Cubn cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cubn
Entrez Gene ID	326235
Full Name	Cubilin
Synonyms	CG2996,CG32702,CT7882,Dmel\CG32702,dCubn,tok-like
General protein information	Preferred Names Cubilin Names CG32702 gene product from transcript CG32702-RB CG32702-PB Cubn-PB TMPRSS6 iron sensing cubilin cubilin ortholog dCubilin
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 8E7-8E10

mRNA and Protein(s)

mRNA	Protein	Name
NM_167193.2	NP_727348.2	cubilin

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Cubn	NP_001074553.1
Canis lupus familiaris (dog)	CUBN	NP_001003148.1
Rattus norvegicus (Norway rat)	Cubn	NP_445784.1
Danio rerio (zebrafish)	cubn	XP_002666724.2
Caenorhabditis elegans (roundworm)	ZC116.3	NP_506157.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005526	XP_315526.4
Bos taurus (cattle)	CUBN	NP_001179504.1
Gallus gallus (chicken)	CUBN	XP_001235156.3
Drosophila melanogaster (fruit fly)	CG32702	NP_727348.2
Homo sapiens (human)	CUBN	NP_001072.2
Xenopus tropicalis (tropical clawed frog)	cubn	XP_002939051.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

We screened for genes required for nephrocyte function and identified two Drosophila genes encoding orthologs of mammalian cubilin and amnionless (AMN), two major receptors for protein reabsorption in the proximal tubule.
Title: Cubilin and amnionless mediate protein reabsorption in Drosophila nephrocytes.

The following Cubn gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cubn cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa02529	NM_167193.2 Latest version!	Drosophila melanogaster cubilin (Cubn), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cubn ( NM_167193.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_727348.2 Drosophila melanogaster cubilin (Cubn), partial mRNA.

Price & Availability↑

CloneID	OFa02529
Clone ID Related Accession (Same CDS sequence)	NM_167193.2
Accession Version	NM_167193.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 11253bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	cubilin
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jan 26, 2009 this sequence version replaced NM_167193.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on the 5' end.

ATGGAAGGAG CTGCGAGGAG CAGACTCCTA TTGTGTTGGA CACTCCTTGC CATCATCACA 
GATACTTGGC CCATTGCCGA GGGTTTTGTC AACTCGCCAA AAATTATAAG CAAAGATGGC 
AATTTGATTT TTGAATCCGG TGCAAATCGC AATATAAGCT TTCGATTGAG CGGAAATTCG 
CGGCTTACCA TCAACGAGGA ATTGGATGTT ATGGAGCTCT TGCTGGCCAC CAGTGGCTCC 
AAAAAGCGTT CCGGTGGCAA GGATGATGAT TTCGTAGATG CGCGCGAGCT GGCGGATCAG 
TTGGCTGATT TCAATAGGCG CGCCTTTGGA GCGAATGGCT TGAGTGCAAT GCTGCGAGTG 
CAGCAAAATA GGACCCGAGG ATCGATGGCT CTGTTGCGGC GTTTTCAGAC ACGACTCAGA 
GCGCTGGAGA ATCGGGTGGA TCGAATGAAG ACCGATTTGG AGGCAAACAG CTGTGCAAGT 
GGACCCTGCG AGAATGGAGG CACCTGCTAC AACACATACA CCGGATTCCG TTGCCAATGT 
CGATCGGCAT TCGAGGGTAC CAAGTGCGAG ATGGATGTCA ACGAGTGCGC ACTGTACGAG 
GGCACCGATC TGGGTTGCCA AAATGGTGGA CAATGCCAGA ATCATTTCGG CACATACAGT 
TGCCTTTGCC AGCCGGGTTG GCATGGGATG CACTGCACCC AGCGCAAGGC GGATTGCTCA 
CAGTCCAGTG CATGGGAGCT GTGTGGCCAT GGATCTTGTG TGCCCAGTGC AGACGATGCC 
GGCTATCGAT GCATCTGCGA ACCGGGTTGG AAGACGAATG GCTTGACACC CATTTGTGGC 
GAGGATGTGG ACGAATGCAG CGATTCGGCC GCCCACAAGC CCTGCTCCAC CAGCTGCATT 
AATCTGCCTG GCAGCTTCAC CTGTGCTCCC TGTCCAGCCG GCTTGACCGG GAATGGGGTC 
AGTTGCCGGG ACTTGGACGA ATGCCAGACG AACAACGGTG GATGCAGTCT TAGTCCCAAG 
GTAGATTGCA TAAATACCTA TGGTTCATAT CATTGCGGCG AGTGTCCCGT TGGCTGGACC 
GGCGATGGGC GGAAGTGCGA GAGAAGCCCC CAGGACATTG ACATCCCAGC TGGACAGACG 
CCAAGAACTT GTCCGGCGGG CAACAATCCC TGCTATCCGA CGGCGAGCTG CTTTTTGATC 
TCCGGCACCA CATCCTGCAG ATGTCCAATG GGCATGGTGG GAACAGGATA CGGTCCGAAT 
GGCTGTGTCA ATGGCACGAC CACAAACTGC AAGGAAAATC CCTGCCTGAA TGGTGGCATT 
TGCCTGTTCG CCGGACCCTC GAATTATACC TGCCTGTGTC CAATTGGCTT CCGTCCGCCC 
ATCTGTGAGC CACAGCCGAG TCCTTGCGAT CAGCATCCAT GCAAGAATGG AGGACGTTGC 
CGTCCCACCA CCAGTGGGGA TCTATTCGTC TGCCAGTGTT TACCTGGCTA CCGGGGACGA 
CTTTGCGAGA CGCGCTTCAG CAGCTGCAAT GGAATGCTGA GTGCCCAGAG TGGCAGGCTT 
AGGTATCCGC CGGAGGGCAC TGGATACGAG CACAATGCCC AGTGCGCTTG GGTTATTCGC 
ACCAACGAAT CGCTGGTGGT AAATGTCACG TTCAATAGCT TCGATGTGGA GGATTCCACG 
GAATGCCGCT TCGATTGGCT GCAGATCAAC GATGGCCGTT CGGCGGCTGC CCAGATAATT 
GGACGCTATT GTGGTAACCA TTTACCGCAT GGCGGGAATA TCGTATCATC CGGCAATCAA 
CTCTATCTAT GGTTCCGATC CGATAACTCG ACGGCTAAAG AGGGATTCGA TCTCACATGG 
AACTCGATGG AGCCGCAGTG TGGCGGTCGC TTGAATTTCG AGACACATGG CACACTTGCG 
TCGCCGGGAT CGCCCGGAAA TTATCCGAAG AATCGGGACT GCCGGTGGCA ACTGGTGGCG 
CCCACCACCA AACGCATCAA GCTGACCTTC TTCAGTTTGC AGCTGGAGCA GCACGCAAAT 
TGCAATTTCG ATTATGTCCT GATCAAGGAC AGCATTTCCG GACGTGAGCT GGCCAAATAT 
TGCACCACGG GAGCGCCAGC ACCGCTGCTC TTGCCCACCC ACCTGGCCGA GATCCACTTC 
CATTCGGATG CCGAGGGCAG CGATACTGGC TTCCAGCTGC ACTATTCCGT GGAGGAGCGT 
GTGCCCGGCT GCGGTGGTGT CTACACCGCC AAGGAGGGCA CCATTTCTGA ATCATCCACA 
GCCAATACCG AACCAGGTGG TGTCTCCTGC GAATACGAAA TCCATTTGGC CGTGGGCGAG 
CAGGTTGTCA TTCAGTTTGC GCGTCTGGAG CTGGATCCAC TTGACTGCCT GGAGGTGCTG 
GATATCACGG ACGAGGGTGG TAGCATTTTG CAGGAGAAGA TTTGCGGATC GGATGCGTCG 
CGTTTGAATC CTCCTACCTT CACTTCGGAA TTCAATCGGC TGAAGATTAA GTTTTATGCC 
CGTGCCGGCA GCTTCCAGTT GAACTACCGG ATGGCCTGCG ATTATAAACT GAATAATGAG 
CAGGGCACCA TTACCTCACC CGGTTATCCC AATCTAACAA GATCGGATCG TATCTGCACC 
TATACGATTA GTACGGCCAC CAATACGGTG ATTAGTCTGA AGAGGATCGA CTTTCAGTTG 
ACCAATGGCG AGAGCGACGA TGATGATAAC GATGAATGTC TGACAACCAA CTTGAGAATC 
AACGATGGAT TAAACCGAAA GATCCTGGGC CCCTATTGCG GCAAGAATCA GCCGGAGGAG 
AACTTTGTCA GCGAAACCAA CTACCTGCAG TTGCACCTGT CCACCGATGT GGACAGCATG 
GGGCGTGGCT TTAAGTTCGA GTATCGAGCC CTGGCCACCG GCAACGATAA GTGCGGTGGC 
GTTCATACCC GTTCCGGCGA TCATATCCGC TTGCCGGTGC ACGACGACTC ATATGCCGGC 
GAAGCCACCT GTTACTGGGT GATAATGGCG CCGGCAAACA AGGCCATCCG CCTCCACTGG 
AACAGCTTCA GTTTGGAAAA TGCGGTGGAC TGCATCTATG ACTATTTGGA GATCTATGAT 
AGTCTGGGTG CCCAGGTAAA TGACGAGAGG AGCAAGCCGT TGGCCAAATA CTGTGGTAAT 
AGTGTGCCAG AGGATCTGCT CAGCCATTCG CGTCAGCTTG TCCTCAAGTT CGTGTCGGAC 
TACAGCGAAT CGGATGGAGG CTTTGATTTA ACCTACACCT TCGAGGATCG AGCCAAGTGC 
GGCGGTCACA TTCATGCGTC CAGCGGTGAG CTAACCTCAC CGGAATATCC GGCCAACTAT 
TCGGCCGGCT TGGACTGCGA TTGGCATCTG ACCGGTACGA TTGATCATCT GCTGGAGATT 
CAGGTGGAGA ACTTTGAACT GGAGCAATCG CCGAACTGTT CAGCCGATTA TCTGGAAGTG 
AGAAATGGAG GCGGCACTGA TTCCCCACTG ATTGGGCGCT TCTGCGGACG GGATATACCC 
GCTCGGATAC CAGGCTTTAG CCACGAGATG AGGTTGATCC TTCATACGGA TTCGGCGATC 
AATGGTCGTG GATTCCGGCT GCGCTGGCGA ATCTTTGCCT TCGGATGCGG CGGAAGTTTG 
CGTTCGAATA TGGGCGCCAT TTCGTCGCCC AGATATCCGA ACTCGTATCC CAACATGGCG 
CACTGTGAGT GGCGAATTAG CTTGCATCCG GGATCGGGTA TTTCGCTTCT CATCGAGGAC 
TTGGAACTGG AGGGTTTGAG CAATTGTTAC TACGACAGCG TGAAGATCTA CACGGGCATT 
AAGCTGCCCA ATCAGAGCCC CTGCAAAGTA CTCTGCAAGG ACGATGATCT GCATAATCCG 
CTCATTCAGC TGGAGAACAA TAAGGGCACC ATTGTCTTCG ATTCTGATGC GTCCAACACA 
TTCCGTGGCT TTCGAATATC CTACAAGGCG AATTGCATTC GAAACCTTAC GGCCACGACG 
GGAACCATTG AGAGTCTGAA CTACATGGAA CCATTCTGGG AGACCATACC CATCAATTGT 
AGTTGGACTA TTCGTGCGCC CAAGGGCAAT CGCGTTCTAG TGGAAGTCTC ACATTTGGCA 
CGGCACGAAC AGCACGTCCC GACTGCCACC ATGCCCGGCG GATTGTACAT TGTGGACGGC 
AGGAATGTCC AGGAAATAGT CACACCACAA GCGATGAACA TCAGCGGCGA AGTGCTGACC 
GTCGTCCACA ATGCCAGCAA TGTGAACTTT CAGCTGGACT ATCGCATCGA TGGCTGCATG 
GAGGAGCTGC GCGGCACGTT CGGATTCTTT CAATCGCCCA ATTATCCAAA AATGTATCCG 
AACAACCTGG AGTGCTATTG GCTGATCACC GTCGAGCAGG ATAGCGCCAT CGAGCTTACG 
ATCAACAACA TAGATCTCGA GGACAGTCCC AATTGTACCA AGGATGCGCT GACGGTTTCC 
AATCATAAGA ATTCGGTGGA AGTCCACGAA CGACATTGCG GTTCCACGAC CAAATTGGTG 
ATCACCAGTT CGGGTCACAG GCTGCACGTG CGTTTCATCT CGGACAATTC GCACAATGGA 
CTCGGTTTTG AAGCCACCTA TAGAACTGTG AAAGCAACTT GTGGTGGCAA ATTGACGGCG 
CGAAACGGAG TGATCGAATC GCCAAATTAT CCGCTCAATT ATCCAGCTCA CAGTCGATGC 
GAGTGGCAGG TGGAGGTCTC CCAGCACCAC CAGATCGTTT TCGAGATGGC GGATCTTAAT 
CTTGAGTCGG GCTACGACTG CAACTGGGAT TATCTGGAGG CCTACGATCT GACGGAGGAC 
GACACGGAGG GTGAAAGATT GTTCAAGGTC TGCGGCGACG AGACGGAAGA TGACAAGCTG 
CTTTCTTCCT CATCCAATAT GGCTGTGGTG CGATTTATCA GCGATGATTC CGTTTCGAAA 
AAGGGCTTCC GGCTGCACTT CCACGAGTCC TGTGGCCAAA CGATAATCGT TGATGAAACA 
ATGTTTGATT ACATCCAAAT GTCCAGGCAG GCGGCCCGAA ACGAGAGCTG CTTATGGGTA 
TTCCAAGCCG TGGAGCCCAA CAAACGCATC ATCTTTACGC CCACCCATGT GAAGCTGCGC 
GAGGATGCCA ACCAGCAATA TCCCACGGAG GGTGATTGCC TCAATGTGGG AGTTAAGATC 
TACGAGGGTA CAGAGCCGCA GGGAACACCG CGTCTCAAGT TCTGTCGCTC GCATCCGCCG 
GCCTTGATTT CCAATGGCCA GGCTCTGACC GTCAGTGTGC CGCTCCAGCT GGTGGAGGAG 
TTCCAAGGAC ACTACATGAC CATGGACACG TCCTGCGGCA GTATTTACAA TGCTCTGTCC 
GGAAAGTTCA CTTCACCCTA TTACCCCGCC TCGTATCCGC CAAACATTGA ATGCCTGTGG 
CTGCTGGAAG CCAGCATGGG TAACTCCCTC AGTCTCACGC TGGAGTCGAT GGACTTGGAG 
AAGTCAGAGA GTTGCAATCG GGATTATCTG GAAGTGCGCG AGGAATCGGA GAGCGGCCAA 
CTGATCGGCG TTTACTGTGG CAACGAAGTG CCCGGCGTGA TTCACTCGCG CGGTGCCATC 
TGGATGAAGT TCAAGAGTGA CGACGACAAT GTGGGCGAGG GATTCATGGC CTCCTATAAC 
TACGAGCACC ACAACGAGCT GAATGGCACT GAAGGCACCA TTGAATCGCC GCACTTTCCA 
AGCAAATTCC AGGATCCGGT GCCGTACAGC TGGCGCATTA CGGTGGACAA GGAGTACGTG 
GTGGCGATAT CCCTGCTCTA TCTGAGGGAT CTGGACCAAC CGCATTTAAA TTTCTACGAC 
GGCTATTCGG ACATTGGAGC TCGTATCGAG GTGACTGATC CCGACGAAAC CATCATCTCC 
AGCACGAATG TGGTTTACTT CACGAGCAAT CGAGGTCCTT TCAAGTTAAA TTGGAATCGA 
TTATCCAAGG AGGCGCTACG TTCGAATCGT ACGGCAGAGG AGCGGACACG CCAGTGTGGC 
AATCAGTTGA TTACAATCGA TCGATCGGTG ATCGGATTTC ATTCGCCTGG CTATCCCAAT 
GGCTACGAGC AGGATCTCAA TTGCTTCTGG ACTTTGGTGC CCTCGAATCC GGCTATGCAT 
GCCGTACTCA CACTGTCCCA AATCGATCTG GAGATATTTA GCGAAGATTG CATTGCTGAC 
TATGTTAAGA TTTTTAGTGG GAGCGATCTG CAGAACTGGT CGGAGCTAAG AACGTTGTGC 
TCCCTGCCCA CGGAGTCGAG TGACCGAGTC TTTCATGGAA GGCCCTATCT CAGGGTGGAA 
TTCGTGACGG ATCCAAGTGT GAATAAGACG GGATTCAATG GCATTGTGCG CACGGCCTGC 
GGTTCGGAAA TCACTGCCTC CAAGGGACTG GTTAACATTA CGGAGATCCT GAAGGTCCTT 
CCGCGGCCAA ACCACGATTG TGTGTGGACC ATAAAAGTGC GCCAGGGCCG TAGAATCAAG 
ATCGACTTCC CCGACTTCCA GTTGCAGAAC AACATGGCCT CCGGATCGAG TGATTGCCGA 
AATTATCTTC TACTCCGCAA TGGAAACGAT GAAGATTCGC CCTTCTTGGG ACGTGGCAAG 
TACTGTGAGG ATGTCGTGCA CGAGGTCCTG AACACCTCCT CGAATAAGGC CTATATAAAG 
TTCCACTTTG CGAGTCCACC CCGCTTCCTG GTGTCCTTCC GCTTCGAGGA ACTGCGTTAC 
ACCGACTCTG GCCGTATCCG ATTGTCCGCC TCCGGAGATG AGCAGTTCAT TAGCTCACCG 
TATTACCCAC ATCTTCCGCA TCCCCATTCC GAGTGCATTT GGATCGTTGA AGCACCACCA 
GAACACCGCA TAATGCTGCA CTTTCAAGGC GCATTCGATA TGTTGGACGC CACCGGGGAA 
CCGGAGGAGT GCCAACGGGA ATTTGTTTTG ATCAACGATG GCAGTACGGA GCTAAGACCG 
GAGATCGGTC GCTATTGTGG CAATCGTAAG CCGGATACGA TCTACTCCAC CGGTAACCAG 
ATGAGGATTC GCTACTTCAC CGATGTCTCG GAGCCACATA TGGGCTTCAA TGCCAGTTTG 
AGTGTGGCCC GATGCGGAGG CTCCTTCCAT AGTCCCGAGG GCGTGATAGC CTCGCCGTCG 
AGGGACCTCC TGTTGATTCA CGAGGAAGGC AAGCAGCTGC AGGAATGCGT TTACACCATC 
GAACTGGAGA AGGGCAGCAC CATCGATCTG ACTAGCGAAT ATCTGCAGAT ACCCACGTTG 
CGGAATGGTA GCTGCTCGCA GCGAAATCAC TTGATGCTCG AAGAGATGGA TGCCTTTGGA 
TTGGACGGTG AGGAGAAGAT CGTGGACACC CTAATGCTCT GCGGTATGGA AGCCAAGCAC 
CTGATAAGTG AGACGAACAA GATCGTGTTC CGATATCGCT TCCTGGATGG CATTCCGGCG 
GAGAATCAGG GCTTCCGTTT AAAGTACACC TCGCTGGGCT CACGATGCGG CGAGACCATC 
TACGCATCCG TGGGAGTTCT CCAAACACCC GGTTATCCCT TAGGCGTACC CCATCCCATG 
CACTGCAAGT GGCAGGTGCA AGTGCCCAAG GGCAGAAGGG TGCGTCTGGA GATTCTCGAC 
TTTAACACCG GCACGAATAT GGATCTCAGA GGAAGGCTTG GATTCCGTGG CCGCCTTACC 
GTGGCCAACG ATTTCAAAAT GCAATCCATC CTGGGTCGCT ACAACGTCGA TCCTCCTGCG 
GAGGTGCTTT CCTCGGACAA TACAATGGGC ATCGATGCCT TCCTACTGCC CATTGTTCAG 
AATCATGGAA TCAAGCTGCG GTTCAGTGCC TATGGCTCCA GTAGCTGTCC GGGATTCACG 
GTAATGATGA ATGAGGTCGC AGATATTCAG TTCCAGCGGT TCAATATCAG CCGTCCGCTT 
CACTGCAGCT ATAAAGTCGT TCCACCGTCC AATAGTACCC TCCTGATTCG GGTGAAAGAG 
TACAACACCA CGTCCGTAAT GATGTGGAAT ACACATATGT GCGCATTGCT CTCGCCGCTT 
AAGTTTAACC GCTTGGAGCA GGAGGAGGAG CTCATGGAGC GCATTCTCTG CGATTACCAG 
TCACCCGCTC CTGGCAAGCC CCTGCCCTCC ATCCGGTTGC CTTTCCCCAT TCAACTGGTC 
GTCTCGGCAT CCGCCCGCAA CGCAATGACC AATCTCGTCC TGAGCTATAG CACACAGTCG 
TGCGGCGGAG TCATTATCCT GGAACCGGGT GATAATATGA CGGTGCACCA GCCATCGGGA 
ATGGTGTCGG CGGCGGGCGC CATTGACTGT GCCTGGGCCA TTGGACCCTA TACGGATGCC 
AGTGGCGAGG ACGAGGTCCT GGTGCCGCAG GACATTCAGC TGGAGGTGTC GGTATATAAC 
GTCAACCTGC CAGCACCGAG CCCGTCCGCA CAATCTCCGG AAGCCCCATG CCTGCATCAC 
TATCTTAAGG TATACAATGG ACCCGATCAG AACTCACCAT CGTTGGGACT GTTTTGCAAT 
CAGGCCACAG CTGTGAACAT GGTGGTGGAG CGAGGTCTCT TCCTGGAATA TCACTCCGAT 
AGTTTCTCAG CCAATGCCAC CTTCAATGTG TCCATCAAGT ATGGATCTGG ATGCGGTGGC 
AAGTTGGTAT ATCCCTATAG AGCCATCGAC TTTGCCGAGC AATACAAGAA CAATGTGGAG 
TGCATCTGGG AGGTCGAGGC GACAATGGGC TACCACATCG GTCTAACATT CCAAGGACGC 
TTTTATATCG AGGATAGCCC CGGTTGCACA AAGGATTACC TGCTCGTCCA GCAGCGCAAC 
GAGACCACTG GCAATTGGAC CGACCTGCAG AGGATTTGCG GTCGTGTTGC GCCGGAGATG 
ATAAACACAA CTTCACCGTA TCTCCGGCTA ATCTTCCGAT CCGATGGCGA TGTGGTGGCC 
GATGGTTTTT TGGCCAAATT CGAAAGGAAT TGCGGTGGCC TGCTGTACGC CGACAGCACG 
GAACAGGAGC TGGCCAGTCC GGGCTTTCCG AATGGCTATG AAAAGTACTT GCAATGTAAT 
TGGACGATAG TGCCCAGGAG TCCATCGATG GGCGGTGTCC TGGTCAGCTT TGTCAATTTC 
GATCTGGAAC AGGGACCCAT ATCCGTTTGC CTCTACGACA ATCTCACGGT GACCACAAAG 
GACAAGGGCA AGGATCCGCA ACAGACCACT CTCTGCGGCG TAAAGCATAA CCACGAGTAT 
CGGGGCAAGG AATATGTTAA TCTACTGCTC CGAACGGATG GCAGTTATTC CGGTCGCGGA 
TTCACATTAC TCTATACCAG CCGCCTTTGC GGTGGAATTA TCTCGCGAAC ATCAATGGTG 
GAATCACCCG TACAGCATAC GGATAACACC CTGCCACCTG GCAGTGATTG CTATTGGAAT 
CTAACCGCCC CGGCTGGTTA CAAGTTCAAC ATTAAGTTCC TATTCATTGA CTTTGAGGCG 
AATAGCAATT GTGCCTACGA TGGCGTCGAG GTATTCTCCG GACCCATCCC GGACGAGCGA 
TACCGATGGG GACGCTTCTG CGGTCGCATT AACGAGGATC TGCCACTGAT CAGTATTCCC 
CAAGAGCGGG GCATCATACA CAGCTTCTCG GATGATCGGG ATCCATCGAG GGGATTCCGT 
GCCTTGGTGC GCGTAATGCC AAATTGCGAT GAGAAGATCT CGCTGAACGG ATCGTCGCGT 
TATGTCTATA GCAAATTTAA CAACGCTGGC GGTTATCAAA ACGATTTGGA CTGTCAAATA 
GTATTCCGGG TGAATCCTGA TCAGCAGATA AGCGTGGAGT TCAGTAATTT CCATGTCCAA 
GACACGGACG GATGCCGCAG TGATTATGTG GAGTTGCGCG ATGGTGGCGG AACCTTTGCC 
GACATTATCG GACGTTTTTG TGGCCAGAAT CAACCGCCCA CCTTGAGAAC AACCAGGCAT 
ACGCTCTATA TGCGCTTTGT CACCGATAAT AAGGTGACGG ATACGGGTTT CCAGGTGACC 
ATCAATGCTA TTCCCCGACT ATGCGGCAGT TCAGAGATTA CGTTGAGTGC GGATGGCACC 
AAGGAGGTCA CCATTAATTC TCCGGCAAGG ACGCCAGGTG GCAATTATCC CAATGGAGTG 
TCGTGCTTCT GGAAAATTAA GGGCGATTCC CTGCTGAGGG TGCAGTTCGT CAACTTTGAT 
CTCCACGGAC CGAATCAAAA TGGTAGCTGC GTGGATGACT ATTTGAAAAT CTACAATAGT 
GAGGATGCAC CGCTGTTGGA GCAGGGATTG GGCACCGACC TGGTATTCAA TGGCCAGACC 
TCCAGTAAGA ACGGTTTCGG CTTTGCCACG GAGCATGTGT ACTGCGGCAA TGTGAAGCCG 
GACATCTATT ATGGCCGGTC CAGCGAGGTG TATCTGAAGT TCCGGTCAAA GGGATTGGAG 
CAGCATGGTG GATTTCAGTT ACAGGTCGCT CTGAACTCCA ACCGAGAGCG CCACTACGAT 
GGACTGCAGG GCAGAGTGCA TTTATCACAG TCGGCCGATT GTAACATCAT TATTCGGGCG 
CCACCAAATT ACACCTTAAG TTTGTACTAC ACCGAATTGA TATTCGGCAC CTACGACTGC 
GAAATGGAGA ATTTGGAGGT GTTCGACAGG ACGAATCGAT CGCTGCAGCG CGTCTGCTCC 
TTTGTGGACA TGGGCAAGAG CCTGTTCAGC AATGCGAATG AACTGCGGCT ACAGATGAAG 
ACTGGCTCGT ACTTGACCTC GCTGGATCTC ACCTATTTGG CCAGTCCGGT GGAGAAGGGT 
CCTGGTTGCG GTGGTCAATT CTACAATACC GAGGGCATAT TCTCAAATCC CTTCTATCCG 
AACAATGTGC GCAATAACTC AGAGTGTCAG TGGATTGTCC GCGTGCCGAG CAACAATGTT 
GTGTTCCTTA CATTTGAAGT CTTCAATCTG GGCTCGAAGA CCACCTGTCA CACGGACTAC 
CTGCAAATCC TGGAACAGGA CGCAACCGGT GAGGAGCGCG AAATGAGACG CTTCTGTGGC 
GAGGATAACC CAAAGTACTA CAAGAGCCGG CGCAGCCAGG TGCTCGTCCG CTTTCACAAG 
ACCGTCAACT ACGATGGTAT CGGTTGGGTG ATCCGCTTTG CCGGTGTCTA CTCCGACCAT 
CAGATACCCA GACACCTGTT GGGCGGTTCT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_727348.2
CDS	1..11253
Translation	MEGAARSRLLLCWTLLAIITDTWPIAEGFVNSPKIISKDGNLIFESGANRNISFRLSGNSRLTINEELDVMELLLATSGSKKRSGGKDDDFVDARELADQLADFNRRAFGANGLSAMLRVQQNRTRGSMALLRRFQTRLRALENRVDRMKTDLEANSCASGPCENGGTCYNTYTGFRCQCRSAFEGTKCEMDVNECALYEGTDLGCQNGGQCQNHFGTYSCLCQPGWHGMHCTQRKADCSQSSAWELCGHGSCVPSADDAGYRCICEPGWKTNGLTPICGEDVDECSDSAAHKPCSTSCINLPGSFTCAPCPAGLTGNGVSCRDLDECQTNNGGCSLSPKVDCINTYGSYHCGECPVGWTGDGRKCERSPQDIDIPAGQTPRTCPAGNNPCYPTASCFLISGTTSCRCPMGMVGTGYGPNGCVNGTTTNCKENPCLNGGICLFAGPSNYTCLCPIGFRPPICEPQPSPCDQHPCKNGGRCRPTTSGDLFVCQCLPGYRGRLCETRFSSCNGMLSAQSGRLRYPPEGTGYEHNAQCAWVIRTNESLVVNVTFNSFDVEDSTECRFDWLQINDGRSAAAQIIGRYCGNHLPHGGNIVSSGNQLYLWFRSDNSTAKEGFDLTWNSMEPQCGGRLNFETHGTLASPGSPGNYPKNRDCRWQLVAPTTKRIKLTFFSLQLEQHANCNFDYVLIKDSISGRELAKYCTTGAPAPLLLPTHLAEIHFHSDAEGSDTGFQLHYSVEERVPGCGGVYTAKEGTISESSTANTEPGGVSCEYEIHLAVGEQVVIQFARLELDPLDCLEVLDITDEGGSILQEKICGSDASRLNPPTFTSEFNRLKIKFYARAGSFQLNYRMACDYKLNNEQGTITSPGYPNLTRSDRICTYTISTATNTVISLKRIDFQLTNGESDDDDNDECLTTNLRINDGLNRKILGPYCGKNQPEENFVSETNYLQLHLSTDVDSMGRGFKFEYRALATGNDKCGGVHTRSGDHIRLPVHDDSYAGEATCYWVIMAPANKAIRLHWNSFSLENAVDCIYDYLEIYDSLGAQVNDERSKPLAKYCGNSVPEDLLSHSRQLVLKFVSDYSESDGGFDLTYTFEDRAKCGGHIHASSGELTSPEYPANYSAGLDCDWHLTGTIDHLLEIQVENFELEQSPNCSADYLEVRNGGGTDSPLIGRFCGRDIPARIPGFSHEMRLILHTDSAINGRGFRLRWRIFAFGCGGSLRSNMGAISSPRYPNSYPNMAHCEWRISLHPGSGISLLIEDLELEGLSNCYYDSVKIYTGIKLPNQSPCKVLCKDDDLHNPLIQLENNKGTIVFDSDASNTFRGFRISYKANCIRNLTATTGTIESLNYMEPFWETIPINCSWTIRAPKGNRVLVEVSHLARHEQHVPTATMPGGLYIVDGRNVQEIVTPQAMNISGEVLTVVHNASNVNFQLDYRIDGCMEELRGTFGFFQSPNYPKMYPNNLECYWLITVEQDSAIELTINNIDLEDSPNCTKDALTVSNHKNSVEVHERHCGSTTKLVITSSGHRLHVRFISDNSHNGLGFEATYRTVKATCGGKLTARNGVIESPNYPLNYPAHSRCEWQVEVSQHHQIVFEMADLNLESGYDCNWDYLEAYDLTEDDTEGERLFKVCGDETEDDKLLSSSSNMAVVRFISDDSVSKKGFRLHFHESCGQTIIVDETMFDYIQMSRQAARNESCLWVFQAVEPNKRIIFTPTHVKLREDANQQYPTEGDCLNVGVKIYEGTEPQGTPRLKFCRSHPPALISNGQALTVSVPLQLVEEFQGHYMTMDTSCGSIYNALSGKFTSPYYPASYPPNIECLWLLEASMGNSLSLTLESMDLEKSESCNRDYLEVREESESGQLIGVYCGNEVPGVIHSRGAIWMKFKSDDDNVGEGFMASYNYEHHNELNGTEGTIESPHFPSKFQDPVPYSWRITVDKEYVVAISLLYLRDLDQPHLNFYDGYSDIGARIEVTDPDETIISSTNVVYFTSNRGPFKLNWNRLSKEALRSNRTAEERTRQCGNQLITIDRSVIGFHSPGYPNGYEQDLNCFWTLVPSNPAMHAVLTLSQIDLEIFSEDCIADYVKIFSGSDLQNWSELRTLCSLPTESSDRVFHGRPYLRVEFVTDPSVNKTGFNGIVRTACGSEITASKGLVNITEILKVLPRPNHDCVWTIKVRQGRRIKIDFPDFQLQNNMASGSSDCRNYLLLRNGNDEDSPFLGRGKYCEDVVHEVLNTSSNKAYIKFHFASPPRFLVSFRFEELRYTDSGRIRLSASGDEQFISSPYYPHLPHPHSECIWIVEAPPEHRIMLHFQGAFDMLDATGEPEECQREFVLINDGSTELRPEIGRYCGNRKPDTIYSTGNQMRIRYFTDVSEPHMGFNASLSVARCGGSFHSPEGVIASPSRDLLLIHEEGKQLQECVYTIELEKGSTIDLTSEYLQIPTLRNGSCSQRNHLMLEEMDAFGLDGEEKIVDTLMLCGMEAKHLISETNKIVFRYRFLDGIPAENQGFRLKYTSLGSRCGETIYASVGVLQTPGYPLGVPHPMHCKWQVQVPKGRRVRLEILDFNTGTNMDLRGRLGFRGRLTVANDFKMQSILGRYNVDPPAEVLSSDNTMGIDAFLLPIVQNHGIKLRFSAYGSSSCPGFTVMMNEVADIQFQRFNISRPLHCSYKVVPPSNSTLLIRVKEYNTTSVMMWNTHMCALLSPLKFNRLEQEEELMERILCDYQSPAPGKPLPSIRLPFPIQLVVSASARNAMTNLVLSYSTQSCGGVIILEPGDNMTVHQPSGMVSAAGAIDCAWAIGPYTDASGEDEVLVPQDIQLEVSVYNVNLPAPSPSAQSPEAPCLHHYLKVYNGPDQNSPSLGLFCNQATAVNMVVERGLFLEYHSDSFSANATFNVSIKYGSGCGGKLVYPYRAIDFAEQYKNNVECIWEVEATMGYHIGLTFQGRFYIEDSPGCTKDYLLVQQRNETTGNWTDLQRICGRVAPEMINTTSPYLRLIFRSDGDVVADGFLAKFERNCGGLLYADSTEQELASPGFPNGYEKYLQCNWTIVPRSPSMGGVLVSFVNFDLEQGPISVCLYDNLTVTTKDKGKDPQQTTLCGVKHNHEYRGKEYVNLLLRTDGSYSGRGFTLLYTSRLCGGIISRTSMVESPVQHTDNTLPPGSDCYWNLTAPAGYKFNIKFLFIDFEANSNCAYDGVEVFSGPIPDERYRWGRFCGRINEDLPLISIPQERGIIHSFSDDRDPSRGFRALVRVMPNCDEKISLNGSSRYVYSKFNNAGGYQNDLDCQIVFRVNPDQQISVEFSNFHVQDTDGCRSDYVELRDGGGTFADIIGRFCGQNQPPTLRTTRHTLYMRFVTDNKVTDTGFQVTINAIPRLCGSSEITLSADGTKEVTINSPARTPGGNYPNGVSCFWKIKGDSLLRVQFVNFDLHGPNQNGSCVDDYLKIYNSEDAPLLEQGLGTDLVFNGQTSSKNGFGFATEHVYCGNVKPDIYYGRSSEVYLKFRSKGLEQHGGFQLQVALNSNRERHYDGLQGRVHLSQSADCNIIIRAPPNYTLSLYYTELIFGTYDCEMENLEVFDRTNRSLQRVCSFVDMGKSLFSNANELRLQMKTGSYLTSLDLTYLASPVEKGPGCGGQFYNTEGIFSNPFYPNNVRNNSECQWIVRVPSNNVVFLTFEVFNLGSKTTCHTDYLQILEQDATGEEREMRRFCGEDNPKYYKSRRSQVLVRFHKTVNYDGIGWVIRFAGVYSDHQIPRHLLGGS

Target ORF information:

RefSeq Version	NM_167193.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster cubilin (Cubn), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_167193.2

ORF Insert Sequence:

ATGGAAGGAG CTGCGAGGAG CAGACTCCTA TTGTGTTGGA CACTCCTTGC CATCATCACA 
GATACTTGGC CCATTGCCGA GGGTTTTGTC AACTCGCCAA AAATTATAAG CAAAGATGGC 
AATTTGATTT TTGAATCCGG TGCAAATCGC AATATAAGCT TTCGATTGAG CGGAAATTCG 
CGGCTTACCA TCAACGAGGA ATTGGATGTT ATGGAGCTCT TGCTGGCCAC CAGTGGCTCC 
AAAAAGCGTT CCGGTGGCAA GGATGATGAT TTCGTAGATG CGCGCGAGCT GGCGGATCAG 
TTGGCTGATT TCAATAGGCG CGCCTTTGGA GCGAATGGCT TGAGTGCAAT GCTGCGAGTG 
CAGCAAAATA GGACCCGAGG ATCGATGGCT CTGTTGCGGC GTTTTCAGAC ACGACTCAGA 
GCGCTGGAGA ATCGGGTGGA TCGAATGAAG ACCGATTTGG AGGCAAACAG CTGTGCAAGT 
GGACCCTGCG AGAATGGAGG CACCTGCTAC AACACATACA CCGGATTCCG TTGCCAATGT 
CGATCGGCAT TCGAGGGTAC CAAGTGCGAG ATGGATGTCA ACGAGTGCGC ACTGTACGAG 
GGCACCGATC TGGGTTGCCA AAATGGTGGA CAATGCCAGA ATCATTTCGG CACATACAGT 
TGCCTTTGCC AGCCGGGTTG GCATGGGATG CACTGCACCC AGCGCAAGGC GGATTGCTCA 
CAGTCCAGTG CATGGGAGCT GTGTGGCCAT GGATCTTGTG TGCCCAGTGC AGACGATGCC 
GGCTATCGAT GCATCTGCGA ACCGGGTTGG AAGACGAATG GCTTGACACC CATTTGTGGC 
GAGGATGTGG ACGAATGCAG CGATTCGGCC GCCCACAAGC CCTGCTCCAC CAGCTGCATT 
AATCTGCCTG GCAGCTTCAC CTGTGCTCCC TGTCCAGCCG GCTTGACCGG GAATGGGGTC 
AGTTGCCGGG ACTTGGACGA ATGCCAGACG AACAACGGTG GATGCAGTCT TAGTCCCAAG 
GTAGATTGCA TAAATACCTA TGGTTCATAT CATTGCGGCG AGTGTCCCGT TGGCTGGACC 
GGCGATGGGC GGAAGTGCGA GAGAAGCCCC CAGGACATTG ACATCCCAGC TGGACAGACG 
CCAAGAACTT GTCCGGCGGG CAACAATCCC TGCTATCCGA CGGCGAGCTG CTTTTTGATC 
TCCGGCACCA CATCCTGCAG ATGTCCAATG GGCATGGTGG GAACAGGATA CGGTCCGAAT 
GGCTGTGTCA ATGGCACGAC CACAAACTGC AAGGAAAATC CCTGCCTGAA TGGTGGCATT 
TGCCTGTTCG CCGGACCCTC GAATTATACC TGCCTGTGTC CAATTGGCTT CCGTCCGCCC 
ATCTGTGAGC CACAGCCGAG TCCTTGCGAT CAGCATCCAT GCAAGAATGG AGGACGTTGC 
CGTCCCACCA CCAGTGGGGA TCTATTCGTC TGCCAGTGTT TACCTGGCTA CCGGGGACGA 
CTTTGCGAGA CGCGCTTCAG CAGCTGCAAT GGAATGCTGA GTGCCCAGAG TGGCAGGCTT 
AGGTATCCGC CGGAGGGCAC TGGATACGAG CACAATGCCC AGTGCGCTTG GGTTATTCGC 
ACCAACGAAT CGCTGGTGGT AAATGTCACG TTCAATAGCT TCGATGTGGA GGATTCCACG 
GAATGCCGCT TCGATTGGCT GCAGATCAAC GATGGCCGTT CGGCGGCTGC CCAGATAATT 
GGACGCTATT GTGGTAACCA TTTACCGCAT GGCGGGAATA TCGTATCATC CGGCAATCAA 
CTCTATCTAT GGTTCCGATC CGATAACTCG ACGGCTAAAG AGGGATTCGA TCTCACATGG 
AACTCGATGG AGCCGCAGTG TGGCGGTCGC TTGAATTTCG AGACACATGG CACACTTGCG 
TCGCCGGGAT CGCCCGGAAA TTATCCGAAG AATCGGGACT GCCGGTGGCA ACTGGTGGCG 
CCCACCACCA AACGCATCAA GCTGACCTTC TTCAGTTTGC AGCTGGAGCA GCACGCAAAT 
TGCAATTTCG ATTATGTCCT GATCAAGGAC AGCATTTCCG GACGTGAGCT GGCCAAATAT 
TGCACCACGG GAGCGCCAGC ACCGCTGCTC TTGCCCACCC ACCTGGCCGA GATCCACTTC 
CATTCGGATG CCGAGGGCAG CGATACTGGC TTCCAGCTGC ACTATTCCGT GGAGGAGCGT 
GTGCCCGGCT GCGGTGGTGT CTACACCGCC AAGGAGGGCA CCATTTCTGA ATCATCCACA 
GCCAATACCG AACCAGGTGG TGTCTCCTGC GAATACGAAA TCCATTTGGC CGTGGGCGAG 
CAGGTTGTCA TTCAGTTTGC GCGTCTGGAG CTGGATCCAC TTGACTGCCT GGAGGTGCTG 
GATATCACGG ACGAGGGTGG TAGCATTTTG CAGGAGAAGA TTTGCGGATC GGATGCGTCG 
CGTTTGAATC CTCCTACCTT CACTTCGGAA TTCAATCGGC TGAAGATTAA GTTTTATGCC 
CGTGCCGGCA GCTTCCAGTT GAACTACCGG ATGGCCTGCG ATTATAAACT GAATAATGAG 
CAGGGCACCA TTACCTCACC CGGTTATCCC AATCTAACAA GATCGGATCG TATCTGCACC 
TATACGATTA GTACGGCCAC CAATACGGTG ATTAGTCTGA AGAGGATCGA CTTTCAGTTG 
ACCAATGGCG AGAGCGACGA TGATGATAAC GATGAATGTC TGACAACCAA CTTGAGAATC 
AACGATGGAT TAAACCGAAA GATCCTGGGC CCCTATTGCG GCAAGAATCA GCCGGAGGAG 
AACTTTGTCA GCGAAACCAA CTACCTGCAG TTGCACCTGT CCACCGATGT GGACAGCATG 
GGGCGTGGCT TTAAGTTCGA GTATCGAGCC CTGGCCACCG GCAACGATAA GTGCGGTGGC 
GTTCATACCC GTTCCGGCGA TCATATCCGC TTGCCGGTGC ACGACGACTC ATATGCCGGC 
GAAGCCACCT GTTACTGGGT GATAATGGCG CCGGCAAACA AGGCCATCCG CCTCCACTGG 
AACAGCTTCA GTTTGGAAAA TGCGGTGGAC TGCATCTATG ACTATTTGGA GATCTATGAT 
AGTCTGGGTG CCCAGGTAAA TGACGAGAGG AGCAAGCCGT TGGCCAAATA CTGTGGTAAT 
AGTGTGCCAG AGGATCTGCT CAGCCATTCG CGTCAGCTTG TCCTCAAGTT CGTGTCGGAC 
TACAGCGAAT CGGATGGAGG CTTTGATTTA ACCTACACCT TCGAGGATCG AGCCAAGTGC 
GGCGGTCACA TTCATGCGTC CAGCGGTGAG CTAACCTCAC CGGAATATCC GGCCAACTAT 
TCGGCCGGCT TGGACTGCGA TTGGCATCTG ACCGGTACGA TTGATCATCT GCTGGAGATT 
CAGGTGGAGA ACTTTGAACT GGAGCAATCG CCGAACTGTT CAGCCGATTA TCTGGAAGTG 
AGAAATGGAG GCGGCACTGA TTCCCCACTG ATTGGGCGCT TCTGCGGACG GGATATACCC 
GCTCGGATAC CAGGCTTTAG CCACGAGATG AGGTTGATCC TTCATACGGA TTCGGCGATC 
AATGGTCGTG GATTCCGGCT GCGCTGGCGA ATCTTTGCCT TCGGATGCGG CGGAAGTTTG 
CGTTCGAATA TGGGCGCCAT TTCGTCGCCC AGATATCCGA ACTCGTATCC CAACATGGCG 
CACTGTGAGT GGCGAATTAG CTTGCATCCG GGATCGGGTA TTTCGCTTCT CATCGAGGAC 
TTGGAACTGG AGGGTTTGAG CAATTGTTAC TACGACAGCG TGAAGATCTA CACGGGCATT 
AAGCTGCCCA ATCAGAGCCC CTGCAAAGTA CTCTGCAAGG ACGATGATCT GCATAATCCG 
CTCATTCAGC TGGAGAACAA TAAGGGCACC ATTGTCTTCG ATTCTGATGC GTCCAACACA 
TTCCGTGGCT TTCGAATATC CTACAAGGCG AATTGCATTC GAAACCTTAC GGCCACGACG 
GGAACCATTG AGAGTCTGAA CTACATGGAA CCATTCTGGG AGACCATACC CATCAATTGT 
AGTTGGACTA TTCGTGCGCC CAAGGGCAAT CGCGTTCTAG TGGAAGTCTC ACATTTGGCA 
CGGCACGAAC AGCACGTCCC GACTGCCACC ATGCCCGGCG GATTGTACAT TGTGGACGGC 
AGGAATGTCC AGGAAATAGT CACACCACAA GCGATGAACA TCAGCGGCGA AGTGCTGACC 
GTCGTCCACA ATGCCAGCAA TGTGAACTTT CAGCTGGACT ATCGCATCGA TGGCTGCATG 
GAGGAGCTGC GCGGCACGTT CGGATTCTTT CAATCGCCCA ATTATCCAAA AATGTATCCG 
AACAACCTGG AGTGCTATTG GCTGATCACC GTCGAGCAGG ATAGCGCCAT CGAGCTTACG 
ATCAACAACA TAGATCTCGA GGACAGTCCC AATTGTACCA AGGATGCGCT GACGGTTTCC 
AATCATAAGA ATTCGGTGGA AGTCCACGAA CGACATTGCG GTTCCACGAC CAAATTGGTG 
ATCACCAGTT CGGGTCACAG GCTGCACGTG CGTTTCATCT CGGACAATTC GCACAATGGA 
CTCGGTTTTG AAGCCACCTA TAGAACTGTG AAAGCAACTT GTGGTGGCAA ATTGACGGCG 
CGAAACGGAG TGATCGAATC GCCAAATTAT CCGCTCAATT ATCCAGCTCA CAGTCGATGC 
GAGTGGCAGG TGGAGGTCTC CCAGCACCAC CAGATCGTTT TCGAGATGGC GGATCTTAAT 
CTTGAGTCGG GCTACGACTG CAACTGGGAT TATCTGGAGG CCTACGATCT GACGGAGGAC 
GACACGGAGG GTGAAAGATT GTTCAAGGTC TGCGGCGACG AGACGGAAGA TGACAAGCTG 
CTTTCTTCCT CATCCAATAT GGCTGTGGTG CGATTTATCA GCGATGATTC CGTTTCGAAA 
AAGGGCTTCC GGCTGCACTT CCACGAGTCC TGTGGCCAAA CGATAATCGT TGATGAAACA 
ATGTTTGATT ACATCCAAAT GTCCAGGCAG GCGGCCCGAA ACGAGAGCTG CTTATGGGTA 
TTCCAAGCCG TGGAGCCCAA CAAACGCATC ATCTTTACGC CCACCCATGT GAAGCTGCGC 
GAGGATGCCA ACCAGCAATA TCCCACGGAG GGTGATTGCC TCAATGTGGG AGTTAAGATC 
TACGAGGGTA CAGAGCCGCA GGGAACACCG CGTCTCAAGT TCTGTCGCTC GCATCCGCCG 
GCCTTGATTT CCAATGGCCA GGCTCTGACC GTCAGTGTGC CGCTCCAGCT GGTGGAGGAG 
TTCCAAGGAC ACTACATGAC CATGGACACG TCCTGCGGCA GTATTTACAA TGCTCTGTCC 
GGAAAGTTCA CTTCACCCTA TTACCCCGCC TCGTATCCGC CAAACATTGA ATGCCTGTGG 
CTGCTGGAAG CCAGCATGGG TAACTCCCTC AGTCTCACGC TGGAGTCGAT GGACTTGGAG 
AAGTCAGAGA GTTGCAATCG GGATTATCTG GAAGTGCGCG AGGAATCGGA GAGCGGCCAA 
CTGATCGGCG TTTACTGTGG CAACGAAGTG CCCGGCGTGA TTCACTCGCG CGGTGCCATC 
TGGATGAAGT TCAAGAGTGA CGACGACAAT GTGGGCGAGG GATTCATGGC CTCCTATAAC 
TACGAGCACC ACAACGAGCT GAATGGCACT GAAGGCACCA TTGAATCGCC GCACTTTCCA 
AGCAAATTCC AGGATCCGGT GCCGTACAGC TGGCGCATTA CGGTGGACAA GGAGTACGTG 
GTGGCGATAT CCCTGCTCTA TCTGAGGGAT CTGGACCAAC CGCATTTAAA TTTCTACGAC 
GGCTATTCGG ACATTGGAGC TCGTATCGAG GTGACTGATC CCGACGAAAC CATCATCTCC 
AGCACGAATG TGGTTTACTT CACGAGCAAT CGAGGTCCTT TCAAGTTAAA TTGGAATCGA 
TTATCCAAGG AGGCGCTACG TTCGAATCGT ACGGCAGAGG AGCGGACACG CCAGTGTGGC 
AATCAGTTGA TTACAATCGA TCGATCGGTG ATCGGATTTC ATTCGCCTGG CTATCCCAAT 
GGCTACGAGC AGGATCTCAA TTGCTTCTGG ACTTTGGTGC CCTCGAATCC GGCTATGCAT 
GCCGTACTCA CACTGTCCCA AATCGATCTG GAGATATTTA GCGAAGATTG CATTGCTGAC 
TATGTTAAGA TTTTTAGTGG GAGCGATCTG CAGAACTGGT CGGAGCTAAG AACGTTGTGC 
TCCCTGCCCA CGGAGTCGAG TGACCGAGTC TTTCATGGAA GGCCCTATCT CAGGGTGGAA 
TTCGTGACGG ATCCAAGTGT GAATAAGACG GGATTCAATG GCATTGTGCG CACGGCCTGC 
GGTTCGGAAA TCACTGCCTC CAAGGGACTG GTTAACATTA CGGAGATCCT GAAGGTCCTT 
CCGCGGCCAA ACCACGATTG TGTGTGGACC ATAAAAGTGC GCCAGGGCCG TAGAATCAAG 
ATCGACTTCC CCGACTTCCA GTTGCAGAAC AACATGGCCT CCGGATCGAG TGATTGCCGA 
AATTATCTTC TACTCCGCAA TGGAAACGAT GAAGATTCGC CCTTCTTGGG ACGTGGCAAG 
TACTGTGAGG ATGTCGTGCA CGAGGTCCTG AACACCTCCT CGAATAAGGC CTATATAAAG 
TTCCACTTTG CGAGTCCACC CCGCTTCCTG GTGTCCTTCC GCTTCGAGGA ACTGCGTTAC 
ACCGACTCTG GCCGTATCCG ATTGTCCGCC TCCGGAGATG AGCAGTTCAT TAGCTCACCG 
TATTACCCAC ATCTTCCGCA TCCCCATTCC GAGTGCATTT GGATCGTTGA AGCACCACCA 
GAACACCGCA TAATGCTGCA CTTTCAAGGC GCATTCGATA TGTTGGACGC CACCGGGGAA 
CCGGAGGAGT GCCAACGGGA ATTTGTTTTG ATCAACGATG GCAGTACGGA GCTAAGACCG 
GAGATCGGTC GCTATTGTGG CAATCGTAAG CCGGATACGA TCTACTCCAC CGGTAACCAG 
ATGAGGATTC GCTACTTCAC CGATGTCTCG GAGCCACATA TGGGCTTCAA TGCCAGTTTG 
AGTGTGGCCC GATGCGGAGG CTCCTTCCAT AGTCCCGAGG GCGTGATAGC CTCGCCGTCG 
AGGGACCTCC TGTTGATTCA CGAGGAAGGC AAGCAGCTGC AGGAATGCGT TTACACCATC 
GAACTGGAGA AGGGCAGCAC CATCGATCTG ACTAGCGAAT ATCTGCAGAT ACCCACGTTG 
CGGAATGGTA GCTGCTCGCA GCGAAATCAC TTGATGCTCG AAGAGATGGA TGCCTTTGGA 
TTGGACGGTG AGGAGAAGAT CGTGGACACC CTAATGCTCT GCGGTATGGA AGCCAAGCAC 
CTGATAAGTG AGACGAACAA GATCGTGTTC CGATATCGCT TCCTGGATGG CATTCCGGCG 
GAGAATCAGG GCTTCCGTTT AAAGTACACC TCGCTGGGCT CACGATGCGG CGAGACCATC 
TACGCATCCG TGGGAGTTCT CCAAACACCC GGTTATCCCT TAGGCGTACC CCATCCCATG 
CACTGCAAGT GGCAGGTGCA AGTGCCCAAG GGCAGAAGGG TGCGTCTGGA GATTCTCGAC 
TTTAACACCG GCACGAATAT GGATCTCAGA GGAAGGCTTG GATTCCGTGG CCGCCTTACC 
GTGGCCAACG ATTTCAAAAT GCAATCCATC CTGGGTCGCT ACAACGTCGA TCCTCCTGCG 
GAGGTGCTTT CCTCGGACAA TACAATGGGC ATCGATGCCT TCCTACTGCC CATTGTTCAG 
AATCATGGAA TCAAGCTGCG GTTCAGTGCC TATGGCTCCA GTAGCTGTCC GGGATTCACG 
GTAATGATGA ATGAGGTCGC AGATATTCAG TTCCAGCGGT TCAATATCAG CCGTCCGCTT 
CACTGCAGCT ATAAAGTCGT TCCACCGTCC AATAGTACCC TCCTGATTCG GGTGAAAGAG 
TACAACACCA CGTCCGTAAT GATGTGGAAT ACACATATGT GCGCATTGCT CTCGCCGCTT 
AAGTTTAACC GCTTGGAGCA GGAGGAGGAG CTCATGGAGC GCATTCTCTG CGATTACCAG 
TCACCCGCTC CTGGCAAGCC CCTGCCCTCC ATCCGGTTGC CTTTCCCCAT TCAACTGGTC 
GTCTCGGCAT CCGCCCGCAA CGCAATGACC AATCTCGTCC TGAGCTATAG CACACAGTCG 
TGCGGCGGAG TCATTATCCT GGAACCGGGT GATAATATGA CGGTGCACCA GCCATCGGGA 
ATGGTGTCGG CGGCGGGCGC CATTGACTGT GCCTGGGCCA TTGGACCCTA TACGGATGCC 
AGTGGCGAGG ACGAGGTCCT GGTGCCGCAG GACATTCAGC TGGAGGTGTC GGTATATAAC 
GTCAACCTGC CAGCACCGAG CCCGTCCGCA CAATCTCCGG AAGCCCCATG CCTGCATCAC 
TATCTTAAGG TATACAATGG ACCCGATCAG AACTCACCAT CGTTGGGACT GTTTTGCAAT 
CAGGCCACAG CTGTGAACAT GGTGGTGGAG CGAGGTCTCT TCCTGGAATA TCACTCCGAT 
AGTTTCTCAG CCAATGCCAC CTTCAATGTG TCCATCAAGT ATGGATCTGG ATGCGGTGGC 
AAGTTGGTAT ATCCCTATAG AGCCATCGAC TTTGCCGAGC AATACAAGAA CAATGTGGAG 
TGCATCTGGG AGGTCGAGGC GACAATGGGC TACCACATCG GTCTAACATT CCAAGGACGC 
TTTTATATCG AGGATAGCCC CGGTTGCACA AAGGATTACC TGCTCGTCCA GCAGCGCAAC 
GAGACCACTG GCAATTGGAC CGACCTGCAG AGGATTTGCG GTCGTGTTGC GCCGGAGATG 
ATAAACACAA CTTCACCGTA TCTCCGGCTA ATCTTCCGAT CCGATGGCGA TGTGGTGGCC 
GATGGTTTTT TGGCCAAATT CGAAAGGAAT TGCGGTGGCC TGCTGTACGC CGACAGCACG 
GAACAGGAGC TGGCCAGTCC GGGCTTTCCG AATGGCTATG AAAAGTACTT GCAATGTAAT 
TGGACGATAG TGCCCAGGAG TCCATCGATG GGCGGTGTCC TGGTCAGCTT TGTCAATTTC 
GATCTGGAAC AGGGACCCAT ATCCGTTTGC CTCTACGACA ATCTCACGGT GACCACAAAG 
GACAAGGGCA AGGATCCGCA ACAGACCACT CTCTGCGGCG TAAAGCATAA CCACGAGTAT 
CGGGGCAAGG AATATGTTAA TCTACTGCTC CGAACGGATG GCAGTTATTC CGGTCGCGGA 
TTCACATTAC TCTATACCAG CCGCCTTTGC GGTGGAATTA TCTCGCGAAC ATCAATGGTG 
GAATCACCCG TACAGCATAC GGATAACACC CTGCCACCTG GCAGTGATTG CTATTGGAAT 
CTAACCGCCC CGGCTGGTTA CAAGTTCAAC ATTAAGTTCC TATTCATTGA CTTTGAGGCG 
AATAGCAATT GTGCCTACGA TGGCGTCGAG GTATTCTCCG GACCCATCCC GGACGAGCGA 
TACCGATGGG GACGCTTCTG CGGTCGCATT AACGAGGATC TGCCACTGAT CAGTATTCCC 
CAAGAGCGGG GCATCATACA CAGCTTCTCG GATGATCGGG ATCCATCGAG GGGATTCCGT 
GCCTTGGTGC GCGTAATGCC AAATTGCGAT GAGAAGATCT CGCTGAACGG ATCGTCGCGT 
TATGTCTATA GCAAATTTAA CAACGCTGGC GGTTATCAAA ACGATTTGGA CTGTCAAATA 
GTATTCCGGG TGAATCCTGA TCAGCAGATA AGCGTGGAGT TCAGTAATTT CCATGTCCAA 
GACACGGACG GATGCCGCAG TGATTATGTG GAGTTGCGCG ATGGTGGCGG AACCTTTGCC 
GACATTATCG GACGTTTTTG TGGCCAGAAT CAACCGCCCA CCTTGAGAAC AACCAGGCAT 
ACGCTCTATA TGCGCTTTGT CACCGATAAT AAGGTGACGG ATACGGGTTT CCAGGTGACC 
ATCAATGCTA TTCCCCGACT ATGCGGCAGT TCAGAGATTA CGTTGAGTGC GGATGGCACC 
AAGGAGGTCA CCATTAATTC TCCGGCAAGG ACGCCAGGTG GCAATTATCC CAATGGAGTG 
TCGTGCTTCT GGAAAATTAA GGGCGATTCC CTGCTGAGGG TGCAGTTCGT CAACTTTGAT 
CTCCACGGAC CGAATCAAAA TGGTAGCTGC GTGGATGACT ATTTGAAAAT CTACAATAGT 
GAGGATGCAC CGCTGTTGGA GCAGGGATTG GGCACCGACC TGGTATTCAA TGGCCAGACC 
TCCAGTAAGA ACGGTTTCGG CTTTGCCACG GAGCATGTGT ACTGCGGCAA TGTGAAGCCG 
GACATCTATT ATGGCCGGTC CAGCGAGGTG TATCTGAAGT TCCGGTCAAA GGGATTGGAG 
CAGCATGGTG GATTTCAGTT ACAGGTCGCT CTGAACTCCA ACCGAGAGCG CCACTACGAT 
GGACTGCAGG GCAGAGTGCA TTTATCACAG TCGGCCGATT GTAACATCAT TATTCGGGCG 
CCACCAAATT ACACCTTAAG TTTGTACTAC ACCGAATTGA TATTCGGCAC CTACGACTGC 
GAAATGGAGA ATTTGGAGGT GTTCGACAGG ACGAATCGAT CGCTGCAGCG CGTCTGCTCC 
TTTGTGGACA TGGGCAAGAG CCTGTTCAGC AATGCGAATG AACTGCGGCT ACAGATGAAG 
ACTGGCTCGT ACTTGACCTC GCTGGATCTC ACCTATTTGG CCAGTCCGGT GGAGAAGGGT 
CCTGGTTGCG GTGGTCAATT CTACAATACC GAGGGCATAT TCTCAAATCC CTTCTATCCG 
AACAATGTGC GCAATAACTC AGAGTGTCAG TGGATTGTCC GCGTGCCGAG CAACAATGTT 
GTGTTCCTTA CATTTGAAGT CTTCAATCTG GGCTCGAAGA CCACCTGTCA CACGGACTAC 
CTGCAAATCC TGGAACAGGA CGCAACCGGT GAGGAGCGCG AAATGAGACG CTTCTGTGGC 
GAGGATAACC CAAAGTACTA CAAGAGCCGG CGCAGCCAGG TGCTCGTCCG CTTTCACAAG 
ACCGTCAACT ACGATGGTAT CGGTTGGGTG ATCCGCTTTG CCGGTGTCTA CTCCGACCAT 
CAGATACCCA GACACCTGTT GGGCGGTTCT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Cubn cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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