APLP1 cDNA ORF clone, Canis lupus familiaris(dog)

The following APLP1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the APLP1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe02317 XM_005616688.3
Latest version!
Canis lupus familiaris amyloid beta precursor like protein 1 (APLP1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $342.30
$489.00
OCe02318 XM_533688.7
Latest version!
Canis lupus familiaris amyloid beta precursor like protein 1 (APLP1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $342.30
$489.00
OCe02319 XM_005616689.3
Latest version!
Canis lupus familiaris amyloid beta precursor like protein 1 (APLP1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $342.30
$489.00
OCe34642 XM_014115534.2
Latest version!
Canis lupus familiaris amyloid beta precursor like protein 1 (APLP1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $342.30
$489.00
View Old Accession Versions >>
OCe02319 XM_005616689.2 Canis lupus familiaris amyloid beta (A4) precursor-like protein 1 (APLP1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $342.30
$489.00
OCe34642 XM_014115534.1 Canis lupus familiaris amyloid beta (A4) precursor-like protein 1 (APLP1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $342.30
$489.00
OCe02318 XM_533688.6 Canis lupus familiaris amyloid beta (A4) precursor-like protein 1 (APLP1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $342.30
$489.00
OCe02317 XM_005616688.2 Canis lupus familiaris amyloid beta (A4) precursor-like protein 1 (APLP1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $342.30
$489.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe02317
    Clone ID Related Accession (Same CDS sequence) XM_005616688.2 , XM_005616688.3
    Accession Version XM_005616688.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1962bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product amyloid-like protein 1 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726047.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005616688.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGGGCCCA GCAGCCCCGC CGCTCGCGGT CTGGGCCGCC GCCGGGGTCC GCCGCCGCTG 
    CCGCTGTCGC TGCCGCTACT GCTGCTCCTC CTGCGCGCGC AGCTCGCCGT CGGGAGCCTG
    GCCGGTGGGA GCCCCGGCGC GGCCGAGGCC CCGGGGTCGG CCCAGGTTGC TGGACTATGC
    CGGCACCTAA CCCTTCACCG GGACCTGCGC ACCGGCCGCT GGGAACCAGA CCCACAGCGC
    TCGCGACGCT GTCTCCGGGA CCCGCAGCGC GTGCTGGAGT ACTGCAGACA GATGTACCCG
    GAGCTGCAGA TTGCACGTGT GGAACAGGCG ACGCAAGCCA TCCCCATGGA GCGCTGGTGC
    GGGGGTGCCC GGGGTGGCCG CTGTGCCCAT CCCCACCACC AGGTTGTGCC TTTCCGCTGC
    CTGCCAGGTG AATTCGTGAG CGAGGCCCTG CTGGTGCCTG AAGGTTGCCG GTTCTTGCAC
    CAGGAGCGCA TGGACCAGTG TGAGAGTTCC ACCCGGAGGC ATCAAGAGGC GCAGGAGGCC
    TGCAGTTCCC AGGGCCTCAT CCTGCATGGT TCCGGCATGC TTTTGCCCTG TGGCACAGAT
    CGGTTCCGAG GTGTGGAGTA TGTGTGCTGC CCCCCGCCAG TGACCCCCGA TCCGTCTGGG
    ACAGCAGCTG GTGACCCCTC CACCCGCTCC TGGCCCCTAG GGGGCAGAGT AGAGGGGGCT
    GAGGAGGAGG AAGAAGAGGA ATCCTTCCTA CAGCCGGTGG ATGATTACTT TGTGGAACCC
    CCACGGGCTG AAGAGGAAGA TGAGGAGGAA AGAATTCTAC CCTCAAGCTC CCATAGCCCT
    GCAGGGGTCA GCAAAGTGAC CACCACTCCA AGGCCCACAG ATGGTGTGGA TGTGTACTTC
    GGCATGCCTG GAGAAATCAG CGAGCACGAG GGGTTCCTGC GGGCCAAGAT GGACCTGGAG
    GAACGAAGGA TGCGCCAGAT TAATGAGGTG ATGCGTGAAT GGGCCATGGC AGACAACCAG
    TCCAAGAACC TGCCTAAAGC AGACAGACAG GCCCTGAACG AGCACTTCCA GTCCATTCTG
    CAGACCTTGG AGGAGCAGGT ATCTGGTGAG CGACAGCGCC TGGTGGAGAC CCATGCCACC
    CGAGTCATTG CTCTCATCAA CGACCAGCGC CGGGCTGCTC TGGAAGGTTT CCTGGCGGCG
    CTGCAGGGAG ATCCACCTCA GGCAGAGCGC GTCCTGCTGG CCCTGCGGCG TTACCTGCGA
    GCAGAGCAGA AGGAACAAAG GCACACACTG AGGCACTACC AGCACGTGGC TGCTGTGGAT
    CCTGAGAAGG CCCAGCAGAT GCGCTTCCAG GTGCAGACCC ATCTTCAAGT AATTGAAGAG
    AGGATGAACC AGAGCTTGGG GCTGCTTGAC CAGAACCCCC GCCTGGCTCA GGAGCTGCGG
    CCCCAGATCC AGGAGCTCCT CCACTCTGAG CACCTGGGTC CCAATGAATT GGAAGTCCCC
    GCCCCAGGGG GCAGCAGTGA GGACAAGAGT GGGCTGCAGC CTCTGGATTC CAAAGATGCA
    GACACCCCCG TGGCTCTTCC AAAAGGGTCC ACAGAACAAG ATGCTGCATC CTCTGGGAAA
    GAGAAGATGT CCCCCCTGGA GCAGTACGAG CGAAAGGTGA ATGTGTCTGT TCCAAGGGGT
    TTTCCTTTCC ACTCATCGGA GATTCAGAGA GATGAGCTGG CACCAGCCGG GACAGGTGTG
    TCCCGAGAGG CCGTGTCTGG TCTGCTGATC ATGGGAGCAG GTGGGGGCTC CCTGATTGTC
    CTCTCCCTGC TGCTCTTGCG CAGGAAGAAG CCCTATGGGG CTATCAGCCA TGGAGTGGTG
    GAGGTGGACC CTATGCTGAC CCTGGAGGAG CAGCAGCTGC GTGAACTGCA GCGTCACGGC
    TATGAGAACC CCACCTACCG CTTCCTGGAG GAACGACCCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005616745.1
    CDS96..2057
    Translation

    Target ORF information:

    RefSeq Version XM_005616688.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris amyloid beta (A4) precursor-like protein 1 (APLP1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005616688.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGGGCCCA GCAGCCCCGC CGCTCGCGGT CTGGGCCGCC GCCGGGGTCC GCCGCCGCTG 
    CCGCTGTCGC TGCCGCTACT GCTGCTCCTC CTGCGCGCGC AGCTCGCCGT CGGGAGCCTG
    GCCGGTGGGA GCCCCGGCGC GGCCGAGGCC CCGGGGTCGG CCCAGGTTGC TGGACTATGC
    CGGCACCTAA CCCTTCACCG GGACCTGCGC ACCGGCCGCT GGGAACCAGA CCCACAGCGC
    TCGCGACGCT GTCTCCGGGA CCCGCAGCGC GTGCTGGAGT ACTGCAGACA GATGTACCCG
    GAGCTGCAGA TTGCACGTGT GGAACAGGCG ACGCAAGCCA TCCCCATGGA GCGCTGGTGC
    GGGGGTGCCC GGGGTGGCCG CTGTGCCCAT CCCCACCACC AGGTTGTGCC TTTCCGCTGC
    CTGCCAGGTG AATTCGTGAG CGAGGCCCTG CTGGTGCCTG AAGGTTGCCG GTTCTTGCAC
    CAGGAGCGCA TGGACCAGTG TGAGAGTTCC ACCCGGAGGC ATCAAGAGGC GCAGGAGGCC
    TGCAGTTCCC AGGGCCTCAT CCTGCATGGT TCCGGCATGC TTTTGCCCTG TGGCACAGAT
    CGGTTCCGAG GTGTGGAGTA TGTGTGCTGC CCCCCGCCAG TGACCCCCGA TCCGTCTGGG
    ACAGCAGCTG GTGACCCCTC CACCCGCTCC TGGCCCCTAG GGGGCAGAGT AGAGGGGGCT
    GAGGAGGAGG AAGAAGAGGA ATCCTTCCTA CAGCCGGTGG ATGATTACTT TGTGGAACCC
    CCACGGGCTG AAGAGGAAGA TGAGGAGGAA AGAATTCTAC CCTCAAGCTC CCATAGCCCT
    GCAGGGGTCA GCAAAGTGAC CACCACTCCA AGGCCCACAG ATGGTGTGGA TGTGTACTTC
    GGCATGCCTG GAGAAATCAG CGAGCACGAG GGGTTCCTGC GGGCCAAGAT GGACCTGGAG
    GAACGAAGGA TGCGCCAGAT TAATGAGGTG ATGCGTGAAT GGGCCATGGC AGACAACCAG
    TCCAAGAACC TGCCTAAAGC AGACAGACAG GCCCTGAACG AGCACTTCCA GTCCATTCTG
    CAGACCTTGG AGGAGCAGGT ATCTGGTGAG CGACAGCGCC TGGTGGAGAC CCATGCCACC
    CGAGTCATTG CTCTCATCAA CGACCAGCGC CGGGCTGCTC TGGAAGGTTT CCTGGCGGCG
    CTGCAGGGAG ATCCACCTCA GGCAGAGCGC GTCCTGCTGG CCCTGCGGCG TTACCTGCGA
    GCAGAGCAGA AGGAACAAAG GCACACACTG AGGCACTACC AGCACGTGGC TGCTGTGGAT
    CCTGAGAAGG CCCAGCAGAT GCGCTTCCAG GTGCAGACCC ATCTTCAAGT AATTGAAGAG
    AGGATGAACC AGAGCTTGGG GCTGCTTGAC CAGAACCCCC GCCTGGCTCA GGAGCTGCGG
    CCCCAGATCC AGGAGCTCCT CCACTCTGAG CACCTGGGTC CCAATGAATT GGAAGTCCCC
    GCCCCAGGGG GCAGCAGTGA GGACAAGAGT GGGCTGCAGC CTCTGGATTC CAAAGATGCA
    GACACCCCCG TGGCTCTTCC AAAAGGGTCC ACAGAACAAG ATGCTGCATC CTCTGGGAAA
    GAGAAGATGT CCCCCCTGGA GCAGTACGAG CGAAAGGTGA ATGTGTCTGT TCCAAGGGGT
    TTTCCTTTCC ACTCATCGGA GATTCAGAGA GATGAGCTGG CACCAGCCGG GACAGGTGTG
    TCCCGAGAGG CCGTGTCTGG TCTGCTGATC ATGGGAGCAG GTGGGGGCTC CCTGATTGTC
    CTCTCCCTGC TGCTCTTGCG CAGGAAGAAG CCCTATGGGG CTATCAGCCA TGGAGTGGTG
    GAGGTGGACC CTATGCTGAC CCTGGAGGAG CAGCAGCTGC GTGAACTGCA GCGTCACGGC
    TATGAGAACC CCACCTACCG CTTCCTGGAG GAACGACCCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe02317
    Clone ID Related Accession (Same CDS sequence) XM_005616688.2 , XM_005616688.3
    Accession Version XM_005616688.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1962bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product amyloid-like protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006583.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005616688.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGGGCCCA GCAGCCCCGC CGCTCGCGGT CTGGGCCGCC GCCGGGGTCC GCCGCCGCTG 
    CCGCTGTCGC TGCCGCTACT GCTGCTCCTC CTGCGCGCGC AGCTCGCCGT CGGGAGCCTG
    GCCGGTGGGA GCCCCGGCGC GGCCGAGGCC CCGGGGTCGG CCCAGGTTGC TGGACTATGC
    CGGCACCTAA CCCTTCACCG GGACCTGCGC ACCGGCCGCT GGGAACCAGA CCCACAGCGC
    TCGCGACGCT GTCTCCGGGA CCCGCAGCGC GTGCTGGAGT ACTGCAGACA GATGTACCCG
    GAGCTGCAGA TTGCACGTGT GGAACAGGCG ACGCAAGCCA TCCCCATGGA GCGCTGGTGC
    GGGGGTGCCC GGGGTGGCCG CTGTGCCCAT CCCCACCACC AGGTTGTGCC TTTCCGCTGC
    CTGCCAGGTG AATTCGTGAG CGAGGCCCTG CTGGTGCCTG AAGGTTGCCG GTTCTTGCAC
    CAGGAGCGCA TGGACCAGTG TGAGAGTTCC ACCCGGAGGC ATCAAGAGGC GCAGGAGGCC
    TGCAGTTCCC AGGGCCTCAT CCTGCATGGT TCCGGCATGC TTTTGCCCTG TGGCACAGAT
    CGGTTCCGAG GTGTGGAGTA TGTGTGCTGC CCCCCGCCAG TGACCCCCGA TCCGTCTGGG
    ACAGCAGCTG GTGACCCCTC CACCCGCTCC TGGCCCCTAG GGGGCAGAGT AGAGGGGGCT
    GAGGAGGAGG AAGAAGAGGA ATCCTTCCTA CAGCCGGTGG ATGATTACTT TGTGGAACCC
    CCACGGGCTG AAGAGGAAGA TGAGGAGGAA AGAATTCTAC CCTCAAGCTC CCATAGCCCT
    GCAGGGGTCA GCAAAGTGAC CACCACTCCA AGGCCCACAG ATGGTGTGGA TGTGTACTTC
    GGCATGCCTG GAGAAATCAG CGAGCACGAG GGGTTCCTGC GGGCCAAGAT GGACCTGGAG
    GAACGAAGGA TGCGCCAGAT TAATGAGGTG ATGCGTGAAT GGGCCATGGC AGACAACCAG
    TCCAAGAACC TGCCTAAAGC AGACAGACAG GCCCTGAACG AGCACTTCCA GTCCATTCTG
    CAGACCTTGG AGGAGCAGGT ATCTGGTGAG CGACAGCGCC TGGTGGAGAC CCATGCCACC
    CGAGTCATTG CTCTCATCAA CGACCAGCGC CGGGCTGCTC TGGAAGGTTT CCTGGCGGCG
    CTGCAGGGAG ATCCACCTCA GGCAGAGCGC GTCCTGCTGG CCCTGCGGCG TTACCTGCGA
    GCAGAGCAGA AGGAACAAAG GCACACACTG AGGCACTACC AGCACGTGGC TGCTGTGGAT
    CCTGAGAAGG CCCAGCAGAT GCGCTTCCAG GTGCAGACCC ATCTTCAAGT AATTGAAGAG
    AGGATGAACC AGAGCTTGGG GCTGCTTGAC CAGAACCCCC GCCTGGCTCA GGAGCTGCGG
    CCCCAGATCC AGGAGCTCCT CCACTCTGAG CACCTGGGTC CCAATGAATT GGAAGTCCCC
    GCCCCAGGGG GCAGCAGTGA GGACAAGAGT GGGCTGCAGC CTCTGGATTC CAAAGATGCA
    GACACCCCCG TGGCTCTTCC AAAAGGGTCC ACAGAACAAG ATGCTGCATC CTCTGGGAAA
    GAGAAGATGT CCCCCCTGGA GCAGTACGAG CGAAAGGTGA ATGTGTCTGT TCCAAGGGGT
    TTTCCTTTCC ACTCATCGGA GATTCAGAGA GATGAGCTGG CACCAGCCGG GACAGGTGTG
    TCCCGAGAGG CCGTGTCTGG TCTGCTGATC ATGGGAGCAG GTGGGGGCTC CCTGATTGTC
    CTCTCCCTGC TGCTCTTGCG CAGGAAGAAG CCCTATGGGG CTATCAGCCA TGGAGTGGTG
    GAGGTGGACC CTATGCTGAC CCTGGAGGAG CAGCAGCTGC GTGAACTGCA GCGTCACGGC
    TATGAGAACC CCACCTACCG CTTCCTGGAG GAACGACCCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005616745.1
    CDS95..2056
    Translation

    Target ORF information:

    RefSeq Version XM_005616688.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris amyloid beta precursor like protein 1 (APLP1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005616688.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGGGCCCA GCAGCCCCGC CGCTCGCGGT CTGGGCCGCC GCCGGGGTCC GCCGCCGCTG 
    CCGCTGTCGC TGCCGCTACT GCTGCTCCTC CTGCGCGCGC AGCTCGCCGT CGGGAGCCTG
    GCCGGTGGGA GCCCCGGCGC GGCCGAGGCC CCGGGGTCGG CCCAGGTTGC TGGACTATGC
    CGGCACCTAA CCCTTCACCG GGACCTGCGC ACCGGCCGCT GGGAACCAGA CCCACAGCGC
    TCGCGACGCT GTCTCCGGGA CCCGCAGCGC GTGCTGGAGT ACTGCAGACA GATGTACCCG
    GAGCTGCAGA TTGCACGTGT GGAACAGGCG ACGCAAGCCA TCCCCATGGA GCGCTGGTGC
    GGGGGTGCCC GGGGTGGCCG CTGTGCCCAT CCCCACCACC AGGTTGTGCC TTTCCGCTGC
    CTGCCAGGTG AATTCGTGAG CGAGGCCCTG CTGGTGCCTG AAGGTTGCCG GTTCTTGCAC
    CAGGAGCGCA TGGACCAGTG TGAGAGTTCC ACCCGGAGGC ATCAAGAGGC GCAGGAGGCC
    TGCAGTTCCC AGGGCCTCAT CCTGCATGGT TCCGGCATGC TTTTGCCCTG TGGCACAGAT
    CGGTTCCGAG GTGTGGAGTA TGTGTGCTGC CCCCCGCCAG TGACCCCCGA TCCGTCTGGG
    ACAGCAGCTG GTGACCCCTC CACCCGCTCC TGGCCCCTAG GGGGCAGAGT AGAGGGGGCT
    GAGGAGGAGG AAGAAGAGGA ATCCTTCCTA CAGCCGGTGG ATGATTACTT TGTGGAACCC
    CCACGGGCTG AAGAGGAAGA TGAGGAGGAA AGAATTCTAC CCTCAAGCTC CCATAGCCCT
    GCAGGGGTCA GCAAAGTGAC CACCACTCCA AGGCCCACAG ATGGTGTGGA TGTGTACTTC
    GGCATGCCTG GAGAAATCAG CGAGCACGAG GGGTTCCTGC GGGCCAAGAT GGACCTGGAG
    GAACGAAGGA TGCGCCAGAT TAATGAGGTG ATGCGTGAAT GGGCCATGGC AGACAACCAG
    TCCAAGAACC TGCCTAAAGC AGACAGACAG GCCCTGAACG AGCACTTCCA GTCCATTCTG
    CAGACCTTGG AGGAGCAGGT ATCTGGTGAG CGACAGCGCC TGGTGGAGAC CCATGCCACC
    CGAGTCATTG CTCTCATCAA CGACCAGCGC CGGGCTGCTC TGGAAGGTTT CCTGGCGGCG
    CTGCAGGGAG ATCCACCTCA GGCAGAGCGC GTCCTGCTGG CCCTGCGGCG TTACCTGCGA
    GCAGAGCAGA AGGAACAAAG GCACACACTG AGGCACTACC AGCACGTGGC TGCTGTGGAT
    CCTGAGAAGG CCCAGCAGAT GCGCTTCCAG GTGCAGACCC ATCTTCAAGT AATTGAAGAG
    AGGATGAACC AGAGCTTGGG GCTGCTTGAC CAGAACCCCC GCCTGGCTCA GGAGCTGCGG
    CCCCAGATCC AGGAGCTCCT CCACTCTGAG CACCTGGGTC CCAATGAATT GGAAGTCCCC
    GCCCCAGGGG GCAGCAGTGA GGACAAGAGT GGGCTGCAGC CTCTGGATTC CAAAGATGCA
    GACACCCCCG TGGCTCTTCC AAAAGGGTCC ACAGAACAAG ATGCTGCATC CTCTGGGAAA
    GAGAAGATGT CCCCCCTGGA GCAGTACGAG CGAAAGGTGA ATGTGTCTGT TCCAAGGGGT
    TTTCCTTTCC ACTCATCGGA GATTCAGAGA GATGAGCTGG CACCAGCCGG GACAGGTGTG
    TCCCGAGAGG CCGTGTCTGG TCTGCTGATC ATGGGAGCAG GTGGGGGCTC CCTGATTGTC
    CTCTCCCTGC TGCTCTTGCG CAGGAAGAAG CCCTATGGGG CTATCAGCCA TGGAGTGGTG
    GAGGTGGACC CTATGCTGAC CCTGGAGGAG CAGCAGCTGC GTGAACTGCA GCGTCACGGC
    TATGAGAACC CCACCTACCG CTTCCTGGAG GAACGACCCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe02318
    Clone ID Related Accession (Same CDS sequence) XM_533688.7 , XM_533688.6
    Accession Version XM_533688.7 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1959bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product amyloid-like protein 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006583.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_533688.6. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGGGCCCA GCAGCCCCGC CGCTCGCGGT CTGGGCCGCC GCCGGGGTCC GCCGCCGCTG 
    CCGCTGTCGC TGCCGCTACT GCTGCTCCTC CTGCGCGCGC AGCTCGCCGT CGGGAGCCTG
    GCCGGTGGGA GCCCCGGCGC GGCCGAGGCC CCGGGGTCGG CCCAGGTTGC TGGACTATGC
    CGGCACCTAA CCCTTCACCG GGACCTGCGC ACCGGCCGCT GGGAACCAGA CCCACAGCGC
    TCGCGACGCT GTCTCCGGGA CCCGCAGCGC GTGCTGGAGT ACTGCAGACA GATGTACCCG
    GAGCTGCAGA TTGCACGTGT GGAACAGGCG ACGCAAGCCA TCCCCATGGA GCGCTGGTGC
    GGGGGTGCCC GGGGTGGCCG CTGTGCCCAT CCCCACCACC AGGTTGTGCC TTTCCGCTGC
    CTGCCAGGTG AATTCGTGAG CGAGGCCCTG CTGGTGCCTG AAGGTTGCCG GTTCTTGCAC
    CAGGAGCGCA TGGACCAGTG TGAGAGTTCC ACCCGGAGGC ATCAAGAGGC GCAGGAGGCC
    TGCAGTTCCC AGGGCCTCAT CCTGCATGGT TCCGGCATGC TTTTGCCCTG TGGCACAGAT
    CGGTTCCGAG GTGTGGAGTA TGTGTGCTGC CCCCCGCCAG TGACCCCCGA TCCGTCTGGG
    ACAGCAGCTG GTGACCCCTC CACCCGCTCC TGGCCCCTAG GGGGCAGAGT AGAGGGGGCT
    GAGGAGGAGG AAGAAGAGGA ATCCTTCCTA CAGCCGGTGG ATGATTACTT TGTGGAACCC
    CCACGGGCTG AAGAGGAAGA TGAGGAGGAA AGAATTCTAC CCTCAAGCTC CCATAGCCCT
    GCAGGGGTCA GCAAAGTGAC CACCACTCCA AGGCCCACAG ATGGTGTGGA TGTGTACTTC
    GGCATGCCTG GAGAAATCAG CGAGCACGAG GGGTTCCTGC GGGCCAAGAT GGACCTGGAG
    GAACGAAGGA TGCGCCAGAT TAATGAGGTG ATGCGTGAAT GGGCCATGGC AGACAACCAG
    TCCAAGAACC TGCCTAAAGC AGACAGACAG GCCCTGAACG AGCACTTCCA GTCCATTCTG
    CAGACCTTGG AGGAGCAGGT ATCTGGTGAG CGACAGCGCC TGGTGGAGAC CCATGCCACC
    CGAGTCATTG CTCTCATCAA CGACCAGCGC CGGGCTGCTC TGGAAGGTTT CCTGGCGGCG
    CTGCAGGGAG ATCCACCTCA GGCAGAGCGC GTCCTGCTGG CCCTGCGGCG TTACCTGCGA
    GCAGAGCAGA AGGAACAAAG GCACACACTG AGGCACTACC AGCACGTGGC TGCTGTGGAT
    CCTGAGAAGG CCCAGCAGAT GCGCTTCCAG GTGCAGACCC ATCTTCAAGT AATTGAAGAG
    AGGATGAACC AGAGCTTGGG GCTGCTTGAC CAGAACCCCC GCCTGGCTCA GGAGCTGCGG
    CCCCAGATCC AGGAGCTCCT CCACTCTGAG CACCTGGGTC CCAATGAATT GGAAGTCCCC
    GCCCCAGGGG GCAGCAGTGA GGACAAGAGT GGGCTGCAGC CTCTGGATTC CAAAGATGAC
    ACCCCCGTGG CTCTTCCAAA AGGGTCCACA GAACAAGATG CTGCATCCTC TGGGAAAGAG
    AAGATGTCCC CCCTGGAGCA GTACGAGCGA AAGGTGAATG TGTCTGTTCC AAGGGGTTTT
    CCTTTCCACT CATCGGAGAT TCAGAGAGAT GAGCTGGCAC CAGCCGGGAC AGGTGTGTCC
    CGAGAGGCCG TGTCTGGTCT GCTGATCATG GGAGCAGGTG GGGGCTCCCT GATTGTCCTC
    TCCCTGCTGC TCTTGCGCAG GAAGAAGCCC TATGGGGCTA TCAGCCATGG AGTGGTGGAG
    GTGGACCCTA TGCTGACCCT GGAGGAGCAG CAGCTGCGTG AACTGCAGCG TCACGGCTAT
    GAGAACCCCA CCTACCGCTT CCTGGAGGAA CGACCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_533688.4
    CDS95..2053
    Translation

    Target ORF information:

    RefSeq Version XM_533688.7
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris amyloid beta precursor like protein 1 (APLP1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_533688.7

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGGGCCCA GCAGCCCCGC CGCTCGCGGT CTGGGCCGCC GCCGGGGTCC GCCGCCGCTG 
    CCGCTGTCGC TGCCGCTACT GCTGCTCCTC CTGCGCGCGC AGCTCGCCGT CGGGAGCCTG
    GCCGGTGGGA GCCCCGGCGC GGCCGAGGCC CCGGGGTCGG CCCAGGTTGC TGGACTATGC
    CGGCACCTAA CCCTTCACCG GGACCTGCGC ACCGGCCGCT GGGAACCAGA CCCACAGCGC
    TCGCGACGCT GTCTCCGGGA CCCGCAGCGC GTGCTGGAGT ACTGCAGACA GATGTACCCG
    GAGCTGCAGA TTGCACGTGT GGAACAGGCG ACGCAAGCCA TCCCCATGGA GCGCTGGTGC
    GGGGGTGCCC GGGGTGGCCG CTGTGCCCAT CCCCACCACC AGGTTGTGCC TTTCCGCTGC
    CTGCCAGGTG AATTCGTGAG CGAGGCCCTG CTGGTGCCTG AAGGTTGCCG GTTCTTGCAC
    CAGGAGCGCA TGGACCAGTG TGAGAGTTCC ACCCGGAGGC ATCAAGAGGC GCAGGAGGCC
    TGCAGTTCCC AGGGCCTCAT CCTGCATGGT TCCGGCATGC TTTTGCCCTG TGGCACAGAT
    CGGTTCCGAG GTGTGGAGTA TGTGTGCTGC CCCCCGCCAG TGACCCCCGA TCCGTCTGGG
    ACAGCAGCTG GTGACCCCTC CACCCGCTCC TGGCCCCTAG GGGGCAGAGT AGAGGGGGCT
    GAGGAGGAGG AAGAAGAGGA ATCCTTCCTA CAGCCGGTGG ATGATTACTT TGTGGAACCC
    CCACGGGCTG AAGAGGAAGA TGAGGAGGAA AGAATTCTAC CCTCAAGCTC CCATAGCCCT
    GCAGGGGTCA GCAAAGTGAC CACCACTCCA AGGCCCACAG ATGGTGTGGA TGTGTACTTC
    GGCATGCCTG GAGAAATCAG CGAGCACGAG GGGTTCCTGC GGGCCAAGAT GGACCTGGAG
    GAACGAAGGA TGCGCCAGAT TAATGAGGTG ATGCGTGAAT GGGCCATGGC AGACAACCAG
    TCCAAGAACC TGCCTAAAGC AGACAGACAG GCCCTGAACG AGCACTTCCA GTCCATTCTG
    CAGACCTTGG AGGAGCAGGT ATCTGGTGAG CGACAGCGCC TGGTGGAGAC CCATGCCACC
    CGAGTCATTG CTCTCATCAA CGACCAGCGC CGGGCTGCTC TGGAAGGTTT CCTGGCGGCG
    CTGCAGGGAG ATCCACCTCA GGCAGAGCGC GTCCTGCTGG CCCTGCGGCG TTACCTGCGA
    GCAGAGCAGA AGGAACAAAG GCACACACTG AGGCACTACC AGCACGTGGC TGCTGTGGAT
    CCTGAGAAGG CCCAGCAGAT GCGCTTCCAG GTGCAGACCC ATCTTCAAGT AATTGAAGAG
    AGGATGAACC AGAGCTTGGG GCTGCTTGAC CAGAACCCCC GCCTGGCTCA GGAGCTGCGG
    CCCCAGATCC AGGAGCTCCT CCACTCTGAG CACCTGGGTC CCAATGAATT GGAAGTCCCC
    GCCCCAGGGG GCAGCAGTGA GGACAAGAGT GGGCTGCAGC CTCTGGATTC CAAAGATGAC
    ACCCCCGTGG CTCTTCCAAA AGGGTCCACA GAACAAGATG CTGCATCCTC TGGGAAAGAG
    AAGATGTCCC CCCTGGAGCA GTACGAGCGA AAGGTGAATG TGTCTGTTCC AAGGGGTTTT
    CCTTTCCACT CATCGGAGAT TCAGAGAGAT GAGCTGGCAC CAGCCGGGAC AGGTGTGTCC
    CGAGAGGCCG TGTCTGGTCT GCTGATCATG GGAGCAGGTG GGGGCTCCCT GATTGTCCTC
    TCCCTGCTGC TCTTGCGCAG GAAGAAGCCC TATGGGGCTA TCAGCCATGG AGTGGTGGAG
    GTGGACCCTA TGCTGACCCT GGAGGAGCAG CAGCTGCGTG AACTGCAGCG TCACGGCTAT
    GAGAACCCCA CCTACCGCTT CCTGGAGGAA CGACCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe02318
    Clone ID Related Accession (Same CDS sequence) XM_533688.7 , XM_533688.6
    Accession Version XM_533688.6 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1959bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product amyloid-like protein 1 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726047.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_533688.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGGGCCCA GCAGCCCCGC CGCTCGCGGT CTGGGCCGCC GCCGGGGTCC GCCGCCGCTG 
    CCGCTGTCGC TGCCGCTACT GCTGCTCCTC CTGCGCGCGC AGCTCGCCGT CGGGAGCCTG
    GCCGGTGGGA GCCCCGGCGC GGCCGAGGCC CCGGGGTCGG CCCAGGTTGC TGGACTATGC
    CGGCACCTAA CCCTTCACCG GGACCTGCGC ACCGGCCGCT GGGAACCAGA CCCACAGCGC
    TCGCGACGCT GTCTCCGGGA CCCGCAGCGC GTGCTGGAGT ACTGCAGACA GATGTACCCG
    GAGCTGCAGA TTGCACGTGT GGAACAGGCG ACGCAAGCCA TCCCCATGGA GCGCTGGTGC
    GGGGGTGCCC GGGGTGGCCG CTGTGCCCAT CCCCACCACC AGGTTGTGCC TTTCCGCTGC
    CTGCCAGGTG AATTCGTGAG CGAGGCCCTG CTGGTGCCTG AAGGTTGCCG GTTCTTGCAC
    CAGGAGCGCA TGGACCAGTG TGAGAGTTCC ACCCGGAGGC ATCAAGAGGC GCAGGAGGCC
    TGCAGTTCCC AGGGCCTCAT CCTGCATGGT TCCGGCATGC TTTTGCCCTG TGGCACAGAT
    CGGTTCCGAG GTGTGGAGTA TGTGTGCTGC CCCCCGCCAG TGACCCCCGA TCCGTCTGGG
    ACAGCAGCTG GTGACCCCTC CACCCGCTCC TGGCCCCTAG GGGGCAGAGT AGAGGGGGCT
    GAGGAGGAGG AAGAAGAGGA ATCCTTCCTA CAGCCGGTGG ATGATTACTT TGTGGAACCC
    CCACGGGCTG AAGAGGAAGA TGAGGAGGAA AGAATTCTAC CCTCAAGCTC CCATAGCCCT
    GCAGGGGTCA GCAAAGTGAC CACCACTCCA AGGCCCACAG ATGGTGTGGA TGTGTACTTC
    GGCATGCCTG GAGAAATCAG CGAGCACGAG GGGTTCCTGC GGGCCAAGAT GGACCTGGAG
    GAACGAAGGA TGCGCCAGAT TAATGAGGTG ATGCGTGAAT GGGCCATGGC AGACAACCAG
    TCCAAGAACC TGCCTAAAGC AGACAGACAG GCCCTGAACG AGCACTTCCA GTCCATTCTG
    CAGACCTTGG AGGAGCAGGT ATCTGGTGAG CGACAGCGCC TGGTGGAGAC CCATGCCACC
    CGAGTCATTG CTCTCATCAA CGACCAGCGC CGGGCTGCTC TGGAAGGTTT CCTGGCGGCG
    CTGCAGGGAG ATCCACCTCA GGCAGAGCGC GTCCTGCTGG CCCTGCGGCG TTACCTGCGA
    GCAGAGCAGA AGGAACAAAG GCACACACTG AGGCACTACC AGCACGTGGC TGCTGTGGAT
    CCTGAGAAGG CCCAGCAGAT GCGCTTCCAG GTGCAGACCC ATCTTCAAGT AATTGAAGAG
    AGGATGAACC AGAGCTTGGG GCTGCTTGAC CAGAACCCCC GCCTGGCTCA GGAGCTGCGG
    CCCCAGATCC AGGAGCTCCT CCACTCTGAG CACCTGGGTC CCAATGAATT GGAAGTCCCC
    GCCCCAGGGG GCAGCAGTGA GGACAAGAGT GGGCTGCAGC CTCTGGATTC CAAAGATGAC
    ACCCCCGTGG CTCTTCCAAA AGGGTCCACA GAACAAGATG CTGCATCCTC TGGGAAAGAG
    AAGATGTCCC CCCTGGAGCA GTACGAGCGA AAGGTGAATG TGTCTGTTCC AAGGGGTTTT
    CCTTTCCACT CATCGGAGAT TCAGAGAGAT GAGCTGGCAC CAGCCGGGAC AGGTGTGTCC
    CGAGAGGCCG TGTCTGGTCT GCTGATCATG GGAGCAGGTG GGGGCTCCCT GATTGTCCTC
    TCCCTGCTGC TCTTGCGCAG GAAGAAGCCC TATGGGGCTA TCAGCCATGG AGTGGTGGAG
    GTGGACCCTA TGCTGACCCT GGAGGAGCAG CAGCTGCGTG AACTGCAGCG TCACGGCTAT
    GAGAACCCCA CCTACCGCTT CCTGGAGGAA CGACCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_533688.4
    CDS96..2054
    Translation

    Target ORF information:

    RefSeq Version XM_533688.6
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris amyloid beta (A4) precursor-like protein 1 (APLP1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_533688.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGGGCCCA GCAGCCCCGC CGCTCGCGGT CTGGGCCGCC GCCGGGGTCC GCCGCCGCTG 
    CCGCTGTCGC TGCCGCTACT GCTGCTCCTC CTGCGCGCGC AGCTCGCCGT CGGGAGCCTG
    GCCGGTGGGA GCCCCGGCGC GGCCGAGGCC CCGGGGTCGG CCCAGGTTGC TGGACTATGC
    CGGCACCTAA CCCTTCACCG GGACCTGCGC ACCGGCCGCT GGGAACCAGA CCCACAGCGC
    TCGCGACGCT GTCTCCGGGA CCCGCAGCGC GTGCTGGAGT ACTGCAGACA GATGTACCCG
    GAGCTGCAGA TTGCACGTGT GGAACAGGCG ACGCAAGCCA TCCCCATGGA GCGCTGGTGC
    GGGGGTGCCC GGGGTGGCCG CTGTGCCCAT CCCCACCACC AGGTTGTGCC TTTCCGCTGC
    CTGCCAGGTG AATTCGTGAG CGAGGCCCTG CTGGTGCCTG AAGGTTGCCG GTTCTTGCAC
    CAGGAGCGCA TGGACCAGTG TGAGAGTTCC ACCCGGAGGC ATCAAGAGGC GCAGGAGGCC
    TGCAGTTCCC AGGGCCTCAT CCTGCATGGT TCCGGCATGC TTTTGCCCTG TGGCACAGAT
    CGGTTCCGAG GTGTGGAGTA TGTGTGCTGC CCCCCGCCAG TGACCCCCGA TCCGTCTGGG
    ACAGCAGCTG GTGACCCCTC CACCCGCTCC TGGCCCCTAG GGGGCAGAGT AGAGGGGGCT
    GAGGAGGAGG AAGAAGAGGA ATCCTTCCTA CAGCCGGTGG ATGATTACTT TGTGGAACCC
    CCACGGGCTG AAGAGGAAGA TGAGGAGGAA AGAATTCTAC CCTCAAGCTC CCATAGCCCT
    GCAGGGGTCA GCAAAGTGAC CACCACTCCA AGGCCCACAG ATGGTGTGGA TGTGTACTTC
    GGCATGCCTG GAGAAATCAG CGAGCACGAG GGGTTCCTGC GGGCCAAGAT GGACCTGGAG
    GAACGAAGGA TGCGCCAGAT TAATGAGGTG ATGCGTGAAT GGGCCATGGC AGACAACCAG
    TCCAAGAACC TGCCTAAAGC AGACAGACAG GCCCTGAACG AGCACTTCCA GTCCATTCTG
    CAGACCTTGG AGGAGCAGGT ATCTGGTGAG CGACAGCGCC TGGTGGAGAC CCATGCCACC
    CGAGTCATTG CTCTCATCAA CGACCAGCGC CGGGCTGCTC TGGAAGGTTT CCTGGCGGCG
    CTGCAGGGAG ATCCACCTCA GGCAGAGCGC GTCCTGCTGG CCCTGCGGCG TTACCTGCGA
    GCAGAGCAGA AGGAACAAAG GCACACACTG AGGCACTACC AGCACGTGGC TGCTGTGGAT
    CCTGAGAAGG CCCAGCAGAT GCGCTTCCAG GTGCAGACCC ATCTTCAAGT AATTGAAGAG
    AGGATGAACC AGAGCTTGGG GCTGCTTGAC CAGAACCCCC GCCTGGCTCA GGAGCTGCGG
    CCCCAGATCC AGGAGCTCCT CCACTCTGAG CACCTGGGTC CCAATGAATT GGAAGTCCCC
    GCCCCAGGGG GCAGCAGTGA GGACAAGAGT GGGCTGCAGC CTCTGGATTC CAAAGATGAC
    ACCCCCGTGG CTCTTCCAAA AGGGTCCACA GAACAAGATG CTGCATCCTC TGGGAAAGAG
    AAGATGTCCC CCCTGGAGCA GTACGAGCGA AAGGTGAATG TGTCTGTTCC AAGGGGTTTT
    CCTTTCCACT CATCGGAGAT TCAGAGAGAT GAGCTGGCAC CAGCCGGGAC AGGTGTGTCC
    CGAGAGGCCG TGTCTGGTCT GCTGATCATG GGAGCAGGTG GGGGCTCCCT GATTGTCCTC
    TCCCTGCTGC TCTTGCGCAG GAAGAAGCCC TATGGGGCTA TCAGCCATGG AGTGGTGGAG
    GTGGACCCTA TGCTGACCCT GGAGGAGCAG CAGCTGCGTG AACTGCAGCG TCACGGCTAT
    GAGAACCCCA CCTACCGCTT CCTGGAGGAA CGACCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe02319
    Clone ID Related Accession (Same CDS sequence) XM_005616689.2 , XM_005616689.3
    Accession Version XM_005616689.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1959bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product amyloid-like protein 1 isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726047.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005616689.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGGGCCCA GCAGCCCCGC CGCTCGCGGT CTGGGCCGCC GCCGGGGTCC GCCGCCGCTG 
    CCGCTGTCGC TGCCGCTACT GCTGCTCCTC CTGCGCGCGC AGCTCGCCGT CGGGAGCCTG
    GCCGGTGGGA GCCCCGGCGC GGCCGAGGCC CCGGGGTCGG CCCAGGTTGC TGGACTATGC
    CGGCACCTAA CCCTTCACCG GGACCTGCGC ACCGGCCGCT GGGAACCAGA CCCACAGCGC
    TCGCGACGCT GTCTCCGGGA CCCGCAGCGC GTGCTGGAGT ACTGCAGACA GATGTACCCG
    GAGCTGCAGA TTGCACGTGT GGAACAGGCG ACGCAAGCCA TCCCCATGGA GCGCTGGTGC
    GGGGGTGCCC GGGGTGGCCG CTGTGCCCAT CCCCACCACC AGGTTGTGCC TTTCCGCTGC
    CTGCGTGAAT TCGTGAGCGA GGCCCTGCTG GTGCCTGAAG GTTGCCGGTT CTTGCACCAG
    GAGCGCATGG ACCAGTGTGA GAGTTCCACC CGGAGGCATC AAGAGGCGCA GGAGGCCTGC
    AGTTCCCAGG GCCTCATCCT GCATGGTTCC GGCATGCTTT TGCCCTGTGG CACAGATCGG
    TTCCGAGGTG TGGAGTATGT GTGCTGCCCC CCGCCAGTGA CCCCCGATCC GTCTGGGACA
    GCAGCTGGTG ACCCCTCCAC CCGCTCCTGG CCCCTAGGGG GCAGAGTAGA GGGGGCTGAG
    GAGGAGGAAG AAGAGGAATC CTTCCTACAG CCGGTGGATG ATTACTTTGT GGAACCCCCA
    CGGGCTGAAG AGGAAGATGA GGAGGAAAGA ATTCTACCCT CAAGCTCCCA TAGCCCTGCA
    GGGGTCAGCA AAGTGACCAC CACTCCAAGG CCCACAGATG GTGTGGATGT GTACTTCGGC
    ATGCCTGGAG AAATCAGCGA GCACGAGGGG TTCCTGCGGG CCAAGATGGA CCTGGAGGAA
    CGAAGGATGC GCCAGATTAA TGAGGTGATG CGTGAATGGG CCATGGCAGA CAACCAGTCC
    AAGAACCTGC CTAAAGCAGA CAGACAGGCC CTGAACGAGC ACTTCCAGTC CATTCTGCAG
    ACCTTGGAGG AGCAGGTATC TGGTGAGCGA CAGCGCCTGG TGGAGACCCA TGCCACCCGA
    GTCATTGCTC TCATCAACGA CCAGCGCCGG GCTGCTCTGG AAGGTTTCCT GGCGGCGCTG
    CAGGGAGATC CACCTCAGGC AGAGCGCGTC CTGCTGGCCC TGCGGCGTTA CCTGCGAGCA
    GAGCAGAAGG AACAAAGGCA CACACTGAGG CACTACCAGC ACGTGGCTGC TGTGGATCCT
    GAGAAGGCCC AGCAGATGCG CTTCCAGGTG CAGACCCATC TTCAAGTAAT TGAAGAGAGG
    ATGAACCAGA GCTTGGGGCT GCTTGACCAG AACCCCCGCC TGGCTCAGGA GCTGCGGCCC
    CAGATCCAGG AGCTCCTCCA CTCTGAGCAC CTGGGTCCCA ATGAATTGGA AGTCCCCGCC
    CCAGGGGGCA GCAGTGAGGA CAAGAGTGGG CTGCAGCCTC TGGATTCCAA AGATGCAGAC
    ACCCCCGTGG CTCTTCCAAA AGGGTCCACA GAACAAGATG CTGCATCCTC TGGGAAAGAG
    AAGATGTCCC CCCTGGAGCA GTACGAGCGA AAGGTGAATG TGTCTGTTCC AAGGGGTTTT
    CCTTTCCACT CATCGGAGAT TCAGAGAGAT GAGCTGGCAC CAGCCGGGAC AGGTGTGTCC
    CGAGAGGCCG TGTCTGGTCT GCTGATCATG GGAGCAGGTG GGGGCTCCCT GATTGTCCTC
    TCCCTGCTGC TCTTGCGCAG GAAGAAGCCC TATGGGGCTA TCAGCCATGG AGTGGTGGAG
    GTGGACCCTA TGCTGACCCT GGAGGAGCAG CAGCTGCGTG AACTGCAGCG TCACGGCTAT
    GAGAACCCCA CCTACCGCTT CCTGGAGGAA CGACCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005616746.1
    CDS96..2054
    Translation

    Target ORF information:

    RefSeq Version XM_005616689.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris amyloid beta (A4) precursor-like protein 1 (APLP1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005616689.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGGGCCCA GCAGCCCCGC CGCTCGCGGT CTGGGCCGCC GCCGGGGTCC GCCGCCGCTG 
    CCGCTGTCGC TGCCGCTACT GCTGCTCCTC CTGCGCGCGC AGCTCGCCGT CGGGAGCCTG
    GCCGGTGGGA GCCCCGGCGC GGCCGAGGCC CCGGGGTCGG CCCAGGTTGC TGGACTATGC
    CGGCACCTAA CCCTTCACCG GGACCTGCGC ACCGGCCGCT GGGAACCAGA CCCACAGCGC
    TCGCGACGCT GTCTCCGGGA CCCGCAGCGC GTGCTGGAGT ACTGCAGACA GATGTACCCG
    GAGCTGCAGA TTGCACGTGT GGAACAGGCG ACGCAAGCCA TCCCCATGGA GCGCTGGTGC
    GGGGGTGCCC GGGGTGGCCG CTGTGCCCAT CCCCACCACC AGGTTGTGCC TTTCCGCTGC
    CTGCGTGAAT TCGTGAGCGA GGCCCTGCTG GTGCCTGAAG GTTGCCGGTT CTTGCACCAG
    GAGCGCATGG ACCAGTGTGA GAGTTCCACC CGGAGGCATC AAGAGGCGCA GGAGGCCTGC
    AGTTCCCAGG GCCTCATCCT GCATGGTTCC GGCATGCTTT TGCCCTGTGG CACAGATCGG
    TTCCGAGGTG TGGAGTATGT GTGCTGCCCC CCGCCAGTGA CCCCCGATCC GTCTGGGACA
    GCAGCTGGTG ACCCCTCCAC CCGCTCCTGG CCCCTAGGGG GCAGAGTAGA GGGGGCTGAG
    GAGGAGGAAG AAGAGGAATC CTTCCTACAG CCGGTGGATG ATTACTTTGT GGAACCCCCA
    CGGGCTGAAG AGGAAGATGA GGAGGAAAGA ATTCTACCCT CAAGCTCCCA TAGCCCTGCA
    GGGGTCAGCA AAGTGACCAC CACTCCAAGG CCCACAGATG GTGTGGATGT GTACTTCGGC
    ATGCCTGGAG AAATCAGCGA GCACGAGGGG TTCCTGCGGG CCAAGATGGA CCTGGAGGAA
    CGAAGGATGC GCCAGATTAA TGAGGTGATG CGTGAATGGG CCATGGCAGA CAACCAGTCC
    AAGAACCTGC CTAAAGCAGA CAGACAGGCC CTGAACGAGC ACTTCCAGTC CATTCTGCAG
    ACCTTGGAGG AGCAGGTATC TGGTGAGCGA CAGCGCCTGG TGGAGACCCA TGCCACCCGA
    GTCATTGCTC TCATCAACGA CCAGCGCCGG GCTGCTCTGG AAGGTTTCCT GGCGGCGCTG
    CAGGGAGATC CACCTCAGGC AGAGCGCGTC CTGCTGGCCC TGCGGCGTTA CCTGCGAGCA
    GAGCAGAAGG AACAAAGGCA CACACTGAGG CACTACCAGC ACGTGGCTGC TGTGGATCCT
    GAGAAGGCCC AGCAGATGCG CTTCCAGGTG CAGACCCATC TTCAAGTAAT TGAAGAGAGG
    ATGAACCAGA GCTTGGGGCT GCTTGACCAG AACCCCCGCC TGGCTCAGGA GCTGCGGCCC
    CAGATCCAGG AGCTCCTCCA CTCTGAGCAC CTGGGTCCCA ATGAATTGGA AGTCCCCGCC
    CCAGGGGGCA GCAGTGAGGA CAAGAGTGGG CTGCAGCCTC TGGATTCCAA AGATGCAGAC
    ACCCCCGTGG CTCTTCCAAA AGGGTCCACA GAACAAGATG CTGCATCCTC TGGGAAAGAG
    AAGATGTCCC CCCTGGAGCA GTACGAGCGA AAGGTGAATG TGTCTGTTCC AAGGGGTTTT
    CCTTTCCACT CATCGGAGAT TCAGAGAGAT GAGCTGGCAC CAGCCGGGAC AGGTGTGTCC
    CGAGAGGCCG TGTCTGGTCT GCTGATCATG GGAGCAGGTG GGGGCTCCCT GATTGTCCTC
    TCCCTGCTGC TCTTGCGCAG GAAGAAGCCC TATGGGGCTA TCAGCCATGG AGTGGTGGAG
    GTGGACCCTA TGCTGACCCT GGAGGAGCAG CAGCTGCGTG AACTGCAGCG TCACGGCTAT
    GAGAACCCCA CCTACCGCTT CCTGGAGGAA CGACCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe02319
    Clone ID Related Accession (Same CDS sequence) XM_005616689.2 , XM_005616689.3
    Accession Version XM_005616689.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1959bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product amyloid-like protein 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006583.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005616689.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGGGCCCA GCAGCCCCGC CGCTCGCGGT CTGGGCCGCC GCCGGGGTCC GCCGCCGCTG 
    CCGCTGTCGC TGCCGCTACT GCTGCTCCTC CTGCGCGCGC AGCTCGCCGT CGGGAGCCTG
    GCCGGTGGGA GCCCCGGCGC GGCCGAGGCC CCGGGGTCGG CCCAGGTTGC TGGACTATGC
    CGGCACCTAA CCCTTCACCG GGACCTGCGC ACCGGCCGCT GGGAACCAGA CCCACAGCGC
    TCGCGACGCT GTCTCCGGGA CCCGCAGCGC GTGCTGGAGT ACTGCAGACA GATGTACCCG
    GAGCTGCAGA TTGCACGTGT GGAACAGGCG ACGCAAGCCA TCCCCATGGA GCGCTGGTGC
    GGGGGTGCCC GGGGTGGCCG CTGTGCCCAT CCCCACCACC AGGTTGTGCC TTTCCGCTGC
    CTGCGTGAAT TCGTGAGCGA GGCCCTGCTG GTGCCTGAAG GTTGCCGGTT CTTGCACCAG
    GAGCGCATGG ACCAGTGTGA GAGTTCCACC CGGAGGCATC AAGAGGCGCA GGAGGCCTGC
    AGTTCCCAGG GCCTCATCCT GCATGGTTCC GGCATGCTTT TGCCCTGTGG CACAGATCGG
    TTCCGAGGTG TGGAGTATGT GTGCTGCCCC CCGCCAGTGA CCCCCGATCC GTCTGGGACA
    GCAGCTGGTG ACCCCTCCAC CCGCTCCTGG CCCCTAGGGG GCAGAGTAGA GGGGGCTGAG
    GAGGAGGAAG AAGAGGAATC CTTCCTACAG CCGGTGGATG ATTACTTTGT GGAACCCCCA
    CGGGCTGAAG AGGAAGATGA GGAGGAAAGA ATTCTACCCT CAAGCTCCCA TAGCCCTGCA
    GGGGTCAGCA AAGTGACCAC CACTCCAAGG CCCACAGATG GTGTGGATGT GTACTTCGGC
    ATGCCTGGAG AAATCAGCGA GCACGAGGGG TTCCTGCGGG CCAAGATGGA CCTGGAGGAA
    CGAAGGATGC GCCAGATTAA TGAGGTGATG CGTGAATGGG CCATGGCAGA CAACCAGTCC
    AAGAACCTGC CTAAAGCAGA CAGACAGGCC CTGAACGAGC ACTTCCAGTC CATTCTGCAG
    ACCTTGGAGG AGCAGGTATC TGGTGAGCGA CAGCGCCTGG TGGAGACCCA TGCCACCCGA
    GTCATTGCTC TCATCAACGA CCAGCGCCGG GCTGCTCTGG AAGGTTTCCT GGCGGCGCTG
    CAGGGAGATC CACCTCAGGC AGAGCGCGTC CTGCTGGCCC TGCGGCGTTA CCTGCGAGCA
    GAGCAGAAGG AACAAAGGCA CACACTGAGG CACTACCAGC ACGTGGCTGC TGTGGATCCT
    GAGAAGGCCC AGCAGATGCG CTTCCAGGTG CAGACCCATC TTCAAGTAAT TGAAGAGAGG
    ATGAACCAGA GCTTGGGGCT GCTTGACCAG AACCCCCGCC TGGCTCAGGA GCTGCGGCCC
    CAGATCCAGG AGCTCCTCCA CTCTGAGCAC CTGGGTCCCA ATGAATTGGA AGTCCCCGCC
    CCAGGGGGCA GCAGTGAGGA CAAGAGTGGG CTGCAGCCTC TGGATTCCAA AGATGCAGAC
    ACCCCCGTGG CTCTTCCAAA AGGGTCCACA GAACAAGATG CTGCATCCTC TGGGAAAGAG
    AAGATGTCCC CCCTGGAGCA GTACGAGCGA AAGGTGAATG TGTCTGTTCC AAGGGGTTTT
    CCTTTCCACT CATCGGAGAT TCAGAGAGAT GAGCTGGCAC CAGCCGGGAC AGGTGTGTCC
    CGAGAGGCCG TGTCTGGTCT GCTGATCATG GGAGCAGGTG GGGGCTCCCT GATTGTCCTC
    TCCCTGCTGC TCTTGCGCAG GAAGAAGCCC TATGGGGCTA TCAGCCATGG AGTGGTGGAG
    GTGGACCCTA TGCTGACCCT GGAGGAGCAG CAGCTGCGTG AACTGCAGCG TCACGGCTAT
    GAGAACCCCA CCTACCGCTT CCTGGAGGAA CGACCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005616746.1
    CDS72..2030
    Translation

    Target ORF information:

    RefSeq Version XM_005616689.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris amyloid beta precursor like protein 1 (APLP1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005616689.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGGGCCCA GCAGCCCCGC CGCTCGCGGT CTGGGCCGCC GCCGGGGTCC GCCGCCGCTG 
    CCGCTGTCGC TGCCGCTACT GCTGCTCCTC CTGCGCGCGC AGCTCGCCGT CGGGAGCCTG
    GCCGGTGGGA GCCCCGGCGC GGCCGAGGCC CCGGGGTCGG CCCAGGTTGC TGGACTATGC
    CGGCACCTAA CCCTTCACCG GGACCTGCGC ACCGGCCGCT GGGAACCAGA CCCACAGCGC
    TCGCGACGCT GTCTCCGGGA CCCGCAGCGC GTGCTGGAGT ACTGCAGACA GATGTACCCG
    GAGCTGCAGA TTGCACGTGT GGAACAGGCG ACGCAAGCCA TCCCCATGGA GCGCTGGTGC
    GGGGGTGCCC GGGGTGGCCG CTGTGCCCAT CCCCACCACC AGGTTGTGCC TTTCCGCTGC
    CTGCGTGAAT TCGTGAGCGA GGCCCTGCTG GTGCCTGAAG GTTGCCGGTT CTTGCACCAG
    GAGCGCATGG ACCAGTGTGA GAGTTCCACC CGGAGGCATC AAGAGGCGCA GGAGGCCTGC
    AGTTCCCAGG GCCTCATCCT GCATGGTTCC GGCATGCTTT TGCCCTGTGG CACAGATCGG
    TTCCGAGGTG TGGAGTATGT GTGCTGCCCC CCGCCAGTGA CCCCCGATCC GTCTGGGACA
    GCAGCTGGTG ACCCCTCCAC CCGCTCCTGG CCCCTAGGGG GCAGAGTAGA GGGGGCTGAG
    GAGGAGGAAG AAGAGGAATC CTTCCTACAG CCGGTGGATG ATTACTTTGT GGAACCCCCA
    CGGGCTGAAG AGGAAGATGA GGAGGAAAGA ATTCTACCCT CAAGCTCCCA TAGCCCTGCA
    GGGGTCAGCA AAGTGACCAC CACTCCAAGG CCCACAGATG GTGTGGATGT GTACTTCGGC
    ATGCCTGGAG AAATCAGCGA GCACGAGGGG TTCCTGCGGG CCAAGATGGA CCTGGAGGAA
    CGAAGGATGC GCCAGATTAA TGAGGTGATG CGTGAATGGG CCATGGCAGA CAACCAGTCC
    AAGAACCTGC CTAAAGCAGA CAGACAGGCC CTGAACGAGC ACTTCCAGTC CATTCTGCAG
    ACCTTGGAGG AGCAGGTATC TGGTGAGCGA CAGCGCCTGG TGGAGACCCA TGCCACCCGA
    GTCATTGCTC TCATCAACGA CCAGCGCCGG GCTGCTCTGG AAGGTTTCCT GGCGGCGCTG
    CAGGGAGATC CACCTCAGGC AGAGCGCGTC CTGCTGGCCC TGCGGCGTTA CCTGCGAGCA
    GAGCAGAAGG AACAAAGGCA CACACTGAGG CACTACCAGC ACGTGGCTGC TGTGGATCCT
    GAGAAGGCCC AGCAGATGCG CTTCCAGGTG CAGACCCATC TTCAAGTAAT TGAAGAGAGG
    ATGAACCAGA GCTTGGGGCT GCTTGACCAG AACCCCCGCC TGGCTCAGGA GCTGCGGCCC
    CAGATCCAGG AGCTCCTCCA CTCTGAGCAC CTGGGTCCCA ATGAATTGGA AGTCCCCGCC
    CCAGGGGGCA GCAGTGAGGA CAAGAGTGGG CTGCAGCCTC TGGATTCCAA AGATGCAGAC
    ACCCCCGTGG CTCTTCCAAA AGGGTCCACA GAACAAGATG CTGCATCCTC TGGGAAAGAG
    AAGATGTCCC CCCTGGAGCA GTACGAGCGA AAGGTGAATG TGTCTGTTCC AAGGGGTTTT
    CCTTTCCACT CATCGGAGAT TCAGAGAGAT GAGCTGGCAC CAGCCGGGAC AGGTGTGTCC
    CGAGAGGCCG TGTCTGGTCT GCTGATCATG GGAGCAGGTG GGGGCTCCCT GATTGTCCTC
    TCCCTGCTGC TCTTGCGCAG GAAGAAGCCC TATGGGGCTA TCAGCCATGG AGTGGTGGAG
    GTGGACCCTA TGCTGACCCT GGAGGAGCAG CAGCTGCGTG AACTGCAGCG TCACGGCTAT
    GAGAACCCCA CCTACCGCTT CCTGGAGGAA CGACCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe34642
    Clone ID Related Accession (Same CDS sequence) XM_014115534.2 , XM_014115534.1
    Accession Version XM_014115534.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1917bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product amyloid-like protein 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006583.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014115534.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGGGGCCCA GCAGCCCCGC CGCTCGCGGT CTGGGCCGCC GCCGGGGTCC GCCGCCGCTG 
    CCGCTGTCGC TGCCGCTACT GCTGCTCCTC CTGCGCGCGC AGCTCGCCGT CGGGAGCCTG
    GCCGGTGGGA GCCCCGGCGC GGCCGAGGCC CCGGGGTCGG CCCAGGTTGC TGGACTATGC
    CGGCACCTAA CCCTTCACCG GGACCTGCGC ACCGGCCGCT GGGAACCAGA CCCACAGCGC
    TCGCGACGCT GTCTCCGGGA CCCGCAGCGC GTGCTGGAGT ACTGCAGACA GATGTACCCG
    GAGCTGCAGA TTGCACGTGT GGAACAGGCG ACGCAAGCCA TCCCCATGGA GCGCTGGTGC
    GGGGGTGCCC GGGGTGGCCG CTGTGCCCAT CCCCACCACC AGGTTGTGCC TTTCCGCTGC
    CTGCCAGGTG AATTCGTGAG CGAGGCCCTG CTGGTGCCTG AAGGTTGCCG GTTCTTGCAC
    CAGGAGCGCA TGGACCAGTG TGAGAGTTCC ACCCGGAGGC ATCAAGAGGC GCAGGAGGCC
    TGCAGTTCCC AGGGCCTCAT CCTGCATGGT TCCGGCATGC TTTTGCCCTG TGGCACAGAT
    CGGTTCCGAG GTGTGGAGTA TGTGTGCTGC CCCCCGCCAG TGACCCCCGA TCCGTCTGGG
    ACAGCAGCTG GTGACCCCTC CACCCGCTCC TGGCCCCTAG GGGGCAGAGT AGAGGGGGCT
    GAGGAGGAGG AAGAAGAGGA ATCCTTCCTA CAGCCGGTGG ATGATTACTT TGTGGAACCC
    CCACGGGCTG AAGAGGAAGA TGAGGAGGAA AGAATTCTAC CCTCAAGCTC CCATAGCCCT
    GCAGGGGTCA GCAAAGTGAC CACCACTCCA AGGCCCACAG ATGGTGTGGA TGTGTACTTC
    GGCATGCCTG GAGAAATCAG CGAGCACGAG GGGTTCCTGC GGGCCAAGAT GGACCTGGAG
    GAACGAAGGA TGCGCCAGAT TAATGAGGTG ATGCGTGAAT GGGCCATGGC AGACAACCAG
    TCCAAGAACC TGCCTAAAGC AGACAGACAG GCCCTGAACG AGCACTTCCA GTCCATTCTG
    CAGACCTTGG AGGAGCAGGT ATCTGGTGAG CGACAGCGCC TGGTGGAGAC CCATGCCACC
    CGAGTCATTG CTCTCATCAA CGACCAGCGC CGGGCTGCTC TGGAAGGTTT CCTGGCGGCG
    CTGCAGGGAG ATCCACCTCA GGCAGAGCGC GTCCTGCTGG CCCTGCGGCG TTACCTGCGA
    GCAGAGCAGA AGGAACAAAG GCACACACTG AGGCACTACC AGCACGTGGC TGCTGTGGAT
    CCTGAGAAGG CCCAGCAGAT GCGCTTCCAG GTGCAGACCC ATCTTCAAGT AATTGAAGAG
    AGGATGAACC AGAGCTTGGG GCTGCTTGAC CAGAACCCCC GCCTGGCTCA GGAGCTGCGG
    CCCCAGATCC AGGAGCTCCT CCACTCTGAG CACCTGGGTC CCAATGAATT GGAAGTCCCC
    GCCCCAGGGG GCAGCAGTGA GGACAAGAGT GGGCTGCAGC CTCTGGATTC CAAAGATGCA
    GACACCCCCG TGGCTCTTCC AAAAGGGTCC ACAGAACAAG ATGCTGCATC CTCTGGGAAA
    GAGAAGATGT CCCCCCTGGA GCAGTACGAG CGAAAGGTGA ATGTGTCTGT TCCAAGGGGT
    TTTCCTTTCC ACTCATCGGA GATTCAGAGA GATGAGCTGG CACCAGCCGG GACAGGTGTG
    TCCCGAGAGG CCGTGTCTGG TCTGCTGATC ATGGGAGCAG GTGGGGGCTC CCTGATTGTC
    CTCTCCCTGC TGCTCTTGCG CAGGAAGAAG CCCTATGGGG CTATCAGCCA TGGAGTGGTG
    GAGGTCCCTA TGCCCCTCCA TCCCTCCGGC CCCTACTGCA GGTGGACCCT ATGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013971009.1
    CDS95..2011
    Translation

    Target ORF information:

    RefSeq Version XM_014115534.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris amyloid beta precursor like protein 1 (APLP1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014115534.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGGGGCCCA GCAGCCCCGC CGCTCGCGGT CTGGGCCGCC GCCGGGGTCC GCCGCCGCTG 
    CCGCTGTCGC TGCCGCTACT GCTGCTCCTC CTGCGCGCGC AGCTCGCCGT CGGGAGCCTG
    GCCGGTGGGA GCCCCGGCGC GGCCGAGGCC CCGGGGTCGG CCCAGGTTGC TGGACTATGC
    CGGCACCTAA CCCTTCACCG GGACCTGCGC ACCGGCCGCT GGGAACCAGA CCCACAGCGC
    TCGCGACGCT GTCTCCGGGA CCCGCAGCGC GTGCTGGAGT ACTGCAGACA GATGTACCCG
    GAGCTGCAGA TTGCACGTGT GGAACAGGCG ACGCAAGCCA TCCCCATGGA GCGCTGGTGC
    GGGGGTGCCC GGGGTGGCCG CTGTGCCCAT CCCCACCACC AGGTTGTGCC TTTCCGCTGC
    CTGCCAGGTG AATTCGTGAG CGAGGCCCTG CTGGTGCCTG AAGGTTGCCG GTTCTTGCAC
    CAGGAGCGCA TGGACCAGTG TGAGAGTTCC ACCCGGAGGC ATCAAGAGGC GCAGGAGGCC
    TGCAGTTCCC AGGGCCTCAT CCTGCATGGT TCCGGCATGC TTTTGCCCTG TGGCACAGAT
    CGGTTCCGAG GTGTGGAGTA TGTGTGCTGC CCCCCGCCAG TGACCCCCGA TCCGTCTGGG
    ACAGCAGCTG GTGACCCCTC CACCCGCTCC TGGCCCCTAG GGGGCAGAGT AGAGGGGGCT
    GAGGAGGAGG AAGAAGAGGA ATCCTTCCTA CAGCCGGTGG ATGATTACTT TGTGGAACCC
    CCACGGGCTG AAGAGGAAGA TGAGGAGGAA AGAATTCTAC CCTCAAGCTC CCATAGCCCT
    GCAGGGGTCA GCAAAGTGAC CACCACTCCA AGGCCCACAG ATGGTGTGGA TGTGTACTTC
    GGCATGCCTG GAGAAATCAG CGAGCACGAG GGGTTCCTGC GGGCCAAGAT GGACCTGGAG
    GAACGAAGGA TGCGCCAGAT TAATGAGGTG ATGCGTGAAT GGGCCATGGC AGACAACCAG
    TCCAAGAACC TGCCTAAAGC AGACAGACAG GCCCTGAACG AGCACTTCCA GTCCATTCTG
    CAGACCTTGG AGGAGCAGGT ATCTGGTGAG CGACAGCGCC TGGTGGAGAC CCATGCCACC
    CGAGTCATTG CTCTCATCAA CGACCAGCGC CGGGCTGCTC TGGAAGGTTT CCTGGCGGCG
    CTGCAGGGAG ATCCACCTCA GGCAGAGCGC GTCCTGCTGG CCCTGCGGCG TTACCTGCGA
    GCAGAGCAGA AGGAACAAAG GCACACACTG AGGCACTACC AGCACGTGGC TGCTGTGGAT
    CCTGAGAAGG CCCAGCAGAT GCGCTTCCAG GTGCAGACCC ATCTTCAAGT AATTGAAGAG
    AGGATGAACC AGAGCTTGGG GCTGCTTGAC CAGAACCCCC GCCTGGCTCA GGAGCTGCGG
    CCCCAGATCC AGGAGCTCCT CCACTCTGAG CACCTGGGTC CCAATGAATT GGAAGTCCCC
    GCCCCAGGGG GCAGCAGTGA GGACAAGAGT GGGCTGCAGC CTCTGGATTC CAAAGATGCA
    GACACCCCCG TGGCTCTTCC AAAAGGGTCC ACAGAACAAG ATGCTGCATC CTCTGGGAAA
    GAGAAGATGT CCCCCCTGGA GCAGTACGAG CGAAAGGTGA ATGTGTCTGT TCCAAGGGGT
    TTTCCTTTCC ACTCATCGGA GATTCAGAGA GATGAGCTGG CACCAGCCGG GACAGGTGTG
    TCCCGAGAGG CCGTGTCTGG TCTGCTGATC ATGGGAGCAG GTGGGGGCTC CCTGATTGTC
    CTCTCCCTGC TGCTCTTGCG CAGGAAGAAG CCCTATGGGG CTATCAGCCA TGGAGTGGTG
    GAGGTCCCTA TGCCCCTCCA TCCCTCCGGC CCCTACTGCA GGTGGACCCT ATGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe34642
    Clone ID Related Accession (Same CDS sequence) XM_014115534.2 , XM_014115534.1
    Accession Version XM_014115534.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1917bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product amyloid-like protein 1 isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726047.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGGGGCCCA GCAGCCCCGC CGCTCGCGGT CTGGGCCGCC GCCGGGGTCC GCCGCCGCTG 
    CCGCTGTCGC TGCCGCTACT GCTGCTCCTC CTGCGCGCGC AGCTCGCCGT CGGGAGCCTG
    GCCGGTGGGA GCCCCGGCGC GGCCGAGGCC CCGGGGTCGG CCCAGGTTGC TGGACTATGC
    CGGCACCTAA CCCTTCACCG GGACCTGCGC ACCGGCCGCT GGGAACCAGA CCCACAGCGC
    TCGCGACGCT GTCTCCGGGA CCCGCAGCGC GTGCTGGAGT ACTGCAGACA GATGTACCCG
    GAGCTGCAGA TTGCACGTGT GGAACAGGCG ACGCAAGCCA TCCCCATGGA GCGCTGGTGC
    GGGGGTGCCC GGGGTGGCCG CTGTGCCCAT CCCCACCACC AGGTTGTGCC TTTCCGCTGC
    CTGCCAGGTG AATTCGTGAG CGAGGCCCTG CTGGTGCCTG AAGGTTGCCG GTTCTTGCAC
    CAGGAGCGCA TGGACCAGTG TGAGAGTTCC ACCCGGAGGC ATCAAGAGGC GCAGGAGGCC
    TGCAGTTCCC AGGGCCTCAT CCTGCATGGT TCCGGCATGC TTTTGCCCTG TGGCACAGAT
    CGGTTCCGAG GTGTGGAGTA TGTGTGCTGC CCCCCGCCAG TGACCCCCGA TCCGTCTGGG
    ACAGCAGCTG GTGACCCCTC CACCCGCTCC TGGCCCCTAG GGGGCAGAGT AGAGGGGGCT
    GAGGAGGAGG AAGAAGAGGA ATCCTTCCTA CAGCCGGTGG ATGATTACTT TGTGGAACCC
    CCACGGGCTG AAGAGGAAGA TGAGGAGGAA AGAATTCTAC CCTCAAGCTC CCATAGCCCT
    GCAGGGGTCA GCAAAGTGAC CACCACTCCA AGGCCCACAG ATGGTGTGGA TGTGTACTTC
    GGCATGCCTG GAGAAATCAG CGAGCACGAG GGGTTCCTGC GGGCCAAGAT GGACCTGGAG
    GAACGAAGGA TGCGCCAGAT TAATGAGGTG ATGCGTGAAT GGGCCATGGC AGACAACCAG
    TCCAAGAACC TGCCTAAAGC AGACAGACAG GCCCTGAACG AGCACTTCCA GTCCATTCTG
    CAGACCTTGG AGGAGCAGGT ATCTGGTGAG CGACAGCGCC TGGTGGAGAC CCATGCCACC
    CGAGTCATTG CTCTCATCAA CGACCAGCGC CGGGCTGCTC TGGAAGGTTT CCTGGCGGCG
    CTGCAGGGAG ATCCACCTCA GGCAGAGCGC GTCCTGCTGG CCCTGCGGCG TTACCTGCGA
    GCAGAGCAGA AGGAACAAAG GCACACACTG AGGCACTACC AGCACGTGGC TGCTGTGGAT
    CCTGAGAAGG CCCAGCAGAT GCGCTTCCAG GTGCAGACCC ATCTTCAAGT AATTGAAGAG
    AGGATGAACC AGAGCTTGGG GCTGCTTGAC CAGAACCCCC GCCTGGCTCA GGAGCTGCGG
    CCCCAGATCC AGGAGCTCCT CCACTCTGAG CACCTGGGTC CCAATGAATT GGAAGTCCCC
    GCCCCAGGGG GCAGCAGTGA GGACAAGAGT GGGCTGCAGC CTCTGGATTC CAAAGATGCA
    GACACCCCCG TGGCTCTTCC AAAAGGGTCC ACAGAACAAG ATGCTGCATC CTCTGGGAAA
    GAGAAGATGT CCCCCCTGGA GCAGTACGAG CGAAAGGTGA ATGTGTCTGT TCCAAGGGGT
    TTTCCTTTCC ACTCATCGGA GATTCAGAGA GATGAGCTGG CACCAGCCGG GACAGGTGTG
    TCCCGAGAGG CCGTGTCTGG TCTGCTGATC ATGGGAGCAG GTGGGGGCTC CCTGATTGTC
    CTCTCCCTGC TGCTCTTGCG CAGGAAGAAG CCCTATGGGG CTATCAGCCA TGGAGTGGTG
    GAGGTCCCTA TGCCCCTCCA TCCCTCCGGC CCCTACTGCA GGTGGACCCT ATGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013971009.1
    CDS96..2012
    Translation

    Target ORF information:

    RefSeq Version XM_014115534.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris amyloid beta (A4) precursor-like protein 1 (APLP1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014115534.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGGGGCCCA GCAGCCCCGC CGCTCGCGGT CTGGGCCGCC GCCGGGGTCC GCCGCCGCTG 
    CCGCTGTCGC TGCCGCTACT GCTGCTCCTC CTGCGCGCGC AGCTCGCCGT CGGGAGCCTG
    GCCGGTGGGA GCCCCGGCGC GGCCGAGGCC CCGGGGTCGG CCCAGGTTGC TGGACTATGC
    CGGCACCTAA CCCTTCACCG GGACCTGCGC ACCGGCCGCT GGGAACCAGA CCCACAGCGC
    TCGCGACGCT GTCTCCGGGA CCCGCAGCGC GTGCTGGAGT ACTGCAGACA GATGTACCCG
    GAGCTGCAGA TTGCACGTGT GGAACAGGCG ACGCAAGCCA TCCCCATGGA GCGCTGGTGC
    GGGGGTGCCC GGGGTGGCCG CTGTGCCCAT CCCCACCACC AGGTTGTGCC TTTCCGCTGC
    CTGCCAGGTG AATTCGTGAG CGAGGCCCTG CTGGTGCCTG AAGGTTGCCG GTTCTTGCAC
    CAGGAGCGCA TGGACCAGTG TGAGAGTTCC ACCCGGAGGC ATCAAGAGGC GCAGGAGGCC
    TGCAGTTCCC AGGGCCTCAT CCTGCATGGT TCCGGCATGC TTTTGCCCTG TGGCACAGAT
    CGGTTCCGAG GTGTGGAGTA TGTGTGCTGC CCCCCGCCAG TGACCCCCGA TCCGTCTGGG
    ACAGCAGCTG GTGACCCCTC CACCCGCTCC TGGCCCCTAG GGGGCAGAGT AGAGGGGGCT
    GAGGAGGAGG AAGAAGAGGA ATCCTTCCTA CAGCCGGTGG ATGATTACTT TGTGGAACCC
    CCACGGGCTG AAGAGGAAGA TGAGGAGGAA AGAATTCTAC CCTCAAGCTC CCATAGCCCT
    GCAGGGGTCA GCAAAGTGAC CACCACTCCA AGGCCCACAG ATGGTGTGGA TGTGTACTTC
    GGCATGCCTG GAGAAATCAG CGAGCACGAG GGGTTCCTGC GGGCCAAGAT GGACCTGGAG
    GAACGAAGGA TGCGCCAGAT TAATGAGGTG ATGCGTGAAT GGGCCATGGC AGACAACCAG
    TCCAAGAACC TGCCTAAAGC AGACAGACAG GCCCTGAACG AGCACTTCCA GTCCATTCTG
    CAGACCTTGG AGGAGCAGGT ATCTGGTGAG CGACAGCGCC TGGTGGAGAC CCATGCCACC
    CGAGTCATTG CTCTCATCAA CGACCAGCGC CGGGCTGCTC TGGAAGGTTT CCTGGCGGCG
    CTGCAGGGAG ATCCACCTCA GGCAGAGCGC GTCCTGCTGG CCCTGCGGCG TTACCTGCGA
    GCAGAGCAGA AGGAACAAAG GCACACACTG AGGCACTACC AGCACGTGGC TGCTGTGGAT
    CCTGAGAAGG CCCAGCAGAT GCGCTTCCAG GTGCAGACCC ATCTTCAAGT AATTGAAGAG
    AGGATGAACC AGAGCTTGGG GCTGCTTGAC CAGAACCCCC GCCTGGCTCA GGAGCTGCGG
    CCCCAGATCC AGGAGCTCCT CCACTCTGAG CACCTGGGTC CCAATGAATT GGAAGTCCCC
    GCCCCAGGGG GCAGCAGTGA GGACAAGAGT GGGCTGCAGC CTCTGGATTC CAAAGATGCA
    GACACCCCCG TGGCTCTTCC AAAAGGGTCC ACAGAACAAG ATGCTGCATC CTCTGGGAAA
    GAGAAGATGT CCCCCCTGGA GCAGTACGAG CGAAAGGTGA ATGTGTCTGT TCCAAGGGGT
    TTTCCTTTCC ACTCATCGGA GATTCAGAGA GATGAGCTGG CACCAGCCGG GACAGGTGTG
    TCCCGAGAGG CCGTGTCTGG TCTGCTGATC ATGGGAGCAG GTGGGGGCTC CCTGATTGTC
    CTCTCCCTGC TGCTCTTGCG CAGGAAGAAG CCCTATGGGG CTATCAGCCA TGGAGTGGTG
    GAGGTCCCTA TGCCCCTCCA TCCCTCCGGC CCCTACTGCA GGTGGACCCT ATGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.