SMG5 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	SMG5
Entrez Gene ID	513598
Full Name	SMG5, nonsense mediated mRNA decay factor
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_024988339.1	XP_024844107.1	protein SMG5 isoform X1
NM_001083679.1	NP_001077148.1	protein SMG5

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	SMG5	XP_513880.3
	SMG5	XP_002801899.1
Canis lupus familiaris (dog)	SMG5	XP_547535.3
Bos taurus (cattle)	SMG5	NP_001077148.1
Rattus norvegicus (Norway rat)	Smg5	XP_001059906.3
Gallus gallus (chicken)	SMG5	XP_423228.4
Xenopus tropicalis (tropical clawed frog)	smg5	NP_001072622.1
Homo sapiens (human)	SMG5	NP_056142.2
Mus musculus (house mouse)	Smg5	NP_839977.2

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following SMG5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SMG5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBa232728	XM_024988339.1 Latest version!	Bos taurus SMG5, nonsense mediated mRNA decay factor (SMG5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OBa36427	NM_001083679.1 Latest version!	Bos taurus SMG5 nonsense mediated mRNA decay factor (SMG5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

ORF Online Only Promotion

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SMG5 ( XM_024988339.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024844107.1 Bos taurus SMG5, nonsense mediated mRNA decay factor (SMG5), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa232728
Clone ID Related Accession (Same CDS sequence)	XM_024988339.1
Accession Version	XM_024988339.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2562bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	protein SMG5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTGGG CACAGATGGC ATGCCACCGA TGTCTGGTGT ACCTGGGGGA TTTGTCACGG 
TATCAGAATG AACTAGCTGG CGTAGACACT GAGCTGCTAG CTGAGAGATT TTACTACCAA 
GCCCTGTCAG TAGCTCCTCA GATTGGAATG CCCTTCAACC AGCTGGGCAC CCTTGCAGGC 
AGCAAGTACT ATAATGTGGA AGCCATGTAC TGCTACCTGC GCTGCATTCA GTCGGAAGTG 
TCCTTTGAGG GGGCCTATGG AAACCTCAAG CGGCTGTATG ACAAGGCAGC AAAAATGTAC 
CACCAGCTGA AGAAGTGTGA GACTCGGAAA CTCTCTCCCA GCAAGAAGCG ATGTAAAGAC 
ATTAAGAGGT TGCTGGTGAA CTTTATGTAT CTGCAAAGCC TCCTGCAGCC CAAAAGCAGC 
TCCGTGGACT CAGAGCTGAC ATCACTTTGC CAGTCAGTCC TGGAGGACTT CAACCTCTGT 
CTCTTCTACC TGCCCTCCTC ACCCAACCTC AGCCTGGCCA GTGAGGATGA GGAGGAGTAT 
GAGAGTGGAT ATGCTTTCCT CCCAGATCTT CTCATCTTTC AAATGGTCAT CATCTGCCTT 
ATGGGGGTGC ACAGCTTGAA GAGAGCAGGA TCCAAGCAGT ACAGTGCAGC CATTGCCTTC 
ACCCTGGCCC TCTTCTCCCA CCTCGTCAAT CACGTCAACA TCCGGCTGCA AGCTGAGCTG 
GAAGAGGGCG AGAATCCCGT CCCGGCGTTC CAGAGTGATG GCACAGATGA ACCGGAGTCC 
AGGGAACCCT TGGAGAAGGA GGAGGAGCCG GGGCCTGAGC CTCCTCCTGT AGTACCTCCA 
GAGGGTGAGG TCAGAAAGAG TCGGAAGTTC TCCCGCCTCT CCTGCCTCCG CCGCCGCCGC 
CACCCACCCA AAGCAGGTGA TGACAGTGAC CTGAGTGAAG GCTTTGACTC GGACTCAAGC 
CATGACTCGG CCCGGGCCAG TGAGGGCTCG GACAGTGGCT CCGACAAGAG TCTTGAAGGT 
GGGGGAACAG CTTTTGACGC TGAGACAGAT TCAGAAATGA ACAGTCAAGA GTCCCGATCA 
GACCTGGAAG ATATGGAGGA TGAGGACGGG ACACGGTCCC CAGCCCTGGA GCCATCTCGG 
GCCAGGTCAG AGGCTCCTGA ATCCCTCAAT GGGCCTCTGG GCCCCAGCGA GGCCAGCATT 
GCCAGCAATC TACAAGCCAT GTCCACCCAG ATGTTCCAGA CCAAACGCTG CTTCCGACTG 
GCCCCCACCT TCAGCAACCT GCTCCTCCAG CCCACCACTG AAGCACCCGT CTCGGCCACC 
CTCAGGCGCT GTGTCAATGG AGATGTGGAC AAGCCTTCAG AGCCAGCCTC TGAAGAGGGC 
TCGGAGTCGG AGGGAAGCGA GTCCAGTGGG CGCTCCTTTC GGAACGAGCG GAGCATCCAG 
GAGAAGCTGC AGGTCTTGAT GGCCGAAGGC CTGCTCCCTG CTGTGAAAGT CTTCCTGGAC 
TGGCTGCGGA CCAACCCCGA CCTCATCGTT GTGTGTGCGC AGAGCTCTCA AAGTCTGTGG 
AACCGCCTGT CTGTGTTGTT GAACCTGTTG CCAGCTGCTG GTGAACTCCA AGAATCTGGC 
CTGGCCCTGT GTGCTGAGGT CCAGGAGCTG CTTGAAGGCT GTGAACTGCC TGACCTCCCA 
TCTAGCCTGC TGCTCCCAGA GGACACGGCC CTTCGCAACC TGCCTCCCCT CCGGGCGGCC 
CACAGACGCT TTAGCTTTGA TACAGCTCGG CTCTTGCTCA GCACCTTAGA GGAGACAGTC 
GTGCGGATCT GCTGCATACG CAGCTTTGGC CACTTCGTTG CCCGCCTGCA AGGCAGCATC 
CTGCAGTTCA ACGCAGAGGT CGGCATCTTT GTCAGCATCG CCCAGTCTGA GCAGGAGAGC 
CTGCTGCAGC AGGCCCAGGC CCAGTTCCGC ATGGCACAGG AGGAAGCTCG GCGGAACAGG 
CTGATGAGAG ACATGGCCCA GCTACGACTT CAGCTTGAGG TCTCTCAGCT GGAAGGGAGC 
CTGCAGCAGC CCAAGGCACA GTCAGCAATG TCTCCCTACC TCGTCCCCGA CACCCAGGCC 
CTCTGCCACC ACCTCCCTGT CATCCGCCAG CTGGCCACCA GTGGCCGCTT CATCGTCATC 
ATCCCAAGGA CAGTGATCGA TGGCCTGGAT TTGCTGAAGA AGGAACACCC AGGGGCCCGG 
GATGGGATCC GATACCTGGA GGCAGAGTTT AAAAAAGGGA ACAGGTACAT TCGCTGCCAG 
AGAGAGGCGG GGAAGAGCTT TGAGCGGCAT AAGCTGAAGA GGCAGGATGC CGATGCCTGG 
ACGCTCTATA AGATCCTGGA CAGCTGCAAA CAGCTGACTC TGGCCCAGGG AGCAGGTGAG 
GAGGACCCGA GCGGCATGGT GACCATCATC ACAGGCCTTC CACTGGACAA TCCTAGCACG 
CTCTCAGGCC CTATGCAGGC AGCCTTGCAG GCCGCTGCAC ATGCCAGTGT GGACATCAAG 
AATGTTCTGG ACTTCTACAA GCAGTGGAAG GAGATTGGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024844107.1
CDS	58..2619
Translation	MDWAQMACHRCLVYLGDLSRYQNELAGVDTELLAERFYYQALSVAPQIGMPFNQLGTLAGSKYYNVEAMYCYLRCIQSEVSFEGAYGNLKRLYDKAAKMYHQLKKCETRKLSPSKKRCKDIKRLLVNFMYLQSLLQPKSSSVDSELTSLCQSVLEDFNLCLFYLPSSPNLSLASEDEEEYESGYAFLPDLLIFQMVIICLMGVHSLKRAGSKQYSAAIAFTLALFSHLVNHVNIRLQAELEEGENPVPAFQSDGTDEPESREPLEKEEEPGPEPPPVVPPEGEVRKSRKFSRLSCLRRRRHPPKAGDDSDLSEGFDSDSSHDSARASEGSDSGSDKSLEGGGTAFDAETDSEMNSQESRSDLEDMEDEDGTRSPALEPSRARSEAPESLNGPLGPSEASIASNLQAMSTQMFQTKRCFRLAPTFSNLLLQPTTEAPVSATLRRCVNGDVDKPSEPASEEGSESEGSESSGRSFRNERSIQEKLQVLMAEGLLPAVKVFLDWLRTNPDLIVVCAQSSQSLWNRLSVLLNLLPAAGELQESGLALCAEVQELLEGCELPDLPSSLLLPEDTALRNLPPLRAAHRRFSFDTARLLLSTLEETVVRICCIRSFGHFVARLQGSILQFNAEVGIFVSIAQSEQESLLQQAQAQFRMAQEEARRNRLMRDMAQLRLQLEVSQLEGSLQQPKAQSAMSPYLVPDTQALCHHLPVIRQLATSGRFIVIIPRTVIDGLDLLKKEHPGARDGIRYLEAEFKKGNRYIRCQREAGKSFERHKLKRQDADAWTLYKILDSCKQLTLAQGAGEEDPSGMVTIITGLPLDNPSTLSGPMQAALQAAAHASVDIKNVLDFYKQWKEIG

Target ORF information:

RefSeq Version	XM_024988339.1
Organism	Bos taurus(cattle)
Definition	Bos taurus SMG5, nonsense mediated mRNA decay factor (SMG5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024988339.1

ORF Insert Sequence:

ATGGATTGGG CACAGATGGC ATGCCACCGA TGTCTGGTGT ACCTGGGGGA TTTGTCACGG 
TATCAGAATG AACTAGCTGG CGTAGACACT GAGCTGCTAG CTGAGAGATT TTACTACCAA 
GCCCTGTCAG TAGCTCCTCA GATTGGAATG CCCTTCAACC AGCTGGGCAC CCTTGCAGGC 
AGCAAGTACT ATAATGTGGA AGCCATGTAC TGCTACCTGC GCTGCATTCA GTCGGAAGTG 
TCCTTTGAGG GGGCCTATGG AAACCTCAAG CGGCTGTATG ACAAGGCAGC AAAAATGTAC 
CACCAGCTGA AGAAGTGTGA GACTCGGAAA CTCTCTCCCA GCAAGAAGCG ATGTAAAGAC 
ATTAAGAGGT TGCTGGTGAA CTTTATGTAT CTGCAAAGCC TCCTGCAGCC CAAAAGCAGC 
TCCGTGGACT CAGAGCTGAC ATCACTTTGC CAGTCAGTCC TGGAGGACTT CAACCTCTGT 
CTCTTCTACC TGCCCTCCTC ACCCAACCTC AGCCTGGCCA GTGAGGATGA GGAGGAGTAT 
GAGAGTGGAT ATGCTTTCCT CCCAGATCTT CTCATCTTTC AAATGGTCAT CATCTGCCTT 
ATGGGGGTGC ACAGCTTGAA GAGAGCAGGA TCCAAGCAGT ACAGTGCAGC CATTGCCTTC 
ACCCTGGCCC TCTTCTCCCA CCTCGTCAAT CACGTCAACA TCCGGCTGCA AGCTGAGCTG 
GAAGAGGGCG AGAATCCCGT CCCGGCGTTC CAGAGTGATG GCACAGATGA ACCGGAGTCC 
AGGGAACCCT TGGAGAAGGA GGAGGAGCCG GGGCCTGAGC CTCCTCCTGT AGTACCTCCA 
GAGGGTGAGG TCAGAAAGAG TCGGAAGTTC TCCCGCCTCT CCTGCCTCCG CCGCCGCCGC 
CACCCACCCA AAGCAGGTGA TGACAGTGAC CTGAGTGAAG GCTTTGACTC GGACTCAAGC 
CATGACTCGG CCCGGGCCAG TGAGGGCTCG GACAGTGGCT CCGACAAGAG TCTTGAAGGT 
GGGGGAACAG CTTTTGACGC TGAGACAGAT TCAGAAATGA ACAGTCAAGA GTCCCGATCA 
GACCTGGAAG ATATGGAGGA TGAGGACGGG ACACGGTCCC CAGCCCTGGA GCCATCTCGG 
GCCAGGTCAG AGGCTCCTGA ATCCCTCAAT GGGCCTCTGG GCCCCAGCGA GGCCAGCATT 
GCCAGCAATC TACAAGCCAT GTCCACCCAG ATGTTCCAGA CCAAACGCTG CTTCCGACTG 
GCCCCCACCT TCAGCAACCT GCTCCTCCAG CCCACCACTG AAGCACCCGT CTCGGCCACC 
CTCAGGCGCT GTGTCAATGG AGATGTGGAC AAGCCTTCAG AGCCAGCCTC TGAAGAGGGC 
TCGGAGTCGG AGGGAAGCGA GTCCAGTGGG CGCTCCTTTC GGAACGAGCG GAGCATCCAG 
GAGAAGCTGC AGGTCTTGAT GGCCGAAGGC CTGCTCCCTG CTGTGAAAGT CTTCCTGGAC 
TGGCTGCGGA CCAACCCCGA CCTCATCGTT GTGTGTGCGC AGAGCTCTCA AAGTCTGTGG 
AACCGCCTGT CTGTGTTGTT GAACCTGTTG CCAGCTGCTG GTGAACTCCA AGAATCTGGC 
CTGGCCCTGT GTGCTGAGGT CCAGGAGCTG CTTGAAGGCT GTGAACTGCC TGACCTCCCA 
TCTAGCCTGC TGCTCCCAGA GGACACGGCC CTTCGCAACC TGCCTCCCCT CCGGGCGGCC 
CACAGACGCT TTAGCTTTGA TACAGCTCGG CTCTTGCTCA GCACCTTAGA GGAGACAGTC 
GTGCGGATCT GCTGCATACG CAGCTTTGGC CACTTCGTTG CCCGCCTGCA AGGCAGCATC 
CTGCAGTTCA ACGCAGAGGT CGGCATCTTT GTCAGCATCG CCCAGTCTGA GCAGGAGAGC 
CTGCTGCAGC AGGCCCAGGC CCAGTTCCGC ATGGCACAGG AGGAAGCTCG GCGGAACAGG 
CTGATGAGAG ACATGGCCCA GCTACGACTT CAGCTTGAGG TCTCTCAGCT GGAAGGGAGC 
CTGCAGCAGC CCAAGGCACA GTCAGCAATG TCTCCCTACC TCGTCCCCGA CACCCAGGCC 
CTCTGCCACC ACCTCCCTGT CATCCGCCAG CTGGCCACCA GTGGCCGCTT CATCGTCATC 
ATCCCAAGGA CAGTGATCGA TGGCCTGGAT TTGCTGAAGA AGGAACACCC AGGGGCCCGG 
GATGGGATCC GATACCTGGA GGCAGAGTTT AAAAAAGGGA ACAGGTACAT TCGCTGCCAG 
AGAGAGGCGG GGAAGAGCTT TGAGCGGCAT AAGCTGAAGA GGCAGGATGC CGATGCCTGG 
ACGCTCTATA AGATCCTGGA CAGCTGCAAA CAGCTGACTC TGGCCCAGGG AGCAGGTGAG 
GAGGACCCGA GCGGCATGGT GACCATCATC ACAGGCCTTC CACTGGACAA TCCTAGCACG 
CTCTCAGGCC CTATGCAGGC AGCCTTGCAG GCCGCTGCAC ATGCCAGTGT GGACATCAAG 
AATGTTCTGG ACTTCTACAA GCAGTGGAAG GAGATTGGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMG5 ( NM_001083679.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001077148.1 Bos taurus SMG5 nonsense mediated mRNA decay factor (SMG5), mRNA.

Price & Availability↑

CloneID	OBa36427
Clone ID Related Accession (Same CDS sequence)	NM_001083679.1
Accession Version	NM_001083679.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3051bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-29
Organism	Bos taurus(cattle)
Product	protein SMG5
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC134680.1. On Apr 5, 2007 this sequence version replaced XM_591302.3. ##Evidence-Data-START## Transcript exon combination :: BC134680.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145412, SAMN03145413 [ECO:0000348] ##Evidence-Data-END##

ATGAGCCAAG GCCCCCCCAC AGGGGAGAGC AGCGAGCCTG AAGCAAAGGT CCTCCACACT 
AAGCGGCTTT ACCGGGCTGT GGTGGAGGCT GTGCATCGAC TTGACCTCAT CCTTTGCAAC 
AAAACCGCTT ATCAAGAAGT GTTCAAACCG GAGAACATTA GCCTGAGGAA CAAGCTGCGT 
GAGCTCTGCG TCAAGCTTAT GTTCCTGCAC CCGGTGGACT ATGGGAGGAA GGCTGAGGAG 
CTACTGTGGA GAAAGGTATA CTACGAAGTT ATCCAGCTTA TCAAGACTAA CAAAAAGCAT 
ATCCACAGCC GGAGTACCTT GGAATGTGCC TACAGGACAC ACCTGGTTGC TGGTATTGGC 
TTCTACCAGC ATCTCCTTCT CTATATCCAG TCTCACTACC AGCTGGAACT GCAATGCTGC 
ATCGACTGGA CCCATGTCAC CGACCCCCTC ATAGGATGCA AGAAGCCAGT GTCTGCCTCA 
GGGAAGGAGA TGGATTGGGC ACAGATGGCA TGCCACCGAT GTCTGGTGTA CCTGGGGGAT 
TTGTCACGGT ATCAGAATGA ACTAGCTGGC GTAGACACTG AGCTGCTAGC TGAGAGATTT 
TACTACCAAG CCCTGTCAGT AGCTCCTCAG ATTGGAATGC CCTTCAACCA GCTGGGCACC 
CTTGCAGGCA GCAAGTACTA TAATGTGGAA GCCATGTACT GCTACCTGCG CTGCATTCAG 
TCGGAAGTGT CCTTTGAGGG GGCCTATGGA AACCTCAAGC GGCTGTATGA CAAGGCAGCA 
AAAATGTACC ACCAGCTGAA GAAGTGTGAG ACTCGGAAAC TCTCTCCCAG CAAGAAGCGA 
TGTAAAGACA TTAAGAGGTT GCTGGTGAAC TTTATGTATC TGCAAAGCCT CCTGCAGCCC 
AAAAGCAGCT CCGTGGACTC AGAGCTGACA TCACTTTGCC AGTCAGTCCT GGAGGACTTC 
AACCTCTGTC TCTTCTACCT GCCCTCCTCA CCCAACCTCA GCCTGGCCAG TGAGGATGAG 
GAGGAGTATG AGAGTGGATA TGCTTTCCTC CCAGATCTTC TCATCTTTCA AATGGTCATC 
ATCTGCCTTA TGGGGGTGCA CAGCTTGAAG AGAGCAGGAT CCAAGCAGTA CAGTGCAGCC 
ATTGCCTTCA CCCTGGCCCT CTTCTCCCAC CTCGTCAATC ACGTCAACAT CCGGCTGCAA 
GCTGAGCTGG AAGAGGGCGA GAATCCCGTC CCGGCGTTCC AGAGTGATGG CACAGATGAA 
CCGGAGTCCA GGGAACCCTT GGAGAAGGAG GAGGAGCCGG GGCCTGAGCC TCCTCCTGTA 
GTACCTCCAG AGGGTGAGGT CAGAAAGAGT CGGAAGTTCT CCCGCCTCTC CTGCCTCCGC 
CGCCGCCGCC ACCCACCCAA AGCAGGTGAT GACAGTGACC TGAGTGAAGG CTTTGACTCG 
GACTCAAGCC ATGACTCGGC CCGGGCCAGT GAGGGCTCGG ACAGTGGCTC CGACAAGAGT 
CTTGAAGGTG GGGGAACAGC TTTTGACGCT GAGACAGATT CAGAAATGAA CAGTCAAGAG 
TCCCGATCAG ACCTGGAAGA TATGGAGGAT GAGGACGGGA CACGGTCCCC AGCCCTGGAG 
CCATCTCGGG CCAGGTCAGA GGCTCCTGAA TCCCTCAATG GGCCTCTGGG CCCCAGCGAG 
GCCAGCATTG CCAGCAATCT ACAAGCCATG TCCACCCAGA TGTTCCAGAC CAAACGCTGC 
TTCCGACTGG CCCCCACCTT CAGCAACCTG CTCCTCCAGC CCACCACTGA AGCACCCGTC 
TCGGCCACCC TCAGGCGCTG TGTCAATGGA GATGTGGACA AGCCTTCAGA GCCAGCCTCT 
GAAGAGGGCT CGGAGTCGGA GGGAAGCGAG TCCAGTGGGC GCTCCTTTCG GAACGAGCGG 
AGCATCCAGG AGAAGCTGCA GGTCTTGATG GCCGAAGGCC TGCTCCCTGC TGTGAAAGTC 
TTCCTGGACT GGCTGCGGAC CAACCCCGAC CTCATCGTTG TGTGTGCGCA GAGCTCTCAA 
AGTCTGTGGA ACCGCCTGTC TGTGTTGTTG AACCTGTTGC CAGCTGCTGG TGAACTCCAA 
GAATCTGGCC TGGCCCTGTG TGCTGAGGTC CAGGAGCTGC TTGAAGGCTG TGAACTGCCT 
GACCTCCCAT CTAGCCTGCT GCTCCCAGAG GACACGGCCC TTCGCAACCT GCCTCCCCTC 
CGGGCGGCCC ACAGACGCTT TAGCTTTGAT ACAGCTCGGC TCTTGCTCAG CACCTTAGAG 
GAGACAGTCG TGCGGATCTG CTGCATACGC AGCTTTGGCC ACTTCGTTGC CCGCCTGCAA 
GGCAGCATCC TGCAGTTCAA CGCAGAGGTC GGCATCTTTG TCAGCATCGC CCAGTCTGAG 
CAGGAGAGCC TGCTGCAGCA GGCCCAGGCC CAGTTCCGCA TGGCACAGGA GGAAGCTCGG 
CGGAACAGGC TGATGAGAGA CATGGCCCAG CTACGACTTC AGCTTGAGGT CTCTCAGCTG 
GAAGGGAGCC TGCAGCAGCC CAAGGCACAG TCAGCAATGT CTCCCTACCT CGTCCCCGAC 
ACCCAGGCCC TCTGCCACCA CCTCCCTGTC ATCCGCCAGC TGGCCACCAG TGGCCGCTTC 
ATCGTCATCA TCCCAAGGAC AGTGATCGAT GGCCTGGATT TGCTGAAGAA GGAACACCCA 
GGGGCCCGGG ATGGGATCCG ATACCTGGAG GCAGAGTTTA AAAAAGGGAA CAGGTACATT 
CGCTGCCAGA GAGAGGCGGG GAAGAGCTTT GAGCGGCATA AGCTGAAGAG GCAGGATGCC 
GATGCCTGGA CGCTCTATAA GATCCTGGAC AGCTGCAAAC AGCTGACTCT GGCCCAGGGA 
GCAGGTGAGG AGGACCCGAG CGGCATGGTG ACCATCATCA CAGGCCTTCC ACTGGACAAT 
CCTAGCACGC TCTCAGGCCC TATGCAGGCA GCCTTGCAGG CCGCTGCACA TGCCAGTGTG 
GACATCAAGA ATGTTCTGGA CTTCTACAAG CAGTGGAAGG AGATTGGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001077148.1
CDS	127..3177
Translation	MSQGPPTGESSEPEAKVLHTKRLYRAVVEAVHRLDLILCNKTAYQEVFKPENISLRNKLRELCVKLMFLHPVDYGRKAEELLWRKVYYEVIQLIKTNKKHIHSRSTLECAYRTHLVAGIGFYQHLLLYIQSHYQLELQCCIDWTHVTDPLIGCKKPVSASGKEMDWAQMACHRCLVYLGDLSRYQNELAGVDTELLAERFYYQALSVAPQIGMPFNQLGTLAGSKYYNVEAMYCYLRCIQSEVSFEGAYGNLKRLYDKAAKMYHQLKKCETRKLSPSKKRCKDIKRLLVNFMYLQSLLQPKSSSVDSELTSLCQSVLEDFNLCLFYLPSSPNLSLASEDEEEYESGYAFLPDLLIFQMVIICLMGVHSLKRAGSKQYSAAIAFTLALFSHLVNHVNIRLQAELEEGENPVPAFQSDGTDEPESREPLEKEEEPGPEPPPVVPPEGEVRKSRKFSRLSCLRRRRHPPKAGDDSDLSEGFDSDSSHDSARASEGSDSGSDKSLEGGGTAFDAETDSEMNSQESRSDLEDMEDEDGTRSPALEPSRARSEAPESLNGPLGPSEASIASNLQAMSTQMFQTKRCFRLAPTFSNLLLQPTTEAPVSATLRRCVNGDVDKPSEPASEEGSESEGSESSGRSFRNERSIQEKLQVLMAEGLLPAVKVFLDWLRTNPDLIVVCAQSSQSLWNRLSVLLNLLPAAGELQESGLALCAEVQELLEGCELPDLPSSLLLPEDTALRNLPPLRAAHRRFSFDTARLLLSTLEETVVRICCIRSFGHFVARLQGSILQFNAEVGIFVSIAQSEQESLLQQAQAQFRMAQEEARRNRLMRDMAQLRLQLEVSQLEGSLQQPKAQSAMSPYLVPDTQALCHHLPVIRQLATSGRFIVIIPRTVIDGLDLLKKEHPGARDGIRYLEAEFKKGNRYIRCQREAGKSFERHKLKRQDADAWTLYKILDSCKQLTLAQGAGEEDPSGMVTIITGLPLDNPSTLSGPMQAALQAAAHASVDIKNVLDFYKQWKEIG

Target ORF information:

RefSeq Version	NM_001083679.1
Organism	Bos taurus(cattle)
Definition	Bos taurus SMG5 nonsense mediated mRNA decay factor (SMG5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001083679.1

ORF Insert Sequence:

ATGAGCCAAG GCCCCCCCAC AGGGGAGAGC AGCGAGCCTG AAGCAAAGGT CCTCCACACT 
AAGCGGCTTT ACCGGGCTGT GGTGGAGGCT GTGCATCGAC TTGACCTCAT CCTTTGCAAC 
AAAACCGCTT ATCAAGAAGT GTTCAAACCG GAGAACATTA GCCTGAGGAA CAAGCTGCGT 
GAGCTCTGCG TCAAGCTTAT GTTCCTGCAC CCGGTGGACT ATGGGAGGAA GGCTGAGGAG 
CTACTGTGGA GAAAGGTATA CTACGAAGTT ATCCAGCTTA TCAAGACTAA CAAAAAGCAT 
ATCCACAGCC GGAGTACCTT GGAATGTGCC TACAGGACAC ACCTGGTTGC TGGTATTGGC 
TTCTACCAGC ATCTCCTTCT CTATATCCAG TCTCACTACC AGCTGGAACT GCAATGCTGC 
ATCGACTGGA CCCATGTCAC CGACCCCCTC ATAGGATGCA AGAAGCCAGT GTCTGCCTCA 
GGGAAGGAGA TGGATTGGGC ACAGATGGCA TGCCACCGAT GTCTGGTGTA CCTGGGGGAT 
TTGTCACGGT ATCAGAATGA ACTAGCTGGC GTAGACACTG AGCTGCTAGC TGAGAGATTT 
TACTACCAAG CCCTGTCAGT AGCTCCTCAG ATTGGAATGC CCTTCAACCA GCTGGGCACC 
CTTGCAGGCA GCAAGTACTA TAATGTGGAA GCCATGTACT GCTACCTGCG CTGCATTCAG 
TCGGAAGTGT CCTTTGAGGG GGCCTATGGA AACCTCAAGC GGCTGTATGA CAAGGCAGCA 
AAAATGTACC ACCAGCTGAA GAAGTGTGAG ACTCGGAAAC TCTCTCCCAG CAAGAAGCGA 
TGTAAAGACA TTAAGAGGTT GCTGGTGAAC TTTATGTATC TGCAAAGCCT CCTGCAGCCC 
AAAAGCAGCT CCGTGGACTC AGAGCTGACA TCACTTTGCC AGTCAGTCCT GGAGGACTTC 
AACCTCTGTC TCTTCTACCT GCCCTCCTCA CCCAACCTCA GCCTGGCCAG TGAGGATGAG 
GAGGAGTATG AGAGTGGATA TGCTTTCCTC CCAGATCTTC TCATCTTTCA AATGGTCATC 
ATCTGCCTTA TGGGGGTGCA CAGCTTGAAG AGAGCAGGAT CCAAGCAGTA CAGTGCAGCC 
ATTGCCTTCA CCCTGGCCCT CTTCTCCCAC CTCGTCAATC ACGTCAACAT CCGGCTGCAA 
GCTGAGCTGG AAGAGGGCGA GAATCCCGTC CCGGCGTTCC AGAGTGATGG CACAGATGAA 
CCGGAGTCCA GGGAACCCTT GGAGAAGGAG GAGGAGCCGG GGCCTGAGCC TCCTCCTGTA 
GTACCTCCAG AGGGTGAGGT CAGAAAGAGT CGGAAGTTCT CCCGCCTCTC CTGCCTCCGC 
CGCCGCCGCC ACCCACCCAA AGCAGGTGAT GACAGTGACC TGAGTGAAGG CTTTGACTCG 
GACTCAAGCC ATGACTCGGC CCGGGCCAGT GAGGGCTCGG ACAGTGGCTC CGACAAGAGT 
CTTGAAGGTG GGGGAACAGC TTTTGACGCT GAGACAGATT CAGAAATGAA CAGTCAAGAG 
TCCCGATCAG ACCTGGAAGA TATGGAGGAT GAGGACGGGA CACGGTCCCC AGCCCTGGAG 
CCATCTCGGG CCAGGTCAGA GGCTCCTGAA TCCCTCAATG GGCCTCTGGG CCCCAGCGAG 
GCCAGCATTG CCAGCAATCT ACAAGCCATG TCCACCCAGA TGTTCCAGAC CAAACGCTGC 
TTCCGACTGG CCCCCACCTT CAGCAACCTG CTCCTCCAGC CCACCACTGA AGCACCCGTC 
TCGGCCACCC TCAGGCGCTG TGTCAATGGA GATGTGGACA AGCCTTCAGA GCCAGCCTCT 
GAAGAGGGCT CGGAGTCGGA GGGAAGCGAG TCCAGTGGGC GCTCCTTTCG GAACGAGCGG 
AGCATCCAGG AGAAGCTGCA GGTCTTGATG GCCGAAGGCC TGCTCCCTGC TGTGAAAGTC 
TTCCTGGACT GGCTGCGGAC CAACCCCGAC CTCATCGTTG TGTGTGCGCA GAGCTCTCAA 
AGTCTGTGGA ACCGCCTGTC TGTGTTGTTG AACCTGTTGC CAGCTGCTGG TGAACTCCAA 
GAATCTGGCC TGGCCCTGTG TGCTGAGGTC CAGGAGCTGC TTGAAGGCTG TGAACTGCCT 
GACCTCCCAT CTAGCCTGCT GCTCCCAGAG GACACGGCCC TTCGCAACCT GCCTCCCCTC 
CGGGCGGCCC ACAGACGCTT TAGCTTTGAT ACAGCTCGGC TCTTGCTCAG CACCTTAGAG 
GAGACAGTCG TGCGGATCTG CTGCATACGC AGCTTTGGCC ACTTCGTTGC CCGCCTGCAA 
GGCAGCATCC TGCAGTTCAA CGCAGAGGTC GGCATCTTTG TCAGCATCGC CCAGTCTGAG 
CAGGAGAGCC TGCTGCAGCA GGCCCAGGCC CAGTTCCGCA TGGCACAGGA GGAAGCTCGG 
CGGAACAGGC TGATGAGAGA CATGGCCCAG CTACGACTTC AGCTTGAGGT CTCTCAGCTG 
GAAGGGAGCC TGCAGCAGCC CAAGGCACAG TCAGCAATGT CTCCCTACCT CGTCCCCGAC 
ACCCAGGCCC TCTGCCACCA CCTCCCTGTC ATCCGCCAGC TGGCCACCAG TGGCCGCTTC 
ATCGTCATCA TCCCAAGGAC AGTGATCGAT GGCCTGGATT TGCTGAAGAA GGAACACCCA 
GGGGCCCGGG ATGGGATCCG ATACCTGGAG GCAGAGTTTA AAAAAGGGAA CAGGTACATT 
CGCTGCCAGA GAGAGGCGGG GAAGAGCTTT GAGCGGCATA AGCTGAAGAG GCAGGATGCC 
GATGCCTGGA CGCTCTATAA GATCCTGGAC AGCTGCAAAC AGCTGACTCT GGCCCAGGGA 
GCAGGTGAGG AGGACCCGAG CGGCATGGTG ACCATCATCA CAGGCCTTCC ACTGGACAAT 
CCTAGCACGC TCTCAGGCCC TATGCAGGCA GCCTTGCAGG CCGCTGCACA TGCCAGTGTG 
GACATCAAGA ATGTTCTGGA CTTCTACAAG CAGTGGAAGG AGATTGGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

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SMG5 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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