SPART cDNA ORF clone, Canis lupus familiaris(dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	SPART
Entrez Gene ID	477303
Full Name	spartin
Synonyms	SPG20
General protein information	Preferred Names spartin Names spartin spastic paraplegia 20 (Troyer syndrome)
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005635459.3	XP_005635516.1	spartin
XM_005635459.2	XP_005635516.1	spartin
XM_014107428.1	XP_013962903.1	spartin
XM_014107427.1	XP_013962902.1	spartin
XM_022409585.1	XP_022265293.1	spartin
XM_014107426.1	XP_013962901.1	spartin
XM_014107426.2	XP_013962901.1	spartin

Pathways from BioSystems

Homologies

	SPG20	XP_001083494.1
Canis lupus familiaris (dog)	SPG20	XP_005635515.1
Gallus gallus (chicken)	SPG20	XP_004938806.1
Rattus norvegicus (Norway rat)	Spg20	NP_001099903.2
Danio rerio (zebrafish)	spg20a	NP_001107130.1
Caenorhabditis elegans (roundworm)	F57B10.9	NP_491888.2
Xenopus tropicalis (tropical clawed frog)	spg20	NP_001123727.1
Homo sapiens (human)	SPG20	NP_001135766.1
Bos taurus (cattle)	SPG20	NP_001071464.1
Pan troglodytes (chimpanzee)	SPG20	XP_003314174.1

Gene Ontology

The following SPART gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPART cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe25372	XM_005635459.3 Latest version!	Canis lupus familiaris spartin (SPART), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OCe25372	XM_022409585.1 Latest version!	Canis lupus familiaris spartin (SPART), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OCe25372	XM_014107426.2 Latest version!	Canis lupus familiaris spartin (SPART), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OCe25372	XM_014107428.1 Latest version!	Canis lupus familiaris spastic paraplegia 20 (Troyer syndrome) (SPG20), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OCe25372	XM_014107427.1 Latest version!	Canis lupus familiaris spastic paraplegia 20 (Troyer syndrome) (SPG20), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
View Old Accession Versions >>
OCe25372	XM_005635459.2	Canis lupus familiaris spastic paraplegia 20 (Troyer syndrome) (SPG20), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OCe25372	XM_014107426.1	Canis lupus familiaris spastic paraplegia 20 (Troyer syndrome) (SPG20), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SPART ( XM_005635459.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005635516.1 Canis lupus familiaris spartin (SPART), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe25372
Clone ID Related Accession (Same CDS sequence)	XM_005635459.3 , XM_005635459.2 , XM_014107428.1 , XM_014107427.1 , XM_022409585.1 , XM_014107426.1 , XM_014107426.2
Accession Version	XM_005635459.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2019bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	spartin
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006607.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005635459.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCAAG AGCCACAAAA TGGAGAACCT GCTGAAATTA AGATCATCAA GGAAGCATAC 
AAGAAAGCCT TTTTATTTGT TAATAAAGGA CTGAATACAG ATGAATTAGG TCAGAAGGAA 
GAAGCAAAGA GCTACTATAA GCAAGGAATA GGACATCTGC TCAGAGGAAT CAGCATATCA 
TCAACAGAGC CTGAACACAT AGGTCCTGGG TGGGAATCTG CTAGACAGAT GCAACAGAAA 
ATGAAAGAAA CACTACAGAA TGTACGTACC AGGTTGGAAA TTCTGGAGAA GGGTCTTGCC 
ACTTCTCTAC GGAATGATCT TCAGGAGGTG CCCAAGTTAT ATCCAGAATT TCCACCTAAA 
GACATGTCTG AAAAGTCACC AGAGCCTCAG TCCTTTAGTT CACCTCACCA GCATGCTGAA 
GTAAATGGAA ACACTTCAAC GACAAGCACA GGGTTGGTTC CTGCACCTAG TTCTTTATCC 
ATACCATCTC AAAGTCATCC AGCAGAAGCA CCTCCTGCTT ATACACCTCA GGCTGCTGAG 
GGCCACTACA CTGTATCCTA TGGAACAGAA TCTGGGGAAT TTTCGTCAGT TGGAGAAGAC 
TTTTACAGGA ATCATTCCCA GCCACCTCCT CTTGAAACCT TAGGGCTAGA TGCAGATGAG 
TTGATTTTGA TACCAAATGG AGTACAGATT TTTTTTGTAA ATCCTGCAGG GGAGGTTAGT 
GCACCTTCAT ATCCAGGGTA CCTTCGAATT GTGAGGTTCT TGGATAATTC TCTTGATACT 
GTTCTAAACC GTCCTCCTGG GTTTCTTCAG GTTTGTGACT GGTTATATCC TCTAGTTCCT 
GATAGATCTC CAGTTCTTAA ATGTACTGTG GGGGCCTACA TGTTTCCTGA TACCATGTTA 
CAAGCATCAG GATGTTTTGT GGGGGTCGTC TTGTCTTCTG AATTACCAGA AGATGATAGA 
GAACTCTTTG AGGATTTACT AAGACAGATG TCTGACCTTC GGCTTCAGGC CAACTGGAAC 
CGGGCAGAAG GAGAAAATGA ATTCCAAATT CCTGGAAGAG CAAGATGCAC CTCTGACCAA 
TTGAAGGAAG CCAGTGGCAC TGATGTGAGA CATTTGGGTC CTTCATTGGA CCAAGACAAT 
AAGGATGTGC GTCATAAAGG AAAACGGGGG AAAAAAGCCA AAGACACTTC AAGTGAAGAA 
GTTAATCTGA GTCACATTGT ACCCTATGAG CCAGTTTCAG AAGAAAAAGC AAAGGAATTA 
CCTGAATGGA GTGAGAAAGT GGCTCAAAAT ATTTTGTCAG GTGCTTCCTG GGTGAGCTGG 
GGTTTGGTCA AAGGTGCTGA ATTTACTGGC AAAGCAATCC AGAAAGGCGC TTCTAAACTC 
CGTGAACGGA TTCAACCAGA AGAAAAACCA GTGGAAGTTA GTCCAGCTGT GACCAGGGGA 
CTTTATATAG CAAAGCAGGC TACAGGAGGA GCAGCGAAAG TCAGTCAGTT CCTGGTTGAT 
GGAGTTTGCA CTGTAGCAAA TTGTGTTGGA AAGGAACTAG CTCCACATGT CAAGAAGCAT 
GGAAGCAAAC TTGTTCCAGA ATCTCTTAAA AAAGACAGAA ATGGAAAGTC TACCTTGGAT 
GGTGCTATGG TTGTAGCAGC AAGTAGTGTT CAAGGATTTT CAACTGTCTG GCAGGGATTG 
GAATGTGCAG CCAAATGTAT TGTTAATAAT GTTTCAGCAG AAACTGTACA AACTGTCAGA 
TACAAATACG GGCATACTGC AGGAGAAGCT ACACATGATG CAGTAGATTC TGCCATCAAT 
GTTGGTGTAA CTGCCTATAA TATTGATAAC ATTGGTATCA AAGCAATGGT GAAGAAAACT 
GCAAAAGAAA CAGGACAGAC ACTCCTGGAA GACTATAAAA TAGTTGATAA TTCTAAGGGG 
AGTAATCAAG AAGGAAGAGC AAATGTAAAC ATGAAAAGGG AGACGGGTGA GCAGACAAAG 
GACCTAGAGA AGAGTGAGGC AAAGAAGAAA GAGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005635516.1
CDS	152..2170
Translation	MEQEPQNGEPAEIKIIKEAYKKAFLFVNKGLNTDELGQKEEAKSYYKQGIGHLLRGISISSTEPEHIGPGWESARQMQQKMKETLQNVRTRLEILEKGLATSLRNDLQEVPKLYPEFPPKDMSEKSPEPQSFSSPHQHAEVNGNTSTTSTGLVPAPSSLSIPSQSHPAEAPPAYTPQAAEGHYTVSYGTESGEFSSVGEDFYRNHSQPPPLETLGLDADELILIPNGVQIFFVNPAGEVSAPSYPGYLRIVRFLDNSLDTVLNRPPGFLQVCDWLYPLVPDRSPVLKCTVGAYMFPDTMLQASGCFVGVVLSSELPEDDRELFEDLLRQMSDLRLQANWNRAEGENEFQIPGRARCTSDQLKEASGTDVRHLGPSLDQDNKDVRHKGKRGKKAKDTSSEEVNLSHIVPYEPVSEEKAKELPEWSEKVAQNILSGASWVSWGLVKGAEFTGKAIQKGASKLRERIQPEEKPVEVSPAVTRGLYIAKQATGGAAKVSQFLVDGVCTVANCVGKELAPHVKKHGSKLVPESLKKDRNGKSTLDGAMVVAASSVQGFSTVWQGLECAAKCIVNNVSAETVQTVRYKYGHTAGEATHDAVDSAINVGVTAYNIDNIGIKAMVKKTAKETGQTLLEDYKIVDNSKGSNQEGRANVNMKRETGEQTKDLEKSEAKKKEK

Target ORF information:

RefSeq Version	XM_005635459.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris spartin (SPART), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005635459.3

ORF Insert Sequence:

ATGGAGCAAG AGCCACAAAA TGGAGAACCT GCTGAAATTA AGATCATCAA GGAAGCATAC 
AAGAAAGCCT TTTTATTTGT TAATAAAGGA CTGAATACAG ATGAATTAGG TCAGAAGGAA 
GAAGCAAAGA GCTACTATAA GCAAGGAATA GGACATCTGC TCAGAGGAAT CAGCATATCA 
TCAACAGAGC CTGAACACAT AGGTCCTGGG TGGGAATCTG CTAGACAGAT GCAACAGAAA 
ATGAAAGAAA CACTACAGAA TGTACGTACC AGGTTGGAAA TTCTGGAGAA GGGTCTTGCC 
ACTTCTCTAC GGAATGATCT TCAGGAGGTG CCCAAGTTAT ATCCAGAATT TCCACCTAAA 
GACATGTCTG AAAAGTCACC AGAGCCTCAG TCCTTTAGTT CACCTCACCA GCATGCTGAA 
GTAAATGGAA ACACTTCAAC GACAAGCACA GGGTTGGTTC CTGCACCTAG TTCTTTATCC 
ATACCATCTC AAAGTCATCC AGCAGAAGCA CCTCCTGCTT ATACACCTCA GGCTGCTGAG 
GGCCACTACA CTGTATCCTA TGGAACAGAA TCTGGGGAAT TTTCGTCAGT TGGAGAAGAC 
TTTTACAGGA ATCATTCCCA GCCACCTCCT CTTGAAACCT TAGGGCTAGA TGCAGATGAG 
TTGATTTTGA TACCAAATGG AGTACAGATT TTTTTTGTAA ATCCTGCAGG GGAGGTTAGT 
GCACCTTCAT ATCCAGGGTA CCTTCGAATT GTGAGGTTCT TGGATAATTC TCTTGATACT 
GTTCTAAACC GTCCTCCTGG GTTTCTTCAG GTTTGTGACT GGTTATATCC TCTAGTTCCT 
GATAGATCTC CAGTTCTTAA ATGTACTGTG GGGGCCTACA TGTTTCCTGA TACCATGTTA 
CAAGCATCAG GATGTTTTGT GGGGGTCGTC TTGTCTTCTG AATTACCAGA AGATGATAGA 
GAACTCTTTG AGGATTTACT AAGACAGATG TCTGACCTTC GGCTTCAGGC CAACTGGAAC 
CGGGCAGAAG GAGAAAATGA ATTCCAAATT CCTGGAAGAG CAAGATGCAC CTCTGACCAA 
TTGAAGGAAG CCAGTGGCAC TGATGTGAGA CATTTGGGTC CTTCATTGGA CCAAGACAAT 
AAGGATGTGC GTCATAAAGG AAAACGGGGG AAAAAAGCCA AAGACACTTC AAGTGAAGAA 
GTTAATCTGA GTCACATTGT ACCCTATGAG CCAGTTTCAG AAGAAAAAGC AAAGGAATTA 
CCTGAATGGA GTGAGAAAGT GGCTCAAAAT ATTTTGTCAG GTGCTTCCTG GGTGAGCTGG 
GGTTTGGTCA AAGGTGCTGA ATTTACTGGC AAAGCAATCC AGAAAGGCGC TTCTAAACTC 
CGTGAACGGA TTCAACCAGA AGAAAAACCA GTGGAAGTTA GTCCAGCTGT GACCAGGGGA 
CTTTATATAG CAAAGCAGGC TACAGGAGGA GCAGCGAAAG TCAGTCAGTT CCTGGTTGAT 
GGAGTTTGCA CTGTAGCAAA TTGTGTTGGA AAGGAACTAG CTCCACATGT CAAGAAGCAT 
GGAAGCAAAC TTGTTCCAGA ATCTCTTAAA AAAGACAGAA ATGGAAAGTC TACCTTGGAT 
GGTGCTATGG TTGTAGCAGC AAGTAGTGTT CAAGGATTTT CAACTGTCTG GCAGGGATTG 
GAATGTGCAG CCAAATGTAT TGTTAATAAT GTTTCAGCAG AAACTGTACA AACTGTCAGA 
TACAAATACG GGCATACTGC AGGAGAAGCT ACACATGATG CAGTAGATTC TGCCATCAAT 
GTTGGTGTAA CTGCCTATAA TATTGATAAC ATTGGTATCA AAGCAATGGT GAAGAAAACT 
GCAAAAGAAA CAGGACAGAC ACTCCTGGAA GACTATAAAA TAGTTGATAA TTCTAAGGGG 
AGTAATCAAG AAGGAAGAGC AAATGTAAAC ATGAAAAGGG AGACGGGTGA GCAGACAAAG 
GACCTAGAGA AGAGTGAGGC AAAGAAGAAA GAGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPART ( XM_005635459.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005635516.1 Canis lupus familiaris spastic paraplegia 20 (Troyer syndrome) (SPG20), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe25372
Clone ID Related Accession (Same CDS sequence)	XM_005635459.3 , XM_005635459.2 , XM_014107428.1 , XM_014107427.1 , XM_022409585.1 , XM_014107426.1 , XM_014107426.2
Accession Version	XM_005635459.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2019bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	spartin
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726102.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005635459.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCAAG AGCCACAAAA TGGAGAACCT GCTGAAATTA AGATCATCAA GGAAGCATAC 
AAGAAAGCCT TTTTATTTGT TAATAAAGGA CTGAATACAG ATGAATTAGG TCAGAAGGAA 
GAAGCAAAGA GCTACTATAA GCAAGGAATA GGACATCTGC TCAGAGGAAT CAGCATATCA 
TCAACAGAGC CTGAACACAT AGGTCCTGGG TGGGAATCTG CTAGACAGAT GCAACAGAAA 
ATGAAAGAAA CACTACAGAA TGTACGTACC AGGTTGGAAA TTCTGGAGAA GGGTCTTGCC 
ACTTCTCTAC GGAATGATCT TCAGGAGGTG CCCAAGTTAT ATCCAGAATT TCCACCTAAA 
GACATGTCTG AAAAGTCACC AGAGCCTCAG TCCTTTAGTT CACCTCACCA GCATGCTGAA 
GTAAATGGAA ACACTTCAAC GACAAGCACA GGGTTGGTTC CTGCACCTAG TTCTTTATCC 
ATACCATCTC AAAGTCATCC AGCAGAAGCA CCTCCTGCTT ATACACCTCA GGCTGCTGAG 
GGCCACTACA CTGTATCCTA TGGAACAGAA TCTGGGGAAT TTTCGTCAGT TGGAGAAGAC 
TTTTACAGGA ATCATTCCCA GCCACCTCCT CTTGAAACCT TAGGGCTAGA TGCAGATGAG 
TTGATTTTGA TACCAAATGG AGTACAGATT TTTTTTGTAA ATCCTGCAGG GGAGGTTAGT 
GCACCTTCAT ATCCAGGGTA CCTTCGAATT GTGAGGTTCT TGGATAATTC TCTTGATACT 
GTTCTAAACC GTCCTCCTGG GTTTCTTCAG GTTTGTGACT GGTTATATCC TCTAGTTCCT 
GATAGATCTC CAGTTCTTAA ATGTACTGTG GGGGCCTACA TGTTTCCTGA TACCATGTTA 
CAAGCATCAG GATGTTTTGT GGGGGTCGTC TTGTCTTCTG AATTACCAGA AGATGATAGA 
GAACTCTTTG AGGATTTACT AAGACAGATG TCTGACCTTC GGCTTCAGGC CAACTGGAAC 
CGGGCAGAAG GAGAAAATGA ATTCCAAATT CCTGGAAGAG CAAGATGCAC CTCTGACCAA 
TTGAAGGAAG CCAGTGGCAC TGATGTGAGA CATTTGGGTC CTTCATTGGA CCAAGACAAT 
AAGGATGTGC GTCATAAAGG AAAACGGGGG AAAAAAGCCA AAGACACTTC AAGTGAAGAA 
GTTAATCTGA GTCACATTGT ACCCTATGAG CCAGTTTCAG AAGAAAAAGC AAAGGAATTA 
CCTGAATGGA GTGAGAAAGT GGCTCAAAAT ATTTTGTCAG GTGCTTCCTG GGTGAGCTGG 
GGTTTGGTCA AAGGTGCTGA ATTTACTGGC AAAGCAATCC AGAAAGGCGC TTCTAAACTC 
CGTGAACGGA TTCAACCAGA AGAAAAACCA GTGGAAGTTA GTCCAGCTGT GACCAGGGGA 
CTTTATATAG CAAAGCAGGC TACAGGAGGA GCAGCGAAAG TCAGTCAGTT CCTGGTTGAT 
GGAGTTTGCA CTGTAGCAAA TTGTGTTGGA AAGGAACTAG CTCCACATGT CAAGAAGCAT 
GGAAGCAAAC TTGTTCCAGA ATCTCTTAAA AAAGACAGAA ATGGAAAGTC TACCTTGGAT 
GGTGCTATGG TTGTAGCAGC AAGTAGTGTT CAAGGATTTT CAACTGTCTG GCAGGGATTG 
GAATGTGCAG CCAAATGTAT TGTTAATAAT GTTTCAGCAG AAACTGTACA AACTGTCAGA 
TACAAATACG GGCATACTGC AGGAGAAGCT ACACATGATG CAGTAGATTC TGCCATCAAT 
GTTGGTGTAA CTGCCTATAA TATTGATAAC ATTGGTATCA AAGCAATGGT GAAGAAAACT 
GCAAAAGAAA CAGGACAGAC ACTCCTGGAA GACTATAAAA TAGTTGATAA TTCTAAGGGG 
AGTAATCAAG AAGGAAGAGC AAATGTAAAC ATGAAAAGGG AGACGGGTGA GCAGACAAAG 
GACCTAGAGA AGAGTGAGGC AAAGAAGAAA GAGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005635516.1
CDS	189..2207
Translation	MEQEPQNGEPAEIKIIKEAYKKAFLFVNKGLNTDELGQKEEAKSYYKQGIGHLLRGISISSTEPEHIGPGWESARQMQQKMKETLQNVRTRLEILEKGLATSLRNDLQEVPKLYPEFPPKDMSEKSPEPQSFSSPHQHAEVNGNTSTTSTGLVPAPSSLSIPSQSHPAEAPPAYTPQAAEGHYTVSYGTESGEFSSVGEDFYRNHSQPPPLETLGLDADELILIPNGVQIFFVNPAGEVSAPSYPGYLRIVRFLDNSLDTVLNRPPGFLQVCDWLYPLVPDRSPVLKCTVGAYMFPDTMLQASGCFVGVVLSSELPEDDRELFEDLLRQMSDLRLQANWNRAEGENEFQIPGRARCTSDQLKEASGTDVRHLGPSLDQDNKDVRHKGKRGKKAKDTSSEEVNLSHIVPYEPVSEEKAKELPEWSEKVAQNILSGASWVSWGLVKGAEFTGKAIQKGASKLRERIQPEEKPVEVSPAVTRGLYIAKQATGGAAKVSQFLVDGVCTVANCVGKELAPHVKKHGSKLVPESLKKDRNGKSTLDGAMVVAASSVQGFSTVWQGLECAAKCIVNNVSAETVQTVRYKYGHTAGEATHDAVDSAINVGVTAYNIDNIGIKAMVKKTAKETGQTLLEDYKIVDNSKGSNQEGRANVNMKRETGEQTKDLEKSEAKKKEK

Target ORF information:

RefSeq Version	XM_005635459.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris spastic paraplegia 20 (Troyer syndrome) (SPG20), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005635459.2

ORF Insert Sequence:

ATGGAGCAAG AGCCACAAAA TGGAGAACCT GCTGAAATTA AGATCATCAA GGAAGCATAC 
AAGAAAGCCT TTTTATTTGT TAATAAAGGA CTGAATACAG ATGAATTAGG TCAGAAGGAA 
GAAGCAAAGA GCTACTATAA GCAAGGAATA GGACATCTGC TCAGAGGAAT CAGCATATCA 
TCAACAGAGC CTGAACACAT AGGTCCTGGG TGGGAATCTG CTAGACAGAT GCAACAGAAA 
ATGAAAGAAA CACTACAGAA TGTACGTACC AGGTTGGAAA TTCTGGAGAA GGGTCTTGCC 
ACTTCTCTAC GGAATGATCT TCAGGAGGTG CCCAAGTTAT ATCCAGAATT TCCACCTAAA 
GACATGTCTG AAAAGTCACC AGAGCCTCAG TCCTTTAGTT CACCTCACCA GCATGCTGAA 
GTAAATGGAA ACACTTCAAC GACAAGCACA GGGTTGGTTC CTGCACCTAG TTCTTTATCC 
ATACCATCTC AAAGTCATCC AGCAGAAGCA CCTCCTGCTT ATACACCTCA GGCTGCTGAG 
GGCCACTACA CTGTATCCTA TGGAACAGAA TCTGGGGAAT TTTCGTCAGT TGGAGAAGAC 
TTTTACAGGA ATCATTCCCA GCCACCTCCT CTTGAAACCT TAGGGCTAGA TGCAGATGAG 
TTGATTTTGA TACCAAATGG AGTACAGATT TTTTTTGTAA ATCCTGCAGG GGAGGTTAGT 
GCACCTTCAT ATCCAGGGTA CCTTCGAATT GTGAGGTTCT TGGATAATTC TCTTGATACT 
GTTCTAAACC GTCCTCCTGG GTTTCTTCAG GTTTGTGACT GGTTATATCC TCTAGTTCCT 
GATAGATCTC CAGTTCTTAA ATGTACTGTG GGGGCCTACA TGTTTCCTGA TACCATGTTA 
CAAGCATCAG GATGTTTTGT GGGGGTCGTC TTGTCTTCTG AATTACCAGA AGATGATAGA 
GAACTCTTTG AGGATTTACT AAGACAGATG TCTGACCTTC GGCTTCAGGC CAACTGGAAC 
CGGGCAGAAG GAGAAAATGA ATTCCAAATT CCTGGAAGAG CAAGATGCAC CTCTGACCAA 
TTGAAGGAAG CCAGTGGCAC TGATGTGAGA CATTTGGGTC CTTCATTGGA CCAAGACAAT 
AAGGATGTGC GTCATAAAGG AAAACGGGGG AAAAAAGCCA AAGACACTTC AAGTGAAGAA 
GTTAATCTGA GTCACATTGT ACCCTATGAG CCAGTTTCAG AAGAAAAAGC AAAGGAATTA 
CCTGAATGGA GTGAGAAAGT GGCTCAAAAT ATTTTGTCAG GTGCTTCCTG GGTGAGCTGG 
GGTTTGGTCA AAGGTGCTGA ATTTACTGGC AAAGCAATCC AGAAAGGCGC TTCTAAACTC 
CGTGAACGGA TTCAACCAGA AGAAAAACCA GTGGAAGTTA GTCCAGCTGT GACCAGGGGA 
CTTTATATAG CAAAGCAGGC TACAGGAGGA GCAGCGAAAG TCAGTCAGTT CCTGGTTGAT 
GGAGTTTGCA CTGTAGCAAA TTGTGTTGGA AAGGAACTAG CTCCACATGT CAAGAAGCAT 
GGAAGCAAAC TTGTTCCAGA ATCTCTTAAA AAAGACAGAA ATGGAAAGTC TACCTTGGAT 
GGTGCTATGG TTGTAGCAGC AAGTAGTGTT CAAGGATTTT CAACTGTCTG GCAGGGATTG 
GAATGTGCAG CCAAATGTAT TGTTAATAAT GTTTCAGCAG AAACTGTACA AACTGTCAGA 
TACAAATACG GGCATACTGC AGGAGAAGCT ACACATGATG CAGTAGATTC TGCCATCAAT 
GTTGGTGTAA CTGCCTATAA TATTGATAAC ATTGGTATCA AAGCAATGGT GAAGAAAACT 
GCAAAAGAAA CAGGACAGAC ACTCCTGGAA GACTATAAAA TAGTTGATAA TTCTAAGGGG 
AGTAATCAAG AAGGAAGAGC AAATGTAAAC ATGAAAAGGG AGACGGGTGA GCAGACAAAG 
GACCTAGAGA AGAGTGAGGC AAAGAAGAAA GAGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPART ( XM_014107428.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013962903.1 Canis lupus familiaris spastic paraplegia 20 (Troyer syndrome) (SPG20), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OCe25372
Clone ID Related Accession (Same CDS sequence)	XM_005635459.3 , XM_005635459.2 , XM_014107428.1 , XM_014107427.1 , XM_022409585.1 , XM_014107426.1 , XM_014107426.2
Accession Version	XM_014107428.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2019bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	spartin
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726102.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCAAG AGCCACAAAA TGGAGAACCT GCTGAAATTA AGATCATCAA GGAAGCATAC 
AAGAAAGCCT TTTTATTTGT TAATAAAGGA CTGAATACAG ATGAATTAGG TCAGAAGGAA 
GAAGCAAAGA GCTACTATAA GCAAGGAATA GGACATCTGC TCAGAGGAAT CAGCATATCA 
TCAACAGAGC CTGAACACAT AGGTCCTGGG TGGGAATCTG CTAGACAGAT GCAACAGAAA 
ATGAAAGAAA CACTACAGAA TGTACGTACC AGGTTGGAAA TTCTGGAGAA GGGTCTTGCC 
ACTTCTCTAC GGAATGATCT TCAGGAGGTG CCCAAGTTAT ATCCAGAATT TCCACCTAAA 
GACATGTCTG AAAAGTCACC AGAGCCTCAG TCCTTTAGTT CACCTCACCA GCATGCTGAA 
GTAAATGGAA ACACTTCAAC GACAAGCACA GGGTTGGTTC CTGCACCTAG TTCTTTATCC 
ATACCATCTC AAAGTCATCC AGCAGAAGCA CCTCCTGCTT ATACACCTCA GGCTGCTGAG 
GGCCACTACA CTGTATCCTA TGGAACAGAA TCTGGGGAAT TTTCGTCAGT TGGAGAAGAC 
TTTTACAGGA ATCATTCCCA GCCACCTCCT CTTGAAACCT TAGGGCTAGA TGCAGATGAG 
TTGATTTTGA TACCAAATGG AGTACAGATT TTTTTTGTAA ATCCTGCAGG GGAGGTTAGT 
GCACCTTCAT ATCCAGGGTA CCTTCGAATT GTGAGGTTCT TGGATAATTC TCTTGATACT 
GTTCTAAACC GTCCTCCTGG GTTTCTTCAG GTTTGTGACT GGTTATATCC TCTAGTTCCT 
GATAGATCTC CAGTTCTTAA ATGTACTGTG GGGGCCTACA TGTTTCCTGA TACCATGTTA 
CAAGCATCAG GATGTTTTGT GGGGGTCGTC TTGTCTTCTG AATTACCAGA AGATGATAGA 
GAACTCTTTG AGGATTTACT AAGACAGATG TCTGACCTTC GGCTTCAGGC CAACTGGAAC 
CGGGCAGAAG GAGAAAATGA ATTCCAAATT CCTGGAAGAG CAAGATGCAC CTCTGACCAA 
TTGAAGGAAG CCAGTGGCAC TGATGTGAGA CATTTGGGTC CTTCATTGGA CCAAGACAAT 
AAGGATGTGC GTCATAAAGG AAAACGGGGG AAAAAAGCCA AAGACACTTC AAGTGAAGAA 
GTTAATCTGA GTCACATTGT ACCCTATGAG CCAGTTTCAG AAGAAAAAGC AAAGGAATTA 
CCTGAATGGA GTGAGAAAGT GGCTCAAAAT ATTTTGTCAG GTGCTTCCTG GGTGAGCTGG 
GGTTTGGTCA AAGGTGCTGA ATTTACTGGC AAAGCAATCC AGAAAGGCGC TTCTAAACTC 
CGTGAACGGA TTCAACCAGA AGAAAAACCA GTGGAAGTTA GTCCAGCTGT GACCAGGGGA 
CTTTATATAG CAAAGCAGGC TACAGGAGGA GCAGCGAAAG TCAGTCAGTT CCTGGTTGAT 
GGAGTTTGCA CTGTAGCAAA TTGTGTTGGA AAGGAACTAG CTCCACATGT CAAGAAGCAT 
GGAAGCAAAC TTGTTCCAGA ATCTCTTAAA AAAGACAGAA ATGGAAAGTC TACCTTGGAT 
GGTGCTATGG TTGTAGCAGC AAGTAGTGTT CAAGGATTTT CAACTGTCTG GCAGGGATTG 
GAATGTGCAG CCAAATGTAT TGTTAATAAT GTTTCAGCAG AAACTGTACA AACTGTCAGA 
TACAAATACG GGCATACTGC AGGAGAAGCT ACACATGATG CAGTAGATTC TGCCATCAAT 
GTTGGTGTAA CTGCCTATAA TATTGATAAC ATTGGTATCA AAGCAATGGT GAAGAAAACT 
GCAAAAGAAA CAGGACAGAC ACTCCTGGAA GACTATAAAA TAGTTGATAA TTCTAAGGGG 
AGTAATCAAG AAGGAAGAGC AAATGTAAAC ATGAAAAGGG AGACGGGTGA GCAGACAAAG 
GACCTAGAGA AGAGTGAGGC AAAGAAGAAA GAGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013962903.1
CDS	26..2044
Translation	MEQEPQNGEPAEIKIIKEAYKKAFLFVNKGLNTDELGQKEEAKSYYKQGIGHLLRGISISSTEPEHIGPGWESARQMQQKMKETLQNVRTRLEILEKGLATSLRNDLQEVPKLYPEFPPKDMSEKSPEPQSFSSPHQHAEVNGNTSTTSTGLVPAPSSLSIPSQSHPAEAPPAYTPQAAEGHYTVSYGTESGEFSSVGEDFYRNHSQPPPLETLGLDADELILIPNGVQIFFVNPAGEVSAPSYPGYLRIVRFLDNSLDTVLNRPPGFLQVCDWLYPLVPDRSPVLKCTVGAYMFPDTMLQASGCFVGVVLSSELPEDDRELFEDLLRQMSDLRLQANWNRAEGENEFQIPGRARCTSDQLKEASGTDVRHLGPSLDQDNKDVRHKGKRGKKAKDTSSEEVNLSHIVPYEPVSEEKAKELPEWSEKVAQNILSGASWVSWGLVKGAEFTGKAIQKGASKLRERIQPEEKPVEVSPAVTRGLYIAKQATGGAAKVSQFLVDGVCTVANCVGKELAPHVKKHGSKLVPESLKKDRNGKSTLDGAMVVAASSVQGFSTVWQGLECAAKCIVNNVSAETVQTVRYKYGHTAGEATHDAVDSAINVGVTAYNIDNIGIKAMVKKTAKETGQTLLEDYKIVDNSKGSNQEGRANVNMKRETGEQTKDLEKSEAKKKEK

Target ORF information:

RefSeq Version	XM_014107428.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris spastic paraplegia 20 (Troyer syndrome) (SPG20), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014107428.1

ORF Insert Sequence:

ATGGAGCAAG AGCCACAAAA TGGAGAACCT GCTGAAATTA AGATCATCAA GGAAGCATAC 
AAGAAAGCCT TTTTATTTGT TAATAAAGGA CTGAATACAG ATGAATTAGG TCAGAAGGAA 
GAAGCAAAGA GCTACTATAA GCAAGGAATA GGACATCTGC TCAGAGGAAT CAGCATATCA 
TCAACAGAGC CTGAACACAT AGGTCCTGGG TGGGAATCTG CTAGACAGAT GCAACAGAAA 
ATGAAAGAAA CACTACAGAA TGTACGTACC AGGTTGGAAA TTCTGGAGAA GGGTCTTGCC 
ACTTCTCTAC GGAATGATCT TCAGGAGGTG CCCAAGTTAT ATCCAGAATT TCCACCTAAA 
GACATGTCTG AAAAGTCACC AGAGCCTCAG TCCTTTAGTT CACCTCACCA GCATGCTGAA 
GTAAATGGAA ACACTTCAAC GACAAGCACA GGGTTGGTTC CTGCACCTAG TTCTTTATCC 
ATACCATCTC AAAGTCATCC AGCAGAAGCA CCTCCTGCTT ATACACCTCA GGCTGCTGAG 
GGCCACTACA CTGTATCCTA TGGAACAGAA TCTGGGGAAT TTTCGTCAGT TGGAGAAGAC 
TTTTACAGGA ATCATTCCCA GCCACCTCCT CTTGAAACCT TAGGGCTAGA TGCAGATGAG 
TTGATTTTGA TACCAAATGG AGTACAGATT TTTTTTGTAA ATCCTGCAGG GGAGGTTAGT 
GCACCTTCAT ATCCAGGGTA CCTTCGAATT GTGAGGTTCT TGGATAATTC TCTTGATACT 
GTTCTAAACC GTCCTCCTGG GTTTCTTCAG GTTTGTGACT GGTTATATCC TCTAGTTCCT 
GATAGATCTC CAGTTCTTAA ATGTACTGTG GGGGCCTACA TGTTTCCTGA TACCATGTTA 
CAAGCATCAG GATGTTTTGT GGGGGTCGTC TTGTCTTCTG AATTACCAGA AGATGATAGA 
GAACTCTTTG AGGATTTACT AAGACAGATG TCTGACCTTC GGCTTCAGGC CAACTGGAAC 
CGGGCAGAAG GAGAAAATGA ATTCCAAATT CCTGGAAGAG CAAGATGCAC CTCTGACCAA 
TTGAAGGAAG CCAGTGGCAC TGATGTGAGA CATTTGGGTC CTTCATTGGA CCAAGACAAT 
AAGGATGTGC GTCATAAAGG AAAACGGGGG AAAAAAGCCA AAGACACTTC AAGTGAAGAA 
GTTAATCTGA GTCACATTGT ACCCTATGAG CCAGTTTCAG AAGAAAAAGC AAAGGAATTA 
CCTGAATGGA GTGAGAAAGT GGCTCAAAAT ATTTTGTCAG GTGCTTCCTG GGTGAGCTGG 
GGTTTGGTCA AAGGTGCTGA ATTTACTGGC AAAGCAATCC AGAAAGGCGC TTCTAAACTC 
CGTGAACGGA TTCAACCAGA AGAAAAACCA GTGGAAGTTA GTCCAGCTGT GACCAGGGGA 
CTTTATATAG CAAAGCAGGC TACAGGAGGA GCAGCGAAAG TCAGTCAGTT CCTGGTTGAT 
GGAGTTTGCA CTGTAGCAAA TTGTGTTGGA AAGGAACTAG CTCCACATGT CAAGAAGCAT 
GGAAGCAAAC TTGTTCCAGA ATCTCTTAAA AAAGACAGAA ATGGAAAGTC TACCTTGGAT 
GGTGCTATGG TTGTAGCAGC AAGTAGTGTT CAAGGATTTT CAACTGTCTG GCAGGGATTG 
GAATGTGCAG CCAAATGTAT TGTTAATAAT GTTTCAGCAG AAACTGTACA AACTGTCAGA 
TACAAATACG GGCATACTGC AGGAGAAGCT ACACATGATG CAGTAGATTC TGCCATCAAT 
GTTGGTGTAA CTGCCTATAA TATTGATAAC ATTGGTATCA AAGCAATGGT GAAGAAAACT 
GCAAAAGAAA CAGGACAGAC ACTCCTGGAA GACTATAAAA TAGTTGATAA TTCTAAGGGG 
AGTAATCAAG AAGGAAGAGC AAATGTAAAC ATGAAAAGGG AGACGGGTGA GCAGACAAAG 
GACCTAGAGA AGAGTGAGGC AAAGAAGAAA GAGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPART ( XM_014107427.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013962902.1 Canis lupus familiaris spastic paraplegia 20 (Troyer syndrome) (SPG20), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OCe25372
Clone ID Related Accession (Same CDS sequence)	XM_005635459.3 , XM_005635459.2 , XM_014107428.1 , XM_014107427.1 , XM_022409585.1 , XM_014107426.1 , XM_014107426.2
Accession Version	XM_014107427.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2019bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	spartin
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726102.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCAAG AGCCACAAAA TGGAGAACCT GCTGAAATTA AGATCATCAA GGAAGCATAC 
AAGAAAGCCT TTTTATTTGT TAATAAAGGA CTGAATACAG ATGAATTAGG TCAGAAGGAA 
GAAGCAAAGA GCTACTATAA GCAAGGAATA GGACATCTGC TCAGAGGAAT CAGCATATCA 
TCAACAGAGC CTGAACACAT AGGTCCTGGG TGGGAATCTG CTAGACAGAT GCAACAGAAA 
ATGAAAGAAA CACTACAGAA TGTACGTACC AGGTTGGAAA TTCTGGAGAA GGGTCTTGCC 
ACTTCTCTAC GGAATGATCT TCAGGAGGTG CCCAAGTTAT ATCCAGAATT TCCACCTAAA 
GACATGTCTG AAAAGTCACC AGAGCCTCAG TCCTTTAGTT CACCTCACCA GCATGCTGAA 
GTAAATGGAA ACACTTCAAC GACAAGCACA GGGTTGGTTC CTGCACCTAG TTCTTTATCC 
ATACCATCTC AAAGTCATCC AGCAGAAGCA CCTCCTGCTT ATACACCTCA GGCTGCTGAG 
GGCCACTACA CTGTATCCTA TGGAACAGAA TCTGGGGAAT TTTCGTCAGT TGGAGAAGAC 
TTTTACAGGA ATCATTCCCA GCCACCTCCT CTTGAAACCT TAGGGCTAGA TGCAGATGAG 
TTGATTTTGA TACCAAATGG AGTACAGATT TTTTTTGTAA ATCCTGCAGG GGAGGTTAGT 
GCACCTTCAT ATCCAGGGTA CCTTCGAATT GTGAGGTTCT TGGATAATTC TCTTGATACT 
GTTCTAAACC GTCCTCCTGG GTTTCTTCAG GTTTGTGACT GGTTATATCC TCTAGTTCCT 
GATAGATCTC CAGTTCTTAA ATGTACTGTG GGGGCCTACA TGTTTCCTGA TACCATGTTA 
CAAGCATCAG GATGTTTTGT GGGGGTCGTC TTGTCTTCTG AATTACCAGA AGATGATAGA 
GAACTCTTTG AGGATTTACT AAGACAGATG TCTGACCTTC GGCTTCAGGC CAACTGGAAC 
CGGGCAGAAG GAGAAAATGA ATTCCAAATT CCTGGAAGAG CAAGATGCAC CTCTGACCAA 
TTGAAGGAAG CCAGTGGCAC TGATGTGAGA CATTTGGGTC CTTCATTGGA CCAAGACAAT 
AAGGATGTGC GTCATAAAGG AAAACGGGGG AAAAAAGCCA AAGACACTTC AAGTGAAGAA 
GTTAATCTGA GTCACATTGT ACCCTATGAG CCAGTTTCAG AAGAAAAAGC AAAGGAATTA 
CCTGAATGGA GTGAGAAAGT GGCTCAAAAT ATTTTGTCAG GTGCTTCCTG GGTGAGCTGG 
GGTTTGGTCA AAGGTGCTGA ATTTACTGGC AAAGCAATCC AGAAAGGCGC TTCTAAACTC 
CGTGAACGGA TTCAACCAGA AGAAAAACCA GTGGAAGTTA GTCCAGCTGT GACCAGGGGA 
CTTTATATAG CAAAGCAGGC TACAGGAGGA GCAGCGAAAG TCAGTCAGTT CCTGGTTGAT 
GGAGTTTGCA CTGTAGCAAA TTGTGTTGGA AAGGAACTAG CTCCACATGT CAAGAAGCAT 
GGAAGCAAAC TTGTTCCAGA ATCTCTTAAA AAAGACAGAA ATGGAAAGTC TACCTTGGAT 
GGTGCTATGG TTGTAGCAGC AAGTAGTGTT CAAGGATTTT CAACTGTCTG GCAGGGATTG 
GAATGTGCAG CCAAATGTAT TGTTAATAAT GTTTCAGCAG AAACTGTACA AACTGTCAGA 
TACAAATACG GGCATACTGC AGGAGAAGCT ACACATGATG CAGTAGATTC TGCCATCAAT 
GTTGGTGTAA CTGCCTATAA TATTGATAAC ATTGGTATCA AAGCAATGGT GAAGAAAACT 
GCAAAAGAAA CAGGACAGAC ACTCCTGGAA GACTATAAAA TAGTTGATAA TTCTAAGGGG 
AGTAATCAAG AAGGAAGAGC AAATGTAAAC ATGAAAAGGG AGACGGGTGA GCAGACAAAG 
GACCTAGAGA AGAGTGAGGC AAAGAAGAAA GAGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013962902.1
CDS	512..2530
Translation	MEQEPQNGEPAEIKIIKEAYKKAFLFVNKGLNTDELGQKEEAKSYYKQGIGHLLRGISISSTEPEHIGPGWESARQMQQKMKETLQNVRTRLEILEKGLATSLRNDLQEVPKLYPEFPPKDMSEKSPEPQSFSSPHQHAEVNGNTSTTSTGLVPAPSSLSIPSQSHPAEAPPAYTPQAAEGHYTVSYGTESGEFSSVGEDFYRNHSQPPPLETLGLDADELILIPNGVQIFFVNPAGEVSAPSYPGYLRIVRFLDNSLDTVLNRPPGFLQVCDWLYPLVPDRSPVLKCTVGAYMFPDTMLQASGCFVGVVLSSELPEDDRELFEDLLRQMSDLRLQANWNRAEGENEFQIPGRARCTSDQLKEASGTDVRHLGPSLDQDNKDVRHKGKRGKKAKDTSSEEVNLSHIVPYEPVSEEKAKELPEWSEKVAQNILSGASWVSWGLVKGAEFTGKAIQKGASKLRERIQPEEKPVEVSPAVTRGLYIAKQATGGAAKVSQFLVDGVCTVANCVGKELAPHVKKHGSKLVPESLKKDRNGKSTLDGAMVVAASSVQGFSTVWQGLECAAKCIVNNVSAETVQTVRYKYGHTAGEATHDAVDSAINVGVTAYNIDNIGIKAMVKKTAKETGQTLLEDYKIVDNSKGSNQEGRANVNMKRETGEQTKDLEKSEAKKKEK

Target ORF information:

RefSeq Version	XM_014107427.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris spastic paraplegia 20 (Troyer syndrome) (SPG20), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014107427.1

ORF Insert Sequence:

ATGGAGCAAG AGCCACAAAA TGGAGAACCT GCTGAAATTA AGATCATCAA GGAAGCATAC 
AAGAAAGCCT TTTTATTTGT TAATAAAGGA CTGAATACAG ATGAATTAGG TCAGAAGGAA 
GAAGCAAAGA GCTACTATAA GCAAGGAATA GGACATCTGC TCAGAGGAAT CAGCATATCA 
TCAACAGAGC CTGAACACAT AGGTCCTGGG TGGGAATCTG CTAGACAGAT GCAACAGAAA 
ATGAAAGAAA CACTACAGAA TGTACGTACC AGGTTGGAAA TTCTGGAGAA GGGTCTTGCC 
ACTTCTCTAC GGAATGATCT TCAGGAGGTG CCCAAGTTAT ATCCAGAATT TCCACCTAAA 
GACATGTCTG AAAAGTCACC AGAGCCTCAG TCCTTTAGTT CACCTCACCA GCATGCTGAA 
GTAAATGGAA ACACTTCAAC GACAAGCACA GGGTTGGTTC CTGCACCTAG TTCTTTATCC 
ATACCATCTC AAAGTCATCC AGCAGAAGCA CCTCCTGCTT ATACACCTCA GGCTGCTGAG 
GGCCACTACA CTGTATCCTA TGGAACAGAA TCTGGGGAAT TTTCGTCAGT TGGAGAAGAC 
TTTTACAGGA ATCATTCCCA GCCACCTCCT CTTGAAACCT TAGGGCTAGA TGCAGATGAG 
TTGATTTTGA TACCAAATGG AGTACAGATT TTTTTTGTAA ATCCTGCAGG GGAGGTTAGT 
GCACCTTCAT ATCCAGGGTA CCTTCGAATT GTGAGGTTCT TGGATAATTC TCTTGATACT 
GTTCTAAACC GTCCTCCTGG GTTTCTTCAG GTTTGTGACT GGTTATATCC TCTAGTTCCT 
GATAGATCTC CAGTTCTTAA ATGTACTGTG GGGGCCTACA TGTTTCCTGA TACCATGTTA 
CAAGCATCAG GATGTTTTGT GGGGGTCGTC TTGTCTTCTG AATTACCAGA AGATGATAGA 
GAACTCTTTG AGGATTTACT AAGACAGATG TCTGACCTTC GGCTTCAGGC CAACTGGAAC 
CGGGCAGAAG GAGAAAATGA ATTCCAAATT CCTGGAAGAG CAAGATGCAC CTCTGACCAA 
TTGAAGGAAG CCAGTGGCAC TGATGTGAGA CATTTGGGTC CTTCATTGGA CCAAGACAAT 
AAGGATGTGC GTCATAAAGG AAAACGGGGG AAAAAAGCCA AAGACACTTC AAGTGAAGAA 
GTTAATCTGA GTCACATTGT ACCCTATGAG CCAGTTTCAG AAGAAAAAGC AAAGGAATTA 
CCTGAATGGA GTGAGAAAGT GGCTCAAAAT ATTTTGTCAG GTGCTTCCTG GGTGAGCTGG 
GGTTTGGTCA AAGGTGCTGA ATTTACTGGC AAAGCAATCC AGAAAGGCGC TTCTAAACTC 
CGTGAACGGA TTCAACCAGA AGAAAAACCA GTGGAAGTTA GTCCAGCTGT GACCAGGGGA 
CTTTATATAG CAAAGCAGGC TACAGGAGGA GCAGCGAAAG TCAGTCAGTT CCTGGTTGAT 
GGAGTTTGCA CTGTAGCAAA TTGTGTTGGA AAGGAACTAG CTCCACATGT CAAGAAGCAT 
GGAAGCAAAC TTGTTCCAGA ATCTCTTAAA AAAGACAGAA ATGGAAAGTC TACCTTGGAT 
GGTGCTATGG TTGTAGCAGC AAGTAGTGTT CAAGGATTTT CAACTGTCTG GCAGGGATTG 
GAATGTGCAG CCAAATGTAT TGTTAATAAT GTTTCAGCAG AAACTGTACA AACTGTCAGA 
TACAAATACG GGCATACTGC AGGAGAAGCT ACACATGATG CAGTAGATTC TGCCATCAAT 
GTTGGTGTAA CTGCCTATAA TATTGATAAC ATTGGTATCA AAGCAATGGT GAAGAAAACT 
GCAAAAGAAA CAGGACAGAC ACTCCTGGAA GACTATAAAA TAGTTGATAA TTCTAAGGGG 
AGTAATCAAG AAGGAAGAGC AAATGTAAAC ATGAAAAGGG AGACGGGTGA GCAGACAAAG 
GACCTAGAGA AGAGTGAGGC AAAGAAGAAA GAGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPART ( XM_022409585.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022265293.1 Canis lupus familiaris spartin (SPART), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OCe25372
Clone ID Related Accession (Same CDS sequence)	XM_005635459.3 , XM_005635459.2 , XM_014107428.1 , XM_014107427.1 , XM_022409585.1 , XM_014107426.1 , XM_014107426.2
Accession Version	XM_022409585.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2019bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	spartin
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006607.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCAAG AGCCACAAAA TGGAGAACCT GCTGAAATTA AGATCATCAA GGAAGCATAC 
AAGAAAGCCT TTTTATTTGT TAATAAAGGA CTGAATACAG ATGAATTAGG TCAGAAGGAA 
GAAGCAAAGA GCTACTATAA GCAAGGAATA GGACATCTGC TCAGAGGAAT CAGCATATCA 
TCAACAGAGC CTGAACACAT AGGTCCTGGG TGGGAATCTG CTAGACAGAT GCAACAGAAA 
ATGAAAGAAA CACTACAGAA TGTACGTACC AGGTTGGAAA TTCTGGAGAA GGGTCTTGCC 
ACTTCTCTAC GGAATGATCT TCAGGAGGTG CCCAAGTTAT ATCCAGAATT TCCACCTAAA 
GACATGTCTG AAAAGTCACC AGAGCCTCAG TCCTTTAGTT CACCTCACCA GCATGCTGAA 
GTAAATGGAA ACACTTCAAC GACAAGCACA GGGTTGGTTC CTGCACCTAG TTCTTTATCC 
ATACCATCTC AAAGTCATCC AGCAGAAGCA CCTCCTGCTT ATACACCTCA GGCTGCTGAG 
GGCCACTACA CTGTATCCTA TGGAACAGAA TCTGGGGAAT TTTCGTCAGT TGGAGAAGAC 
TTTTACAGGA ATCATTCCCA GCCACCTCCT CTTGAAACCT TAGGGCTAGA TGCAGATGAG 
TTGATTTTGA TACCAAATGG AGTACAGATT TTTTTTGTAA ATCCTGCAGG GGAGGTTAGT 
GCACCTTCAT ATCCAGGGTA CCTTCGAATT GTGAGGTTCT TGGATAATTC TCTTGATACT 
GTTCTAAACC GTCCTCCTGG GTTTCTTCAG GTTTGTGACT GGTTATATCC TCTAGTTCCT 
GATAGATCTC CAGTTCTTAA ATGTACTGTG GGGGCCTACA TGTTTCCTGA TACCATGTTA 
CAAGCATCAG GATGTTTTGT GGGGGTCGTC TTGTCTTCTG AATTACCAGA AGATGATAGA 
GAACTCTTTG AGGATTTACT AAGACAGATG TCTGACCTTC GGCTTCAGGC CAACTGGAAC 
CGGGCAGAAG GAGAAAATGA ATTCCAAATT CCTGGAAGAG CAAGATGCAC CTCTGACCAA 
TTGAAGGAAG CCAGTGGCAC TGATGTGAGA CATTTGGGTC CTTCATTGGA CCAAGACAAT 
AAGGATGTGC GTCATAAAGG AAAACGGGGG AAAAAAGCCA AAGACACTTC AAGTGAAGAA 
GTTAATCTGA GTCACATTGT ACCCTATGAG CCAGTTTCAG AAGAAAAAGC AAAGGAATTA 
CCTGAATGGA GTGAGAAAGT GGCTCAAAAT ATTTTGTCAG GTGCTTCCTG GGTGAGCTGG 
GGTTTGGTCA AAGGTGCTGA ATTTACTGGC AAAGCAATCC AGAAAGGCGC TTCTAAACTC 
CGTGAACGGA TTCAACCAGA AGAAAAACCA GTGGAAGTTA GTCCAGCTGT GACCAGGGGA 
CTTTATATAG CAAAGCAGGC TACAGGAGGA GCAGCGAAAG TCAGTCAGTT CCTGGTTGAT 
GGAGTTTGCA CTGTAGCAAA TTGTGTTGGA AAGGAACTAG CTCCACATGT CAAGAAGCAT 
GGAAGCAAAC TTGTTCCAGA ATCTCTTAAA AAAGACAGAA ATGGAAAGTC TACCTTGGAT 
GGTGCTATGG TTGTAGCAGC AAGTAGTGTT CAAGGATTTT CAACTGTCTG GCAGGGATTG 
GAATGTGCAG CCAAATGTAT TGTTAATAAT GTTTCAGCAG AAACTGTACA AACTGTCAGA 
TACAAATACG GGCATACTGC AGGAGAAGCT ACACATGATG CAGTAGATTC TGCCATCAAT 
GTTGGTGTAA CTGCCTATAA TATTGATAAC ATTGGTATCA AAGCAATGGT GAAGAAAACT 
GCAAAAGAAA CAGGACAGAC ACTCCTGGAA GACTATAAAA TAGTTGATAA TTCTAAGGGG 
AGTAATCAAG AAGGAAGAGC AAATGTAAAC ATGAAAAGGG AGACGGGTGA GCAGACAAAG 
GACCTAGAGA AGAGTGAGGC AAAGAAGAAA GAGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022265293.1
CDS	328..2346
Translation	MEQEPQNGEPAEIKIIKEAYKKAFLFVNKGLNTDELGQKEEAKSYYKQGIGHLLRGISISSTEPEHIGPGWESARQMQQKMKETLQNVRTRLEILEKGLATSLRNDLQEVPKLYPEFPPKDMSEKSPEPQSFSSPHQHAEVNGNTSTTSTGLVPAPSSLSIPSQSHPAEAPPAYTPQAAEGHYTVSYGTESGEFSSVGEDFYRNHSQPPPLETLGLDADELILIPNGVQIFFVNPAGEVSAPSYPGYLRIVRFLDNSLDTVLNRPPGFLQVCDWLYPLVPDRSPVLKCTVGAYMFPDTMLQASGCFVGVVLSSELPEDDRELFEDLLRQMSDLRLQANWNRAEGENEFQIPGRARCTSDQLKEASGTDVRHLGPSLDQDNKDVRHKGKRGKKAKDTSSEEVNLSHIVPYEPVSEEKAKELPEWSEKVAQNILSGASWVSWGLVKGAEFTGKAIQKGASKLRERIQPEEKPVEVSPAVTRGLYIAKQATGGAAKVSQFLVDGVCTVANCVGKELAPHVKKHGSKLVPESLKKDRNGKSTLDGAMVVAASSVQGFSTVWQGLECAAKCIVNNVSAETVQTVRYKYGHTAGEATHDAVDSAINVGVTAYNIDNIGIKAMVKKTAKETGQTLLEDYKIVDNSKGSNQEGRANVNMKRETGEQTKDLEKSEAKKKEK

Target ORF information:

RefSeq Version	XM_022409585.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris spartin (SPART), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022409585.1

ORF Insert Sequence:

ATGGAGCAAG AGCCACAAAA TGGAGAACCT GCTGAAATTA AGATCATCAA GGAAGCATAC 
AAGAAAGCCT TTTTATTTGT TAATAAAGGA CTGAATACAG ATGAATTAGG TCAGAAGGAA 
GAAGCAAAGA GCTACTATAA GCAAGGAATA GGACATCTGC TCAGAGGAAT CAGCATATCA 
TCAACAGAGC CTGAACACAT AGGTCCTGGG TGGGAATCTG CTAGACAGAT GCAACAGAAA 
ATGAAAGAAA CACTACAGAA TGTACGTACC AGGTTGGAAA TTCTGGAGAA GGGTCTTGCC 
ACTTCTCTAC GGAATGATCT TCAGGAGGTG CCCAAGTTAT ATCCAGAATT TCCACCTAAA 
GACATGTCTG AAAAGTCACC AGAGCCTCAG TCCTTTAGTT CACCTCACCA GCATGCTGAA 
GTAAATGGAA ACACTTCAAC GACAAGCACA GGGTTGGTTC CTGCACCTAG TTCTTTATCC 
ATACCATCTC AAAGTCATCC AGCAGAAGCA CCTCCTGCTT ATACACCTCA GGCTGCTGAG 
GGCCACTACA CTGTATCCTA TGGAACAGAA TCTGGGGAAT TTTCGTCAGT TGGAGAAGAC 
TTTTACAGGA ATCATTCCCA GCCACCTCCT CTTGAAACCT TAGGGCTAGA TGCAGATGAG 
TTGATTTTGA TACCAAATGG AGTACAGATT TTTTTTGTAA ATCCTGCAGG GGAGGTTAGT 
GCACCTTCAT ATCCAGGGTA CCTTCGAATT GTGAGGTTCT TGGATAATTC TCTTGATACT 
GTTCTAAACC GTCCTCCTGG GTTTCTTCAG GTTTGTGACT GGTTATATCC TCTAGTTCCT 
GATAGATCTC CAGTTCTTAA ATGTACTGTG GGGGCCTACA TGTTTCCTGA TACCATGTTA 
CAAGCATCAG GATGTTTTGT GGGGGTCGTC TTGTCTTCTG AATTACCAGA AGATGATAGA 
GAACTCTTTG AGGATTTACT AAGACAGATG TCTGACCTTC GGCTTCAGGC CAACTGGAAC 
CGGGCAGAAG GAGAAAATGA ATTCCAAATT CCTGGAAGAG CAAGATGCAC CTCTGACCAA 
TTGAAGGAAG CCAGTGGCAC TGATGTGAGA CATTTGGGTC CTTCATTGGA CCAAGACAAT 
AAGGATGTGC GTCATAAAGG AAAACGGGGG AAAAAAGCCA AAGACACTTC AAGTGAAGAA 
GTTAATCTGA GTCACATTGT ACCCTATGAG CCAGTTTCAG AAGAAAAAGC AAAGGAATTA 
CCTGAATGGA GTGAGAAAGT GGCTCAAAAT ATTTTGTCAG GTGCTTCCTG GGTGAGCTGG 
GGTTTGGTCA AAGGTGCTGA ATTTACTGGC AAAGCAATCC AGAAAGGCGC TTCTAAACTC 
CGTGAACGGA TTCAACCAGA AGAAAAACCA GTGGAAGTTA GTCCAGCTGT GACCAGGGGA 
CTTTATATAG CAAAGCAGGC TACAGGAGGA GCAGCGAAAG TCAGTCAGTT CCTGGTTGAT 
GGAGTTTGCA CTGTAGCAAA TTGTGTTGGA AAGGAACTAG CTCCACATGT CAAGAAGCAT 
GGAAGCAAAC TTGTTCCAGA ATCTCTTAAA AAAGACAGAA ATGGAAAGTC TACCTTGGAT 
GGTGCTATGG TTGTAGCAGC AAGTAGTGTT CAAGGATTTT CAACTGTCTG GCAGGGATTG 
GAATGTGCAG CCAAATGTAT TGTTAATAAT GTTTCAGCAG AAACTGTACA AACTGTCAGA 
TACAAATACG GGCATACTGC AGGAGAAGCT ACACATGATG CAGTAGATTC TGCCATCAAT 
GTTGGTGTAA CTGCCTATAA TATTGATAAC ATTGGTATCA AAGCAATGGT GAAGAAAACT 
GCAAAAGAAA CAGGACAGAC ACTCCTGGAA GACTATAAAA TAGTTGATAA TTCTAAGGGG 
AGTAATCAAG AAGGAAGAGC AAATGTAAAC ATGAAAAGGG AGACGGGTGA GCAGACAAAG 
GACCTAGAGA AGAGTGAGGC AAAGAAGAAA GAGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPART ( XM_014107426.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013962901.1 Canis lupus familiaris spastic paraplegia 20 (Troyer syndrome) (SPG20), transcript variant X2, mRNA.

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CloneID	OCe25372
Clone ID Related Accession (Same CDS sequence)	XM_005635459.3 , XM_005635459.2 , XM_014107428.1 , XM_014107427.1 , XM_022409585.1 , XM_014107426.1 , XM_014107426.2
Accession Version	XM_014107426.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2019bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	spartin
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726102.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCAAG AGCCACAAAA TGGAGAACCT GCTGAAATTA AGATCATCAA GGAAGCATAC 
AAGAAAGCCT TTTTATTTGT TAATAAAGGA CTGAATACAG ATGAATTAGG TCAGAAGGAA 
GAAGCAAAGA GCTACTATAA GCAAGGAATA GGACATCTGC TCAGAGGAAT CAGCATATCA 
TCAACAGAGC CTGAACACAT AGGTCCTGGG TGGGAATCTG CTAGACAGAT GCAACAGAAA 
ATGAAAGAAA CACTACAGAA TGTACGTACC AGGTTGGAAA TTCTGGAGAA GGGTCTTGCC 
ACTTCTCTAC GGAATGATCT TCAGGAGGTG CCCAAGTTAT ATCCAGAATT TCCACCTAAA 
GACATGTCTG AAAAGTCACC AGAGCCTCAG TCCTTTAGTT CACCTCACCA GCATGCTGAA 
GTAAATGGAA ACACTTCAAC GACAAGCACA GGGTTGGTTC CTGCACCTAG TTCTTTATCC 
ATACCATCTC AAAGTCATCC AGCAGAAGCA CCTCCTGCTT ATACACCTCA GGCTGCTGAG 
GGCCACTACA CTGTATCCTA TGGAACAGAA TCTGGGGAAT TTTCGTCAGT TGGAGAAGAC 
TTTTACAGGA ATCATTCCCA GCCACCTCCT CTTGAAACCT TAGGGCTAGA TGCAGATGAG 
TTGATTTTGA TACCAAATGG AGTACAGATT TTTTTTGTAA ATCCTGCAGG GGAGGTTAGT 
GCACCTTCAT ATCCAGGGTA CCTTCGAATT GTGAGGTTCT TGGATAATTC TCTTGATACT 
GTTCTAAACC GTCCTCCTGG GTTTCTTCAG GTTTGTGACT GGTTATATCC TCTAGTTCCT 
GATAGATCTC CAGTTCTTAA ATGTACTGTG GGGGCCTACA TGTTTCCTGA TACCATGTTA 
CAAGCATCAG GATGTTTTGT GGGGGTCGTC TTGTCTTCTG AATTACCAGA AGATGATAGA 
GAACTCTTTG AGGATTTACT AAGACAGATG TCTGACCTTC GGCTTCAGGC CAACTGGAAC 
CGGGCAGAAG GAGAAAATGA ATTCCAAATT CCTGGAAGAG CAAGATGCAC CTCTGACCAA 
TTGAAGGAAG CCAGTGGCAC TGATGTGAGA CATTTGGGTC CTTCATTGGA CCAAGACAAT 
AAGGATGTGC GTCATAAAGG AAAACGGGGG AAAAAAGCCA AAGACACTTC AAGTGAAGAA 
GTTAATCTGA GTCACATTGT ACCCTATGAG CCAGTTTCAG AAGAAAAAGC AAAGGAATTA 
CCTGAATGGA GTGAGAAAGT GGCTCAAAAT ATTTTGTCAG GTGCTTCCTG GGTGAGCTGG 
GGTTTGGTCA AAGGTGCTGA ATTTACTGGC AAAGCAATCC AGAAAGGCGC TTCTAAACTC 
CGTGAACGGA TTCAACCAGA AGAAAAACCA GTGGAAGTTA GTCCAGCTGT GACCAGGGGA 
CTTTATATAG CAAAGCAGGC TACAGGAGGA GCAGCGAAAG TCAGTCAGTT CCTGGTTGAT 
GGAGTTTGCA CTGTAGCAAA TTGTGTTGGA AAGGAACTAG CTCCACATGT CAAGAAGCAT 
GGAAGCAAAC TTGTTCCAGA ATCTCTTAAA AAAGACAGAA ATGGAAAGTC TACCTTGGAT 
GGTGCTATGG TTGTAGCAGC AAGTAGTGTT CAAGGATTTT CAACTGTCTG GCAGGGATTG 
GAATGTGCAG CCAAATGTAT TGTTAATAAT GTTTCAGCAG AAACTGTACA AACTGTCAGA 
TACAAATACG GGCATACTGC AGGAGAAGCT ACACATGATG CAGTAGATTC TGCCATCAAT 
GTTGGTGTAA CTGCCTATAA TATTGATAAC ATTGGTATCA AAGCAATGGT GAAGAAAACT 
GCAAAAGAAA CAGGACAGAC ACTCCTGGAA GACTATAAAA TAGTTGATAA TTCTAAGGGG 
AGTAATCAAG AAGGAAGAGC AAATGTAAAC ATGAAAAGGG AGACGGGTGA GCAGACAAAG 
GACCTAGAGA AGAGTGAGGC AAAGAAGAAA GAGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013962901.1
CDS	335..2353
Translation	MEQEPQNGEPAEIKIIKEAYKKAFLFVNKGLNTDELGQKEEAKSYYKQGIGHLLRGISISSTEPEHIGPGWESARQMQQKMKETLQNVRTRLEILEKGLATSLRNDLQEVPKLYPEFPPKDMSEKSPEPQSFSSPHQHAEVNGNTSTTSTGLVPAPSSLSIPSQSHPAEAPPAYTPQAAEGHYTVSYGTESGEFSSVGEDFYRNHSQPPPLETLGLDADELILIPNGVQIFFVNPAGEVSAPSYPGYLRIVRFLDNSLDTVLNRPPGFLQVCDWLYPLVPDRSPVLKCTVGAYMFPDTMLQASGCFVGVVLSSELPEDDRELFEDLLRQMSDLRLQANWNRAEGENEFQIPGRARCTSDQLKEASGTDVRHLGPSLDQDNKDVRHKGKRGKKAKDTSSEEVNLSHIVPYEPVSEEKAKELPEWSEKVAQNILSGASWVSWGLVKGAEFTGKAIQKGASKLRERIQPEEKPVEVSPAVTRGLYIAKQATGGAAKVSQFLVDGVCTVANCVGKELAPHVKKHGSKLVPESLKKDRNGKSTLDGAMVVAASSVQGFSTVWQGLECAAKCIVNNVSAETVQTVRYKYGHTAGEATHDAVDSAINVGVTAYNIDNIGIKAMVKKTAKETGQTLLEDYKIVDNSKGSNQEGRANVNMKRETGEQTKDLEKSEAKKKEK

Target ORF information:

RefSeq Version	XM_014107426.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris spastic paraplegia 20 (Troyer syndrome) (SPG20), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014107426.1

ORF Insert Sequence:

ATGGAGCAAG AGCCACAAAA TGGAGAACCT GCTGAAATTA AGATCATCAA GGAAGCATAC 
AAGAAAGCCT TTTTATTTGT TAATAAAGGA CTGAATACAG ATGAATTAGG TCAGAAGGAA 
GAAGCAAAGA GCTACTATAA GCAAGGAATA GGACATCTGC TCAGAGGAAT CAGCATATCA 
TCAACAGAGC CTGAACACAT AGGTCCTGGG TGGGAATCTG CTAGACAGAT GCAACAGAAA 
ATGAAAGAAA CACTACAGAA TGTACGTACC AGGTTGGAAA TTCTGGAGAA GGGTCTTGCC 
ACTTCTCTAC GGAATGATCT TCAGGAGGTG CCCAAGTTAT ATCCAGAATT TCCACCTAAA 
GACATGTCTG AAAAGTCACC AGAGCCTCAG TCCTTTAGTT CACCTCACCA GCATGCTGAA 
GTAAATGGAA ACACTTCAAC GACAAGCACA GGGTTGGTTC CTGCACCTAG TTCTTTATCC 
ATACCATCTC AAAGTCATCC AGCAGAAGCA CCTCCTGCTT ATACACCTCA GGCTGCTGAG 
GGCCACTACA CTGTATCCTA TGGAACAGAA TCTGGGGAAT TTTCGTCAGT TGGAGAAGAC 
TTTTACAGGA ATCATTCCCA GCCACCTCCT CTTGAAACCT TAGGGCTAGA TGCAGATGAG 
TTGATTTTGA TACCAAATGG AGTACAGATT TTTTTTGTAA ATCCTGCAGG GGAGGTTAGT 
GCACCTTCAT ATCCAGGGTA CCTTCGAATT GTGAGGTTCT TGGATAATTC TCTTGATACT 
GTTCTAAACC GTCCTCCTGG GTTTCTTCAG GTTTGTGACT GGTTATATCC TCTAGTTCCT 
GATAGATCTC CAGTTCTTAA ATGTACTGTG GGGGCCTACA TGTTTCCTGA TACCATGTTA 
CAAGCATCAG GATGTTTTGT GGGGGTCGTC TTGTCTTCTG AATTACCAGA AGATGATAGA 
GAACTCTTTG AGGATTTACT AAGACAGATG TCTGACCTTC GGCTTCAGGC CAACTGGAAC 
CGGGCAGAAG GAGAAAATGA ATTCCAAATT CCTGGAAGAG CAAGATGCAC CTCTGACCAA 
TTGAAGGAAG CCAGTGGCAC TGATGTGAGA CATTTGGGTC CTTCATTGGA CCAAGACAAT 
AAGGATGTGC GTCATAAAGG AAAACGGGGG AAAAAAGCCA AAGACACTTC AAGTGAAGAA 
GTTAATCTGA GTCACATTGT ACCCTATGAG CCAGTTTCAG AAGAAAAAGC AAAGGAATTA 
CCTGAATGGA GTGAGAAAGT GGCTCAAAAT ATTTTGTCAG GTGCTTCCTG GGTGAGCTGG 
GGTTTGGTCA AAGGTGCTGA ATTTACTGGC AAAGCAATCC AGAAAGGCGC TTCTAAACTC 
CGTGAACGGA TTCAACCAGA AGAAAAACCA GTGGAAGTTA GTCCAGCTGT GACCAGGGGA 
CTTTATATAG CAAAGCAGGC TACAGGAGGA GCAGCGAAAG TCAGTCAGTT CCTGGTTGAT 
GGAGTTTGCA CTGTAGCAAA TTGTGTTGGA AAGGAACTAG CTCCACATGT CAAGAAGCAT 
GGAAGCAAAC TTGTTCCAGA ATCTCTTAAA AAAGACAGAA ATGGAAAGTC TACCTTGGAT 
GGTGCTATGG TTGTAGCAGC AAGTAGTGTT CAAGGATTTT CAACTGTCTG GCAGGGATTG 
GAATGTGCAG CCAAATGTAT TGTTAATAAT GTTTCAGCAG AAACTGTACA AACTGTCAGA 
TACAAATACG GGCATACTGC AGGAGAAGCT ACACATGATG CAGTAGATTC TGCCATCAAT 
GTTGGTGTAA CTGCCTATAA TATTGATAAC ATTGGTATCA AAGCAATGGT GAAGAAAACT 
GCAAAAGAAA CAGGACAGAC ACTCCTGGAA GACTATAAAA TAGTTGATAA TTCTAAGGGG 
AGTAATCAAG AAGGAAGAGC AAATGTAAAC ATGAAAAGGG AGACGGGTGA GCAGACAAAG 
GACCTAGAGA AGAGTGAGGC AAAGAAGAAA GAGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPART ( XM_014107426.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013962901.1 Canis lupus familiaris spartin (SPART), transcript variant X3, mRNA.

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CloneID	OCe25372
Clone ID Related Accession (Same CDS sequence)	XM_005635459.3 , XM_005635459.2 , XM_014107428.1 , XM_014107427.1 , XM_022409585.1 , XM_014107426.1 , XM_014107426.2
Accession Version	XM_014107426.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2019bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	spartin
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006607.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014107426.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCAAG AGCCACAAAA TGGAGAACCT GCTGAAATTA AGATCATCAA GGAAGCATAC 
AAGAAAGCCT TTTTATTTGT TAATAAAGGA CTGAATACAG ATGAATTAGG TCAGAAGGAA 
GAAGCAAAGA GCTACTATAA GCAAGGAATA GGACATCTGC TCAGAGGAAT CAGCATATCA 
TCAACAGAGC CTGAACACAT AGGTCCTGGG TGGGAATCTG CTAGACAGAT GCAACAGAAA 
ATGAAAGAAA CACTACAGAA TGTACGTACC AGGTTGGAAA TTCTGGAGAA GGGTCTTGCC 
ACTTCTCTAC GGAATGATCT TCAGGAGGTG CCCAAGTTAT ATCCAGAATT TCCACCTAAA 
GACATGTCTG AAAAGTCACC AGAGCCTCAG TCCTTTAGTT CACCTCACCA GCATGCTGAA 
GTAAATGGAA ACACTTCAAC GACAAGCACA GGGTTGGTTC CTGCACCTAG TTCTTTATCC 
ATACCATCTC AAAGTCATCC AGCAGAAGCA CCTCCTGCTT ATACACCTCA GGCTGCTGAG 
GGCCACTACA CTGTATCCTA TGGAACAGAA TCTGGGGAAT TTTCGTCAGT TGGAGAAGAC 
TTTTACAGGA ATCATTCCCA GCCACCTCCT CTTGAAACCT TAGGGCTAGA TGCAGATGAG 
TTGATTTTGA TACCAAATGG AGTACAGATT TTTTTTGTAA ATCCTGCAGG GGAGGTTAGT 
GCACCTTCAT ATCCAGGGTA CCTTCGAATT GTGAGGTTCT TGGATAATTC TCTTGATACT 
GTTCTAAACC GTCCTCCTGG GTTTCTTCAG GTTTGTGACT GGTTATATCC TCTAGTTCCT 
GATAGATCTC CAGTTCTTAA ATGTACTGTG GGGGCCTACA TGTTTCCTGA TACCATGTTA 
CAAGCATCAG GATGTTTTGT GGGGGTCGTC TTGTCTTCTG AATTACCAGA AGATGATAGA 
GAACTCTTTG AGGATTTACT AAGACAGATG TCTGACCTTC GGCTTCAGGC CAACTGGAAC 
CGGGCAGAAG GAGAAAATGA ATTCCAAATT CCTGGAAGAG CAAGATGCAC CTCTGACCAA 
TTGAAGGAAG CCAGTGGCAC TGATGTGAGA CATTTGGGTC CTTCATTGGA CCAAGACAAT 
AAGGATGTGC GTCATAAAGG AAAACGGGGG AAAAAAGCCA AAGACACTTC AAGTGAAGAA 
GTTAATCTGA GTCACATTGT ACCCTATGAG CCAGTTTCAG AAGAAAAAGC AAAGGAATTA 
CCTGAATGGA GTGAGAAAGT GGCTCAAAAT ATTTTGTCAG GTGCTTCCTG GGTGAGCTGG 
GGTTTGGTCA AAGGTGCTGA ATTTACTGGC AAAGCAATCC AGAAAGGCGC TTCTAAACTC 
CGTGAACGGA TTCAACCAGA AGAAAAACCA GTGGAAGTTA GTCCAGCTGT GACCAGGGGA 
CTTTATATAG CAAAGCAGGC TACAGGAGGA GCAGCGAAAG TCAGTCAGTT CCTGGTTGAT 
GGAGTTTGCA CTGTAGCAAA TTGTGTTGGA AAGGAACTAG CTCCACATGT CAAGAAGCAT 
GGAAGCAAAC TTGTTCCAGA ATCTCTTAAA AAAGACAGAA ATGGAAAGTC TACCTTGGAT 
GGTGCTATGG TTGTAGCAGC AAGTAGTGTT CAAGGATTTT CAACTGTCTG GCAGGGATTG 
GAATGTGCAG CCAAATGTAT TGTTAATAAT GTTTCAGCAG AAACTGTACA AACTGTCAGA 
TACAAATACG GGCATACTGC AGGAGAAGCT ACACATGATG CAGTAGATTC TGCCATCAAT 
GTTGGTGTAA CTGCCTATAA TATTGATAAC ATTGGTATCA AAGCAATGGT GAAGAAAACT 
GCAAAAGAAA CAGGACAGAC ACTCCTGGAA GACTATAAAA TAGTTGATAA TTCTAAGGGG 
AGTAATCAAG AAGGAAGAGC AAATGTAAAC ATGAAAAGGG AGACGGGTGA GCAGACAAAG 
GACCTAGAGA AGAGTGAGGC AAAGAAGAAA GAGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013962901.1
CDS	146..2164
Translation	MEQEPQNGEPAEIKIIKEAYKKAFLFVNKGLNTDELGQKEEAKSYYKQGIGHLLRGISISSTEPEHIGPGWESARQMQQKMKETLQNVRTRLEILEKGLATSLRNDLQEVPKLYPEFPPKDMSEKSPEPQSFSSPHQHAEVNGNTSTTSTGLVPAPSSLSIPSQSHPAEAPPAYTPQAAEGHYTVSYGTESGEFSSVGEDFYRNHSQPPPLETLGLDADELILIPNGVQIFFVNPAGEVSAPSYPGYLRIVRFLDNSLDTVLNRPPGFLQVCDWLYPLVPDRSPVLKCTVGAYMFPDTMLQASGCFVGVVLSSELPEDDRELFEDLLRQMSDLRLQANWNRAEGENEFQIPGRARCTSDQLKEASGTDVRHLGPSLDQDNKDVRHKGKRGKKAKDTSSEEVNLSHIVPYEPVSEEKAKELPEWSEKVAQNILSGASWVSWGLVKGAEFTGKAIQKGASKLRERIQPEEKPVEVSPAVTRGLYIAKQATGGAAKVSQFLVDGVCTVANCVGKELAPHVKKHGSKLVPESLKKDRNGKSTLDGAMVVAASSVQGFSTVWQGLECAAKCIVNNVSAETVQTVRYKYGHTAGEATHDAVDSAINVGVTAYNIDNIGIKAMVKKTAKETGQTLLEDYKIVDNSKGSNQEGRANVNMKRETGEQTKDLEKSEAKKKEK

Target ORF information:

RefSeq Version	XM_014107426.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris spartin (SPART), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014107426.2

ORF Insert Sequence:

ATGGAGCAAG AGCCACAAAA TGGAGAACCT GCTGAAATTA AGATCATCAA GGAAGCATAC 
AAGAAAGCCT TTTTATTTGT TAATAAAGGA CTGAATACAG ATGAATTAGG TCAGAAGGAA 
GAAGCAAAGA GCTACTATAA GCAAGGAATA GGACATCTGC TCAGAGGAAT CAGCATATCA 
TCAACAGAGC CTGAACACAT AGGTCCTGGG TGGGAATCTG CTAGACAGAT GCAACAGAAA 
ATGAAAGAAA CACTACAGAA TGTACGTACC AGGTTGGAAA TTCTGGAGAA GGGTCTTGCC 
ACTTCTCTAC GGAATGATCT TCAGGAGGTG CCCAAGTTAT ATCCAGAATT TCCACCTAAA 
GACATGTCTG AAAAGTCACC AGAGCCTCAG TCCTTTAGTT CACCTCACCA GCATGCTGAA 
GTAAATGGAA ACACTTCAAC GACAAGCACA GGGTTGGTTC CTGCACCTAG TTCTTTATCC 
ATACCATCTC AAAGTCATCC AGCAGAAGCA CCTCCTGCTT ATACACCTCA GGCTGCTGAG 
GGCCACTACA CTGTATCCTA TGGAACAGAA TCTGGGGAAT TTTCGTCAGT TGGAGAAGAC 
TTTTACAGGA ATCATTCCCA GCCACCTCCT CTTGAAACCT TAGGGCTAGA TGCAGATGAG 
TTGATTTTGA TACCAAATGG AGTACAGATT TTTTTTGTAA ATCCTGCAGG GGAGGTTAGT 
GCACCTTCAT ATCCAGGGTA CCTTCGAATT GTGAGGTTCT TGGATAATTC TCTTGATACT 
GTTCTAAACC GTCCTCCTGG GTTTCTTCAG GTTTGTGACT GGTTATATCC TCTAGTTCCT 
GATAGATCTC CAGTTCTTAA ATGTACTGTG GGGGCCTACA TGTTTCCTGA TACCATGTTA 
CAAGCATCAG GATGTTTTGT GGGGGTCGTC TTGTCTTCTG AATTACCAGA AGATGATAGA 
GAACTCTTTG AGGATTTACT AAGACAGATG TCTGACCTTC GGCTTCAGGC CAACTGGAAC 
CGGGCAGAAG GAGAAAATGA ATTCCAAATT CCTGGAAGAG CAAGATGCAC CTCTGACCAA 
TTGAAGGAAG CCAGTGGCAC TGATGTGAGA CATTTGGGTC CTTCATTGGA CCAAGACAAT 
AAGGATGTGC GTCATAAAGG AAAACGGGGG AAAAAAGCCA AAGACACTTC AAGTGAAGAA 
GTTAATCTGA GTCACATTGT ACCCTATGAG CCAGTTTCAG AAGAAAAAGC AAAGGAATTA 
CCTGAATGGA GTGAGAAAGT GGCTCAAAAT ATTTTGTCAG GTGCTTCCTG GGTGAGCTGG 
GGTTTGGTCA AAGGTGCTGA ATTTACTGGC AAAGCAATCC AGAAAGGCGC TTCTAAACTC 
CGTGAACGGA TTCAACCAGA AGAAAAACCA GTGGAAGTTA GTCCAGCTGT GACCAGGGGA 
CTTTATATAG CAAAGCAGGC TACAGGAGGA GCAGCGAAAG TCAGTCAGTT CCTGGTTGAT 
GGAGTTTGCA CTGTAGCAAA TTGTGTTGGA AAGGAACTAG CTCCACATGT CAAGAAGCAT 
GGAAGCAAAC TTGTTCCAGA ATCTCTTAAA AAAGACAGAA ATGGAAAGTC TACCTTGGAT 
GGTGCTATGG TTGTAGCAGC AAGTAGTGTT CAAGGATTTT CAACTGTCTG GCAGGGATTG 
GAATGTGCAG CCAAATGTAT TGTTAATAAT GTTTCAGCAG AAACTGTACA AACTGTCAGA 
TACAAATACG GGCATACTGC AGGAGAAGCT ACACATGATG CAGTAGATTC TGCCATCAAT 
GTTGGTGTAA CTGCCTATAA TATTGATAAC ATTGGTATCA AAGCAATGGT GAAGAAAACT 
GCAAAAGAAA CAGGACAGAC ACTCCTGGAA GACTATAAAA TAGTTGATAA TTCTAAGGGG 
AGTAATCAAG AAGGAAGAGC AAATGTAAAC ATGAAAAGGG AGACGGGTGA GCAGACAAAG 
GACCTAGAGA AGAGTGAGGC AAAGAAGAAA GAGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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SPART cDNA ORF clone, Canis lupus familiaris(dog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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FAX

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