LOC479708 cDNA ORF clone, Canis lupus familiaris(dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC479708
Entrez Gene ID	479708
Full Name	S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase
Synonyms	TYW1
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_022419526.1	XP_022275234.1	S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase isoform X2
XM_022419527.1	XP_022275235.1	S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase isoform X3
XM_005620930.2	XP_005620987.1	S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase
XM_005620930.3	XP_005620987.1	S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	TYW1	NP_060734.2
Mus musculus (house mouse)	Tyw1	NP_001015876.1
Schizosaccharomyces pombe (fission yeast)	SPCC1020.08	NP_587951.1
	TYW1	XP_001091335.2
Canis lupus familiaris (dog)	TYW1	XP_005620987.1
Saccharomyces cerevisiae (baker's yeast)	TYW1	NP_015117.1
Oryza sativa (rice)	Os03g0775300	NP_001051429.1
Gallus gallus (chicken)	TYW1	XP_003642438.1
Eremothecium gossypii	AGOS_AFL099W	NP_985451.1
Neurospora crassa	NCU06633	XP_961821.1
Arabidopsis thaliana (thale cress)	AT1G75200	NP_177656.2
Xenopus tropicalis (tropical clawed frog)	tyw1	NP_001096267.1
Pan troglodytes (chimpanzee)	TYW1	XP_003318538.1
Danio rerio (zebrafish)	tyw1	NP_001070624.1
Magnaporthe oryzae (rice blast fungus)	MGG_06096	XP_003711962.1
Bos taurus (cattle)	TYW1	NP_001179682.1
Rattus norvegicus (Norway rat)	Tyw1	NP_001100607.1

Gene Ontology

The following LOC479708 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC479708 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe07367	XM_005620930.3 Latest version!	Canis lupus familiaris S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase (LOC479708), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OCe288696	XM_022419526.1 Latest version!	Canis lupus familiaris S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase (LOC479708), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OCe288697	XM_022419527.1 Latest version!	Canis lupus familiaris S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase (LOC479708), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
View Old Accession Versions >>
OCe07367	XM_005620930.2	Canis lupus familiaris S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase (LOC479708), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC479708 ( XM_005620930.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005620987.1 Canis lupus familiaris S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase (LOC479708), mRNA.

Price & Availability↑

CloneID	OCe07367
Clone ID Related Accession (Same CDS sequence)	XM_005620930.2 , XM_005620930.3
Accession Version	XM_005620930.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2145bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726061.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005620930.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATCCTT TTTCGGATAC ATGGGACTTC TCTTCACCTG TGATATCATT ATGGATAAAC 
AGGTTTTACA TATATTTGGG CTTTGCCTTT GGCATTAGCC TTTGGATTTG TTTCCAGATT 
GTCATCAGGA AGCAGTTGTT TATTTTAAAG GGCAAGAAAT CACAGGAGAA ATCTGTTCCA 
AAAGCAACCG GGGATTTGAT GACAAATGGT TGTATCTCTC TTCAAAACAA GGAAGTCTTT 
GTATCTGGAG TGAAGATTTT CTATGGTTCC CAGACTGGAA CAGCAAAGGG ATTTGCAACA 
AGTCTTGCTG AAGCAGTCAC CTCCCTAGAT CTGCCTGTGG CAGTTATTAA TCTGAAAGAA 
TATGATCCAG ATGATCAACT AGTAGAAGAG GTTACTAGTA AAAACGTTTG TGCCTTCGTG 
GTTGCTACAT ATACAGACGG TCAACCCACC GAAAGTGCAG TGTGGTTCTG CAAATGGTTA 
GAGGAAGCAT CCAGTGATTT TCGATTTGGC AAAACTTACC TAAAGGGCAT GAGATACGCT 
GTATTTGGCC TAGGAAATTC TGTCTATGCC AGCCACTTCA ACAAGGTTGG CAAGAATGTT 
GATAAGTGGC TCTGGATGCT TGGTGCTCAT CGTGTGGTGG CTAGAGGAGA GGGGGACTGT 
GACGTGGTTA AAAGCAAACA TGGCAGCATC GAGGCAGACT TCAGAGTTTG GAAGACCAAG 
TTCCTCGCCC GGCTGCAGGC ACTTCAGAAA GGAGAGAAAA AGCCTTGTGA CGGCAACTGC 
AAGAGAGGCC AGTGTGAATC ATCCGTGGAG GAAGAGCCCT CTGAGAGCAC CAGTGAGGAA 
GAATCTGGTA CTGAAGACCA TCAGAATTTA AATTCTGTCG TTGATGTTGA GGACCTTGGC 
AAGATTATGA ATTGTGTGAA GAAAGAAAAG AGAGAAAAGG AGGAACAGGA GGAGAAAAGT 
AGTTTGGTCA GGAACACTGT GAAGAATGAA GACAGTGAAA GAAGAACTAT GATAACCCCT 
GCTCTCCGAG AAGCCCTCAC TAAACAAGGT TATCAGTTGA TTGGGAGCCA CTCTGGGGTG 
AAGCTTTGCA GATGGACAAA GTCAATGCTC CGAGGGAGAG GAGGTTGTTA TAAACATACG 
TTCTATGGCA TCGAAAGCCA TCGTTGCATG GAAACTACCC CAAGCTTGGC TTGTGCAAAT 
AAATGTGTCT TCTGTTGGCG ACACCACACC AATCCGGTGG GCACCGAATG GCGGTGGAAG 
ATGGACCAGC CTGAAATGAT CTTAAAGGAA GCCATTGAAA ACCATCAGAA CATGATTAAG 
CAGTTTAAAG GAGTACCAGG TGTCAAAGAA GAACGTTTTG AAGAAGCAAT GATGGTAAAG 
CACTGTGCAT TGTCCCTTGT GGGAGAACCC ATCATGTACC CAGAAATCAA TAGGTTTTTG 
AAACTACTCC ATGAGTGTAA AATCTCTAGT TTCCTGGTCA CAAATGCACA ATTCCCTCTG 
GAAATCAGGA ACCTCAAACC AGTTACCCAG CTATATGTCA GTGTGGATGC TAGCACCAAA 
GATAGCCTGA AGAAAATCGA CCGCCCACTC TTCAAGGATT TCTGGCAGAG ATTCCTCAAC 
AGCTTAAAAG CCTTGGCAGC AAAGCAACAG CGTACCGTGT ACAGACTGAC TCTAGTGAAA 
GCATGGAATG TGGATGAACT CCAGGCCTAT GCTGAGCTGG TGTCCCTGGG GAATCCTGAC 
TTCATCGAAG TGAAGGGTGT CACCTACTGT GGAGAAAGTT CAGCAAGCAG TCTCACCATG 
GCCAACGTGC CCTGGCATGA GGAGGTGATA CATTTTGTCC GTGAGTTGGT GGATCTGATC 
CCCGATTATG AAATTGCTTG TGAACATGAA CATTCCAACT GTCTCCTGAT AGCTCACAAA 
CGGTTTAAGA TAGGTAGAGA ATGGTGGACA TGGATCGATT ATAACCGCTT CCAAGAGCTC 
GTGCAGGGAT ATGAAGATAG TGGTGGATCA GCAACTTTCA GCGCAGAGGA TTATATGGCG 
AAAACTCCTC ACTGGGCATT ATTTGGTGCC AGTGAAAGAG GTTTTGATCC CAAGGACACA 
CGATATCAGA GAAAGAACAA ATCAAAGGAT ATTTCTGGAT GTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005620987.1
CDS	183..2327
Translation	MDPFSDTWDFSSPVISLWINRFYIYLGFAFGISLWICFQIVIRKQLFILKGKKSQEKSVPKATGDLMTNGCISLQNKEVFVSGVKIFYGSQTGTAKGFATSLAEAVTSLDLPVAVINLKEYDPDDQLVEEVTSKNVCAFVVATYTDGQPTESAVWFCKWLEEASSDFRFGKTYLKGMRYAVFGLGNSVYASHFNKVGKNVDKWLWMLGAHRVVARGEGDCDVVKSKHGSIEADFRVWKTKFLARLQALQKGEKKPCDGNCKRGQCESSVEEEPSESTSEEESGTEDHQNLNSVVDVEDLGKIMNCVKKEKREKEEQEEKSSLVRNTVKNEDSERRTMITPALREALTKQGYQLIGSHSGVKLCRWTKSMLRGRGGCYKHTFYGIESHRCMETTPSLACANKCVFCWRHHTNPVGTEWRWKMDQPEMILKEAIENHQNMIKQFKGVPGVKEERFEEAMMVKHCALSLVGEPIMYPEINRFLKLLHECKISSFLVTNAQFPLEIRNLKPVTQLYVSVDASTKDSLKKIDRPLFKDFWQRFLNSLKALAAKQQRTVYRLTLVKAWNVDELQAYAELVSLGNPDFIEVKGVTYCGESSASSLTMANVPWHEEVIHFVRELVDLIPDYEIACEHEHSNCLLIAHKRFKIGREWWTWIDYNRFQELVQGYEDSGGSATFSAEDYMAKTPHWALFGASERGFDPKDTRYQRKNKSKDISGC

Target ORF information:

RefSeq Version	XM_005620930.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase (LOC479708), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005620930.2

ORF Insert Sequence:

ATGGATCCTT TTTCGGATAC ATGGGACTTC TCTTCACCTG TGATATCATT ATGGATAAAC 
AGGTTTTACA TATATTTGGG CTTTGCCTTT GGCATTAGCC TTTGGATTTG TTTCCAGATT 
GTCATCAGGA AGCAGTTGTT TATTTTAAAG GGCAAGAAAT CACAGGAGAA ATCTGTTCCA 
AAAGCAACCG GGGATTTGAT GACAAATGGT TGTATCTCTC TTCAAAACAA GGAAGTCTTT 
GTATCTGGAG TGAAGATTTT CTATGGTTCC CAGACTGGAA CAGCAAAGGG ATTTGCAACA 
AGTCTTGCTG AAGCAGTCAC CTCCCTAGAT CTGCCTGTGG CAGTTATTAA TCTGAAAGAA 
TATGATCCAG ATGATCAACT AGTAGAAGAG GTTACTAGTA AAAACGTTTG TGCCTTCGTG 
GTTGCTACAT ATACAGACGG TCAACCCACC GAAAGTGCAG TGTGGTTCTG CAAATGGTTA 
GAGGAAGCAT CCAGTGATTT TCGATTTGGC AAAACTTACC TAAAGGGCAT GAGATACGCT 
GTATTTGGCC TAGGAAATTC TGTCTATGCC AGCCACTTCA ACAAGGTTGG CAAGAATGTT 
GATAAGTGGC TCTGGATGCT TGGTGCTCAT CGTGTGGTGG CTAGAGGAGA GGGGGACTGT 
GACGTGGTTA AAAGCAAACA TGGCAGCATC GAGGCAGACT TCAGAGTTTG GAAGACCAAG 
TTCCTCGCCC GGCTGCAGGC ACTTCAGAAA GGAGAGAAAA AGCCTTGTGA CGGCAACTGC 
AAGAGAGGCC AGTGTGAATC ATCCGTGGAG GAAGAGCCCT CTGAGAGCAC CAGTGAGGAA 
GAATCTGGTA CTGAAGACCA TCAGAATTTA AATTCTGTCG TTGATGTTGA GGACCTTGGC 
AAGATTATGA ATTGTGTGAA GAAAGAAAAG AGAGAAAAGG AGGAACAGGA GGAGAAAAGT 
AGTTTGGTCA GGAACACTGT GAAGAATGAA GACAGTGAAA GAAGAACTAT GATAACCCCT 
GCTCTCCGAG AAGCCCTCAC TAAACAAGGT TATCAGTTGA TTGGGAGCCA CTCTGGGGTG 
AAGCTTTGCA GATGGACAAA GTCAATGCTC CGAGGGAGAG GAGGTTGTTA TAAACATACG 
TTCTATGGCA TCGAAAGCCA TCGTTGCATG GAAACTACCC CAAGCTTGGC TTGTGCAAAT 
AAATGTGTCT TCTGTTGGCG ACACCACACC AATCCGGTGG GCACCGAATG GCGGTGGAAG 
ATGGACCAGC CTGAAATGAT CTTAAAGGAA GCCATTGAAA ACCATCAGAA CATGATTAAG 
CAGTTTAAAG GAGTACCAGG TGTCAAAGAA GAACGTTTTG AAGAAGCAAT GATGGTAAAG 
CACTGTGCAT TGTCCCTTGT GGGAGAACCC ATCATGTACC CAGAAATCAA TAGGTTTTTG 
AAACTACTCC ATGAGTGTAA AATCTCTAGT TTCCTGGTCA CAAATGCACA ATTCCCTCTG 
GAAATCAGGA ACCTCAAACC AGTTACCCAG CTATATGTCA GTGTGGATGC TAGCACCAAA 
GATAGCCTGA AGAAAATCGA CCGCCCACTC TTCAAGGATT TCTGGCAGAG ATTCCTCAAC 
AGCTTAAAAG CCTTGGCAGC AAAGCAACAG CGTACCGTGT ACAGACTGAC TCTAGTGAAA 
GCATGGAATG TGGATGAACT CCAGGCCTAT GCTGAGCTGG TGTCCCTGGG GAATCCTGAC 
TTCATCGAAG TGAAGGGTGT CACCTACTGT GGAGAAAGTT CAGCAAGCAG TCTCACCATG 
GCCAACGTGC CCTGGCATGA GGAGGTGATA CATTTTGTCC GTGAGTTGGT GGATCTGATC 
CCCGATTATG AAATTGCTTG TGAACATGAA CATTCCAACT GTCTCCTGAT AGCTCACAAA 
CGGTTTAAGA TAGGTAGAGA ATGGTGGACA TGGATCGATT ATAACCGCTT CCAAGAGCTC 
GTGCAGGGAT ATGAAGATAG TGGTGGATCA GCAACTTTCA GCGCAGAGGA TTATATGGCG 
AAAACTCCTC ACTGGGCATT ATTTGGTGCC AGTGAAAGAG GTTTTGATCC CAAGGACACA 
CGATATCAGA GAAAGAACAA ATCAAAGGAT ATTTCTGGAT GTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC479708 ( XM_005620930.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005620987.1 Canis lupus familiaris S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase (LOC479708), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe07367
Clone ID Related Accession (Same CDS sequence)	XM_005620930.2 , XM_005620930.3
Accession Version	XM_005620930.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2145bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006588.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005620930.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATCCTT TTTCGGATAC ATGGGACTTC TCTTCACCTG TGATATCATT ATGGATAAAC 
AGGTTTTACA TATATTTGGG CTTTGCCTTT GGCATTAGCC TTTGGATTTG TTTCCAGATT 
GTCATCAGGA AGCAGTTGTT TATTTTAAAG GGCAAGAAAT CACAGGAGAA ATCTGTTCCA 
AAAGCAACCG GGGATTTGAT GACAAATGGT TGTATCTCTC TTCAAAACAA GGAAGTCTTT 
GTATCTGGAG TGAAGATTTT CTATGGTTCC CAGACTGGAA CAGCAAAGGG ATTTGCAACA 
AGTCTTGCTG AAGCAGTCAC CTCCCTAGAT CTGCCTGTGG CAGTTATTAA TCTGAAAGAA 
TATGATCCAG ATGATCAACT AGTAGAAGAG GTTACTAGTA AAAACGTTTG TGCCTTCGTG 
GTTGCTACAT ATACAGACGG TCAACCCACC GAAAGTGCAG TGTGGTTCTG CAAATGGTTA 
GAGGAAGCAT CCAGTGATTT TCGATTTGGC AAAACTTACC TAAAGGGCAT GAGATACGCT 
GTATTTGGCC TAGGAAATTC TGTCTATGCC AGCCACTTCA ACAAGGTTGG CAAGAATGTT 
GATAAGTGGC TCTGGATGCT TGGTGCTCAT CGTGTGGTGG CTAGAGGAGA GGGGGACTGT 
GACGTGGTTA AAAGCAAACA TGGCAGCATC GAGGCAGACT TCAGAGTTTG GAAGACCAAG 
TTCCTCGCCC GGCTGCAGGC ACTTCAGAAA GGAGAGAAAA AGCCTTGTGA CGGCAACTGC 
AAGAGAGGCC AGTGTGAATC ATCCGTGGAG GAAGAGCCCT CTGAGAGCAC CAGTGAGGAA 
GAATCTGGTA CTGAAGACCA TCAGAATTTA AATTCTGTCG TTGATGTTGA GGACCTTGGC 
AAGATTATGA ATTGTGTGAA GAAAGAAAAG AGAGAAAAGG AGGAACAGGA GGAGAAAAGT 
AGTTTGGTCA GGAACACTGT GAAGAATGAA GACAGTGAAA GAAGAACTAT GATAACCCCT 
GCTCTCCGAG AAGCCCTCAC TAAACAAGGT TATCAGTTGA TTGGGAGCCA CTCTGGGGTG 
AAGCTTTGCA GATGGACAAA GTCAATGCTC CGAGGGAGAG GAGGTTGTTA TAAACATACG 
TTCTATGGCA TCGAAAGCCA TCGTTGCATG GAAACTACCC CAAGCTTGGC TTGTGCAAAT 
AAATGTGTCT TCTGTTGGCG ACACCACACC AATCCGGTGG GCACCGAATG GCGGTGGAAG 
ATGGACCAGC CTGAAATGAT CTTAAAGGAA GCCATTGAAA ACCATCAGAA CATGATTAAG 
CAGTTTAAAG GAGTACCAGG TGTCAAAGAA GAACGTTTTG AAGAAGCAAT GATGGTAAAG 
CACTGTGCAT TGTCCCTTGT GGGAGAACCC ATCATGTACC CAGAAATCAA TAGGTTTTTG 
AAACTACTCC ATGAGTGTAA AATCTCTAGT TTCCTGGTCA CAAATGCACA ATTCCCTCTG 
GAAATCAGGA ACCTCAAACC AGTTACCCAG CTATATGTCA GTGTGGATGC TAGCACCAAA 
GATAGCCTGA AGAAAATCGA CCGCCCACTC TTCAAGGATT TCTGGCAGAG ATTCCTCAAC 
AGCTTAAAAG CCTTGGCAGC AAAGCAACAG CGTACCGTGT ACAGACTGAC TCTAGTGAAA 
GCATGGAATG TGGATGAACT CCAGGCCTAT GCTGAGCTGG TGTCCCTGGG GAATCCTGAC 
TTCATCGAAG TGAAGGGTGT CACCTACTGT GGAGAAAGTT CAGCAAGCAG TCTCACCATG 
GCCAACGTGC CCTGGCATGA GGAGGTGATA CATTTTGTCC GTGAGTTGGT GGATCTGATC 
CCCGATTATG AAATTGCTTG TGAACATGAA CATTCCAACT GTCTCCTGAT AGCTCACAAA 
CGGTTTAAGA TAGGTAGAGA ATGGTGGACA TGGATCGATT ATAACCGCTT CCAAGAGCTC 
GTGCAGGGAT ATGAAGATAG TGGTGGATCA GCAACTTTCA GCGCAGAGGA TTATATGGCG 
AAAACTCCTC ACTGGGCATT ATTTGGTGCC AGTGAAAGAG GTTTTGATCC CAAGGACACA 
CGATATCAGA GAAAGAACAA ATCAAAGGAT ATTTCTGGAT GTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005620987.1
CDS	189..2333
Translation	MDPFSDTWDFSSPVISLWINRFYIYLGFAFGISLWICFQIVIRKQLFILKGKKSQEKSVPKATGDLMTNGCISLQNKEVFVSGVKIFYGSQTGTAKGFATSLAEAVTSLDLPVAVINLKEYDPDDQLVEEVTSKNVCAFVVATYTDGQPTESAVWFCKWLEEASSDFRFGKTYLKGMRYAVFGLGNSVYASHFNKVGKNVDKWLWMLGAHRVVARGEGDCDVVKSKHGSIEADFRVWKTKFLARLQALQKGEKKPCDGNCKRGQCESSVEEEPSESTSEEESGTEDHQNLNSVVDVEDLGKIMNCVKKEKREKEEQEEKSSLVRNTVKNEDSERRTMITPALREALTKQGYQLIGSHSGVKLCRWTKSMLRGRGGCYKHTFYGIESHRCMETTPSLACANKCVFCWRHHTNPVGTEWRWKMDQPEMILKEAIENHQNMIKQFKGVPGVKEERFEEAMMVKHCALSLVGEPIMYPEINRFLKLLHECKISSFLVTNAQFPLEIRNLKPVTQLYVSVDASTKDSLKKIDRPLFKDFWQRFLNSLKALAAKQQRTVYRLTLVKAWNVDELQAYAELVSLGNPDFIEVKGVTYCGESSASSLTMANVPWHEEVIHFVRELVDLIPDYEIACEHEHSNCLLIAHKRFKIGREWWTWIDYNRFQELVQGYEDSGGSATFSAEDYMAKTPHWALFGASERGFDPKDTRYQRKNKSKDISGC

Target ORF information:

RefSeq Version	XM_005620930.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase (LOC479708), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005620930.3

ORF Insert Sequence:

ATGGATCCTT TTTCGGATAC ATGGGACTTC TCTTCACCTG TGATATCATT ATGGATAAAC 
AGGTTTTACA TATATTTGGG CTTTGCCTTT GGCATTAGCC TTTGGATTTG TTTCCAGATT 
GTCATCAGGA AGCAGTTGTT TATTTTAAAG GGCAAGAAAT CACAGGAGAA ATCTGTTCCA 
AAAGCAACCG GGGATTTGAT GACAAATGGT TGTATCTCTC TTCAAAACAA GGAAGTCTTT 
GTATCTGGAG TGAAGATTTT CTATGGTTCC CAGACTGGAA CAGCAAAGGG ATTTGCAACA 
AGTCTTGCTG AAGCAGTCAC CTCCCTAGAT CTGCCTGTGG CAGTTATTAA TCTGAAAGAA 
TATGATCCAG ATGATCAACT AGTAGAAGAG GTTACTAGTA AAAACGTTTG TGCCTTCGTG 
GTTGCTACAT ATACAGACGG TCAACCCACC GAAAGTGCAG TGTGGTTCTG CAAATGGTTA 
GAGGAAGCAT CCAGTGATTT TCGATTTGGC AAAACTTACC TAAAGGGCAT GAGATACGCT 
GTATTTGGCC TAGGAAATTC TGTCTATGCC AGCCACTTCA ACAAGGTTGG CAAGAATGTT 
GATAAGTGGC TCTGGATGCT TGGTGCTCAT CGTGTGGTGG CTAGAGGAGA GGGGGACTGT 
GACGTGGTTA AAAGCAAACA TGGCAGCATC GAGGCAGACT TCAGAGTTTG GAAGACCAAG 
TTCCTCGCCC GGCTGCAGGC ACTTCAGAAA GGAGAGAAAA AGCCTTGTGA CGGCAACTGC 
AAGAGAGGCC AGTGTGAATC ATCCGTGGAG GAAGAGCCCT CTGAGAGCAC CAGTGAGGAA 
GAATCTGGTA CTGAAGACCA TCAGAATTTA AATTCTGTCG TTGATGTTGA GGACCTTGGC 
AAGATTATGA ATTGTGTGAA GAAAGAAAAG AGAGAAAAGG AGGAACAGGA GGAGAAAAGT 
AGTTTGGTCA GGAACACTGT GAAGAATGAA GACAGTGAAA GAAGAACTAT GATAACCCCT 
GCTCTCCGAG AAGCCCTCAC TAAACAAGGT TATCAGTTGA TTGGGAGCCA CTCTGGGGTG 
AAGCTTTGCA GATGGACAAA GTCAATGCTC CGAGGGAGAG GAGGTTGTTA TAAACATACG 
TTCTATGGCA TCGAAAGCCA TCGTTGCATG GAAACTACCC CAAGCTTGGC TTGTGCAAAT 
AAATGTGTCT TCTGTTGGCG ACACCACACC AATCCGGTGG GCACCGAATG GCGGTGGAAG 
ATGGACCAGC CTGAAATGAT CTTAAAGGAA GCCATTGAAA ACCATCAGAA CATGATTAAG 
CAGTTTAAAG GAGTACCAGG TGTCAAAGAA GAACGTTTTG AAGAAGCAAT GATGGTAAAG 
CACTGTGCAT TGTCCCTTGT GGGAGAACCC ATCATGTACC CAGAAATCAA TAGGTTTTTG 
AAACTACTCC ATGAGTGTAA AATCTCTAGT TTCCTGGTCA CAAATGCACA ATTCCCTCTG 
GAAATCAGGA ACCTCAAACC AGTTACCCAG CTATATGTCA GTGTGGATGC TAGCACCAAA 
GATAGCCTGA AGAAAATCGA CCGCCCACTC TTCAAGGATT TCTGGCAGAG ATTCCTCAAC 
AGCTTAAAAG CCTTGGCAGC AAAGCAACAG CGTACCGTGT ACAGACTGAC TCTAGTGAAA 
GCATGGAATG TGGATGAACT CCAGGCCTAT GCTGAGCTGG TGTCCCTGGG GAATCCTGAC 
TTCATCGAAG TGAAGGGTGT CACCTACTGT GGAGAAAGTT CAGCAAGCAG TCTCACCATG 
GCCAACGTGC CCTGGCATGA GGAGGTGATA CATTTTGTCC GTGAGTTGGT GGATCTGATC 
CCCGATTATG AAATTGCTTG TGAACATGAA CATTCCAACT GTCTCCTGAT AGCTCACAAA 
CGGTTTAAGA TAGGTAGAGA ATGGTGGACA TGGATCGATT ATAACCGCTT CCAAGAGCTC 
GTGCAGGGAT ATGAAGATAG TGGTGGATCA GCAACTTTCA GCGCAGAGGA TTATATGGCG 
AAAACTCCTC ACTGGGCATT ATTTGGTGCC AGTGAAAGAG GTTTTGATCC CAAGGACACA 
CGATATCAGA GAAAGAACAA ATCAAAGGAT ATTTCTGGAT GTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC479708 ( XM_022419526.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022275234.1 Canis lupus familiaris S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase (LOC479708), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCe288696
Clone ID Related Accession (Same CDS sequence)	XM_022419526.1
Accession Version	XM_022419526.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2013bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006588.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATCCTT TTTCGGATAC ATGGGACTTC TCTTCACCTG TGATATCATT ATGGATAAAC 
AGGTTTTACA TATATTTGGG CTTTGCCTTT GGCATTAGCC TTTGGATTTG TTTCCAGATT 
GTCATCAGGA AGCAGTTGTT TATTTTAAAG GGCAAGAAAT CACAGGAGAA ATCTGTTCCA 
AAAGCAACCG GGGATTTGAT GACAAATGGT TGTATCTCTC TTCAAAACAA GGAAGTCTTT 
GTATCTGGAG TGAAGATTTT CTATGGTTCC CAGACTGGAA CAGCAAAGGG ATTTGCAACA 
AGTCTTGCTG AAGCAGTCAC CTCCCTAGAT CTGCCTGTGG CAGTTATTAA TCTGAAAGAA 
TATGATCCAG ATGATCAACT AGTAGAAGAG GTTACTAGTA AAAACGTTTG TGCCTTCGTG 
GTTGCTACAT ATACAGACGG TCAACCCACC GAAAGTGCAG TGTGGTTCTG CAAATGGTTA 
GAGGAAGCAT CCAGTGATTT TCGATTTGGC AAAACTTACC TAAAGGGCAT GAGATACGCT 
GTATTTGGCC TAGGAAATTC TGTCTATGCC AGCCACTTCA ACAAGGTTGG CAAGAATGTT 
GATAAGTGGC TCTGGATGCT TGGTGCTCAT CGTGTGGTGG CTAGAGGAGA GGGGGACTGT 
GACGTGGTTA AAAGCAAACA TGGCAGCATC GAGGCAGACT TCAGAGTTTG GAAGACCAAG 
TTCCTCGCCC GGCTGCAGGC ACTTCAGAAA GGAGAGAAAA AGCCTTGTGA CGGCAACTGC 
AAGAGAGGCC AGTGTGAATC ATCCGTGGAG GAAGAGCCCT CTGAGAGCAC CAGTGAGGAA 
GAATCTGGTA CTGAAGACCA TCAGAATTTA AATTCTGTCG TTGATGTTGA GGACCTTGGC 
AAGATTATGA ATTGTGTGAA GAAAGAAAAG AGAGAAAAGG AGGAACAGGA GGAGAAAAGT 
AGTTTGGTCA GGAACACTGT GAAGAATGAA GACAGTGAAA GAAGAACTAT GATAACCCCT 
GCTCTCCGAG AAGCCCTCAC TAAACAAGGT TATCAGTTGA TTGGGAGCCA CTCTGGGGTG 
AAGCTTTGCA GATGGACAAA GTCAATGCTC CGAGGGAGAG GAGGTTGTTA TAAACATACG 
TTCTATGGCA TCGAAAGCCA TCGTTGCATG GAAACTACCC CAAGCTTGGC TTGTGCAAAT 
AAATGTGTCT TCTGTTGGCG ACACCACACC AATCCGGTGG GCACCGAATG GCGGTGGAAG 
ATGGACCAGC CTGAAATGAT CTTAAAGGAA GCCATTGAAA ACCATCAGAA CATGATTAAG 
CAGTTTAAAG GAGTACCAGG TGTCAAAGAA GAACGTTTTG AAGAAGCAAT GATGGTAAAG 
CACTGTGCAT TGTCCCTTGT GGGAGAACCC ATCATGTACC CAGAAATCAA TAGGTTTTTG 
AAACTACTCC ATGAGTGTAA AATCTCTAGT TTCCTGGTCA CAAATGCACA ATTCCCTCTG 
GAAATCAGGA ACCTCAAACC AGTTACCCAG CTATATGTCA GTGTGGATGC TAGCACCAAA 
GATAGCCTGA AGAAAATCGA CCGCCCACTC TTCAAGGATT TCTGGCAGAG ATTCCTCAAC 
AGCTTAAAAG CCTTGGCAGC AAAGCAACAG CGTACCGTGT ACAGACTGAC TCTAGTGAAA 
GCATGGAATG TGGATGAACT CCAGGCCTAT GCTGAGCTGG TGTCCCTGGG GAATCCTGAC 
TTCATCGAAG TGAAGGGTGT CACCTACTGT GGAGAAAGTT CAGCAAGCAG TCTCACCATG 
GCCAACGTGC CCTGGCATGA GGAGGTGATA CATTTTGTCC GTGAGTTGGT GGATCTGATC 
CCCGATTATG AAATTGCTTG TGAACATGAA CATTCCAACT GTCTCCTGAT AGCTCACAAA 
CGGACTTCAC CTAGCTTCTT GAAGTGGTAT CTGGTGTATA ACTGGTGCAT GCTCAAAAAC 
AAAAGCAAAA AACACCTTTG TCATAACCTC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022275234.1
CDS	190..2202
Translation	MDPFSDTWDFSSPVISLWINRFYIYLGFAFGISLWICFQIVIRKQLFILKGKKSQEKSVPKATGDLMTNGCISLQNKEVFVSGVKIFYGSQTGTAKGFATSLAEAVTSLDLPVAVINLKEYDPDDQLVEEVTSKNVCAFVVATYTDGQPTESAVWFCKWLEEASSDFRFGKTYLKGMRYAVFGLGNSVYASHFNKVGKNVDKWLWMLGAHRVVARGEGDCDVVKSKHGSIEADFRVWKTKFLARLQALQKGEKKPCDGNCKRGQCESSVEEEPSESTSEEESGTEDHQNLNSVVDVEDLGKIMNCVKKEKREKEEQEEKSSLVRNTVKNEDSERRTMITPALREALTKQGYQLIGSHSGVKLCRWTKSMLRGRGGCYKHTFYGIESHRCMETTPSLACANKCVFCWRHHTNPVGTEWRWKMDQPEMILKEAIENHQNMIKQFKGVPGVKEERFEEAMMVKHCALSLVGEPIMYPEINRFLKLLHECKISSFLVTNAQFPLEIRNLKPVTQLYVSVDASTKDSLKKIDRPLFKDFWQRFLNSLKALAAKQQRTVYRLTLVKAWNVDELQAYAELVSLGNPDFIEVKGVTYCGESSASSLTMANVPWHEEVIHFVRELVDLIPDYEIACEHEHSNCLLIAHKRTSPSFLKWYLVYNWCMLKNKSKKHLCHNL

Target ORF information:

RefSeq Version	XM_022419526.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase (LOC479708), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022419526.1

ORF Insert Sequence:

ATGGATCCTT TTTCGGATAC ATGGGACTTC TCTTCACCTG TGATATCATT ATGGATAAAC 
AGGTTTTACA TATATTTGGG CTTTGCCTTT GGCATTAGCC TTTGGATTTG TTTCCAGATT 
GTCATCAGGA AGCAGTTGTT TATTTTAAAG GGCAAGAAAT CACAGGAGAA ATCTGTTCCA 
AAAGCAACCG GGGATTTGAT GACAAATGGT TGTATCTCTC TTCAAAACAA GGAAGTCTTT 
GTATCTGGAG TGAAGATTTT CTATGGTTCC CAGACTGGAA CAGCAAAGGG ATTTGCAACA 
AGTCTTGCTG AAGCAGTCAC CTCCCTAGAT CTGCCTGTGG CAGTTATTAA TCTGAAAGAA 
TATGATCCAG ATGATCAACT AGTAGAAGAG GTTACTAGTA AAAACGTTTG TGCCTTCGTG 
GTTGCTACAT ATACAGACGG TCAACCCACC GAAAGTGCAG TGTGGTTCTG CAAATGGTTA 
GAGGAAGCAT CCAGTGATTT TCGATTTGGC AAAACTTACC TAAAGGGCAT GAGATACGCT 
GTATTTGGCC TAGGAAATTC TGTCTATGCC AGCCACTTCA ACAAGGTTGG CAAGAATGTT 
GATAAGTGGC TCTGGATGCT TGGTGCTCAT CGTGTGGTGG CTAGAGGAGA GGGGGACTGT 
GACGTGGTTA AAAGCAAACA TGGCAGCATC GAGGCAGACT TCAGAGTTTG GAAGACCAAG 
TTCCTCGCCC GGCTGCAGGC ACTTCAGAAA GGAGAGAAAA AGCCTTGTGA CGGCAACTGC 
AAGAGAGGCC AGTGTGAATC ATCCGTGGAG GAAGAGCCCT CTGAGAGCAC CAGTGAGGAA 
GAATCTGGTA CTGAAGACCA TCAGAATTTA AATTCTGTCG TTGATGTTGA GGACCTTGGC 
AAGATTATGA ATTGTGTGAA GAAAGAAAAG AGAGAAAAGG AGGAACAGGA GGAGAAAAGT 
AGTTTGGTCA GGAACACTGT GAAGAATGAA GACAGTGAAA GAAGAACTAT GATAACCCCT 
GCTCTCCGAG AAGCCCTCAC TAAACAAGGT TATCAGTTGA TTGGGAGCCA CTCTGGGGTG 
AAGCTTTGCA GATGGACAAA GTCAATGCTC CGAGGGAGAG GAGGTTGTTA TAAACATACG 
TTCTATGGCA TCGAAAGCCA TCGTTGCATG GAAACTACCC CAAGCTTGGC TTGTGCAAAT 
AAATGTGTCT TCTGTTGGCG ACACCACACC AATCCGGTGG GCACCGAATG GCGGTGGAAG 
ATGGACCAGC CTGAAATGAT CTTAAAGGAA GCCATTGAAA ACCATCAGAA CATGATTAAG 
CAGTTTAAAG GAGTACCAGG TGTCAAAGAA GAACGTTTTG AAGAAGCAAT GATGGTAAAG 
CACTGTGCAT TGTCCCTTGT GGGAGAACCC ATCATGTACC CAGAAATCAA TAGGTTTTTG 
AAACTACTCC ATGAGTGTAA AATCTCTAGT TTCCTGGTCA CAAATGCACA ATTCCCTCTG 
GAAATCAGGA ACCTCAAACC AGTTACCCAG CTATATGTCA GTGTGGATGC TAGCACCAAA 
GATAGCCTGA AGAAAATCGA CCGCCCACTC TTCAAGGATT TCTGGCAGAG ATTCCTCAAC 
AGCTTAAAAG CCTTGGCAGC AAAGCAACAG CGTACCGTGT ACAGACTGAC TCTAGTGAAA 
GCATGGAATG TGGATGAACT CCAGGCCTAT GCTGAGCTGG TGTCCCTGGG GAATCCTGAC 
TTCATCGAAG TGAAGGGTGT CACCTACTGT GGAGAAAGTT CAGCAAGCAG TCTCACCATG 
GCCAACGTGC CCTGGCATGA GGAGGTGATA CATTTTGTCC GTGAGTTGGT GGATCTGATC 
CCCGATTATG AAATTGCTTG TGAACATGAA CATTCCAACT GTCTCCTGAT AGCTCACAAA 
CGGACTTCAC CTAGCTTCTT GAAGTGGTAT CTGGTGTATA ACTGGTGCAT GCTCAAAAAC 
AAAAGCAAAA AACACCTTTG TCATAACCTC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC479708 ( XM_022419527.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022275235.1 Canis lupus familiaris S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase (LOC479708), transcript variant X3, mRNA.

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CloneID	OCe288697
Clone ID Related Accession (Same CDS sequence)	XM_022419527.1
Accession Version	XM_022419527.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1926bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006588.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATCCTT TTTCGGATAC ATGGGACTTC TCTTCACCTG TGATATCATT ATGGATAAAC 
AGGTTTTACA TATATTTGGG CTTTGCCTTT GGCATTAGCC TTTGGATTTG TTTCCAGATT 
GTCATCAGGA AGCAGTTGTT TATTTTAAAG GGCAAGAAAT CACAGGAGAA ATCTGTTCCA 
AAAGCAACCG GGGATTTGAT GACAAATGGT TGTATCTCTC TTCAAAACAA GGAAGTCTTT 
GTATCTGGAG TGAAGATTTT CTATGGTTCC CAGACTGGAA CAGCAAAGGG ATTTGCAACA 
AGTCTTGCTG AAGCAGTCAC CTCCCTAGAT CTGCCTGTGG CAGTTATTAA TCTGAAAGAA 
TATGATCCAG ATGATCAACT AGTAGAAGAG GTTACTAGTA AAAACGTTTG TGCCTTCGTG 
GTTGCTACAT ATACAGACGG TCAACCCACC GAAAGTGCAG TGTGGTTCTG CAAATGGTTA 
GAGGAAGCAT CCAGTGATTT TCGATTTGGC AAAACTTACC TAAAGGGCAT GAGATACGCT 
GTATTTGGCC TAGGAAATTC TGTCTATGCC AGCCACTTCA ACAAGGTTGG CAAGAATGTT 
GATAAGTGGC TCTGGATGCT TGGTGCTCAT CGTGTGGTGG CTAGAGGAGA GGGGGACTGT 
GACGTGGTTA AAAGCAAACA TGGCAGCATC GAGGCAGACT TCAGAGTTTG GAAGACCAAG 
TTCCTCGCCC GGCTGCAGGC ACTTCAGAAA GGAGAGAAAA AGCCTTGTGA CGGCAACTGC 
AAGAGAGGCC AGTGTGAATC ATCCGTGGAG GAAGAGCCCT CTGAGAGCAC CAGTGAGGAA 
GAATCTGGTA CTGAAGACCA TCAGAATTTA AATTCTGTCG TTGATGTTGA GGACCTTGGC 
AAGATTATGA ATTGTGTGAA GAAAGAAAAG AGAGAAAAGG AGGAACAGGA GGAGAAAAGT 
AGTTTGGTCA GGAACACTGT GAAGAATGAA GACAGTGAAA GAAGAACTAT GATAACCCCT 
GCTCTCCGAG AAGCCCTCAC TAAACAAGGT TATCAGTTGA TTGGGAGCCA CTCTGGGGTG 
AAGCTTTGCA GATGGACAAA GTCAATGCTC CGAGGGAGAG GAGGTTGTTA TAAACATACG 
TTCTATGGCA TCGAAAGCCA TCGTTGCATG GAAACTACCC CAAGCTTGGC TTGTGCAAAT 
AAATGTGTCT TCTGTTGGCG ACACCACACC AATCCGGTGG GCACCGAATG GCGGTGGAAG 
ATGGACCAGC CTGAAATGAT CTTAAAGGAA GCCATTGAAA ACCATCAGAA CATGATTAAG 
CAGTTTAAAG GAGTACCAGG TGTCAAAGAA GAACGTTTTG AAGAAGCAAT GATGGTAAAG 
CACTGTGCAT TGTCCCTTGT GGGAGAACCC ATCATGTACC CAGAAATCAA TAGGTTTTTG 
AAACTACTCC ATGAGTGTAA AATCTCTAGT TTCCTGGTCA CAAATGCACA ATTCCCTCTG 
GAAATCAGGA ACCTCAAACC AGTTACCCAG CTATATGTCA GTGTGGATGC TAGCACCAAA 
GATAGCCTGA AGAAAATCGA CCGCCCACTC TTCAAGGATT TCTGGCAGAG ATTCCTCAAC 
AGCTTAAAAG CCTTGGCAGC AAAGCAACAG CGTACCGTGT ACAGACTGAC TCTAGTGAAA 
GCATGGAATG TGGATGAACT CCAGGCCTAT GCTGAGCTGG TGTCCCTGGG GAATCCTGAC 
TTCATCGAAG TGAAGGGTGT CACCTACTGT GGAGAAAGTT CAGCAAGCAG TCTCACCATG 
GCCAACGTGC CCTGGCATGA GGAGGTGATA CATTTTGTCC GTGAGTTGGT GGATCTGATC 
CCCGATTATG AAATTGCTTG TGAACATGAA CATTCCAACT GTCTCCTGAT AGCTCACAAA 
CGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022275235.1
CDS	190..2115
Translation	MDPFSDTWDFSSPVISLWINRFYIYLGFAFGISLWICFQIVIRKQLFILKGKKSQEKSVPKATGDLMTNGCISLQNKEVFVSGVKIFYGSQTGTAKGFATSLAEAVTSLDLPVAVINLKEYDPDDQLVEEVTSKNVCAFVVATYTDGQPTESAVWFCKWLEEASSDFRFGKTYLKGMRYAVFGLGNSVYASHFNKVGKNVDKWLWMLGAHRVVARGEGDCDVVKSKHGSIEADFRVWKTKFLARLQALQKGEKKPCDGNCKRGQCESSVEEEPSESTSEEESGTEDHQNLNSVVDVEDLGKIMNCVKKEKREKEEQEEKSSLVRNTVKNEDSERRTMITPALREALTKQGYQLIGSHSGVKLCRWTKSMLRGRGGCYKHTFYGIESHRCMETTPSLACANKCVFCWRHHTNPVGTEWRWKMDQPEMILKEAIENHQNMIKQFKGVPGVKEERFEEAMMVKHCALSLVGEPIMYPEINRFLKLLHECKISSFLVTNAQFPLEIRNLKPVTQLYVSVDASTKDSLKKIDRPLFKDFWQRFLNSLKALAAKQQRTVYRLTLVKAWNVDELQAYAELVSLGNPDFIEVKGVTYCGESSASSLTMANVPWHEEVIHFVRELVDLIPDYEIACEHEHSNCLLIAHKR

Target ORF information:

RefSeq Version	XM_022419527.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase (LOC479708), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022419527.1

ORF Insert Sequence:

ATGGATCCTT TTTCGGATAC ATGGGACTTC TCTTCACCTG TGATATCATT ATGGATAAAC 
AGGTTTTACA TATATTTGGG CTTTGCCTTT GGCATTAGCC TTTGGATTTG TTTCCAGATT 
GTCATCAGGA AGCAGTTGTT TATTTTAAAG GGCAAGAAAT CACAGGAGAA ATCTGTTCCA 
AAAGCAACCG GGGATTTGAT GACAAATGGT TGTATCTCTC TTCAAAACAA GGAAGTCTTT 
GTATCTGGAG TGAAGATTTT CTATGGTTCC CAGACTGGAA CAGCAAAGGG ATTTGCAACA 
AGTCTTGCTG AAGCAGTCAC CTCCCTAGAT CTGCCTGTGG CAGTTATTAA TCTGAAAGAA 
TATGATCCAG ATGATCAACT AGTAGAAGAG GTTACTAGTA AAAACGTTTG TGCCTTCGTG 
GTTGCTACAT ATACAGACGG TCAACCCACC GAAAGTGCAG TGTGGTTCTG CAAATGGTTA 
GAGGAAGCAT CCAGTGATTT TCGATTTGGC AAAACTTACC TAAAGGGCAT GAGATACGCT 
GTATTTGGCC TAGGAAATTC TGTCTATGCC AGCCACTTCA ACAAGGTTGG CAAGAATGTT 
GATAAGTGGC TCTGGATGCT TGGTGCTCAT CGTGTGGTGG CTAGAGGAGA GGGGGACTGT 
GACGTGGTTA AAAGCAAACA TGGCAGCATC GAGGCAGACT TCAGAGTTTG GAAGACCAAG 
TTCCTCGCCC GGCTGCAGGC ACTTCAGAAA GGAGAGAAAA AGCCTTGTGA CGGCAACTGC 
AAGAGAGGCC AGTGTGAATC ATCCGTGGAG GAAGAGCCCT CTGAGAGCAC CAGTGAGGAA 
GAATCTGGTA CTGAAGACCA TCAGAATTTA AATTCTGTCG TTGATGTTGA GGACCTTGGC 
AAGATTATGA ATTGTGTGAA GAAAGAAAAG AGAGAAAAGG AGGAACAGGA GGAGAAAAGT 
AGTTTGGTCA GGAACACTGT GAAGAATGAA GACAGTGAAA GAAGAACTAT GATAACCCCT 
GCTCTCCGAG AAGCCCTCAC TAAACAAGGT TATCAGTTGA TTGGGAGCCA CTCTGGGGTG 
AAGCTTTGCA GATGGACAAA GTCAATGCTC CGAGGGAGAG GAGGTTGTTA TAAACATACG 
TTCTATGGCA TCGAAAGCCA TCGTTGCATG GAAACTACCC CAAGCTTGGC TTGTGCAAAT 
AAATGTGTCT TCTGTTGGCG ACACCACACC AATCCGGTGG GCACCGAATG GCGGTGGAAG 
ATGGACCAGC CTGAAATGAT CTTAAAGGAA GCCATTGAAA ACCATCAGAA CATGATTAAG 
CAGTTTAAAG GAGTACCAGG TGTCAAAGAA GAACGTTTTG AAGAAGCAAT GATGGTAAAG 
CACTGTGCAT TGTCCCTTGT GGGAGAACCC ATCATGTACC CAGAAATCAA TAGGTTTTTG 
AAACTACTCC ATGAGTGTAA AATCTCTAGT TTCCTGGTCA CAAATGCACA ATTCCCTCTG 
GAAATCAGGA ACCTCAAACC AGTTACCCAG CTATATGTCA GTGTGGATGC TAGCACCAAA 
GATAGCCTGA AGAAAATCGA CCGCCCACTC TTCAAGGATT TCTGGCAGAG ATTCCTCAAC 
AGCTTAAAAG CCTTGGCAGC AAAGCAACAG CGTACCGTGT ACAGACTGAC TCTAGTGAAA 
GCATGGAATG TGGATGAACT CCAGGCCTAT GCTGAGCTGG TGTCCCTGGG GAATCCTGAC 
TTCATCGAAG TGAAGGGTGT CACCTACTGT GGAGAAAGTT CAGCAAGCAG TCTCACCATG 
GCCAACGTGC CCTGGCATGA GGAGGTGATA CATTTTGTCC GTGAGTTGGT GGATCTGATC 
CCCGATTATG AAATTGCTTG TGAACATGAA CATTCCAACT GTCTCCTGAT AGCTCACAAA 
CGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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LOC479708 cDNA ORF clone, Canis lupus familiaris(dog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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FAX

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