GCTTCATTGT CATCATCCCA 
AGGACAGTGA TTGATGGCCT GGATTTGCTG AAGAAGGAAC ACCCAGGGGC CCGGGATGGG 
ATCCGTTACC TGGAGGCAGA GTTTAAGAAA GGAAACAGGT ACATTCGCTG CCAGAAAGAA 
GTAGGAAAGA GCTTCGAGCG GCATAAGCTG AAGAGGCAGG ATGCAGATGC CTGGACGCTC 
TATAAGATCC TAGACAGCTG CAAACAGTTG ACTTTGGCCC AGGGGGCAGG CGAGGAGGAC 
CCAAGCGGCA TGGTGACCAT CGTCACAGGC TTTCCACTGG ACAACCCCAG TGCGCTCTCA 
GGCCCCATGC AGGCAGCCCT GCAGGCCGCT ACACATGCCA GTGTGGACAT CAAGAATGTT 
CTGGACTTCT ACAAGCAGTG GAAGGAGATT GGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_547535.3
CDS	173..3208
Translation	MSQGPPPGESSEPEAKVLHTKRLYRAVVEAVHRLDLILCNKTAYQEVFKPENISLRNKLRELCVKLMFLHPVDYGRKAEELLWRKVYYEVIQLIKTNKKHIHSRSTLECAYRTHLVAGIGFYQHLLLYIQSHYQLELQCCIDWTHVTDPLIGCKKPVSASGKEMDWAQMACHRCLMYLGDLSRYQNELAGVDTELLAERFYYQALSVAPQIGMPFNQLGTLAGSKYYNVEAMYCYLRCIQSEVSFEGAYGNLKRLYDKAAKMYHQLKKCETRKLSPSKKRCKDIKRLLVNFMYLQSLLQPRSSPVDSELTSLCQSVLEDFNLCLFYLPSSPSLSLASEGEEDYESGYAFLPDLLIFQMIIICLMGVHSLKRAGSKQYSAAIAFTLALFSHLVNHVNIRLQAELEEGENPVPAFQSDGTDEPLEKEEAPGPEPPPAVPQATEVRKSRKFSRLSCLRRRRHPPKAGDDSDLSEGFESDSSHDSARASEGSDSGSDKSLEGGGTAFDAETDSEMNSQESRSDLEDIEDEEGTRSPALEPPRPRSEAPESLNGPLGPSEASIASNLQAMSTQMFQTKRCFRLAPTFSNLLLQPSTEPHTVANRRSCVNGDADKPSEPASEEGSESEGSESSGHSCRNERSVQEKLQVLAAEGLLPAVKVFLDWLRANPDLIVVCAQSSQSLWNRLSVLLNLLPAAGELQESGLALCPEVQDLLEGCELPDLPSSLLLPEDTALRNLPPLRAAHRRFNFDTDRPLLNSLEESVVRICCIRSFGHFIAHLQGSILQFSAEAGIFVSIAQSEQEGVLQQAQAQFRMAQEEARRNRLMRDMAQLRLQLEVSQLEGSLQQPKAQSAMSPYLVPDTQALCHHLPVIRQLATSGRFIVIIPRTVIDGLDLLKKEHPGARDGIRYLEAEFKKGNRYIRCQKEVGKSFERHKLKRQDADAWTLYKILDSCKQLTLAQGAGEEDPSGMVTIVTGFPLDNPSALSGPMQAALQAATHASVDIKNVLDFYKQWKEIG

Target ORF information:

RefSeq Version	XM_547535.6
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris SMG5 nonsense mediated mRNA decay factor (SMG5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_547535.6

ORF Insert Sequence:

ATGAGCCAAG GCCCCCCCCC AGGGGAGAGC AGCGAGCCCG AGGCGAAGGT CCTCCATACC 
AAGCGGCTTT ACCGGGCTGT GGTGGAGGCT GTGCATCGAC TCGACCTCAT CCTTTGCAAC 
AAAACTGCTT ATCAAGAAGT GTTTAAACCA GAGAACATTA GCCTGAGGAA CAAGCTGCGT 
GAGCTCTGCG TCAAGCTTAT GTTCCTGCAC CCAGTAGACT ATGGGAGAAA GGCTGAGGAG 
CTGCTGTGGA GAAAGGTGTA CTACGAAGTT ATTCAGCTTA TCAAGACGAA TAAGAAGCAC 
ATCCACAGCC GGAGCACCTT GGAATGTGCC TACAGGACGC ACCTGGTTGC TGGGATTGGC 
TTCTACCAGC ATCTGCTGCT CTACATCCAG TCCCACTACC AGCTGGAGCT TCAGTGCTGC 
ATCGACTGGA CCCATGTTAC TGATCCCCTC ATAGGATGCA AGAAGCCAGT GTCTGCATCA 
GGGAAGGAGA TGGACTGGGC ACAGATGGCC TGCCACCGAT GTCTCATGTA CCTGGGGGAT 
TTGTCACGAT ATCAGAATGA ATTAGCTGGC GTAGACACTG AGCTGCTAGC TGAGAGATTT 
TACTACCAAG CCCTGTCTGT AGCTCCCCAG ATCGGAATGC CCTTTAACCA GCTGGGCACC 
CTTGCAGGCA GCAAGTACTA CAACGTGGAA GCCATGTACT GTTACCTGCG CTGCATCCAG 
TCGGAAGTGT CCTTTGAGGG GGCCTATGGG AATCTCAAGC GGCTGTATGA CAAGGCAGCC 
AAGATGTACC ACCAGCTGAA GAAGTGTGAG ACTCGGAAAC TCTCTCCCAG CAAGAAACGC 
TGTAAAGACA TCAAGAGGTT GCTGGTAAAC TTCATGTACC TTCAAAGCCT CCTGCAGCCC 
AGGAGCAGCC CGGTGGACTC AGAGCTGACC TCACTCTGCC AGTCAGTCCT GGAGGACTTC 
AATCTCTGCC TCTTCTACCT GCCATCCTCA CCCAGCCTCA GCCTGGCCAG TGAGGGTGAA 
GAGGACTATG AAAGTGGCTA TGCCTTCCTC CCAGACCTCC TCATCTTTCA GATGATCATC 
ATTTGTCTCA TGGGTGTGCA TAGCTTGAAG AGAGCAGGGT CCAAACAGTA CAGTGCAGCC 
ATTGCCTTTA CCCTGGCCCT CTTCTCCCAC CTCGTCAACC ATGTCAATAT ACGGCTGCAG 
GCTGAGCTGG AGGAGGGTGA GAATCCCGTG CCGGCGTTCC AGAGCGATGG CACAGATGAG 
CCCTTGGAGA AGGAGGAGGC ACCGGGGCCT GAGCCCCCTC CTGCAGTACC TCAAGCGACT 
GAGGTCAGAA AGAGCCGGAA GTTCTCTCGC CTCTCCTGCC TCCGCCGCCG CCGCCACCCA 
CCCAAAGCTG GAGATGACAG TGACCTGAGT GAAGGCTTTG AATCAGACTC GAGCCATGAC 
TCAGCCAGGG CCAGTGAGGG CTCAGACAGT GGCTCCGACA AGAGTCTCGA AGGTGGGGGA 
ACAGCCTTTG ATGCTGAGAC AGACTCAGAA ATGAACAGCC AAGAGTCCCG GTCAGACCTG 
GAAGACATTG AGGATGAGGA GGGTACTCGG TCCCCAGCCC TGGAGCCCCC TCGGCCCAGG 
TCAGAGGCTC CCGAGTCTCT CAATGGCCCA CTGGGCCCCA GTGAGGCGAG CATTGCCAGC 
AATCTACAGG CCATGTCCAC GCAGATGTTC CAGACCAAGC GCTGCTTCCG ACTGGCGCCC 
ACCTTCAGCA ACCTGCTCCT CCAGCCTTCC ACTGAGCCCC ACACTGTAGC CAACCGCAGG 
TCATGTGTCA ATGGAGATGC AGACAAGCCT TCGGAACCAG CCTCCGAGGA GGGCTCCGAG 
TCGGAGGGCA GTGAATCTAG TGGGCACTCC TGTCGGAATG AGCGCAGTGT CCAGGAGAAG 
CTGCAAGTCC TGGCAGCCGA AGGCCTGCTT CCTGCTGTGA AGGTCTTCCT GGACTGGCTG 
CGGGCCAACC CTGACCTCAT CGTTGTGTGT GCACAGAGCT CTCAAAGTCT GTGGAATCGC 
CTGTCTGTGT TGCTGAACCT GTTGCCAGCT GCTGGTGAGC TCCAAGAGTC TGGCCTGGCC 
CTGTGTCCCG AGGTCCAGGA CCTACTTGAA GGTTGTGAAC TGCCTGACCT GCCCTCCAGT 
CTGCTGCTCC CAGAGGACAC GGCCCTTCGT AACCTGCCTC CCCTCCGGGC TGCCCATAGA 
CGCTTCAACT TTGACACAGA CCGGCCCCTG CTTAACTCCT TAGAGGAGTC AGTCGTGCGC 
ATCTGCTGCA TCCGCAGCTT TGGACACTTC ATCGCCCACC TGCAGGGCAG CATCCTGCAG 
TTCAGCGCAG AGGCTGGCAT CTTTGTCAGC ATCGCCCAGT CTGAGCAGGA GGGCGTGCTG 
CAGCAGGCCC AGGCGCAGTT CCGCATGGCT CAGGAAGAGG CCCGTCGGAA CAGGCTGATG 
AGAGACATGG CCCAGCTGCG ACTTCAGCTC GAGGTCTCTC AGCTGGAAGG GAGCCTGCAG 
CAGCCCAAGG CCCAGTCAGC AATGTCTCCT TACCTCGTCC CGGACACCCA GGCCCTCTGC 
CACCACCTCC CGGTCATCCG CCAGCTGGCC ACCAGCGGCC GCTTCATTGT CATCATCCCA 
AGGACAGTGA TTGATGGCCT GGATTTGCTG AAGAAGGAAC ACCCAGGGGC CCGGGATGGG 
ATCCGTTACC TGGAGGCAGA GTTTAAGAAA GGAAACAGGT ACATTCGCTG CCAGAAAGAA 
GTAGGAAAGA GCTTCGAGCG GCATAAGCTG AAGAGGCAGG ATGCAGATGC CTGGACGCTC 
TATAAGATCC TAGACAGCTG CAAACAGTTG ACTTTGGCCC AGGGGGCAGG CGAGGAGGAC 
CCAAGCGGCA TGGTGACCAT CGTCACAGGC TTTCCACTGG ACAACCCCAG TGCGCTCTCA 
GGCCCCATGC AGGCAGCCCT GCAGGCCGCT ACACATGCCA GTGTGGACAT CAAGAATGTT 
CTGGACTTCT ACAAGCAGTG GAAGGAGATT GGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMG5 ( XM_022421261.1 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_022276969.1 Canis lupus familiaris SMG5, nonsense mediated mRNA decay factor (SMG5), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe289688
Clone ID Related Accession (Same CDS sequence)	XM_022421261.1
Accession Version	XM_022421261.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3141bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	protein SMG5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006589.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCCAAG GCCCCCCCCC AGGGGAGAGC AGCGAGCCCG AGGCGAAGGT CCTCCATACC 
AAGCGGCTTT ACCGGGCTGT GGTGGAGGCT GTGCATCGAC TCGACCTCAT CCTTTGCAAC 
AAAACTGCTT ATCAAGAAGT GTTTAAACCA GAGAACATTA GCCTGAGGAA CAAGCTGCGT 
GAGCTCTGCG TCAAGCTTAT GTTCCTGCAC CCAGTAGACT ATGGGAGAAA GGCTGAGGAG 
CTGCTGTGGA GAAAGGTGTA CTACGAAGTT ATTCAGCTTA TCAAGACGAA TAAGAAGCAC 
ATCCACAGCC GGAGCACCTT GGAATGTGCC TACAGGACGC ACCTGGTTGC TGGGATTGGC 
TTCTACCAGC ATCTGCTGCT CTACATCCAG TCCCACTACC AGCTGGAGCT TCAGTGCTGC 
ATCGACTGGA CCCATGTTAC TGATCCCCTC ATAGGATGCA AGAAGCCAGT GTCTGCATCA 
GGGAAGGAGA TGGACTGGGC ACAGATGGCC TGCCACCGAT GTCTCATGTA CCTGGGGGAT 
TTGTCACGAT ATCAGAATGA ATTAGCTGGC GTAGACACTG AGCTGCTAGC TGAGAGATTT 
TACTACCAAG CCCTGTCTGT AGCTCCCCAG ATCGGAATGC CCTTTAACCA GCTGGGCACC 
CTTGCAGGCA GCAAGTACTA CAACGTGGAA GCCATGTACT GTTACCTGCG CTGCATCCAG 
TCGGAAGTGT CCTTTGAGGG GGCCTATGGG AATCTCAAGC GGCTGTATGA CAAGGCAGCC 
AAGATGTACC ACCAGCTGAA GAAGTGTGAG ACTCGGAAAC TCTCTCCCAG CAAGAAACGC 
TGTAAAGACA TCAAGAGGTT GCTGGTAAAC TTCATGTACC TTCAAAGCCT CCTGCAGCCC 
AGGAGCAGCC CGGTGGACTC AGAGCTGACC TCACTCTGCC AGTCAGTCCT GGAGGACTTC 
AATCTCTGCC TCTTCTACCT GCCATCCTCA CCCAGCCTCA GCCTGGCCAG TGAGGGTGAA 
GAGGACTATG AAAGTGGCTA TGCCTTCCTC CCAGACCTCC TCATCTTTCA GATGATCATC 
ATTTGTCTCA TGGGTGTGCA TAGCTTGAAG AGAGCAGGGT CCAAACAGTA CAGTGCAGCC 
ATTGCCTTTA CCCTGGCCCT CTTCTCCCAC CTCGTCAACC ATGTCAATAT ACGGCTGCAG 
GCTGAGCTGG AGGAGGGTGA GAATCCCGTG CCGGCGTTCC AGAGCGATGG CACAGATGAG 
CCCTTGGAGA AGGAGGAGGC ACCGGGGCCT GAGCCCCCTC CTGCAGTACC TCAAGCGACT 
GAGGTCAGAA AGAGCCGGAA GTTCTCTCGC CTCTCCTGCC TCCGCCGCCG CCGCCACCCA 
CCCAAAGCTG GAGATGACAG TGACCTGAGT GAAGGCTTTG AATCAGACTC GAGCCATGAC 
TCAGCCAGGG CCAGTGAGGG CTCAGACAGT GGCTCCGACA AGAGTCTCGA AGGTGGGGGA 
ACAGCCTTTG ATGCTGAGAC AGACTCAGAA ATGAACAGCC AAGAGTCCCG GTCAGACCTG 
GAAGACATTG AGGATGAGGA GGGTACTCGG TCCCCAGCCC TGGAGCCCCC TCGGCCCAGG 
TCAGAGGCTC CCGAGTCTCT CAATGGCCCA CTGGGCCCCA GTGAGGCGAG CATTGCCAGC 
AATCTACAGG CCATGTCCAC GCAGATGTTC CAGACCAAGC GCTGCTTCCG ACTGGCGCCC 
ACCTTCAGCA ACCTGCTCCT CCAGCCTTCC ACTGAGCCCC ACACTGTAGC CAACCGCAGG 
TCATGTGTCA ATGGAGATGC AGACAAGCCT TCGGAACCAG AACTCTGCTC CAGAGTGCAG 
GCAGGGCGAT TGTGCCCTCC AGCTTGGGGC CGAAGCCCAA TGCCACGTGC CTGCTGTTCA 
TCCCAGCTCT GTCTCCCACT CCCAGCCTCC GAGGAGGGCT CCGAGTCGGA GGGCAGTGAA 
TCTAGTGGGC ACTCCTGTCG GAATGAGCGC AGTGTCCAGG AGAAGCTGCA AGTCCTGGCA 
GCCGAAGGCC TGCTTCCTGC TGTGAAGGTC TTCCTGGACT GGCTGCGGGC CAACCCTGAC 
CTCATCGTTG TGTGTGCACA GAGCTCTCAA AGTCTGTGGA ATCGCCTGTC TGTGTTGCTG 
AACCTGTTGC CAGCTGCTGG TGAGCTCCAA GAGTCTGGCC TGGCCCTGTG TCCCGAGGTC 
CAGGACCTAC TTGAAGGTTG TGAACTGCCT GACCTGCCCT CCAGTCTGCT GCTCCCAGAG 
GACACGGCCC TTCGTAACCT GCCTCCCCTC CGGGCTGCCC ATAGACGCTT CAACTTTGAC 
ACAGACCGGC CCCTGCTTAA CTCCTTAGAG GAGTCAGTCG TGCGCATCTG CTGCATCCGC 
AGCTTTGGAC ACTTCATCGC CCACCTGCAG GGCAGCATCC TGCAGTTCAG CGCAGAGGCT 
GGCATCTTTG TCAGCATCGC CCAGTCTGAG CAGGAGGGCG TGCTGCAGCA GGCCCAGGCG 
CAGTTCCGCA TGGCTCAGGA AGAGGCCCGT CGGAACAGGC TGATGAGAGA CATGGCCCAG 
CTGCGACTTC AGCTCGAGGT CTCTCAGCTG GAAGGGAGCC TGCAGCAGCC CAAGGCCCAG 
TCAGCAATGT CTCCTTACCT CGTCCCGGAC ACCCAGGCCC TCTGCCACCA CCTCCCGGTC 
ATCCGCCAGC TGGCCACCAG CGGCCGCTTC ATTGTCATCA TCCCAAGGAC AGTGATTGAT 
GGCCTGGATT TGCTGAAGAA GGAACACCCA GGGGCCCGGG ATGGGATCCG TTACCTGGAG 
GCAGAGTTTA AGAAAGGAAA CAGGTACATT CGCTGCCAGA AAGAAGTAGG AAAGAGCTTC 
GAGCGGCATA AGCTGAAGAG GCAGGATGCA GATGCCTGGA CGCTCTATAA GATCCTAGAC 
AGCTGCAAAC AGTTGACTTT GGCCCAGGGG GCAGGCGAGG AGGACCCAAG CGGCATGGTG 
ACCATCGTCA CAGGCTTTCC ACTGGACAAC CCCAGTGCGC TCTCAGGCCC CATGCAGGCA 
GCCCTGCAGG CCGCTACACA TGCCAGTGTG GACATCAAGA ATGTTCTGGA CTTCTACAAG 
CAGTGGAAGG AGATTGGTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022276969.1
CDS	146..3286
Translation	MSQGPPPGESSEPEAKVLHTKRLYRAVVEAVHRLDLILCNKTAYQEVFKPENISLRNKLRELCVKLMFLHPVDYGRKAEELLWRKVYYEVIQLIKTNKKHIHSRSTLECAYRTHLVAGIGFYQHLLLYIQSHYQLELQCCIDWTHVTDPLIGCKKPVSASGKEMDWAQMACHRCLMYLGDLSRYQNELAGVDTELLAERFYYQALSVAPQIGMPFNQLGTLAGSKYYNVEAMYCYLRCIQSEVSFEGAYGNLKRLYDKAAKMYHQLKKCETRKLSPSKKRCKDIKRLLVNFMYLQSLLQPRSSPVDSELTSLCQSVLEDFNLCLFYLPSSPSLSLASEGEEDYESGYAFLPDLLIFQMIIICLMGVHSLKRAGSKQYSAAIAFTLALFSHLVNHVNIRLQAELEEGENPVPAFQSDGTDEPLEKEEAPGPEPPPAVPQATEVRKSRKFSRLSCLRRRRHPPKAGDDSDLSEGFESDSSHDSARASEGSDSGSDKSLEGGGTAFDAETDSEMNSQESRSDLEDIEDEEGTRSPALEPPRPRSEAPESLNGPLGPSEASIASNLQAMSTQMFQTKRCFRLAPTFSNLLLQPSTEPHTVANRRSCVNGDADKPSEPELCSRVQAGRLCPPAWGRSPMPRACCSSQLCLPLPASEEGSESEGSESSGHSCRNERSVQEKLQVLAAEGLLPAVKVFLDWLRANPDLIVVCAQSSQSLWNRLSVLLNLLPAAGELQESGLALCPEVQDLLEGCELPDLPSSLLLPEDTALRNLPPLRAAHRRFNFDTDRPLLNSLEESVVRICCIRSFGHFIAHLQGSILQFSAEAGIFVSIAQSEQEGVLQQAQAQFRMAQEEARRNRLMRDMAQLRLQLEVSQLEGSLQQPKAQSAMSPYLVPDTQALCHHLPVIRQLATSGRFIVIIPRTVIDGLDLLKKEHPGARDGIRYLEAEFKKGNRYIRCQKEVGKSFERHKLKRQDADAWTLYKILDSCKQLTLAQGAGEEDPSGMVTIVTGFPLDNPSALSGPMQAALQAATHASVDIKNVLDFYKQWKEIG

Target ORF information:

RefSeq Version	XM_022421261.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris SMG5, nonsense mediated mRNA decay factor (SMG5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022421261.1

ORF Insert Sequence:

ATGAGCCAAG GCCCCCCCCC AGGGGAGAGC AGCGAGCCCG AGGCGAAGGT CCTCCATACC 
AAGCGGCTTT ACCGGGCTGT GGTGGAGGCT GTGCATCGAC TCGACCTCAT CCTTTGCAAC 
AAAACTGCTT ATCAAGAAGT GTTTAAACCA GAGAACATTA GCCTGAGGAA CAAGCTGCGT 
GAGCTCTGCG TCAAGCTTAT GTTCCTGCAC CCAGTAGACT ATGGGAGAAA GGCTGAGGAG 
CTGCTGTGGA GAAAGGTGTA CTACGAAGTT ATTCAGCTTA TCAAGACGAA TAAGAAGCAC 
ATCCACAGCC GGAGCACCTT GGAATGTGCC TACAGGACGC ACCTGGTTGC TGGGATTGGC 
TTCTACCAGC ATCTGCTGCT CTACATCCAG TCCCACTACC AGCTGGAGCT TCAGTGCTGC 
ATCGACTGGA CCCATGTTAC TGATCCCCTC ATAGGATGCA AGAAGCCAGT GTCTGCATCA 
GGGAAGGAGA TGGACTGGGC ACAGATGGCC TGCCACCGAT GTCTCATGTA CCTGGGGGAT 
TTGTCACGAT ATCAGAATGA ATTAGCTGGC GTAGACACTG AGCTGCTAGC TGAGAGATTT 
TACTACCAAG CCCTGTCTGT AGCTCCCCAG ATCGGAATGC CCTTTAACCA GCTGGGCACC 
CTTGCAGGCA GCAAGTACTA CAACGTGGAA GCCATGTACT GTTACCTGCG CTGCATCCAG 
TCGGAAGTGT CCTTTGAGGG GGCCTATGGG AATCTCAAGC GGCTGTATGA CAAGGCAGCC 
AAGATGTACC ACCAGCTGAA GAAGTGTGAG ACTCGGAAAC TCTCTCCCAG CAAGAAACGC 
TGTAAAGACA TCAAGAGGTT GCTGGTAAAC TTCATGTACC TTCAAAGCCT CCTGCAGCCC 
AGGAGCAGCC CGGTGGACTC AGAGCTGACC TCACTCTGCC AGTCAGTCCT GGAGGACTTC 
AATCTCTGCC TCTTCTACCT GCCATCCTCA CCCAGCCTCA GCCTGGCCAG TGAGGGTGAA 
GAGGACTATG AAAGTGGCTA TGCCTTCCTC CCAGACCTCC TCATCTTTCA GATGATCATC 
ATTTGTCTCA TGGGTGTGCA TAGCTTGAAG AGAGCAGGGT CCAAACAGTA CAGTGCAGCC 
ATTGCCTTTA CCCTGGCCCT CTTCTCCCAC CTCGTCAACC ATGTCAATAT ACGGCTGCAG 
GCTGAGCTGG AGGAGGGTGA GAATCCCGTG CCGGCGTTCC AGAGCGATGG CACAGATGAG 
CCCTTGGAGA AGGAGGAGGC ACCGGGGCCT GAGCCCCCTC CTGCAGTACC TCAAGCGACT 
GAGGTCAGAA AGAGCCGGAA GTTCTCTCGC CTCTCCTGCC TCCGCCGCCG CCGCCACCCA 
CCCAAAGCTG GAGATGACAG TGACCTGAGT GAAGGCTTTG AATCAGACTC GAGCCATGAC 
TCAGCCAGGG CCAGTGAGGG CTCAGACAGT GGCTCCGACA AGAGTCTCGA AGGTGGGGGA 
ACAGCCTTTG ATGCTGAGAC AGACTCAGAA ATGAACAGCC AAGAGTCCCG GTCAGACCTG 
GAAGACATTG AGGATGAGGA GGGTACTCGG TCCCCAGCCC TGGAGCCCCC TCGGCCCAGG 
TCAGAGGCTC CCGAGTCTCT CAATGGCCCA CTGGGCCCCA GTGAGGCGAG CATTGCCAGC 
AATCTACAGG CCATGTCCAC GCAGATGTTC CAGACCAAGC GCTGCTTCCG ACTGGCGCCC 
ACCTTCAGCA ACCTGCTCCT CCAGCCTTCC ACTGAGCCCC ACACTGTAGC CAACCGCAGG 
TCATGTGTCA ATGGAGATGC AGACAAGCCT TCGGAACCAG AACTCTGCTC CAGAGTGCAG 
GCAGGGCGAT TGTGCCCTCC AGCTTGGGGC CGAAGCCCAA TGCCACGTGC CTGCTGTTCA 
TCCCAGCTCT GTCTCCCACT CCCAGCCTCC GAGGAGGGCT CCGAGTCGGA GGGCAGTGAA 
TCTAGTGGGC ACTCCTGTCG GAATGAGCGC AGTGTCCAGG AGAAGCTGCA AGTCCTGGCA 
GCCGAAGGCC TGCTTCCTGC TGTGAAGGTC TTCCTGGACT GGCTGCGGGC CAACCCTGAC 
CTCATCGTTG TGTGTGCACA GAGCTCTCAA AGTCTGTGGA ATCGCCTGTC TGTGTTGCTG 
AACCTGTTGC CAGCTGCTGG TGAGCTCCAA GAGTCTGGCC TGGCCCTGTG TCCCGAGGTC 
CAGGACCTAC TTGAAGGTTG TGAACTGCCT GACCTGCCCT CCAGTCTGCT GCTCCCAGAG 
GACACGGCCC TTCGTAACCT GCCTCCCCTC CGGGCTGCCC ATAGACGCTT CAACTTTGAC 
ACAGACCGGC CCCTGCTTAA CTCCTTAGAG GAGTCAGTCG TGCGCATCTG CTGCATCCGC 
AGCTTTGGAC ACTTCATCGC CCACCTGCAG GGCAGCATCC TGCAGTTCAG CGCAGAGGCT 
GGCATCTTTG TCAGCATCGC CCAGTCTGAG CAGGAGGGCG TGCTGCAGCA GGCCCAGGCG 
CAGTTCCGCA TGGCTCAGGA AGAGGCCCGT CGGAACAGGC TGATGAGAGA CATGGCCCAG 
CTGCGACTTC AGCTCGAGGT CTCTCAGCTG GAAGGGAGCC TGCAGCAGCC CAAGGCCCAG 
TCAGCAATGT CTCCTTACCT CGTCCCGGAC ACCCAGGCCC TCTGCCACCA CCTCCCGGTC 
ATCCGCCAGC TGGCCACCAG CGGCCGCTTC ATTGTCATCA TCCCAAGGAC AGTGATTGAT 
GGCCTGGATT TGCTGAAGAA GGAACACCCA GGGGCCCGGG ATGGGATCCG TTACCTGGAG 
GCAGAGTTTA AGAAAGGAAA CAGGTACATT CGCTGCCAGA AAGAAGTAGG AAAGAGCTTC 
GAGCGGCATA AGCTGAAGAG GCAGGATGCA GATGCCTGGA CGCTCTATAA GATCCTAGAC 
AGCTGCAAAC AGTTGACTTT GGCCCAGGGG GCAGGCGAGG AGGACCCAAG CGGCATGGTG 
ACCATCGTCA CAGGCTTTCC ACTGGACAAC CCCAGTGCGC TCTCAGGCCC CATGCAGGCA 
GCCCTGCAGG CCGCTACACA TGCCAGTGTG GACATCAAGA ATGTTCTGGA CTTCTACAAG 
CAGTGGAAGG AGATTGGTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMG5 ( XM_022421262.1 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_022276970.1 Canis lupus familiaris SMG5, nonsense mediated mRNA decay factor (SMG5), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OCe289689
Clone ID Related Accession (Same CDS sequence)	XM_022421262.1
Accession Version	XM_022421262.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3003bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	protein SMG5 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006589.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCCAAG GCCCCCCCCC AGGGGAGAGC AGCGAGCCCG AGGCGAAGGT CCTCCATACC 
AAGCGGCTTT ACCGGGCTGT GGTGGAGGCT GTGCATCGAC TCGACCTCAT CCTTTGCAAC 
AAAACTGCTT ATCAAGAAGT GTTTAAACCA GAGAACATTA GCCTGAGGAA CAAGCTGCGT 
GAGCTCTGCG TCAAGCTTAT GTTCCTGCAC CCAGTAGACT ATGGGAGAAA GGCTGAGGAG 
CTGCTGTGGA GAAAGGTGTA CTACGAAGTT ATTCAGCTTA TCAAGACGAA TAAGAAGCAC 
ATCCACAGCC GGAGCACCTT GGAATGTGCC TACAGGACGC ACCTGGTTGC TGGGATTGGC 
TTCTACCAGC ATCTGCTGCT CTACATCCAG TCCCACTACC AGCTGGAGCT TCAGTGCTGC 
ATCGACTGGA CCCATGTTAC TGATCCCCTC ATAGGATGCA AGAAGCCAGT GTCTGCATCA 
GGGAAGGAGA TGGACTGGGC ACAGATGGCC TGCCACCGAT GTCTCATGTA CCTGGGGGAT 
TTGTCACGAT ATCAGAATGA ATTAGCTGGC GTAGACACTG AGCTGCTAGC TGAGAGATTT 
TACTACCAAG CCCTGTCTGT AGCTCCCCAG ATCGGAATGC CCTTTAACCA GCTGGGCACC 
CTTGCAGGCA GCAAGTACTA CAACGTGGAA GCCATGTACT GTTACCTGCG CTGCATCCAG 
TCGGAAGTGT CCTTTGAGGG GGCCTATGGG AATCTCAAGC GGCTGTATGA CAAGGCAGCC 
AAGATGTACC ACCAGCTGAA GAAGTGTGAG ACTCGGAAAC TCTCTCCCAG CAAGAAACGC 
TGTAAAGACA TCAAGAGGTT GCTGGTAAAC TTCATGTACC TTCAAAGCCT CCTGCAGCCC 
AGGAGCAGCC CGGTGGACTC AGAGCTGACC TCACTCTGCC AGTCAGTCCT GGAGGACTTC 
AATCTCTGCC TCTTCTACCT GCCATCCTCA CCCAGCCTCA GCCTGGCCAG TGAGGGTGAA 
GAGGACTATG AAAGTGGCTA TGCCTTCCTC CCAGACCTCC TCATCTTTCA GATGATCATC 
ATTTGTCTCA TGGGTGTGCA TAGCTTGAAG AGAGCAGATG AGCCCTTGGA GAAGGAGGAG 
GCACCGGGGC CTGAGCCCCC TCCTGCAGTA CCTCAAGCGA CTGAGGTCAG AAAGAGCCGG 
AAGTTCTCTC GCCTCTCCTG CCTCCGCCGC CGCCGCCACC CACCCAAAGC TGGAGATGAC 
AGTGACCTGA GTGAAGGCTT TGAATCAGAC TCGAGCCATG ACTCAGCCAG GGCCAGTGAG 
GGCTCAGACA GTGGCTCCGA CAAGAGTCTC GAAGGTGGGG GAACAGCCTT TGATGCTGAG 
ACAGACTCAG AAATGAACAG CCAAGAGTCC CGGTCAGACC TGGAAGACAT TGAGGATGAG 
GAGGGTACTC GGTCCCCAGC CCTGGAGCCC CCTCGGCCCA GGTCAGAGGC TCCCGAGTCT 
CTCAATGGCC CACTGGGCCC CAGTGAGGCG AGCATTGCCA GCAATCTACA GGCCATGTCC 
ACGCAGATGT TCCAGACCAA GCGCTGCTTC CGACTGGCGC CCACCTTCAG CAACCTGCTC 
CTCCAGCCTT CCACTGAGCC CCACACTGTA GCCAACCGCA GGTCATGTGT CAATGGAGAT 
GCAGACAAGC CTTCGGAACC AGAACTCTGC TCCAGAGTGC AGGCAGGGCG ATTGTGCCCT 
CCAGCTTGGG GCCGAAGCCC AATGCCACGT GCCTGCTGTT CATCCCAGCT CTGTCTCCCA 
CTCCCAGCCT CCGAGGAGGG CTCCGAGTCG GAGGGCAGTG AATCTAGTGG GCACTCCTGT 
CGGAATGAGC GCAGTGTCCA GGAGAAGCTG CAAGTCCTGG CAGCCGAAGG CCTGCTTCCT 
GCTGTGAAGG TCTTCCTGGA CTGGCTGCGG GCCAACCCTG ACCTCATCGT TGTGTGTGCA 
CAGAGCTCTC AAAGTCTGTG GAATCGCCTG TCTGTGTTGC TGAACCTGTT GCCAGCTGCT 
GGTGAGCTCC AAGAGTCTGG CCTGGCCCTG TGTCCCGAGG TCCAGGACCT ACTTGAAGGT 
TGTGAACTGC CTGACCTGCC CTCCAGTCTG CTGCTCCCAG AGGACACGGC CCTTCGTAAC 
CTGCCTCCCC TCCGGGCTGC CCATAGACGC TTCAACTTTG ACACAGACCG GCCCCTGCTT 
AACTCCTTAG AGGAGTCAGT CGTGCGCATC TGCTGCATCC GCAGCTTTGG ACACTTCATC 
GCCCACCTGC AGGGCAGCAT CCTGCAGTTC AGCGCAGAGG CTGGCATCTT TGTCAGCATC 
GCCCAGTCTG AGCAGGAGGG CGTGCTGCAG CAGGCCCAGG CGCAGTTCCG CATGGCTCAG 
GAAGAGGCCC GTCGGAACAG GCTGATGAGA GACATGGCCC AGCTGCGACT TCAGCTCGAG 
GTCTCTCAGC TGGAAGGGAG CCTGCAGCAG CCCAAGGCCC AGTCAGCAAT GTCTCCTTAC 
CTCGTCCCGG ACACCCAGGC CCTCTGCCAC CACCTCCCGG TCATCCGCCA GCTGGCCACC 
AGCGGCCGCT TCATTGTCAT CATCCCAAGG ACAGTGATTG ATGGCCTGGA TTTGCTGAAG 
AAGGAACACC CAGGGGCCCG GGATGGGATC CGTTACCTGG AGGCAGAGTT TAAGAAAGGA 
AACAGGTACA TTCGCTGCCA GAAAGAAGTA GGAAAGAGCT TCGAGCGGCA TAAGCTGAAG 
AGGCAGGATG CAGATGCCTG GACGCTCTAT AAGATCCTAG ACAGCTGCAA ACAGTTGACT 
TTGGCCCAGG GGGCAGGCGA GGAGGACCCA AGCGGCATGG TGACCATCGT CACAGGCTTT 
CCACTGGACA ACCCCAGTGC GCTCTCAGGC CCCATGCAGG CAGCCCTGCA GGCCGCTACA 
CATGCCAGTG TGGACATCAA GAATGTTCTG GACTTCTACA AGCAGTGGAA GGAGATTGGT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022276970.1
CDS	146..3148
Translation	MSQGPPPGESSEPEAKVLHTKRLYRAVVEAVHRLDLILCNKTAYQEVFKPENISLRNKLRELCVKLMFLHPVDYGRKAEELLWRKVYYEVIQLIKTNKKHIHSRSTLECAYRTHLVAGIGFYQHLLLYIQSHYQLELQCCIDWTHVTDPLIGCKKPVSASGKEMDWAQMACHRCLMYLGDLSRYQNELAGVDTELLAERFYYQALSVAPQIGMPFNQLGTLAGSKYYNVEAMYCYLRCIQSEVSFEGAYGNLKRLYDKAAKMYHQLKKCETRKLSPSKKRCKDIKRLLVNFMYLQSLLQPRSSPVDSELTSLCQSVLEDFNLCLFYLPSSPSLSLASEGEEDYESGYAFLPDLLIFQMIIICLMGVHSLKRADEPLEKEEAPGPEPPPAVPQATEVRKSRKFSRLSCLRRRRHPPKAGDDSDLSEGFESDSSHDSARASEGSDSGSDKSLEGGGTAFDAETDSEMNSQESRSDLEDIEDEEGTRSPALEPPRPRSEAPESLNGPLGPSEASIASNLQAMSTQMFQTKRCFRLAPTFSNLLLQPSTEPHTVANRRSCVNGDADKPSEPELCSRVQAGRLCPPAWGRSPMPRACCSSQLCLPLPASEEGSESEGSESSGHSCRNERSVQEKLQVLAAEGLLPAVKVFLDWLRANPDLIVVCAQSSQSLWNRLSVLLNLLPAAGELQESGLALCPEVQDLLEGCELPDLPSSLLLPEDTALRNLPPLRAAHRRFNFDTDRPLLNSLEESVVRICCIRSFGHFIAHLQGSILQFSAEAGIFVSIAQSEQEGVLQQAQAQFRMAQEEARRNRLMRDMAQLRLQLEVSQLEGSLQQPKAQSAMSPYLVPDTQALCHHLPVIRQLATSGRFIVIIPRTVIDGLDLLKKEHPGARDGIRYLEAEFKKGNRYIRCQKEVGKSFERHKLKRQDADAWTLYKILDSCKQLTLAQGAGEEDPSGMVTIVTGFPLDNPSALSGPMQAALQAATHASVDIKNVLDFYKQWKEIG

Target ORF information:

RefSeq Version	XM_022421262.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris SMG5, nonsense mediated mRNA decay factor (SMG5), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022421262.1

ORF Insert Sequence:

ATGAGCCAAG GCCCCCCCCC AGGGGAGAGC AGCGAGCCCG AGGCGAAGGT CCTCCATACC 
AAGCGGCTTT ACCGGGCTGT GGTGGAGGCT GTGCATCGAC TCGACCTCAT CCTTTGCAAC 
AAAACTGCTT ATCAAGAAGT GTTTAAACCA GAGAACATTA GCCTGAGGAA CAAGCTGCGT 
GAGCTCTGCG TCAAGCTTAT GTTCCTGCAC CCAGTAGACT ATGGGAGAAA GGCTGAGGAG 
CTGCTGTGGA GAAAGGTGTA CTACGAAGTT ATTCAGCTTA TCAAGACGAA TAAGAAGCAC 
ATCCACAGCC GGAGCACCTT GGAATGTGCC TACAGGACGC ACCTGGTTGC TGGGATTGGC 
TTCTACCAGC ATCTGCTGCT CTACATCCAG TCCCACTACC AGCTGGAGCT TCAGTGCTGC 
ATCGACTGGA CCCATGTTAC TGATCCCCTC ATAGGATGCA AGAAGCCAGT GTCTGCATCA 
GGGAAGGAGA TGGACTGGGC ACAGATGGCC TGCCACCGAT GTCTCATGTA CCTGGGGGAT 
TTGTCACGAT ATCAGAATGA ATTAGCTGGC GTAGACACTG AGCTGCTAGC TGAGAGATTT 
TACTACCAAG CCCTGTCTGT AGCTCCCCAG ATCGGAATGC CCTTTAACCA GCTGGGCACC 
CTTGCAGGCA GCAAGTACTA CAACGTGGAA GCCATGTACT GTTACCTGCG CTGCATCCAG 
TCGGAAGTGT CCTTTGAGGG GGCCTATGGG AATCTCAAGC GGCTGTATGA CAAGGCAGCC 
AAGATGTACC ACCAGCTGAA GAAGTGTGAG ACTCGGAAAC TCTCTCCCAG CAAGAAACGC 
TGTAAAGACA TCAAGAGGTT GCTGGTAAAC TTCATGTACC TTCAAAGCCT CCTGCAGCCC 
AGGAGCAGCC CGGTGGACTC AGAGCTGACC TCACTCTGCC AGTCAGTCCT GGAGGACTTC 
AATCTCTGCC TCTTCTACCT GCCATCCTCA CCCAGCCTCA GCCTGGCCAG TGAGGGTGAA 
GAGGACTATG AAAGTGGCTA TGCCTTCCTC CCAGACCTCC TCATCTTTCA GATGATCATC 
ATTTGTCTCA TGGGTGTGCA TAGCTTGAAG AGAGCAGATG AGCCCTTGGA GAAGGAGGAG 
GCACCGGGGC CTGAGCCCCC TCCTGCAGTA CCTCAAGCGA CTGAGGTCAG AAAGAGCCGG 
AAGTTCTCTC GCCTCTCCTG CCTCCGCCGC CGCCGCCACC CACCCAAAGC TGGAGATGAC 
AGTGACCTGA GTGAAGGCTT TGAATCAGAC TCGAGCCATG ACTCAGCCAG GGCCAGTGAG 
GGCTCAGACA GTGGCTCCGA CAAGAGTCTC GAAGGTGGGG GAACAGCCTT TGATGCTGAG 
ACAGACTCAG AAATGAACAG CCAAGAGTCC CGGTCAGACC TGGAAGACAT TGAGGATGAG 
GAGGGTACTC GGTCCCCAGC CCTGGAGCCC CCTCGGCCCA GGTCAGAGGC TCCCGAGTCT 
CTCAATGGCC CACTGGGCCC CAGTGAGGCG AGCATTGCCA GCAATCTACA GGCCATGTCC 
ACGCAGATGT TCCAGACCAA GCGCTGCTTC CGACTGGCGC CCACCTTCAG CAACCTGCTC 
CTCCAGCCTT CCACTGAGCC CCACACTGTA GCCAACCGCA GGTCATGTGT CAATGGAGAT 
GCAGACAAGC CTTCGGAACC AGAACTCTGC TCCAGAGTGC AGGCAGGGCG ATTGTGCCCT 
CCAGCTTGGG GCCGAAGCCC AATGCCACGT GCCTGCTGTT CATCCCAGCT CTGTCTCCCA 
CTCCCAGCCT CCGAGGAGGG CTCCGAGTCG GAGGGCAGTG AATCTAGTGG GCACTCCTGT 
CGGAATGAGC GCAGTGTCCA GGAGAAGCTG CAAGTCCTGG CAGCCGAAGG CCTGCTTCCT 
GCTGTGAAGG TCTTCCTGGA CTGGCTGCGG GCCAACCCTG ACCTCATCGT TGTGTGTGCA 
CAGAGCTCTC AAAGTCTGTG GAATCGCCTG TCTGTGTTGC TGAACCTGTT GCCAGCTGCT 
GGTGAGCTCC AAGAGTCTGG CCTGGCCCTG TGTCCCGAGG TCCAGGACCT ACTTGAAGGT 
TGTGAACTGC CTGACCTGCC CTCCAGTCTG CTGCTCCCAG AGGACACGGC CCTTCGTAAC 
CTGCCTCCCC TCCGGGCTGC CCATAGACGC TTCAACTTTG ACACAGACCG GCCCCTGCTT 
AACTCCTTAG AGGAGTCAGT CGTGCGCATC TGCTGCATCC GCAGCTTTGG ACACTTCATC 
GCCCACCTGC AGGGCAGCAT CCTGCAGTTC AGCGCAGAGG CTGGCATCTT TGTCAGCATC 
GCCCAGTCTG AGCAGGAGGG CGTGCTGCAG CAGGCCCAGG CGCAGTTCCG CATGGCTCAG 
GAAGAGGCCC GTCGGAACAG GCTGATGAGA GACATGGCCC AGCTGCGACT TCAGCTCGAG 
GTCTCTCAGC TGGAAGGGAG CCTGCAGCAG CCCAAGGCCC AGTCAGCAAT GTCTCCTTAC 
CTCGTCCCGG ACACCCAGGC CCTCTGCCAC CACCTCCCGG TCATCCGCCA GCTGGCCACC 
AGCGGCCGCT TCATTGTCAT CATCCCAAGG ACAGTGATTG ATGGCCTGGA TTTGCTGAAG 
AAGGAACACC CAGGGGCCCG GGATGGGATC CGTTACCTGG AGGCAGAGTT TAAGAAAGGA 
AACAGGTACA TTCGCTGCCA GAAAGAAGTA GGAAAGAGCT TCGAGCGGCA TAAGCTGAAG 
AGGCAGGATG CAGATGCCTG GACGCTCTAT AAGATCCTAG ACAGCTGCAA ACAGTTGACT 
TTGGCCCAGG GGGCAGGCGA GGAGGACCCA AGCGGCATGG TGACCATCGT CACAGGCTTT 
CCACTGGACA ACCCCAGTGC GCTCTCAGGC CCCATGCAGG CAGCCCTGCA GGCCGCTACA 
CATGCCAGTG TGGACATCAA GAATGTTCTG GACTTCTACA AGCAGTGGAA GGAGATTGGT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

見積依頼

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03-6811-6572

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03-6811-6573
050-3174-7589(受注専用)

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オンライン注文

SMG5 cDNA ORF clone, Canis lupus familiaris(Dog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

会社情報

サポート

会社情報