PLEKHG4 cDNA ORF clone, Canis lupus familiaris(dog)

The following PLEKHG4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PLEKHG4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe36773 XM_014114095.2
Latest version!
Canis lupus familiaris pleckstrin homology and RhoGEF domain containing G4 (PLEKHG4), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCe36774 XM_014114096.2
Latest version!
Canis lupus familiaris pleckstrin homology and RhoGEF domain containing G4 (PLEKHG4), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCe07253 XM_005620823.3
Latest version!
Canis lupus familiaris pleckstrin homology and RhoGEF domain containing G4 (PLEKHG4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OCe07253 XM_014114094.2
Latest version!
Canis lupus familiaris pleckstrin homology and RhoGEF domain containing G4 (PLEKHG4), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OCe07253 XM_022419321.1
Latest version!
Canis lupus familiaris pleckstrin homology and RhoGEF domain containing G4 (PLEKHG4), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
View Old Accession Versions >>
OCe36773 XM_014114095.1 Canis lupus familiaris pleckstrin homology domain containing, family G (with RhoGef domain) member 4 (PLEKHG4), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCe36774 XM_014114096.1 Canis lupus familiaris pleckstrin homology domain containing, family G (with RhoGef domain) member 4 (PLEKHG4), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCe07253 XM_014114094.1 Canis lupus familiaris pleckstrin homology domain containing, family G (with RhoGef domain) member 4 (PLEKHG4), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OCe07253 XM_005620823.2 Canis lupus familiaris pleckstrin homology domain containing, family G (with RhoGef domain) member 4 (PLEKHG4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe36773
    Clone ID Related Accession (Same CDS sequence) XM_014114095.2 , XM_014114095.1
    Accession Version XM_014114095.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3618bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product puratrophin-1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014114095.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGAAGGGC CGCTGGAGGT TGGGGATGAG TCCCCAGACT CCCAGGGACA CGCCACTGAC 
    TGGAGGTTTG CTGTGTGCAG CTTCAGGGAT GCCTGGGATG AAGAGGAATC TGTTGCCCTC
    AAGCAGATTA AGGACTCCAA TCCTCCGGGT CCACCAGCAG GGACAGCCCA GGGGAGCCCC
    CTGCCTGAGG AACTTCAGTC ACCCCCAGGA CAGTTGGCTG TAGACGAGTC GGGAGATGGC
    CAGGGTTGCA CGTTAGAAGG TTCCTCACTC CGGTTAGATG AACCAATCCC CTCTGGAGTG
    GAGAGCCTCC TGTGCCCAAT GTCCTCCTGC CTCAGTCTTG AGCAGGGTGA GAGTGATAGT
    CAAGGGGGAG GCTTGGCAGG GGACCAACTT TCAAGCAGAT TAATGCCAGC CCACCTGGGT
    CCTGGGGGTT TGACCAGTGA TCCGGTGGAC CTTGGAGGCC CATCATCTGA GGTATCATCA
    GAGCTACTGG AGGCAGACCA CAGTGGCTCT AGCCTCCCTA AGCCTGCTGA TTACCTCCGG
    GCCCAAGACC TTGCCTGGGA TCTGCTGGCC AGTGGCATGG CTACCTTGCC AGGGACTCGC
    GATGTAGAAG GCCGGGCAGT GCTGCTTCTG TGTGCCCATA GCCCGGCCTG GCTTCACCTC
    AAGTGCACTA GCCATGAACT CCTCTGCCTC CTGCTCTACC TACGAGGCAT CCCCAGGCCT
    GAAGTACAGG CCCTGGGACT GACTGTTCTG GTGGATGCCC GACTTTGTTC CCCAAGCTCT
    TCCCTCCTCT GGGCGCTCAG CCAACTGCAA GAAGCAGCCC CAGGGTCAGT GTACCAGGTG
    CTGCTACTGG GAAAGATGCC GGAGGAAGTG CCTTCTGGGC TGCAGCTGCA GCAGCTGCCT
    TCTCATCAGA GCCTGCTGAA CCACGTCTCC ACCTCCGGGT TGCCGGCTTC AATGGGAGGA
    GGCCTGCCTT ATTCCCACCA GGCCTGGCTG GACTTCCGGA TGCGTCTGGA AGCCCTACTG
    CAAAGCTGCC AGGTGGCTTG TGCCCTGCTC CAGGGGGCCA TCGAGAGCAT GAAGGCTGTG
    CCCCAGCCTG TAGAGTCTGG GGAAGTTGGT CGGCTGCTAC AGCAGGCACG GGTCCTGATG
    GAGCACGTGC TAGACTCACC ACGGCTAGCA TGGCTACAAT GCCAAGGGGG TTTGGAGCTG
    GCATGGCTGA AGCAGGAAGT CCCAGAGGTG ACCCTGAGCC CAGACTACAG GTCAGCAGTG
    GATGAGGTTG ATGAACTCTA TGGCCGTGTA GATGGACTGC TGCACCAGTT GACCCTCCAG
    AGTAACCGGC GAGTACGGGC ACTAGAGTTG GTCCAGACTC TGGAGGCCCA GGAGGGTGGA
    CTACACCAGA TTGAAGTGTG GCTGCAGCAG GTGGGCTGGC CAGCCCTCGA GGAGCTAAAG
    GAGCCCTCAC TGGACATGCT GCTCCAGGCG AAAGGCCCTT TTCAGGAGCT GGACCAGGTT
    GCCCAGGAGC AGGTCAGGCG AGGGGAGAAG TTTCTACAGC CACTGGCTGG CTGGGAGGCT
    GCTGAACTGG GGCCCGCTGG GGCCCGCTTT CTGGCCCGGC AAGCCAAGCT GACTGACTTC
    TCCAGGGCTT TGACCCGGCG GCGGCAGCAG CTGGCAGATG CAGAGAGGCT ATTCCATCTT
    TTCAAGCAGG CCTCGACATG GGCTGAGGAG GGGCAGCGAG TGTTGGCAGA GCTGGAACAG
    GAGCACCCAG GGGTTGTGCT GCAACGGCTG CAGCTGCATT GGACCAAGCA CCCTGATTTG
    CCTCCTGCCC ACTTCCGAAA GATGTGGGCT CTGGCCACAG GGTTAGGTTC TGAGGGCATC
    CGCCAGGAGT GCCGCTGGGC CTGGGCACAA TGCCAGGACA CCTGGCTGGC ACTGGACCAG
    AAGCTAGAGG CTGTACTGAA GCCACCACTG ACGACGACCA TGACAGTCAG CACGGCTAGC
    CTGTGTGTCA GCAGAGTCCC AGCTGCATCT GCCACCCCTC CCCTGAGGAA GGCATACAGC
    CTGGATCAGA ATTTAGGGCA GAGTCTCAGA CAGCCTGCCC ACCACTGCCA CCACGTGGCC
    ACTGCGGCTG CCTGCCACGG ACCGGAGGCT GGAGGTGGTG CCCAGCCAGG GTCATCCCCT
    ACCATGCCTC TGCCAAGCAC CTCCGACCCC AGGAGCCCCA GCAGGCTCCA GCTGGTGCTG
    GCGGAGATGG TGGCTACGGA GCAGGAGTAT GTCCGTGCTC TTGACTACAC CATAGAGAAC
    TACTTCCCTG AGTTGGATCG TCCTGATGTG CCCCAGGGCC TCCGTGGGCA GCGTGCCCAC
    CTCTTTGGCA ACCTGGAGAA GCTGCGGGAC TTCCACTGTC ATTTCTTCCT GCGTGAACTG
    GAGGCCTGTA CCCAGCATCC ATCCCGGAGG GTGCAGTTTG GGATGTATGC ACTCTACAGC
    AAGAATAAAC CTCGCTCTGA TGCCCTGATG ACCAGCTTCG GTCATGCCTT CTTCAAGGAC
    AAGCAGCAAG CACTGGGGGA CCACCTGGAC TTGGCCTCCT ATCTGCTGAA GCCCATCCAG
    CGCATGAGCA AGTATGCATT GCTGCTGCAG GAACTGGCAC GGGCCTGTGG GGGTGCTGTG
    CAGGAGCTGA GTGCCCTGCG GGCTGCCCAG AGCCTTGTGC GCTTCCAGCT GCGACACGGC
    AATGACCTGC TAGCCATGGA TGCCATTCAG GGCTGTGATG TTAACCTCAA GGAACAGGGG
    CAGCTGGTGC GACAGGATGA GTTCATGGTA CGCACTGGGC GCCGTAAGTC TTTGCGCCGC
    GTTTTTCTTT TTGAGGAGCT GCTGCTCTTC AGCAAGCCCC GCCGAGGACC CACAGGCACC
    GACACATTTA CCTATAAGCG TTCCTTCAAG ATGGCAGACC TGGGCCTCAC TGAGTGCTGT
    GGGGATAGCA ACCTGCGGTT TGAGATCTGG TTCCGCCGTC GCAAGGCTAG GGACACCTTT
    GTGCTTCAGG CTGCCAGCTT GGCCACTAAG CAGGCTTGGA CGGCTGACAT TTCTCGTCTG
    CTCTGGAGGC AGGCCATCCA CAACAAGGAG GTGCGCATGG CTGAGATGGT GTCCATGGGT
    GTGGGGAACA AGGCCTTCCG GGACCTCGCC CCCAGTGAGG AAGCTATCAA CGACCGCACT
    GTCGACTATG TCCTGAAGTG CCGAGAAGTT CGCTCTCGGG CCTCTATTGC TGTGGCCCCA
    TTTGACTGTG ACAGCCGCTA CCTGGGGGCC TGGAGTTCCC TTCCTGGAGA CCCCGCCTCT
    TGCTCTGTAC TGGGGTCCCT CAACCTGCAC CTGTACAGAG ACCCGGCTCT TCTGGGAGTC
    CGCTGGCCCC TGTATTCTAC CAGCGTCCCA GAGGAAGCAG CGCTGGAGGC CGAGGTGGAG
    CTGGGCAGTC AGCTGTCTTT GACTCCTGAG GACTCAGAGG TCTCATCCCA GTGCCCATCA
    GTCAGTGGAT CCAGTGGCTC TGACAGCAGC TGTGTGTCAG GGCAGGTCCT GGGCAGGGGA
    CTTGAGGACT TGTCCTGTAT TCAAATGCTG AAATTCTGCC CCAGTGGAAG AAACAATAAT
    GCCCTTGAAC AACTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013969570.1
    CDS404..4021
    Translation

    Target ORF information:

    RefSeq Version XM_014114095.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris pleckstrin homology and RhoGEF domain containing G4 (PLEKHG4), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014114095.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGAAGGGC CGCTGGAGGT TGGGGATGAG TCCCCAGACT CCCAGGGACA CGCCACTGAC 
    TGGAGGTTTG CTGTGTGCAG CTTCAGGGAT GCCTGGGATG AAGAGGAATC TGTTGCCCTC
    AAGCAGATTA AGGACTCCAA TCCTCCGGGT CCACCAGCAG GGACAGCCCA GGGGAGCCCC
    CTGCCTGAGG AACTTCAGTC ACCCCCAGGA CAGTTGGCTG TAGACGAGTC GGGAGATGGC
    CAGGGTTGCA CGTTAGAAGG TTCCTCACTC CGGTTAGATG AACCAATCCC CTCTGGAGTG
    GAGAGCCTCC TGTGCCCAAT GTCCTCCTGC CTCAGTCTTG AGCAGGGTGA GAGTGATAGT
    CAAGGGGGAG GCTTGGCAGG GGACCAACTT TCAAGCAGAT TAATGCCAGC CCACCTGGGT
    CCTGGGGGTT TGACCAGTGA TCCGGTGGAC CTTGGAGGCC CATCATCTGA GGTATCATCA
    GAGCTACTGG AGGCAGACCA CAGTGGCTCT AGCCTCCCTA AGCCTGCTGA TTACCTCCGG
    GCCCAAGACC TTGCCTGGGA TCTGCTGGCC AGTGGCATGG CTACCTTGCC AGGGACTCGC
    GATGTAGAAG GCCGGGCAGT GCTGCTTCTG TGTGCCCATA GCCCGGCCTG GCTTCACCTC
    AAGTGCACTA GCCATGAACT CCTCTGCCTC CTGCTCTACC TACGAGGCAT CCCCAGGCCT
    GAAGTACAGG CCCTGGGACT GACTGTTCTG GTGGATGCCC GACTTTGTTC CCCAAGCTCT
    TCCCTCCTCT GGGCGCTCAG CCAACTGCAA GAAGCAGCCC CAGGGTCAGT GTACCAGGTG
    CTGCTACTGG GAAAGATGCC GGAGGAAGTG CCTTCTGGGC TGCAGCTGCA GCAGCTGCCT
    TCTCATCAGA GCCTGCTGAA CCACGTCTCC ACCTCCGGGT TGCCGGCTTC AATGGGAGGA
    GGCCTGCCTT ATTCCCACCA GGCCTGGCTG GACTTCCGGA TGCGTCTGGA AGCCCTACTG
    CAAAGCTGCC AGGTGGCTTG TGCCCTGCTC CAGGGGGCCA TCGAGAGCAT GAAGGCTGTG
    CCCCAGCCTG TAGAGTCTGG GGAAGTTGGT CGGCTGCTAC AGCAGGCACG GGTCCTGATG
    GAGCACGTGC TAGACTCACC ACGGCTAGCA TGGCTACAAT GCCAAGGGGG TTTGGAGCTG
    GCATGGCTGA AGCAGGAAGT CCCAGAGGTG ACCCTGAGCC CAGACTACAG GTCAGCAGTG
    GATGAGGTTG ATGAACTCTA TGGCCGTGTA GATGGACTGC TGCACCAGTT GACCCTCCAG
    AGTAACCGGC GAGTACGGGC ACTAGAGTTG GTCCAGACTC TGGAGGCCCA GGAGGGTGGA
    CTACACCAGA TTGAAGTGTG GCTGCAGCAG GTGGGCTGGC CAGCCCTCGA GGAGCTAAAG
    GAGCCCTCAC TGGACATGCT GCTCCAGGCG AAAGGCCCTT TTCAGGAGCT GGACCAGGTT
    GCCCAGGAGC AGGTCAGGCG AGGGGAGAAG TTTCTACAGC CACTGGCTGG CTGGGAGGCT
    GCTGAACTGG GGCCCGCTGG GGCCCGCTTT CTGGCCCGGC AAGCCAAGCT GACTGACTTC
    TCCAGGGCTT TGACCCGGCG GCGGCAGCAG CTGGCAGATG CAGAGAGGCT ATTCCATCTT
    TTCAAGCAGG CCTCGACATG GGCTGAGGAG GGGCAGCGAG TGTTGGCAGA GCTGGAACAG
    GAGCACCCAG GGGTTGTGCT GCAACGGCTG CAGCTGCATT GGACCAAGCA CCCTGATTTG
    CCTCCTGCCC ACTTCCGAAA GATGTGGGCT CTGGCCACAG GGTTAGGTTC TGAGGGCATC
    CGCCAGGAGT GCCGCTGGGC CTGGGCACAA TGCCAGGACA CCTGGCTGGC ACTGGACCAG
    AAGCTAGAGG CTGTACTGAA GCCACCACTG ACGACGACCA TGACAGTCAG CACGGCTAGC
    CTGTGTGTCA GCAGAGTCCC AGCTGCATCT GCCACCCCTC CCCTGAGGAA GGCATACAGC
    CTGGATCAGA ATTTAGGGCA GAGTCTCAGA CAGCCTGCCC ACCACTGCCA CCACGTGGCC
    ACTGCGGCTG CCTGCCACGG ACCGGAGGCT GGAGGTGGTG CCCAGCCAGG GTCATCCCCT
    ACCATGCCTC TGCCAAGCAC CTCCGACCCC AGGAGCCCCA GCAGGCTCCA GCTGGTGCTG
    GCGGAGATGG TGGCTACGGA GCAGGAGTAT GTCCGTGCTC TTGACTACAC CATAGAGAAC
    TACTTCCCTG AGTTGGATCG TCCTGATGTG CCCCAGGGCC TCCGTGGGCA GCGTGCCCAC
    CTCTTTGGCA ACCTGGAGAA GCTGCGGGAC TTCCACTGTC ATTTCTTCCT GCGTGAACTG
    GAGGCCTGTA CCCAGCATCC ATCCCGGAGG GTGCAGTTTG GGATGTATGC ACTCTACAGC
    AAGAATAAAC CTCGCTCTGA TGCCCTGATG ACCAGCTTCG GTCATGCCTT CTTCAAGGAC
    AAGCAGCAAG CACTGGGGGA CCACCTGGAC TTGGCCTCCT ATCTGCTGAA GCCCATCCAG
    CGCATGAGCA AGTATGCATT GCTGCTGCAG GAACTGGCAC GGGCCTGTGG GGGTGCTGTG
    CAGGAGCTGA GTGCCCTGCG GGCTGCCCAG AGCCTTGTGC GCTTCCAGCT GCGACACGGC
    AATGACCTGC TAGCCATGGA TGCCATTCAG GGCTGTGATG TTAACCTCAA GGAACAGGGG
    CAGCTGGTGC GACAGGATGA GTTCATGGTA CGCACTGGGC GCCGTAAGTC TTTGCGCCGC
    GTTTTTCTTT TTGAGGAGCT GCTGCTCTTC AGCAAGCCCC GCCGAGGACC CACAGGCACC
    GACACATTTA CCTATAAGCG TTCCTTCAAG ATGGCAGACC TGGGCCTCAC TGAGTGCTGT
    GGGGATAGCA ACCTGCGGTT TGAGATCTGG TTCCGCCGTC GCAAGGCTAG GGACACCTTT
    GTGCTTCAGG CTGCCAGCTT GGCCACTAAG CAGGCTTGGA CGGCTGACAT TTCTCGTCTG
    CTCTGGAGGC AGGCCATCCA CAACAAGGAG GTGCGCATGG CTGAGATGGT GTCCATGGGT
    GTGGGGAACA AGGCCTTCCG GGACCTCGCC CCCAGTGAGG AAGCTATCAA CGACCGCACT
    GTCGACTATG TCCTGAAGTG CCGAGAAGTT CGCTCTCGGG CCTCTATTGC TGTGGCCCCA
    TTTGACTGTG ACAGCCGCTA CCTGGGGGCC TGGAGTTCCC TTCCTGGAGA CCCCGCCTCT
    TGCTCTGTAC TGGGGTCCCT CAACCTGCAC CTGTACAGAG ACCCGGCTCT TCTGGGAGTC
    CGCTGGCCCC TGTATTCTAC CAGCGTCCCA GAGGAAGCAG CGCTGGAGGC CGAGGTGGAG
    CTGGGCAGTC AGCTGTCTTT GACTCCTGAG GACTCAGAGG TCTCATCCCA GTGCCCATCA
    GTCAGTGGAT CCAGTGGCTC TGACAGCAGC TGTGTGTCAG GGCAGGTCCT GGGCAGGGGA
    CTTGAGGACT TGTCCTGTAT TCAAATGCTG AAATTCTGCC CCAGTGGAAG AAACAATAAT
    GCCCTTGAAC AACTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe36773
    Clone ID Related Accession (Same CDS sequence) XM_014114095.2 , XM_014114095.1
    Accession Version XM_014114095.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3618bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product puratrophin-1 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726060.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGAAGGGC CGCTGGAGGT TGGGGATGAG TCCCCAGACT CCCAGGGACA CGCCACTGAC 
    TGGAGGTTTG CTGTGTGCAG CTTCAGGGAT GCCTGGGATG AAGAGGAATC TGTTGCCCTC
    AAGCAGATTA AGGACTCCAA TCCTCCGGGT CCACCAGCAG GGACAGCCCA GGGGAGCCCC
    CTGCCTGAGG AACTTCAGTC ACCCCCAGGA CAGTTGGCTG TAGACGAGTC GGGAGATGGC
    CAGGGTTGCA CGTTAGAAGG TTCCTCACTC CGGTTAGATG AACCAATCCC CTCTGGAGTG
    GAGAGCCTCC TGTGCCCAAT GTCCTCCTGC CTCAGTCTTG AGCAGGGTGA GAGTGATAGT
    CAAGGGGGAG GCTTGGCAGG GGACCAACTT TCAAGCAGAT TAATGCCAGC CCACCTGGGT
    CCTGGGGGTT TGACCAGTGA TCCGGTGGAC CTTGGAGGCC CATCATCTGA GGTATCATCA
    GAGCTACTGG AGGCAGACCA CAGTGGCTCT AGCCTCCCTA AGCCTGCTGA TTACCTCCGG
    GCCCAAGACC TTGCCTGGGA TCTGCTGGCC AGTGGCATGG CTACCTTGCC AGGGACTCGC
    GATGTAGAAG GCCGGGCAGT GCTGCTTCTG TGTGCCCATA GCCCGGCCTG GCTTCACCTC
    AAGTGCACTA GCCATGAACT CCTCTGCCTC CTGCTCTACC TACGAGGCAT CCCCAGGCCT
    GAAGTACAGG CCCTGGGACT GACTGTTCTG GTGGATGCCC GACTTTGTTC CCCAAGCTCT
    TCCCTCCTCT GGGCGCTCAG CCAACTGCAA GAAGCAGCCC CAGGGTCAGT GTACCAGGTG
    CTGCTACTGG GAAAGATGCC GGAGGAAGTG CCTTCTGGGC TGCAGCTGCA GCAGCTGCCT
    TCTCATCAGA GCCTGCTGAA CCACGTCTCC ACCTCCGGGT TGCCGGCTTC AATGGGAGGA
    GGCCTGCCTT ATTCCCACCA GGCCTGGCTG GACTTCCGGA TGCGTCTGGA AGCCCTACTG
    CAAAGCTGCC AGGTGGCTTG TGCCCTGCTC CAGGGGGCCA TCGAGAGCAT GAAGGCTGTG
    CCCCAGCCTG TAGAGTCTGG GGAAGTTGGT CGGCTGCTAC AGCAGGCACG GGTCCTGATG
    GAGCACGTGC TAGACTCACC ACGGCTAGCA TGGCTACAAT GCCAAGGGGG TTTGGAGCTG
    GCATGGCTGA AGCAGGAAGT CCCAGAGGTG ACCCTGAGCC CAGACTACAG GTCAGCAGTG
    GATGAGGTTG ATGAACTCTA TGGCCGTGTA GATGGACTGC TGCACCAGTT GACCCTCCAG
    AGTAACCGGC GAGTACGGGC ACTAGAGTTG GTCCAGACTC TGGAGGCCCA GGAGGGTGGA
    CTACACCAGA TTGAAGTGTG GCTGCAGCAG GTGGGCTGGC CAGCCCTCGA GGAGCTAAAG
    GAGCCCTCAC TGGACATGCT GCTCCAGGCG AAAGGCCCTT TTCAGGAGCT GGACCAGGTT
    GCCCAGGAGC AGGTCAGGCG AGGGGAGAAG TTTCTACAGC CACTGGCTGG CTGGGAGGCT
    GCTGAACTGG GGCCCGCTGG GGCCCGCTTT CTGGCCCGGC AAGCCAAGCT GACTGACTTC
    TCCAGGGCTT TGACCCGGCG GCGGCAGCAG CTGGCAGATG CAGAGAGGCT ATTCCATCTT
    TTCAAGCAGG CCTCGACATG GGCTGAGGAG GGGCAGCGAG TGTTGGCAGA GCTGGAACAG
    GAGCACCCAG GGGTTGTGCT GCAACGGCTG CAGCTGCATT GGACCAAGCA CCCTGATTTG
    CCTCCTGCCC ACTTCCGAAA GATGTGGGCT CTGGCCACAG GGTTAGGTTC TGAGGGCATC
    CGCCAGGAGT GCCGCTGGGC CTGGGCACAA TGCCAGGACA CCTGGCTGGC ACTGGACCAG
    AAGCTAGAGG CTGTACTGAA GCCACCACTG ACGACGACCA TGACAGTCAG CACGGCTAGC
    CTGTGTGTCA GCAGAGTCCC AGCTGCATCT GCCACCCCTC CCCTGAGGAA GGCATACAGC
    CTGGATCAGA ATTTAGGGCA GAGTCTCAGA CAGCCTGCCC ACCACTGCCA CCACGTGGCC
    ACTGCGGCTG CCTGCCACGG ACCGGAGGCT GGAGGTGGTG CCCAGCCAGG GTCATCCCCT
    ACCATGCCTC TGCCAAGCAC CTCCGACCCC AGGAGCCCCA GCAGGCTCCA GCTGGTGCTG
    GCGGAGATGG TGGCTACGGA GCAGGAGTAT GTCCGTGCTC TTGACTACAC CATAGAGAAC
    TACTTCCCTG AGTTGGATCG TCCTGATGTG CCCCAGGGCC TCCGTGGGCA GCGTGCCCAC
    CTCTTTGGCA ACCTGGAGAA GCTGCGGGAC TTCCACTGTC ATTTCTTCCT GCGTGAACTG
    GAGGCCTGTA CCCAGCATCC ATCCCGGAGG GTGCAGTTTG GGATGTATGC ACTCTACAGC
    AAGAATAAAC CTCGCTCTGA TGCCCTGATG ACCAGCTTCG GTCATGCCTT CTTCAAGGAC
    AAGCAGCAAG CACTGGGGGA CCACCTGGAC TTGGCCTCCT ATCTGCTGAA GCCCATCCAG
    CGCATGAGCA AGTATGCATT GCTGCTGCAG GAACTGGCAC GGGCCTGTGG GGGTGCTGTG
    CAGGAGCTGA GTGCCCTGCG GGCTGCCCAG AGCCTTGTGC GCTTCCAGCT GCGACACGGC
    AATGACCTGC TAGCCATGGA TGCCATTCAG GGCTGTGATG TTAACCTCAA GGAACAGGGG
    CAGCTGGTGC GACAGGATGA GTTCATGGTA CGCACTGGGC GCCGTAAGTC TTTGCGCCGC
    GTTTTTCTTT TTGAGGAGCT GCTGCTCTTC AGCAAGCCCC GCCGAGGACC CACAGGCACC
    GACACATTTA CCTATAAGCG TTCCTTCAAG ATGGCAGACC TGGGCCTCAC TGAGTGCTGT
    GGGGATAGCA ACCTGCGGTT TGAGATCTGG TTCCGCCGTC GCAAGGCTAG GGACACCTTT
    GTGCTTCAGG CTGCCAGCTT GGCCACTAAG CAGGCTTGGA CGGCTGACAT TTCTCGTCTG
    CTCTGGAGGC AGGCCATCCA CAACAAGGAG GTGCGCATGG CTGAGATGGT GTCCATGGGT
    GTGGGGAACA AGGCCTTCCG GGACCTCGCC CCCAGTGAGG AAGCTATCAA CGACCGCACT
    GTCGACTATG TCCTGAAGTG CCGAGAAGTT CGCTCTCGGG CCTCTATTGC TGTGGCCCCA
    TTTGACTGTG ACAGCCGCTA CCTGGGGGCC TGGAGTTCCC TTCCTGGAGA CCCCGCCTCT
    TGCTCTGTAC TGGGGTCCCT CAACCTGCAC CTGTACAGAG ACCCGGCTCT TCTGGGAGTC
    CGCTGGCCCC TGTATTCTAC CAGCGTCCCA GAGGAAGCAG CGCTGGAGGC CGAGGTGGAG
    CTGGGCAGTC AGCTGTCTTT GACTCCTGAG GACTCAGAGG TCTCATCCCA GTGCCCATCA
    GTCAGTGGAT CCAGTGGCTC TGACAGCAGC TGTGTGTCAG GGCAGGTCCT GGGCAGGGGA
    CTTGAGGACT TGTCCTGTAT TCAAATGCTG AAATTCTGCC CCAGTGGAAG AAACAATAAT
    GCCCTTGAAC AACTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013969570.1
    CDS467..4084
    Translation

    Target ORF information:

    RefSeq Version XM_014114095.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris pleckstrin homology domain containing, family G (with RhoGef domain) member 4 (PLEKHG4), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014114095.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGAAGGGC CGCTGGAGGT TGGGGATGAG TCCCCAGACT CCCAGGGACA CGCCACTGAC 
    TGGAGGTTTG CTGTGTGCAG CTTCAGGGAT GCCTGGGATG AAGAGGAATC TGTTGCCCTC
    AAGCAGATTA AGGACTCCAA TCCTCCGGGT CCACCAGCAG GGACAGCCCA GGGGAGCCCC
    CTGCCTGAGG AACTTCAGTC ACCCCCAGGA CAGTTGGCTG TAGACGAGTC GGGAGATGGC
    CAGGGTTGCA CGTTAGAAGG TTCCTCACTC CGGTTAGATG AACCAATCCC CTCTGGAGTG
    GAGAGCCTCC TGTGCCCAAT GTCCTCCTGC CTCAGTCTTG AGCAGGGTGA GAGTGATAGT
    CAAGGGGGAG GCTTGGCAGG GGACCAACTT TCAAGCAGAT TAATGCCAGC CCACCTGGGT
    CCTGGGGGTT TGACCAGTGA TCCGGTGGAC CTTGGAGGCC CATCATCTGA GGTATCATCA
    GAGCTACTGG AGGCAGACCA CAGTGGCTCT AGCCTCCCTA AGCCTGCTGA TTACCTCCGG
    GCCCAAGACC TTGCCTGGGA TCTGCTGGCC AGTGGCATGG CTACCTTGCC AGGGACTCGC
    GATGTAGAAG GCCGGGCAGT GCTGCTTCTG TGTGCCCATA GCCCGGCCTG GCTTCACCTC
    AAGTGCACTA GCCATGAACT CCTCTGCCTC CTGCTCTACC TACGAGGCAT CCCCAGGCCT
    GAAGTACAGG CCCTGGGACT GACTGTTCTG GTGGATGCCC GACTTTGTTC CCCAAGCTCT
    TCCCTCCTCT GGGCGCTCAG CCAACTGCAA GAAGCAGCCC CAGGGTCAGT GTACCAGGTG
    CTGCTACTGG GAAAGATGCC GGAGGAAGTG CCTTCTGGGC TGCAGCTGCA GCAGCTGCCT
    TCTCATCAGA GCCTGCTGAA CCACGTCTCC ACCTCCGGGT TGCCGGCTTC AATGGGAGGA
    GGCCTGCCTT ATTCCCACCA GGCCTGGCTG GACTTCCGGA TGCGTCTGGA AGCCCTACTG
    CAAAGCTGCC AGGTGGCTTG TGCCCTGCTC CAGGGGGCCA TCGAGAGCAT GAAGGCTGTG
    CCCCAGCCTG TAGAGTCTGG GGAAGTTGGT CGGCTGCTAC AGCAGGCACG GGTCCTGATG
    GAGCACGTGC TAGACTCACC ACGGCTAGCA TGGCTACAAT GCCAAGGGGG TTTGGAGCTG
    GCATGGCTGA AGCAGGAAGT CCCAGAGGTG ACCCTGAGCC CAGACTACAG GTCAGCAGTG
    GATGAGGTTG ATGAACTCTA TGGCCGTGTA GATGGACTGC TGCACCAGTT GACCCTCCAG
    AGTAACCGGC GAGTACGGGC ACTAGAGTTG GTCCAGACTC TGGAGGCCCA GGAGGGTGGA
    CTACACCAGA TTGAAGTGTG GCTGCAGCAG GTGGGCTGGC CAGCCCTCGA GGAGCTAAAG
    GAGCCCTCAC TGGACATGCT GCTCCAGGCG AAAGGCCCTT TTCAGGAGCT GGACCAGGTT
    GCCCAGGAGC AGGTCAGGCG AGGGGAGAAG TTTCTACAGC CACTGGCTGG CTGGGAGGCT
    GCTGAACTGG GGCCCGCTGG GGCCCGCTTT CTGGCCCGGC AAGCCAAGCT GACTGACTTC
    TCCAGGGCTT TGACCCGGCG GCGGCAGCAG CTGGCAGATG CAGAGAGGCT ATTCCATCTT
    TTCAAGCAGG CCTCGACATG GGCTGAGGAG GGGCAGCGAG TGTTGGCAGA GCTGGAACAG
    GAGCACCCAG GGGTTGTGCT GCAACGGCTG CAGCTGCATT GGACCAAGCA CCCTGATTTG
    CCTCCTGCCC ACTTCCGAAA GATGTGGGCT CTGGCCACAG GGTTAGGTTC TGAGGGCATC
    CGCCAGGAGT GCCGCTGGGC CTGGGCACAA TGCCAGGACA CCTGGCTGGC ACTGGACCAG
    AAGCTAGAGG CTGTACTGAA GCCACCACTG ACGACGACCA TGACAGTCAG CACGGCTAGC
    CTGTGTGTCA GCAGAGTCCC AGCTGCATCT GCCACCCCTC CCCTGAGGAA GGCATACAGC
    CTGGATCAGA ATTTAGGGCA GAGTCTCAGA CAGCCTGCCC ACCACTGCCA CCACGTGGCC
    ACTGCGGCTG CCTGCCACGG ACCGGAGGCT GGAGGTGGTG CCCAGCCAGG GTCATCCCCT
    ACCATGCCTC TGCCAAGCAC CTCCGACCCC AGGAGCCCCA GCAGGCTCCA GCTGGTGCTG
    GCGGAGATGG TGGCTACGGA GCAGGAGTAT GTCCGTGCTC TTGACTACAC CATAGAGAAC
    TACTTCCCTG AGTTGGATCG TCCTGATGTG CCCCAGGGCC TCCGTGGGCA GCGTGCCCAC
    CTCTTTGGCA ACCTGGAGAA GCTGCGGGAC TTCCACTGTC ATTTCTTCCT GCGTGAACTG
    GAGGCCTGTA CCCAGCATCC ATCCCGGAGG GTGCAGTTTG GGATGTATGC ACTCTACAGC
    AAGAATAAAC CTCGCTCTGA TGCCCTGATG ACCAGCTTCG GTCATGCCTT CTTCAAGGAC
    AAGCAGCAAG CACTGGGGGA CCACCTGGAC TTGGCCTCCT ATCTGCTGAA GCCCATCCAG
    CGCATGAGCA AGTATGCATT GCTGCTGCAG GAACTGGCAC GGGCCTGTGG GGGTGCTGTG
    CAGGAGCTGA GTGCCCTGCG GGCTGCCCAG AGCCTTGTGC GCTTCCAGCT GCGACACGGC
    AATGACCTGC TAGCCATGGA TGCCATTCAG GGCTGTGATG TTAACCTCAA GGAACAGGGG
    CAGCTGGTGC GACAGGATGA GTTCATGGTA CGCACTGGGC GCCGTAAGTC TTTGCGCCGC
    GTTTTTCTTT TTGAGGAGCT GCTGCTCTTC AGCAAGCCCC GCCGAGGACC CACAGGCACC
    GACACATTTA CCTATAAGCG TTCCTTCAAG ATGGCAGACC TGGGCCTCAC TGAGTGCTGT
    GGGGATAGCA ACCTGCGGTT TGAGATCTGG TTCCGCCGTC GCAAGGCTAG GGACACCTTT
    GTGCTTCAGG CTGCCAGCTT GGCCACTAAG CAGGCTTGGA CGGCTGACAT TTCTCGTCTG
    CTCTGGAGGC AGGCCATCCA CAACAAGGAG GTGCGCATGG CTGAGATGGT GTCCATGGGT
    GTGGGGAACA AGGCCTTCCG GGACCTCGCC CCCAGTGAGG AAGCTATCAA CGACCGCACT
    GTCGACTATG TCCTGAAGTG CCGAGAAGTT CGCTCTCGGG CCTCTATTGC TGTGGCCCCA
    TTTGACTGTG ACAGCCGCTA CCTGGGGGCC TGGAGTTCCC TTCCTGGAGA CCCCGCCTCT
    TGCTCTGTAC TGGGGTCCCT CAACCTGCAC CTGTACAGAG ACCCGGCTCT TCTGGGAGTC
    CGCTGGCCCC TGTATTCTAC CAGCGTCCCA GAGGAAGCAG CGCTGGAGGC CGAGGTGGAG
    CTGGGCAGTC AGCTGTCTTT GACTCCTGAG GACTCAGAGG TCTCATCCCA GTGCCCATCA
    GTCAGTGGAT CCAGTGGCTC TGACAGCAGC TGTGTGTCAG GGCAGGTCCT GGGCAGGGGA
    CTTGAGGACT TGTCCTGTAT TCAAATGCTG AAATTCTGCC CCAGTGGAAG AAACAATAAT
    GCCCTTGAAC AACTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe36774
    Clone ID Related Accession (Same CDS sequence) XM_014114096.1 , XM_014114096.2
    Accession Version XM_014114096.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3588bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product puratrophin-1 isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726060.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGAAGGGC CGCTGGAGGT TGGGGATGAG TCCCCAGACT CCCAGGGACA CGCCACTGAC 
    TGGAGGTTTG CTGTGTGCAG CTTCAGGGAT GCCTGGGATG AAGAGGAATC TGTTGCCCTC
    AAGCAGATTA AGGACTCCAA TCCTCCGGGT CCACCAGCAG GGACAGCCCA GGGGAGCCCC
    CTGCCTGAGG AACTTCAGTC ACCCCCAGGA CAGTTGGCTG TAGACGAGTC GGGAGATGGC
    CAGGGTTGCA CGTTAGAAGG TTCCTCACTC CGGTTAGATG AACCAATCCC CTCTGGAGTG
    GAGAGCCTCC TGTGCCCAAT GTCCTCCTGC CTCAGTCTTG AGCAGGGTGA GAGTGATAGT
    CAAGGGGGAG GCTTGGCAGG GGACCAACTT TCAAGCAGAT TAATGCCAGC CCACCTGGGT
    CCTGGGGGTT TGACCAGTGA TCCGGTGGAC CTTGGAGGCC CATCATCTGA GGTATCATCA
    GAGCTACTGG AGGCAGACCA CAGTGGCTCT AGCCTCCCTA AGCCTGCTGA TTACCTCCGG
    GCCCAAGACC TTGCCTGGGA TCTGCTGGCC AGTGGCATGG CTACCTTGCC AGGGACTCGC
    GATGTAGAAG GCCGGGCAGT GCTGCTTCTG TGTGCCCATA GCCCGGCCTG GCTTCACCTC
    AAGTGCACTA GCCATGAACT CCTCTGCCTC CTGCTCTACC TACGAGGCAT CCCCAGGCCT
    GAAGTACAGG CCCTGGGACT GACTGTTCTG GTGGATGCCC GACTTTGTTC CCCAAGCTCT
    TCCCTCCTCT GGGCGCTCAG CCAACTGCAA GAAGCAGCCC CAGGGTCAGT GTACCAGGTG
    CTGCTACTGG GAAAGATGCC GGAGGAAGTG CCTTCTGGGC TGCAGCTGCA GCAGCTGCCT
    TCTCATCAGA GCCTGCTGAA CCACGTCTCC ACCTCCGGGT TGCCGGCTTC AATGGGAGGA
    GGCCTGCCTT ATTCCCACCA GGCCTGGCTG GACTTCCGGA TGCGTCTGGA AGCCCTACTG
    CAAAGCTGCC AGGTGGCTTG TGCCCTGCTC CAGGGGGCCA TCGAGAGCAT GAAGGCTGTG
    CCCCAGCCTG TAGAGTCTGG GGAAGTTGGT CGGCTGCTAC AGCAGGCACG GGTCCTGATG
    GAGCACGTGC TAGACTCACC ACGGCTAGCA TGGCTACAAT GCCAAGGGGG TTTGGAGCTG
    GCATGGCTGA AGCAGGAAGT CCCAGAGGTG ACCCTGAGCC CAGACTACAG GTCAGCAGTG
    GATGAGGTTG ATGAACTCTA TGGCCGTGTA GATGGACTGC TGCACCAGTT GACCCTCCAG
    AGTAACCGGC GAGTACGGGC ACTAGAGTTG GTCCAGACTC TGGAGGCCCA GGAGGGTGGA
    CTACACCAGA TTGAAGTGTG GCTGCAGCAG GTGGGCTGGC CAGCCCTCGA GGAGCTAAAG
    GAGCCCTCAC TGGACATGCT GCTCCAGGCG AAAGGCCCTT TTCAGGAGCT GGACCAGGTT
    GCCCAGGAGC AGGTCAGGCG AGGGGAGAAG TTTCTACAGC CACTGGCTGG CTGGGAGGCT
    GCTGAACTGG GGCCCGCTGG GGCCCGCTTT CTGGCCCGGC AAGCCAAGCT GACTGACTTC
    TCCAGGGCTT TGACCCGGCG GCGGCAGCAG CTGGCAGATG CAGAGAGGCT ATTCCATCTT
    TTCAAGCAGG CCTCGACATG GGCTGAGGAG GGGCAGCGAG TGTTGGCAGA GCTGGAACAG
    GAGCACCCAG GGGTTGTGCT GCAACGGCTG CAGCTGCATT GGACCAAGCA CCCTGATTTG
    CCTCCTGCCC ACTTCCGAAA GATGTGGGCT CTGGCCACAG GGTTAGGTTC TGAGGGCATC
    CGCCAGGAGT GCCGCTGGGC CTGGGCACAA TGCCAGGACA CCTGGCTGGC ACTGGACCAG
    AAGCTAGAGG CTGTACTGAA GCCACCACTG ACGACGACCA TGACAGTCAG CACGGCTAGC
    CTGTGTGTCA GCAGAGTCCC AGCTGCATCT GCCACCCCTC CCCTGAGGAA GGCATACAGC
    CTGGATCAGA ATTTAGGGCA GAGTCTCAGA CAGCCTGCCC ACCACTGCCA CCACGTGGCC
    ACTGCGGCTG CCTGCCACGG ACCGGAGGCT GGAGGTGGTG CCCAGCCAGG GTCATCCCCT
    ACCATGCCTC TGCCAAGCAC CTCCGACCCC AGGAGCCCCA GCAGGCTCCA GCTGGTGCTG
    GCGGAGATGG TGGCTACGGA GCAGGAGTAT GTCCGTGCTC TTGACTACAC CATAGAGAAC
    TACTTCCCTG AGTTGGATCG TCCTGATGTG CCCCAGGGCC TCCGTGGGCA GCGTGCCCAC
    CTCTTTGGCA ACCTGGAGAA GCTGCGGGAC TTCCACTGTC ATTTCTTCCT GCGTGAACTG
    GAGGCCTGTA CCCAGCATCC ATCCCGGGTA GCCTATGCCT TCCTGCGCCA TAGGGTGCAG
    TTTGGGATGT ATGCACTCTA CAGCAAGAAT AAACCTCGCT CTGATGCCCT GATGACCAGC
    TTCGGTCATG CCTTCTTCAA GGACAAGCAG CAAGCACTGG GGGACCACCT GGACTTGGCC
    TCCTATCTGC TGAAGCCCAT CCAGCGCATG AGCAAGTATG CATTGCTGCT GCAGGAACTG
    GCACGGGCCT GTGGGGGTGC TGTGCAGGAG CTGAGTGCCC TGCGGGCTGC CCAGAGCCTT
    GTGCGCTTCC AGCTGCGACA CGGCAATGAC CTGCTAGCCA TGGATGCCAT TCAGGGCTGT
    GATGTTAACC TCAAGGAACA GGGGCAGCTG GTGCGACAGG ATGAGTTCAT GGTACGCACT
    GGGCGCCGTA AGTCTTTGCG CCGCGTTTTT CTTTTTGAGG AGCTGCTGCT CTTCAGCAAG
    CCCCGCCGAG GACCCACAGG CACCGACACA TTTACCTATA AGCGTTCCTT CAAGATGGCA
    GACCTGGGCC TCACTGAGTG CTGTGGGGAT AGCAACCTGC GGTTTGAGAT CTGGTTCCGC
    CGTCGCAAGG CTAGGGACAC CTTTGTGCTT CAGGCTGCCA GCTTGGCCAC TAAGCAGGCT
    TGGACGGCTG ACATTTCTCG TCTGCTCTGG AGGCAGGCCA TCCACAACAA GGAGGTGCGC
    ATGGCTGAGA TGGTGTCCAT GGGTGTGGGG AACAAGGCCT TCCGGGACCT CGCCCCCAGT
    GAGGAAGCTA TCAACGACCG CACTGTCGAC TATGTCCTGA AGTGCCGAGA AGTTCGCTCT
    CGGGCCTCTA TTGCTGTGGC CCCATTTGAC TGTGACAGCC GCTACCTGGG GGCCTGGAGT
    TCCCTTCCTG GAGACCCCGC CTCTTGCTCT GTACTGGGGT CCCTCAACCT GCACCTGTAC
    AGAGACCCGG CTCTTCTGGG AGTCCGCTGG CCCCTGTATT CTACCAGCGT CCCAGAGGAA
    GCAGCGCTGG AGGCCGAGGT GGAGCTGGGC AGTCAGCTGT CTTTGACTCC TGAGGACTCA
    GAGGTCTCAT CCCAGTGCCC ATCAGTCAGT GGATCCAGTG GCTCTGACAG CAGCTGTGTG
    TCAGGGCAGG TCCTGGGCAG GGGACTTGAG GACTTGTCCT GTGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013969571.1
    CDS467..4054
    Translation

    Target ORF information:

    RefSeq Version XM_014114096.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris pleckstrin homology domain containing, family G (with RhoGef domain) member 4 (PLEKHG4), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014114096.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGAAGGGC CGCTGGAGGT TGGGGATGAG TCCCCAGACT CCCAGGGACA CGCCACTGAC 
    TGGAGGTTTG CTGTGTGCAG CTTCAGGGAT GCCTGGGATG AAGAGGAATC TGTTGCCCTC
    AAGCAGATTA AGGACTCCAA TCCTCCGGGT CCACCAGCAG GGACAGCCCA GGGGAGCCCC
    CTGCCTGAGG AACTTCAGTC ACCCCCAGGA CAGTTGGCTG TAGACGAGTC GGGAGATGGC
    CAGGGTTGCA CGTTAGAAGG TTCCTCACTC CGGTTAGATG AACCAATCCC CTCTGGAGTG
    GAGAGCCTCC TGTGCCCAAT GTCCTCCTGC CTCAGTCTTG AGCAGGGTGA GAGTGATAGT
    CAAGGGGGAG GCTTGGCAGG GGACCAACTT TCAAGCAGAT TAATGCCAGC CCACCTGGGT
    CCTGGGGGTT TGACCAGTGA TCCGGTGGAC CTTGGAGGCC CATCATCTGA GGTATCATCA
    GAGCTACTGG AGGCAGACCA CAGTGGCTCT AGCCTCCCTA AGCCTGCTGA TTACCTCCGG
    GCCCAAGACC TTGCCTGGGA TCTGCTGGCC AGTGGCATGG CTACCTTGCC AGGGACTCGC
    GATGTAGAAG GCCGGGCAGT GCTGCTTCTG TGTGCCCATA GCCCGGCCTG GCTTCACCTC
    AAGTGCACTA GCCATGAACT CCTCTGCCTC CTGCTCTACC TACGAGGCAT CCCCAGGCCT
    GAAGTACAGG CCCTGGGACT GACTGTTCTG GTGGATGCCC GACTTTGTTC CCCAAGCTCT
    TCCCTCCTCT GGGCGCTCAG CCAACTGCAA GAAGCAGCCC CAGGGTCAGT GTACCAGGTG
    CTGCTACTGG GAAAGATGCC GGAGGAAGTG CCTTCTGGGC TGCAGCTGCA GCAGCTGCCT
    TCTCATCAGA GCCTGCTGAA CCACGTCTCC ACCTCCGGGT TGCCGGCTTC AATGGGAGGA
    GGCCTGCCTT ATTCCCACCA GGCCTGGCTG GACTTCCGGA TGCGTCTGGA AGCCCTACTG
    CAAAGCTGCC AGGTGGCTTG TGCCCTGCTC CAGGGGGCCA TCGAGAGCAT GAAGGCTGTG
    CCCCAGCCTG TAGAGTCTGG GGAAGTTGGT CGGCTGCTAC AGCAGGCACG GGTCCTGATG
    GAGCACGTGC TAGACTCACC ACGGCTAGCA TGGCTACAAT GCCAAGGGGG TTTGGAGCTG
    GCATGGCTGA AGCAGGAAGT CCCAGAGGTG ACCCTGAGCC CAGACTACAG GTCAGCAGTG
    GATGAGGTTG ATGAACTCTA TGGCCGTGTA GATGGACTGC TGCACCAGTT GACCCTCCAG
    AGTAACCGGC GAGTACGGGC ACTAGAGTTG GTCCAGACTC TGGAGGCCCA GGAGGGTGGA
    CTACACCAGA TTGAAGTGTG GCTGCAGCAG GTGGGCTGGC CAGCCCTCGA GGAGCTAAAG
    GAGCCCTCAC TGGACATGCT GCTCCAGGCG AAAGGCCCTT TTCAGGAGCT GGACCAGGTT
    GCCCAGGAGC AGGTCAGGCG AGGGGAGAAG TTTCTACAGC CACTGGCTGG CTGGGAGGCT
    GCTGAACTGG GGCCCGCTGG GGCCCGCTTT CTGGCCCGGC AAGCCAAGCT GACTGACTTC
    TCCAGGGCTT TGACCCGGCG GCGGCAGCAG CTGGCAGATG CAGAGAGGCT ATTCCATCTT
    TTCAAGCAGG CCTCGACATG GGCTGAGGAG GGGCAGCGAG TGTTGGCAGA GCTGGAACAG
    GAGCACCCAG GGGTTGTGCT GCAACGGCTG CAGCTGCATT GGACCAAGCA CCCTGATTTG
    CCTCCTGCCC ACTTCCGAAA GATGTGGGCT CTGGCCACAG GGTTAGGTTC TGAGGGCATC
    CGCCAGGAGT GCCGCTGGGC CTGGGCACAA TGCCAGGACA CCTGGCTGGC ACTGGACCAG
    AAGCTAGAGG CTGTACTGAA GCCACCACTG ACGACGACCA TGACAGTCAG CACGGCTAGC
    CTGTGTGTCA GCAGAGTCCC AGCTGCATCT GCCACCCCTC CCCTGAGGAA GGCATACAGC
    CTGGATCAGA ATTTAGGGCA GAGTCTCAGA CAGCCTGCCC ACCACTGCCA CCACGTGGCC
    ACTGCGGCTG CCTGCCACGG ACCGGAGGCT GGAGGTGGTG CCCAGCCAGG GTCATCCCCT
    ACCATGCCTC TGCCAAGCAC CTCCGACCCC AGGAGCCCCA GCAGGCTCCA GCTGGTGCTG
    GCGGAGATGG TGGCTACGGA GCAGGAGTAT GTCCGTGCTC TTGACTACAC CATAGAGAAC
    TACTTCCCTG AGTTGGATCG TCCTGATGTG CCCCAGGGCC TCCGTGGGCA GCGTGCCCAC
    CTCTTTGGCA ACCTGGAGAA GCTGCGGGAC TTCCACTGTC ATTTCTTCCT GCGTGAACTG
    GAGGCCTGTA CCCAGCATCC ATCCCGGGTA GCCTATGCCT TCCTGCGCCA TAGGGTGCAG
    TTTGGGATGT ATGCACTCTA CAGCAAGAAT AAACCTCGCT CTGATGCCCT GATGACCAGC
    TTCGGTCATG CCTTCTTCAA GGACAAGCAG CAAGCACTGG GGGACCACCT GGACTTGGCC
    TCCTATCTGC TGAAGCCCAT CCAGCGCATG AGCAAGTATG CATTGCTGCT GCAGGAACTG
    GCACGGGCCT GTGGGGGTGC TGTGCAGGAG CTGAGTGCCC TGCGGGCTGC CCAGAGCCTT
    GTGCGCTTCC AGCTGCGACA CGGCAATGAC CTGCTAGCCA TGGATGCCAT TCAGGGCTGT
    GATGTTAACC TCAAGGAACA GGGGCAGCTG GTGCGACAGG ATGAGTTCAT GGTACGCACT
    GGGCGCCGTA AGTCTTTGCG CCGCGTTTTT CTTTTTGAGG AGCTGCTGCT CTTCAGCAAG
    CCCCGCCGAG GACCCACAGG CACCGACACA TTTACCTATA AGCGTTCCTT CAAGATGGCA
    GACCTGGGCC TCACTGAGTG CTGTGGGGAT AGCAACCTGC GGTTTGAGAT CTGGTTCCGC
    CGTCGCAAGG CTAGGGACAC CTTTGTGCTT CAGGCTGCCA GCTTGGCCAC TAAGCAGGCT
    TGGACGGCTG ACATTTCTCG TCTGCTCTGG AGGCAGGCCA TCCACAACAA GGAGGTGCGC
    ATGGCTGAGA TGGTGTCCAT GGGTGTGGGG AACAAGGCCT TCCGGGACCT CGCCCCCAGT
    GAGGAAGCTA TCAACGACCG CACTGTCGAC TATGTCCTGA AGTGCCGAGA AGTTCGCTCT
    CGGGCCTCTA TTGCTGTGGC CCCATTTGAC TGTGACAGCC GCTACCTGGG GGCCTGGAGT
    TCCCTTCCTG GAGACCCCGC CTCTTGCTCT GTACTGGGGT CCCTCAACCT GCACCTGTAC
    AGAGACCCGG CTCTTCTGGG AGTCCGCTGG CCCCTGTATT CTACCAGCGT CCCAGAGGAA
    GCAGCGCTGG AGGCCGAGGT GGAGCTGGGC AGTCAGCTGT CTTTGACTCC TGAGGACTCA
    GAGGTCTCAT CCCAGTGCCC ATCAGTCAGT GGATCCAGTG GCTCTGACAG CAGCTGTGTG
    TCAGGGCAGG TCCTGGGCAG GGGACTTGAG GACTTGTCCT GTGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe36774
    Clone ID Related Accession (Same CDS sequence) XM_014114096.1 , XM_014114096.2
    Accession Version XM_014114096.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3588bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product puratrophin-1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014114096.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGAAGGGC CGCTGGAGGT TGGGGATGAG TCCCCAGACT CCCAGGGACA CGCCACTGAC 
    TGGAGGTTTG CTGTGTGCAG CTTCAGGGAT GCCTGGGATG AAGAGGAATC TGTTGCCCTC
    AAGCAGATTA AGGACTCCAA TCCTCCGGGT CCACCAGCAG GGACAGCCCA GGGGAGCCCC
    CTGCCTGAGG AACTTCAGTC ACCCCCAGGA CAGTTGGCTG TAGACGAGTC GGGAGATGGC
    CAGGGTTGCA CGTTAGAAGG TTCCTCACTC CGGTTAGATG AACCAATCCC CTCTGGAGTG
    GAGAGCCTCC TGTGCCCAAT GTCCTCCTGC CTCAGTCTTG AGCAGGGTGA GAGTGATAGT
    CAAGGGGGAG GCTTGGCAGG GGACCAACTT TCAAGCAGAT TAATGCCAGC CCACCTGGGT
    CCTGGGGGTT TGACCAGTGA TCCGGTGGAC CTTGGAGGCC CATCATCTGA GGTATCATCA
    GAGCTACTGG AGGCAGACCA CAGTGGCTCT AGCCTCCCTA AGCCTGCTGA TTACCTCCGG
    GCCCAAGACC TTGCCTGGGA TCTGCTGGCC AGTGGCATGG CTACCTTGCC AGGGACTCGC
    GATGTAGAAG GCCGGGCAGT GCTGCTTCTG TGTGCCCATA GCCCGGCCTG GCTTCACCTC
    AAGTGCACTA GCCATGAACT CCTCTGCCTC CTGCTCTACC TACGAGGCAT CCCCAGGCCT
    GAAGTACAGG CCCTGGGACT GACTGTTCTG GTGGATGCCC GACTTTGTTC CCCAAGCTCT
    TCCCTCCTCT GGGCGCTCAG CCAACTGCAA GAAGCAGCCC CAGGGTCAGT GTACCAGGTG
    CTGCTACTGG GAAAGATGCC GGAGGAAGTG CCTTCTGGGC TGCAGCTGCA GCAGCTGCCT
    TCTCATCAGA GCCTGCTGAA CCACGTCTCC ACCTCCGGGT TGCCGGCTTC AATGGGAGGA
    GGCCTGCCTT ATTCCCACCA GGCCTGGCTG GACTTCCGGA TGCGTCTGGA AGCCCTACTG
    CAAAGCTGCC AGGTGGCTTG TGCCCTGCTC CAGGGGGCCA TCGAGAGCAT GAAGGCTGTG
    CCCCAGCCTG TAGAGTCTGG GGAAGTTGGT CGGCTGCTAC AGCAGGCACG GGTCCTGATG
    GAGCACGTGC TAGACTCACC ACGGCTAGCA TGGCTACAAT GCCAAGGGGG TTTGGAGCTG
    GCATGGCTGA AGCAGGAAGT CCCAGAGGTG ACCCTGAGCC CAGACTACAG GTCAGCAGTG
    GATGAGGTTG ATGAACTCTA TGGCCGTGTA GATGGACTGC TGCACCAGTT GACCCTCCAG
    AGTAACCGGC GAGTACGGGC ACTAGAGTTG GTCCAGACTC TGGAGGCCCA GGAGGGTGGA
    CTACACCAGA TTGAAGTGTG GCTGCAGCAG GTGGGCTGGC CAGCCCTCGA GGAGCTAAAG
    GAGCCCTCAC TGGACATGCT GCTCCAGGCG AAAGGCCCTT TTCAGGAGCT GGACCAGGTT
    GCCCAGGAGC AGGTCAGGCG AGGGGAGAAG TTTCTACAGC CACTGGCTGG CTGGGAGGCT
    GCTGAACTGG GGCCCGCTGG GGCCCGCTTT CTGGCCCGGC AAGCCAAGCT GACTGACTTC
    TCCAGGGCTT TGACCCGGCG GCGGCAGCAG CTGGCAGATG CAGAGAGGCT ATTCCATCTT
    TTCAAGCAGG CCTCGACATG GGCTGAGGAG GGGCAGCGAG TGTTGGCAGA GCTGGAACAG
    GAGCACCCAG GGGTTGTGCT GCAACGGCTG CAGCTGCATT GGACCAAGCA CCCTGATTTG
    CCTCCTGCCC ACTTCCGAAA GATGTGGGCT CTGGCCACAG GGTTAGGTTC TGAGGGCATC
    CGCCAGGAGT GCCGCTGGGC CTGGGCACAA TGCCAGGACA CCTGGCTGGC ACTGGACCAG
    AAGCTAGAGG CTGTACTGAA GCCACCACTG ACGACGACCA TGACAGTCAG CACGGCTAGC
    CTGTGTGTCA GCAGAGTCCC AGCTGCATCT GCCACCCCTC CCCTGAGGAA GGCATACAGC
    CTGGATCAGA ATTTAGGGCA GAGTCTCAGA CAGCCTGCCC ACCACTGCCA CCACGTGGCC
    ACTGCGGCTG CCTGCCACGG ACCGGAGGCT GGAGGTGGTG CCCAGCCAGG GTCATCCCCT
    ACCATGCCTC TGCCAAGCAC CTCCGACCCC AGGAGCCCCA GCAGGCTCCA GCTGGTGCTG
    GCGGAGATGG TGGCTACGGA GCAGGAGTAT GTCCGTGCTC TTGACTACAC CATAGAGAAC
    TACTTCCCTG AGTTGGATCG TCCTGATGTG CCCCAGGGCC TCCGTGGGCA GCGTGCCCAC
    CTCTTTGGCA ACCTGGAGAA GCTGCGGGAC TTCCACTGTC ATTTCTTCCT GCGTGAACTG
    GAGGCCTGTA CCCAGCATCC ATCCCGGGTA GCCTATGCCT TCCTGCGCCA TAGGGTGCAG
    TTTGGGATGT ATGCACTCTA CAGCAAGAAT AAACCTCGCT CTGATGCCCT GATGACCAGC
    TTCGGTCATG CCTTCTTCAA GGACAAGCAG CAAGCACTGG GGGACCACCT GGACTTGGCC
    TCCTATCTGC TGAAGCCCAT CCAGCGCATG AGCAAGTATG CATTGCTGCT GCAGGAACTG
    GCACGGGCCT GTGGGGGTGC TGTGCAGGAG CTGAGTGCCC TGCGGGCTGC CCAGAGCCTT
    GTGCGCTTCC AGCTGCGACA CGGCAATGAC CTGCTAGCCA TGGATGCCAT TCAGGGCTGT
    GATGTTAACC TCAAGGAACA GGGGCAGCTG GTGCGACAGG ATGAGTTCAT GGTACGCACT
    GGGCGCCGTA AGTCTTTGCG CCGCGTTTTT CTTTTTGAGG AGCTGCTGCT CTTCAGCAAG
    CCCCGCCGAG GACCCACAGG CACCGACACA TTTACCTATA AGCGTTCCTT CAAGATGGCA
    GACCTGGGCC TCACTGAGTG CTGTGGGGAT AGCAACCTGC GGTTTGAGAT CTGGTTCCGC
    CGTCGCAAGG CTAGGGACAC CTTTGTGCTT CAGGCTGCCA GCTTGGCCAC TAAGCAGGCT
    TGGACGGCTG ACATTTCTCG TCTGCTCTGG AGGCAGGCCA TCCACAACAA GGAGGTGCGC
    ATGGCTGAGA TGGTGTCCAT GGGTGTGGGG AACAAGGCCT TCCGGGACCT CGCCCCCAGT
    GAGGAAGCTA TCAACGACCG CACTGTCGAC TATGTCCTGA AGTGCCGAGA AGTTCGCTCT
    CGGGCCTCTA TTGCTGTGGC CCCATTTGAC TGTGACAGCC GCTACCTGGG GGCCTGGAGT
    TCCCTTCCTG GAGACCCCGC CTCTTGCTCT GTACTGGGGT CCCTCAACCT GCACCTGTAC
    AGAGACCCGG CTCTTCTGGG AGTCCGCTGG CCCCTGTATT CTACCAGCGT CCCAGAGGAA
    GCAGCGCTGG AGGCCGAGGT GGAGCTGGGC AGTCAGCTGT CTTTGACTCC TGAGGACTCA
    GAGGTCTCAT CCCAGTGCCC ATCAGTCAGT GGATCCAGTG GCTCTGACAG CAGCTGTGTG
    TCAGGGCAGG TCCTGGGCAG GGGACTTGAG GACTTGTCCT GTGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013969571.1
    CDS402..3989
    Translation

    Target ORF information:

    RefSeq Version XM_014114096.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris pleckstrin homology and RhoGEF domain containing G4 (PLEKHG4), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014114096.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGAAGGGC CGCTGGAGGT TGGGGATGAG TCCCCAGACT CCCAGGGACA CGCCACTGAC 
    TGGAGGTTTG CTGTGTGCAG CTTCAGGGAT GCCTGGGATG AAGAGGAATC TGTTGCCCTC
    AAGCAGATTA AGGACTCCAA TCCTCCGGGT CCACCAGCAG GGACAGCCCA GGGGAGCCCC
    CTGCCTGAGG AACTTCAGTC ACCCCCAGGA CAGTTGGCTG TAGACGAGTC GGGAGATGGC
    CAGGGTTGCA CGTTAGAAGG TTCCTCACTC CGGTTAGATG AACCAATCCC CTCTGGAGTG
    GAGAGCCTCC TGTGCCCAAT GTCCTCCTGC CTCAGTCTTG AGCAGGGTGA GAGTGATAGT
    CAAGGGGGAG GCTTGGCAGG GGACCAACTT TCAAGCAGAT TAATGCCAGC CCACCTGGGT
    CCTGGGGGTT TGACCAGTGA TCCGGTGGAC CTTGGAGGCC CATCATCTGA GGTATCATCA
    GAGCTACTGG AGGCAGACCA CAGTGGCTCT AGCCTCCCTA AGCCTGCTGA TTACCTCCGG
    GCCCAAGACC TTGCCTGGGA TCTGCTGGCC AGTGGCATGG CTACCTTGCC AGGGACTCGC
    GATGTAGAAG GCCGGGCAGT GCTGCTTCTG TGTGCCCATA GCCCGGCCTG GCTTCACCTC
    AAGTGCACTA GCCATGAACT CCTCTGCCTC CTGCTCTACC TACGAGGCAT CCCCAGGCCT
    GAAGTACAGG CCCTGGGACT GACTGTTCTG GTGGATGCCC GACTTTGTTC CCCAAGCTCT
    TCCCTCCTCT GGGCGCTCAG CCAACTGCAA GAAGCAGCCC CAGGGTCAGT GTACCAGGTG
    CTGCTACTGG GAAAGATGCC GGAGGAAGTG CCTTCTGGGC TGCAGCTGCA GCAGCTGCCT
    TCTCATCAGA GCCTGCTGAA CCACGTCTCC ACCTCCGGGT TGCCGGCTTC AATGGGAGGA
    GGCCTGCCTT ATTCCCACCA GGCCTGGCTG GACTTCCGGA TGCGTCTGGA AGCCCTACTG
    CAAAGCTGCC AGGTGGCTTG TGCCCTGCTC CAGGGGGCCA TCGAGAGCAT GAAGGCTGTG
    CCCCAGCCTG TAGAGTCTGG GGAAGTTGGT CGGCTGCTAC AGCAGGCACG GGTCCTGATG
    GAGCACGTGC TAGACTCACC ACGGCTAGCA TGGCTACAAT GCCAAGGGGG TTTGGAGCTG
    GCATGGCTGA AGCAGGAAGT CCCAGAGGTG ACCCTGAGCC CAGACTACAG GTCAGCAGTG
    GATGAGGTTG ATGAACTCTA TGGCCGTGTA GATGGACTGC TGCACCAGTT GACCCTCCAG
    AGTAACCGGC GAGTACGGGC ACTAGAGTTG GTCCAGACTC TGGAGGCCCA GGAGGGTGGA
    CTACACCAGA TTGAAGTGTG GCTGCAGCAG GTGGGCTGGC CAGCCCTCGA GGAGCTAAAG
    GAGCCCTCAC TGGACATGCT GCTCCAGGCG AAAGGCCCTT TTCAGGAGCT GGACCAGGTT
    GCCCAGGAGC AGGTCAGGCG AGGGGAGAAG TTTCTACAGC CACTGGCTGG CTGGGAGGCT
    GCTGAACTGG GGCCCGCTGG GGCCCGCTTT CTGGCCCGGC AAGCCAAGCT GACTGACTTC
    TCCAGGGCTT TGACCCGGCG GCGGCAGCAG CTGGCAGATG CAGAGAGGCT ATTCCATCTT
    TTCAAGCAGG CCTCGACATG GGCTGAGGAG GGGCAGCGAG TGTTGGCAGA GCTGGAACAG
    GAGCACCCAG GGGTTGTGCT GCAACGGCTG CAGCTGCATT GGACCAAGCA CCCTGATTTG
    CCTCCTGCCC ACTTCCGAAA GATGTGGGCT CTGGCCACAG GGTTAGGTTC TGAGGGCATC
    CGCCAGGAGT GCCGCTGGGC CTGGGCACAA TGCCAGGACA CCTGGCTGGC ACTGGACCAG
    AAGCTAGAGG CTGTACTGAA GCCACCACTG ACGACGACCA TGACAGTCAG CACGGCTAGC
    CTGTGTGTCA GCAGAGTCCC AGCTGCATCT GCCACCCCTC CCCTGAGGAA GGCATACAGC
    CTGGATCAGA ATTTAGGGCA GAGTCTCAGA CAGCCTGCCC ACCACTGCCA CCACGTGGCC
    ACTGCGGCTG CCTGCCACGG ACCGGAGGCT GGAGGTGGTG CCCAGCCAGG GTCATCCCCT
    ACCATGCCTC TGCCAAGCAC CTCCGACCCC AGGAGCCCCA GCAGGCTCCA GCTGGTGCTG
    GCGGAGATGG TGGCTACGGA GCAGGAGTAT GTCCGTGCTC TTGACTACAC CATAGAGAAC
    TACTTCCCTG AGTTGGATCG TCCTGATGTG CCCCAGGGCC TCCGTGGGCA GCGTGCCCAC
    CTCTTTGGCA ACCTGGAGAA GCTGCGGGAC TTCCACTGTC ATTTCTTCCT GCGTGAACTG
    GAGGCCTGTA CCCAGCATCC ATCCCGGGTA GCCTATGCCT TCCTGCGCCA TAGGGTGCAG
    TTTGGGATGT ATGCACTCTA CAGCAAGAAT AAACCTCGCT CTGATGCCCT GATGACCAGC
    TTCGGTCATG CCTTCTTCAA GGACAAGCAG CAAGCACTGG GGGACCACCT GGACTTGGCC
    TCCTATCTGC TGAAGCCCAT CCAGCGCATG AGCAAGTATG CATTGCTGCT GCAGGAACTG
    GCACGGGCCT GTGGGGGTGC TGTGCAGGAG CTGAGTGCCC TGCGGGCTGC CCAGAGCCTT
    GTGCGCTTCC AGCTGCGACA CGGCAATGAC CTGCTAGCCA TGGATGCCAT TCAGGGCTGT
    GATGTTAACC TCAAGGAACA GGGGCAGCTG GTGCGACAGG ATGAGTTCAT GGTACGCACT
    GGGCGCCGTA AGTCTTTGCG CCGCGTTTTT CTTTTTGAGG AGCTGCTGCT CTTCAGCAAG
    CCCCGCCGAG GACCCACAGG CACCGACACA TTTACCTATA AGCGTTCCTT CAAGATGGCA
    GACCTGGGCC TCACTGAGTG CTGTGGGGAT AGCAACCTGC GGTTTGAGAT CTGGTTCCGC
    CGTCGCAAGG CTAGGGACAC CTTTGTGCTT CAGGCTGCCA GCTTGGCCAC TAAGCAGGCT
    TGGACGGCTG ACATTTCTCG TCTGCTCTGG AGGCAGGCCA TCCACAACAA GGAGGTGCGC
    ATGGCTGAGA TGGTGTCCAT GGGTGTGGGG AACAAGGCCT TCCGGGACCT CGCCCCCAGT
    GAGGAAGCTA TCAACGACCG CACTGTCGAC TATGTCCTGA AGTGCCGAGA AGTTCGCTCT
    CGGGCCTCTA TTGCTGTGGC CCCATTTGAC TGTGACAGCC GCTACCTGGG GGCCTGGAGT
    TCCCTTCCTG GAGACCCCGC CTCTTGCTCT GTACTGGGGT CCCTCAACCT GCACCTGTAC
    AGAGACCCGG CTCTTCTGGG AGTCCGCTGG CCCCTGTATT CTACCAGCGT CCCAGAGGAA
    GCAGCGCTGG AGGCCGAGGT GGAGCTGGGC AGTCAGCTGT CTTTGACTCC TGAGGACTCA
    GAGGTCTCAT CCCAGTGCCC ATCAGTCAGT GGATCCAGTG GCTCTGACAG CAGCTGTGTG
    TCAGGGCAGG TCCTGGGCAG GGGACTTGAG GACTTGTCCT GTGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe07253
    Clone ID Related Accession (Same CDS sequence) XM_022419321.1 , XM_014114094.2 , XM_005620823.2 , XM_014114094.1 , XM_005620823.3
    Accession Version XM_022419321.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3642bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product puratrophin-1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGAAGGGC CGCTGGAGGT TGGGGATGAG TCCCCAGACT CCCAGGGACA CGCCACTGAC 
    TGGAGGTTTG CTGTGTGCAG CTTCAGGGAT GCCTGGGATG AAGAGGAATC TGTTGCCCTC
    AAGCAGATTA AGGACTCCAA TCCTCCGGGT CCACCAGCAG GGACAGCCCA GGGGAGCCCC
    CTGCCTGAGG AACTTCAGTC ACCCCCAGGA CAGTTGGCTG TAGACGAGTC GGGAGATGGC
    CAGGGTTGCA CGTTAGAAGG TTCCTCACTC CGGTTAGATG AACCAATCCC CTCTGGAGTG
    GAGAGCCTCC TGTGCCCAAT GTCCTCCTGC CTCAGTCTTG AGCAGGGTGA GAGTGATAGT
    CAAGGGGGAG GCTTGGCAGG GGACCAACTT TCAAGCAGAT TAATGCCAGC CCACCTGGGT
    CCTGGGGGTT TGACCAGTGA TCCGGTGGAC CTTGGAGGCC CATCATCTGA GGTATCATCA
    GAGCTACTGG AGGCAGACCA CAGTGGCTCT AGCCTCCCTA AGCCTGCTGA TTACCTCCGG
    GCCCAAGACC TTGCCTGGGA TCTGCTGGCC AGTGGCATGG CTACCTTGCC AGGGACTCGC
    GATGTAGAAG GCCGGGCAGT GCTGCTTCTG TGTGCCCATA GCCCGGCCTG GCTTCACCTC
    AAGTGCACTA GCCATGAACT CCTCTGCCTC CTGCTCTACC TACGAGGCAT CCCCAGGCCT
    GAAGTACAGG CCCTGGGACT GACTGTTCTG GTGGATGCCC GACTTTGTTC CCCAAGCTCT
    TCCCTCCTCT GGGCGCTCAG CCAACTGCAA GAAGCAGCCC CAGGGTCAGT GTACCAGGTG
    CTGCTACTGG GAAAGATGCC GGAGGAAGTG CCTTCTGGGC TGCAGCTGCA GCAGCTGCCT
    TCTCATCAGA GCCTGCTGAA CCACGTCTCC ACCTCCGGGT TGCCGGCTTC AATGGGAGGA
    GGCCTGCCTT ATTCCCACCA GGCCTGGCTG GACTTCCGGA TGCGTCTGGA AGCCCTACTG
    CAAAGCTGCC AGGTGGCTTG TGCCCTGCTC CAGGGGGCCA TCGAGAGCAT GAAGGCTGTG
    CCCCAGCCTG TAGAGTCTGG GGAAGTTGGT CGGCTGCTAC AGCAGGCACG GGTCCTGATG
    GAGCACGTGC TAGACTCACC ACGGCTAGCA TGGCTACAAT GCCAAGGGGG TTTGGAGCTG
    GCATGGCTGA AGCAGGAAGT CCCAGAGGTG ACCCTGAGCC CAGACTACAG GTCAGCAGTG
    GATGAGGTTG ATGAACTCTA TGGCCGTGTA GATGGACTGC TGCACCAGTT GACCCTCCAG
    AGTAACCGGC GAGTACGGGC ACTAGAGTTG GTCCAGACTC TGGAGGCCCA GGAGGGTGGA
    CTACACCAGA TTGAAGTGTG GCTGCAGCAG GTGGGCTGGC CAGCCCTCGA GGAGCTAAAG
    GAGCCCTCAC TGGACATGCT GCTCCAGGCG AAAGGCCCTT TTCAGGAGCT GGACCAGGTT
    GCCCAGGAGC AGGTCAGGCG AGGGGAGAAG TTTCTACAGC CACTGGCTGG CTGGGAGGCT
    GCTGAACTGG GGCCCGCTGG GGCCCGCTTT CTGGCCCGGC AAGCCAAGCT GACTGACTTC
    TCCAGGGCTT TGACCCGGCG GCGGCAGCAG CTGGCAGATG CAGAGAGGCT ATTCCATCTT
    TTCAAGCAGG CCTCGACATG GGCTGAGGAG GGGCAGCGAG TGTTGGCAGA GCTGGAACAG
    GAGCACCCAG GGGTTGTGCT GCAACGGCTG CAGCTGCATT GGACCAAGCA CCCTGATTTG
    CCTCCTGCCC ACTTCCGAAA GATGTGGGCT CTGGCCACAG GGTTAGGTTC TGAGGGCATC
    CGCCAGGAGT GCCGCTGGGC CTGGGCACAA TGCCAGGACA CCTGGCTGGC ACTGGACCAG
    AAGCTAGAGG CTGTACTGAA GCCACCACTG ACGACGACCA TGACAGTCAG CACGGCTAGC
    CTGTGTGTCA GCAGAGTCCC AGCTGCATCT GCCACCCCTC CCCTGAGGAA GGCATACAGC
    CTGGATCAGA ATTTAGGGCA GAGTCTCAGA CAGCCTGCCC ACCACTGCCA CCACGTGGCC
    ACTGCGGCTG CCTGCCACGG ACCGGAGGCT GGAGGTGGTG CCCAGCCAGG GTCATCCCCT
    ACCATGCCTC TGCCAAGCAC CTCCGACCCC AGGAGCCCCA GCAGGCTCCA GCTGGTGCTG
    GCGGAGATGG TGGCTACGGA GCAGGAGTAT GTCCGTGCTC TTGACTACAC CATAGAGAAC
    TACTTCCCTG AGTTGGATCG TCCTGATGTG CCCCAGGGCC TCCGTGGGCA GCGTGCCCAC
    CTCTTTGGCA ACCTGGAGAA GCTGCGGGAC TTCCACTGTC ATTTCTTCCT GCGTGAACTG
    GAGGCCTGTA CCCAGCATCC ATCCCGGGTA GCCTATGCCT TCCTGCGCCA TAGGGTGCAG
    TTTGGGATGT ATGCACTCTA CAGCAAGAAT AAACCTCGCT CTGATGCCCT GATGACCAGC
    TTCGGTCATG CCTTCTTCAA GGACAAGCAG CAAGCACTGG GGGACCACCT GGACTTGGCC
    TCCTATCTGC TGAAGCCCAT CCAGCGCATG AGCAAGTATG CATTGCTGCT GCAGGAACTG
    GCACGGGCCT GTGGGGGTGC TGTGCAGGAG CTGAGTGCCC TGCGGGCTGC CCAGAGCCTT
    GTGCGCTTCC AGCTGCGACA CGGCAATGAC CTGCTAGCCA TGGATGCCAT TCAGGGCTGT
    GATGTTAACC TCAAGGAACA GGGGCAGCTG GTGCGACAGG ATGAGTTCAT GGTACGCACT
    GGGCGCCGTA AGTCTTTGCG CCGCGTTTTT CTTTTTGAGG AGCTGCTGCT CTTCAGCAAG
    CCCCGCCGAG GACCCACAGG CACCGACACA TTTACCTATA AGCGTTCCTT CAAGATGGCA
    GACCTGGGCC TCACTGAGTG CTGTGGGGAT AGCAACCTGC GGTTTGAGAT CTGGTTCCGC
    CGTCGCAAGG CTAGGGACAC CTTTGTGCTT CAGGCTGCCA GCTTGGCCAC TAAGCAGGCT
    TGGACGGCTG ACATTTCTCG TCTGCTCTGG AGGCAGGCCA TCCACAACAA GGAGGTGCGC
    ATGGCTGAGA TGGTGTCCAT GGGTGTGGGG AACAAGGCCT TCCGGGACCT CGCCCCCAGT
    GAGGAAGCTA TCAACGACCG CACTGTCGAC TATGTCCTGA AGTGCCGAGA AGTTCGCTCT
    CGGGCCTCTA TTGCTGTGGC CCCATTTGAC TGTGACAGCC GCTACCTGGG GGCCTGGAGT
    TCCCTTCCTG GAGACCCCGC CTCTTGCTCT GTACTGGGGT CCCTCAACCT GCACCTGTAC
    AGAGACCCGG CTCTTCTGGG AGTCCGCTGG CCCCTGTATT CTACCAGCGT CCCAGAGGAA
    GCAGCGCTGG AGGCCGAGGT GGAGCTGGGC AGTCAGCTGT CTTTGACTCC TGAGGACTCA
    GAGGTCTCAT CCCAGTGCCC ATCAGTCAGT GGATCCAGTG GCTCTGACAG CAGCTGTGTG
    TCAGGGCAGG TCCTGGGCAG GGGACTTGAG GACTTGTCCT GTATTCAAAT GCTGAAATTC
    TGCCCCAGTG GAAGAAACAA TAATGCCCTT GAACAACTCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022275029.1
    CDS320..3961
    Translation

    Target ORF information:

    RefSeq Version XM_022419321.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris pleckstrin homology and RhoGEF domain containing G4 (PLEKHG4), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022419321.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGAAGGGC CGCTGGAGGT TGGGGATGAG TCCCCAGACT CCCAGGGACA CGCCACTGAC 
    TGGAGGTTTG CTGTGTGCAG CTTCAGGGAT GCCTGGGATG AAGAGGAATC TGTTGCCCTC
    AAGCAGATTA AGGACTCCAA TCCTCCGGGT CCACCAGCAG GGACAGCCCA GGGGAGCCCC
    CTGCCTGAGG AACTTCAGTC ACCCCCAGGA CAGTTGGCTG TAGACGAGTC GGGAGATGGC
    CAGGGTTGCA CGTTAGAAGG TTCCTCACTC CGGTTAGATG AACCAATCCC CTCTGGAGTG
    GAGAGCCTCC TGTGCCCAAT GTCCTCCTGC CTCAGTCTTG AGCAGGGTGA GAGTGATAGT
    CAAGGGGGAG GCTTGGCAGG GGACCAACTT TCAAGCAGAT TAATGCCAGC CCACCTGGGT
    CCTGGGGGTT TGACCAGTGA TCCGGTGGAC CTTGGAGGCC CATCATCTGA GGTATCATCA
    GAGCTACTGG AGGCAGACCA CAGTGGCTCT AGCCTCCCTA AGCCTGCTGA TTACCTCCGG
    GCCCAAGACC TTGCCTGGGA TCTGCTGGCC AGTGGCATGG CTACCTTGCC AGGGACTCGC
    GATGTAGAAG GCCGGGCAGT GCTGCTTCTG TGTGCCCATA GCCCGGCCTG GCTTCACCTC
    AAGTGCACTA GCCATGAACT CCTCTGCCTC CTGCTCTACC TACGAGGCAT CCCCAGGCCT
    GAAGTACAGG CCCTGGGACT GACTGTTCTG GTGGATGCCC GACTTTGTTC CCCAAGCTCT
    TCCCTCCTCT GGGCGCTCAG CCAACTGCAA GAAGCAGCCC CAGGGTCAGT GTACCAGGTG
    CTGCTACTGG GAAAGATGCC GGAGGAAGTG CCTTCTGGGC TGCAGCTGCA GCAGCTGCCT
    TCTCATCAGA GCCTGCTGAA CCACGTCTCC ACCTCCGGGT TGCCGGCTTC AATGGGAGGA
    GGCCTGCCTT ATTCCCACCA GGCCTGGCTG GACTTCCGGA TGCGTCTGGA AGCCCTACTG
    CAAAGCTGCC AGGTGGCTTG TGCCCTGCTC CAGGGGGCCA TCGAGAGCAT GAAGGCTGTG
    CCCCAGCCTG TAGAGTCTGG GGAAGTTGGT CGGCTGCTAC AGCAGGCACG GGTCCTGATG
    GAGCACGTGC TAGACTCACC ACGGCTAGCA TGGCTACAAT GCCAAGGGGG TTTGGAGCTG
    GCATGGCTGA AGCAGGAAGT CCCAGAGGTG ACCCTGAGCC CAGACTACAG GTCAGCAGTG
    GATGAGGTTG ATGAACTCTA TGGCCGTGTA GATGGACTGC TGCACCAGTT GACCCTCCAG
    AGTAACCGGC GAGTACGGGC ACTAGAGTTG GTCCAGACTC TGGAGGCCCA GGAGGGTGGA
    CTACACCAGA TTGAAGTGTG GCTGCAGCAG GTGGGCTGGC CAGCCCTCGA GGAGCTAAAG
    GAGCCCTCAC TGGACATGCT GCTCCAGGCG AAAGGCCCTT TTCAGGAGCT GGACCAGGTT
    GCCCAGGAGC AGGTCAGGCG AGGGGAGAAG TTTCTACAGC CACTGGCTGG CTGGGAGGCT
    GCTGAACTGG GGCCCGCTGG GGCCCGCTTT CTGGCCCGGC AAGCCAAGCT GACTGACTTC
    TCCAGGGCTT TGACCCGGCG GCGGCAGCAG CTGGCAGATG CAGAGAGGCT ATTCCATCTT
    TTCAAGCAGG CCTCGACATG GGCTGAGGAG GGGCAGCGAG TGTTGGCAGA GCTGGAACAG
    GAGCACCCAG GGGTTGTGCT GCAACGGCTG CAGCTGCATT GGACCAAGCA CCCTGATTTG
    CCTCCTGCCC ACTTCCGAAA GATGTGGGCT CTGGCCACAG GGTTAGGTTC TGAGGGCATC
    CGCCAGGAGT GCCGCTGGGC CTGGGCACAA TGCCAGGACA CCTGGCTGGC ACTGGACCAG
    AAGCTAGAGG CTGTACTGAA GCCACCACTG ACGACGACCA TGACAGTCAG CACGGCTAGC
    CTGTGTGTCA GCAGAGTCCC AGCTGCATCT GCCACCCCTC CCCTGAGGAA GGCATACAGC
    CTGGATCAGA ATTTAGGGCA GAGTCTCAGA CAGCCTGCCC ACCACTGCCA CCACGTGGCC
    ACTGCGGCTG CCTGCCACGG ACCGGAGGCT GGAGGTGGTG CCCAGCCAGG GTCATCCCCT
    ACCATGCCTC TGCCAAGCAC CTCCGACCCC AGGAGCCCCA GCAGGCTCCA GCTGGTGCTG
    GCGGAGATGG TGGCTACGGA GCAGGAGTAT GTCCGTGCTC TTGACTACAC CATAGAGAAC
    TACTTCCCTG AGTTGGATCG TCCTGATGTG CCCCAGGGCC TCCGTGGGCA GCGTGCCCAC
    CTCTTTGGCA ACCTGGAGAA GCTGCGGGAC TTCCACTGTC ATTTCTTCCT GCGTGAACTG
    GAGGCCTGTA CCCAGCATCC ATCCCGGGTA GCCTATGCCT TCCTGCGCCA TAGGGTGCAG
    TTTGGGATGT ATGCACTCTA CAGCAAGAAT AAACCTCGCT CTGATGCCCT GATGACCAGC
    TTCGGTCATG CCTTCTTCAA GGACAAGCAG CAAGCACTGG GGGACCACCT GGACTTGGCC
    TCCTATCTGC TGAAGCCCAT CCAGCGCATG AGCAAGTATG CATTGCTGCT GCAGGAACTG
    GCACGGGCCT GTGGGGGTGC TGTGCAGGAG CTGAGTGCCC TGCGGGCTGC CCAGAGCCTT
    GTGCGCTTCC AGCTGCGACA CGGCAATGAC CTGCTAGCCA TGGATGCCAT TCAGGGCTGT
    GATGTTAACC TCAAGGAACA GGGGCAGCTG GTGCGACAGG ATGAGTTCAT GGTACGCACT
    GGGCGCCGTA AGTCTTTGCG CCGCGTTTTT CTTTTTGAGG AGCTGCTGCT CTTCAGCAAG
    CCCCGCCGAG GACCCACAGG CACCGACACA TTTACCTATA AGCGTTCCTT CAAGATGGCA
    GACCTGGGCC TCACTGAGTG CTGTGGGGAT AGCAACCTGC GGTTTGAGAT CTGGTTCCGC
    CGTCGCAAGG CTAGGGACAC CTTTGTGCTT CAGGCTGCCA GCTTGGCCAC TAAGCAGGCT
    TGGACGGCTG ACATTTCTCG TCTGCTCTGG AGGCAGGCCA TCCACAACAA GGAGGTGCGC
    ATGGCTGAGA TGGTGTCCAT GGGTGTGGGG AACAAGGCCT TCCGGGACCT CGCCCCCAGT
    GAGGAAGCTA TCAACGACCG CACTGTCGAC TATGTCCTGA AGTGCCGAGA AGTTCGCTCT
    CGGGCCTCTA TTGCTGTGGC CCCATTTGAC TGTGACAGCC GCTACCTGGG GGCCTGGAGT
    TCCCTTCCTG GAGACCCCGC CTCTTGCTCT GTACTGGGGT CCCTCAACCT GCACCTGTAC
    AGAGACCCGG CTCTTCTGGG AGTCCGCTGG CCCCTGTATT CTACCAGCGT CCCAGAGGAA
    GCAGCGCTGG AGGCCGAGGT GGAGCTGGGC AGTCAGCTGT CTTTGACTCC TGAGGACTCA
    GAGGTCTCAT CCCAGTGCCC ATCAGTCAGT GGATCCAGTG GCTCTGACAG CAGCTGTGTG
    TCAGGGCAGG TCCTGGGCAG GGGACTTGAG GACTTGTCCT GTATTCAAAT GCTGAAATTC
    TGCCCCAGTG GAAGAAACAA TAATGCCCTT GAACAACTCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe07253
    Clone ID Related Accession (Same CDS sequence) XM_022419321.1 , XM_014114094.2 , XM_005620823.2 , XM_014114094.1 , XM_005620823.3
    Accession Version XM_014114094.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3642bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product puratrophin-1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014114094.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGAAGGGC CGCTGGAGGT TGGGGATGAG TCCCCAGACT CCCAGGGACA CGCCACTGAC 
    TGGAGGTTTG CTGTGTGCAG CTTCAGGGAT GCCTGGGATG AAGAGGAATC TGTTGCCCTC
    AAGCAGATTA AGGACTCCAA TCCTCCGGGT CCACCAGCAG GGACAGCCCA GGGGAGCCCC
    CTGCCTGAGG AACTTCAGTC ACCCCCAGGA CAGTTGGCTG TAGACGAGTC GGGAGATGGC
    CAGGGTTGCA CGTTAGAAGG TTCCTCACTC CGGTTAGATG AACCAATCCC CTCTGGAGTG
    GAGAGCCTCC TGTGCCCAAT GTCCTCCTGC CTCAGTCTTG AGCAGGGTGA GAGTGATAGT
    CAAGGGGGAG GCTTGGCAGG GGACCAACTT TCAAGCAGAT TAATGCCAGC CCACCTGGGT
    CCTGGGGGTT TGACCAGTGA TCCGGTGGAC CTTGGAGGCC CATCATCTGA GGTATCATCA
    GAGCTACTGG AGGCAGACCA CAGTGGCTCT AGCCTCCCTA AGCCTGCTGA TTACCTCCGG
    GCCCAAGACC TTGCCTGGGA TCTGCTGGCC AGTGGCATGG CTACCTTGCC AGGGACTCGC
    GATGTAGAAG GCCGGGCAGT GCTGCTTCTG TGTGCCCATA GCCCGGCCTG GCTTCACCTC
    AAGTGCACTA GCCATGAACT CCTCTGCCTC CTGCTCTACC TACGAGGCAT CCCCAGGCCT
    GAAGTACAGG CCCTGGGACT GACTGTTCTG GTGGATGCCC GACTTTGTTC CCCAAGCTCT
    TCCCTCCTCT GGGCGCTCAG CCAACTGCAA GAAGCAGCCC CAGGGTCAGT GTACCAGGTG
    CTGCTACTGG GAAAGATGCC GGAGGAAGTG CCTTCTGGGC TGCAGCTGCA GCAGCTGCCT
    TCTCATCAGA GCCTGCTGAA CCACGTCTCC ACCTCCGGGT TGCCGGCTTC AATGGGAGGA
    GGCCTGCCTT ATTCCCACCA GGCCTGGCTG GACTTCCGGA TGCGTCTGGA AGCCCTACTG
    CAAAGCTGCC AGGTGGCTTG TGCCCTGCTC CAGGGGGCCA TCGAGAGCAT GAAGGCTGTG
    CCCCAGCCTG TAGAGTCTGG GGAAGTTGGT CGGCTGCTAC AGCAGGCACG GGTCCTGATG
    GAGCACGTGC TAGACTCACC ACGGCTAGCA TGGCTACAAT GCCAAGGGGG TTTGGAGCTG
    GCATGGCTGA AGCAGGAAGT CCCAGAGGTG ACCCTGAGCC CAGACTACAG GTCAGCAGTG
    GATGAGGTTG ATGAACTCTA TGGCCGTGTA GATGGACTGC TGCACCAGTT GACCCTCCAG
    AGTAACCGGC GAGTACGGGC ACTAGAGTTG GTCCAGACTC TGGAGGCCCA GGAGGGTGGA
    CTACACCAGA TTGAAGTGTG GCTGCAGCAG GTGGGCTGGC CAGCCCTCGA GGAGCTAAAG
    GAGCCCTCAC TGGACATGCT GCTCCAGGCG AAAGGCCCTT TTCAGGAGCT GGACCAGGTT
    GCCCAGGAGC AGGTCAGGCG AGGGGAGAAG TTTCTACAGC CACTGGCTGG CTGGGAGGCT
    GCTGAACTGG GGCCCGCTGG GGCCCGCTTT CTGGCCCGGC AAGCCAAGCT GACTGACTTC
    TCCAGGGCTT TGACCCGGCG GCGGCAGCAG CTGGCAGATG CAGAGAGGCT ATTCCATCTT
    TTCAAGCAGG CCTCGACATG GGCTGAGGAG GGGCAGCGAG TGTTGGCAGA GCTGGAACAG
    GAGCACCCAG GGGTTGTGCT GCAACGGCTG CAGCTGCATT GGACCAAGCA CCCTGATTTG
    CCTCCTGCCC ACTTCCGAAA GATGTGGGCT CTGGCCACAG GGTTAGGTTC TGAGGGCATC
    CGCCAGGAGT GCCGCTGGGC CTGGGCACAA TGCCAGGACA CCTGGCTGGC ACTGGACCAG
    AAGCTAGAGG CTGTACTGAA GCCACCACTG ACGACGACCA TGACAGTCAG CACGGCTAGC
    CTGTGTGTCA GCAGAGTCCC AGCTGCATCT GCCACCCCTC CCCTGAGGAA GGCATACAGC
    CTGGATCAGA ATTTAGGGCA GAGTCTCAGA CAGCCTGCCC ACCACTGCCA CCACGTGGCC
    ACTGCGGCTG CCTGCCACGG ACCGGAGGCT GGAGGTGGTG CCCAGCCAGG GTCATCCCCT
    ACCATGCCTC TGCCAAGCAC CTCCGACCCC AGGAGCCCCA GCAGGCTCCA GCTGGTGCTG
    GCGGAGATGG TGGCTACGGA GCAGGAGTAT GTCCGTGCTC TTGACTACAC CATAGAGAAC
    TACTTCCCTG AGTTGGATCG TCCTGATGTG CCCCAGGGCC TCCGTGGGCA GCGTGCCCAC
    CTCTTTGGCA ACCTGGAGAA GCTGCGGGAC TTCCACTGTC ATTTCTTCCT GCGTGAACTG
    GAGGCCTGTA CCCAGCATCC ATCCCGGGTA GCCTATGCCT TCCTGCGCCA TAGGGTGCAG
    TTTGGGATGT ATGCACTCTA CAGCAAGAAT AAACCTCGCT CTGATGCCCT GATGACCAGC
    TTCGGTCATG CCTTCTTCAA GGACAAGCAG CAAGCACTGG GGGACCACCT GGACTTGGCC
    TCCTATCTGC TGAAGCCCAT CCAGCGCATG AGCAAGTATG CATTGCTGCT GCAGGAACTG
    GCACGGGCCT GTGGGGGTGC TGTGCAGGAG CTGAGTGCCC TGCGGGCTGC CCAGAGCCTT
    GTGCGCTTCC AGCTGCGACA CGGCAATGAC CTGCTAGCCA TGGATGCCAT TCAGGGCTGT
    GATGTTAACC TCAAGGAACA GGGGCAGCTG GTGCGACAGG ATGAGTTCAT GGTACGCACT
    GGGCGCCGTA AGTCTTTGCG CCGCGTTTTT CTTTTTGAGG AGCTGCTGCT CTTCAGCAAG
    CCCCGCCGAG GACCCACAGG CACCGACACA TTTACCTATA AGCGTTCCTT CAAGATGGCA
    GACCTGGGCC TCACTGAGTG CTGTGGGGAT AGCAACCTGC GGTTTGAGAT CTGGTTCCGC
    CGTCGCAAGG CTAGGGACAC CTTTGTGCTT CAGGCTGCCA GCTTGGCCAC TAAGCAGGCT
    TGGACGGCTG ACATTTCTCG TCTGCTCTGG AGGCAGGCCA TCCACAACAA GGAGGTGCGC
    ATGGCTGAGA TGGTGTCCAT GGGTGTGGGG AACAAGGCCT TCCGGGACCT CGCCCCCAGT
    GAGGAAGCTA TCAACGACCG CACTGTCGAC TATGTCCTGA AGTGCCGAGA AGTTCGCTCT
    CGGGCCTCTA TTGCTGTGGC CCCATTTGAC TGTGACAGCC GCTACCTGGG GGCCTGGAGT
    TCCCTTCCTG GAGACCCCGC CTCTTGCTCT GTACTGGGGT CCCTCAACCT GCACCTGTAC
    AGAGACCCGG CTCTTCTGGG AGTCCGCTGG CCCCTGTATT CTACCAGCGT CCCAGAGGAA
    GCAGCGCTGG AGGCCGAGGT GGAGCTGGGC AGTCAGCTGT CTTTGACTCC TGAGGACTCA
    GAGGTCTCAT CCCAGTGCCC ATCAGTCAGT GGATCCAGTG GCTCTGACAG CAGCTGTGTG
    TCAGGGCAGG TCCTGGGCAG GGGACTTGAG GACTTGTCCT GTATTCAAAT GCTGAAATTC
    TGCCCCAGTG GAAGAAACAA TAATGCCCTT GAACAACTCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013969569.1
    CDS355..3996
    Translation

    Target ORF information:

    RefSeq Version XM_014114094.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris pleckstrin homology and RhoGEF domain containing G4 (PLEKHG4), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014114094.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGAAGGGC CGCTGGAGGT TGGGGATGAG TCCCCAGACT CCCAGGGACA CGCCACTGAC 
    TGGAGGTTTG CTGTGTGCAG CTTCAGGGAT GCCTGGGATG AAGAGGAATC TGTTGCCCTC
    AAGCAGATTA AGGACTCCAA TCCTCCGGGT CCACCAGCAG GGACAGCCCA GGGGAGCCCC
    CTGCCTGAGG AACTTCAGTC ACCCCCAGGA CAGTTGGCTG TAGACGAGTC GGGAGATGGC
    CAGGGTTGCA CGTTAGAAGG TTCCTCACTC CGGTTAGATG AACCAATCCC CTCTGGAGTG
    GAGAGCCTCC TGTGCCCAAT GTCCTCCTGC CTCAGTCTTG AGCAGGGTGA GAGTGATAGT
    CAAGGGGGAG GCTTGGCAGG GGACCAACTT TCAAGCAGAT TAATGCCAGC CCACCTGGGT
    CCTGGGGGTT TGACCAGTGA TCCGGTGGAC CTTGGAGGCC CATCATCTGA GGTATCATCA
    GAGCTACTGG AGGCAGACCA CAGTGGCTCT AGCCTCCCTA AGCCTGCTGA TTACCTCCGG
    GCCCAAGACC TTGCCTGGGA TCTGCTGGCC AGTGGCATGG CTACCTTGCC AGGGACTCGC
    GATGTAGAAG GCCGGGCAGT GCTGCTTCTG TGTGCCCATA GCCCGGCCTG GCTTCACCTC
    AAGTGCACTA GCCATGAACT CCTCTGCCTC CTGCTCTACC TACGAGGCAT CCCCAGGCCT
    GAAGTACAGG CCCTGGGACT GACTGTTCTG GTGGATGCCC GACTTTGTTC CCCAAGCTCT
    TCCCTCCTCT GGGCGCTCAG CCAACTGCAA GAAGCAGCCC CAGGGTCAGT GTACCAGGTG
    CTGCTACTGG GAAAGATGCC GGAGGAAGTG CCTTCTGGGC TGCAGCTGCA GCAGCTGCCT
    TCTCATCAGA GCCTGCTGAA CCACGTCTCC ACCTCCGGGT TGCCGGCTTC AATGGGAGGA
    GGCCTGCCTT ATTCCCACCA GGCCTGGCTG GACTTCCGGA TGCGTCTGGA AGCCCTACTG
    CAAAGCTGCC AGGTGGCTTG TGCCCTGCTC CAGGGGGCCA TCGAGAGCAT GAAGGCTGTG
    CCCCAGCCTG TAGAGTCTGG GGAAGTTGGT CGGCTGCTAC AGCAGGCACG GGTCCTGATG
    GAGCACGTGC TAGACTCACC ACGGCTAGCA TGGCTACAAT GCCAAGGGGG TTTGGAGCTG
    GCATGGCTGA AGCAGGAAGT CCCAGAGGTG ACCCTGAGCC CAGACTACAG GTCAGCAGTG
    GATGAGGTTG ATGAACTCTA TGGCCGTGTA GATGGACTGC TGCACCAGTT GACCCTCCAG
    AGTAACCGGC GAGTACGGGC ACTAGAGTTG GTCCAGACTC TGGAGGCCCA GGAGGGTGGA
    CTACACCAGA TTGAAGTGTG GCTGCAGCAG GTGGGCTGGC CAGCCCTCGA GGAGCTAAAG
    GAGCCCTCAC TGGACATGCT GCTCCAGGCG AAAGGCCCTT TTCAGGAGCT GGACCAGGTT
    GCCCAGGAGC AGGTCAGGCG AGGGGAGAAG TTTCTACAGC CACTGGCTGG CTGGGAGGCT
    GCTGAACTGG GGCCCGCTGG GGCCCGCTTT CTGGCCCGGC AAGCCAAGCT GACTGACTTC
    TCCAGGGCTT TGACCCGGCG GCGGCAGCAG CTGGCAGATG CAGAGAGGCT ATTCCATCTT
    TTCAAGCAGG CCTCGACATG GGCTGAGGAG GGGCAGCGAG TGTTGGCAGA GCTGGAACAG
    GAGCACCCAG GGGTTGTGCT GCAACGGCTG CAGCTGCATT GGACCAAGCA CCCTGATTTG
    CCTCCTGCCC ACTTCCGAAA GATGTGGGCT CTGGCCACAG GGTTAGGTTC TGAGGGCATC
    CGCCAGGAGT GCCGCTGGGC CTGGGCACAA TGCCAGGACA CCTGGCTGGC ACTGGACCAG
    AAGCTAGAGG CTGTACTGAA GCCACCACTG ACGACGACCA TGACAGTCAG CACGGCTAGC
    CTGTGTGTCA GCAGAGTCCC AGCTGCATCT GCCACCCCTC CCCTGAGGAA GGCATACAGC
    CTGGATCAGA ATTTAGGGCA GAGTCTCAGA CAGCCTGCCC ACCACTGCCA CCACGTGGCC
    ACTGCGGCTG CCTGCCACGG ACCGGAGGCT GGAGGTGGTG CCCAGCCAGG GTCATCCCCT
    ACCATGCCTC TGCCAAGCAC CTCCGACCCC AGGAGCCCCA GCAGGCTCCA GCTGGTGCTG
    GCGGAGATGG TGGCTACGGA GCAGGAGTAT GTCCGTGCTC TTGACTACAC CATAGAGAAC
    TACTTCCCTG AGTTGGATCG TCCTGATGTG CCCCAGGGCC TCCGTGGGCA GCGTGCCCAC
    CTCTTTGGCA ACCTGGAGAA GCTGCGGGAC TTCCACTGTC ATTTCTTCCT GCGTGAACTG
    GAGGCCTGTA CCCAGCATCC ATCCCGGGTA GCCTATGCCT TCCTGCGCCA TAGGGTGCAG
    TTTGGGATGT ATGCACTCTA CAGCAAGAAT AAACCTCGCT CTGATGCCCT GATGACCAGC
    TTCGGTCATG CCTTCTTCAA GGACAAGCAG CAAGCACTGG GGGACCACCT GGACTTGGCC
    TCCTATCTGC TGAAGCCCAT CCAGCGCATG AGCAAGTATG CATTGCTGCT GCAGGAACTG
    GCACGGGCCT GTGGGGGTGC TGTGCAGGAG CTGAGTGCCC TGCGGGCTGC CCAGAGCCTT
    GTGCGCTTCC AGCTGCGACA CGGCAATGAC CTGCTAGCCA TGGATGCCAT TCAGGGCTGT
    GATGTTAACC TCAAGGAACA GGGGCAGCTG GTGCGACAGG ATGAGTTCAT GGTACGCACT
    GGGCGCCGTA AGTCTTTGCG CCGCGTTTTT CTTTTTGAGG AGCTGCTGCT CTTCAGCAAG
    CCCCGCCGAG GACCCACAGG CACCGACACA TTTACCTATA AGCGTTCCTT CAAGATGGCA
    GACCTGGGCC TCACTGAGTG CTGTGGGGAT AGCAACCTGC GGTTTGAGAT CTGGTTCCGC
    CGTCGCAAGG CTAGGGACAC CTTTGTGCTT CAGGCTGCCA GCTTGGCCAC TAAGCAGGCT
    TGGACGGCTG ACATTTCTCG TCTGCTCTGG AGGCAGGCCA TCCACAACAA GGAGGTGCGC
    ATGGCTGAGA TGGTGTCCAT GGGTGTGGGG AACAAGGCCT TCCGGGACCT CGCCCCCAGT
    GAGGAAGCTA TCAACGACCG CACTGTCGAC TATGTCCTGA AGTGCCGAGA AGTTCGCTCT
    CGGGCCTCTA TTGCTGTGGC CCCATTTGAC TGTGACAGCC GCTACCTGGG GGCCTGGAGT
    TCCCTTCCTG GAGACCCCGC CTCTTGCTCT GTACTGGGGT CCCTCAACCT GCACCTGTAC
    AGAGACCCGG CTCTTCTGGG AGTCCGCTGG CCCCTGTATT CTACCAGCGT CCCAGAGGAA
    GCAGCGCTGG AGGCCGAGGT GGAGCTGGGC AGTCAGCTGT CTTTGACTCC TGAGGACTCA
    GAGGTCTCAT CCCAGTGCCC ATCAGTCAGT GGATCCAGTG GCTCTGACAG CAGCTGTGTG
    TCAGGGCAGG TCCTGGGCAG GGGACTTGAG GACTTGTCCT GTATTCAAAT GCTGAAATTC
    TGCCCCAGTG GAAGAAACAA TAATGCCCTT GAACAACTCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe07253
    Clone ID Related Accession (Same CDS sequence) XM_022419321.1 , XM_014114094.2 , XM_005620823.2 , XM_014114094.1 , XM_005620823.3
    Accession Version XM_005620823.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3642bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product puratrophin-1 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726060.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005620823.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGAAGGGC CGCTGGAGGT TGGGGATGAG TCCCCAGACT CCCAGGGACA CGCCACTGAC 
    TGGAGGTTTG CTGTGTGCAG CTTCAGGGAT GCCTGGGATG AAGAGGAATC TGTTGCCCTC
    AAGCAGATTA AGGACTCCAA TCCTCCGGGT CCACCAGCAG GGACAGCCCA GGGGAGCCCC
    CTGCCTGAGG AACTTCAGTC ACCCCCAGGA CAGTTGGCTG TAGACGAGTC GGGAGATGGC
    CAGGGTTGCA CGTTAGAAGG TTCCTCACTC CGGTTAGATG AACCAATCCC CTCTGGAGTG
    GAGAGCCTCC TGTGCCCAAT GTCCTCCTGC CTCAGTCTTG AGCAGGGTGA GAGTGATAGT
    CAAGGGGGAG GCTTGGCAGG GGACCAACTT TCAAGCAGAT TAATGCCAGC CCACCTGGGT
    CCTGGGGGTT TGACCAGTGA TCCGGTGGAC CTTGGAGGCC CATCATCTGA GGTATCATCA
    GAGCTACTGG AGGCAGACCA CAGTGGCTCT AGCCTCCCTA AGCCTGCTGA TTACCTCCGG
    GCCCAAGACC TTGCCTGGGA TCTGCTGGCC AGTGGCATGG CTACCTTGCC AGGGACTCGC
    GATGTAGAAG GCCGGGCAGT GCTGCTTCTG TGTGCCCATA GCCCGGCCTG GCTTCACCTC
    AAGTGCACTA GCCATGAACT CCTCTGCCTC CTGCTCTACC TACGAGGCAT CCCCAGGCCT
    GAAGTACAGG CCCTGGGACT GACTGTTCTG GTGGATGCCC GACTTTGTTC CCCAAGCTCT
    TCCCTCCTCT GGGCGCTCAG CCAACTGCAA GAAGCAGCCC CAGGGTCAGT GTACCAGGTG
    CTGCTACTGG GAAAGATGCC GGAGGAAGTG CCTTCTGGGC TGCAGCTGCA GCAGCTGCCT
    TCTCATCAGA GCCTGCTGAA CCACGTCTCC ACCTCCGGGT TGCCGGCTTC AATGGGAGGA
    GGCCTGCCTT ATTCCCACCA GGCCTGGCTG GACTTCCGGA TGCGTCTGGA AGCCCTACTG
    CAAAGCTGCC AGGTGGCTTG TGCCCTGCTC CAGGGGGCCA TCGAGAGCAT GAAGGCTGTG
    CCCCAGCCTG TAGAGTCTGG GGAAGTTGGT CGGCTGCTAC AGCAGGCACG GGTCCTGATG
    GAGCACGTGC TAGACTCACC ACGGCTAGCA TGGCTACAAT GCCAAGGGGG TTTGGAGCTG
    GCATGGCTGA AGCAGGAAGT CCCAGAGGTG ACCCTGAGCC CAGACTACAG GTCAGCAGTG
    GATGAGGTTG ATGAACTCTA TGGCCGTGTA GATGGACTGC TGCACCAGTT GACCCTCCAG
    AGTAACCGGC GAGTACGGGC ACTAGAGTTG GTCCAGACTC TGGAGGCCCA GGAGGGTGGA
    CTACACCAGA TTGAAGTGTG GCTGCAGCAG GTGGGCTGGC CAGCCCTCGA GGAGCTAAAG
    GAGCCCTCAC TGGACATGCT GCTCCAGGCG AAAGGCCCTT TTCAGGAGCT GGACCAGGTT
    GCCCAGGAGC AGGTCAGGCG AGGGGAGAAG TTTCTACAGC CACTGGCTGG CTGGGAGGCT
    GCTGAACTGG GGCCCGCTGG GGCCCGCTTT CTGGCCCGGC AAGCCAAGCT GACTGACTTC
    TCCAGGGCTT TGACCCGGCG GCGGCAGCAG CTGGCAGATG CAGAGAGGCT ATTCCATCTT
    TTCAAGCAGG CCTCGACATG GGCTGAGGAG GGGCAGCGAG TGTTGGCAGA GCTGGAACAG
    GAGCACCCAG GGGTTGTGCT GCAACGGCTG CAGCTGCATT GGACCAAGCA CCCTGATTTG
    CCTCCTGCCC ACTTCCGAAA GATGTGGGCT CTGGCCACAG GGTTAGGTTC TGAGGGCATC
    CGCCAGGAGT GCCGCTGGGC CTGGGCACAA TGCCAGGACA CCTGGCTGGC ACTGGACCAG
    AAGCTAGAGG CTGTACTGAA GCCACCACTG ACGACGACCA TGACAGTCAG CACGGCTAGC
    CTGTGTGTCA GCAGAGTCCC AGCTGCATCT GCCACCCCTC CCCTGAGGAA GGCATACAGC
    CTGGATCAGA ATTTAGGGCA GAGTCTCAGA CAGCCTGCCC ACCACTGCCA CCACGTGGCC
    ACTGCGGCTG CCTGCCACGG ACCGGAGGCT GGAGGTGGTG CCCAGCCAGG GTCATCCCCT
    ACCATGCCTC TGCCAAGCAC CTCCGACCCC AGGAGCCCCA GCAGGCTCCA GCTGGTGCTG
    GCGGAGATGG TGGCTACGGA GCAGGAGTAT GTCCGTGCTC TTGACTACAC CATAGAGAAC
    TACTTCCCTG AGTTGGATCG TCCTGATGTG CCCCAGGGCC TCCGTGGGCA GCGTGCCCAC
    CTCTTTGGCA ACCTGGAGAA GCTGCGGGAC TTCCACTGTC ATTTCTTCCT GCGTGAACTG
    GAGGCCTGTA CCCAGCATCC ATCCCGGGTA GCCTATGCCT TCCTGCGCCA TAGGGTGCAG
    TTTGGGATGT ATGCACTCTA CAGCAAGAAT AAACCTCGCT CTGATGCCCT GATGACCAGC
    TTCGGTCATG CCTTCTTCAA GGACAAGCAG CAAGCACTGG GGGACCACCT GGACTTGGCC
    TCCTATCTGC TGAAGCCCAT CCAGCGCATG AGCAAGTATG CATTGCTGCT GCAGGAACTG
    GCACGGGCCT GTGGGGGTGC TGTGCAGGAG CTGAGTGCCC TGCGGGCTGC CCAGAGCCTT
    GTGCGCTTCC AGCTGCGACA CGGCAATGAC CTGCTAGCCA TGGATGCCAT TCAGGGCTGT
    GATGTTAACC TCAAGGAACA GGGGCAGCTG GTGCGACAGG ATGAGTTCAT GGTACGCACT
    GGGCGCCGTA AGTCTTTGCG CCGCGTTTTT CTTTTTGAGG AGCTGCTGCT CTTCAGCAAG
    CCCCGCCGAG GACCCACAGG CACCGACACA TTTACCTATA AGCGTTCCTT CAAGATGGCA
    GACCTGGGCC TCACTGAGTG CTGTGGGGAT AGCAACCTGC GGTTTGAGAT CTGGTTCCGC
    CGTCGCAAGG CTAGGGACAC CTTTGTGCTT CAGGCTGCCA GCTTGGCCAC TAAGCAGGCT
    TGGACGGCTG ACATTTCTCG TCTGCTCTGG AGGCAGGCCA TCCACAACAA GGAGGTGCGC
    ATGGCTGAGA TGGTGTCCAT GGGTGTGGGG AACAAGGCCT TCCGGGACCT CGCCCCCAGT
    GAGGAAGCTA TCAACGACCG CACTGTCGAC TATGTCCTGA AGTGCCGAGA AGTTCGCTCT
    CGGGCCTCTA TTGCTGTGGC CCCATTTGAC TGTGACAGCC GCTACCTGGG GGCCTGGAGT
    TCCCTTCCTG GAGACCCCGC CTCTTGCTCT GTACTGGGGT CCCTCAACCT GCACCTGTAC
    AGAGACCCGG CTCTTCTGGG AGTCCGCTGG CCCCTGTATT CTACCAGCGT CCCAGAGGAA
    GCAGCGCTGG AGGCCGAGGT GGAGCTGGGC AGTCAGCTGT CTTTGACTCC TGAGGACTCA
    GAGGTCTCAT CCCAGTGCCC ATCAGTCAGT GGATCCAGTG GCTCTGACAG CAGCTGTGTG
    TCAGGGCAGG TCCTGGGCAG GGGACTTGAG GACTTGTCCT GTATTCAAAT GCTGAAATTC
    TGCCCCAGTG GAAGAAACAA TAATGCCCTT GAACAACTCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005620880.1
    CDS415..4056
    Translation

    Target ORF information:

    RefSeq Version XM_005620823.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris pleckstrin homology domain containing, family G (with RhoGef domain) member 4 (PLEKHG4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005620823.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGAAGGGC CGCTGGAGGT TGGGGATGAG TCCCCAGACT CCCAGGGACA CGCCACTGAC 
    TGGAGGTTTG CTGTGTGCAG CTTCAGGGAT GCCTGGGATG AAGAGGAATC TGTTGCCCTC
    AAGCAGATTA AGGACTCCAA TCCTCCGGGT CCACCAGCAG GGACAGCCCA GGGGAGCCCC
    CTGCCTGAGG AACTTCAGTC ACCCCCAGGA CAGTTGGCTG TAGACGAGTC GGGAGATGGC
    CAGGGTTGCA CGTTAGAAGG TTCCTCACTC CGGTTAGATG AACCAATCCC CTCTGGAGTG
    GAGAGCCTCC TGTGCCCAAT GTCCTCCTGC CTCAGTCTTG AGCAGGGTGA GAGTGATAGT
    CAAGGGGGAG GCTTGGCAGG GGACCAACTT TCAAGCAGAT TAATGCCAGC CCACCTGGGT
    CCTGGGGGTT TGACCAGTGA TCCGGTGGAC CTTGGAGGCC CATCATCTGA GGTATCATCA
    GAGCTACTGG AGGCAGACCA CAGTGGCTCT AGCCTCCCTA AGCCTGCTGA TTACCTCCGG
    GCCCAAGACC TTGCCTGGGA TCTGCTGGCC AGTGGCATGG CTACCTTGCC AGGGACTCGC
    GATGTAGAAG GCCGGGCAGT GCTGCTTCTG TGTGCCCATA GCCCGGCCTG GCTTCACCTC
    AAGTGCACTA GCCATGAACT CCTCTGCCTC CTGCTCTACC TACGAGGCAT CCCCAGGCCT
    GAAGTACAGG CCCTGGGACT GACTGTTCTG GTGGATGCCC GACTTTGTTC CCCAAGCTCT
    TCCCTCCTCT GGGCGCTCAG CCAACTGCAA GAAGCAGCCC CAGGGTCAGT GTACCAGGTG
    CTGCTACTGG GAAAGATGCC GGAGGAAGTG CCTTCTGGGC TGCAGCTGCA GCAGCTGCCT
    TCTCATCAGA GCCTGCTGAA CCACGTCTCC ACCTCCGGGT TGCCGGCTTC AATGGGAGGA
    GGCCTGCCTT ATTCCCACCA GGCCTGGCTG GACTTCCGGA TGCGTCTGGA AGCCCTACTG
    CAAAGCTGCC AGGTGGCTTG TGCCCTGCTC CAGGGGGCCA TCGAGAGCAT GAAGGCTGTG
    CCCCAGCCTG TAGAGTCTGG GGAAGTTGGT CGGCTGCTAC AGCAGGCACG GGTCCTGATG
    GAGCACGTGC TAGACTCACC ACGGCTAGCA TGGCTACAAT GCCAAGGGGG TTTGGAGCTG
    GCATGGCTGA AGCAGGAAGT CCCAGAGGTG ACCCTGAGCC CAGACTACAG GTCAGCAGTG
    GATGAGGTTG ATGAACTCTA TGGCCGTGTA GATGGACTGC TGCACCAGTT GACCCTCCAG
    AGTAACCGGC GAGTACGGGC ACTAGAGTTG GTCCAGACTC TGGAGGCCCA GGAGGGTGGA
    CTACACCAGA TTGAAGTGTG GCTGCAGCAG GTGGGCTGGC CAGCCCTCGA GGAGCTAAAG
    GAGCCCTCAC TGGACATGCT GCTCCAGGCG AAAGGCCCTT TTCAGGAGCT GGACCAGGTT
    GCCCAGGAGC AGGTCAGGCG AGGGGAGAAG TTTCTACAGC CACTGGCTGG CTGGGAGGCT
    GCTGAACTGG GGCCCGCTGG GGCCCGCTTT CTGGCCCGGC AAGCCAAGCT GACTGACTTC
    TCCAGGGCTT TGACCCGGCG GCGGCAGCAG CTGGCAGATG CAGAGAGGCT ATTCCATCTT
    TTCAAGCAGG CCTCGACATG GGCTGAGGAG GGGCAGCGAG TGTTGGCAGA GCTGGAACAG
    GAGCACCCAG GGGTTGTGCT GCAACGGCTG CAGCTGCATT GGACCAAGCA CCCTGATTTG
    CCTCCTGCCC ACTTCCGAAA GATGTGGGCT CTGGCCACAG GGTTAGGTTC TGAGGGCATC
    CGCCAGGAGT GCCGCTGGGC CTGGGCACAA TGCCAGGACA CCTGGCTGGC ACTGGACCAG
    AAGCTAGAGG CTGTACTGAA GCCACCACTG ACGACGACCA TGACAGTCAG CACGGCTAGC
    CTGTGTGTCA GCAGAGTCCC AGCTGCATCT GCCACCCCTC CCCTGAGGAA GGCATACAGC
    CTGGATCAGA ATTTAGGGCA GAGTCTCAGA CAGCCTGCCC ACCACTGCCA CCACGTGGCC
    ACTGCGGCTG CCTGCCACGG ACCGGAGGCT GGAGGTGGTG CCCAGCCAGG GTCATCCCCT
    ACCATGCCTC TGCCAAGCAC CTCCGACCCC AGGAGCCCCA GCAGGCTCCA GCTGGTGCTG
    GCGGAGATGG TGGCTACGGA GCAGGAGTAT GTCCGTGCTC TTGACTACAC CATAGAGAAC
    TACTTCCCTG AGTTGGATCG TCCTGATGTG CCCCAGGGCC TCCGTGGGCA GCGTGCCCAC
    CTCTTTGGCA ACCTGGAGAA GCTGCGGGAC TTCCACTGTC ATTTCTTCCT GCGTGAACTG
    GAGGCCTGTA CCCAGCATCC ATCCCGGGTA GCCTATGCCT TCCTGCGCCA TAGGGTGCAG
    TTTGGGATGT ATGCACTCTA CAGCAAGAAT AAACCTCGCT CTGATGCCCT GATGACCAGC
    TTCGGTCATG CCTTCTTCAA GGACAAGCAG CAAGCACTGG GGGACCACCT GGACTTGGCC
    TCCTATCTGC TGAAGCCCAT CCAGCGCATG AGCAAGTATG CATTGCTGCT GCAGGAACTG
    GCACGGGCCT GTGGGGGTGC TGTGCAGGAG CTGAGTGCCC TGCGGGCTGC CCAGAGCCTT
    GTGCGCTTCC AGCTGCGACA CGGCAATGAC CTGCTAGCCA TGGATGCCAT TCAGGGCTGT
    GATGTTAACC TCAAGGAACA GGGGCAGCTG GTGCGACAGG ATGAGTTCAT GGTACGCACT
    GGGCGCCGTA AGTCTTTGCG CCGCGTTTTT CTTTTTGAGG AGCTGCTGCT CTTCAGCAAG
    CCCCGCCGAG GACCCACAGG CACCGACACA TTTACCTATA AGCGTTCCTT CAAGATGGCA
    GACCTGGGCC TCACTGAGTG CTGTGGGGAT AGCAACCTGC GGTTTGAGAT CTGGTTCCGC
    CGTCGCAAGG CTAGGGACAC CTTTGTGCTT CAGGCTGCCA GCTTGGCCAC TAAGCAGGCT
    TGGACGGCTG ACATTTCTCG TCTGCTCTGG AGGCAGGCCA TCCACAACAA GGAGGTGCGC
    ATGGCTGAGA TGGTGTCCAT GGGTGTGGGG AACAAGGCCT TCCGGGACCT CGCCCCCAGT
    GAGGAAGCTA TCAACGACCG CACTGTCGAC TATGTCCTGA AGTGCCGAGA AGTTCGCTCT
    CGGGCCTCTA TTGCTGTGGC CCCATTTGAC TGTGACAGCC GCTACCTGGG GGCCTGGAGT
    TCCCTTCCTG GAGACCCCGC CTCTTGCTCT GTACTGGGGT CCCTCAACCT GCACCTGTAC
    AGAGACCCGG CTCTTCTGGG AGTCCGCTGG CCCCTGTATT CTACCAGCGT CCCAGAGGAA
    GCAGCGCTGG AGGCCGAGGT GGAGCTGGGC AGTCAGCTGT CTTTGACTCC TGAGGACTCA
    GAGGTCTCAT CCCAGTGCCC ATCAGTCAGT GGATCCAGTG GCTCTGACAG CAGCTGTGTG
    TCAGGGCAGG TCCTGGGCAG GGGACTTGAG GACTTGTCCT GTATTCAAAT GCTGAAATTC
    TGCCCCAGTG GAAGAAACAA TAATGCCCTT GAACAACTCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe07253
    Clone ID Related Accession (Same CDS sequence) XM_022419321.1 , XM_014114094.2 , XM_005620823.2 , XM_014114094.1 , XM_005620823.3
    Accession Version XM_014114094.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3642bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product puratrophin-1 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726060.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGAAGGGC CGCTGGAGGT TGGGGATGAG TCCCCAGACT CCCAGGGACA CGCCACTGAC 
    TGGAGGTTTG CTGTGTGCAG CTTCAGGGAT GCCTGGGATG AAGAGGAATC TGTTGCCCTC
    AAGCAGATTA AGGACTCCAA TCCTCCGGGT CCACCAGCAG GGACAGCCCA GGGGAGCCCC
    CTGCCTGAGG AACTTCAGTC ACCCCCAGGA CAGTTGGCTG TAGACGAGTC GGGAGATGGC
    CAGGGTTGCA CGTTAGAAGG TTCCTCACTC CGGTTAGATG AACCAATCCC CTCTGGAGTG
    GAGAGCCTCC TGTGCCCAAT GTCCTCCTGC CTCAGTCTTG AGCAGGGTGA GAGTGATAGT
    CAAGGGGGAG GCTTGGCAGG GGACCAACTT TCAAGCAGAT TAATGCCAGC CCACCTGGGT
    CCTGGGGGTT TGACCAGTGA TCCGGTGGAC CTTGGAGGCC CATCATCTGA GGTATCATCA
    GAGCTACTGG AGGCAGACCA CAGTGGCTCT AGCCTCCCTA AGCCTGCTGA TTACCTCCGG
    GCCCAAGACC TTGCCTGGGA TCTGCTGGCC AGTGGCATGG CTACCTTGCC AGGGACTCGC
    GATGTAGAAG GCCGGGCAGT GCTGCTTCTG TGTGCCCATA GCCCGGCCTG GCTTCACCTC
    AAGTGCACTA GCCATGAACT CCTCTGCCTC CTGCTCTACC TACGAGGCAT CCCCAGGCCT
    GAAGTACAGG CCCTGGGACT GACTGTTCTG GTGGATGCCC GACTTTGTTC CCCAAGCTCT
    TCCCTCCTCT GGGCGCTCAG CCAACTGCAA GAAGCAGCCC CAGGGTCAGT GTACCAGGTG
    CTGCTACTGG GAAAGATGCC GGAGGAAGTG CCTTCTGGGC TGCAGCTGCA GCAGCTGCCT
    TCTCATCAGA GCCTGCTGAA CCACGTCTCC ACCTCCGGGT TGCCGGCTTC AATGGGAGGA
    GGCCTGCCTT ATTCCCACCA GGCCTGGCTG GACTTCCGGA TGCGTCTGGA AGCCCTACTG
    CAAAGCTGCC AGGTGGCTTG TGCCCTGCTC CAGGGGGCCA TCGAGAGCAT GAAGGCTGTG
    CCCCAGCCTG TAGAGTCTGG GGAAGTTGGT CGGCTGCTAC AGCAGGCACG GGTCCTGATG
    GAGCACGTGC TAGACTCACC ACGGCTAGCA TGGCTACAAT GCCAAGGGGG TTTGGAGCTG
    GCATGGCTGA AGCAGGAAGT CCCAGAGGTG ACCCTGAGCC CAGACTACAG GTCAGCAGTG
    GATGAGGTTG ATGAACTCTA TGGCCGTGTA GATGGACTGC TGCACCAGTT GACCCTCCAG
    AGTAACCGGC GAGTACGGGC ACTAGAGTTG GTCCAGACTC TGGAGGCCCA GGAGGGTGGA
    CTACACCAGA TTGAAGTGTG GCTGCAGCAG GTGGGCTGGC CAGCCCTCGA GGAGCTAAAG
    GAGCCCTCAC TGGACATGCT GCTCCAGGCG AAAGGCCCTT TTCAGGAGCT GGACCAGGTT
    GCCCAGGAGC AGGTCAGGCG AGGGGAGAAG TTTCTACAGC CACTGGCTGG CTGGGAGGCT
    GCTGAACTGG GGCCCGCTGG GGCCCGCTTT CTGGCCCGGC AAGCCAAGCT GACTGACTTC
    TCCAGGGCTT TGACCCGGCG GCGGCAGCAG CTGGCAGATG CAGAGAGGCT ATTCCATCTT
    TTCAAGCAGG CCTCGACATG GGCTGAGGAG GGGCAGCGAG TGTTGGCAGA GCTGGAACAG
    GAGCACCCAG GGGTTGTGCT GCAACGGCTG CAGCTGCATT GGACCAAGCA CCCTGATTTG
    CCTCCTGCCC ACTTCCGAAA GATGTGGGCT CTGGCCACAG GGTTAGGTTC TGAGGGCATC
    CGCCAGGAGT GCCGCTGGGC CTGGGCACAA TGCCAGGACA CCTGGCTGGC ACTGGACCAG
    AAGCTAGAGG CTGTACTGAA GCCACCACTG ACGACGACCA TGACAGTCAG CACGGCTAGC
    CTGTGTGTCA GCAGAGTCCC AGCTGCATCT GCCACCCCTC CCCTGAGGAA GGCATACAGC
    CTGGATCAGA ATTTAGGGCA GAGTCTCAGA CAGCCTGCCC ACCACTGCCA CCACGTGGCC
    ACTGCGGCTG CCTGCCACGG ACCGGAGGCT GGAGGTGGTG CCCAGCCAGG GTCATCCCCT
    ACCATGCCTC TGCCAAGCAC CTCCGACCCC AGGAGCCCCA GCAGGCTCCA GCTGGTGCTG
    GCGGAGATGG TGGCTACGGA GCAGGAGTAT GTCCGTGCTC TTGACTACAC CATAGAGAAC
    TACTTCCCTG AGTTGGATCG TCCTGATGTG CCCCAGGGCC TCCGTGGGCA GCGTGCCCAC
    CTCTTTGGCA ACCTGGAGAA GCTGCGGGAC TTCCACTGTC ATTTCTTCCT GCGTGAACTG
    GAGGCCTGTA CCCAGCATCC ATCCCGGGTA GCCTATGCCT TCCTGCGCCA TAGGGTGCAG
    TTTGGGATGT ATGCACTCTA CAGCAAGAAT AAACCTCGCT CTGATGCCCT GATGACCAGC
    TTCGGTCATG CCTTCTTCAA GGACAAGCAG CAAGCACTGG GGGACCACCT GGACTTGGCC
    TCCTATCTGC TGAAGCCCAT CCAGCGCATG AGCAAGTATG CATTGCTGCT GCAGGAACTG
    GCACGGGCCT GTGGGGGTGC TGTGCAGGAG CTGAGTGCCC TGCGGGCTGC CCAGAGCCTT
    GTGCGCTTCC AGCTGCGACA CGGCAATGAC CTGCTAGCCA TGGATGCCAT TCAGGGCTGT
    GATGTTAACC TCAAGGAACA GGGGCAGCTG GTGCGACAGG ATGAGTTCAT GGTACGCACT
    GGGCGCCGTA AGTCTTTGCG CCGCGTTTTT CTTTTTGAGG AGCTGCTGCT CTTCAGCAAG
    CCCCGCCGAG GACCCACAGG CACCGACACA TTTACCTATA AGCGTTCCTT CAAGATGGCA
    GACCTGGGCC TCACTGAGTG CTGTGGGGAT AGCAACCTGC GGTTTGAGAT CTGGTTCCGC
    CGTCGCAAGG CTAGGGACAC CTTTGTGCTT CAGGCTGCCA GCTTGGCCAC TAAGCAGGCT
    TGGACGGCTG ACATTTCTCG TCTGCTCTGG AGGCAGGCCA TCCACAACAA GGAGGTGCGC
    ATGGCTGAGA TGGTGTCCAT GGGTGTGGGG AACAAGGCCT TCCGGGACCT CGCCCCCAGT
    GAGGAAGCTA TCAACGACCG CACTGTCGAC TATGTCCTGA AGTGCCGAGA AGTTCGCTCT
    CGGGCCTCTA TTGCTGTGGC CCCATTTGAC TGTGACAGCC GCTACCTGGG GGCCTGGAGT
    TCCCTTCCTG GAGACCCCGC CTCTTGCTCT GTACTGGGGT CCCTCAACCT GCACCTGTAC
    AGAGACCCGG CTCTTCTGGG AGTCCGCTGG CCCCTGTATT CTACCAGCGT CCCAGAGGAA
    GCAGCGCTGG AGGCCGAGGT GGAGCTGGGC AGTCAGCTGT CTTTGACTCC TGAGGACTCA
    GAGGTCTCAT CCCAGTGCCC ATCAGTCAGT GGATCCAGTG GCTCTGACAG CAGCTGTGTG
    TCAGGGCAGG TCCTGGGCAG GGGACTTGAG GACTTGTCCT GTATTCAAAT GCTGAAATTC
    TGCCCCAGTG GAAGAAACAA TAATGCCCTT GAACAACTCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013969569.1
    CDS375..4016
    Translation

    Target ORF information:

    RefSeq Version XM_014114094.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris pleckstrin homology domain containing, family G (with RhoGef domain) member 4 (PLEKHG4), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014114094.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGAAGGGC CGCTGGAGGT TGGGGATGAG TCCCCAGACT CCCAGGGACA CGCCACTGAC 
    TGGAGGTTTG CTGTGTGCAG CTTCAGGGAT GCCTGGGATG AAGAGGAATC TGTTGCCCTC
    AAGCAGATTA AGGACTCCAA TCCTCCGGGT CCACCAGCAG GGACAGCCCA GGGGAGCCCC
    CTGCCTGAGG AACTTCAGTC ACCCCCAGGA CAGTTGGCTG TAGACGAGTC GGGAGATGGC
    CAGGGTTGCA CGTTAGAAGG TTCCTCACTC CGGTTAGATG AACCAATCCC CTCTGGAGTG
    GAGAGCCTCC TGTGCCCAAT GTCCTCCTGC CTCAGTCTTG AGCAGGGTGA GAGTGATAGT
    CAAGGGGGAG GCTTGGCAGG GGACCAACTT TCAAGCAGAT TAATGCCAGC CCACCTGGGT
    CCTGGGGGTT TGACCAGTGA TCCGGTGGAC CTTGGAGGCC CATCATCTGA GGTATCATCA
    GAGCTACTGG AGGCAGACCA CAGTGGCTCT AGCCTCCCTA AGCCTGCTGA TTACCTCCGG
    GCCCAAGACC TTGCCTGGGA TCTGCTGGCC AGTGGCATGG CTACCTTGCC AGGGACTCGC
    GATGTAGAAG GCCGGGCAGT GCTGCTTCTG TGTGCCCATA GCCCGGCCTG GCTTCACCTC
    AAGTGCACTA GCCATGAACT CCTCTGCCTC CTGCTCTACC TACGAGGCAT CCCCAGGCCT
    GAAGTACAGG CCCTGGGACT GACTGTTCTG GTGGATGCCC GACTTTGTTC CCCAAGCTCT
    TCCCTCCTCT GGGCGCTCAG CCAACTGCAA GAAGCAGCCC CAGGGTCAGT GTACCAGGTG
    CTGCTACTGG GAAAGATGCC GGAGGAAGTG CCTTCTGGGC TGCAGCTGCA GCAGCTGCCT
    TCTCATCAGA GCCTGCTGAA CCACGTCTCC ACCTCCGGGT TGCCGGCTTC AATGGGAGGA
    GGCCTGCCTT ATTCCCACCA GGCCTGGCTG GACTTCCGGA TGCGTCTGGA AGCCCTACTG
    CAAAGCTGCC AGGTGGCTTG TGCCCTGCTC CAGGGGGCCA TCGAGAGCAT GAAGGCTGTG
    CCCCAGCCTG TAGAGTCTGG GGAAGTTGGT CGGCTGCTAC AGCAGGCACG GGTCCTGATG
    GAGCACGTGC TAGACTCACC ACGGCTAGCA TGGCTACAAT GCCAAGGGGG TTTGGAGCTG
    GCATGGCTGA AGCAGGAAGT CCCAGAGGTG ACCCTGAGCC CAGACTACAG GTCAGCAGTG
    GATGAGGTTG ATGAACTCTA TGGCCGTGTA GATGGACTGC TGCACCAGTT GACCCTCCAG
    AGTAACCGGC GAGTACGGGC ACTAGAGTTG GTCCAGACTC TGGAGGCCCA GGAGGGTGGA
    CTACACCAGA TTGAAGTGTG GCTGCAGCAG GTGGGCTGGC CAGCCCTCGA GGAGCTAAAG
    GAGCCCTCAC TGGACATGCT GCTCCAGGCG AAAGGCCCTT TTCAGGAGCT GGACCAGGTT
    GCCCAGGAGC AGGTCAGGCG AGGGGAGAAG TTTCTACAGC CACTGGCTGG CTGGGAGGCT
    GCTGAACTGG GGCCCGCTGG GGCCCGCTTT CTGGCCCGGC AAGCCAAGCT GACTGACTTC
    TCCAGGGCTT TGACCCGGCG GCGGCAGCAG CTGGCAGATG CAGAGAGGCT ATTCCATCTT
    TTCAAGCAGG CCTCGACATG GGCTGAGGAG GGGCAGCGAG TGTTGGCAGA GCTGGAACAG
    GAGCACCCAG GGGTTGTGCT GCAACGGCTG CAGCTGCATT GGACCAAGCA CCCTGATTTG
    CCTCCTGCCC ACTTCCGAAA GATGTGGGCT CTGGCCACAG GGTTAGGTTC TGAGGGCATC
    CGCCAGGAGT GCCGCTGGGC CTGGGCACAA TGCCAGGACA CCTGGCTGGC ACTGGACCAG
    AAGCTAGAGG CTGTACTGAA GCCACCACTG ACGACGACCA TGACAGTCAG CACGGCTAGC
    CTGTGTGTCA GCAGAGTCCC AGCTGCATCT GCCACCCCTC CCCTGAGGAA GGCATACAGC
    CTGGATCAGA ATTTAGGGCA GAGTCTCAGA CAGCCTGCCC ACCACTGCCA CCACGTGGCC
    ACTGCGGCTG CCTGCCACGG ACCGGAGGCT GGAGGTGGTG CCCAGCCAGG GTCATCCCCT
    ACCATGCCTC TGCCAAGCAC CTCCGACCCC AGGAGCCCCA GCAGGCTCCA GCTGGTGCTG
    GCGGAGATGG TGGCTACGGA GCAGGAGTAT GTCCGTGCTC TTGACTACAC CATAGAGAAC
    TACTTCCCTG AGTTGGATCG TCCTGATGTG CCCCAGGGCC TCCGTGGGCA GCGTGCCCAC
    CTCTTTGGCA ACCTGGAGAA GCTGCGGGAC TTCCACTGTC ATTTCTTCCT GCGTGAACTG
    GAGGCCTGTA CCCAGCATCC ATCCCGGGTA GCCTATGCCT TCCTGCGCCA TAGGGTGCAG
    TTTGGGATGT ATGCACTCTA CAGCAAGAAT AAACCTCGCT CTGATGCCCT GATGACCAGC
    TTCGGTCATG CCTTCTTCAA GGACAAGCAG CAAGCACTGG GGGACCACCT GGACTTGGCC
    TCCTATCTGC TGAAGCCCAT CCAGCGCATG AGCAAGTATG CATTGCTGCT GCAGGAACTG
    GCACGGGCCT GTGGGGGTGC TGTGCAGGAG CTGAGTGCCC TGCGGGCTGC CCAGAGCCTT
    GTGCGCTTCC AGCTGCGACA CGGCAATGAC CTGCTAGCCA TGGATGCCAT TCAGGGCTGT
    GATGTTAACC TCAAGGAACA GGGGCAGCTG GTGCGACAGG ATGAGTTCAT GGTACGCACT
    GGGCGCCGTA AGTCTTTGCG CCGCGTTTTT CTTTTTGAGG AGCTGCTGCT CTTCAGCAAG
    CCCCGCCGAG GACCCACAGG CACCGACACA TTTACCTATA AGCGTTCCTT CAAGATGGCA
    GACCTGGGCC TCACTGAGTG CTGTGGGGAT AGCAACCTGC GGTTTGAGAT CTGGTTCCGC
    CGTCGCAAGG CTAGGGACAC CTTTGTGCTT CAGGCTGCCA GCTTGGCCAC TAAGCAGGCT
    TGGACGGCTG ACATTTCTCG TCTGCTCTGG AGGCAGGCCA TCCACAACAA GGAGGTGCGC
    ATGGCTGAGA TGGTGTCCAT GGGTGTGGGG AACAAGGCCT TCCGGGACCT CGCCCCCAGT
    GAGGAAGCTA TCAACGACCG CACTGTCGAC TATGTCCTGA AGTGCCGAGA AGTTCGCTCT
    CGGGCCTCTA TTGCTGTGGC CCCATTTGAC TGTGACAGCC GCTACCTGGG GGCCTGGAGT
    TCCCTTCCTG GAGACCCCGC CTCTTGCTCT GTACTGGGGT CCCTCAACCT GCACCTGTAC
    AGAGACCCGG CTCTTCTGGG AGTCCGCTGG CCCCTGTATT CTACCAGCGT CCCAGAGGAA
    GCAGCGCTGG AGGCCGAGGT GGAGCTGGGC AGTCAGCTGT CTTTGACTCC TGAGGACTCA
    GAGGTCTCAT CCCAGTGCCC ATCAGTCAGT GGATCCAGTG GCTCTGACAG CAGCTGTGTG
    TCAGGGCAGG TCCTGGGCAG GGGACTTGAG GACTTGTCCT GTATTCAAAT GCTGAAATTC
    TGCCCCAGTG GAAGAAACAA TAATGCCCTT GAACAACTCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe07253
    Clone ID Related Accession (Same CDS sequence) XM_022419321.1 , XM_014114094.2 , XM_005620823.2 , XM_014114094.1 , XM_005620823.3
    Accession Version XM_005620823.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3642bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product puratrophin-1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005620823.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGAAGGGC CGCTGGAGGT TGGGGATGAG TCCCCAGACT CCCAGGGACA CGCCACTGAC 
    TGGAGGTTTG CTGTGTGCAG CTTCAGGGAT GCCTGGGATG AAGAGGAATC TGTTGCCCTC
    AAGCAGATTA AGGACTCCAA TCCTCCGGGT CCACCAGCAG GGACAGCCCA GGGGAGCCCC
    CTGCCTGAGG AACTTCAGTC ACCCCCAGGA CAGTTGGCTG TAGACGAGTC GGGAGATGGC
    CAGGGTTGCA CGTTAGAAGG TTCCTCACTC CGGTTAGATG AACCAATCCC CTCTGGAGTG
    GAGAGCCTCC TGTGCCCAAT GTCCTCCTGC CTCAGTCTTG AGCAGGGTGA GAGTGATAGT
    CAAGGGGGAG GCTTGGCAGG GGACCAACTT TCAAGCAGAT TAATGCCAGC CCACCTGGGT
    CCTGGGGGTT TGACCAGTGA TCCGGTGGAC CTTGGAGGCC CATCATCTGA GGTATCATCA
    GAGCTACTGG AGGCAGACCA CAGTGGCTCT AGCCTCCCTA AGCCTGCTGA TTACCTCCGG
    GCCCAAGACC TTGCCTGGGA TCTGCTGGCC AGTGGCATGG CTACCTTGCC AGGGACTCGC
    GATGTAGAAG GCCGGGCAGT GCTGCTTCTG TGTGCCCATA GCCCGGCCTG GCTTCACCTC
    AAGTGCACTA GCCATGAACT CCTCTGCCTC CTGCTCTACC TACGAGGCAT CCCCAGGCCT
    GAAGTACAGG CCCTGGGACT GACTGTTCTG GTGGATGCCC GACTTTGTTC CCCAAGCTCT
    TCCCTCCTCT GGGCGCTCAG CCAACTGCAA GAAGCAGCCC CAGGGTCAGT GTACCAGGTG
    CTGCTACTGG GAAAGATGCC GGAGGAAGTG CCTTCTGGGC TGCAGCTGCA GCAGCTGCCT
    TCTCATCAGA GCCTGCTGAA CCACGTCTCC ACCTCCGGGT TGCCGGCTTC AATGGGAGGA
    GGCCTGCCTT ATTCCCACCA GGCCTGGCTG GACTTCCGGA TGCGTCTGGA AGCCCTACTG
    CAAAGCTGCC AGGTGGCTTG TGCCCTGCTC CAGGGGGCCA TCGAGAGCAT GAAGGCTGTG
    CCCCAGCCTG TAGAGTCTGG GGAAGTTGGT CGGCTGCTAC AGCAGGCACG GGTCCTGATG
    GAGCACGTGC TAGACTCACC ACGGCTAGCA TGGCTACAAT GCCAAGGGGG TTTGGAGCTG
    GCATGGCTGA AGCAGGAAGT CCCAGAGGTG ACCCTGAGCC CAGACTACAG GTCAGCAGTG
    GATGAGGTTG ATGAACTCTA TGGCCGTGTA GATGGACTGC TGCACCAGTT GACCCTCCAG
    AGTAACCGGC GAGTACGGGC ACTAGAGTTG GTCCAGACTC TGGAGGCCCA GGAGGGTGGA
    CTACACCAGA TTGAAGTGTG GCTGCAGCAG GTGGGCTGGC CAGCCCTCGA GGAGCTAAAG
    GAGCCCTCAC TGGACATGCT GCTCCAGGCG AAAGGCCCTT TTCAGGAGCT GGACCAGGTT
    GCCCAGGAGC AGGTCAGGCG AGGGGAGAAG TTTCTACAGC CACTGGCTGG CTGGGAGGCT
    GCTGAACTGG GGCCCGCTGG GGCCCGCTTT CTGGCCCGGC AAGCCAAGCT GACTGACTTC
    TCCAGGGCTT TGACCCGGCG GCGGCAGCAG CTGGCAGATG CAGAGAGGCT ATTCCATCTT
    TTCAAGCAGG CCTCGACATG GGCTGAGGAG GGGCAGCGAG TGTTGGCAGA GCTGGAACAG
    GAGCACCCAG GGGTTGTGCT GCAACGGCTG CAGCTGCATT GGACCAAGCA CCCTGATTTG
    CCTCCTGCCC ACTTCCGAAA GATGTGGGCT CTGGCCACAG GGTTAGGTTC TGAGGGCATC
    CGCCAGGAGT GCCGCTGGGC CTGGGCACAA TGCCAGGACA CCTGGCTGGC ACTGGACCAG
    AAGCTAGAGG CTGTACTGAA GCCACCACTG ACGACGACCA TGACAGTCAG CACGGCTAGC
    CTGTGTGTCA GCAGAGTCCC AGCTGCATCT GCCACCCCTC CCCTGAGGAA GGCATACAGC
    CTGGATCAGA ATTTAGGGCA GAGTCTCAGA CAGCCTGCCC ACCACTGCCA CCACGTGGCC
    ACTGCGGCTG CCTGCCACGG ACCGGAGGCT GGAGGTGGTG CCCAGCCAGG GTCATCCCCT
    ACCATGCCTC TGCCAAGCAC CTCCGACCCC AGGAGCCCCA GCAGGCTCCA GCTGGTGCTG
    GCGGAGATGG TGGCTACGGA GCAGGAGTAT GTCCGTGCTC TTGACTACAC CATAGAGAAC
    TACTTCCCTG AGTTGGATCG TCCTGATGTG CCCCAGGGCC TCCGTGGGCA GCGTGCCCAC
    CTCTTTGGCA ACCTGGAGAA GCTGCGGGAC TTCCACTGTC ATTTCTTCCT GCGTGAACTG
    GAGGCCTGTA CCCAGCATCC ATCCCGGGTA GCCTATGCCT TCCTGCGCCA TAGGGTGCAG
    TTTGGGATGT ATGCACTCTA CAGCAAGAAT AAACCTCGCT CTGATGCCCT GATGACCAGC
    TTCGGTCATG CCTTCTTCAA GGACAAGCAG CAAGCACTGG GGGACCACCT GGACTTGGCC
    TCCTATCTGC TGAAGCCCAT CCAGCGCATG AGCAAGTATG CATTGCTGCT GCAGGAACTG
    GCACGGGCCT GTGGGGGTGC TGTGCAGGAG CTGAGTGCCC TGCGGGCTGC CCAGAGCCTT
    GTGCGCTTCC AGCTGCGACA CGGCAATGAC CTGCTAGCCA TGGATGCCAT TCAGGGCTGT
    GATGTTAACC TCAAGGAACA GGGGCAGCTG GTGCGACAGG ATGAGTTCAT GGTACGCACT
    GGGCGCCGTA AGTCTTTGCG CCGCGTTTTT CTTTTTGAGG AGCTGCTGCT CTTCAGCAAG
    CCCCGCCGAG GACCCACAGG CACCGACACA TTTACCTATA AGCGTTCCTT CAAGATGGCA
    GACCTGGGCC TCACTGAGTG CTGTGGGGAT AGCAACCTGC GGTTTGAGAT CTGGTTCCGC
    CGTCGCAAGG CTAGGGACAC CTTTGTGCTT CAGGCTGCCA GCTTGGCCAC TAAGCAGGCT
    TGGACGGCTG ACATTTCTCG TCTGCTCTGG AGGCAGGCCA TCCACAACAA GGAGGTGCGC
    ATGGCTGAGA TGGTGTCCAT GGGTGTGGGG AACAAGGCCT TCCGGGACCT CGCCCCCAGT
    GAGGAAGCTA TCAACGACCG CACTGTCGAC TATGTCCTGA AGTGCCGAGA AGTTCGCTCT
    CGGGCCTCTA TTGCTGTGGC CCCATTTGAC TGTGACAGCC GCTACCTGGG GGCCTGGAGT
    TCCCTTCCTG GAGACCCCGC CTCTTGCTCT GTACTGGGGT CCCTCAACCT GCACCTGTAC
    AGAGACCCGG CTCTTCTGGG AGTCCGCTGG CCCCTGTATT CTACCAGCGT CCCAGAGGAA
    GCAGCGCTGG AGGCCGAGGT GGAGCTGGGC AGTCAGCTGT CTTTGACTCC TGAGGACTCA
    GAGGTCTCAT CCCAGTGCCC ATCAGTCAGT GGATCCAGTG GCTCTGACAG CAGCTGTGTG
    TCAGGGCAGG TCCTGGGCAG GGGACTTGAG GACTTGTCCT GTATTCAAAT GCTGAAATTC
    TGCCCCAGTG GAAGAAACAA TAATGCCCTT GAACAACTCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005620880.1
    CDS287..3928
    Translation

    Target ORF information:

    RefSeq Version XM_005620823.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris pleckstrin homology and RhoGEF domain containing G4 (PLEKHG4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005620823.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGAAGGGC CGCTGGAGGT TGGGGATGAG TCCCCAGACT CCCAGGGACA CGCCACTGAC 
    TGGAGGTTTG CTGTGTGCAG CTTCAGGGAT GCCTGGGATG AAGAGGAATC TGTTGCCCTC
    AAGCAGATTA AGGACTCCAA TCCTCCGGGT CCACCAGCAG GGACAGCCCA GGGGAGCCCC
    CTGCCTGAGG AACTTCAGTC ACCCCCAGGA CAGTTGGCTG TAGACGAGTC GGGAGATGGC
    CAGGGTTGCA CGTTAGAAGG TTCCTCACTC CGGTTAGATG AACCAATCCC CTCTGGAGTG
    GAGAGCCTCC TGTGCCCAAT GTCCTCCTGC CTCAGTCTTG AGCAGGGTGA GAGTGATAGT
    CAAGGGGGAG GCTTGGCAGG GGACCAACTT TCAAGCAGAT TAATGCCAGC CCACCTGGGT
    CCTGGGGGTT TGACCAGTGA TCCGGTGGAC CTTGGAGGCC CATCATCTGA GGTATCATCA
    GAGCTACTGG AGGCAGACCA CAGTGGCTCT AGCCTCCCTA AGCCTGCTGA TTACCTCCGG
    GCCCAAGACC TTGCCTGGGA TCTGCTGGCC AGTGGCATGG CTACCTTGCC AGGGACTCGC
    GATGTAGAAG GCCGGGCAGT GCTGCTTCTG TGTGCCCATA GCCCGGCCTG GCTTCACCTC
    AAGTGCACTA GCCATGAACT CCTCTGCCTC CTGCTCTACC TACGAGGCAT CCCCAGGCCT
    GAAGTACAGG CCCTGGGACT GACTGTTCTG GTGGATGCCC GACTTTGTTC CCCAAGCTCT
    TCCCTCCTCT GGGCGCTCAG CCAACTGCAA GAAGCAGCCC CAGGGTCAGT GTACCAGGTG
    CTGCTACTGG GAAAGATGCC GGAGGAAGTG CCTTCTGGGC TGCAGCTGCA GCAGCTGCCT
    TCTCATCAGA GCCTGCTGAA CCACGTCTCC ACCTCCGGGT TGCCGGCTTC AATGGGAGGA
    GGCCTGCCTT ATTCCCACCA GGCCTGGCTG GACTTCCGGA TGCGTCTGGA AGCCCTACTG
    CAAAGCTGCC AGGTGGCTTG TGCCCTGCTC CAGGGGGCCA TCGAGAGCAT GAAGGCTGTG
    CCCCAGCCTG TAGAGTCTGG GGAAGTTGGT CGGCTGCTAC AGCAGGCACG GGTCCTGATG
    GAGCACGTGC TAGACTCACC ACGGCTAGCA TGGCTACAAT GCCAAGGGGG TTTGGAGCTG
    GCATGGCTGA AGCAGGAAGT CCCAGAGGTG ACCCTGAGCC CAGACTACAG GTCAGCAGTG
    GATGAGGTTG ATGAACTCTA TGGCCGTGTA GATGGACTGC TGCACCAGTT GACCCTCCAG
    AGTAACCGGC GAGTACGGGC ACTAGAGTTG GTCCAGACTC TGGAGGCCCA GGAGGGTGGA
    CTACACCAGA TTGAAGTGTG GCTGCAGCAG GTGGGCTGGC CAGCCCTCGA GGAGCTAAAG
    GAGCCCTCAC TGGACATGCT GCTCCAGGCG AAAGGCCCTT TTCAGGAGCT GGACCAGGTT
    GCCCAGGAGC AGGTCAGGCG AGGGGAGAAG TTTCTACAGC CACTGGCTGG CTGGGAGGCT
    GCTGAACTGG GGCCCGCTGG GGCCCGCTTT CTGGCCCGGC AAGCCAAGCT GACTGACTTC
    TCCAGGGCTT TGACCCGGCG GCGGCAGCAG CTGGCAGATG CAGAGAGGCT ATTCCATCTT
    TTCAAGCAGG CCTCGACATG GGCTGAGGAG GGGCAGCGAG TGTTGGCAGA GCTGGAACAG
    GAGCACCCAG GGGTTGTGCT GCAACGGCTG CAGCTGCATT GGACCAAGCA CCCTGATTTG
    CCTCCTGCCC ACTTCCGAAA GATGTGGGCT CTGGCCACAG GGTTAGGTTC TGAGGGCATC
    CGCCAGGAGT GCCGCTGGGC CTGGGCACAA TGCCAGGACA CCTGGCTGGC ACTGGACCAG
    AAGCTAGAGG CTGTACTGAA GCCACCACTG ACGACGACCA TGACAGTCAG CACGGCTAGC
    CTGTGTGTCA GCAGAGTCCC AGCTGCATCT GCCACCCCTC CCCTGAGGAA GGCATACAGC
    CTGGATCAGA ATTTAGGGCA GAGTCTCAGA CAGCCTGCCC ACCACTGCCA CCACGTGGCC
    ACTGCGGCTG CCTGCCACGG ACCGGAGGCT GGAGGTGGTG CCCAGCCAGG GTCATCCCCT
    ACCATGCCTC TGCCAAGCAC CTCCGACCCC AGGAGCCCCA GCAGGCTCCA GCTGGTGCTG
    GCGGAGATGG TGGCTACGGA GCAGGAGTAT GTCCGTGCTC TTGACTACAC CATAGAGAAC
    TACTTCCCTG AGTTGGATCG TCCTGATGTG CCCCAGGGCC TCCGTGGGCA GCGTGCCCAC
    CTCTTTGGCA ACCTGGAGAA GCTGCGGGAC TTCCACTGTC ATTTCTTCCT GCGTGAACTG
    GAGGCCTGTA CCCAGCATCC ATCCCGGGTA GCCTATGCCT TCCTGCGCCA TAGGGTGCAG
    TTTGGGATGT ATGCACTCTA CAGCAAGAAT AAACCTCGCT CTGATGCCCT GATGACCAGC
    TTCGGTCATG CCTTCTTCAA GGACAAGCAG CAAGCACTGG GGGACCACCT GGACTTGGCC
    TCCTATCTGC TGAAGCCCAT CCAGCGCATG AGCAAGTATG CATTGCTGCT GCAGGAACTG
    GCACGGGCCT GTGGGGGTGC TGTGCAGGAG CTGAGTGCCC TGCGGGCTGC CCAGAGCCTT
    GTGCGCTTCC AGCTGCGACA CGGCAATGAC CTGCTAGCCA TGGATGCCAT TCAGGGCTGT
    GATGTTAACC TCAAGGAACA GGGGCAGCTG GTGCGACAGG ATGAGTTCAT GGTACGCACT
    GGGCGCCGTA AGTCTTTGCG CCGCGTTTTT CTTTTTGAGG AGCTGCTGCT CTTCAGCAAG
    CCCCGCCGAG GACCCACAGG CACCGACACA TTTACCTATA AGCGTTCCTT CAAGATGGCA
    GACCTGGGCC TCACTGAGTG CTGTGGGGAT AGCAACCTGC GGTTTGAGAT CTGGTTCCGC
    CGTCGCAAGG CTAGGGACAC CTTTGTGCTT CAGGCTGCCA GCTTGGCCAC TAAGCAGGCT
    TGGACGGCTG ACATTTCTCG TCTGCTCTGG AGGCAGGCCA TCCACAACAA GGAGGTGCGC
    ATGGCTGAGA TGGTGTCCAT GGGTGTGGGG AACAAGGCCT TCCGGGACCT CGCCCCCAGT
    GAGGAAGCTA TCAACGACCG CACTGTCGAC TATGTCCTGA AGTGCCGAGA AGTTCGCTCT
    CGGGCCTCTA TTGCTGTGGC CCCATTTGAC TGTGACAGCC GCTACCTGGG GGCCTGGAGT
    TCCCTTCCTG GAGACCCCGC CTCTTGCTCT GTACTGGGGT CCCTCAACCT GCACCTGTAC
    AGAGACCCGG CTCTTCTGGG AGTCCGCTGG CCCCTGTATT CTACCAGCGT CCCAGAGGAA
    GCAGCGCTGG AGGCCGAGGT GGAGCTGGGC AGTCAGCTGT CTTTGACTCC TGAGGACTCA
    GAGGTCTCAT CCCAGTGCCC ATCAGTCAGT GGATCCAGTG GCTCTGACAG CAGCTGTGTG
    TCAGGGCAGG TCCTGGGCAG GGGACTTGAG GACTTGTCCT GTATTCAAAT GCTGAAATTC
    TGCCCCAGTG GAAGAAACAA TAATGCCCTT GAACAACTCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.