HMGCS1 cDNA ORF clone, Canis lupus familiaris(dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	HMGCS1
Entrez Gene ID	479344
Full Name	3-hydroxy-3-methylglutaryl-CoA synthase 1
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005619344.1	XP_005619401.1	hydroxymethylglutaryl-CoA synthase, cytoplasmic
XM_005619343.2	XP_005619400.1	hydroxymethylglutaryl-CoA synthase, cytoplasmic
XM_022417862.1	XP_022273570.1	hydroxymethylglutaryl-CoA synthase, cytoplasmic isoform X1
XM_536483.4	XP_536483.3	hydroxymethylglutaryl-CoA synthase, cytoplasmic
XM_005619344.2	XP_005619401.1	hydroxymethylglutaryl-CoA synthase, cytoplasmic isoform X2
XM_022417863.1	XP_022273571.1	hydroxymethylglutaryl-CoA synthase, cytoplasmic isoform X1
XM_536483.5	XP_536483.3	hydroxymethylglutaryl-CoA synthase, cytoplasmic isoform X2

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Hmgcs1	NP_666054.2
Kluyveromyces lactis	KLLA0D10505g	XP_453529.2
Eremothecium gossypii	AGOS_ADL356C	NP_983739.1
Magnaporthe oryzae (rice blast fungus)	MGG_01026	XP_003717917.1
Neurospora crassa	NCU03922	XP_957561.1
Oryza sativa (rice)	Os08g0544900	NP_001062410.1
Canis lupus familiaris (dog)	HMGCS1	XP_005619400.1
Bos taurus (cattle)	HMGCS1	NP_001193507.1
Danio rerio (zebrafish)	hmgcs1	NP_957379.1
Arabidopsis thaliana (thale cress)	MVA1	NP_192919.1
Oryza sativa (rice)	Os03g0118800	NP_001048774.1
Oryza sativa (rice)	Os09g0521400	NP_001063706.1
	HMGCS1	XP_002804403.1
Saccharomyces cerevisiae (baker's yeast)	ERG13	NP_013580.1
Xenopus tropicalis (tropical clawed frog)	hmgcs1	NP_001120172.1
Homo sapiens (human)	HMGCS1	NP_001091742.1
Rattus norvegicus (Norway rat)	Hmgcs1	NP_058964.1
Pan troglodytes (chimpanzee)	HMGCS1	XP_003310783.1
Gallus gallus (chicken)	HMGCS1	NP_990742.1
Drosophila melanogaster (fruit fly)	Hmgs	NP_725570.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005847	XP_315872.3
Schizosaccharomyces pombe (fission yeast)	hcs1	NP_593859.1

Gene Ontology

The following HMGCS1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HMGCS1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe05473	XM_536483.5 Latest version!	Canis lupus familiaris 3-hydroxy-3-methylglutaryl-CoA synthase 1 (HMGCS1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OCe05473	XM_005619343.2 Latest version!	Canis lupus familiaris 3-hydroxy-3-methylglutaryl-CoA synthase 1 (soluble) (HMGCS1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OCe05473	XM_005619344.2 Latest version!	Canis lupus familiaris 3-hydroxy-3-methylglutaryl-CoA synthase 1 (HMGCS1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OCe287715	XM_022417863.1 Latest version!	Canis lupus familiaris 3-hydroxy-3-methylglutaryl-CoA synthase 1 (HMGCS1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
OCe287715	XM_022417862.1 Latest version!	Canis lupus familiaris 3-hydroxy-3-methylglutaryl-CoA synthase 1 (HMGCS1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
View Old Accession Versions >>
OCe05473	XM_005619344.1	Canis lupus familiaris 3-hydroxy-3-methylglutaryl-CoA synthase 1 (soluble) (HMGCS1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OCe05473	XM_536483.4	Canis lupus familiaris 3-hydroxy-3-methylglutaryl-CoA synthase 1 (soluble) (HMGCS1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HMGCS1 ( XM_005619344.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005619401.1 Canis lupus familiaris 3-hydroxy-3-methylglutaryl-CoA synthase 1 (soluble) (HMGCS1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OCe05473
Clone ID Related Accession (Same CDS sequence)	XM_005619344.1 , XM_005619343.2 , XM_536483.4 , XM_005619344.2 , XM_536483.5
Accession Version	XM_005619344.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1563bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	hydroxymethylglutaryl-CoA synthase, cytoplasmic
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726056.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGGGT CACTTCCTTT GAATGCAGAA GCCTGCTGGC CAAAAGATGT GGGAATTGTT 
GCCCTTGAGA TCTATTTTCC TTCTCAATAT GTTGATCAAG CAGAGTTGGA AAAATATGAT 
GGTGTAGATG CTGGAAAGTA TACTATTGGC TTGGGCCAGG CCAAGATGGG CTTCTGCACG 
GATAGAGAAG ATATCAACTC TCTTTGCCTG ACTGTGGTTC AGAACCTTAT GGAGAGACAT 
AGCCTTTCGT ATGATTGCAT TGGGCGTTTA GAAGTTGGAA CAGAGACAAT CATTGACAAA 
TCAAAATCCG TGAAGACTAA TTTGATGCAG CTCTTTGAGG AGTCTGGGAA CACAGATATA 
GAAGGAATAG ATACAACTAA TGCATGCTAT GGAGGCACAG CCGCTGTCTT CAATGCTGTT 
AATTGGATTG AGTCCAGCTC CTGGGATGGA CGGTATGCCC TGGTAGTTGC GGGAGATATT 
GCTGTATATG CCACAGGAAA TGCTAGACCT ACAGGTGGAG TGGGAGCTGT TGCTCTGCTA 
ATTGGGCCAA ATGCGCCTTT AATTTTTGAC CGAGGACTTC GTGGGACACA TATGCAGCAT 
GCCTATGACT TTTACAAGCC TGACATGCTT TCAGAATATC CTATAGTAGA TGGCAAACTC 
TCCATACAGT GCTACCTCAG TGCACTAGAC CGCTGCTACT CTGTCTACCG CAAAAAGATC 
CGAGCCCAGT GGCAGAAAGA GGGAAATGAT AAAGATTTTA CCTTGAATGA TTTTGGTTTC 
ATGATCTTCC ACTCGCCCTA CTGTAAGCTG GTTCAGAAGT CTCTAGCTCG GATGTTGCTG 
AATGACTTCC TTAATGACCA GAACAGAGAT AAAAATAGTA TATATAGTGG CCTGGAAGCC 
TTTGGGGATG TTAAATTAGA AGATACCTAC TTTGATAGAG ATGTGGAAAA GGCATTTATG 
AAGGCTAGTG CTGAACTCTT TAATCAGAAA ACAAAGGCAT CTTTGCTTGT GTCAAATCAA 
AATGGAAATA TGTACACATC GTCTGTTTAT GGCTCCCTCG CTTCTGTTCT AGCACAGTAC 
TCCCCTCAGC AGCTGGCAGG AAAGAGGATT GGTGTGTTCT CTTACGGTTC TGGTTTGGCT 
GCCACCCTGT ACTCCCTTAG AGTTACACAA GATGCCACAC CAGGGTCTGC TCTTGATAAA 
ATAACAGCAA GTTTATGTGA CCTCAAATCA AGACTTGACT CAAGAACTTG TGTGGCACCA 
GATGTCTTTG CTGAAAACAT GAAGCTCAGA GAGGACACTC ATCACTTGGT CAACTATATT 
CCTCAGAGTT CAGTAGATTC CCTCTTCGAA GGGACCTGGT ACCTCGTCAG AGTGGATGAG 
AAGCACAGGA GGACCTATGC CCGGCGCCCC TCCCCCAGTG AGGATACTCT GGGTGAAGGA 
GTAGGACTCG TACATTCAAG TGCAGCCACT GAGCATATCC CAAGTCCTGC TAAGAAAGTG 
CCAAGACTCC CTGCCACAGC AGCAGAAGCC GAAGCAGCTG TCATTAGTAA CGGGGAGCAT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005619401.1
CDS	145..1707
Translation	MPGSLPLNAEACWPKDVGIVALEIYFPSQYVDQAELEKYDGVDAGKYTIGLGQAKMGFCTDREDINSLCLTVVQNLMERHSLSYDCIGRLEVGTETIIDKSKSVKTNLMQLFEESGNTDIEGIDTTNACYGGTAAVFNAVNWIESSSWDGRYALVVAGDIAVYATGNARPTGGVGAVALLIGPNAPLIFDRGLRGTHMQHAYDFYKPDMLSEYPIVDGKLSIQCYLSALDRCYSVYRKKIRAQWQKEGNDKDFTLNDFGFMIFHSPYCKLVQKSLARMLLNDFLNDQNRDKNSIYSGLEAFGDVKLEDTYFDRDVEKAFMKASAELFNQKTKASLLVSNQNGNMYTSSVYGSLASVLAQYSPQQLAGKRIGVFSYGSGLAATLYSLRVTQDATPGSALDKITASLCDLKSRLDSRTCVAPDVFAENMKLREDTHHLVNYIPQSSVDSLFEGTWYLVRVDEKHRRTYARRPSPSEDTLGEGVGLVHSSAATEHIPSPAKKVPRLPATAAEAEAAVISNGEH

Target ORF information:

RefSeq Version	XM_005619344.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris 3-hydroxy-3-methylglutaryl-CoA synthase 1 (soluble) (HMGCS1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005619344.1

ORF Insert Sequence:

ATGCCTGGGT CACTTCCTTT GAATGCAGAA GCCTGCTGGC CAAAAGATGT GGGAATTGTT 
GCCCTTGAGA TCTATTTTCC TTCTCAATAT GTTGATCAAG CAGAGTTGGA AAAATATGAT 
GGTGTAGATG CTGGAAAGTA TACTATTGGC TTGGGCCAGG CCAAGATGGG CTTCTGCACG 
GATAGAGAAG ATATCAACTC TCTTTGCCTG ACTGTGGTTC AGAACCTTAT GGAGAGACAT 
AGCCTTTCGT ATGATTGCAT TGGGCGTTTA GAAGTTGGAA CAGAGACAAT CATTGACAAA 
TCAAAATCCG TGAAGACTAA TTTGATGCAG CTCTTTGAGG AGTCTGGGAA CACAGATATA 
GAAGGAATAG ATACAACTAA TGCATGCTAT GGAGGCACAG CCGCTGTCTT CAATGCTGTT 
AATTGGATTG AGTCCAGCTC CTGGGATGGA CGGTATGCCC TGGTAGTTGC GGGAGATATT 
GCTGTATATG CCACAGGAAA TGCTAGACCT ACAGGTGGAG TGGGAGCTGT TGCTCTGCTA 
ATTGGGCCAA ATGCGCCTTT AATTTTTGAC CGAGGACTTC GTGGGACACA TATGCAGCAT 
GCCTATGACT TTTACAAGCC TGACATGCTT TCAGAATATC CTATAGTAGA TGGCAAACTC 
TCCATACAGT GCTACCTCAG TGCACTAGAC CGCTGCTACT CTGTCTACCG CAAAAAGATC 
CGAGCCCAGT GGCAGAAAGA GGGAAATGAT AAAGATTTTA CCTTGAATGA TTTTGGTTTC 
ATGATCTTCC ACTCGCCCTA CTGTAAGCTG GTTCAGAAGT CTCTAGCTCG GATGTTGCTG 
AATGACTTCC TTAATGACCA GAACAGAGAT AAAAATAGTA TATATAGTGG CCTGGAAGCC 
TTTGGGGATG TTAAATTAGA AGATACCTAC TTTGATAGAG ATGTGGAAAA GGCATTTATG 
AAGGCTAGTG CTGAACTCTT TAATCAGAAA ACAAAGGCAT CTTTGCTTGT GTCAAATCAA 
AATGGAAATA TGTACACATC GTCTGTTTAT GGCTCCCTCG CTTCTGTTCT AGCACAGTAC 
TCCCCTCAGC AGCTGGCAGG AAAGAGGATT GGTGTGTTCT CTTACGGTTC TGGTTTGGCT 
GCCACCCTGT ACTCCCTTAG AGTTACACAA GATGCCACAC CAGGGTCTGC TCTTGATAAA 
ATAACAGCAA GTTTATGTGA CCTCAAATCA AGACTTGACT CAAGAACTTG TGTGGCACCA 
GATGTCTTTG CTGAAAACAT GAAGCTCAGA GAGGACACTC ATCACTTGGT CAACTATATT 
CCTCAGAGTT CAGTAGATTC CCTCTTCGAA GGGACCTGGT ACCTCGTCAG AGTGGATGAG 
AAGCACAGGA GGACCTATGC CCGGCGCCCC TCCCCCAGTG AGGATACTCT GGGTGAAGGA 
GTAGGACTCG TACATTCAAG TGCAGCCACT GAGCATATCC CAAGTCCTGC TAAGAAAGTG 
CCAAGACTCC CTGCCACAGC AGCAGAAGCC GAAGCAGCTG TCATTAGTAA CGGGGAGCAT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HMGCS1 ( XM_005619343.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005619400.1 Canis lupus familiaris 3-hydroxy-3-methylglutaryl-CoA synthase 1 (soluble) (HMGCS1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCe05473
Clone ID Related Accession (Same CDS sequence)	XM_005619344.1 , XM_005619343.2 , XM_536483.4 , XM_005619344.2 , XM_536483.5
Accession Version	XM_005619343.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1563bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	hydroxymethylglutaryl-CoA synthase, cytoplasmic
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726056.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005619343.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGGGT CACTTCCTTT GAATGCAGAA GCCTGCTGGC CAAAAGATGT GGGAATTGTT 
GCCCTTGAGA TCTATTTTCC TTCTCAATAT GTTGATCAAG CAGAGTTGGA AAAATATGAT 
GGTGTAGATG CTGGAAAGTA TACTATTGGC TTGGGCCAGG CCAAGATGGG CTTCTGCACG 
GATAGAGAAG ATATCAACTC TCTTTGCCTG ACTGTGGTTC AGAACCTTAT GGAGAGACAT 
AGCCTTTCGT ATGATTGCAT TGGGCGTTTA GAAGTTGGAA CAGAGACAAT CATTGACAAA 
TCAAAATCCG TGAAGACTAA TTTGATGCAG CTCTTTGAGG AGTCTGGGAA CACAGATATA 
GAAGGAATAG ATACAACTAA TGCATGCTAT GGAGGCACAG CCGCTGTCTT CAATGCTGTT 
AATTGGATTG AGTCCAGCTC CTGGGATGGA CGGTATGCCC TGGTAGTTGC GGGAGATATT 
GCTGTATATG CCACAGGAAA TGCTAGACCT ACAGGTGGAG TGGGAGCTGT TGCTCTGCTA 
ATTGGGCCAA ATGCGCCTTT AATTTTTGAC CGAGGACTTC GTGGGACACA TATGCAGCAT 
GCCTATGACT TTTACAAGCC TGACATGCTT TCAGAATATC CTATAGTAGA TGGCAAACTC 
TCCATACAGT GCTACCTCAG TGCACTAGAC CGCTGCTACT CTGTCTACCG CAAAAAGATC 
CGAGCCCAGT GGCAGAAAGA GGGAAATGAT AAAGATTTTA CCTTGAATGA TTTTGGTTTC 
ATGATCTTCC ACTCGCCCTA CTGTAAGCTG GTTCAGAAGT CTCTAGCTCG GATGTTGCTG 
AATGACTTCC TTAATGACCA GAACAGAGAT AAAAATAGTA TATATAGTGG CCTGGAAGCC 
TTTGGGGATG TTAAATTAGA AGATACCTAC TTTGATAGAG ATGTGGAAAA GGCATTTATG 
AAGGCTAGTG CTGAACTCTT TAATCAGAAA ACAAAGGCAT CTTTGCTTGT GTCAAATCAA 
AATGGAAATA TGTACACATC GTCTGTTTAT GGCTCCCTCG CTTCTGTTCT AGCACAGTAC 
TCCCCTCAGC AGCTGGCAGG AAAGAGGATT GGTGTGTTCT CTTACGGTTC TGGTTTGGCT 
GCCACCCTGT ACTCCCTTAG AGTTACACAA GATGCCACAC CAGGGTCTGC TCTTGATAAA 
ATAACAGCAA GTTTATGTGA CCTCAAATCA AGACTTGACT CAAGAACTTG TGTGGCACCA 
GATGTCTTTG CTGAAAACAT GAAGCTCAGA GAGGACACTC ATCACTTGGT CAACTATATT 
CCTCAGAGTT CAGTAGATTC CCTCTTCGAA GGGACCTGGT ACCTCGTCAG AGTGGATGAG 
AAGCACAGGA GGACCTATGC CCGGCGCCCC TCCCCCAGTG AGGATACTCT GGGTGAAGGA 
GTAGGACTCG TACATTCAAG TGCAGCCACT GAGCATATCC CAAGTCCTGC TAAGAAAGTG 
CCAAGACTCC CTGCCACAGC AGCAGAAGCC GAAGCAGCTG TCATTAGTAA CGGGGAGCAT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005619400.1
CDS	206..1768
Translation	MPGSLPLNAEACWPKDVGIVALEIYFPSQYVDQAELEKYDGVDAGKYTIGLGQAKMGFCTDREDINSLCLTVVQNLMERHSLSYDCIGRLEVGTETIIDKSKSVKTNLMQLFEESGNTDIEGIDTTNACYGGTAAVFNAVNWIESSSWDGRYALVVAGDIAVYATGNARPTGGVGAVALLIGPNAPLIFDRGLRGTHMQHAYDFYKPDMLSEYPIVDGKLSIQCYLSALDRCYSVYRKKIRAQWQKEGNDKDFTLNDFGFMIFHSPYCKLVQKSLARMLLNDFLNDQNRDKNSIYSGLEAFGDVKLEDTYFDRDVEKAFMKASAELFNQKTKASLLVSNQNGNMYTSSVYGSLASVLAQYSPQQLAGKRIGVFSYGSGLAATLYSLRVTQDATPGSALDKITASLCDLKSRLDSRTCVAPDVFAENMKLREDTHHLVNYIPQSSVDSLFEGTWYLVRVDEKHRRTYARRPSPSEDTLGEGVGLVHSSAATEHIPSPAKKVPRLPATAAEAEAAVISNGEH

Target ORF information:

RefSeq Version	XM_005619343.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris 3-hydroxy-3-methylglutaryl-CoA synthase 1 (soluble) (HMGCS1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005619343.2

ORF Insert Sequence:

ATGCCTGGGT CACTTCCTTT GAATGCAGAA GCCTGCTGGC CAAAAGATGT GGGAATTGTT 
GCCCTTGAGA TCTATTTTCC TTCTCAATAT GTTGATCAAG CAGAGTTGGA AAAATATGAT 
GGTGTAGATG CTGGAAAGTA TACTATTGGC TTGGGCCAGG CCAAGATGGG CTTCTGCACG 
GATAGAGAAG ATATCAACTC TCTTTGCCTG ACTGTGGTTC AGAACCTTAT GGAGAGACAT 
AGCCTTTCGT ATGATTGCAT TGGGCGTTTA GAAGTTGGAA CAGAGACAAT CATTGACAAA 
TCAAAATCCG TGAAGACTAA TTTGATGCAG CTCTTTGAGG AGTCTGGGAA CACAGATATA 
GAAGGAATAG ATACAACTAA TGCATGCTAT GGAGGCACAG CCGCTGTCTT CAATGCTGTT 
AATTGGATTG AGTCCAGCTC CTGGGATGGA CGGTATGCCC TGGTAGTTGC GGGAGATATT 
GCTGTATATG CCACAGGAAA TGCTAGACCT ACAGGTGGAG TGGGAGCTGT TGCTCTGCTA 
ATTGGGCCAA ATGCGCCTTT AATTTTTGAC CGAGGACTTC GTGGGACACA TATGCAGCAT 
GCCTATGACT TTTACAAGCC TGACATGCTT TCAGAATATC CTATAGTAGA TGGCAAACTC 
TCCATACAGT GCTACCTCAG TGCACTAGAC CGCTGCTACT CTGTCTACCG CAAAAAGATC 
CGAGCCCAGT GGCAGAAAGA GGGAAATGAT AAAGATTTTA CCTTGAATGA TTTTGGTTTC 
ATGATCTTCC ACTCGCCCTA CTGTAAGCTG GTTCAGAAGT CTCTAGCTCG GATGTTGCTG 
AATGACTTCC TTAATGACCA GAACAGAGAT AAAAATAGTA TATATAGTGG CCTGGAAGCC 
TTTGGGGATG TTAAATTAGA AGATACCTAC TTTGATAGAG ATGTGGAAAA GGCATTTATG 
AAGGCTAGTG CTGAACTCTT TAATCAGAAA ACAAAGGCAT CTTTGCTTGT GTCAAATCAA 
AATGGAAATA TGTACACATC GTCTGTTTAT GGCTCCCTCG CTTCTGTTCT AGCACAGTAC 
TCCCCTCAGC AGCTGGCAGG AAAGAGGATT GGTGTGTTCT CTTACGGTTC TGGTTTGGCT 
GCCACCCTGT ACTCCCTTAG AGTTACACAA GATGCCACAC CAGGGTCTGC TCTTGATAAA 
ATAACAGCAA GTTTATGTGA CCTCAAATCA AGACTTGACT CAAGAACTTG TGTGGCACCA 
GATGTCTTTG CTGAAAACAT GAAGCTCAGA GAGGACACTC ATCACTTGGT CAACTATATT 
CCTCAGAGTT CAGTAGATTC CCTCTTCGAA GGGACCTGGT ACCTCGTCAG AGTGGATGAG 
AAGCACAGGA GGACCTATGC CCGGCGCCCC TCCCCCAGTG AGGATACTCT GGGTGAAGGA 
GTAGGACTCG TACATTCAAG TGCAGCCACT GAGCATATCC CAAGTCCTGC TAAGAAAGTG 
CCAAGACTCC CTGCCACAGC AGCAGAAGCC GAAGCAGCTG TCATTAGTAA CGGGGAGCAT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HMGCS1 ( XM_536483.4 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_536483.3 Canis lupus familiaris 3-hydroxy-3-methylglutaryl-CoA synthase 1 (soluble) (HMGCS1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe05473
Clone ID Related Accession (Same CDS sequence)	XM_005619344.1 , XM_005619343.2 , XM_536483.4 , XM_005619344.2 , XM_536483.5
Accession Version	XM_536483.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1563bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	hydroxymethylglutaryl-CoA synthase, cytoplasmic
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726056.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 24, 2013 this sequence version replaced XM_536483.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGGGT CACTTCCTTT GAATGCAGAA GCCTGCTGGC CAAAAGATGT GGGAATTGTT 
GCCCTTGAGA TCTATTTTCC TTCTCAATAT GTTGATCAAG CAGAGTTGGA AAAATATGAT 
GGTGTAGATG CTGGAAAGTA TACTATTGGC TTGGGCCAGG CCAAGATGGG CTTCTGCACG 
GATAGAGAAG ATATCAACTC TCTTTGCCTG ACTGTGGTTC AGAACCTTAT GGAGAGACAT 
AGCCTTTCGT ATGATTGCAT TGGGCGTTTA GAAGTTGGAA CAGAGACAAT CATTGACAAA 
TCAAAATCCG TGAAGACTAA TTTGATGCAG CTCTTTGAGG AGTCTGGGAA CACAGATATA 
GAAGGAATAG ATACAACTAA TGCATGCTAT GGAGGCACAG CCGCTGTCTT CAATGCTGTT 
AATTGGATTG AGTCCAGCTC CTGGGATGGA CGGTATGCCC TGGTAGTTGC GGGAGATATT 
GCTGTATATG CCACAGGAAA TGCTAGACCT ACAGGTGGAG TGGGAGCTGT TGCTCTGCTA 
ATTGGGCCAA ATGCGCCTTT AATTTTTGAC CGAGGACTTC GTGGGACACA TATGCAGCAT 
GCCTATGACT TTTACAAGCC TGACATGCTT TCAGAATATC CTATAGTAGA TGGCAAACTC 
TCCATACAGT GCTACCTCAG TGCACTAGAC CGCTGCTACT CTGTCTACCG CAAAAAGATC 
CGAGCCCAGT GGCAGAAAGA GGGAAATGAT AAAGATTTTA CCTTGAATGA TTTTGGTTTC 
ATGATCTTCC ACTCGCCCTA CTGTAAGCTG GTTCAGAAGT CTCTAGCTCG GATGTTGCTG 
AATGACTTCC TTAATGACCA GAACAGAGAT AAAAATAGTA TATATAGTGG CCTGGAAGCC 
TTTGGGGATG TTAAATTAGA AGATACCTAC TTTGATAGAG ATGTGGAAAA GGCATTTATG 
AAGGCTAGTG CTGAACTCTT TAATCAGAAA ACAAAGGCAT CTTTGCTTGT GTCAAATCAA 
AATGGAAATA TGTACACATC GTCTGTTTAT GGCTCCCTCG CTTCTGTTCT AGCACAGTAC 
TCCCCTCAGC AGCTGGCAGG AAAGAGGATT GGTGTGTTCT CTTACGGTTC TGGTTTGGCT 
GCCACCCTGT ACTCCCTTAG AGTTACACAA GATGCCACAC CAGGGTCTGC TCTTGATAAA 
ATAACAGCAA GTTTATGTGA CCTCAAATCA AGACTTGACT CAAGAACTTG TGTGGCACCA 
GATGTCTTTG CTGAAAACAT GAAGCTCAGA GAGGACACTC ATCACTTGGT CAACTATATT 
CCTCAGAGTT CAGTAGATTC CCTCTTCGAA GGGACCTGGT ACCTCGTCAG AGTGGATGAG 
AAGCACAGGA GGACCTATGC CCGGCGCCCC TCCCCCAGTG AGGATACTCT GGGTGAAGGA 
GTAGGACTCG TACATTCAAG TGCAGCCACT GAGCATATCC CAAGTCCTGC TAAGAAAGTG 
CCAAGACTCC CTGCCACAGC AGCAGAAGCC GAAGCAGCTG TCATTAGTAA CGGGGAGCAT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_536483.3
CDS	206..1768
Translation	MPGSLPLNAEACWPKDVGIVALEIYFPSQYVDQAELEKYDGVDAGKYTIGLGQAKMGFCTDREDINSLCLTVVQNLMERHSLSYDCIGRLEVGTETIIDKSKSVKTNLMQLFEESGNTDIEGIDTTNACYGGTAAVFNAVNWIESSSWDGRYALVVAGDIAVYATGNARPTGGVGAVALLIGPNAPLIFDRGLRGTHMQHAYDFYKPDMLSEYPIVDGKLSIQCYLSALDRCYSVYRKKIRAQWQKEGNDKDFTLNDFGFMIFHSPYCKLVQKSLARMLLNDFLNDQNRDKNSIYSGLEAFGDVKLEDTYFDRDVEKAFMKASAELFNQKTKASLLVSNQNGNMYTSSVYGSLASVLAQYSPQQLAGKRIGVFSYGSGLAATLYSLRVTQDATPGSALDKITASLCDLKSRLDSRTCVAPDVFAENMKLREDTHHLVNYIPQSSVDSLFEGTWYLVRVDEKHRRTYARRPSPSEDTLGEGVGLVHSSAATEHIPSPAKKVPRLPATAAEAEAAVISNGEH

Target ORF information:

RefSeq Version	XM_536483.4
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris 3-hydroxy-3-methylglutaryl-CoA synthase 1 (soluble) (HMGCS1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_536483.4

ORF Insert Sequence:

ATGCCTGGGT CACTTCCTTT GAATGCAGAA GCCTGCTGGC CAAAAGATGT GGGAATTGTT 
GCCCTTGAGA TCTATTTTCC TTCTCAATAT GTTGATCAAG CAGAGTTGGA AAAATATGAT 
GGTGTAGATG CTGGAAAGTA TACTATTGGC TTGGGCCAGG CCAAGATGGG CTTCTGCACG 
GATAGAGAAG ATATCAACTC TCTTTGCCTG ACTGTGGTTC AGAACCTTAT GGAGAGACAT 
AGCCTTTCGT ATGATTGCAT TGGGCGTTTA GAAGTTGGAA CAGAGACAAT CATTGACAAA 
TCAAAATCCG TGAAGACTAA TTTGATGCAG CTCTTTGAGG AGTCTGGGAA CACAGATATA 
GAAGGAATAG ATACAACTAA TGCATGCTAT GGAGGCACAG CCGCTGTCTT CAATGCTGTT 
AATTGGATTG AGTCCAGCTC CTGGGATGGA CGGTATGCCC TGGTAGTTGC GGGAGATATT 
GCTGTATATG CCACAGGAAA TGCTAGACCT ACAGGTGGAG TGGGAGCTGT TGCTCTGCTA 
ATTGGGCCAA ATGCGCCTTT AATTTTTGAC CGAGGACTTC GTGGGACACA TATGCAGCAT 
GCCTATGACT TTTACAAGCC TGACATGCTT TCAGAATATC CTATAGTAGA TGGCAAACTC 
TCCATACAGT GCTACCTCAG TGCACTAGAC CGCTGCTACT CTGTCTACCG CAAAAAGATC 
CGAGCCCAGT GGCAGAAAGA GGGAAATGAT AAAGATTTTA CCTTGAATGA TTTTGGTTTC 
ATGATCTTCC ACTCGCCCTA CTGTAAGCTG GTTCAGAAGT CTCTAGCTCG GATGTTGCTG 
AATGACTTCC TTAATGACCA GAACAGAGAT AAAAATAGTA TATATAGTGG CCTGGAAGCC 
TTTGGGGATG TTAAATTAGA AGATACCTAC TTTGATAGAG ATGTGGAAAA GGCATTTATG 
AAGGCTAGTG CTGAACTCTT TAATCAGAAA ACAAAGGCAT CTTTGCTTGT GTCAAATCAA 
AATGGAAATA TGTACACATC GTCTGTTTAT GGCTCCCTCG CTTCTGTTCT AGCACAGTAC 
TCCCCTCAGC AGCTGGCAGG AAAGAGGATT GGTGTGTTCT CTTACGGTTC TGGTTTGGCT 
GCCACCCTGT ACTCCCTTAG AGTTACACAA GATGCCACAC CAGGGTCTGC TCTTGATAAA 
ATAACAGCAA GTTTATGTGA CCTCAAATCA AGACTTGACT CAAGAACTTG TGTGGCACCA 
GATGTCTTTG CTGAAAACAT GAAGCTCAGA GAGGACACTC ATCACTTGGT CAACTATATT 
CCTCAGAGTT CAGTAGATTC CCTCTTCGAA GGGACCTGGT ACCTCGTCAG AGTGGATGAG 
AAGCACAGGA GGACCTATGC CCGGCGCCCC TCCCCCAGTG AGGATACTCT GGGTGAAGGA 
GTAGGACTCG TACATTCAAG TGCAGCCACT GAGCATATCC CAAGTCCTGC TAAGAAAGTG 
CCAAGACTCC CTGCCACAGC AGCAGAAGCC GAAGCAGCTG TCATTAGTAA CGGGGAGCAT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HMGCS1 ( XM_005619344.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005619401.1 Canis lupus familiaris 3-hydroxy-3-methylglutaryl-CoA synthase 1 (HMGCS1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OCe05473
Clone ID Related Accession (Same CDS sequence)	XM_005619344.1 , XM_005619343.2 , XM_536483.4 , XM_005619344.2 , XM_536483.5
Accession Version	XM_005619344.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1563bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	hydroxymethylglutaryl-CoA synthase, cytoplasmic isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006586.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005619344.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGGGT CACTTCCTTT GAATGCAGAA GCCTGCTGGC CAAAAGATGT GGGAATTGTT 
GCCCTTGAGA TCTATTTTCC TTCTCAATAT GTTGATCAAG CAGAGTTGGA AAAATATGAT 
GGTGTAGATG CTGGAAAGTA TACTATTGGC TTGGGCCAGG CCAAGATGGG CTTCTGCACG 
GATAGAGAAG ATATCAACTC TCTTTGCCTG ACTGTGGTTC AGAACCTTAT GGAGAGACAT 
AGCCTTTCGT ATGATTGCAT TGGGCGTTTA GAAGTTGGAA CAGAGACAAT CATTGACAAA 
TCAAAATCCG TGAAGACTAA TTTGATGCAG CTCTTTGAGG AGTCTGGGAA CACAGATATA 
GAAGGAATAG ATACAACTAA TGCATGCTAT GGAGGCACAG CCGCTGTCTT CAATGCTGTT 
AATTGGATTG AGTCCAGCTC CTGGGATGGA CGGTATGCCC TGGTAGTTGC GGGAGATATT 
GCTGTATATG CCACAGGAAA TGCTAGACCT ACAGGTGGAG TGGGAGCTGT TGCTCTGCTA 
ATTGGGCCAA ATGCGCCTTT AATTTTTGAC CGAGGACTTC GTGGGACACA TATGCAGCAT 
GCCTATGACT TTTACAAGCC TGACATGCTT TCAGAATATC CTATAGTAGA TGGCAAACTC 
TCCATACAGT GCTACCTCAG TGCACTAGAC CGCTGCTACT CTGTCTACCG CAAAAAGATC 
CGAGCCCAGT GGCAGAAAGA GGGAAATGAT AAAGATTTTA CCTTGAATGA TTTTGGTTTC 
ATGATCTTCC ACTCGCCCTA CTGTAAGCTG GTTCAGAAGT CTCTAGCTCG GATGTTGCTG 
AATGACTTCC TTAATGACCA GAACAGAGAT AAAAATAGTA TATATAGTGG CCTGGAAGCC 
TTTGGGGATG TTAAATTAGA AGATACCTAC TTTGATAGAG ATGTGGAAAA GGCATTTATG 
AAGGCTAGTG CTGAACTCTT TAATCAGAAA ACAAAGGCAT CTTTGCTTGT GTCAAATCAA 
AATGGAAATA TGTACACATC GTCTGTTTAT GGCTCCCTCG CTTCTGTTCT AGCACAGTAC 
TCCCCTCAGC AGCTGGCAGG AAAGAGGATT GGTGTGTTCT CTTACGGTTC TGGTTTGGCT 
GCCACCCTGT ACTCCCTTAG AGTTACACAA GATGCCACAC CAGGGTCTGC TCTTGATAAA 
ATAACAGCAA GTTTATGTGA CCTCAAATCA AGACTTGACT CAAGAACTTG TGTGGCACCA 
GATGTCTTTG CTGAAAACAT GAAGCTCAGA GAGGACACTC ATCACTTGGT CAACTATATT 
CCTCAGAGTT CAGTAGATTC CCTCTTCGAA GGGACCTGGT ACCTCGTCAG AGTGGATGAG 
AAGCACAGGA GGACCTATGC CCGGCGCCCC TCCCCCAGTG AGGATACTCT GGGTGAAGGA 
GTAGGACTCG TACATTCAAG TGCAGCCACT GAGCATATCC CAAGTCCTGC TAAGAAAGTG 
CCAAGACTCC CTGCCACAGC AGCAGAAGCC GAAGCAGCTG TCATTAGTAA CGGGGAGCAT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005619401.1
CDS	164..1726
Translation	MPGSLPLNAEACWPKDVGIVALEIYFPSQYVDQAELEKYDGVDAGKYTIGLGQAKMGFCTDREDINSLCLTVVQNLMERHSLSYDCIGRLEVGTETIIDKSKSVKTNLMQLFEESGNTDIEGIDTTNACYGGTAAVFNAVNWIESSSWDGRYALVVAGDIAVYATGNARPTGGVGAVALLIGPNAPLIFDRGLRGTHMQHAYDFYKPDMLSEYPIVDGKLSIQCYLSALDRCYSVYRKKIRAQWQKEGNDKDFTLNDFGFMIFHSPYCKLVQKSLARMLLNDFLNDQNRDKNSIYSGLEAFGDVKLEDTYFDRDVEKAFMKASAELFNQKTKASLLVSNQNGNMYTSSVYGSLASVLAQYSPQQLAGKRIGVFSYGSGLAATLYSLRVTQDATPGSALDKITASLCDLKSRLDSRTCVAPDVFAENMKLREDTHHLVNYIPQSSVDSLFEGTWYLVRVDEKHRRTYARRPSPSEDTLGEGVGLVHSSAATEHIPSPAKKVPRLPATAAEAEAAVISNGEH

Target ORF information:

RefSeq Version	XM_005619344.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris 3-hydroxy-3-methylglutaryl-CoA synthase 1 (HMGCS1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005619344.2

ORF Insert Sequence:

ATGCCTGGGT CACTTCCTTT GAATGCAGAA GCCTGCTGGC CAAAAGATGT GGGAATTGTT 
GCCCTTGAGA TCTATTTTCC TTCTCAATAT GTTGATCAAG CAGAGTTGGA AAAATATGAT 
GGTGTAGATG CTGGAAAGTA TACTATTGGC TTGGGCCAGG CCAAGATGGG CTTCTGCACG 
GATAGAGAAG ATATCAACTC TCTTTGCCTG ACTGTGGTTC AGAACCTTAT GGAGAGACAT 
AGCCTTTCGT ATGATTGCAT TGGGCGTTTA GAAGTTGGAA CAGAGACAAT CATTGACAAA 
TCAAAATCCG TGAAGACTAA TTTGATGCAG CTCTTTGAGG AGTCTGGGAA CACAGATATA 
GAAGGAATAG ATACAACTAA TGCATGCTAT GGAGGCACAG CCGCTGTCTT CAATGCTGTT 
AATTGGATTG AGTCCAGCTC CTGGGATGGA CGGTATGCCC TGGTAGTTGC GGGAGATATT 
GCTGTATATG CCACAGGAAA TGCTAGACCT ACAGGTGGAG TGGGAGCTGT TGCTCTGCTA 
ATTGGGCCAA ATGCGCCTTT AATTTTTGAC CGAGGACTTC GTGGGACACA TATGCAGCAT 
GCCTATGACT TTTACAAGCC TGACATGCTT TCAGAATATC CTATAGTAGA TGGCAAACTC 
TCCATACAGT GCTACCTCAG TGCACTAGAC CGCTGCTACT CTGTCTACCG CAAAAAGATC 
CGAGCCCAGT GGCAGAAAGA GGGAAATGAT AAAGATTTTA CCTTGAATGA TTTTGGTTTC 
ATGATCTTCC ACTCGCCCTA CTGTAAGCTG GTTCAGAAGT CTCTAGCTCG GATGTTGCTG 
AATGACTTCC TTAATGACCA GAACAGAGAT AAAAATAGTA TATATAGTGG CCTGGAAGCC 
TTTGGGGATG TTAAATTAGA AGATACCTAC TTTGATAGAG ATGTGGAAAA GGCATTTATG 
AAGGCTAGTG CTGAACTCTT TAATCAGAAA ACAAAGGCAT CTTTGCTTGT GTCAAATCAA 
AATGGAAATA TGTACACATC GTCTGTTTAT GGCTCCCTCG CTTCTGTTCT AGCACAGTAC 
TCCCCTCAGC AGCTGGCAGG AAAGAGGATT GGTGTGTTCT CTTACGGTTC TGGTTTGGCT 
GCCACCCTGT ACTCCCTTAG AGTTACACAA GATGCCACAC CAGGGTCTGC TCTTGATAAA 
ATAACAGCAA GTTTATGTGA CCTCAAATCA AGACTTGACT CAAGAACTTG TGTGGCACCA 
GATGTCTTTG CTGAAAACAT GAAGCTCAGA GAGGACACTC ATCACTTGGT CAACTATATT 
CCTCAGAGTT CAGTAGATTC CCTCTTCGAA GGGACCTGGT ACCTCGTCAG AGTGGATGAG 
AAGCACAGGA GGACCTATGC CCGGCGCCCC TCCCCCAGTG AGGATACTCT GGGTGAAGGA 
GTAGGACTCG TACATTCAAG TGCAGCCACT GAGCATATCC CAAGTCCTGC TAAGAAAGTG 
CCAAGACTCC CTGCCACAGC AGCAGAAGCC GAAGCAGCTG TCATTAGTAA CGGGGAGCAT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HMGCS1 ( XM_536483.5 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_536483.3 Canis lupus familiaris 3-hydroxy-3-methylglutaryl-CoA synthase 1 (HMGCS1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OCe05473
Clone ID Related Accession (Same CDS sequence)	XM_005619344.1 , XM_005619343.2 , XM_536483.4 , XM_005619344.2 , XM_536483.5
Accession Version	XM_536483.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1563bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	hydroxymethylglutaryl-CoA synthase, cytoplasmic isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006586.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_536483.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGGGT CACTTCCTTT GAATGCAGAA GCCTGCTGGC CAAAAGATGT GGGAATTGTT 
GCCCTTGAGA TCTATTTTCC TTCTCAATAT GTTGATCAAG CAGAGTTGGA AAAATATGAT 
GGTGTAGATG CTGGAAAGTA TACTATTGGC TTGGGCCAGG CCAAGATGGG CTTCTGCACG 
GATAGAGAAG ATATCAACTC TCTTTGCCTG ACTGTGGTTC AGAACCTTAT GGAGAGACAT 
AGCCTTTCGT ATGATTGCAT TGGGCGTTTA GAAGTTGGAA CAGAGACAAT CATTGACAAA 
TCAAAATCCG TGAAGACTAA TTTGATGCAG CTCTTTGAGG AGTCTGGGAA CACAGATATA 
GAAGGAATAG ATACAACTAA TGCATGCTAT GGAGGCACAG CCGCTGTCTT CAATGCTGTT 
AATTGGATTG AGTCCAGCTC CTGGGATGGA CGGTATGCCC TGGTAGTTGC GGGAGATATT 
GCTGTATATG CCACAGGAAA TGCTAGACCT ACAGGTGGAG TGGGAGCTGT TGCTCTGCTA 
ATTGGGCCAA ATGCGCCTTT AATTTTTGAC CGAGGACTTC GTGGGACACA TATGCAGCAT 
GCCTATGACT TTTACAAGCC TGACATGCTT TCAGAATATC CTATAGTAGA TGGCAAACTC 
TCCATACAGT GCTACCTCAG TGCACTAGAC CGCTGCTACT CTGTCTACCG CAAAAAGATC 
CGAGCCCAGT GGCAGAAAGA GGGAAATGAT AAAGATTTTA CCTTGAATGA TTTTGGTTTC 
ATGATCTTCC ACTCGCCCTA CTGTAAGCTG GTTCAGAAGT CTCTAGCTCG GATGTTGCTG 
AATGACTTCC TTAATGACCA GAACAGAGAT AAAAATAGTA TATATAGTGG CCTGGAAGCC 
TTTGGGGATG TTAAATTAGA AGATACCTAC TTTGATAGAG ATGTGGAAAA GGCATTTATG 
AAGGCTAGTG CTGAACTCTT TAATCAGAAA ACAAAGGCAT CTTTGCTTGT GTCAAATCAA 
AATGGAAATA TGTACACATC GTCTGTTTAT GGCTCCCTCG CTTCTGTTCT AGCACAGTAC 
TCCCCTCAGC AGCTGGCAGG AAAGAGGATT GGTGTGTTCT CTTACGGTTC TGGTTTGGCT 
GCCACCCTGT ACTCCCTTAG AGTTACACAA GATGCCACAC CAGGGTCTGC TCTTGATAAA 
ATAACAGCAA GTTTATGTGA CCTCAAATCA AGACTTGACT CAAGAACTTG TGTGGCACCA 
GATGTCTTTG CTGAAAACAT GAAGCTCAGA GAGGACACTC ATCACTTGGT CAACTATATT 
CCTCAGAGTT CAGTAGATTC CCTCTTCGAA GGGACCTGGT ACCTCGTCAG AGTGGATGAG 
AAGCACAGGA GGACCTATGC CCGGCGCCCC TCCCCCAGTG AGGATACTCT GGGTGAAGGA 
GTAGGACTCG TACATTCAAG TGCAGCCACT GAGCATATCC CAAGTCCTGC TAAGAAAGTG 
CCAAGACTCC CTGCCACAGC AGCAGAAGCC GAAGCAGCTG TCATTAGTAA CGGGGAGCAT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_536483.3
CDS	227..1789
Translation	MPGSLPLNAEACWPKDVGIVALEIYFPSQYVDQAELEKYDGVDAGKYTIGLGQAKMGFCTDREDINSLCLTVVQNLMERHSLSYDCIGRLEVGTETIIDKSKSVKTNLMQLFEESGNTDIEGIDTTNACYGGTAAVFNAVNWIESSSWDGRYALVVAGDIAVYATGNARPTGGVGAVALLIGPNAPLIFDRGLRGTHMQHAYDFYKPDMLSEYPIVDGKLSIQCYLSALDRCYSVYRKKIRAQWQKEGNDKDFTLNDFGFMIFHSPYCKLVQKSLARMLLNDFLNDQNRDKNSIYSGLEAFGDVKLEDTYFDRDVEKAFMKASAELFNQKTKASLLVSNQNGNMYTSSVYGSLASVLAQYSPQQLAGKRIGVFSYGSGLAATLYSLRVTQDATPGSALDKITASLCDLKSRLDSRTCVAPDVFAENMKLREDTHHLVNYIPQSSVDSLFEGTWYLVRVDEKHRRTYARRPSPSEDTLGEGVGLVHSSAATEHIPSPAKKVPRLPATAAEAEAAVISNGEH

Target ORF information:

RefSeq Version	XM_536483.5
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris 3-hydroxy-3-methylglutaryl-CoA synthase 1 (HMGCS1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_536483.5

ORF Insert Sequence:

ATGCCTGGGT CACTTCCTTT GAATGCAGAA GCCTGCTGGC CAAAAGATGT GGGAATTGTT 
GCCCTTGAGA TCTATTTTCC TTCTCAATAT GTTGATCAAG CAGAGTTGGA AAAATATGAT 
GGTGTAGATG CTGGAAAGTA TACTATTGGC TTGGGCCAGG CCAAGATGGG CTTCTGCACG 
GATAGAGAAG ATATCAACTC TCTTTGCCTG ACTGTGGTTC AGAACCTTAT GGAGAGACAT 
AGCCTTTCGT ATGATTGCAT TGGGCGTTTA GAAGTTGGAA CAGAGACAAT CATTGACAAA 
TCAAAATCCG TGAAGACTAA TTTGATGCAG CTCTTTGAGG AGTCTGGGAA CACAGATATA 
GAAGGAATAG ATACAACTAA TGCATGCTAT GGAGGCACAG CCGCTGTCTT CAATGCTGTT 
AATTGGATTG AGTCCAGCTC CTGGGATGGA CGGTATGCCC TGGTAGTTGC GGGAGATATT 
GCTGTATATG CCACAGGAAA TGCTAGACCT ACAGGTGGAG TGGGAGCTGT TGCTCTGCTA 
ATTGGGCCAA ATGCGCCTTT AATTTTTGAC CGAGGACTTC GTGGGACACA TATGCAGCAT 
GCCTATGACT TTTACAAGCC TGACATGCTT TCAGAATATC CTATAGTAGA TGGCAAACTC 
TCCATACAGT GCTACCTCAG TGCACTAGAC CGCTGCTACT CTGTCTACCG CAAAAAGATC 
CGAGCCCAGT GGCAGAAAGA GGGAAATGAT AAAGATTTTA CCTTGAATGA TTTTGGTTTC 
ATGATCTTCC ACTCGCCCTA CTGTAAGCTG GTTCAGAAGT CTCTAGCTCG GATGTTGCTG 
AATGACTTCC TTAATGACCA GAACAGAGAT AAAAATAGTA TATATAGTGG CCTGGAAGCC 
TTTGGGGATG TTAAATTAGA AGATACCTAC TTTGATAGAG ATGTGGAAAA GGCATTTATG 
AAGGCTAGTG CTGAACTCTT TAATCAGAAA ACAAAGGCAT CTTTGCTTGT GTCAAATCAA 
AATGGAAATA TGTACACATC GTCTGTTTAT GGCTCCCTCG CTTCTGTTCT AGCACAGTAC 
TCCCCTCAGC AGCTGGCAGG AAAGAGGATT GGTGTGTTCT CTTACGGTTC TGGTTTGGCT 
GCCACCCTGT ACTCCCTTAG AGTTACACAA GATGCCACAC CAGGGTCTGC TCTTGATAAA 
ATAACAGCAA GTTTATGTGA CCTCAAATCA AGACTTGACT CAAGAACTTG TGTGGCACCA 
GATGTCTTTG CTGAAAACAT GAAGCTCAGA GAGGACACTC ATCACTTGGT CAACTATATT 
CCTCAGAGTT CAGTAGATTC CCTCTTCGAA GGGACCTGGT ACCTCGTCAG AGTGGATGAG 
AAGCACAGGA GGACCTATGC CCGGCGCCCC TCCCCCAGTG AGGATACTCT GGGTGAAGGA 
GTAGGACTCG TACATTCAAG TGCAGCCACT GAGCATATCC CAAGTCCTGC TAAGAAAGTG 
CCAAGACTCC CTGCCACAGC AGCAGAAGCC GAAGCAGCTG TCATTAGTAA CGGGGAGCAT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HMGCS1 ( XM_022417862.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022273570.1 Canis lupus familiaris 3-hydroxy-3-methylglutaryl-CoA synthase 1 (HMGCS1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe287715
Clone ID Related Accession (Same CDS sequence)	XM_022417862.1 , XM_022417863.1
Accession Version	XM_022417862.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1689bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	hydroxymethylglutaryl-CoA synthase, cytoplasmic isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006586.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTAGAGA AAAGGATTAC TCTGAGCTTC CCTGGAGGAG GAATGTGGAC AACAGTGCCT 
GGAGGAGGAA ACAGAGGGAC AGACCTGGAG ACTGCAGTTC TTTATTCCTC ACACAGCTCT 
TTCACCATGC CTGGGTCACT TCCTTTGAAT GCAGAAGCCT GCTGGCCAAA AGATGTGGGA 
ATTGTTGCCC TTGAGATCTA TTTTCCTTCT CAATATGTTG ATCAAGCAGA GTTGGAAAAA 
TATGATGGTG TAGATGCTGG AAAGTATACT ATTGGCTTGG GCCAGGCCAA GATGGGCTTC 
TGCACGGATA GAGAAGATAT CAACTCTCTT TGCCTGACTG TGGTTCAGAA CCTTATGGAG 
AGACATAGCC TTTCGTATGA TTGCATTGGG CGTTTAGAAG TTGGAACAGA GACAATCATT 
GACAAATCAA AATCCGTGAA GACTAATTTG ATGCAGCTCT TTGAGGAGTC TGGGAACACA 
GATATAGAAG GAATAGATAC AACTAATGCA TGCTATGGAG GCACAGCCGC TGTCTTCAAT 
GCTGTTAATT GGATTGAGTC CAGCTCCTGG GATGGACGGT ATGCCCTGGT AGTTGCGGGA 
GATATTGCTG TATATGCCAC AGGAAATGCT AGACCTACAG GTGGAGTGGG AGCTGTTGCT 
CTGCTAATTG GGCCAAATGC GCCTTTAATT TTTGACCGAG GACTTCGTGG GACACATATG 
CAGCATGCCT ATGACTTTTA CAAGCCTGAC ATGCTTTCAG AATATCCTAT AGTAGATGGC 
AAACTCTCCA TACAGTGCTA CCTCAGTGCA CTAGACCGCT GCTACTCTGT CTACCGCAAA 
AAGATCCGAG CCCAGTGGCA GAAAGAGGGA AATGATAAAG ATTTTACCTT GAATGATTTT 
GGTTTCATGA TCTTCCACTC GCCCTACTGT AAGCTGGTTC AGAAGTCTCT AGCTCGGATG 
TTGCTGAATG ACTTCCTTAA TGACCAGAAC AGAGATAAAA ATAGTATATA TAGTGGCCTG 
GAAGCCTTTG GGGATGTTAA ATTAGAAGAT ACCTACTTTG ATAGAGATGT GGAAAAGGCA 
TTTATGAAGG CTAGTGCTGA ACTCTTTAAT CAGAAAACAA AGGCATCTTT GCTTGTGTCA 
AATCAAAATG GAAATATGTA CACATCGTCT GTTTATGGCT CCCTCGCTTC TGTTCTAGCA 
CAGTACTCCC CTCAGCAGCT GGCAGGAAAG AGGATTGGTG TGTTCTCTTA CGGTTCTGGT 
TTGGCTGCCA CCCTGTACTC CCTTAGAGTT ACACAAGATG CCACACCAGG GTCTGCTCTT 
GATAAAATAA CAGCAAGTTT ATGTGACCTC AAATCAAGAC TTGACTCAAG AACTTGTGTG 
GCACCAGATG TCTTTGCTGA AAACATGAAG CTCAGAGAGG ACACTCATCA CTTGGTCAAC 
TATATTCCTC AGAGTTCAGT AGATTCCCTC TTCGAAGGGA CCTGGTACCT CGTCAGAGTG 
GATGAGAAGC ACAGGAGGAC CTATGCCCGG CGCCCCTCCC CCAGTGAGGA TACTCTGGGT 
GAAGGAGTAG GACTCGTACA TTCAAGTGCA GCCACTGAGC ATATCCCAAG TCCTGCTAAG 
AAAGTGCCAA GACTCCCTGC CACAGCAGCA GAAGCCGAAG CAGCTGTCAT TAGTAACGGG 
GAGCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022273570.1
CDS	120..1808
Translation	MVEKRITLSFPGGGMWTTVPGGGNRGTDLETAVLYSSHSSFTMPGSLPLNAEACWPKDVGIVALEIYFPSQYVDQAELEKYDGVDAGKYTIGLGQAKMGFCTDREDINSLCLTVVQNLMERHSLSYDCIGRLEVGTETIIDKSKSVKTNLMQLFEESGNTDIEGIDTTNACYGGTAAVFNAVNWIESSSWDGRYALVVAGDIAVYATGNARPTGGVGAVALLIGPNAPLIFDRGLRGTHMQHAYDFYKPDMLSEYPIVDGKLSIQCYLSALDRCYSVYRKKIRAQWQKEGNDKDFTLNDFGFMIFHSPYCKLVQKSLARMLLNDFLNDQNRDKNSIYSGLEAFGDVKLEDTYFDRDVEKAFMKASAELFNQKTKASLLVSNQNGNMYTSSVYGSLASVLAQYSPQQLAGKRIGVFSYGSGLAATLYSLRVTQDATPGSALDKITASLCDLKSRLDSRTCVAPDVFAENMKLREDTHHLVNYIPQSSVDSLFEGTWYLVRVDEKHRRTYARRPSPSEDTLGEGVGLVHSSAATEHIPSPAKKVPRLPATAAEAEAAVISNGEH

Target ORF information:

RefSeq Version	XM_022417862.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris 3-hydroxy-3-methylglutaryl-CoA synthase 1 (HMGCS1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022417862.1

ORF Insert Sequence:

ATGGTAGAGA AAAGGATTAC TCTGAGCTTC CCTGGAGGAG GAATGTGGAC AACAGTGCCT 
GGAGGAGGAA ACAGAGGGAC AGACCTGGAG ACTGCAGTTC TTTATTCCTC ACACAGCTCT 
TTCACCATGC CTGGGTCACT TCCTTTGAAT GCAGAAGCCT GCTGGCCAAA AGATGTGGGA 
ATTGTTGCCC TTGAGATCTA TTTTCCTTCT CAATATGTTG ATCAAGCAGA GTTGGAAAAA 
TATGATGGTG TAGATGCTGG AAAGTATACT ATTGGCTTGG GCCAGGCCAA GATGGGCTTC 
TGCACGGATA GAGAAGATAT CAACTCTCTT TGCCTGACTG TGGTTCAGAA CCTTATGGAG 
AGACATAGCC TTTCGTATGA TTGCATTGGG CGTTTAGAAG TTGGAACAGA GACAATCATT 
GACAAATCAA AATCCGTGAA GACTAATTTG ATGCAGCTCT TTGAGGAGTC TGGGAACACA 
GATATAGAAG GAATAGATAC AACTAATGCA TGCTATGGAG GCACAGCCGC TGTCTTCAAT 
GCTGTTAATT GGATTGAGTC CAGCTCCTGG GATGGACGGT ATGCCCTGGT AGTTGCGGGA 
GATATTGCTG TATATGCCAC AGGAAATGCT AGACCTACAG GTGGAGTGGG AGCTGTTGCT 
CTGCTAATTG GGCCAAATGC GCCTTTAATT TTTGACCGAG GACTTCGTGG GACACATATG 
CAGCATGCCT ATGACTTTTA CAAGCCTGAC ATGCTTTCAG AATATCCTAT AGTAGATGGC 
AAACTCTCCA TACAGTGCTA CCTCAGTGCA CTAGACCGCT GCTACTCTGT CTACCGCAAA 
AAGATCCGAG CCCAGTGGCA GAAAGAGGGA AATGATAAAG ATTTTACCTT GAATGATTTT 
GGTTTCATGA TCTTCCACTC GCCCTACTGT AAGCTGGTTC AGAAGTCTCT AGCTCGGATG 
TTGCTGAATG ACTTCCTTAA TGACCAGAAC AGAGATAAAA ATAGTATATA TAGTGGCCTG 
GAAGCCTTTG GGGATGTTAA ATTAGAAGAT ACCTACTTTG ATAGAGATGT GGAAAAGGCA 
TTTATGAAGG CTAGTGCTGA ACTCTTTAAT CAGAAAACAA AGGCATCTTT GCTTGTGTCA 
AATCAAAATG GAAATATGTA CACATCGTCT GTTTATGGCT CCCTCGCTTC TGTTCTAGCA 
CAGTACTCCC CTCAGCAGCT GGCAGGAAAG AGGATTGGTG TGTTCTCTTA CGGTTCTGGT 
TTGGCTGCCA CCCTGTACTC CCTTAGAGTT ACACAAGATG CCACACCAGG GTCTGCTCTT 
GATAAAATAA CAGCAAGTTT ATGTGACCTC AAATCAAGAC TTGACTCAAG AACTTGTGTG 
GCACCAGATG TCTTTGCTGA AAACATGAAG CTCAGAGAGG ACACTCATCA CTTGGTCAAC 
TATATTCCTC AGAGTTCAGT AGATTCCCTC TTCGAAGGGA CCTGGTACCT CGTCAGAGTG 
GATGAGAAGC ACAGGAGGAC CTATGCCCGG CGCCCCTCCC CCAGTGAGGA TACTCTGGGT 
GAAGGAGTAG GACTCGTACA TTCAAGTGCA GCCACTGAGC ATATCCCAAG TCCTGCTAAG 
AAAGTGCCAA GACTCCCTGC CACAGCAGCA GAAGCCGAAG CAGCTGTCAT TAGTAACGGG 
GAGCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HMGCS1 ( XM_022417863.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022273571.1 Canis lupus familiaris 3-hydroxy-3-methylglutaryl-CoA synthase 1 (HMGCS1), transcript variant X2, mRNA.

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CloneID	OCe287715
Clone ID Related Accession (Same CDS sequence)	XM_022417862.1 , XM_022417863.1
Accession Version	XM_022417863.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1689bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	hydroxymethylglutaryl-CoA synthase, cytoplasmic isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006586.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTAGAGA AAAGGATTAC TCTGAGCTTC CCTGGAGGAG GAATGTGGAC AACAGTGCCT 
GGAGGAGGAA ACAGAGGGAC AGACCTGGAG ACTGCAGTTC TTTATTCCTC ACACAGCTCT 
TTCACCATGC CTGGGTCACT TCCTTTGAAT GCAGAAGCCT GCTGGCCAAA AGATGTGGGA 
ATTGTTGCCC TTGAGATCTA TTTTCCTTCT CAATATGTTG ATCAAGCAGA GTTGGAAAAA 
TATGATGGTG TAGATGCTGG AAAGTATACT ATTGGCTTGG GCCAGGCCAA GATGGGCTTC 
TGCACGGATA GAGAAGATAT CAACTCTCTT TGCCTGACTG TGGTTCAGAA CCTTATGGAG 
AGACATAGCC TTTCGTATGA TTGCATTGGG CGTTTAGAAG TTGGAACAGA GACAATCATT 
GACAAATCAA AATCCGTGAA GACTAATTTG ATGCAGCTCT TTGAGGAGTC TGGGAACACA 
GATATAGAAG GAATAGATAC AACTAATGCA TGCTATGGAG GCACAGCCGC TGTCTTCAAT 
GCTGTTAATT GGATTGAGTC CAGCTCCTGG GATGGACGGT ATGCCCTGGT AGTTGCGGGA 
GATATTGCTG TATATGCCAC AGGAAATGCT AGACCTACAG GTGGAGTGGG AGCTGTTGCT 
CTGCTAATTG GGCCAAATGC GCCTTTAATT TTTGACCGAG GACTTCGTGG GACACATATG 
CAGCATGCCT ATGACTTTTA CAAGCCTGAC ATGCTTTCAG AATATCCTAT AGTAGATGGC 
AAACTCTCCA TACAGTGCTA CCTCAGTGCA CTAGACCGCT GCTACTCTGT CTACCGCAAA 
AAGATCCGAG CCCAGTGGCA GAAAGAGGGA AATGATAAAG ATTTTACCTT GAATGATTTT 
GGTTTCATGA TCTTCCACTC GCCCTACTGT AAGCTGGTTC AGAAGTCTCT AGCTCGGATG 
TTGCTGAATG ACTTCCTTAA TGACCAGAAC AGAGATAAAA ATAGTATATA TAGTGGCCTG 
GAAGCCTTTG GGGATGTTAA ATTAGAAGAT ACCTACTTTG ATAGAGATGT GGAAAAGGCA 
TTTATGAAGG CTAGTGCTGA ACTCTTTAAT CAGAAAACAA AGGCATCTTT GCTTGTGTCA 
AATCAAAATG GAAATATGTA CACATCGTCT GTTTATGGCT CCCTCGCTTC TGTTCTAGCA 
CAGTACTCCC CTCAGCAGCT GGCAGGAAAG AGGATTGGTG TGTTCTCTTA CGGTTCTGGT 
TTGGCTGCCA CCCTGTACTC CCTTAGAGTT ACACAAGATG CCACACCAGG GTCTGCTCTT 
GATAAAATAA CAGCAAGTTT ATGTGACCTC AAATCAAGAC TTGACTCAAG AACTTGTGTG 
GCACCAGATG TCTTTGCTGA AAACATGAAG CTCAGAGAGG ACACTCATCA CTTGGTCAAC 
TATATTCCTC AGAGTTCAGT AGATTCCCTC TTCGAAGGGA CCTGGTACCT CGTCAGAGTG 
GATGAGAAGC ACAGGAGGAC CTATGCCCGG CGCCCCTCCC CCAGTGAGGA TACTCTGGGT 
GAAGGAGTAG GACTCGTACA TTCAAGTGCA GCCACTGAGC ATATCCCAAG TCCTGCTAAG 
AAAGTGCCAA GACTCCCTGC CACAGCAGCA GAAGCCGAAG CAGCTGTCAT TAGTAACGGG 
GAGCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022273571.1
CDS	120..1808
Translation	MVEKRITLSFPGGGMWTTVPGGGNRGTDLETAVLYSSHSSFTMPGSLPLNAEACWPKDVGIVALEIYFPSQYVDQAELEKYDGVDAGKYTIGLGQAKMGFCTDREDINSLCLTVVQNLMERHSLSYDCIGRLEVGTETIIDKSKSVKTNLMQLFEESGNTDIEGIDTTNACYGGTAAVFNAVNWIESSSWDGRYALVVAGDIAVYATGNARPTGGVGAVALLIGPNAPLIFDRGLRGTHMQHAYDFYKPDMLSEYPIVDGKLSIQCYLSALDRCYSVYRKKIRAQWQKEGNDKDFTLNDFGFMIFHSPYCKLVQKSLARMLLNDFLNDQNRDKNSIYSGLEAFGDVKLEDTYFDRDVEKAFMKASAELFNQKTKASLLVSNQNGNMYTSSVYGSLASVLAQYSPQQLAGKRIGVFSYGSGLAATLYSLRVTQDATPGSALDKITASLCDLKSRLDSRTCVAPDVFAENMKLREDTHHLVNYIPQSSVDSLFEGTWYLVRVDEKHRRTYARRPSPSEDTLGEGVGLVHSSAATEHIPSPAKKVPRLPATAAEAEAAVISNGEH

Target ORF information:

RefSeq Version	XM_022417863.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris 3-hydroxy-3-methylglutaryl-CoA synthase 1 (HMGCS1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022417863.1

ORF Insert Sequence:

ATGGTAGAGA AAAGGATTAC TCTGAGCTTC CCTGGAGGAG GAATGTGGAC AACAGTGCCT 
GGAGGAGGAA ACAGAGGGAC AGACCTGGAG ACTGCAGTTC TTTATTCCTC ACACAGCTCT 
TTCACCATGC CTGGGTCACT TCCTTTGAAT GCAGAAGCCT GCTGGCCAAA AGATGTGGGA 
ATTGTTGCCC TTGAGATCTA TTTTCCTTCT CAATATGTTG ATCAAGCAGA GTTGGAAAAA 
TATGATGGTG TAGATGCTGG AAAGTATACT ATTGGCTTGG GCCAGGCCAA GATGGGCTTC 
TGCACGGATA GAGAAGATAT CAACTCTCTT TGCCTGACTG TGGTTCAGAA CCTTATGGAG 
AGACATAGCC TTTCGTATGA TTGCATTGGG CGTTTAGAAG TTGGAACAGA GACAATCATT 
GACAAATCAA AATCCGTGAA GACTAATTTG ATGCAGCTCT TTGAGGAGTC TGGGAACACA 
GATATAGAAG GAATAGATAC AACTAATGCA TGCTATGGAG GCACAGCCGC TGTCTTCAAT 
GCTGTTAATT GGATTGAGTC CAGCTCCTGG GATGGACGGT ATGCCCTGGT AGTTGCGGGA 
GATATTGCTG TATATGCCAC AGGAAATGCT AGACCTACAG GTGGAGTGGG AGCTGTTGCT 
CTGCTAATTG GGCCAAATGC GCCTTTAATT TTTGACCGAG GACTTCGTGG GACACATATG 
CAGCATGCCT ATGACTTTTA CAAGCCTGAC ATGCTTTCAG AATATCCTAT AGTAGATGGC 
AAACTCTCCA TACAGTGCTA CCTCAGTGCA CTAGACCGCT GCTACTCTGT CTACCGCAAA 
AAGATCCGAG CCCAGTGGCA GAAAGAGGGA AATGATAAAG ATTTTACCTT GAATGATTTT 
GGTTTCATGA TCTTCCACTC GCCCTACTGT AAGCTGGTTC AGAAGTCTCT AGCTCGGATG 
TTGCTGAATG ACTTCCTTAA TGACCAGAAC AGAGATAAAA ATAGTATATA TAGTGGCCTG 
GAAGCCTTTG GGGATGTTAA ATTAGAAGAT ACCTACTTTG ATAGAGATGT GGAAAAGGCA 
TTTATGAAGG CTAGTGCTGA ACTCTTTAAT CAGAAAACAA AGGCATCTTT GCTTGTGTCA 
AATCAAAATG GAAATATGTA CACATCGTCT GTTTATGGCT CCCTCGCTTC TGTTCTAGCA 
CAGTACTCCC CTCAGCAGCT GGCAGGAAAG AGGATTGGTG TGTTCTCTTA CGGTTCTGGT 
TTGGCTGCCA CCCTGTACTC CCTTAGAGTT ACACAAGATG CCACACCAGG GTCTGCTCTT 
GATAAAATAA CAGCAAGTTT ATGTGACCTC AAATCAAGAC TTGACTCAAG AACTTGTGTG 
GCACCAGATG TCTTTGCTGA AAACATGAAG CTCAGAGAGG ACACTCATCA CTTGGTCAAC 
TATATTCCTC AGAGTTCAGT AGATTCCCTC TTCGAAGGGA CCTGGTACCT CGTCAGAGTG 
GATGAGAAGC ACAGGAGGAC CTATGCCCGG CGCCCCTCCC CCAGTGAGGA TACTCTGGGT 
GAAGGAGTAG GACTCGTACA TTCAAGTGCA GCCACTGAGC ATATCCCAAG TCCTGCTAAG 
AAAGTGCCAA GACTCCCTGC CACAGCAGCA GAAGCCGAAG CAGCTGTCAT TAGTAACGGG 
GAGCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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HMGCS1 cDNA ORF clone, Canis lupus familiaris(dog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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