LIMA1 cDNA ORF clone, Canis lupus familiaris(dog)

The following LIMA1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LIMA1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe27009 XM_005636849.3
Latest version!
Canis lupus familiaris LIM domain and actin binding 1 (LIMA1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OCe27009 XM_022410909.1
Latest version!
Canis lupus familiaris LIM domain and actin binding 1 (LIMA1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OCe27010 XM_005636850.3
Latest version!
Canis lupus familiaris LIM domain and actin binding 1 (LIMA1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OCe27011 XM_022410910.1
Latest version!
Canis lupus familiaris LIM domain and actin binding 1 (LIMA1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OCe27011 XM_005636851.3
Latest version!
Canis lupus familiaris LIM domain and actin binding 1 (LIMA1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OCe27012 XM_534804.6
Latest version!
Canis lupus familiaris LIM domain and actin binding 1 (LIMA1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
View Old Accession Versions >>
OCe27011 XM_005636851.2 Canis lupus familiaris LIM domain and actin binding 1 (LIMA1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OCe27010 XM_005636850.2 Canis lupus familiaris LIM domain and actin binding 1 (LIMA1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OCe27009 XM_005636849.2 Canis lupus familiaris LIM domain and actin binding 1 (LIMA1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OCe27012 XM_534804.5 Canis lupus familiaris LIM domain and actin binding 1 (LIMA1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe27009
    Clone ID Related Accession (Same CDS sequence) XM_022410909.1 , XM_005636849.3 , XM_005636849.2
    Accession Version XM_022410909.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2292bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product LIM domain and actin-binding protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006609.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGAATCGA CTCCATTTAA CAGACGGCAA TGGACTTCAG TATCATTGAG GGTAACAGCC 
    AGAGAACTTT CTCTTGTCAA CAAGAATAAG TCATCGGCTA TTGTGGAAAT ATTCTCCAAG
    TACCAGAAAG CAGCTGAAGA AGCAAACATG GAAAAGAGGA GAAGTAACAC TGAAAATCTC
    CCCCAGCACT GCAGGAGGGG GATCCTGACG GTGTTAAAGA AGAAGTGGGA GAACCCAGCA
    CTGGGAACAG AGCCTCACCC AGACTCCCTG CGAAACGGCA GCACTGAGGT CAGACCCGGA
    GGCGACCATC CTCCGGGTGA AGTGGCAAGA AGCTCTGCCT CTGAGGCCGA AGCTGACCAG
    GAAGAAAGAG CCCACCCCAG ACCTAGGCAC GGATCACCTC CTGAAGCCCT AATTCAGTGT
    CCGTATCCCC GCACTGAGGG CAGTGAGCAT CTTAAAGACC CCTCGAAAGA AAGTAAAAAA
    ATGGAAACGT GTGTAGGAGA ACCCAGGCAT GAAGTAGGAA AACCTGAAAT CAGTGAAAAC
    ACAGAAGGCG CAAACAAAAT AGAGAAATAC AATGTTCCGC TGAACAGGCT TAAGATGATG
    TTTGAGAAAG GTGAACCGAC TCAAACCAAG CAGATTCTTC GGACCCAGAG CCGTAGTGCA
    GGTGGGAGGA GGATCTCAGA AAACAGCTAT TCTCTGGATG ATTTGGAAAT AGGCGCAGGT
    CAGGTGTCAT CCTCTACATC CAACGCAGAG AAAAGCGAGA ATAGGCGAAC TCTGGACTTA
    CCACGCCTGT CAGAAACCTC CATAAAGGAT CGAATGGCCA AGTACCAGGC AGCTGTGTCC
    AAACAGAGCA GTTCCACCAA CGCTATAAAT GACTTGAGAG CCAATTGTGG TGAAATCAAA
    ACTCATAAAT TGGAGCAAAA GGAGAATGTG CCCCCAGATC CTGAGGTCTG CATCTCCCAT
    CAGGCTGGAG AAAAGGTCTC CGCAGCTGAG AATAGCCTGG CAGTCCATTC CACTCCTGCT
    GAAGATGACT CCCCAGGTAA CTTCCAGGTC AAGAGTGAGG CCCAGCAGCC TGTCCATCCC
    AAGCCACTAA ATCCAGATGC CAGAGCCTCC AGCCTTTCTG AAAGTTCTCC ACCCAAACCA
    GTGAAGAAAT TTCAGGCACC TGCAAGAGAG ACTTGTGTGG AATGTCAGAA GACGGTTTAC
    CCCATGGAAC GTCTCTTCGC TAACCAGCAG GTGTTTCACA TCAGCTGTTT CCGTTGCTCC
    TACTGCAACA ACAAACTTAG TCTAGGAACA TATGCATCTT TACATGGGAG AATCTACTGC
    AAGCCTCACT TCAATCAGCT CTTTAAAGCT AAAGGCAACT ATGATGAAGG CTTTGGGCAC
    AGACCACACA AGGATCTGTG GGCAAGCAAA AATGAAAATG AAGGGACTTT GGAAAGACCA
    GCCCAGCTTC CAAATGTGGG GGAAACCCCT CAGAGCCCAG GGGTAGAAGA TGCCCCCATT
    GCCAAGGTGG GTGTGCTGAC AGCAAGTATG GAAGCCAAGG CCTCCTCTCA GCCAGAGAAA
    GAAGACAAGC CAGCAGAAAC CAAGAAGCTG AAGATCGCCT GGCCGCCCCC CACTGAACTT
    GGTAGTTCAG GAAGTACCCT GGAGGAAGGG ATCAAAGTAT CCAAGCCCAA ATGGCCTCCC
    GAGGATGAAA TGAGCAAGCC TGAAGCTCCC GAGGATGTAG ATCTGGATCT GAAGAAGCTA
    AGACGATCAT CTTCGCTGAA AGAAAGAAGC CGCCCATTCA CTGTAGCAGC TTCATTTCGA
    ACCACTTCAG TCAAGAGCCC AAAAATCTTG TCCCCACCTA TACGGAAAGG CTGGAGCATG
    TCAGAGCAGA GTGAGGAGTT TATGGGAGGA ATGACAGCAG AAAGGAAACA AGTGGAAAAT
    GCCAATACCT CCGAAAAGAA TGGTAGTGTG GGAAAAACAA CCCGGCAAAG CCAGGAGATT
    GGAGGAGGGG AACCAGGGAG GAGAATCAAG GAATATCATG GTTTTGAGAT GGGGAGTGAG
    AATCTTATAG AAAATGGTGC GAACGTAGAT GAAGAGGATA GAAACCTCAA ACAGCAGTCT
    CCACTAGAAC CCAAGTCTAC AAATTGGTCC AGCTTTGCAG ACAATACCTC CCCTAAAGAA
    TTCACGACCC AGAATCAGAA ATCCCAGGAT ATGGGATTCT GGGAGGGAGA ATCAGTCAAA
    GAGCTGTCTG TGGAGGAACA GATAAAGAGA AATCGGTACT ATGATGAGGA CGAGGATGAG
    GATGAGGAAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022266617.1
    CDS42..2333
    Translation

    Target ORF information:

    RefSeq Version XM_022410909.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris LIM domain and actin binding 1 (LIMA1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022410909.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGAATCGA CTCCATTTAA CAGACGGCAA TGGACTTCAG TATCATTGAG GGTAACAGCC 
    AGAGAACTTT CTCTTGTCAA CAAGAATAAG TCATCGGCTA TTGTGGAAAT ATTCTCCAAG
    TACCAGAAAG CAGCTGAAGA AGCAAACATG GAAAAGAGGA GAAGTAACAC TGAAAATCTC
    CCCCAGCACT GCAGGAGGGG GATCCTGACG GTGTTAAAGA AGAAGTGGGA GAACCCAGCA
    CTGGGAACAG AGCCTCACCC AGACTCCCTG CGAAACGGCA GCACTGAGGT CAGACCCGGA
    GGCGACCATC CTCCGGGTGA AGTGGCAAGA AGCTCTGCCT CTGAGGCCGA AGCTGACCAG
    GAAGAAAGAG CCCACCCCAG ACCTAGGCAC GGATCACCTC CTGAAGCCCT AATTCAGTGT
    CCGTATCCCC GCACTGAGGG CAGTGAGCAT CTTAAAGACC CCTCGAAAGA AAGTAAAAAA
    ATGGAAACGT GTGTAGGAGA ACCCAGGCAT GAAGTAGGAA AACCTGAAAT CAGTGAAAAC
    ACAGAAGGCG CAAACAAAAT AGAGAAATAC AATGTTCCGC TGAACAGGCT TAAGATGATG
    TTTGAGAAAG GTGAACCGAC TCAAACCAAG CAGATTCTTC GGACCCAGAG CCGTAGTGCA
    GGTGGGAGGA GGATCTCAGA AAACAGCTAT TCTCTGGATG ATTTGGAAAT AGGCGCAGGT
    CAGGTGTCAT CCTCTACATC CAACGCAGAG AAAAGCGAGA ATAGGCGAAC TCTGGACTTA
    CCACGCCTGT CAGAAACCTC CATAAAGGAT CGAATGGCCA AGTACCAGGC AGCTGTGTCC
    AAACAGAGCA GTTCCACCAA CGCTATAAAT GACTTGAGAG CCAATTGTGG TGAAATCAAA
    ACTCATAAAT TGGAGCAAAA GGAGAATGTG CCCCCAGATC CTGAGGTCTG CATCTCCCAT
    CAGGCTGGAG AAAAGGTCTC CGCAGCTGAG AATAGCCTGG CAGTCCATTC CACTCCTGCT
    GAAGATGACT CCCCAGGTAA CTTCCAGGTC AAGAGTGAGG CCCAGCAGCC TGTCCATCCC
    AAGCCACTAA ATCCAGATGC CAGAGCCTCC AGCCTTTCTG AAAGTTCTCC ACCCAAACCA
    GTGAAGAAAT TTCAGGCACC TGCAAGAGAG ACTTGTGTGG AATGTCAGAA GACGGTTTAC
    CCCATGGAAC GTCTCTTCGC TAACCAGCAG GTGTTTCACA TCAGCTGTTT CCGTTGCTCC
    TACTGCAACA ACAAACTTAG TCTAGGAACA TATGCATCTT TACATGGGAG AATCTACTGC
    AAGCCTCACT TCAATCAGCT CTTTAAAGCT AAAGGCAACT ATGATGAAGG CTTTGGGCAC
    AGACCACACA AGGATCTGTG GGCAAGCAAA AATGAAAATG AAGGGACTTT GGAAAGACCA
    GCCCAGCTTC CAAATGTGGG GGAAACCCCT CAGAGCCCAG GGGTAGAAGA TGCCCCCATT
    GCCAAGGTGG GTGTGCTGAC AGCAAGTATG GAAGCCAAGG CCTCCTCTCA GCCAGAGAAA
    GAAGACAAGC CAGCAGAAAC CAAGAAGCTG AAGATCGCCT GGCCGCCCCC CACTGAACTT
    GGTAGTTCAG GAAGTACCCT GGAGGAAGGG ATCAAAGTAT CCAAGCCCAA ATGGCCTCCC
    GAGGATGAAA TGAGCAAGCC TGAAGCTCCC GAGGATGTAG ATCTGGATCT GAAGAAGCTA
    AGACGATCAT CTTCGCTGAA AGAAAGAAGC CGCCCATTCA CTGTAGCAGC TTCATTTCGA
    ACCACTTCAG TCAAGAGCCC AAAAATCTTG TCCCCACCTA TACGGAAAGG CTGGAGCATG
    TCAGAGCAGA GTGAGGAGTT TATGGGAGGA ATGACAGCAG AAAGGAAACA AGTGGAAAAT
    GCCAATACCT CCGAAAAGAA TGGTAGTGTG GGAAAAACAA CCCGGCAAAG CCAGGAGATT
    GGAGGAGGGG AACCAGGGAG GAGAATCAAG GAATATCATG GTTTTGAGAT GGGGAGTGAG
    AATCTTATAG AAAATGGTGC GAACGTAGAT GAAGAGGATA GAAACCTCAA ACAGCAGTCT
    CCACTAGAAC CCAAGTCTAC AAATTGGTCC AGCTTTGCAG ACAATACCTC CCCTAAAGAA
    TTCACGACCC AGAATCAGAA ATCCCAGGAT ATGGGATTCT GGGAGGGAGA ATCAGTCAAA
    GAGCTGTCTG TGGAGGAACA GATAAAGAGA AATCGGTACT ATGATGAGGA CGAGGATGAG
    GATGAGGAAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe27009
    Clone ID Related Accession (Same CDS sequence) XM_022410909.1 , XM_005636849.3 , XM_005636849.2
    Accession Version XM_005636849.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2292bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product LIM domain and actin-binding protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006609.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005636849.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGAATCGA CTCCATTTAA CAGACGGCAA TGGACTTCAG TATCATTGAG GGTAACAGCC 
    AGAGAACTTT CTCTTGTCAA CAAGAATAAG TCATCGGCTA TTGTGGAAAT ATTCTCCAAG
    TACCAGAAAG CAGCTGAAGA AGCAAACATG GAAAAGAGGA GAAGTAACAC TGAAAATCTC
    CCCCAGCACT GCAGGAGGGG GATCCTGACG GTGTTAAAGA AGAAGTGGGA GAACCCAGCA
    CTGGGAACAG AGCCTCACCC AGACTCCCTG CGAAACGGCA GCACTGAGGT CAGACCCGGA
    GGCGACCATC CTCCGGGTGA AGTGGCAAGA AGCTCTGCCT CTGAGGCCGA AGCTGACCAG
    GAAGAAAGAG CCCACCCCAG ACCTAGGCAC GGATCACCTC CTGAAGCCCT AATTCAGTGT
    CCGTATCCCC GCACTGAGGG CAGTGAGCAT CTTAAAGACC CCTCGAAAGA AAGTAAAAAA
    ATGGAAACGT GTGTAGGAGA ACCCAGGCAT GAAGTAGGAA AACCTGAAAT CAGTGAAAAC
    ACAGAAGGCG CAAACAAAAT AGAGAAATAC AATGTTCCGC TGAACAGGCT TAAGATGATG
    TTTGAGAAAG GTGAACCGAC TCAAACCAAG CAGATTCTTC GGACCCAGAG CCGTAGTGCA
    GGTGGGAGGA GGATCTCAGA AAACAGCTAT TCTCTGGATG ATTTGGAAAT AGGCGCAGGT
    CAGGTGTCAT CCTCTACATC CAACGCAGAG AAAAGCGAGA ATAGGCGAAC TCTGGACTTA
    CCACGCCTGT CAGAAACCTC CATAAAGGAT CGAATGGCCA AGTACCAGGC AGCTGTGTCC
    AAACAGAGCA GTTCCACCAA CGCTATAAAT GACTTGAGAG CCAATTGTGG TGAAATCAAA
    ACTCATAAAT TGGAGCAAAA GGAGAATGTG CCCCCAGATC CTGAGGTCTG CATCTCCCAT
    CAGGCTGGAG AAAAGGTCTC CGCAGCTGAG AATAGCCTGG CAGTCCATTC CACTCCTGCT
    GAAGATGACT CCCCAGGTAA CTTCCAGGTC AAGAGTGAGG CCCAGCAGCC TGTCCATCCC
    AAGCCACTAA ATCCAGATGC CAGAGCCTCC AGCCTTTCTG AAAGTTCTCC ACCCAAACCA
    GTGAAGAAAT TTCAGGCACC TGCAAGAGAG ACTTGTGTGG AATGTCAGAA GACGGTTTAC
    CCCATGGAAC GTCTCTTCGC TAACCAGCAG GTGTTTCACA TCAGCTGTTT CCGTTGCTCC
    TACTGCAACA ACAAACTTAG TCTAGGAACA TATGCATCTT TACATGGGAG AATCTACTGC
    AAGCCTCACT TCAATCAGCT CTTTAAAGCT AAAGGCAACT ATGATGAAGG CTTTGGGCAC
    AGACCACACA AGGATCTGTG GGCAAGCAAA AATGAAAATG AAGGGACTTT GGAAAGACCA
    GCCCAGCTTC CAAATGTGGG GGAAACCCCT CAGAGCCCAG GGGTAGAAGA TGCCCCCATT
    GCCAAGGTGG GTGTGCTGAC AGCAAGTATG GAAGCCAAGG CCTCCTCTCA GCCAGAGAAA
    GAAGACAAGC CAGCAGAAAC CAAGAAGCTG AAGATCGCCT GGCCGCCCCC CACTGAACTT
    GGTAGTTCAG GAAGTACCCT GGAGGAAGGG ATCAAAGTAT CCAAGCCCAA ATGGCCTCCC
    GAGGATGAAA TGAGCAAGCC TGAAGCTCCC GAGGATGTAG ATCTGGATCT GAAGAAGCTA
    AGACGATCAT CTTCGCTGAA AGAAAGAAGC CGCCCATTCA CTGTAGCAGC TTCATTTCGA
    ACCACTTCAG TCAAGAGCCC AAAAATCTTG TCCCCACCTA TACGGAAAGG CTGGAGCATG
    TCAGAGCAGA GTGAGGAGTT TATGGGAGGA ATGACAGCAG AAAGGAAACA AGTGGAAAAT
    GCCAATACCT CCGAAAAGAA TGGTAGTGTG GGAAAAACAA CCCGGCAAAG CCAGGAGATT
    GGAGGAGGGG AACCAGGGAG GAGAATCAAG GAATATCATG GTTTTGAGAT GGGGAGTGAG
    AATCTTATAG AAAATGGTGC GAACGTAGAT GAAGAGGATA GAAACCTCAA ACAGCAGTCT
    CCACTAGAAC CCAAGTCTAC AAATTGGTCC AGCTTTGCAG ACAATACCTC CCCTAAAGAA
    TTCACGACCC AGAATCAGAA ATCCCAGGAT ATGGGATTCT GGGAGGGAGA ATCAGTCAAA
    GAGCTGTCTG TGGAGGAACA GATAAAGAGA AATCGGTACT ATGATGAGGA CGAGGATGAG
    GATGAGGAAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005636906.1
    CDS153..2444
    Translation

    Target ORF information:

    RefSeq Version XM_005636849.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris LIM domain and actin binding 1 (LIMA1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005636849.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGAATCGA CTCCATTTAA CAGACGGCAA TGGACTTCAG TATCATTGAG GGTAACAGCC 
    AGAGAACTTT CTCTTGTCAA CAAGAATAAG TCATCGGCTA TTGTGGAAAT ATTCTCCAAG
    TACCAGAAAG CAGCTGAAGA AGCAAACATG GAAAAGAGGA GAAGTAACAC TGAAAATCTC
    CCCCAGCACT GCAGGAGGGG GATCCTGACG GTGTTAAAGA AGAAGTGGGA GAACCCAGCA
    CTGGGAACAG AGCCTCACCC AGACTCCCTG CGAAACGGCA GCACTGAGGT CAGACCCGGA
    GGCGACCATC CTCCGGGTGA AGTGGCAAGA AGCTCTGCCT CTGAGGCCGA AGCTGACCAG
    GAAGAAAGAG CCCACCCCAG ACCTAGGCAC GGATCACCTC CTGAAGCCCT AATTCAGTGT
    CCGTATCCCC GCACTGAGGG CAGTGAGCAT CTTAAAGACC CCTCGAAAGA AAGTAAAAAA
    ATGGAAACGT GTGTAGGAGA ACCCAGGCAT GAAGTAGGAA AACCTGAAAT CAGTGAAAAC
    ACAGAAGGCG CAAACAAAAT AGAGAAATAC AATGTTCCGC TGAACAGGCT TAAGATGATG
    TTTGAGAAAG GTGAACCGAC TCAAACCAAG CAGATTCTTC GGACCCAGAG CCGTAGTGCA
    GGTGGGAGGA GGATCTCAGA AAACAGCTAT TCTCTGGATG ATTTGGAAAT AGGCGCAGGT
    CAGGTGTCAT CCTCTACATC CAACGCAGAG AAAAGCGAGA ATAGGCGAAC TCTGGACTTA
    CCACGCCTGT CAGAAACCTC CATAAAGGAT CGAATGGCCA AGTACCAGGC AGCTGTGTCC
    AAACAGAGCA GTTCCACCAA CGCTATAAAT GACTTGAGAG CCAATTGTGG TGAAATCAAA
    ACTCATAAAT TGGAGCAAAA GGAGAATGTG CCCCCAGATC CTGAGGTCTG CATCTCCCAT
    CAGGCTGGAG AAAAGGTCTC CGCAGCTGAG AATAGCCTGG CAGTCCATTC CACTCCTGCT
    GAAGATGACT CCCCAGGTAA CTTCCAGGTC AAGAGTGAGG CCCAGCAGCC TGTCCATCCC
    AAGCCACTAA ATCCAGATGC CAGAGCCTCC AGCCTTTCTG AAAGTTCTCC ACCCAAACCA
    GTGAAGAAAT TTCAGGCACC TGCAAGAGAG ACTTGTGTGG AATGTCAGAA GACGGTTTAC
    CCCATGGAAC GTCTCTTCGC TAACCAGCAG GTGTTTCACA TCAGCTGTTT CCGTTGCTCC
    TACTGCAACA ACAAACTTAG TCTAGGAACA TATGCATCTT TACATGGGAG AATCTACTGC
    AAGCCTCACT TCAATCAGCT CTTTAAAGCT AAAGGCAACT ATGATGAAGG CTTTGGGCAC
    AGACCACACA AGGATCTGTG GGCAAGCAAA AATGAAAATG AAGGGACTTT GGAAAGACCA
    GCCCAGCTTC CAAATGTGGG GGAAACCCCT CAGAGCCCAG GGGTAGAAGA TGCCCCCATT
    GCCAAGGTGG GTGTGCTGAC AGCAAGTATG GAAGCCAAGG CCTCCTCTCA GCCAGAGAAA
    GAAGACAAGC CAGCAGAAAC CAAGAAGCTG AAGATCGCCT GGCCGCCCCC CACTGAACTT
    GGTAGTTCAG GAAGTACCCT GGAGGAAGGG ATCAAAGTAT CCAAGCCCAA ATGGCCTCCC
    GAGGATGAAA TGAGCAAGCC TGAAGCTCCC GAGGATGTAG ATCTGGATCT GAAGAAGCTA
    AGACGATCAT CTTCGCTGAA AGAAAGAAGC CGCCCATTCA CTGTAGCAGC TTCATTTCGA
    ACCACTTCAG TCAAGAGCCC AAAAATCTTG TCCCCACCTA TACGGAAAGG CTGGAGCATG
    TCAGAGCAGA GTGAGGAGTT TATGGGAGGA ATGACAGCAG AAAGGAAACA AGTGGAAAAT
    GCCAATACCT CCGAAAAGAA TGGTAGTGTG GGAAAAACAA CCCGGCAAAG CCAGGAGATT
    GGAGGAGGGG AACCAGGGAG GAGAATCAAG GAATATCATG GTTTTGAGAT GGGGAGTGAG
    AATCTTATAG AAAATGGTGC GAACGTAGAT GAAGAGGATA GAAACCTCAA ACAGCAGTCT
    CCACTAGAAC CCAAGTCTAC AAATTGGTCC AGCTTTGCAG ACAATACCTC CCCTAAAGAA
    TTCACGACCC AGAATCAGAA ATCCCAGGAT ATGGGATTCT GGGAGGGAGA ATCAGTCAAA
    GAGCTGTCTG TGGAGGAACA GATAAAGAGA AATCGGTACT ATGATGAGGA CGAGGATGAG
    GATGAGGAAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe27009
    Clone ID Related Accession (Same CDS sequence) XM_022410909.1 , XM_005636849.3 , XM_005636849.2
    Accession Version XM_005636849.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2292bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product LIM domain and actin-binding protein 1 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726108.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005636849.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGAATCGA CTCCATTTAA CAGACGGCAA TGGACTTCAG TATCATTGAG GGTAACAGCC 
    AGAGAACTTT CTCTTGTCAA CAAGAATAAG TCATCGGCTA TTGTGGAAAT ATTCTCCAAG
    TACCAGAAAG CAGCTGAAGA AGCAAACATG GAAAAGAGGA GAAGTAACAC TGAAAATCTC
    CCCCAGCACT GCAGGAGGGG GATCCTGACG GTGTTAAAGA AGAAGTGGGA GAACCCAGCA
    CTGGGAACAG AGCCTCACCC AGACTCCCTG CGAAACGGCA GCACTGAGGT CAGACCCGGA
    GGCGACCATC CTCCGGGTGA AGTGGCAAGA AGCTCTGCCT CTGAGGCCGA AGCTGACCAG
    GAAGAAAGAG CCCACCCCAG ACCTAGGCAC GGATCACCTC CTGAAGCCCT AATTCAGTGT
    CCGTATCCCC GCACTGAGGG CAGTGAGCAT CTTAAAGACC CCTCGAAAGA AAGTAAAAAA
    ATGGAAACGT GTGTAGGAGA ACCCAGGCAT GAAGTAGGAA AACCTGAAAT CAGTGAAAAC
    ACAGAAGGCG CAAACAAAAT AGAGAAATAC AATGTTCCGC TGAACAGGCT TAAGATGATG
    TTTGAGAAAG GTGAACCGAC TCAAACCAAG CAGATTCTTC GGACCCAGAG CCGTAGTGCA
    GGTGGGAGGA GGATCTCAGA AAACAGCTAT TCTCTGGATG ATTTGGAAAT AGGCGCAGGT
    CAGGTGTCAT CCTCTACATC CAACGCAGAG AAAAGCGAGA ATAGGCGAAC TCTGGACTTA
    CCACGCCTGT CAGAAACCTC CATAAAGGAT CGAATGGCCA AGTACCAGGC AGCTGTGTCC
    AAACAGAGCA GTTCCACCAA CGCTATAAAT GACTTGAGAG CCAATTGTGG TGAAATCAAA
    ACTCATAAAT TGGAGCAAAA GGAGAATGTG CCCCCAGATC CTGAGGTCTG CATCTCCCAT
    CAGGCTGGAG AAAAGGTCTC CGCAGCTGAG AATAGCCTGG CAGTCCATTC CACTCCTGCT
    GAAGATGACT CCCCAGGTAA CTTCCAGGTC AAGAGTGAGG CCCAGCAGCC TGTCCATCCC
    AAGCCACTAA ATCCAGATGC CAGAGCCTCC AGCCTTTCTG AAAGTTCTCC ACCCAAACCA
    GTGAAGAAAT TTCAGGCACC TGCAAGAGAG ACTTGTGTGG AATGTCAGAA GACGGTTTAC
    CCCATGGAAC GTCTCTTCGC TAACCAGCAG GTGTTTCACA TCAGCTGTTT CCGTTGCTCC
    TACTGCAACA ACAAACTTAG TCTAGGAACA TATGCATCTT TACATGGGAG AATCTACTGC
    AAGCCTCACT TCAATCAGCT CTTTAAAGCT AAAGGCAACT ATGATGAAGG CTTTGGGCAC
    AGACCACACA AGGATCTGTG GGCAAGCAAA AATGAAAATG AAGGGACTTT GGAAAGACCA
    GCCCAGCTTC CAAATGTGGG GGAAACCCCT CAGAGCCCAG GGGTAGAAGA TGCCCCCATT
    GCCAAGGTGG GTGTGCTGAC AGCAAGTATG GAAGCCAAGG CCTCCTCTCA GCCAGAGAAA
    GAAGACAAGC CAGCAGAAAC CAAGAAGCTG AAGATCGCCT GGCCGCCCCC CACTGAACTT
    GGTAGTTCAG GAAGTACCCT GGAGGAAGGG ATCAAAGTAT CCAAGCCCAA ATGGCCTCCC
    GAGGATGAAA TGAGCAAGCC TGAAGCTCCC GAGGATGTAG ATCTGGATCT GAAGAAGCTA
    AGACGATCAT CTTCGCTGAA AGAAAGAAGC CGCCCATTCA CTGTAGCAGC TTCATTTCGA
    ACCACTTCAG TCAAGAGCCC AAAAATCTTG TCCCCACCTA TACGGAAAGG CTGGAGCATG
    TCAGAGCAGA GTGAGGAGTT TATGGGAGGA ATGACAGCAG AAAGGAAACA AGTGGAAAAT
    GCCAATACCT CCGAAAAGAA TGGTAGTGTG GGAAAAACAA CCCGGCAAAG CCAGGAGATT
    GGAGGAGGGG AACCAGGGAG GAGAATCAAG GAATATCATG GTTTTGAGAT GGGGAGTGAG
    AATCTTATAG AAAATGGTGC GAACGTAGAT GAAGAGGATA GAAACCTCAA ACAGCAGTCT
    CCACTAGAAC CCAAGTCTAC AAATTGGTCC AGCTTTGCAG ACAATACCTC CCCTAAAGAA
    TTCACGACCC AGAATCAGAA ATCCCAGGAT ATGGGATTCT GGGAGGGAGA ATCAGTCAAA
    GAGCTGTCTG TGGAGGAACA GATAAAGAGA AATCGGTACT ATGATGAGGA CGAGGATGAG
    GATGAGGAAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005636906.1
    CDS138..2429
    Translation

    Target ORF information:

    RefSeq Version XM_005636849.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris LIM domain and actin binding 1 (LIMA1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005636849.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGAATCGA CTCCATTTAA CAGACGGCAA TGGACTTCAG TATCATTGAG GGTAACAGCC 
    AGAGAACTTT CTCTTGTCAA CAAGAATAAG TCATCGGCTA TTGTGGAAAT ATTCTCCAAG
    TACCAGAAAG CAGCTGAAGA AGCAAACATG GAAAAGAGGA GAAGTAACAC TGAAAATCTC
    CCCCAGCACT GCAGGAGGGG GATCCTGACG GTGTTAAAGA AGAAGTGGGA GAACCCAGCA
    CTGGGAACAG AGCCTCACCC AGACTCCCTG CGAAACGGCA GCACTGAGGT CAGACCCGGA
    GGCGACCATC CTCCGGGTGA AGTGGCAAGA AGCTCTGCCT CTGAGGCCGA AGCTGACCAG
    GAAGAAAGAG CCCACCCCAG ACCTAGGCAC GGATCACCTC CTGAAGCCCT AATTCAGTGT
    CCGTATCCCC GCACTGAGGG CAGTGAGCAT CTTAAAGACC CCTCGAAAGA AAGTAAAAAA
    ATGGAAACGT GTGTAGGAGA ACCCAGGCAT GAAGTAGGAA AACCTGAAAT CAGTGAAAAC
    ACAGAAGGCG CAAACAAAAT AGAGAAATAC AATGTTCCGC TGAACAGGCT TAAGATGATG
    TTTGAGAAAG GTGAACCGAC TCAAACCAAG CAGATTCTTC GGACCCAGAG CCGTAGTGCA
    GGTGGGAGGA GGATCTCAGA AAACAGCTAT TCTCTGGATG ATTTGGAAAT AGGCGCAGGT
    CAGGTGTCAT CCTCTACATC CAACGCAGAG AAAAGCGAGA ATAGGCGAAC TCTGGACTTA
    CCACGCCTGT CAGAAACCTC CATAAAGGAT CGAATGGCCA AGTACCAGGC AGCTGTGTCC
    AAACAGAGCA GTTCCACCAA CGCTATAAAT GACTTGAGAG CCAATTGTGG TGAAATCAAA
    ACTCATAAAT TGGAGCAAAA GGAGAATGTG CCCCCAGATC CTGAGGTCTG CATCTCCCAT
    CAGGCTGGAG AAAAGGTCTC CGCAGCTGAG AATAGCCTGG CAGTCCATTC CACTCCTGCT
    GAAGATGACT CCCCAGGTAA CTTCCAGGTC AAGAGTGAGG CCCAGCAGCC TGTCCATCCC
    AAGCCACTAA ATCCAGATGC CAGAGCCTCC AGCCTTTCTG AAAGTTCTCC ACCCAAACCA
    GTGAAGAAAT TTCAGGCACC TGCAAGAGAG ACTTGTGTGG AATGTCAGAA GACGGTTTAC
    CCCATGGAAC GTCTCTTCGC TAACCAGCAG GTGTTTCACA TCAGCTGTTT CCGTTGCTCC
    TACTGCAACA ACAAACTTAG TCTAGGAACA TATGCATCTT TACATGGGAG AATCTACTGC
    AAGCCTCACT TCAATCAGCT CTTTAAAGCT AAAGGCAACT ATGATGAAGG CTTTGGGCAC
    AGACCACACA AGGATCTGTG GGCAAGCAAA AATGAAAATG AAGGGACTTT GGAAAGACCA
    GCCCAGCTTC CAAATGTGGG GGAAACCCCT CAGAGCCCAG GGGTAGAAGA TGCCCCCATT
    GCCAAGGTGG GTGTGCTGAC AGCAAGTATG GAAGCCAAGG CCTCCTCTCA GCCAGAGAAA
    GAAGACAAGC CAGCAGAAAC CAAGAAGCTG AAGATCGCCT GGCCGCCCCC CACTGAACTT
    GGTAGTTCAG GAAGTACCCT GGAGGAAGGG ATCAAAGTAT CCAAGCCCAA ATGGCCTCCC
    GAGGATGAAA TGAGCAAGCC TGAAGCTCCC GAGGATGTAG ATCTGGATCT GAAGAAGCTA
    AGACGATCAT CTTCGCTGAA AGAAAGAAGC CGCCCATTCA CTGTAGCAGC TTCATTTCGA
    ACCACTTCAG TCAAGAGCCC AAAAATCTTG TCCCCACCTA TACGGAAAGG CTGGAGCATG
    TCAGAGCAGA GTGAGGAGTT TATGGGAGGA ATGACAGCAG AAAGGAAACA AGTGGAAAAT
    GCCAATACCT CCGAAAAGAA TGGTAGTGTG GGAAAAACAA CCCGGCAAAG CCAGGAGATT
    GGAGGAGGGG AACCAGGGAG GAGAATCAAG GAATATCATG GTTTTGAGAT GGGGAGTGAG
    AATCTTATAG AAAATGGTGC GAACGTAGAT GAAGAGGATA GAAACCTCAA ACAGCAGTCT
    CCACTAGAAC CCAAGTCTAC AAATTGGTCC AGCTTTGCAG ACAATACCTC CCCTAAAGAA
    TTCACGACCC AGAATCAGAA ATCCCAGGAT ATGGGATTCT GGGAGGGAGA ATCAGTCAAA
    GAGCTGTCTG TGGAGGAACA GATAAAGAGA AATCGGTACT ATGATGAGGA CGAGGATGAG
    GATGAGGAAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe27010
    Clone ID Related Accession (Same CDS sequence) XM_005636850.3 , XM_005636850.2
    Accession Version XM_005636850.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2289bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product LIM domain and actin-binding protein 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006609.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005636850.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGAATCGA CTCCATTTAA CAGACGGCAA TGGACTTCAG TATCATTGAG GGTAACAGCC 
    AGAGAACTTT CTCTTGTCAA CAAGAATAAG TCATCGGCTA TTGTGGAAAT ATTCTCCAAG
    TACCAGAAAG CAGCTGAAGA AGCAAACATG GAAAAGAGGA GAAGTAACAC TGAAAATCTC
    CCCCAGCACT GCAGGAGGGG GATCCTGACG GTGTTAAAGA AGAAGTGGGA GAACCCAGCA
    CTGGGAACAG AGCCTCACCC AGACTCCCTG CGAAACGGCA GCACTGAGGT CAGACCCGGA
    GGCGACCATC CTCCGGGTGA AGTGGCAAGA AGCTCTGCCT CTGAGGCCGA AGCTGACCAG
    GAAGAAAGAG CCCACCCCAG ACCTAGGCAC GGATCACCTC CTGAAGCCCT AATTCAGTGT
    CCGTATCCCC GCACTGAGGG CAGTGAGCAT CTTAAAGACC CCTCGAAAGA AAGTAAAAAA
    ATGGAAACGT GTGTAGGAGA ACCCAGGCAT GAAGTAGGAA AACCTGAAAT CAGTGAAAAC
    ACAGAAGGCG CAAACAAAAT AGAGAAATAC AATGTTCCGC TGAACAGGCT TAAGATGATG
    TTTGAGAAAG GTGAACCGAC TCAAACCAAG CAGATTCTTC GGACCCAGAG CCGTAGTGCA
    GGTGGGAGGA GGATCTCAGA AAACAGCTAT TCTCTGGATG ATTTGGAAAT AGGCGCAGGT
    CAGGTGTCAT CCTCTACATC CAACGCAGAG AAAAGCGAGA ATAGGCGAAC TCTGGACTTA
    CCACGCCTGT CAGAAACCTC CATAAAGGAT CGAATGGCCA AGTACCAGGC AGCTGTGTCC
    AAACAGAGCA GTTCCACCAA CGCTATAAAT GACTTGAGAG CCAATTGTGG TGAAATCAAA
    ACTCATAAAT TGGAGCAAAA GGAGAATGTG CCCCCAGATC CTGAGGTCTG CATCTCCCAT
    CAGGCTGGAG AAAAGGTCTC CGCAGCTGAG AATAGCCTGG CAGTCCATTC CACTCCTGCT
    GAAGATGACT CCCGTAACTT CCAGGTCAAG AGTGAGGCCC AGCAGCCTGT CCATCCCAAG
    CCACTAAATC CAGATGCCAG AGCCTCCAGC CTTTCTGAAA GTTCTCCACC CAAACCAGTG
    AAGAAATTTC AGGCACCTGC AAGAGAGACT TGTGTGGAAT GTCAGAAGAC GGTTTACCCC
    ATGGAACGTC TCTTCGCTAA CCAGCAGGTG TTTCACATCA GCTGTTTCCG TTGCTCCTAC
    TGCAACAACA AACTTAGTCT AGGAACATAT GCATCTTTAC ATGGGAGAAT CTACTGCAAG
    CCTCACTTCA ATCAGCTCTT TAAAGCTAAA GGCAACTATG ATGAAGGCTT TGGGCACAGA
    CCACACAAGG ATCTGTGGGC AAGCAAAAAT GAAAATGAAG GGACTTTGGA AAGACCAGCC
    CAGCTTCCAA ATGTGGGGGA AACCCCTCAG AGCCCAGGGG TAGAAGATGC CCCCATTGCC
    AAGGTGGGTG TGCTGACAGC AAGTATGGAA GCCAAGGCCT CCTCTCAGCC AGAGAAAGAA
    GACAAGCCAG CAGAAACCAA GAAGCTGAAG ATCGCCTGGC CGCCCCCCAC TGAACTTGGT
    AGTTCAGGAA GTACCCTGGA GGAAGGGATC AAAGTATCCA AGCCCAAATG GCCTCCCGAG
    GATGAAATGA GCAAGCCTGA AGCTCCCGAG GATGTAGATC TGGATCTGAA GAAGCTAAGA
    CGATCATCTT CGCTGAAAGA AAGAAGCCGC CCATTCACTG TAGCAGCTTC ATTTCGAACC
    ACTTCAGTCA AGAGCCCAAA AATCTTGTCC CCACCTATAC GGAAAGGCTG GAGCATGTCA
    GAGCAGAGTG AGGAGTTTAT GGGAGGAATG ACAGCAGAAA GGAAACAAGT GGAAAATGCC
    AATACCTCCG AAAAGAATGG TAGTGTGGGA AAAACAACCC GGCAAAGCCA GGAGATTGGA
    GGAGGGGAAC CAGGGAGGAG AATCAAGGAA TATCATGGTT TTGAGATGGG GAGTGAGAAT
    CTTATAGAAA ATGGTGCGAA CGTAGATGAA GAGGATAGAA ACCTCAAACA GCAGTCTCCA
    CTAGAACCCA AGTCTACAAA TTGGTCCAGC TTTGCAGACA ATACCTCCCC TAAAGAATTC
    ACGACCCAGA ATCAGAAATC CCAGGATATG GGATTCTGGG AGGGAGAATC AGTCAAAGAG
    CTGTCTGTGG AGGAACAGAT AAAGAGAAAT CGGTACTATG ATGAGGACGA GGATGAGGAT
    GAGGAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005636907.1
    CDS153..2441
    Translation

    Target ORF information:

    RefSeq Version XM_005636850.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris LIM domain and actin binding 1 (LIMA1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005636850.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGAATCGA CTCCATTTAA CAGACGGCAA TGGACTTCAG TATCATTGAG GGTAACAGCC 
    AGAGAACTTT CTCTTGTCAA CAAGAATAAG TCATCGGCTA TTGTGGAAAT ATTCTCCAAG
    TACCAGAAAG CAGCTGAAGA AGCAAACATG GAAAAGAGGA GAAGTAACAC TGAAAATCTC
    CCCCAGCACT GCAGGAGGGG GATCCTGACG GTGTTAAAGA AGAAGTGGGA GAACCCAGCA
    CTGGGAACAG AGCCTCACCC AGACTCCCTG CGAAACGGCA GCACTGAGGT CAGACCCGGA
    GGCGACCATC CTCCGGGTGA AGTGGCAAGA AGCTCTGCCT CTGAGGCCGA AGCTGACCAG
    GAAGAAAGAG CCCACCCCAG ACCTAGGCAC GGATCACCTC CTGAAGCCCT AATTCAGTGT
    CCGTATCCCC GCACTGAGGG CAGTGAGCAT CTTAAAGACC CCTCGAAAGA AAGTAAAAAA
    ATGGAAACGT GTGTAGGAGA ACCCAGGCAT GAAGTAGGAA AACCTGAAAT CAGTGAAAAC
    ACAGAAGGCG CAAACAAAAT AGAGAAATAC AATGTTCCGC TGAACAGGCT TAAGATGATG
    TTTGAGAAAG GTGAACCGAC TCAAACCAAG CAGATTCTTC GGACCCAGAG CCGTAGTGCA
    GGTGGGAGGA GGATCTCAGA AAACAGCTAT TCTCTGGATG ATTTGGAAAT AGGCGCAGGT
    CAGGTGTCAT CCTCTACATC CAACGCAGAG AAAAGCGAGA ATAGGCGAAC TCTGGACTTA
    CCACGCCTGT CAGAAACCTC CATAAAGGAT CGAATGGCCA AGTACCAGGC AGCTGTGTCC
    AAACAGAGCA GTTCCACCAA CGCTATAAAT GACTTGAGAG CCAATTGTGG TGAAATCAAA
    ACTCATAAAT TGGAGCAAAA GGAGAATGTG CCCCCAGATC CTGAGGTCTG CATCTCCCAT
    CAGGCTGGAG AAAAGGTCTC CGCAGCTGAG AATAGCCTGG CAGTCCATTC CACTCCTGCT
    GAAGATGACT CCCGTAACTT CCAGGTCAAG AGTGAGGCCC AGCAGCCTGT CCATCCCAAG
    CCACTAAATC CAGATGCCAG AGCCTCCAGC CTTTCTGAAA GTTCTCCACC CAAACCAGTG
    AAGAAATTTC AGGCACCTGC AAGAGAGACT TGTGTGGAAT GTCAGAAGAC GGTTTACCCC
    ATGGAACGTC TCTTCGCTAA CCAGCAGGTG TTTCACATCA GCTGTTTCCG TTGCTCCTAC
    TGCAACAACA AACTTAGTCT AGGAACATAT GCATCTTTAC ATGGGAGAAT CTACTGCAAG
    CCTCACTTCA ATCAGCTCTT TAAAGCTAAA GGCAACTATG ATGAAGGCTT TGGGCACAGA
    CCACACAAGG ATCTGTGGGC AAGCAAAAAT GAAAATGAAG GGACTTTGGA AAGACCAGCC
    CAGCTTCCAA ATGTGGGGGA AACCCCTCAG AGCCCAGGGG TAGAAGATGC CCCCATTGCC
    AAGGTGGGTG TGCTGACAGC AAGTATGGAA GCCAAGGCCT CCTCTCAGCC AGAGAAAGAA
    GACAAGCCAG CAGAAACCAA GAAGCTGAAG ATCGCCTGGC CGCCCCCCAC TGAACTTGGT
    AGTTCAGGAA GTACCCTGGA GGAAGGGATC AAAGTATCCA AGCCCAAATG GCCTCCCGAG
    GATGAAATGA GCAAGCCTGA AGCTCCCGAG GATGTAGATC TGGATCTGAA GAAGCTAAGA
    CGATCATCTT CGCTGAAAGA AAGAAGCCGC CCATTCACTG TAGCAGCTTC ATTTCGAACC
    ACTTCAGTCA AGAGCCCAAA AATCTTGTCC CCACCTATAC GGAAAGGCTG GAGCATGTCA
    GAGCAGAGTG AGGAGTTTAT GGGAGGAATG ACAGCAGAAA GGAAACAAGT GGAAAATGCC
    AATACCTCCG AAAAGAATGG TAGTGTGGGA AAAACAACCC GGCAAAGCCA GGAGATTGGA
    GGAGGGGAAC CAGGGAGGAG AATCAAGGAA TATCATGGTT TTGAGATGGG GAGTGAGAAT
    CTTATAGAAA ATGGTGCGAA CGTAGATGAA GAGGATAGAA ACCTCAAACA GCAGTCTCCA
    CTAGAACCCA AGTCTACAAA TTGGTCCAGC TTTGCAGACA ATACCTCCCC TAAAGAATTC
    ACGACCCAGA ATCAGAAATC CCAGGATATG GGATTCTGGG AGGGAGAATC AGTCAAAGAG
    CTGTCTGTGG AGGAACAGAT AAAGAGAAAT CGGTACTATG ATGAGGACGA GGATGAGGAT
    GAGGAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe27010
    Clone ID Related Accession (Same CDS sequence) XM_005636850.3 , XM_005636850.2
    Accession Version XM_005636850.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2289bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product LIM domain and actin-binding protein 1 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726108.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005636850.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGAATCGA CTCCATTTAA CAGACGGCAA TGGACTTCAG TATCATTGAG GGTAACAGCC 
    AGAGAACTTT CTCTTGTCAA CAAGAATAAG TCATCGGCTA TTGTGGAAAT ATTCTCCAAG
    TACCAGAAAG CAGCTGAAGA AGCAAACATG GAAAAGAGGA GAAGTAACAC TGAAAATCTC
    CCCCAGCACT GCAGGAGGGG GATCCTGACG GTGTTAAAGA AGAAGTGGGA GAACCCAGCA
    CTGGGAACAG AGCCTCACCC AGACTCCCTG CGAAACGGCA GCACTGAGGT CAGACCCGGA
    GGCGACCATC CTCCGGGTGA AGTGGCAAGA AGCTCTGCCT CTGAGGCCGA AGCTGACCAG
    GAAGAAAGAG CCCACCCCAG ACCTAGGCAC GGATCACCTC CTGAAGCCCT AATTCAGTGT
    CCGTATCCCC GCACTGAGGG CAGTGAGCAT CTTAAAGACC CCTCGAAAGA AAGTAAAAAA
    ATGGAAACGT GTGTAGGAGA ACCCAGGCAT GAAGTAGGAA AACCTGAAAT CAGTGAAAAC
    ACAGAAGGCG CAAACAAAAT AGAGAAATAC AATGTTCCGC TGAACAGGCT TAAGATGATG
    TTTGAGAAAG GTGAACCGAC TCAAACCAAG CAGATTCTTC GGACCCAGAG CCGTAGTGCA
    GGTGGGAGGA GGATCTCAGA AAACAGCTAT TCTCTGGATG ATTTGGAAAT AGGCGCAGGT
    CAGGTGTCAT CCTCTACATC CAACGCAGAG AAAAGCGAGA ATAGGCGAAC TCTGGACTTA
    CCACGCCTGT CAGAAACCTC CATAAAGGAT CGAATGGCCA AGTACCAGGC AGCTGTGTCC
    AAACAGAGCA GTTCCACCAA CGCTATAAAT GACTTGAGAG CCAATTGTGG TGAAATCAAA
    ACTCATAAAT TGGAGCAAAA GGAGAATGTG CCCCCAGATC CTGAGGTCTG CATCTCCCAT
    CAGGCTGGAG AAAAGGTCTC CGCAGCTGAG AATAGCCTGG CAGTCCATTC CACTCCTGCT
    GAAGATGACT CCCGTAACTT CCAGGTCAAG AGTGAGGCCC AGCAGCCTGT CCATCCCAAG
    CCACTAAATC CAGATGCCAG AGCCTCCAGC CTTTCTGAAA GTTCTCCACC CAAACCAGTG
    AAGAAATTTC AGGCACCTGC AAGAGAGACT TGTGTGGAAT GTCAGAAGAC GGTTTACCCC
    ATGGAACGTC TCTTCGCTAA CCAGCAGGTG TTTCACATCA GCTGTTTCCG TTGCTCCTAC
    TGCAACAACA AACTTAGTCT AGGAACATAT GCATCTTTAC ATGGGAGAAT CTACTGCAAG
    CCTCACTTCA ATCAGCTCTT TAAAGCTAAA GGCAACTATG ATGAAGGCTT TGGGCACAGA
    CCACACAAGG ATCTGTGGGC AAGCAAAAAT GAAAATGAAG GGACTTTGGA AAGACCAGCC
    CAGCTTCCAA ATGTGGGGGA AACCCCTCAG AGCCCAGGGG TAGAAGATGC CCCCATTGCC
    AAGGTGGGTG TGCTGACAGC AAGTATGGAA GCCAAGGCCT CCTCTCAGCC AGAGAAAGAA
    GACAAGCCAG CAGAAACCAA GAAGCTGAAG ATCGCCTGGC CGCCCCCCAC TGAACTTGGT
    AGTTCAGGAA GTACCCTGGA GGAAGGGATC AAAGTATCCA AGCCCAAATG GCCTCCCGAG
    GATGAAATGA GCAAGCCTGA AGCTCCCGAG GATGTAGATC TGGATCTGAA GAAGCTAAGA
    CGATCATCTT CGCTGAAAGA AAGAAGCCGC CCATTCACTG TAGCAGCTTC ATTTCGAACC
    ACTTCAGTCA AGAGCCCAAA AATCTTGTCC CCACCTATAC GGAAAGGCTG GAGCATGTCA
    GAGCAGAGTG AGGAGTTTAT GGGAGGAATG ACAGCAGAAA GGAAACAAGT GGAAAATGCC
    AATACCTCCG AAAAGAATGG TAGTGTGGGA AAAACAACCC GGCAAAGCCA GGAGATTGGA
    GGAGGGGAAC CAGGGAGGAG AATCAAGGAA TATCATGGTT TTGAGATGGG GAGTGAGAAT
    CTTATAGAAA ATGGTGCGAA CGTAGATGAA GAGGATAGAA ACCTCAAACA GCAGTCTCCA
    CTAGAACCCA AGTCTACAAA TTGGTCCAGC TTTGCAGACA ATACCTCCCC TAAAGAATTC
    ACGACCCAGA ATCAGAAATC CCAGGATATG GGATTCTGGG AGGGAGAATC AGTCAAAGAG
    CTGTCTGTGG AGGAACAGAT AAAGAGAAAT CGGTACTATG ATGAGGACGA GGATGAGGAT
    GAGGAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005636907.1
    CDS138..2426
    Translation

    Target ORF information:

    RefSeq Version XM_005636850.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris LIM domain and actin binding 1 (LIMA1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005636850.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGAATCGA CTCCATTTAA CAGACGGCAA TGGACTTCAG TATCATTGAG GGTAACAGCC 
    AGAGAACTTT CTCTTGTCAA CAAGAATAAG TCATCGGCTA TTGTGGAAAT ATTCTCCAAG
    TACCAGAAAG CAGCTGAAGA AGCAAACATG GAAAAGAGGA GAAGTAACAC TGAAAATCTC
    CCCCAGCACT GCAGGAGGGG GATCCTGACG GTGTTAAAGA AGAAGTGGGA GAACCCAGCA
    CTGGGAACAG AGCCTCACCC AGACTCCCTG CGAAACGGCA GCACTGAGGT CAGACCCGGA
    GGCGACCATC CTCCGGGTGA AGTGGCAAGA AGCTCTGCCT CTGAGGCCGA AGCTGACCAG
    GAAGAAAGAG CCCACCCCAG ACCTAGGCAC GGATCACCTC CTGAAGCCCT AATTCAGTGT
    CCGTATCCCC GCACTGAGGG CAGTGAGCAT CTTAAAGACC CCTCGAAAGA AAGTAAAAAA
    ATGGAAACGT GTGTAGGAGA ACCCAGGCAT GAAGTAGGAA AACCTGAAAT CAGTGAAAAC
    ACAGAAGGCG CAAACAAAAT AGAGAAATAC AATGTTCCGC TGAACAGGCT TAAGATGATG
    TTTGAGAAAG GTGAACCGAC TCAAACCAAG CAGATTCTTC GGACCCAGAG CCGTAGTGCA
    GGTGGGAGGA GGATCTCAGA AAACAGCTAT TCTCTGGATG ATTTGGAAAT AGGCGCAGGT
    CAGGTGTCAT CCTCTACATC CAACGCAGAG AAAAGCGAGA ATAGGCGAAC TCTGGACTTA
    CCACGCCTGT CAGAAACCTC CATAAAGGAT CGAATGGCCA AGTACCAGGC AGCTGTGTCC
    AAACAGAGCA GTTCCACCAA CGCTATAAAT GACTTGAGAG CCAATTGTGG TGAAATCAAA
    ACTCATAAAT TGGAGCAAAA GGAGAATGTG CCCCCAGATC CTGAGGTCTG CATCTCCCAT
    CAGGCTGGAG AAAAGGTCTC CGCAGCTGAG AATAGCCTGG CAGTCCATTC CACTCCTGCT
    GAAGATGACT CCCGTAACTT CCAGGTCAAG AGTGAGGCCC AGCAGCCTGT CCATCCCAAG
    CCACTAAATC CAGATGCCAG AGCCTCCAGC CTTTCTGAAA GTTCTCCACC CAAACCAGTG
    AAGAAATTTC AGGCACCTGC AAGAGAGACT TGTGTGGAAT GTCAGAAGAC GGTTTACCCC
    ATGGAACGTC TCTTCGCTAA CCAGCAGGTG TTTCACATCA GCTGTTTCCG TTGCTCCTAC
    TGCAACAACA AACTTAGTCT AGGAACATAT GCATCTTTAC ATGGGAGAAT CTACTGCAAG
    CCTCACTTCA ATCAGCTCTT TAAAGCTAAA GGCAACTATG ATGAAGGCTT TGGGCACAGA
    CCACACAAGG ATCTGTGGGC AAGCAAAAAT GAAAATGAAG GGACTTTGGA AAGACCAGCC
    CAGCTTCCAA ATGTGGGGGA AACCCCTCAG AGCCCAGGGG TAGAAGATGC CCCCATTGCC
    AAGGTGGGTG TGCTGACAGC AAGTATGGAA GCCAAGGCCT CCTCTCAGCC AGAGAAAGAA
    GACAAGCCAG CAGAAACCAA GAAGCTGAAG ATCGCCTGGC CGCCCCCCAC TGAACTTGGT
    AGTTCAGGAA GTACCCTGGA GGAAGGGATC AAAGTATCCA AGCCCAAATG GCCTCCCGAG
    GATGAAATGA GCAAGCCTGA AGCTCCCGAG GATGTAGATC TGGATCTGAA GAAGCTAAGA
    CGATCATCTT CGCTGAAAGA AAGAAGCCGC CCATTCACTG TAGCAGCTTC ATTTCGAACC
    ACTTCAGTCA AGAGCCCAAA AATCTTGTCC CCACCTATAC GGAAAGGCTG GAGCATGTCA
    GAGCAGAGTG AGGAGTTTAT GGGAGGAATG ACAGCAGAAA GGAAACAAGT GGAAAATGCC
    AATACCTCCG AAAAGAATGG TAGTGTGGGA AAAACAACCC GGCAAAGCCA GGAGATTGGA
    GGAGGGGAAC CAGGGAGGAG AATCAAGGAA TATCATGGTT TTGAGATGGG GAGTGAGAAT
    CTTATAGAAA ATGGTGCGAA CGTAGATGAA GAGGATAGAA ACCTCAAACA GCAGTCTCCA
    CTAGAACCCA AGTCTACAAA TTGGTCCAGC TTTGCAGACA ATACCTCCCC TAAAGAATTC
    ACGACCCAGA ATCAGAAATC CCAGGATATG GGATTCTGGG AGGGAGAATC AGTCAAAGAG
    CTGTCTGTGG AGGAACAGAT AAAGAGAAAT CGGTACTATG ATGAGGACGA GGATGAGGAT
    GAGGAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe27011
    Clone ID Related Accession (Same CDS sequence) XM_005636851.3 , XM_005636851.2 , XM_022410910.1
    Accession Version XM_005636851.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2289bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product LIM domain and actin-binding protein 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006609.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005636851.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGAATCGA CTCCATTTAA CAGACGGCAA TGGACTTCAG TATCATTGAG GGTAACAGCC 
    AGAGAACTTT CTCTTGTCAA CAAGAATAAG TCATCGGCTA TTGTGGAAAT ATTCTCCAAG
    TACCAGAAAG CAGCTGAAGA AGCAAACATG GAAAAGAGGA GAAGTAACAC TGAAAATCTC
    CCCCAGCACT GCAGGAGGGG GATCCTGACG GTGTTAAAGA AGAAGTGGGA GAACCCAGCA
    CTGGGAACAG AGCCTCACCC AGACTCCCTG CGAAACGGCA GCACTGAGGT CAGACCCGGA
    GGCGACCATC CTCCGGGTGA AGTGGCAAGA AGCTCTGCCT CTGAGGCCGA AGCTGACCAG
    GAAGAAAGAG CCCACCCCAG ACCTAGGCAC GGATCACCTC CTGAAGCCCT AATTCAGTGT
    CCGTATCCCC GCACTGAGGG CAGTGAGCAT CTTAAAGACC CCTCGAAAGA AAGTAAAAAA
    ATGGAAACGT GTGTAGGAGA ACCCAGGCAT GAAGTAGGAA AACCTGAAAT CAGTGAAAAC
    ACAGAAGGCG CAAACAAAAT AGAGAAATAC AATGTTCCGC TGAACAGGCT TAAGATGATG
    TTTGAGAAAG GTGAACCGAC TCAAACCAAG ATTCTTCGGA CCCAGAGCCG TAGTGCAGGT
    GGGAGGAGGA TCTCAGAAAA CAGCTATTCT CTGGATGATT TGGAAATAGG CGCAGGTCAG
    GTGTCATCCT CTACATCCAA CGCAGAGAAA AGCGAGAATA GGCGAACTCT GGACTTACCA
    CGCCTGTCAG AAACCTCCAT AAAGGATCGA ATGGCCAAGT ACCAGGCAGC TGTGTCCAAA
    CAGAGCAGTT CCACCAACGC TATAAATGAC TTGAGAGCCA ATTGTGGTGA AATCAAAACT
    CATAAATTGG AGCAAAAGGA GAATGTGCCC CCAGATCCTG AGGTCTGCAT CTCCCATCAG
    GCTGGAGAAA AGGTCTCCGC AGCTGAGAAT AGCCTGGCAG TCCATTCCAC TCCTGCTGAA
    GATGACTCCC CAGGTAACTT CCAGGTCAAG AGTGAGGCCC AGCAGCCTGT CCATCCCAAG
    CCACTAAATC CAGATGCCAG AGCCTCCAGC CTTTCTGAAA GTTCTCCACC CAAACCAGTG
    AAGAAATTTC AGGCACCTGC AAGAGAGACT TGTGTGGAAT GTCAGAAGAC GGTTTACCCC
    ATGGAACGTC TCTTCGCTAA CCAGCAGGTG TTTCACATCA GCTGTTTCCG TTGCTCCTAC
    TGCAACAACA AACTTAGTCT AGGAACATAT GCATCTTTAC ATGGGAGAAT CTACTGCAAG
    CCTCACTTCA ATCAGCTCTT TAAAGCTAAA GGCAACTATG ATGAAGGCTT TGGGCACAGA
    CCACACAAGG ATCTGTGGGC AAGCAAAAAT GAAAATGAAG GGACTTTGGA AAGACCAGCC
    CAGCTTCCAA ATGTGGGGGA AACCCCTCAG AGCCCAGGGG TAGAAGATGC CCCCATTGCC
    AAGGTGGGTG TGCTGACAGC AAGTATGGAA GCCAAGGCCT CCTCTCAGCC AGAGAAAGAA
    GACAAGCCAG CAGAAACCAA GAAGCTGAAG ATCGCCTGGC CGCCCCCCAC TGAACTTGGT
    AGTTCAGGAA GTACCCTGGA GGAAGGGATC AAAGTATCCA AGCCCAAATG GCCTCCCGAG
    GATGAAATGA GCAAGCCTGA AGCTCCCGAG GATGTAGATC TGGATCTGAA GAAGCTAAGA
    CGATCATCTT CGCTGAAAGA AAGAAGCCGC CCATTCACTG TAGCAGCTTC ATTTCGAACC
    ACTTCAGTCA AGAGCCCAAA AATCTTGTCC CCACCTATAC GGAAAGGCTG GAGCATGTCA
    GAGCAGAGTG AGGAGTTTAT GGGAGGAATG ACAGCAGAAA GGAAACAAGT GGAAAATGCC
    AATACCTCCG AAAAGAATGG TAGTGTGGGA AAAACAACCC GGCAAAGCCA GGAGATTGGA
    GGAGGGGAAC CAGGGAGGAG AATCAAGGAA TATCATGGTT TTGAGATGGG GAGTGAGAAT
    CTTATAGAAA ATGGTGCGAA CGTAGATGAA GAGGATAGAA ACCTCAAACA GCAGTCTCCA
    CTAGAACCCA AGTCTACAAA TTGGTCCAGC TTTGCAGACA ATACCTCCCC TAAAGAATTC
    ACGACCCAGA ATCAGAAATC CCAGGATATG GGATTCTGGG AGGGAGAATC AGTCAAAGAG
    CTGTCTGTGG AGGAACAGAT AAAGAGAAAT CGGTACTATG ATGAGGACGA GGATGAGGAT
    GAGGAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005636908.1
    CDS153..2441
    Translation

    Target ORF information:

    RefSeq Version XM_005636851.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris LIM domain and actin binding 1 (LIMA1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005636851.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGAATCGA CTCCATTTAA CAGACGGCAA TGGACTTCAG TATCATTGAG GGTAACAGCC 
    AGAGAACTTT CTCTTGTCAA CAAGAATAAG TCATCGGCTA TTGTGGAAAT ATTCTCCAAG
    TACCAGAAAG CAGCTGAAGA AGCAAACATG GAAAAGAGGA GAAGTAACAC TGAAAATCTC
    CCCCAGCACT GCAGGAGGGG GATCCTGACG GTGTTAAAGA AGAAGTGGGA GAACCCAGCA
    CTGGGAACAG AGCCTCACCC AGACTCCCTG CGAAACGGCA GCACTGAGGT CAGACCCGGA
    GGCGACCATC CTCCGGGTGA AGTGGCAAGA AGCTCTGCCT CTGAGGCCGA AGCTGACCAG
    GAAGAAAGAG CCCACCCCAG ACCTAGGCAC GGATCACCTC CTGAAGCCCT AATTCAGTGT
    CCGTATCCCC GCACTGAGGG CAGTGAGCAT CTTAAAGACC CCTCGAAAGA AAGTAAAAAA
    ATGGAAACGT GTGTAGGAGA ACCCAGGCAT GAAGTAGGAA AACCTGAAAT CAGTGAAAAC
    ACAGAAGGCG CAAACAAAAT AGAGAAATAC AATGTTCCGC TGAACAGGCT TAAGATGATG
    TTTGAGAAAG GTGAACCGAC TCAAACCAAG ATTCTTCGGA CCCAGAGCCG TAGTGCAGGT
    GGGAGGAGGA TCTCAGAAAA CAGCTATTCT CTGGATGATT TGGAAATAGG CGCAGGTCAG
    GTGTCATCCT CTACATCCAA CGCAGAGAAA AGCGAGAATA GGCGAACTCT GGACTTACCA
    CGCCTGTCAG AAACCTCCAT AAAGGATCGA ATGGCCAAGT ACCAGGCAGC TGTGTCCAAA
    CAGAGCAGTT CCACCAACGC TATAAATGAC TTGAGAGCCA ATTGTGGTGA AATCAAAACT
    CATAAATTGG AGCAAAAGGA GAATGTGCCC CCAGATCCTG AGGTCTGCAT CTCCCATCAG
    GCTGGAGAAA AGGTCTCCGC AGCTGAGAAT AGCCTGGCAG TCCATTCCAC TCCTGCTGAA
    GATGACTCCC CAGGTAACTT CCAGGTCAAG AGTGAGGCCC AGCAGCCTGT CCATCCCAAG
    CCACTAAATC CAGATGCCAG AGCCTCCAGC CTTTCTGAAA GTTCTCCACC CAAACCAGTG
    AAGAAATTTC AGGCACCTGC AAGAGAGACT TGTGTGGAAT GTCAGAAGAC GGTTTACCCC
    ATGGAACGTC TCTTCGCTAA CCAGCAGGTG TTTCACATCA GCTGTTTCCG TTGCTCCTAC
    TGCAACAACA AACTTAGTCT AGGAACATAT GCATCTTTAC ATGGGAGAAT CTACTGCAAG
    CCTCACTTCA ATCAGCTCTT TAAAGCTAAA GGCAACTATG ATGAAGGCTT TGGGCACAGA
    CCACACAAGG ATCTGTGGGC AAGCAAAAAT GAAAATGAAG GGACTTTGGA AAGACCAGCC
    CAGCTTCCAA ATGTGGGGGA AACCCCTCAG AGCCCAGGGG TAGAAGATGC CCCCATTGCC
    AAGGTGGGTG TGCTGACAGC AAGTATGGAA GCCAAGGCCT CCTCTCAGCC AGAGAAAGAA
    GACAAGCCAG CAGAAACCAA GAAGCTGAAG ATCGCCTGGC CGCCCCCCAC TGAACTTGGT
    AGTTCAGGAA GTACCCTGGA GGAAGGGATC AAAGTATCCA AGCCCAAATG GCCTCCCGAG
    GATGAAATGA GCAAGCCTGA AGCTCCCGAG GATGTAGATC TGGATCTGAA GAAGCTAAGA
    CGATCATCTT CGCTGAAAGA AAGAAGCCGC CCATTCACTG TAGCAGCTTC ATTTCGAACC
    ACTTCAGTCA AGAGCCCAAA AATCTTGTCC CCACCTATAC GGAAAGGCTG GAGCATGTCA
    GAGCAGAGTG AGGAGTTTAT GGGAGGAATG ACAGCAGAAA GGAAACAAGT GGAAAATGCC
    AATACCTCCG AAAAGAATGG TAGTGTGGGA AAAACAACCC GGCAAAGCCA GGAGATTGGA
    GGAGGGGAAC CAGGGAGGAG AATCAAGGAA TATCATGGTT TTGAGATGGG GAGTGAGAAT
    CTTATAGAAA ATGGTGCGAA CGTAGATGAA GAGGATAGAA ACCTCAAACA GCAGTCTCCA
    CTAGAACCCA AGTCTACAAA TTGGTCCAGC TTTGCAGACA ATACCTCCCC TAAAGAATTC
    ACGACCCAGA ATCAGAAATC CCAGGATATG GGATTCTGGG AGGGAGAATC AGTCAAAGAG
    CTGTCTGTGG AGGAACAGAT AAAGAGAAAT CGGTACTATG ATGAGGACGA GGATGAGGAT
    GAGGAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe27011
    Clone ID Related Accession (Same CDS sequence) XM_005636851.3 , XM_005636851.2 , XM_022410910.1
    Accession Version XM_005636851.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2289bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product LIM domain and actin-binding protein 1 isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726108.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005636851.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGAATCGA CTCCATTTAA CAGACGGCAA TGGACTTCAG TATCATTGAG GGTAACAGCC 
    AGAGAACTTT CTCTTGTCAA CAAGAATAAG TCATCGGCTA TTGTGGAAAT ATTCTCCAAG
    TACCAGAAAG CAGCTGAAGA AGCAAACATG GAAAAGAGGA GAAGTAACAC TGAAAATCTC
    CCCCAGCACT GCAGGAGGGG GATCCTGACG GTGTTAAAGA AGAAGTGGGA GAACCCAGCA
    CTGGGAACAG AGCCTCACCC AGACTCCCTG CGAAACGGCA GCACTGAGGT CAGACCCGGA
    GGCGACCATC CTCCGGGTGA AGTGGCAAGA AGCTCTGCCT CTGAGGCCGA AGCTGACCAG
    GAAGAAAGAG CCCACCCCAG ACCTAGGCAC GGATCACCTC CTGAAGCCCT AATTCAGTGT
    CCGTATCCCC GCACTGAGGG CAGTGAGCAT CTTAAAGACC CCTCGAAAGA AAGTAAAAAA
    ATGGAAACGT GTGTAGGAGA ACCCAGGCAT GAAGTAGGAA AACCTGAAAT CAGTGAAAAC
    ACAGAAGGCG CAAACAAAAT AGAGAAATAC AATGTTCCGC TGAACAGGCT TAAGATGATG
    TTTGAGAAAG GTGAACCGAC TCAAACCAAG ATTCTTCGGA CCCAGAGCCG TAGTGCAGGT
    GGGAGGAGGA TCTCAGAAAA CAGCTATTCT CTGGATGATT TGGAAATAGG CGCAGGTCAG
    GTGTCATCCT CTACATCCAA CGCAGAGAAA AGCGAGAATA GGCGAACTCT GGACTTACCA
    CGCCTGTCAG AAACCTCCAT AAAGGATCGA ATGGCCAAGT ACCAGGCAGC TGTGTCCAAA
    CAGAGCAGTT CCACCAACGC TATAAATGAC TTGAGAGCCA ATTGTGGTGA AATCAAAACT
    CATAAATTGG AGCAAAAGGA GAATGTGCCC CCAGATCCTG AGGTCTGCAT CTCCCATCAG
    GCTGGAGAAA AGGTCTCCGC AGCTGAGAAT AGCCTGGCAG TCCATTCCAC TCCTGCTGAA
    GATGACTCCC CAGGTAACTT CCAGGTCAAG AGTGAGGCCC AGCAGCCTGT CCATCCCAAG
    CCACTAAATC CAGATGCCAG AGCCTCCAGC CTTTCTGAAA GTTCTCCACC CAAACCAGTG
    AAGAAATTTC AGGCACCTGC AAGAGAGACT TGTGTGGAAT GTCAGAAGAC GGTTTACCCC
    ATGGAACGTC TCTTCGCTAA CCAGCAGGTG TTTCACATCA GCTGTTTCCG TTGCTCCTAC
    TGCAACAACA AACTTAGTCT AGGAACATAT GCATCTTTAC ATGGGAGAAT CTACTGCAAG
    CCTCACTTCA ATCAGCTCTT TAAAGCTAAA GGCAACTATG ATGAAGGCTT TGGGCACAGA
    CCACACAAGG ATCTGTGGGC AAGCAAAAAT GAAAATGAAG GGACTTTGGA AAGACCAGCC
    CAGCTTCCAA ATGTGGGGGA AACCCCTCAG AGCCCAGGGG TAGAAGATGC CCCCATTGCC
    AAGGTGGGTG TGCTGACAGC AAGTATGGAA GCCAAGGCCT CCTCTCAGCC AGAGAAAGAA
    GACAAGCCAG CAGAAACCAA GAAGCTGAAG ATCGCCTGGC CGCCCCCCAC TGAACTTGGT
    AGTTCAGGAA GTACCCTGGA GGAAGGGATC AAAGTATCCA AGCCCAAATG GCCTCCCGAG
    GATGAAATGA GCAAGCCTGA AGCTCCCGAG GATGTAGATC TGGATCTGAA GAAGCTAAGA
    CGATCATCTT CGCTGAAAGA AAGAAGCCGC CCATTCACTG TAGCAGCTTC ATTTCGAACC
    ACTTCAGTCA AGAGCCCAAA AATCTTGTCC CCACCTATAC GGAAAGGCTG GAGCATGTCA
    GAGCAGAGTG AGGAGTTTAT GGGAGGAATG ACAGCAGAAA GGAAACAAGT GGAAAATGCC
    AATACCTCCG AAAAGAATGG TAGTGTGGGA AAAACAACCC GGCAAAGCCA GGAGATTGGA
    GGAGGGGAAC CAGGGAGGAG AATCAAGGAA TATCATGGTT TTGAGATGGG GAGTGAGAAT
    CTTATAGAAA ATGGTGCGAA CGTAGATGAA GAGGATAGAA ACCTCAAACA GCAGTCTCCA
    CTAGAACCCA AGTCTACAAA TTGGTCCAGC TTTGCAGACA ATACCTCCCC TAAAGAATTC
    ACGACCCAGA ATCAGAAATC CCAGGATATG GGATTCTGGG AGGGAGAATC AGTCAAAGAG
    CTGTCTGTGG AGGAACAGAT AAAGAGAAAT CGGTACTATG ATGAGGACGA GGATGAGGAT
    GAGGAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005636908.1
    CDS138..2426
    Translation

    Target ORF information:

    RefSeq Version XM_005636851.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris LIM domain and actin binding 1 (LIMA1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005636851.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGAATCGA CTCCATTTAA CAGACGGCAA TGGACTTCAG TATCATTGAG GGTAACAGCC 
    AGAGAACTTT CTCTTGTCAA CAAGAATAAG TCATCGGCTA TTGTGGAAAT ATTCTCCAAG
    TACCAGAAAG CAGCTGAAGA AGCAAACATG GAAAAGAGGA GAAGTAACAC TGAAAATCTC
    CCCCAGCACT GCAGGAGGGG GATCCTGACG GTGTTAAAGA AGAAGTGGGA GAACCCAGCA
    CTGGGAACAG AGCCTCACCC AGACTCCCTG CGAAACGGCA GCACTGAGGT CAGACCCGGA
    GGCGACCATC CTCCGGGTGA AGTGGCAAGA AGCTCTGCCT CTGAGGCCGA AGCTGACCAG
    GAAGAAAGAG CCCACCCCAG ACCTAGGCAC GGATCACCTC CTGAAGCCCT AATTCAGTGT
    CCGTATCCCC GCACTGAGGG CAGTGAGCAT CTTAAAGACC CCTCGAAAGA AAGTAAAAAA
    ATGGAAACGT GTGTAGGAGA ACCCAGGCAT GAAGTAGGAA AACCTGAAAT CAGTGAAAAC
    ACAGAAGGCG CAAACAAAAT AGAGAAATAC AATGTTCCGC TGAACAGGCT TAAGATGATG
    TTTGAGAAAG GTGAACCGAC TCAAACCAAG ATTCTTCGGA CCCAGAGCCG TAGTGCAGGT
    GGGAGGAGGA TCTCAGAAAA CAGCTATTCT CTGGATGATT TGGAAATAGG CGCAGGTCAG
    GTGTCATCCT CTACATCCAA CGCAGAGAAA AGCGAGAATA GGCGAACTCT GGACTTACCA
    CGCCTGTCAG AAACCTCCAT AAAGGATCGA ATGGCCAAGT ACCAGGCAGC TGTGTCCAAA
    CAGAGCAGTT CCACCAACGC TATAAATGAC TTGAGAGCCA ATTGTGGTGA AATCAAAACT
    CATAAATTGG AGCAAAAGGA GAATGTGCCC CCAGATCCTG AGGTCTGCAT CTCCCATCAG
    GCTGGAGAAA AGGTCTCCGC AGCTGAGAAT AGCCTGGCAG TCCATTCCAC TCCTGCTGAA
    GATGACTCCC CAGGTAACTT CCAGGTCAAG AGTGAGGCCC AGCAGCCTGT CCATCCCAAG
    CCACTAAATC CAGATGCCAG AGCCTCCAGC CTTTCTGAAA GTTCTCCACC CAAACCAGTG
    AAGAAATTTC AGGCACCTGC AAGAGAGACT TGTGTGGAAT GTCAGAAGAC GGTTTACCCC
    ATGGAACGTC TCTTCGCTAA CCAGCAGGTG TTTCACATCA GCTGTTTCCG TTGCTCCTAC
    TGCAACAACA AACTTAGTCT AGGAACATAT GCATCTTTAC ATGGGAGAAT CTACTGCAAG
    CCTCACTTCA ATCAGCTCTT TAAAGCTAAA GGCAACTATG ATGAAGGCTT TGGGCACAGA
    CCACACAAGG ATCTGTGGGC AAGCAAAAAT GAAAATGAAG GGACTTTGGA AAGACCAGCC
    CAGCTTCCAA ATGTGGGGGA AACCCCTCAG AGCCCAGGGG TAGAAGATGC CCCCATTGCC
    AAGGTGGGTG TGCTGACAGC AAGTATGGAA GCCAAGGCCT CCTCTCAGCC AGAGAAAGAA
    GACAAGCCAG CAGAAACCAA GAAGCTGAAG ATCGCCTGGC CGCCCCCCAC TGAACTTGGT
    AGTTCAGGAA GTACCCTGGA GGAAGGGATC AAAGTATCCA AGCCCAAATG GCCTCCCGAG
    GATGAAATGA GCAAGCCTGA AGCTCCCGAG GATGTAGATC TGGATCTGAA GAAGCTAAGA
    CGATCATCTT CGCTGAAAGA AAGAAGCCGC CCATTCACTG TAGCAGCTTC ATTTCGAACC
    ACTTCAGTCA AGAGCCCAAA AATCTTGTCC CCACCTATAC GGAAAGGCTG GAGCATGTCA
    GAGCAGAGTG AGGAGTTTAT GGGAGGAATG ACAGCAGAAA GGAAACAAGT GGAAAATGCC
    AATACCTCCG AAAAGAATGG TAGTGTGGGA AAAACAACCC GGCAAAGCCA GGAGATTGGA
    GGAGGGGAAC CAGGGAGGAG AATCAAGGAA TATCATGGTT TTGAGATGGG GAGTGAGAAT
    CTTATAGAAA ATGGTGCGAA CGTAGATGAA GAGGATAGAA ACCTCAAACA GCAGTCTCCA
    CTAGAACCCA AGTCTACAAA TTGGTCCAGC TTTGCAGACA ATACCTCCCC TAAAGAATTC
    ACGACCCAGA ATCAGAAATC CCAGGATATG GGATTCTGGG AGGGAGAATC AGTCAAAGAG
    CTGTCTGTGG AGGAACAGAT AAAGAGAAAT CGGTACTATG ATGAGGACGA GGATGAGGAT
    GAGGAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe27011
    Clone ID Related Accession (Same CDS sequence) XM_005636851.3 , XM_005636851.2 , XM_022410910.1
    Accession Version XM_022410910.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2289bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product LIM domain and actin-binding protein 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006609.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGAATCGA CTCCATTTAA CAGACGGCAA TGGACTTCAG TATCATTGAG GGTAACAGCC 
    AGAGAACTTT CTCTTGTCAA CAAGAATAAG TCATCGGCTA TTGTGGAAAT ATTCTCCAAG
    TACCAGAAAG CAGCTGAAGA AGCAAACATG GAAAAGAGGA GAAGTAACAC TGAAAATCTC
    CCCCAGCACT GCAGGAGGGG GATCCTGACG GTGTTAAAGA AGAAGTGGGA GAACCCAGCA
    CTGGGAACAG AGCCTCACCC AGACTCCCTG CGAAACGGCA GCACTGAGGT CAGACCCGGA
    GGCGACCATC CTCCGGGTGA AGTGGCAAGA AGCTCTGCCT CTGAGGCCGA AGCTGACCAG
    GAAGAAAGAG CCCACCCCAG ACCTAGGCAC GGATCACCTC CTGAAGCCCT AATTCAGTGT
    CCGTATCCCC GCACTGAGGG CAGTGAGCAT CTTAAAGACC CCTCGAAAGA AAGTAAAAAA
    ATGGAAACGT GTGTAGGAGA ACCCAGGCAT GAAGTAGGAA AACCTGAAAT CAGTGAAAAC
    ACAGAAGGCG CAAACAAAAT AGAGAAATAC AATGTTCCGC TGAACAGGCT TAAGATGATG
    TTTGAGAAAG GTGAACCGAC TCAAACCAAG ATTCTTCGGA CCCAGAGCCG TAGTGCAGGT
    GGGAGGAGGA TCTCAGAAAA CAGCTATTCT CTGGATGATT TGGAAATAGG CGCAGGTCAG
    GTGTCATCCT CTACATCCAA CGCAGAGAAA AGCGAGAATA GGCGAACTCT GGACTTACCA
    CGCCTGTCAG AAACCTCCAT AAAGGATCGA ATGGCCAAGT ACCAGGCAGC TGTGTCCAAA
    CAGAGCAGTT CCACCAACGC TATAAATGAC TTGAGAGCCA ATTGTGGTGA AATCAAAACT
    CATAAATTGG AGCAAAAGGA GAATGTGCCC CCAGATCCTG AGGTCTGCAT CTCCCATCAG
    GCTGGAGAAA AGGTCTCCGC AGCTGAGAAT AGCCTGGCAG TCCATTCCAC TCCTGCTGAA
    GATGACTCCC CAGGTAACTT CCAGGTCAAG AGTGAGGCCC AGCAGCCTGT CCATCCCAAG
    CCACTAAATC CAGATGCCAG AGCCTCCAGC CTTTCTGAAA GTTCTCCACC CAAACCAGTG
    AAGAAATTTC AGGCACCTGC AAGAGAGACT TGTGTGGAAT GTCAGAAGAC GGTTTACCCC
    ATGGAACGTC TCTTCGCTAA CCAGCAGGTG TTTCACATCA GCTGTTTCCG TTGCTCCTAC
    TGCAACAACA AACTTAGTCT AGGAACATAT GCATCTTTAC ATGGGAGAAT CTACTGCAAG
    CCTCACTTCA ATCAGCTCTT TAAAGCTAAA GGCAACTATG ATGAAGGCTT TGGGCACAGA
    CCACACAAGG ATCTGTGGGC AAGCAAAAAT GAAAATGAAG GGACTTTGGA AAGACCAGCC
    CAGCTTCCAA ATGTGGGGGA AACCCCTCAG AGCCCAGGGG TAGAAGATGC CCCCATTGCC
    AAGGTGGGTG TGCTGACAGC AAGTATGGAA GCCAAGGCCT CCTCTCAGCC AGAGAAAGAA
    GACAAGCCAG CAGAAACCAA GAAGCTGAAG ATCGCCTGGC CGCCCCCCAC TGAACTTGGT
    AGTTCAGGAA GTACCCTGGA GGAAGGGATC AAAGTATCCA AGCCCAAATG GCCTCCCGAG
    GATGAAATGA GCAAGCCTGA AGCTCCCGAG GATGTAGATC TGGATCTGAA GAAGCTAAGA
    CGATCATCTT CGCTGAAAGA AAGAAGCCGC CCATTCACTG TAGCAGCTTC ATTTCGAACC
    ACTTCAGTCA AGAGCCCAAA AATCTTGTCC CCACCTATAC GGAAAGGCTG GAGCATGTCA
    GAGCAGAGTG AGGAGTTTAT GGGAGGAATG ACAGCAGAAA GGAAACAAGT GGAAAATGCC
    AATACCTCCG AAAAGAATGG TAGTGTGGGA AAAACAACCC GGCAAAGCCA GGAGATTGGA
    GGAGGGGAAC CAGGGAGGAG AATCAAGGAA TATCATGGTT TTGAGATGGG GAGTGAGAAT
    CTTATAGAAA ATGGTGCGAA CGTAGATGAA GAGGATAGAA ACCTCAAACA GCAGTCTCCA
    CTAGAACCCA AGTCTACAAA TTGGTCCAGC TTTGCAGACA ATACCTCCCC TAAAGAATTC
    ACGACCCAGA ATCAGAAATC CCAGGATATG GGATTCTGGG AGGGAGAATC AGTCAAAGAG
    CTGTCTGTGG AGGAACAGAT AAAGAGAAAT CGGTACTATG ATGAGGACGA GGATGAGGAT
    GAGGAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022266618.1
    CDS42..2330
    Translation

    Target ORF information:

    RefSeq Version XM_022410910.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris LIM domain and actin binding 1 (LIMA1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022410910.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGAATCGA CTCCATTTAA CAGACGGCAA TGGACTTCAG TATCATTGAG GGTAACAGCC 
    AGAGAACTTT CTCTTGTCAA CAAGAATAAG TCATCGGCTA TTGTGGAAAT ATTCTCCAAG
    TACCAGAAAG CAGCTGAAGA AGCAAACATG GAAAAGAGGA GAAGTAACAC TGAAAATCTC
    CCCCAGCACT GCAGGAGGGG GATCCTGACG GTGTTAAAGA AGAAGTGGGA GAACCCAGCA
    CTGGGAACAG AGCCTCACCC AGACTCCCTG CGAAACGGCA GCACTGAGGT CAGACCCGGA
    GGCGACCATC CTCCGGGTGA AGTGGCAAGA AGCTCTGCCT CTGAGGCCGA AGCTGACCAG
    GAAGAAAGAG CCCACCCCAG ACCTAGGCAC GGATCACCTC CTGAAGCCCT AATTCAGTGT
    CCGTATCCCC GCACTGAGGG CAGTGAGCAT CTTAAAGACC CCTCGAAAGA AAGTAAAAAA
    ATGGAAACGT GTGTAGGAGA ACCCAGGCAT GAAGTAGGAA AACCTGAAAT CAGTGAAAAC
    ACAGAAGGCG CAAACAAAAT AGAGAAATAC AATGTTCCGC TGAACAGGCT TAAGATGATG
    TTTGAGAAAG GTGAACCGAC TCAAACCAAG ATTCTTCGGA CCCAGAGCCG TAGTGCAGGT
    GGGAGGAGGA TCTCAGAAAA CAGCTATTCT CTGGATGATT TGGAAATAGG CGCAGGTCAG
    GTGTCATCCT CTACATCCAA CGCAGAGAAA AGCGAGAATA GGCGAACTCT GGACTTACCA
    CGCCTGTCAG AAACCTCCAT AAAGGATCGA ATGGCCAAGT ACCAGGCAGC TGTGTCCAAA
    CAGAGCAGTT CCACCAACGC TATAAATGAC TTGAGAGCCA ATTGTGGTGA AATCAAAACT
    CATAAATTGG AGCAAAAGGA GAATGTGCCC CCAGATCCTG AGGTCTGCAT CTCCCATCAG
    GCTGGAGAAA AGGTCTCCGC AGCTGAGAAT AGCCTGGCAG TCCATTCCAC TCCTGCTGAA
    GATGACTCCC CAGGTAACTT CCAGGTCAAG AGTGAGGCCC AGCAGCCTGT CCATCCCAAG
    CCACTAAATC CAGATGCCAG AGCCTCCAGC CTTTCTGAAA GTTCTCCACC CAAACCAGTG
    AAGAAATTTC AGGCACCTGC AAGAGAGACT TGTGTGGAAT GTCAGAAGAC GGTTTACCCC
    ATGGAACGTC TCTTCGCTAA CCAGCAGGTG TTTCACATCA GCTGTTTCCG TTGCTCCTAC
    TGCAACAACA AACTTAGTCT AGGAACATAT GCATCTTTAC ATGGGAGAAT CTACTGCAAG
    CCTCACTTCA ATCAGCTCTT TAAAGCTAAA GGCAACTATG ATGAAGGCTT TGGGCACAGA
    CCACACAAGG ATCTGTGGGC AAGCAAAAAT GAAAATGAAG GGACTTTGGA AAGACCAGCC
    CAGCTTCCAA ATGTGGGGGA AACCCCTCAG AGCCCAGGGG TAGAAGATGC CCCCATTGCC
    AAGGTGGGTG TGCTGACAGC AAGTATGGAA GCCAAGGCCT CCTCTCAGCC AGAGAAAGAA
    GACAAGCCAG CAGAAACCAA GAAGCTGAAG ATCGCCTGGC CGCCCCCCAC TGAACTTGGT
    AGTTCAGGAA GTACCCTGGA GGAAGGGATC AAAGTATCCA AGCCCAAATG GCCTCCCGAG
    GATGAAATGA GCAAGCCTGA AGCTCCCGAG GATGTAGATC TGGATCTGAA GAAGCTAAGA
    CGATCATCTT CGCTGAAAGA AAGAAGCCGC CCATTCACTG TAGCAGCTTC ATTTCGAACC
    ACTTCAGTCA AGAGCCCAAA AATCTTGTCC CCACCTATAC GGAAAGGCTG GAGCATGTCA
    GAGCAGAGTG AGGAGTTTAT GGGAGGAATG ACAGCAGAAA GGAAACAAGT GGAAAATGCC
    AATACCTCCG AAAAGAATGG TAGTGTGGGA AAAACAACCC GGCAAAGCCA GGAGATTGGA
    GGAGGGGAAC CAGGGAGGAG AATCAAGGAA TATCATGGTT TTGAGATGGG GAGTGAGAAT
    CTTATAGAAA ATGGTGCGAA CGTAGATGAA GAGGATAGAA ACCTCAAACA GCAGTCTCCA
    CTAGAACCCA AGTCTACAAA TTGGTCCAGC TTTGCAGACA ATACCTCCCC TAAAGAATTC
    ACGACCCAGA ATCAGAAATC CCAGGATATG GGATTCTGGG AGGGAGAATC AGTCAAAGAG
    CTGTCTGTGG AGGAACAGAT AAAGAGAAAT CGGTACTATG ATGAGGACGA GGATGAGGAT
    GAGGAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe27012
    Clone ID Related Accession (Same CDS sequence) XM_534804.5 , XM_534804.6
    Accession Version XM_534804.5 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2286bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product LIM domain and actin-binding protein 1 isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726108.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_534804.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGAATCGA CTCCATTTAA CAGACGGCAA TGGACTTCAG TATCATTGAG GGTAACAGCC 
    AGAGAACTTT CTCTTGTCAA CAAGAATAAG TCATCGGCTA TTGTGGAAAT ATTCTCCAAG
    TACCAGAAAG CAGCTGAAGA AGCAAACATG GAAAAGAGGA GAAGTAACAC TGAAAATCTC
    CCCCAGCACT GCAGGAGGGG GATCCTGACG GTGTTAAAGA AGAAGTGGGA GAACCCAGCA
    CTGGGAACAG AGCCTCACCC AGACTCCCTG CGAAACGGCA GCACTGAGGT CAGACCCGGA
    GGCGACCATC CTCCGGGTGA AGTGGCAAGA AGCTCTGCCT CTGAGGCCGA AGCTGACCAG
    GAAGAAAGAG CCCACCCCAG ACCTAGGCAC GGATCACCTC CTGAAGCCCT AATTCAGTGT
    CCGTATCCCC GCACTGAGGG CAGTGAGCAT CTTAAAGACC CCTCGAAAGA AAGTAAAAAA
    ATGGAAACGT GTGTAGGAGA ACCCAGGCAT GAAGTAGGAA AACCTGAAAT CAGTGAAAAC
    ACAGAAGGCG CAAACAAAAT AGAGAAATAC AATGTTCCGC TGAACAGGCT TAAGATGATG
    TTTGAGAAAG GTGAACCGAC TCAAACCAAG ATTCTTCGGA CCCAGAGCCG TAGTGCAGGT
    GGGAGGAGGA TCTCAGAAAA CAGCTATTCT CTGGATGATT TGGAAATAGG CGCAGGTCAG
    GTGTCATCCT CTACATCCAA CGCAGAGAAA AGCGAGAATA GGCGAACTCT GGACTTACCA
    CGCCTGTCAG AAACCTCCAT AAAGGATCGA ATGGCCAAGT ACCAGGCAGC TGTGTCCAAA
    CAGAGCAGTT CCACCAACGC TATAAATGAC TTGAGAGCCA ATTGTGGTGA AATCAAAACT
    CATAAATTGG AGCAAAAGGA GAATGTGCCC CCAGATCCTG AGGTCTGCAT CTCCCATCAG
    GCTGGAGAAA AGGTCTCCGC AGCTGAGAAT AGCCTGGCAG TCCATTCCAC TCCTGCTGAA
    GATGACTCCC GTAACTTCCA GGTCAAGAGT GAGGCCCAGC AGCCTGTCCA TCCCAAGCCA
    CTAAATCCAG ATGCCAGAGC CTCCAGCCTT TCTGAAAGTT CTCCACCCAA ACCAGTGAAG
    AAATTTCAGG CACCTGCAAG AGAGACTTGT GTGGAATGTC AGAAGACGGT TTACCCCATG
    GAACGTCTCT TCGCTAACCA GCAGGTGTTT CACATCAGCT GTTTCCGTTG CTCCTACTGC
    AACAACAAAC TTAGTCTAGG AACATATGCA TCTTTACATG GGAGAATCTA CTGCAAGCCT
    CACTTCAATC AGCTCTTTAA AGCTAAAGGC AACTATGATG AAGGCTTTGG GCACAGACCA
    CACAAGGATC TGTGGGCAAG CAAAAATGAA AATGAAGGGA CTTTGGAAAG ACCAGCCCAG
    CTTCCAAATG TGGGGGAAAC CCCTCAGAGC CCAGGGGTAG AAGATGCCCC CATTGCCAAG
    GTGGGTGTGC TGACAGCAAG TATGGAAGCC AAGGCCTCCT CTCAGCCAGA GAAAGAAGAC
    AAGCCAGCAG AAACCAAGAA GCTGAAGATC GCCTGGCCGC CCCCCACTGA ACTTGGTAGT
    TCAGGAAGTA CCCTGGAGGA AGGGATCAAA GTATCCAAGC CCAAATGGCC TCCCGAGGAT
    GAAATGAGCA AGCCTGAAGC TCCCGAGGAT GTAGATCTGG ATCTGAAGAA GCTAAGACGA
    TCATCTTCGC TGAAAGAAAG AAGCCGCCCA TTCACTGTAG CAGCTTCATT TCGAACCACT
    TCAGTCAAGA GCCCAAAAAT CTTGTCCCCA CCTATACGGA AAGGCTGGAG CATGTCAGAG
    CAGAGTGAGG AGTTTATGGG AGGAATGACA GCAGAAAGGA AACAAGTGGA AAATGCCAAT
    ACCTCCGAAA AGAATGGTAG TGTGGGAAAA ACAACCCGGC AAAGCCAGGA GATTGGAGGA
    GGGGAACCAG GGAGGAGAAT CAAGGAATAT CATGGTTTTG AGATGGGGAG TGAGAATCTT
    ATAGAAAATG GTGCGAACGT AGATGAAGAG GATAGAAACC TCAAACAGCA GTCTCCACTA
    GAACCCAAGT CTACAAATTG GTCCAGCTTT GCAGACAATA CCTCCCCTAA AGAATTCACG
    ACCCAGAATC AGAAATCCCA GGATATGGGA TTCTGGGAGG GAGAATCAGT CAAAGAGCTG
    TCTGTGGAGG AACAGATAAA GAGAAATCGG TACTATGATG AGGACGAGGA TGAGGATGAG
    GAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_534804.2
    CDS138..2423
    Translation

    Target ORF information:

    RefSeq Version XM_534804.5
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris LIM domain and actin binding 1 (LIMA1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_534804.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGAATCGA CTCCATTTAA CAGACGGCAA TGGACTTCAG TATCATTGAG GGTAACAGCC 
    AGAGAACTTT CTCTTGTCAA CAAGAATAAG TCATCGGCTA TTGTGGAAAT ATTCTCCAAG
    TACCAGAAAG CAGCTGAAGA AGCAAACATG GAAAAGAGGA GAAGTAACAC TGAAAATCTC
    CCCCAGCACT GCAGGAGGGG GATCCTGACG GTGTTAAAGA AGAAGTGGGA GAACCCAGCA
    CTGGGAACAG AGCCTCACCC AGACTCCCTG CGAAACGGCA GCACTGAGGT CAGACCCGGA
    GGCGACCATC CTCCGGGTGA AGTGGCAAGA AGCTCTGCCT CTGAGGCCGA AGCTGACCAG
    GAAGAAAGAG CCCACCCCAG ACCTAGGCAC GGATCACCTC CTGAAGCCCT AATTCAGTGT
    CCGTATCCCC GCACTGAGGG CAGTGAGCAT CTTAAAGACC CCTCGAAAGA AAGTAAAAAA
    ATGGAAACGT GTGTAGGAGA ACCCAGGCAT GAAGTAGGAA AACCTGAAAT CAGTGAAAAC
    ACAGAAGGCG CAAACAAAAT AGAGAAATAC AATGTTCCGC TGAACAGGCT TAAGATGATG
    TTTGAGAAAG GTGAACCGAC TCAAACCAAG ATTCTTCGGA CCCAGAGCCG TAGTGCAGGT
    GGGAGGAGGA TCTCAGAAAA CAGCTATTCT CTGGATGATT TGGAAATAGG CGCAGGTCAG
    GTGTCATCCT CTACATCCAA CGCAGAGAAA AGCGAGAATA GGCGAACTCT GGACTTACCA
    CGCCTGTCAG AAACCTCCAT AAAGGATCGA ATGGCCAAGT ACCAGGCAGC TGTGTCCAAA
    CAGAGCAGTT CCACCAACGC TATAAATGAC TTGAGAGCCA ATTGTGGTGA AATCAAAACT
    CATAAATTGG AGCAAAAGGA GAATGTGCCC CCAGATCCTG AGGTCTGCAT CTCCCATCAG
    GCTGGAGAAA AGGTCTCCGC AGCTGAGAAT AGCCTGGCAG TCCATTCCAC TCCTGCTGAA
    GATGACTCCC GTAACTTCCA GGTCAAGAGT GAGGCCCAGC AGCCTGTCCA TCCCAAGCCA
    CTAAATCCAG ATGCCAGAGC CTCCAGCCTT TCTGAAAGTT CTCCACCCAA ACCAGTGAAG
    AAATTTCAGG CACCTGCAAG AGAGACTTGT GTGGAATGTC AGAAGACGGT TTACCCCATG
    GAACGTCTCT TCGCTAACCA GCAGGTGTTT CACATCAGCT GTTTCCGTTG CTCCTACTGC
    AACAACAAAC TTAGTCTAGG AACATATGCA TCTTTACATG GGAGAATCTA CTGCAAGCCT
    CACTTCAATC AGCTCTTTAA AGCTAAAGGC AACTATGATG AAGGCTTTGG GCACAGACCA
    CACAAGGATC TGTGGGCAAG CAAAAATGAA AATGAAGGGA CTTTGGAAAG ACCAGCCCAG
    CTTCCAAATG TGGGGGAAAC CCCTCAGAGC CCAGGGGTAG AAGATGCCCC CATTGCCAAG
    GTGGGTGTGC TGACAGCAAG TATGGAAGCC AAGGCCTCCT CTCAGCCAGA GAAAGAAGAC
    AAGCCAGCAG AAACCAAGAA GCTGAAGATC GCCTGGCCGC CCCCCACTGA ACTTGGTAGT
    TCAGGAAGTA CCCTGGAGGA AGGGATCAAA GTATCCAAGC CCAAATGGCC TCCCGAGGAT
    GAAATGAGCA AGCCTGAAGC TCCCGAGGAT GTAGATCTGG ATCTGAAGAA GCTAAGACGA
    TCATCTTCGC TGAAAGAAAG AAGCCGCCCA TTCACTGTAG CAGCTTCATT TCGAACCACT
    TCAGTCAAGA GCCCAAAAAT CTTGTCCCCA CCTATACGGA AAGGCTGGAG CATGTCAGAG
    CAGAGTGAGG AGTTTATGGG AGGAATGACA GCAGAAAGGA AACAAGTGGA AAATGCCAAT
    ACCTCCGAAA AGAATGGTAG TGTGGGAAAA ACAACCCGGC AAAGCCAGGA GATTGGAGGA
    GGGGAACCAG GGAGGAGAAT CAAGGAATAT CATGGTTTTG AGATGGGGAG TGAGAATCTT
    ATAGAAAATG GTGCGAACGT AGATGAAGAG GATAGAAACC TCAAACAGCA GTCTCCACTA
    GAACCCAAGT CTACAAATTG GTCCAGCTTT GCAGACAATA CCTCCCCTAA AGAATTCACG
    ACCCAGAATC AGAAATCCCA GGATATGGGA TTCTGGGAGG GAGAATCAGT CAAAGAGCTG
    TCTGTGGAGG AACAGATAAA GAGAAATCGG TACTATGATG AGGACGAGGA TGAGGATGAG
    GAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe27012
    Clone ID Related Accession (Same CDS sequence) XM_534804.5 , XM_534804.6
    Accession Version XM_534804.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2286bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product LIM domain and actin-binding protein 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006609.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_534804.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGAATCGA CTCCATTTAA CAGACGGCAA TGGACTTCAG TATCATTGAG GGTAACAGCC 
    AGAGAACTTT CTCTTGTCAA CAAGAATAAG TCATCGGCTA TTGTGGAAAT ATTCTCCAAG
    TACCAGAAAG CAGCTGAAGA AGCAAACATG GAAAAGAGGA GAAGTAACAC TGAAAATCTC
    CCCCAGCACT GCAGGAGGGG GATCCTGACG GTGTTAAAGA AGAAGTGGGA GAACCCAGCA
    CTGGGAACAG AGCCTCACCC AGACTCCCTG CGAAACGGCA GCACTGAGGT CAGACCCGGA
    GGCGACCATC CTCCGGGTGA AGTGGCAAGA AGCTCTGCCT CTGAGGCCGA AGCTGACCAG
    GAAGAAAGAG CCCACCCCAG ACCTAGGCAC GGATCACCTC CTGAAGCCCT AATTCAGTGT
    CCGTATCCCC GCACTGAGGG CAGTGAGCAT CTTAAAGACC CCTCGAAAGA AAGTAAAAAA
    ATGGAAACGT GTGTAGGAGA ACCCAGGCAT GAAGTAGGAA AACCTGAAAT CAGTGAAAAC
    ACAGAAGGCG CAAACAAAAT AGAGAAATAC AATGTTCCGC TGAACAGGCT TAAGATGATG
    TTTGAGAAAG GTGAACCGAC TCAAACCAAG ATTCTTCGGA CCCAGAGCCG TAGTGCAGGT
    GGGAGGAGGA TCTCAGAAAA CAGCTATTCT CTGGATGATT TGGAAATAGG CGCAGGTCAG
    GTGTCATCCT CTACATCCAA CGCAGAGAAA AGCGAGAATA GGCGAACTCT GGACTTACCA
    CGCCTGTCAG AAACCTCCAT AAAGGATCGA ATGGCCAAGT ACCAGGCAGC TGTGTCCAAA
    CAGAGCAGTT CCACCAACGC TATAAATGAC TTGAGAGCCA ATTGTGGTGA AATCAAAACT
    CATAAATTGG AGCAAAAGGA GAATGTGCCC CCAGATCCTG AGGTCTGCAT CTCCCATCAG
    GCTGGAGAAA AGGTCTCCGC AGCTGAGAAT AGCCTGGCAG TCCATTCCAC TCCTGCTGAA
    GATGACTCCC GTAACTTCCA GGTCAAGAGT GAGGCCCAGC AGCCTGTCCA TCCCAAGCCA
    CTAAATCCAG ATGCCAGAGC CTCCAGCCTT TCTGAAAGTT CTCCACCCAA ACCAGTGAAG
    AAATTTCAGG CACCTGCAAG AGAGACTTGT GTGGAATGTC AGAAGACGGT TTACCCCATG
    GAACGTCTCT TCGCTAACCA GCAGGTGTTT CACATCAGCT GTTTCCGTTG CTCCTACTGC
    AACAACAAAC TTAGTCTAGG AACATATGCA TCTTTACATG GGAGAATCTA CTGCAAGCCT
    CACTTCAATC AGCTCTTTAA AGCTAAAGGC AACTATGATG AAGGCTTTGG GCACAGACCA
    CACAAGGATC TGTGGGCAAG CAAAAATGAA AATGAAGGGA CTTTGGAAAG ACCAGCCCAG
    CTTCCAAATG TGGGGGAAAC CCCTCAGAGC CCAGGGGTAG AAGATGCCCC CATTGCCAAG
    GTGGGTGTGC TGACAGCAAG TATGGAAGCC AAGGCCTCCT CTCAGCCAGA GAAAGAAGAC
    AAGCCAGCAG AAACCAAGAA GCTGAAGATC GCCTGGCCGC CCCCCACTGA ACTTGGTAGT
    TCAGGAAGTA CCCTGGAGGA AGGGATCAAA GTATCCAAGC CCAAATGGCC TCCCGAGGAT
    GAAATGAGCA AGCCTGAAGC TCCCGAGGAT GTAGATCTGG ATCTGAAGAA GCTAAGACGA
    TCATCTTCGC TGAAAGAAAG AAGCCGCCCA TTCACTGTAG CAGCTTCATT TCGAACCACT
    TCAGTCAAGA GCCCAAAAAT CTTGTCCCCA CCTATACGGA AAGGCTGGAG CATGTCAGAG
    CAGAGTGAGG AGTTTATGGG AGGAATGACA GCAGAAAGGA AACAAGTGGA AAATGCCAAT
    ACCTCCGAAA AGAATGGTAG TGTGGGAAAA ACAACCCGGC AAAGCCAGGA GATTGGAGGA
    GGGGAACCAG GGAGGAGAAT CAAGGAATAT CATGGTTTTG AGATGGGGAG TGAGAATCTT
    ATAGAAAATG GTGCGAACGT AGATGAAGAG GATAGAAACC TCAAACAGCA GTCTCCACTA
    GAACCCAAGT CTACAAATTG GTCCAGCTTT GCAGACAATA CCTCCCCTAA AGAATTCACG
    ACCCAGAATC AGAAATCCCA GGATATGGGA TTCTGGGAGG GAGAATCAGT CAAAGAGCTG
    TCTGTGGAGG AACAGATAAA GAGAAATCGG TACTATGATG AGGACGAGGA TGAGGATGAG
    GAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_534804.2
    CDS153..2438
    Translation

    Target ORF information:

    RefSeq Version XM_534804.6
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris LIM domain and actin binding 1 (LIMA1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_534804.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGAATCGA CTCCATTTAA CAGACGGCAA TGGACTTCAG TATCATTGAG GGTAACAGCC 
    AGAGAACTTT CTCTTGTCAA CAAGAATAAG TCATCGGCTA TTGTGGAAAT ATTCTCCAAG
    TACCAGAAAG CAGCTGAAGA AGCAAACATG GAAAAGAGGA GAAGTAACAC TGAAAATCTC
    CCCCAGCACT GCAGGAGGGG GATCCTGACG GTGTTAAAGA AGAAGTGGGA GAACCCAGCA
    CTGGGAACAG AGCCTCACCC AGACTCCCTG CGAAACGGCA GCACTGAGGT CAGACCCGGA
    GGCGACCATC CTCCGGGTGA AGTGGCAAGA AGCTCTGCCT CTGAGGCCGA AGCTGACCAG
    GAAGAAAGAG CCCACCCCAG ACCTAGGCAC GGATCACCTC CTGAAGCCCT AATTCAGTGT
    CCGTATCCCC GCACTGAGGG CAGTGAGCAT CTTAAAGACC CCTCGAAAGA AAGTAAAAAA
    ATGGAAACGT GTGTAGGAGA ACCCAGGCAT GAAGTAGGAA AACCTGAAAT CAGTGAAAAC
    ACAGAAGGCG CAAACAAAAT AGAGAAATAC AATGTTCCGC TGAACAGGCT TAAGATGATG
    TTTGAGAAAG GTGAACCGAC TCAAACCAAG ATTCTTCGGA CCCAGAGCCG TAGTGCAGGT
    GGGAGGAGGA TCTCAGAAAA CAGCTATTCT CTGGATGATT TGGAAATAGG CGCAGGTCAG
    GTGTCATCCT CTACATCCAA CGCAGAGAAA AGCGAGAATA GGCGAACTCT GGACTTACCA
    CGCCTGTCAG AAACCTCCAT AAAGGATCGA ATGGCCAAGT ACCAGGCAGC TGTGTCCAAA
    CAGAGCAGTT CCACCAACGC TATAAATGAC TTGAGAGCCA ATTGTGGTGA AATCAAAACT
    CATAAATTGG AGCAAAAGGA GAATGTGCCC CCAGATCCTG AGGTCTGCAT CTCCCATCAG
    GCTGGAGAAA AGGTCTCCGC AGCTGAGAAT AGCCTGGCAG TCCATTCCAC TCCTGCTGAA
    GATGACTCCC GTAACTTCCA GGTCAAGAGT GAGGCCCAGC AGCCTGTCCA TCCCAAGCCA
    CTAAATCCAG ATGCCAGAGC CTCCAGCCTT TCTGAAAGTT CTCCACCCAA ACCAGTGAAG
    AAATTTCAGG CACCTGCAAG AGAGACTTGT GTGGAATGTC AGAAGACGGT TTACCCCATG
    GAACGTCTCT TCGCTAACCA GCAGGTGTTT CACATCAGCT GTTTCCGTTG CTCCTACTGC
    AACAACAAAC TTAGTCTAGG AACATATGCA TCTTTACATG GGAGAATCTA CTGCAAGCCT
    CACTTCAATC AGCTCTTTAA AGCTAAAGGC AACTATGATG AAGGCTTTGG GCACAGACCA
    CACAAGGATC TGTGGGCAAG CAAAAATGAA AATGAAGGGA CTTTGGAAAG ACCAGCCCAG
    CTTCCAAATG TGGGGGAAAC CCCTCAGAGC CCAGGGGTAG AAGATGCCCC CATTGCCAAG
    GTGGGTGTGC TGACAGCAAG TATGGAAGCC AAGGCCTCCT CTCAGCCAGA GAAAGAAGAC
    AAGCCAGCAG AAACCAAGAA GCTGAAGATC GCCTGGCCGC CCCCCACTGA ACTTGGTAGT
    TCAGGAAGTA CCCTGGAGGA AGGGATCAAA GTATCCAAGC CCAAATGGCC TCCCGAGGAT
    GAAATGAGCA AGCCTGAAGC TCCCGAGGAT GTAGATCTGG ATCTGAAGAA GCTAAGACGA
    TCATCTTCGC TGAAAGAAAG AAGCCGCCCA TTCACTGTAG CAGCTTCATT TCGAACCACT
    TCAGTCAAGA GCCCAAAAAT CTTGTCCCCA CCTATACGGA AAGGCTGGAG CATGTCAGAG
    CAGAGTGAGG AGTTTATGGG AGGAATGACA GCAGAAAGGA AACAAGTGGA AAATGCCAAT
    ACCTCCGAAA AGAATGGTAG TGTGGGAAAA ACAACCCGGC AAAGCCAGGA GATTGGAGGA
    GGGGAACCAG GGAGGAGAAT CAAGGAATAT CATGGTTTTG AGATGGGGAG TGAGAATCTT
    ATAGAAAATG GTGCGAACGT AGATGAAGAG GATAGAAACC TCAAACAGCA GTCTCCACTA
    GAACCCAAGT CTACAAATTG GTCCAGCTTT GCAGACAATA CCTCCCCTAA AGAATTCACG
    ACCCAGAATC AGAAATCCCA GGATATGGGA TTCTGGGAGG GAGAATCAGT CAAAGAGCTG
    TCTGTGGAGG AACAGATAAA GAGAAATCGG TACTATGATG AGGACGAGGA TGAGGATGAG
    GAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.