LIMA1 gene cDNA ORF clone, Homo sapiens(human)

mRNA	Protein	Name
NM_016357.4	NP_057441.1	LIM domain and actin-binding protein 1 isoform 2
NM_001113547.2	NP_001107019.1	LIM domain and actin-binding protein 1 isoform 3
XM_017019429.1	XP_016874918.1	LIM domain and actin-binding protein 1 isoform X2
NM_001113547.1	NP_001107019.1	LIM domain and actin-binding protein 1 isoform 3
NM_001113546.2	NP_001107018.1	LIM domain and actin-binding protein 1 isoform 1
XM_011538455.2	XP_011536757.1	LIM domain and actin-binding protein 1 isoform X1
NM_001113546.1	NP_001107018.1	LIM domain and actin-binding protein 1 isoform 1
NM_016357.5	NP_057441.1	LIM domain and actin-binding protein 1 isoform 2
NM_001243775.1	NP_001230704.1	LIM domain and actin-binding protein 1 isoform 4
XM_017019430.1	XP_016874919.1	LIM domain and actin-binding protein 1 isoform X3

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu02217	NM_016357.5 Latest version!	Homo sapiens LIM domain and actin binding 1 (LIMA1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu02323	NM_001243775.1 Latest version!	Homo sapiens LIM domain and actin binding 1 (LIMA1), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
OHu18730	NM_001113546.2 Latest version!	Homo sapiens LIM domain and actin binding 1 (LIMA1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu02323	XM_017019430.1 Latest version!	Homo sapiens LIM domain and actin binding 1 (LIMA1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
OHu18730	XM_011538455.2 Latest version!	Homo sapiens LIM domain and actin binding 1 (LIMA1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu06399	NM_001113547.2 Latest version!	Homo sapiens LIM domain and actin binding 1 (LIMA1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$265.30 $379.00
OHu83858	XM_017019429.1 Latest version!	Homo sapiens LIM domain and actin binding 1 (LIMA1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
View Old Accession Versions >>
OHu06399	NM_001113547.1	Homo sapiens LIM domain and actin binding 1 (LIMA1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$265.30 $379.00
OHu18730	NM_001113546.1	Homo sapiens LIM domain and actin binding 1 (LIMA1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu02217	NM_016357.4	Homo sapiens LIM domain and actin binding 1 (LIMA1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
Hide Old Accession Versions >>

LIMA1 ( NM_016357.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_057441.1 Homo sapiens LIM domain and actin binding 1 (LIMA1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu02217
Clone ID Related Accession (Same CDS sequence)	NM_016357.4 , NM_016357.5
Accession Version	NM_016357.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2280bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-03-11
Organism	Homo sapiens(human)
Product	LIM domain and actin-binding protein 1 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP216703.1, BP281423.1, BC110815.1, AL136911.1 and AA772616.1. On Jan 17, 2008 this sequence version replaced NM_016357.3. Transcript Variant: This variant (2) uses an alternate in-frame acceptor splice site at an internal coding exon compared to variant 1. This results in an isoform (2, also known as EPLIN-beta) that is 1 aa shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.6933.1, SRR1803612.72177.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAATCAT CTCCATTTAA TAGACGGCAA TGGACCTCAC TATCATTGAG GGTAACAGCC 
AAAGAACTTT CTCTTGTCAA CAAGAACAAG TCATCGGCTA TTGTGGAAAT ATTCTCCAAG 
TACCAGAAAG CAGCTGAAGA AACAAACATG GAGAAGAAGA GAAGTAACAC CGAAAATCTC 
TCCCAGCACT TTAGAAAGGG GACCCTGACT GTGTTAAAGA AGAAGTGGGA GAACCCAGGG 
CTGGGAGCAG AGTCTCACAC AGACTCTCTA CGGAACAGCA GCACTGAGAT TAGGCACAGA 
GCAGACCATC CTCCTGCTGA AGTGACAAGC CACGCTGCTT CTGGAGCCAA AGCTGACCAA 
GAAGAACAAA TCCACCCCAG ATCTAGACTC AGGTCACCTC CTGAAGCCCT CGTTCAGGGT 
CGATATCCCC ACATCAAGGA CGGTGAGGAT CTTAAAGACC ACTCAACAGA AAGTAAAAAA 
ATGGAAAATT GTCTAGGAGA ATCCAGGCAT GAAGTAGAAA AATCAGAAAT CAGTGAAAAC 
ACAGATGCTT CGGGCAAAAT AGAGAAATAT AATGTTCCGC TGAACAGGCT TAAGATGATG 
TTTGAGAAAG GTGAACCAAC TCAAACTAAG ATTCTCCGGG CCCAAAGCCG AAGTGCAAGT 
GGAAGGAAGA TCTCTGAAAA CAGCTATTCT CTAGATGACC TGGAAATAGG CCCAGGTCAG 
TTGTCATCTT CTACATTTGA CTCGGAGAAA AATGAGAGTA GACGAAATCT GGAACTTCCA 
CGCCTCTCAG AAACCTCTAT AAAGGATCGA ATGGCCAAGT ACCAGGCAGC TGTGTCCAAA 
CAAAGCAGCT CAACCAACTA TACAAATGAG CTGAAAGCCA GTGGTGGCGA AATCAAAATT 
CATAAAATGG AGCAAAAGGA GAATGTGCCC CCAGGTCCTG AGGTCTGCAT CACCCATCAG 
GAAGGGGAAA AGATTTCTGC AAATGAGAAT AGCCTGGCAG TCCGTTCCAC CCCTGCCGAA 
GATGACTCCC GTGACTCCCA GGTTAAGAGT GAGGTTCAAC AGCCTGTCCA TCCCAAGCCA 
CTAAGTCCAG ATTCCAGAGC CTCCAGTCTT TCTGAAAGTT CTCCTCCCAA AGCAATGAAG 
AAGTTTCAGG CACCTGCAAG AGAGACCTGC GTGGAATGTC AGAAGACAGT CTATCCAATG 
GAGCGTCTCT TGGCCAACCA GCAGGTGTTT CACATCAGCT GCTTCCGTTG CTCCTATTGC 
AACAACAAAC TCAGTCTAGG AACATATGCA TCTTTACATG GAAGAATCTA TTGTAAGCCT 
CACTTCAATC AACTCTTTAA ATCTAAGGGC AACTATGATG AAGGCTTTGG GCACAGACCA 
CACAAGGATC TATGGGCAAG CAAAAATGAA AACGAAGAGA TTTTGGAGAG ACCAGCCCAG 
CTTGCAAATG CAAGGGAGAC CCCTCACAGC CCAGGGGTAG AAGATGCCCC TATTGCTAAG 
GTGGGTGTCC TGGCTGCAAG TATGGAAGCC AAGGCCTCCT CTCAGCAGGA GAAGGAAGAC 
AAGCCAGCTG AAACCAAGAA GCTGAGGATC GCCTGGCCAC CCCCCACTGA ACTTGGAAGT 
TCAGGAAGTG CCTTGGAGGA AGGGATCAAA ATGTCAAAGC CCAAATGGCC TCCTGAAGAC 
GAAATCAGCA AGCCCGAAGT TCCTGAGGAT GTCGATCTAG ATCTGAAGAA GCTAAGACGA 
TCTTCTTCAC TGAAGGAAAG AAGCCGCCCA TTCACTGTAG CAGCTTCATT TCAAAGCACC 
TCTGTCAAGA GCCCAAAAAC TGTGTCCCCA CCTATCAGGA AAGGCTGGAG CATGTCAGAG 
CAGAGTGAAG AGTCTGTGGG TGGAAGAGTT GCAGAAAGGA AACAAGTGGA AAATGCCAAG 
GCTTCTAAGA AGAATGGGAA TGTGGGAAAA ACAACCTGGC AAAACAAAGA ATCTAAAGGA 
GAGACAGGGA AGAGAAGTAA GGAAGGTCAT AGTTTGGAGA TGGAGAATGA GAATCTTGTA 
GAAAATGGTG CAGACTCCGA TGAAGATGAT AACAGCTTCC TCAAACAACA ATCTCCACAA 
GAACCCAAGT CTCTGAATTG GTCGAGTTTT GTAGACAACA CCTTTGCTGA AGAATTCACT 
ACTCAGAATC AGAAATCCCA GGATGTGGAA CTCTGGGAGG GAGAAGTGGT CAAAGAGCTC 
TCTGTGGAAG AACAGATAAA GAGAAATCGG TATTATGATG AGGATGAGGA TGAAGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_057441.1
CDS	175..2454
Translation	MESSPFNRRQWTSLSLRVTAKELSLVNKNKSSAIVEIFSKYQKAAEETNMEKKRSNTENLSQHFRKGTLTVLKKKWENPGLGAESHTDSLRNSSTEIRHRADHPPAEVTSHAASGAKADQEEQIHPRSRLRSPPEALVQGRYPHIKDGEDLKDHSTESKKMENCLGESRHEVEKSEISENTDASGKIEKYNVPLNRLKMMFEKGEPTQTKILRAQSRSASGRKISENSYSLDDLEIGPGQLSSSTFDSEKNESRRNLELPRLSETSIKDRMAKYQAAVSKQSSSTNYTNELKASGGEIKIHKMEQKENVPPGPEVCITHQEGEKISANENSLAVRSTPAEDDSRDSQVKSEVQQPVHPKPLSPDSRASSLSESSPPKAMKKFQAPARETCVECQKTVYPMERLLANQQVFHISCFRCSYCNNKLSLGTYASLHGRIYCKPHFNQLFKSKGNYDEGFGHRPHKDLWASKNENEEILERPAQLANARETPHSPGVEDAPIAKVGVLAASMEAKASSQQEKEDKPAETKKLRIAWPPPTELGSSGSALEEGIKMSKPKWPPEDEISKPEVPEDVDLDLKKLRRSSSLKERSRPFTVAASFQSTSVKSPKTVSPPIRKGWSMSEQSEESVGGRVAERKQVENAKASKKNGNVGKTTWQNKESKGETGKRSKEGHSLEMENENLVENGADSDEDDNSFLKQQSPQEPKSLNWSSFVDNTFAEEFTTQNQKSQDVELWEGEVVKELSVEEQIKRNRYYDEDEDEE

Target ORF information:

RefSeq Version	NM_016357.4
Organism	Homo sapiens(human)
Definition	Homo sapiens LIM domain and actin binding 1 (LIMA1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_016357.4

ORF Insert Sequence:

ATGGAATCAT CTCCATTTAA TAGACGGCAA TGGACCTCAC TATCATTGAG GGTAACAGCC 
AAAGAACTTT CTCTTGTCAA CAAGAACAAG TCATCGGCTA TTGTGGAAAT ATTCTCCAAG 
TACCAGAAAG CAGCTGAAGA AACAAACATG GAGAAGAAGA GAAGTAACAC CGAAAATCTC 
TCCCAGCACT TTAGAAAGGG GACCCTGACT GTGTTAAAGA AGAAGTGGGA GAACCCAGGG 
CTGGGAGCAG AGTCTCACAC AGACTCTCTA CGGAACAGCA GCACTGAGAT TAGGCACAGA 
GCAGACCATC CTCCTGCTGA AGTGACAAGC CACGCTGCTT CTGGAGCCAA AGCTGACCAA 
GAAGAACAAA TCCACCCCAG ATCTAGACTC AGGTCACCTC CTGAAGCCCT CGTTCAGGGT 
CGATATCCCC ACATCAAGGA CGGTGAGGAT CTTAAAGACC ACTCAACAGA AAGTAAAAAA 
ATGGAAAATT GTCTAGGAGA ATCCAGGCAT GAAGTAGAAA AATCAGAAAT CAGTGAAAAC 
ACAGATGCTT CGGGCAAAAT AGAGAAATAT AATGTTCCGC TGAACAGGCT TAAGATGATG 
TTTGAGAAAG GTGAACCAAC TCAAACTAAG ATTCTCCGGG CCCAAAGCCG AAGTGCAAGT 
GGAAGGAAGA TCTCTGAAAA CAGCTATTCT CTAGATGACC TGGAAATAGG CCCAGGTCAG 
TTGTCATCTT CTACATTTGA CTCGGAGAAA AATGAGAGTA GACGAAATCT GGAACTTCCA 
CGCCTCTCAG AAACCTCTAT AAAGGATCGA ATGGCCAAGT ACCAGGCAGC TGTGTCCAAA 
CAAAGCAGCT CAACCAACTA TACAAATGAG CTGAAAGCCA GTGGTGGCGA AATCAAAATT 
CATAAAATGG AGCAAAAGGA GAATGTGCCC CCAGGTCCTG AGGTCTGCAT CACCCATCAG 
GAAGGGGAAA AGATTTCTGC AAATGAGAAT AGCCTGGCAG TCCGTTCCAC CCCTGCCGAA 
GATGACTCCC GTGACTCCCA GGTTAAGAGT GAGGTTCAAC AGCCTGTCCA TCCCAAGCCA 
CTAAGTCCAG ATTCCAGAGC CTCCAGTCTT TCTGAAAGTT CTCCTCCCAA AGCAATGAAG 
AAGTTTCAGG CACCTGCAAG AGAGACCTGC GTGGAATGTC AGAAGACAGT CTATCCAATG 
GAGCGTCTCT TGGCCAACCA GCAGGTGTTT CACATCAGCT GCTTCCGTTG CTCCTATTGC 
AACAACAAAC TCAGTCTAGG AACATATGCA TCTTTACATG GAAGAATCTA TTGTAAGCCT 
CACTTCAATC AACTCTTTAA ATCTAAGGGC AACTATGATG AAGGCTTTGG GCACAGACCA 
CACAAGGATC TATGGGCAAG CAAAAATGAA AACGAAGAGA TTTTGGAGAG ACCAGCCCAG 
CTTGCAAATG CAAGGGAGAC CCCTCACAGC CCAGGGGTAG AAGATGCCCC TATTGCTAAG 
GTGGGTGTCC TGGCTGCAAG TATGGAAGCC AAGGCCTCCT CTCAGCAGGA GAAGGAAGAC 
AAGCCAGCTG AAACCAAGAA GCTGAGGATC GCCTGGCCAC CCCCCACTGA ACTTGGAAGT 
TCAGGAAGTG CCTTGGAGGA AGGGATCAAA ATGTCAAAGC CCAAATGGCC TCCTGAAGAC 
GAAATCAGCA AGCCCGAAGT TCCTGAGGAT GTCGATCTAG ATCTGAAGAA GCTAAGACGA 
TCTTCTTCAC TGAAGGAAAG AAGCCGCCCA TTCACTGTAG CAGCTTCATT TCAAAGCACC 
TCTGTCAAGA GCCCAAAAAC TGTGTCCCCA CCTATCAGGA AAGGCTGGAG CATGTCAGAG 
CAGAGTGAAG AGTCTGTGGG TGGAAGAGTT GCAGAAAGGA AACAAGTGGA AAATGCCAAG 
GCTTCTAAGA AGAATGGGAA TGTGGGAAAA ACAACCTGGC AAAACAAAGA ATCTAAAGGA 
GAGACAGGGA AGAGAAGTAA GGAAGGTCAT AGTTTGGAGA TGGAGAATGA GAATCTTGTA 
GAAAATGGTG CAGACTCCGA TGAAGATGAT AACAGCTTCC TCAAACAACA ATCTCCACAA 
GAACCCAAGT CTCTGAATTG GTCGAGTTTT GTAGACAACA CCTTTGCTGA AGAATTCACT 
ACTCAGAATC AGAAATCCCA GGATGTGGAA CTCTGGGAGG GAGAAGTGGT CAAAGAGCTC 
TCTGTGGAAG AACAGATAAA GAGAAATCGG TATTATGATG AGGATGAGGA TGAAGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMA1 ( NM_016357.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_057441.1 Homo sapiens LIM domain and actin binding 1 (LIMA1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu02217
Clone ID Related Accession (Same CDS sequence)	NM_016357.4 , NM_016357.5
Accession Version	NM_016357.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2280bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Homo sapiens(human)
Product	LIM domain and actin-binding protein 1 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP281423.1, BC110815.1 and AL136911.1. On Jun 2, 2019 this sequence version replaced NM_016357.4. Transcript Variant: This variant (2) uses an alternate in-frame acceptor splice site at an internal coding exon compared to variant 1. This results in an isoform (2, also known as EPLIN-beta) that is 1 aa shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC110815.1, AL136911.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGAATCAT CTCCATTTAA TAGACGGCAA TGGACCTCAC TATCATTGAG GGTAACAGCC 
AAAGAACTTT CTCTTGTCAA CAAGAACAAG TCATCGGCTA TTGTGGAAAT ATTCTCCAAG 
TACCAGAAAG CAGCTGAAGA AACAAACATG GAGAAGAAGA GAAGTAACAC CGAAAATCTC 
TCCCAGCACT TTAGAAAGGG GACCCTGACT GTGTTAAAGA AGAAGTGGGA GAACCCAGGG 
CTGGGAGCAG AGTCTCACAC AGACTCTCTA CGGAACAGCA GCACTGAGAT TAGGCACAGA 
GCAGACCATC CTCCTGCTGA AGTGACAAGC CACGCTGCTT CTGGAGCCAA AGCTGACCAA 
GAAGAACAAA TCCACCCCAG ATCTAGACTC AGGTCACCTC CTGAAGCCCT CGTTCAGGGT 
CGATATCCCC ACATCAAGGA CGGTGAGGAT CTTAAAGACC ACTCAACAGA AAGTAAAAAA 
ATGGAAAATT GTCTAGGAGA ATCCAGGCAT GAAGTAGAAA AATCAGAAAT CAGTGAAAAC 
ACAGATGCTT CGGGCAAAAT AGAGAAATAT AATGTTCCGC TGAACAGGCT TAAGATGATG 
TTTGAGAAAG GTGAACCAAC TCAAACTAAG ATTCTCCGGG CCCAAAGCCG AAGTGCAAGT 
GGAAGGAAGA TCTCTGAAAA CAGCTATTCT CTAGATGACC TGGAAATAGG CCCAGGTCAG 
TTGTCATCTT CTACATTTGA CTCGGAGAAA AATGAGAGTA GACGAAATCT GGAACTTCCA 
CGCCTCTCAG AAACCTCTAT AAAGGATCGA ATGGCCAAGT ACCAGGCAGC TGTGTCCAAA 
CAAAGCAGCT CAACCAACTA TACAAATGAG CTGAAAGCCA GTGGTGGCGA AATCAAAATT 
CATAAAATGG AGCAAAAGGA GAATGTGCCC CCAGGTCCTG AGGTCTGCAT CACCCATCAG 
GAAGGGGAAA AGATTTCTGC AAATGAGAAT AGCCTGGCAG TCCGTTCCAC CCCTGCCGAA 
GATGACTCCC GTGACTCCCA GGTTAAGAGT GAGGTTCAAC AGCCTGTCCA TCCCAAGCCA 
CTAAGTCCAG ATTCCAGAGC CTCCAGTCTT TCTGAAAGTT CTCCTCCCAA AGCAATGAAG 
AAGTTTCAGG CACCTGCAAG AGAGACCTGC GTGGAATGTC AGAAGACAGT CTATCCAATG 
GAGCGTCTCT TGGCCAACCA GCAGGTGTTT CACATCAGCT GCTTCCGTTG CTCCTATTGC 
AACAACAAAC TCAGTCTAGG AACATATGCA TCTTTACATG GAAGAATCTA TTGTAAGCCT 
CACTTCAATC AACTCTTTAA ATCTAAGGGC AACTATGATG AAGGCTTTGG GCACAGACCA 
CACAAGGATC TATGGGCAAG CAAAAATGAA AACGAAGAGA TTTTGGAGAG ACCAGCCCAG 
CTTGCAAATG CAAGGGAGAC CCCTCACAGC CCAGGGGTAG AAGATGCCCC TATTGCTAAG 
GTGGGTGTCC TGGCTGCAAG TATGGAAGCC AAGGCCTCCT CTCAGCAGGA GAAGGAAGAC 
AAGCCAGCTG AAACCAAGAA GCTGAGGATC GCCTGGCCAC CCCCCACTGA ACTTGGAAGT 
TCAGGAAGTG CCTTGGAGGA AGGGATCAAA ATGTCAAAGC CCAAATGGCC TCCTGAAGAC 
GAAATCAGCA AGCCCGAAGT TCCTGAGGAT GTCGATCTAG ATCTGAAGAA GCTAAGACGA 
TCTTCTTCAC TGAAGGAAAG AAGCCGCCCA TTCACTGTAG CAGCTTCATT TCAAAGCACC 
TCTGTCAAGA GCCCAAAAAC TGTGTCCCCA CCTATCAGGA AAGGCTGGAG CATGTCAGAG 
CAGAGTGAAG AGTCTGTGGG TGGAAGAGTT GCAGAAAGGA AACAAGTGGA AAATGCCAAG 
GCTTCTAAGA AGAATGGGAA TGTGGGAAAA ACAACCTGGC AAAACAAAGA ATCTAAAGGA 
GAGACAGGGA AGAGAAGTAA GGAAGGTCAT AGTTTGGAGA TGGAGAATGA GAATCTTGTA 
GAAAATGGTG CAGACTCCGA TGAAGATGAT AACAGCTTCC TCAAACAACA ATCTCCACAA 
GAACCCAAGT CTCTGAATTG GTCGAGTTTT GTAGACAACA CCTTTGCTGA AGAATTCACT 
ACTCAGAATC AGAAATCCCA GGATGTGGAA CTCTGGGAGG GAGAAGTGGT CAAAGAGCTC 
TCTGTGGAAG AACAGATAAA GAGAAATCGG TATTATGATG AGGATGAGGA TGAAGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_057441.1
CDS	125..2404
Translation	MESSPFNRRQWTSLSLRVTAKELSLVNKNKSSAIVEIFSKYQKAAEETNMEKKRSNTENLSQHFRKGTLTVLKKKWENPGLGAESHTDSLRNSSTEIRHRADHPPAEVTSHAASGAKADQEEQIHPRSRLRSPPEALVQGRYPHIKDGEDLKDHSTESKKMENCLGESRHEVEKSEISENTDASGKIEKYNVPLNRLKMMFEKGEPTQTKILRAQSRSASGRKISENSYSLDDLEIGPGQLSSSTFDSEKNESRRNLELPRLSETSIKDRMAKYQAAVSKQSSSTNYTNELKASGGEIKIHKMEQKENVPPGPEVCITHQEGEKISANENSLAVRSTPAEDDSRDSQVKSEVQQPVHPKPLSPDSRASSLSESSPPKAMKKFQAPARETCVECQKTVYPMERLLANQQVFHISCFRCSYCNNKLSLGTYASLHGRIYCKPHFNQLFKSKGNYDEGFGHRPHKDLWASKNENEEILERPAQLANARETPHSPGVEDAPIAKVGVLAASMEAKASSQQEKEDKPAETKKLRIAWPPPTELGSSGSALEEGIKMSKPKWPPEDEISKPEVPEDVDLDLKKLRRSSSLKERSRPFTVAASFQSTSVKSPKTVSPPIRKGWSMSEQSEESVGGRVAERKQVENAKASKKNGNVGKTTWQNKESKGETGKRSKEGHSLEMENENLVENGADSDEDDNSFLKQQSPQEPKSLNWSSFVDNTFAEEFTTQNQKSQDVELWEGEVVKELSVEEQIKRNRYYDEDEDEE

Target ORF information:

RefSeq Version	NM_016357.5
Organism	Homo sapiens(human)
Definition	Homo sapiens LIM domain and actin binding 1 (LIMA1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_016357.5

ORF Insert Sequence:

ATGGAATCAT CTCCATTTAA TAGACGGCAA TGGACCTCAC TATCATTGAG GGTAACAGCC 
AAAGAACTTT CTCTTGTCAA CAAGAACAAG TCATCGGCTA TTGTGGAAAT ATTCTCCAAG 
TACCAGAAAG CAGCTGAAGA AACAAACATG GAGAAGAAGA GAAGTAACAC CGAAAATCTC 
TCCCAGCACT TTAGAAAGGG GACCCTGACT GTGTTAAAGA AGAAGTGGGA GAACCCAGGG 
CTGGGAGCAG AGTCTCACAC AGACTCTCTA CGGAACAGCA GCACTGAGAT TAGGCACAGA 
GCAGACCATC CTCCTGCTGA AGTGACAAGC CACGCTGCTT CTGGAGCCAA AGCTGACCAA 
GAAGAACAAA TCCACCCCAG ATCTAGACTC AGGTCACCTC CTGAAGCCCT CGTTCAGGGT 
CGATATCCCC ACATCAAGGA CGGTGAGGAT CTTAAAGACC ACTCAACAGA AAGTAAAAAA 
ATGGAAAATT GTCTAGGAGA ATCCAGGCAT GAAGTAGAAA AATCAGAAAT CAGTGAAAAC 
ACAGATGCTT CGGGCAAAAT AGAGAAATAT AATGTTCCGC TGAACAGGCT TAAGATGATG 
TTTGAGAAAG GTGAACCAAC TCAAACTAAG ATTCTCCGGG CCCAAAGCCG AAGTGCAAGT 
GGAAGGAAGA TCTCTGAAAA CAGCTATTCT CTAGATGACC TGGAAATAGG CCCAGGTCAG 
TTGTCATCTT CTACATTTGA CTCGGAGAAA AATGAGAGTA GACGAAATCT GGAACTTCCA 
CGCCTCTCAG AAACCTCTAT AAAGGATCGA ATGGCCAAGT ACCAGGCAGC TGTGTCCAAA 
CAAAGCAGCT CAACCAACTA TACAAATGAG CTGAAAGCCA GTGGTGGCGA AATCAAAATT 
CATAAAATGG AGCAAAAGGA GAATGTGCCC CCAGGTCCTG AGGTCTGCAT CACCCATCAG 
GAAGGGGAAA AGATTTCTGC AAATGAGAAT AGCCTGGCAG TCCGTTCCAC CCCTGCCGAA 
GATGACTCCC GTGACTCCCA GGTTAAGAGT GAGGTTCAAC AGCCTGTCCA TCCCAAGCCA 
CTAAGTCCAG ATTCCAGAGC CTCCAGTCTT TCTGAAAGTT CTCCTCCCAA AGCAATGAAG 
AAGTTTCAGG CACCTGCAAG AGAGACCTGC GTGGAATGTC AGAAGACAGT CTATCCAATG 
GAGCGTCTCT TGGCCAACCA GCAGGTGTTT CACATCAGCT GCTTCCGTTG CTCCTATTGC 
AACAACAAAC TCAGTCTAGG AACATATGCA TCTTTACATG GAAGAATCTA TTGTAAGCCT 
CACTTCAATC AACTCTTTAA ATCTAAGGGC AACTATGATG AAGGCTTTGG GCACAGACCA 
CACAAGGATC TATGGGCAAG CAAAAATGAA AACGAAGAGA TTTTGGAGAG ACCAGCCCAG 
CTTGCAAATG CAAGGGAGAC CCCTCACAGC CCAGGGGTAG AAGATGCCCC TATTGCTAAG 
GTGGGTGTCC TGGCTGCAAG TATGGAAGCC AAGGCCTCCT CTCAGCAGGA GAAGGAAGAC 
AAGCCAGCTG AAACCAAGAA GCTGAGGATC GCCTGGCCAC CCCCCACTGA ACTTGGAAGT 
TCAGGAAGTG CCTTGGAGGA AGGGATCAAA ATGTCAAAGC CCAAATGGCC TCCTGAAGAC 
GAAATCAGCA AGCCCGAAGT TCCTGAGGAT GTCGATCTAG ATCTGAAGAA GCTAAGACGA 
TCTTCTTCAC TGAAGGAAAG AAGCCGCCCA TTCACTGTAG CAGCTTCATT TCAAAGCACC 
TCTGTCAAGA GCCCAAAAAC TGTGTCCCCA CCTATCAGGA AAGGCTGGAG CATGTCAGAG 
CAGAGTGAAG AGTCTGTGGG TGGAAGAGTT GCAGAAAGGA AACAAGTGGA AAATGCCAAG 
GCTTCTAAGA AGAATGGGAA TGTGGGAAAA ACAACCTGGC AAAACAAAGA ATCTAAAGGA 
GAGACAGGGA AGAGAAGTAA GGAAGGTCAT AGTTTGGAGA TGGAGAATGA GAATCTTGTA 
GAAAATGGTG CAGACTCCGA TGAAGATGAT AACAGCTTCC TCAAACAACA ATCTCCACAA 
GAACCCAAGT CTCTGAATTG GTCGAGTTTT GTAGACAACA CCTTTGCTGA AGAATTCACT 
ACTCAGAATC AGAAATCCCA GGATGTGGAA CTCTGGGAGG GAGAAGTGGT CAAAGAGCTC 
TCTGTGGAAG AACAGATAAA GAGAAATCGG TATTATGATG AGGATGAGGA TGAAGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMA1 ( NM_001243775.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001230704.1 Homo sapiens LIM domain and actin binding 1 (LIMA1), transcript variant 4, mRNA.

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CloneID	OHu02323
Clone ID Related Accession (Same CDS sequence)	NM_001243775.1 , XM_017019430.1
Accession Version	NM_001243775.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1374bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-04
Organism	Homo sapiens(human)
Product	LIM domain and actin-binding protein 1 isoform 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF157325.1, AK023649.1, BC110815.1, AL136911.1 and AA772616.1. Transcript Variant: This variant (4) differs at the 5' end, and uses an alternate in-frame acceptor splice site at an internal coding exon compared to variant 1. This results in translation initiation from an in-frame downstream AUG, and an isoform (4) with a shorter N-terminus and lacking an internal aa compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF157325.1, SRR1803612.183859.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2152798, SAMEA2467144 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGCAAA AGGAGAATGT GCCCCCAGGT CCTGAGGTCT GCATCACCCA TCAGGAAGGG 
GAAAAGATTT CTGCAAATGA GAATAGCCTG GCAGTCCGTT CCACCCCTGC CGAAGATGAC 
TCCCGTGACT CCCAGGTTAA GAGTGAGGTT CAACAGCCTG TCCATCCCAA GCCACTAAGT 
CCAGATTCCA GAGCCTCCAG TCTTTCTGAA AGTTCTCCTC CCAAAGCAAT GAAGAAGTTT 
CAGGCACCTG CAAGAGAGAC CTGCGTGGAA TGTCAGAAGA CAGTCTATCC AATGGAGCGT 
CTCTTGGCCA ACCAGCAGGT GTTTCACATC AGCTGCTTCC GTTGCTCCTA TTGCAACAAC 
AAACTCAGTC TAGGAACATA TGCATCTTTA CATGGAAGAA TCTATTGTAA GCCTCACTTC 
AATCAACTCT TTAAATCTAA GGGCAACTAT GATGAAGGCT TTGGGCACAG ACCACACAAG 
GATCTATGGG CAAGCAAAAA TGAAAACGAA GAGATTTTGG AGAGACCAGC CCAGCTTGCA 
AATGCAAGGG AGACCCCTCA CAGCCCAGGG GTAGAAGATG CCCCTATTGC TAAGGTGGGT 
GTCCTGGCTG CAAGTATGGA AGCCAAGGCC TCCTCTCAGC AGGAGAAGGA AGACAAGCCA 
GCTGAAACCA AGAAGCTGAG GATCGCCTGG CCACCCCCCA CTGAACTTGG AAGTTCAGGA 
AGTGCCTTGG AGGAAGGGAT CAAAATGTCA AAGCCCAAAT GGCCTCCTGA AGACGAAATC 
AGCAAGCCCG AAGTTCCTGA GGATGTCGAT CTAGATCTGA AGAAGCTAAG ACGATCTTCT 
TCACTGAAGG AAAGAAGCCG CCCATTCACT GTAGCAGCTT CATTTCAAAG CACCTCTGTC 
AAGAGCCCAA AAACTGTGTC CCCACCTATC AGGAAAGGCT GGAGCATGTC AGAGCAGAGT 
GAAGAGTCTG TGGGTGGAAG AGTTGCAGAA AGGAAACAAG TGGAAAATGC CAAGGCTTCT 
AAGAAGAATG GGAATGTGGG AAAAACAACC TGGCAAAACA AAGAATCTAA AGGAGAGACA 
GGGAAGAGAA GTAAGGAAGG TCATAGTTTG GAGATGGAGA ATGAGAATCT TGTAGAAAAT 
GGTGCAGACT CCGATGAAGA TGATAACAGC TTCCTCAAAC AACAATCTCC ACAAGAACCC 
AAGTCTCTGA ATTGGTCGAG TTTTGTAGAC AACACCTTTG CTGAAGAATT CACTACTCAG 
AATCAGAAAT CCCAGGATGT GGAACTCTGG GAGGGAGAAG TGGTCAAAGA GCTCTCTGTG 
GAAGAACAGA TAAAGAGAAA TCGGTATTAT GATGAGGATG AGGATGAAGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001230704.1
CDS	692..2065
Translation	MEQKENVPPGPEVCITHQEGEKISANENSLAVRSTPAEDDSRDSQVKSEVQQPVHPKPLSPDSRASSLSESSPPKAMKKFQAPARETCVECQKTVYPMERLLANQQVFHISCFRCSYCNNKLSLGTYASLHGRIYCKPHFNQLFKSKGNYDEGFGHRPHKDLWASKNENEEILERPAQLANARETPHSPGVEDAPIAKVGVLAASMEAKASSQQEKEDKPAETKKLRIAWPPPTELGSSGSALEEGIKMSKPKWPPEDEISKPEVPEDVDLDLKKLRRSSSLKERSRPFTVAASFQSTSVKSPKTVSPPIRKGWSMSEQSEESVGGRVAERKQVENAKASKKNGNVGKTTWQNKESKGETGKRSKEGHSLEMENENLVENGADSDEDDNSFLKQQSPQEPKSLNWSSFVDNTFAEEFTTQNQKSQDVELWEGEVVKELSVEEQIKRNRYYDEDEDEE

Target ORF information:

RefSeq Version	NM_001243775.1
Organism	Homo sapiens(human)
Definition	Homo sapiens LIM domain and actin binding 1 (LIMA1), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001243775.1

ORF Insert Sequence:

ATGGAGCAAA AGGAGAATGT GCCCCCAGGT CCTGAGGTCT GCATCACCCA TCAGGAAGGG 
GAAAAGATTT CTGCAAATGA GAATAGCCTG GCAGTCCGTT CCACCCCTGC CGAAGATGAC 
TCCCGTGACT CCCAGGTTAA GAGTGAGGTT CAACAGCCTG TCCATCCCAA GCCACTAAGT 
CCAGATTCCA GAGCCTCCAG TCTTTCTGAA AGTTCTCCTC CCAAAGCAAT GAAGAAGTTT 
CAGGCACCTG CAAGAGAGAC CTGCGTGGAA TGTCAGAAGA CAGTCTATCC AATGGAGCGT 
CTCTTGGCCA ACCAGCAGGT GTTTCACATC AGCTGCTTCC GTTGCTCCTA TTGCAACAAC 
AAACTCAGTC TAGGAACATA TGCATCTTTA CATGGAAGAA TCTATTGTAA GCCTCACTTC 
AATCAACTCT TTAAATCTAA GGGCAACTAT GATGAAGGCT TTGGGCACAG ACCACACAAG 
GATCTATGGG CAAGCAAAAA TGAAAACGAA GAGATTTTGG AGAGACCAGC CCAGCTTGCA 
AATGCAAGGG AGACCCCTCA CAGCCCAGGG GTAGAAGATG CCCCTATTGC TAAGGTGGGT 
GTCCTGGCTG CAAGTATGGA AGCCAAGGCC TCCTCTCAGC AGGAGAAGGA AGACAAGCCA 
GCTGAAACCA AGAAGCTGAG GATCGCCTGG CCACCCCCCA CTGAACTTGG AAGTTCAGGA 
AGTGCCTTGG AGGAAGGGAT CAAAATGTCA AAGCCCAAAT GGCCTCCTGA AGACGAAATC 
AGCAAGCCCG AAGTTCCTGA GGATGTCGAT CTAGATCTGA AGAAGCTAAG ACGATCTTCT 
TCACTGAAGG AAAGAAGCCG CCCATTCACT GTAGCAGCTT CATTTCAAAG CACCTCTGTC 
AAGAGCCCAA AAACTGTGTC CCCACCTATC AGGAAAGGCT GGAGCATGTC AGAGCAGAGT 
GAAGAGTCTG TGGGTGGAAG AGTTGCAGAA AGGAAACAAG TGGAAAATGC CAAGGCTTCT 
AAGAAGAATG GGAATGTGGG AAAAACAACC TGGCAAAACA AAGAATCTAA AGGAGAGACA 
GGGAAGAGAA GTAAGGAAGG TCATAGTTTG GAGATGGAGA ATGAGAATCT TGTAGAAAAT 
GGTGCAGACT CCGATGAAGA TGATAACAGC TTCCTCAAAC AACAATCTCC ACAAGAACCC 
AAGTCTCTGA ATTGGTCGAG TTTTGTAGAC AACACCTTTG CTGAAGAATT CACTACTCAG 
AATCAGAAAT CCCAGGATGT GGAACTCTGG GAGGGAGAAG TGGTCAAAGA GCTCTCTGTG 
GAAGAACAGA TAAAGAGAAA TCGGTATTAT GATGAGGATG AGGATGAAGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMA1 ( NM_001113546.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001107018.1 Homo sapiens LIM domain and actin binding 1 (LIMA1), transcript variant 1, mRNA.

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CloneID	OHu18730
Clone ID Related Accession (Same CDS sequence)	NM_001113546.2 , XM_011538455.2 , NM_001113546.1
Accession Version	NM_001113546.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2283bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-21
Organism	Homo sapiens(human)
Product	LIM domain and actin-binding protein 1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP281423.1, AB209512.1, BC110815.1 and AL136911.1. This sequence is a reference standard in the RefSeqGene project. On Jun 2, 2019 this sequence version replaced NM_001113546.1. Transcript Variant: This variant (1) encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR7346977.1666121.1, SRR7346977.2103736.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAATCAT CTCCATTTAA TAGACGGCAA TGGACCTCAC TATCATTGAG GGTAACAGCC 
AAAGAACTTT CTCTTGTCAA CAAGAACAAG TCATCGGCTA TTGTGGAAAT ATTCTCCAAG 
TACCAGAAAG CAGCTGAAGA AACAAACATG GAGAAGAAGA GAAGTAACAC CGAAAATCTC 
TCCCAGCACT TTAGAAAGGG GACCCTGACT GTGTTAAAGA AGAAGTGGGA GAACCCAGGG 
CTGGGAGCAG AGTCTCACAC AGACTCTCTA CGGAACAGCA GCACTGAGAT TAGGCACAGA 
GCAGACCATC CTCCTGCTGA AGTGACAAGC CACGCTGCTT CTGGAGCCAA AGCTGACCAA 
GAAGAACAAA TCCACCCCAG ATCTAGACTC AGGTCACCTC CTGAAGCCCT CGTTCAGGGT 
CGATATCCCC ACATCAAGGA CGGTGAGGAT CTTAAAGACC ACTCAACAGA AAGTAAAAAA 
ATGGAAAATT GTCTAGGAGA ATCCAGGCAT GAAGTAGAAA AATCAGAAAT CAGTGAAAAC 
ACAGATGCTT CGGGCAAAAT AGAGAAATAT AATGTTCCGC TGAACAGGCT TAAGATGATG 
TTTGAGAAAG GTGAACCAAC TCAAACTAAG ATTCTCCGGG CCCAAAGCCG AAGTGCAAGT 
GGAAGGAAGA TCTCTGAAAA CAGCTATTCT CTAGATGACC TGGAAATAGG CCCAGGTCAG 
TTGTCATCTT CTACATTTGA CTCGGAGAAA AATGAGAGTA GACGAAATCT GGAACTTCCA 
CGCCTCTCAG AAACCTCTAT AAAGGATCGA ATGGCCAAGT ACCAGGCAGC TGTGTCCAAA 
CAAAGCAGCT CAACCAACTA TACAAATGAG CTGAAAGCCA GTGGTGGCGA AATCAAAATT 
CATAAAATGG AGCAAAAGGA GAATGTGCCC CCAGGTCCTG AGGTCTGCAT CACCCATCAG 
GAAGGGGAAA AGATTTCTGC AAATGAGAAT AGCCTGGCAG TCCGTTCCAC CCCTGCCGAA 
GATGACTCCC CAGGTGACTC CCAGGTTAAG AGTGAGGTTC AACAGCCTGT CCATCCCAAG 
CCACTAAGTC CAGATTCCAG AGCCTCCAGT CTTTCTGAAA GTTCTCCTCC CAAAGCAATG 
AAGAAGTTTC AGGCACCTGC AAGAGAGACC TGCGTGGAAT GTCAGAAGAC AGTCTATCCA 
ATGGAGCGTC TCTTGGCCAA CCAGCAGGTG TTTCACATCA GCTGCTTCCG TTGCTCCTAT 
TGCAACAACA AACTCAGTCT AGGAACATAT GCATCTTTAC ATGGAAGAAT CTATTGTAAG 
CCTCACTTCA ATCAACTCTT TAAATCTAAG GGCAACTATG ATGAAGGCTT TGGGCACAGA 
CCACACAAGG ATCTATGGGC AAGCAAAAAT GAAAACGAAG AGATTTTGGA GAGACCAGCC 
CAGCTTGCAA ATGCAAGGGA GACCCCTCAC AGCCCAGGGG TAGAAGATGC CCCTATTGCT 
AAGGTGGGTG TCCTGGCTGC AAGTATGGAA GCCAAGGCCT CCTCTCAGCA GGAGAAGGAA 
GACAAGCCAG CTGAAACCAA GAAGCTGAGG ATCGCCTGGC CACCCCCCAC TGAACTTGGA 
AGTTCAGGAA GTGCCTTGGA GGAAGGGATC AAAATGTCAA AGCCCAAATG GCCTCCTGAA 
GACGAAATCA GCAAGCCCGA AGTTCCTGAG GATGTCGATC TAGATCTGAA GAAGCTAAGA 
CGATCTTCTT CACTGAAGGA AAGAAGCCGC CCATTCACTG TAGCAGCTTC ATTTCAAAGC 
ACCTCTGTCA AGAGCCCAAA AACTGTGTCC CCACCTATCA GGAAAGGCTG GAGCATGTCA 
GAGCAGAGTG AAGAGTCTGT GGGTGGAAGA GTTGCAGAAA GGAAACAAGT GGAAAATGCC 
AAGGCTTCTA AGAAGAATGG GAATGTGGGA AAAACAACCT GGCAAAACAA AGAATCTAAA 
GGAGAGACAG GGAAGAGAAG TAAGGAAGGT CATAGTTTGG AGATGGAGAA TGAGAATCTT 
GTAGAAAATG GTGCAGACTC CGATGAAGAT GATAACAGCT TCCTCAAACA ACAATCTCCA 
CAAGAACCCA AGTCTCTGAA TTGGTCGAGT TTTGTAGACA ACACCTTTGC TGAAGAATTC 
ACTACTCAGA ATCAGAAATC CCAGGATGTG GAACTCTGGG AGGGAGAAGT GGTCAAAGAG 
CTCTCTGTGG AAGAACAGAT AAAGAGAAAT CGGTATTATG ATGAGGATGA GGATGAAGAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001107018.1
CDS	125..2407
Translation	MESSPFNRRQWTSLSLRVTAKELSLVNKNKSSAIVEIFSKYQKAAEETNMEKKRSNTENLSQHFRKGTLTVLKKKWENPGLGAESHTDSLRNSSTEIRHRADHPPAEVTSHAASGAKADQEEQIHPRSRLRSPPEALVQGRYPHIKDGEDLKDHSTESKKMENCLGESRHEVEKSEISENTDASGKIEKYNVPLNRLKMMFEKGEPTQTKILRAQSRSASGRKISENSYSLDDLEIGPGQLSSSTFDSEKNESRRNLELPRLSETSIKDRMAKYQAAVSKQSSSTNYTNELKASGGEIKIHKMEQKENVPPGPEVCITHQEGEKISANENSLAVRSTPAEDDSPGDSQVKSEVQQPVHPKPLSPDSRASSLSESSPPKAMKKFQAPARETCVECQKTVYPMERLLANQQVFHISCFRCSYCNNKLSLGTYASLHGRIYCKPHFNQLFKSKGNYDEGFGHRPHKDLWASKNENEEILERPAQLANARETPHSPGVEDAPIAKVGVLAASMEAKASSQQEKEDKPAETKKLRIAWPPPTELGSSGSALEEGIKMSKPKWPPEDEISKPEVPEDVDLDLKKLRRSSSLKERSRPFTVAASFQSTSVKSPKTVSPPIRKGWSMSEQSEESVGGRVAERKQVENAKASKKNGNVGKTTWQNKESKGETGKRSKEGHSLEMENENLVENGADSDEDDNSFLKQQSPQEPKSLNWSSFVDNTFAEEFTTQNQKSQDVELWEGEVVKELSVEEQIKRNRYYDEDEDEE

Target ORF information:

RefSeq Version	NM_001113546.2
Organism	Homo sapiens(human)
Definition	Homo sapiens LIM domain and actin binding 1 (LIMA1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001113546.2

ORF Insert Sequence:

ATGGAATCAT CTCCATTTAA TAGACGGCAA TGGACCTCAC TATCATTGAG GGTAACAGCC 
AAAGAACTTT CTCTTGTCAA CAAGAACAAG TCATCGGCTA TTGTGGAAAT ATTCTCCAAG 
TACCAGAAAG CAGCTGAAGA AACAAACATG GAGAAGAAGA GAAGTAACAC CGAAAATCTC 
TCCCAGCACT TTAGAAAGGG GACCCTGACT GTGTTAAAGA AGAAGTGGGA GAACCCAGGG 
CTGGGAGCAG AGTCTCACAC AGACTCTCTA CGGAACAGCA GCACTGAGAT TAGGCACAGA 
GCAGACCATC CTCCTGCTGA AGTGACAAGC CACGCTGCTT CTGGAGCCAA AGCTGACCAA 
GAAGAACAAA TCCACCCCAG ATCTAGACTC AGGTCACCTC CTGAAGCCCT CGTTCAGGGT 
CGATATCCCC ACATCAAGGA CGGTGAGGAT CTTAAAGACC ACTCAACAGA AAGTAAAAAA 
ATGGAAAATT GTCTAGGAGA ATCCAGGCAT GAAGTAGAAA AATCAGAAAT CAGTGAAAAC 
ACAGATGCTT CGGGCAAAAT AGAGAAATAT AATGTTCCGC TGAACAGGCT TAAGATGATG 
TTTGAGAAAG GTGAACCAAC TCAAACTAAG ATTCTCCGGG CCCAAAGCCG AAGTGCAAGT 
GGAAGGAAGA TCTCTGAAAA CAGCTATTCT CTAGATGACC TGGAAATAGG CCCAGGTCAG 
TTGTCATCTT CTACATTTGA CTCGGAGAAA AATGAGAGTA GACGAAATCT GGAACTTCCA 
CGCCTCTCAG AAACCTCTAT AAAGGATCGA ATGGCCAAGT ACCAGGCAGC TGTGTCCAAA 
CAAAGCAGCT CAACCAACTA TACAAATGAG CTGAAAGCCA GTGGTGGCGA AATCAAAATT 
CATAAAATGG AGCAAAAGGA GAATGTGCCC CCAGGTCCTG AGGTCTGCAT CACCCATCAG 
GAAGGGGAAA AGATTTCTGC AAATGAGAAT AGCCTGGCAG TCCGTTCCAC CCCTGCCGAA 
GATGACTCCC CAGGTGACTC CCAGGTTAAG AGTGAGGTTC AACAGCCTGT CCATCCCAAG 
CCACTAAGTC CAGATTCCAG AGCCTCCAGT CTTTCTGAAA GTTCTCCTCC CAAAGCAATG 
AAGAAGTTTC AGGCACCTGC AAGAGAGACC TGCGTGGAAT GTCAGAAGAC AGTCTATCCA 
ATGGAGCGTC TCTTGGCCAA CCAGCAGGTG TTTCACATCA GCTGCTTCCG TTGCTCCTAT 
TGCAACAACA AACTCAGTCT AGGAACATAT GCATCTTTAC ATGGAAGAAT CTATTGTAAG 
CCTCACTTCA ATCAACTCTT TAAATCTAAG GGCAACTATG ATGAAGGCTT TGGGCACAGA 
CCACACAAGG ATCTATGGGC AAGCAAAAAT GAAAACGAAG AGATTTTGGA GAGACCAGCC 
CAGCTTGCAA ATGCAAGGGA GACCCCTCAC AGCCCAGGGG TAGAAGATGC CCCTATTGCT 
AAGGTGGGTG TCCTGGCTGC AAGTATGGAA GCCAAGGCCT CCTCTCAGCA GGAGAAGGAA 
GACAAGCCAG CTGAAACCAA GAAGCTGAGG ATCGCCTGGC CACCCCCCAC TGAACTTGGA 
AGTTCAGGAA GTGCCTTGGA GGAAGGGATC AAAATGTCAA AGCCCAAATG GCCTCCTGAA 
GACGAAATCA GCAAGCCCGA AGTTCCTGAG GATGTCGATC TAGATCTGAA GAAGCTAAGA 
CGATCTTCTT CACTGAAGGA AAGAAGCCGC CCATTCACTG TAGCAGCTTC ATTTCAAAGC 
ACCTCTGTCA AGAGCCCAAA AACTGTGTCC CCACCTATCA GGAAAGGCTG GAGCATGTCA 
GAGCAGAGTG AAGAGTCTGT GGGTGGAAGA GTTGCAGAAA GGAAACAAGT GGAAAATGCC 
AAGGCTTCTA AGAAGAATGG GAATGTGGGA AAAACAACCT GGCAAAACAA AGAATCTAAA 
GGAGAGACAG GGAAGAGAAG TAAGGAAGGT CATAGTTTGG AGATGGAGAA TGAGAATCTT 
GTAGAAAATG GTGCAGACTC CGATGAAGAT GATAACAGCT TCCTCAAACA ACAATCTCCA 
CAAGAACCCA AGTCTCTGAA TTGGTCGAGT TTTGTAGACA ACACCTTTGC TGAAGAATTC 
ACTACTCAGA ATCAGAAATC CCAGGATGTG GAACTCTGGG AGGGAGAAGT GGTCAAAGAG 
CTCTCTGTGG AAGAACAGAT AAAGAGAAAT CGGTATTATG ATGAGGATGA GGATGAAGAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMA1 ( NM_001113546.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001107018.1 Homo sapiens LIM domain and actin binding 1 (LIMA1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu18730
Clone ID Related Accession (Same CDS sequence)	NM_001113546.2 , XM_011538455.2 , NM_001113546.1
Accession Version	NM_001113546.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2283bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-03-11
Organism	Homo sapiens(human)
Product	LIM domain and actin-binding protein 1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP216703.1, BP281423.1, AB209512.1, BC110815.1, AL136911.1 and AA772616.1. This sequence is a reference standard in the RefSeqGene project. Transcript Variant: This variant (1) encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.216285.1, SRR1660803.240904.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAATCAT CTCCATTTAA TAGACGGCAA TGGACCTCAC TATCATTGAG GGTAACAGCC 
AAAGAACTTT CTCTTGTCAA CAAGAACAAG TCATCGGCTA TTGTGGAAAT ATTCTCCAAG 
TACCAGAAAG CAGCTGAAGA AACAAACATG GAGAAGAAGA GAAGTAACAC CGAAAATCTC 
TCCCAGCACT TTAGAAAGGG GACCCTGACT GTGTTAAAGA AGAAGTGGGA GAACCCAGGG 
CTGGGAGCAG AGTCTCACAC AGACTCTCTA CGGAACAGCA GCACTGAGAT TAGGCACAGA 
GCAGACCATC CTCCTGCTGA AGTGACAAGC CACGCTGCTT CTGGAGCCAA AGCTGACCAA 
GAAGAACAAA TCCACCCCAG ATCTAGACTC AGGTCACCTC CTGAAGCCCT CGTTCAGGGT 
CGATATCCCC ACATCAAGGA CGGTGAGGAT CTTAAAGACC ACTCAACAGA AAGTAAAAAA 
ATGGAAAATT GTCTAGGAGA ATCCAGGCAT GAAGTAGAAA AATCAGAAAT CAGTGAAAAC 
ACAGATGCTT CGGGCAAAAT AGAGAAATAT AATGTTCCGC TGAACAGGCT TAAGATGATG 
TTTGAGAAAG GTGAACCAAC TCAAACTAAG ATTCTCCGGG CCCAAAGCCG AAGTGCAAGT 
GGAAGGAAGA TCTCTGAAAA CAGCTATTCT CTAGATGACC TGGAAATAGG CCCAGGTCAG 
TTGTCATCTT CTACATTTGA CTCGGAGAAA AATGAGAGTA GACGAAATCT GGAACTTCCA 
CGCCTCTCAG AAACCTCTAT AAAGGATCGA ATGGCCAAGT ACCAGGCAGC TGTGTCCAAA 
CAAAGCAGCT CAACCAACTA TACAAATGAG CTGAAAGCCA GTGGTGGCGA AATCAAAATT 
CATAAAATGG AGCAAAAGGA GAATGTGCCC CCAGGTCCTG AGGTCTGCAT CACCCATCAG 
GAAGGGGAAA AGATTTCTGC AAATGAGAAT AGCCTGGCAG TCCGTTCCAC CCCTGCCGAA 
GATGACTCCC CAGGTGACTC CCAGGTTAAG AGTGAGGTTC AACAGCCTGT CCATCCCAAG 
CCACTAAGTC CAGATTCCAG AGCCTCCAGT CTTTCTGAAA GTTCTCCTCC CAAAGCAATG 
AAGAAGTTTC AGGCACCTGC AAGAGAGACC TGCGTGGAAT GTCAGAAGAC AGTCTATCCA 
ATGGAGCGTC TCTTGGCCAA CCAGCAGGTG TTTCACATCA GCTGCTTCCG TTGCTCCTAT 
TGCAACAACA AACTCAGTCT AGGAACATAT GCATCTTTAC ATGGAAGAAT CTATTGTAAG 
CCTCACTTCA ATCAACTCTT TAAATCTAAG GGCAACTATG ATGAAGGCTT TGGGCACAGA 
CCACACAAGG ATCTATGGGC AAGCAAAAAT GAAAACGAAG AGATTTTGGA GAGACCAGCC 
CAGCTTGCAA ATGCAAGGGA GACCCCTCAC AGCCCAGGGG TAGAAGATGC CCCTATTGCT 
AAGGTGGGTG TCCTGGCTGC AAGTATGGAA GCCAAGGCCT CCTCTCAGCA GGAGAAGGAA 
GACAAGCCAG CTGAAACCAA GAAGCTGAGG ATCGCCTGGC CACCCCCCAC TGAACTTGGA 
AGTTCAGGAA GTGCCTTGGA GGAAGGGATC AAAATGTCAA AGCCCAAATG GCCTCCTGAA 
GACGAAATCA GCAAGCCCGA AGTTCCTGAG GATGTCGATC TAGATCTGAA GAAGCTAAGA 
CGATCTTCTT CACTGAAGGA AAGAAGCCGC CCATTCACTG TAGCAGCTTC ATTTCAAAGC 
ACCTCTGTCA AGAGCCCAAA AACTGTGTCC CCACCTATCA GGAAAGGCTG GAGCATGTCA 
GAGCAGAGTG AAGAGTCTGT GGGTGGAAGA GTTGCAGAAA GGAAACAAGT GGAAAATGCC 
AAGGCTTCTA AGAAGAATGG GAATGTGGGA AAAACAACCT GGCAAAACAA AGAATCTAAA 
GGAGAGACAG GGAAGAGAAG TAAGGAAGGT CATAGTTTGG AGATGGAGAA TGAGAATCTT 
GTAGAAAATG GTGCAGACTC CGATGAAGAT GATAACAGCT TCCTCAAACA ACAATCTCCA 
CAAGAACCCA AGTCTCTGAA TTGGTCGAGT TTTGTAGACA ACACCTTTGC TGAAGAATTC 
ACTACTCAGA ATCAGAAATC CCAGGATGTG GAACTCTGGG AGGGAGAAGT GGTCAAAGAG 
CTCTCTGTGG AAGAACAGAT AAAGAGAAAT CGGTATTATG ATGAGGATGA GGATGAAGAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001107018.1
CDS	175..2457
Translation	MESSPFNRRQWTSLSLRVTAKELSLVNKNKSSAIVEIFSKYQKAAEETNMEKKRSNTENLSQHFRKGTLTVLKKKWENPGLGAESHTDSLRNSSTEIRHRADHPPAEVTSHAASGAKADQEEQIHPRSRLRSPPEALVQGRYPHIKDGEDLKDHSTESKKMENCLGESRHEVEKSEISENTDASGKIEKYNVPLNRLKMMFEKGEPTQTKILRAQSRSASGRKISENSYSLDDLEIGPGQLSSSTFDSEKNESRRNLELPRLSETSIKDRMAKYQAAVSKQSSSTNYTNELKASGGEIKIHKMEQKENVPPGPEVCITHQEGEKISANENSLAVRSTPAEDDSPGDSQVKSEVQQPVHPKPLSPDSRASSLSESSPPKAMKKFQAPARETCVECQKTVYPMERLLANQQVFHISCFRCSYCNNKLSLGTYASLHGRIYCKPHFNQLFKSKGNYDEGFGHRPHKDLWASKNENEEILERPAQLANARETPHSPGVEDAPIAKVGVLAASMEAKASSQQEKEDKPAETKKLRIAWPPPTELGSSGSALEEGIKMSKPKWPPEDEISKPEVPEDVDLDLKKLRRSSSLKERSRPFTVAASFQSTSVKSPKTVSPPIRKGWSMSEQSEESVGGRVAERKQVENAKASKKNGNVGKTTWQNKESKGETGKRSKEGHSLEMENENLVENGADSDEDDNSFLKQQSPQEPKSLNWSSFVDNTFAEEFTTQNQKSQDVELWEGEVVKELSVEEQIKRNRYYDEDEDEE

Target ORF information:

RefSeq Version	NM_001113546.1
Organism	Homo sapiens(human)
Definition	Homo sapiens LIM domain and actin binding 1 (LIMA1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001113546.1

ORF Insert Sequence:

ATGGAATCAT CTCCATTTAA TAGACGGCAA TGGACCTCAC TATCATTGAG GGTAACAGCC 
AAAGAACTTT CTCTTGTCAA CAAGAACAAG TCATCGGCTA TTGTGGAAAT ATTCTCCAAG 
TACCAGAAAG CAGCTGAAGA AACAAACATG GAGAAGAAGA GAAGTAACAC CGAAAATCTC 
TCCCAGCACT TTAGAAAGGG GACCCTGACT GTGTTAAAGA AGAAGTGGGA GAACCCAGGG 
CTGGGAGCAG AGTCTCACAC AGACTCTCTA CGGAACAGCA GCACTGAGAT TAGGCACAGA 
GCAGACCATC CTCCTGCTGA AGTGACAAGC CACGCTGCTT CTGGAGCCAA AGCTGACCAA 
GAAGAACAAA TCCACCCCAG ATCTAGACTC AGGTCACCTC CTGAAGCCCT CGTTCAGGGT 
CGATATCCCC ACATCAAGGA CGGTGAGGAT CTTAAAGACC ACTCAACAGA AAGTAAAAAA 
ATGGAAAATT GTCTAGGAGA ATCCAGGCAT GAAGTAGAAA AATCAGAAAT CAGTGAAAAC 
ACAGATGCTT CGGGCAAAAT AGAGAAATAT AATGTTCCGC TGAACAGGCT TAAGATGATG 
TTTGAGAAAG GTGAACCAAC TCAAACTAAG ATTCTCCGGG CCCAAAGCCG AAGTGCAAGT 
GGAAGGAAGA TCTCTGAAAA CAGCTATTCT CTAGATGACC TGGAAATAGG CCCAGGTCAG 
TTGTCATCTT CTACATTTGA CTCGGAGAAA AATGAGAGTA GACGAAATCT GGAACTTCCA 
CGCCTCTCAG AAACCTCTAT AAAGGATCGA ATGGCCAAGT ACCAGGCAGC TGTGTCCAAA 
CAAAGCAGCT CAACCAACTA TACAAATGAG CTGAAAGCCA GTGGTGGCGA AATCAAAATT 
CATAAAATGG AGCAAAAGGA GAATGTGCCC CCAGGTCCTG AGGTCTGCAT CACCCATCAG 
GAAGGGGAAA AGATTTCTGC AAATGAGAAT AGCCTGGCAG TCCGTTCCAC CCCTGCCGAA 
GATGACTCCC CAGGTGACTC CCAGGTTAAG AGTGAGGTTC AACAGCCTGT CCATCCCAAG 
CCACTAAGTC CAGATTCCAG AGCCTCCAGT CTTTCTGAAA GTTCTCCTCC CAAAGCAATG 
AAGAAGTTTC AGGCACCTGC AAGAGAGACC TGCGTGGAAT GTCAGAAGAC AGTCTATCCA 
ATGGAGCGTC TCTTGGCCAA CCAGCAGGTG TTTCACATCA GCTGCTTCCG TTGCTCCTAT 
TGCAACAACA AACTCAGTCT AGGAACATAT GCATCTTTAC ATGGAAGAAT CTATTGTAAG 
CCTCACTTCA ATCAACTCTT TAAATCTAAG GGCAACTATG ATGAAGGCTT TGGGCACAGA 
CCACACAAGG ATCTATGGGC AAGCAAAAAT GAAAACGAAG AGATTTTGGA GAGACCAGCC 
CAGCTTGCAA ATGCAAGGGA GACCCCTCAC AGCCCAGGGG TAGAAGATGC CCCTATTGCT 
AAGGTGGGTG TCCTGGCTGC AAGTATGGAA GCCAAGGCCT CCTCTCAGCA GGAGAAGGAA 
GACAAGCCAG CTGAAACCAA GAAGCTGAGG ATCGCCTGGC CACCCCCCAC TGAACTTGGA 
AGTTCAGGAA GTGCCTTGGA GGAAGGGATC AAAATGTCAA AGCCCAAATG GCCTCCTGAA 
GACGAAATCA GCAAGCCCGA AGTTCCTGAG GATGTCGATC TAGATCTGAA GAAGCTAAGA 
CGATCTTCTT CACTGAAGGA AAGAAGCCGC CCATTCACTG TAGCAGCTTC ATTTCAAAGC 
ACCTCTGTCA AGAGCCCAAA AACTGTGTCC CCACCTATCA GGAAAGGCTG GAGCATGTCA 
GAGCAGAGTG AAGAGTCTGT GGGTGGAAGA GTTGCAGAAA GGAAACAAGT GGAAAATGCC 
AAGGCTTCTA AGAAGAATGG GAATGTGGGA AAAACAACCT GGCAAAACAA AGAATCTAAA 
GGAGAGACAG GGAAGAGAAG TAAGGAAGGT CATAGTTTGG AGATGGAGAA TGAGAATCTT 
GTAGAAAATG GTGCAGACTC CGATGAAGAT GATAACAGCT TCCTCAAACA ACAATCTCCA 
CAAGAACCCA AGTCTCTGAA TTGGTCGAGT TTTGTAGACA ACACCTTTGC TGAAGAATTC 
ACTACTCAGA ATCAGAAATC CCAGGATGTG GAACTCTGGG AGGGAGAAGT GGTCAAAGAG 
CTCTCTGTGG AAGAACAGAT AAAGAGAAAT CGGTATTATG ATGAGGATGA GGATGAAGAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMA1 ( XM_017019430.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016874919.1 Homo sapiens LIM domain and actin binding 1 (LIMA1), transcript variant X3, mRNA.

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CloneID	OHu02323
Clone ID Related Accession (Same CDS sequence)	NM_001243775.1 , XM_017019430.1
Accession Version	XM_017019430.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1374bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	LIM domain and actin-binding protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCAAA AGGAGAATGT GCCCCCAGGT CCTGAGGTCT GCATCACCCA TCAGGAAGGG 
GAAAAGATTT CTGCAAATGA GAATAGCCTG GCAGTCCGTT CCACCCCTGC CGAAGATGAC 
TCCCGTGACT CCCAGGTTAA GAGTGAGGTT CAACAGCCTG TCCATCCCAA GCCACTAAGT 
CCAGATTCCA GAGCCTCCAG TCTTTCTGAA AGTTCTCCTC CCAAAGCAAT GAAGAAGTTT 
CAGGCACCTG CAAGAGAGAC CTGCGTGGAA TGTCAGAAGA CAGTCTATCC AATGGAGCGT 
CTCTTGGCCA ACCAGCAGGT GTTTCACATC AGCTGCTTCC GTTGCTCCTA TTGCAACAAC 
AAACTCAGTC TAGGAACATA TGCATCTTTA CATGGAAGAA TCTATTGTAA GCCTCACTTC 
AATCAACTCT TTAAATCTAA GGGCAACTAT GATGAAGGCT TTGGGCACAG ACCACACAAG 
GATCTATGGG CAAGCAAAAA TGAAAACGAA GAGATTTTGG AGAGACCAGC CCAGCTTGCA 
AATGCAAGGG AGACCCCTCA CAGCCCAGGG GTAGAAGATG CCCCTATTGC TAAGGTGGGT 
GTCCTGGCTG CAAGTATGGA AGCCAAGGCC TCCTCTCAGC AGGAGAAGGA AGACAAGCCA 
GCTGAAACCA AGAAGCTGAG GATCGCCTGG CCACCCCCCA CTGAACTTGG AAGTTCAGGA 
AGTGCCTTGG AGGAAGGGAT CAAAATGTCA AAGCCCAAAT GGCCTCCTGA AGACGAAATC 
AGCAAGCCCG AAGTTCCTGA GGATGTCGAT CTAGATCTGA AGAAGCTAAG ACGATCTTCT 
TCACTGAAGG AAAGAAGCCG CCCATTCACT GTAGCAGCTT CATTTCAAAG CACCTCTGTC 
AAGAGCCCAA AAACTGTGTC CCCACCTATC AGGAAAGGCT GGAGCATGTC AGAGCAGAGT 
GAAGAGTCTG TGGGTGGAAG AGTTGCAGAA AGGAAACAAG TGGAAAATGC CAAGGCTTCT 
AAGAAGAATG GGAATGTGGG AAAAACAACC TGGCAAAACA AAGAATCTAA AGGAGAGACA 
GGGAAGAGAA GTAAGGAAGG TCATAGTTTG GAGATGGAGA ATGAGAATCT TGTAGAAAAT 
GGTGCAGACT CCGATGAAGA TGATAACAGC TTCCTCAAAC AACAATCTCC ACAAGAACCC 
AAGTCTCTGA ATTGGTCGAG TTTTGTAGAC AACACCTTTG CTGAAGAATT CACTACTCAG 
AATCAGAAAT CCCAGGATGT GGAACTCTGG GAGGGAGAAG TGGTCAAAGA GCTCTCTGTG 
GAAGAACAGA TAAAGAGAAA TCGGTATTAT GATGAGGATG AGGATGAAGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016874919.1
CDS	1435..2808
Translation	MEQKENVPPGPEVCITHQEGEKISANENSLAVRSTPAEDDSRDSQVKSEVQQPVHPKPLSPDSRASSLSESSPPKAMKKFQAPARETCVECQKTVYPMERLLANQQVFHISCFRCSYCNNKLSLGTYASLHGRIYCKPHFNQLFKSKGNYDEGFGHRPHKDLWASKNENEEILERPAQLANARETPHSPGVEDAPIAKVGVLAASMEAKASSQQEKEDKPAETKKLRIAWPPPTELGSSGSALEEGIKMSKPKWPPEDEISKPEVPEDVDLDLKKLRRSSSLKERSRPFTVAASFQSTSVKSPKTVSPPIRKGWSMSEQSEESVGGRVAERKQVENAKASKKNGNVGKTTWQNKESKGETGKRSKEGHSLEMENENLVENGADSDEDDNSFLKQQSPQEPKSLNWSSFVDNTFAEEFTTQNQKSQDVELWEGEVVKELSVEEQIKRNRYYDEDEDEE

Target ORF information:

RefSeq Version	XM_017019430.1
Organism	Homo sapiens(human)
Definition	Homo sapiens LIM domain and actin binding 1 (LIMA1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017019430.1

ORF Insert Sequence:

ATGGAGCAAA AGGAGAATGT GCCCCCAGGT CCTGAGGTCT GCATCACCCA TCAGGAAGGG 
GAAAAGATTT CTGCAAATGA GAATAGCCTG GCAGTCCGTT CCACCCCTGC CGAAGATGAC 
TCCCGTGACT CCCAGGTTAA GAGTGAGGTT CAACAGCCTG TCCATCCCAA GCCACTAAGT 
CCAGATTCCA GAGCCTCCAG TCTTTCTGAA AGTTCTCCTC CCAAAGCAAT GAAGAAGTTT 
CAGGCACCTG CAAGAGAGAC CTGCGTGGAA TGTCAGAAGA CAGTCTATCC AATGGAGCGT 
CTCTTGGCCA ACCAGCAGGT GTTTCACATC AGCTGCTTCC GTTGCTCCTA TTGCAACAAC 
AAACTCAGTC TAGGAACATA TGCATCTTTA CATGGAAGAA TCTATTGTAA GCCTCACTTC 
AATCAACTCT TTAAATCTAA GGGCAACTAT GATGAAGGCT TTGGGCACAG ACCACACAAG 
GATCTATGGG CAAGCAAAAA TGAAAACGAA GAGATTTTGG AGAGACCAGC CCAGCTTGCA 
AATGCAAGGG AGACCCCTCA CAGCCCAGGG GTAGAAGATG CCCCTATTGC TAAGGTGGGT 
GTCCTGGCTG CAAGTATGGA AGCCAAGGCC TCCTCTCAGC AGGAGAAGGA AGACAAGCCA 
GCTGAAACCA AGAAGCTGAG GATCGCCTGG CCACCCCCCA CTGAACTTGG AAGTTCAGGA 
AGTGCCTTGG AGGAAGGGAT CAAAATGTCA AAGCCCAAAT GGCCTCCTGA AGACGAAATC 
AGCAAGCCCG AAGTTCCTGA GGATGTCGAT CTAGATCTGA AGAAGCTAAG ACGATCTTCT 
TCACTGAAGG AAAGAAGCCG CCCATTCACT GTAGCAGCTT CATTTCAAAG CACCTCTGTC 
AAGAGCCCAA AAACTGTGTC CCCACCTATC AGGAAAGGCT GGAGCATGTC AGAGCAGAGT 
GAAGAGTCTG TGGGTGGAAG AGTTGCAGAA AGGAAACAAG TGGAAAATGC CAAGGCTTCT 
AAGAAGAATG GGAATGTGGG AAAAACAACC TGGCAAAACA AAGAATCTAA AGGAGAGACA 
GGGAAGAGAA GTAAGGAAGG TCATAGTTTG GAGATGGAGA ATGAGAATCT TGTAGAAAAT 
GGTGCAGACT CCGATGAAGA TGATAACAGC TTCCTCAAAC AACAATCTCC ACAAGAACCC 
AAGTCTCTGA ATTGGTCGAG TTTTGTAGAC AACACCTTTG CTGAAGAATT CACTACTCAG 
AATCAGAAAT CCCAGGATGT GGAACTCTGG GAGGGAGAAG TGGTCAAAGA GCTCTCTGTG 
GAAGAACAGA TAAAGAGAAA TCGGTATTAT GATGAGGATG AGGATGAAGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMA1 ( XM_011538455.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011536757.1 Homo sapiens LIM domain and actin binding 1 (LIMA1), transcript variant X1, mRNA.

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CloneID	OHu18730
Clone ID Related Accession (Same CDS sequence)	NM_001113546.2 , XM_011538455.2 , NM_001113546.1
Accession Version	XM_011538455.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2283bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	LIM domain and actin-binding protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011538455.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAATCAT CTCCATTTAA TAGACGGCAA TGGACCTCAC TATCATTGAG GGTAACAGCC 
AAAGAACTTT CTCTTGTCAA CAAGAACAAG TCATCGGCTA TTGTGGAAAT ATTCTCCAAG 
TACCAGAAAG CAGCTGAAGA AACAAACATG GAGAAGAAGA GAAGTAACAC CGAAAATCTC 
TCCCAGCACT TTAGAAAGGG GACCCTGACT GTGTTAAAGA AGAAGTGGGA GAACCCAGGG 
CTGGGAGCAG AGTCTCACAC AGACTCTCTA CGGAACAGCA GCACTGAGAT TAGGCACAGA 
GCAGACCATC CTCCTGCTGA AGTGACAAGC CACGCTGCTT CTGGAGCCAA AGCTGACCAA 
GAAGAACAAA TCCACCCCAG ATCTAGACTC AGGTCACCTC CTGAAGCCCT CGTTCAGGGT 
CGATATCCCC ACATCAAGGA CGGTGAGGAT CTTAAAGACC ACTCAACAGA AAGTAAAAAA 
ATGGAAAATT GTCTAGGAGA ATCCAGGCAT GAAGTAGAAA AATCAGAAAT CAGTGAAAAC 
ACAGATGCTT CGGGCAAAAT AGAGAAATAT AATGTTCCGC TGAACAGGCT TAAGATGATG 
TTTGAGAAAG GTGAACCAAC TCAAACTAAG ATTCTCCGGG CCCAAAGCCG AAGTGCAAGT 
GGAAGGAAGA TCTCTGAAAA CAGCTATTCT CTAGATGACC TGGAAATAGG CCCAGGTCAG 
TTGTCATCTT CTACATTTGA CTCGGAGAAA AATGAGAGTA GACGAAATCT GGAACTTCCA 
CGCCTCTCAG AAACCTCTAT AAAGGATCGA ATGGCCAAGT ACCAGGCAGC TGTGTCCAAA 
CAAAGCAGCT CAACCAACTA TACAAATGAG CTGAAAGCCA GTGGTGGCGA AATCAAAATT 
CATAAAATGG AGCAAAAGGA GAATGTGCCC CCAGGTCCTG AGGTCTGCAT CACCCATCAG 
GAAGGGGAAA AGATTTCTGC AAATGAGAAT AGCCTGGCAG TCCGTTCCAC CCCTGCCGAA 
GATGACTCCC CAGGTGACTC CCAGGTTAAG AGTGAGGTTC AACAGCCTGT CCATCCCAAG 
CCACTAAGTC CAGATTCCAG AGCCTCCAGT CTTTCTGAAA GTTCTCCTCC CAAAGCAATG 
AAGAAGTTTC AGGCACCTGC AAGAGAGACC TGCGTGGAAT GTCAGAAGAC AGTCTATCCA 
ATGGAGCGTC TCTTGGCCAA CCAGCAGGTG TTTCACATCA GCTGCTTCCG TTGCTCCTAT 
TGCAACAACA AACTCAGTCT AGGAACATAT GCATCTTTAC ATGGAAGAAT CTATTGTAAG 
CCTCACTTCA ATCAACTCTT TAAATCTAAG GGCAACTATG ATGAAGGCTT TGGGCACAGA 
CCACACAAGG ATCTATGGGC AAGCAAAAAT GAAAACGAAG AGATTTTGGA GAGACCAGCC 
CAGCTTGCAA ATGCAAGGGA GACCCCTCAC AGCCCAGGGG TAGAAGATGC CCCTATTGCT 
AAGGTGGGTG TCCTGGCTGC AAGTATGGAA GCCAAGGCCT CCTCTCAGCA GGAGAAGGAA 
GACAAGCCAG CTGAAACCAA GAAGCTGAGG ATCGCCTGGC CACCCCCCAC TGAACTTGGA 
AGTTCAGGAA GTGCCTTGGA GGAAGGGATC AAAATGTCAA AGCCCAAATG GCCTCCTGAA 
GACGAAATCA GCAAGCCCGA AGTTCCTGAG GATGTCGATC TAGATCTGAA GAAGCTAAGA 
CGATCTTCTT CACTGAAGGA AAGAAGCCGC CCATTCACTG TAGCAGCTTC ATTTCAAAGC 
ACCTCTGTCA AGAGCCCAAA AACTGTGTCC CCACCTATCA GGAAAGGCTG GAGCATGTCA 
GAGCAGAGTG AAGAGTCTGT GGGTGGAAGA GTTGCAGAAA GGAAACAAGT GGAAAATGCC 
AAGGCTTCTA AGAAGAATGG GAATGTGGGA AAAACAACCT GGCAAAACAA AGAATCTAAA 
GGAGAGACAG GGAAGAGAAG TAAGGAAGGT CATAGTTTGG AGATGGAGAA TGAGAATCTT 
GTAGAAAATG GTGCAGACTC CGATGAAGAT GATAACAGCT TCCTCAAACA ACAATCTCCA 
CAAGAACCCA AGTCTCTGAA TTGGTCGAGT TTTGTAGACA ACACCTTTGC TGAAGAATTC 
ACTACTCAGA ATCAGAAATC CCAGGATGTG GAACTCTGGG AGGGAGAAGT GGTCAAAGAG 
CTCTCTGTGG AAGAACAGAT AAAGAGAAAT CGGTATTATG ATGAGGATGA GGATGAAGAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011536757.1
CDS	235..2517
Translation	MESSPFNRRQWTSLSLRVTAKELSLVNKNKSSAIVEIFSKYQKAAEETNMEKKRSNTENLSQHFRKGTLTVLKKKWENPGLGAESHTDSLRNSSTEIRHRADHPPAEVTSHAASGAKADQEEQIHPRSRLRSPPEALVQGRYPHIKDGEDLKDHSTESKKMENCLGESRHEVEKSEISENTDASGKIEKYNVPLNRLKMMFEKGEPTQTKILRAQSRSASGRKISENSYSLDDLEIGPGQLSSSTFDSEKNESRRNLELPRLSETSIKDRMAKYQAAVSKQSSSTNYTNELKASGGEIKIHKMEQKENVPPGPEVCITHQEGEKISANENSLAVRSTPAEDDSPGDSQVKSEVQQPVHPKPLSPDSRASSLSESSPPKAMKKFQAPARETCVECQKTVYPMERLLANQQVFHISCFRCSYCNNKLSLGTYASLHGRIYCKPHFNQLFKSKGNYDEGFGHRPHKDLWASKNENEEILERPAQLANARETPHSPGVEDAPIAKVGVLAASMEAKASSQQEKEDKPAETKKLRIAWPPPTELGSSGSALEEGIKMSKPKWPPEDEISKPEVPEDVDLDLKKLRRSSSLKERSRPFTVAASFQSTSVKSPKTVSPPIRKGWSMSEQSEESVGGRVAERKQVENAKASKKNGNVGKTTWQNKESKGETGKRSKEGHSLEMENENLVENGADSDEDDNSFLKQQSPQEPKSLNWSSFVDNTFAEEFTTQNQKSQDVELWEGEVVKELSVEEQIKRNRYYDEDEDEE

Target ORF information:

RefSeq Version	XM_011538455.2
Organism	Homo sapiens(human)
Definition	Homo sapiens LIM domain and actin binding 1 (LIMA1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011538455.2

ORF Insert Sequence:

ATGGAATCAT CTCCATTTAA TAGACGGCAA TGGACCTCAC TATCATTGAG GGTAACAGCC 
AAAGAACTTT CTCTTGTCAA CAAGAACAAG TCATCGGCTA TTGTGGAAAT ATTCTCCAAG 
TACCAGAAAG CAGCTGAAGA AACAAACATG GAGAAGAAGA GAAGTAACAC CGAAAATCTC 
TCCCAGCACT TTAGAAAGGG GACCCTGACT GTGTTAAAGA AGAAGTGGGA GAACCCAGGG 
CTGGGAGCAG AGTCTCACAC AGACTCTCTA CGGAACAGCA GCACTGAGAT TAGGCACAGA 
GCAGACCATC CTCCTGCTGA AGTGACAAGC CACGCTGCTT CTGGAGCCAA AGCTGACCAA 
GAAGAACAAA TCCACCCCAG ATCTAGACTC AGGTCACCTC CTGAAGCCCT CGTTCAGGGT 
CGATATCCCC ACATCAAGGA CGGTGAGGAT CTTAAAGACC ACTCAACAGA AAGTAAAAAA 
ATGGAAAATT GTCTAGGAGA ATCCAGGCAT GAAGTAGAAA AATCAGAAAT CAGTGAAAAC 
ACAGATGCTT CGGGCAAAAT AGAGAAATAT AATGTTCCGC TGAACAGGCT TAAGATGATG 
TTTGAGAAAG GTGAACCAAC TCAAACTAAG ATTCTCCGGG CCCAAAGCCG AAGTGCAAGT 
GGAAGGAAGA TCTCTGAAAA CAGCTATTCT CTAGATGACC TGGAAATAGG CCCAGGTCAG 
TTGTCATCTT CTACATTTGA CTCGGAGAAA AATGAGAGTA GACGAAATCT GGAACTTCCA 
CGCCTCTCAG AAACCTCTAT AAAGGATCGA ATGGCCAAGT ACCAGGCAGC TGTGTCCAAA 
CAAAGCAGCT CAACCAACTA TACAAATGAG CTGAAAGCCA GTGGTGGCGA AATCAAAATT 
CATAAAATGG AGCAAAAGGA GAATGTGCCC CCAGGTCCTG AGGTCTGCAT CACCCATCAG 
GAAGGGGAAA AGATTTCTGC AAATGAGAAT AGCCTGGCAG TCCGTTCCAC CCCTGCCGAA 
GATGACTCCC CAGGTGACTC CCAGGTTAAG AGTGAGGTTC AACAGCCTGT CCATCCCAAG 
CCACTAAGTC CAGATTCCAG AGCCTCCAGT CTTTCTGAAA GTTCTCCTCC CAAAGCAATG 
AAGAAGTTTC AGGCACCTGC AAGAGAGACC TGCGTGGAAT GTCAGAAGAC AGTCTATCCA 
ATGGAGCGTC TCTTGGCCAA CCAGCAGGTG TTTCACATCA GCTGCTTCCG TTGCTCCTAT 
TGCAACAACA AACTCAGTCT AGGAACATAT GCATCTTTAC ATGGAAGAAT CTATTGTAAG 
CCTCACTTCA ATCAACTCTT TAAATCTAAG GGCAACTATG ATGAAGGCTT TGGGCACAGA 
CCACACAAGG ATCTATGGGC AAGCAAAAAT GAAAACGAAG AGATTTTGGA GAGACCAGCC 
CAGCTTGCAA ATGCAAGGGA GACCCCTCAC AGCCCAGGGG TAGAAGATGC CCCTATTGCT 
AAGGTGGGTG TCCTGGCTGC AAGTATGGAA GCCAAGGCCT CCTCTCAGCA GGAGAAGGAA 
GACAAGCCAG CTGAAACCAA GAAGCTGAGG ATCGCCTGGC CACCCCCCAC TGAACTTGGA 
AGTTCAGGAA GTGCCTTGGA GGAAGGGATC AAAATGTCAA AGCCCAAATG GCCTCCTGAA 
GACGAAATCA GCAAGCCCGA AGTTCCTGAG GATGTCGATC TAGATCTGAA GAAGCTAAGA 
CGATCTTCTT CACTGAAGGA AAGAAGCCGC CCATTCACTG TAGCAGCTTC ATTTCAAAGC 
ACCTCTGTCA AGAGCCCAAA AACTGTGTCC CCACCTATCA GGAAAGGCTG GAGCATGTCA 
GAGCAGAGTG AAGAGTCTGT GGGTGGAAGA GTTGCAGAAA GGAAACAAGT GGAAAATGCC 
AAGGCTTCTA AGAAGAATGG GAATGTGGGA AAAACAACCT GGCAAAACAA AGAATCTAAA 
GGAGAGACAG GGAAGAGAAG TAAGGAAGGT CATAGTTTGG AGATGGAGAA TGAGAATCTT 
GTAGAAAATG GTGCAGACTC CGATGAAGAT GATAACAGCT TCCTCAAACA ACAATCTCCA 
CAAGAACCCA AGTCTCTGAA TTGGTCGAGT TTTGTAGACA ACACCTTTGC TGAAGAATTC 
ACTACTCAGA ATCAGAAATC CCAGGATGTG GAACTCTGGG AGGGAGAAGT GGTCAAAGAG 
CTCTCTGTGG AAGAACAGAT AAAGAGAAAT CGGTATTATG ATGAGGATGA GGATGAAGAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMA1 ( NM_001113547.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001107019.1 Homo sapiens LIM domain and actin binding 1 (LIMA1), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu06399
Clone ID Related Accession (Same CDS sequence)	NM_001113547.2 , NM_001113547.1
Accession Version	NM_001113547.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1803bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Homo sapiens(human)
Product	LIM domain and actin-binding protein 1 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA738195.1, AF198455.1, BC110815.1 and AL136911.1. On Jun 2, 2019 this sequence version replaced NM_001113547.1. Transcript Variant: This variant (3) differs at the 5' end compared to variant 1. This results in translation initiation from an in-frame downstream AUG, and an isoform (3, also known as EPLIN-alpha) with a shorter N-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF198455.1, SRR1803615.150737.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAAAATT GTCTAGGAGA ATCCAGGCAT GAAGTAGAAA AATCAGAAAT CAGTGAAAAC 
ACAGATGCTT CGGGCAAAAT AGAGAAATAT AATGTTCCGC TGAACAGGCT TAAGATGATG 
TTTGAGAAAG GTGAACCAAC TCAAACTAAG ATTCTCCGGG CCCAAAGCCG AAGTGCAAGT 
GGAAGGAAGA TCTCTGAAAA CAGCTATTCT CTAGATGACC TGGAAATAGG CCCAGGTCAG 
TTGTCATCTT CTACATTTGA CTCGGAGAAA AATGAGAGTA GACGAAATCT GGAACTTCCA 
CGCCTCTCAG AAACCTCTAT AAAGGATCGA ATGGCCAAGT ACCAGGCAGC TGTGTCCAAA 
CAAAGCAGCT CAACCAACTA TACAAATGAG CTGAAAGCCA GTGGTGGCGA AATCAAAATT 
CATAAAATGG AGCAAAAGGA GAATGTGCCC CCAGGTCCTG AGGTCTGCAT CACCCATCAG 
GAAGGGGAAA AGATTTCTGC AAATGAGAAT AGCCTGGCAG TCCGTTCCAC CCCTGCCGAA 
GATGACTCCC CAGGTGACTC CCAGGTTAAG AGTGAGGTTC AACAGCCTGT CCATCCCAAG 
CCACTAAGTC CAGATTCCAG AGCCTCCAGT CTTTCTGAAA GTTCTCCTCC CAAAGCAATG 
AAGAAGTTTC AGGCACCTGC AAGAGAGACC TGCGTGGAAT GTCAGAAGAC AGTCTATCCA 
ATGGAGCGTC TCTTGGCCAA CCAGCAGGTG TTTCACATCA GCTGCTTCCG TTGCTCCTAT 
TGCAACAACA AACTCAGTCT AGGAACATAT GCATCTTTAC ATGGAAGAAT CTATTGTAAG 
CCTCACTTCA ATCAACTCTT TAAATCTAAG GGCAACTATG ATGAAGGCTT TGGGCACAGA 
CCACACAAGG ATCTATGGGC AAGCAAAAAT GAAAACGAAG AGATTTTGGA GAGACCAGCC 
CAGCTTGCAA ATGCAAGGGA GACCCCTCAC AGCCCAGGGG TAGAAGATGC CCCTATTGCT 
AAGGTGGGTG TCCTGGCTGC AAGTATGGAA GCCAAGGCCT CCTCTCAGCA GGAGAAGGAA 
GACAAGCCAG CTGAAACCAA GAAGCTGAGG ATCGCCTGGC CACCCCCCAC TGAACTTGGA 
AGTTCAGGAA GTGCCTTGGA GGAAGGGATC AAAATGTCAA AGCCCAAATG GCCTCCTGAA 
GACGAAATCA GCAAGCCCGA AGTTCCTGAG GATGTCGATC TAGATCTGAA GAAGCTAAGA 
CGATCTTCTT CACTGAAGGA AAGAAGCCGC CCATTCACTG TAGCAGCTTC ATTTCAAAGC 
ACCTCTGTCA AGAGCCCAAA AACTGTGTCC CCACCTATCA GGAAAGGCTG GAGCATGTCA 
GAGCAGAGTG AAGAGTCTGT GGGTGGAAGA GTTGCAGAAA GGAAACAAGT GGAAAATGCC 
AAGGCTTCTA AGAAGAATGG GAATGTGGGA AAAACAACCT GGCAAAACAA AGAATCTAAA 
GGAGAGACAG GGAAGAGAAG TAAGGAAGGT CATAGTTTGG AGATGGAGAA TGAGAATCTT 
GTAGAAAATG GTGCAGACTC CGATGAAGAT GATAACAGCT TCCTCAAACA ACAATCTCCA 
CAAGAACCCA AGTCTCTGAA TTGGTCGAGT TTTGTAGACA ACACCTTTGC TGAAGAATTC 
ACTACTCAGA ATCAGAAATC CCAGGATGTG GAACTCTGGG AGGGAGAAGT GGTCAAAGAG 
CTCTCTGTGG AAGAACAGAT AAAGAGAAAT CGGTATTATG ATGAGGATGA GGATGAAGAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001107019.1
CDS	489..2291
Translation	MENCLGESRHEVEKSEISENTDASGKIEKYNVPLNRLKMMFEKGEPTQTKILRAQSRSASGRKISENSYSLDDLEIGPGQLSSSTFDSEKNESRRNLELPRLSETSIKDRMAKYQAAVSKQSSSTNYTNELKASGGEIKIHKMEQKENVPPGPEVCITHQEGEKISANENSLAVRSTPAEDDSPGDSQVKSEVQQPVHPKPLSPDSRASSLSESSPPKAMKKFQAPARETCVECQKTVYPMERLLANQQVFHISCFRCSYCNNKLSLGTYASLHGRIYCKPHFNQLFKSKGNYDEGFGHRPHKDLWASKNENEEILERPAQLANARETPHSPGVEDAPIAKVGVLAASMEAKASSQQEKEDKPAETKKLRIAWPPPTELGSSGSALEEGIKMSKPKWPPEDEISKPEVPEDVDLDLKKLRRSSSLKERSRPFTVAASFQSTSVKSPKTVSPPIRKGWSMSEQSEESVGGRVAERKQVENAKASKKNGNVGKTTWQNKESKGETGKRSKEGHSLEMENENLVENGADSDEDDNSFLKQQSPQEPKSLNWSSFVDNTFAEEFTTQNQKSQDVELWEGEVVKELSVEEQIKRNRYYDEDEDEE

Target ORF information:

RefSeq Version	NM_001113547.2
Organism	Homo sapiens(human)
Definition	Homo sapiens LIM domain and actin binding 1 (LIMA1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001113547.2

ORF Insert Sequence:

ATGGAAAATT GTCTAGGAGA ATCCAGGCAT GAAGTAGAAA AATCAGAAAT CAGTGAAAAC 
ACAGATGCTT CGGGCAAAAT AGAGAAATAT AATGTTCCGC TGAACAGGCT TAAGATGATG 
TTTGAGAAAG GTGAACCAAC TCAAACTAAG ATTCTCCGGG CCCAAAGCCG AAGTGCAAGT 
GGAAGGAAGA TCTCTGAAAA CAGCTATTCT CTAGATGACC TGGAAATAGG CCCAGGTCAG 
TTGTCATCTT CTACATTTGA CTCGGAGAAA AATGAGAGTA GACGAAATCT GGAACTTCCA 
CGCCTCTCAG AAACCTCTAT AAAGGATCGA ATGGCCAAGT ACCAGGCAGC TGTGTCCAAA 
CAAAGCAGCT CAACCAACTA TACAAATGAG CTGAAAGCCA GTGGTGGCGA AATCAAAATT 
CATAAAATGG AGCAAAAGGA GAATGTGCCC CCAGGTCCTG AGGTCTGCAT CACCCATCAG 
GAAGGGGAAA AGATTTCTGC AAATGAGAAT AGCCTGGCAG TCCGTTCCAC CCCTGCCGAA 
GATGACTCCC CAGGTGACTC CCAGGTTAAG AGTGAGGTTC AACAGCCTGT CCATCCCAAG 
CCACTAAGTC CAGATTCCAG AGCCTCCAGT CTTTCTGAAA GTTCTCCTCC CAAAGCAATG 
AAGAAGTTTC AGGCACCTGC AAGAGAGACC TGCGTGGAAT GTCAGAAGAC AGTCTATCCA 
ATGGAGCGTC TCTTGGCCAA CCAGCAGGTG TTTCACATCA GCTGCTTCCG TTGCTCCTAT 
TGCAACAACA AACTCAGTCT AGGAACATAT GCATCTTTAC ATGGAAGAAT CTATTGTAAG 
CCTCACTTCA ATCAACTCTT TAAATCTAAG GGCAACTATG ATGAAGGCTT TGGGCACAGA 
CCACACAAGG ATCTATGGGC AAGCAAAAAT GAAAACGAAG AGATTTTGGA GAGACCAGCC 
CAGCTTGCAA ATGCAAGGGA GACCCCTCAC AGCCCAGGGG TAGAAGATGC CCCTATTGCT 
AAGGTGGGTG TCCTGGCTGC AAGTATGGAA GCCAAGGCCT CCTCTCAGCA GGAGAAGGAA 
GACAAGCCAG CTGAAACCAA GAAGCTGAGG ATCGCCTGGC CACCCCCCAC TGAACTTGGA 
AGTTCAGGAA GTGCCTTGGA GGAAGGGATC AAAATGTCAA AGCCCAAATG GCCTCCTGAA 
GACGAAATCA GCAAGCCCGA AGTTCCTGAG GATGTCGATC TAGATCTGAA GAAGCTAAGA 
CGATCTTCTT CACTGAAGGA AAGAAGCCGC CCATTCACTG TAGCAGCTTC ATTTCAAAGC 
ACCTCTGTCA AGAGCCCAAA AACTGTGTCC CCACCTATCA GGAAAGGCTG GAGCATGTCA 
GAGCAGAGTG AAGAGTCTGT GGGTGGAAGA GTTGCAGAAA GGAAACAAGT GGAAAATGCC 
AAGGCTTCTA AGAAGAATGG GAATGTGGGA AAAACAACCT GGCAAAACAA AGAATCTAAA 
GGAGAGACAG GGAAGAGAAG TAAGGAAGGT CATAGTTTGG AGATGGAGAA TGAGAATCTT 
GTAGAAAATG GTGCAGACTC CGATGAAGAT GATAACAGCT TCCTCAAACA ACAATCTCCA 
CAAGAACCCA AGTCTCTGAA TTGGTCGAGT TTTGTAGACA ACACCTTTGC TGAAGAATTC 
ACTACTCAGA ATCAGAAATC CCAGGATGTG GAACTCTGGG AGGGAGAAGT GGTCAAAGAG 
CTCTCTGTGG AAGAACAGAT AAAGAGAAAT CGGTATTATG ATGAGGATGA GGATGAAGAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMA1 ( NM_001113547.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001107019.1 Homo sapiens LIM domain and actin binding 1 (LIMA1), transcript variant 3, mRNA.

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CloneID	OHu06399
Clone ID Related Accession (Same CDS sequence)	NM_001113547.2 , NM_001113547.1
Accession Version	NM_001113547.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1803bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-03-11
Organism	Homo sapiens(human)
Product	LIM domain and actin-binding protein 1 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA738195.1, AF198455.1, BC110815.1, AL136911.1 and AA772616.1. Transcript Variant: This variant (3) differs at the 5' end compared to variant 1. This results in translation initiation from an in-frame downstream AUG, and an isoform (3, also known as EPLIN-alpha) with a shorter N-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF198455.1, SRR1803615.150737.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAAAATT GTCTAGGAGA ATCCAGGCAT GAAGTAGAAA AATCAGAAAT CAGTGAAAAC 
ACAGATGCTT CGGGCAAAAT AGAGAAATAT AATGTTCCGC TGAACAGGCT TAAGATGATG 
TTTGAGAAAG GTGAACCAAC TCAAACTAAG ATTCTCCGGG CCCAAAGCCG AAGTGCAAGT 
GGAAGGAAGA TCTCTGAAAA CAGCTATTCT CTAGATGACC TGGAAATAGG CCCAGGTCAG 
TTGTCATCTT CTACATTTGA CTCGGAGAAA AATGAGAGTA GACGAAATCT GGAACTTCCA 
CGCCTCTCAG AAACCTCTAT AAAGGATCGA ATGGCCAAGT ACCAGGCAGC TGTGTCCAAA 
CAAAGCAGCT CAACCAACTA TACAAATGAG CTGAAAGCCA GTGGTGGCGA AATCAAAATT 
CATAAAATGG AGCAAAAGGA GAATGTGCCC CCAGGTCCTG AGGTCTGCAT CACCCATCAG 
GAAGGGGAAA AGATTTCTGC AAATGAGAAT AGCCTGGCAG TCCGTTCCAC CCCTGCCGAA 
GATGACTCCC CAGGTGACTC CCAGGTTAAG AGTGAGGTTC AACAGCCTGT CCATCCCAAG 
CCACTAAGTC CAGATTCCAG AGCCTCCAGT CTTTCTGAAA GTTCTCCTCC CAAAGCAATG 
AAGAAGTTTC AGGCACCTGC AAGAGAGACC TGCGTGGAAT GTCAGAAGAC AGTCTATCCA 
ATGGAGCGTC TCTTGGCCAA CCAGCAGGTG TTTCACATCA GCTGCTTCCG TTGCTCCTAT 
TGCAACAACA AACTCAGTCT AGGAACATAT GCATCTTTAC ATGGAAGAAT CTATTGTAAG 
CCTCACTTCA ATCAACTCTT TAAATCTAAG GGCAACTATG ATGAAGGCTT TGGGCACAGA 
CCACACAAGG ATCTATGGGC AAGCAAAAAT GAAAACGAAG AGATTTTGGA GAGACCAGCC 
CAGCTTGCAA ATGCAAGGGA GACCCCTCAC AGCCCAGGGG TAGAAGATGC CCCTATTGCT 
AAGGTGGGTG TCCTGGCTGC AAGTATGGAA GCCAAGGCCT CCTCTCAGCA GGAGAAGGAA 
GACAAGCCAG CTGAAACCAA GAAGCTGAGG ATCGCCTGGC CACCCCCCAC TGAACTTGGA 
AGTTCAGGAA GTGCCTTGGA GGAAGGGATC AAAATGTCAA AGCCCAAATG GCCTCCTGAA 
GACGAAATCA GCAAGCCCGA AGTTCCTGAG GATGTCGATC TAGATCTGAA GAAGCTAAGA 
CGATCTTCTT CACTGAAGGA AAGAAGCCGC CCATTCACTG TAGCAGCTTC ATTTCAAAGC 
ACCTCTGTCA AGAGCCCAAA AACTGTGTCC CCACCTATCA GGAAAGGCTG GAGCATGTCA 
GAGCAGAGTG AAGAGTCTGT GGGTGGAAGA GTTGCAGAAA GGAAACAAGT GGAAAATGCC 
AAGGCTTCTA AGAAGAATGG GAATGTGGGA AAAACAACCT GGCAAAACAA AGAATCTAAA 
GGAGAGACAG GGAAGAGAAG TAAGGAAGGT CATAGTTTGG AGATGGAGAA TGAGAATCTT 
GTAGAAAATG GTGCAGACTC CGATGAAGAT GATAACAGCT TCCTCAAACA ACAATCTCCA 
CAAGAACCCA AGTCTCTGAA TTGGTCGAGT TTTGTAGACA ACACCTTTGC TGAAGAATTC 
ACTACTCAGA ATCAGAAATC CCAGGATGTG GAACTCTGGG AGGGAGAAGT GGTCAAAGAG 
CTCTCTGTGG AAGAACAGAT AAAGAGAAAT CGGTATTATG ATGAGGATGA GGATGAAGAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001107019.1
CDS	536..2338
Translation	MENCLGESRHEVEKSEISENTDASGKIEKYNVPLNRLKMMFEKGEPTQTKILRAQSRSASGRKISENSYSLDDLEIGPGQLSSSTFDSEKNESRRNLELPRLSETSIKDRMAKYQAAVSKQSSSTNYTNELKASGGEIKIHKMEQKENVPPGPEVCITHQEGEKISANENSLAVRSTPAEDDSPGDSQVKSEVQQPVHPKPLSPDSRASSLSESSPPKAMKKFQAPARETCVECQKTVYPMERLLANQQVFHISCFRCSYCNNKLSLGTYASLHGRIYCKPHFNQLFKSKGNYDEGFGHRPHKDLWASKNENEEILERPAQLANARETPHSPGVEDAPIAKVGVLAASMEAKASSQQEKEDKPAETKKLRIAWPPPTELGSSGSALEEGIKMSKPKWPPEDEISKPEVPEDVDLDLKKLRRSSSLKERSRPFTVAASFQSTSVKSPKTVSPPIRKGWSMSEQSEESVGGRVAERKQVENAKASKKNGNVGKTTWQNKESKGETGKRSKEGHSLEMENENLVENGADSDEDDNSFLKQQSPQEPKSLNWSSFVDNTFAEEFTTQNQKSQDVELWEGEVVKELSVEEQIKRNRYYDEDEDEE

Target ORF information:

RefSeq Version	NM_001113547.1
Organism	Homo sapiens(human)
Definition	Homo sapiens LIM domain and actin binding 1 (LIMA1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001113547.1

ORF Insert Sequence:

ATGGAAAATT GTCTAGGAGA ATCCAGGCAT GAAGTAGAAA AATCAGAAAT CAGTGAAAAC 
ACAGATGCTT CGGGCAAAAT AGAGAAATAT AATGTTCCGC TGAACAGGCT TAAGATGATG 
TTTGAGAAAG GTGAACCAAC TCAAACTAAG ATTCTCCGGG CCCAAAGCCG AAGTGCAAGT 
GGAAGGAAGA TCTCTGAAAA CAGCTATTCT CTAGATGACC TGGAAATAGG CCCAGGTCAG 
TTGTCATCTT CTACATTTGA CTCGGAGAAA AATGAGAGTA GACGAAATCT GGAACTTCCA 
CGCCTCTCAG AAACCTCTAT AAAGGATCGA ATGGCCAAGT ACCAGGCAGC TGTGTCCAAA 
CAAAGCAGCT CAACCAACTA TACAAATGAG CTGAAAGCCA GTGGTGGCGA AATCAAAATT 
CATAAAATGG AGCAAAAGGA GAATGTGCCC CCAGGTCCTG AGGTCTGCAT CACCCATCAG 
GAAGGGGAAA AGATTTCTGC AAATGAGAAT AGCCTGGCAG TCCGTTCCAC CCCTGCCGAA 
GATGACTCCC CAGGTGACTC CCAGGTTAAG AGTGAGGTTC AACAGCCTGT CCATCCCAAG 
CCACTAAGTC CAGATTCCAG AGCCTCCAGT CTTTCTGAAA GTTCTCCTCC CAAAGCAATG 
AAGAAGTTTC AGGCACCTGC AAGAGAGACC TGCGTGGAAT GTCAGAAGAC AGTCTATCCA 
ATGGAGCGTC TCTTGGCCAA CCAGCAGGTG TTTCACATCA GCTGCTTCCG TTGCTCCTAT 
TGCAACAACA AACTCAGTCT AGGAACATAT GCATCTTTAC ATGGAAGAAT CTATTGTAAG 
CCTCACTTCA ATCAACTCTT TAAATCTAAG GGCAACTATG ATGAAGGCTT TGGGCACAGA 
CCACACAAGG ATCTATGGGC AAGCAAAAAT GAAAACGAAG AGATTTTGGA GAGACCAGCC 
CAGCTTGCAA ATGCAAGGGA GACCCCTCAC AGCCCAGGGG TAGAAGATGC CCCTATTGCT 
AAGGTGGGTG TCCTGGCTGC AAGTATGGAA GCCAAGGCCT CCTCTCAGCA GGAGAAGGAA 
GACAAGCCAG CTGAAACCAA GAAGCTGAGG ATCGCCTGGC CACCCCCCAC TGAACTTGGA 
AGTTCAGGAA GTGCCTTGGA GGAAGGGATC AAAATGTCAA AGCCCAAATG GCCTCCTGAA 
GACGAAATCA GCAAGCCCGA AGTTCCTGAG GATGTCGATC TAGATCTGAA GAAGCTAAGA 
CGATCTTCTT CACTGAAGGA AAGAAGCCGC CCATTCACTG TAGCAGCTTC ATTTCAAAGC 
ACCTCTGTCA AGAGCCCAAA AACTGTGTCC CCACCTATCA GGAAAGGCTG GAGCATGTCA 
GAGCAGAGTG AAGAGTCTGT GGGTGGAAGA GTTGCAGAAA GGAAACAAGT GGAAAATGCC 
AAGGCTTCTA AGAAGAATGG GAATGTGGGA AAAACAACCT GGCAAAACAA AGAATCTAAA 
GGAGAGACAG GGAAGAGAAG TAAGGAAGGT CATAGTTTGG AGATGGAGAA TGAGAATCTT 
GTAGAAAATG GTGCAGACTC CGATGAAGAT GATAACAGCT TCCTCAAACA ACAATCTCCA 
CAAGAACCCA AGTCTCTGAA TTGGTCGAGT TTTGTAGACA ACACCTTTGC TGAAGAATTC 
ACTACTCAGA ATCAGAAATC CCAGGATGTG GAACTCTGGG AGGGAGAAGT GGTCAAAGAG 
CTCTCTGTGG AAGAACAGAT AAAGAGAAAT CGGTATTATG ATGAGGATGA GGATGAAGAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMA1 ( XM_017019429.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016874918.1 Homo sapiens LIM domain and actin binding 1 (LIMA1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu83858
Clone ID Related Accession (Same CDS sequence)	XM_017019429.1
Accession Version	XM_017019429.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1377bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	LIM domain and actin-binding protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCAAA AGGAGAATGT GCCCCCAGGT CCTGAGGTCT GCATCACCCA TCAGGAAGGG 
GAAAAGATTT CTGCAAATGA GAATAGCCTG GCAGTCCGTT CCACCCCTGC CGAAGATGAC 
TCCCCAGGTG ACTCCCAGGT TAAGAGTGAG GTTCAACAGC CTGTCCATCC CAAGCCACTA 
AGTCCAGATT CCAGAGCCTC CAGTCTTTCT GAAAGTTCTC CTCCCAAAGC AATGAAGAAG 
TTTCAGGCAC CTGCAAGAGA GACCTGCGTG GAATGTCAGA AGACAGTCTA TCCAATGGAG 
CGTCTCTTGG CCAACCAGCA GGTGTTTCAC ATCAGCTGCT TCCGTTGCTC CTATTGCAAC 
AACAAACTCA GTCTAGGAAC ATATGCATCT TTACATGGAA GAATCTATTG TAAGCCTCAC 
TTCAATCAAC TCTTTAAATC TAAGGGCAAC TATGATGAAG GCTTTGGGCA CAGACCACAC 
AAGGATCTAT GGGCAAGCAA AAATGAAAAC GAAGAGATTT TGGAGAGACC AGCCCAGCTT 
GCAAATGCAA GGGAGACCCC TCACAGCCCA GGGGTAGAAG ATGCCCCTAT TGCTAAGGTG 
GGTGTCCTGG CTGCAAGTAT GGAAGCCAAG GCCTCCTCTC AGCAGGAGAA GGAAGACAAG 
CCAGCTGAAA CCAAGAAGCT GAGGATCGCC TGGCCACCCC CCACTGAACT TGGAAGTTCA 
GGAAGTGCCT TGGAGGAAGG GATCAAAATG TCAAAGCCCA AATGGCCTCC TGAAGACGAA 
ATCAGCAAGC CCGAAGTTCC TGAGGATGTC GATCTAGATC TGAAGAAGCT AAGACGATCT 
TCTTCACTGA AGGAAAGAAG CCGCCCATTC ACTGTAGCAG CTTCATTTCA AAGCACCTCT 
GTCAAGAGCC CAAAAACTGT GTCCCCACCT ATCAGGAAAG GCTGGAGCAT GTCAGAGCAG 
AGTGAAGAGT CTGTGGGTGG AAGAGTTGCA GAAAGGAAAC AAGTGGAAAA TGCCAAGGCT 
TCTAAGAAGA ATGGGAATGT GGGAAAAACA ACCTGGCAAA ACAAAGAATC TAAAGGAGAG 
ACAGGGAAGA GAAGTAAGGA AGGTCATAGT TTGGAGATGG AGAATGAGAA TCTTGTAGAA 
AATGGTGCAG ACTCCGATGA AGATGATAAC AGCTTCCTCA AACAACAATC TCCACAAGAA 
CCCAAGTCTC TGAATTGGTC GAGTTTTGTA GACAACACCT TTGCTGAAGA ATTCACTACT 
CAGAATCAGA AATCCCAGGA TGTGGAACTC TGGGAGGGAG AAGTGGTCAA AGAGCTCTCT 
GTGGAAGAAC AGATAAAGAG AAATCGGTAT TATGATGAGG ATGAGGATGA AGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016874918.1
CDS	591..1967
Translation	MEQKENVPPGPEVCITHQEGEKISANENSLAVRSTPAEDDSPGDSQVKSEVQQPVHPKPLSPDSRASSLSESSPPKAMKKFQAPARETCVECQKTVYPMERLLANQQVFHISCFRCSYCNNKLSLGTYASLHGRIYCKPHFNQLFKSKGNYDEGFGHRPHKDLWASKNENEEILERPAQLANARETPHSPGVEDAPIAKVGVLAASMEAKASSQQEKEDKPAETKKLRIAWPPPTELGSSGSALEEGIKMSKPKWPPEDEISKPEVPEDVDLDLKKLRRSSSLKERSRPFTVAASFQSTSVKSPKTVSPPIRKGWSMSEQSEESVGGRVAERKQVENAKASKKNGNVGKTTWQNKESKGETGKRSKEGHSLEMENENLVENGADSDEDDNSFLKQQSPQEPKSLNWSSFVDNTFAEEFTTQNQKSQDVELWEGEVVKELSVEEQIKRNRYYDEDEDEE

Target ORF information:

RefSeq Version	XM_017019429.1
Organism	Homo sapiens(human)
Definition	Homo sapiens LIM domain and actin binding 1 (LIMA1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017019429.1

ORF Insert Sequence:

ATGGAGCAAA AGGAGAATGT GCCCCCAGGT CCTGAGGTCT GCATCACCCA TCAGGAAGGG 
GAAAAGATTT CTGCAAATGA GAATAGCCTG GCAGTCCGTT CCACCCCTGC CGAAGATGAC 
TCCCCAGGTG ACTCCCAGGT TAAGAGTGAG GTTCAACAGC CTGTCCATCC CAAGCCACTA 
AGTCCAGATT CCAGAGCCTC CAGTCTTTCT GAAAGTTCTC CTCCCAAAGC AATGAAGAAG 
TTTCAGGCAC CTGCAAGAGA GACCTGCGTG GAATGTCAGA AGACAGTCTA TCCAATGGAG 
CGTCTCTTGG CCAACCAGCA GGTGTTTCAC ATCAGCTGCT TCCGTTGCTC CTATTGCAAC 
AACAAACTCA GTCTAGGAAC ATATGCATCT TTACATGGAA GAATCTATTG TAAGCCTCAC 
TTCAATCAAC TCTTTAAATC TAAGGGCAAC TATGATGAAG GCTTTGGGCA CAGACCACAC 
AAGGATCTAT GGGCAAGCAA AAATGAAAAC GAAGAGATTT TGGAGAGACC AGCCCAGCTT 
GCAAATGCAA GGGAGACCCC TCACAGCCCA GGGGTAGAAG ATGCCCCTAT TGCTAAGGTG 
GGTGTCCTGG CTGCAAGTAT GGAAGCCAAG GCCTCCTCTC AGCAGGAGAA GGAAGACAAG 
CCAGCTGAAA CCAAGAAGCT GAGGATCGCC TGGCCACCCC CCACTGAACT TGGAAGTTCA 
GGAAGTGCCT TGGAGGAAGG GATCAAAATG TCAAAGCCCA AATGGCCTCC TGAAGACGAA 
ATCAGCAAGC CCGAAGTTCC TGAGGATGTC GATCTAGATC TGAAGAAGCT AAGACGATCT 
TCTTCACTGA AGGAAAGAAG CCGCCCATTC ACTGTAGCAG CTTCATTTCA AAGCACCTCT 
GTCAAGAGCC CAAAAACTGT GTCCCCACCT ATCAGGAAAG GCTGGAGCAT GTCAGAGCAG 
AGTGAAGAGT CTGTGGGTGG AAGAGTTGCA GAAAGGAAAC AAGTGGAAAA TGCCAAGGCT 
TCTAAGAAGA ATGGGAATGT GGGAAAAACA ACCTGGCAAA ACAAAGAATC TAAAGGAGAG 
ACAGGGAAGA GAAGTAAGGA AGGTCATAGT TTGGAGATGG AGAATGAGAA TCTTGTAGAA 
AATGGTGCAG ACTCCGATGA AGATGATAAC AGCTTCCTCA AACAACAATC TCCACAAGAA 
CCCAAGTCTC TGAATTGGTC GAGTTTTGTA GACAACACCT TTGCTGAAGA ATTCACTACT 
CAGAATCAGA AATCCCAGGA TGTGGAACTC TGGGAGGGAG AAGTGGTCAA AGAGCTCTCT 
GTGGAAGAAC AGATAAAGAG AAATCGGTAT TATGATGAGG ATGAGGATGA AGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	LIMA1
Entrez Gene ID	51474
Full Name	LIM domain and actin binding 1
Synonyms	EPLIN,SREBP3
General protein information	Preferred Names LIM domain and actin binding 1 Names LIM domain and actin-binding protein 1 1110021C24Rik epithelial protein lost in neoplasm beta sterol regulatory element binding protein 3
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 12 Map Location: 12q13.12
Summary	This gene encodes a cytoskeleton-associated protein that inhibits actin filament depolymerization and cross-links filaments in bundles. It is downregulated in some cancer cell lines. Alternatively spliced transcript variants encoding different isoforms have been described for this gene, and expression of some of the variants maybe independently regulated. [provided by RefSeq, Aug 2011].
Disorder MIM:	608364

Canis lupus familiaris (dog)	LIMA1	XP_005636908.1
Pan troglodytes (chimpanzee)	LIMA1	XP_001154716.2
Mus musculus (house mouse)	Lima1	NP_001107017.1
Xenopus tropicalis (tropical clawed frog)	lima1	NP_001072345.1
Gallus gallus (chicken)	LIMA1	XP_004949785.1
Homo sapiens (human)	LIMA1	NP_057441.1
	LIMA1	XP_001102909.1
Bos taurus (cattle)	LIMA1	NP_001179683.1
Rattus norvegicus (Norway rat)	Lima1	NP_001178544.1
Danio rerio (zebrafish)	im:7155904	XP_005172858.1

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LIMA1 cDNA ORF clone, Homo sapiens(human)

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gRNAs

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