ARHGEF2 cDNA ORF clone, Canis lupus familiaris(dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	ARHGEF2
Entrez Gene ID	403498
Full Name	Rho/Rac guanine nucleotide exchange factor 2
Synonyms	GEF-H1,GEF-H1/LFC
General protein information	Preferred Names Rho/Rac guanine nucleotide exchange factor 2 Names rho guanine nucleotide exchange factor 2 Guanine nucleotide exchange factor H1 tight junction-associated guanine nucleotide exchange factor
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001002995.1	NP_001002995.1	rho guanine nucleotide exchange factor 2 isoform A
NM_001006123.1	NP_001006123.1	rho guanine nucleotide exchange factor 2 isoform B

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	ARHGEF2	XP_003308546.1
	ARHGEF2	XP_002801891.1
Bos taurus (cattle)	ARHGEF2	NP_001092351.2
Rattus norvegicus (Norway rat)	Arhgef2	NP_001012079.1
Danio rerio (zebrafish)	arhgef1a	NP_001075100.1
Homo sapiens (human)	ARHGEF2	NP_001155855.1
Canis lupus familiaris (dog)	ARHGEF2	NP_001002995.1
Danio rerio (zebrafish)	arhgef2	XP_689770.5
Xenopus tropicalis (tropical clawed frog)	arhgef2	NP_001001456.1
Mus musculus (house mouse)	Arhgef2	NP_032513.3

Gene Ontology
Bibliography

Related articles in PubMed

Stress- and Rho-activated ZO-1-associated nucleic acid binding protein binding to p21 mRNA mediates stabilization, translation, and cell survival.
Nie M, Balda MS, Matter K
Proceedings of the National Academy of Sciences of the United States of America109(27)10897-902(2012 Jul)

The epidermal growth factor receptor mediates tumor necrosis factor-alpha-induced activation of the ERK/GEF-H1/RhoA pathway in tubular epithelium.
Kakiashvili E, Dan Q, Vandermeer M, Zhang Y, Waheed F, Pham M, Sz?szi K
The Journal of biological chemistry286(11)9268-79(2011 Mar)

The Y-box factor ZONAB/DbpA associates with GEF-H1/Lfc and mediates Rho-stimulated transcription.
Nie M, Aijaz S, Leefa Chong San IV, Balda MS, Matter K
EMBO reports10(10)1125-31(2009 Oct)

GEF-H1 mediates tumor necrosis factor-alpha-induced Rho activation and myosin phosphorylation: role in the regulation of tubular paracellular permeability.
Kakiashvili E, Speight P, Waheed F, Seth R, Lodyga M, Tanimura S, Kohno M, Rotstein OD, Kapus A, Sz?szi K
The Journal of biological chemistry284(17)11454-66(2009 Apr)

GEF-H1 mediated control of NOD1 dependent NF-kappaB activation by Shigella effectors.
Fukazawa A, Alonso C, Kurachi K, Gupta S, Lesser CF, McCormick BA, Reinecker HC
PLoS pathogens4(11)e1000228(2008 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

the TNF-alpha-induced activation of the ERK/GEF-H1/RhoA pathway in tubular cells is mediated through Src- and TACE-dependent EGFR activation.
Title: The epidermal growth factor receptor mediates tumor necrosis factor-alpha-induced activation of the ERK/GEF-H1/RhoA pathway in tubular epithelium.

data thus indicate that GEF-H1 and ZONAB form a signalling module that mediates Rho-regulated cyclin D1 promoter activation and expression.
Title: The Y-box factor ZONAB/DbpA associates with GEF-H1/Lfc and mediates Rho-stimulated transcription.

the ERK/GEF-H1/Rho/Rho kinase/phospho-MLC pathway is the mechanism mediating TNF-alpha-induced elevation of tubular epithelial permeability, which in turn might contribute to kidney injury
Title: GEF-H1 mediates tumor necrosis factor-alpha-induced Rho activation and myosin phosphorylation: role in the regulation of tubular paracellular permeability.

GEF-H1 is a critical component of cellular defenses forming an intracellular sensing system with NOD1 for the detection of microbial effectors during cell invasion by pathogens.
Title: GEF-H1 mediated control of NOD1 dependent NF-kappaB activation by Shigella effectors.

Paracingulin regulates the activity of Rac1 and RhoA GTPases by recruiting Tiam1 and GEF-H1 to epithelial junctions
Title: Paracingulin regulates the activity of Rac1 and RhoA GTPases by recruiting Tiam1 and GEF-H1 to epithelial junctions.

The following ARHGEF2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ARHGEF2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCe34255	NM_001002995.1 Latest version!	Canis lupus familiaris Rho/Rac guanine nucleotide exchange factor 2 (ARHGEF2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
OCe34280	NM_001006123.1 Latest version!	Canis lupus familiaris Rho/Rac guanine nucleotide exchange factor 2 (ARHGEF2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ARHGEF2 ( NM_001002995.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> NP_001002995.1 Canis lupus familiaris Rho/Rac guanine nucleotide exchange factor 2 (ARHGEF2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OCe34255
Clone ID Related Accession (Same CDS sequence)	NM_001002995.1
Accession Version	NM_001002995.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2961bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-15
Organism	Canis lupus familiaris(dog)
Product	rho guanine nucleotide exchange factor 2 isoform A
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF494096.1. ##Evidence-Data-START## Transcript exon combination :: AF494096.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA103936001, SAMEA103936002 [ECO:0000350] ##Evidence-Data-END##

ATGTCTCGGA TCGAGTCGCT CACGCGCGCG CGGACCGAGC GGAGCCGGGA GCTGGCGAGC 
AAGAATCGGG AAAAGGAGAA GATGAAGGAA GCCAAGGACG CCCGCTACAC CAACGGGCAC 
CTCTTTACCA CCATCTCCGT CTCGGGCATG ACCATGTGCT ATGCCTGTAA CAAGAGCATC 
ACAGCCAAGG AAGCCCTTAT CTGCCCAACC TGCAATGTGA CTATCCACAA CCGCTGTAAA 
GACACGCTCG CCAACTGTAC CAAGGTCAAG CAGAAGCAAC AGAAGGCTGC TCTGCTGAAG 
AACAGCACTG CTTTGCAGTC TGTTTCCCTT CGCAGTAAGA CCACCCCCCG GGAGCGGCCA 
AGCTCTGCCA TCTACCCCTC TGACAGCTTC CGGCAGTCCC TGCTCGGCTC CCGCCGTGGC 
CGCTCCTCCT TGTCATTAGC CAAGAGTGTC TCCACCACCA ACATTGCCGG CCACTTCAGT 
GACGAGTCAC CCCTGGGGCT GCGCCGAATC CTGTCCCAGT CCACAGACTC CCTCAACATG 
CGGAACCGAG CCCTATCCGT GGAGTCCCTC ATTGACGAAG GTGCAGAAGT GATCTACAGT 
GAGCTCATGA GTGACTTTGA GACAGATGAG AGGGACTTTG CAGCCGACTC ATGGAGCTTG 
GCAGTAGACA GCAGCTTCCT GCAGCAGCAG AAGAAAGAGG TGATGAAGCA GCAGGATGTC 
ATCTATGAGC TCATCCAGAC GGAGCTGCAC CATGTGCGGA CGCTGAAGAT CATGACCCGC 
CTCTTCCGCA CGGGGATGCT AGAGGAGCTG CAGCTGGAGC CCGGAGTGGT CCAGGGTCTG 
TTCCCCTGCG TGGATGAGCT CAGTGACATC CACACACGCT TCCTCAGCCA GCTCCTAGAA 
CGCCGACGCC AAGCCCTGTG CCCTGGAAGC ACCCGGAACT TCGTCATCCA TCGCCTGGCA 
GACCTGCTCA TCAGCCAGTT CTCAGGTCCC AGTGCGGAGC GGATGCGTAA GGCCTACTCA 
GAGTTCTGCA GCCGCCACAC TAAGGCCTTA AAGCTTTACA AGGAGCTGTA TGCCCGGGAC 
AAAAGGTTCC AGCAGTTCAT CCGGAAGGTG ACCCGATCGG CTGTGTTGAA GCGTCATGGG 
GTGCAGGAGT GCATCCTGCT GGTGACCCAG CGCATCACCA AGTACCCGGT ACTCATCAAC 
CGGATCCTAC AGCATTCCCA TGGGACCGAC GAGGAGCGCC AGGACCTGAC CACAGCACTG 
GGGCTGGTGA AGGAGCTCCT GTCCAATGTG GACCAGGACG TGCATGAGCT GGAGAAAGGA 
GCTCGCCTGC AGGAGATCTA CCACCGGATG GACCCTCGGG CCCAGGCCCC AGTGCCCAGC 
AAGGGCCCAT TTGGCAGAGA GGAGCTTCTG CGGCGCAAGC TCATCCATGA TGGTTGCCTG 
CTCTGGAAGA CAGCGACAGG TCGCTTCAAA GATGTGCTCA TGCTCCTGAT GACAGACGTG 
CTGGTATTTC TCCAGGAGAA GGACCAGAAG TACATCTTTC CTGCCCTGGA CAAGCCCTCA 
GTGGTATCAC TGCAGAATCT GATTGTACGG GACATCGCCA ACCAGGAGAA AGGGATGTTT 
CTGATCAGTG CGGCGCCCCC TGAGATGTAT GAGGTCCACA CCGCATCTCG GGATGACCGG 
AGCACCTGGG TCCGGGTCAT TCAGCAGAGT GTGCGCGTAT GCCCATCCAG AGAGGACTTC 
CCCCTGATTG AGACAGAGGA CGAGGCTTAC CTGCGCCGAA TCAAGATGGA GCTGCAACAG 
AAAGACAAGG CGTTGGTGGA GTTGCTGCGA GAGAAGGTGG GACTGTTTGC CGAGATGACC 
CATTTCCAAG TGGAGGAGGA CAGTGGCGGG GTGGCCCTGC CCGCCCTACC CAGGGGTCTT 
TTCCGCTCCG AGTCCTTGGA GTGCCCTCGT GGCGAGCGGC TGCTGCAGGA TGCCATCCGT 
GAGGTGGAGG GCCTCAAAGA CCTCCTGGTG GGGCCTGGAG TAGAGCTGCT CCTGACACCG 
CGGGATCCGG CCTTGCTTGT GGACCCAGAC AGTGGCGGTA GCACGAGTCC TGGGGTCACA 
GCCAATGGTG AGGCCAGAAA CTTCAATGGC TCCATTGAGC TCTGCAGAAC GGACTCTGAC 
TCCAGTCAGA AGGACCGCAA TGGGAATCAG TTGAGAGCCC CCCAGGAGGA AGCATTACAG 
CGATTGGTCA ATCTCTACGG ACTCCTCCAT GGCCTCCAGG CGGCCGTGGC CCAGCAGGAC 
ACCCTGATGG AAGCCCGGTT CCCTGAGGGC CCTGAGCGGC GGGAGAAGCT GGCCAGAGCC 
AACTCCAGGG ATGGAGAGGC TGGCCGAGTG GGGCCCGCCC CAGTGGCACC TGACAAGCAG 
GCCACCGAGC TGGCGCTCCT GCAGCGGCAA CATGCTCTGC TGCAGGAGGA GCTGCGTCGC 
TGCCGGCGGC TGGGCGAGGA GCGCGCCACG GAGGCGGGCA ACCTGGAGGC CCGGCTCCGC 
GAGAGTGAAC AGGCCCGGGC CCTGCTGGAG AGGGAGGCGG AGGAGGCCCG CAGGCAGCTG 
GCCATCTTGG GGCAGAGTGA GCCGCCCCCG GCGGAGGCCC CCTGGGCCCG CAGGCCCTTG 
GACCCTCGGC GGCGCAGCCT CCCTGCAGGC GATGCTCTGT ACCTGAGCTT CACGCCCCCA 
CAGCCCAGCC GAGGCCACGA CCGTCTGGAT TTGTCTGTGA CCATTCGCTC TGTCCATCGA 
CCCTTTGAGG ACCGAGAGAG GCAGGAGCTG GGCAGCCCCG AGGAGCGGCT GCAGGACAGC 
AGTGACCCTG ATACCGGCAG CGAGGAGGAA GGGGGCGGCC GCCTGTCCCC ACCCCACAGT 
CCTCGAGATT TCACCCGAAT GCAAGACATC CCTGAAGAGA CAGAGAGTCG TGACGGGGAG 
CCTATGGTTT CAGAGAGCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001002995.1
CDS	1..2961
Translation	MSRIESLTRARTERSRELASKNREKEKMKEAKDARYTNGHLFTTISVSGMTMCYACNKSITAKEALICPTCNVTIHNRCKDTLANCTKVKQKQQKAALLKNSTALQSVSLRSKTTPRERPSSAIYPSDSFRQSLLGSRRGRSSLSLAKSVSTTNIAGHFSDESPLGLRRILSQSTDSLNMRNRALSVESLIDEGAEVIYSELMSDFETDERDFAADSWSLAVDSSFLQQQKKEVMKQQDVIYELIQTELHHVRTLKIMTRLFRTGMLEELQLEPGVVQGLFPCVDELSDIHTRFLSQLLERRRQALCPGSTRNFVIHRLADLLISQFSGPSAERMRKAYSEFCSRHTKALKLYKELYARDKRFQQFIRKVTRSAVLKRHGVQECILLVTQRITKYPVLINRILQHSHGTDEERQDLTTALGLVKELLSNVDQDVHELEKGARLQEIYHRMDPRAQAPVPSKGPFGREELLRRKLIHDGCLLWKTATGRFKDVLMLLMTDVLVFLQEKDQKYIFPALDKPSVVSLQNLIVRDIANQEKGMFLISAAPPEMYEVHTASRDDRSTWVRVIQQSVRVCPSREDFPLIETEDEAYLRRIKMELQQKDKALVELLREKVGLFAEMTHFQVEEDSGGVALPALPRGLFRSESLECPRGERLLQDAIREVEGLKDLLVGPGVELLLTPRDPALLVDPDSGGSTSPGVTANGEARNFNGSIELCRTDSDSSQKDRNGNQLRAPQEEALQRLVNLYGLLHGLQAAVAQQDTLMEARFPEGPERREKLARANSRDGEAGRVGPAPVAPDKQATELALLQRQHALLQEELRRCRRLGEERATEAGNLEARLRESEQARALLEREAEEARRQLAILGQSEPPPAEAPWARRPLDPRRRSLPAGDALYLSFTPPQPSRGHDRLDLSVTIRSVHRPFEDRERQELGSPEERLQDSSDPDTGSEEEGGGRLSPPHSPRDFTRMQDIPEETESRDGEPMVSES

Target ORF information:

RefSeq Version	NM_001002995.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris Rho/Rac guanine nucleotide exchange factor 2 (ARHGEF2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001002995.1

ORF Insert Sequence:

ATGTCTCGGA TCGAGTCGCT CACGCGCGCG CGGACCGAGC GGAGCCGGGA GCTGGCGAGC 
AAGAATCGGG AAAAGGAGAA GATGAAGGAA GCCAAGGACG CCCGCTACAC CAACGGGCAC 
CTCTTTACCA CCATCTCCGT CTCGGGCATG ACCATGTGCT ATGCCTGTAA CAAGAGCATC 
ACAGCCAAGG AAGCCCTTAT CTGCCCAACC TGCAATGTGA CTATCCACAA CCGCTGTAAA 
GACACGCTCG CCAACTGTAC CAAGGTCAAG CAGAAGCAAC AGAAGGCTGC TCTGCTGAAG 
AACAGCACTG CTTTGCAGTC TGTTTCCCTT CGCAGTAAGA CCACCCCCCG GGAGCGGCCA 
AGCTCTGCCA TCTACCCCTC TGACAGCTTC CGGCAGTCCC TGCTCGGCTC CCGCCGTGGC 
CGCTCCTCCT TGTCATTAGC CAAGAGTGTC TCCACCACCA ACATTGCCGG CCACTTCAGT 
GACGAGTCAC CCCTGGGGCT GCGCCGAATC CTGTCCCAGT CCACAGACTC CCTCAACATG 
CGGAACCGAG CCCTATCCGT GGAGTCCCTC ATTGACGAAG GTGCAGAAGT GATCTACAGT 
GAGCTCATGA GTGACTTTGA GACAGATGAG AGGGACTTTG CAGCCGACTC ATGGAGCTTG 
GCAGTAGACA GCAGCTTCCT GCAGCAGCAG AAGAAAGAGG TGATGAAGCA GCAGGATGTC 
ATCTATGAGC TCATCCAGAC GGAGCTGCAC CATGTGCGGA CGCTGAAGAT CATGACCCGC 
CTCTTCCGCA CGGGGATGCT AGAGGAGCTG CAGCTGGAGC CCGGAGTGGT CCAGGGTCTG 
TTCCCCTGCG TGGATGAGCT CAGTGACATC CACACACGCT TCCTCAGCCA GCTCCTAGAA 
CGCCGACGCC AAGCCCTGTG CCCTGGAAGC ACCCGGAACT TCGTCATCCA TCGCCTGGCA 
GACCTGCTCA TCAGCCAGTT CTCAGGTCCC AGTGCGGAGC GGATGCGTAA GGCCTACTCA 
GAGTTCTGCA GCCGCCACAC TAAGGCCTTA AAGCTTTACA AGGAGCTGTA TGCCCGGGAC 
AAAAGGTTCC AGCAGTTCAT CCGGAAGGTG ACCCGATCGG CTGTGTTGAA GCGTCATGGG 
GTGCAGGAGT GCATCCTGCT GGTGACCCAG CGCATCACCA AGTACCCGGT ACTCATCAAC 
CGGATCCTAC AGCATTCCCA TGGGACCGAC GAGGAGCGCC AGGACCTGAC CACAGCACTG 
GGGCTGGTGA AGGAGCTCCT GTCCAATGTG GACCAGGACG TGCATGAGCT GGAGAAAGGA 
GCTCGCCTGC AGGAGATCTA CCACCGGATG GACCCTCGGG CCCAGGCCCC AGTGCCCAGC 
AAGGGCCCAT TTGGCAGAGA GGAGCTTCTG CGGCGCAAGC TCATCCATGA TGGTTGCCTG 
CTCTGGAAGA CAGCGACAGG TCGCTTCAAA GATGTGCTCA TGCTCCTGAT GACAGACGTG 
CTGGTATTTC TCCAGGAGAA GGACCAGAAG TACATCTTTC CTGCCCTGGA CAAGCCCTCA 
GTGGTATCAC TGCAGAATCT GATTGTACGG GACATCGCCA ACCAGGAGAA AGGGATGTTT 
CTGATCAGTG CGGCGCCCCC TGAGATGTAT GAGGTCCACA CCGCATCTCG GGATGACCGG 
AGCACCTGGG TCCGGGTCAT TCAGCAGAGT GTGCGCGTAT GCCCATCCAG AGAGGACTTC 
CCCCTGATTG AGACAGAGGA CGAGGCTTAC CTGCGCCGAA TCAAGATGGA GCTGCAACAG 
AAAGACAAGG CGTTGGTGGA GTTGCTGCGA GAGAAGGTGG GACTGTTTGC CGAGATGACC 
CATTTCCAAG TGGAGGAGGA CAGTGGCGGG GTGGCCCTGC CCGCCCTACC CAGGGGTCTT 
TTCCGCTCCG AGTCCTTGGA GTGCCCTCGT GGCGAGCGGC TGCTGCAGGA TGCCATCCGT 
GAGGTGGAGG GCCTCAAAGA CCTCCTGGTG GGGCCTGGAG TAGAGCTGCT CCTGACACCG 
CGGGATCCGG CCTTGCTTGT GGACCCAGAC AGTGGCGGTA GCACGAGTCC TGGGGTCACA 
GCCAATGGTG AGGCCAGAAA CTTCAATGGC TCCATTGAGC TCTGCAGAAC GGACTCTGAC 
TCCAGTCAGA AGGACCGCAA TGGGAATCAG TTGAGAGCCC CCCAGGAGGA AGCATTACAG 
CGATTGGTCA ATCTCTACGG ACTCCTCCAT GGCCTCCAGG CGGCCGTGGC CCAGCAGGAC 
ACCCTGATGG AAGCCCGGTT CCCTGAGGGC CCTGAGCGGC GGGAGAAGCT GGCCAGAGCC 
AACTCCAGGG ATGGAGAGGC TGGCCGAGTG GGGCCCGCCC CAGTGGCACC TGACAAGCAG 
GCCACCGAGC TGGCGCTCCT GCAGCGGCAA CATGCTCTGC TGCAGGAGGA GCTGCGTCGC 
TGCCGGCGGC TGGGCGAGGA GCGCGCCACG GAGGCGGGCA ACCTGGAGGC CCGGCTCCGC 
GAGAGTGAAC AGGCCCGGGC CCTGCTGGAG AGGGAGGCGG AGGAGGCCCG CAGGCAGCTG 
GCCATCTTGG GGCAGAGTGA GCCGCCCCCG GCGGAGGCCC CCTGGGCCCG CAGGCCCTTG 
GACCCTCGGC GGCGCAGCCT CCCTGCAGGC GATGCTCTGT ACCTGAGCTT CACGCCCCCA 
CAGCCCAGCC GAGGCCACGA CCGTCTGGAT TTGTCTGTGA CCATTCGCTC TGTCCATCGA 
CCCTTTGAGG ACCGAGAGAG GCAGGAGCTG GGCAGCCCCG AGGAGCGGCT GCAGGACAGC 
AGTGACCCTG ATACCGGCAG CGAGGAGGAA GGGGGCGGCC GCCTGTCCCC ACCCCACAGT 
CCTCGAGATT TCACCCGAAT GCAAGACATC CCTGAAGAGA CAGAGAGTCG TGACGGGGAG 
CCTATGGTTT CAGAGAGCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGEF2 ( NM_001006123.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> NP_001006123.1 Canis lupus familiaris Rho/Rac guanine nucleotide exchange factor 2 (ARHGEF2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OCe34280
Clone ID Related Accession (Same CDS sequence)	NM_001006123.1
Accession Version	NM_001006123.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2895bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-18
Organism	Canis lupus familiaris(dog)
Product	rho guanine nucleotide exchange factor 2 isoform B
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF494097.1. ##Evidence-Data-START## Transcript exon combination :: AF494097.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA103936001, SAMEA103936002 [ECO:0000348] ##Evidence-Data-END##

ATGTCTCGGA TCGAGTCGCT CACGCGCGCG CGGACCGAGC GGAGCCGGGA GCTGGCGAGC 
AAGAATCGGG AAAAGGAGAA GATGAAGGAA GCCAAGGACG CCCGCTACAC CAACGGGCAC 
CTCTTTACCA CCATCTCCGT CTCGGGCATG ACCATGTGCT ATGCCTGTAA CAAGAGCATC 
ACAGCCAAGG AAGCCCTTAT CTGCCCAACC TGCAATGTGA CTATCCACAA CCGCTGTAAA 
GACACGCTCG CCAACTGTAC CAAGGTCAAG CAGAAGCAAC AGAAGGCTGC TCTGCTGAAG 
AACAGCACTG CTTTGCAGTC TGTTTCCCTT CGCAGTAAGA CCACCCCCCG GGAGCGGCCA 
AGCTCTGCCA TCTACCCCTC TGACAGCTTC CGGCAGTCCC TGCTCGGCTC CCGCCGTGGC 
CGCTCCTCCT TGTCATTAGC CAAGAGTGTC TCCACCACCA ACATTGCCGG CCACTTCAGT 
GACGAGTCAC CCCTGGGGCT GCGCCGAATC CTGTCCCAGT CCACAGACTC CCTCAACATG 
CGGAACCGAG CCCTATCCGT GGAGTCCCTC ATTGACGAAG GTGCAGAAGT GATCTACAGT 
GAGCTCATGA GTGACTTTGA GACAGATGAG AGGGACTTTG CAGCCGACTC ATGGAGCTTG 
GCAGTAGACA GCAGCTTCCT GCAGCAGCAG AAGAAAGAGG TGATGAAGCA GCAGGATGTC 
ATCTATGAGC TCATCCAGAC GGAGCTGCAC CATGTGCGGA CGCTGAAGAT CATGACCCGC 
CTCTTCCGCA CGGGGATGCT AGAGGAGCTG CAGCTGGAGC CCGGAGTGGT CCAGGGTCTG 
TTCCCCTGCG TGGATGAGCT CAGTGACATC CACACACGCT TCCTCAGCCA GCTCCTAGAA 
CGCCGACGCC AAGCCCTGTG CCCTGGAAGC ACCCGGAACT TCGTCATCCA TCGCCTGGCA 
GACCTGCTCA TCAGCCAGTT CTCAGGTCCC AGTGCGGAGC GGATGCGTAA GGCCTACTCA 
GAGTTCTGCA GCCGCCACAC TAAGGCCTTA AAGCTTTACA AGGAGCTGTA TGCCCGGGAC 
AAAAGGTTCC AGCAGTTCAT CCGGAAGGTG ACCCGATCGG CTGTGTTGAA GCGTCATGGG 
GTGCAGGAGT GCATCCTGCT GGTGACCCAG CGCATCACCA AGTACCCGGT ACTCATCAAC 
CGGATCCTAC AGCATTCCCA TGGGACCGAC GAGGAGCGCC AGGACCTGAC CACAGCACTG 
GGGCTGGTGA AGGAGCTCCT GTCCAATGTG GACCAGGACG TGCATGAGCT GGAGAAAGGA 
GCTCGCCTGC AGGAGATCTA CCACCGGATG GACCCTCGGG CCCAGGCCCC AGTGCCCAGC 
AAGGGCCCAT TTGGCAGAGA GGAGCTTCTG CGGCGCAAGC TCATCCATGA TGGTTGCCTG 
CTCTGGAAGA CAGCGACAGG TCGCTTCAAA GATGTGCTCA TGCTCCTGAT GACAGACGTG 
CTGGTATTTC TCCAGGAGAA GGACCAGAAG TACATCTTTC CTGCCCTGGA CAAGCCCTCA 
GTGGTATCAC TGCAGAATCT GATTGTACGG GACATCGCCA ACCAGGAGAA AGGGATGTTT 
CTGATCAGTG CGGCGCCCCC TGAGATGTAT GAGGTCCACA CCGCATCTCG GGATGACCGG 
AGCACCTGGG TCCGGGTCAT TCAGCAGAGT GTGCGCGTAT GCCCATCCAG AGAGGACTTC 
CCCCTGATTG AGACAGAGGA CGAGGCTTAC CTGCGCCGAA TCAAGATGGA GCTGCAACAG 
AAAGACAAGG CGTTGGTGGA GTTGCTGCGA GAGAAGGTGG GACTGTTTGC CGAGATGACC 
CATTTCCAAG TGGAGGAGGA CAGTGGCGGG GTGGCCCTGC CCGCCCTACC CAGGGGTCTT 
TTCCGCTCCG AGTCCTTGGA GTGCCCTCGT GGCGAGCGGC TGCTGCAGGA TGCCATCCGT 
GAGGTGGAGG GCCTCAAAGA CCTCCTGGTG GGGCCTGGAG TAGAGCTGCT CCTGACACCG 
CGGGATCCGG CCTTGCTTGT GGACCCAGAC AGTGGCGGTA GCACGAGTCC TGGGGTCACA 
GCCAATGGTG AGGCCAGAAA CTTCAATGGC TCCATTGAGC TCTGCAGAAC GGACTCTGAC 
TCCAGTCAGA AGGACCGCAA TGGGAATCAG TTGAGAGCCC CCCAGGAGGA AGCATTACAG 
CGATTGGTCA ATCTCTACGG ACTCCTCCAT GGCCTCCAGG CGGCCGTGGC CCAGCAGGAC 
ACCCTGATGG AAGCCCGGTT CCCTGAGGGC CCTGAGCGGC GGGAGAAGCT GGCCAGAGCC 
AACTCCAGGG ATGGAGAGGC TGGCCGAGTG GGGCCCGCCC CAGTGGCACC TGACAAGCAG 
GCCACCGAGC TGGCGCTCCT GCAGCGGCAA CATGCTCTGC TGCAGGAGGA GCTGCGTCGC 
TGCCGGCGGC TGGGCGAGGA GCGCGCCACG GAGGCGGGCA ACCTGGAGGC CCGGCTCCGC 
GAGAGTGAAC AGGCCCGGGC CCTGCTGGAG AGGGAGGCGG AGGAGGCCCG CAGGCAGCTG 
GCCATCTTGG GGCAGAGTGA GCCGCCCCCG GCGGAGGCCC CCTGGGCCCG CAGGCCCTTG 
GACCCTCGGC GGCGCAGCCT CCCTGCAGGC GATGCTCTGT ACCTGAGCTT CACGCCCCCA 
CAGGACCGAG AGAGGCAGGA GCTGGGCAGC CCCGAGGAGC GGCTGCAGGA CAGCAGTGAC 
CCTGATACCG GCAGCGAGGA GGAAGGGGGC GGCCGCCTGT CCCCACCCCA CAGTCCTCGA 
GATTTCACCC GAATGCAAGA CATCCCTGAA GAGACAGAGA GTCGTGAAGG GGAGCCTATG 
GTTTCAGAGA GCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001006123.1
CDS	1..2895
Translation	MSRIESLTRARTERSRELASKNREKEKMKEAKDARYTNGHLFTTISVSGMTMCYACNKSITAKEALICPTCNVTIHNRCKDTLANCTKVKQKQQKAALLKNSTALQSVSLRSKTTPRERPSSAIYPSDSFRQSLLGSRRGRSSLSLAKSVSTTNIAGHFSDESPLGLRRILSQSTDSLNMRNRALSVESLIDEGAEVIYSELMSDFETDERDFAADSWSLAVDSSFLQQQKKEVMKQQDVIYELIQTELHHVRTLKIMTRLFRTGMLEELQLEPGVVQGLFPCVDELSDIHTRFLSQLLERRRQALCPGSTRNFVIHRLADLLISQFSGPSAERMRKAYSEFCSRHTKALKLYKELYARDKRFQQFIRKVTRSAVLKRHGVQECILLVTQRITKYPVLINRILQHSHGTDEERQDLTTALGLVKELLSNVDQDVHELEKGARLQEIYHRMDPRAQAPVPSKGPFGREELLRRKLIHDGCLLWKTATGRFKDVLMLLMTDVLVFLQEKDQKYIFPALDKPSVVSLQNLIVRDIANQEKGMFLISAAPPEMYEVHTASRDDRSTWVRVIQQSVRVCPSREDFPLIETEDEAYLRRIKMELQQKDKALVELLREKVGLFAEMTHFQVEEDSGGVALPALPRGLFRSESLECPRGERLLQDAIREVEGLKDLLVGPGVELLLTPRDPALLVDPDSGGSTSPGVTANGEARNFNGSIELCRTDSDSSQKDRNGNQLRAPQEEALQRLVNLYGLLHGLQAAVAQQDTLMEARFPEGPERREKLARANSRDGEAGRVGPAPVAPDKQATELALLQRQHALLQEELRRCRRLGEERATEAGNLEARLRESEQARALLEREAEEARRQLAILGQSEPPPAEAPWARRPLDPRRRSLPAGDALYLSFTPPQDRERQELGSPEERLQDSSDPDTGSEEEGGGRLSPPHSPRDFTRMQDIPEETESREGEPMVSES

Target ORF information:

RefSeq Version	NM_001006123.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris Rho/Rac guanine nucleotide exchange factor 2 (ARHGEF2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001006123.1

ORF Insert Sequence:

ATGTCTCGGA TCGAGTCGCT CACGCGCGCG CGGACCGAGC GGAGCCGGGA GCTGGCGAGC 
AAGAATCGGG AAAAGGAGAA GATGAAGGAA GCCAAGGACG CCCGCTACAC CAACGGGCAC 
CTCTTTACCA CCATCTCCGT CTCGGGCATG ACCATGTGCT ATGCCTGTAA CAAGAGCATC 
ACAGCCAAGG AAGCCCTTAT CTGCCCAACC TGCAATGTGA CTATCCACAA CCGCTGTAAA 
GACACGCTCG CCAACTGTAC CAAGGTCAAG CAGAAGCAAC AGAAGGCTGC TCTGCTGAAG 
AACAGCACTG CTTTGCAGTC TGTTTCCCTT CGCAGTAAGA CCACCCCCCG GGAGCGGCCA 
AGCTCTGCCA TCTACCCCTC TGACAGCTTC CGGCAGTCCC TGCTCGGCTC CCGCCGTGGC 
CGCTCCTCCT TGTCATTAGC CAAGAGTGTC TCCACCACCA ACATTGCCGG CCACTTCAGT 
GACGAGTCAC CCCTGGGGCT GCGCCGAATC CTGTCCCAGT CCACAGACTC CCTCAACATG 
CGGAACCGAG CCCTATCCGT GGAGTCCCTC ATTGACGAAG GTGCAGAAGT GATCTACAGT 
GAGCTCATGA GTGACTTTGA GACAGATGAG AGGGACTTTG CAGCCGACTC ATGGAGCTTG 
GCAGTAGACA GCAGCTTCCT GCAGCAGCAG AAGAAAGAGG TGATGAAGCA GCAGGATGTC 
ATCTATGAGC TCATCCAGAC GGAGCTGCAC CATGTGCGGA CGCTGAAGAT CATGACCCGC 
CTCTTCCGCA CGGGGATGCT AGAGGAGCTG CAGCTGGAGC CCGGAGTGGT CCAGGGTCTG 
TTCCCCTGCG TGGATGAGCT CAGTGACATC CACACACGCT TCCTCAGCCA GCTCCTAGAA 
CGCCGACGCC AAGCCCTGTG CCCTGGAAGC ACCCGGAACT TCGTCATCCA TCGCCTGGCA 
GACCTGCTCA TCAGCCAGTT CTCAGGTCCC AGTGCGGAGC GGATGCGTAA GGCCTACTCA 
GAGTTCTGCA GCCGCCACAC TAAGGCCTTA AAGCTTTACA AGGAGCTGTA TGCCCGGGAC 
AAAAGGTTCC AGCAGTTCAT CCGGAAGGTG ACCCGATCGG CTGTGTTGAA GCGTCATGGG 
GTGCAGGAGT GCATCCTGCT GGTGACCCAG CGCATCACCA AGTACCCGGT ACTCATCAAC 
CGGATCCTAC AGCATTCCCA TGGGACCGAC GAGGAGCGCC AGGACCTGAC CACAGCACTG 
GGGCTGGTGA AGGAGCTCCT GTCCAATGTG GACCAGGACG TGCATGAGCT GGAGAAAGGA 
GCTCGCCTGC AGGAGATCTA CCACCGGATG GACCCTCGGG CCCAGGCCCC AGTGCCCAGC 
AAGGGCCCAT TTGGCAGAGA GGAGCTTCTG CGGCGCAAGC TCATCCATGA TGGTTGCCTG 
CTCTGGAAGA CAGCGACAGG TCGCTTCAAA GATGTGCTCA TGCTCCTGAT GACAGACGTG 
CTGGTATTTC TCCAGGAGAA GGACCAGAAG TACATCTTTC CTGCCCTGGA CAAGCCCTCA 
GTGGTATCAC TGCAGAATCT GATTGTACGG GACATCGCCA ACCAGGAGAA AGGGATGTTT 
CTGATCAGTG CGGCGCCCCC TGAGATGTAT GAGGTCCACA CCGCATCTCG GGATGACCGG 
AGCACCTGGG TCCGGGTCAT TCAGCAGAGT GTGCGCGTAT GCCCATCCAG AGAGGACTTC 
CCCCTGATTG AGACAGAGGA CGAGGCTTAC CTGCGCCGAA TCAAGATGGA GCTGCAACAG 
AAAGACAAGG CGTTGGTGGA GTTGCTGCGA GAGAAGGTGG GACTGTTTGC CGAGATGACC 
CATTTCCAAG TGGAGGAGGA CAGTGGCGGG GTGGCCCTGC CCGCCCTACC CAGGGGTCTT 
TTCCGCTCCG AGTCCTTGGA GTGCCCTCGT GGCGAGCGGC TGCTGCAGGA TGCCATCCGT 
GAGGTGGAGG GCCTCAAAGA CCTCCTGGTG GGGCCTGGAG TAGAGCTGCT CCTGACACCG 
CGGGATCCGG CCTTGCTTGT GGACCCAGAC AGTGGCGGTA GCACGAGTCC TGGGGTCACA 
GCCAATGGTG AGGCCAGAAA CTTCAATGGC TCCATTGAGC TCTGCAGAAC GGACTCTGAC 
TCCAGTCAGA AGGACCGCAA TGGGAATCAG TTGAGAGCCC CCCAGGAGGA AGCATTACAG 
CGATTGGTCA ATCTCTACGG ACTCCTCCAT GGCCTCCAGG CGGCCGTGGC CCAGCAGGAC 
ACCCTGATGG AAGCCCGGTT CCCTGAGGGC CCTGAGCGGC GGGAGAAGCT GGCCAGAGCC 
AACTCCAGGG ATGGAGAGGC TGGCCGAGTG GGGCCCGCCC CAGTGGCACC TGACAAGCAG 
GCCACCGAGC TGGCGCTCCT GCAGCGGCAA CATGCTCTGC TGCAGGAGGA GCTGCGTCGC 
TGCCGGCGGC TGGGCGAGGA GCGCGCCACG GAGGCGGGCA ACCTGGAGGC CCGGCTCCGC 
GAGAGTGAAC AGGCCCGGGC CCTGCTGGAG AGGGAGGCGG AGGAGGCCCG CAGGCAGCTG 
GCCATCTTGG GGCAGAGTGA GCCGCCCCCG GCGGAGGCCC CCTGGGCCCG CAGGCCCTTG 
GACCCTCGGC GGCGCAGCCT CCCTGCAGGC GATGCTCTGT ACCTGAGCTT CACGCCCCCA 
CAGGACCGAG AGAGGCAGGA GCTGGGCAGC CCCGAGGAGC GGCTGCAGGA CAGCAGTGAC 
CCTGATACCG GCAGCGAGGA GGAAGGGGGC GGCCGCCTGT CCCCACCCCA CAGTCCTCGA 
GATTTCACCC GAATGCAAGA CATCCCTGAA GAGACAGAGA GTCGTGAAGG GGAGCCTATG 
GTTTCAGAGA GCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Stress- and Rho-activated ZO-1-associated nucleic acid binding protein binding to p21 mRNA mediates stabilization, translation, and cell survival.
Proceedings of the National Academy of Sciences of the United States of America109(27)10897-902(2012 Jul)
Nie M,Balda MS,Matter K

The epidermal growth factor receptor mediates tumor necrosis factor-alpha-induced activation of the ERK/GEF-H1/RhoA pathway in tubular epithelium.
The Journal of biological chemistry286(11)9268-79(2011 Mar)
Kakiashvili E,Dan Q,Vandermeer M,Zhang Y,Waheed F,Pham M,Sz?szi K

The Y-box factor ZONAB/DbpA associates with GEF-H1/Lfc and mediates Rho-stimulated transcription.
EMBO reports10(10)1125-31(2009 Oct)
Nie M,Aijaz S,Leefa Chong San IV,Balda MS,Matter K

GEF-H1 mediates tumor necrosis factor-alpha-induced Rho activation and myosin phosphorylation: role in the regulation of tubular paracellular permeability.
The Journal of biological chemistry284(17)11454-66(2009 Apr)
Kakiashvili E,Speight P,Waheed F,Seth R,Lodyga M,Tanimura S,Kohno M,Rotstein OD,Kapus A,Sz?szi K

GEF-H1 mediated control of NOD1 dependent NF-kappaB activation by Shigella effectors.
PLoS pathogens4(11)e1000228(2008 Nov)
Fukazawa A,Alonso C,Kurachi K,Gupta S,Lesser CF,McCormick BA,Reinecker HC

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ARHGEF2 cDNA ORF clone, Canis lupus familiaris(dog)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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