Tyw1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Tyw1
Entrez Gene ID	100929
Full Name	tRNA-yW synthesizing protein 1 homolog (S. cerevisiae)
Synonyms	AW125391,Rsafd1
General protein information	Preferred Names tRNA-yW synthesizing protein 1 homolog (S. cerevisiae) Names S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase radical S-adenosyl methionine and flavodoxin domain-containing protein 1 radical S-adenosyl methionine and flavodoxin domains 1 tRNA wybutosine-synthesizing protein 1 homolog tRNA-yW-synthesizing protein
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 5 Map Location: 5\|5 G1.3

mRNA and Protein(s)

mRNA	Protein	Name
XM_017320584.1	XP_017176073.1	S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase isoform X2
XM_006504331.1	XP_006504394.1	S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase isoform X1
NM_001015876.2	NP_001015876.1	S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase TYW1 isoform 1
XM_006504331.2	XP_006504394.1	S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase isoform X1
NM_178897.4	NP_849228.1	S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase TYW1 isoform 2
XM_006504331.3	XP_006504394.1	S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase isoform X1
XM_017320584.2	XP_017176073.1	S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase isoform X2

Pathways from BioSystems

Homologies

Homo sapiens (human)	TYW1	NP_060734.2
Mus musculus (house mouse)	Tyw1	NP_001015876.1
Schizosaccharomyces pombe (fission yeast)	SPCC1020.08	NP_587951.1
	TYW1	XP_001091335.2
Canis lupus familiaris (dog)	TYW1	XP_005620987.1
Saccharomyces cerevisiae (baker's yeast)	TYW1	NP_015117.1
Oryza sativa (rice)	Os03g0775300	NP_001051429.1
Gallus gallus (chicken)	TYW1	XP_003642438.1
Eremothecium gossypii	AGOS_AFL099W	NP_985451.1
Neurospora crassa	NCU06633	XP_961821.1
Arabidopsis thaliana (thale cress)	AT1G75200	NP_177656.2
Xenopus tropicalis (tropical clawed frog)	tyw1	NP_001096267.1
Pan troglodytes (chimpanzee)	TYW1	XP_003318538.1
Danio rerio (zebrafish)	tyw1	NP_001070624.1
Magnaporthe oryzae (rice blast fungus)	MGG_06096	XP_003711962.1
Bos taurus (cattle)	TYW1	NP_001179682.1
Rattus norvegicus (Norway rat)	Tyw1	NP_001100607.1

Gene Ontology
Bibliography

Related articles in PubMed

BayGenomics: a resource of insertional mutations in mouse embryonic stem cells.
Stryke D, Kawamoto M, Huang CC, Johns SJ, King LA, Harper CA, Meng EC, Lee RE, Yee A, L'Italien L, Chuang PT, Young SG, Skarnes WC, Babbitt PC, Ferrin TE
Nucleic acids research31(1)278-81(2003 Jan)

Construction of long-transcript enriched cDNA libraries from submicrogram amounts of total RNAs by a universal PCR amplification method.
Piao Y, Ko NT, Lim MK, Ko MS
Genome research11(9)1553-8(2001 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Tyw1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Tyw1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu12990	NM_178897.4 Latest version!	Mus musculus tRNA-yW synthesizing protein 1 homolog (S. cerevisiae) (Tyw1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$272.30 $389.00
OMu12980	NM_001015876.2 Latest version!	Mus musculus tRNA-yW synthesizing protein 1 homolog (S. cerevisiae) (Tyw1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$391.30 $559.00
OMu12980	XM_006504331.3 Latest version!	Mus musculus tRNA-yW synthesizing protein 1 homolog (S. cerevisiae) (Tyw1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OMu81205	XM_017320584.2 Latest version!	Mus musculus tRNA-yW synthesizing protein 1 homolog (S. cerevisiae) (Tyw1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
OMu12980	XM_006504331.1	Mus musculus tRNA-yW synthesizing protein 1 homolog (S. cerevisiae) (Tyw1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$391.30 $559.00
OMu12980	XM_006504331.2	Mus musculus tRNA-yW synthesizing protein 1 homolog (S. cerevisiae) (Tyw1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$391.30 $559.00
OMu81205	XM_017320584.1	Mus musculus tRNA-yW synthesizing protein 1 homolog (S. cerevisiae) (Tyw1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	10-12	$307.30 $439.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Tyw1 ( NM_178897.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_849228.1 Mus musculus tRNA-yW synthesizing protein 1 homolog (S. cerevisiae) (Tyw1), transcript variant 2, mRNA.

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CloneID	OMu12990
Clone ID Related Accession (Same CDS sequence)	NM_178897.4
Accession Version	NM_178897.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1365bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-10
Organism	Mus musculus(house mouse)
Product	S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase TYW1 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK045279.1 and AC113029.23. On Aug 1, 2009 this sequence version replaced NM_178897.3. Transcript Variant: This variant (2) has multiple differences in its 3' end, compared to variant 1, which results in a protein (isoform 2) with a shorter and distinct C-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AK045279.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGGGTCCTC TGGATGTGTG GGACTTGTCG CCTTTGCTGT CACTGTGGAT GAACAGGTTC 
TACATATATA TGGGCTGTGC CCTGGGCCTT ACCCTTTGCA TCTGTGTCCA GATCATCAAG 
AAGCAGGTCA CTCGTTCCCA GGAGAAACGT GTCCCAGGAG CTCCTGATAG CTCCCTCTCC 
CCTCAGAAGA AGCAGACACA CGTTTCTGGA GTGAAGATTT TTTACGGATC TCAGACGGGC 
ACTGCCAAGG GATTTGCAGT GGTTCTTGCC AAAGCTGTCA CCTCTCTGGA CCTGCCTGTG 
GCCATTATTA ATCTGAAGGA ATATGATCCA GATGACAGTC TGATAGGAGA GATCACTAGT 
AAAACCGTCT GTGCCTTCCT GGTCGCTACG TACACGGACG GCTGCCCTAC AGAGAGTGCA 
GAGTGGTTCT GCAAGTGGTT AGAGGAATCA GCTAATGATT TCCGCTTTGG CAAAACTTAC 
CTGAAGGGAC TTCGCTATGC AGTGTTTGGC CTGGGAGACT CTGCCTACCG GAGCCACTTC 
AACAAGGTCA GCACGAACGT TGACAAATGG CTCTGGATGC TCGGTGCCCA GCGGGTGCTG 
ACCCGAGGGG AAGGCGACTG CAATGCAGTT CAGAGCAAAC ATGGCAGCAT CGAGGCCGAC 
TTCACAGCCT GGAAGACCAA GTTCATCTCC CGGCTGCAGG CCCTGCAGAG AGGGGAGAAA 
AAGGCTTGTG GCGGGAACTG CAAGAGAGGG AAGTGTGAGT CGGCCCAGCA TGGCCCAGGA 
GAGGCGCGGC CTCACCCGCA AGGCGAATTG CATCCTGGGG ATGCTGAGGA GGAAGAGCCC 
TGTGAGAGCA GCAGCGAGGA CGAGCTCGGG ACCCAGGATT ATCAGAGCCT GACTTCTGTG 
GTGGATGTTG AGGACCTGGG CAACATCATG AACCCCGTGA AGAGGGAAAA GAGAGAAAAG 
TCTCATCAGG ACGGGAAAGC TGCCATGCAG AGGAACCCAG AGAAGACTGA GGACGGCGAA 
GGAAGAGCTA TGATAACACC TGCTCTGCGG GAAGCCCTGA CTAAACAAGG GTACCAGCTG 
ATTGGCAGCC ACTCTGGTGT GAAGCTTTGC AGGTGGACAA AGGTATTTAT TGGTTTTATT 
CAAATATTAT TTCTATTTTC TGCAACTTAT TTATTTTTGT CCATTTATTT TGTGTGTGCG 
CACATGTGTG GCGGTCAGAG CACAGCCTAC AGGATTTGGT TCTCTTTGTG GGCTCTGGGG 
ACCAAACTGT CTTTTAGTGT GACAGCAGCC ACTTGGATGG CTCCGGTATG GTTCTCAGCA 
GTGCTGTTGT GGTATACTTC ACATGCAGTA CGTGGCTCAC GTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_849228.1
CDS	24..1388
Translation	MGPLDVWDLSPLLSLWMNRFYIYMGCALGLTLCICVQIIKKQVTRSQEKRVPGAPDSSLSPQKKQTHVSGVKIFYGSQTGTAKGFAVVLAKAVTSLDLPVAIINLKEYDPDDSLIGEITSKTVCAFLVATYTDGCPTESAEWFCKWLEESANDFRFGKTYLKGLRYAVFGLGDSAYRSHFNKVSTNVDKWLWMLGAQRVLTRGEGDCNAVQSKHGSIEADFTAWKTKFISRLQALQRGEKKACGGNCKRGKCESAQHGPGEARPHPQGELHPGDAEEEEPCESSSEDELGTQDYQSLTSVVDVEDLGNIMNPVKREKREKSHQDGKAAMQRNPEKTEDGEGRAMITPALREALTKQGYQLIGSHSGVKLCRWTKVFIGFIQILFLFSATYLFLSIYFVCAHMCGGQSTAYRIWFSLWALGTKLSFSVTAATWMAPVWFSAVLLWYTSHAVRGSR

Target ORF information:

RefSeq Version	NM_178897.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus tRNA-yW synthesizing protein 1 homolog (S. cerevisiae) (Tyw1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_178897.4

ORF Insert Sequence:

ATGGGTCCTC TGGATGTGTG GGACTTGTCG CCTTTGCTGT CACTGTGGAT GAACAGGTTC 
TACATATATA TGGGCTGTGC CCTGGGCCTT ACCCTTTGCA TCTGTGTCCA GATCATCAAG 
AAGCAGGTCA CTCGTTCCCA GGAGAAACGT GTCCCAGGAG CTCCTGATAG CTCCCTCTCC 
CCTCAGAAGA AGCAGACACA CGTTTCTGGA GTGAAGATTT TTTACGGATC TCAGACGGGC 
ACTGCCAAGG GATTTGCAGT GGTTCTTGCC AAAGCTGTCA CCTCTCTGGA CCTGCCTGTG 
GCCATTATTA ATCTGAAGGA ATATGATCCA GATGACAGTC TGATAGGAGA GATCACTAGT 
AAAACCGTCT GTGCCTTCCT GGTCGCTACG TACACGGACG GCTGCCCTAC AGAGAGTGCA 
GAGTGGTTCT GCAAGTGGTT AGAGGAATCA GCTAATGATT TCCGCTTTGG CAAAACTTAC 
CTGAAGGGAC TTCGCTATGC AGTGTTTGGC CTGGGAGACT CTGCCTACCG GAGCCACTTC 
AACAAGGTCA GCACGAACGT TGACAAATGG CTCTGGATGC TCGGTGCCCA GCGGGTGCTG 
ACCCGAGGGG AAGGCGACTG CAATGCAGTT CAGAGCAAAC ATGGCAGCAT CGAGGCCGAC 
TTCACAGCCT GGAAGACCAA GTTCATCTCC CGGCTGCAGG CCCTGCAGAG AGGGGAGAAA 
AAGGCTTGTG GCGGGAACTG CAAGAGAGGG AAGTGTGAGT CGGCCCAGCA TGGCCCAGGA 
GAGGCGCGGC CTCACCCGCA AGGCGAATTG CATCCTGGGG ATGCTGAGGA GGAAGAGCCC 
TGTGAGAGCA GCAGCGAGGA CGAGCTCGGG ACCCAGGATT ATCAGAGCCT GACTTCTGTG 
GTGGATGTTG AGGACCTGGG CAACATCATG AACCCCGTGA AGAGGGAAAA GAGAGAAAAG 
TCTCATCAGG ACGGGAAAGC TGCCATGCAG AGGAACCCAG AGAAGACTGA GGACGGCGAA 
GGAAGAGCTA TGATAACACC TGCTCTGCGG GAAGCCCTGA CTAAACAAGG GTACCAGCTG 
ATTGGCAGCC ACTCTGGTGT GAAGCTTTGC AGGTGGACAA AGGTATTTAT TGGTTTTATT 
CAAATATTAT TTCTATTTTC TGCAACTTAT TTATTTTTGT CCATTTATTT TGTGTGTGCG 
CACATGTGTG GCGGTCAGAG CACAGCCTAC AGGATTTGGT TCTCTTTGTG GGCTCTGGGG 
ACCAAACTGT CTTTTAGTGT GACAGCAGCC ACTTGGATGG CTCCGGTATG GTTCTCAGCA 
GTGCTGTTGT GGTATACTTC ACATGCAGTA CGTGGCTCAC GTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tyw1 ( XM_017320584.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017176073.1 Mus musculus tRNA-yW synthesizing protein 1 homolog (S. cerevisiae) (Tyw1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu81205
Clone ID Related Accession (Same CDS sequence)	XM_017320584.1 , XM_017320584.2
Accession Version	XM_017320584.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1590bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCGGTG CCCAGCGGGT GCTGACCCGA GGGGAAGGCG ACTGCAATGC AGTTCAGAGC 
AAACATGGCA GCATCGAGGC CGACTTCACA GCCTGGAAGA CCAAGTTCAT CTCCCGGCTG 
CAGGCCCTGC AGAGAGGGGA GAAAAAGGCT TGTGGCGGGA ACTGCAAGAG AGGGAAGTGT 
GAGTCGGCCC AGCATGGCCC AGGAGAGGCG CGGCCTCACC CGCAAGGCGA ATTGCATCCT 
GGGGATGCTG AGGAGGAAGA GCCCTGTGAG AGCAGCAGCG AGGACGAGCT CGGGACCCAG 
GATTATCAGA GCCTGACTTC TGTGGTGGAT GTTGAGGACC TGGGCAACAT CATGAACCCC 
GTGAAGAGGG AAAAGAGAGA AAAGTCTCAT CAGGACGGGA AAGCTGCCAT GCAGAGGAAC 
CCAGAGAAGA CTGAGGACGG CGAAGGAAGA GCTATGATAA CACCTGCTCT GCGGGAAGCC 
CTGACTAAAC AAGGGTACCA GCTGATTGGC AGCCACTCTG GTGTGAAGCT TTGCAGGTGG 
ACAAAGTCGA TGCTGCGGGG GAGAGGAGGT TGCTACAAAC ACACATTCTA CGGCATTGAG 
AGCCACCGCT GCATGGAAGC CACCCCAAGC TTGGCGTGTG CGAATAAATG TGTCTTCTGC 
TGGCGGCACC ATACCAATCC TGTGGGCACT GAGTGGCGGT GGAAGATGGA CCAGCCAGAA 
TTGATCTTGA AGGAAGCCAT TGAGAACCAT CAGAACATGA TTAAACAGTT CAAAGGCGTG 
CCAGGCCTCA AGGCGGAGCG CTTTGAAGAG GGGATGGAGG TCAAGCACTG TGCGCTGTCA 
CTCGTGGGTG AGCCGATCAT GTACCCAGAA ATCAACAGGC TTTTGAAGCT GCTCCACCAG 
CACGGCATCT CCAGCTTCCT TGTCACCAAT GCGCAGTTCC CTGAGGAGAT CAGGAAGCTC 
ACACCAGTTA CTCAGCTCTA CGTGAGCGTG GACGCCAGCA CCAGAGACGG CCTGAAGAAG 
ATCGACCGCC CGCTCTTCAA GGATTTCTGG CAGCGCTTCC TAGACAGTTT GAAAGCCTTA 
TCTGCAAAGC AACAACGAAC GGTCTACAGA CTGACGCTTG TGAAGTGTTG GAACGTGGAT 
GAACTCCAGG CCTACGCTGA GCTGGTGTCC TTGGGGAACC CCGACTTCAT CGAGGTCAAG 
GGTGTGACCT ACTGTGGTGA GAGTGCAGCC AGCAGCCTCA CCATGGCCAA TGTCCCTTGG 
CACGAGGAAG TGGTTCGGTT TGTCCGTGAG CTGGTCGATC TGCTTCCCGA CTATGAAGTT 
GCGTGTGAAC ACGAGCACTC CAACTGCCTC CTGATCGGAC ACAAGAAGTT TAAGATCGAT 
GGAGAATGGT GGACATGGAT CAATTACAGC CGCTTCCAAG AGCTCGTTCT GCAGTACGAA 
GAGAGTGGAG GCTCCAAGAC GTTCAGCTCG AGGGATTACA TGGCCAGAAC CCCACAGTGG 
GCACTGTTTG GTGCCAGGGA AAGAGGCTTT GATCCCAAGG ACACGAGATA CCAGAGGAAG 
AACAAAACGA AGGACATTTC CGGATGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017176073.1
CDS	624..2213
Translation	MLGAQRVLTRGEGDCNAVQSKHGSIEADFTAWKTKFISRLQALQRGEKKACGGNCKRGKCESAQHGPGEARPHPQGELHPGDAEEEEPCESSSEDELGTQDYQSLTSVVDVEDLGNIMNPVKREKREKSHQDGKAAMQRNPEKTEDGEGRAMITPALREALTKQGYQLIGSHSGVKLCRWTKSMLRGRGGCYKHTFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGTEWRWKMDQPELILKEAIENHQNMIKQFKGVPGLKAERFEEGMEVKHCALSLVGEPIMYPEINRLLKLLHQHGISSFLVTNAQFPEEIRKLTPVTQLYVSVDASTRDGLKKIDRPLFKDFWQRFLDSLKALSAKQQRTVYRLTLVKCWNVDELQAYAELVSLGNPDFIEVKGVTYCGESAASSLTMANVPWHEEVVRFVRELVDLLPDYEVACEHEHSNCLLIGHKKFKIDGEWWTWINYSRFQELVLQYEESGGSKTFSSRDYMARTPQWALFGARERGFDPKDTRYQRKNKTKDISGC

Target ORF information:

RefSeq Version	XM_017320584.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus tRNA-yW synthesizing protein 1 homolog (S. cerevisiae) (Tyw1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017320584.1

ORF Insert Sequence:

ATGCTCGGTG CCCAGCGGGT GCTGACCCGA GGGGAAGGCG ACTGCAATGC AGTTCAGAGC 
AAACATGGCA GCATCGAGGC CGACTTCACA GCCTGGAAGA CCAAGTTCAT CTCCCGGCTG 
CAGGCCCTGC AGAGAGGGGA GAAAAAGGCT TGTGGCGGGA ACTGCAAGAG AGGGAAGTGT 
GAGTCGGCCC AGCATGGCCC AGGAGAGGCG CGGCCTCACC CGCAAGGCGA ATTGCATCCT 
GGGGATGCTG AGGAGGAAGA GCCCTGTGAG AGCAGCAGCG AGGACGAGCT CGGGACCCAG 
GATTATCAGA GCCTGACTTC TGTGGTGGAT GTTGAGGACC TGGGCAACAT CATGAACCCC 
GTGAAGAGGG AAAAGAGAGA AAAGTCTCAT CAGGACGGGA AAGCTGCCAT GCAGAGGAAC 
CCAGAGAAGA CTGAGGACGG CGAAGGAAGA GCTATGATAA CACCTGCTCT GCGGGAAGCC 
CTGACTAAAC AAGGGTACCA GCTGATTGGC AGCCACTCTG GTGTGAAGCT TTGCAGGTGG 
ACAAAGTCGA TGCTGCGGGG GAGAGGAGGT TGCTACAAAC ACACATTCTA CGGCATTGAG 
AGCCACCGCT GCATGGAAGC CACCCCAAGC TTGGCGTGTG CGAATAAATG TGTCTTCTGC 
TGGCGGCACC ATACCAATCC TGTGGGCACT GAGTGGCGGT GGAAGATGGA CCAGCCAGAA 
TTGATCTTGA AGGAAGCCAT TGAGAACCAT CAGAACATGA TTAAACAGTT CAAAGGCGTG 
CCAGGCCTCA AGGCGGAGCG CTTTGAAGAG GGGATGGAGG TCAAGCACTG TGCGCTGTCA 
CTCGTGGGTG AGCCGATCAT GTACCCAGAA ATCAACAGGC TTTTGAAGCT GCTCCACCAG 
CACGGCATCT CCAGCTTCCT TGTCACCAAT GCGCAGTTCC CTGAGGAGAT CAGGAAGCTC 
ACACCAGTTA CTCAGCTCTA CGTGAGCGTG GACGCCAGCA CCAGAGACGG CCTGAAGAAG 
ATCGACCGCC CGCTCTTCAA GGATTTCTGG CAGCGCTTCC TAGACAGTTT GAAAGCCTTA 
TCTGCAAAGC AACAACGAAC GGTCTACAGA CTGACGCTTG TGAAGTGTTG GAACGTGGAT 
GAACTCCAGG CCTACGCTGA GCTGGTGTCC TTGGGGAACC CCGACTTCAT CGAGGTCAAG 
GGTGTGACCT ACTGTGGTGA GAGTGCAGCC AGCAGCCTCA CCATGGCCAA TGTCCCTTGG 
CACGAGGAAG TGGTTCGGTT TGTCCGTGAG CTGGTCGATC TGCTTCCCGA CTATGAAGTT 
GCGTGTGAAC ACGAGCACTC CAACTGCCTC CTGATCGGAC ACAAGAAGTT TAAGATCGAT 
GGAGAATGGT GGACATGGAT CAATTACAGC CGCTTCCAAG AGCTCGTTCT GCAGTACGAA 
GAGAGTGGAG GCTCCAAGAC GTTCAGCTCG AGGGATTACA TGGCCAGAAC CCCACAGTGG 
GCACTGTTTG GTGCCAGGGA AAGAGGCTTT GATCCCAAGG ACACGAGATA CCAGAGGAAG 
AACAAAACGA AGGACATTTC CGGATGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tyw1 ( NM_001015876.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001015876.1 Mus musculus tRNA-yW synthesizing protein 1 homolog (S. cerevisiae) (Tyw1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu12980
Clone ID Related Accession (Same CDS sequence)	XM_006504331.1 , NM_001015876.2 , XM_006504331.2 , XM_006504331.3
Accession Version	NM_001015876.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2166bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-10
Organism	Mus musculus(house mouse)
Product	S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase TYW1 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BB619468.1, BC068126.1, CD551326.1 and BY670442.1. On Aug 1, 2009 this sequence version replaced NM_001015876.1. Transcript Variant: This variant (1) encodes the longer isoform (1). ##Evidence-Data-START## Transcript exon combination :: BC068126.1, AK082884.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGTCCTC TGGATGTGTG GGACTTGTCG CCTTTGCTGT CACTGTGGAT GAACAGGTTC 
TACATATATA TGGGCTGTGC CCTGGGCCTT ACCCTTTGCA TCTGTGTCCA GATCATCAAG 
AAGCAGGTCA CTCGTTCCCA GGAGAAACGT GTCCCAGGAG CTCCTGATAG CTCCCTCTCC 
CCTCAGAAGA AGCAGACACA CGTTTCTGGA GTGAAGATTT TTTACGGATC TCAGACGGGC 
ACTGCCAAGG GATTTGCAGT GGTTCTTGCC AAAGCTGTCA CCTCTCTGGA CCTGCCTGTG 
GCCATTATTA ATCTGAAGGA ATATGATCCA GATGACAGTC TGATAGGAGA GATCACTAGT 
AAAACCGTCT GTGCCTTCCT GGTCGCTACG TACACGGACG GCTGCCCTAC AGAGAGTGCA 
GAGTGGTTCT GCAAGTGGTT AGAGGAATCA GCTAATGATT TCCGCTTTGG CAAAACTTAC 
CTGAAGGGAC TTCGCTATGC AGTGTTTGGC CTGGGAGACT CTGCCTACCG GAGCCACTTC 
AACAAGGTCA GCACGAACGT TGACAAATGG CTCTGGATGC TCGGTGCCCA GCGGGTGCTG 
ACCCGAGGGG AAGGCGACTG CAATGCAGTT CAGAGCAAAC ATGGCAGCAT CGAGGCCGAC 
TTCACAGCCT GGAAGACCAA GTTCATCTCC CGGCTGCAGG CCCTGCAGAG AGGGGAGAAA 
AAGGCTTGTG GCGGGAACTG CAAGAGAGGG AAGTGTGAGT CGGCCCAGCA TGGCCCAGGA 
GAGGCGCGGC CTCACCCGCA AGGCGAATTG CATCCTGGGG ATGCTGAGGA GGAAGAGCCC 
TGTGAGAGCA GCAGCGAGGA CGAGCTCGGG ACCCAGGATT ATCAGAGCCT GACTTCTGTG 
GTGGATGTTG AGGACCTGGG CAACATCATG AACCCCGTGA AGAGGGAAAA GAGAGAAAAG 
TCTCATCAGG ACGGGAAAGC TGCCATGCAG AGGAACCCAG AGAAGACTGA GGACGGCGAA 
GGAAGAGCTA TGATAACACC TGCTCTGCGG GAAGCCCTGA CTAAACAAGG GTACCAGCTG 
ATTGGCAGCC ACTCTGGTGT GAAGCTTTGC AGGTGGACAA AGTCGATGCT GCGGGGGAGA 
GGAGGTTGCT ACAAACACAC ATTCTACGGC ATTGAGAGCC ACCGCTGCAT GGAAGCCACC 
CCAAGCTTGG CGTGTGCGAA TAAATGTGTC TTCTGCTGGC GGCACCATAC CAATCCTGTG 
GGCACTGAGT GGCGGTGGAA GATGGACCAG CCAGAATTGA TCTTGAAGGA AGCCATTGAG 
AACCATCAGA ACATGATTAA ACAGTTCAAA GGCGTGCCAG GCCTCAAGGC GGAGCGCTTT 
GAAGAGGGGA TGGAGGTCAA GCACTGTGCG CTGTCACTCG TGGGTGAGCC GATCATGTAC 
CCAGAAATCA ACAGGCTTTT GAAGCTGCTC CACCAGCACG GCATCTCCAG CTTCCTTGTC 
ACCAATGCGC AGTTCCCTGA GGAGATCAGG AAGCTCACAC CAGTTACTCA GCTCTACGTG 
AGCGTGGACG CCAGCACCAG AGACGGCCTG AAGAAGATCG ACCGCCCGCT CTTCAAGGAT 
TTCTGGCAGC GCTTCCTAGA CAGTTTGAAA GCCTTATCTG CAAAGCAACA ACGAACGGTC 
TACAGACTGA CGCTTGTGAA GTGTTGGAAC GTGGATGAAC TCCAGGCCTA CGCTGAGCTG 
GTGTCCTTGG GGAACCCCGA CTTCATCGAG GTCAAGGGTG TGACCTACTG TGGTGAGAGT 
GCAGCCAGCA GCCTCACCAT GGCCAATGTC CCTTGGCACG AGGAAGTGGT TCGGTTTGTC 
CGTGAGCTGG TCGATCTGCT TCCCGACTAT GAAGTTGCGT GTGAACACGA GCACTCCAAC 
TGCCTCCTGA TCGGACACAA GAAGTTTAAG ATCGATGGAG AATGGTGGAC ATGGATCAAT 
TACAGCCGCT TCCAAGAGCT CGTTCTGCAG TACGAAGAGA GTGGAGGCTC CAAGACGTTC 
AGCTCGAGGG ATTACATGGC CAGAACCCCA CAGTGGGCAC TGTTTGGTGC CAGGGAAAGA 
GGCTTTGATC CCAAGGACAC GAGATACCAG AGGAAGAACA AAACGAAGGA CATTTCCGGA 
TGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001015876.1
CDS	154..2319
Translation	MGPLDVWDLSPLLSLWMNRFYIYMGCALGLTLCICVQIIKKQVTRSQEKRVPGAPDSSLSPQKKQTHVSGVKIFYGSQTGTAKGFAVVLAKAVTSLDLPVAIINLKEYDPDDSLIGEITSKTVCAFLVATYTDGCPTESAEWFCKWLEESANDFRFGKTYLKGLRYAVFGLGDSAYRSHFNKVSTNVDKWLWMLGAQRVLTRGEGDCNAVQSKHGSIEADFTAWKTKFISRLQALQRGEKKACGGNCKRGKCESAQHGPGEARPHPQGELHPGDAEEEEPCESSSEDELGTQDYQSLTSVVDVEDLGNIMNPVKREKREKSHQDGKAAMQRNPEKTEDGEGRAMITPALREALTKQGYQLIGSHSGVKLCRWTKSMLRGRGGCYKHTFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGTEWRWKMDQPELILKEAIENHQNMIKQFKGVPGLKAERFEEGMEVKHCALSLVGEPIMYPEINRLLKLLHQHGISSFLVTNAQFPEEIRKLTPVTQLYVSVDASTRDGLKKIDRPLFKDFWQRFLDSLKALSAKQQRTVYRLTLVKCWNVDELQAYAELVSLGNPDFIEVKGVTYCGESAASSLTMANVPWHEEVVRFVRELVDLLPDYEVACEHEHSNCLLIGHKKFKIDGEWWTWINYSRFQELVLQYEESGGSKTFSSRDYMARTPQWALFGARERGFDPKDTRYQRKNKTKDISGC

Target ORF information:

RefSeq Version	NM_001015876.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus tRNA-yW synthesizing protein 1 homolog (S. cerevisiae) (Tyw1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001015876.2

ORF Insert Sequence:

ATGGGTCCTC TGGATGTGTG GGACTTGTCG CCTTTGCTGT CACTGTGGAT GAACAGGTTC 
TACATATATA TGGGCTGTGC CCTGGGCCTT ACCCTTTGCA TCTGTGTCCA GATCATCAAG 
AAGCAGGTCA CTCGTTCCCA GGAGAAACGT GTCCCAGGAG CTCCTGATAG CTCCCTCTCC 
CCTCAGAAGA AGCAGACACA CGTTTCTGGA GTGAAGATTT TTTACGGATC TCAGACGGGC 
ACTGCCAAGG GATTTGCAGT GGTTCTTGCC AAAGCTGTCA CCTCTCTGGA CCTGCCTGTG 
GCCATTATTA ATCTGAAGGA ATATGATCCA GATGACAGTC TGATAGGAGA GATCACTAGT 
AAAACCGTCT GTGCCTTCCT GGTCGCTACG TACACGGACG GCTGCCCTAC AGAGAGTGCA 
GAGTGGTTCT GCAAGTGGTT AGAGGAATCA GCTAATGATT TCCGCTTTGG CAAAACTTAC 
CTGAAGGGAC TTCGCTATGC AGTGTTTGGC CTGGGAGACT CTGCCTACCG GAGCCACTTC 
AACAAGGTCA GCACGAACGT TGACAAATGG CTCTGGATGC TCGGTGCCCA GCGGGTGCTG 
ACCCGAGGGG AAGGCGACTG CAATGCAGTT CAGAGCAAAC ATGGCAGCAT CGAGGCCGAC 
TTCACAGCCT GGAAGACCAA GTTCATCTCC CGGCTGCAGG CCCTGCAGAG AGGGGAGAAA 
AAGGCTTGTG GCGGGAACTG CAAGAGAGGG AAGTGTGAGT CGGCCCAGCA TGGCCCAGGA 
GAGGCGCGGC CTCACCCGCA AGGCGAATTG CATCCTGGGG ATGCTGAGGA GGAAGAGCCC 
TGTGAGAGCA GCAGCGAGGA CGAGCTCGGG ACCCAGGATT ATCAGAGCCT GACTTCTGTG 
GTGGATGTTG AGGACCTGGG CAACATCATG AACCCCGTGA AGAGGGAAAA GAGAGAAAAG 
TCTCATCAGG ACGGGAAAGC TGCCATGCAG AGGAACCCAG AGAAGACTGA GGACGGCGAA 
GGAAGAGCTA TGATAACACC TGCTCTGCGG GAAGCCCTGA CTAAACAAGG GTACCAGCTG 
ATTGGCAGCC ACTCTGGTGT GAAGCTTTGC AGGTGGACAA AGTCGATGCT GCGGGGGAGA 
GGAGGTTGCT ACAAACACAC ATTCTACGGC ATTGAGAGCC ACCGCTGCAT GGAAGCCACC 
CCAAGCTTGG CGTGTGCGAA TAAATGTGTC TTCTGCTGGC GGCACCATAC CAATCCTGTG 
GGCACTGAGT GGCGGTGGAA GATGGACCAG CCAGAATTGA TCTTGAAGGA AGCCATTGAG 
AACCATCAGA ACATGATTAA ACAGTTCAAA GGCGTGCCAG GCCTCAAGGC GGAGCGCTTT 
GAAGAGGGGA TGGAGGTCAA GCACTGTGCG CTGTCACTCG TGGGTGAGCC GATCATGTAC 
CCAGAAATCA ACAGGCTTTT GAAGCTGCTC CACCAGCACG GCATCTCCAG CTTCCTTGTC 
ACCAATGCGC AGTTCCCTGA GGAGATCAGG AAGCTCACAC CAGTTACTCA GCTCTACGTG 
AGCGTGGACG CCAGCACCAG AGACGGCCTG AAGAAGATCG ACCGCCCGCT CTTCAAGGAT 
TTCTGGCAGC GCTTCCTAGA CAGTTTGAAA GCCTTATCTG CAAAGCAACA ACGAACGGTC 
TACAGACTGA CGCTTGTGAA GTGTTGGAAC GTGGATGAAC TCCAGGCCTA CGCTGAGCTG 
GTGTCCTTGG GGAACCCCGA CTTCATCGAG GTCAAGGGTG TGACCTACTG TGGTGAGAGT 
GCAGCCAGCA GCCTCACCAT GGCCAATGTC CCTTGGCACG AGGAAGTGGT TCGGTTTGTC 
CGTGAGCTGG TCGATCTGCT TCCCGACTAT GAAGTTGCGT GTGAACACGA GCACTCCAAC 
TGCCTCCTGA TCGGACACAA GAAGTTTAAG ATCGATGGAG AATGGTGGAC ATGGATCAAT 
TACAGCCGCT TCCAAGAGCT CGTTCTGCAG TACGAAGAGA GTGGAGGCTC CAAGACGTTC 
AGCTCGAGGG ATTACATGGC CAGAACCCCA CAGTGGGCAC TGTTTGGTGC CAGGGAAAGA 
GGCTTTGATC CCAAGGACAC GAGATACCAG AGGAAGAACA AAACGAAGGA CATTTCCGGA 
TGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tyw1 ( XM_006504331.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006504394.1 Mus musculus tRNA-yW synthesizing protein 1 homolog (S. cerevisiae) (Tyw1), transcript variant X1, mRNA.

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CloneID	OMu12980
Clone ID Related Accession (Same CDS sequence)	XM_006504331.1 , NM_001015876.2 , XM_006504331.2 , XM_006504331.3
Accession Version	XM_006504331.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2166bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-02-08
Organism	Mus musculus(House mouse)
Product	S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase isoform X1
Comment

ATGGGTCCTC TGGATGTGTG GGACTTGTCG CCTTTGCTGT CACTGTGGAT GAACAGGTTC 
TACATATATA TGGGCTGTGC CCTGGGCCTT ACCCTTTGCA TCTGTGTCCA GATCATCAAG 
AAGCAGGTCA CTCGTTCCCA GGAGAAACGT GTCCCAGGAG CTCCTGATAG CTCCCTCTCC 
CCTCAGAAGA AGCAGACACA CGTTTCTGGA GTGAAGATTT TTTACGGATC TCAGACGGGC 
ACTGCCAAGG GATTTGCAGT GGTTCTTGCC AAAGCTGTCA CCTCTCTGGA CCTGCCTGTG 
GCCATTATTA ATCTGAAGGA ATATGATCCA GATGACAGTC TGATAGGAGA GATCACTAGT 
AAAACCGTCT GTGCCTTCCT GGTCGCTACG TACACGGACG GCTGCCCTAC AGAGAGTGCA 
GAGTGGTTCT GCAAGTGGTT AGAGGAATCA GCTAATGATT TCCGCTTTGG CAAAACTTAC 
CTGAAGGGAC TTCGCTATGC AGTGTTTGGC CTGGGAGACT CTGCCTACCG GAGCCACTTC 
AACAAGGTCA GCACGAACGT TGACAAATGG CTCTGGATGC TCGGTGCCCA GCGGGTGCTG 
ACCCGAGGGG AAGGCGACTG CAATGCAGTT CAGAGCAAAC ATGGCAGCAT CGAGGCCGAC 
TTCACAGCCT GGAAGACCAA GTTCATCTCC CGGCTGCAGG CCCTGCAGAG AGGGGAGAAA 
AAGGCTTGTG GCGGGAACTG CAAGAGAGGG AAGTGTGAGT CGGCCCAGCA TGGCCCAGGA 
GAGGCGCGGC CTCACCCGCA AGGCGAATTG CATCCTGGGG ATGCTGAGGA GGAAGAGCCC 
TGTGAGAGCA GCAGCGAGGA CGAGCTCGGG ACCCAGGATT ATCAGAGCCT GACTTCTGTG 
GTGGATGTTG AGGACCTGGG CAACATCATG AACCCCGTGA AGAGGGAAAA GAGAGAAAAG 
TCTCATCAGG ACGGGAAAGC TGCCATGCAG AGGAACCCAG AGAAGACTGA GGACGGCGAA 
GGAAGAGCTA TGATAACACC TGCTCTGCGG GAAGCCCTGA CTAAACAAGG GTACCAGCTG 
ATTGGCAGCC ACTCTGGTGT GAAGCTTTGC AGGTGGACAA AGTCGATGCT GCGGGGGAGA 
GGAGGTTGCT ACAAACACAC ATTCTACGGC ATTGAGAGCC ACCGCTGCAT GGAAGCCACC 
CCAAGCTTGG CGTGTGCGAA TAAATGTGTC TTCTGCTGGC GGCACCATAC CAATCCTGTG 
GGCACTGAGT GGCGGTGGAA GATGGACCAG CCAGAATTGA TCTTGAAGGA AGCCATTGAG 
AACCATCAGA ACATGATTAA ACAGTTCAAA GGCGTGCCAG GCCTCAAGGC GGAGCGCTTT 
GAAGAGGGGA TGGAGGTCAA GCACTGTGCG CTGTCACTCG TGGGTGAGCC GATCATGTAC 
CCAGAAATCA ACAGGCTTTT GAAGCTGCTC CACCAGCACG GCATCTCCAG CTTCCTTGTC 
ACCAATGCGC AGTTCCCTGA GGAGATCAGG AAGCTCACAC CAGTTACTCA GCTCTACGTG 
AGCGTGGACG CCAGCACCAG AGACGGCCTG AAGAAGATCG ACCGCCCGCT CTTCAAGGAT 
TTCTGGCAGC GCTTCCTAGA CAGTTTGAAA GCCTTATCTG CAAAGCAACA ACGAACGGTC 
TACAGACTGA CGCTTGTGAA GTGTTGGAAC GTGGATGAAC TCCAGGCCTA CGCTGAGCTG 
GTGTCCTTGG GGAACCCCGA CTTCATCGAG GTCAAGGGTG TGACCTACTG TGGTGAGAGT 
GCAGCCAGCA GCCTCACCAT GGCCAATGTC CCTTGGCACG AGGAAGTGGT TCGGTTTGTC 
CGTGAGCTGG TCGATCTGCT TCCCGACTAT GAAGTTGCGT GTGAACACGA GCACTCCAAC 
TGCCTCCTGA TCGGACACAA GAAGTTTAAG ATCGATGGAG AATGGTGGAC ATGGATCAAT 
TACAGCCGCT TCCAAGAGCT CGTTCTGCAG TACGAAGAGA GTGGAGGCTC CAAGACGTTC 
AGCTCGAGGG ATTACATGGC CAGAACCCCA CAGTGGGCAC TGTTTGGTGC CAGGGAAAGA 
GGCTTTGATC CCAAGGACAC GAGATACCAG AGGAAGAACA AAACGAAGGA CATTTCCGGA 
TGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

XP_006504394.1

Translation

MGPLDVWDLSPLLSLWMNRFYIYMGCALGLTLCICVQIIKKQVTRSQEKRVPGAPDSSLSPQKKQTHVSGVKIFYGSQTGTAKGFAVVLAKAVTSLDLPVAIINLKEYDPDDSLIGEITSKTVCAFLVATYTDGCPTESAEWFCKWLEESANDFRFGKTYLKGLRYAVFGLGDSAYRSHFNKVSTNVDKWLWMLGAQRVLTRGEGDCNAVQSKHGSIEADFTAWKTKFISRLQALQRGEKKACGGNCKRGKCESAQHGPGEARPHPQGELHPGDAEEEEPCESSSEDELGTQDYQSLTSVVDVEDLGNIMNPVKREKREKSHQDGKAAMQRNPEKTEDGEGRAMITPALREALTKQGYQLIGSHSGVKLCRWTKSMLRGRGGCYKHTFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGTEWRWKMDQPELILKEAIENHQNMIKQFKGVPGLKAERFEEGMEVKHCALSLVGEPIMYPEINRLLKLLHQHGISSFLVTNAQFPEEIRKLTPVTQLYVSVDASTRDGLKKIDRPLFKDFWQRFLDSLKALSAKQQRTVYRLTLVKCWNVDELQAYAELVSLGNPDFIEVKGVTYCGESAASSLTMANVPWHEEVVRFVRELVDLLPDYEVACEHEHSNCLLIGHKKFKIDGEWWTWINYSRFQELVLQYEESGGSKTFSSRDYMARTPQWALFGARERGFDPKDTRYQRKNKTKDISGC

Target ORF information:

RefSeq Version	XM_006504331.1
Organism	Mus musculus(House mouse)
Definition	Mus musculus tRNA-yW synthesizing protein 1 homolog (S. cerevisiae) (Tyw1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006504331.1

ORF Insert Sequence:

ATGGGTCCTC TGGATGTGTG GGACTTGTCG CCTTTGCTGT CACTGTGGAT GAACAGGTTC 
TACATATATA TGGGCTGTGC CCTGGGCCTT ACCCTTTGCA TCTGTGTCCA GATCATCAAG 
AAGCAGGTCA CTCGTTCCCA GGAGAAACGT GTCCCAGGAG CTCCTGATAG CTCCCTCTCC 
CCTCAGAAGA AGCAGACACA CGTTTCTGGA GTGAAGATTT TTTACGGATC TCAGACGGGC 
ACTGCCAAGG GATTTGCAGT GGTTCTTGCC AAAGCTGTCA CCTCTCTGGA CCTGCCTGTG 
GCCATTATTA ATCTGAAGGA ATATGATCCA GATGACAGTC TGATAGGAGA GATCACTAGT 
AAAACCGTCT GTGCCTTCCT GGTCGCTACG TACACGGACG GCTGCCCTAC AGAGAGTGCA 
GAGTGGTTCT GCAAGTGGTT AGAGGAATCA GCTAATGATT TCCGCTTTGG CAAAACTTAC 
CTGAAGGGAC TTCGCTATGC AGTGTTTGGC CTGGGAGACT CTGCCTACCG GAGCCACTTC 
AACAAGGTCA GCACGAACGT TGACAAATGG CTCTGGATGC TCGGTGCCCA GCGGGTGCTG 
ACCCGAGGGG AAGGCGACTG CAATGCAGTT CAGAGCAAAC ATGGCAGCAT CGAGGCCGAC 
TTCACAGCCT GGAAGACCAA GTTCATCTCC CGGCTGCAGG CCCTGCAGAG AGGGGAGAAA 
AAGGCTTGTG GCGGGAACTG CAAGAGAGGG AAGTGTGAGT CGGCCCAGCA TGGCCCAGGA 
GAGGCGCGGC CTCACCCGCA AGGCGAATTG CATCCTGGGG ATGCTGAGGA GGAAGAGCCC 
TGTGAGAGCA GCAGCGAGGA CGAGCTCGGG ACCCAGGATT ATCAGAGCCT GACTTCTGTG 
GTGGATGTTG AGGACCTGGG CAACATCATG AACCCCGTGA AGAGGGAAAA GAGAGAAAAG 
TCTCATCAGG ACGGGAAAGC TGCCATGCAG AGGAACCCAG AGAAGACTGA GGACGGCGAA 
GGAAGAGCTA TGATAACACC TGCTCTGCGG GAAGCCCTGA CTAAACAAGG GTACCAGCTG 
ATTGGCAGCC ACTCTGGTGT GAAGCTTTGC AGGTGGACAA AGTCGATGCT GCGGGGGAGA 
GGAGGTTGCT ACAAACACAC ATTCTACGGC ATTGAGAGCC ACCGCTGCAT GGAAGCCACC 
CCAAGCTTGG CGTGTGCGAA TAAATGTGTC TTCTGCTGGC GGCACCATAC CAATCCTGTG 
GGCACTGAGT GGCGGTGGAA GATGGACCAG CCAGAATTGA TCTTGAAGGA AGCCATTGAG 
AACCATCAGA ACATGATTAA ACAGTTCAAA GGCGTGCCAG GCCTCAAGGC GGAGCGCTTT 
GAAGAGGGGA TGGAGGTCAA GCACTGTGCG CTGTCACTCG TGGGTGAGCC GATCATGTAC 
CCAGAAATCA ACAGGCTTTT GAAGCTGCTC CACCAGCACG GCATCTCCAG CTTCCTTGTC 
ACCAATGCGC AGTTCCCTGA GGAGATCAGG AAGCTCACAC CAGTTACTCA GCTCTACGTG 
AGCGTGGACG CCAGCACCAG AGACGGCCTG AAGAAGATCG ACCGCCCGCT CTTCAAGGAT 
TTCTGGCAGC GCTTCCTAGA CAGTTTGAAA GCCTTATCTG CAAAGCAACA ACGAACGGTC 
TACAGACTGA CGCTTGTGAA GTGTTGGAAC GTGGATGAAC TCCAGGCCTA CGCTGAGCTG 
GTGTCCTTGG GGAACCCCGA CTTCATCGAG GTCAAGGGTG TGACCTACTG TGGTGAGAGT 
GCAGCCAGCA GCCTCACCAT GGCCAATGTC CCTTGGCACG AGGAAGTGGT TCGGTTTGTC 
CGTGAGCTGG TCGATCTGCT TCCCGACTAT GAAGTTGCGT GTGAACACGA GCACTCCAAC 
TGCCTCCTGA TCGGACACAA GAAGTTTAAG ATCGATGGAG AATGGTGGAC ATGGATCAAT 
TACAGCCGCT TCCAAGAGCT CGTTCTGCAG TACGAAGAGA GTGGAGGCTC CAAGACGTTC 
AGCTCGAGGG ATTACATGGC CAGAACCCCA CAGTGGGCAC TGTTTGGTGC CAGGGAAAGA 
GGCTTTGATC CCAAGGACAC GAGATACCAG AGGAAGAACA AAACGAAGGA CATTTCCGGA 
TGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tyw1 ( XM_006504331.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006504394.1 Mus musculus tRNA-yW synthesizing protein 1 homolog (S. cerevisiae) (Tyw1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu12980
Clone ID Related Accession (Same CDS sequence)	XM_006504331.1 , NM_001015876.2 , XM_006504331.2 , XM_006504331.3
Accession Version	XM_006504331.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2166bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006504331.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTCCTC TGGATGTGTG GGACTTGTCG CCTTTGCTGT CACTGTGGAT GAACAGGTTC 
TACATATATA TGGGCTGTGC CCTGGGCCTT ACCCTTTGCA TCTGTGTCCA GATCATCAAG 
AAGCAGGTCA CTCGTTCCCA GGAGAAACGT GTCCCAGGAG CTCCTGATAG CTCCCTCTCC 
CCTCAGAAGA AGCAGACACA CGTTTCTGGA GTGAAGATTT TTTACGGATC TCAGACGGGC 
ACTGCCAAGG GATTTGCAGT GGTTCTTGCC AAAGCTGTCA CCTCTCTGGA CCTGCCTGTG 
GCCATTATTA ATCTGAAGGA ATATGATCCA GATGACAGTC TGATAGGAGA GATCACTAGT 
AAAACCGTCT GTGCCTTCCT GGTCGCTACG TACACGGACG GCTGCCCTAC AGAGAGTGCA 
GAGTGGTTCT GCAAGTGGTT AGAGGAATCA GCTAATGATT TCCGCTTTGG CAAAACTTAC 
CTGAAGGGAC TTCGCTATGC AGTGTTTGGC CTGGGAGACT CTGCCTACCG GAGCCACTTC 
AACAAGGTCA GCACGAACGT TGACAAATGG CTCTGGATGC TCGGTGCCCA GCGGGTGCTG 
ACCCGAGGGG AAGGCGACTG CAATGCAGTT CAGAGCAAAC ATGGCAGCAT CGAGGCCGAC 
TTCACAGCCT GGAAGACCAA GTTCATCTCC CGGCTGCAGG CCCTGCAGAG AGGGGAGAAA 
AAGGCTTGTG GCGGGAACTG CAAGAGAGGG AAGTGTGAGT CGGCCCAGCA TGGCCCAGGA 
GAGGCGCGGC CTCACCCGCA AGGCGAATTG CATCCTGGGG ATGCTGAGGA GGAAGAGCCC 
TGTGAGAGCA GCAGCGAGGA CGAGCTCGGG ACCCAGGATT ATCAGAGCCT GACTTCTGTG 
GTGGATGTTG AGGACCTGGG CAACATCATG AACCCCGTGA AGAGGGAAAA GAGAGAAAAG 
TCTCATCAGG ACGGGAAAGC TGCCATGCAG AGGAACCCAG AGAAGACTGA GGACGGCGAA 
GGAAGAGCTA TGATAACACC TGCTCTGCGG GAAGCCCTGA CTAAACAAGG GTACCAGCTG 
ATTGGCAGCC ACTCTGGTGT GAAGCTTTGC AGGTGGACAA AGTCGATGCT GCGGGGGAGA 
GGAGGTTGCT ACAAACACAC ATTCTACGGC ATTGAGAGCC ACCGCTGCAT GGAAGCCACC 
CCAAGCTTGG CGTGTGCGAA TAAATGTGTC TTCTGCTGGC GGCACCATAC CAATCCTGTG 
GGCACTGAGT GGCGGTGGAA GATGGACCAG CCAGAATTGA TCTTGAAGGA AGCCATTGAG 
AACCATCAGA ACATGATTAA ACAGTTCAAA GGCGTGCCAG GCCTCAAGGC GGAGCGCTTT 
GAAGAGGGGA TGGAGGTCAA GCACTGTGCG CTGTCACTCG TGGGTGAGCC GATCATGTAC 
CCAGAAATCA ACAGGCTTTT GAAGCTGCTC CACCAGCACG GCATCTCCAG CTTCCTTGTC 
ACCAATGCGC AGTTCCCTGA GGAGATCAGG AAGCTCACAC CAGTTACTCA GCTCTACGTG 
AGCGTGGACG CCAGCACCAG AGACGGCCTG AAGAAGATCG ACCGCCCGCT CTTCAAGGAT 
TTCTGGCAGC GCTTCCTAGA CAGTTTGAAA GCCTTATCTG CAAAGCAACA ACGAACGGTC 
TACAGACTGA CGCTTGTGAA GTGTTGGAAC GTGGATGAAC TCCAGGCCTA CGCTGAGCTG 
GTGTCCTTGG GGAACCCCGA CTTCATCGAG GTCAAGGGTG TGACCTACTG TGGTGAGAGT 
GCAGCCAGCA GCCTCACCAT GGCCAATGTC CCTTGGCACG AGGAAGTGGT TCGGTTTGTC 
CGTGAGCTGG TCGATCTGCT TCCCGACTAT GAAGTTGCGT GTGAACACGA GCACTCCAAC 
TGCCTCCTGA TCGGACACAA GAAGTTTAAG ATCGATGGAG AATGGTGGAC ATGGATCAAT 
TACAGCCGCT TCCAAGAGCT CGTTCTGCAG TACGAAGAGA GTGGAGGCTC CAAGACGTTC 
AGCTCGAGGG ATTACATGGC CAGAACCCCA CAGTGGGCAC TGTTTGGTGC CAGGGAAAGA 
GGCTTTGATC CCAAGGACAC GAGATACCAG AGGAAGAACA AAACGAAGGA CATTTCCGGA 
TGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006504394.1
CDS	71..2236
Translation	MGPLDVWDLSPLLSLWMNRFYIYMGCALGLTLCICVQIIKKQVTRSQEKRVPGAPDSSLSPQKKQTHVSGVKIFYGSQTGTAKGFAVVLAKAVTSLDLPVAIINLKEYDPDDSLIGEITSKTVCAFLVATYTDGCPTESAEWFCKWLEESANDFRFGKTYLKGLRYAVFGLGDSAYRSHFNKVSTNVDKWLWMLGAQRVLTRGEGDCNAVQSKHGSIEADFTAWKTKFISRLQALQRGEKKACGGNCKRGKCESAQHGPGEARPHPQGELHPGDAEEEEPCESSSEDELGTQDYQSLTSVVDVEDLGNIMNPVKREKREKSHQDGKAAMQRNPEKTEDGEGRAMITPALREALTKQGYQLIGSHSGVKLCRWTKSMLRGRGGCYKHTFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGTEWRWKMDQPELILKEAIENHQNMIKQFKGVPGLKAERFEEGMEVKHCALSLVGEPIMYPEINRLLKLLHQHGISSFLVTNAQFPEEIRKLTPVTQLYVSVDASTRDGLKKIDRPLFKDFWQRFLDSLKALSAKQQRTVYRLTLVKCWNVDELQAYAELVSLGNPDFIEVKGVTYCGESAASSLTMANVPWHEEVVRFVRELVDLLPDYEVACEHEHSNCLLIGHKKFKIDGEWWTWINYSRFQELVLQYEESGGSKTFSSRDYMARTPQWALFGARERGFDPKDTRYQRKNKTKDISGC

Target ORF information:

RefSeq Version	XM_006504331.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus tRNA-yW synthesizing protein 1 homolog (S. cerevisiae) (Tyw1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006504331.2

ORF Insert Sequence:

ATGGGTCCTC TGGATGTGTG GGACTTGTCG CCTTTGCTGT CACTGTGGAT GAACAGGTTC 
TACATATATA TGGGCTGTGC CCTGGGCCTT ACCCTTTGCA TCTGTGTCCA GATCATCAAG 
AAGCAGGTCA CTCGTTCCCA GGAGAAACGT GTCCCAGGAG CTCCTGATAG CTCCCTCTCC 
CCTCAGAAGA AGCAGACACA CGTTTCTGGA GTGAAGATTT TTTACGGATC TCAGACGGGC 
ACTGCCAAGG GATTTGCAGT GGTTCTTGCC AAAGCTGTCA CCTCTCTGGA CCTGCCTGTG 
GCCATTATTA ATCTGAAGGA ATATGATCCA GATGACAGTC TGATAGGAGA GATCACTAGT 
AAAACCGTCT GTGCCTTCCT GGTCGCTACG TACACGGACG GCTGCCCTAC AGAGAGTGCA 
GAGTGGTTCT GCAAGTGGTT AGAGGAATCA GCTAATGATT TCCGCTTTGG CAAAACTTAC 
CTGAAGGGAC TTCGCTATGC AGTGTTTGGC CTGGGAGACT CTGCCTACCG GAGCCACTTC 
AACAAGGTCA GCACGAACGT TGACAAATGG CTCTGGATGC TCGGTGCCCA GCGGGTGCTG 
ACCCGAGGGG AAGGCGACTG CAATGCAGTT CAGAGCAAAC ATGGCAGCAT CGAGGCCGAC 
TTCACAGCCT GGAAGACCAA GTTCATCTCC CGGCTGCAGG CCCTGCAGAG AGGGGAGAAA 
AAGGCTTGTG GCGGGAACTG CAAGAGAGGG AAGTGTGAGT CGGCCCAGCA TGGCCCAGGA 
GAGGCGCGGC CTCACCCGCA AGGCGAATTG CATCCTGGGG ATGCTGAGGA GGAAGAGCCC 
TGTGAGAGCA GCAGCGAGGA CGAGCTCGGG ACCCAGGATT ATCAGAGCCT GACTTCTGTG 
GTGGATGTTG AGGACCTGGG CAACATCATG AACCCCGTGA AGAGGGAAAA GAGAGAAAAG 
TCTCATCAGG ACGGGAAAGC TGCCATGCAG AGGAACCCAG AGAAGACTGA GGACGGCGAA 
GGAAGAGCTA TGATAACACC TGCTCTGCGG GAAGCCCTGA CTAAACAAGG GTACCAGCTG 
ATTGGCAGCC ACTCTGGTGT GAAGCTTTGC AGGTGGACAA AGTCGATGCT GCGGGGGAGA 
GGAGGTTGCT ACAAACACAC ATTCTACGGC ATTGAGAGCC ACCGCTGCAT GGAAGCCACC 
CCAAGCTTGG CGTGTGCGAA TAAATGTGTC TTCTGCTGGC GGCACCATAC CAATCCTGTG 
GGCACTGAGT GGCGGTGGAA GATGGACCAG CCAGAATTGA TCTTGAAGGA AGCCATTGAG 
AACCATCAGA ACATGATTAA ACAGTTCAAA GGCGTGCCAG GCCTCAAGGC GGAGCGCTTT 
GAAGAGGGGA TGGAGGTCAA GCACTGTGCG CTGTCACTCG TGGGTGAGCC GATCATGTAC 
CCAGAAATCA ACAGGCTTTT GAAGCTGCTC CACCAGCACG GCATCTCCAG CTTCCTTGTC 
ACCAATGCGC AGTTCCCTGA GGAGATCAGG AAGCTCACAC CAGTTACTCA GCTCTACGTG 
AGCGTGGACG CCAGCACCAG AGACGGCCTG AAGAAGATCG ACCGCCCGCT CTTCAAGGAT 
TTCTGGCAGC GCTTCCTAGA CAGTTTGAAA GCCTTATCTG CAAAGCAACA ACGAACGGTC 
TACAGACTGA CGCTTGTGAA GTGTTGGAAC GTGGATGAAC TCCAGGCCTA CGCTGAGCTG 
GTGTCCTTGG GGAACCCCGA CTTCATCGAG GTCAAGGGTG TGACCTACTG TGGTGAGAGT 
GCAGCCAGCA GCCTCACCAT GGCCAATGTC CCTTGGCACG AGGAAGTGGT TCGGTTTGTC 
CGTGAGCTGG TCGATCTGCT TCCCGACTAT GAAGTTGCGT GTGAACACGA GCACTCCAAC 
TGCCTCCTGA TCGGACACAA GAAGTTTAAG ATCGATGGAG AATGGTGGAC ATGGATCAAT 
TACAGCCGCT TCCAAGAGCT CGTTCTGCAG TACGAAGAGA GTGGAGGCTC CAAGACGTTC 
AGCTCGAGGG ATTACATGGC CAGAACCCCA CAGTGGGCAC TGTTTGGTGC CAGGGAAAGA 
GGCTTTGATC CCAAGGACAC GAGATACCAG AGGAAGAACA AAACGAAGGA CATTTCCGGA 
TGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tyw1 ( XM_006504331.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006504394.1 Mus musculus tRNA-yW synthesizing protein 1 homolog (S. cerevisiae) (Tyw1), transcript variant X1, mRNA.

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CloneID	OMu12980
Clone ID Related Accession (Same CDS sequence)	XM_006504331.1 , NM_001015876.2 , XM_006504331.2 , XM_006504331.3
Accession Version	XM_006504331.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2166bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006504331.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTCCTC TGGATGTGTG GGACTTGTCG CCTTTGCTGT CACTGTGGAT GAACAGGTTC 
TACATATATA TGGGCTGTGC CCTGGGCCTT ACCCTTTGCA TCTGTGTCCA GATCATCAAG 
AAGCAGGTCA CTCGTTCCCA GGAGAAACGT GTCCCAGGAG CTCCTGATAG CTCCCTCTCC 
CCTCAGAAGA AGCAGACACA CGTTTCTGGA GTGAAGATTT TTTACGGATC TCAGACGGGC 
ACTGCCAAGG GATTTGCAGT GGTTCTTGCC AAAGCTGTCA CCTCTCTGGA CCTGCCTGTG 
GCCATTATTA ATCTGAAGGA ATATGATCCA GATGACAGTC TGATAGGAGA GATCACTAGT 
AAAACCGTCT GTGCCTTCCT GGTCGCTACG TACACGGACG GCTGCCCTAC AGAGAGTGCA 
GAGTGGTTCT GCAAGTGGTT AGAGGAATCA GCTAATGATT TCCGCTTTGG CAAAACTTAC 
CTGAAGGGAC TTCGCTATGC AGTGTTTGGC CTGGGAGACT CTGCCTACCG GAGCCACTTC 
AACAAGGTCA GCACGAACGT TGACAAATGG CTCTGGATGC TCGGTGCCCA GCGGGTGCTG 
ACCCGAGGGG AAGGCGACTG CAATGCAGTT CAGAGCAAAC ATGGCAGCAT CGAGGCCGAC 
TTCACAGCCT GGAAGACCAA GTTCATCTCC CGGCTGCAGG CCCTGCAGAG AGGGGAGAAA 
AAGGCTTGTG GCGGGAACTG CAAGAGAGGG AAGTGTGAGT CGGCCCAGCA TGGCCCAGGA 
GAGGCGCGGC CTCACCCGCA AGGCGAATTG CATCCTGGGG ATGCTGAGGA GGAAGAGCCC 
TGTGAGAGCA GCAGCGAGGA CGAGCTCGGG ACCCAGGATT ATCAGAGCCT GACTTCTGTG 
GTGGATGTTG AGGACCTGGG CAACATCATG AACCCCGTGA AGAGGGAAAA GAGAGAAAAG 
TCTCATCAGG ACGGGAAAGC TGCCATGCAG AGGAACCCAG AGAAGACTGA GGACGGCGAA 
GGAAGAGCTA TGATAACACC TGCTCTGCGG GAAGCCCTGA CTAAACAAGG GTACCAGCTG 
ATTGGCAGCC ACTCTGGTGT GAAGCTTTGC AGGTGGACAA AGTCGATGCT GCGGGGGAGA 
GGAGGTTGCT ACAAACACAC ATTCTACGGC ATTGAGAGCC ACCGCTGCAT GGAAGCCACC 
CCAAGCTTGG CGTGTGCGAA TAAATGTGTC TTCTGCTGGC GGCACCATAC CAATCCTGTG 
GGCACTGAGT GGCGGTGGAA GATGGACCAG CCAGAATTGA TCTTGAAGGA AGCCATTGAG 
AACCATCAGA ACATGATTAA ACAGTTCAAA GGCGTGCCAG GCCTCAAGGC GGAGCGCTTT 
GAAGAGGGGA TGGAGGTCAA GCACTGTGCG CTGTCACTCG TGGGTGAGCC GATCATGTAC 
CCAGAAATCA ACAGGCTTTT GAAGCTGCTC CACCAGCACG GCATCTCCAG CTTCCTTGTC 
ACCAATGCGC AGTTCCCTGA GGAGATCAGG AAGCTCACAC CAGTTACTCA GCTCTACGTG 
AGCGTGGACG CCAGCACCAG AGACGGCCTG AAGAAGATCG ACCGCCCGCT CTTCAAGGAT 
TTCTGGCAGC GCTTCCTAGA CAGTTTGAAA GCCTTATCTG CAAAGCAACA ACGAACGGTC 
TACAGACTGA CGCTTGTGAA GTGTTGGAAC GTGGATGAAC TCCAGGCCTA CGCTGAGCTG 
GTGTCCTTGG GGAACCCCGA CTTCATCGAG GTCAAGGGTG TGACCTACTG TGGTGAGAGT 
GCAGCCAGCA GCCTCACCAT GGCCAATGTC CCTTGGCACG AGGAAGTGGT TCGGTTTGTC 
CGTGAGCTGG TCGATCTGCT TCCCGACTAT GAAGTTGCGT GTGAACACGA GCACTCCAAC 
TGCCTCCTGA TCGGACACAA GAAGTTTAAG ATCGATGGAG AATGGTGGAC ATGGATCAAT 
TACAGCCGCT TCCAAGAGCT CGTTCTGCAG TACGAAGAGA GTGGAGGCTC CAAGACGTTC 
AGCTCGAGGG ATTACATGGC CAGAACCCCA CAGTGGGCAC TGTTTGGTGC CAGGGAAAGA 
GGCTTTGATC CCAAGGACAC GAGATACCAG AGGAAGAACA AAACGAAGGA CATTTCCGGA 
TGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006504394.1
CDS	71..2236
Translation	MGPLDVWDLSPLLSLWMNRFYIYMGCALGLTLCICVQIIKKQVTRSQEKRVPGAPDSSLSPQKKQTHVSGVKIFYGSQTGTAKGFAVVLAKAVTSLDLPVAIINLKEYDPDDSLIGEITSKTVCAFLVATYTDGCPTESAEWFCKWLEESANDFRFGKTYLKGLRYAVFGLGDSAYRSHFNKVSTNVDKWLWMLGAQRVLTRGEGDCNAVQSKHGSIEADFTAWKTKFISRLQALQRGEKKACGGNCKRGKCESAQHGPGEARPHPQGELHPGDAEEEEPCESSSEDELGTQDYQSLTSVVDVEDLGNIMNPVKREKREKSHQDGKAAMQRNPEKTEDGEGRAMITPALREALTKQGYQLIGSHSGVKLCRWTKSMLRGRGGCYKHTFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGTEWRWKMDQPELILKEAIENHQNMIKQFKGVPGLKAERFEEGMEVKHCALSLVGEPIMYPEINRLLKLLHQHGISSFLVTNAQFPEEIRKLTPVTQLYVSVDASTRDGLKKIDRPLFKDFWQRFLDSLKALSAKQQRTVYRLTLVKCWNVDELQAYAELVSLGNPDFIEVKGVTYCGESAASSLTMANVPWHEEVVRFVRELVDLLPDYEVACEHEHSNCLLIGHKKFKIDGEWWTWINYSRFQELVLQYEESGGSKTFSSRDYMARTPQWALFGARERGFDPKDTRYQRKNKTKDISGC

Target ORF information:

RefSeq Version	XM_006504331.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus tRNA-yW synthesizing protein 1 homolog (S. cerevisiae) (Tyw1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006504331.3

ORF Insert Sequence:

ATGGGTCCTC TGGATGTGTG GGACTTGTCG CCTTTGCTGT CACTGTGGAT GAACAGGTTC 
TACATATATA TGGGCTGTGC CCTGGGCCTT ACCCTTTGCA TCTGTGTCCA GATCATCAAG 
AAGCAGGTCA CTCGTTCCCA GGAGAAACGT GTCCCAGGAG CTCCTGATAG CTCCCTCTCC 
CCTCAGAAGA AGCAGACACA CGTTTCTGGA GTGAAGATTT TTTACGGATC TCAGACGGGC 
ACTGCCAAGG GATTTGCAGT GGTTCTTGCC AAAGCTGTCA CCTCTCTGGA CCTGCCTGTG 
GCCATTATTA ATCTGAAGGA ATATGATCCA GATGACAGTC TGATAGGAGA GATCACTAGT 
AAAACCGTCT GTGCCTTCCT GGTCGCTACG TACACGGACG GCTGCCCTAC AGAGAGTGCA 
GAGTGGTTCT GCAAGTGGTT AGAGGAATCA GCTAATGATT TCCGCTTTGG CAAAACTTAC 
CTGAAGGGAC TTCGCTATGC AGTGTTTGGC CTGGGAGACT CTGCCTACCG GAGCCACTTC 
AACAAGGTCA GCACGAACGT TGACAAATGG CTCTGGATGC TCGGTGCCCA GCGGGTGCTG 
ACCCGAGGGG AAGGCGACTG CAATGCAGTT CAGAGCAAAC ATGGCAGCAT CGAGGCCGAC 
TTCACAGCCT GGAAGACCAA GTTCATCTCC CGGCTGCAGG CCCTGCAGAG AGGGGAGAAA 
AAGGCTTGTG GCGGGAACTG CAAGAGAGGG AAGTGTGAGT CGGCCCAGCA TGGCCCAGGA 
GAGGCGCGGC CTCACCCGCA AGGCGAATTG CATCCTGGGG ATGCTGAGGA GGAAGAGCCC 
TGTGAGAGCA GCAGCGAGGA CGAGCTCGGG ACCCAGGATT ATCAGAGCCT GACTTCTGTG 
GTGGATGTTG AGGACCTGGG CAACATCATG AACCCCGTGA AGAGGGAAAA GAGAGAAAAG 
TCTCATCAGG ACGGGAAAGC TGCCATGCAG AGGAACCCAG AGAAGACTGA GGACGGCGAA 
GGAAGAGCTA TGATAACACC TGCTCTGCGG GAAGCCCTGA CTAAACAAGG GTACCAGCTG 
ATTGGCAGCC ACTCTGGTGT GAAGCTTTGC AGGTGGACAA AGTCGATGCT GCGGGGGAGA 
GGAGGTTGCT ACAAACACAC ATTCTACGGC ATTGAGAGCC ACCGCTGCAT GGAAGCCACC 
CCAAGCTTGG CGTGTGCGAA TAAATGTGTC TTCTGCTGGC GGCACCATAC CAATCCTGTG 
GGCACTGAGT GGCGGTGGAA GATGGACCAG CCAGAATTGA TCTTGAAGGA AGCCATTGAG 
AACCATCAGA ACATGATTAA ACAGTTCAAA GGCGTGCCAG GCCTCAAGGC GGAGCGCTTT 
GAAGAGGGGA TGGAGGTCAA GCACTGTGCG CTGTCACTCG TGGGTGAGCC GATCATGTAC 
CCAGAAATCA ACAGGCTTTT GAAGCTGCTC CACCAGCACG GCATCTCCAG CTTCCTTGTC 
ACCAATGCGC AGTTCCCTGA GGAGATCAGG AAGCTCACAC CAGTTACTCA GCTCTACGTG 
AGCGTGGACG CCAGCACCAG AGACGGCCTG AAGAAGATCG ACCGCCCGCT CTTCAAGGAT 
TTCTGGCAGC GCTTCCTAGA CAGTTTGAAA GCCTTATCTG CAAAGCAACA ACGAACGGTC 
TACAGACTGA CGCTTGTGAA GTGTTGGAAC GTGGATGAAC TCCAGGCCTA CGCTGAGCTG 
GTGTCCTTGG GGAACCCCGA CTTCATCGAG GTCAAGGGTG TGACCTACTG TGGTGAGAGT 
GCAGCCAGCA GCCTCACCAT GGCCAATGTC CCTTGGCACG AGGAAGTGGT TCGGTTTGTC 
CGTGAGCTGG TCGATCTGCT TCCCGACTAT GAAGTTGCGT GTGAACACGA GCACTCCAAC 
TGCCTCCTGA TCGGACACAA GAAGTTTAAG ATCGATGGAG AATGGTGGAC ATGGATCAAT 
TACAGCCGCT TCCAAGAGCT CGTTCTGCAG TACGAAGAGA GTGGAGGCTC CAAGACGTTC 
AGCTCGAGGG ATTACATGGC CAGAACCCCA CAGTGGGCAC TGTTTGGTGC CAGGGAAAGA 
GGCTTTGATC CCAAGGACAC GAGATACCAG AGGAAGAACA AAACGAAGGA CATTTCCGGA 
TGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tyw1 ( XM_017320584.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017176073.1 Mus musculus tRNA-yW synthesizing protein 1 homolog (S. cerevisiae) (Tyw1), transcript variant X2, mRNA.

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CloneID	OMu81205
Clone ID Related Accession (Same CDS sequence)	XM_017320584.1 , XM_017320584.2
Accession Version	XM_017320584.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1590bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017320584.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCGGTG CCCAGCGGGT GCTGACCCGA GGGGAAGGCG ACTGCAATGC AGTTCAGAGC 
AAACATGGCA GCATCGAGGC CGACTTCACA GCCTGGAAGA CCAAGTTCAT CTCCCGGCTG 
CAGGCCCTGC AGAGAGGGGA GAAAAAGGCT TGTGGCGGGA ACTGCAAGAG AGGGAAGTGT 
GAGTCGGCCC AGCATGGCCC AGGAGAGGCG CGGCCTCACC CGCAAGGCGA ATTGCATCCT 
GGGGATGCTG AGGAGGAAGA GCCCTGTGAG AGCAGCAGCG AGGACGAGCT CGGGACCCAG 
GATTATCAGA GCCTGACTTC TGTGGTGGAT GTTGAGGACC TGGGCAACAT CATGAACCCC 
GTGAAGAGGG AAAAGAGAGA AAAGTCTCAT CAGGACGGGA AAGCTGCCAT GCAGAGGAAC 
CCAGAGAAGA CTGAGGACGG CGAAGGAAGA GCTATGATAA CACCTGCTCT GCGGGAAGCC 
CTGACTAAAC AAGGGTACCA GCTGATTGGC AGCCACTCTG GTGTGAAGCT TTGCAGGTGG 
ACAAAGTCGA TGCTGCGGGG GAGAGGAGGT TGCTACAAAC ACACATTCTA CGGCATTGAG 
AGCCACCGCT GCATGGAAGC CACCCCAAGC TTGGCGTGTG CGAATAAATG TGTCTTCTGC 
TGGCGGCACC ATACCAATCC TGTGGGCACT GAGTGGCGGT GGAAGATGGA CCAGCCAGAA 
TTGATCTTGA AGGAAGCCAT TGAGAACCAT CAGAACATGA TTAAACAGTT CAAAGGCGTG 
CCAGGCCTCA AGGCGGAGCG CTTTGAAGAG GGGATGGAGG TCAAGCACTG TGCGCTGTCA 
CTCGTGGGTG AGCCGATCAT GTACCCAGAA ATCAACAGGC TTTTGAAGCT GCTCCACCAG 
CACGGCATCT CCAGCTTCCT TGTCACCAAT GCGCAGTTCC CTGAGGAGAT CAGGAAGCTC 
ACACCAGTTA CTCAGCTCTA CGTGAGCGTG GACGCCAGCA CCAGAGACGG CCTGAAGAAG 
ATCGACCGCC CGCTCTTCAA GGATTTCTGG CAGCGCTTCC TAGACAGTTT GAAAGCCTTA 
TCTGCAAAGC AACAACGAAC GGTCTACAGA CTGACGCTTG TGAAGTGTTG GAACGTGGAT 
GAACTCCAGG CCTACGCTGA GCTGGTGTCC TTGGGGAACC CCGACTTCAT CGAGGTCAAG 
GGTGTGACCT ACTGTGGTGA GAGTGCAGCC AGCAGCCTCA CCATGGCCAA TGTCCCTTGG 
CACGAGGAAG TGGTTCGGTT TGTCCGTGAG CTGGTCGATC TGCTTCCCGA CTATGAAGTT 
GCGTGTGAAC ACGAGCACTC CAACTGCCTC CTGATCGGAC ACAAGAAGTT TAAGATCGAT 
GGAGAATGGT GGACATGGAT CAATTACAGC CGCTTCCAAG AGCTCGTTCT GCAGTACGAA 
GAGAGTGGAG GCTCCAAGAC GTTCAGCTCG AGGGATTACA TGGCCAGAAC CCCACAGTGG 
GCACTGTTTG GTGCCAGGGA AAGAGGCTTT GATCCCAAGG ACACGAGATA CCAGAGGAAG 
AACAAAACGA AGGACATTTC CGGATGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017176073.1
CDS	624..2213
Translation	MLGAQRVLTRGEGDCNAVQSKHGSIEADFTAWKTKFISRLQALQRGEKKACGGNCKRGKCESAQHGPGEARPHPQGELHPGDAEEEEPCESSSEDELGTQDYQSLTSVVDVEDLGNIMNPVKREKREKSHQDGKAAMQRNPEKTEDGEGRAMITPALREALTKQGYQLIGSHSGVKLCRWTKSMLRGRGGCYKHTFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGTEWRWKMDQPELILKEAIENHQNMIKQFKGVPGLKAERFEEGMEVKHCALSLVGEPIMYPEINRLLKLLHQHGISSFLVTNAQFPEEIRKLTPVTQLYVSVDASTRDGLKKIDRPLFKDFWQRFLDSLKALSAKQQRTVYRLTLVKCWNVDELQAYAELVSLGNPDFIEVKGVTYCGESAASSLTMANVPWHEEVVRFVRELVDLLPDYEVACEHEHSNCLLIGHKKFKIDGEWWTWINYSRFQELVLQYEESGGSKTFSSRDYMARTPQWALFGARERGFDPKDTRYQRKNKTKDISGC

Target ORF information:

RefSeq Version	XM_017320584.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus tRNA-yW synthesizing protein 1 homolog (S. cerevisiae) (Tyw1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017320584.2

ORF Insert Sequence:

ATGCTCGGTG CCCAGCGGGT GCTGACCCGA GGGGAAGGCG ACTGCAATGC AGTTCAGAGC 
AAACATGGCA GCATCGAGGC CGACTTCACA GCCTGGAAGA CCAAGTTCAT CTCCCGGCTG 
CAGGCCCTGC AGAGAGGGGA GAAAAAGGCT TGTGGCGGGA ACTGCAAGAG AGGGAAGTGT 
GAGTCGGCCC AGCATGGCCC AGGAGAGGCG CGGCCTCACC CGCAAGGCGA ATTGCATCCT 
GGGGATGCTG AGGAGGAAGA GCCCTGTGAG AGCAGCAGCG AGGACGAGCT CGGGACCCAG 
GATTATCAGA GCCTGACTTC TGTGGTGGAT GTTGAGGACC TGGGCAACAT CATGAACCCC 
GTGAAGAGGG AAAAGAGAGA AAAGTCTCAT CAGGACGGGA AAGCTGCCAT GCAGAGGAAC 
CCAGAGAAGA CTGAGGACGG CGAAGGAAGA GCTATGATAA CACCTGCTCT GCGGGAAGCC 
CTGACTAAAC AAGGGTACCA GCTGATTGGC AGCCACTCTG GTGTGAAGCT TTGCAGGTGG 
ACAAAGTCGA TGCTGCGGGG GAGAGGAGGT TGCTACAAAC ACACATTCTA CGGCATTGAG 
AGCCACCGCT GCATGGAAGC CACCCCAAGC TTGGCGTGTG CGAATAAATG TGTCTTCTGC 
TGGCGGCACC ATACCAATCC TGTGGGCACT GAGTGGCGGT GGAAGATGGA CCAGCCAGAA 
TTGATCTTGA AGGAAGCCAT TGAGAACCAT CAGAACATGA TTAAACAGTT CAAAGGCGTG 
CCAGGCCTCA AGGCGGAGCG CTTTGAAGAG GGGATGGAGG TCAAGCACTG TGCGCTGTCA 
CTCGTGGGTG AGCCGATCAT GTACCCAGAA ATCAACAGGC TTTTGAAGCT GCTCCACCAG 
CACGGCATCT CCAGCTTCCT TGTCACCAAT GCGCAGTTCC CTGAGGAGAT CAGGAAGCTC 
ACACCAGTTA CTCAGCTCTA CGTGAGCGTG GACGCCAGCA CCAGAGACGG CCTGAAGAAG 
ATCGACCGCC CGCTCTTCAA GGATTTCTGG CAGCGCTTCC TAGACAGTTT GAAAGCCTTA 
TCTGCAAAGC AACAACGAAC GGTCTACAGA CTGACGCTTG TGAAGTGTTG GAACGTGGAT 
GAACTCCAGG CCTACGCTGA GCTGGTGTCC TTGGGGAACC CCGACTTCAT CGAGGTCAAG 
GGTGTGACCT ACTGTGGTGA GAGTGCAGCC AGCAGCCTCA CCATGGCCAA TGTCCCTTGG 
CACGAGGAAG TGGTTCGGTT TGTCCGTGAG CTGGTCGATC TGCTTCCCGA CTATGAAGTT 
GCGTGTGAAC ACGAGCACTC CAACTGCCTC CTGATCGGAC ACAAGAAGTT TAAGATCGAT 
GGAGAATGGT GGACATGGAT CAATTACAGC CGCTTCCAAG AGCTCGTTCT GCAGTACGAA 
GAGAGTGGAG GCTCCAAGAC GTTCAGCTCG AGGGATTACA TGGCCAGAAC CCCACAGTGG 
GCACTGTTTG GTGCCAGGGA AAGAGGCTTT GATCCCAAGG ACACGAGATA CCAGAGGAAG 
AACAAAACGA AGGACATTTC CGGATGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

BayGenomics: a resource of insertional mutations in mouse embryonic stem cells.
Nucleic acids research31(1)278-81(2003 Jan)
Stryke D,Kawamoto M,Huang CC,Johns SJ,King LA,Harper CA,Meng EC,Lee RE,Yee A,L'Italien L,Chuang PT,Young SG,Skarnes WC,Babbitt PC,Ferrin TE

Construction of long-transcript enriched cDNA libraries from submicrogram amounts of total RNAs by a universal PCR amplification method.
Genome research11(9)1553-8(2001 Sep)
Piao Y,Ko NT,Lim MK,Ko MS

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Tyw1 cDNA ORF clone, Mus musculus(house mouse)

Gene

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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