LIMA1 cDNA ORF clone, Bos taurus(cattle)

The following LIMA1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LIMA1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa234554 XM_024992054.1
Latest version!
Bos taurus LIM domain and actin binding 1 (LIMA1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $223.30
$319.00
OBa37169 NM_001192754.1
Latest version!
Bos taurus LIM domain and actin binding 1 (LIMA1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa19234 XM_015470991.2
Latest version!
Bos taurus LIM domain and actin binding 1 (LIMA1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa19234 XM_005206324.4
Latest version!
Bos taurus LIM domain and actin binding 1 (LIMA1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa19235 XM_005206325.4
Latest version!
Bos taurus LIM domain and actin binding 1 (LIMA1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa54749 XM_010804991.2
Latest version!
Bos taurus LIM domain and actin binding 1 (LIMA1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
View Old Accession Versions >>
OBa19234 XM_015470991.1 Bos taurus LIM domain and actin binding 1 (LIMA1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa19235 XM_005206325.3 Bos taurus LIM domain and actin binding 1 (LIMA1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa19234 XM_005206324.3 Bos taurus LIM domain and actin binding 1 (LIMA1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa234554
    Clone ID Related Accession (Same CDS sequence) XM_024992054.1
    Accession Version XM_024992054.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1422bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product LIM domain and actin-binding protein 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGAATGAGT TGAGAGCCAG TGGTGGTGAA ATCAAAACTC ATAAATTGGA GCAAAAGGAG 
    AATGTGCCCC CAGGTCCTGA GGTCTGCATC TCCTACCCAG ATGGAGAAAA GGTTTCTGCA
    TCTGAGAATA GCCTGGCAGC CCATTCCACC CTTGCTGAAG ATGATTCCCG TAACTCCCAG
    GTCAAGAGTG AGGTCCAGCA GCCTGTGCAT CCCAAGCCAT TAAGTCCAGA TGCCAGAGCC
    TCCAGCCTTT CTGAAAGTTC TCCTCCCAAA GCAGTGAAGA AGTTTCAGGC ACCCGCAAGA
    GAGACCTGCG TGGAATGTCA GAAAACAGTC TACTCGATGG AGCGTCTCTT GGCCAACCAG
    CAGGTGTTTC ACATCAGCTG CTTCCGTTGT TCCTATTGCA ACAGCAAGCT TAGTCTAGGA
    ACTTATGCCT CTTTACATGG GAGAATCTAT TGCAAGCCTC ACTTCAATCA ACTCTTTAAA
    TCTAAAGGTA ACTATGATGA AGGCTTTGGA CACAGACCAC ACAAGGATCT GTGGGCAAGC
    AAAAATGAAA ATGAAGAGAC ATTGGAGAGA CCAGTCCAGC TTCCAAGTGC AGCGGAGACC
    CTTCAGAGCC CAGGGGTAGA AAATGCCCCC ATTGAGAAAG TGGGTGTGCT AACTGCGAGT
    ATGGAAGCCA AGGCCTCCTG CCTACTGGAG AAGGAAGACA AGCCAGCTGA AACCAAGAAG
    CTGAGGATCG CCTGGCCACC CCCCACTGAG CTTGGCAGTT CAGGAAGTGC CTTGGAAGAA
    GGGATCAAAG TATTGAAGCC TAAGTGGCCT CCTGAGGATG ATATTAGCAA GCCTGAAGCT
    CCTGAGGATG TAGACCTGGA TCTGAAGAAG CTAAGACGAT CTTCTTCTCT GAAGGAGAGA
    AGCCGCCCAT TCACCGTAGC AGCTTCATTT CGAACCTCTT CAGTCAAGAG CCCAAAAGCT
    TTAAACCCAC CTATCAGGAA GGGCTGGAGC ATGTCAGAGC AAAGCGAGGA GTATGGAGGA
    GGTGTAGCAG CAGAAAGGAA ACACATGGAA AATGCCAACG CCTCTGAGAA GAATGAGAGT
    GTGGGGAAAA CCACCTGGCC CAACAAGGGA TCTAAAGGAG GGGAGGCTGG GCGGAGAAGC
    AGGGAGAGTC CTAGTTTTGG AATGGGGAGT GAAAATCTTA TAGAGAATGG TGCAAACTTA
    GATGAAAGTG ATAAAAACCT TCTCAAAAAG CAGTCTCCAC TAGAACCCAA GGCAAAAACC
    TGGTCTAGCT ATACAGACAA CCCCTCCACT AAAGAATTCA CTACTCAAAC TCAGAAATCC
    CAGGATGTGG GATTCTGGGA GGAAGAGGTA GTCGAAGAGC TGTCTGTCGA AGAACAGATA
    AAGAGAAATC GGTACTATGA TGAGGATGAA GATGAAGAAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024847822.1
    CDS924..2345
    Translation

    Target ORF information:

    RefSeq Version XM_024992054.1
    Organism Bos taurus(cattle)
    Definition Bos taurus LIM domain and actin binding 1 (LIMA1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024992054.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGAATGAGT TGAGAGCCAG TGGTGGTGAA ATCAAAACTC ATAAATTGGA GCAAAAGGAG 
    AATGTGCCCC CAGGTCCTGA GGTCTGCATC TCCTACCCAG ATGGAGAAAA GGTTTCTGCA
    TCTGAGAATA GCCTGGCAGC CCATTCCACC CTTGCTGAAG ATGATTCCCG TAACTCCCAG
    GTCAAGAGTG AGGTCCAGCA GCCTGTGCAT CCCAAGCCAT TAAGTCCAGA TGCCAGAGCC
    TCCAGCCTTT CTGAAAGTTC TCCTCCCAAA GCAGTGAAGA AGTTTCAGGC ACCCGCAAGA
    GAGACCTGCG TGGAATGTCA GAAAACAGTC TACTCGATGG AGCGTCTCTT GGCCAACCAG
    CAGGTGTTTC ACATCAGCTG CTTCCGTTGT TCCTATTGCA ACAGCAAGCT TAGTCTAGGA
    ACTTATGCCT CTTTACATGG GAGAATCTAT TGCAAGCCTC ACTTCAATCA ACTCTTTAAA
    TCTAAAGGTA ACTATGATGA AGGCTTTGGA CACAGACCAC ACAAGGATCT GTGGGCAAGC
    AAAAATGAAA ATGAAGAGAC ATTGGAGAGA CCAGTCCAGC TTCCAAGTGC AGCGGAGACC
    CTTCAGAGCC CAGGGGTAGA AAATGCCCCC ATTGAGAAAG TGGGTGTGCT AACTGCGAGT
    ATGGAAGCCA AGGCCTCCTG CCTACTGGAG AAGGAAGACA AGCCAGCTGA AACCAAGAAG
    CTGAGGATCG CCTGGCCACC CCCCACTGAG CTTGGCAGTT CAGGAAGTGC CTTGGAAGAA
    GGGATCAAAG TATTGAAGCC TAAGTGGCCT CCTGAGGATG ATATTAGCAA GCCTGAAGCT
    CCTGAGGATG TAGACCTGGA TCTGAAGAAG CTAAGACGAT CTTCTTCTCT GAAGGAGAGA
    AGCCGCCCAT TCACCGTAGC AGCTTCATTT CGAACCTCTT CAGTCAAGAG CCCAAAAGCT
    TTAAACCCAC CTATCAGGAA GGGCTGGAGC ATGTCAGAGC AAAGCGAGGA GTATGGAGGA
    GGTGTAGCAG CAGAAAGGAA ACACATGGAA AATGCCAACG CCTCTGAGAA GAATGAGAGT
    GTGGGGAAAA CCACCTGGCC CAACAAGGGA TCTAAAGGAG GGGAGGCTGG GCGGAGAAGC
    AGGGAGAGTC CTAGTTTTGG AATGGGGAGT GAAAATCTTA TAGAGAATGG TGCAAACTTA
    GATGAAAGTG ATAAAAACCT TCTCAAAAAG CAGTCTCCAC TAGAACCCAA GGCAAAAACC
    TGGTCTAGCT ATACAGACAA CCCCTCCACT AAAGAATTCA CTACTCAAAC TCAGAAATCC
    CAGGATGTGG GATTCTGGGA GGAAGAGGTA GTCGAAGAGC TGTCTGTCGA AGAACAGATA
    AAGAGAAATC GGTACTATGA TGAGGATGAA GATGAAGAAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa37169
    Clone ID Related Accession (Same CDS sequence) NM_001192754.1
    Accession Version NM_001192754.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2289bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-29
    Organism Bos taurus(cattle)
    Product LIM domain and actin-binding protein 1
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000005.1. On Jul 17, 2010 this sequence version replaced XM_612416.5. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN03145413, SAMN03145414 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGAACCAA CTCCATTTAA CAGACGGCAA TGGGCTTCTT TATCGTTGAG GGTTACAGCC 
    AAAGAACTTT CCCTTGTCAA CAAGAACAAC TCATCGGCCA TTGTAGAAAT ATTCTCCAAG
    TACCAGAAAG CAGCGGAAGA AGCAAACATG GAAAAGAAAA GAAGTAACGC TGAAAATCTC
    CCCCAGCACT TTAGAAGGGG GAACCTGACT GCGTTAAGGA AGAAGTGGGA GAACCCCGTG
    CTGGGAGCCG ACTCTCTCCC AGACTCAGTG CGAAACAGCA GCGCAGAGGT CAGGCACAGA
    GGCGACCCTC CTCCCGCTGA AGTGGTGGGC AGCTCTGCCT CTGGGGTCCA GGCTGACCGA
    GAGGAGCTAG TCCAACCCAG ACCTAAGATC AGATCGCCTC CTGAAGCTCC TACTAAGTAC
    CCGCACCCCC GCATCAAGGA CAGTGAGCAT CTTAAAGACC ACTCAACAGA AAATAAAAAG
    ATGGAAAACT GTCTGGGAGA ATCCAGACAT GAAGTAGCAA AACCCGAAAT GAGTGAAAAC
    GCAGAAACTG CAAACAAAAT AGAGAAATAC AATGTTCCGC TGAACAGGCT GAAGATGATG
    TTTGAGAAAG GTGAACCAAC TCAAACCAAG ATTCCTCGGG GCCAAAGCCG AAGTGCAGGT
    GGAAGGAAGA TGTCTGAAAA TAGCTATTCT CTGGATGACT TGGAAATAGG CCCAGGTCAG
    CTGTCATCTT CAGCACTCAA CTCAGAGAAA AGAGAGAGTA GGCGAAATCT AGAATTTCCA
    CGCCTGTCAG AAACCTCCAT AAAGGATCGA ATGGCCAAGT ACCAGGCAGC TGTGTCCAAG
    CAAAGCAGTT CTTCGAGCTA TACAAATGAG TTGAGAGCCA GTGGTGGTGA AATCAAAACT
    CATAAATTGG AGCAAAAGGA GAATGTGCCC CCAGGTCCTG AGGTCTGCAT CTCCTACCCA
    GATGGAGAAA AGGTTTCTGC ATCTGAGAAT AGCCTGGCAG CCCATTCCAC CCTTGCTGAA
    GATGATTCCC GTAAGTGTAA CTCCCAGGTC AAGAGTGAGG TCCAGCAGCC TGTGCATCCC
    AAGCCATTAA GTCCAGATGC CAGAGCCTCC AGCCTTTCTG AAAGTTCTCC TCCCAAAGCA
    GTGAAGAAGT TTCAGGCACC CGCAAGAGAG ACCTGCGTGG AATGTCAGAA AACAGTCTAC
    TCGATGGAGC GTCTCTTGGC CAACCAGCAG GTGTTTCACA TCAGCTGCTT CCGTTGTTCC
    TATTGCAACA GCAAGCTTAG TCTAGGAACT TATGCCTCTT TACATGGGAG AATCTATTGC
    AAGCCTCACT TCAATCAACT CTTTAAATCT AAAGGTAACT ATGATGAAGG CTTTGGACAC
    AGACCACACA AGGATCTGTG GGCAAGCAAA AATGAAAATG AAGAGACATT GGAGAGACCA
    GTCCAGCTTC CAAGTGCAGC GGAGACCCTT CAGAGCCCAG GGGTAGAAAA TGCCCCCATT
    GAGAAAGTGG GTGTGCTAAC TGCGAGTATG GAAGCCAAGG CCTCCTGCCT ACTGGAGAAG
    GAAGACAAGC CAGCTGAAAC CAAGAAGCTG AGGATCGCCT GGCCACCCCC CACTGAGCTT
    GGCAGTTCAG GAAGTGCCTT GGAAGAAGGG ATCAAAGTAT TGAAGCCTAA GTGGCCTCCT
    GAGGATGATA TTAGCAAGCC TGAAGCTCCT GAGGATGTAG ACCTGGATCT GAAGAAGCTA
    AGACGATCTT CTTCTCTGAA GGAGAGAAGC CGCCCATTCA CCGTAGCAGC TTCATTTCGA
    ACCTCTTCAG TCAAGAGCCC AAAAGCTTTA AACCCACCTA TCAGGAAGGG CTGGAGCATG
    TCAGAGCAAA GCGAGGAGTA TGGAGGAGGT GTAGCAGCAG AAAGGAAACA CATGGAAAAT
    GCCAACGCCT CTGAGAAGAA TGAGAGTGTG GGGAAAACCA CCTGGCCCAA CAAGGGATCT
    AAAGGAGGGG AGGCTGGGCG GAGAAGCAGG GAGAGTCCTA GTTTTGGAAT GGGGAGTGAA
    AATCTTATAG AGAATGGTGC AAACTTAGAT GAAAGTGATA AAAACCTTCT CAAAAAGCAG
    TCTCCACTAG AACCCAAGGC AAAAACCTGG TCTAGCTATA CAGACAACCC CTCCACTAAA
    GAATTCACTA CTCAAACTCA GAAATCCCAG GATGTGGGAT TCTGGGAGGA AGAGGTAGTC
    GAAGAGCTGT CTGTCGAAGA ACAGATAAAG AGAAATCGGT ACTATGATGA GGATGAAGAT
    GAAGAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001179683.1
    CDS89..2377
    Translation

    Target ORF information:

    RefSeq Version NM_001192754.1
    Organism Bos taurus(cattle)
    Definition Bos taurus LIM domain and actin binding 1 (LIMA1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001192754.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGAACCAA CTCCATTTAA CAGACGGCAA TGGGCTTCTT TATCGTTGAG GGTTACAGCC 
    AAAGAACTTT CCCTTGTCAA CAAGAACAAC TCATCGGCCA TTGTAGAAAT ATTCTCCAAG
    TACCAGAAAG CAGCGGAAGA AGCAAACATG GAAAAGAAAA GAAGTAACGC TGAAAATCTC
    CCCCAGCACT TTAGAAGGGG GAACCTGACT GCGTTAAGGA AGAAGTGGGA GAACCCCGTG
    CTGGGAGCCG ACTCTCTCCC AGACTCAGTG CGAAACAGCA GCGCAGAGGT CAGGCACAGA
    GGCGACCCTC CTCCCGCTGA AGTGGTGGGC AGCTCTGCCT CTGGGGTCCA GGCTGACCGA
    GAGGAGCTAG TCCAACCCAG ACCTAAGATC AGATCGCCTC CTGAAGCTCC TACTAAGTAC
    CCGCACCCCC GCATCAAGGA CAGTGAGCAT CTTAAAGACC ACTCAACAGA AAATAAAAAG
    ATGGAAAACT GTCTGGGAGA ATCCAGACAT GAAGTAGCAA AACCCGAAAT GAGTGAAAAC
    GCAGAAACTG CAAACAAAAT AGAGAAATAC AATGTTCCGC TGAACAGGCT GAAGATGATG
    TTTGAGAAAG GTGAACCAAC TCAAACCAAG ATTCCTCGGG GCCAAAGCCG AAGTGCAGGT
    GGAAGGAAGA TGTCTGAAAA TAGCTATTCT CTGGATGACT TGGAAATAGG CCCAGGTCAG
    CTGTCATCTT CAGCACTCAA CTCAGAGAAA AGAGAGAGTA GGCGAAATCT AGAATTTCCA
    CGCCTGTCAG AAACCTCCAT AAAGGATCGA ATGGCCAAGT ACCAGGCAGC TGTGTCCAAG
    CAAAGCAGTT CTTCGAGCTA TACAAATGAG TTGAGAGCCA GTGGTGGTGA AATCAAAACT
    CATAAATTGG AGCAAAAGGA GAATGTGCCC CCAGGTCCTG AGGTCTGCAT CTCCTACCCA
    GATGGAGAAA AGGTTTCTGC ATCTGAGAAT AGCCTGGCAG CCCATTCCAC CCTTGCTGAA
    GATGATTCCC GTAAGTGTAA CTCCCAGGTC AAGAGTGAGG TCCAGCAGCC TGTGCATCCC
    AAGCCATTAA GTCCAGATGC CAGAGCCTCC AGCCTTTCTG AAAGTTCTCC TCCCAAAGCA
    GTGAAGAAGT TTCAGGCACC CGCAAGAGAG ACCTGCGTGG AATGTCAGAA AACAGTCTAC
    TCGATGGAGC GTCTCTTGGC CAACCAGCAG GTGTTTCACA TCAGCTGCTT CCGTTGTTCC
    TATTGCAACA GCAAGCTTAG TCTAGGAACT TATGCCTCTT TACATGGGAG AATCTATTGC
    AAGCCTCACT TCAATCAACT CTTTAAATCT AAAGGTAACT ATGATGAAGG CTTTGGACAC
    AGACCACACA AGGATCTGTG GGCAAGCAAA AATGAAAATG AAGAGACATT GGAGAGACCA
    GTCCAGCTTC CAAGTGCAGC GGAGACCCTT CAGAGCCCAG GGGTAGAAAA TGCCCCCATT
    GAGAAAGTGG GTGTGCTAAC TGCGAGTATG GAAGCCAAGG CCTCCTGCCT ACTGGAGAAG
    GAAGACAAGC CAGCTGAAAC CAAGAAGCTG AGGATCGCCT GGCCACCCCC CACTGAGCTT
    GGCAGTTCAG GAAGTGCCTT GGAAGAAGGG ATCAAAGTAT TGAAGCCTAA GTGGCCTCCT
    GAGGATGATA TTAGCAAGCC TGAAGCTCCT GAGGATGTAG ACCTGGATCT GAAGAAGCTA
    AGACGATCTT CTTCTCTGAA GGAGAGAAGC CGCCCATTCA CCGTAGCAGC TTCATTTCGA
    ACCTCTTCAG TCAAGAGCCC AAAAGCTTTA AACCCACCTA TCAGGAAGGG CTGGAGCATG
    TCAGAGCAAA GCGAGGAGTA TGGAGGAGGT GTAGCAGCAG AAAGGAAACA CATGGAAAAT
    GCCAACGCCT CTGAGAAGAA TGAGAGTGTG GGGAAAACCA CCTGGCCCAA CAAGGGATCT
    AAAGGAGGGG AGGCTGGGCG GAGAAGCAGG GAGAGTCCTA GTTTTGGAAT GGGGAGTGAA
    AATCTTATAG AGAATGGTGC AAACTTAGAT GAAAGTGATA AAAACCTTCT CAAAAAGCAG
    TCTCCACTAG AACCCAAGGC AAAAACCTGG TCTAGCTATA CAGACAACCC CTCCACTAAA
    GAATTCACTA CTCAAACTCA GAAATCCCAG GATGTGGGAT TCTGGGAGGA AGAGGTAGTC
    GAAGAGCTGT CTGTCGAAGA ACAGATAAAG AGAAATCGGT ACTATGATGA GGATGAAGAT
    GAAGAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa19234
    Clone ID Related Accession (Same CDS sequence) XM_005206324.4 , XM_015470991.2 , XM_015470991.1 , XM_005206324.3
    Accession Version XM_005206324.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2286bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product LIM domain and actin-binding protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005206324.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGAACCAA CTCCATTTAA CAGACGGCAA TGGGCTTCTT TATCGTTGAG GGTTACAGCC 
    AAAGAACTTT CCCTTGTCAA CAAGAACAAC TCATCGGCCA TTGTAGAAAT ATTCTCCAAG
    TACCAGAAAG CAGCGGAAGA AGCAAACATG GAAAAGAAAA GAAGTAACGC TGAAAATCTC
    CCCCAGCACT TTAGAAGGGG GAACCTGACT GCGTTAAGGA AGAAGTGGGA GAACCCCGTG
    CTGGGAGCCG ACTCTCTCCC AGACTCAGTG CGAAACAGCA GCGCAGAGGT CAGGCACAGA
    GGCGACCCTC CTCCCGCTGA AGTGGTGGGC AGCTCTGCCT CTGGGGTCCA GGCTGACCGA
    GAGGAGCTAG TCCAACCCAG ACCTAAGATC AGATCGCCTC CTGAAGCTCC TACTAAGTAC
    CCGCACCCCC GCATCAAGGA CAGTGAGCAT CTTAAAGACC ACTCAACAGA AAATAAAAAG
    ATGGAAAACT GTCTGGGAGA ATCCAGACAT GAAGTAGCAA AACCCGAAAT GAGTGAAAAC
    GCAGAAACTG CAAACAAAAT AGAGAAATAC AATGTTCCGC TGAACAGGCT GAAGATGATG
    TTTGAGAAAG GTGAACCAAC TCAAACCAAG ATTCCTCGGG GCCAAAGCCG AAGTGCAGGT
    GGAAGGAAGA TGTCTGAAAA TAGCTATTCT CTGGATGACT TGGAAATAGG CCCAGGTCAG
    CTGTCATCTT CAGCACTCAA CTCAGAGAAA AGAGAGAGTA GGCGAAATCT AGAATTTCCA
    CGCCTGTCAG AAACCTCCAT AAAGGATCGA ATGGCCAAGT ACCAGGCAGC TGTGTCCAAG
    CAAAGCAGTT CTTCGAGCTA TACAAATGAG TTGAGAGCCA GTGGTGGTGA AATCAAAACT
    CATAAATTGG AGCAAAAGGA GAATGTGCCC CCAGGTCCTG AGGTCTGCAT CTCCTACCCA
    GATGGAGAAA AGGTTTCTGC ATCTGAGAAT AGCCTGGCAG CCCATTCCAC CCTTGCTGAA
    GATGATTCCC CAGGTAACTC CCAGGTCAAG AGTGAGGTCC AGCAGCCTGT GCATCCCAAG
    CCATTAAGTC CAGATGCCAG AGCCTCCAGC CTTTCTGAAA GTTCTCCTCC CAAAGCAGTG
    AAGAAGTTTC AGGCACCCGC AAGAGAGACC TGCGTGGAAT GTCAGAAAAC AGTCTACTCG
    ATGGAGCGTC TCTTGGCCAA CCAGCAGGTG TTTCACATCA GCTGCTTCCG TTGTTCCTAT
    TGCAACAGCA AGCTTAGTCT AGGAACTTAT GCCTCTTTAC ATGGGAGAAT CTATTGCAAG
    CCTCACTTCA ATCAACTCTT TAAATCTAAA GGTAACTATG ATGAAGGCTT TGGACACAGA
    CCACACAAGG ATCTGTGGGC AAGCAAAAAT GAAAATGAAG AGACATTGGA GAGACCAGTC
    CAGCTTCCAA GTGCAGCGGA GACCCTTCAG AGCCCAGGGG TAGAAAATGC CCCCATTGAG
    AAAGTGGGTG TGCTAACTGC GAGTATGGAA GCCAAGGCCT CCTGCCTACT GGAGAAGGAA
    GACAAGCCAG CTGAAACCAA GAAGCTGAGG ATCGCCTGGC CACCCCCCAC TGAGCTTGGC
    AGTTCAGGAA GTGCCTTGGA AGAAGGGATC AAAGTATTGA AGCCTAAGTG GCCTCCTGAG
    GATGATATTA GCAAGCCTGA AGCTCCTGAG GATGTAGACC TGGATCTGAA GAAGCTAAGA
    CGATCTTCTT CTCTGAAGGA GAGAAGCCGC CCATTCACCG TAGCAGCTTC ATTTCGAACC
    TCTTCAGTCA AGAGCCCAAA AGCTTTAAAC CCACCTATCA GGAAGGGCTG GAGCATGTCA
    GAGCAAAGCG AGGAGTATGG AGGAGGTGTA GCAGCAGAAA GGAAACACAT GGAAAATGCC
    AACGCCTCTG AGAAGAATGA GAGTGTGGGG AAAACCACCT GGCCCAACAA GGGATCTAAA
    GGAGGGGAGG CTGGGCGGAG AAGCAGGGAG AGTCCTAGTT TTGGAATGGG GAGTGAAAAT
    CTTATAGAGA ATGGTGCAAA CTTAGATGAA AGTGATAAAA ACCTTCTCAA AAAGCAGTCT
    CCACTAGAAC CCAAGGCAAA AACCTGGTCT AGCTATACAG ACAACCCCTC CACTAAAGAA
    TTCACTACTC AAACTCAGAA ATCCCAGGAT GTGGGATTCT GGGAGGAAGA GGTAGTCGAA
    GAGCTGTCTG TCGAAGAACA GATAAAGAGA AATCGGTACT ATGATGAGGA TGAAGATGAA
    GAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005206381.1
    CDS159..2444
    Translation

    Target ORF information:

    RefSeq Version XM_005206324.4
    Organism Bos taurus(cattle)
    Definition Bos taurus LIM domain and actin binding 1 (LIMA1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005206324.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGAACCAA CTCCATTTAA CAGACGGCAA TGGGCTTCTT TATCGTTGAG GGTTACAGCC 
    AAAGAACTTT CCCTTGTCAA CAAGAACAAC TCATCGGCCA TTGTAGAAAT ATTCTCCAAG
    TACCAGAAAG CAGCGGAAGA AGCAAACATG GAAAAGAAAA GAAGTAACGC TGAAAATCTC
    CCCCAGCACT TTAGAAGGGG GAACCTGACT GCGTTAAGGA AGAAGTGGGA GAACCCCGTG
    CTGGGAGCCG ACTCTCTCCC AGACTCAGTG CGAAACAGCA GCGCAGAGGT CAGGCACAGA
    GGCGACCCTC CTCCCGCTGA AGTGGTGGGC AGCTCTGCCT CTGGGGTCCA GGCTGACCGA
    GAGGAGCTAG TCCAACCCAG ACCTAAGATC AGATCGCCTC CTGAAGCTCC TACTAAGTAC
    CCGCACCCCC GCATCAAGGA CAGTGAGCAT CTTAAAGACC ACTCAACAGA AAATAAAAAG
    ATGGAAAACT GTCTGGGAGA ATCCAGACAT GAAGTAGCAA AACCCGAAAT GAGTGAAAAC
    GCAGAAACTG CAAACAAAAT AGAGAAATAC AATGTTCCGC TGAACAGGCT GAAGATGATG
    TTTGAGAAAG GTGAACCAAC TCAAACCAAG ATTCCTCGGG GCCAAAGCCG AAGTGCAGGT
    GGAAGGAAGA TGTCTGAAAA TAGCTATTCT CTGGATGACT TGGAAATAGG CCCAGGTCAG
    CTGTCATCTT CAGCACTCAA CTCAGAGAAA AGAGAGAGTA GGCGAAATCT AGAATTTCCA
    CGCCTGTCAG AAACCTCCAT AAAGGATCGA ATGGCCAAGT ACCAGGCAGC TGTGTCCAAG
    CAAAGCAGTT CTTCGAGCTA TACAAATGAG TTGAGAGCCA GTGGTGGTGA AATCAAAACT
    CATAAATTGG AGCAAAAGGA GAATGTGCCC CCAGGTCCTG AGGTCTGCAT CTCCTACCCA
    GATGGAGAAA AGGTTTCTGC ATCTGAGAAT AGCCTGGCAG CCCATTCCAC CCTTGCTGAA
    GATGATTCCC CAGGTAACTC CCAGGTCAAG AGTGAGGTCC AGCAGCCTGT GCATCCCAAG
    CCATTAAGTC CAGATGCCAG AGCCTCCAGC CTTTCTGAAA GTTCTCCTCC CAAAGCAGTG
    AAGAAGTTTC AGGCACCCGC AAGAGAGACC TGCGTGGAAT GTCAGAAAAC AGTCTACTCG
    ATGGAGCGTC TCTTGGCCAA CCAGCAGGTG TTTCACATCA GCTGCTTCCG TTGTTCCTAT
    TGCAACAGCA AGCTTAGTCT AGGAACTTAT GCCTCTTTAC ATGGGAGAAT CTATTGCAAG
    CCTCACTTCA ATCAACTCTT TAAATCTAAA GGTAACTATG ATGAAGGCTT TGGACACAGA
    CCACACAAGG ATCTGTGGGC AAGCAAAAAT GAAAATGAAG AGACATTGGA GAGACCAGTC
    CAGCTTCCAA GTGCAGCGGA GACCCTTCAG AGCCCAGGGG TAGAAAATGC CCCCATTGAG
    AAAGTGGGTG TGCTAACTGC GAGTATGGAA GCCAAGGCCT CCTGCCTACT GGAGAAGGAA
    GACAAGCCAG CTGAAACCAA GAAGCTGAGG ATCGCCTGGC CACCCCCCAC TGAGCTTGGC
    AGTTCAGGAA GTGCCTTGGA AGAAGGGATC AAAGTATTGA AGCCTAAGTG GCCTCCTGAG
    GATGATATTA GCAAGCCTGA AGCTCCTGAG GATGTAGACC TGGATCTGAA GAAGCTAAGA
    CGATCTTCTT CTCTGAAGGA GAGAAGCCGC CCATTCACCG TAGCAGCTTC ATTTCGAACC
    TCTTCAGTCA AGAGCCCAAA AGCTTTAAAC CCACCTATCA GGAAGGGCTG GAGCATGTCA
    GAGCAAAGCG AGGAGTATGG AGGAGGTGTA GCAGCAGAAA GGAAACACAT GGAAAATGCC
    AACGCCTCTG AGAAGAATGA GAGTGTGGGG AAAACCACCT GGCCCAACAA GGGATCTAAA
    GGAGGGGAGG CTGGGCGGAG AAGCAGGGAG AGTCCTAGTT TTGGAATGGG GAGTGAAAAT
    CTTATAGAGA ATGGTGCAAA CTTAGATGAA AGTGATAAAA ACCTTCTCAA AAAGCAGTCT
    CCACTAGAAC CCAAGGCAAA AACCTGGTCT AGCTATACAG ACAACCCCTC CACTAAAGAA
    TTCACTACTC AAACTCAGAA ATCCCAGGAT GTGGGATTCT GGGAGGAAGA GGTAGTCGAA
    GAGCTGTCTG TCGAAGAACA GATAAAGAGA AATCGGTACT ATGATGAGGA TGAAGATGAA
    GAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa19234
    Clone ID Related Accession (Same CDS sequence) XM_005206324.4 , XM_015470991.2 , XM_015470991.1 , XM_005206324.3
    Accession Version XM_015470991.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2286bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product LIM domain and actin-binding protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015470991.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGAACCAA CTCCATTTAA CAGACGGCAA TGGGCTTCTT TATCGTTGAG GGTTACAGCC 
    AAAGAACTTT CCCTTGTCAA CAAGAACAAC TCATCGGCCA TTGTAGAAAT ATTCTCCAAG
    TACCAGAAAG CAGCGGAAGA AGCAAACATG GAAAAGAAAA GAAGTAACGC TGAAAATCTC
    CCCCAGCACT TTAGAAGGGG GAACCTGACT GCGTTAAGGA AGAAGTGGGA GAACCCCGTG
    CTGGGAGCCG ACTCTCTCCC AGACTCAGTG CGAAACAGCA GCGCAGAGGT CAGGCACAGA
    GGCGACCCTC CTCCCGCTGA AGTGGTGGGC AGCTCTGCCT CTGGGGTCCA GGCTGACCGA
    GAGGAGCTAG TCCAACCCAG ACCTAAGATC AGATCGCCTC CTGAAGCTCC TACTAAGTAC
    CCGCACCCCC GCATCAAGGA CAGTGAGCAT CTTAAAGACC ACTCAACAGA AAATAAAAAG
    ATGGAAAACT GTCTGGGAGA ATCCAGACAT GAAGTAGCAA AACCCGAAAT GAGTGAAAAC
    GCAGAAACTG CAAACAAAAT AGAGAAATAC AATGTTCCGC TGAACAGGCT GAAGATGATG
    TTTGAGAAAG GTGAACCAAC TCAAACCAAG ATTCCTCGGG GCCAAAGCCG AAGTGCAGGT
    GGAAGGAAGA TGTCTGAAAA TAGCTATTCT CTGGATGACT TGGAAATAGG CCCAGGTCAG
    CTGTCATCTT CAGCACTCAA CTCAGAGAAA AGAGAGAGTA GGCGAAATCT AGAATTTCCA
    CGCCTGTCAG AAACCTCCAT AAAGGATCGA ATGGCCAAGT ACCAGGCAGC TGTGTCCAAG
    CAAAGCAGTT CTTCGAGCTA TACAAATGAG TTGAGAGCCA GTGGTGGTGA AATCAAAACT
    CATAAATTGG AGCAAAAGGA GAATGTGCCC CCAGGTCCTG AGGTCTGCAT CTCCTACCCA
    GATGGAGAAA AGGTTTCTGC ATCTGAGAAT AGCCTGGCAG CCCATTCCAC CCTTGCTGAA
    GATGATTCCC CAGGTAACTC CCAGGTCAAG AGTGAGGTCC AGCAGCCTGT GCATCCCAAG
    CCATTAAGTC CAGATGCCAG AGCCTCCAGC CTTTCTGAAA GTTCTCCTCC CAAAGCAGTG
    AAGAAGTTTC AGGCACCCGC AAGAGAGACC TGCGTGGAAT GTCAGAAAAC AGTCTACTCG
    ATGGAGCGTC TCTTGGCCAA CCAGCAGGTG TTTCACATCA GCTGCTTCCG TTGTTCCTAT
    TGCAACAGCA AGCTTAGTCT AGGAACTTAT GCCTCTTTAC ATGGGAGAAT CTATTGCAAG
    CCTCACTTCA ATCAACTCTT TAAATCTAAA GGTAACTATG ATGAAGGCTT TGGACACAGA
    CCACACAAGG ATCTGTGGGC AAGCAAAAAT GAAAATGAAG AGACATTGGA GAGACCAGTC
    CAGCTTCCAA GTGCAGCGGA GACCCTTCAG AGCCCAGGGG TAGAAAATGC CCCCATTGAG
    AAAGTGGGTG TGCTAACTGC GAGTATGGAA GCCAAGGCCT CCTGCCTACT GGAGAAGGAA
    GACAAGCCAG CTGAAACCAA GAAGCTGAGG ATCGCCTGGC CACCCCCCAC TGAGCTTGGC
    AGTTCAGGAA GTGCCTTGGA AGAAGGGATC AAAGTATTGA AGCCTAAGTG GCCTCCTGAG
    GATGATATTA GCAAGCCTGA AGCTCCTGAG GATGTAGACC TGGATCTGAA GAAGCTAAGA
    CGATCTTCTT CTCTGAAGGA GAGAAGCCGC CCATTCACCG TAGCAGCTTC ATTTCGAACC
    TCTTCAGTCA AGAGCCCAAA AGCTTTAAAC CCACCTATCA GGAAGGGCTG GAGCATGTCA
    GAGCAAAGCG AGGAGTATGG AGGAGGTGTA GCAGCAGAAA GGAAACACAT GGAAAATGCC
    AACGCCTCTG AGAAGAATGA GAGTGTGGGG AAAACCACCT GGCCCAACAA GGGATCTAAA
    GGAGGGGAGG CTGGGCGGAG AAGCAGGGAG AGTCCTAGTT TTGGAATGGG GAGTGAAAAT
    CTTATAGAGA ATGGTGCAAA CTTAGATGAA AGTGATAAAA ACCTTCTCAA AAAGCAGTCT
    CCACTAGAAC CCAAGGCAAA AACCTGGTCT AGCTATACAG ACAACCCCTC CACTAAAGAA
    TTCACTACTC AAACTCAGAA ATCCCAGGAT GTGGGATTCT GGGAGGAAGA GGTAGTCGAA
    GAGCTGTCTG TCGAAGAACA GATAAAGAGA AATCGGTACT ATGATGAGGA TGAAGATGAA
    GAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015326477.1
    CDS97..2382
    Translation

    Target ORF information:

    RefSeq Version XM_015470991.2
    Organism Bos taurus(cattle)
    Definition Bos taurus LIM domain and actin binding 1 (LIMA1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015470991.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGAACCAA CTCCATTTAA CAGACGGCAA TGGGCTTCTT TATCGTTGAG GGTTACAGCC 
    AAAGAACTTT CCCTTGTCAA CAAGAACAAC TCATCGGCCA TTGTAGAAAT ATTCTCCAAG
    TACCAGAAAG CAGCGGAAGA AGCAAACATG GAAAAGAAAA GAAGTAACGC TGAAAATCTC
    CCCCAGCACT TTAGAAGGGG GAACCTGACT GCGTTAAGGA AGAAGTGGGA GAACCCCGTG
    CTGGGAGCCG ACTCTCTCCC AGACTCAGTG CGAAACAGCA GCGCAGAGGT CAGGCACAGA
    GGCGACCCTC CTCCCGCTGA AGTGGTGGGC AGCTCTGCCT CTGGGGTCCA GGCTGACCGA
    GAGGAGCTAG TCCAACCCAG ACCTAAGATC AGATCGCCTC CTGAAGCTCC TACTAAGTAC
    CCGCACCCCC GCATCAAGGA CAGTGAGCAT CTTAAAGACC ACTCAACAGA AAATAAAAAG
    ATGGAAAACT GTCTGGGAGA ATCCAGACAT GAAGTAGCAA AACCCGAAAT GAGTGAAAAC
    GCAGAAACTG CAAACAAAAT AGAGAAATAC AATGTTCCGC TGAACAGGCT GAAGATGATG
    TTTGAGAAAG GTGAACCAAC TCAAACCAAG ATTCCTCGGG GCCAAAGCCG AAGTGCAGGT
    GGAAGGAAGA TGTCTGAAAA TAGCTATTCT CTGGATGACT TGGAAATAGG CCCAGGTCAG
    CTGTCATCTT CAGCACTCAA CTCAGAGAAA AGAGAGAGTA GGCGAAATCT AGAATTTCCA
    CGCCTGTCAG AAACCTCCAT AAAGGATCGA ATGGCCAAGT ACCAGGCAGC TGTGTCCAAG
    CAAAGCAGTT CTTCGAGCTA TACAAATGAG TTGAGAGCCA GTGGTGGTGA AATCAAAACT
    CATAAATTGG AGCAAAAGGA GAATGTGCCC CCAGGTCCTG AGGTCTGCAT CTCCTACCCA
    GATGGAGAAA AGGTTTCTGC ATCTGAGAAT AGCCTGGCAG CCCATTCCAC CCTTGCTGAA
    GATGATTCCC CAGGTAACTC CCAGGTCAAG AGTGAGGTCC AGCAGCCTGT GCATCCCAAG
    CCATTAAGTC CAGATGCCAG AGCCTCCAGC CTTTCTGAAA GTTCTCCTCC CAAAGCAGTG
    AAGAAGTTTC AGGCACCCGC AAGAGAGACC TGCGTGGAAT GTCAGAAAAC AGTCTACTCG
    ATGGAGCGTC TCTTGGCCAA CCAGCAGGTG TTTCACATCA GCTGCTTCCG TTGTTCCTAT
    TGCAACAGCA AGCTTAGTCT AGGAACTTAT GCCTCTTTAC ATGGGAGAAT CTATTGCAAG
    CCTCACTTCA ATCAACTCTT TAAATCTAAA GGTAACTATG ATGAAGGCTT TGGACACAGA
    CCACACAAGG ATCTGTGGGC AAGCAAAAAT GAAAATGAAG AGACATTGGA GAGACCAGTC
    CAGCTTCCAA GTGCAGCGGA GACCCTTCAG AGCCCAGGGG TAGAAAATGC CCCCATTGAG
    AAAGTGGGTG TGCTAACTGC GAGTATGGAA GCCAAGGCCT CCTGCCTACT GGAGAAGGAA
    GACAAGCCAG CTGAAACCAA GAAGCTGAGG ATCGCCTGGC CACCCCCCAC TGAGCTTGGC
    AGTTCAGGAA GTGCCTTGGA AGAAGGGATC AAAGTATTGA AGCCTAAGTG GCCTCCTGAG
    GATGATATTA GCAAGCCTGA AGCTCCTGAG GATGTAGACC TGGATCTGAA GAAGCTAAGA
    CGATCTTCTT CTCTGAAGGA GAGAAGCCGC CCATTCACCG TAGCAGCTTC ATTTCGAACC
    TCTTCAGTCA AGAGCCCAAA AGCTTTAAAC CCACCTATCA GGAAGGGCTG GAGCATGTCA
    GAGCAAAGCG AGGAGTATGG AGGAGGTGTA GCAGCAGAAA GGAAACACAT GGAAAATGCC
    AACGCCTCTG AGAAGAATGA GAGTGTGGGG AAAACCACCT GGCCCAACAA GGGATCTAAA
    GGAGGGGAGG CTGGGCGGAG AAGCAGGGAG AGTCCTAGTT TTGGAATGGG GAGTGAAAAT
    CTTATAGAGA ATGGTGCAAA CTTAGATGAA AGTGATAAAA ACCTTCTCAA AAAGCAGTCT
    CCACTAGAAC CCAAGGCAAA AACCTGGTCT AGCTATACAG ACAACCCCTC CACTAAAGAA
    TTCACTACTC AAACTCAGAA ATCCCAGGAT GTGGGATTCT GGGAGGAAGA GGTAGTCGAA
    GAGCTGTCTG TCGAAGAACA GATAAAGAGA AATCGGTACT ATGATGAGGA TGAAGATGAA
    GAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa19234
    Clone ID Related Accession (Same CDS sequence) XM_005206324.4 , XM_015470991.2 , XM_015470991.1 , XM_005206324.3
    Accession Version XM_015470991.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2286bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product LIM domain and actin-binding protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103920.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGAACCAA CTCCATTTAA CAGACGGCAA TGGGCTTCTT TATCGTTGAG GGTTACAGCC 
    AAAGAACTTT CCCTTGTCAA CAAGAACAAC TCATCGGCCA TTGTAGAAAT ATTCTCCAAG
    TACCAGAAAG CAGCGGAAGA AGCAAACATG GAAAAGAAAA GAAGTAACGC TGAAAATCTC
    CCCCAGCACT TTAGAAGGGG GAACCTGACT GCGTTAAGGA AGAAGTGGGA GAACCCCGTG
    CTGGGAGCCG ACTCTCTCCC AGACTCAGTG CGAAACAGCA GCGCAGAGGT CAGGCACAGA
    GGCGACCCTC CTCCCGCTGA AGTGGTGGGC AGCTCTGCCT CTGGGGTCCA GGCTGACCGA
    GAGGAGCTAG TCCAACCCAG ACCTAAGATC AGATCGCCTC CTGAAGCTCC TACTAAGTAC
    CCGCACCCCC GCATCAAGGA CAGTGAGCAT CTTAAAGACC ACTCAACAGA AAATAAAAAG
    ATGGAAAACT GTCTGGGAGA ATCCAGACAT GAAGTAGCAA AACCCGAAAT GAGTGAAAAC
    GCAGAAACTG CAAACAAAAT AGAGAAATAC AATGTTCCGC TGAACAGGCT GAAGATGATG
    TTTGAGAAAG GTGAACCAAC TCAAACCAAG ATTCCTCGGG GCCAAAGCCG AAGTGCAGGT
    GGAAGGAAGA TGTCTGAAAA TAGCTATTCT CTGGATGACT TGGAAATAGG CCCAGGTCAG
    CTGTCATCTT CAGCACTCAA CTCAGAGAAA AGAGAGAGTA GGCGAAATCT AGAATTTCCA
    CGCCTGTCAG AAACCTCCAT AAAGGATCGA ATGGCCAAGT ACCAGGCAGC TGTGTCCAAG
    CAAAGCAGTT CTTCGAGCTA TACAAATGAG TTGAGAGCCA GTGGTGGTGA AATCAAAACT
    CATAAATTGG AGCAAAAGGA GAATGTGCCC CCAGGTCCTG AGGTCTGCAT CTCCTACCCA
    GATGGAGAAA AGGTTTCTGC ATCTGAGAAT AGCCTGGCAG CCCATTCCAC CCTTGCTGAA
    GATGATTCCC CAGGTAACTC CCAGGTCAAG AGTGAGGTCC AGCAGCCTGT GCATCCCAAG
    CCATTAAGTC CAGATGCCAG AGCCTCCAGC CTTTCTGAAA GTTCTCCTCC CAAAGCAGTG
    AAGAAGTTTC AGGCACCCGC AAGAGAGACC TGCGTGGAAT GTCAGAAAAC AGTCTACTCG
    ATGGAGCGTC TCTTGGCCAA CCAGCAGGTG TTTCACATCA GCTGCTTCCG TTGTTCCTAT
    TGCAACAGCA AGCTTAGTCT AGGAACTTAT GCCTCTTTAC ATGGGAGAAT CTATTGCAAG
    CCTCACTTCA ATCAACTCTT TAAATCTAAA GGTAACTATG ATGAAGGCTT TGGACACAGA
    CCACACAAGG ATCTGTGGGC AAGCAAAAAT GAAAATGAAG AGACATTGGA GAGACCAGTC
    CAGCTTCCAA GTGCAGCGGA GACCCTTCAG AGCCCAGGGG TAGAAAATGC CCCCATTGAG
    AAAGTGGGTG TGCTAACTGC GAGTATGGAA GCCAAGGCCT CCTGCCTACT GGAGAAGGAA
    GACAAGCCAG CTGAAACCAA GAAGCTGAGG ATCGCCTGGC CACCCCCCAC TGAGCTTGGC
    AGTTCAGGAA GTGCCTTGGA AGAAGGGATC AAAGTATTGA AGCCTAAGTG GCCTCCTGAG
    GATGATATTA GCAAGCCTGA AGCTCCTGAG GATGTAGACC TGGATCTGAA GAAGCTAAGA
    CGATCTTCTT CTCTGAAGGA GAGAAGCCGC CCATTCACCG TAGCAGCTTC ATTTCGAACC
    TCTTCAGTCA AGAGCCCAAA AGCTTTAAAC CCACCTATCA GGAAGGGCTG GAGCATGTCA
    GAGCAAAGCG AGGAGTATGG AGGAGGTGTA GCAGCAGAAA GGAAACACAT GGAAAATGCC
    AACGCCTCTG AGAAGAATGA GAGTGTGGGG AAAACCACCT GGCCCAACAA GGGATCTAAA
    GGAGGGGAGG CTGGGCGGAG AAGCAGGGAG AGTCCTAGTT TTGGAATGGG GAGTGAAAAT
    CTTATAGAGA ATGGTGCAAA CTTAGATGAA AGTGATAAAA ACCTTCTCAA AAAGCAGTCT
    CCACTAGAAC CCAAGGCAAA AACCTGGTCT AGCTATACAG ACAACCCCTC CACTAAAGAA
    TTCACTACTC AAACTCAGAA ATCCCAGGAT GTGGGATTCT GGGAGGAAGA GGTAGTCGAA
    GAGCTGTCTG TCGAAGAACA GATAAAGAGA AATCGGTACT ATGATGAGGA TGAAGATGAA
    GAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015326477.1
    CDS112..2397
    Translation

    Target ORF information:

    RefSeq Version XM_015470991.1
    Organism Bos taurus(cattle)
    Definition Bos taurus LIM domain and actin binding 1 (LIMA1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015470991.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGAACCAA CTCCATTTAA CAGACGGCAA TGGGCTTCTT TATCGTTGAG GGTTACAGCC 
    AAAGAACTTT CCCTTGTCAA CAAGAACAAC TCATCGGCCA TTGTAGAAAT ATTCTCCAAG
    TACCAGAAAG CAGCGGAAGA AGCAAACATG GAAAAGAAAA GAAGTAACGC TGAAAATCTC
    CCCCAGCACT TTAGAAGGGG GAACCTGACT GCGTTAAGGA AGAAGTGGGA GAACCCCGTG
    CTGGGAGCCG ACTCTCTCCC AGACTCAGTG CGAAACAGCA GCGCAGAGGT CAGGCACAGA
    GGCGACCCTC CTCCCGCTGA AGTGGTGGGC AGCTCTGCCT CTGGGGTCCA GGCTGACCGA
    GAGGAGCTAG TCCAACCCAG ACCTAAGATC AGATCGCCTC CTGAAGCTCC TACTAAGTAC
    CCGCACCCCC GCATCAAGGA CAGTGAGCAT CTTAAAGACC ACTCAACAGA AAATAAAAAG
    ATGGAAAACT GTCTGGGAGA ATCCAGACAT GAAGTAGCAA AACCCGAAAT GAGTGAAAAC
    GCAGAAACTG CAAACAAAAT AGAGAAATAC AATGTTCCGC TGAACAGGCT GAAGATGATG
    TTTGAGAAAG GTGAACCAAC TCAAACCAAG ATTCCTCGGG GCCAAAGCCG AAGTGCAGGT
    GGAAGGAAGA TGTCTGAAAA TAGCTATTCT CTGGATGACT TGGAAATAGG CCCAGGTCAG
    CTGTCATCTT CAGCACTCAA CTCAGAGAAA AGAGAGAGTA GGCGAAATCT AGAATTTCCA
    CGCCTGTCAG AAACCTCCAT AAAGGATCGA ATGGCCAAGT ACCAGGCAGC TGTGTCCAAG
    CAAAGCAGTT CTTCGAGCTA TACAAATGAG TTGAGAGCCA GTGGTGGTGA AATCAAAACT
    CATAAATTGG AGCAAAAGGA GAATGTGCCC CCAGGTCCTG AGGTCTGCAT CTCCTACCCA
    GATGGAGAAA AGGTTTCTGC ATCTGAGAAT AGCCTGGCAG CCCATTCCAC CCTTGCTGAA
    GATGATTCCC CAGGTAACTC CCAGGTCAAG AGTGAGGTCC AGCAGCCTGT GCATCCCAAG
    CCATTAAGTC CAGATGCCAG AGCCTCCAGC CTTTCTGAAA GTTCTCCTCC CAAAGCAGTG
    AAGAAGTTTC AGGCACCCGC AAGAGAGACC TGCGTGGAAT GTCAGAAAAC AGTCTACTCG
    ATGGAGCGTC TCTTGGCCAA CCAGCAGGTG TTTCACATCA GCTGCTTCCG TTGTTCCTAT
    TGCAACAGCA AGCTTAGTCT AGGAACTTAT GCCTCTTTAC ATGGGAGAAT CTATTGCAAG
    CCTCACTTCA ATCAACTCTT TAAATCTAAA GGTAACTATG ATGAAGGCTT TGGACACAGA
    CCACACAAGG ATCTGTGGGC AAGCAAAAAT GAAAATGAAG AGACATTGGA GAGACCAGTC
    CAGCTTCCAA GTGCAGCGGA GACCCTTCAG AGCCCAGGGG TAGAAAATGC CCCCATTGAG
    AAAGTGGGTG TGCTAACTGC GAGTATGGAA GCCAAGGCCT CCTGCCTACT GGAGAAGGAA
    GACAAGCCAG CTGAAACCAA GAAGCTGAGG ATCGCCTGGC CACCCCCCAC TGAGCTTGGC
    AGTTCAGGAA GTGCCTTGGA AGAAGGGATC AAAGTATTGA AGCCTAAGTG GCCTCCTGAG
    GATGATATTA GCAAGCCTGA AGCTCCTGAG GATGTAGACC TGGATCTGAA GAAGCTAAGA
    CGATCTTCTT CTCTGAAGGA GAGAAGCCGC CCATTCACCG TAGCAGCTTC ATTTCGAACC
    TCTTCAGTCA AGAGCCCAAA AGCTTTAAAC CCACCTATCA GGAAGGGCTG GAGCATGTCA
    GAGCAAAGCG AGGAGTATGG AGGAGGTGTA GCAGCAGAAA GGAAACACAT GGAAAATGCC
    AACGCCTCTG AGAAGAATGA GAGTGTGGGG AAAACCACCT GGCCCAACAA GGGATCTAAA
    GGAGGGGAGG CTGGGCGGAG AAGCAGGGAG AGTCCTAGTT TTGGAATGGG GAGTGAAAAT
    CTTATAGAGA ATGGTGCAAA CTTAGATGAA AGTGATAAAA ACCTTCTCAA AAAGCAGTCT
    CCACTAGAAC CCAAGGCAAA AACCTGGTCT AGCTATACAG ACAACCCCTC CACTAAAGAA
    TTCACTACTC AAACTCAGAA ATCCCAGGAT GTGGGATTCT GGGAGGAAGA GGTAGTCGAA
    GAGCTGTCTG TCGAAGAACA GATAAAGAGA AATCGGTACT ATGATGAGGA TGAAGATGAA
    GAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa19234
    Clone ID Related Accession (Same CDS sequence) XM_005206324.4 , XM_015470991.2 , XM_015470991.1 , XM_005206324.3
    Accession Version XM_005206324.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2286bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product LIM domain and actin-binding protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103920.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005206324.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGAACCAA CTCCATTTAA CAGACGGCAA TGGGCTTCTT TATCGTTGAG GGTTACAGCC 
    AAAGAACTTT CCCTTGTCAA CAAGAACAAC TCATCGGCCA TTGTAGAAAT ATTCTCCAAG
    TACCAGAAAG CAGCGGAAGA AGCAAACATG GAAAAGAAAA GAAGTAACGC TGAAAATCTC
    CCCCAGCACT TTAGAAGGGG GAACCTGACT GCGTTAAGGA AGAAGTGGGA GAACCCCGTG
    CTGGGAGCCG ACTCTCTCCC AGACTCAGTG CGAAACAGCA GCGCAGAGGT CAGGCACAGA
    GGCGACCCTC CTCCCGCTGA AGTGGTGGGC AGCTCTGCCT CTGGGGTCCA GGCTGACCGA
    GAGGAGCTAG TCCAACCCAG ACCTAAGATC AGATCGCCTC CTGAAGCTCC TACTAAGTAC
    CCGCACCCCC GCATCAAGGA CAGTGAGCAT CTTAAAGACC ACTCAACAGA AAATAAAAAG
    ATGGAAAACT GTCTGGGAGA ATCCAGACAT GAAGTAGCAA AACCCGAAAT GAGTGAAAAC
    GCAGAAACTG CAAACAAAAT AGAGAAATAC AATGTTCCGC TGAACAGGCT GAAGATGATG
    TTTGAGAAAG GTGAACCAAC TCAAACCAAG ATTCCTCGGG GCCAAAGCCG AAGTGCAGGT
    GGAAGGAAGA TGTCTGAAAA TAGCTATTCT CTGGATGACT TGGAAATAGG CCCAGGTCAG
    CTGTCATCTT CAGCACTCAA CTCAGAGAAA AGAGAGAGTA GGCGAAATCT AGAATTTCCA
    CGCCTGTCAG AAACCTCCAT AAAGGATCGA ATGGCCAAGT ACCAGGCAGC TGTGTCCAAG
    CAAAGCAGTT CTTCGAGCTA TACAAATGAG TTGAGAGCCA GTGGTGGTGA AATCAAAACT
    CATAAATTGG AGCAAAAGGA GAATGTGCCC CCAGGTCCTG AGGTCTGCAT CTCCTACCCA
    GATGGAGAAA AGGTTTCTGC ATCTGAGAAT AGCCTGGCAG CCCATTCCAC CCTTGCTGAA
    GATGATTCCC CAGGTAACTC CCAGGTCAAG AGTGAGGTCC AGCAGCCTGT GCATCCCAAG
    CCATTAAGTC CAGATGCCAG AGCCTCCAGC CTTTCTGAAA GTTCTCCTCC CAAAGCAGTG
    AAGAAGTTTC AGGCACCCGC AAGAGAGACC TGCGTGGAAT GTCAGAAAAC AGTCTACTCG
    ATGGAGCGTC TCTTGGCCAA CCAGCAGGTG TTTCACATCA GCTGCTTCCG TTGTTCCTAT
    TGCAACAGCA AGCTTAGTCT AGGAACTTAT GCCTCTTTAC ATGGGAGAAT CTATTGCAAG
    CCTCACTTCA ATCAACTCTT TAAATCTAAA GGTAACTATG ATGAAGGCTT TGGACACAGA
    CCACACAAGG ATCTGTGGGC AAGCAAAAAT GAAAATGAAG AGACATTGGA GAGACCAGTC
    CAGCTTCCAA GTGCAGCGGA GACCCTTCAG AGCCCAGGGG TAGAAAATGC CCCCATTGAG
    AAAGTGGGTG TGCTAACTGC GAGTATGGAA GCCAAGGCCT CCTGCCTACT GGAGAAGGAA
    GACAAGCCAG CTGAAACCAA GAAGCTGAGG ATCGCCTGGC CACCCCCCAC TGAGCTTGGC
    AGTTCAGGAA GTGCCTTGGA AGAAGGGATC AAAGTATTGA AGCCTAAGTG GCCTCCTGAG
    GATGATATTA GCAAGCCTGA AGCTCCTGAG GATGTAGACC TGGATCTGAA GAAGCTAAGA
    CGATCTTCTT CTCTGAAGGA GAGAAGCCGC CCATTCACCG TAGCAGCTTC ATTTCGAACC
    TCTTCAGTCA AGAGCCCAAA AGCTTTAAAC CCACCTATCA GGAAGGGCTG GAGCATGTCA
    GAGCAAAGCG AGGAGTATGG AGGAGGTGTA GCAGCAGAAA GGAAACACAT GGAAAATGCC
    AACGCCTCTG AGAAGAATGA GAGTGTGGGG AAAACCACCT GGCCCAACAA GGGATCTAAA
    GGAGGGGAGG CTGGGCGGAG AAGCAGGGAG AGTCCTAGTT TTGGAATGGG GAGTGAAAAT
    CTTATAGAGA ATGGTGCAAA CTTAGATGAA AGTGATAAAA ACCTTCTCAA AAAGCAGTCT
    CCACTAGAAC CCAAGGCAAA AACCTGGTCT AGCTATACAG ACAACCCCTC CACTAAAGAA
    TTCACTACTC AAACTCAGAA ATCCCAGGAT GTGGGATTCT GGGAGGAAGA GGTAGTCGAA
    GAGCTGTCTG TCGAAGAACA GATAAAGAGA AATCGGTACT ATGATGAGGA TGAAGATGAA
    GAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005206381.1
    CDS146..2431
    Translation

    Target ORF information:

    RefSeq Version XM_005206324.3
    Organism Bos taurus(cattle)
    Definition Bos taurus LIM domain and actin binding 1 (LIMA1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005206324.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGAACCAA CTCCATTTAA CAGACGGCAA TGGGCTTCTT TATCGTTGAG GGTTACAGCC 
    AAAGAACTTT CCCTTGTCAA CAAGAACAAC TCATCGGCCA TTGTAGAAAT ATTCTCCAAG
    TACCAGAAAG CAGCGGAAGA AGCAAACATG GAAAAGAAAA GAAGTAACGC TGAAAATCTC
    CCCCAGCACT TTAGAAGGGG GAACCTGACT GCGTTAAGGA AGAAGTGGGA GAACCCCGTG
    CTGGGAGCCG ACTCTCTCCC AGACTCAGTG CGAAACAGCA GCGCAGAGGT CAGGCACAGA
    GGCGACCCTC CTCCCGCTGA AGTGGTGGGC AGCTCTGCCT CTGGGGTCCA GGCTGACCGA
    GAGGAGCTAG TCCAACCCAG ACCTAAGATC AGATCGCCTC CTGAAGCTCC TACTAAGTAC
    CCGCACCCCC GCATCAAGGA CAGTGAGCAT CTTAAAGACC ACTCAACAGA AAATAAAAAG
    ATGGAAAACT GTCTGGGAGA ATCCAGACAT GAAGTAGCAA AACCCGAAAT GAGTGAAAAC
    GCAGAAACTG CAAACAAAAT AGAGAAATAC AATGTTCCGC TGAACAGGCT GAAGATGATG
    TTTGAGAAAG GTGAACCAAC TCAAACCAAG ATTCCTCGGG GCCAAAGCCG AAGTGCAGGT
    GGAAGGAAGA TGTCTGAAAA TAGCTATTCT CTGGATGACT TGGAAATAGG CCCAGGTCAG
    CTGTCATCTT CAGCACTCAA CTCAGAGAAA AGAGAGAGTA GGCGAAATCT AGAATTTCCA
    CGCCTGTCAG AAACCTCCAT AAAGGATCGA ATGGCCAAGT ACCAGGCAGC TGTGTCCAAG
    CAAAGCAGTT CTTCGAGCTA TACAAATGAG TTGAGAGCCA GTGGTGGTGA AATCAAAACT
    CATAAATTGG AGCAAAAGGA GAATGTGCCC CCAGGTCCTG AGGTCTGCAT CTCCTACCCA
    GATGGAGAAA AGGTTTCTGC ATCTGAGAAT AGCCTGGCAG CCCATTCCAC CCTTGCTGAA
    GATGATTCCC CAGGTAACTC CCAGGTCAAG AGTGAGGTCC AGCAGCCTGT GCATCCCAAG
    CCATTAAGTC CAGATGCCAG AGCCTCCAGC CTTTCTGAAA GTTCTCCTCC CAAAGCAGTG
    AAGAAGTTTC AGGCACCCGC AAGAGAGACC TGCGTGGAAT GTCAGAAAAC AGTCTACTCG
    ATGGAGCGTC TCTTGGCCAA CCAGCAGGTG TTTCACATCA GCTGCTTCCG TTGTTCCTAT
    TGCAACAGCA AGCTTAGTCT AGGAACTTAT GCCTCTTTAC ATGGGAGAAT CTATTGCAAG
    CCTCACTTCA ATCAACTCTT TAAATCTAAA GGTAACTATG ATGAAGGCTT TGGACACAGA
    CCACACAAGG ATCTGTGGGC AAGCAAAAAT GAAAATGAAG AGACATTGGA GAGACCAGTC
    CAGCTTCCAA GTGCAGCGGA GACCCTTCAG AGCCCAGGGG TAGAAAATGC CCCCATTGAG
    AAAGTGGGTG TGCTAACTGC GAGTATGGAA GCCAAGGCCT CCTGCCTACT GGAGAAGGAA
    GACAAGCCAG CTGAAACCAA GAAGCTGAGG ATCGCCTGGC CACCCCCCAC TGAGCTTGGC
    AGTTCAGGAA GTGCCTTGGA AGAAGGGATC AAAGTATTGA AGCCTAAGTG GCCTCCTGAG
    GATGATATTA GCAAGCCTGA AGCTCCTGAG GATGTAGACC TGGATCTGAA GAAGCTAAGA
    CGATCTTCTT CTCTGAAGGA GAGAAGCCGC CCATTCACCG TAGCAGCTTC ATTTCGAACC
    TCTTCAGTCA AGAGCCCAAA AGCTTTAAAC CCACCTATCA GGAAGGGCTG GAGCATGTCA
    GAGCAAAGCG AGGAGTATGG AGGAGGTGTA GCAGCAGAAA GGAAACACAT GGAAAATGCC
    AACGCCTCTG AGAAGAATGA GAGTGTGGGG AAAACCACCT GGCCCAACAA GGGATCTAAA
    GGAGGGGAGG CTGGGCGGAG AAGCAGGGAG AGTCCTAGTT TTGGAATGGG GAGTGAAAAT
    CTTATAGAGA ATGGTGCAAA CTTAGATGAA AGTGATAAAA ACCTTCTCAA AAAGCAGTCT
    CCACTAGAAC CCAAGGCAAA AACCTGGTCT AGCTATACAG ACAACCCCTC CACTAAAGAA
    TTCACTACTC AAACTCAGAA ATCCCAGGAT GTGGGATTCT GGGAGGAAGA GGTAGTCGAA
    GAGCTGTCTG TCGAAGAACA GATAAAGAGA AATCGGTACT ATGATGAGGA TGAAGATGAA
    GAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa19235
    Clone ID Related Accession (Same CDS sequence) XM_005206325.4 , XM_005206325.3
    Accession Version XM_005206325.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2283bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product LIM domain and actin-binding protein 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005206325.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGAACCAA CTCCATTTAA CAGACGGCAA TGGGCTTCTT TATCGTTGAG GGTTACAGCC 
    AAAGAACTTT CCCTTGTCAA CAAGAACAAC TCATCGGCCA TTGTAGAAAT ATTCTCCAAG
    TACCAGAAAG CAGCGGAAGA AGCAAACATG GAAAAGAAAA GAAGTAACGC TGAAAATCTC
    CCCCAGCACT TTAGAAGGGG GAACCTGACT GCGTTAAGGA AGAAGTGGGA GAACCCCGTG
    CTGGGAGCCG ACTCTCTCCC AGACTCAGTG CGAAACAGCA GCGCAGAGGT CAGGCACAGA
    GGCGACCCTC CTCCCGCTGA AGTGGTGGGC AGCTCTGCCT CTGGGGTCCA GGCTGACCGA
    GAGGAGCTAG TCCAACCCAG ACCTAAGATC AGATCGCCTC CTGAAGCTCC TACTAAGTAC
    CCGCACCCCC GCATCAAGGA CAGTGAGCAT CTTAAAGACC ACTCAACAGA AAATAAAAAG
    ATGGAAAACT GTCTGGGAGA ATCCAGACAT GAAGTAGCAA AACCCGAAAT GAGTGAAAAC
    GCAGAAACTG CAAACAAAAT AGAGAAATAC AATGTTCCGC TGAACAGGCT GAAGATGATG
    TTTGAGAAAG GTGAACCAAC TCAAACCAAG ATTCCTCGGG GCCAAAGCCG AAGTGCAGGT
    GGAAGGAAGA TGTCTGAAAA TAGCTATTCT CTGGATGACT TGGAAATAGG CCCAGGTCAG
    CTGTCATCTT CAGCACTCAA CTCAGAGAAA AGAGAGAGTA GGCGAAATCT AGAATTTCCA
    CGCCTGTCAG AAACCTCCAT AAAGGATCGA ATGGCCAAGT ACCAGGCAGC TGTGTCCAAG
    CAAAGCAGTT CTTCGAGCTA TACAAATGAG TTGAGAGCCA GTGGTGGTGA AATCAAAACT
    CATAAATTGG AGCAAAAGGA GAATGTGCCC CCAGGTCCTG AGGTCTGCAT CTCCTACCCA
    GATGGAGAAA AGGTTTCTGC ATCTGAGAAT AGCCTGGCAG CCCATTCCAC CCTTGCTGAA
    GATGATTCCC GTAACTCCCA GGTCAAGAGT GAGGTCCAGC AGCCTGTGCA TCCCAAGCCA
    TTAAGTCCAG ATGCCAGAGC CTCCAGCCTT TCTGAAAGTT CTCCTCCCAA AGCAGTGAAG
    AAGTTTCAGG CACCCGCAAG AGAGACCTGC GTGGAATGTC AGAAAACAGT CTACTCGATG
    GAGCGTCTCT TGGCCAACCA GCAGGTGTTT CACATCAGCT GCTTCCGTTG TTCCTATTGC
    AACAGCAAGC TTAGTCTAGG AACTTATGCC TCTTTACATG GGAGAATCTA TTGCAAGCCT
    CACTTCAATC AACTCTTTAA ATCTAAAGGT AACTATGATG AAGGCTTTGG ACACAGACCA
    CACAAGGATC TGTGGGCAAG CAAAAATGAA AATGAAGAGA CATTGGAGAG ACCAGTCCAG
    CTTCCAAGTG CAGCGGAGAC CCTTCAGAGC CCAGGGGTAG AAAATGCCCC CATTGAGAAA
    GTGGGTGTGC TAACTGCGAG TATGGAAGCC AAGGCCTCCT GCCTACTGGA GAAGGAAGAC
    AAGCCAGCTG AAACCAAGAA GCTGAGGATC GCCTGGCCAC CCCCCACTGA GCTTGGCAGT
    TCAGGAAGTG CCTTGGAAGA AGGGATCAAA GTATTGAAGC CTAAGTGGCC TCCTGAGGAT
    GATATTAGCA AGCCTGAAGC TCCTGAGGAT GTAGACCTGG ATCTGAAGAA GCTAAGACGA
    TCTTCTTCTC TGAAGGAGAG AAGCCGCCCA TTCACCGTAG CAGCTTCATT TCGAACCTCT
    TCAGTCAAGA GCCCAAAAGC TTTAAACCCA CCTATCAGGA AGGGCTGGAG CATGTCAGAG
    CAAAGCGAGG AGTATGGAGG AGGTGTAGCA GCAGAAAGGA AACACATGGA AAATGCCAAC
    GCCTCTGAGA AGAATGAGAG TGTGGGGAAA ACCACCTGGC CCAACAAGGG ATCTAAAGGA
    GGGGAGGCTG GGCGGAGAAG CAGGGAGAGT CCTAGTTTTG GAATGGGGAG TGAAAATCTT
    ATAGAGAATG GTGCAAACTT AGATGAAAGT GATAAAAACC TTCTCAAAAA GCAGTCTCCA
    CTAGAACCCA AGGCAAAAAC CTGGTCTAGC TATACAGACA ACCCCTCCAC TAAAGAATTC
    ACTACTCAAA CTCAGAAATC CCAGGATGTG GGATTCTGGG AGGAAGAGGT AGTCGAAGAG
    CTGTCTGTCG AAGAACAGAT AAAGAGAAAT CGGTACTATG ATGAGGATGA AGATGAAGAA
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005206382.1
    CDS192..2474
    Translation

    Target ORF information:

    RefSeq Version XM_005206325.4
    Organism Bos taurus(cattle)
    Definition Bos taurus LIM domain and actin binding 1 (LIMA1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005206325.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGAACCAA CTCCATTTAA CAGACGGCAA TGGGCTTCTT TATCGTTGAG GGTTACAGCC 
    AAAGAACTTT CCCTTGTCAA CAAGAACAAC TCATCGGCCA TTGTAGAAAT ATTCTCCAAG
    TACCAGAAAG CAGCGGAAGA AGCAAACATG GAAAAGAAAA GAAGTAACGC TGAAAATCTC
    CCCCAGCACT TTAGAAGGGG GAACCTGACT GCGTTAAGGA AGAAGTGGGA GAACCCCGTG
    CTGGGAGCCG ACTCTCTCCC AGACTCAGTG CGAAACAGCA GCGCAGAGGT CAGGCACAGA
    GGCGACCCTC CTCCCGCTGA AGTGGTGGGC AGCTCTGCCT CTGGGGTCCA GGCTGACCGA
    GAGGAGCTAG TCCAACCCAG ACCTAAGATC AGATCGCCTC CTGAAGCTCC TACTAAGTAC
    CCGCACCCCC GCATCAAGGA CAGTGAGCAT CTTAAAGACC ACTCAACAGA AAATAAAAAG
    ATGGAAAACT GTCTGGGAGA ATCCAGACAT GAAGTAGCAA AACCCGAAAT GAGTGAAAAC
    GCAGAAACTG CAAACAAAAT AGAGAAATAC AATGTTCCGC TGAACAGGCT GAAGATGATG
    TTTGAGAAAG GTGAACCAAC TCAAACCAAG ATTCCTCGGG GCCAAAGCCG AAGTGCAGGT
    GGAAGGAAGA TGTCTGAAAA TAGCTATTCT CTGGATGACT TGGAAATAGG CCCAGGTCAG
    CTGTCATCTT CAGCACTCAA CTCAGAGAAA AGAGAGAGTA GGCGAAATCT AGAATTTCCA
    CGCCTGTCAG AAACCTCCAT AAAGGATCGA ATGGCCAAGT ACCAGGCAGC TGTGTCCAAG
    CAAAGCAGTT CTTCGAGCTA TACAAATGAG TTGAGAGCCA GTGGTGGTGA AATCAAAACT
    CATAAATTGG AGCAAAAGGA GAATGTGCCC CCAGGTCCTG AGGTCTGCAT CTCCTACCCA
    GATGGAGAAA AGGTTTCTGC ATCTGAGAAT AGCCTGGCAG CCCATTCCAC CCTTGCTGAA
    GATGATTCCC GTAACTCCCA GGTCAAGAGT GAGGTCCAGC AGCCTGTGCA TCCCAAGCCA
    TTAAGTCCAG ATGCCAGAGC CTCCAGCCTT TCTGAAAGTT CTCCTCCCAA AGCAGTGAAG
    AAGTTTCAGG CACCCGCAAG AGAGACCTGC GTGGAATGTC AGAAAACAGT CTACTCGATG
    GAGCGTCTCT TGGCCAACCA GCAGGTGTTT CACATCAGCT GCTTCCGTTG TTCCTATTGC
    AACAGCAAGC TTAGTCTAGG AACTTATGCC TCTTTACATG GGAGAATCTA TTGCAAGCCT
    CACTTCAATC AACTCTTTAA ATCTAAAGGT AACTATGATG AAGGCTTTGG ACACAGACCA
    CACAAGGATC TGTGGGCAAG CAAAAATGAA AATGAAGAGA CATTGGAGAG ACCAGTCCAG
    CTTCCAAGTG CAGCGGAGAC CCTTCAGAGC CCAGGGGTAG AAAATGCCCC CATTGAGAAA
    GTGGGTGTGC TAACTGCGAG TATGGAAGCC AAGGCCTCCT GCCTACTGGA GAAGGAAGAC
    AAGCCAGCTG AAACCAAGAA GCTGAGGATC GCCTGGCCAC CCCCCACTGA GCTTGGCAGT
    TCAGGAAGTG CCTTGGAAGA AGGGATCAAA GTATTGAAGC CTAAGTGGCC TCCTGAGGAT
    GATATTAGCA AGCCTGAAGC TCCTGAGGAT GTAGACCTGG ATCTGAAGAA GCTAAGACGA
    TCTTCTTCTC TGAAGGAGAG AAGCCGCCCA TTCACCGTAG CAGCTTCATT TCGAACCTCT
    TCAGTCAAGA GCCCAAAAGC TTTAAACCCA CCTATCAGGA AGGGCTGGAG CATGTCAGAG
    CAAAGCGAGG AGTATGGAGG AGGTGTAGCA GCAGAAAGGA AACACATGGA AAATGCCAAC
    GCCTCTGAGA AGAATGAGAG TGTGGGGAAA ACCACCTGGC CCAACAAGGG ATCTAAAGGA
    GGGGAGGCTG GGCGGAGAAG CAGGGAGAGT CCTAGTTTTG GAATGGGGAG TGAAAATCTT
    ATAGAGAATG GTGCAAACTT AGATGAAAGT GATAAAAACC TTCTCAAAAA GCAGTCTCCA
    CTAGAACCCA AGGCAAAAAC CTGGTCTAGC TATACAGACA ACCCCTCCAC TAAAGAATTC
    ACTACTCAAA CTCAGAAATC CCAGGATGTG GGATTCTGGG AGGAAGAGGT AGTCGAAGAG
    CTGTCTGTCG AAGAACAGAT AAAGAGAAAT CGGTACTATG ATGAGGATGA AGATGAAGAA
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa19235
    Clone ID Related Accession (Same CDS sequence) XM_005206325.4 , XM_005206325.3
    Accession Version XM_005206325.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2283bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product LIM domain and actin-binding protein 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103920.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005206325.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGAACCAA CTCCATTTAA CAGACGGCAA TGGGCTTCTT TATCGTTGAG GGTTACAGCC 
    AAAGAACTTT CCCTTGTCAA CAAGAACAAC TCATCGGCCA TTGTAGAAAT ATTCTCCAAG
    TACCAGAAAG CAGCGGAAGA AGCAAACATG GAAAAGAAAA GAAGTAACGC TGAAAATCTC
    CCCCAGCACT TTAGAAGGGG GAACCTGACT GCGTTAAGGA AGAAGTGGGA GAACCCCGTG
    CTGGGAGCCG ACTCTCTCCC AGACTCAGTG CGAAACAGCA GCGCAGAGGT CAGGCACAGA
    GGCGACCCTC CTCCCGCTGA AGTGGTGGGC AGCTCTGCCT CTGGGGTCCA GGCTGACCGA
    GAGGAGCTAG TCCAACCCAG ACCTAAGATC AGATCGCCTC CTGAAGCTCC TACTAAGTAC
    CCGCACCCCC GCATCAAGGA CAGTGAGCAT CTTAAAGACC ACTCAACAGA AAATAAAAAG
    ATGGAAAACT GTCTGGGAGA ATCCAGACAT GAAGTAGCAA AACCCGAAAT GAGTGAAAAC
    GCAGAAACTG CAAACAAAAT AGAGAAATAC AATGTTCCGC TGAACAGGCT GAAGATGATG
    TTTGAGAAAG GTGAACCAAC TCAAACCAAG ATTCCTCGGG GCCAAAGCCG AAGTGCAGGT
    GGAAGGAAGA TGTCTGAAAA TAGCTATTCT CTGGATGACT TGGAAATAGG CCCAGGTCAG
    CTGTCATCTT CAGCACTCAA CTCAGAGAAA AGAGAGAGTA GGCGAAATCT AGAATTTCCA
    CGCCTGTCAG AAACCTCCAT AAAGGATCGA ATGGCCAAGT ACCAGGCAGC TGTGTCCAAG
    CAAAGCAGTT CTTCGAGCTA TACAAATGAG TTGAGAGCCA GTGGTGGTGA AATCAAAACT
    CATAAATTGG AGCAAAAGGA GAATGTGCCC CCAGGTCCTG AGGTCTGCAT CTCCTACCCA
    GATGGAGAAA AGGTTTCTGC ATCTGAGAAT AGCCTGGCAG CCCATTCCAC CCTTGCTGAA
    GATGATTCCC GTAACTCCCA GGTCAAGAGT GAGGTCCAGC AGCCTGTGCA TCCCAAGCCA
    TTAAGTCCAG ATGCCAGAGC CTCCAGCCTT TCTGAAAGTT CTCCTCCCAA AGCAGTGAAG
    AAGTTTCAGG CACCCGCAAG AGAGACCTGC GTGGAATGTC AGAAAACAGT CTACTCGATG
    GAGCGTCTCT TGGCCAACCA GCAGGTGTTT CACATCAGCT GCTTCCGTTG TTCCTATTGC
    AACAGCAAGC TTAGTCTAGG AACTTATGCC TCTTTACATG GGAGAATCTA TTGCAAGCCT
    CACTTCAATC AACTCTTTAA ATCTAAAGGT AACTATGATG AAGGCTTTGG ACACAGACCA
    CACAAGGATC TGTGGGCAAG CAAAAATGAA AATGAAGAGA CATTGGAGAG ACCAGTCCAG
    CTTCCAAGTG CAGCGGAGAC CCTTCAGAGC CCAGGGGTAG AAAATGCCCC CATTGAGAAA
    GTGGGTGTGC TAACTGCGAG TATGGAAGCC AAGGCCTCCT GCCTACTGGA GAAGGAAGAC
    AAGCCAGCTG AAACCAAGAA GCTGAGGATC GCCTGGCCAC CCCCCACTGA GCTTGGCAGT
    TCAGGAAGTG CCTTGGAAGA AGGGATCAAA GTATTGAAGC CTAAGTGGCC TCCTGAGGAT
    GATATTAGCA AGCCTGAAGC TCCTGAGGAT GTAGACCTGG ATCTGAAGAA GCTAAGACGA
    TCTTCTTCTC TGAAGGAGAG AAGCCGCCCA TTCACCGTAG CAGCTTCATT TCGAACCTCT
    TCAGTCAAGA GCCCAAAAGC TTTAAACCCA CCTATCAGGA AGGGCTGGAG CATGTCAGAG
    CAAAGCGAGG AGTATGGAGG AGGTGTAGCA GCAGAAAGGA AACACATGGA AAATGCCAAC
    GCCTCTGAGA AGAATGAGAG TGTGGGGAAA ACCACCTGGC CCAACAAGGG ATCTAAAGGA
    GGGGAGGCTG GGCGGAGAAG CAGGGAGAGT CCTAGTTTTG GAATGGGGAG TGAAAATCTT
    ATAGAGAATG GTGCAAACTT AGATGAAAGT GATAAAAACC TTCTCAAAAA GCAGTCTCCA
    CTAGAACCCA AGGCAAAAAC CTGGTCTAGC TATACAGACA ACCCCTCCAC TAAAGAATTC
    ACTACTCAAA CTCAGAAATC CCAGGATGTG GGATTCTGGG AGGAAGAGGT AGTCGAAGAG
    CTGTCTGTCG AAGAACAGAT AAAGAGAAAT CGGTACTATG ATGAGGATGA AGATGAAGAA
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005206382.1
    CDS146..2428
    Translation

    Target ORF information:

    RefSeq Version XM_005206325.3
    Organism Bos taurus(cattle)
    Definition Bos taurus LIM domain and actin binding 1 (LIMA1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005206325.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGAACCAA CTCCATTTAA CAGACGGCAA TGGGCTTCTT TATCGTTGAG GGTTACAGCC 
    AAAGAACTTT CCCTTGTCAA CAAGAACAAC TCATCGGCCA TTGTAGAAAT ATTCTCCAAG
    TACCAGAAAG CAGCGGAAGA AGCAAACATG GAAAAGAAAA GAAGTAACGC TGAAAATCTC
    CCCCAGCACT TTAGAAGGGG GAACCTGACT GCGTTAAGGA AGAAGTGGGA GAACCCCGTG
    CTGGGAGCCG ACTCTCTCCC AGACTCAGTG CGAAACAGCA GCGCAGAGGT CAGGCACAGA
    GGCGACCCTC CTCCCGCTGA AGTGGTGGGC AGCTCTGCCT CTGGGGTCCA GGCTGACCGA
    GAGGAGCTAG TCCAACCCAG ACCTAAGATC AGATCGCCTC CTGAAGCTCC TACTAAGTAC
    CCGCACCCCC GCATCAAGGA CAGTGAGCAT CTTAAAGACC ACTCAACAGA AAATAAAAAG
    ATGGAAAACT GTCTGGGAGA ATCCAGACAT GAAGTAGCAA AACCCGAAAT GAGTGAAAAC
    GCAGAAACTG CAAACAAAAT AGAGAAATAC AATGTTCCGC TGAACAGGCT GAAGATGATG
    TTTGAGAAAG GTGAACCAAC TCAAACCAAG ATTCCTCGGG GCCAAAGCCG AAGTGCAGGT
    GGAAGGAAGA TGTCTGAAAA TAGCTATTCT CTGGATGACT TGGAAATAGG CCCAGGTCAG
    CTGTCATCTT CAGCACTCAA CTCAGAGAAA AGAGAGAGTA GGCGAAATCT AGAATTTCCA
    CGCCTGTCAG AAACCTCCAT AAAGGATCGA ATGGCCAAGT ACCAGGCAGC TGTGTCCAAG
    CAAAGCAGTT CTTCGAGCTA TACAAATGAG TTGAGAGCCA GTGGTGGTGA AATCAAAACT
    CATAAATTGG AGCAAAAGGA GAATGTGCCC CCAGGTCCTG AGGTCTGCAT CTCCTACCCA
    GATGGAGAAA AGGTTTCTGC ATCTGAGAAT AGCCTGGCAG CCCATTCCAC CCTTGCTGAA
    GATGATTCCC GTAACTCCCA GGTCAAGAGT GAGGTCCAGC AGCCTGTGCA TCCCAAGCCA
    TTAAGTCCAG ATGCCAGAGC CTCCAGCCTT TCTGAAAGTT CTCCTCCCAA AGCAGTGAAG
    AAGTTTCAGG CACCCGCAAG AGAGACCTGC GTGGAATGTC AGAAAACAGT CTACTCGATG
    GAGCGTCTCT TGGCCAACCA GCAGGTGTTT CACATCAGCT GCTTCCGTTG TTCCTATTGC
    AACAGCAAGC TTAGTCTAGG AACTTATGCC TCTTTACATG GGAGAATCTA TTGCAAGCCT
    CACTTCAATC AACTCTTTAA ATCTAAAGGT AACTATGATG AAGGCTTTGG ACACAGACCA
    CACAAGGATC TGTGGGCAAG CAAAAATGAA AATGAAGAGA CATTGGAGAG ACCAGTCCAG
    CTTCCAAGTG CAGCGGAGAC CCTTCAGAGC CCAGGGGTAG AAAATGCCCC CATTGAGAAA
    GTGGGTGTGC TAACTGCGAG TATGGAAGCC AAGGCCTCCT GCCTACTGGA GAAGGAAGAC
    AAGCCAGCTG AAACCAAGAA GCTGAGGATC GCCTGGCCAC CCCCCACTGA GCTTGGCAGT
    TCAGGAAGTG CCTTGGAAGA AGGGATCAAA GTATTGAAGC CTAAGTGGCC TCCTGAGGAT
    GATATTAGCA AGCCTGAAGC TCCTGAGGAT GTAGACCTGG ATCTGAAGAA GCTAAGACGA
    TCTTCTTCTC TGAAGGAGAG AAGCCGCCCA TTCACCGTAG CAGCTTCATT TCGAACCTCT
    TCAGTCAAGA GCCCAAAAGC TTTAAACCCA CCTATCAGGA AGGGCTGGAG CATGTCAGAG
    CAAAGCGAGG AGTATGGAGG AGGTGTAGCA GCAGAAAGGA AACACATGGA AAATGCCAAC
    GCCTCTGAGA AGAATGAGAG TGTGGGGAAA ACCACCTGGC CCAACAAGGG ATCTAAAGGA
    GGGGAGGCTG GGCGGAGAAG CAGGGAGAGT CCTAGTTTTG GAATGGGGAG TGAAAATCTT
    ATAGAGAATG GTGCAAACTT AGATGAAAGT GATAAAAACC TTCTCAAAAA GCAGTCTCCA
    CTAGAACCCA AGGCAAAAAC CTGGTCTAGC TATACAGACA ACCCCTCCAC TAAAGAATTC
    ACTACTCAAA CTCAGAAATC CCAGGATGTG GGATTCTGGG AGGAAGAGGT AGTCGAAGAG
    CTGTCTGTCG AAGAACAGAT AAAGAGAAAT CGGTACTATG ATGAGGATGA AGATGAAGAA
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa54749
    Clone ID Related Accession (Same CDS sequence) XM_010804991.2
    Accession Version XM_010804991.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2136bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product LIM domain and actin-binding protein 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103920.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010804991.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGAGGGCA AGAAGAACGC TGAAAATCTC CCCCAGCACT TTAGAAGGGG GAACCTGACT 
    GCGTTAAGGA AGAAGTGGGA GAACCCCGTG CTGGGAGCCG ACTCTCTCCC AGACTCAGTG
    CGAAACAGCA GCGCAGAGGT CAGGCACAGA GGCGACCCTC CTCCCGCTGA AGTGGTGGGC
    AGCTCTGCCT CTGGGGTCCA GGCTGACCGA GAGGAGCTAG TCCAACCCAG ACCTAAGATC
    AGATCGCCTC CTGAAGCTCC TACTAAGTAC CCGCACCCCC GCATCAAGGA CAGTGAGCAT
    CTTAAAGACC ACTCAACAGA AAATAAAAAG ATGGAAAACT GTCTGGGAGA ATCCAGACAT
    GAAGTAGCAA AACCCGAAAT GAGTGAAAAC GCAGAAACTG CAAACAAAAT AGAGAAATAC
    AATGTTCCGC TGAACAGGCT GAAGATGATG TTTGAGAAAG GTGAACCAAC TCAAACCAAG
    ATTCCTCGGG GCCAAAGCCG AAGTGCAGGT GGAAGGAAGA TGTCTGAAAA TAGCTATTCT
    CTGGATGACT TGGAAATAGG CCCAGGTCAG CTGTCATCTT CAGCACTCAA CTCAGAGAAA
    AGAGAGAGTA GGCGAAATCT AGAATTTCCA CGCCTGTCAG AAACCTCCAT AAAGGATCGA
    ATGGCCAAGT ACCAGGCAGC TGTGTCCAAG CAAAGCAGTT CTTCGAGCTA TACAAATGAG
    TTGAGAGCCA GTGGTGGTGA AATCAAAACT CATAAATTGG AGCAAAAGGA GAATGTGCCC
    CCAGGTCCTG AGGTCTGCAT CTCCTACCCA GATGGAGAAA AGGTTTCTGC ATCTGAGAAT
    AGCCTGGCAG CCCATTCCAC CCTTGCTGAA GATGATTCCC CAGGTAACTC CCAGGTCAAG
    AGTGAGGTCC AGCAGCCTGT GCATCCCAAG CCATTAAGTC CAGATGCCAG AGCCTCCAGC
    CTTTCTGAAA GTTCTCCTCC CAAAGCAGTG AAGAAGTTTC AGGCACCCGC AAGAGAGACC
    TGCGTGGAAT GTCAGAAAAC AGTCTACTCG ATGGAGCGTC TCTTGGCCAA CCAGCAGGTG
    TTTCACATCA GCTGCTTCCG TTGTTCCTAT TGCAACAGCA AGCTTAGTCT AGGAACTTAT
    GCCTCTTTAC ATGGGAGAAT CTATTGCAAG CCTCACTTCA ATCAACTCTT TAAATCTAAA
    GGTAACTATG ATGAAGGCTT TGGACACAGA CCACACAAGG ATCTGTGGGC AAGCAAAAAT
    GAAAATGAAG AGACATTGGA GAGACCAGTC CAGCTTCCAA GTGCAGCGGA GACCCTTCAG
    AGCCCAGGGG TAGAAAATGC CCCCATTGAG AAAGTGGGTG TGCTAACTGC GAGTATGGAA
    GCCAAGGCCT CCTGCCTACT GGAGAAGGAA GACAAGCCAG CTGAAACCAA GAAGCTGAGG
    ATCGCCTGGC CACCCCCCAC TGAGCTTGGC AGTTCAGGAA GTGCCTTGGA AGAAGGGATC
    AAAGTATTGA AGCCTAAGTG GCCTCCTGAG GATGATATTA GCAAGCCTGA AGCTCCTGAG
    GATGTAGACC TGGATCTGAA GAAGCTAAGA CGATCTTCTT CTCTGAAGGA GAGAAGCCGC
    CCATTCACCG TAGCAGCTTC ATTTCGAACC TCTTCAGTCA AGAGCCCAAA AGCTTTAAAC
    CCACCTATCA GGAAGGGCTG GAGCATGTCA GAGCAAAGCG AGGAGTATGG AGGAGGTGTA
    GCAGCAGAAA GGAAACACAT GGAAAATGCC AACGCCTCTG AGAAGAATGA GAGTGTGGGG
    AAAACCACCT GGCCCAACAA GGGATCTAAA GGAGGGGAGG CTGGGCGGAG AAGCAGGGAG
    AGTCCTAGTT TTGGAATGGG GAGTGAAAAT CTTATAGAGA ATGGTGCAAA CTTAGATGAA
    AGTGATAAAA ACCTTCTCAA AAAGCAGTCT CCACTAGAAC CCAAGGCAAA AACCTGGTCT
    AGCTATACAG ACAACCCCTC CACTAAAGAA TTCACTACTC AAACTCAGAA ATCCCAGGAT
    GTGGGATTCT GGGAGGAAGA GGTAGTCGAA GAGCTGTCTG TCGAAGAACA GATAAAGAGA
    AATCGGTACT ATGATGAGGA TGAAGATGAA GAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010803293.1
    CDS5..2140
    Translation

    Target ORF information:

    RefSeq Version XM_010804991.2
    Organism Bos taurus(cattle)
    Definition Bos taurus LIM domain and actin binding 1 (LIMA1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010804991.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGAGGGCA AGAAGAACGC TGAAAATCTC CCCCAGCACT TTAGAAGGGG GAACCTGACT 
    GCGTTAAGGA AGAAGTGGGA GAACCCCGTG CTGGGAGCCG ACTCTCTCCC AGACTCAGTG
    CGAAACAGCA GCGCAGAGGT CAGGCACAGA GGCGACCCTC CTCCCGCTGA AGTGGTGGGC
    AGCTCTGCCT CTGGGGTCCA GGCTGACCGA GAGGAGCTAG TCCAACCCAG ACCTAAGATC
    AGATCGCCTC CTGAAGCTCC TACTAAGTAC CCGCACCCCC GCATCAAGGA CAGTGAGCAT
    CTTAAAGACC ACTCAACAGA AAATAAAAAG ATGGAAAACT GTCTGGGAGA ATCCAGACAT
    GAAGTAGCAA AACCCGAAAT GAGTGAAAAC GCAGAAACTG CAAACAAAAT AGAGAAATAC
    AATGTTCCGC TGAACAGGCT GAAGATGATG TTTGAGAAAG GTGAACCAAC TCAAACCAAG
    ATTCCTCGGG GCCAAAGCCG AAGTGCAGGT GGAAGGAAGA TGTCTGAAAA TAGCTATTCT
    CTGGATGACT TGGAAATAGG CCCAGGTCAG CTGTCATCTT CAGCACTCAA CTCAGAGAAA
    AGAGAGAGTA GGCGAAATCT AGAATTTCCA CGCCTGTCAG AAACCTCCAT AAAGGATCGA
    ATGGCCAAGT ACCAGGCAGC TGTGTCCAAG CAAAGCAGTT CTTCGAGCTA TACAAATGAG
    TTGAGAGCCA GTGGTGGTGA AATCAAAACT CATAAATTGG AGCAAAAGGA GAATGTGCCC
    CCAGGTCCTG AGGTCTGCAT CTCCTACCCA GATGGAGAAA AGGTTTCTGC ATCTGAGAAT
    AGCCTGGCAG CCCATTCCAC CCTTGCTGAA GATGATTCCC CAGGTAACTC CCAGGTCAAG
    AGTGAGGTCC AGCAGCCTGT GCATCCCAAG CCATTAAGTC CAGATGCCAG AGCCTCCAGC
    CTTTCTGAAA GTTCTCCTCC CAAAGCAGTG AAGAAGTTTC AGGCACCCGC AAGAGAGACC
    TGCGTGGAAT GTCAGAAAAC AGTCTACTCG ATGGAGCGTC TCTTGGCCAA CCAGCAGGTG
    TTTCACATCA GCTGCTTCCG TTGTTCCTAT TGCAACAGCA AGCTTAGTCT AGGAACTTAT
    GCCTCTTTAC ATGGGAGAAT CTATTGCAAG CCTCACTTCA ATCAACTCTT TAAATCTAAA
    GGTAACTATG ATGAAGGCTT TGGACACAGA CCACACAAGG ATCTGTGGGC AAGCAAAAAT
    GAAAATGAAG AGACATTGGA GAGACCAGTC CAGCTTCCAA GTGCAGCGGA GACCCTTCAG
    AGCCCAGGGG TAGAAAATGC CCCCATTGAG AAAGTGGGTG TGCTAACTGC GAGTATGGAA
    GCCAAGGCCT CCTGCCTACT GGAGAAGGAA GACAAGCCAG CTGAAACCAA GAAGCTGAGG
    ATCGCCTGGC CACCCCCCAC TGAGCTTGGC AGTTCAGGAA GTGCCTTGGA AGAAGGGATC
    AAAGTATTGA AGCCTAAGTG GCCTCCTGAG GATGATATTA GCAAGCCTGA AGCTCCTGAG
    GATGTAGACC TGGATCTGAA GAAGCTAAGA CGATCTTCTT CTCTGAAGGA GAGAAGCCGC
    CCATTCACCG TAGCAGCTTC ATTTCGAACC TCTTCAGTCA AGAGCCCAAA AGCTTTAAAC
    CCACCTATCA GGAAGGGCTG GAGCATGTCA GAGCAAAGCG AGGAGTATGG AGGAGGTGTA
    GCAGCAGAAA GGAAACACAT GGAAAATGCC AACGCCTCTG AGAAGAATGA GAGTGTGGGG
    AAAACCACCT GGCCCAACAA GGGATCTAAA GGAGGGGAGG CTGGGCGGAG AAGCAGGGAG
    AGTCCTAGTT TTGGAATGGG GAGTGAAAAT CTTATAGAGA ATGGTGCAAA CTTAGATGAA
    AGTGATAAAA ACCTTCTCAA AAAGCAGTCT CCACTAGAAC CCAAGGCAAA AACCTGGTCT
    AGCTATACAG ACAACCCCTC CACTAAAGAA TTCACTACTC AAACTCAGAA ATCCCAGGAT
    GTGGGATTCT GGGAGGAAGA GGTAGTCGAA GAGCTGTCTG TCGAAGAACA GATAAAGAGA
    AATCGGTACT ATGATGAGGA TGAAGATGAA GAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.