CUBN cDNA ORF clone, Canis lupus familiaris(dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	CUBN
Entrez Gene ID	403767
Full Name	cubilin
General protein information	Preferred Names cubilin Names cubilin
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001003148.1	NP_001003148.1	cubilin precursor

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Cubn	NP_001074553.1
Canis lupus familiaris (dog)	CUBN	NP_001003148.1
Rattus norvegicus (Norway rat)	Cubn	NP_445784.1
Danio rerio (zebrafish)	cubn	XP_002666724.2
Caenorhabditis elegans (roundworm)	ZC116.3	NP_506157.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005526	XP_315526.4
Bos taurus (cattle)	CUBN	NP_001179504.1
Gallus gallus (chicken)	CUBN	XP_001235156.3
Drosophila melanogaster (fruit fly)	CG32702	NP_727348.2
Homo sapiens (human)	CUBN	NP_001072.2
Xenopus tropicalis (tropical clawed frog)	cubn	XP_002939051.2

Gene Ontology
Bibliography

Related articles in PubMed

An exon 53 frameshift mutation in CUBN abrogates cubam function and causes Imerslund-Gr?sbeck syndrome in dogs.
Fyfe JC, Hemker SL, Venta PJ, Fitzgerald CA, Outerbridge CA, Myers SL, Giger U
Molecular genetics and metabolism109(4)390-6(2013 Aug)

Contribution of cubilin and amnionless to processing and membrane targeting of cubilin-amnionless complex.
Coudroy G, Gburek J, Kozyraki R, Madsen M, Trugnan G, Moestrup SK, Verroust PJ, Maurice M
Journal of the American Society of Nephrology : JASN16(8)2330-7(2005 Aug)

Amnionless function is required for cubilin brush-border expression and intrinsic factor-cobalamin (vitamin B12) absorption in vivo.
He Q, Madsen M, Kilkenney A, Gregory B, Christensen EI, Vorum H, H?jrup P, Sch?ffer AA, Kirkness EF, Tanner SM, de la Chapelle A, Giger U, Moestrup SK, Fyfe JC
Blood106(4)1447-53(2005 Aug)

Genetic evidence of an accessory activity required specifically for cubilin brush-border expression and intrinsic factor-cobalamin absorption.
Xu D, Kozyraki R, Newman TC, Fyfe JC
Blood94(10)3604-6(1999 Nov)

Immunocytochemical localization of the intrinsic factor-cobalamin receptor in dog-ileum: distribution of intracellular receptor during cell maturation.
Levine JS, Allen RH, Alpers DH, Seetharam B
The Journal of cell biology98(3)1111-8(1984 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

study reports results of a genome-wide positional gene approach leading to discovery of a single base deletion in CUBN causing Imerslund-Grasbeck syndrome in border collies
Title: An exon 53 frameshift mutation in CUBN abrogates cubam function and causes Imerslund-Gr?sbeck syndrome in dogs.

AMN interacts with the EGF domains of cubilin for membrane attachment and export from the endoplasmic reticulum, while extracellular cubilin does apical sorting of the complex in a carbohydrate-dependent manner
Title: Contribution of cubilin and amnionless to processing and membrane targeting of cubilin-amnionless complex.

Imerslund-Grasbeck syndromes due to an in-frame deletion of 33 nucleotides in exon 10 of AMN or a homozygous substitution in the AMN translation initiation codonabrogate AMN expression and block cubilin processing and targeting to the apical membrane.
Title: Amnionless function is required for cubilin brush-border expression and intrinsic factor-cobalamin (vitamin B12) absorption in vivo.

The following CUBN gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CUBN cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCe00367	NM_001003148.1 Latest version!	Canis lupus familiaris cubilin (CUBN), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CUBN ( NM_001003148.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> NP_001003148.1 Canis lupus familiaris cubilin (CUBN), mRNA.

Price & Availability↑

CloneID	OCe00367
Clone ID Related Accession (Same CDS sequence)	NM_001003148.1
Accession Version	NM_001003148.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 10863bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Canis lupus familiaris(dog)
Product	cubilin precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF137068.1. ##Evidence-Data-START## Transcript exon combination :: AF137068.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00013638, SAMN00991611 [ECO:0000350] ##Evidence-Data-END##

ATGTCCTCAC CTTTTCTTTG GAGTTTGATT ATTTTGTTGA CATTTGCTGA ATCAAATGGC 
GAAGCTGGAG GATTTGAACT GCAGAGACAA AAAAGAAGTA TCGACTTCCA ACAGCCTCGA 
ATGGCTACAG AGAGGGGAAA TTTAGTGTTT CTTGTTGGGT CTGCTCAAAA CATTGAATTT 
AGAACTGGAT CCCTGGGAAA AATAAAATTA AATGAGGAAG ACCTTGGTGA ATGCTTACAT 
CAGATCCAGA AAAACAAAGA GGATATTACA GATTTAAAAA GGAGTGCAGT CAATGTGCCT 
CAAAATATTT CAAGTCAAAT CCATCAGCTT AATTCTAAGC TTGTGGATCT GGAGAGAAAA 
TTTCAAAGCT TACAGCAGAC AGTTGACAAA AAGGTTTGCA GTAGCAACCC CTGCCAAAAT 
GGGGGAACCT GCCTCAATCT CCATGATTCC TTTTTCTGTA TCTGTCCCTC ACAATGGAAG 
GGCCCTCTGT GCTCAGTTGA TGTGAATGAA TGTCAGATTT ACTCAGGAAC ACCCTTGGGC 
TGCCAGAATG GAGCCACATG TGAAAATACA GCAGGGAGTT ACAGTTGTCT CTGCTCACCT 
GAGACACACG GCCCCCAGTG TGCATCAAAA TATGATGACT GTGAAGGGGG CTCCAAGGCA 
CTGTGTGTCC ATGGCATCTG TGAGGATCTA GTCCGAGTGA AGGCTGATGA GCCCAAGTAC 
AACTGCATCT GCGATGCTGG GTGGACATCC CCACTGAATA GCTCAGCCTG CGTGCTGGAC 
ATAGACGAGT GCAATCTCCA GCATGCACCA TGTTCACCTC TTGTGCAGTG CTTCAACACA 
CAAGGTTCTT TCTACTGTGG GGCCTGCCCG ACAGGCTGGC AAGGAAATGG ATATAGTTGC 
CAAGATATCG ATGAATGCAA GATAAATAAT GGCGGTTGTT CTGTGGTCCC ACCTGTCATG 
TGTGTGAATA CACTTGGGTC TTACCACTGT CAAGCGTGTC CACCAGGGTA CCAAGGCGAT 
GGCAGAGTGT GCACAGTCAT CGATATCTGC TCAGTCAATA ATGGAGGCTG CCACCCAGAA 
GCATCATGCT CCTCAGTTCT AGGTTCGTTA CCTCTCTGCA CTTGTCTCCC GGGGTATACT 
GGAAATGGTT ATGGGCCAAA TGGATGCGCG CAACTCAGTG ATACTTGCCT GAGCCACCCC 
TGTTTAAATG GCCAGTGCAT TGAAACAGTC TCTGGTTATC TTTGTAAGTG TGAATCAGGT 
TGGGCTGGCA TCAACTGTAC AGAAAATATA AATGAGTGTC TCAGCAACCC CTGCTTTAAT 
GGAGGAACTT GTGTTGATGG TGTTAATGCC TTTAGTTGTG AATGCACACG TTTCTGGACT 
GGATTCCTCT GTCAGATTCC CCAACAAGTT TGTGGAGGGT CCCTCTCGGG GATGGATGGA 
AGCTTCAGCT ATATGAGCCC TGATGTTGGT TATGTCCATG ATGTCAACTG CTTCTGGGTT 
ATCCGAACTG AAGATAGAAA GGTATTGCGT ATCACTTTCA CCTTTTTCCA GTTAGAATCA 
GTGAACAATT GTCCACATGA GTTTCTTCAG ATTCATGATG GAGATTCCTC TGCAGCACTT 
CAACTTGGAA GATTCTGTGG CTCTGTTCTC CCTCATGAAC TTCTCAGTAG TAACAATGCT 
CTCTATTTTC ATCTCTATTC TGAACATTTC AGAAGTGGGA GAGGTTTTAC AATAAGATGG 
GAAACACAGC AACCAGAGTG TGGAGGTATA CTGATGGGTA CTTATGGTTC TATTAAGTCT 
CCGGGATATC CTGGAAACTA TCCTCCAGGA AGAGATTGTG TCTGGCAAGT TGTAACCAGT 
CCTGACCTCT TGATAACGTT TACTTTTGGG ACCTTGAGTC TGGAGCACCA TGATGACTGC 
AGCAAAGATT ATCTTGAGAT TCGAGATGGT CCTTTGTATC AGGACCCCAG TCTTGGGAAA 
TTCTGCACCA CTCTCTCTGT CCCACCACTC CAGACTACAG GTCCCTTTGC CAGAGTTCAT 
TTCCATTCTG ACAACCAGAT TAATGACCAA GGCTTCCACA TCACCTACTT AACATCGCCT 
TCGGATTTGC ATTGTGGAGG AAACTACACA GACCCAGAGG GTCTTCTGTC TTCTGACTTG 
TCTGGACCTT TCACTCACAA CAGACAATGC ATCTATATTA TAAAGCAGCC CCTGGGAGAA 
CAAATACAGG TCAACTTCAC CCATGTGGAG CTGGAAGGCC AGAGCAGCTG TTCTCAGAGT 
CACATTGAGG TCCGAGATGA TAAAATCTTA CTTGGAAAAG TATGTGACAA TGAAACCCTC 
CCTCACATTA AATCCATTAG GAACCATATA TGGATCAGGC TTAAAATAGA TGCTTCTCTT 
GTAAGAGCTA GTTTCAGAGC TGTTTATCAA GTTGCTTGTG GAGGAGAATT AACTGGAGAA 
GGAGTCATTC GCTCACCTTT TTATCCTAAC GTGTATCCAG GAGAAAGAAT CTGCAGGTGG 
ACCATTCACC AGCCCCAAAG CCAAGTAGTT ATTCTCAACT TCACTGCCTT TGGAATTGAA 
AGTTCTGCCC ACTGTGATAC AGATTATATT GAGATTGGTA GCAGTTCCAT TTTGGGTTCT 
CCTGAAAATA AAAAATATTG TGGCACAGAC ATACCTTTGT TTATAACATC TGTGTACAAT 
TTTCTTTATG TCATATTCGT GAAAAGTTCT TCTACTGAAA ATCATGGTTT CATGGCTAAA 
TTCAGTGCTG CAGATTTGGC ATGTGGAGAA ATTCTTACAG AGTCAACAGG AATCATTCAA 
AGTCCTGGCC ATCCAAATAT CTACCCTCAT GGGATCAACT GTACCTGGCA TATATTAGTC 
CAACCTGGCC ACCTAATTCA TTTGATATTC AGGAAATTTC ATCTGGAGTT TCATTACAAT 
TGCACAAATG ACTATTTGGA AGTTTATGAC ACTGGTTCTA ATACATATCT TGGGAGATAT 
TGTGGGAAAT CAATCCCACC CTCTCTTACC AGTAGTACCA ACTCATTAAA GCTTATATTT 
GTGGCTGACT CTGACCTCGC TTATGAAGGC TTTTTAATAA ACTATGAAGC AACTGATGCA 
TCATCAGCAT GCATGGAAGA CTACACAGAA AACTCTGGGA CTTTTACTTC TCCAAACTTC 
CCCAATAATT ACCCCAACAA TTGGAAATGC ATTTATAGGA TCACAGTGGA AACCAGCCAA 
CAGATTGCAT TACACTTCAC AAATTTCGCT TTGGAGGAGG CCATTGGTGG ACAGTGTGTA 
GCAGATTTTG TGGAAATCAG AGATGGAGGC TATGAAACTT CACCACCCCT GGGAACATAC 
TGTGGCTCAA TTCCACCTCC AAGAATCATC TCTCACAGTA ACAAACTATG GTTACAATTT 
ACAAGTGACT TCCTGGGCTC AGGGCCTGGA TTCTCAGCTT ACTGGGATGG ATCATTAACA 
GGTTGTGGGG GTAATATCAC GACTCCCACT GGCGTGTTCA CGTCTCCCAG CTATCCAATG 
CCCTATTACC ACAGCTCTGA ATGCTATTGG TTGTTGAAAG CCAGCCACGG CAGCCCATTT 
GAACTGGAAT TTGAAGACTT TCACTTGGAG CATCACCCAA ACTGCACTTT AGATTATCTG 
GCTGTATATG ATGGCCCAAG TACCAGCTCT CACTTGCTAA GTCAGCTTTG TGGGAATGAA 
AAACCGCCTG TCATCCGTTC TACTGGAGAC AGCATGTTTT TAAAATTCAG GACAGATGAA 
GATCAACAAG GAGGTGGCTT CTTAGCCAAA TACCAACAGA CATGTAGGAA TGTAGTAATT 
GTAAACCGAA ACTATGGCAT CCTAGAGAGT ATACATTATC CAAATCCCTA TTCTGACAAT 
CAGCGTTGCA ACTGGACCAT CCAGGCAACA ACTGGCAACA CGGTGAATTA CACATTTTTG 
GCATTTGAAT TGGAAAATCA CATAAACTGC TCTACAGACT ATTTAGAGCT CTATGACGGC 
CCCAGGCGGA TGGGACGCTA CTGTGGAGCA GATATGCCGC CTACAGGGAG CACCACAGGC 
TCCAAGCTTC AAGTGCTCTT CTACACTGAT GGTGTTGGTC ACCAGGAGAA AGGATTTCAA 
ATGCAGTGGT TCATTCATGG TTGTGGTGGT GAATTGTCTG GGACCACAGG ATCCTTCAGC 
AGCCCTGGAT ACCCTAACAC ATATCCACCC AACAAGGAGT GTATCTGGTA CATTACCACA 
GCCCCTGGAA GTAGCATTCA GCTCACCATC CATGACTTTG ATGTAGAGTA TCATGCAAGA 
TGCAACTTTG ACGTCCTGGA GGTTTATGGA GGTCCTGATT TCCACTCTCC CCGAATAACC 
CAGTTGTGCT CCCAAAGATC ATCTGAGAAT CCCATGCAAG TCTCCAGCAC CGGAAATGAG 
CTAGCCATCC GCTTTAAGAC CGACAGCTCT ATAAACGGGA GAGGCTTCAA TGCCTCATGG 
CAGGCAGTCC CCGGAGGTTG TGGCGGGATT TTCCAGGCTC CCAATGGAGA GATTCATTCT 
CCAAATTACC CCAGTCCATA TAGGGGTAAC ACTGACTGCT CCTGGGTCAT TCGGGTAGAA 
AGAAATCACC GTATTCTCTT GAACTTCACT GATTTTGACC TTGAACCTCA GGACTCCTGT 
ATTACGGCAT ATGATGGTTT AAGCTCCACA ACTACCCGCC TTGCCAGTGT GTGTGGAAGA 
CAGCAGTTGA CTAACCCCAT CACCTCTTCA GGAAACAGCC TCTTTTTGCG ATTTCAATCT 
GGTCCTTCCA GACAGGGCAG GGGCTTCCGA GCTCAGTTCA ACCAAGTCTG CGGAGGCCAC 
ATCCTCACCA ACTCATTTGA TACTATATCC TCTCCATTGT TCCCTGCCAA GTATCCAAAC 
AATCAGAACT GCAGCTGGGT AATTCAAGCT CAACCTCCAT TTAACCACAT TACCCTCTCC 
TTTGACCACT TTGGACTTGA AAGCAGCACA ACGTGTACAC AGGACTTTTT AGAAATTTTG 
GATGGCGATT ATGATGATGC TCCCCTCCGA GGCCGCTACT GTGGGCACTC CATGCCGCAT 
CCCATCACTT CCTTCAGCAG CGCTCTGACG CTGAGGTTCG TCTCTGATTC TAGAGTGAAT 
TCTGATGGTT TCCATGCTAC CTATGCTGCA TCATCATCAG CTTGTGGTGG AACCTTCCAC 
ATGGCTGAAG GCATCTTCAA CAGCCCTGGC TACCCGGAAG TTTATCCCTC TAATGTGGAA 
TGTGTCTGGA ACATTGTCAG TTCCCCTGGC AACCGGCTCC AGCTGTCTTT TATAACTTTC 
CAGTTGGAGG ATTCTCAGGA CTGTAGCAGA GATTTTGTGG AGGTCCGTGA AGGAAACGCC 
ACTGGTCACT TGGTGGGGCG ATACTGTGGA AACGTTCTCC CTCTCAATTA TTCATCCATT 
GTTGGGCATA TCCTGTGGAT CAGATTTGTC TCAGATGGCT CAGGCAGTGG CACAGGCTTC 
CAAGCCACAT TTACTAAAAT ATTTGGCAAT GATAATATTG TGGGAACTCA TGGGAAAATT 
GCCTCTCCCT TATGGCCTGG GCGTTACCCC CACAACTCCA ATTACCAATG GATAGTAAAT 
GTGAATGCAA CTCAAGTTAT CCATGGTAGA ATCTTGGAGA TAGACATTGA AGGAGCACAG 
AGCTGCTATT ATGACAAATT AAGGGTTTAT GATGGCCTTG GCATTCATTC CCGCCTAATT 
GGAACTTATT GTGGTACCCA GACCACATCC TTTAGCTCCT CTAGAAATTC TTTGACATTT 
CAGTTTTCCT CTGACTCTTC AATCACAGGG AAAGGATTCC TTCTGGAGTG GTTTGCAGTG 
AATGCCTCTG GTGGACCTTT ACCTACTATT GCTACAGGTG CTTGTGGTGG CTTCCTGAGG 
ACAGGAGATG CGCCAGTCTT TCTCTTCTCC CCAGGCTGGC CAGAAAGCTA CAGTAACAGC 
GCTGACTGTA CGTGGCTTAT TCAGGCCCCA GATTCTACTG TGGAACTCAA CATCCTTTCC 
CTGGACATTG AAGCGCAGAG AACATGTGAC TATGACAAAC TTGTGATCAG AGACGGAGAT 
AGCAACCTGG CCCCACAGCT AGCAGTTCTC TGTGGCAGAG AGATCCCTGG ACCCATCCGG 
TCTACTGGAG AGTACATGTT CATCCGGTTC ACCTCAGACT TCAGCATAAC CGGAGCCGGT 
TTTAATGCAT CCTTTCACAA AAGTTGTGGT GGATATTTAC ATGCAGACAG AGGGATTATC 
ACATCTCCTC AGTATCCAGA GACCTACAGT CCCAACCTCA ACTGCTCTTG GCACGTCCTG 
GTTCAGAGTG GGCTGACCAT TGCTGTGCAT TTTGAACAGC CCTTCCAGAT TCCAAGTGGA 
GATTCTTCTT GCAGCCAGGG AGATTACTTG GTGCTGAAAA ATGGACCTGA TATCTATTCT 
CCACCCTTGG GACCCTATGG AAGAAATGGT CATTTTTGTG GCAGTCGTCC TTCATCCACT 
CTGTTCACCT CAGATAACCA AATGTTTGTT CAGTTTATTT CTGATGGTAG TAATGGAGGG 
CAAGGATTTA AGATCAAATA TGAGGCAAAG AGTTTAGCCT GTGGGGGCAA TATCTACATC 
CATGATGTGA ATTCTGCGGG ATATGTGACC TCCCCTGGCC ACCCTAACAA TTACCCCCAG 
CATGCTGATT GTAATTGGCT CATAGCTGCT CCTCCTGGAA AGCTCATAAG GGTGCAGTTT 
GAAGATCAAT TCAATATTGA AGAAACACCC AACTGTGTAT CCAATTACCT TGAGTTGCGT 
GATGGGGTTG ATTCAAATGC ACCATTACTT GCCAAGCTCT GTGGGAGATC TTTGCCCAGC 
AGTCAGTTGT CCTCAGGAGA GGTGATGTAT TTGAGGTTCC GATCTGACAA CAGCTCCACT 
CAGGTTGGGT TCAAGATCAA GTATGCAATA GCCCAGTGTG GGGGAAGAGT GACAGGGCAA 
AGTGGTATCA TTGAAAGCAG CGGATATCCA ACACTTCCAT ATAGAGACAA CTCATTCTGT 
GAGTGGCATC TCAAGGGTCC CTCAGGACAC TATCTCACCA TCCATTTTGA AGATTTTCAC 
CTTCAGAATT CCTCTGGCTG TGAAAAAGAC TTTGTGGAGA TCTGGGAAAA TCATACCTCT 
GGAAATTTAT TGGGCAGATA CTGTGGAAAC ACCATTCCTG ACAGCATAGA CACTTCTAGC 
AATGTCGCTT TGGTCAGGTT TGTCACAGAT GGTTCTGTTA CTGCCTCAGG ATTTAGACTA 
CGGTTTGAAT CCAGCATGGA AGCATGTGGT GGGGAGCTAC AGGGCCCTAC TGGAACGTTT 
ACTTCTCCCA ACTACCCAAA CCCAAATCCT CATGGCCGGG TCTGTGAGTG GAGGATCATG 
GTGCAGGAAG GAAGGAGGAT CACTCTGACT TTTAACAACC TAAGGCTGGA AGCTCATCCA 
TCCTGCTACA GTGAGCATGT GACAATATTC AATGGCATTA GAAATAACTC ACCTCAGCTA 
GAGAAACTGT GTGGTAGTGT GAACGCAAGC AGTGAGATCA AATCTTCAGG AAATACAATG 
AAAGTCGTGT TTTTCACTGA TGGATCCAGG CCATTTGGAG GCTTCAGTGC TACCTACACA 
TCCAGTGAAG ATGCAGTATG TGGTGGGTCC CTTACGCATT TCCCTGAAGG AAACTTTACT 
TCTCCTGGTT ATAATGGAGT CAGTAATTAC TCAAGAAACT TAAACTGTGA ATGGACTCTC 
AGCAATCCAA ATCAGGGAAA TTCATCTATT TACATTCATT TTGAAGATTT TTACCTGGAA 
AGTCACCAAG ACTGTCAGTT TGATGTCCTT GAGTTCCGAG TGGGTAATGC TGATGGGCCT 
CTGATGTGGA GACTGTGTGG ACCTTCAAAA CCTATAGTGC CACTGGTTAT ACCTTATCCT 
GAGGTATGGA TACACTTTGT CACCAATGAA CATGTAGAAC ATGTTGGATT CCATGCAGAG 
TATTCTTTTA CAGACTGTGG TGGAATACAG TTGGGTGAGA GTGGAGTGAT TGCAAGCCCC 
AACTATCCAG CCTCTTATGA CAGCTTGACC CATTGTTCCT GGTTGTTGGA AGCACCACAA 
GGCTTTACCA TCACTCTGAC ATTTAGTGAC TTTGATATTG AAGACCATGC AACCTGTGCT 
TGGGATTCTG TCTCTGTCAG GAATGGAGGT TCTCCTGGAT CACCCATCAT AGGACAATAC 
TGTGGGACTT CAAACCCCAG GACAATCCAG TCTGGTTCCA ACCAGCTAGT GGTGATTTTT 
AACTCGGACC ATTCTGTGCA AAATGGTGGA TTTTATGCTA CATGGAACAC ACAGACTTTA 
GGTTGTGGGG GAATACTTCA TTCAGATAAT GGTACAATCA GATCCCCTCA CTGGCCTCAG 
AATTTTCCTG AGAATAGCAG ATGTTCCTGG ACAGTCATTA CTCACGAAAG TAAGCAGTTG 
GAGATCAGCT TTGACAACAA CTTCCGAATC CCCAGTGGTG ATGGACAGTG TCAGAACAGC 
TTTGTGAAGG TGTGGGCAGG GACTGAGGAA GTCGCCGAGT CCCTGTTAGC CACGGGTTGT 
GGGAACGTGG CTCCTGGCTC CATCCTCACA CCAAGAAACG TGTTCATAGC CGTGTTCCAG 
TCTCAGGAAA CCCCAGCCCA GGGCTTCTCT GCATCCTTTG TGAGCCGATG TGGAGGTAAT 
TTCACGAACC CTTCAGGTTA CATCCTTTCT CCAAACTACC CAAGGCAATA TGACAACAAC 
ATGAATTGCA CCTACATCAT AGAGGCTGAT CCTCTGTCCG TGGTCCTCTT GACTTTTGAG 
TCTTTCCATT TGGAAGCTCG CTCAGCTATA ACTGGAAGCT GTGCCAATGA TGGTGTTCAC 
ATTATCAGGG GTTCCAACTT GTCTTCTACG CCTTTTGCCA CCGTGTGTGG CAATGAGATC 
CTGTCACCTG TCACCATCCT AGGTCCAGTG CTGCTCAACT TCTACTCAAA TGCACACACA 
ACAGACCTCG GATTCAAATT TAACTACAAG ATAACCTCCT GTGGCGGTGT ATTTAACGTC 
TCCACTGGGG TCATAAAGAG CCCTGCCTAT TCATATTCGG ACTACCCCAA CAACATATAC 
TGTCTGTACA CCATTGTTGG TAGAGACGAC AGAGTGGTTC AGCTCAAGTT TAGTGATTTT 
GATGTGGTTC CTTCCACCTT CTGCTCCCAA GACTACCTGG CAATTTATGA TGGTTCAAAC 
ATCAGTGATC CCCTTCTTGG TAAATTCTGT GGTTCCAACC TTCCACCAAA TATTAAGAGC 
AGCAATCATA GTATGCTTCT GGTATTTAAG ACCGATTCAT TTCAGACGGC AAGAGGTTGG 
AAAATAACTT TCCAGCAGAC ACTGGGGCCT CAGCAAGGAT GTGGTGGTTA TCTGACAGGT 
TCAGATAATA CCTTTGCCTC TCCTGATTCT GACTCCAATG GAAGATATGA CAAGAATTTA 
AACTGTGTAT GGTTCATAAT TGCACCAGTA AACAAACTAA TTAAACTTAC CTTCAATACA 
TTTGCTCTGG AGGCACAAAG TGCTATGCAA AGATGCATAT ATGATTATGT AAAGTTATAT 
GATGGGGATA GTGAAAATGC CAACTTGGCT GGAACATTCT GCGGTTCCAC TGTACCTGCT 
CCCTTTATCT CTTCTGGCAA CTTCCTGACG GTTCAATTTG TCAGTGATGT AACTTTAGAA 
AGGGAAGGAT TTAATGCTAC ATACACTACT GTGGACATGC CTTGTGGTGG AACATATAAT 
GCAACTTGGA CCCCACAGAG TATTTCATCA CCCAATTCAT CAAACCCAGA GGTCCCACTG 
TCCATGTGTA TGTGGTTCCT TGAAGCTCCT CCACATCAGC AGGTCAAGAT CACAGTGTGG 
GCCCTGGAGC TGCACTCACA AGACTGTGAC CAGAATTACT TAGAGTTTCG GGACTCACCT 
GAGAGTAATG GAAGTCCAGG ACCTCAGATC TGTGGCAGAA ATGCTTCGGC TACACCAACG 
TTCTATTCTT CTAGGAGTAC GGCAATTGTC ATCTTCAAGT CCGAAGTCCT CAACAGAAAC 
TCTAGAGTGG GTTTTACCTA TCAGATTGCA GGTTGTAACA GAGAATATAA TAAGGCTTTT 
GGCAATCTGA AGAGTCCCGG ATGGCCAGAT AATTATGACA ATAACTTGGA TTGCACTGTT 
ATTCTCACAG CCCCACAGAA CCATACTATT TCTCTATTTT TTCATTCATT TGGCATTGAG 
GACTCAAGTG AATGCACACA TGACTTCTTG GAGGTAAGAA ATGGAAGTGA TAGCAGTTCA 
CCATTATTTG GCACATACTG TGGAACTCTG TTGCCAGATC CTATCTTCTC TCGAAACAAC 
AAACTATACC TACGGTTTAA GACCGATAGC GCAACTTCCA ATCGTGGGTA TGAAATTGTC 
TGGACCTCAT CACCCTCTGG CTGTGGTGGA ACCCTTTATG GAGACAGTGG TTCCTTCACC 
AGCCCCGGCT ATCCCGGCAC TTACCCCAAC AACACTGACT GTGAATGGGC CATCATCGCT 
CCTGCTGGAA GACCTGTCAC CGTCACCTTT TACTTTATCA GCATCGATGA TCCCGGAGAC 
TGTGTCCAGA ACTATCTCAT ACTCTACGAT GGACCGGATG CTAATTCTCC ATCCTTTGGA 
CCATACTGTG GGGCAGACAC CAACATAGCT CCCTTTGTGG CCTCTTCACA TCGTGTCTTC 
ATAAAATTTC ACGCAGAGTA TGCAGTGTAT CCATCAGCAA TCCGATTAAC CTGGGACAGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001003148.1
CDS	74..10936
Translation	MSSPFLWSLIILLTFAESNGEAGGFELQRQKRSIDFQQPRMATERGNLVFLVGSAQNIEFRTGSLGKIKLNEEDLGECLHQIQKNKEDITDLKRSAVNVPQNISSQIHQLNSKLVDLERKFQSLQQTVDKKVCSSNPCQNGGTCLNLHDSFFCICPSQWKGPLCSVDVNECQIYSGTPLGCQNGATCENTAGSYSCLCSPETHGPQCASKYDDCEGGSKALCVHGICEDLVRVKADEPKYNCICDAGWTSPLNSSACVLDIDECNLQHAPCSPLVQCFNTQGSFYCGACPTGWQGNGYSCQDIDECKINNGGCSVVPPVMCVNTLGSYHCQACPPGYQGDGRVCTVIDICSVNNGGCHPEASCSSVLGSLPLCTCLPGYTGNGYGPNGCAQLSDTCLSHPCLNGQCIETVSGYLCKCESGWAGINCTENINECLSNPCFNGGTCVDGVNAFSCECTRFWTGFLCQIPQQVCGGSLSGMDGSFSYMSPDVGYVHDVNCFWVIRTEDRKVLRITFTFFQLESVNNCPHEFLQIHDGDSSAALQLGRFCGSVLPHELLSSNNALYFHLYSEHFRSGRGFTIRWETQQPECGGILMGTYGSIKSPGYPGNYPPGRDCVWQVVTSPDLLITFTFGTLSLEHHDDCSKDYLEIRDGPLYQDPSLGKFCTTLSVPPLQTTGPFARVHFHSDNQINDQGFHITYLTSPSDLHCGGNYTDPEGLLSSDLSGPFTHNRQCIYIIKQPLGEQIQVNFTHVELEGQSSCSQSHIEVRDDKILLGKVCDNETLPHIKSIRNHIWIRLKIDASLVRASFRAVYQVACGGELTGEGVIRSPFYPNVYPGERICRWTIHQPQSQVVILNFTAFGIESSAHCDTDYIEIGSSSILGSPENKKYCGTDIPLFITSVYNFLYVIFVKSSSTENHGFMAKFSAADLACGEILTESTGIIQSPGHPNIYPHGINCTWHILVQPGHLIHLIFRKFHLEFHYNCTNDYLEVYDTGSNTYLGRYCGKSIPPSLTSSTNSLKLIFVADSDLAYEGFLINYEATDASSACMEDYTENSGTFTSPNFPNNYPNNWKCIYRITVETSQQIALHFTNFALEEAIGGQCVADFVEIRDGGYETSPPLGTYCGSIPPPRIISHSNKLWLQFTSDFLGSGPGFSAYWDGSLTGCGGNITTPTGVFTSPSYPMPYYHSSECYWLLKASHGSPFELEFEDFHLEHHPNCTLDYLAVYDGPSTSSHLLSQLCGNEKPPVIRSTGDSMFLKFRTDEDQQGGGFLAKYQQTCRNVVIVNRNYGILESIHYPNPYSDNQRCNWTIQATTGNTVNYTFLAFELENHINCSTDYLELYDGPRRMGRYCGADMPPTGSTTGSKLQVLFYTDGVGHQEKGFQMQWFIHGCGGELSGTTGSFSSPGYPNTYPPNKECIWYITTAPGSSIQLTIHDFDVEYHARCNFDVLEVYGGPDFHSPRITQLCSQRSSENPMQVSSTGNELAIRFKTDSSINGRGFNASWQAVPGGCGGIFQAPNGEIHSPNYPSPYRGNTDCSWVIRVERNHRILLNFTDFDLEPQDSCITAYDGLSSTTTRLASVCGRQQLTNPITSSGNSLFLRFQSGPSRQGRGFRAQFNQVCGGHILTNSFDTISSPLFPAKYPNNQNCSWVIQAQPPFNHITLSFDHFGLESSTTCTQDFLEILDGDYDDAPLRGRYCGHSMPHPITSFSSALTLRFVSDSRVNSDGFHATYAASSSACGGTFHMAEGIFNSPGYPEVYPSNVECVWNIVSSPGNRLQLSFITFQLEDSQDCSRDFVEVREGNATGHLVGRYCGNVLPLNYSSIVGHILWIRFVSDGSGSGTGFQATFTKIFGNDNIVGTHGKIASPLWPGRYPHNSNYQWIVNVNATQVIHGRILEIDIEGAQSCYYDKLRVYDGLGIHSRLIGTYCGTQTTSFSSSRNSLTFQFSSDSSITGKGFLLEWFAVNASGGPLPTIATGACGGFLRTGDAPVFLFSPGWPESYSNSADCTWLIQAPDSTVELNILSLDIEAQRTCDYDKLVIRDGDSNLAPQLAVLCGREIPGPIRSTGEYMFIRFTSDFSITGAGFNASFHKSCGGYLHADRGIITSPQYPETYSPNLNCSWHVLVQSGLTIAVHFEQPFQIPSGDSSCSQGDYLVLKNGPDIYSPPLGPYGRNGHFCGSRPSSTLFTSDNQMFVQFISDGSNGGQGFKIKYEAKSLACGGNIYIHDVNSAGYVTSPGHPNNYPQHADCNWLIAAPPGKLIRVQFEDQFNIEETPNCVSNYLELRDGVDSNAPLLAKLCGRSLPSSQLSSGEVMYLRFRSDNSSTQVGFKIKYAIAQCGGRVTGQSGIIESSGYPTLPYRDNSFCEWHLKGPSGHYLTIHFEDFHLQNSSGCEKDFVEIWENHTSGNLLGRYCGNTIPDSIDTSSNVALVRFVTDGSVTASGFRLRFESSMEACGGELQGPTGTFTSPNYPNPNPHGRVCEWRIMVQEGRRITLTFNNLRLEAHPSCYSEHVTIFNGIRNNSPQLEKLCGSVNASSEIKSSGNTMKVVFFTDGSRPFGGFSATYTSSEDAVCGGSLTHFPEGNFTSPGYNGVSNYSRNLNCEWTLSNPNQGNSSIYIHFEDFYLESHQDCQFDVLEFRVGNADGPLMWRLCGPSKPIVPLVIPYPEVWIHFVTNEHVEHVGFHAEYSFTDCGGIQLGESGVIASPNYPASYDSLTHCSWLLEAPQGFTITLTFSDFDIEDHATCAWDSVSVRNGGSPGSPIIGQYCGTSNPRTIQSGSNQLVVIFNSDHSVQNGGFYATWNTQTLGCGGILHSDNGTIRSPHWPQNFPENSRCSWTVITHESKQLEISFDNNFRIPSGDGQCQNSFVKVWAGTEEVAESLLATGCGNVAPGSILTPRNVFIAVFQSQETPAQGFSASFVSRCGGNFTNPSGYILSPNYPRQYDNNMNCTYIIEADPLSVVLLTFESFHLEARSAITGSCANDGVHIIRGSNLSSTPFATVCGNEILSPVTILGPVLLNFYSNAHTTDLGFKFNYKITSCGGVFNVSTGVIKSPAYSYSDYPNNIYCLYTIVGRDDRVVQLKFSDFDVVPSTFCSQDYLAIYDGSNISDPLLGKFCGSNLPPNIKSSNHSMLLVFKTDSFQTARGWKITFQQTLGPQQGCGGYLTGSDNTFASPDSDSNGRYDKNLNCVWFIIAPVNKLIKLTFNTFALEAQSAMQRCIYDYVKLYDGDSENANLAGTFCGSTVPAPFISSGNFLTVQFVSDVTLEREGFNATYTTVDMPCGGTYNATWTPQSISSPNSSNPEVPLSMCMWFLEAPPHQQVKITVWALELHSQDCDQNYLEFRDSPESNGSPGPQICGRNASATPTFYSSRSTAIVIFKSEVLNRNSRVGFTYQIAGCNREYNKAFGNLKSPGWPDNYDNNLDCTVILTAPQNHTISLFFHSFGIEDSSECTHDFLEVRNGSDSSSPLFGTYCGTLLPDPIFSRNNKLYLRFKTDSATSNRGYEIVWTSSPSGCGGTLYGDSGSFTSPGYPGTYPNNTDCEWAIIAPAGRPVTVTFYFISIDDPGDCVQNYLILYDGPDANSPSFGPYCGADTNIAPFVASSHRVFIKFHAEYAVYPSAIRLTWDS

Target ORF information:

RefSeq Version	NM_001003148.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris cubilin (CUBN), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001003148.1

ORF Insert Sequence:

ATGTCCTCAC CTTTTCTTTG GAGTTTGATT ATTTTGTTGA CATTTGCTGA ATCAAATGGC 
GAAGCTGGAG GATTTGAACT GCAGAGACAA AAAAGAAGTA TCGACTTCCA ACAGCCTCGA 
ATGGCTACAG AGAGGGGAAA TTTAGTGTTT CTTGTTGGGT CTGCTCAAAA CATTGAATTT 
AGAACTGGAT CCCTGGGAAA AATAAAATTA AATGAGGAAG ACCTTGGTGA ATGCTTACAT 
CAGATCCAGA AAAACAAAGA GGATATTACA GATTTAAAAA GGAGTGCAGT CAATGTGCCT 
CAAAATATTT CAAGTCAAAT CCATCAGCTT AATTCTAAGC TTGTGGATCT GGAGAGAAAA 
TTTCAAAGCT TACAGCAGAC AGTTGACAAA AAGGTTTGCA GTAGCAACCC CTGCCAAAAT 
GGGGGAACCT GCCTCAATCT CCATGATTCC TTTTTCTGTA TCTGTCCCTC ACAATGGAAG 
GGCCCTCTGT GCTCAGTTGA TGTGAATGAA TGTCAGATTT ACTCAGGAAC ACCCTTGGGC 
TGCCAGAATG GAGCCACATG TGAAAATACA GCAGGGAGTT ACAGTTGTCT CTGCTCACCT 
GAGACACACG GCCCCCAGTG TGCATCAAAA TATGATGACT GTGAAGGGGG CTCCAAGGCA 
CTGTGTGTCC ATGGCATCTG TGAGGATCTA GTCCGAGTGA AGGCTGATGA GCCCAAGTAC 
AACTGCATCT GCGATGCTGG GTGGACATCC CCACTGAATA GCTCAGCCTG CGTGCTGGAC 
ATAGACGAGT GCAATCTCCA GCATGCACCA TGTTCACCTC TTGTGCAGTG CTTCAACACA 
CAAGGTTCTT TCTACTGTGG GGCCTGCCCG ACAGGCTGGC AAGGAAATGG ATATAGTTGC 
CAAGATATCG ATGAATGCAA GATAAATAAT GGCGGTTGTT CTGTGGTCCC ACCTGTCATG 
TGTGTGAATA CACTTGGGTC TTACCACTGT CAAGCGTGTC CACCAGGGTA CCAAGGCGAT 
GGCAGAGTGT GCACAGTCAT CGATATCTGC TCAGTCAATA ATGGAGGCTG CCACCCAGAA 
GCATCATGCT CCTCAGTTCT AGGTTCGTTA CCTCTCTGCA CTTGTCTCCC GGGGTATACT 
GGAAATGGTT ATGGGCCAAA TGGATGCGCG CAACTCAGTG ATACTTGCCT GAGCCACCCC 
TGTTTAAATG GCCAGTGCAT TGAAACAGTC TCTGGTTATC TTTGTAAGTG TGAATCAGGT 
TGGGCTGGCA TCAACTGTAC AGAAAATATA AATGAGTGTC TCAGCAACCC CTGCTTTAAT 
GGAGGAACTT GTGTTGATGG TGTTAATGCC TTTAGTTGTG AATGCACACG TTTCTGGACT 
GGATTCCTCT GTCAGATTCC CCAACAAGTT TGTGGAGGGT CCCTCTCGGG GATGGATGGA 
AGCTTCAGCT ATATGAGCCC TGATGTTGGT TATGTCCATG ATGTCAACTG CTTCTGGGTT 
ATCCGAACTG AAGATAGAAA GGTATTGCGT ATCACTTTCA CCTTTTTCCA GTTAGAATCA 
GTGAACAATT GTCCACATGA GTTTCTTCAG ATTCATGATG GAGATTCCTC TGCAGCACTT 
CAACTTGGAA GATTCTGTGG CTCTGTTCTC CCTCATGAAC TTCTCAGTAG TAACAATGCT 
CTCTATTTTC ATCTCTATTC TGAACATTTC AGAAGTGGGA GAGGTTTTAC AATAAGATGG 
GAAACACAGC AACCAGAGTG TGGAGGTATA CTGATGGGTA CTTATGGTTC TATTAAGTCT 
CCGGGATATC CTGGAAACTA TCCTCCAGGA AGAGATTGTG TCTGGCAAGT TGTAACCAGT 
CCTGACCTCT TGATAACGTT TACTTTTGGG ACCTTGAGTC TGGAGCACCA TGATGACTGC 
AGCAAAGATT ATCTTGAGAT TCGAGATGGT CCTTTGTATC AGGACCCCAG TCTTGGGAAA 
TTCTGCACCA CTCTCTCTGT CCCACCACTC CAGACTACAG GTCCCTTTGC CAGAGTTCAT 
TTCCATTCTG ACAACCAGAT TAATGACCAA GGCTTCCACA TCACCTACTT AACATCGCCT 
TCGGATTTGC ATTGTGGAGG AAACTACACA GACCCAGAGG GTCTTCTGTC TTCTGACTTG 
TCTGGACCTT TCACTCACAA CAGACAATGC ATCTATATTA TAAAGCAGCC CCTGGGAGAA 
CAAATACAGG TCAACTTCAC CCATGTGGAG CTGGAAGGCC AGAGCAGCTG TTCTCAGAGT 
CACATTGAGG TCCGAGATGA TAAAATCTTA CTTGGAAAAG TATGTGACAA TGAAACCCTC 
CCTCACATTA AATCCATTAG GAACCATATA TGGATCAGGC TTAAAATAGA TGCTTCTCTT 
GTAAGAGCTA GTTTCAGAGC TGTTTATCAA GTTGCTTGTG GAGGAGAATT AACTGGAGAA 
GGAGTCATTC GCTCACCTTT TTATCCTAAC GTGTATCCAG GAGAAAGAAT CTGCAGGTGG 
ACCATTCACC AGCCCCAAAG CCAAGTAGTT ATTCTCAACT TCACTGCCTT TGGAATTGAA 
AGTTCTGCCC ACTGTGATAC AGATTATATT GAGATTGGTA GCAGTTCCAT TTTGGGTTCT 
CCTGAAAATA AAAAATATTG TGGCACAGAC ATACCTTTGT TTATAACATC TGTGTACAAT 
TTTCTTTATG TCATATTCGT GAAAAGTTCT TCTACTGAAA ATCATGGTTT CATGGCTAAA 
TTCAGTGCTG CAGATTTGGC ATGTGGAGAA ATTCTTACAG AGTCAACAGG AATCATTCAA 
AGTCCTGGCC ATCCAAATAT CTACCCTCAT GGGATCAACT GTACCTGGCA TATATTAGTC 
CAACCTGGCC ACCTAATTCA TTTGATATTC AGGAAATTTC ATCTGGAGTT TCATTACAAT 
TGCACAAATG ACTATTTGGA AGTTTATGAC ACTGGTTCTA ATACATATCT TGGGAGATAT 
TGTGGGAAAT CAATCCCACC CTCTCTTACC AGTAGTACCA ACTCATTAAA GCTTATATTT 
GTGGCTGACT CTGACCTCGC TTATGAAGGC TTTTTAATAA ACTATGAAGC AACTGATGCA 
TCATCAGCAT GCATGGAAGA CTACACAGAA AACTCTGGGA CTTTTACTTC TCCAAACTTC 
CCCAATAATT ACCCCAACAA TTGGAAATGC ATTTATAGGA TCACAGTGGA AACCAGCCAA 
CAGATTGCAT TACACTTCAC AAATTTCGCT TTGGAGGAGG CCATTGGTGG ACAGTGTGTA 
GCAGATTTTG TGGAAATCAG AGATGGAGGC TATGAAACTT CACCACCCCT GGGAACATAC 
TGTGGCTCAA TTCCACCTCC AAGAATCATC TCTCACAGTA ACAAACTATG GTTACAATTT 
ACAAGTGACT TCCTGGGCTC AGGGCCTGGA TTCTCAGCTT ACTGGGATGG ATCATTAACA 
GGTTGTGGGG GTAATATCAC GACTCCCACT GGCGTGTTCA CGTCTCCCAG CTATCCAATG 
CCCTATTACC ACAGCTCTGA ATGCTATTGG TTGTTGAAAG CCAGCCACGG CAGCCCATTT 
GAACTGGAAT TTGAAGACTT TCACTTGGAG CATCACCCAA ACTGCACTTT AGATTATCTG 
GCTGTATATG ATGGCCCAAG TACCAGCTCT CACTTGCTAA GTCAGCTTTG TGGGAATGAA 
AAACCGCCTG TCATCCGTTC TACTGGAGAC AGCATGTTTT TAAAATTCAG GACAGATGAA 
GATCAACAAG GAGGTGGCTT CTTAGCCAAA TACCAACAGA CATGTAGGAA TGTAGTAATT 
GTAAACCGAA ACTATGGCAT CCTAGAGAGT ATACATTATC CAAATCCCTA TTCTGACAAT 
CAGCGTTGCA ACTGGACCAT CCAGGCAACA ACTGGCAACA CGGTGAATTA CACATTTTTG 
GCATTTGAAT TGGAAAATCA CATAAACTGC TCTACAGACT ATTTAGAGCT CTATGACGGC 
CCCAGGCGGA TGGGACGCTA CTGTGGAGCA GATATGCCGC CTACAGGGAG CACCACAGGC 
TCCAAGCTTC AAGTGCTCTT CTACACTGAT GGTGTTGGTC ACCAGGAGAA AGGATTTCAA 
ATGCAGTGGT TCATTCATGG TTGTGGTGGT GAATTGTCTG GGACCACAGG ATCCTTCAGC 
AGCCCTGGAT ACCCTAACAC ATATCCACCC AACAAGGAGT GTATCTGGTA CATTACCACA 
GCCCCTGGAA GTAGCATTCA GCTCACCATC CATGACTTTG ATGTAGAGTA TCATGCAAGA 
TGCAACTTTG ACGTCCTGGA GGTTTATGGA GGTCCTGATT TCCACTCTCC CCGAATAACC 
CAGTTGTGCT CCCAAAGATC ATCTGAGAAT CCCATGCAAG TCTCCAGCAC CGGAAATGAG 
CTAGCCATCC GCTTTAAGAC CGACAGCTCT ATAAACGGGA GAGGCTTCAA TGCCTCATGG 
CAGGCAGTCC CCGGAGGTTG TGGCGGGATT TTCCAGGCTC CCAATGGAGA GATTCATTCT 
CCAAATTACC CCAGTCCATA TAGGGGTAAC ACTGACTGCT CCTGGGTCAT TCGGGTAGAA 
AGAAATCACC GTATTCTCTT GAACTTCACT GATTTTGACC TTGAACCTCA GGACTCCTGT 
ATTACGGCAT ATGATGGTTT AAGCTCCACA ACTACCCGCC TTGCCAGTGT GTGTGGAAGA 
CAGCAGTTGA CTAACCCCAT CACCTCTTCA GGAAACAGCC TCTTTTTGCG ATTTCAATCT 
GGTCCTTCCA GACAGGGCAG GGGCTTCCGA GCTCAGTTCA ACCAAGTCTG CGGAGGCCAC 
ATCCTCACCA ACTCATTTGA TACTATATCC TCTCCATTGT TCCCTGCCAA GTATCCAAAC 
AATCAGAACT GCAGCTGGGT AATTCAAGCT CAACCTCCAT TTAACCACAT TACCCTCTCC 
TTTGACCACT TTGGACTTGA AAGCAGCACA ACGTGTACAC AGGACTTTTT AGAAATTTTG 
GATGGCGATT ATGATGATGC TCCCCTCCGA GGCCGCTACT GTGGGCACTC CATGCCGCAT 
CCCATCACTT CCTTCAGCAG CGCTCTGACG CTGAGGTTCG TCTCTGATTC TAGAGTGAAT 
TCTGATGGTT TCCATGCTAC CTATGCTGCA TCATCATCAG CTTGTGGTGG AACCTTCCAC 
ATGGCTGAAG GCATCTTCAA CAGCCCTGGC TACCCGGAAG TTTATCCCTC TAATGTGGAA 
TGTGTCTGGA ACATTGTCAG TTCCCCTGGC AACCGGCTCC AGCTGTCTTT TATAACTTTC 
CAGTTGGAGG ATTCTCAGGA CTGTAGCAGA GATTTTGTGG AGGTCCGTGA AGGAAACGCC 
ACTGGTCACT TGGTGGGGCG ATACTGTGGA AACGTTCTCC CTCTCAATTA TTCATCCATT 
GTTGGGCATA TCCTGTGGAT CAGATTTGTC TCAGATGGCT CAGGCAGTGG CACAGGCTTC 
CAAGCCACAT TTACTAAAAT ATTTGGCAAT GATAATATTG TGGGAACTCA TGGGAAAATT 
GCCTCTCCCT TATGGCCTGG GCGTTACCCC CACAACTCCA ATTACCAATG GATAGTAAAT 
GTGAATGCAA CTCAAGTTAT CCATGGTAGA ATCTTGGAGA TAGACATTGA AGGAGCACAG 
AGCTGCTATT ATGACAAATT AAGGGTTTAT GATGGCCTTG GCATTCATTC CCGCCTAATT 
GGAACTTATT GTGGTACCCA GACCACATCC TTTAGCTCCT CTAGAAATTC TTTGACATTT 
CAGTTTTCCT CTGACTCTTC AATCACAGGG AAAGGATTCC TTCTGGAGTG GTTTGCAGTG 
AATGCCTCTG GTGGACCTTT ACCTACTATT GCTACAGGTG CTTGTGGTGG CTTCCTGAGG 
ACAGGAGATG CGCCAGTCTT TCTCTTCTCC CCAGGCTGGC CAGAAAGCTA CAGTAACAGC 
GCTGACTGTA CGTGGCTTAT TCAGGCCCCA GATTCTACTG TGGAACTCAA CATCCTTTCC 
CTGGACATTG AAGCGCAGAG AACATGTGAC TATGACAAAC TTGTGATCAG AGACGGAGAT 
AGCAACCTGG CCCCACAGCT AGCAGTTCTC TGTGGCAGAG AGATCCCTGG ACCCATCCGG 
TCTACTGGAG AGTACATGTT CATCCGGTTC ACCTCAGACT TCAGCATAAC CGGAGCCGGT 
TTTAATGCAT CCTTTCACAA AAGTTGTGGT GGATATTTAC ATGCAGACAG AGGGATTATC 
ACATCTCCTC AGTATCCAGA GACCTACAGT CCCAACCTCA ACTGCTCTTG GCACGTCCTG 
GTTCAGAGTG GGCTGACCAT TGCTGTGCAT TTTGAACAGC CCTTCCAGAT TCCAAGTGGA 
GATTCTTCTT GCAGCCAGGG AGATTACTTG GTGCTGAAAA ATGGACCTGA TATCTATTCT 
CCACCCTTGG GACCCTATGG AAGAAATGGT CATTTTTGTG GCAGTCGTCC TTCATCCACT 
CTGTTCACCT CAGATAACCA AATGTTTGTT CAGTTTATTT CTGATGGTAG TAATGGAGGG 
CAAGGATTTA AGATCAAATA TGAGGCAAAG AGTTTAGCCT GTGGGGGCAA TATCTACATC 
CATGATGTGA ATTCTGCGGG ATATGTGACC TCCCCTGGCC ACCCTAACAA TTACCCCCAG 
CATGCTGATT GTAATTGGCT CATAGCTGCT CCTCCTGGAA AGCTCATAAG GGTGCAGTTT 
GAAGATCAAT TCAATATTGA AGAAACACCC AACTGTGTAT CCAATTACCT TGAGTTGCGT 
GATGGGGTTG ATTCAAATGC ACCATTACTT GCCAAGCTCT GTGGGAGATC TTTGCCCAGC 
AGTCAGTTGT CCTCAGGAGA GGTGATGTAT TTGAGGTTCC GATCTGACAA CAGCTCCACT 
CAGGTTGGGT TCAAGATCAA GTATGCAATA GCCCAGTGTG GGGGAAGAGT GACAGGGCAA 
AGTGGTATCA TTGAAAGCAG CGGATATCCA ACACTTCCAT ATAGAGACAA CTCATTCTGT 
GAGTGGCATC TCAAGGGTCC CTCAGGACAC TATCTCACCA TCCATTTTGA AGATTTTCAC 
CTTCAGAATT CCTCTGGCTG TGAAAAAGAC TTTGTGGAGA TCTGGGAAAA TCATACCTCT 
GGAAATTTAT TGGGCAGATA CTGTGGAAAC ACCATTCCTG ACAGCATAGA CACTTCTAGC 
AATGTCGCTT TGGTCAGGTT TGTCACAGAT GGTTCTGTTA CTGCCTCAGG ATTTAGACTA 
CGGTTTGAAT CCAGCATGGA AGCATGTGGT GGGGAGCTAC AGGGCCCTAC TGGAACGTTT 
ACTTCTCCCA ACTACCCAAA CCCAAATCCT CATGGCCGGG TCTGTGAGTG GAGGATCATG 
GTGCAGGAAG GAAGGAGGAT CACTCTGACT TTTAACAACC TAAGGCTGGA AGCTCATCCA 
TCCTGCTACA GTGAGCATGT GACAATATTC AATGGCATTA GAAATAACTC ACCTCAGCTA 
GAGAAACTGT GTGGTAGTGT GAACGCAAGC AGTGAGATCA AATCTTCAGG AAATACAATG 
AAAGTCGTGT TTTTCACTGA TGGATCCAGG CCATTTGGAG GCTTCAGTGC TACCTACACA 
TCCAGTGAAG ATGCAGTATG TGGTGGGTCC CTTACGCATT TCCCTGAAGG AAACTTTACT 
TCTCCTGGTT ATAATGGAGT CAGTAATTAC TCAAGAAACT TAAACTGTGA ATGGACTCTC 
AGCAATCCAA ATCAGGGAAA TTCATCTATT TACATTCATT TTGAAGATTT TTACCTGGAA 
AGTCACCAAG ACTGTCAGTT TGATGTCCTT GAGTTCCGAG TGGGTAATGC TGATGGGCCT 
CTGATGTGGA GACTGTGTGG ACCTTCAAAA CCTATAGTGC CACTGGTTAT ACCTTATCCT 
GAGGTATGGA TACACTTTGT CACCAATGAA CATGTAGAAC ATGTTGGATT CCATGCAGAG 
TATTCTTTTA CAGACTGTGG TGGAATACAG TTGGGTGAGA GTGGAGTGAT TGCAAGCCCC 
AACTATCCAG CCTCTTATGA CAGCTTGACC CATTGTTCCT GGTTGTTGGA AGCACCACAA 
GGCTTTACCA TCACTCTGAC ATTTAGTGAC TTTGATATTG AAGACCATGC AACCTGTGCT 
TGGGATTCTG TCTCTGTCAG GAATGGAGGT TCTCCTGGAT CACCCATCAT AGGACAATAC 
TGTGGGACTT CAAACCCCAG GACAATCCAG TCTGGTTCCA ACCAGCTAGT GGTGATTTTT 
AACTCGGACC ATTCTGTGCA AAATGGTGGA TTTTATGCTA CATGGAACAC ACAGACTTTA 
GGTTGTGGGG GAATACTTCA TTCAGATAAT GGTACAATCA GATCCCCTCA CTGGCCTCAG 
AATTTTCCTG AGAATAGCAG ATGTTCCTGG ACAGTCATTA CTCACGAAAG TAAGCAGTTG 
GAGATCAGCT TTGACAACAA CTTCCGAATC CCCAGTGGTG ATGGACAGTG TCAGAACAGC 
TTTGTGAAGG TGTGGGCAGG GACTGAGGAA GTCGCCGAGT CCCTGTTAGC CACGGGTTGT 
GGGAACGTGG CTCCTGGCTC CATCCTCACA CCAAGAAACG TGTTCATAGC CGTGTTCCAG 
TCTCAGGAAA CCCCAGCCCA GGGCTTCTCT GCATCCTTTG TGAGCCGATG TGGAGGTAAT 
TTCACGAACC CTTCAGGTTA CATCCTTTCT CCAAACTACC CAAGGCAATA TGACAACAAC 
ATGAATTGCA CCTACATCAT AGAGGCTGAT CCTCTGTCCG TGGTCCTCTT GACTTTTGAG 
TCTTTCCATT TGGAAGCTCG CTCAGCTATA ACTGGAAGCT GTGCCAATGA TGGTGTTCAC 
ATTATCAGGG GTTCCAACTT GTCTTCTACG CCTTTTGCCA CCGTGTGTGG CAATGAGATC 
CTGTCACCTG TCACCATCCT AGGTCCAGTG CTGCTCAACT TCTACTCAAA TGCACACACA 
ACAGACCTCG GATTCAAATT TAACTACAAG ATAACCTCCT GTGGCGGTGT ATTTAACGTC 
TCCACTGGGG TCATAAAGAG CCCTGCCTAT TCATATTCGG ACTACCCCAA CAACATATAC 
TGTCTGTACA CCATTGTTGG TAGAGACGAC AGAGTGGTTC AGCTCAAGTT TAGTGATTTT 
GATGTGGTTC CTTCCACCTT CTGCTCCCAA GACTACCTGG CAATTTATGA TGGTTCAAAC 
ATCAGTGATC CCCTTCTTGG TAAATTCTGT GGTTCCAACC TTCCACCAAA TATTAAGAGC 
AGCAATCATA GTATGCTTCT GGTATTTAAG ACCGATTCAT TTCAGACGGC AAGAGGTTGG 
AAAATAACTT TCCAGCAGAC ACTGGGGCCT CAGCAAGGAT GTGGTGGTTA TCTGACAGGT 
TCAGATAATA CCTTTGCCTC TCCTGATTCT GACTCCAATG GAAGATATGA CAAGAATTTA 
AACTGTGTAT GGTTCATAAT TGCACCAGTA AACAAACTAA TTAAACTTAC CTTCAATACA 
TTTGCTCTGG AGGCACAAAG TGCTATGCAA AGATGCATAT ATGATTATGT AAAGTTATAT 
GATGGGGATA GTGAAAATGC CAACTTGGCT GGAACATTCT GCGGTTCCAC TGTACCTGCT 
CCCTTTATCT CTTCTGGCAA CTTCCTGACG GTTCAATTTG TCAGTGATGT AACTTTAGAA 
AGGGAAGGAT TTAATGCTAC ATACACTACT GTGGACATGC CTTGTGGTGG AACATATAAT 
GCAACTTGGA CCCCACAGAG TATTTCATCA CCCAATTCAT CAAACCCAGA GGTCCCACTG 
TCCATGTGTA TGTGGTTCCT TGAAGCTCCT CCACATCAGC AGGTCAAGAT CACAGTGTGG 
GCCCTGGAGC TGCACTCACA AGACTGTGAC CAGAATTACT TAGAGTTTCG GGACTCACCT 
GAGAGTAATG GAAGTCCAGG ACCTCAGATC TGTGGCAGAA ATGCTTCGGC TACACCAACG 
TTCTATTCTT CTAGGAGTAC GGCAATTGTC ATCTTCAAGT CCGAAGTCCT CAACAGAAAC 
TCTAGAGTGG GTTTTACCTA TCAGATTGCA GGTTGTAACA GAGAATATAA TAAGGCTTTT 
GGCAATCTGA AGAGTCCCGG ATGGCCAGAT AATTATGACA ATAACTTGGA TTGCACTGTT 
ATTCTCACAG CCCCACAGAA CCATACTATT TCTCTATTTT TTCATTCATT TGGCATTGAG 
GACTCAAGTG AATGCACACA TGACTTCTTG GAGGTAAGAA ATGGAAGTGA TAGCAGTTCA 
CCATTATTTG GCACATACTG TGGAACTCTG TTGCCAGATC CTATCTTCTC TCGAAACAAC 
AAACTATACC TACGGTTTAA GACCGATAGC GCAACTTCCA ATCGTGGGTA TGAAATTGTC 
TGGACCTCAT CACCCTCTGG CTGTGGTGGA ACCCTTTATG GAGACAGTGG TTCCTTCACC 
AGCCCCGGCT ATCCCGGCAC TTACCCCAAC AACACTGACT GTGAATGGGC CATCATCGCT 
CCTGCTGGAA GACCTGTCAC CGTCACCTTT TACTTTATCA GCATCGATGA TCCCGGAGAC 
TGTGTCCAGA ACTATCTCAT ACTCTACGAT GGACCGGATG CTAATTCTCC ATCCTTTGGA 
CCATACTGTG GGGCAGACAC CAACATAGCT CCCTTTGTGG CCTCTTCACA TCGTGTCTTC 
ATAAAATTTC ACGCAGAGTA TGCAGTGTAT CCATCAGCAA TCCGATTAAC CTGGGACAGC 
TGA