SMG5 cDNA ORF clone, Gallus gallus(chicken)

The following SMG5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SMG5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OGa46039 XM_015298318.2
Latest version!
Gallus gallus SMG5, nonsense mediated mRNA decay factor (SMG5), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OGa46040 XM_015298319.2
Latest version!
Gallus gallus SMG5, nonsense mediated mRNA decay factor (SMG5), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
View Old Accession Versions >>
OGa46039 XM_015298318.1 Gallus gallus SMG5 nonsense mediated mRNA decay factor (SMG5), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OGa46040 XM_015298319.1 Gallus gallus SMG5 nonsense mediated mRNA decay factor (SMG5), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OGa46039
    Clone ID Related Accession (Same CDS sequence) XM_015298318.2 , XM_015298318.1
    Accession Version XM_015298318.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3150bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product protein SMG5 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006110.5) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015298318.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGAGCCAGG GCGGCGGGAC GGGCAGCGAG AGCGGAGAGC CTGAGGCCAA AGTGCTGCAC 
    ACCAAACGGC TGTACCGAGC GGTGGTGGAG GCCGTGCATC GCCTGGATCT CATCCTCGGC
    AATAAGGCAG CGTACCAGGA GGTGTTCAAA CCCGAGAACA TCAGCCTGAG GAACAAGCTG
    CGGGAGCTGT GTGTGAAGCT GATGTTCTTG CATCCCGTGG ATTATGGCAG AAAAGCGGAA
    GAGCTGCTCT GGAGAAAGGT TTACTATGAA GTTATCCAAC TCATCAAGAC GAATAAGAAG
    CACATCCACA GCCGCAGCGC TCTGGAGTGC GCCTACCGCA CTCACCTGGT GGCCGGCATT
    GGCTTCTACC AGCACCTGCT GCTCTACATC CAGTCCCACT ACCAGCTGGA GCTGCAGTGC
    TGCATCGACT GGACGCACGT GACGGACCCG CTCATGGGCT GCAAGAAACC CGTGTCTGCA
    TCGGAGAAGG AGATGGAGTG GGCGCAGATG GCGTGCCACC GATGCCTGGT TTACCTTGGG
    GATCTGGCGC GCTACCAGAA CGAGCTGGCG GGTGTGGACA CGGAGCTGCT GGCTGAGCGC
    TTCTACTACC AGGCGCTGTC TGTCGCGCCG CAGATCGGCA TGCCCTTCAA TCAGCTGGGG
    ACGCTGGCTG GGAGCAAATA CTACAACGTG GAGGCCACGT ACTGCTACTT ACGCTGCATT
    CAATCCGAAG TGTCCTTCGA AGGCGCCTCC GGGAACCTGA AGCGGCTGTA CGACAAAGCG
    GCCAAAATGT ACCAGCAGCT GAAGAAGTGT GAGAGCAGGA AGCTGTCCCC CAGCAAGAAG
    CGCTGCAAAG ACATTAAGAG GCTGCTGGTG AGCTTCATGT ACCTGCAGAG CCTCCTGCAG
    CCCAAGAGCA GCTCTCTGGA TTCTGAGCTG ACGTCGCTGT GCCAGTCGGT GCTGGAGGAT
    TTCAACCTGT GCTTGTTCTA CCTGCCTTCC CCTCCCAACC TGAGCTCAGT GAGTGAGGAT
    GAGGAGGAGT ACGAGAGCGG CTACTCCTTC CTTCCCGACC TGCTCATCTT CCGCATGGTG
    ATCATCTGCT TCATGAGCGT TCACAGCCTC AGGCGCGCAG GCTCCAAGCA GTACAGCGCA
    GCCATCGCTT TCACGCTGGC CCTCTTCTCT CACCTCATCA ACCACGTCAA CATTCGGCTG
    CAGGCGGAGC TGGAGGAAGG GGAGAACCCT GTGCCGGCGT TCCGCAGCGA TGGCACAGAT
    GAGCCGGAGC CACGGGAGCC CCTCAGCTCC ATGGAGAAGG AAGGGGAGGC AGAGCTGCCC
    AATCACCACC CCAGGGAGGA GGAACGGAAA CACGACTGCA AGAAAAGCAA GAAATACTCA
    CGGCTTTCCT GCCTGCGCCG CCGCCGGCAC CCGCAGAAGG CGGATGAGAG CGACCTGAGC
    GAGGGCTTCG ACTCTGACTC CAGCCAGGGC TCCACGAAGG GCAGCGACGG CTCCGAGAGC
    GGCTCGGAGA AGAGCGACGA GGAAGCGGAG GCCGCTTTCG ATGTGGAGAC GGACTCTGAC
    ATGAACAGCC AGGAGTCGCG GTCCGACTTG GAGGACATGG AGGACGAGCC GGGCAAGGAG
    GGCGGCGAGC GAGGCCGGAG CGGGGCCGGG GAGGCCCTGA AAAACGGCGT GGACGGCGGC
    GGATCCGGGG AGTGCAAGAA TATGAGCAGC TCCAAAACGG AGAGCGCGGA CCCAGCCGTG
    AACGGGCCCA CGTCCCTGAG CGCCAGCGAC GCCAGCATCG CCAGCAACCT GCAGGCCATG
    TCCACGCAGC TCTTCCAGAC CAAGCGCTGC TTCCGCTTGG CCCCCACCTT CAGCAACATC
    CTCCTGAAGC CCAGCGGTGA GCAGCCCGCC TCGAAGGGCG GCTTGGAGAG CAAGCCCTGC
    ATCAATGGTG ATGTGGAGAA GCCCGGCTTG GCAGAGCAAG ACAACGTGTC TGACTCTGAG
    GAGAGCGTGT TGAGCACCAA GTCGTGCAGG AACGAGCGGT CCCTGCAGGA GAAGCTGGAG
    ATCGTGAGCA ACGAGGGGCT GCTGCTCACC GTCAAGGTGT TCCTGGACTG GCTGAGGACA
    AACACGGACT TCATCATCAT GTGTGCTCAG AGCTCCCAGA GCCTATGGAA CAGACTGTCT
    GTGCTCCTCA ACCTGCTGCC TTCTGCTGCC GACCTGCAGG ATTCGGGGCT GGAGCTGTGC
    AAGGAGGTGA GGGAGGTGCT GAGCGGCGCG GAGCTGCCGG AGCTGCGTGC CAACCTGCTG
    CTCCCCGAGG ACGTGGCCCT GCGAAATCTG CCCCCCCTGA GGAACGCGCA CAGGAGGCTG
    AACTTCGAGC AGGACCGCCC CATCTTCTCG GCACTGGAGG AGTCCGTCGT TCGGATCTGC
    TGCATCCGGA GCTTCGGGCA CTTCGTCACA CGCCTGCAGG GCAGCATCCT GCAGTTCAAC
    CCGGAGCTGG GGATCTTCAT CAGCATAGCT CAGTCGGAGC GGGACAGCGT GGTGCAGCAG
    GCCCAGGCCC AGTTCCACAT GGCTGCAGAG GAGGCTCGTC GGAACCGGCT GATGAGAGAC
    ATGGCTCAGC TCCGCCTGCA GCTGGAGGTG TCTCAGCTGG AGGGCAGCCT GCAGCAGCCC
    AAGGCGCAGT CGGCCATGTC TCCGTACCTC GTGCTGGACG CGCAGGCGCT CTGCCAGCAC
    CTGGCCGTGG TCAAGCAGCT GGCCGGCAGC GGGCGCTTCA TCATCATCAT CCCGCGGGCA
    GTGATCGACA GCTTGGACTT CCTCAAGAAG GAGAACGCCG GCGCCCGCGA CAGCATCCGC
    TACCTGGAGG CGGAGTTTAA AAAGGGAAAC CGGTACATTC GTTGCCAGAG AGATGTTGGG
    AAGAGCTTTG AGAGGCACAA ACTGAAGAGA CAAGACTTGG ATGCGTGGAA CCTCTATAAG
    ATCCTGGACA GCTGCAAGCA GCTGACGGTG TCCCAGAGCA GCGGGGACGA CGACACCACG
    GGAATGGTCA CCATCATCAC GGGCTTCCAG CTGGAGGACC CCGGGGCGCT GTCGGCGCCC
    ATGCAGTCCG CCATCCAAGC GGCCGCCGGC GCCAGCGTGG AGGTGAAGAA CATCCTGGAG
    TTCTACAAGC AGTGGAAAGA GATGGGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015153804.1
    CDS624..3773
    Translation

    Target ORF information:

    RefSeq Version XM_015298318.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus SMG5, nonsense mediated mRNA decay factor (SMG5), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015298318.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGAGCCAGG GCGGCGGGAC GGGCAGCGAG AGCGGAGAGC CTGAGGCCAA AGTGCTGCAC 
    ACCAAACGGC TGTACCGAGC GGTGGTGGAG GCCGTGCATC GCCTGGATCT CATCCTCGGC
    AATAAGGCAG CGTACCAGGA GGTGTTCAAA CCCGAGAACA TCAGCCTGAG GAACAAGCTG
    CGGGAGCTGT GTGTGAAGCT GATGTTCTTG CATCCCGTGG ATTATGGCAG AAAAGCGGAA
    GAGCTGCTCT GGAGAAAGGT TTACTATGAA GTTATCCAAC TCATCAAGAC GAATAAGAAG
    CACATCCACA GCCGCAGCGC TCTGGAGTGC GCCTACCGCA CTCACCTGGT GGCCGGCATT
    GGCTTCTACC AGCACCTGCT GCTCTACATC CAGTCCCACT ACCAGCTGGA GCTGCAGTGC
    TGCATCGACT GGACGCACGT GACGGACCCG CTCATGGGCT GCAAGAAACC CGTGTCTGCA
    TCGGAGAAGG AGATGGAGTG GGCGCAGATG GCGTGCCACC GATGCCTGGT TTACCTTGGG
    GATCTGGCGC GCTACCAGAA CGAGCTGGCG GGTGTGGACA CGGAGCTGCT GGCTGAGCGC
    TTCTACTACC AGGCGCTGTC TGTCGCGCCG CAGATCGGCA TGCCCTTCAA TCAGCTGGGG
    ACGCTGGCTG GGAGCAAATA CTACAACGTG GAGGCCACGT ACTGCTACTT ACGCTGCATT
    CAATCCGAAG TGTCCTTCGA AGGCGCCTCC GGGAACCTGA AGCGGCTGTA CGACAAAGCG
    GCCAAAATGT ACCAGCAGCT GAAGAAGTGT GAGAGCAGGA AGCTGTCCCC CAGCAAGAAG
    CGCTGCAAAG ACATTAAGAG GCTGCTGGTG AGCTTCATGT ACCTGCAGAG CCTCCTGCAG
    CCCAAGAGCA GCTCTCTGGA TTCTGAGCTG ACGTCGCTGT GCCAGTCGGT GCTGGAGGAT
    TTCAACCTGT GCTTGTTCTA CCTGCCTTCC CCTCCCAACC TGAGCTCAGT GAGTGAGGAT
    GAGGAGGAGT ACGAGAGCGG CTACTCCTTC CTTCCCGACC TGCTCATCTT CCGCATGGTG
    ATCATCTGCT TCATGAGCGT TCACAGCCTC AGGCGCGCAG GCTCCAAGCA GTACAGCGCA
    GCCATCGCTT TCACGCTGGC CCTCTTCTCT CACCTCATCA ACCACGTCAA CATTCGGCTG
    CAGGCGGAGC TGGAGGAAGG GGAGAACCCT GTGCCGGCGT TCCGCAGCGA TGGCACAGAT
    GAGCCGGAGC CACGGGAGCC CCTCAGCTCC ATGGAGAAGG AAGGGGAGGC AGAGCTGCCC
    AATCACCACC CCAGGGAGGA GGAACGGAAA CACGACTGCA AGAAAAGCAA GAAATACTCA
    CGGCTTTCCT GCCTGCGCCG CCGCCGGCAC CCGCAGAAGG CGGATGAGAG CGACCTGAGC
    GAGGGCTTCG ACTCTGACTC CAGCCAGGGC TCCACGAAGG GCAGCGACGG CTCCGAGAGC
    GGCTCGGAGA AGAGCGACGA GGAAGCGGAG GCCGCTTTCG ATGTGGAGAC GGACTCTGAC
    ATGAACAGCC AGGAGTCGCG GTCCGACTTG GAGGACATGG AGGACGAGCC GGGCAAGGAG
    GGCGGCGAGC GAGGCCGGAG CGGGGCCGGG GAGGCCCTGA AAAACGGCGT GGACGGCGGC
    GGATCCGGGG AGTGCAAGAA TATGAGCAGC TCCAAAACGG AGAGCGCGGA CCCAGCCGTG
    AACGGGCCCA CGTCCCTGAG CGCCAGCGAC GCCAGCATCG CCAGCAACCT GCAGGCCATG
    TCCACGCAGC TCTTCCAGAC CAAGCGCTGC TTCCGCTTGG CCCCCACCTT CAGCAACATC
    CTCCTGAAGC CCAGCGGTGA GCAGCCCGCC TCGAAGGGCG GCTTGGAGAG CAAGCCCTGC
    ATCAATGGTG ATGTGGAGAA GCCCGGCTTG GCAGAGCAAG ACAACGTGTC TGACTCTGAG
    GAGAGCGTGT TGAGCACCAA GTCGTGCAGG AACGAGCGGT CCCTGCAGGA GAAGCTGGAG
    ATCGTGAGCA ACGAGGGGCT GCTGCTCACC GTCAAGGTGT TCCTGGACTG GCTGAGGACA
    AACACGGACT TCATCATCAT GTGTGCTCAG AGCTCCCAGA GCCTATGGAA CAGACTGTCT
    GTGCTCCTCA ACCTGCTGCC TTCTGCTGCC GACCTGCAGG ATTCGGGGCT GGAGCTGTGC
    AAGGAGGTGA GGGAGGTGCT GAGCGGCGCG GAGCTGCCGG AGCTGCGTGC CAACCTGCTG
    CTCCCCGAGG ACGTGGCCCT GCGAAATCTG CCCCCCCTGA GGAACGCGCA CAGGAGGCTG
    AACTTCGAGC AGGACCGCCC CATCTTCTCG GCACTGGAGG AGTCCGTCGT TCGGATCTGC
    TGCATCCGGA GCTTCGGGCA CTTCGTCACA CGCCTGCAGG GCAGCATCCT GCAGTTCAAC
    CCGGAGCTGG GGATCTTCAT CAGCATAGCT CAGTCGGAGC GGGACAGCGT GGTGCAGCAG
    GCCCAGGCCC AGTTCCACAT GGCTGCAGAG GAGGCTCGTC GGAACCGGCT GATGAGAGAC
    ATGGCTCAGC TCCGCCTGCA GCTGGAGGTG TCTCAGCTGG AGGGCAGCCT GCAGCAGCCC
    AAGGCGCAGT CGGCCATGTC TCCGTACCTC GTGCTGGACG CGCAGGCGCT CTGCCAGCAC
    CTGGCCGTGG TCAAGCAGCT GGCCGGCAGC GGGCGCTTCA TCATCATCAT CCCGCGGGCA
    GTGATCGACA GCTTGGACTT CCTCAAGAAG GAGAACGCCG GCGCCCGCGA CAGCATCCGC
    TACCTGGAGG CGGAGTTTAA AAAGGGAAAC CGGTACATTC GTTGCCAGAG AGATGTTGGG
    AAGAGCTTTG AGAGGCACAA ACTGAAGAGA CAAGACTTGG ATGCGTGGAA CCTCTATAAG
    ATCCTGGACA GCTGCAAGCA GCTGACGGTG TCCCAGAGCA GCGGGGACGA CGACACCACG
    GGAATGGTCA CCATCATCAC GGGCTTCCAG CTGGAGGACC CCGGGGCGCT GTCGGCGCCC
    ATGCAGTCCG CCATCCAAGC GGCCGCCGGC GCCAGCGTGG AGGTGAAGAA CATCCTGGAG
    TTCTACAAGC AGTGGAAAGA GATGGGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa46039
    Clone ID Related Accession (Same CDS sequence) XM_015298318.2 , XM_015298318.1
    Accession Version XM_015298318.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3150bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product protein SMG5 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_456008.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGAGCCAGG GCGGCGGGAC GGGCAGCGAG AGCGGAGAGC CTGAGGCCAA AGTGCTGCAC 
    ACCAAACGGC TGTACCGAGC GGTGGTGGAG GCCGTGCATC GCCTGGATCT CATCCTCGGC
    AATAAGGCAG CGTACCAGGA GGTGTTCAAA CCCGAGAACA TCAGCCTGAG GAACAAGCTG
    CGGGAGCTGT GTGTGAAGCT GATGTTCTTG CATCCCGTGG ATTATGGCAG AAAAGCGGAA
    GAGCTGCTCT GGAGAAAGGT TTACTATGAA GTTATCCAAC TCATCAAGAC GAATAAGAAG
    CACATCCACA GCCGCAGCGC TCTGGAGTGC GCCTACCGCA CTCACCTGGT GGCCGGCATT
    GGCTTCTACC AGCACCTGCT GCTCTACATC CAGTCCCACT ACCAGCTGGA GCTGCAGTGC
    TGCATCGACT GGACGCACGT GACGGACCCG CTCATGGGCT GCAAGAAACC CGTGTCTGCA
    TCGGAGAAGG AGATGGAGTG GGCGCAGATG GCGTGCCACC GATGCCTGGT TTACCTTGGG
    GATCTGGCGC GCTACCAGAA CGAGCTGGCG GGTGTGGACA CGGAGCTGCT GGCTGAGCGC
    TTCTACTACC AGGCGCTGTC TGTCGCGCCG CAGATCGGCA TGCCCTTCAA TCAGCTGGGG
    ACGCTGGCTG GGAGCAAATA CTACAACGTG GAGGCCACGT ACTGCTACTT ACGCTGCATT
    CAATCCGAAG TGTCCTTCGA AGGCGCCTCC GGGAACCTGA AGCGGCTGTA CGACAAAGCG
    GCCAAAATGT ACCAGCAGCT GAAGAAGTGT GAGAGCAGGA AGCTGTCCCC CAGCAAGAAG
    CGCTGCAAAG ACATTAAGAG GCTGCTGGTG AGCTTCATGT ACCTGCAGAG CCTCCTGCAG
    CCCAAGAGCA GCTCTCTGGA TTCTGAGCTG ACGTCGCTGT GCCAGTCGGT GCTGGAGGAT
    TTCAACCTGT GCTTGTTCTA CCTGCCTTCC CCTCCCAACC TGAGCTCAGT GAGTGAGGAT
    GAGGAGGAGT ACGAGAGCGG CTACTCCTTC CTTCCCGACC TGCTCATCTT CCGCATGGTG
    ATCATCTGCT TCATGAGCGT TCACAGCCTC AGGCGCGCAG GCTCCAAGCA GTACAGCGCA
    GCCATCGCTT TCACGCTGGC CCTCTTCTCT CACCTCATCA ACCACGTCAA CATTCGGCTG
    CAGGCGGAGC TGGAGGAAGG GGAGAACCCT GTGCCGGCGT TCCGCAGCGA TGGCACAGAT
    GAGCCGGAGC CACGGGAGCC CCTCAGCTCC ATGGAGAAGG AAGGGGAGGC AGAGCTGCCC
    AATCACCACC CCAGGGAGGA GGAACGGAAA CACGACTGCA AGAAAAGCAA GAAATACTCA
    CGGCTTTCCT GCCTGCGCCG CCGCCGGCAC CCGCAGAAGG CGGATGAGAG CGACCTGAGC
    GAGGGCTTCG ACTCTGACTC CAGCCAGGGC TCCACGAAGG GCAGCGACGG CTCCGAGAGC
    GGCTCGGAGA AGAGCGACGA GGAAGCGGAG GCCGCTTTCG ATGTGGAGAC GGACTCTGAC
    ATGAACAGCC AGGAGTCGCG GTCCGACTTG GAGGACATGG AGGACGAGCC GGGCAAGGAG
    GGCGGCGAGC GAGGCCGGAG CGGGGCCGGG GAGGCCCTGA AAAACGGCGT GGACGGCGGC
    GGATCCGGGG AGTGCAAGAA TATGAGCAGC TCCAAAACGG AGAGCGCGGA CCCAGCCGTG
    AACGGGCCCA CGTCCCTGAG CGCCAGCGAC GCCAGCATCG CCAGCAACCT GCAGGCCATG
    TCCACGCAGC TCTTCCAGAC CAAGCGCTGC TTCCGCTTGG CCCCCACCTT CAGCAACATC
    CTCCTGAAGC CCAGCGGTGA GCAGCCCGCC TCGAAGGGCG GCTTGGAGAG CAAGCCCTGC
    ATCAATGGTG ATGTGGAGAA GCCCGGCTTG GCAGAGCAAG ACAACGTGTC TGACTCTGAG
    GAGAGCGTGT TGAGCACCAA GTCGTGCAGG AACGAGCGGT CCCTGCAGGA GAAGCTGGAG
    ATCGTGAGCA ACGAGGGGCT GCTGCTCACC GTCAAGGTGT TCCTGGACTG GCTGAGGACA
    AACACGGACT TCATCATCAT GTGTGCTCAG AGCTCCCAGA GCCTATGGAA CAGACTGTCT
    GTGCTCCTCA ACCTGCTGCC TTCTGCTGCC GACCTGCAGG ATTCGGGGCT GGAGCTGTGC
    AAGGAGGTGA GGGAGGTGCT GAGCGGCGCG GAGCTGCCGG AGCTGCGTGC CAACCTGCTG
    CTCCCCGAGG ACGTGGCCCT GCGAAATCTG CCCCCCCTGA GGAACGCGCA CAGGAGGCTG
    AACTTCGAGC AGGACCGCCC CATCTTCTCG GCACTGGAGG AGTCCGTCGT TCGGATCTGC
    TGCATCCGGA GCTTCGGGCA CTTCGTCACA CGCCTGCAGG GCAGCATCCT GCAGTTCAAC
    CCGGAGCTGG GGATCTTCAT CAGCATAGCT CAGTCGGAGC GGGACAGCGT GGTGCAGCAG
    GCCCAGGCCC AGTTCCACAT GGCTGCAGAG GAGGCTCGTC GGAACCGGCT GATGAGAGAC
    ATGGCTCAGC TCCGCCTGCA GCTGGAGGTG TCTCAGCTGG AGGGCAGCCT GCAGCAGCCC
    AAGGCGCAGT CGGCCATGTC TCCGTACCTC GTGCTGGACG CGCAGGCGCT CTGCCAGCAC
    CTGGCCGTGG TCAAGCAGCT GGCCGGCAGC GGGCGCTTCA TCATCATCAT CCCGCGGGCA
    GTGATCGACA GCTTGGACTT CCTCAAGAAG GAGAACGCCG GCGCCCGCGA CAGCATCCGC
    TACCTGGAGG CGGAGTTTAA AAAGGGAAAC CGGTACATTC GTTGCCAGAG AGATGTTGGG
    AAGAGCTTTG AGAGGCACAA ACTGAAGAGA CAAGACTTGG ATGCGTGGAA CCTCTATAAG
    ATCCTGGACA GCTGCAAGCA GCTGACGGTG TCCCAGAGCA GCGGGGACGA CGACACCACG
    GGAATGGTCA CCATCATCAC GGGCTTCCAG CTGGAGGACC CCGGGGCGCT GTCGGCGCCC
    ATGCAGTCCG CCATCCAAGC GGCCGCCGGC GCCAGCGTGG AGGTGAAGAA CATCCTGGAG
    TTCTACAAGC AGTGGAAAGA GATGGGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015153804.1
    CDS178..3327
    Translation

    Target ORF information:

    RefSeq Version XM_015298318.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus SMG5 nonsense mediated mRNA decay factor (SMG5), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015298318.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGAGCCAGG GCGGCGGGAC GGGCAGCGAG AGCGGAGAGC CTGAGGCCAA AGTGCTGCAC 
    ACCAAACGGC TGTACCGAGC GGTGGTGGAG GCCGTGCATC GCCTGGATCT CATCCTCGGC
    AATAAGGCAG CGTACCAGGA GGTGTTCAAA CCCGAGAACA TCAGCCTGAG GAACAAGCTG
    CGGGAGCTGT GTGTGAAGCT GATGTTCTTG CATCCCGTGG ATTATGGCAG AAAAGCGGAA
    GAGCTGCTCT GGAGAAAGGT TTACTATGAA GTTATCCAAC TCATCAAGAC GAATAAGAAG
    CACATCCACA GCCGCAGCGC TCTGGAGTGC GCCTACCGCA CTCACCTGGT GGCCGGCATT
    GGCTTCTACC AGCACCTGCT GCTCTACATC CAGTCCCACT ACCAGCTGGA GCTGCAGTGC
    TGCATCGACT GGACGCACGT GACGGACCCG CTCATGGGCT GCAAGAAACC CGTGTCTGCA
    TCGGAGAAGG AGATGGAGTG GGCGCAGATG GCGTGCCACC GATGCCTGGT TTACCTTGGG
    GATCTGGCGC GCTACCAGAA CGAGCTGGCG GGTGTGGACA CGGAGCTGCT GGCTGAGCGC
    TTCTACTACC AGGCGCTGTC TGTCGCGCCG CAGATCGGCA TGCCCTTCAA TCAGCTGGGG
    ACGCTGGCTG GGAGCAAATA CTACAACGTG GAGGCCACGT ACTGCTACTT ACGCTGCATT
    CAATCCGAAG TGTCCTTCGA AGGCGCCTCC GGGAACCTGA AGCGGCTGTA CGACAAAGCG
    GCCAAAATGT ACCAGCAGCT GAAGAAGTGT GAGAGCAGGA AGCTGTCCCC CAGCAAGAAG
    CGCTGCAAAG ACATTAAGAG GCTGCTGGTG AGCTTCATGT ACCTGCAGAG CCTCCTGCAG
    CCCAAGAGCA GCTCTCTGGA TTCTGAGCTG ACGTCGCTGT GCCAGTCGGT GCTGGAGGAT
    TTCAACCTGT GCTTGTTCTA CCTGCCTTCC CCTCCCAACC TGAGCTCAGT GAGTGAGGAT
    GAGGAGGAGT ACGAGAGCGG CTACTCCTTC CTTCCCGACC TGCTCATCTT CCGCATGGTG
    ATCATCTGCT TCATGAGCGT TCACAGCCTC AGGCGCGCAG GCTCCAAGCA GTACAGCGCA
    GCCATCGCTT TCACGCTGGC CCTCTTCTCT CACCTCATCA ACCACGTCAA CATTCGGCTG
    CAGGCGGAGC TGGAGGAAGG GGAGAACCCT GTGCCGGCGT TCCGCAGCGA TGGCACAGAT
    GAGCCGGAGC CACGGGAGCC CCTCAGCTCC ATGGAGAAGG AAGGGGAGGC AGAGCTGCCC
    AATCACCACC CCAGGGAGGA GGAACGGAAA CACGACTGCA AGAAAAGCAA GAAATACTCA
    CGGCTTTCCT GCCTGCGCCG CCGCCGGCAC CCGCAGAAGG CGGATGAGAG CGACCTGAGC
    GAGGGCTTCG ACTCTGACTC CAGCCAGGGC TCCACGAAGG GCAGCGACGG CTCCGAGAGC
    GGCTCGGAGA AGAGCGACGA GGAAGCGGAG GCCGCTTTCG ATGTGGAGAC GGACTCTGAC
    ATGAACAGCC AGGAGTCGCG GTCCGACTTG GAGGACATGG AGGACGAGCC GGGCAAGGAG
    GGCGGCGAGC GAGGCCGGAG CGGGGCCGGG GAGGCCCTGA AAAACGGCGT GGACGGCGGC
    GGATCCGGGG AGTGCAAGAA TATGAGCAGC TCCAAAACGG AGAGCGCGGA CCCAGCCGTG
    AACGGGCCCA CGTCCCTGAG CGCCAGCGAC GCCAGCATCG CCAGCAACCT GCAGGCCATG
    TCCACGCAGC TCTTCCAGAC CAAGCGCTGC TTCCGCTTGG CCCCCACCTT CAGCAACATC
    CTCCTGAAGC CCAGCGGTGA GCAGCCCGCC TCGAAGGGCG GCTTGGAGAG CAAGCCCTGC
    ATCAATGGTG ATGTGGAGAA GCCCGGCTTG GCAGAGCAAG ACAACGTGTC TGACTCTGAG
    GAGAGCGTGT TGAGCACCAA GTCGTGCAGG AACGAGCGGT CCCTGCAGGA GAAGCTGGAG
    ATCGTGAGCA ACGAGGGGCT GCTGCTCACC GTCAAGGTGT TCCTGGACTG GCTGAGGACA
    AACACGGACT TCATCATCAT GTGTGCTCAG AGCTCCCAGA GCCTATGGAA CAGACTGTCT
    GTGCTCCTCA ACCTGCTGCC TTCTGCTGCC GACCTGCAGG ATTCGGGGCT GGAGCTGTGC
    AAGGAGGTGA GGGAGGTGCT GAGCGGCGCG GAGCTGCCGG AGCTGCGTGC CAACCTGCTG
    CTCCCCGAGG ACGTGGCCCT GCGAAATCTG CCCCCCCTGA GGAACGCGCA CAGGAGGCTG
    AACTTCGAGC AGGACCGCCC CATCTTCTCG GCACTGGAGG AGTCCGTCGT TCGGATCTGC
    TGCATCCGGA GCTTCGGGCA CTTCGTCACA CGCCTGCAGG GCAGCATCCT GCAGTTCAAC
    CCGGAGCTGG GGATCTTCAT CAGCATAGCT CAGTCGGAGC GGGACAGCGT GGTGCAGCAG
    GCCCAGGCCC AGTTCCACAT GGCTGCAGAG GAGGCTCGTC GGAACCGGCT GATGAGAGAC
    ATGGCTCAGC TCCGCCTGCA GCTGGAGGTG TCTCAGCTGG AGGGCAGCCT GCAGCAGCCC
    AAGGCGCAGT CGGCCATGTC TCCGTACCTC GTGCTGGACG CGCAGGCGCT CTGCCAGCAC
    CTGGCCGTGG TCAAGCAGCT GGCCGGCAGC GGGCGCTTCA TCATCATCAT CCCGCGGGCA
    GTGATCGACA GCTTGGACTT CCTCAAGAAG GAGAACGCCG GCGCCCGCGA CAGCATCCGC
    TACCTGGAGG CGGAGTTTAA AAAGGGAAAC CGGTACATTC GTTGCCAGAG AGATGTTGGG
    AAGAGCTTTG AGAGGCACAA ACTGAAGAGA CAAGACTTGG ATGCGTGGAA CCTCTATAAG
    ATCCTGGACA GCTGCAAGCA GCTGACGGTG TCCCAGAGCA GCGGGGACGA CGACACCACG
    GGAATGGTCA CCATCATCAC GGGCTTCCAG CTGGAGGACC CCGGGGCGCT GTCGGCGCCC
    ATGCAGTCCG CCATCCAAGC GGCCGCCGGC GCCAGCGTGG AGGTGAAGAA CATCCTGGAG
    TTCTACAAGC AGTGGAAAGA GATGGGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa46040
    Clone ID Related Accession (Same CDS sequence) XM_015298319.2 , XM_015298319.1
    Accession Version XM_015298319.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3123bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product protein SMG5 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006110.5) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015298319.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGAACGGTA CCGGAGCATC TCTGCCTGAG CACGGAGGTG GAGGGATGAG AGCGGTGGTG 
    GAGGCCGTGC ATCGCCTGGA TCTCATCCTC GGCAATAAGG CAGCGTACCA GGAGGTGTTC
    AAACCCGAGA ACATCAGCCT GAGGAACAAG CTGCGGGAGC TGTGTGTGAA GCTGATGTTC
    TTGCATCCCG TGGATTATGG CAGAAAAGCG GAAGAGCTGC TCTGGAGAAA GGTTTACTAT
    GAAGTTATCC AACTCATCAA GACGAATAAG AAGCACATCC ACAGCCGCAG CGCTCTGGAG
    TGCGCCTACC GCACTCACCT GGTGGCCGGC ATTGGCTTCT ACCAGCACCT GCTGCTCTAC
    ATCCAGTCCC ACTACCAGCT GGAGCTGCAG TGCTGCATCG ACTGGACGCA CGTGACGGAC
    CCGCTCATGG GCTGCAAGAA ACCCGTGTCT GCATCGGAGA AGGAGATGGA GTGGGCGCAG
    ATGGCGTGCC ACCGATGCCT GGTTTACCTT GGGGATCTGG CGCGCTACCA GAACGAGCTG
    GCGGGTGTGG ACACGGAGCT GCTGGCTGAG CGCTTCTACT ACCAGGCGCT GTCTGTCGCG
    CCGCAGATCG GCATGCCCTT CAATCAGCTG GGGACGCTGG CTGGGAGCAA ATACTACAAC
    GTGGAGGCCA CGTACTGCTA CTTACGCTGC ATTCAATCCG AAGTGTCCTT CGAAGGCGCC
    TCCGGGAACC TGAAGCGGCT GTACGACAAA GCGGCCAAAA TGTACCAGCA GCTGAAGAAG
    TGTGAGAGCA GGAAGCTGTC CCCCAGCAAG AAGCGCTGCA AAGACATTAA GAGGCTGCTG
    GTGAGCTTCA TGTACCTGCA GAGCCTCCTG CAGCCCAAGA GCAGCTCTCT GGATTCTGAG
    CTGACGTCGC TGTGCCAGTC GGTGCTGGAG GATTTCAACC TGTGCTTGTT CTACCTGCCT
    TCCCCTCCCA ACCTGAGCTC AGTGAGTGAG GATGAGGAGG AGTACGAGAG CGGCTACTCC
    TTCCTTCCCG ACCTGCTCAT CTTCCGCATG GTGATCATCT GCTTCATGAG CGTTCACAGC
    CTCAGGCGCG CAGGCTCCAA GCAGTACAGC GCAGCCATCG CTTTCACGCT GGCCCTCTTC
    TCTCACCTCA TCAACCACGT CAACATTCGG CTGCAGGCGG AGCTGGAGGA AGGGGAGAAC
    CCTGTGCCGG CGTTCCGCAG CGATGGCACA GATGAGCCGG AGCCACGGGA GCCCCTCAGC
    TCCATGGAGA AGGAAGGGGA GGCAGAGCTG CCCAATCACC ACCCCAGGGA GGAGGAACGG
    AAACACGACT GCAAGAAAAG CAAGAAATAC TCACGGCTTT CCTGCCTGCG CCGCCGCCGG
    CACCCGCAGA AGGCGGATGA GAGCGACCTG AGCGAGGGCT TCGACTCTGA CTCCAGCCAG
    GGCTCCACGA AGGGCAGCGA CGGCTCCGAG AGCGGCTCGG AGAAGAGCGA CGAGGAAGCG
    GAGGCCGCTT TCGATGTGGA GACGGACTCT GACATGAACA GCCAGGAGTC GCGGTCCGAC
    TTGGAGGACA TGGAGGACGA GCCGGGCAAG GAGGGCGGCG AGCGAGGCCG GAGCGGGGCC
    GGGGAGGCCC TGAAAAACGG CGTGGACGGC GGCGGATCCG GGGAGTGCAA GAATATGAGC
    AGCTCCAAAA CGGAGAGCGC GGACCCAGCC GTGAACGGGC CCACGTCCCT GAGCGCCAGC
    GACGCCAGCA TCGCCAGCAA CCTGCAGGCC ATGTCCACGC AGCTCTTCCA GACCAAGCGC
    TGCTTCCGCT TGGCCCCCAC CTTCAGCAAC ATCCTCCTGA AGCCCAGCGG TGAGCAGCCC
    GCCTCGAAGG GCGGCTTGGA GAGCAAGCCC TGCATCAATG GTGATGTGGA GAAGCCCGGC
    TTGGCAGAGC AAGACAACGT GTCTGACTCT GAGGAGAGCG TGTTGAGCAC CAAGTCGTGC
    AGGAACGAGC GGTCCCTGCA GGAGAAGCTG GAGATCGTGA GCAACGAGGG GCTGCTGCTC
    ACCGTCAAGG TGTTCCTGGA CTGGCTGAGG ACAAACACGG ACTTCATCAT CATGTGTGCT
    CAGAGCTCCC AGAGCCTATG GAACAGACTG TCTGTGCTCC TCAACCTGCT GCCTTCTGCT
    GCCGACCTGC AGGATTCGGG GCTGGAGCTG TGCAAGGAGG TGAGGGAGGT GCTGAGCGGC
    GCGGAGCTGC CGGAGCTGCG TGCCAACCTG CTGCTCCCCG AGGACGTGGC CCTGCGAAAT
    CTGCCCCCCC TGAGGAACGC GCACAGGAGG CTGAACTTCG AGCAGGACCG CCCCATCTTC
    TCGGCACTGG AGGAGTCCGT CGTTCGGATC TGCTGCATCC GGAGCTTCGG GCACTTCGTC
    ACACGCCTGC AGGGCAGCAT CCTGCAGTTC AACCCGGAGC TGGGGATCTT CATCAGCATA
    GCTCAGTCGG AGCGGGACAG CGTGGTGCAG CAGGCCCAGG CCCAGTTCCA CATGGCTGCA
    GAGGAGGCTC GTCGGAACCG GCTGATGAGA GACATGGCTC AGCTCCGCCT GCAGCTGGAG
    GTGTCTCAGC TGGAGGGCAG CCTGCAGCAG CCCAAGGCGC AGTCGGCCAT GTCTCCGTAC
    CTCGTGCTGG ACGCGCAGGC GCTCTGCCAG CACCTGGCCG TGGTCAAGCA GCTGGCCGGC
    AGCGGGCGCT TCATCATCAT CATCCCGCGG GCAGTGATCG ACAGCTTGGA CTTCCTCAAG
    AAGGAGAACG CCGGCGCCCG CGACAGCATC CGCTACCTGG AGGCGGAGTT TAAAAAGGGA
    AACCGGTACA TTCGTTGCCA GAGAGATGTT GGGAAGAGCT TTGAGAGGCA CAAACTGAAG
    AGACAAGACT TGGATGCGTG GAACCTCTAT AAGATCCTGG ACAGCTGCAA GCAGCTGACG
    GTGTCCCAGA GCAGCGGGGA CGACGACACC ACGGGAATGG TCACCATCAT CACGGGCTTC
    CAGCTGGAGG ACCCCGGGGC GCTGTCGGCG CCCATGCAGT CCGCCATCCA AGCGGCCGCC
    GGCGCCAGCG TGGAGGTGAA GAACATCCTG GAGTTCTACA AGCAGTGGAA AGAGATGGGC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015153805.1
    CDS316..3438
    Translation

    Target ORF information:

    RefSeq Version XM_015298319.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus SMG5, nonsense mediated mRNA decay factor (SMG5), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015298319.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGAACGGTA CCGGAGCATC TCTGCCTGAG CACGGAGGTG GAGGGATGAG AGCGGTGGTG 
    GAGGCCGTGC ATCGCCTGGA TCTCATCCTC GGCAATAAGG CAGCGTACCA GGAGGTGTTC
    AAACCCGAGA ACATCAGCCT GAGGAACAAG CTGCGGGAGC TGTGTGTGAA GCTGATGTTC
    TTGCATCCCG TGGATTATGG CAGAAAAGCG GAAGAGCTGC TCTGGAGAAA GGTTTACTAT
    GAAGTTATCC AACTCATCAA GACGAATAAG AAGCACATCC ACAGCCGCAG CGCTCTGGAG
    TGCGCCTACC GCACTCACCT GGTGGCCGGC ATTGGCTTCT ACCAGCACCT GCTGCTCTAC
    ATCCAGTCCC ACTACCAGCT GGAGCTGCAG TGCTGCATCG ACTGGACGCA CGTGACGGAC
    CCGCTCATGG GCTGCAAGAA ACCCGTGTCT GCATCGGAGA AGGAGATGGA GTGGGCGCAG
    ATGGCGTGCC ACCGATGCCT GGTTTACCTT GGGGATCTGG CGCGCTACCA GAACGAGCTG
    GCGGGTGTGG ACACGGAGCT GCTGGCTGAG CGCTTCTACT ACCAGGCGCT GTCTGTCGCG
    CCGCAGATCG GCATGCCCTT CAATCAGCTG GGGACGCTGG CTGGGAGCAA ATACTACAAC
    GTGGAGGCCA CGTACTGCTA CTTACGCTGC ATTCAATCCG AAGTGTCCTT CGAAGGCGCC
    TCCGGGAACC TGAAGCGGCT GTACGACAAA GCGGCCAAAA TGTACCAGCA GCTGAAGAAG
    TGTGAGAGCA GGAAGCTGTC CCCCAGCAAG AAGCGCTGCA AAGACATTAA GAGGCTGCTG
    GTGAGCTTCA TGTACCTGCA GAGCCTCCTG CAGCCCAAGA GCAGCTCTCT GGATTCTGAG
    CTGACGTCGC TGTGCCAGTC GGTGCTGGAG GATTTCAACC TGTGCTTGTT CTACCTGCCT
    TCCCCTCCCA ACCTGAGCTC AGTGAGTGAG GATGAGGAGG AGTACGAGAG CGGCTACTCC
    TTCCTTCCCG ACCTGCTCAT CTTCCGCATG GTGATCATCT GCTTCATGAG CGTTCACAGC
    CTCAGGCGCG CAGGCTCCAA GCAGTACAGC GCAGCCATCG CTTTCACGCT GGCCCTCTTC
    TCTCACCTCA TCAACCACGT CAACATTCGG CTGCAGGCGG AGCTGGAGGA AGGGGAGAAC
    CCTGTGCCGG CGTTCCGCAG CGATGGCACA GATGAGCCGG AGCCACGGGA GCCCCTCAGC
    TCCATGGAGA AGGAAGGGGA GGCAGAGCTG CCCAATCACC ACCCCAGGGA GGAGGAACGG
    AAACACGACT GCAAGAAAAG CAAGAAATAC TCACGGCTTT CCTGCCTGCG CCGCCGCCGG
    CACCCGCAGA AGGCGGATGA GAGCGACCTG AGCGAGGGCT TCGACTCTGA CTCCAGCCAG
    GGCTCCACGA AGGGCAGCGA CGGCTCCGAG AGCGGCTCGG AGAAGAGCGA CGAGGAAGCG
    GAGGCCGCTT TCGATGTGGA GACGGACTCT GACATGAACA GCCAGGAGTC GCGGTCCGAC
    TTGGAGGACA TGGAGGACGA GCCGGGCAAG GAGGGCGGCG AGCGAGGCCG GAGCGGGGCC
    GGGGAGGCCC TGAAAAACGG CGTGGACGGC GGCGGATCCG GGGAGTGCAA GAATATGAGC
    AGCTCCAAAA CGGAGAGCGC GGACCCAGCC GTGAACGGGC CCACGTCCCT GAGCGCCAGC
    GACGCCAGCA TCGCCAGCAA CCTGCAGGCC ATGTCCACGC AGCTCTTCCA GACCAAGCGC
    TGCTTCCGCT TGGCCCCCAC CTTCAGCAAC ATCCTCCTGA AGCCCAGCGG TGAGCAGCCC
    GCCTCGAAGG GCGGCTTGGA GAGCAAGCCC TGCATCAATG GTGATGTGGA GAAGCCCGGC
    TTGGCAGAGC AAGACAACGT GTCTGACTCT GAGGAGAGCG TGTTGAGCAC CAAGTCGTGC
    AGGAACGAGC GGTCCCTGCA GGAGAAGCTG GAGATCGTGA GCAACGAGGG GCTGCTGCTC
    ACCGTCAAGG TGTTCCTGGA CTGGCTGAGG ACAAACACGG ACTTCATCAT CATGTGTGCT
    CAGAGCTCCC AGAGCCTATG GAACAGACTG TCTGTGCTCC TCAACCTGCT GCCTTCTGCT
    GCCGACCTGC AGGATTCGGG GCTGGAGCTG TGCAAGGAGG TGAGGGAGGT GCTGAGCGGC
    GCGGAGCTGC CGGAGCTGCG TGCCAACCTG CTGCTCCCCG AGGACGTGGC CCTGCGAAAT
    CTGCCCCCCC TGAGGAACGC GCACAGGAGG CTGAACTTCG AGCAGGACCG CCCCATCTTC
    TCGGCACTGG AGGAGTCCGT CGTTCGGATC TGCTGCATCC GGAGCTTCGG GCACTTCGTC
    ACACGCCTGC AGGGCAGCAT CCTGCAGTTC AACCCGGAGC TGGGGATCTT CATCAGCATA
    GCTCAGTCGG AGCGGGACAG CGTGGTGCAG CAGGCCCAGG CCCAGTTCCA CATGGCTGCA
    GAGGAGGCTC GTCGGAACCG GCTGATGAGA GACATGGCTC AGCTCCGCCT GCAGCTGGAG
    GTGTCTCAGC TGGAGGGCAG CCTGCAGCAG CCCAAGGCGC AGTCGGCCAT GTCTCCGTAC
    CTCGTGCTGG ACGCGCAGGC GCTCTGCCAG CACCTGGCCG TGGTCAAGCA GCTGGCCGGC
    AGCGGGCGCT TCATCATCAT CATCCCGCGG GCAGTGATCG ACAGCTTGGA CTTCCTCAAG
    AAGGAGAACG CCGGCGCCCG CGACAGCATC CGCTACCTGG AGGCGGAGTT TAAAAAGGGA
    AACCGGTACA TTCGTTGCCA GAGAGATGTT GGGAAGAGCT TTGAGAGGCA CAAACTGAAG
    AGACAAGACT TGGATGCGTG GAACCTCTAT AAGATCCTGG ACAGCTGCAA GCAGCTGACG
    GTGTCCCAGA GCAGCGGGGA CGACGACACC ACGGGAATGG TCACCATCAT CACGGGCTTC
    CAGCTGGAGG ACCCCGGGGC GCTGTCGGCG CCCATGCAGT CCGCCATCCA AGCGGCCGCC
    GGCGCCAGCG TGGAGGTGAA GAACATCCTG GAGTTCTACA AGCAGTGGAA AGAGATGGGC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa46040
    Clone ID Related Accession (Same CDS sequence) XM_015298319.2 , XM_015298319.1
    Accession Version XM_015298319.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3123bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product protein SMG5 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_456008.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGAACGGTA CCGGAGCATC TCTGCCTGAG CACGGAGGTG GAGGGATGAG AGCGGTGGTG 
    GAGGCCGTGC ATCGCCTGGA TCTCATCCTC GGCAATAAGG CAGCGTACCA GGAGGTGTTC
    AAACCCGAGA ACATCAGCCT GAGGAACAAG CTGCGGGAGC TGTGTGTGAA GCTGATGTTC
    TTGCATCCCG TGGATTATGG CAGAAAAGCG GAAGAGCTGC TCTGGAGAAA GGTTTACTAT
    GAAGTTATCC AACTCATCAA GACGAATAAG AAGCACATCC ACAGCCGCAG CGCTCTGGAG
    TGCGCCTACC GCACTCACCT GGTGGCCGGC ATTGGCTTCT ACCAGCACCT GCTGCTCTAC
    ATCCAGTCCC ACTACCAGCT GGAGCTGCAG TGCTGCATCG ACTGGACGCA CGTGACGGAC
    CCGCTCATGG GCTGCAAGAA ACCCGTGTCT GCATCGGAGA AGGAGATGGA GTGGGCGCAG
    ATGGCGTGCC ACCGATGCCT GGTTTACCTT GGGGATCTGG CGCGCTACCA GAACGAGCTG
    GCGGGTGTGG ACACGGAGCT GCTGGCTGAG CGCTTCTACT ACCAGGCGCT GTCTGTCGCG
    CCGCAGATCG GCATGCCCTT CAATCAGCTG GGGACGCTGG CTGGGAGCAA ATACTACAAC
    GTGGAGGCCA CGTACTGCTA CTTACGCTGC ATTCAATCCG AAGTGTCCTT CGAAGGCGCC
    TCCGGGAACC TGAAGCGGCT GTACGACAAA GCGGCCAAAA TGTACCAGCA GCTGAAGAAG
    TGTGAGAGCA GGAAGCTGTC CCCCAGCAAG AAGCGCTGCA AAGACATTAA GAGGCTGCTG
    GTGAGCTTCA TGTACCTGCA GAGCCTCCTG CAGCCCAAGA GCAGCTCTCT GGATTCTGAG
    CTGACGTCGC TGTGCCAGTC GGTGCTGGAG GATTTCAACC TGTGCTTGTT CTACCTGCCT
    TCCCCTCCCA ACCTGAGCTC AGTGAGTGAG GATGAGGAGG AGTACGAGAG CGGCTACTCC
    TTCCTTCCCG ACCTGCTCAT CTTCCGCATG GTGATCATCT GCTTCATGAG CGTTCACAGC
    CTCAGGCGCG CAGGCTCCAA GCAGTACAGC GCAGCCATCG CTTTCACGCT GGCCCTCTTC
    TCTCACCTCA TCAACCACGT CAACATTCGG CTGCAGGCGG AGCTGGAGGA AGGGGAGAAC
    CCTGTGCCGG CGTTCCGCAG CGATGGCACA GATGAGCCGG AGCCACGGGA GCCCCTCAGC
    TCCATGGAGA AGGAAGGGGA GGCAGAGCTG CCCAATCACC ACCCCAGGGA GGAGGAACGG
    AAACACGACT GCAAGAAAAG CAAGAAATAC TCACGGCTTT CCTGCCTGCG CCGCCGCCGG
    CACCCGCAGA AGGCGGATGA GAGCGACCTG AGCGAGGGCT TCGACTCTGA CTCCAGCCAG
    GGCTCCACGA AGGGCAGCGA CGGCTCCGAG AGCGGCTCGG AGAAGAGCGA CGAGGAAGCG
    GAGGCCGCTT TCGATGTGGA GACGGACTCT GACATGAACA GCCAGGAGTC GCGGTCCGAC
    TTGGAGGACA TGGAGGACGA GCCGGGCAAG GAGGGCGGCG AGCGAGGCCG GAGCGGGGCC
    GGGGAGGCCC TGAAAAACGG CGTGGACGGC GGCGGATCCG GGGAGTGCAA GAATATGAGC
    AGCTCCAAAA CGGAGAGCGC GGACCCAGCC GTGAACGGGC CCACGTCCCT GAGCGCCAGC
    GACGCCAGCA TCGCCAGCAA CCTGCAGGCC ATGTCCACGC AGCTCTTCCA GACCAAGCGC
    TGCTTCCGCT TGGCCCCCAC CTTCAGCAAC ATCCTCCTGA AGCCCAGCGG TGAGCAGCCC
    GCCTCGAAGG GCGGCTTGGA GAGCAAGCCC TGCATCAATG GTGATGTGGA GAAGCCCGGC
    TTGGCAGAGC AAGACAACGT GTCTGACTCT GAGGAGAGCG TGTTGAGCAC CAAGTCGTGC
    AGGAACGAGC GGTCCCTGCA GGAGAAGCTG GAGATCGTGA GCAACGAGGG GCTGCTGCTC
    ACCGTCAAGG TGTTCCTGGA CTGGCTGAGG ACAAACACGG ACTTCATCAT CATGTGTGCT
    CAGAGCTCCC AGAGCCTATG GAACAGACTG TCTGTGCTCC TCAACCTGCT GCCTTCTGCT
    GCCGACCTGC AGGATTCGGG GCTGGAGCTG TGCAAGGAGG TGAGGGAGGT GCTGAGCGGC
    GCGGAGCTGC CGGAGCTGCG TGCCAACCTG CTGCTCCCCG AGGACGTGGC CCTGCGAAAT
    CTGCCCCCCC TGAGGAACGC GCACAGGAGG CTGAACTTCG AGCAGGACCG CCCCATCTTC
    TCGGCACTGG AGGAGTCCGT CGTTCGGATC TGCTGCATCC GGAGCTTCGG GCACTTCGTC
    ACACGCCTGC AGGGCAGCAT CCTGCAGTTC AACCCGGAGC TGGGGATCTT CATCAGCATA
    GCTCAGTCGG AGCGGGACAG CGTGGTGCAG CAGGCCCAGG CCCAGTTCCA CATGGCTGCA
    GAGGAGGCTC GTCGGAACCG GCTGATGAGA GACATGGCTC AGCTCCGCCT GCAGCTGGAG
    GTGTCTCAGC TGGAGGGCAG CCTGCAGCAG CCCAAGGCGC AGTCGGCCAT GTCTCCGTAC
    CTCGTGCTGG ACGCGCAGGC GCTCTGCCAG CACCTGGCCG TGGTCAAGCA GCTGGCCGGC
    AGCGGGCGCT TCATCATCAT CATCCCGCGG GCAGTGATCG ACAGCTTGGA CTTCCTCAAG
    AAGGAGAACG CCGGCGCCCG CGACAGCATC CGCTACCTGG AGGCGGAGTT TAAAAAGGGA
    AACCGGTACA TTCGTTGCCA GAGAGATGTT GGGAAGAGCT TTGAGAGGCA CAAACTGAAG
    AGACAAGACT TGGATGCGTG GAACCTCTAT AAGATCCTGG ACAGCTGCAA GCAGCTGACG
    GTGTCCCAGA GCAGCGGGGA CGACGACACC ACGGGAATGG TCACCATCAT CACGGGCTTC
    CAGCTGGAGG ACCCCGGGGC GCTGTCGGCG CCCATGCAGT CCGCCATCCA AGCGGCCGCC
    GGCGCCAGCG TGGAGGTGAA GAACATCCTG GAGTTCTACA AGCAGTGGAA AGAGATGGGC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015153805.1
    CDS317..3439
    Translation

    Target ORF information:

    RefSeq Version XM_015298319.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus SMG5 nonsense mediated mRNA decay factor (SMG5), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015298319.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGAACGGTA CCGGAGCATC TCTGCCTGAG CACGGAGGTG GAGGGATGAG AGCGGTGGTG 
    GAGGCCGTGC ATCGCCTGGA TCTCATCCTC GGCAATAAGG CAGCGTACCA GGAGGTGTTC
    AAACCCGAGA ACATCAGCCT GAGGAACAAG CTGCGGGAGC TGTGTGTGAA GCTGATGTTC
    TTGCATCCCG TGGATTATGG CAGAAAAGCG GAAGAGCTGC TCTGGAGAAA GGTTTACTAT
    GAAGTTATCC AACTCATCAA GACGAATAAG AAGCACATCC ACAGCCGCAG CGCTCTGGAG
    TGCGCCTACC GCACTCACCT GGTGGCCGGC ATTGGCTTCT ACCAGCACCT GCTGCTCTAC
    ATCCAGTCCC ACTACCAGCT GGAGCTGCAG TGCTGCATCG ACTGGACGCA CGTGACGGAC
    CCGCTCATGG GCTGCAAGAA ACCCGTGTCT GCATCGGAGA AGGAGATGGA GTGGGCGCAG
    ATGGCGTGCC ACCGATGCCT GGTTTACCTT GGGGATCTGG CGCGCTACCA GAACGAGCTG
    GCGGGTGTGG ACACGGAGCT GCTGGCTGAG CGCTTCTACT ACCAGGCGCT GTCTGTCGCG
    CCGCAGATCG GCATGCCCTT CAATCAGCTG GGGACGCTGG CTGGGAGCAA ATACTACAAC
    GTGGAGGCCA CGTACTGCTA CTTACGCTGC ATTCAATCCG AAGTGTCCTT CGAAGGCGCC
    TCCGGGAACC TGAAGCGGCT GTACGACAAA GCGGCCAAAA TGTACCAGCA GCTGAAGAAG
    TGTGAGAGCA GGAAGCTGTC CCCCAGCAAG AAGCGCTGCA AAGACATTAA GAGGCTGCTG
    GTGAGCTTCA TGTACCTGCA GAGCCTCCTG CAGCCCAAGA GCAGCTCTCT GGATTCTGAG
    CTGACGTCGC TGTGCCAGTC GGTGCTGGAG GATTTCAACC TGTGCTTGTT CTACCTGCCT
    TCCCCTCCCA ACCTGAGCTC AGTGAGTGAG GATGAGGAGG AGTACGAGAG CGGCTACTCC
    TTCCTTCCCG ACCTGCTCAT CTTCCGCATG GTGATCATCT GCTTCATGAG CGTTCACAGC
    CTCAGGCGCG CAGGCTCCAA GCAGTACAGC GCAGCCATCG CTTTCACGCT GGCCCTCTTC
    TCTCACCTCA TCAACCACGT CAACATTCGG CTGCAGGCGG AGCTGGAGGA AGGGGAGAAC
    CCTGTGCCGG CGTTCCGCAG CGATGGCACA GATGAGCCGG AGCCACGGGA GCCCCTCAGC
    TCCATGGAGA AGGAAGGGGA GGCAGAGCTG CCCAATCACC ACCCCAGGGA GGAGGAACGG
    AAACACGACT GCAAGAAAAG CAAGAAATAC TCACGGCTTT CCTGCCTGCG CCGCCGCCGG
    CACCCGCAGA AGGCGGATGA GAGCGACCTG AGCGAGGGCT TCGACTCTGA CTCCAGCCAG
    GGCTCCACGA AGGGCAGCGA CGGCTCCGAG AGCGGCTCGG AGAAGAGCGA CGAGGAAGCG
    GAGGCCGCTT TCGATGTGGA GACGGACTCT GACATGAACA GCCAGGAGTC GCGGTCCGAC
    TTGGAGGACA TGGAGGACGA GCCGGGCAAG GAGGGCGGCG AGCGAGGCCG GAGCGGGGCC
    GGGGAGGCCC TGAAAAACGG CGTGGACGGC GGCGGATCCG GGGAGTGCAA GAATATGAGC
    AGCTCCAAAA CGGAGAGCGC GGACCCAGCC GTGAACGGGC CCACGTCCCT GAGCGCCAGC
    GACGCCAGCA TCGCCAGCAA CCTGCAGGCC ATGTCCACGC AGCTCTTCCA GACCAAGCGC
    TGCTTCCGCT TGGCCCCCAC CTTCAGCAAC ATCCTCCTGA AGCCCAGCGG TGAGCAGCCC
    GCCTCGAAGG GCGGCTTGGA GAGCAAGCCC TGCATCAATG GTGATGTGGA GAAGCCCGGC
    TTGGCAGAGC AAGACAACGT GTCTGACTCT GAGGAGAGCG TGTTGAGCAC CAAGTCGTGC
    AGGAACGAGC GGTCCCTGCA GGAGAAGCTG GAGATCGTGA GCAACGAGGG GCTGCTGCTC
    ACCGTCAAGG TGTTCCTGGA CTGGCTGAGG ACAAACACGG ACTTCATCAT CATGTGTGCT
    CAGAGCTCCC AGAGCCTATG GAACAGACTG TCTGTGCTCC TCAACCTGCT GCCTTCTGCT
    GCCGACCTGC AGGATTCGGG GCTGGAGCTG TGCAAGGAGG TGAGGGAGGT GCTGAGCGGC
    GCGGAGCTGC CGGAGCTGCG TGCCAACCTG CTGCTCCCCG AGGACGTGGC CCTGCGAAAT
    CTGCCCCCCC TGAGGAACGC GCACAGGAGG CTGAACTTCG AGCAGGACCG CCCCATCTTC
    TCGGCACTGG AGGAGTCCGT CGTTCGGATC TGCTGCATCC GGAGCTTCGG GCACTTCGTC
    ACACGCCTGC AGGGCAGCAT CCTGCAGTTC AACCCGGAGC TGGGGATCTT CATCAGCATA
    GCTCAGTCGG AGCGGGACAG CGTGGTGCAG CAGGCCCAGG CCCAGTTCCA CATGGCTGCA
    GAGGAGGCTC GTCGGAACCG GCTGATGAGA GACATGGCTC AGCTCCGCCT GCAGCTGGAG
    GTGTCTCAGC TGGAGGGCAG CCTGCAGCAG CCCAAGGCGC AGTCGGCCAT GTCTCCGTAC
    CTCGTGCTGG ACGCGCAGGC GCTCTGCCAG CACCTGGCCG TGGTCAAGCA GCTGGCCGGC
    AGCGGGCGCT TCATCATCAT CATCCCGCGG GCAGTGATCG ACAGCTTGGA CTTCCTCAAG
    AAGGAGAACG CCGGCGCCCG CGACAGCATC CGCTACCTGG AGGCGGAGTT TAAAAAGGGA
    AACCGGTACA TTCGTTGCCA GAGAGATGTT GGGAAGAGCT TTGAGAGGCA CAAACTGAAG
    AGACAAGACT TGGATGCGTG GAACCTCTAT AAGATCCTGG ACAGCTGCAA GCAGCTGACG
    GTGTCCCAGA GCAGCGGGGA CGACGACACC ACGGGAATGG TCACCATCAT CACGGGCTTC
    CAGCTGGAGG ACCCCGGGGC GCTGTCGGCG CCCATGCAGT CCGCCATCCA AGCGGCCGCC
    GGCGCCAGCG TGGAGGTGAA GAACATCCTG GAGTTCTACA AGCAGTGGAA AGAGATGGGC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.