HMGCS1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	HMGCS1
Entrez Gene ID	3157
Full Name	3-hydroxy-3-methylglutaryl-CoA synthase 1
Synonyms	HMGCS
General protein information	Preferred Names 3-hydroxy-3-methylglutaryl-CoA synthase 1 Names hydroxymethylglutaryl-CoA synthase, cytoplasmic 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) synthase 3-hydroxy-3-methylglutaryl-CoA synthase 1 (soluble) 3-hydroxy-3-methylglutaryl-Coenzyme A synthase 1 (soluble)
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 5 Map Location: 5p12
Disorder MIM:	142940

mRNA and Protein(s)

mRNA	Protein	Name
NM_001324220.1	NP_001311149.1	hydroxymethylglutaryl-CoA synthase, cytoplasmic isoform 1
NM_001324222.2	NP_001311151.1	hydroxymethylglutaryl-CoA synthase, cytoplasmic isoform 2
NM_001330663.2	NP_001317592.1	hydroxymethylglutaryl-CoA synthase, cytoplasmic isoform 1
XM_011514036.3	XP_011512338.1	hydroxymethylglutaryl-CoA synthase, cytoplasmic isoform X1
NM_001324224.1	NP_001311153.1	hydroxymethylglutaryl-CoA synthase, cytoplasmic isoform 2
NM_002130.7	NP_002121.4	hydroxymethylglutaryl-CoA synthase, cytoplasmic isoform 1
NM_001324222.1	NP_001311151.1	hydroxymethylglutaryl-CoA synthase, cytoplasmic isoform 2
NM_001098272.3	NP_001091742.1	hydroxymethylglutaryl-CoA synthase, cytoplasmic isoform 1
NM_001324223.2	NP_001311152.1	hydroxymethylglutaryl-CoA synthase, cytoplasmic isoform 2
NM_001330663.1	NP_001317592.1	hydroxymethylglutaryl-CoA synthase, cytoplasmic isoform 1
NM_001324219.2	NP_001311148.1	hydroxymethylglutaryl-CoA synthase, cytoplasmic isoform 1
NM_001324224.2	NP_001311153.1	hydroxymethylglutaryl-CoA synthase, cytoplasmic isoform 2
NM_001364188.2	NP_001351117.1	hydroxymethylglutaryl-CoA synthase, cytoplasmic isoform 1
XM_024446038.1	XP_024301806.1	hydroxymethylglutaryl-CoA synthase, cytoplasmic isoform X1
NM_001324223.1	NP_001311152.1	hydroxymethylglutaryl-CoA synthase, cytoplasmic isoform 2
NM_001324219.1	NP_001311148.1	hydroxymethylglutaryl-CoA synthase, cytoplasmic isoform 1
NM_001364188.1	NP_001351117.1	hydroxymethylglutaryl-CoA synthase, cytoplasmic isoform 1
NM_001098272.2	NP_001091742.1	hydroxymethylglutaryl-CoA synthase, cytoplasmic isoform 1
XM_011514036.2	XP_011512338.1	hydroxymethylglutaryl-CoA synthase, cytoplasmic isoform X2
NM_002130.8	NP_002121.4	hydroxymethylglutaryl-CoA synthase, cytoplasmic isoform 1
NM_001324220.2	NP_001311149.1	hydroxymethylglutaryl-CoA synthase, cytoplasmic isoform 1

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Hmgcs1	NP_666054.2
Kluyveromyces lactis	KLLA0D10505g	XP_453529.2
Eremothecium gossypii	AGOS_ADL356C	NP_983739.1
Magnaporthe oryzae (rice blast fungus)	MGG_01026	XP_003717917.1
Neurospora crassa	NCU03922	XP_957561.1
Oryza sativa (rice)	Os08g0544900	NP_001062410.1
Canis lupus familiaris (dog)	HMGCS1	XP_005619400.1
Bos taurus (cattle)	HMGCS1	NP_001193507.1
Danio rerio (zebrafish)	hmgcs1	NP_957379.1
Arabidopsis thaliana (thale cress)	MVA1	NP_192919.1
Oryza sativa (rice)	Os03g0118800	NP_001048774.1
Oryza sativa (rice)	Os09g0521400	NP_001063706.1
	HMGCS1	XP_002804403.1
Saccharomyces cerevisiae (baker's yeast)	ERG13	NP_013580.1
Xenopus tropicalis (tropical clawed frog)	hmgcs1	NP_001120172.1
Homo sapiens (human)	HMGCS1	NP_001091742.1
Rattus norvegicus (Norway rat)	Hmgcs1	NP_058964.1
Pan troglodytes (chimpanzee)	HMGCS1	XP_003310783.1
Gallus gallus (chicken)	HMGCS1	NP_990742.1
Drosophila melanogaster (fruit fly)	Hmgs	NP_725570.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005847	XP_315872.3
Schizosaccharomyces pombe (fission yeast)	hcs1	NP_593859.1

Gene Ontology
Bibliography

Related articles in PubMed

HMG-CoA synthase 1 is a synthetic lethal partner of BRAF
Zhao L, Fan J, Xia S, Pan Y, Liu S, Qian G, Qian Z, Kang HB, Arbiser JL, Pollack BP, Kudchadkar RR, Lawson DH, Rossi M, Abdel-Wahab O, Merghoub T, Khoury HJ, Khuri FR, Boise LH, Lonial S, Chen F, Chen J, Lin R
The Journal of biological chemistry292(24)10142-10152(2017 06)

Crystal structures of human HMG-CoA synthase isoforms provide insights into inherited ketogenesis disorders and inhibitor design.
Shafqat N, Turnbull A, Zschocke J, Oppermann U, Yue WW
Journal of molecular biology398(4)497-506(2010 May)

Multiple genetic variants along candidate pathways influence plasma high-density lipoprotein cholesterol concentrations.
Lu Y, Doll? ME, Imholz S, van 't Slot R, Verschuren WM, Wijmenga C, Feskens EJ, Boer JM
Journal of lipid research49(12)2582-9(2008 Dec)

Transcriptional regulation of HMG-CoA synthase and HMG-CoA reductase genes by human ACBP.
Vock C, D?ring F, Nitz I
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology22(5-6)515-24(2008)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data suggest that HMGCS1 (HMG-CoA synthase 1) signals through ketogenesis/acetoacetate to promote cell proliferation and BRAF(V600E)-dependent MEK1 activation in BRAF(V600E)-positive melanoma and colon cancer cells; HMGCS1 co-localizes with HMGCL (HMG-CoA lyase) and BRAF(V600E) in cytosol of melanoma and colon cancer cells. (BRAF = proto-oncogene protein B-raf)
Title: HMG-CoA synthase 1 is a synthetic lethal partner of BRAF

The authors report high-resolution crystal structures of the human cytosolic (hHMGCS1) and mitochondrial (hHMGCS2) isoforms in binary product complexes.
Title: Crystal structures of human HMG-CoA synthase isoforms provide insights into inherited ketogenesis disorders and inhibitor design.

Observational study of gene-disease association. (HuGE Navigator)
Title: Multiple genetic variants along candidate pathways influence plasma high-density lipoprotein cholesterol concentrations.

Observational study of gene-disease association. (HuGE Navigator)
Title: Polymorphisms in the estrogen receptor 1 and vitamin C and matrix metalloproteinase gene families are associated with susceptibility to lymphoma.

ACBP is a transcriptional regulator of the HMGCS1 and HMGCR genes encoding rate-limiting enzymes of cholesterol synthesis pathway.
Title: Transcriptional regulation of HMG-CoA synthase and HMG-CoA reductase genes by human ACBP.

The following HMGCS1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HMGCS1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu01309	NM_001324220.2 Latest version!	Homo sapiens 3-hydroxy-3-methylglutaryl-CoA synthase 1 (HMGCS1), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu01309	NM_001324219.2 Latest version!	Homo sapiens 3-hydroxy-3-methylglutaryl-CoA synthase 1 (HMGCS1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu01309	NM_002130.8 Latest version!	Homo sapiens 3-hydroxy-3-methylglutaryl-CoA synthase 1 (HMGCS1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu01309	NM_001330663.2 Latest version!	Homo sapiens 3-hydroxy-3-methylglutaryl-CoA synthase 1 (HMGCS1), transcript variant 8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu01309	NM_001364188.2 Latest version!	Homo sapiens 3-hydroxy-3-methylglutaryl-CoA synthase 1 (HMGCS1), transcript variant 9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu01309	NM_001098272.3 Latest version!	Homo sapiens 3-hydroxy-3-methylglutaryl-CoA synthase 1 (HMGCS1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu39317	NM_001324224.2 Latest version!	Homo sapiens 3-hydroxy-3-methylglutaryl-CoA synthase 1 (HMGCS1), transcript variant 7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu39317	NM_001324223.2 Latest version!	Homo sapiens 3-hydroxy-3-methylglutaryl-CoA synthase 1 (HMGCS1), transcript variant 6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu39317	NM_001324222.2 Latest version!	Homo sapiens 3-hydroxy-3-methylglutaryl-CoA synthase 1 (HMGCS1), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu58268	XM_024446038.1 Latest version!	Homo sapiens 3-hydroxy-3-methylglutaryl-CoA synthase 1 (HMGCS1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OHu58268	XM_011514036.3 Latest version!	Homo sapiens 3-hydroxy-3-methylglutaryl-CoA synthase 1 (HMGCS1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
View Old Accession Versions >>
OHu39317	NM_001324222.1	Homo sapiens 3-hydroxy-3-methylglutaryl-CoA synthase 1 (HMGCS1), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu01309	NM_001330663.1	Homo sapiens 3-hydroxy-3-methylglutaryl-CoA synthase 1 (HMGCS1), transcript variant 8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu39317	NM_001324224.1	Homo sapiens 3-hydroxy-3-methylglutaryl-CoA synthase 1 (HMGCS1), transcript variant 7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu39317	NM_001324223.1	Homo sapiens 3-hydroxy-3-methylglutaryl-CoA synthase 1 (HMGCS1), transcript variant 6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu01309	NM_001324219.1	Homo sapiens 3-hydroxy-3-methylglutaryl-CoA synthase 1 (HMGCS1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu01309	NM_001364188.1	Homo sapiens 3-hydroxy-3-methylglutaryl-CoA synthase 1 (HMGCS1), transcript variant 9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu01309	NM_001098272.2	Homo sapiens 3-hydroxy-3-methylglutaryl-CoA synthase 1 (HMGCS1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu58268	XM_011514036.2	Homo sapiens 3-hydroxy-3-methylglutaryl-CoA synthase 1 (HMGCS1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OHu01309	NM_002130.7	Homo sapiens 3-hydroxy-3-methylglutaryl-CoA synthase 1 (HMGCS1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu01309	NM_001324220.1	Homo sapiens 3-hydroxy-3-methylglutaryl-CoA synthase 1 (HMGCS1), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HMGCS1 ( NM_001324220.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001311149.1 Homo sapiens 3-hydroxy-3-methylglutaryl-CoA synthase 1 (HMGCS1), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu01309
Clone ID Related Accession (Same CDS sequence)	NM_001324220.1 , NM_001330663.2 , NM_002130.7 , NM_001098272.3 , NM_001330663.1 , NM_001324219.2 , NM_001364188.2 , NM_001324219.1 , NM_001364188.1 , NM_001098272.2 , NM_002130.8 , NM_001324220.2
Accession Version	NM_001324220.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1563bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-24
Organism	Homo sapiens(human)
Product	hydroxymethylglutaryl-CoA synthase, cytoplasmic isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DB074885.1, BJ995153.1, AK315593.1 and AC114947.2. On Apr 28, 2016 this sequence version replaced XM_011514034.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.61106.1, SRR1803611.194174.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCTGGAT CACTTCCTTT GAATGCAGAA GCTTGCTGGC CAAAAGATGT GGGAATTGTT 
GCCCTTGAGA TCTATTTTCC TTCTCAATAT GTTGATCAAG CAGAGTTGGA AAAATATGAT 
GGTGTAGATG CTGGAAAGTA TACCATTGGC TTGGGCCAGG CCAAGATGGG CTTCTGCACA 
GATAGAGAAG ATATTAACTC TCTTTGCATG ACTGTGGTTC AGAATCTTAT GGAGAGAAAT 
AACCTTTCCT ATGATTGCAT TGGGCGGCTG GAAGTTGGAA CAGAGACAAT CATCGACAAA 
TCAAAGTCTG TGAAGACTAA TTTGATGCAG CTGTTTGAAG AGTCTGGGAA TACAGATATA 
GAAGGAATCG ACACAACTAA TGCATGCTAT GGAGGCACAG CTGCTGTCTT CAATGCTGTT 
AACTGGATTG AGTCCAGCTC TTGGGATGGA CGGTATGCCC TGGTAGTTGC AGGAGATATT 
GCTGTATATG CCACAGGAAA TGCTAGACCT ACAGGTGGAG TTGGAGCAGT AGCTCTGCTA 
ATTGGGCCAA ATGCTCCTTT AATTTTTGAA CGAGGGCTTC GTGGGACACA TATGCAACAT 
GCCTATGATT TTTACAAGCC TGATATGCTA TCTGAATATC CTATAGTAGA TGGAAAACTC 
TCCATACAGT GCTACCTCAG TGCATTAGAC CGCTGCTATT CTGTCTACTG CAAAAAGATC 
CATGCCCAGT GGCAGAAAGA GGGAAATGAT AAAGATTTTA CCTTGAATGA TTTTGGCTTC 
ATGATCTTTC ACTCACCATA TTGTAAACTG GTTCAGAAAT CTCTAGCTCG GATGTTGCTG 
AATGACTTCC TTAATGACCA GAATAGAGAT AAAAATAGTA TCTATAGTGG CCTGGAAGCC 
TTTGGGGATG TTAAATTAGA AGACACCTAC TTTGATAGAG ATGTGGAGAA GGCATTTATG 
AAGGCTAGCT CTGAACTCTT CAGTCAGAAA ACAAAGGCAT CTTTACTTGT ATCAAATCAA 
AATGGAAATA TGTACACATC TTCAGTATAT GGTTCCCTTG CATCTGTTCT AGCACAGTAC 
TCACCTCAGC AATTAGCAGG GAAGAGAATT GGAGTGTTTT CTTATGGTTC TGGTTTGGCT 
GCCACTCTGT ACTCTCTTAA AGTCACACAA GATGCTACAC CGGGGTCTGC TCTTGATAAA 
ATAACAGCAA GTTTATGTGA TCTTAAATCA AGGCTTGATT CAAGAACTGG TGTGGCACCA 
GATGTCTTCG CTGAAAACAT GAAGCTCAGA GAGGACACCC ATCATTTGGT CAACTATATT 
CCCCAGGGTT CAATAGATTC ACTCTTTGAA GGAACGTGGT ACTTAGTTAG GGTGGATGAA 
AAGCACAGAA GAACTTACGC TCGGCGTCCC ACTCCAAATG ATGACACTTT GGATGAAGGA 
GTAGGACTTG TGCATTCAAA CATAGCAACT GAGCATATTC CAAGCCCTGC CAAGAAAGTA 
CCAAGACTCC CTGCCACAGC AGCAGAACCT GAAGCAGCTG TCATTAGTAA TGGGGAACAT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001311149.1
CDS	247..1809
Translation	MPGSLPLNAEACWPKDVGIVALEIYFPSQYVDQAELEKYDGVDAGKYTIGLGQAKMGFCTDREDINSLCMTVVQNLMERNNLSYDCIGRLEVGTETIIDKSKSVKTNLMQLFEESGNTDIEGIDTTNACYGGTAAVFNAVNWIESSSWDGRYALVVAGDIAVYATGNARPTGGVGAVALLIGPNAPLIFERGLRGTHMQHAYDFYKPDMLSEYPIVDGKLSIQCYLSALDRCYSVYCKKIHAQWQKEGNDKDFTLNDFGFMIFHSPYCKLVQKSLARMLLNDFLNDQNRDKNSIYSGLEAFGDVKLEDTYFDRDVEKAFMKASSELFSQKTKASLLVSNQNGNMYTSSVYGSLASVLAQYSPQQLAGKRIGVFSYGSGLAATLYSLKVTQDATPGSALDKITASLCDLKSRLDSRTGVAPDVFAENMKLREDTHHLVNYIPQGSIDSLFEGTWYLVRVDEKHRRTYARRPTPNDDTLDEGVGLVHSNIATEHIPSPAKKVPRLPATAAEPEAAVISNGEH

Target ORF information:

RefSeq Version	NM_001324220.1
Organism	Homo sapiens(human)
Definition	Homo sapiens 3-hydroxy-3-methylglutaryl-CoA synthase 1 (HMGCS1), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001324220.1

ORF Insert Sequence:

ATGCCTGGAT CACTTCCTTT GAATGCAGAA GCTTGCTGGC CAAAAGATGT GGGAATTGTT 
GCCCTTGAGA TCTATTTTCC TTCTCAATAT GTTGATCAAG CAGAGTTGGA AAAATATGAT 
GGTGTAGATG CTGGAAAGTA TACCATTGGC TTGGGCCAGG CCAAGATGGG CTTCTGCACA 
GATAGAGAAG ATATTAACTC TCTTTGCATG ACTGTGGTTC AGAATCTTAT GGAGAGAAAT 
AACCTTTCCT ATGATTGCAT TGGGCGGCTG GAAGTTGGAA CAGAGACAAT CATCGACAAA 
TCAAAGTCTG TGAAGACTAA TTTGATGCAG CTGTTTGAAG AGTCTGGGAA TACAGATATA 
GAAGGAATCG ACACAACTAA TGCATGCTAT GGAGGCACAG CTGCTGTCTT CAATGCTGTT 
AACTGGATTG AGTCCAGCTC TTGGGATGGA CGGTATGCCC TGGTAGTTGC AGGAGATATT 
GCTGTATATG CCACAGGAAA TGCTAGACCT ACAGGTGGAG TTGGAGCAGT AGCTCTGCTA 
ATTGGGCCAA ATGCTCCTTT AATTTTTGAA CGAGGGCTTC GTGGGACACA TATGCAACAT 
GCCTATGATT TTTACAAGCC TGATATGCTA TCTGAATATC CTATAGTAGA TGGAAAACTC 
TCCATACAGT GCTACCTCAG TGCATTAGAC CGCTGCTATT CTGTCTACTG CAAAAAGATC 
CATGCCCAGT GGCAGAAAGA GGGAAATGAT AAAGATTTTA CCTTGAATGA TTTTGGCTTC 
ATGATCTTTC ACTCACCATA TTGTAAACTG GTTCAGAAAT CTCTAGCTCG GATGTTGCTG 
AATGACTTCC TTAATGACCA GAATAGAGAT AAAAATAGTA TCTATAGTGG CCTGGAAGCC 
TTTGGGGATG TTAAATTAGA AGACACCTAC TTTGATAGAG ATGTGGAGAA GGCATTTATG 
AAGGCTAGCT CTGAACTCTT CAGTCAGAAA ACAAAGGCAT CTTTACTTGT ATCAAATCAA 
AATGGAAATA TGTACACATC TTCAGTATAT GGTTCCCTTG CATCTGTTCT AGCACAGTAC 
TCACCTCAGC AATTAGCAGG GAAGAGAATT GGAGTGTTTT CTTATGGTTC TGGTTTGGCT 
GCCACTCTGT ACTCTCTTAA AGTCACACAA GATGCTACAC CGGGGTCTGC TCTTGATAAA 
ATAACAGCAA GTTTATGTGA TCTTAAATCA AGGCTTGATT CAAGAACTGG TGTGGCACCA 
GATGTCTTCG CTGAAAACAT GAAGCTCAGA GAGGACACCC ATCATTTGGT CAACTATATT 
CCCCAGGGTT CAATAGATTC ACTCTTTGAA GGAACGTGGT ACTTAGTTAG GGTGGATGAA 
AAGCACAGAA GAACTTACGC TCGGCGTCCC ACTCCAAATG ATGACACTTT GGATGAAGGA 
GTAGGACTTG TGCATTCAAA CATAGCAACT GAGCATATTC CAAGCCCTGC CAAGAAAGTA 
CCAAGACTCC CTGCCACAGC AGCAGAACCT GAAGCAGCTG TCATTAGTAA TGGGGAACAT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HMGCS1 ( NM_001330663.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001317592.1 Homo sapiens 3-hydroxy-3-methylglutaryl-CoA synthase 1 (HMGCS1), transcript variant 8, mRNA.

Price & Availability↑

CloneID	OHu01309
Clone ID Related Accession (Same CDS sequence)	NM_001324220.1 , NM_001330663.2 , NM_002130.7 , NM_001098272.3 , NM_001330663.1 , NM_001324219.2 , NM_001364188.2 , NM_001324219.1 , NM_001364188.1 , NM_001098272.2 , NM_002130.8 , NM_001324220.2
Accession Version	NM_001330663.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1563bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-27
Organism	Homo sapiens(human)
Product	hydroxymethylglutaryl-CoA synthase, cytoplasmic isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC114947.2. On May 31, 2019 this sequence version replaced NM_001330663.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.96054.1, SRR1660807.126299.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCTGGAT CACTTCCTTT GAATGCAGAA GCTTGCTGGC CAAAAGATGT GGGAATTGTT 
GCCCTTGAGA TCTATTTTCC TTCTCAATAT GTTGATCAAG CAGAGTTGGA AAAATATGAT 
GGTGTAGATG CTGGAAAGTA TACCATTGGC TTGGGCCAGG CCAAGATGGG CTTCTGCACA 
GATAGAGAAG ATATTAACTC TCTTTGCATG ACTGTGGTTC AGAATCTTAT GGAGAGAAAT 
AACCTTTCCT ATGATTGCAT TGGGCGGCTG GAAGTTGGAA CAGAGACAAT CATCGACAAA 
TCAAAGTCTG TGAAGACTAA TTTGATGCAG CTGTTTGAAG AGTCTGGGAA TACAGATATA 
GAAGGAATCG ACACAACTAA TGCATGCTAT GGAGGCACAG CTGCTGTCTT CAATGCTGTT 
AACTGGATTG AGTCCAGCTC TTGGGATGGA CGGTATGCCC TGGTAGTTGC AGGAGATATT 
GCTGTATATG CCACAGGAAA TGCTAGACCT ACAGGTGGAG TTGGAGCAGT AGCTCTGCTA 
ATTGGGCCAA ATGCTCCTTT AATTTTTGAA CGAGGGCTTC GTGGGACACA TATGCAACAT 
GCCTATGATT TTTACAAGCC TGATATGCTA TCTGAATATC CTATAGTAGA TGGAAAACTC 
TCCATACAGT GCTACCTCAG TGCATTAGAC CGCTGCTATT CTGTCTACTG CAAAAAGATC 
CATGCCCAGT GGCAGAAAGA GGGAAATGAT AAAGATTTTA CCTTGAATGA TTTTGGCTTC 
ATGATCTTTC ACTCACCATA TTGTAAACTG GTTCAGAAAT CTCTAGCTCG GATGTTGCTG 
AATGACTTCC TTAATGACCA GAATAGAGAT AAAAATAGTA TCTATAGTGG CCTGGAAGCC 
TTTGGGGATG TTAAATTAGA AGACACCTAC TTTGATAGAG ATGTGGAGAA GGCATTTATG 
AAGGCTAGCT CTGAACTCTT CAGTCAGAAA ACAAAGGCAT CTTTACTTGT ATCAAATCAA 
AATGGAAATA TGTACACATC TTCAGTATAT GGTTCCCTTG CATCTGTTCT AGCACAGTAC 
TCACCTCAGC AATTAGCAGG GAAGAGAATT GGAGTGTTTT CTTATGGTTC TGGTTTGGCT 
GCCACTCTGT ACTCTCTTAA AGTCACACAA GATGCTACAC CGGGGTCTGC TCTTGATAAA 
ATAACAGCAA GTTTATGTGA TCTTAAATCA AGGCTTGATT CAAGAACTGG TGTGGCACCA 
GATGTCTTCG CTGAAAACAT GAAGCTCAGA GAGGACACCC ATCATTTGGT CAACTATATT 
CCCCAGGGTT CAATAGATTC ACTCTTTGAA GGAACGTGGT ACTTAGTTAG GGTGGATGAA 
AAGCACAGAA GAACTTACGC TCGGCGTCCC ACTCCAAATG ATGACACTTT GGATGAAGGA 
GTAGGACTTG TGCATTCAAA CATAGCAACT GAGCATATTC CAAGCCCTGC CAAGAAAGTA 
CCAAGACTCC CTGCCACAGC AGCAGAACCT GAAGCAGCTG TCATTAGTAA TGGGGAACAT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001317592.1
CDS	230..1792
Translation	MPGSLPLNAEACWPKDVGIVALEIYFPSQYVDQAELEKYDGVDAGKYTIGLGQAKMGFCTDREDINSLCMTVVQNLMERNNLSYDCIGRLEVGTETIIDKSKSVKTNLMQLFEESGNTDIEGIDTTNACYGGTAAVFNAVNWIESSSWDGRYALVVAGDIAVYATGNARPTGGVGAVALLIGPNAPLIFERGLRGTHMQHAYDFYKPDMLSEYPIVDGKLSIQCYLSALDRCYSVYCKKIHAQWQKEGNDKDFTLNDFGFMIFHSPYCKLVQKSLARMLLNDFLNDQNRDKNSIYSGLEAFGDVKLEDTYFDRDVEKAFMKASSELFSQKTKASLLVSNQNGNMYTSSVYGSLASVLAQYSPQQLAGKRIGVFSYGSGLAATLYSLKVTQDATPGSALDKITASLCDLKSRLDSRTGVAPDVFAENMKLREDTHHLVNYIPQGSIDSLFEGTWYLVRVDEKHRRTYARRPTPNDDTLDEGVGLVHSNIATEHIPSPAKKVPRLPATAAEPEAAVISNGEH

Target ORF information:

RefSeq Version	NM_001330663.2
Organism	Homo sapiens(human)
Definition	Homo sapiens 3-hydroxy-3-methylglutaryl-CoA synthase 1 (HMGCS1), transcript variant 8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001330663.2

ORF Insert Sequence:

ATGCCTGGAT CACTTCCTTT GAATGCAGAA GCTTGCTGGC CAAAAGATGT GGGAATTGTT 
GCCCTTGAGA TCTATTTTCC TTCTCAATAT GTTGATCAAG CAGAGTTGGA AAAATATGAT 
GGTGTAGATG CTGGAAAGTA TACCATTGGC TTGGGCCAGG CCAAGATGGG CTTCTGCACA 
GATAGAGAAG ATATTAACTC TCTTTGCATG ACTGTGGTTC AGAATCTTAT GGAGAGAAAT 
AACCTTTCCT ATGATTGCAT TGGGCGGCTG GAAGTTGGAA CAGAGACAAT CATCGACAAA 
TCAAAGTCTG TGAAGACTAA TTTGATGCAG CTGTTTGAAG AGTCTGGGAA TACAGATATA 
GAAGGAATCG ACACAACTAA TGCATGCTAT GGAGGCACAG CTGCTGTCTT CAATGCTGTT 
AACTGGATTG AGTCCAGCTC TTGGGATGGA CGGTATGCCC TGGTAGTTGC AGGAGATATT 
GCTGTATATG CCACAGGAAA TGCTAGACCT ACAGGTGGAG TTGGAGCAGT AGCTCTGCTA 
ATTGGGCCAA ATGCTCCTTT AATTTTTGAA CGAGGGCTTC GTGGGACACA TATGCAACAT 
GCCTATGATT TTTACAAGCC TGATATGCTA TCTGAATATC CTATAGTAGA TGGAAAACTC 
TCCATACAGT GCTACCTCAG TGCATTAGAC CGCTGCTATT CTGTCTACTG CAAAAAGATC 
CATGCCCAGT GGCAGAAAGA GGGAAATGAT AAAGATTTTA CCTTGAATGA TTTTGGCTTC 
ATGATCTTTC ACTCACCATA TTGTAAACTG GTTCAGAAAT CTCTAGCTCG GATGTTGCTG 
AATGACTTCC TTAATGACCA GAATAGAGAT AAAAATAGTA TCTATAGTGG CCTGGAAGCC 
TTTGGGGATG TTAAATTAGA AGACACCTAC TTTGATAGAG ATGTGGAGAA GGCATTTATG 
AAGGCTAGCT CTGAACTCTT CAGTCAGAAA ACAAAGGCAT CTTTACTTGT ATCAAATCAA 
AATGGAAATA TGTACACATC TTCAGTATAT GGTTCCCTTG CATCTGTTCT AGCACAGTAC 
TCACCTCAGC AATTAGCAGG GAAGAGAATT GGAGTGTTTT CTTATGGTTC TGGTTTGGCT 
GCCACTCTGT ACTCTCTTAA AGTCACACAA GATGCTACAC CGGGGTCTGC TCTTGATAAA 
ATAACAGCAA GTTTATGTGA TCTTAAATCA AGGCTTGATT CAAGAACTGG TGTGGCACCA 
GATGTCTTCG CTGAAAACAT GAAGCTCAGA GAGGACACCC ATCATTTGGT CAACTATATT 
CCCCAGGGTT CAATAGATTC ACTCTTTGAA GGAACGTGGT ACTTAGTTAG GGTGGATGAA 
AAGCACAGAA GAACTTACGC TCGGCGTCCC ACTCCAAATG ATGACACTTT GGATGAAGGA 
GTAGGACTTG TGCATTCAAA CATAGCAACT GAGCATATTC CAAGCCCTGC CAAGAAAGTA 
CCAAGACTCC CTGCCACAGC AGCAGAACCT GAAGCAGCTG TCATTAGTAA TGGGGAACAT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HMGCS1 ( NM_002130.7 ) cDNA ORF clone, Homo sapiens(human) -> NP_002121.4 Homo sapiens 3-hydroxy-3-methylglutaryl-CoA synthase 1 (HMGCS1), transcript variant 2, mRNA.

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CloneID	OHu01309
Clone ID Related Accession (Same CDS sequence)	NM_001324220.1 , NM_001330663.2 , NM_002130.7 , NM_001098272.3 , NM_001330663.1 , NM_001324219.2 , NM_001364188.2 , NM_001324219.1 , NM_001364188.1 , NM_001098272.2 , NM_002130.8 , NM_001324220.2
Accession Version	NM_002130.7	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1563bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Homo sapiens(human)
Product	hydroxymethylglutaryl-CoA synthase, cytoplasmic isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC114947.2. On May 17, 2012 this sequence version replaced NM_002130.6. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.158150.1, SRR1803613.257271.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03267761 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCTGGAT CACTTCCTTT GAATGCAGAA GCTTGCTGGC CAAAAGATGT GGGAATTGTT 
GCCCTTGAGA TCTATTTTCC TTCTCAATAT GTTGATCAAG CAGAGTTGGA AAAATATGAT 
GGTGTAGATG CTGGAAAGTA TACCATTGGC TTGGGCCAGG CCAAGATGGG CTTCTGCACA 
GATAGAGAAG ATATTAACTC TCTTTGCATG ACTGTGGTTC AGAATCTTAT GGAGAGAAAT 
AACCTTTCCT ATGATTGCAT TGGGCGGCTG GAAGTTGGAA CAGAGACAAT CATCGACAAA 
TCAAAGTCTG TGAAGACTAA TTTGATGCAG CTGTTTGAAG AGTCTGGGAA TACAGATATA 
GAAGGAATCG ACACAACTAA TGCATGCTAT GGAGGCACAG CTGCTGTCTT CAATGCTGTT 
AACTGGATTG AGTCCAGCTC TTGGGATGGA CGGTATGCCC TGGTAGTTGC AGGAGATATT 
GCTGTATATG CCACAGGAAA TGCTAGACCT ACAGGTGGAG TTGGAGCAGT AGCTCTGCTA 
ATTGGGCCAA ATGCTCCTTT AATTTTTGAA CGAGGGCTTC GTGGGACACA TATGCAACAT 
GCCTATGATT TTTACAAGCC TGATATGCTA TCTGAATATC CTATAGTAGA TGGAAAACTC 
TCCATACAGT GCTACCTCAG TGCATTAGAC CGCTGCTATT CTGTCTACTG CAAAAAGATC 
CATGCCCAGT GGCAGAAAGA GGGAAATGAT AAAGATTTTA CCTTGAATGA TTTTGGCTTC 
ATGATCTTTC ACTCACCATA TTGTAAACTG GTTCAGAAAT CTCTAGCTCG GATGTTGCTG 
AATGACTTCC TTAATGACCA GAATAGAGAT AAAAATAGTA TCTATAGTGG CCTGGAAGCC 
TTTGGGGATG TTAAATTAGA AGACACCTAC TTTGATAGAG ATGTGGAGAA GGCATTTATG 
AAGGCTAGCT CTGAACTCTT CAGTCAGAAA ACAAAGGCAT CTTTACTTGT ATCAAATCAA 
AATGGAAATA TGTACACATC TTCAGTATAT GGTTCCCTTG CATCTGTTCT AGCACAGTAC 
TCACCTCAGC AATTAGCAGG GAAGAGAATT GGAGTGTTTT CTTATGGTTC TGGTTTGGCT 
GCCACTCTGT ACTCTCTTAA AGTCACACAA GATGCTACAC CGGGGTCTGC TCTTGATAAA 
ATAACAGCAA GTTTATGTGA TCTTAAATCA AGGCTTGATT CAAGAACTGG TGTGGCACCA 
GATGTCTTCG CTGAAAACAT GAAGCTCAGA GAGGACACCC ATCATTTGGT CAACTATATT 
CCCCAGGGTT CAATAGATTC ACTCTTTGAA GGAACGTGGT ACTTAGTTAG GGTGGATGAA 
AAGCACAGAA GAACTTACGC TCGGCGTCCC ACTCCAAATG ATGACACTTT GGATGAAGGA 
GTAGGACTTG TGCATTCAAA CATAGCAACT GAGCATATTC CAAGCCCTGC CAAGAAAGTA 
CCAAGACTCC CTGCCACAGC AGCAGAACCT GAAGCAGCTG TCATTAGTAA TGGGGAACAT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002121.4
CDS	168..1730
Translation	MPGSLPLNAEACWPKDVGIVALEIYFPSQYVDQAELEKYDGVDAGKYTIGLGQAKMGFCTDREDINSLCMTVVQNLMERNNLSYDCIGRLEVGTETIIDKSKSVKTNLMQLFEESGNTDIEGIDTTNACYGGTAAVFNAVNWIESSSWDGRYALVVAGDIAVYATGNARPTGGVGAVALLIGPNAPLIFERGLRGTHMQHAYDFYKPDMLSEYPIVDGKLSIQCYLSALDRCYSVYCKKIHAQWQKEGNDKDFTLNDFGFMIFHSPYCKLVQKSLARMLLNDFLNDQNRDKNSIYSGLEAFGDVKLEDTYFDRDVEKAFMKASSELFSQKTKASLLVSNQNGNMYTSSVYGSLASVLAQYSPQQLAGKRIGVFSYGSGLAATLYSLKVTQDATPGSALDKITASLCDLKSRLDSRTGVAPDVFAENMKLREDTHHLVNYIPQGSIDSLFEGTWYLVRVDEKHRRTYARRPTPNDDTLDEGVGLVHSNIATEHIPSPAKKVPRLPATAAEPEAAVISNGEH

Target ORF information:

RefSeq Version	NM_002130.7
Organism	Homo sapiens(human)
Definition	Homo sapiens 3-hydroxy-3-methylglutaryl-CoA synthase 1 (HMGCS1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002130.7

ORF Insert Sequence:

ATGCCTGGAT CACTTCCTTT GAATGCAGAA GCTTGCTGGC CAAAAGATGT GGGAATTGTT 
GCCCTTGAGA TCTATTTTCC TTCTCAATAT GTTGATCAAG CAGAGTTGGA AAAATATGAT 
GGTGTAGATG CTGGAAAGTA TACCATTGGC TTGGGCCAGG CCAAGATGGG CTTCTGCACA 
GATAGAGAAG ATATTAACTC TCTTTGCATG ACTGTGGTTC AGAATCTTAT GGAGAGAAAT 
AACCTTTCCT ATGATTGCAT TGGGCGGCTG GAAGTTGGAA CAGAGACAAT CATCGACAAA 
TCAAAGTCTG TGAAGACTAA TTTGATGCAG CTGTTTGAAG AGTCTGGGAA TACAGATATA 
GAAGGAATCG ACACAACTAA TGCATGCTAT GGAGGCACAG CTGCTGTCTT CAATGCTGTT 
AACTGGATTG AGTCCAGCTC TTGGGATGGA CGGTATGCCC TGGTAGTTGC AGGAGATATT 
GCTGTATATG CCACAGGAAA TGCTAGACCT ACAGGTGGAG TTGGAGCAGT AGCTCTGCTA 
ATTGGGCCAA ATGCTCCTTT AATTTTTGAA CGAGGGCTTC GTGGGACACA TATGCAACAT 
GCCTATGATT TTTACAAGCC TGATATGCTA TCTGAATATC CTATAGTAGA TGGAAAACTC 
TCCATACAGT GCTACCTCAG TGCATTAGAC CGCTGCTATT CTGTCTACTG CAAAAAGATC 
CATGCCCAGT GGCAGAAAGA GGGAAATGAT AAAGATTTTA CCTTGAATGA TTTTGGCTTC 
ATGATCTTTC ACTCACCATA TTGTAAACTG GTTCAGAAAT CTCTAGCTCG GATGTTGCTG 
AATGACTTCC TTAATGACCA GAATAGAGAT AAAAATAGTA TCTATAGTGG CCTGGAAGCC 
TTTGGGGATG TTAAATTAGA AGACACCTAC TTTGATAGAG ATGTGGAGAA GGCATTTATG 
AAGGCTAGCT CTGAACTCTT CAGTCAGAAA ACAAAGGCAT CTTTACTTGT ATCAAATCAA 
AATGGAAATA TGTACACATC TTCAGTATAT GGTTCCCTTG CATCTGTTCT AGCACAGTAC 
TCACCTCAGC AATTAGCAGG GAAGAGAATT GGAGTGTTTT CTTATGGTTC TGGTTTGGCT 
GCCACTCTGT ACTCTCTTAA AGTCACACAA GATGCTACAC CGGGGTCTGC TCTTGATAAA 
ATAACAGCAA GTTTATGTGA TCTTAAATCA AGGCTTGATT CAAGAACTGG TGTGGCACCA 
GATGTCTTCG CTGAAAACAT GAAGCTCAGA GAGGACACCC ATCATTTGGT CAACTATATT 
CCCCAGGGTT CAATAGATTC ACTCTTTGAA GGAACGTGGT ACTTAGTTAG GGTGGATGAA 
AAGCACAGAA GAACTTACGC TCGGCGTCCC ACTCCAAATG ATGACACTTT GGATGAAGGA 
GTAGGACTTG TGCATTCAAA CATAGCAACT GAGCATATTC CAAGCCCTGC CAAGAAAGTA 
CCAAGACTCC CTGCCACAGC AGCAGAACCT GAAGCAGCTG TCATTAGTAA TGGGGAACAT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HMGCS1 ( NM_001098272.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001091742.1 Homo sapiens 3-hydroxy-3-methylglutaryl-CoA synthase 1 (HMGCS1), transcript variant 1, mRNA.

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CloneID	OHu01309
Clone ID Related Accession (Same CDS sequence)	NM_001324220.1 , NM_001330663.2 , NM_002130.7 , NM_001098272.3 , NM_001330663.1 , NM_001324219.2 , NM_001364188.2 , NM_001324219.1 , NM_001364188.1 , NM_001098272.2 , NM_002130.8 , NM_001324220.2
Accession Version	NM_001098272.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1563bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-25
Organism	Homo sapiens(human)
Product	hydroxymethylglutaryl-CoA synthase, cytoplasmic isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK315593.1 and AC114947.2. On Nov 22, 2018 this sequence version replaced NM_001098272.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.216745.1, SRR1803615.11048.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000325110.11/ ENSP00000322706.6 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCCTGGAT CACTTCCTTT GAATGCAGAA GCTTGCTGGC CAAAAGATGT GGGAATTGTT 
GCCCTTGAGA TCTATTTTCC TTCTCAATAT GTTGATCAAG CAGAGTTGGA AAAATATGAT 
GGTGTAGATG CTGGAAAGTA TACCATTGGC TTGGGCCAGG CCAAGATGGG CTTCTGCACA 
GATAGAGAAG ATATTAACTC TCTTTGCATG ACTGTGGTTC AGAATCTTAT GGAGAGAAAT 
AACCTTTCCT ATGATTGCAT TGGGCGGCTG GAAGTTGGAA CAGAGACAAT CATCGACAAA 
TCAAAGTCTG TGAAGACTAA TTTGATGCAG CTGTTTGAAG AGTCTGGGAA TACAGATATA 
GAAGGAATCG ACACAACTAA TGCATGCTAT GGAGGCACAG CTGCTGTCTT CAATGCTGTT 
AACTGGATTG AGTCCAGCTC TTGGGATGGA CGGTATGCCC TGGTAGTTGC AGGAGATATT 
GCTGTATATG CCACAGGAAA TGCTAGACCT ACAGGTGGAG TTGGAGCAGT AGCTCTGCTA 
ATTGGGCCAA ATGCTCCTTT AATTTTTGAA CGAGGGCTTC GTGGGACACA TATGCAACAT 
GCCTATGATT TTTACAAGCC TGATATGCTA TCTGAATATC CTATAGTAGA TGGAAAACTC 
TCCATACAGT GCTACCTCAG TGCATTAGAC CGCTGCTATT CTGTCTACTG CAAAAAGATC 
CATGCCCAGT GGCAGAAAGA GGGAAATGAT AAAGATTTTA CCTTGAATGA TTTTGGCTTC 
ATGATCTTTC ACTCACCATA TTGTAAACTG GTTCAGAAAT CTCTAGCTCG GATGTTGCTG 
AATGACTTCC TTAATGACCA GAATAGAGAT AAAAATAGTA TCTATAGTGG CCTGGAAGCC 
TTTGGGGATG TTAAATTAGA AGACACCTAC TTTGATAGAG ATGTGGAGAA GGCATTTATG 
AAGGCTAGCT CTGAACTCTT CAGTCAGAAA ACAAAGGCAT CTTTACTTGT ATCAAATCAA 
AATGGAAATA TGTACACATC TTCAGTATAT GGTTCCCTTG CATCTGTTCT AGCACAGTAC 
TCACCTCAGC AATTAGCAGG GAAGAGAATT GGAGTGTTTT CTTATGGTTC TGGTTTGGCT 
GCCACTCTGT ACTCTCTTAA AGTCACACAA GATGCTACAC CGGGGTCTGC TCTTGATAAA 
ATAACAGCAA GTTTATGTGA TCTTAAATCA AGGCTTGATT CAAGAACTGG TGTGGCACCA 
GATGTCTTCG CTGAAAACAT GAAGCTCAGA GAGGACACCC ATCATTTGGT CAACTATATT 
CCCCAGGGTT CAATAGATTC ACTCTTTGAA GGAACGTGGT ACTTAGTTAG GGTGGATGAA 
AAGCACAGAA GAACTTACGC TCGGCGTCCC ACTCCAAATG ATGACACTTT GGATGAAGGA 
GTAGGACTTG TGCATTCAAA CATAGCAACT GAGCATATTC CAAGCCCTGC CAAGAAAGTA 
CCAAGACTCC CTGCCACAGC AGCAGAACCT GAAGCAGCTG TCATTAGTAA TGGGGAACAT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001091742.1
CDS	127..1689
Translation	MPGSLPLNAEACWPKDVGIVALEIYFPSQYVDQAELEKYDGVDAGKYTIGLGQAKMGFCTDREDINSLCMTVVQNLMERNNLSYDCIGRLEVGTETIIDKSKSVKTNLMQLFEESGNTDIEGIDTTNACYGGTAAVFNAVNWIESSSWDGRYALVVAGDIAVYATGNARPTGGVGAVALLIGPNAPLIFERGLRGTHMQHAYDFYKPDMLSEYPIVDGKLSIQCYLSALDRCYSVYCKKIHAQWQKEGNDKDFTLNDFGFMIFHSPYCKLVQKSLARMLLNDFLNDQNRDKNSIYSGLEAFGDVKLEDTYFDRDVEKAFMKASSELFSQKTKASLLVSNQNGNMYTSSVYGSLASVLAQYSPQQLAGKRIGVFSYGSGLAATLYSLKVTQDATPGSALDKITASLCDLKSRLDSRTGVAPDVFAENMKLREDTHHLVNYIPQGSIDSLFEGTWYLVRVDEKHRRTYARRPTPNDDTLDEGVGLVHSNIATEHIPSPAKKVPRLPATAAEPEAAVISNGEH

Target ORF information:

RefSeq Version	NM_001098272.3
Organism	Homo sapiens(human)
Definition	Homo sapiens 3-hydroxy-3-methylglutaryl-CoA synthase 1 (HMGCS1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001098272.3

ORF Insert Sequence:

ATGCCTGGAT CACTTCCTTT GAATGCAGAA GCTTGCTGGC CAAAAGATGT GGGAATTGTT 
GCCCTTGAGA TCTATTTTCC TTCTCAATAT GTTGATCAAG CAGAGTTGGA AAAATATGAT 
GGTGTAGATG CTGGAAAGTA TACCATTGGC TTGGGCCAGG CCAAGATGGG CTTCTGCACA 
GATAGAGAAG ATATTAACTC TCTTTGCATG ACTGTGGTTC AGAATCTTAT GGAGAGAAAT 
AACCTTTCCT ATGATTGCAT TGGGCGGCTG GAAGTTGGAA CAGAGACAAT CATCGACAAA 
TCAAAGTCTG TGAAGACTAA TTTGATGCAG CTGTTTGAAG AGTCTGGGAA TACAGATATA 
GAAGGAATCG ACACAACTAA TGCATGCTAT GGAGGCACAG CTGCTGTCTT CAATGCTGTT 
AACTGGATTG AGTCCAGCTC TTGGGATGGA CGGTATGCCC TGGTAGTTGC AGGAGATATT 
GCTGTATATG CCACAGGAAA TGCTAGACCT ACAGGTGGAG TTGGAGCAGT AGCTCTGCTA 
ATTGGGCCAA ATGCTCCTTT AATTTTTGAA CGAGGGCTTC GTGGGACACA TATGCAACAT 
GCCTATGATT TTTACAAGCC TGATATGCTA TCTGAATATC CTATAGTAGA TGGAAAACTC 
TCCATACAGT GCTACCTCAG TGCATTAGAC CGCTGCTATT CTGTCTACTG CAAAAAGATC 
CATGCCCAGT GGCAGAAAGA GGGAAATGAT AAAGATTTTA CCTTGAATGA TTTTGGCTTC 
ATGATCTTTC ACTCACCATA TTGTAAACTG GTTCAGAAAT CTCTAGCTCG GATGTTGCTG 
AATGACTTCC TTAATGACCA GAATAGAGAT AAAAATAGTA TCTATAGTGG CCTGGAAGCC 
TTTGGGGATG TTAAATTAGA AGACACCTAC TTTGATAGAG ATGTGGAGAA GGCATTTATG 
AAGGCTAGCT CTGAACTCTT CAGTCAGAAA ACAAAGGCAT CTTTACTTGT ATCAAATCAA 
AATGGAAATA TGTACACATC TTCAGTATAT GGTTCCCTTG CATCTGTTCT AGCACAGTAC 
TCACCTCAGC AATTAGCAGG GAAGAGAATT GGAGTGTTTT CTTATGGTTC TGGTTTGGCT 
GCCACTCTGT ACTCTCTTAA AGTCACACAA GATGCTACAC CGGGGTCTGC TCTTGATAAA 
ATAACAGCAA GTTTATGTGA TCTTAAATCA AGGCTTGATT CAAGAACTGG TGTGGCACCA 
GATGTCTTCG CTGAAAACAT GAAGCTCAGA GAGGACACCC ATCATTTGGT CAACTATATT 
CCCCAGGGTT CAATAGATTC ACTCTTTGAA GGAACGTGGT ACTTAGTTAG GGTGGATGAA 
AAGCACAGAA GAACTTACGC TCGGCGTCCC ACTCCAAATG ATGACACTTT GGATGAAGGA 
GTAGGACTTG TGCATTCAAA CATAGCAACT GAGCATATTC CAAGCCCTGC CAAGAAAGTA 
CCAAGACTCC CTGCCACAGC AGCAGAACCT GAAGCAGCTG TCATTAGTAA TGGGGAACAT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HMGCS1 ( NM_001330663.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001317592.1 Homo sapiens 3-hydroxy-3-methylglutaryl-CoA synthase 1 (HMGCS1), transcript variant 8, mRNA.

Price & Availability↑

CloneID	OHu01309
Clone ID Related Accession (Same CDS sequence)	NM_001324220.1 , NM_001330663.2 , NM_002130.7 , NM_001098272.3 , NM_001330663.1 , NM_001324219.2 , NM_001364188.2 , NM_001324219.1 , NM_001364188.1 , NM_001098272.2 , NM_002130.8 , NM_001324220.2
Accession Version	NM_001330663.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1563bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-25
Organism	Homo sapiens(human)
Product	hydroxymethylglutaryl-CoA synthase, cytoplasmic isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC114947.2. On Sep 1, 2016 this sequence version replaced XM_017009412.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.96054.1, SRR1660807.126299.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCTGGAT CACTTCCTTT GAATGCAGAA GCTTGCTGGC CAAAAGATGT GGGAATTGTT 
GCCCTTGAGA TCTATTTTCC TTCTCAATAT GTTGATCAAG CAGAGTTGGA AAAATATGAT 
GGTGTAGATG CTGGAAAGTA TACCATTGGC TTGGGCCAGG CCAAGATGGG CTTCTGCACA 
GATAGAGAAG ATATTAACTC TCTTTGCATG ACTGTGGTTC AGAATCTTAT GGAGAGAAAT 
AACCTTTCCT ATGATTGCAT TGGGCGGCTG GAAGTTGGAA CAGAGACAAT CATCGACAAA 
TCAAAGTCTG TGAAGACTAA TTTGATGCAG CTGTTTGAAG AGTCTGGGAA TACAGATATA 
GAAGGAATCG ACACAACTAA TGCATGCTAT GGAGGCACAG CTGCTGTCTT CAATGCTGTT 
AACTGGATTG AGTCCAGCTC TTGGGATGGA CGGTATGCCC TGGTAGTTGC AGGAGATATT 
GCTGTATATG CCACAGGAAA TGCTAGACCT ACAGGTGGAG TTGGAGCAGT AGCTCTGCTA 
ATTGGGCCAA ATGCTCCTTT AATTTTTGAA CGAGGGCTTC GTGGGACACA TATGCAACAT 
GCCTATGATT TTTACAAGCC TGATATGCTA TCTGAATATC CTATAGTAGA TGGAAAACTC 
TCCATACAGT GCTACCTCAG TGCATTAGAC CGCTGCTATT CTGTCTACTG CAAAAAGATC 
CATGCCCAGT GGCAGAAAGA GGGAAATGAT AAAGATTTTA CCTTGAATGA TTTTGGCTTC 
ATGATCTTTC ACTCACCATA TTGTAAACTG GTTCAGAAAT CTCTAGCTCG GATGTTGCTG 
AATGACTTCC TTAATGACCA GAATAGAGAT AAAAATAGTA TCTATAGTGG CCTGGAAGCC 
TTTGGGGATG TTAAATTAGA AGACACCTAC TTTGATAGAG ATGTGGAGAA GGCATTTATG 
AAGGCTAGCT CTGAACTCTT CAGTCAGAAA ACAAAGGCAT CTTTACTTGT ATCAAATCAA 
AATGGAAATA TGTACACATC TTCAGTATAT GGTTCCCTTG CATCTGTTCT AGCACAGTAC 
TCACCTCAGC AATTAGCAGG GAAGAGAATT GGAGTGTTTT CTTATGGTTC TGGTTTGGCT 
GCCACTCTGT ACTCTCTTAA AGTCACACAA GATGCTACAC CGGGGTCTGC TCTTGATAAA 
ATAACAGCAA GTTTATGTGA TCTTAAATCA AGGCTTGATT CAAGAACTGG TGTGGCACCA 
GATGTCTTCG CTGAAAACAT GAAGCTCAGA GAGGACACCC ATCATTTGGT CAACTATATT 
CCCCAGGGTT CAATAGATTC ACTCTTTGAA GGAACGTGGT ACTTAGTTAG GGTGGATGAA 
AAGCACAGAA GAACTTACGC TCGGCGTCCC ACTCCAAATG ATGACACTTT GGATGAAGGA 
GTAGGACTTG TGCATTCAAA CATAGCAACT GAGCATATTC CAAGCCCTGC CAAGAAAGTA 
CCAAGACTCC CTGCCACAGC AGCAGAACCT GAAGCAGCTG TCATTAGTAA TGGGGAACAT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001317592.1
CDS	346..1908
Translation	MPGSLPLNAEACWPKDVGIVALEIYFPSQYVDQAELEKYDGVDAGKYTIGLGQAKMGFCTDREDINSLCMTVVQNLMERNNLSYDCIGRLEVGTETIIDKSKSVKTNLMQLFEESGNTDIEGIDTTNACYGGTAAVFNAVNWIESSSWDGRYALVVAGDIAVYATGNARPTGGVGAVALLIGPNAPLIFERGLRGTHMQHAYDFYKPDMLSEYPIVDGKLSIQCYLSALDRCYSVYCKKIHAQWQKEGNDKDFTLNDFGFMIFHSPYCKLVQKSLARMLLNDFLNDQNRDKNSIYSGLEAFGDVKLEDTYFDRDVEKAFMKASSELFSQKTKASLLVSNQNGNMYTSSVYGSLASVLAQYSPQQLAGKRIGVFSYGSGLAATLYSLKVTQDATPGSALDKITASLCDLKSRLDSRTGVAPDVFAENMKLREDTHHLVNYIPQGSIDSLFEGTWYLVRVDEKHRRTYARRPTPNDDTLDEGVGLVHSNIATEHIPSPAKKVPRLPATAAEPEAAVISNGEH

Target ORF information:

RefSeq Version	NM_001330663.1
Organism	Homo sapiens(human)
Definition	Homo sapiens 3-hydroxy-3-methylglutaryl-CoA synthase 1 (HMGCS1), transcript variant 8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001330663.1

ORF Insert Sequence:

ATGCCTGGAT CACTTCCTTT GAATGCAGAA GCTTGCTGGC CAAAAGATGT GGGAATTGTT 
GCCCTTGAGA TCTATTTTCC TTCTCAATAT GTTGATCAAG CAGAGTTGGA AAAATATGAT 
GGTGTAGATG CTGGAAAGTA TACCATTGGC TTGGGCCAGG CCAAGATGGG CTTCTGCACA 
GATAGAGAAG ATATTAACTC TCTTTGCATG ACTGTGGTTC AGAATCTTAT GGAGAGAAAT 
AACCTTTCCT ATGATTGCAT TGGGCGGCTG GAAGTTGGAA CAGAGACAAT CATCGACAAA 
TCAAAGTCTG TGAAGACTAA TTTGATGCAG CTGTTTGAAG AGTCTGGGAA TACAGATATA 
GAAGGAATCG ACACAACTAA TGCATGCTAT GGAGGCACAG CTGCTGTCTT CAATGCTGTT 
AACTGGATTG AGTCCAGCTC TTGGGATGGA CGGTATGCCC TGGTAGTTGC AGGAGATATT 
GCTGTATATG CCACAGGAAA TGCTAGACCT ACAGGTGGAG TTGGAGCAGT AGCTCTGCTA 
ATTGGGCCAA ATGCTCCTTT AATTTTTGAA CGAGGGCTTC GTGGGACACA TATGCAACAT 
GCCTATGATT TTTACAAGCC TGATATGCTA TCTGAATATC CTATAGTAGA TGGAAAACTC 
TCCATACAGT GCTACCTCAG TGCATTAGAC CGCTGCTATT CTGTCTACTG CAAAAAGATC 
CATGCCCAGT GGCAGAAAGA GGGAAATGAT AAAGATTTTA CCTTGAATGA TTTTGGCTTC 
ATGATCTTTC ACTCACCATA TTGTAAACTG GTTCAGAAAT CTCTAGCTCG GATGTTGCTG 
AATGACTTCC TTAATGACCA GAATAGAGAT AAAAATAGTA TCTATAGTGG CCTGGAAGCC 
TTTGGGGATG TTAAATTAGA AGACACCTAC TTTGATAGAG ATGTGGAGAA GGCATTTATG 
AAGGCTAGCT CTGAACTCTT CAGTCAGAAA ACAAAGGCAT CTTTACTTGT ATCAAATCAA 
AATGGAAATA TGTACACATC TTCAGTATAT GGTTCCCTTG CATCTGTTCT AGCACAGTAC 
TCACCTCAGC AATTAGCAGG GAAGAGAATT GGAGTGTTTT CTTATGGTTC TGGTTTGGCT 
GCCACTCTGT ACTCTCTTAA AGTCACACAA GATGCTACAC CGGGGTCTGC TCTTGATAAA 
ATAACAGCAA GTTTATGTGA TCTTAAATCA AGGCTTGATT CAAGAACTGG TGTGGCACCA 
GATGTCTTCG CTGAAAACAT GAAGCTCAGA GAGGACACCC ATCATTTGGT CAACTATATT 
CCCCAGGGTT CAATAGATTC ACTCTTTGAA GGAACGTGGT ACTTAGTTAG GGTGGATGAA 
AAGCACAGAA GAACTTACGC TCGGCGTCCC ACTCCAAATG ATGACACTTT GGATGAAGGA 
GTAGGACTTG TGCATTCAAA CATAGCAACT GAGCATATTC CAAGCCCTGC CAAGAAAGTA 
CCAAGACTCC CTGCCACAGC AGCAGAACCT GAAGCAGCTG TCATTAGTAA TGGGGAACAT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HMGCS1 ( NM_001324219.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001311148.1 Homo sapiens 3-hydroxy-3-methylglutaryl-CoA synthase 1 (HMGCS1), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu01309
Clone ID Related Accession (Same CDS sequence)	NM_001324220.1 , NM_001330663.2 , NM_002130.7 , NM_001098272.3 , NM_001330663.1 , NM_001324219.2 , NM_001364188.2 , NM_001324219.1 , NM_001364188.1 , NM_001098272.2 , NM_002130.8 , NM_001324220.2
Accession Version	NM_001324219.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1563bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-27
Organism	Homo sapiens(human)
Product	hydroxymethylglutaryl-CoA synthase, cytoplasmic isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DA801434.1, AK315593.1 and AC114947.2. On May 31, 2019 this sequence version replaced NM_001324219.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.2816.1, SRR1803616.112840.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03267755, SAMN03267761 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCTGGAT CACTTCCTTT GAATGCAGAA GCTTGCTGGC CAAAAGATGT GGGAATTGTT 
GCCCTTGAGA TCTATTTTCC TTCTCAATAT GTTGATCAAG CAGAGTTGGA AAAATATGAT 
GGTGTAGATG CTGGAAAGTA TACCATTGGC TTGGGCCAGG CCAAGATGGG CTTCTGCACA 
GATAGAGAAG ATATTAACTC TCTTTGCATG ACTGTGGTTC AGAATCTTAT GGAGAGAAAT 
AACCTTTCCT ATGATTGCAT TGGGCGGCTG GAAGTTGGAA CAGAGACAAT CATCGACAAA 
TCAAAGTCTG TGAAGACTAA TTTGATGCAG CTGTTTGAAG AGTCTGGGAA TACAGATATA 
GAAGGAATCG ACACAACTAA TGCATGCTAT GGAGGCACAG CTGCTGTCTT CAATGCTGTT 
AACTGGATTG AGTCCAGCTC TTGGGATGGA CGGTATGCCC TGGTAGTTGC AGGAGATATT 
GCTGTATATG CCACAGGAAA TGCTAGACCT ACAGGTGGAG TTGGAGCAGT AGCTCTGCTA 
ATTGGGCCAA ATGCTCCTTT AATTTTTGAA CGAGGGCTTC GTGGGACACA TATGCAACAT 
GCCTATGATT TTTACAAGCC TGATATGCTA TCTGAATATC CTATAGTAGA TGGAAAACTC 
TCCATACAGT GCTACCTCAG TGCATTAGAC CGCTGCTATT CTGTCTACTG CAAAAAGATC 
CATGCCCAGT GGCAGAAAGA GGGAAATGAT AAAGATTTTA CCTTGAATGA TTTTGGCTTC 
ATGATCTTTC ACTCACCATA TTGTAAACTG GTTCAGAAAT CTCTAGCTCG GATGTTGCTG 
AATGACTTCC TTAATGACCA GAATAGAGAT AAAAATAGTA TCTATAGTGG CCTGGAAGCC 
TTTGGGGATG TTAAATTAGA AGACACCTAC TTTGATAGAG ATGTGGAGAA GGCATTTATG 
AAGGCTAGCT CTGAACTCTT CAGTCAGAAA ACAAAGGCAT CTTTACTTGT ATCAAATCAA 
AATGGAAATA TGTACACATC TTCAGTATAT GGTTCCCTTG CATCTGTTCT AGCACAGTAC 
TCACCTCAGC AATTAGCAGG GAAGAGAATT GGAGTGTTTT CTTATGGTTC TGGTTTGGCT 
GCCACTCTGT ACTCTCTTAA AGTCACACAA GATGCTACAC CGGGGTCTGC TCTTGATAAA 
ATAACAGCAA GTTTATGTGA TCTTAAATCA AGGCTTGATT CAAGAACTGG TGTGGCACCA 
GATGTCTTCG CTGAAAACAT GAAGCTCAGA GAGGACACCC ATCATTTGGT CAACTATATT 
CCCCAGGGTT CAATAGATTC ACTCTTTGAA GGAACGTGGT ACTTAGTTAG GGTGGATGAA 
AAGCACAGAA GAACTTACGC TCGGCGTCCC ACTCCAAATG ATGACACTTT GGATGAAGGA 
GTAGGACTTG TGCATTCAAA CATAGCAACT GAGCATATTC CAAGCCCTGC CAAGAAAGTA 
CCAAGACTCC CTGCCACAGC AGCAGAACCT GAAGCAGCTG TCATTAGTAA TGGGGAACAT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001311148.1
CDS	171..1733
Translation	MPGSLPLNAEACWPKDVGIVALEIYFPSQYVDQAELEKYDGVDAGKYTIGLGQAKMGFCTDREDINSLCMTVVQNLMERNNLSYDCIGRLEVGTETIIDKSKSVKTNLMQLFEESGNTDIEGIDTTNACYGGTAAVFNAVNWIESSSWDGRYALVVAGDIAVYATGNARPTGGVGAVALLIGPNAPLIFERGLRGTHMQHAYDFYKPDMLSEYPIVDGKLSIQCYLSALDRCYSVYCKKIHAQWQKEGNDKDFTLNDFGFMIFHSPYCKLVQKSLARMLLNDFLNDQNRDKNSIYSGLEAFGDVKLEDTYFDRDVEKAFMKASSELFSQKTKASLLVSNQNGNMYTSSVYGSLASVLAQYSPQQLAGKRIGVFSYGSGLAATLYSLKVTQDATPGSALDKITASLCDLKSRLDSRTGVAPDVFAENMKLREDTHHLVNYIPQGSIDSLFEGTWYLVRVDEKHRRTYARRPTPNDDTLDEGVGLVHSNIATEHIPSPAKKVPRLPATAAEPEAAVISNGEH

Target ORF information:

RefSeq Version	NM_001324219.2
Organism	Homo sapiens(human)
Definition	Homo sapiens 3-hydroxy-3-methylglutaryl-CoA synthase 1 (HMGCS1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001324219.2

ORF Insert Sequence:

ATGCCTGGAT CACTTCCTTT GAATGCAGAA GCTTGCTGGC CAAAAGATGT GGGAATTGTT 
GCCCTTGAGA TCTATTTTCC TTCTCAATAT GTTGATCAAG CAGAGTTGGA AAAATATGAT 
GGTGTAGATG CTGGAAAGTA TACCATTGGC TTGGGCCAGG CCAAGATGGG CTTCTGCACA 
GATAGAGAAG ATATTAACTC TCTTTGCATG ACTGTGGTTC AGAATCTTAT GGAGAGAAAT 
AACCTTTCCT ATGATTGCAT TGGGCGGCTG GAAGTTGGAA CAGAGACAAT CATCGACAAA 
TCAAAGTCTG TGAAGACTAA TTTGATGCAG CTGTTTGAAG AGTCTGGGAA TACAGATATA 
GAAGGAATCG ACACAACTAA TGCATGCTAT GGAGGCACAG CTGCTGTCTT CAATGCTGTT 
AACTGGATTG AGTCCAGCTC TTGGGATGGA CGGTATGCCC TGGTAGTTGC AGGAGATATT 
GCTGTATATG CCACAGGAAA TGCTAGACCT ACAGGTGGAG TTGGAGCAGT AGCTCTGCTA 
ATTGGGCCAA ATGCTCCTTT AATTTTTGAA CGAGGGCTTC GTGGGACACA TATGCAACAT 
GCCTATGATT TTTACAAGCC TGATATGCTA TCTGAATATC CTATAGTAGA TGGAAAACTC 
TCCATACAGT GCTACCTCAG TGCATTAGAC CGCTGCTATT CTGTCTACTG CAAAAAGATC 
CATGCCCAGT GGCAGAAAGA GGGAAATGAT AAAGATTTTA CCTTGAATGA TTTTGGCTTC 
ATGATCTTTC ACTCACCATA TTGTAAACTG GTTCAGAAAT CTCTAGCTCG GATGTTGCTG 
AATGACTTCC TTAATGACCA GAATAGAGAT AAAAATAGTA TCTATAGTGG CCTGGAAGCC 
TTTGGGGATG TTAAATTAGA AGACACCTAC TTTGATAGAG ATGTGGAGAA GGCATTTATG 
AAGGCTAGCT CTGAACTCTT CAGTCAGAAA ACAAAGGCAT CTTTACTTGT ATCAAATCAA 
AATGGAAATA TGTACACATC TTCAGTATAT GGTTCCCTTG CATCTGTTCT AGCACAGTAC 
TCACCTCAGC AATTAGCAGG GAAGAGAATT GGAGTGTTTT CTTATGGTTC TGGTTTGGCT 
GCCACTCTGT ACTCTCTTAA AGTCACACAA GATGCTACAC CGGGGTCTGC TCTTGATAAA 
ATAACAGCAA GTTTATGTGA TCTTAAATCA AGGCTTGATT CAAGAACTGG TGTGGCACCA 
GATGTCTTCG CTGAAAACAT GAAGCTCAGA GAGGACACCC ATCATTTGGT CAACTATATT 
CCCCAGGGTT CAATAGATTC ACTCTTTGAA GGAACGTGGT ACTTAGTTAG GGTGGATGAA 
AAGCACAGAA GAACTTACGC TCGGCGTCCC ACTCCAAATG ATGACACTTT GGATGAAGGA 
GTAGGACTTG TGCATTCAAA CATAGCAACT GAGCATATTC CAAGCCCTGC CAAGAAAGTA 
CCAAGACTCC CTGCCACAGC AGCAGAACCT GAAGCAGCTG TCATTAGTAA TGGGGAACAT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HMGCS1 ( NM_001364188.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001351117.1 Homo sapiens 3-hydroxy-3-methylglutaryl-CoA synthase 1 (HMGCS1), transcript variant 9, mRNA.

Price & Availability↑

CloneID	OHu01309
Clone ID Related Accession (Same CDS sequence)	NM_001324220.1 , NM_001330663.2 , NM_002130.7 , NM_001098272.3 , NM_001330663.1 , NM_001324219.2 , NM_001364188.2 , NM_001324219.1 , NM_001364188.1 , NM_001098272.2 , NM_002130.8 , NM_001324220.2
Accession Version	NM_001364188.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1563bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-27
Organism	Homo sapiens(human)
Product	hydroxymethylglutaryl-CoA synthase, cytoplasmic isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC114947.2. On May 31, 2019 this sequence version replaced NM_001364188.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.42317.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

ATGCCTGGAT CACTTCCTTT GAATGCAGAA GCTTGCTGGC CAAAAGATGT GGGAATTGTT 
GCCCTTGAGA TCTATTTTCC TTCTCAATAT GTTGATCAAG CAGAGTTGGA AAAATATGAT 
GGTGTAGATG CTGGAAAGTA TACCATTGGC TTGGGCCAGG CCAAGATGGG CTTCTGCACA 
GATAGAGAAG ATATTAACTC TCTTTGCATG ACTGTGGTTC AGAATCTTAT GGAGAGAAAT 
AACCTTTCCT ATGATTGCAT TGGGCGGCTG GAAGTTGGAA CAGAGACAAT CATCGACAAA 
TCAAAGTCTG TGAAGACTAA TTTGATGCAG CTGTTTGAAG AGTCTGGGAA TACAGATATA 
GAAGGAATCG ACACAACTAA TGCATGCTAT GGAGGCACAG CTGCTGTCTT CAATGCTGTT 
AACTGGATTG AGTCCAGCTC TTGGGATGGA CGGTATGCCC TGGTAGTTGC AGGAGATATT 
GCTGTATATG CCACAGGAAA TGCTAGACCT ACAGGTGGAG TTGGAGCAGT AGCTCTGCTA 
ATTGGGCCAA ATGCTCCTTT AATTTTTGAA CGAGGGCTTC GTGGGACACA TATGCAACAT 
GCCTATGATT TTTACAAGCC TGATATGCTA TCTGAATATC CTATAGTAGA TGGAAAACTC 
TCCATACAGT GCTACCTCAG TGCATTAGAC CGCTGCTATT CTGTCTACTG CAAAAAGATC 
CATGCCCAGT GGCAGAAAGA GGGAAATGAT AAAGATTTTA CCTTGAATGA TTTTGGCTTC 
ATGATCTTTC ACTCACCATA TTGTAAACTG GTTCAGAAAT CTCTAGCTCG GATGTTGCTG 
AATGACTTCC TTAATGACCA GAATAGAGAT AAAAATAGTA TCTATAGTGG CCTGGAAGCC 
TTTGGGGATG TTAAATTAGA AGACACCTAC TTTGATAGAG ATGTGGAGAA GGCATTTATG 
AAGGCTAGCT CTGAACTCTT CAGTCAGAAA ACAAAGGCAT CTTTACTTGT ATCAAATCAA 
AATGGAAATA TGTACACATC TTCAGTATAT GGTTCCCTTG CATCTGTTCT AGCACAGTAC 
TCACCTCAGC AATTAGCAGG GAAGAGAATT GGAGTGTTTT CTTATGGTTC TGGTTTGGCT 
GCCACTCTGT ACTCTCTTAA AGTCACACAA GATGCTACAC CGGGGTCTGC TCTTGATAAA 
ATAACAGCAA GTTTATGTGA TCTTAAATCA AGGCTTGATT CAAGAACTGG TGTGGCACCA 
GATGTCTTCG CTGAAAACAT GAAGCTCAGA GAGGACACCC ATCATTTGGT CAACTATATT 
CCCCAGGGTT CAATAGATTC ACTCTTTGAA GGAACGTGGT ACTTAGTTAG GGTGGATGAA 
AAGCACAGAA GAACTTACGC TCGGCGTCCC ACTCCAAATG ATGACACTTT GGATGAAGGA 
GTAGGACTTG TGCATTCAAA CATAGCAACT GAGCATATTC CAAGCCCTGC CAAGAAAGTA 
CCAAGACTCC CTGCCACAGC AGCAGAACCT GAAGCAGCTG TCATTAGTAA TGGGGAACAT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001351117.1
CDS	250..1812
Translation	MPGSLPLNAEACWPKDVGIVALEIYFPSQYVDQAELEKYDGVDAGKYTIGLGQAKMGFCTDREDINSLCMTVVQNLMERNNLSYDCIGRLEVGTETIIDKSKSVKTNLMQLFEESGNTDIEGIDTTNACYGGTAAVFNAVNWIESSSWDGRYALVVAGDIAVYATGNARPTGGVGAVALLIGPNAPLIFERGLRGTHMQHAYDFYKPDMLSEYPIVDGKLSIQCYLSALDRCYSVYCKKIHAQWQKEGNDKDFTLNDFGFMIFHSPYCKLVQKSLARMLLNDFLNDQNRDKNSIYSGLEAFGDVKLEDTYFDRDVEKAFMKASSELFSQKTKASLLVSNQNGNMYTSSVYGSLASVLAQYSPQQLAGKRIGVFSYGSGLAATLYSLKVTQDATPGSALDKITASLCDLKSRLDSRTGVAPDVFAENMKLREDTHHLVNYIPQGSIDSLFEGTWYLVRVDEKHRRTYARRPTPNDDTLDEGVGLVHSNIATEHIPSPAKKVPRLPATAAEPEAAVISNGEH

Target ORF information:

RefSeq Version	NM_001364188.2
Organism	Homo sapiens(human)
Definition	Homo sapiens 3-hydroxy-3-methylglutaryl-CoA synthase 1 (HMGCS1), transcript variant 9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001364188.2

ORF Insert Sequence:

ATGCCTGGAT CACTTCCTTT GAATGCAGAA GCTTGCTGGC CAAAAGATGT GGGAATTGTT 
GCCCTTGAGA TCTATTTTCC TTCTCAATAT GTTGATCAAG CAGAGTTGGA AAAATATGAT 
GGTGTAGATG CTGGAAAGTA TACCATTGGC TTGGGCCAGG CCAAGATGGG CTTCTGCACA 
GATAGAGAAG ATATTAACTC TCTTTGCATG ACTGTGGTTC AGAATCTTAT GGAGAGAAAT 
AACCTTTCCT ATGATTGCAT TGGGCGGCTG GAAGTTGGAA CAGAGACAAT CATCGACAAA 
TCAAAGTCTG TGAAGACTAA TTTGATGCAG CTGTTTGAAG AGTCTGGGAA TACAGATATA 
GAAGGAATCG ACACAACTAA TGCATGCTAT GGAGGCACAG CTGCTGTCTT CAATGCTGTT 
AACTGGATTG AGTCCAGCTC TTGGGATGGA CGGTATGCCC TGGTAGTTGC AGGAGATATT 
GCTGTATATG CCACAGGAAA TGCTAGACCT ACAGGTGGAG TTGGAGCAGT AGCTCTGCTA 
ATTGGGCCAA ATGCTCCTTT AATTTTTGAA CGAGGGCTTC GTGGGACACA TATGCAACAT 
GCCTATGATT TTTACAAGCC TGATATGCTA TCTGAATATC CTATAGTAGA TGGAAAACTC 
TCCATACAGT GCTACCTCAG TGCATTAGAC CGCTGCTATT CTGTCTACTG CAAAAAGATC 
CATGCCCAGT GGCAGAAAGA GGGAAATGAT AAAGATTTTA CCTTGAATGA TTTTGGCTTC 
ATGATCTTTC ACTCACCATA TTGTAAACTG GTTCAGAAAT CTCTAGCTCG GATGTTGCTG 
AATGACTTCC TTAATGACCA GAATAGAGAT AAAAATAGTA TCTATAGTGG CCTGGAAGCC 
TTTGGGGATG TTAAATTAGA AGACACCTAC TTTGATAGAG ATGTGGAGAA GGCATTTATG 
AAGGCTAGCT CTGAACTCTT CAGTCAGAAA ACAAAGGCAT CTTTACTTGT ATCAAATCAA 
AATGGAAATA TGTACACATC TTCAGTATAT GGTTCCCTTG CATCTGTTCT AGCACAGTAC 
TCACCTCAGC AATTAGCAGG GAAGAGAATT GGAGTGTTTT CTTATGGTTC TGGTTTGGCT 
GCCACTCTGT ACTCTCTTAA AGTCACACAA GATGCTACAC CGGGGTCTGC TCTTGATAAA 
ATAACAGCAA GTTTATGTGA TCTTAAATCA AGGCTTGATT CAAGAACTGG TGTGGCACCA 
GATGTCTTCG CTGAAAACAT GAAGCTCAGA GAGGACACCC ATCATTTGGT CAACTATATT 
CCCCAGGGTT CAATAGATTC ACTCTTTGAA GGAACGTGGT ACTTAGTTAG GGTGGATGAA 
AAGCACAGAA GAACTTACGC TCGGCGTCCC ACTCCAAATG ATGACACTTT GGATGAAGGA 
GTAGGACTTG TGCATTCAAA CATAGCAACT GAGCATATTC CAAGCCCTGC CAAGAAAGTA 
CCAAGACTCC CTGCCACAGC AGCAGAACCT GAAGCAGCTG TCATTAGTAA TGGGGAACAT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HMGCS1 ( NM_001324219.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001311148.1 Homo sapiens 3-hydroxy-3-methylglutaryl-CoA synthase 1 (HMGCS1), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu01309
Clone ID Related Accession (Same CDS sequence)	NM_001324220.1 , NM_001330663.2 , NM_002130.7 , NM_001098272.3 , NM_001330663.1 , NM_001324219.2 , NM_001364188.2 , NM_001324219.1 , NM_001364188.1 , NM_001098272.2 , NM_002130.8 , NM_001324220.2
Accession Version	NM_001324219.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1563bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-25
Organism	Homo sapiens(human)
Product	hydroxymethylglutaryl-CoA synthase, cytoplasmic isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DA801434.1, AK315593.1 and AC114947.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.252369.1, SRR1803616.2816.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03267755, SAMN03267761 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCTGGAT CACTTCCTTT GAATGCAGAA GCTTGCTGGC CAAAAGATGT GGGAATTGTT 
GCCCTTGAGA TCTATTTTCC TTCTCAATAT GTTGATCAAG CAGAGTTGGA AAAATATGAT 
GGTGTAGATG CTGGAAAGTA TACCATTGGC TTGGGCCAGG CCAAGATGGG CTTCTGCACA 
GATAGAGAAG ATATTAACTC TCTTTGCATG ACTGTGGTTC AGAATCTTAT GGAGAGAAAT 
AACCTTTCCT ATGATTGCAT TGGGCGGCTG GAAGTTGGAA CAGAGACAAT CATCGACAAA 
TCAAAGTCTG TGAAGACTAA TTTGATGCAG CTGTTTGAAG AGTCTGGGAA TACAGATATA 
GAAGGAATCG ACACAACTAA TGCATGCTAT GGAGGCACAG CTGCTGTCTT CAATGCTGTT 
AACTGGATTG AGTCCAGCTC TTGGGATGGA CGGTATGCCC TGGTAGTTGC AGGAGATATT 
GCTGTATATG CCACAGGAAA TGCTAGACCT ACAGGTGGAG TTGGAGCAGT AGCTCTGCTA 
ATTGGGCCAA ATGCTCCTTT AATTTTTGAA CGAGGGCTTC GTGGGACACA TATGCAACAT 
GCCTATGATT TTTACAAGCC TGATATGCTA TCTGAATATC CTATAGTAGA TGGAAAACTC 
TCCATACAGT GCTACCTCAG TGCATTAGAC CGCTGCTATT CTGTCTACTG CAAAAAGATC 
CATGCCCAGT GGCAGAAAGA GGGAAATGAT AAAGATTTTA CCTTGAATGA TTTTGGCTTC 
ATGATCTTTC ACTCACCATA TTGTAAACTG GTTCAGAAAT CTCTAGCTCG GATGTTGCTG 
AATGACTTCC TTAATGACCA GAATAGAGAT AAAAATAGTA TCTATAGTGG CCTGGAAGCC 
TTTGGGGATG TTAAATTAGA AGACACCTAC TTTGATAGAG ATGTGGAGAA GGCATTTATG 
AAGGCTAGCT CTGAACTCTT CAGTCAGAAA ACAAAGGCAT CTTTACTTGT ATCAAATCAA 
AATGGAAATA TGTACACATC TTCAGTATAT GGTTCCCTTG CATCTGTTCT AGCACAGTAC 
TCACCTCAGC AATTAGCAGG GAAGAGAATT GGAGTGTTTT CTTATGGTTC TGGTTTGGCT 
GCCACTCTGT ACTCTCTTAA AGTCACACAA GATGCTACAC CGGGGTCTGC TCTTGATAAA 
ATAACAGCAA GTTTATGTGA TCTTAAATCA AGGCTTGATT CAAGAACTGG TGTGGCACCA 
GATGTCTTCG CTGAAAACAT GAAGCTCAGA GAGGACACCC ATCATTTGGT CAACTATATT 
CCCCAGGGTT CAATAGATTC ACTCTTTGAA GGAACGTGGT ACTTAGTTAG GGTGGATGAA 
AAGCACAGAA GAACTTACGC TCGGCGTCCC ACTCCAAATG ATGACACTTT GGATGAAGGA 
GTAGGACTTG TGCATTCAAA CATAGCAACT GAGCATATTC CAAGCCCTGC CAAGAAAGTA 
CCAAGACTCC CTGCCACAGC AGCAGAACCT GAAGCAGCTG TCATTAGTAA TGGGGAACAT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001311148.1
CDS	214..1776
Translation	MPGSLPLNAEACWPKDVGIVALEIYFPSQYVDQAELEKYDGVDAGKYTIGLGQAKMGFCTDREDINSLCMTVVQNLMERNNLSYDCIGRLEVGTETIIDKSKSVKTNLMQLFEESGNTDIEGIDTTNACYGGTAAVFNAVNWIESSSWDGRYALVVAGDIAVYATGNARPTGGVGAVALLIGPNAPLIFERGLRGTHMQHAYDFYKPDMLSEYPIVDGKLSIQCYLSALDRCYSVYCKKIHAQWQKEGNDKDFTLNDFGFMIFHSPYCKLVQKSLARMLLNDFLNDQNRDKNSIYSGLEAFGDVKLEDTYFDRDVEKAFMKASSELFSQKTKASLLVSNQNGNMYTSSVYGSLASVLAQYSPQQLAGKRIGVFSYGSGLAATLYSLKVTQDATPGSALDKITASLCDLKSRLDSRTGVAPDVFAENMKLREDTHHLVNYIPQGSIDSLFEGTWYLVRVDEKHRRTYARRPTPNDDTLDEGVGLVHSNIATEHIPSPAKKVPRLPATAAEPEAAVISNGEH

Target ORF information:

RefSeq Version	NM_001324219.1
Organism	Homo sapiens(human)
Definition	Homo sapiens 3-hydroxy-3-methylglutaryl-CoA synthase 1 (HMGCS1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001324219.1

ORF Insert Sequence:

ATGCCTGGAT CACTTCCTTT GAATGCAGAA GCTTGCTGGC CAAAAGATGT GGGAATTGTT 
GCCCTTGAGA TCTATTTTCC TTCTCAATAT GTTGATCAAG CAGAGTTGGA AAAATATGAT 
GGTGTAGATG CTGGAAAGTA TACCATTGGC TTGGGCCAGG CCAAGATGGG CTTCTGCACA 
GATAGAGAAG ATATTAACTC TCTTTGCATG ACTGTGGTTC AGAATCTTAT GGAGAGAAAT 
AACCTTTCCT ATGATTGCAT TGGGCGGCTG GAAGTTGGAA CAGAGACAAT CATCGACAAA 
TCAAAGTCTG TGAAGACTAA TTTGATGCAG CTGTTTGAAG AGTCTGGGAA TACAGATATA 
GAAGGAATCG ACACAACTAA TGCATGCTAT GGAGGCACAG CTGCTGTCTT CAATGCTGTT 
AACTGGATTG AGTCCAGCTC TTGGGATGGA CGGTATGCCC TGGTAGTTGC AGGAGATATT 
GCTGTATATG CCACAGGAAA TGCTAGACCT ACAGGTGGAG TTGGAGCAGT AGCTCTGCTA 
ATTGGGCCAA ATGCTCCTTT AATTTTTGAA CGAGGGCTTC GTGGGACACA TATGCAACAT 
GCCTATGATT TTTACAAGCC TGATATGCTA TCTGAATATC CTATAGTAGA TGGAAAACTC 
TCCATACAGT GCTACCTCAG TGCATTAGAC CGCTGCTATT CTGTCTACTG CAAAAAGATC 
CATGCCCAGT GGCAGAAAGA GGGAAATGAT AAAGATTTTA CCTTGAATGA TTTTGGCTTC 
ATGATCTTTC ACTCACCATA TTGTAAACTG GTTCAGAAAT CTCTAGCTCG GATGTTGCTG 
AATGACTTCC TTAATGACCA GAATAGAGAT AAAAATAGTA TCTATAGTGG CCTGGAAGCC 
TTTGGGGATG TTAAATTAGA AGACACCTAC TTTGATAGAG ATGTGGAGAA GGCATTTATG 
AAGGCTAGCT CTGAACTCTT CAGTCAGAAA ACAAAGGCAT CTTTACTTGT ATCAAATCAA 
AATGGAAATA TGTACACATC TTCAGTATAT GGTTCCCTTG CATCTGTTCT AGCACAGTAC 
TCACCTCAGC AATTAGCAGG GAAGAGAATT GGAGTGTTTT CTTATGGTTC TGGTTTGGCT 
GCCACTCTGT ACTCTCTTAA AGTCACACAA GATGCTACAC CGGGGTCTGC TCTTGATAAA 
ATAACAGCAA GTTTATGTGA TCTTAAATCA AGGCTTGATT CAAGAACTGG TGTGGCACCA 
GATGTCTTCG CTGAAAACAT GAAGCTCAGA GAGGACACCC ATCATTTGGT CAACTATATT 
CCCCAGGGTT CAATAGATTC ACTCTTTGAA GGAACGTGGT ACTTAGTTAG GGTGGATGAA 
AAGCACAGAA GAACTTACGC TCGGCGTCCC ACTCCAAATG ATGACACTTT GGATGAAGGA 
GTAGGACTTG TGCATTCAAA CATAGCAACT GAGCATATTC CAAGCCCTGC CAAGAAAGTA 
CCAAGACTCC CTGCCACAGC AGCAGAACCT GAAGCAGCTG TCATTAGTAA TGGGGAACAT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HMGCS1 ( NM_001364188.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001351117.1 Homo sapiens 3-hydroxy-3-methylglutaryl-CoA synthase 1 (HMGCS1), transcript variant 9, mRNA.

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CloneID	OHu01309
Clone ID Related Accession (Same CDS sequence)	NM_001324220.1 , NM_001330663.2 , NM_002130.7 , NM_001098272.3 , NM_001330663.1 , NM_001324219.2 , NM_001364188.2 , NM_001324219.1 , NM_001364188.1 , NM_001098272.2 , NM_002130.8 , NM_001324220.2
Accession Version	NM_001364188.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1563bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-27
Organism	Homo sapiens(human)
Product	hydroxymethylglutaryl-CoA synthase, cytoplasmic isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC114947.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.42317.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

ATGCCTGGAT CACTTCCTTT GAATGCAGAA GCTTGCTGGC CAAAAGATGT GGGAATTGTT 
GCCCTTGAGA TCTATTTTCC TTCTCAATAT GTTGATCAAG CAGAGTTGGA AAAATATGAT 
GGTGTAGATG CTGGAAAGTA TACCATTGGC TTGGGCCAGG CCAAGATGGG CTTCTGCACA 
GATAGAGAAG ATATTAACTC TCTTTGCATG ACTGTGGTTC AGAATCTTAT GGAGAGAAAT 
AACCTTTCCT ATGATTGCAT TGGGCGGCTG GAAGTTGGAA CAGAGACAAT CATCGACAAA 
TCAAAGTCTG TGAAGACTAA TTTGATGCAG CTGTTTGAAG AGTCTGGGAA TACAGATATA 
GAAGGAATCG ACACAACTAA TGCATGCTAT GGAGGCACAG CTGCTGTCTT CAATGCTGTT 
AACTGGATTG AGTCCAGCTC TTGGGATGGA CGGTATGCCC TGGTAGTTGC AGGAGATATT 
GCTGTATATG CCACAGGAAA TGCTAGACCT ACAGGTGGAG TTGGAGCAGT AGCTCTGCTA 
ATTGGGCCAA ATGCTCCTTT AATTTTTGAA CGAGGGCTTC GTGGGACACA TATGCAACAT 
GCCTATGATT TTTACAAGCC TGATATGCTA TCTGAATATC CTATAGTAGA TGGAAAACTC 
TCCATACAGT GCTACCTCAG TGCATTAGAC CGCTGCTATT CTGTCTACTG CAAAAAGATC 
CATGCCCAGT GGCAGAAAGA GGGAAATGAT AAAGATTTTA CCTTGAATGA TTTTGGCTTC 
ATGATCTTTC ACTCACCATA TTGTAAACTG GTTCAGAAAT CTCTAGCTCG GATGTTGCTG 
AATGACTTCC TTAATGACCA GAATAGAGAT AAAAATAGTA TCTATAGTGG CCTGGAAGCC 
TTTGGGGATG TTAAATTAGA AGACACCTAC TTTGATAGAG ATGTGGAGAA GGCATTTATG 
AAGGCTAGCT CTGAACTCTT CAGTCAGAAA ACAAAGGCAT CTTTACTTGT ATCAAATCAA 
AATGGAAATA TGTACACATC TTCAGTATAT GGTTCCCTTG CATCTGTTCT AGCACAGTAC 
TCACCTCAGC AATTAGCAGG GAAGAGAATT GGAGTGTTTT CTTATGGTTC TGGTTTGGCT 
GCCACTCTGT ACTCTCTTAA AGTCACACAA GATGCTACAC CGGGGTCTGC TCTTGATAAA 
ATAACAGCAA GTTTATGTGA TCTTAAATCA AGGCTTGATT CAAGAACTGG TGTGGCACCA 
GATGTCTTCG CTGAAAACAT GAAGCTCAGA GAGGACACCC ATCATTTGGT CAACTATATT 
CCCCAGGGTT CAATAGATTC ACTCTTTGAA GGAACGTGGT ACTTAGTTAG GGTGGATGAA 
AAGCACAGAA GAACTTACGC TCGGCGTCCC ACTCCAAATG ATGACACTTT GGATGAAGGA 
GTAGGACTTG TGCATTCAAA CATAGCAACT GAGCATATTC CAAGCCCTGC CAAGAAAGTA 
CCAAGACTCC CTGCCACAGC AGCAGAACCT GAAGCAGCTG TCATTAGTAA TGGGGAACAT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001351117.1
CDS	250..1812
Translation	MPGSLPLNAEACWPKDVGIVALEIYFPSQYVDQAELEKYDGVDAGKYTIGLGQAKMGFCTDREDINSLCMTVVQNLMERNNLSYDCIGRLEVGTETIIDKSKSVKTNLMQLFEESGNTDIEGIDTTNACYGGTAAVFNAVNWIESSSWDGRYALVVAGDIAVYATGNARPTGGVGAVALLIGPNAPLIFERGLRGTHMQHAYDFYKPDMLSEYPIVDGKLSIQCYLSALDRCYSVYCKKIHAQWQKEGNDKDFTLNDFGFMIFHSPYCKLVQKSLARMLLNDFLNDQNRDKNSIYSGLEAFGDVKLEDTYFDRDVEKAFMKASSELFSQKTKASLLVSNQNGNMYTSSVYGSLASVLAQYSPQQLAGKRIGVFSYGSGLAATLYSLKVTQDATPGSALDKITASLCDLKSRLDSRTGVAPDVFAENMKLREDTHHLVNYIPQGSIDSLFEGTWYLVRVDEKHRRTYARRPTPNDDTLDEGVGLVHSNIATEHIPSPAKKVPRLPATAAEPEAAVISNGEH

Target ORF information:

RefSeq Version	NM_001364188.1
Organism	Homo sapiens(human)
Definition	Homo sapiens 3-hydroxy-3-methylglutaryl-CoA synthase 1 (HMGCS1), transcript variant 9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001364188.1

ORF Insert Sequence:

ATGCCTGGAT CACTTCCTTT GAATGCAGAA GCTTGCTGGC CAAAAGATGT GGGAATTGTT 
GCCCTTGAGA TCTATTTTCC TTCTCAATAT GTTGATCAAG CAGAGTTGGA AAAATATGAT 
GGTGTAGATG CTGGAAAGTA TACCATTGGC TTGGGCCAGG CCAAGATGGG CTTCTGCACA 
GATAGAGAAG ATATTAACTC TCTTTGCATG ACTGTGGTTC AGAATCTTAT GGAGAGAAAT 
AACCTTTCCT ATGATTGCAT TGGGCGGCTG GAAGTTGGAA CAGAGACAAT CATCGACAAA 
TCAAAGTCTG TGAAGACTAA TTTGATGCAG CTGTTTGAAG AGTCTGGGAA TACAGATATA 
GAAGGAATCG ACACAACTAA TGCATGCTAT GGAGGCACAG CTGCTGTCTT CAATGCTGTT 
AACTGGATTG AGTCCAGCTC TTGGGATGGA CGGTATGCCC TGGTAGTTGC AGGAGATATT 
GCTGTATATG CCACAGGAAA TGCTAGACCT ACAGGTGGAG TTGGAGCAGT AGCTCTGCTA 
ATTGGGCCAA ATGCTCCTTT AATTTTTGAA CGAGGGCTTC GTGGGACACA TATGCAACAT 
GCCTATGATT TTTACAAGCC TGATATGCTA TCTGAATATC CTATAGTAGA TGGAAAACTC 
TCCATACAGT GCTACCTCAG TGCATTAGAC CGCTGCTATT CTGTCTACTG CAAAAAGATC 
CATGCCCAGT GGCAGAAAGA GGGAAATGAT AAAGATTTTA CCTTGAATGA TTTTGGCTTC 
ATGATCTTTC ACTCACCATA TTGTAAACTG GTTCAGAAAT CTCTAGCTCG GATGTTGCTG 
AATGACTTCC TTAATGACCA GAATAGAGAT AAAAATAGTA TCTATAGTGG CCTGGAAGCC 
TTTGGGGATG TTAAATTAGA AGACACCTAC TTTGATAGAG ATGTGGAGAA GGCATTTATG 
AAGGCTAGCT CTGAACTCTT CAGTCAGAAA ACAAAGGCAT CTTTACTTGT ATCAAATCAA 
AATGGAAATA TGTACACATC TTCAGTATAT GGTTCCCTTG CATCTGTTCT AGCACAGTAC 
TCACCTCAGC AATTAGCAGG GAAGAGAATT GGAGTGTTTT CTTATGGTTC TGGTTTGGCT 
GCCACTCTGT ACTCTCTTAA AGTCACACAA GATGCTACAC CGGGGTCTGC TCTTGATAAA 
ATAACAGCAA GTTTATGTGA TCTTAAATCA AGGCTTGATT CAAGAACTGG TGTGGCACCA 
GATGTCTTCG CTGAAAACAT GAAGCTCAGA GAGGACACCC ATCATTTGGT CAACTATATT 
CCCCAGGGTT CAATAGATTC ACTCTTTGAA GGAACGTGGT ACTTAGTTAG GGTGGATGAA 
AAGCACAGAA GAACTTACGC TCGGCGTCCC ACTCCAAATG ATGACACTTT GGATGAAGGA 
GTAGGACTTG TGCATTCAAA CATAGCAACT GAGCATATTC CAAGCCCTGC CAAGAAAGTA 
CCAAGACTCC CTGCCACAGC AGCAGAACCT GAAGCAGCTG TCATTAGTAA TGGGGAACAT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HMGCS1 ( NM_001098272.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001091742.1 Homo sapiens 3-hydroxy-3-methylglutaryl-CoA synthase 1 (HMGCS1), transcript variant 1, mRNA.

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CloneID	OHu01309
Clone ID Related Accession (Same CDS sequence)	NM_001324220.1 , NM_001330663.2 , NM_002130.7 , NM_001098272.3 , NM_001330663.1 , NM_001324219.2 , NM_001364188.2 , NM_001324219.1 , NM_001364188.1 , NM_001098272.2 , NM_002130.8 , NM_001324220.2
Accession Version	NM_001098272.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1563bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-02
Organism	Homo sapiens(human)
Product	hydroxymethylglutaryl-CoA synthase, cytoplasmic isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DB074885.1, AK315593.1 and AC114947.2. On May 16, 2012 this sequence version replaced NM_001098272.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.11048.1, SRR1660809.42847.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCTGGAT CACTTCCTTT GAATGCAGAA GCTTGCTGGC CAAAAGATGT GGGAATTGTT 
GCCCTTGAGA TCTATTTTCC TTCTCAATAT GTTGATCAAG CAGAGTTGGA AAAATATGAT 
GGTGTAGATG CTGGAAAGTA TACCATTGGC TTGGGCCAGG CCAAGATGGG CTTCTGCACA 
GATAGAGAAG ATATTAACTC TCTTTGCATG ACTGTGGTTC AGAATCTTAT GGAGAGAAAT 
AACCTTTCCT ATGATTGCAT TGGGCGGCTG GAAGTTGGAA CAGAGACAAT CATCGACAAA 
TCAAAGTCTG TGAAGACTAA TTTGATGCAG CTGTTTGAAG AGTCTGGGAA TACAGATATA 
GAAGGAATCG ACACAACTAA TGCATGCTAT GGAGGCACAG CTGCTGTCTT CAATGCTGTT 
AACTGGATTG AGTCCAGCTC TTGGGATGGA CGGTATGCCC TGGTAGTTGC AGGAGATATT 
GCTGTATATG CCACAGGAAA TGCTAGACCT ACAGGTGGAG TTGGAGCAGT AGCTCTGCTA 
ATTGGGCCAA ATGCTCCTTT AATTTTTGAA CGAGGGCTTC GTGGGACACA TATGCAACAT 
GCCTATGATT TTTACAAGCC TGATATGCTA TCTGAATATC CTATAGTAGA TGGAAAACTC 
TCCATACAGT GCTACCTCAG TGCATTAGAC CGCTGCTATT CTGTCTACTG CAAAAAGATC 
CATGCCCAGT GGCAGAAAGA GGGAAATGAT AAAGATTTTA CCTTGAATGA TTTTGGCTTC 
ATGATCTTTC ACTCACCATA TTGTAAACTG GTTCAGAAAT CTCTAGCTCG GATGTTGCTG 
AATGACTTCC TTAATGACCA GAATAGAGAT AAAAATAGTA TCTATAGTGG CCTGGAAGCC 
TTTGGGGATG TTAAATTAGA AGACACCTAC TTTGATAGAG ATGTGGAGAA GGCATTTATG 
AAGGCTAGCT CTGAACTCTT CAGTCAGAAA ACAAAGGCAT CTTTACTTGT ATCAAATCAA 
AATGGAAATA TGTACACATC TTCAGTATAT GGTTCCCTTG CATCTGTTCT AGCACAGTAC 
TCACCTCAGC AATTAGCAGG GAAGAGAATT GGAGTGTTTT CTTATGGTTC TGGTTTGGCT 
GCCACTCTGT ACTCTCTTAA AGTCACACAA GATGCTACAC CGGGGTCTGC TCTTGATAAA 
ATAACAGCAA GTTTATGTGA TCTTAAATCA AGGCTTGATT CAAGAACTGG TGTGGCACCA 
GATGTCTTCG CTGAAAACAT GAAGCTCAGA GAGGACACCC ATCATTTGGT CAACTATATT 
CCCCAGGGTT CAATAGATTC ACTCTTTGAA GGAACGTGGT ACTTAGTTAG GGTGGATGAA 
AAGCACAGAA GAACTTACGC TCGGCGTCCC ACTCCAAATG ATGACACTTT GGATGAAGGA 
GTAGGACTTG TGCATTCAAA CATAGCAACT GAGCATATTC CAAGCCCTGC CAAGAAAGTA 
CCAAGACTCC CTGCCACAGC AGCAGAACCT GAAGCAGCTG TCATTAGTAA TGGGGAACAT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001091742.1
CDS	227..1789
Translation	MPGSLPLNAEACWPKDVGIVALEIYFPSQYVDQAELEKYDGVDAGKYTIGLGQAKMGFCTDREDINSLCMTVVQNLMERNNLSYDCIGRLEVGTETIIDKSKSVKTNLMQLFEESGNTDIEGIDTTNACYGGTAAVFNAVNWIESSSWDGRYALVVAGDIAVYATGNARPTGGVGAVALLIGPNAPLIFERGLRGTHMQHAYDFYKPDMLSEYPIVDGKLSIQCYLSALDRCYSVYCKKIHAQWQKEGNDKDFTLNDFGFMIFHSPYCKLVQKSLARMLLNDFLNDQNRDKNSIYSGLEAFGDVKLEDTYFDRDVEKAFMKASSELFSQKTKASLLVSNQNGNMYTSSVYGSLASVLAQYSPQQLAGKRIGVFSYGSGLAATLYSLKVTQDATPGSALDKITASLCDLKSRLDSRTGVAPDVFAENMKLREDTHHLVNYIPQGSIDSLFEGTWYLVRVDEKHRRTYARRPTPNDDTLDEGVGLVHSNIATEHIPSPAKKVPRLPATAAEPEAAVISNGEH

Target ORF information:

RefSeq Version	NM_001098272.2
Organism	Homo sapiens(human)
Definition	Homo sapiens 3-hydroxy-3-methylglutaryl-CoA synthase 1 (HMGCS1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001098272.2

ORF Insert Sequence:

ATGCCTGGAT CACTTCCTTT GAATGCAGAA GCTTGCTGGC CAAAAGATGT GGGAATTGTT 
GCCCTTGAGA TCTATTTTCC TTCTCAATAT GTTGATCAAG CAGAGTTGGA AAAATATGAT 
GGTGTAGATG CTGGAAAGTA TACCATTGGC TTGGGCCAGG CCAAGATGGG CTTCTGCACA 
GATAGAGAAG ATATTAACTC TCTTTGCATG ACTGTGGTTC AGAATCTTAT GGAGAGAAAT 
AACCTTTCCT ATGATTGCAT TGGGCGGCTG GAAGTTGGAA CAGAGACAAT CATCGACAAA 
TCAAAGTCTG TGAAGACTAA TTTGATGCAG CTGTTTGAAG AGTCTGGGAA TACAGATATA 
GAAGGAATCG ACACAACTAA TGCATGCTAT GGAGGCACAG CTGCTGTCTT CAATGCTGTT 
AACTGGATTG AGTCCAGCTC TTGGGATGGA CGGTATGCCC TGGTAGTTGC AGGAGATATT 
GCTGTATATG CCACAGGAAA TGCTAGACCT ACAGGTGGAG TTGGAGCAGT AGCTCTGCTA 
ATTGGGCCAA ATGCTCCTTT AATTTTTGAA CGAGGGCTTC GTGGGACACA TATGCAACAT 
GCCTATGATT TTTACAAGCC TGATATGCTA TCTGAATATC CTATAGTAGA TGGAAAACTC 
TCCATACAGT GCTACCTCAG TGCATTAGAC CGCTGCTATT CTGTCTACTG CAAAAAGATC 
CATGCCCAGT GGCAGAAAGA GGGAAATGAT AAAGATTTTA CCTTGAATGA TTTTGGCTTC 
ATGATCTTTC ACTCACCATA TTGTAAACTG GTTCAGAAAT CTCTAGCTCG GATGTTGCTG 
AATGACTTCC TTAATGACCA GAATAGAGAT AAAAATAGTA TCTATAGTGG CCTGGAAGCC 
TTTGGGGATG TTAAATTAGA AGACACCTAC TTTGATAGAG ATGTGGAGAA GGCATTTATG 
AAGGCTAGCT CTGAACTCTT CAGTCAGAAA ACAAAGGCAT CTTTACTTGT ATCAAATCAA 
AATGGAAATA TGTACACATC TTCAGTATAT GGTTCCCTTG CATCTGTTCT AGCACAGTAC 
TCACCTCAGC AATTAGCAGG GAAGAGAATT GGAGTGTTTT CTTATGGTTC TGGTTTGGCT 
GCCACTCTGT ACTCTCTTAA AGTCACACAA GATGCTACAC CGGGGTCTGC TCTTGATAAA 
ATAACAGCAA GTTTATGTGA TCTTAAATCA AGGCTTGATT CAAGAACTGG TGTGGCACCA 
GATGTCTTCG CTGAAAACAT GAAGCTCAGA GAGGACACCC ATCATTTGGT CAACTATATT 
CCCCAGGGTT CAATAGATTC ACTCTTTGAA GGAACGTGGT ACTTAGTTAG GGTGGATGAA 
AAGCACAGAA GAACTTACGC TCGGCGTCCC ACTCCAAATG ATGACACTTT GGATGAAGGA 
GTAGGACTTG TGCATTCAAA CATAGCAACT GAGCATATTC CAAGCCCTGC CAAGAAAGTA 
CCAAGACTCC CTGCCACAGC AGCAGAACCT GAAGCAGCTG TCATTAGTAA TGGGGAACAT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HMGCS1 ( NM_002130.8 ) cDNA ORF clone, Homo sapiens(human) -> NP_002121.4 Homo sapiens 3-hydroxy-3-methylglutaryl-CoA synthase 1 (HMGCS1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu01309
Clone ID Related Accession (Same CDS sequence)	NM_001324220.1 , NM_001330663.2 , NM_002130.7 , NM_001098272.3 , NM_001330663.1 , NM_001324219.2 , NM_001364188.2 , NM_001324219.1 , NM_001364188.1 , NM_001098272.2 , NM_002130.8 , NM_001324220.2
Accession Version	NM_002130.8 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1563bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-27
Organism	Homo sapiens(human)
Product	hydroxymethylglutaryl-CoA synthase, cytoplasmic isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC114947.2. On May 31, 2019 this sequence version replaced NM_002130.7. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.92027.1, SRR1660807.236422.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03267761 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCTGGAT CACTTCCTTT GAATGCAGAA GCTTGCTGGC CAAAAGATGT GGGAATTGTT 
GCCCTTGAGA TCTATTTTCC TTCTCAATAT GTTGATCAAG CAGAGTTGGA AAAATATGAT 
GGTGTAGATG CTGGAAAGTA TACCATTGGC TTGGGCCAGG CCAAGATGGG CTTCTGCACA 
GATAGAGAAG ATATTAACTC TCTTTGCATG ACTGTGGTTC AGAATCTTAT GGAGAGAAAT 
AACCTTTCCT ATGATTGCAT TGGGCGGCTG GAAGTTGGAA CAGAGACAAT CATCGACAAA 
TCAAAGTCTG TGAAGACTAA TTTGATGCAG CTGTTTGAAG AGTCTGGGAA TACAGATATA 
GAAGGAATCG ACACAACTAA TGCATGCTAT GGAGGCACAG CTGCTGTCTT CAATGCTGTT 
AACTGGATTG AGTCCAGCTC TTGGGATGGA CGGTATGCCC TGGTAGTTGC AGGAGATATT 
GCTGTATATG CCACAGGAAA TGCTAGACCT ACAGGTGGAG TTGGAGCAGT AGCTCTGCTA 
ATTGGGCCAA ATGCTCCTTT AATTTTTGAA CGAGGGCTTC GTGGGACACA TATGCAACAT 
GCCTATGATT TTTACAAGCC TGATATGCTA TCTGAATATC CTATAGTAGA TGGAAAACTC 
TCCATACAGT GCTACCTCAG TGCATTAGAC CGCTGCTATT CTGTCTACTG CAAAAAGATC 
CATGCCCAGT GGCAGAAAGA GGGAAATGAT AAAGATTTTA CCTTGAATGA TTTTGGCTTC 
ATGATCTTTC ACTCACCATA TTGTAAACTG GTTCAGAAAT CTCTAGCTCG GATGTTGCTG 
AATGACTTCC TTAATGACCA GAATAGAGAT AAAAATAGTA TCTATAGTGG CCTGGAAGCC 
TTTGGGGATG TTAAATTAGA AGACACCTAC TTTGATAGAG ATGTGGAGAA GGCATTTATG 
AAGGCTAGCT CTGAACTCTT CAGTCAGAAA ACAAAGGCAT CTTTACTTGT ATCAAATCAA 
AATGGAAATA TGTACACATC TTCAGTATAT GGTTCCCTTG CATCTGTTCT AGCACAGTAC 
TCACCTCAGC AATTAGCAGG GAAGAGAATT GGAGTGTTTT CTTATGGTTC TGGTTTGGCT 
GCCACTCTGT ACTCTCTTAA AGTCACACAA GATGCTACAC CGGGGTCTGC TCTTGATAAA 
ATAACAGCAA GTTTATGTGA TCTTAAATCA AGGCTTGATT CAAGAACTGG TGTGGCACCA 
GATGTCTTCG CTGAAAACAT GAAGCTCAGA GAGGACACCC ATCATTTGGT CAACTATATT 
CCCCAGGGTT CAATAGATTC ACTCTTTGAA GGAACGTGGT ACTTAGTTAG GGTGGATGAA 
AAGCACAGAA GAACTTACGC TCGGCGTCCC ACTCCAAATG ATGACACTTT GGATGAAGGA 
GTAGGACTTG TGCATTCAAA CATAGCAACT GAGCATATTC CAAGCCCTGC CAAGAAAGTA 
CCAAGACTCC CTGCCACAGC AGCAGAACCT GAAGCAGCTG TCATTAGTAA TGGGGAACAT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002121.4
CDS	68..1630
Translation	MPGSLPLNAEACWPKDVGIVALEIYFPSQYVDQAELEKYDGVDAGKYTIGLGQAKMGFCTDREDINSLCMTVVQNLMERNNLSYDCIGRLEVGTETIIDKSKSVKTNLMQLFEESGNTDIEGIDTTNACYGGTAAVFNAVNWIESSSWDGRYALVVAGDIAVYATGNARPTGGVGAVALLIGPNAPLIFERGLRGTHMQHAYDFYKPDMLSEYPIVDGKLSIQCYLSALDRCYSVYCKKIHAQWQKEGNDKDFTLNDFGFMIFHSPYCKLVQKSLARMLLNDFLNDQNRDKNSIYSGLEAFGDVKLEDTYFDRDVEKAFMKASSELFSQKTKASLLVSNQNGNMYTSSVYGSLASVLAQYSPQQLAGKRIGVFSYGSGLAATLYSLKVTQDATPGSALDKITASLCDLKSRLDSRTGVAPDVFAENMKLREDTHHLVNYIPQGSIDSLFEGTWYLVRVDEKHRRTYARRPTPNDDTLDEGVGLVHSNIATEHIPSPAKKVPRLPATAAEPEAAVISNGEH

Target ORF information:

RefSeq Version	NM_002130.8
Organism	Homo sapiens(human)
Definition	Homo sapiens 3-hydroxy-3-methylglutaryl-CoA synthase 1 (HMGCS1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002130.8

ORF Insert Sequence:

ATGCCTGGAT CACTTCCTTT GAATGCAGAA GCTTGCTGGC CAAAAGATGT GGGAATTGTT 
GCCCTTGAGA TCTATTTTCC TTCTCAATAT GTTGATCAAG CAGAGTTGGA AAAATATGAT 
GGTGTAGATG CTGGAAAGTA TACCATTGGC TTGGGCCAGG CCAAGATGGG CTTCTGCACA 
GATAGAGAAG ATATTAACTC TCTTTGCATG ACTGTGGTTC AGAATCTTAT GGAGAGAAAT 
AACCTTTCCT ATGATTGCAT TGGGCGGCTG GAAGTTGGAA CAGAGACAAT CATCGACAAA 
TCAAAGTCTG TGAAGACTAA TTTGATGCAG CTGTTTGAAG AGTCTGGGAA TACAGATATA 
GAAGGAATCG ACACAACTAA TGCATGCTAT GGAGGCACAG CTGCTGTCTT CAATGCTGTT 
AACTGGATTG AGTCCAGCTC TTGGGATGGA CGGTATGCCC TGGTAGTTGC AGGAGATATT 
GCTGTATATG CCACAGGAAA TGCTAGACCT ACAGGTGGAG TTGGAGCAGT AGCTCTGCTA 
ATTGGGCCAA ATGCTCCTTT AATTTTTGAA CGAGGGCTTC GTGGGACACA TATGCAACAT 
GCCTATGATT TTTACAAGCC TGATATGCTA TCTGAATATC CTATAGTAGA TGGAAAACTC 
TCCATACAGT GCTACCTCAG TGCATTAGAC CGCTGCTATT CTGTCTACTG CAAAAAGATC 
CATGCCCAGT GGCAGAAAGA GGGAAATGAT AAAGATTTTA CCTTGAATGA TTTTGGCTTC 
ATGATCTTTC ACTCACCATA TTGTAAACTG GTTCAGAAAT CTCTAGCTCG GATGTTGCTG 
AATGACTTCC TTAATGACCA GAATAGAGAT AAAAATAGTA TCTATAGTGG CCTGGAAGCC 
TTTGGGGATG TTAAATTAGA AGACACCTAC TTTGATAGAG ATGTGGAGAA GGCATTTATG 
AAGGCTAGCT CTGAACTCTT CAGTCAGAAA ACAAAGGCAT CTTTACTTGT ATCAAATCAA 
AATGGAAATA TGTACACATC TTCAGTATAT GGTTCCCTTG CATCTGTTCT AGCACAGTAC 
TCACCTCAGC AATTAGCAGG GAAGAGAATT GGAGTGTTTT CTTATGGTTC TGGTTTGGCT 
GCCACTCTGT ACTCTCTTAA AGTCACACAA GATGCTACAC CGGGGTCTGC TCTTGATAAA 
ATAACAGCAA GTTTATGTGA TCTTAAATCA AGGCTTGATT CAAGAACTGG TGTGGCACCA 
GATGTCTTCG CTGAAAACAT GAAGCTCAGA GAGGACACCC ATCATTTGGT CAACTATATT 
CCCCAGGGTT CAATAGATTC ACTCTTTGAA GGAACGTGGT ACTTAGTTAG GGTGGATGAA 
AAGCACAGAA GAACTTACGC TCGGCGTCCC ACTCCAAATG ATGACACTTT GGATGAAGGA 
GTAGGACTTG TGCATTCAAA CATAGCAACT GAGCATATTC CAAGCCCTGC CAAGAAAGTA 
CCAAGACTCC CTGCCACAGC AGCAGAACCT GAAGCAGCTG TCATTAGTAA TGGGGAACAT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HMGCS1 ( NM_001324220.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001311149.1 Homo sapiens 3-hydroxy-3-methylglutaryl-CoA synthase 1 (HMGCS1), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu01309
Clone ID Related Accession (Same CDS sequence)	NM_001324220.1 , NM_001330663.2 , NM_002130.7 , NM_001098272.3 , NM_001330663.1 , NM_001324219.2 , NM_001364188.2 , NM_001324219.1 , NM_001364188.1 , NM_001098272.2 , NM_002130.8 , NM_001324220.2
Accession Version	NM_001324220.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1563bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-28
Organism	Homo sapiens(human)
Product	hydroxymethylglutaryl-CoA synthase, cytoplasmic isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BJ995153.1, AK315593.1 and AC114947.2. On Jun 1, 2019 this sequence version replaced NM_001324220.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.163703.1, SRR1660803.231163.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCTGGAT CACTTCCTTT GAATGCAGAA GCTTGCTGGC CAAAAGATGT GGGAATTGTT 
GCCCTTGAGA TCTATTTTCC TTCTCAATAT GTTGATCAAG CAGAGTTGGA AAAATATGAT 
GGTGTAGATG CTGGAAAGTA TACCATTGGC TTGGGCCAGG CCAAGATGGG CTTCTGCACA 
GATAGAGAAG ATATTAACTC TCTTTGCATG ACTGTGGTTC AGAATCTTAT GGAGAGAAAT 
AACCTTTCCT ATGATTGCAT TGGGCGGCTG GAAGTTGGAA CAGAGACAAT CATCGACAAA 
TCAAAGTCTG TGAAGACTAA TTTGATGCAG CTGTTTGAAG AGTCTGGGAA TACAGATATA 
GAAGGAATCG ACACAACTAA TGCATGCTAT GGAGGCACAG CTGCTGTCTT CAATGCTGTT 
AACTGGATTG AGTCCAGCTC TTGGGATGGA CGGTATGCCC TGGTAGTTGC AGGAGATATT 
GCTGTATATG CCACAGGAAA TGCTAGACCT ACAGGTGGAG TTGGAGCAGT AGCTCTGCTA 
ATTGGGCCAA ATGCTCCTTT AATTTTTGAA CGAGGGCTTC GTGGGACACA TATGCAACAT 
GCCTATGATT TTTACAAGCC TGATATGCTA TCTGAATATC CTATAGTAGA TGGAAAACTC 
TCCATACAGT GCTACCTCAG TGCATTAGAC CGCTGCTATT CTGTCTACTG CAAAAAGATC 
CATGCCCAGT GGCAGAAAGA GGGAAATGAT AAAGATTTTA CCTTGAATGA TTTTGGCTTC 
ATGATCTTTC ACTCACCATA TTGTAAACTG GTTCAGAAAT CTCTAGCTCG GATGTTGCTG 
AATGACTTCC TTAATGACCA GAATAGAGAT AAAAATAGTA TCTATAGTGG CCTGGAAGCC 
TTTGGGGATG TTAAATTAGA AGACACCTAC TTTGATAGAG ATGTGGAGAA GGCATTTATG 
AAGGCTAGCT CTGAACTCTT CAGTCAGAAA ACAAAGGCAT CTTTACTTGT ATCAAATCAA 
AATGGAAATA TGTACACATC TTCAGTATAT GGTTCCCTTG CATCTGTTCT AGCACAGTAC 
TCACCTCAGC AATTAGCAGG GAAGAGAATT GGAGTGTTTT CTTATGGTTC TGGTTTGGCT 
GCCACTCTGT ACTCTCTTAA AGTCACACAA GATGCTACAC CGGGGTCTGC TCTTGATAAA 
ATAACAGCAA GTTTATGTGA TCTTAAATCA AGGCTTGATT CAAGAACTGG TGTGGCACCA 
GATGTCTTCG CTGAAAACAT GAAGCTCAGA GAGGACACCC ATCATTTGGT CAACTATATT 
CCCCAGGGTT CAATAGATTC ACTCTTTGAA GGAACGTGGT ACTTAGTTAG GGTGGATGAA 
AAGCACAGAA GAACTTACGC TCGGCGTCCC ACTCCAAATG ATGACACTTT GGATGAAGGA 
GTAGGACTTG TGCATTCAAA CATAGCAACT GAGCATATTC CAAGCCCTGC CAAGAAAGTA 
CCAAGACTCC CTGCCACAGC AGCAGAACCT GAAGCAGCTG TCATTAGTAA TGGGGAACAT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001311149.1
CDS	147..1709
Translation	MPGSLPLNAEACWPKDVGIVALEIYFPSQYVDQAELEKYDGVDAGKYTIGLGQAKMGFCTDREDINSLCMTVVQNLMERNNLSYDCIGRLEVGTETIIDKSKSVKTNLMQLFEESGNTDIEGIDTTNACYGGTAAVFNAVNWIESSSWDGRYALVVAGDIAVYATGNARPTGGVGAVALLIGPNAPLIFERGLRGTHMQHAYDFYKPDMLSEYPIVDGKLSIQCYLSALDRCYSVYCKKIHAQWQKEGNDKDFTLNDFGFMIFHSPYCKLVQKSLARMLLNDFLNDQNRDKNSIYSGLEAFGDVKLEDTYFDRDVEKAFMKASSELFSQKTKASLLVSNQNGNMYTSSVYGSLASVLAQYSPQQLAGKRIGVFSYGSGLAATLYSLKVTQDATPGSALDKITASLCDLKSRLDSRTGVAPDVFAENMKLREDTHHLVNYIPQGSIDSLFEGTWYLVRVDEKHRRTYARRPTPNDDTLDEGVGLVHSNIATEHIPSPAKKVPRLPATAAEPEAAVISNGEH

Target ORF information:

RefSeq Version	NM_001324220.2
Organism	Homo sapiens(human)
Definition	Homo sapiens 3-hydroxy-3-methylglutaryl-CoA synthase 1 (HMGCS1), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001324220.2

ORF Insert Sequence:

ATGCCTGGAT CACTTCCTTT GAATGCAGAA GCTTGCTGGC CAAAAGATGT GGGAATTGTT 
GCCCTTGAGA TCTATTTTCC TTCTCAATAT GTTGATCAAG CAGAGTTGGA AAAATATGAT 
GGTGTAGATG CTGGAAAGTA TACCATTGGC TTGGGCCAGG CCAAGATGGG CTTCTGCACA 
GATAGAGAAG ATATTAACTC TCTTTGCATG ACTGTGGTTC AGAATCTTAT GGAGAGAAAT 
AACCTTTCCT ATGATTGCAT TGGGCGGCTG GAAGTTGGAA CAGAGACAAT CATCGACAAA 
TCAAAGTCTG TGAAGACTAA TTTGATGCAG CTGTTTGAAG AGTCTGGGAA TACAGATATA 
GAAGGAATCG ACACAACTAA TGCATGCTAT GGAGGCACAG CTGCTGTCTT CAATGCTGTT 
AACTGGATTG AGTCCAGCTC TTGGGATGGA CGGTATGCCC TGGTAGTTGC AGGAGATATT 
GCTGTATATG CCACAGGAAA TGCTAGACCT ACAGGTGGAG TTGGAGCAGT AGCTCTGCTA 
ATTGGGCCAA ATGCTCCTTT AATTTTTGAA CGAGGGCTTC GTGGGACACA TATGCAACAT 
GCCTATGATT TTTACAAGCC TGATATGCTA TCTGAATATC CTATAGTAGA TGGAAAACTC 
TCCATACAGT GCTACCTCAG TGCATTAGAC CGCTGCTATT CTGTCTACTG CAAAAAGATC 
CATGCCCAGT GGCAGAAAGA GGGAAATGAT AAAGATTTTA CCTTGAATGA TTTTGGCTTC 
ATGATCTTTC ACTCACCATA TTGTAAACTG GTTCAGAAAT CTCTAGCTCG GATGTTGCTG 
AATGACTTCC TTAATGACCA GAATAGAGAT AAAAATAGTA TCTATAGTGG CCTGGAAGCC 
TTTGGGGATG TTAAATTAGA AGACACCTAC TTTGATAGAG ATGTGGAGAA GGCATTTATG 
AAGGCTAGCT CTGAACTCTT CAGTCAGAAA ACAAAGGCAT CTTTACTTGT ATCAAATCAA 
AATGGAAATA TGTACACATC TTCAGTATAT GGTTCCCTTG CATCTGTTCT AGCACAGTAC 
TCACCTCAGC AATTAGCAGG GAAGAGAATT GGAGTGTTTT CTTATGGTTC TGGTTTGGCT 
GCCACTCTGT ACTCTCTTAA AGTCACACAA GATGCTACAC CGGGGTCTGC TCTTGATAAA 
ATAACAGCAA GTTTATGTGA TCTTAAATCA AGGCTTGATT CAAGAACTGG TGTGGCACCA 
GATGTCTTCG CTGAAAACAT GAAGCTCAGA GAGGACACCC ATCATTTGGT CAACTATATT 
CCCCAGGGTT CAATAGATTC ACTCTTTGAA GGAACGTGGT ACTTAGTTAG GGTGGATGAA 
AAGCACAGAA GAACTTACGC TCGGCGTCCC ACTCCAAATG ATGACACTTT GGATGAAGGA 
GTAGGACTTG TGCATTCAAA CATAGCAACT GAGCATATTC CAAGCCCTGC CAAGAAAGTA 
CCAAGACTCC CTGCCACAGC AGCAGAACCT GAAGCAGCTG TCATTAGTAA TGGGGAACAT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HMGCS1 ( NM_001324222.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001311151.1 Homo sapiens 3-hydroxy-3-methylglutaryl-CoA synthase 1 (HMGCS1), transcript variant 5, mRNA.

Price & Availability↑

CloneID	OHu39317
Clone ID Related Accession (Same CDS sequence)	NM_001324222.2 , NM_001324224.1 , NM_001324222.1 , NM_001324223.2 , NM_001324224.2 , NM_001324223.1
Accession Version	NM_001324222.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1437bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-27
Organism	Homo sapiens(human)
Product	hydroxymethylglutaryl-CoA synthase, cytoplasmic isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK315593.1 and AC114947.2. On Jun 1, 2019 this sequence version replaced NM_001324222.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.84804.1, SRR1803613.16109.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCTGGAT CACTTCCTTT GAATGCAGAA GCTTGCTGGC CAAAAGATGT GGGAATTGTT 
GCCCTTGAGA TCTATTTTCC TTCTCAATAT GTTGATCAAG CAGAGTTGGA AAAATATGAT 
GGTGTAGATG CTGGAAAGTA TACCATTGGC TTGGGCCAGG CCAAGATGGG CTTCTGCACA 
GATAGAGAAG ATATTAACTC TCTTTGCATG ACTGTGGTTC AGAATCTTAT GGAGAGAAAT 
AACCTTTCCT ATGATTGCAT TGGGCGGCTG GAAGTTGGAA CAGAGACAAT CATCGACAAA 
TCAAAGTCTG TGAAGACTAA TTTGATGCAG CTGTTTGAAG AGTCTGGGAA TACAGATATA 
GAAGGAATCG ACACAACTAA TGCATGCTAT GGAGGCACAG CTGCTGTCTT CAATGCTGTT 
AACTGGATTG AGTCCAGCTC TTGGGATGGG CTTCGTGGGA CACATATGCA ACATGCCTAT 
GATTTTTACA AGCCTGATAT GCTATCTGAA TATCCTATAG TAGATGGAAA ACTCTCCATA 
CAGTGCTACC TCAGTGCATT AGACCGCTGC TATTCTGTCT ACTGCAAAAA GATCCATGCC 
CAGTGGCAGA AAGAGGGAAA TGATAAAGAT TTTACCTTGA ATGATTTTGG CTTCATGATC 
TTTCACTCAC CATATTGTAA ACTGGTTCAG AAATCTCTAG CTCGGATGTT GCTGAATGAC 
TTCCTTAATG ACCAGAATAG AGATAAAAAT AGTATCTATA GTGGCCTGGA AGCCTTTGGG 
GATGTTAAAT TAGAAGACAC CTACTTTGAT AGAGATGTGG AGAAGGCATT TATGAAGGCT 
AGCTCTGAAC TCTTCAGTCA GAAAACAAAG GCATCTTTAC TTGTATCAAA TCAAAATGGA 
AATATGTACA CATCTTCAGT ATATGGTTCC CTTGCATCTG TTCTAGCACA GTACTCACCT 
CAGCAATTAG CAGGGAAGAG AATTGGAGTG TTTTCTTATG GTTCTGGTTT GGCTGCCACT 
CTGTACTCTC TTAAAGTCAC ACAAGATGCT ACACCGGGGT CTGCTCTTGA TAAAATAACA 
GCAAGTTTAT GTGATCTTAA ATCAAGGCTT GATTCAAGAA CTGGTGTGGC ACCAGATGTC 
TTCGCTGAAA ACATGAAGCT CAGAGAGGAC ACCCATCATT TGGTCAACTA TATTCCCCAG 
GGTTCAATAG ATTCACTCTT TGAAGGAACG TGGTACTTAG TTAGGGTGGA TGAAAAGCAC 
AGAAGAACTT ACGCTCGGCG TCCCACTCCA AATGATGACA CTTTGGATGA AGGAGTAGGA 
CTTGTGCATT CAAACATAGC AACTGAGCAT ATTCCAAGCC CTGCCAAGAA AGTACCAAGA 
CTCCCTGCCA CAGCAGCAGA ACCTGAAGCA GCTGTCATTA GTAATGGGGA ACATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001311151.1
CDS	127..1563
Translation	MPGSLPLNAEACWPKDVGIVALEIYFPSQYVDQAELEKYDGVDAGKYTIGLGQAKMGFCTDREDINSLCMTVVQNLMERNNLSYDCIGRLEVGTETIIDKSKSVKTNLMQLFEESGNTDIEGIDTTNACYGGTAAVFNAVNWIESSSWDGLRGTHMQHAYDFYKPDMLSEYPIVDGKLSIQCYLSALDRCYSVYCKKIHAQWQKEGNDKDFTLNDFGFMIFHSPYCKLVQKSLARMLLNDFLNDQNRDKNSIYSGLEAFGDVKLEDTYFDRDVEKAFMKASSELFSQKTKASLLVSNQNGNMYTSSVYGSLASVLAQYSPQQLAGKRIGVFSYGSGLAATLYSLKVTQDATPGSALDKITASLCDLKSRLDSRTGVAPDVFAENMKLREDTHHLVNYIPQGSIDSLFEGTWYLVRVDEKHRRTYARRPTPNDDTLDEGVGLVHSNIATEHIPSPAKKVPRLPATAAEPEAAVISNGEH

Target ORF information:

RefSeq Version	NM_001324222.2
Organism	Homo sapiens(human)
Definition	Homo sapiens 3-hydroxy-3-methylglutaryl-CoA synthase 1 (HMGCS1), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001324222.2

ORF Insert Sequence:

ATGCCTGGAT CACTTCCTTT GAATGCAGAA GCTTGCTGGC CAAAAGATGT GGGAATTGTT 
GCCCTTGAGA TCTATTTTCC TTCTCAATAT GTTGATCAAG CAGAGTTGGA AAAATATGAT 
GGTGTAGATG CTGGAAAGTA TACCATTGGC TTGGGCCAGG CCAAGATGGG CTTCTGCACA 
GATAGAGAAG ATATTAACTC TCTTTGCATG ACTGTGGTTC AGAATCTTAT GGAGAGAAAT 
AACCTTTCCT ATGATTGCAT TGGGCGGCTG GAAGTTGGAA CAGAGACAAT CATCGACAAA 
TCAAAGTCTG TGAAGACTAA TTTGATGCAG CTGTTTGAAG AGTCTGGGAA TACAGATATA 
GAAGGAATCG ACACAACTAA TGCATGCTAT GGAGGCACAG CTGCTGTCTT CAATGCTGTT 
AACTGGATTG AGTCCAGCTC TTGGGATGGG CTTCGTGGGA CACATATGCA ACATGCCTAT 
GATTTTTACA AGCCTGATAT GCTATCTGAA TATCCTATAG TAGATGGAAA ACTCTCCATA 
CAGTGCTACC TCAGTGCATT AGACCGCTGC TATTCTGTCT ACTGCAAAAA GATCCATGCC 
CAGTGGCAGA AAGAGGGAAA TGATAAAGAT TTTACCTTGA ATGATTTTGG CTTCATGATC 
TTTCACTCAC CATATTGTAA ACTGGTTCAG AAATCTCTAG CTCGGATGTT GCTGAATGAC 
TTCCTTAATG ACCAGAATAG AGATAAAAAT AGTATCTATA GTGGCCTGGA AGCCTTTGGG 
GATGTTAAAT TAGAAGACAC CTACTTTGAT AGAGATGTGG AGAAGGCATT TATGAAGGCT 
AGCTCTGAAC TCTTCAGTCA GAAAACAAAG GCATCTTTAC TTGTATCAAA TCAAAATGGA 
AATATGTACA CATCTTCAGT ATATGGTTCC CTTGCATCTG TTCTAGCACA GTACTCACCT 
CAGCAATTAG CAGGGAAGAG AATTGGAGTG TTTTCTTATG GTTCTGGTTT GGCTGCCACT 
CTGTACTCTC TTAAAGTCAC ACAAGATGCT ACACCGGGGT CTGCTCTTGA TAAAATAACA 
GCAAGTTTAT GTGATCTTAA ATCAAGGCTT GATTCAAGAA CTGGTGTGGC ACCAGATGTC 
TTCGCTGAAA ACATGAAGCT CAGAGAGGAC ACCCATCATT TGGTCAACTA TATTCCCCAG 
GGTTCAATAG ATTCACTCTT TGAAGGAACG TGGTACTTAG TTAGGGTGGA TGAAAAGCAC 
AGAAGAACTT ACGCTCGGCG TCCCACTCCA AATGATGACA CTTTGGATGA AGGAGTAGGA 
CTTGTGCATT CAAACATAGC AACTGAGCAT ATTCCAAGCC CTGCCAAGAA AGTACCAAGA 
CTCCCTGCCA CAGCAGCAGA ACCTGAAGCA GCTGTCATTA GTAATGGGGA ACATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HMGCS1 ( NM_001324224.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001311153.1 Homo sapiens 3-hydroxy-3-methylglutaryl-CoA synthase 1 (HMGCS1), transcript variant 7, mRNA.

Price & Availability↑

CloneID	OHu39317
Clone ID Related Accession (Same CDS sequence)	NM_001324222.2 , NM_001324224.1 , NM_001324222.1 , NM_001324223.2 , NM_001324224.2 , NM_001324223.1
Accession Version	NM_001324224.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1437bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-24
Organism	Homo sapiens(human)
Product	hydroxymethylglutaryl-CoA synthase, cytoplasmic isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DB074885.1, BJ995153.1, AK315593.1 and AC114947.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.92218.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCTGGAT CACTTCCTTT GAATGCAGAA GCTTGCTGGC CAAAAGATGT GGGAATTGTT 
GCCCTTGAGA TCTATTTTCC TTCTCAATAT GTTGATCAAG CAGAGTTGGA AAAATATGAT 
GGTGTAGATG CTGGAAAGTA TACCATTGGC TTGGGCCAGG CCAAGATGGG CTTCTGCACA 
GATAGAGAAG ATATTAACTC TCTTTGCATG ACTGTGGTTC AGAATCTTAT GGAGAGAAAT 
AACCTTTCCT ATGATTGCAT TGGGCGGCTG GAAGTTGGAA CAGAGACAAT CATCGACAAA 
TCAAAGTCTG TGAAGACTAA TTTGATGCAG CTGTTTGAAG AGTCTGGGAA TACAGATATA 
GAAGGAATCG ACACAACTAA TGCATGCTAT GGAGGCACAG CTGCTGTCTT CAATGCTGTT 
AACTGGATTG AGTCCAGCTC TTGGGATGGG CTTCGTGGGA CACATATGCA ACATGCCTAT 
GATTTTTACA AGCCTGATAT GCTATCTGAA TATCCTATAG TAGATGGAAA ACTCTCCATA 
CAGTGCTACC TCAGTGCATT AGACCGCTGC TATTCTGTCT ACTGCAAAAA GATCCATGCC 
CAGTGGCAGA AAGAGGGAAA TGATAAAGAT TTTACCTTGA ATGATTTTGG CTTCATGATC 
TTTCACTCAC CATATTGTAA ACTGGTTCAG AAATCTCTAG CTCGGATGTT GCTGAATGAC 
TTCCTTAATG ACCAGAATAG AGATAAAAAT AGTATCTATA GTGGCCTGGA AGCCTTTGGG 
GATGTTAAAT TAGAAGACAC CTACTTTGAT AGAGATGTGG AGAAGGCATT TATGAAGGCT 
AGCTCTGAAC TCTTCAGTCA GAAAACAAAG GCATCTTTAC TTGTATCAAA TCAAAATGGA 
AATATGTACA CATCTTCAGT ATATGGTTCC CTTGCATCTG TTCTAGCACA GTACTCACCT 
CAGCAATTAG CAGGGAAGAG AATTGGAGTG TTTTCTTATG GTTCTGGTTT GGCTGCCACT 
CTGTACTCTC TTAAAGTCAC ACAAGATGCT ACACCGGGGT CTGCTCTTGA TAAAATAACA 
GCAAGTTTAT GTGATCTTAA ATCAAGGCTT GATTCAAGAA CTGGTGTGGC ACCAGATGTC 
TTCGCTGAAA ACATGAAGCT CAGAGAGGAC ACCCATCATT TGGTCAACTA TATTCCCCAG 
GGTTCAATAG ATTCACTCTT TGAAGGAACG TGGTACTTAG TTAGGGTGGA TGAAAAGCAC 
AGAAGAACTT ACGCTCGGCG TCCCACTCCA AATGATGACA CTTTGGATGA AGGAGTAGGA 
CTTGTGCATT CAAACATAGC AACTGAGCAT ATTCCAAGCC CTGCCAAGAA AGTACCAAGA 
CTCCCTGCCA CAGCAGCAGA ACCTGAAGCA GCTGTCATTA GTAATGGGGA ACATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001311153.1
CDS	247..1683
Translation	MPGSLPLNAEACWPKDVGIVALEIYFPSQYVDQAELEKYDGVDAGKYTIGLGQAKMGFCTDREDINSLCMTVVQNLMERNNLSYDCIGRLEVGTETIIDKSKSVKTNLMQLFEESGNTDIEGIDTTNACYGGTAAVFNAVNWIESSSWDGLRGTHMQHAYDFYKPDMLSEYPIVDGKLSIQCYLSALDRCYSVYCKKIHAQWQKEGNDKDFTLNDFGFMIFHSPYCKLVQKSLARMLLNDFLNDQNRDKNSIYSGLEAFGDVKLEDTYFDRDVEKAFMKASSELFSQKTKASLLVSNQNGNMYTSSVYGSLASVLAQYSPQQLAGKRIGVFSYGSGLAATLYSLKVTQDATPGSALDKITASLCDLKSRLDSRTGVAPDVFAENMKLREDTHHLVNYIPQGSIDSLFEGTWYLVRVDEKHRRTYARRPTPNDDTLDEGVGLVHSNIATEHIPSPAKKVPRLPATAAEPEAAVISNGEH

Target ORF information:

RefSeq Version	NM_001324224.1
Organism	Homo sapiens(human)
Definition	Homo sapiens 3-hydroxy-3-methylglutaryl-CoA synthase 1 (HMGCS1), transcript variant 7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001324224.1

ORF Insert Sequence:

ATGCCTGGAT CACTTCCTTT GAATGCAGAA GCTTGCTGGC CAAAAGATGT GGGAATTGTT 
GCCCTTGAGA TCTATTTTCC TTCTCAATAT GTTGATCAAG CAGAGTTGGA AAAATATGAT 
GGTGTAGATG CTGGAAAGTA TACCATTGGC TTGGGCCAGG CCAAGATGGG CTTCTGCACA 
GATAGAGAAG ATATTAACTC TCTTTGCATG ACTGTGGTTC AGAATCTTAT GGAGAGAAAT 
AACCTTTCCT ATGATTGCAT TGGGCGGCTG GAAGTTGGAA CAGAGACAAT CATCGACAAA 
TCAAAGTCTG TGAAGACTAA TTTGATGCAG CTGTTTGAAG AGTCTGGGAA TACAGATATA 
GAAGGAATCG ACACAACTAA TGCATGCTAT GGAGGCACAG CTGCTGTCTT CAATGCTGTT 
AACTGGATTG AGTCCAGCTC TTGGGATGGG CTTCGTGGGA CACATATGCA ACATGCCTAT 
GATTTTTACA AGCCTGATAT GCTATCTGAA TATCCTATAG TAGATGGAAA ACTCTCCATA 
CAGTGCTACC TCAGTGCATT AGACCGCTGC TATTCTGTCT ACTGCAAAAA GATCCATGCC 
CAGTGGCAGA AAGAGGGAAA TGATAAAGAT TTTACCTTGA ATGATTTTGG CTTCATGATC 
TTTCACTCAC CATATTGTAA ACTGGTTCAG AAATCTCTAG CTCGGATGTT GCTGAATGAC 
TTCCTTAATG ACCAGAATAG AGATAAAAAT AGTATCTATA GTGGCCTGGA AGCCTTTGGG 
GATGTTAAAT TAGAAGACAC CTACTTTGAT AGAGATGTGG AGAAGGCATT TATGAAGGCT 
AGCTCTGAAC TCTTCAGTCA GAAAACAAAG GCATCTTTAC TTGTATCAAA TCAAAATGGA 
AATATGTACA CATCTTCAGT ATATGGTTCC CTTGCATCTG TTCTAGCACA GTACTCACCT 
CAGCAATTAG CAGGGAAGAG AATTGGAGTG TTTTCTTATG GTTCTGGTTT GGCTGCCACT 
CTGTACTCTC TTAAAGTCAC ACAAGATGCT ACACCGGGGT CTGCTCTTGA TAAAATAACA 
GCAAGTTTAT GTGATCTTAA ATCAAGGCTT GATTCAAGAA CTGGTGTGGC ACCAGATGTC 
TTCGCTGAAA ACATGAAGCT CAGAGAGGAC ACCCATCATT TGGTCAACTA TATTCCCCAG 
GGTTCAATAG ATTCACTCTT TGAAGGAACG TGGTACTTAG TTAGGGTGGA TGAAAAGCAC 
AGAAGAACTT ACGCTCGGCG TCCCACTCCA AATGATGACA CTTTGGATGA AGGAGTAGGA 
CTTGTGCATT CAAACATAGC AACTGAGCAT ATTCCAAGCC CTGCCAAGAA AGTACCAAGA 
CTCCCTGCCA CAGCAGCAGA ACCTGAAGCA GCTGTCATTA GTAATGGGGA ACATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HMGCS1 ( NM_001324222.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001311151.1 Homo sapiens 3-hydroxy-3-methylglutaryl-CoA synthase 1 (HMGCS1), transcript variant 5, mRNA.

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CloneID	OHu39317
Clone ID Related Accession (Same CDS sequence)	NM_001324222.2 , NM_001324224.1 , NM_001324222.1 , NM_001324223.2 , NM_001324224.2 , NM_001324223.1
Accession Version	NM_001324222.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1437bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-24
Organism	Homo sapiens(human)
Product	hydroxymethylglutaryl-CoA synthase, cytoplasmic isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DB074885.1, AK315593.1 and AC114947.2. On Apr 28, 2016 this sequence version replaced XM_011514035.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.84804.1, SRR1803613.16109.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCTGGAT CACTTCCTTT GAATGCAGAA GCTTGCTGGC CAAAAGATGT GGGAATTGTT 
GCCCTTGAGA TCTATTTTCC TTCTCAATAT GTTGATCAAG CAGAGTTGGA AAAATATGAT 
GGTGTAGATG CTGGAAAGTA TACCATTGGC TTGGGCCAGG CCAAGATGGG CTTCTGCACA 
GATAGAGAAG ATATTAACTC TCTTTGCATG ACTGTGGTTC AGAATCTTAT GGAGAGAAAT 
AACCTTTCCT ATGATTGCAT TGGGCGGCTG GAAGTTGGAA CAGAGACAAT CATCGACAAA 
TCAAAGTCTG TGAAGACTAA TTTGATGCAG CTGTTTGAAG AGTCTGGGAA TACAGATATA 
GAAGGAATCG ACACAACTAA TGCATGCTAT GGAGGCACAG CTGCTGTCTT CAATGCTGTT 
AACTGGATTG AGTCCAGCTC TTGGGATGGG CTTCGTGGGA CACATATGCA ACATGCCTAT 
GATTTTTACA AGCCTGATAT GCTATCTGAA TATCCTATAG TAGATGGAAA ACTCTCCATA 
CAGTGCTACC TCAGTGCATT AGACCGCTGC TATTCTGTCT ACTGCAAAAA GATCCATGCC 
CAGTGGCAGA AAGAGGGAAA TGATAAAGAT TTTACCTTGA ATGATTTTGG CTTCATGATC 
TTTCACTCAC CATATTGTAA ACTGGTTCAG AAATCTCTAG CTCGGATGTT GCTGAATGAC 
TTCCTTAATG ACCAGAATAG AGATAAAAAT AGTATCTATA GTGGCCTGGA AGCCTTTGGG 
GATGTTAAAT TAGAAGACAC CTACTTTGAT AGAGATGTGG AGAAGGCATT TATGAAGGCT 
AGCTCTGAAC TCTTCAGTCA GAAAACAAAG GCATCTTTAC TTGTATCAAA TCAAAATGGA 
AATATGTACA CATCTTCAGT ATATGGTTCC CTTGCATCTG TTCTAGCACA GTACTCACCT 
CAGCAATTAG CAGGGAAGAG AATTGGAGTG TTTTCTTATG GTTCTGGTTT GGCTGCCACT 
CTGTACTCTC TTAAAGTCAC ACAAGATGCT ACACCGGGGT CTGCTCTTGA TAAAATAACA 
GCAAGTTTAT GTGATCTTAA ATCAAGGCTT GATTCAAGAA CTGGTGTGGC ACCAGATGTC 
TTCGCTGAAA ACATGAAGCT CAGAGAGGAC ACCCATCATT TGGTCAACTA TATTCCCCAG 
GGTTCAATAG ATTCACTCTT TGAAGGAACG TGGTACTTAG TTAGGGTGGA TGAAAAGCAC 
AGAAGAACTT ACGCTCGGCG TCCCACTCCA AATGATGACA CTTTGGATGA AGGAGTAGGA 
CTTGTGCATT CAAACATAGC AACTGAGCAT ATTCCAAGCC CTGCCAAGAA AGTACCAAGA 
CTCCCTGCCA CAGCAGCAGA ACCTGAAGCA GCTGTCATTA GTAATGGGGA ACATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001311151.1
CDS	227..1663
Translation	MPGSLPLNAEACWPKDVGIVALEIYFPSQYVDQAELEKYDGVDAGKYTIGLGQAKMGFCTDREDINSLCMTVVQNLMERNNLSYDCIGRLEVGTETIIDKSKSVKTNLMQLFEESGNTDIEGIDTTNACYGGTAAVFNAVNWIESSSWDGLRGTHMQHAYDFYKPDMLSEYPIVDGKLSIQCYLSALDRCYSVYCKKIHAQWQKEGNDKDFTLNDFGFMIFHSPYCKLVQKSLARMLLNDFLNDQNRDKNSIYSGLEAFGDVKLEDTYFDRDVEKAFMKASSELFSQKTKASLLVSNQNGNMYTSSVYGSLASVLAQYSPQQLAGKRIGVFSYGSGLAATLYSLKVTQDATPGSALDKITASLCDLKSRLDSRTGVAPDVFAENMKLREDTHHLVNYIPQGSIDSLFEGTWYLVRVDEKHRRTYARRPTPNDDTLDEGVGLVHSNIATEHIPSPAKKVPRLPATAAEPEAAVISNGEH

Target ORF information:

RefSeq Version	NM_001324222.1
Organism	Homo sapiens(human)
Definition	Homo sapiens 3-hydroxy-3-methylglutaryl-CoA synthase 1 (HMGCS1), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001324222.1

ORF Insert Sequence:

ATGCCTGGAT CACTTCCTTT GAATGCAGAA GCTTGCTGGC CAAAAGATGT GGGAATTGTT 
GCCCTTGAGA TCTATTTTCC TTCTCAATAT GTTGATCAAG CAGAGTTGGA AAAATATGAT 
GGTGTAGATG CTGGAAAGTA TACCATTGGC TTGGGCCAGG CCAAGATGGG CTTCTGCACA 
GATAGAGAAG ATATTAACTC TCTTTGCATG ACTGTGGTTC AGAATCTTAT GGAGAGAAAT 
AACCTTTCCT ATGATTGCAT TGGGCGGCTG GAAGTTGGAA CAGAGACAAT CATCGACAAA 
TCAAAGTCTG TGAAGACTAA TTTGATGCAG CTGTTTGAAG AGTCTGGGAA TACAGATATA 
GAAGGAATCG ACACAACTAA TGCATGCTAT GGAGGCACAG CTGCTGTCTT CAATGCTGTT 
AACTGGATTG AGTCCAGCTC TTGGGATGGG CTTCGTGGGA CACATATGCA ACATGCCTAT 
GATTTTTACA AGCCTGATAT GCTATCTGAA TATCCTATAG TAGATGGAAA ACTCTCCATA 
CAGTGCTACC TCAGTGCATT AGACCGCTGC TATTCTGTCT ACTGCAAAAA GATCCATGCC 
CAGTGGCAGA AAGAGGGAAA TGATAAAGAT TTTACCTTGA ATGATTTTGG CTTCATGATC 
TTTCACTCAC CATATTGTAA ACTGGTTCAG AAATCTCTAG CTCGGATGTT GCTGAATGAC 
TTCCTTAATG ACCAGAATAG AGATAAAAAT AGTATCTATA GTGGCCTGGA AGCCTTTGGG 
GATGTTAAAT TAGAAGACAC CTACTTTGAT AGAGATGTGG AGAAGGCATT TATGAAGGCT 
AGCTCTGAAC TCTTCAGTCA GAAAACAAAG GCATCTTTAC TTGTATCAAA TCAAAATGGA 
AATATGTACA CATCTTCAGT ATATGGTTCC CTTGCATCTG TTCTAGCACA GTACTCACCT 
CAGCAATTAG CAGGGAAGAG AATTGGAGTG TTTTCTTATG GTTCTGGTTT GGCTGCCACT 
CTGTACTCTC TTAAAGTCAC ACAAGATGCT ACACCGGGGT CTGCTCTTGA TAAAATAACA 
GCAAGTTTAT GTGATCTTAA ATCAAGGCTT GATTCAAGAA CTGGTGTGGC ACCAGATGTC 
TTCGCTGAAA ACATGAAGCT CAGAGAGGAC ACCCATCATT TGGTCAACTA TATTCCCCAG 
GGTTCAATAG ATTCACTCTT TGAAGGAACG TGGTACTTAG TTAGGGTGGA TGAAAAGCAC 
AGAAGAACTT ACGCTCGGCG TCCCACTCCA AATGATGACA CTTTGGATGA AGGAGTAGGA 
CTTGTGCATT CAAACATAGC AACTGAGCAT ATTCCAAGCC CTGCCAAGAA AGTACCAAGA 
CTCCCTGCCA CAGCAGCAGA ACCTGAAGCA GCTGTCATTA GTAATGGGGA ACATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HMGCS1 ( NM_001324223.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001311152.1 Homo sapiens 3-hydroxy-3-methylglutaryl-CoA synthase 1 (HMGCS1), transcript variant 6, mRNA.

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CloneID	OHu39317
Clone ID Related Accession (Same CDS sequence)	NM_001324222.2 , NM_001324224.1 , NM_001324222.1 , NM_001324223.2 , NM_001324224.2 , NM_001324223.1
Accession Version	NM_001324223.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1437bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-27
Organism	Homo sapiens(human)
Product	hydroxymethylglutaryl-CoA synthase, cytoplasmic isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DB074885.1, AK315593.1 and AC114947.2. On May 31, 2019 this sequence version replaced NM_001324223.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.272420.1, SRR1660803.103675.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03267761 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCTGGAT CACTTCCTTT GAATGCAGAA GCTTGCTGGC CAAAAGATGT GGGAATTGTT 
GCCCTTGAGA TCTATTTTCC TTCTCAATAT GTTGATCAAG CAGAGTTGGA AAAATATGAT 
GGTGTAGATG CTGGAAAGTA TACCATTGGC TTGGGCCAGG CCAAGATGGG CTTCTGCACA 
GATAGAGAAG ATATTAACTC TCTTTGCATG ACTGTGGTTC AGAATCTTAT GGAGAGAAAT 
AACCTTTCCT ATGATTGCAT TGGGCGGCTG GAAGTTGGAA CAGAGACAAT CATCGACAAA 
TCAAAGTCTG TGAAGACTAA TTTGATGCAG CTGTTTGAAG AGTCTGGGAA TACAGATATA 
GAAGGAATCG ACACAACTAA TGCATGCTAT GGAGGCACAG CTGCTGTCTT CAATGCTGTT 
AACTGGATTG AGTCCAGCTC TTGGGATGGG CTTCGTGGGA CACATATGCA ACATGCCTAT 
GATTTTTACA AGCCTGATAT GCTATCTGAA TATCCTATAG TAGATGGAAA ACTCTCCATA 
CAGTGCTACC TCAGTGCATT AGACCGCTGC TATTCTGTCT ACTGCAAAAA GATCCATGCC 
CAGTGGCAGA AAGAGGGAAA TGATAAAGAT TTTACCTTGA ATGATTTTGG CTTCATGATC 
TTTCACTCAC CATATTGTAA ACTGGTTCAG AAATCTCTAG CTCGGATGTT GCTGAATGAC 
TTCCTTAATG ACCAGAATAG AGATAAAAAT AGTATCTATA GTGGCCTGGA AGCCTTTGGG 
GATGTTAAAT TAGAAGACAC CTACTTTGAT AGAGATGTGG AGAAGGCATT TATGAAGGCT 
AGCTCTGAAC TCTTCAGTCA GAAAACAAAG GCATCTTTAC TTGTATCAAA TCAAAATGGA 
AATATGTACA CATCTTCAGT ATATGGTTCC CTTGCATCTG TTCTAGCACA GTACTCACCT 
CAGCAATTAG CAGGGAAGAG AATTGGAGTG TTTTCTTATG GTTCTGGTTT GGCTGCCACT 
CTGTACTCTC TTAAAGTCAC ACAAGATGCT ACACCGGGGT CTGCTCTTGA TAAAATAACA 
GCAAGTTTAT GTGATCTTAA ATCAAGGCTT GATTCAAGAA CTGGTGTGGC ACCAGATGTC 
TTCGCTGAAA ACATGAAGCT CAGAGAGGAC ACCCATCATT TGGTCAACTA TATTCCCCAG 
GGTTCAATAG ATTCACTCTT TGAAGGAACG TGGTACTTAG TTAGGGTGGA TGAAAAGCAC 
AGAAGAACTT ACGCTCGGCG TCCCACTCCA AATGATGACA CTTTGGATGA AGGAGTAGGA 
CTTGTGCATT CAAACATAGC AACTGAGCAT ATTCCAAGCC CTGCCAAGAA AGTACCAAGA 
CTCCCTGCCA CAGCAGCAGA ACCTGAAGCA GCTGTCATTA GTAATGGGGA ACATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001311152.1
CDS	68..1504
Translation	MPGSLPLNAEACWPKDVGIVALEIYFPSQYVDQAELEKYDGVDAGKYTIGLGQAKMGFCTDREDINSLCMTVVQNLMERNNLSYDCIGRLEVGTETIIDKSKSVKTNLMQLFEESGNTDIEGIDTTNACYGGTAAVFNAVNWIESSSWDGLRGTHMQHAYDFYKPDMLSEYPIVDGKLSIQCYLSALDRCYSVYCKKIHAQWQKEGNDKDFTLNDFGFMIFHSPYCKLVQKSLARMLLNDFLNDQNRDKNSIYSGLEAFGDVKLEDTYFDRDVEKAFMKASSELFSQKTKASLLVSNQNGNMYTSSVYGSLASVLAQYSPQQLAGKRIGVFSYGSGLAATLYSLKVTQDATPGSALDKITASLCDLKSRLDSRTGVAPDVFAENMKLREDTHHLVNYIPQGSIDSLFEGTWYLVRVDEKHRRTYARRPTPNDDTLDEGVGLVHSNIATEHIPSPAKKVPRLPATAAEPEAAVISNGEH

Target ORF information:

RefSeq Version	NM_001324223.2
Organism	Homo sapiens(human)
Definition	Homo sapiens 3-hydroxy-3-methylglutaryl-CoA synthase 1 (HMGCS1), transcript variant 6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001324223.2

ORF Insert Sequence:

ATGCCTGGAT CACTTCCTTT GAATGCAGAA GCTTGCTGGC CAAAAGATGT GGGAATTGTT 
GCCCTTGAGA TCTATTTTCC TTCTCAATAT GTTGATCAAG CAGAGTTGGA AAAATATGAT 
GGTGTAGATG CTGGAAAGTA TACCATTGGC TTGGGCCAGG CCAAGATGGG CTTCTGCACA 
GATAGAGAAG ATATTAACTC TCTTTGCATG ACTGTGGTTC AGAATCTTAT GGAGAGAAAT 
AACCTTTCCT ATGATTGCAT TGGGCGGCTG GAAGTTGGAA CAGAGACAAT CATCGACAAA 
TCAAAGTCTG TGAAGACTAA TTTGATGCAG CTGTTTGAAG AGTCTGGGAA TACAGATATA 
GAAGGAATCG ACACAACTAA TGCATGCTAT GGAGGCACAG CTGCTGTCTT CAATGCTGTT 
AACTGGATTG AGTCCAGCTC TTGGGATGGG CTTCGTGGGA CACATATGCA ACATGCCTAT 
GATTTTTACA AGCCTGATAT GCTATCTGAA TATCCTATAG TAGATGGAAA ACTCTCCATA 
CAGTGCTACC TCAGTGCATT AGACCGCTGC TATTCTGTCT ACTGCAAAAA GATCCATGCC 
CAGTGGCAGA AAGAGGGAAA TGATAAAGAT TTTACCTTGA ATGATTTTGG CTTCATGATC 
TTTCACTCAC CATATTGTAA ACTGGTTCAG AAATCTCTAG CTCGGATGTT GCTGAATGAC 
TTCCTTAATG ACCAGAATAG AGATAAAAAT AGTATCTATA GTGGCCTGGA AGCCTTTGGG 
GATGTTAAAT TAGAAGACAC CTACTTTGAT AGAGATGTGG AGAAGGCATT TATGAAGGCT 
AGCTCTGAAC TCTTCAGTCA GAAAACAAAG GCATCTTTAC TTGTATCAAA TCAAAATGGA 
AATATGTACA CATCTTCAGT ATATGGTTCC CTTGCATCTG TTCTAGCACA GTACTCACCT 
CAGCAATTAG CAGGGAAGAG AATTGGAGTG TTTTCTTATG GTTCTGGTTT GGCTGCCACT 
CTGTACTCTC TTAAAGTCAC ACAAGATGCT ACACCGGGGT CTGCTCTTGA TAAAATAACA 
GCAAGTTTAT GTGATCTTAA ATCAAGGCTT GATTCAAGAA CTGGTGTGGC ACCAGATGTC 
TTCGCTGAAA ACATGAAGCT CAGAGAGGAC ACCCATCATT TGGTCAACTA TATTCCCCAG 
GGTTCAATAG ATTCACTCTT TGAAGGAACG TGGTACTTAG TTAGGGTGGA TGAAAAGCAC 
AGAAGAACTT ACGCTCGGCG TCCCACTCCA AATGATGACA CTTTGGATGA AGGAGTAGGA 
CTTGTGCATT CAAACATAGC AACTGAGCAT ATTCCAAGCC CTGCCAAGAA AGTACCAAGA 
CTCCCTGCCA CAGCAGCAGA ACCTGAAGCA GCTGTCATTA GTAATGGGGA ACATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HMGCS1 ( NM_001324224.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001311153.1 Homo sapiens 3-hydroxy-3-methylglutaryl-CoA synthase 1 (HMGCS1), transcript variant 7, mRNA.

Price & Availability↑

CloneID	OHu39317
Clone ID Related Accession (Same CDS sequence)	NM_001324222.2 , NM_001324224.1 , NM_001324222.1 , NM_001324223.2 , NM_001324224.2 , NM_001324223.1
Accession Version	NM_001324224.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1437bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-27
Organism	Homo sapiens(human)
Product	hydroxymethylglutaryl-CoA synthase, cytoplasmic isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BJ995153.1, AK315593.1 and AC114947.2. On May 31, 2019 this sequence version replaced NM_001324224.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.92218.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCTGGAT CACTTCCTTT GAATGCAGAA GCTTGCTGGC CAAAAGATGT GGGAATTGTT 
GCCCTTGAGA TCTATTTTCC TTCTCAATAT GTTGATCAAG CAGAGTTGGA AAAATATGAT 
GGTGTAGATG CTGGAAAGTA TACCATTGGC TTGGGCCAGG CCAAGATGGG CTTCTGCACA 
GATAGAGAAG ATATTAACTC TCTTTGCATG ACTGTGGTTC AGAATCTTAT GGAGAGAAAT 
AACCTTTCCT ATGATTGCAT TGGGCGGCTG GAAGTTGGAA CAGAGACAAT CATCGACAAA 
TCAAAGTCTG TGAAGACTAA TTTGATGCAG CTGTTTGAAG AGTCTGGGAA TACAGATATA 
GAAGGAATCG ACACAACTAA TGCATGCTAT GGAGGCACAG CTGCTGTCTT CAATGCTGTT 
AACTGGATTG AGTCCAGCTC TTGGGATGGG CTTCGTGGGA CACATATGCA ACATGCCTAT 
GATTTTTACA AGCCTGATAT GCTATCTGAA TATCCTATAG TAGATGGAAA ACTCTCCATA 
CAGTGCTACC TCAGTGCATT AGACCGCTGC TATTCTGTCT ACTGCAAAAA GATCCATGCC 
CAGTGGCAGA AAGAGGGAAA TGATAAAGAT TTTACCTTGA ATGATTTTGG CTTCATGATC 
TTTCACTCAC CATATTGTAA ACTGGTTCAG AAATCTCTAG CTCGGATGTT GCTGAATGAC 
TTCCTTAATG ACCAGAATAG AGATAAAAAT AGTATCTATA GTGGCCTGGA AGCCTTTGGG 
GATGTTAAAT TAGAAGACAC CTACTTTGAT AGAGATGTGG AGAAGGCATT TATGAAGGCT 
AGCTCTGAAC TCTTCAGTCA GAAAACAAAG GCATCTTTAC TTGTATCAAA TCAAAATGGA 
AATATGTACA CATCTTCAGT ATATGGTTCC CTTGCATCTG TTCTAGCACA GTACTCACCT 
CAGCAATTAG CAGGGAAGAG AATTGGAGTG TTTTCTTATG GTTCTGGTTT GGCTGCCACT 
CTGTACTCTC TTAAAGTCAC ACAAGATGCT ACACCGGGGT CTGCTCTTGA TAAAATAACA 
GCAAGTTTAT GTGATCTTAA ATCAAGGCTT GATTCAAGAA CTGGTGTGGC ACCAGATGTC 
TTCGCTGAAA ACATGAAGCT CAGAGAGGAC ACCCATCATT TGGTCAACTA TATTCCCCAG 
GGTTCAATAG ATTCACTCTT TGAAGGAACG TGGTACTTAG TTAGGGTGGA TGAAAAGCAC 
AGAAGAACTT ACGCTCGGCG TCCCACTCCA AATGATGACA CTTTGGATGA AGGAGTAGGA 
CTTGTGCATT CAAACATAGC AACTGAGCAT ATTCCAAGCC CTGCCAAGAA AGTACCAAGA 
CTCCCTGCCA CAGCAGCAGA ACCTGAAGCA GCTGTCATTA GTAATGGGGA ACATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001311153.1
CDS	147..1583
Translation	MPGSLPLNAEACWPKDVGIVALEIYFPSQYVDQAELEKYDGVDAGKYTIGLGQAKMGFCTDREDINSLCMTVVQNLMERNNLSYDCIGRLEVGTETIIDKSKSVKTNLMQLFEESGNTDIEGIDTTNACYGGTAAVFNAVNWIESSSWDGLRGTHMQHAYDFYKPDMLSEYPIVDGKLSIQCYLSALDRCYSVYCKKIHAQWQKEGNDKDFTLNDFGFMIFHSPYCKLVQKSLARMLLNDFLNDQNRDKNSIYSGLEAFGDVKLEDTYFDRDVEKAFMKASSELFSQKTKASLLVSNQNGNMYTSSVYGSLASVLAQYSPQQLAGKRIGVFSYGSGLAATLYSLKVTQDATPGSALDKITASLCDLKSRLDSRTGVAPDVFAENMKLREDTHHLVNYIPQGSIDSLFEGTWYLVRVDEKHRRTYARRPTPNDDTLDEGVGLVHSNIATEHIPSPAKKVPRLPATAAEPEAAVISNGEH

Target ORF information:

RefSeq Version	NM_001324224.2
Organism	Homo sapiens(human)
Definition	Homo sapiens 3-hydroxy-3-methylglutaryl-CoA synthase 1 (HMGCS1), transcript variant 7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001324224.2

ORF Insert Sequence:

ATGCCTGGAT CACTTCCTTT GAATGCAGAA GCTTGCTGGC CAAAAGATGT GGGAATTGTT 
GCCCTTGAGA TCTATTTTCC TTCTCAATAT GTTGATCAAG CAGAGTTGGA AAAATATGAT 
GGTGTAGATG CTGGAAAGTA TACCATTGGC TTGGGCCAGG CCAAGATGGG CTTCTGCACA 
GATAGAGAAG ATATTAACTC TCTTTGCATG ACTGTGGTTC AGAATCTTAT GGAGAGAAAT 
AACCTTTCCT ATGATTGCAT TGGGCGGCTG GAAGTTGGAA CAGAGACAAT CATCGACAAA 
TCAAAGTCTG TGAAGACTAA TTTGATGCAG CTGTTTGAAG AGTCTGGGAA TACAGATATA 
GAAGGAATCG ACACAACTAA TGCATGCTAT GGAGGCACAG CTGCTGTCTT CAATGCTGTT 
AACTGGATTG AGTCCAGCTC TTGGGATGGG CTTCGTGGGA CACATATGCA ACATGCCTAT 
GATTTTTACA AGCCTGATAT GCTATCTGAA TATCCTATAG TAGATGGAAA ACTCTCCATA 
CAGTGCTACC TCAGTGCATT AGACCGCTGC TATTCTGTCT ACTGCAAAAA GATCCATGCC 
CAGTGGCAGA AAGAGGGAAA TGATAAAGAT TTTACCTTGA ATGATTTTGG CTTCATGATC 
TTTCACTCAC CATATTGTAA ACTGGTTCAG AAATCTCTAG CTCGGATGTT GCTGAATGAC 
TTCCTTAATG ACCAGAATAG AGATAAAAAT AGTATCTATA GTGGCCTGGA AGCCTTTGGG 
GATGTTAAAT TAGAAGACAC CTACTTTGAT AGAGATGTGG AGAAGGCATT TATGAAGGCT 
AGCTCTGAAC TCTTCAGTCA GAAAACAAAG GCATCTTTAC TTGTATCAAA TCAAAATGGA 
AATATGTACA CATCTTCAGT ATATGGTTCC CTTGCATCTG TTCTAGCACA GTACTCACCT 
CAGCAATTAG CAGGGAAGAG AATTGGAGTG TTTTCTTATG GTTCTGGTTT GGCTGCCACT 
CTGTACTCTC TTAAAGTCAC ACAAGATGCT ACACCGGGGT CTGCTCTTGA TAAAATAACA 
GCAAGTTTAT GTGATCTTAA ATCAAGGCTT GATTCAAGAA CTGGTGTGGC ACCAGATGTC 
TTCGCTGAAA ACATGAAGCT CAGAGAGGAC ACCCATCATT TGGTCAACTA TATTCCCCAG 
GGTTCAATAG ATTCACTCTT TGAAGGAACG TGGTACTTAG TTAGGGTGGA TGAAAAGCAC 
AGAAGAACTT ACGCTCGGCG TCCCACTCCA AATGATGACA CTTTGGATGA AGGAGTAGGA 
CTTGTGCATT CAAACATAGC AACTGAGCAT ATTCCAAGCC CTGCCAAGAA AGTACCAAGA 
CTCCCTGCCA CAGCAGCAGA ACCTGAAGCA GCTGTCATTA GTAATGGGGA ACATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HMGCS1 ( NM_001324223.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001311152.1 Homo sapiens 3-hydroxy-3-methylglutaryl-CoA synthase 1 (HMGCS1), transcript variant 6, mRNA.

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CloneID	OHu39317
Clone ID Related Accession (Same CDS sequence)	NM_001324222.2 , NM_001324224.1 , NM_001324222.1 , NM_001324223.2 , NM_001324224.2 , NM_001324223.1
Accession Version	NM_001324223.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1437bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-24
Organism	Homo sapiens(human)
Product	hydroxymethylglutaryl-CoA synthase, cytoplasmic isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DB074885.1, AK315593.1 and AC114947.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.284767.1, SRR1803617.187120.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03267761 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCTGGAT CACTTCCTTT GAATGCAGAA GCTTGCTGGC CAAAAGATGT GGGAATTGTT 
GCCCTTGAGA TCTATTTTCC TTCTCAATAT GTTGATCAAG CAGAGTTGGA AAAATATGAT 
GGTGTAGATG CTGGAAAGTA TACCATTGGC TTGGGCCAGG CCAAGATGGG CTTCTGCACA 
GATAGAGAAG ATATTAACTC TCTTTGCATG ACTGTGGTTC AGAATCTTAT GGAGAGAAAT 
AACCTTTCCT ATGATTGCAT TGGGCGGCTG GAAGTTGGAA CAGAGACAAT CATCGACAAA 
TCAAAGTCTG TGAAGACTAA TTTGATGCAG CTGTTTGAAG AGTCTGGGAA TACAGATATA 
GAAGGAATCG ACACAACTAA TGCATGCTAT GGAGGCACAG CTGCTGTCTT CAATGCTGTT 
AACTGGATTG AGTCCAGCTC TTGGGATGGG CTTCGTGGGA CACATATGCA ACATGCCTAT 
GATTTTTACA AGCCTGATAT GCTATCTGAA TATCCTATAG TAGATGGAAA ACTCTCCATA 
CAGTGCTACC TCAGTGCATT AGACCGCTGC TATTCTGTCT ACTGCAAAAA GATCCATGCC 
CAGTGGCAGA AAGAGGGAAA TGATAAAGAT TTTACCTTGA ATGATTTTGG CTTCATGATC 
TTTCACTCAC CATATTGTAA ACTGGTTCAG AAATCTCTAG CTCGGATGTT GCTGAATGAC 
TTCCTTAATG ACCAGAATAG AGATAAAAAT AGTATCTATA GTGGCCTGGA AGCCTTTGGG 
GATGTTAAAT TAGAAGACAC CTACTTTGAT AGAGATGTGG AGAAGGCATT TATGAAGGCT 
AGCTCTGAAC TCTTCAGTCA GAAAACAAAG GCATCTTTAC TTGTATCAAA TCAAAATGGA 
AATATGTACA CATCTTCAGT ATATGGTTCC CTTGCATCTG TTCTAGCACA GTACTCACCT 
CAGCAATTAG CAGGGAAGAG AATTGGAGTG TTTTCTTATG GTTCTGGTTT GGCTGCCACT 
CTGTACTCTC TTAAAGTCAC ACAAGATGCT ACACCGGGGT CTGCTCTTGA TAAAATAACA 
GCAAGTTTAT GTGATCTTAA ATCAAGGCTT GATTCAAGAA CTGGTGTGGC ACCAGATGTC 
TTCGCTGAAA ACATGAAGCT CAGAGAGGAC ACCCATCATT TGGTCAACTA TATTCCCCAG 
GGTTCAATAG ATTCACTCTT TGAAGGAACG TGGTACTTAG TTAGGGTGGA TGAAAAGCAC 
AGAAGAACTT ACGCTCGGCG TCCCACTCCA AATGATGACA CTTTGGATGA AGGAGTAGGA 
CTTGTGCATT CAAACATAGC AACTGAGCAT ATTCCAAGCC CTGCCAAGAA AGTACCAAGA 
CTCCCTGCCA CAGCAGCAGA ACCTGAAGCA GCTGTCATTA GTAATGGGGA ACATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001311152.1
CDS	168..1604
Translation	MPGSLPLNAEACWPKDVGIVALEIYFPSQYVDQAELEKYDGVDAGKYTIGLGQAKMGFCTDREDINSLCMTVVQNLMERNNLSYDCIGRLEVGTETIIDKSKSVKTNLMQLFEESGNTDIEGIDTTNACYGGTAAVFNAVNWIESSSWDGLRGTHMQHAYDFYKPDMLSEYPIVDGKLSIQCYLSALDRCYSVYCKKIHAQWQKEGNDKDFTLNDFGFMIFHSPYCKLVQKSLARMLLNDFLNDQNRDKNSIYSGLEAFGDVKLEDTYFDRDVEKAFMKASSELFSQKTKASLLVSNQNGNMYTSSVYGSLASVLAQYSPQQLAGKRIGVFSYGSGLAATLYSLKVTQDATPGSALDKITASLCDLKSRLDSRTGVAPDVFAENMKLREDTHHLVNYIPQGSIDSLFEGTWYLVRVDEKHRRTYARRPTPNDDTLDEGVGLVHSNIATEHIPSPAKKVPRLPATAAEPEAAVISNGEH

Target ORF information:

RefSeq Version	NM_001324223.1
Organism	Homo sapiens(human)
Definition	Homo sapiens 3-hydroxy-3-methylglutaryl-CoA synthase 1 (HMGCS1), transcript variant 6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001324223.1

ORF Insert Sequence:

ATGCCTGGAT CACTTCCTTT GAATGCAGAA GCTTGCTGGC CAAAAGATGT GGGAATTGTT 
GCCCTTGAGA TCTATTTTCC TTCTCAATAT GTTGATCAAG CAGAGTTGGA AAAATATGAT 
GGTGTAGATG CTGGAAAGTA TACCATTGGC TTGGGCCAGG CCAAGATGGG CTTCTGCACA 
GATAGAGAAG ATATTAACTC TCTTTGCATG ACTGTGGTTC AGAATCTTAT GGAGAGAAAT 
AACCTTTCCT ATGATTGCAT TGGGCGGCTG GAAGTTGGAA CAGAGACAAT CATCGACAAA 
TCAAAGTCTG TGAAGACTAA TTTGATGCAG CTGTTTGAAG AGTCTGGGAA TACAGATATA 
GAAGGAATCG ACACAACTAA TGCATGCTAT GGAGGCACAG CTGCTGTCTT CAATGCTGTT 
AACTGGATTG AGTCCAGCTC TTGGGATGGG CTTCGTGGGA CACATATGCA ACATGCCTAT 
GATTTTTACA AGCCTGATAT GCTATCTGAA TATCCTATAG TAGATGGAAA ACTCTCCATA 
CAGTGCTACC TCAGTGCATT AGACCGCTGC TATTCTGTCT ACTGCAAAAA GATCCATGCC 
CAGTGGCAGA AAGAGGGAAA TGATAAAGAT TTTACCTTGA ATGATTTTGG CTTCATGATC 
TTTCACTCAC CATATTGTAA ACTGGTTCAG AAATCTCTAG CTCGGATGTT GCTGAATGAC 
TTCCTTAATG ACCAGAATAG AGATAAAAAT AGTATCTATA GTGGCCTGGA AGCCTTTGGG 
GATGTTAAAT TAGAAGACAC CTACTTTGAT AGAGATGTGG AGAAGGCATT TATGAAGGCT 
AGCTCTGAAC TCTTCAGTCA GAAAACAAAG GCATCTTTAC TTGTATCAAA TCAAAATGGA 
AATATGTACA CATCTTCAGT ATATGGTTCC CTTGCATCTG TTCTAGCACA GTACTCACCT 
CAGCAATTAG CAGGGAAGAG AATTGGAGTG TTTTCTTATG GTTCTGGTTT GGCTGCCACT 
CTGTACTCTC TTAAAGTCAC ACAAGATGCT ACACCGGGGT CTGCTCTTGA TAAAATAACA 
GCAAGTTTAT GTGATCTTAA ATCAAGGCTT GATTCAAGAA CTGGTGTGGC ACCAGATGTC 
TTCGCTGAAA ACATGAAGCT CAGAGAGGAC ACCCATCATT TGGTCAACTA TATTCCCCAG 
GGTTCAATAG ATTCACTCTT TGAAGGAACG TGGTACTTAG TTAGGGTGGA TGAAAAGCAC 
AGAAGAACTT ACGCTCGGCG TCCCACTCCA AATGATGACA CTTTGGATGA AGGAGTAGGA 
CTTGTGCATT CAAACATAGC AACTGAGCAT ATTCCAAGCC CTGCCAAGAA AGTACCAAGA 
CTCCCTGCCA CAGCAGCAGA ACCTGAAGCA GCTGTCATTA GTAATGGGGA ACATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HMGCS1 ( XM_011514036.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011512338.1 Homo sapiens 3-hydroxy-3-methylglutaryl-CoA synthase 1 (HMGCS1), transcript variant X2, mRNA.

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CloneID	OHu58268
Clone ID Related Accession (Same CDS sequence)	XM_011514036.3 , XM_024446038.1 , XM_011514036.2
Accession Version	XM_011514036.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 972bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	hydroxymethylglutaryl-CoA synthase, cytoplasmic isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011514036.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAACATG CCTATGATTT TTACAAGCCT GATATGCTAT CTGAATATCC TATAGTAGAT 
GGAAAACTCT CCATACAGTG CTACCTCAGT GCATTAGACC GCTGCTATTC TGTCTACTGC 
AAAAAGATCC ATGCCCAGTG GCAGAAAGAG GGAAATGATA AAGATTTTAC CTTGAATGAT 
TTTGGCTTCA TGATCTTTCA CTCACCATAT TGTAAACTGG TTCAGAAATC TCTAGCTCGG 
ATGTTGCTGA ATGACTTCCT TAATGACCAG AATAGAGATA AAAATAGTAT CTATAGTGGC 
CTGGAAGCCT TTGGGGATGT TAAATTAGAA GACACCTACT TTGATAGAGA TGTGGAGAAG 
GCATTTATGA AGGCTAGCTC TGAACTCTTC AGTCAGAAAA CAAAGGCATC TTTACTTGTA 
TCAAATCAAA ATGGAAATAT GTACACATCT TCAGTATATG GTTCCCTTGC ATCTGTTCTA 
GCACAGTACT CACCTCAGCA ATTAGCAGGG AAGAGAATTG GAGTGTTTTC TTATGGTTCT 
GGTTTGGCTG CCACTCTGTA CTCTCTTAAA GTCACACAAG ATGCTACACC GGGGTCTGCT 
CTTGATAAAA TAACAGCAAG TTTATGTGAT CTTAAATCAA GGCTTGATTC AAGAACTGGT 
GTGGCACCAG ATGTCTTCGC TGAAAACATG AAGCTCAGAG AGGACACCCA TCATTTGGTC 
AACTATATTC CCCAGGGTTC AATAGATTCA CTCTTTGAAG GAACGTGGTA CTTAGTTAGG 
GTGGATGAAA AGCACAGAAG AACTTACGCT CGGCGTCCCA CTCCAAATGA TGACACTTTG 
GATGAAGGAG TAGGACTTGT GCATTCAAAC ATAGCAACTG AGCATATTCC AAGCCCTGCC 
AAGAAAGTAC CAAGACTCCC TGCCACAGCA GCAGAACCTG AAGCAGCTGT CATTAGTAAT 
GGGGAACATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011512338.1
CDS	1587..2558
Translation	MQHAYDFYKPDMLSEYPIVDGKLSIQCYLSALDRCYSVYCKKIHAQWQKEGNDKDFTLNDFGFMIFHSPYCKLVQKSLARMLLNDFLNDQNRDKNSIYSGLEAFGDVKLEDTYFDRDVEKAFMKASSELFSQKTKASLLVSNQNGNMYTSSVYGSLASVLAQYSPQQLAGKRIGVFSYGSGLAATLYSLKVTQDATPGSALDKITASLCDLKSRLDSRTGVAPDVFAENMKLREDTHHLVNYIPQGSIDSLFEGTWYLVRVDEKHRRTYARRPTPNDDTLDEGVGLVHSNIATEHIPSPAKKVPRLPATAAEPEAAVISNGEH

Target ORF information:

RefSeq Version	XM_011514036.3
Organism	Homo sapiens(human)
Definition	Homo sapiens 3-hydroxy-3-methylglutaryl-CoA synthase 1 (HMGCS1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011514036.3

ORF Insert Sequence:

ATGCAACATG CCTATGATTT TTACAAGCCT GATATGCTAT CTGAATATCC TATAGTAGAT 
GGAAAACTCT CCATACAGTG CTACCTCAGT GCATTAGACC GCTGCTATTC TGTCTACTGC 
AAAAAGATCC ATGCCCAGTG GCAGAAAGAG GGAAATGATA AAGATTTTAC CTTGAATGAT 
TTTGGCTTCA TGATCTTTCA CTCACCATAT TGTAAACTGG TTCAGAAATC TCTAGCTCGG 
ATGTTGCTGA ATGACTTCCT TAATGACCAG AATAGAGATA AAAATAGTAT CTATAGTGGC 
CTGGAAGCCT TTGGGGATGT TAAATTAGAA GACACCTACT TTGATAGAGA TGTGGAGAAG 
GCATTTATGA AGGCTAGCTC TGAACTCTTC AGTCAGAAAA CAAAGGCATC TTTACTTGTA 
TCAAATCAAA ATGGAAATAT GTACACATCT TCAGTATATG GTTCCCTTGC ATCTGTTCTA 
GCACAGTACT CACCTCAGCA ATTAGCAGGG AAGAGAATTG GAGTGTTTTC TTATGGTTCT 
GGTTTGGCTG CCACTCTGTA CTCTCTTAAA GTCACACAAG ATGCTACACC GGGGTCTGCT 
CTTGATAAAA TAACAGCAAG TTTATGTGAT CTTAAATCAA GGCTTGATTC AAGAACTGGT 
GTGGCACCAG ATGTCTTCGC TGAAAACATG AAGCTCAGAG AGGACACCCA TCATTTGGTC 
AACTATATTC CCCAGGGTTC AATAGATTCA CTCTTTGAAG GAACGTGGTA CTTAGTTAGG 
GTGGATGAAA AGCACAGAAG AACTTACGCT CGGCGTCCCA CTCCAAATGA TGACACTTTG 
GATGAAGGAG TAGGACTTGT GCATTCAAAC ATAGCAACTG AGCATATTCC AAGCCCTGCC 
AAGAAAGTAC CAAGACTCCC TGCCACAGCA GCAGAACCTG AAGCAGCTGT CATTAGTAAT 
GGGGAACATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HMGCS1 ( XM_024446038.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024301806.1 Homo sapiens 3-hydroxy-3-methylglutaryl-CoA synthase 1 (HMGCS1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu58268
Clone ID Related Accession (Same CDS sequence)	XM_011514036.3 , XM_024446038.1 , XM_011514036.2
Accession Version	XM_024446038.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 972bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	hydroxymethylglutaryl-CoA synthase, cytoplasmic isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAACATG CCTATGATTT TTACAAGCCT GATATGCTAT CTGAATATCC TATAGTAGAT 
GGAAAACTCT CCATACAGTG CTACCTCAGT GCATTAGACC GCTGCTATTC TGTCTACTGC 
AAAAAGATCC ATGCCCAGTG GCAGAAAGAG GGAAATGATA AAGATTTTAC CTTGAATGAT 
TTTGGCTTCA TGATCTTTCA CTCACCATAT TGTAAACTGG TTCAGAAATC TCTAGCTCGG 
ATGTTGCTGA ATGACTTCCT TAATGACCAG AATAGAGATA AAAATAGTAT CTATAGTGGC 
CTGGAAGCCT TTGGGGATGT TAAATTAGAA GACACCTACT TTGATAGAGA TGTGGAGAAG 
GCATTTATGA AGGCTAGCTC TGAACTCTTC AGTCAGAAAA CAAAGGCATC TTTACTTGTA 
TCAAATCAAA ATGGAAATAT GTACACATCT TCAGTATATG GTTCCCTTGC ATCTGTTCTA 
GCACAGTACT CACCTCAGCA ATTAGCAGGG AAGAGAATTG GAGTGTTTTC TTATGGTTCT 
GGTTTGGCTG CCACTCTGTA CTCTCTTAAA GTCACACAAG ATGCTACACC GGGGTCTGCT 
CTTGATAAAA TAACAGCAAG TTTATGTGAT CTTAAATCAA GGCTTGATTC AAGAACTGGT 
GTGGCACCAG ATGTCTTCGC TGAAAACATG AAGCTCAGAG AGGACACCCA TCATTTGGTC 
AACTATATTC CCCAGGGTTC AATAGATTCA CTCTTTGAAG GAACGTGGTA CTTAGTTAGG 
GTGGATGAAA AGCACAGAAG AACTTACGCT CGGCGTCCCA CTCCAAATGA TGACACTTTG 
GATGAAGGAG TAGGACTTGT GCATTCAAAC ATAGCAACTG AGCATATTCC AAGCCCTGCC 
AAGAAAGTAC CAAGACTCCC TGCCACAGCA GCAGAACCTG AAGCAGCTGT CATTAGTAAT 
GGGGAACATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024301806.1
CDS	1636..2607
Translation	MQHAYDFYKPDMLSEYPIVDGKLSIQCYLSALDRCYSVYCKKIHAQWQKEGNDKDFTLNDFGFMIFHSPYCKLVQKSLARMLLNDFLNDQNRDKNSIYSGLEAFGDVKLEDTYFDRDVEKAFMKASSELFSQKTKASLLVSNQNGNMYTSSVYGSLASVLAQYSPQQLAGKRIGVFSYGSGLAATLYSLKVTQDATPGSALDKITASLCDLKSRLDSRTGVAPDVFAENMKLREDTHHLVNYIPQGSIDSLFEGTWYLVRVDEKHRRTYARRPTPNDDTLDEGVGLVHSNIATEHIPSPAKKVPRLPATAAEPEAAVISNGEH

Target ORF information:

RefSeq Version	XM_024446038.1
Organism	Homo sapiens(human)
Definition	Homo sapiens 3-hydroxy-3-methylglutaryl-CoA synthase 1 (HMGCS1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024446038.1

ORF Insert Sequence:

ATGCAACATG CCTATGATTT TTACAAGCCT GATATGCTAT CTGAATATCC TATAGTAGAT 
GGAAAACTCT CCATACAGTG CTACCTCAGT GCATTAGACC GCTGCTATTC TGTCTACTGC 
AAAAAGATCC ATGCCCAGTG GCAGAAAGAG GGAAATGATA AAGATTTTAC CTTGAATGAT 
TTTGGCTTCA TGATCTTTCA CTCACCATAT TGTAAACTGG TTCAGAAATC TCTAGCTCGG 
ATGTTGCTGA ATGACTTCCT TAATGACCAG AATAGAGATA AAAATAGTAT CTATAGTGGC 
CTGGAAGCCT TTGGGGATGT TAAATTAGAA GACACCTACT TTGATAGAGA TGTGGAGAAG 
GCATTTATGA AGGCTAGCTC TGAACTCTTC AGTCAGAAAA CAAAGGCATC TTTACTTGTA 
TCAAATCAAA ATGGAAATAT GTACACATCT TCAGTATATG GTTCCCTTGC ATCTGTTCTA 
GCACAGTACT CACCTCAGCA ATTAGCAGGG AAGAGAATTG GAGTGTTTTC TTATGGTTCT 
GGTTTGGCTG CCACTCTGTA CTCTCTTAAA GTCACACAAG ATGCTACACC GGGGTCTGCT 
CTTGATAAAA TAACAGCAAG TTTATGTGAT CTTAAATCAA GGCTTGATTC AAGAACTGGT 
GTGGCACCAG ATGTCTTCGC TGAAAACATG AAGCTCAGAG AGGACACCCA TCATTTGGTC 
AACTATATTC CCCAGGGTTC AATAGATTCA CTCTTTGAAG GAACGTGGTA CTTAGTTAGG 
GTGGATGAAA AGCACAGAAG AACTTACGCT CGGCGTCCCA CTCCAAATGA TGACACTTTG 
GATGAAGGAG TAGGACTTGT GCATTCAAAC ATAGCAACTG AGCATATTCC AAGCCCTGCC 
AAGAAAGTAC CAAGACTCCC TGCCACAGCA GCAGAACCTG AAGCAGCTGT CATTAGTAAT 
GGGGAACATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HMGCS1 ( XM_011514036.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011512338.1 Homo sapiens 3-hydroxy-3-methylglutaryl-CoA synthase 1 (HMGCS1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu58268
Clone ID Related Accession (Same CDS sequence)	XM_011514036.3 , XM_024446038.1 , XM_011514036.2
Accession Version	XM_011514036.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 972bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	hydroxymethylglutaryl-CoA synthase, cytoplasmic isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011514036.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAACATG CCTATGATTT TTACAAGCCT GATATGCTAT CTGAATATCC TATAGTAGAT 
GGAAAACTCT CCATACAGTG CTACCTCAGT GCATTAGACC GCTGCTATTC TGTCTACTGC 
AAAAAGATCC ATGCCCAGTG GCAGAAAGAG GGAAATGATA AAGATTTTAC CTTGAATGAT 
TTTGGCTTCA TGATCTTTCA CTCACCATAT TGTAAACTGG TTCAGAAATC TCTAGCTCGG 
ATGTTGCTGA ATGACTTCCT TAATGACCAG AATAGAGATA AAAATAGTAT CTATAGTGGC 
CTGGAAGCCT TTGGGGATGT TAAATTAGAA GACACCTACT TTGATAGAGA TGTGGAGAAG 
GCATTTATGA AGGCTAGCTC TGAACTCTTC AGTCAGAAAA CAAAGGCATC TTTACTTGTA 
TCAAATCAAA ATGGAAATAT GTACACATCT TCAGTATATG GTTCCCTTGC ATCTGTTCTA 
GCACAGTACT CACCTCAGCA ATTAGCAGGG AAGAGAATTG GAGTGTTTTC TTATGGTTCT 
GGTTTGGCTG CCACTCTGTA CTCTCTTAAA GTCACACAAG ATGCTACACC GGGGTCTGCT 
CTTGATAAAA TAACAGCAAG TTTATGTGAT CTTAAATCAA GGCTTGATTC AAGAACTGGT 
GTGGCACCAG ATGTCTTCGC TGAAAACATG AAGCTCAGAG AGGACACCCA TCATTTGGTC 
AACTATATTC CCCAGGGTTC AATAGATTCA CTCTTTGAAG GAACGTGGTA CTTAGTTAGG 
GTGGATGAAA AGCACAGAAG AACTTACGCT CGGCGTCCCA CTCCAAATGA TGACACTTTG 
GATGAAGGAG TAGGACTTGT GCATTCAAAC ATAGCAACTG AGCATATTCC AAGCCCTGCC 
AAGAAAGTAC CAAGACTCCC TGCCACAGCA GCAGAACCTG AAGCAGCTGT CATTAGTAAT 
GGGGAACATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011512338.1
CDS	624..1595
Translation	MQHAYDFYKPDMLSEYPIVDGKLSIQCYLSALDRCYSVYCKKIHAQWQKEGNDKDFTLNDFGFMIFHSPYCKLVQKSLARMLLNDFLNDQNRDKNSIYSGLEAFGDVKLEDTYFDRDVEKAFMKASSELFSQKTKASLLVSNQNGNMYTSSVYGSLASVLAQYSPQQLAGKRIGVFSYGSGLAATLYSLKVTQDATPGSALDKITASLCDLKSRLDSRTGVAPDVFAENMKLREDTHHLVNYIPQGSIDSLFEGTWYLVRVDEKHRRTYARRPTPNDDTLDEGVGLVHSNIATEHIPSPAKKVPRLPATAAEPEAAVISNGEH

Target ORF information:

RefSeq Version	XM_011514036.2
Organism	Homo sapiens(human)
Definition	Homo sapiens 3-hydroxy-3-methylglutaryl-CoA synthase 1 (HMGCS1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011514036.2

ORF Insert Sequence:

ATGCAACATG CCTATGATTT TTACAAGCCT GATATGCTAT CTGAATATCC TATAGTAGAT 
GGAAAACTCT CCATACAGTG CTACCTCAGT GCATTAGACC GCTGCTATTC TGTCTACTGC 
AAAAAGATCC ATGCCCAGTG GCAGAAAGAG GGAAATGATA AAGATTTTAC CTTGAATGAT 
TTTGGCTTCA TGATCTTTCA CTCACCATAT TGTAAACTGG TTCAGAAATC TCTAGCTCGG 
ATGTTGCTGA ATGACTTCCT TAATGACCAG AATAGAGATA AAAATAGTAT CTATAGTGGC 
CTGGAAGCCT TTGGGGATGT TAAATTAGAA GACACCTACT TTGATAGAGA TGTGGAGAAG 
GCATTTATGA AGGCTAGCTC TGAACTCTTC AGTCAGAAAA CAAAGGCATC TTTACTTGTA 
TCAAATCAAA ATGGAAATAT GTACACATCT TCAGTATATG GTTCCCTTGC ATCTGTTCTA 
GCACAGTACT CACCTCAGCA ATTAGCAGGG AAGAGAATTG GAGTGTTTTC TTATGGTTCT 
GGTTTGGCTG CCACTCTGTA CTCTCTTAAA GTCACACAAG ATGCTACACC GGGGTCTGCT 
CTTGATAAAA TAACAGCAAG TTTATGTGAT CTTAAATCAA GGCTTGATTC AAGAACTGGT 
GTGGCACCAG ATGTCTTCGC TGAAAACATG AAGCTCAGAG AGGACACCCA TCATTTGGTC 
AACTATATTC CCCAGGGTTC AATAGATTCA CTCTTTGAAG GAACGTGGTA CTTAGTTAGG 
GTGGATGAAA AGCACAGAAG AACTTACGCT CGGCGTCCCA CTCCAAATGA TGACACTTTG 
GATGAAGGAG TAGGACTTGT GCATTCAAAC ATAGCAACTG AGCATATTCC AAGCCCTGCC 
AAGAAAGTAC CAAGACTCCC TGCCACAGCA GCAGAACCTG AAGCAGCTGT CATTAGTAAT 
GGGGAACATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

HMG-CoA synthase 1 is a synthetic lethal partner of BRAF
The Journal of biological chemistry292(24)10142-10152(2017 06)
Zhao L,Fan J,Xia S,Pan Y,Liu S,Qian G,Qian Z,Kang HB,Arbiser JL,Pollack BP,Kudchadkar RR,Lawson DH,Rossi M,Abdel-Wahab O,Merghoub T,Khoury HJ,Khuri FR,Boise LH,Lonial S,Chen F,Chen J,Lin R

Crystal structures of human HMG-CoA synthase isoforms provide insights into inherited ketogenesis disorders and inhibitor design.
Journal of molecular biology398(4)497-506(2010 May)
Shafqat N,Turnbull A,Zschocke J,Oppermann U,Yue WW

Multiple genetic variants along candidate pathways influence plasma high-density lipoprotein cholesterol concentrations.
Journal of lipid research49(12)2582-9(2008 Dec)
Lu Y,Doll? ME,Imholz S,van 't Slot R,Verschuren WM,Wijmenga C,Feskens EJ,Boer JM

Transcriptional regulation of HMG-CoA synthase and HMG-CoA reductase genes by human ACBP.
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology22(5-6)515-24(2008)
Vock C,D?ring F,Nitz I

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HMGCS1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

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