The following Tyw1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Tyw1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID | OMu12990 | |
| Clone ID Related Accession (Same CDS sequence) | NM_178897.4 | |
| Accession Version | NM_178897.4 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 1365bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector |  User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-08-10 | |
| Organism | Mus musculus(house mouse) | |
| Product | S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase TYW1 isoform 2 | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK045279.1 and AC113029.23. On Aug 1, 2009 this sequence version replaced NM_178897.3. Transcript Variant: This variant (2) has multiple differences in its 3' end, compared to variant 1, which results in a protein (isoform 2) with a shorter and distinct C-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AK045279.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## | |
1 | ATGGGTCCTC TGGATGTGTG GGACTTGTCG CCTTTGCTGT CACTGTGGAT GAACAGGTTC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_849228.1 |
| CDS | 24..1388 |
| Translation |
Target ORF information:
Target ORF information:
|
 NM_178897.4 |
1 | ATGGGTCCTC TGGATGTGTG GGACTTGTCG CCTTTGCTGT CACTGTGGAT GAACAGGTTC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OMu81205 | |
| Clone ID Related Accession (Same CDS sequence) | XM_017320584.1 , XM_017320584.2 | |
| Accession Version | XM_017320584.1 | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 1590bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2016-06-21 | |
| Organism | Mus musculus(house mouse) | |
| Product | S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase isoform X2 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGCTCGGTG CCCAGCGGGT GCTGACCCGA GGGGAAGGCG ACTGCAATGC AGTTCAGAGC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_017176073.1 |
| CDS | 624..2213 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_017320584.1 |
1 | ATGCTCGGTG CCCAGCGGGT GCTGACCCGA GGGGAAGGCG ACTGCAATGC AGTTCAGAGC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OMu12980 | |
| Clone ID Related Accession (Same CDS sequence) | XM_006504331.1 , NM_001015876.2 , XM_006504331.2 , XM_006504331.3 | |
| Accession Version | NM_001015876.2 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 2166bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-08-10 | |
| Organism | Mus musculus(house mouse) | |
| Product | S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase TYW1 isoform 1 | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BB619468.1, BC068126.1, CD551326.1 and BY670442.1. On Aug 1, 2009 this sequence version replaced NM_001015876.1. Transcript Variant: This variant (1) encodes the longer isoform (1). ##Evidence-Data-START## Transcript exon combination :: BC068126.1, AK082884.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. | |
1 | ATGGGTCCTC TGGATGTGTG GGACTTGTCG CCTTTGCTGT CACTGTGGAT GAACAGGTTC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_001015876.1 |
| CDS | 154..2319 |
| Translation |
Target ORF information:
Target ORF information:
|
NM_001015876.2 |
1 | ATGGGTCCTC TGGATGTGTG GGACTTGTCG CCTTTGCTGT CACTGTGGAT GAACAGGTTC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OMu12980 | |
| Clone ID Related Accession (Same CDS sequence) | XM_006504331.1 , NM_001015876.2 , XM_006504331.2 , XM_006504331.3 | |
| Accession Version | XM_006504331.1 | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 2166bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2015-02-08 | |
| Organism | Mus musculus(House mouse) | |
| Product | S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase isoform X1 | |
| Comment | ||
1 | ATGGGTCCTC TGGATGTGTG GGACTTGTCG CCTTTGCTGT CACTGTGGAT GAACAGGTTC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_006504394.1 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_006504331.1 |
1 | ATGGGTCCTC TGGATGTGTG GGACTTGTCG CCTTTGCTGT CACTGTGGAT GAACAGGTTC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OMu12980 | |
| Clone ID Related Accession (Same CDS sequence) | XM_006504331.1 , NM_001015876.2 , XM_006504331.2 , XM_006504331.3 | |
| Accession Version | XM_006504331.2 | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 2166bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2016-06-21 | |
| Organism | Mus musculus(house mouse) | |
| Product | S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006504331.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGGGTCCTC TGGATGTGTG GGACTTGTCG CCTTTGCTGT CACTGTGGAT GAACAGGTTC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_006504394.1 |
| CDS | 71..2236 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_006504331.2 |
1 | ATGGGTCCTC TGGATGTGTG GGACTTGTCG CCTTTGCTGT CACTGTGGAT GAACAGGTTC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OMu12980 | |
| Clone ID Related Accession (Same CDS sequence) | XM_006504331.1 , NM_001015876.2 , XM_006504331.2 , XM_006504331.3 | |
| Accession Version | XM_006504331.3 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 2166bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-08-07 | |
| Organism | Mus musculus(house mouse) | |
| Product | S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006504331.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGGGTCCTC TGGATGTGTG GGACTTGTCG CCTTTGCTGT CACTGTGGAT GAACAGGTTC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_006504394.1 |
| CDS | 71..2236 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_006504331.3 |
1 | ATGGGTCCTC TGGATGTGTG GGACTTGTCG CCTTTGCTGT CACTGTGGAT GAACAGGTTC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OMu81205 | |
| Clone ID Related Accession (Same CDS sequence) | XM_017320584.1 , XM_017320584.2 | |
| Accession Version | XM_017320584.2 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 1590bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-08-07 | |
| Organism | Mus musculus(house mouse) | |
| Product | S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase isoform X2 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017320584.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGCTCGGTG CCCAGCGGGT GCTGACCCGA GGGGAAGGCG ACTGCAATGC AGTTCAGAGC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_017176073.1 |
| CDS | 624..2213 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_017320584.2 |
1 | ATGCTCGGTG CCCAGCGGGT GCTGACCCGA GGGGAAGGCG ACTGCAATGC AGTTCAGAGC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
BayGenomics: a resource of insertional mutations in mouse embryonic stem cells. |
Construction of long-transcript enriched cDNA libraries from submicrogram amounts of total RNAs by a universal PCR amplification method. |
