arhgef1a cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	arhgef1a
Entrez Gene ID	368266
Full Name	Rho guanine nucleotide exchange factor (GEF) 1a
Synonyms	arhgef1,cb473,zgc:158261
General protein information	Preferred Names Rho guanine nucleotide exchange factor (GEF) 1a Names rho guanine nucleotide exchange factor 2 rho guanine nucleotide exchange factor (GEF) 1
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001081631.2	NP_001075100.1	rho guanine nucleotide exchange factor 2
XM_009294091.2	XP_009292366.1	rho guanine nucleotide exchange factor 2 isoform X1
XM_009294091.3	XP_009292366.1	rho guanine nucleotide exchange factor 2 isoform X3
XM_021468006.1	XP_021323681.1	rho guanine nucleotide exchange factor 2 isoform X2
XM_021468005.1	XP_021323680.1	rho guanine nucleotide exchange factor 2 isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	ARHGEF2	XP_003308546.1
	ARHGEF2	XP_002801891.1
Bos taurus (cattle)	ARHGEF2	NP_001092351.2
Rattus norvegicus (Norway rat)	Arhgef2	NP_001012079.1
Danio rerio (zebrafish)	arhgef1a	NP_001075100.1
Homo sapiens (human)	ARHGEF2	NP_001155855.1
Canis lupus familiaris (dog)	ARHGEF2	NP_001002995.1
Danio rerio (zebrafish)	arhgef2	XP_689770.5
Xenopus tropicalis (tropical clawed frog)	arhgef2	NP_001001456.1
Mus musculus (house mouse)	Arhgef2	NP_032513.3

Gene Ontology
Bibliography

Related articles in PubMed

Evolution of complexity in the zebrafish synapse proteome.
Bay?s ?, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 Mar)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following arhgef1a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the arhgef1a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa40178	XM_009294091.3 Latest version!	Danio rerio Rho guanine nucleotide exchange factor (GEF) 1a (arhgef1a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
ODa53397	NM_001081631.2 Latest version!	Danio rerio Rho guanine nucleotide exchange factor (GEF) 1a (arhgef1a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
ODa23966	XM_021468005.1 Latest version!	Danio rerio Rho guanine nucleotide exchange factor (GEF) 1a (arhgef1a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
ODa40177	XM_021468006.1 Latest version!	Danio rerio Rho guanine nucleotide exchange factor (GEF) 1a (arhgef1a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
View Old Accession Versions >>
ODa40178	XM_009294091.2	Danio rerio Rho guanine nucleotide exchange factor (GEF) 1a (arhgef1a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

arhgef1a ( XM_009294091.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009292366.1 Danio rerio Rho guanine nucleotide exchange factor (GEF) 1a (arhgef1a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa40178
Clone ID Related Accession (Same CDS sequence)	XM_009294091.2 , XM_009294091.3
Accession Version	XM_009294091.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2625bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	rho guanine nucleotide exchange factor 2 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007130.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009294091.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAGAG TTCAAGAGGG AGAGACAGAT AGGAAAAAAA CTGCCTGTAA CGTCACCATA 
CACAACCGCT GCAGGGACAC ACTACCAAAC TGTGTGAAAA TGAAACAAAA GCAACAAAAA 
ATGGCACTGA TGAGGAACAA TTCAGCCTAT CAGAACGTAA CACTGAGGAA TAAAGCCCAT 
TTAAAGGAAA GGCCGAGTTC TGCCATCTAC CCTTCAGACA GCCTTCGCCA GTCGCTACTC 
GGGTCCCGTC GTGGACGCTC TTCTCTTTTA CTTTCAAAGA GCGTCTCCAC CAATAACATT 
GCAGGGACTC TGAATGATGA CTCTCCTCTG GGGCTGCGCA GGATTTTATC TCAATCCACA 
GACTCACTCA ACTTCAGGAG CAGAACCATG TCTATGGAGT CGCTCAATGA TGAGGGAGAG 
TGCTACGTCT CTTCTCTGCT GGAAGATCTG GATTGTGAAG GCCCAGAATT TGAAGCAGAC 
TCCTGGAGCA TGGCAGTGGA TTCCAGCTAC TTGCAGACCC ACCGTAAAGA TGTTATCAAG 
AGACAGGACG TCATCTACGA GCTGATCCAG ACGGAGCTTC ATCATGTCCG GACGCTGCGG 
ATCATGGACT GCGTGTTCAG ACGGGGGATG CTGGAGGAAG TGCAGCTGGA GCCGGGGGTC 
GTACATGCGC TCTTCCCCTG CCTGGAGCGT CTGCTGAGCA TACACACACA CTTCCTCACA 
CAGCTCCTCG CACGGCGTGC TCACAGCCTG CAGCCCGGCA GCACAAACAA TTTCACCATC 
ACTCAGATCA GCGATGTGCT AATAAAGCAG TTCTCAGGTC AGTTTGCAGA CGATATGCTG 
AAGGCCTATT CTGAGTTCTG CAGTCGCCAT ATGAAGGCTG TCAAACTCTA CAAAGAGCTG 
CTGGCCAGAG ACAAGAGACT TCAGCTCTTT ATACGGAGGG TGAGTCGGGG GCCCTTACTG 
CGCCGTCACG GATTTCAGGA GTGTATCCTG CTGGTCACTC AGCGTATCAC TAAATACCCC 
GTCCTACTGC AGCGAATCCA TGACAACACC AAAGACAATC TGGAGGAAGC TGCAGGCCTG 
TCTCAGGCTC TGGCTTGTCT CCGCGAGCTG CTGTGTTCGG TGGATCAGCA GGTATTGGAG 
TTGGAGAGGA CACATCGGCT GCAGGAGATC CGCTCTCGAG TGGACCCGCG TGCAGAGGCC 
AAACTTCGTT CCGGAGCTCT ATTCCGACCG GCCGAACTGC TCCGCAGGCA GCTTATACAT 
GAAGGCACGC TTCTCTGGAA AACACCCAGC TCTCGACTCA AAGATGTTCA AGTTCTGCTG 
ATGACTGATA TTCTAGTGTT CTTGCAAGAG AAAGATCAGC GGTTTATTTT TGCCAGTTTG 
GACAAGTCTG CTGTAGTGTC ACTGCAGAGT CTATTGGTTC GAGATATAGC CAATCAGGAG 
CGAGGGTTGT TTCTGATCAG CAGTGAGTCC AGCCCACCTG AGATGTACGA GCTCTATGCG 
GCAAGCAAAG ATGACAGAAA CACCTGGCTT CGCCTTATAC AGCAGACCAT CGGCAGCTGT 
CCGTCAAGAG AAGAATTCCC TCTTATAGAA ACAGAAGACA AGGCTCTTCT GCGAAGACTC 
AAAGCTGATA TCCAGCAGAA GGACAGTGAG GTGTTGGAGC TACTACAGGA GCGAGTCACT 
CTGTTCTCAG ACCTGGCGGA GGTCATGGGG GGCTATGAGG TCATGCTGCC AGCCTGCTCC 
AGAAACCTGT TCAGAGCTGA GTCTCCACAG GCTCCTCGAG GAGAACAGCT GCTAACACAG 
GCCATAACAG AGGTGGACAG ATTGACAGAG CTGTTATTAG GATCCGGTTT AGAGGTTCCT 
CTGCCCTGCA ATTCCAACGG CCATCACAAT CCCCCCGGAT CGCTAGTAAT CAATGGACAA 
GAAGTATTAG TTAACGGATC TCAGGAGAAC CAAGCTGCTC AGGACGGGAA TGGGAATCAA 
ATGGAGGACA AGACGCCAAG TGAGGAGGTG TCACAGAGGC TGGTGAATCT CAGTGCTCAG 
TTACACGCTC TTCAGGGTGT GGTGATACGA CAGGACACCA TCTTAGAGCT CTGTCTACGA 
GAGAGCCCCG TCTCCCAGAC CCCCTCAGGA GCTCGCACTG TACGCTCTCT TTCCCGGGAC 
GGAGCCAGTG ACATGGGCTC AGTGGGTGAA CTGGCCCTGC TTCAGCGGCA GCACAGCCTC 
CTGCAGGAGG AGCTGGTGCG CCTCAGAGGA GCCGACGGCA AACTCAGGGA CAGCGAGAGA 
GTCAGAGCTC ATCTGGAGAA ACAGCTCAGG GATATGAAGA CTAATAGCGC CGCTCTAGGG 
GATCGAGGTG GTACTGCAGG CTCACAGGCT CCGTCAAATC GAAGAGAGAG CGATACTGAC 
CCCAACACTG ACACGCCACT GGCCAGCCAG GAATCCATGG ACCAATCGGA CGGCCTGCAG 
GAGTGCAGCG ATGTGGAAGT GGATGTGATT TCGGATGATG ATGACGACGA CGAAGTGGAT 
TCAAGGGTTT CTCCCCGTTC AGAAAGTCCC AGAGATCTGC AGGACATTCC CGAAGAGAGT 
GAAACAGCTA CAGACCCCCG GGAGCGTGAC GCCTCACACT GCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009292366.1
CDS	144..2768
Translation	MERVQEGETDRKKTACNVTIHNRCRDTLPNCVKMKQKQQKMALMRNNSAYQNVTLRNKAHLKERPSSAIYPSDSLRQSLLGSRRGRSSLLLSKSVSTNNIAGTLNDDSPLGLRRILSQSTDSLNFRSRTMSMESLNDEGECYVSSLLEDLDCEGPEFEADSWSMAVDSSYLQTHRKDVIKRQDVIYELIQTELHHVRTLRIMDCVFRRGMLEEVQLEPGVVHALFPCLERLLSIHTHFLTQLLARRAHSLQPGSTNNFTITQISDVLIKQFSGQFADDMLKAYSEFCSRHMKAVKLYKELLARDKRLQLFIRRVSRGPLLRRHGFQECILLVTQRITKYPVLLQRIHDNTKDNLEEAAGLSQALACLRELLCSVDQQVLELERTHRLQEIRSRVDPRAEAKLRSGALFRPAELLRRQLIHEGTLLWKTPSSRLKDVQVLLMTDILVFLQEKDQRFIFASLDKSAVVSLQSLLVRDIANQERGLFLISSESSPPEMYELYAASKDDRNTWLRLIQQTIGSCPSREEFPLIETEDKALLRRLKADIQQKDSEVLELLQERVTLFSDLAEVMGGYEVMLPACSRNLFRAESPQAPRGEQLLTQAITEVDRLTELLLGSGLEVPLPCNSNGHHNPPGSLVINGQEVLVNGSQENQAAQDGNGNQMEDKTPSEEVSQRLVNLSAQLHALQGVVIRQDTILELCLRESPVSQTPSGARTVRSLSRDGASDMGSVGELALLQRQHSLLQEELVRLRGADGKLRDSERVRAHLEKQLRDMKTNSAALGDRGGTAGSQAPSNRRESDTDPNTDTPLASQESMDQSDGLQECSDVEVDVISDDDDDDEVDSRVSPRSESPRDLQDIPEESETATDPRERDASHC

Target ORF information:

RefSeq Version	XM_009294091.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio Rho guanine nucleotide exchange factor (GEF) 1a (arhgef1a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009294091.2

ORF Insert Sequence:

ATGGAGAGAG TTCAAGAGGG AGAGACAGAT AGGAAAAAAA CTGCCTGTAA CGTCACCATA 
CACAACCGCT GCAGGGACAC ACTACCAAAC TGTGTGAAAA TGAAACAAAA GCAACAAAAA 
ATGGCACTGA TGAGGAACAA TTCAGCCTAT CAGAACGTAA CACTGAGGAA TAAAGCCCAT 
TTAAAGGAAA GGCCGAGTTC TGCCATCTAC CCTTCAGACA GCCTTCGCCA GTCGCTACTC 
GGGTCCCGTC GTGGACGCTC TTCTCTTTTA CTTTCAAAGA GCGTCTCCAC CAATAACATT 
GCAGGGACTC TGAATGATGA CTCTCCTCTG GGGCTGCGCA GGATTTTATC TCAATCCACA 
GACTCACTCA ACTTCAGGAG CAGAACCATG TCTATGGAGT CGCTCAATGA TGAGGGAGAG 
TGCTACGTCT CTTCTCTGCT GGAAGATCTG GATTGTGAAG GCCCAGAATT TGAAGCAGAC 
TCCTGGAGCA TGGCAGTGGA TTCCAGCTAC TTGCAGACCC ACCGTAAAGA TGTTATCAAG 
AGACAGGACG TCATCTACGA GCTGATCCAG ACGGAGCTTC ATCATGTCCG GACGCTGCGG 
ATCATGGACT GCGTGTTCAG ACGGGGGATG CTGGAGGAAG TGCAGCTGGA GCCGGGGGTC 
GTACATGCGC TCTTCCCCTG CCTGGAGCGT CTGCTGAGCA TACACACACA CTTCCTCACA 
CAGCTCCTCG CACGGCGTGC TCACAGCCTG CAGCCCGGCA GCACAAACAA TTTCACCATC 
ACTCAGATCA GCGATGTGCT AATAAAGCAG TTCTCAGGTC AGTTTGCAGA CGATATGCTG 
AAGGCCTATT CTGAGTTCTG CAGTCGCCAT ATGAAGGCTG TCAAACTCTA CAAAGAGCTG 
CTGGCCAGAG ACAAGAGACT TCAGCTCTTT ATACGGAGGG TGAGTCGGGG GCCCTTACTG 
CGCCGTCACG GATTTCAGGA GTGTATCCTG CTGGTCACTC AGCGTATCAC TAAATACCCC 
GTCCTACTGC AGCGAATCCA TGACAACACC AAAGACAATC TGGAGGAAGC TGCAGGCCTG 
TCTCAGGCTC TGGCTTGTCT CCGCGAGCTG CTGTGTTCGG TGGATCAGCA GGTATTGGAG 
TTGGAGAGGA CACATCGGCT GCAGGAGATC CGCTCTCGAG TGGACCCGCG TGCAGAGGCC 
AAACTTCGTT CCGGAGCTCT ATTCCGACCG GCCGAACTGC TCCGCAGGCA GCTTATACAT 
GAAGGCACGC TTCTCTGGAA AACACCCAGC TCTCGACTCA AAGATGTTCA AGTTCTGCTG 
ATGACTGATA TTCTAGTGTT CTTGCAAGAG AAAGATCAGC GGTTTATTTT TGCCAGTTTG 
GACAAGTCTG CTGTAGTGTC ACTGCAGAGT CTATTGGTTC GAGATATAGC CAATCAGGAG 
CGAGGGTTGT TTCTGATCAG CAGTGAGTCC AGCCCACCTG AGATGTACGA GCTCTATGCG 
GCAAGCAAAG ATGACAGAAA CACCTGGCTT CGCCTTATAC AGCAGACCAT CGGCAGCTGT 
CCGTCAAGAG AAGAATTCCC TCTTATAGAA ACAGAAGACA AGGCTCTTCT GCGAAGACTC 
AAAGCTGATA TCCAGCAGAA GGACAGTGAG GTGTTGGAGC TACTACAGGA GCGAGTCACT 
CTGTTCTCAG ACCTGGCGGA GGTCATGGGG GGCTATGAGG TCATGCTGCC AGCCTGCTCC 
AGAAACCTGT TCAGAGCTGA GTCTCCACAG GCTCCTCGAG GAGAACAGCT GCTAACACAG 
GCCATAACAG AGGTGGACAG ATTGACAGAG CTGTTATTAG GATCCGGTTT AGAGGTTCCT 
CTGCCCTGCA ATTCCAACGG CCATCACAAT CCCCCCGGAT CGCTAGTAAT CAATGGACAA 
GAAGTATTAG TTAACGGATC TCAGGAGAAC CAAGCTGCTC AGGACGGGAA TGGGAATCAA 
ATGGAGGACA AGACGCCAAG TGAGGAGGTG TCACAGAGGC TGGTGAATCT CAGTGCTCAG 
TTACACGCTC TTCAGGGTGT GGTGATACGA CAGGACACCA TCTTAGAGCT CTGTCTACGA 
GAGAGCCCCG TCTCCCAGAC CCCCTCAGGA GCTCGCACTG TACGCTCTCT TTCCCGGGAC 
GGAGCCAGTG ACATGGGCTC AGTGGGTGAA CTGGCCCTGC TTCAGCGGCA GCACAGCCTC 
CTGCAGGAGG AGCTGGTGCG CCTCAGAGGA GCCGACGGCA AACTCAGGGA CAGCGAGAGA 
GTCAGAGCTC ATCTGGAGAA ACAGCTCAGG GATATGAAGA CTAATAGCGC CGCTCTAGGG 
GATCGAGGTG GTACTGCAGG CTCACAGGCT CCGTCAAATC GAAGAGAGAG CGATACTGAC 
CCCAACACTG ACACGCCACT GGCCAGCCAG GAATCCATGG ACCAATCGGA CGGCCTGCAG 
GAGTGCAGCG ATGTGGAAGT GGATGTGATT TCGGATGATG ATGACGACGA CGAAGTGGAT 
TCAAGGGTTT CTCCCCGTTC AGAAAGTCCC AGAGATCTGC AGGACATTCC CGAAGAGAGT 
GAAACAGCTA CAGACCCCCG GGAGCGTGAC GCCTCACACT GCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

arhgef1a ( XM_009294091.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009292366.1 Danio rerio Rho guanine nucleotide exchange factor (GEF) 1a (arhgef1a), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa40178
Clone ID Related Accession (Same CDS sequence)	XM_009294091.2 , XM_009294091.3
Accession Version	XM_009294091.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2625bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	rho guanine nucleotide exchange factor 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007130.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009294091.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAGAG TTCAAGAGGG AGAGACAGAT AGGAAAAAAA CTGCCTGTAA CGTCACCATA 
CACAACCGCT GCAGGGACAC ACTACCAAAC TGTGTGAAAA TGAAACAAAA GCAACAAAAA 
ATGGCACTGA TGAGGAACAA TTCAGCCTAT CAGAACGTAA CACTGAGGAA TAAAGCCCAT 
TTAAAGGAAA GGCCGAGTTC TGCCATCTAC CCTTCAGACA GCCTTCGCCA GTCGCTACTC 
GGGTCCCGTC GTGGACGCTC TTCTCTTTTA CTTTCAAAGA GCGTCTCCAC CAATAACATT 
GCAGGGACTC TGAATGATGA CTCTCCTCTG GGGCTGCGCA GGATTTTATC TCAATCCACA 
GACTCACTCA ACTTCAGGAG CAGAACCATG TCTATGGAGT CGCTCAATGA TGAGGGAGAG 
TGCTACGTCT CTTCTCTGCT GGAAGATCTG GATTGTGAAG GCCCAGAATT TGAAGCAGAC 
TCCTGGAGCA TGGCAGTGGA TTCCAGCTAC TTGCAGACCC ACCGTAAAGA TGTTATCAAG 
AGACAGGACG TCATCTACGA GCTGATCCAG ACGGAGCTTC ATCATGTCCG GACGCTGCGG 
ATCATGGACT GCGTGTTCAG ACGGGGGATG CTGGAGGAAG TGCAGCTGGA GCCGGGGGTC 
GTACATGCGC TCTTCCCCTG CCTGGAGCGT CTGCTGAGCA TACACACACA CTTCCTCACA 
CAGCTCCTCG CACGGCGTGC TCACAGCCTG CAGCCCGGCA GCACAAACAA TTTCACCATC 
ACTCAGATCA GCGATGTGCT AATAAAGCAG TTCTCAGGTC AGTTTGCAGA CGATATGCTG 
AAGGCCTATT CTGAGTTCTG CAGTCGCCAT ATGAAGGCTG TCAAACTCTA CAAAGAGCTG 
CTGGCCAGAG ACAAGAGACT TCAGCTCTTT ATACGGAGGG TGAGTCGGGG GCCCTTACTG 
CGCCGTCACG GATTTCAGGA GTGTATCCTG CTGGTCACTC AGCGTATCAC TAAATACCCC 
GTCCTACTGC AGCGAATCCA TGACAACACC AAAGACAATC TGGAGGAAGC TGCAGGCCTG 
TCTCAGGCTC TGGCTTGTCT CCGCGAGCTG CTGTGTTCGG TGGATCAGCA GGTATTGGAG 
TTGGAGAGGA CACATCGGCT GCAGGAGATC CGCTCTCGAG TGGACCCGCG TGCAGAGGCC 
AAACTTCGTT CCGGAGCTCT ATTCCGACCG GCCGAACTGC TCCGCAGGCA GCTTATACAT 
GAAGGCACGC TTCTCTGGAA AACACCCAGC TCTCGACTCA AAGATGTTCA AGTTCTGCTG 
ATGACTGATA TTCTAGTGTT CTTGCAAGAG AAAGATCAGC GGTTTATTTT TGCCAGTTTG 
GACAAGTCTG CTGTAGTGTC ACTGCAGAGT CTATTGGTTC GAGATATAGC CAATCAGGAG 
CGAGGGTTGT TTCTGATCAG CAGTGAGTCC AGCCCACCTG AGATGTACGA GCTCTATGCG 
GCAAGCAAAG ATGACAGAAA CACCTGGCTT CGCCTTATAC AGCAGACCAT CGGCAGCTGT 
CCGTCAAGAG AAGAATTCCC TCTTATAGAA ACAGAAGACA AGGCTCTTCT GCGAAGACTC 
AAAGCTGATA TCCAGCAGAA GGACAGTGAG GTGTTGGAGC TACTACAGGA GCGAGTCACT 
CTGTTCTCAG ACCTGGCGGA GGTCATGGGG GGCTATGAGG TCATGCTGCC AGCCTGCTCC 
AGAAACCTGT TCAGAGCTGA GTCTCCACAG GCTCCTCGAG GAGAACAGCT GCTAACACAG 
GCCATAACAG AGGTGGACAG ATTGACAGAG CTGTTATTAG GATCCGGTTT AGAGGTTCCT 
CTGCCCTGCA ATTCCAACGG CCATCACAAT CCCCCCGGAT CGCTAGTAAT CAATGGACAA 
GAAGTATTAG TTAACGGATC TCAGGAGAAC CAAGCTGCTC AGGACGGGAA TGGGAATCAA 
ATGGAGGACA AGACGCCAAG TGAGGAGGTG TCACAGAGGC TGGTGAATCT CAGTGCTCAG 
TTACACGCTC TTCAGGGTGT GGTGATACGA CAGGACACCA TCTTAGAGCT CTGTCTACGA 
GAGAGCCCCG TCTCCCAGAC CCCCTCAGGA GCTCGCACTG TACGCTCTCT TTCCCGGGAC 
GGAGCCAGTG ACATGGGCTC AGTGGGTGAA CTGGCCCTGC TTCAGCGGCA GCACAGCCTC 
CTGCAGGAGG AGCTGGTGCG CCTCAGAGGA GCCGACGGCA AACTCAGGGA CAGCGAGAGA 
GTCAGAGCTC ATCTGGAGAA ACAGCTCAGG GATATGAAGA CTAATAGCGC CGCTCTAGGG 
GATCGAGGTG GTACTGCAGG CTCACAGGCT CCGTCAAATC GAAGAGAGAG CGATACTGAC 
CCCAACACTG ACACGCCACT GGCCAGCCAG GAATCCATGG ACCAATCGGA CGGCCTGCAG 
GAGTGCAGCG ATGTGGAAGT GGATGTGATT TCGGATGATG ATGACGACGA CGAAGTGGAT 
TCAAGGGTTT CTCCCCGTTC AGAAAGTCCC AGAGATCTGC AGGACATTCC CGAAGAGAGT 
GAAACAGCTA CAGACCCCCG GGAGCGTGAC GCCTCACACT GCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009292366.1
CDS	97..2721
Translation	MERVQEGETDRKKTACNVTIHNRCRDTLPNCVKMKQKQQKMALMRNNSAYQNVTLRNKAHLKERPSSAIYPSDSLRQSLLGSRRGRSSLLLSKSVSTNNIAGTLNDDSPLGLRRILSQSTDSLNFRSRTMSMESLNDEGECYVSSLLEDLDCEGPEFEADSWSMAVDSSYLQTHRKDVIKRQDVIYELIQTELHHVRTLRIMDCVFRRGMLEEVQLEPGVVHALFPCLERLLSIHTHFLTQLLARRAHSLQPGSTNNFTITQISDVLIKQFSGQFADDMLKAYSEFCSRHMKAVKLYKELLARDKRLQLFIRRVSRGPLLRRHGFQECILLVTQRITKYPVLLQRIHDNTKDNLEEAAGLSQALACLRELLCSVDQQVLELERTHRLQEIRSRVDPRAEAKLRSGALFRPAELLRRQLIHEGTLLWKTPSSRLKDVQVLLMTDILVFLQEKDQRFIFASLDKSAVVSLQSLLVRDIANQERGLFLISSESSPPEMYELYAASKDDRNTWLRLIQQTIGSCPSREEFPLIETEDKALLRRLKADIQQKDSEVLELLQERVTLFSDLAEVMGGYEVMLPACSRNLFRAESPQAPRGEQLLTQAITEVDRLTELLLGSGLEVPLPCNSNGHHNPPGSLVINGQEVLVNGSQENQAAQDGNGNQMEDKTPSEEVSQRLVNLSAQLHALQGVVIRQDTILELCLRESPVSQTPSGARTVRSLSRDGASDMGSVGELALLQRQHSLLQEELVRLRGADGKLRDSERVRAHLEKQLRDMKTNSAALGDRGGTAGSQAPSNRRESDTDPNTDTPLASQESMDQSDGLQECSDVEVDVISDDDDDDEVDSRVSPRSESPRDLQDIPEESETATDPRERDASHC

Target ORF information:

RefSeq Version	XM_009294091.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio Rho guanine nucleotide exchange factor (GEF) 1a (arhgef1a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009294091.3

ORF Insert Sequence:

ATGGAGAGAG TTCAAGAGGG AGAGACAGAT AGGAAAAAAA CTGCCTGTAA CGTCACCATA 
CACAACCGCT GCAGGGACAC ACTACCAAAC TGTGTGAAAA TGAAACAAAA GCAACAAAAA 
ATGGCACTGA TGAGGAACAA TTCAGCCTAT CAGAACGTAA CACTGAGGAA TAAAGCCCAT 
TTAAAGGAAA GGCCGAGTTC TGCCATCTAC CCTTCAGACA GCCTTCGCCA GTCGCTACTC 
GGGTCCCGTC GTGGACGCTC TTCTCTTTTA CTTTCAAAGA GCGTCTCCAC CAATAACATT 
GCAGGGACTC TGAATGATGA CTCTCCTCTG GGGCTGCGCA GGATTTTATC TCAATCCACA 
GACTCACTCA ACTTCAGGAG CAGAACCATG TCTATGGAGT CGCTCAATGA TGAGGGAGAG 
TGCTACGTCT CTTCTCTGCT GGAAGATCTG GATTGTGAAG GCCCAGAATT TGAAGCAGAC 
TCCTGGAGCA TGGCAGTGGA TTCCAGCTAC TTGCAGACCC ACCGTAAAGA TGTTATCAAG 
AGACAGGACG TCATCTACGA GCTGATCCAG ACGGAGCTTC ATCATGTCCG GACGCTGCGG 
ATCATGGACT GCGTGTTCAG ACGGGGGATG CTGGAGGAAG TGCAGCTGGA GCCGGGGGTC 
GTACATGCGC TCTTCCCCTG CCTGGAGCGT CTGCTGAGCA TACACACACA CTTCCTCACA 
CAGCTCCTCG CACGGCGTGC TCACAGCCTG CAGCCCGGCA GCACAAACAA TTTCACCATC 
ACTCAGATCA GCGATGTGCT AATAAAGCAG TTCTCAGGTC AGTTTGCAGA CGATATGCTG 
AAGGCCTATT CTGAGTTCTG CAGTCGCCAT ATGAAGGCTG TCAAACTCTA CAAAGAGCTG 
CTGGCCAGAG ACAAGAGACT TCAGCTCTTT ATACGGAGGG TGAGTCGGGG GCCCTTACTG 
CGCCGTCACG GATTTCAGGA GTGTATCCTG CTGGTCACTC AGCGTATCAC TAAATACCCC 
GTCCTACTGC AGCGAATCCA TGACAACACC AAAGACAATC TGGAGGAAGC TGCAGGCCTG 
TCTCAGGCTC TGGCTTGTCT CCGCGAGCTG CTGTGTTCGG TGGATCAGCA GGTATTGGAG 
TTGGAGAGGA CACATCGGCT GCAGGAGATC CGCTCTCGAG TGGACCCGCG TGCAGAGGCC 
AAACTTCGTT CCGGAGCTCT ATTCCGACCG GCCGAACTGC TCCGCAGGCA GCTTATACAT 
GAAGGCACGC TTCTCTGGAA AACACCCAGC TCTCGACTCA AAGATGTTCA AGTTCTGCTG 
ATGACTGATA TTCTAGTGTT CTTGCAAGAG AAAGATCAGC GGTTTATTTT TGCCAGTTTG 
GACAAGTCTG CTGTAGTGTC ACTGCAGAGT CTATTGGTTC GAGATATAGC CAATCAGGAG 
CGAGGGTTGT TTCTGATCAG CAGTGAGTCC AGCCCACCTG AGATGTACGA GCTCTATGCG 
GCAAGCAAAG ATGACAGAAA CACCTGGCTT CGCCTTATAC AGCAGACCAT CGGCAGCTGT 
CCGTCAAGAG AAGAATTCCC TCTTATAGAA ACAGAAGACA AGGCTCTTCT GCGAAGACTC 
AAAGCTGATA TCCAGCAGAA GGACAGTGAG GTGTTGGAGC TACTACAGGA GCGAGTCACT 
CTGTTCTCAG ACCTGGCGGA GGTCATGGGG GGCTATGAGG TCATGCTGCC AGCCTGCTCC 
AGAAACCTGT TCAGAGCTGA GTCTCCACAG GCTCCTCGAG GAGAACAGCT GCTAACACAG 
GCCATAACAG AGGTGGACAG ATTGACAGAG CTGTTATTAG GATCCGGTTT AGAGGTTCCT 
CTGCCCTGCA ATTCCAACGG CCATCACAAT CCCCCCGGAT CGCTAGTAAT CAATGGACAA 
GAAGTATTAG TTAACGGATC TCAGGAGAAC CAAGCTGCTC AGGACGGGAA TGGGAATCAA 
ATGGAGGACA AGACGCCAAG TGAGGAGGTG TCACAGAGGC TGGTGAATCT CAGTGCTCAG 
TTACACGCTC TTCAGGGTGT GGTGATACGA CAGGACACCA TCTTAGAGCT CTGTCTACGA 
GAGAGCCCCG TCTCCCAGAC CCCCTCAGGA GCTCGCACTG TACGCTCTCT TTCCCGGGAC 
GGAGCCAGTG ACATGGGCTC AGTGGGTGAA CTGGCCCTGC TTCAGCGGCA GCACAGCCTC 
CTGCAGGAGG AGCTGGTGCG CCTCAGAGGA GCCGACGGCA AACTCAGGGA CAGCGAGAGA 
GTCAGAGCTC ATCTGGAGAA ACAGCTCAGG GATATGAAGA CTAATAGCGC CGCTCTAGGG 
GATCGAGGTG GTACTGCAGG CTCACAGGCT CCGTCAAATC GAAGAGAGAG CGATACTGAC 
CCCAACACTG ACACGCCACT GGCCAGCCAG GAATCCATGG ACCAATCGGA CGGCCTGCAG 
GAGTGCAGCG ATGTGGAAGT GGATGTGATT TCGGATGATG ATGACGACGA CGAAGTGGAT 
TCAAGGGTTT CTCCCCGTTC AGAAAGTCCC AGAGATCTGC AGGACATTCC CGAAGAGAGT 
GAAACAGCTA CAGACCCCCG GGAGCGTGAC GCCTCACACT GCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

arhgef1a ( NM_001081631.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001075100.1 Danio rerio Rho guanine nucleotide exchange factor (GEF) 1a (arhgef1a), mRNA.

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CloneID	ODa53397
Clone ID Related Accession (Same CDS sequence)	NM_001081631.2
Accession Version	NM_001081631.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2808bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-26
Organism	Danio rerio(zebrafish)
Product	rho guanine nucleotide exchange factor 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CT573147.7. On Jun 16, 2016 this sequence version replaced NM_001081631.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: BC133079.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGTCTCGAG TAGCGGATCT GTCGAAACTT CGCCAGGAGA GGATGAGAGA GATCAGTCAG 
CGGAATAAGG AGAAGGAGCG CATGAGGGAG CGGGAGAGAG AAGCGAGAGA GCGTGAAGCA 
CGTTACAGTA ATGGCCACCT CTTCAACTGT CTCACCGTGT CTGGAACCAC TCTCTGCTCG 
GCCTGCAATA AGAGCATCAC CGCTAAGGAG GCCCTCAGCT GCCCCACCTG TAACGTCACC 
ATACACAACC GCTGCAGGGA CACACTACCA AACTGTGTGA AAATGAAACA AAAGCAACAA 
AAAATGGCAC TGATGAGGAA CAATTCAGCC TATCAGAACG TAACACTGAG GAATAAAGCC 
CATTTAAAGG AAAGGCCGAG TTCTGCCATC TACCCTTCAG ACAGCCTTCG CCAGTCGCTA 
CTCGGGTCCC GTCGTGGACG CTCTTCTCTT TTACTTTCAA AGAGCGTCTC CACCAATAAC 
ATTGCAGGGA CTCTGAATGA TGACTCTCCT CTGGGGCTGC GCAGGATTTT ATCTCAATCC 
ACAGACTCAC TCAACTTCAG GAGCAGAACC ATGTCTATGG AGTCGCTCAA TGATGAGGGA 
GAGTGCTACG TCTCTTCTCT GCTGGAAGAT CTGGATTGTG AAGGCCCAGA ATTTGAAGCA 
GACTCCTGGA GCATGGCAGT GGATTCCAGC TACTTGCAGA CCCACCGTAA AGATGTTATC 
AAGAGACAGG ACGTCATCTA CGAGCTGATC CAGACGGAGC TTCATCATGT CCGGACGCTG 
CGGATCATGG ACTGCGTGTT CAGACGGGGG ATGCTGGAGG AAGTGCAGCT GGAGCCGGGG 
GTCGTACATG CGCTCTTCCC CTGCCTGGAG CGTCTGCTGA GCATACACAC ACACTTCCTC 
ACACAGCTCC TCGCACGGCG TGCTCACAGC CTGCAGCCCG GCAGCACAAA CAATTTCACC 
ATCACTCAGA TCAGCGATGT GCTAATAAAG CAGTTCTCAG GTCAGTTTGC AGACGATATG 
CTGAAGGCCT ATTCTGAGTT CTGCAGTCGC CATATGAAGG CTGTCAAACT CTACAAAGAG 
CTGCTGGCCA GAGACAAGAG ACTTCAGCTC TTTATACGGA GGGTGAGTCG GGGGCCCTTA 
CTGCGCCGTC ACGGATTTCA GGAGTGTATC CTGCTGGTCA CTCAGCGTAT CACTAAATAC 
CCCGTCCTAC TGCAGCGAAT CCATGACAAC ACCAAAGACA ATCTGGAGGA AGCTGCAGGC 
CTGTCTCAGG CTCTGGCTTG TCTCCGCGAG CTGCTGTGTT CGGTGGATCA GCAGGTATTG 
GAGTTGGAGA GGACACATCG GCTGCAGGAG ATCCGCTCTC GAGTGGACCC GCGTGCAGAG 
GCCAAACTTC GTTCCGGAGC TCTATTCCGA CCGGCCGAAC TGCTCCGCAG GCAGCTTATA 
CATGAAGGCA CGCTTCTCTG GAAAACACCC AGCTCTCGAC TCAAAGATGT TCAAGTTCTG 
CTGATGACTG ATATTCTAGT GTTCTTGCAA GAGAAAGATC AGCGGTTTAT TTTTGCCAGT 
TTGGACAAGT CTGCTGTAGT GTCACTGCAG AGTCTATTGG TTCGAGATAT AGCCAATCAG 
GAGCGAGGGT TGTTTCTGAT CAGCAGTGAG TCCAGCCCAC CTGAGATGTA CGAGCTCTAT 
GCGGCAAGCA AAGATGACAG AAACACCTGG CTTCGCCTTA TACAGCAGAC CATCGGCAGC 
TGTCCGTCAA GAGAAGAATT CCCTCTTATA GAAACAGAAG ACAAGGCTCT TCTGCGAAGA 
CTCAAAGCTG ATATCCAGCA GAAGGACAGT GAGGTGTTGG AGCTACTACA GGAGCGAGTC 
ACTCTGTTCT CAGACCTGGC GGAGGTCATG GGGGGCTATG AGGTCATGCT GCCAGCCTGC 
TCCAGAAACC TGTTCAGAGC TGAGTCTCCA CAGGCTCCTC GAGGAGAACA GCTGCTAACA 
CAGGCCATAA CAGAGGTGGA CAGATTGACA GAGCTGTTAT TAGGATCCGG TTTAGAGGTT 
CCTCTGCCCT GCAATTCCAA CGGCCATCAC AATCCCCCCG GATCGCTAGT AATCAATGGA 
CAAGAAGTAT TAGTTAACGG ATCTCAGGAG AACCAAGCTG CTCAGGACGG GAATGGGAAT 
CAAATGGAGG ACAAGACGCC AAGTGAGGAG GTGTCACAGA GGCTGGTGAA TCTCAGTGCT 
CAGTTACACG CTCTTCAGGG TGTGGTGATA CGACAGGACA CCATCTTAGA GCTCTGTCTA 
CGAGAGAGCC CCGTCTCCCA GACCCCCTCA GGAGCTCGCA CTGTACGCTC TCTTTCCCGG 
GACGGAGCCA GTGACATGGG CTCAGTGGGT GAACTGGCCC TGCTTCAGCG GCAGCACAGC 
CTCCTGCAGG AGGAGCTGGT GCGCCTCAGA GGAGCCGACG GCAAACTCAG GGACAGCGAG 
AGAGTCAGAG CTCATCTGGA GAAACAGCTC AGGGATATGA AGACTAATAG CGCCGCTCTA 
GGGGATCGAG GTGGTACTGC AGGCTCACAG GCTCCGTCAA ATCGAAGAGA GAGCGATACT 
GACCCCAACA CTGACACGCC ACTGGCCAGC CAGGAATCCA TGGACCAATC GGACGGCCTG 
CAGGAGTGCA GCGATGTGGA AGTGGATGTG ATTTCGGATG ATGATGACGA CGACGAAGTG 
GATTCAAGGG TTTCTCCCCG TTCAGAAAGT CCCAGAGATC TGCAGGACAT TCCCGAAGAG 
AGTGAAACAG CTACAGACCC CCGGGAGCGT GACGCCTCAC ACTGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001075100.1
CDS	165..2972
Translation	MSRVADLSKLRQERMREISQRNKEKERMREREREAREREARYSNGHLFNCLTVSGTTLCSACNKSITAKEALSCPTCNVTIHNRCRDTLPNCVKMKQKQQKMALMRNNSAYQNVTLRNKAHLKERPSSAIYPSDSLRQSLLGSRRGRSSLLLSKSVSTNNIAGTLNDDSPLGLRRILSQSTDSLNFRSRTMSMESLNDEGECYVSSLLEDLDCEGPEFEADSWSMAVDSSYLQTHRKDVIKRQDVIYELIQTELHHVRTLRIMDCVFRRGMLEEVQLEPGVVHALFPCLERLLSIHTHFLTQLLARRAHSLQPGSTNNFTITQISDVLIKQFSGQFADDMLKAYSEFCSRHMKAVKLYKELLARDKRLQLFIRRVSRGPLLRRHGFQECILLVTQRITKYPVLLQRIHDNTKDNLEEAAGLSQALACLRELLCSVDQQVLELERTHRLQEIRSRVDPRAEAKLRSGALFRPAELLRRQLIHEGTLLWKTPSSRLKDVQVLLMTDILVFLQEKDQRFIFASLDKSAVVSLQSLLVRDIANQERGLFLISSESSPPEMYELYAASKDDRNTWLRLIQQTIGSCPSREEFPLIETEDKALLRRLKADIQQKDSEVLELLQERVTLFSDLAEVMGGYEVMLPACSRNLFRAESPQAPRGEQLLTQAITEVDRLTELLLGSGLEVPLPCNSNGHHNPPGSLVINGQEVLVNGSQENQAAQDGNGNQMEDKTPSEEVSQRLVNLSAQLHALQGVVIRQDTILELCLRESPVSQTPSGARTVRSLSRDGASDMGSVGELALLQRQHSLLQEELVRLRGADGKLRDSERVRAHLEKQLRDMKTNSAALGDRGGTAGSQAPSNRRESDTDPNTDTPLASQESMDQSDGLQECSDVEVDVISDDDDDDEVDSRVSPRSESPRDLQDIPEESETATDPRERDASHC

Target ORF information:

RefSeq Version	NM_001081631.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio Rho guanine nucleotide exchange factor (GEF) 1a (arhgef1a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001081631.2

ORF Insert Sequence:

ATGTCTCGAG TAGCGGATCT GTCGAAACTT CGCCAGGAGA GGATGAGAGA GATCAGTCAG 
CGGAATAAGG AGAAGGAGCG CATGAGGGAG CGGGAGAGAG AAGCGAGAGA GCGTGAAGCA 
CGTTACAGTA ATGGCCACCT CTTCAACTGT CTCACCGTGT CTGGAACCAC TCTCTGCTCG 
GCCTGCAATA AGAGCATCAC CGCTAAGGAG GCCCTCAGCT GCCCCACCTG TAACGTCACC 
ATACACAACC GCTGCAGGGA CACACTACCA AACTGTGTGA AAATGAAACA AAAGCAACAA 
AAAATGGCAC TGATGAGGAA CAATTCAGCC TATCAGAACG TAACACTGAG GAATAAAGCC 
CATTTAAAGG AAAGGCCGAG TTCTGCCATC TACCCTTCAG ACAGCCTTCG CCAGTCGCTA 
CTCGGGTCCC GTCGTGGACG CTCTTCTCTT TTACTTTCAA AGAGCGTCTC CACCAATAAC 
ATTGCAGGGA CTCTGAATGA TGACTCTCCT CTGGGGCTGC GCAGGATTTT ATCTCAATCC 
ACAGACTCAC TCAACTTCAG GAGCAGAACC ATGTCTATGG AGTCGCTCAA TGATGAGGGA 
GAGTGCTACG TCTCTTCTCT GCTGGAAGAT CTGGATTGTG AAGGCCCAGA ATTTGAAGCA 
GACTCCTGGA GCATGGCAGT GGATTCCAGC TACTTGCAGA CCCACCGTAA AGATGTTATC 
AAGAGACAGG ACGTCATCTA CGAGCTGATC CAGACGGAGC TTCATCATGT CCGGACGCTG 
CGGATCATGG ACTGCGTGTT CAGACGGGGG ATGCTGGAGG AAGTGCAGCT GGAGCCGGGG 
GTCGTACATG CGCTCTTCCC CTGCCTGGAG CGTCTGCTGA GCATACACAC ACACTTCCTC 
ACACAGCTCC TCGCACGGCG TGCTCACAGC CTGCAGCCCG GCAGCACAAA CAATTTCACC 
ATCACTCAGA TCAGCGATGT GCTAATAAAG CAGTTCTCAG GTCAGTTTGC AGACGATATG 
CTGAAGGCCT ATTCTGAGTT CTGCAGTCGC CATATGAAGG CTGTCAAACT CTACAAAGAG 
CTGCTGGCCA GAGACAAGAG ACTTCAGCTC TTTATACGGA GGGTGAGTCG GGGGCCCTTA 
CTGCGCCGTC ACGGATTTCA GGAGTGTATC CTGCTGGTCA CTCAGCGTAT CACTAAATAC 
CCCGTCCTAC TGCAGCGAAT CCATGACAAC ACCAAAGACA ATCTGGAGGA AGCTGCAGGC 
CTGTCTCAGG CTCTGGCTTG TCTCCGCGAG CTGCTGTGTT CGGTGGATCA GCAGGTATTG 
GAGTTGGAGA GGACACATCG GCTGCAGGAG ATCCGCTCTC GAGTGGACCC GCGTGCAGAG 
GCCAAACTTC GTTCCGGAGC TCTATTCCGA CCGGCCGAAC TGCTCCGCAG GCAGCTTATA 
CATGAAGGCA CGCTTCTCTG GAAAACACCC AGCTCTCGAC TCAAAGATGT TCAAGTTCTG 
CTGATGACTG ATATTCTAGT GTTCTTGCAA GAGAAAGATC AGCGGTTTAT TTTTGCCAGT 
TTGGACAAGT CTGCTGTAGT GTCACTGCAG AGTCTATTGG TTCGAGATAT AGCCAATCAG 
GAGCGAGGGT TGTTTCTGAT CAGCAGTGAG TCCAGCCCAC CTGAGATGTA CGAGCTCTAT 
GCGGCAAGCA AAGATGACAG AAACACCTGG CTTCGCCTTA TACAGCAGAC CATCGGCAGC 
TGTCCGTCAA GAGAAGAATT CCCTCTTATA GAAACAGAAG ACAAGGCTCT TCTGCGAAGA 
CTCAAAGCTG ATATCCAGCA GAAGGACAGT GAGGTGTTGG AGCTACTACA GGAGCGAGTC 
ACTCTGTTCT CAGACCTGGC GGAGGTCATG GGGGGCTATG AGGTCATGCT GCCAGCCTGC 
TCCAGAAACC TGTTCAGAGC TGAGTCTCCA CAGGCTCCTC GAGGAGAACA GCTGCTAACA 
CAGGCCATAA CAGAGGTGGA CAGATTGACA GAGCTGTTAT TAGGATCCGG TTTAGAGGTT 
CCTCTGCCCT GCAATTCCAA CGGCCATCAC AATCCCCCCG GATCGCTAGT AATCAATGGA 
CAAGAAGTAT TAGTTAACGG ATCTCAGGAG AACCAAGCTG CTCAGGACGG GAATGGGAAT 
CAAATGGAGG ACAAGACGCC AAGTGAGGAG GTGTCACAGA GGCTGGTGAA TCTCAGTGCT 
CAGTTACACG CTCTTCAGGG TGTGGTGATA CGACAGGACA CCATCTTAGA GCTCTGTCTA 
CGAGAGAGCC CCGTCTCCCA GACCCCCTCA GGAGCTCGCA CTGTACGCTC TCTTTCCCGG 
GACGGAGCCA GTGACATGGG CTCAGTGGGT GAACTGGCCC TGCTTCAGCG GCAGCACAGC 
CTCCTGCAGG AGGAGCTGGT GCGCCTCAGA GGAGCCGACG GCAAACTCAG GGACAGCGAG 
AGAGTCAGAG CTCATCTGGA GAAACAGCTC AGGGATATGA AGACTAATAG CGCCGCTCTA 
GGGGATCGAG GTGGTACTGC AGGCTCACAG GCTCCGTCAA ATCGAAGAGA GAGCGATACT 
GACCCCAACA CTGACACGCC ACTGGCCAGC CAGGAATCCA TGGACCAATC GGACGGCCTG 
CAGGAGTGCA GCGATGTGGA AGTGGATGTG ATTTCGGATG ATGATGACGA CGACGAAGTG 
GATTCAAGGG TTTCTCCCCG TTCAGAAAGT CCCAGAGATC TGCAGGACAT TCCCGAAGAG 
AGTGAAACAG CTACAGACCC CCGGGAGCGT GACGCCTCAC ACTGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

arhgef1a ( XM_021468005.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021323680.1 Danio rerio Rho guanine nucleotide exchange factor (GEF) 1a (arhgef1a), transcript variant X1, mRNA.

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CloneID	ODa23966
Clone ID Related Accession (Same CDS sequence)	XM_021468005.1
Accession Version	XM_021468005.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3450bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	rho guanine nucleotide exchange factor 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007130.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCCCTG AGGATCTGCC TGACCTTATG GACACGATGT CGAGCTCTCC TCCTCCCTCT 
CCTCGTCCTT CATGTTTGTC GTTCTCCCCT TCTCACTGCT CTCCACCGAT GTCTATCGAC 
CCGGCTCATG TGACCCTACG CTCCCCTGTT ACTCGTCACC TGACTTCAAT CGACCCCTGG 
AGGAGACACA GCTGGGAGCC TGGAGCCGTG GCGCAGGGGT ATCCACCCAA TGACACACGC 
AGTGTGAGTC TGGAAGATCT GGACCCGGAT GAGATGTCCT TAGTGCTGAA TGGTGCTTTA 
AGGTCCAAAA GAGCCCGTGA AGTGCGCAGG TCTGTTACTC AAGCCTCCTT GACATCACTC 
ACGGAGGAGG AAGTTGGAAC AGAGCATCAG GATCATCATC GATCTGCGCT GGAGGAGCAG 
TCCAATCAGA TGCATGGATG CAGTGCCTCC GCCCCCAGTC TGTGTATGAT GCAACAGATG 
CCCAGAAGCT CCGCCCCTCG CCCACGTTCC TACTGCCATG AGAGTAATTT GCACCACCAG 
ATTGGAGGAT CTTCCAACAG TATAGACAGT GAGTATGCAG CTCTGGGTTT GGATACAGAT 
AGAAGACAAG TAAAGGCAAA GAGGCAAGAA AACAAAGAGG ATCTGTCAGG AAATCGTCTG 
GAGCGAACAC TAAGCTTCCT GCGCAAGATG ACTGCTAATC GCAAGAATAA GGAGAAGGAG 
CGCATGAGGG AGCGGGAGAG AGAAGCGAGA GAGCGTGAAG CACGTTACAG TAATGGCCAC 
CTCTTCAACT GTCTCACCGT GTCTGGAACC ACTCTCTGCT CGGCCTGCAA TAAGAGCATC 
ACCGCTAAGG AGGCCCTCAG CTGCCCCACC TGTAACGTCA CCATACACAA CCGCTGCAGG 
GACACACTAC CAAACTGTGT GAAAATGAAA CAAAAGCAAC AAAAAATGGC ACTGATGAGG 
AACAATTCAG CCTATCAGAA CGTAACACTG AGGAATAAAG CCCATTTAAA GGAAAGGCCG 
AGTTCTGCCA TCTACCCTTC AGACAGCCTT CGCCAGTCGC TACTCGGGTC CCGTCGTGGA 
CGCTCTTCTC TTTTACTTTC AAAGAGCGTC TCCACCAATA ACATTGCAGG GACTCTGAAT 
GATGACTCTC CTCTGGGGCT GCGCAGGATT TTATCTCAAT CCACAGACTC ACTCAACTTC 
AGGAGCAGAA CCATGTCTAT GGAGTCGCTC AATGATGAGG GAGAGTGCTA CGTCTCTTCT 
CTGCTGGAAG ATCTGGATTG TGAAGGCCCA GAATTTGAAG CAGACTCCTG GAGCATGGCA 
GTGGATTCCA GCTACTTGCA GACCCACCGT AAAGATGTTA TCAAGAGACA GGACGTCATC 
TACGAGCTGA TCCAGACGGA GCTTCATCAT GTCCGGACGC TGCGGATCAT GGACTGCGTG 
TTCAGACGGG GGATGCTGGA GGAAGTGCAG CTGGAGCCGG GGGTCGTACA TGCGCTCTTC 
CCCTGCCTGG AGCGTCTGCT GAGCATACAC ACACACTTCC TCACACAGCT CCTCGCACGG 
CGTGCTCACA GCCTGCAGCC CGGCAGCACA AACAATTTCA CCATCACTCA GATCAGCGAT 
GTGCTAATAA AGCAGTTCTC AGGTCAGTTT GCAGACGATA TGCTGAAGGC CTATTCTGAG 
TTCTGCAGTC GCCATATGAA GGCTGTCAAA CTCTACAAAG AGCTGCTGGC CAGAGACAAG 
AGACTTCAGC TCTTTATACG GAGGGTGAGT CGGGGGCCCT TACTGCGCCG TCACGGATTT 
CAGGAGTGTA TCCTGCTGGT CACTCAGCGT ATCACTAAAT ACCCCGTCCT ACTGCAGCGA 
ATCCATGACA ACACCAAAGA CAATCTGGAG GAAGCTGCAG GCCTGTCTCA GGCTCTGGCT 
TGTCTCCGCG AGCTGCTGTG TTCGGTGGAT CAGCAGGTAT TGGAGTTGGA GAGGACACAT 
CGGCTGCAGG AGATCCGCTC TCGAGTGGAC CCGCGTGCAG AGGCCAAACT TCGTTCCGGA 
GCTCTATTCC GACCGGCCGA ACTGCTCCGC AGGCAGCTTA TACATGAAGG CACGCTTCTC 
TGGAAAACAC CCAGCTCTCG ACTCAAAGAT GTTCAAGTTC TGCTGATGAC TGATATTCTA 
GTGTTCTTGC AAGAGAAAGA TCAGCGGTTT ATTTTTGCCA GTTTGGACAA GTCTGCTGTA 
GTGTCACTGC AGAGTCTATT GGTTCGAGAT ATAGCCAATC AGGAGCGAGG GTTGTTTCTG 
ATCAGCAGTG AGTCCAGCCC ACCTGAGATG TACGAGCTCT ATGCGGCAAG CAAAGATGAC 
AGAAACACCT GGCTTCGCCT TATACAGCAG ACCATCGGCA GCTGTCCGTC AAGAGAAGAA 
TTCCCTCTTA TAGAAACAGA AGACAAGGCT CTTCTGCGAA GACTCAAAGC TGATATCCAG 
CAGAAGGACA GTGAGGTGTT GGAGCTACTA CAGGAGCGAG TCACTCTGTT CTCAGACCTG 
GCGGAGGTCA TGGGGGGCTA TGAGGTCATG CTGCCAGCCT GCTCCAGAAA CCTGTTCAGA 
GCTGAGTCTC CACAGGCTCC TCGAGGAGAA CAGCTGCTAA CACAGGCCAT AACAGAGGTG 
GACAGATTGA CAGAGCTGTT ATTAGGATCC GGTTTAGAGG TTCCTCTGCC CTGCAATTCC 
AACGGCCATC ACAATCCCCC CGGATCGCTA GTAATCAATG GACAAGAAGT ATTAGTTAAC 
GGATCTCAGG AGAACCAAGC TGCTCAGGAC GGGAATGGGA ATCAAATGGA GGACAAGACG 
CCAAGTGAGG AGGTGTCACA GAGGCTGGTG AATCTCAGTG CTCAGTTACA CGCTCTTCAG 
GGTGTGGTGA TACGACAGGA CACCATCTTA GAGCTCTGTC TACGAGAGAG CCCCGTCTCC 
CAGACCCCCT CAGGAGCTCG CACTGTACGC TCTCTTTCCC GGGACGGAGC CAGTGACATG 
GGCTCAGTGG GTGAACTGGC CCTGCTTCAG CGGCAGCACA GCCTCCTGCA GGAGGAGCTG 
GTGCGCCTCA GAGGAGCCGA CGGCAAACTC AGGGACAGCG AGAGAGTCAG AGCTCATCTG 
GAGAAACAGC TCAGGGATAT GAAGACTAAT AGCGCCGCTC TAGGGGATCG AGGTGGTACT 
GCAGGCTCAC AGGCTCCGTC AAATCGAAGA GAGAGCGATA CTGACCCCAA CACTGACACG 
CCACTGGCCA GCCAGGAATC CATGGACCAA TCGGACGGCC TGCAGGAGTG CAGCGATGTG 
GAAGTGGATG TGATTTCGGA TGATGATGAC GACGACGAAG TGGATTCAAG GGTTTCTCCC 
CGTTCAGAAA GTCCCAGAGA TCTGCAGGAC ATTCCCGAAG AGAGTGAAAC AGCTACAGAC 
CCCCGGGAGC GTGACGCCTC ACACTGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021323680.1
CDS	272..3721
Translation	MGPEDLPDLMDTMSSSPPPSPRPSCLSFSPSHCSPPMSIDPAHVTLRSPVTRHLTSIDPWRRHSWEPGAVAQGYPPNDTRSVSLEDLDPDEMSLVLNGALRSKRAREVRRSVTQASLTSLTEEEVGTEHQDHHRSALEEQSNQMHGCSASAPSLCMMQQMPRSSAPRPRSYCHESNLHHQIGGSSNSIDSEYAALGLDTDRRQVKAKRQENKEDLSGNRLERTLSFLRKMTANRKNKEKERMREREREAREREARYSNGHLFNCLTVSGTTLCSACNKSITAKEALSCPTCNVTIHNRCRDTLPNCVKMKQKQQKMALMRNNSAYQNVTLRNKAHLKERPSSAIYPSDSLRQSLLGSRRGRSSLLLSKSVSTNNIAGTLNDDSPLGLRRILSQSTDSLNFRSRTMSMESLNDEGECYVSSLLEDLDCEGPEFEADSWSMAVDSSYLQTHRKDVIKRQDVIYELIQTELHHVRTLRIMDCVFRRGMLEEVQLEPGVVHALFPCLERLLSIHTHFLTQLLARRAHSLQPGSTNNFTITQISDVLIKQFSGQFADDMLKAYSEFCSRHMKAVKLYKELLARDKRLQLFIRRVSRGPLLRRHGFQECILLVTQRITKYPVLLQRIHDNTKDNLEEAAGLSQALACLRELLCSVDQQVLELERTHRLQEIRSRVDPRAEAKLRSGALFRPAELLRRQLIHEGTLLWKTPSSRLKDVQVLLMTDILVFLQEKDQRFIFASLDKSAVVSLQSLLVRDIANQERGLFLISSESSPPEMYELYAASKDDRNTWLRLIQQTIGSCPSREEFPLIETEDKALLRRLKADIQQKDSEVLELLQERVTLFSDLAEVMGGYEVMLPACSRNLFRAESPQAPRGEQLLTQAITEVDRLTELLLGSGLEVPLPCNSNGHHNPPGSLVINGQEVLVNGSQENQAAQDGNGNQMEDKTPSEEVSQRLVNLSAQLHALQGVVIRQDTILELCLRESPVSQTPSGARTVRSLSRDGASDMGSVGELALLQRQHSLLQEELVRLRGADGKLRDSERVRAHLEKQLRDMKTNSAALGDRGGTAGSQAPSNRRESDTDPNTDTPLASQESMDQSDGLQECSDVEVDVISDDDDDDEVDSRVSPRSESPRDLQDIPEESETATDPRERDASHC

Target ORF information:

RefSeq Version	XM_021468005.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio Rho guanine nucleotide exchange factor (GEF) 1a (arhgef1a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021468005.1

ORF Insert Sequence:

ATGGGCCCTG AGGATCTGCC TGACCTTATG GACACGATGT CGAGCTCTCC TCCTCCCTCT 
CCTCGTCCTT CATGTTTGTC GTTCTCCCCT TCTCACTGCT CTCCACCGAT GTCTATCGAC 
CCGGCTCATG TGACCCTACG CTCCCCTGTT ACTCGTCACC TGACTTCAAT CGACCCCTGG 
AGGAGACACA GCTGGGAGCC TGGAGCCGTG GCGCAGGGGT ATCCACCCAA TGACACACGC 
AGTGTGAGTC TGGAAGATCT GGACCCGGAT GAGATGTCCT TAGTGCTGAA TGGTGCTTTA 
AGGTCCAAAA GAGCCCGTGA AGTGCGCAGG TCTGTTACTC AAGCCTCCTT GACATCACTC 
ACGGAGGAGG AAGTTGGAAC AGAGCATCAG GATCATCATC GATCTGCGCT GGAGGAGCAG 
TCCAATCAGA TGCATGGATG CAGTGCCTCC GCCCCCAGTC TGTGTATGAT GCAACAGATG 
CCCAGAAGCT CCGCCCCTCG CCCACGTTCC TACTGCCATG AGAGTAATTT GCACCACCAG 
ATTGGAGGAT CTTCCAACAG TATAGACAGT GAGTATGCAG CTCTGGGTTT GGATACAGAT 
AGAAGACAAG TAAAGGCAAA GAGGCAAGAA AACAAAGAGG ATCTGTCAGG AAATCGTCTG 
GAGCGAACAC TAAGCTTCCT GCGCAAGATG ACTGCTAATC GCAAGAATAA GGAGAAGGAG 
CGCATGAGGG AGCGGGAGAG AGAAGCGAGA GAGCGTGAAG CACGTTACAG TAATGGCCAC 
CTCTTCAACT GTCTCACCGT GTCTGGAACC ACTCTCTGCT CGGCCTGCAA TAAGAGCATC 
ACCGCTAAGG AGGCCCTCAG CTGCCCCACC TGTAACGTCA CCATACACAA CCGCTGCAGG 
GACACACTAC CAAACTGTGT GAAAATGAAA CAAAAGCAAC AAAAAATGGC ACTGATGAGG 
AACAATTCAG CCTATCAGAA CGTAACACTG AGGAATAAAG CCCATTTAAA GGAAAGGCCG 
AGTTCTGCCA TCTACCCTTC AGACAGCCTT CGCCAGTCGC TACTCGGGTC CCGTCGTGGA 
CGCTCTTCTC TTTTACTTTC AAAGAGCGTC TCCACCAATA ACATTGCAGG GACTCTGAAT 
GATGACTCTC CTCTGGGGCT GCGCAGGATT TTATCTCAAT CCACAGACTC ACTCAACTTC 
AGGAGCAGAA CCATGTCTAT GGAGTCGCTC AATGATGAGG GAGAGTGCTA CGTCTCTTCT 
CTGCTGGAAG ATCTGGATTG TGAAGGCCCA GAATTTGAAG CAGACTCCTG GAGCATGGCA 
GTGGATTCCA GCTACTTGCA GACCCACCGT AAAGATGTTA TCAAGAGACA GGACGTCATC 
TACGAGCTGA TCCAGACGGA GCTTCATCAT GTCCGGACGC TGCGGATCAT GGACTGCGTG 
TTCAGACGGG GGATGCTGGA GGAAGTGCAG CTGGAGCCGG GGGTCGTACA TGCGCTCTTC 
CCCTGCCTGG AGCGTCTGCT GAGCATACAC ACACACTTCC TCACACAGCT CCTCGCACGG 
CGTGCTCACA GCCTGCAGCC CGGCAGCACA AACAATTTCA CCATCACTCA GATCAGCGAT 
GTGCTAATAA AGCAGTTCTC AGGTCAGTTT GCAGACGATA TGCTGAAGGC CTATTCTGAG 
TTCTGCAGTC GCCATATGAA GGCTGTCAAA CTCTACAAAG AGCTGCTGGC CAGAGACAAG 
AGACTTCAGC TCTTTATACG GAGGGTGAGT CGGGGGCCCT TACTGCGCCG TCACGGATTT 
CAGGAGTGTA TCCTGCTGGT CACTCAGCGT ATCACTAAAT ACCCCGTCCT ACTGCAGCGA 
ATCCATGACA ACACCAAAGA CAATCTGGAG GAAGCTGCAG GCCTGTCTCA GGCTCTGGCT 
TGTCTCCGCG AGCTGCTGTG TTCGGTGGAT CAGCAGGTAT TGGAGTTGGA GAGGACACAT 
CGGCTGCAGG AGATCCGCTC TCGAGTGGAC CCGCGTGCAG AGGCCAAACT TCGTTCCGGA 
GCTCTATTCC GACCGGCCGA ACTGCTCCGC AGGCAGCTTA TACATGAAGG CACGCTTCTC 
TGGAAAACAC CCAGCTCTCG ACTCAAAGAT GTTCAAGTTC TGCTGATGAC TGATATTCTA 
GTGTTCTTGC AAGAGAAAGA TCAGCGGTTT ATTTTTGCCA GTTTGGACAA GTCTGCTGTA 
GTGTCACTGC AGAGTCTATT GGTTCGAGAT ATAGCCAATC AGGAGCGAGG GTTGTTTCTG 
ATCAGCAGTG AGTCCAGCCC ACCTGAGATG TACGAGCTCT ATGCGGCAAG CAAAGATGAC 
AGAAACACCT GGCTTCGCCT TATACAGCAG ACCATCGGCA GCTGTCCGTC AAGAGAAGAA 
TTCCCTCTTA TAGAAACAGA AGACAAGGCT CTTCTGCGAA GACTCAAAGC TGATATCCAG 
CAGAAGGACA GTGAGGTGTT GGAGCTACTA CAGGAGCGAG TCACTCTGTT CTCAGACCTG 
GCGGAGGTCA TGGGGGGCTA TGAGGTCATG CTGCCAGCCT GCTCCAGAAA CCTGTTCAGA 
GCTGAGTCTC CACAGGCTCC TCGAGGAGAA CAGCTGCTAA CACAGGCCAT AACAGAGGTG 
GACAGATTGA CAGAGCTGTT ATTAGGATCC GGTTTAGAGG TTCCTCTGCC CTGCAATTCC 
AACGGCCATC ACAATCCCCC CGGATCGCTA GTAATCAATG GACAAGAAGT ATTAGTTAAC 
GGATCTCAGG AGAACCAAGC TGCTCAGGAC GGGAATGGGA ATCAAATGGA GGACAAGACG 
CCAAGTGAGG AGGTGTCACA GAGGCTGGTG AATCTCAGTG CTCAGTTACA CGCTCTTCAG 
GGTGTGGTGA TACGACAGGA CACCATCTTA GAGCTCTGTC TACGAGAGAG CCCCGTCTCC 
CAGACCCCCT CAGGAGCTCG CACTGTACGC TCTCTTTCCC GGGACGGAGC CAGTGACATG 
GGCTCAGTGG GTGAACTGGC CCTGCTTCAG CGGCAGCACA GCCTCCTGCA GGAGGAGCTG 
GTGCGCCTCA GAGGAGCCGA CGGCAAACTC AGGGACAGCG AGAGAGTCAG AGCTCATCTG 
GAGAAACAGC TCAGGGATAT GAAGACTAAT AGCGCCGCTC TAGGGGATCG AGGTGGTACT 
GCAGGCTCAC AGGCTCCGTC AAATCGAAGA GAGAGCGATA CTGACCCCAA CACTGACACG 
CCACTGGCCA GCCAGGAATC CATGGACCAA TCGGACGGCC TGCAGGAGTG CAGCGATGTG 
GAAGTGGATG TGATTTCGGA TGATGATGAC GACGACGAAG TGGATTCAAG GGTTTCTCCC 
CGTTCAGAAA GTCCCAGAGA TCTGCAGGAC ATTCCCGAAG AGAGTGAAAC AGCTACAGAC 
CCCCGGGAGC GTGACGCCTC ACACTGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

arhgef1a ( XM_021468006.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021323681.1 Danio rerio Rho guanine nucleotide exchange factor (GEF) 1a (arhgef1a), transcript variant X2, mRNA.

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CloneID	ODa40177
Clone ID Related Accession (Same CDS sequence)	XM_021468006.1
Accession Version	XM_021468006.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3420bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	rho guanine nucleotide exchange factor 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007130.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCCCTG AGGATCTGCC TGACCTTATG GACACGATGT CGAGCTCTCC TCCTCCCTCT 
CCTCGTCCTT CATGTTTGTC GTTCTCCCCT TCTCACTGCT CTCCACCGAT GTCTATCGAC 
CCGGCTCATG TGACCCTACG CTCCCCTGTT ACTCGTCACC TGACTTCAAT CGACCCCTGG 
AGGAGACACA GCTGGGAGCC TGGAGCCGTG GCGCAGGGGT ATCCACCCAA TGACACACGC 
AGTGTGAGTC TGGAAGATCT GGACCCGGAT GAGATGTCCT TAGTGCTGAA TGGTGCTTTA 
AGGTCCAAAA GAGCCCGTGA AGTGCGCAGG TCTGTTACTC AAGCCTCCTT GACATCACTC 
ACGGAGGAGG AAGTTGGAAC AGAGCATCAG GATCATCATC GATCTGCGCT GGAGGAGCAG 
TCCAATCAGA TGCATGGATG CAGTGCCTCC GCCCCCAGTC TGTGTATGAT GCAACAGATG 
CCCAGAAGCT CCGCCCCTCG CCCACGTTCC TACTGCCATG AGAGTAATTT GCACCACCAG 
ATTGGAGGAT CTTCCAACAG TATAGACAGT GAGTATGCAG CTCTGGGTTT GGATACAGAT 
AGAAGACAAG TAAAGGCAAA GAGGCAAGAA AACAAAGAGG ATCTGTCAGG AAATCGTCTG 
GAGCGAACAC TAAGCTTCCT GCGCAAGATG ACTGCTAATC GCAAGAATAA GGAGAAGGAG 
CGCATGAGGG AGCGGGAGAG AGAAGCGAGA GAGCGTGAAG CACGTTACAG TAATGGCCAC 
CTCTTCAACT GTCTCACCGT GTCTGGAACC ACTCTCTGCT CGGCCTGCAA TAAGAGCATC 
ACCGCTAAGG AGGCCCTCAG CTGCCCCACC TGTAACGTCA CCATACACAA CCGCTGCAGG 
GACACACTAC CAAACTGTGT GAAAATGAAA CAAAAGCAAC AAAAAATGGC ACTGATGAGG 
AACAATTCAG CCTATCAGAA CGTAACACTG AGGAATAAAG CCCATTTAAA GGAAAGGCCG 
AGTTCTGCCA TCTACCCTTC AGACAGCCTT CGCCAGTCGC TACTCGGGTC CCGTCGTGGA 
CGCTCTTCTC TTTTACTTTC AAAGAGCGTC TCCACCAATA ACATTGCAGG TTTAATGATT 
TTATCTCAAT CCACAGACTC ACTCAACTTC AGGAGCAGAA CCATGTCTAT GGAGTCGCTC 
AATGATGAGG GAGAGTGCTA CGTCTCTTCT CTGCTGGAAG ATCTGGATTG TGAAGGCCCA 
GAATTTGAAG CAGACTCCTG GAGCATGGCA GTGGATTCCA GCTACTTGCA GACCCACCGT 
AAAGATGTTA TCAAGAGACA GGACGTCATC TACGAGCTGA TCCAGACGGA GCTTCATCAT 
GTCCGGACGC TGCGGATCAT GGACTGCGTG TTCAGACGGG GGATGCTGGA GGAAGTGCAG 
CTGGAGCCGG GGGTCGTACA TGCGCTCTTC CCCTGCCTGG AGCGTCTGCT GAGCATACAC 
ACACACTTCC TCACACAGCT CCTCGCACGG CGTGCTCACA GCCTGCAGCC CGGCAGCACA 
AACAATTTCA CCATCACTCA GATCAGCGAT GTGCTAATAA AGCAGTTCTC AGGTCAGTTT 
GCAGACGATA TGCTGAAGGC CTATTCTGAG TTCTGCAGTC GCCATATGAA GGCTGTCAAA 
CTCTACAAAG AGCTGCTGGC CAGAGACAAG AGACTTCAGC TCTTTATACG GAGGGTGAGT 
CGGGGGCCCT TACTGCGCCG TCACGGATTT CAGGAGTGTA TCCTGCTGGT CACTCAGCGT 
ATCACTAAAT ACCCCGTCCT ACTGCAGCGA ATCCATGACA ACACCAAAGA CAATCTGGAG 
GAAGCTGCAG GCCTGTCTCA GGCTCTGGCT TGTCTCCGCG AGCTGCTGTG TTCGGTGGAT 
CAGCAGGTAT TGGAGTTGGA GAGGACACAT CGGCTGCAGG AGATCCGCTC TCGAGTGGAC 
CCGCGTGCAG AGGCCAAACT TCGTTCCGGA GCTCTATTCC GACCGGCCGA ACTGCTCCGC 
AGGCAGCTTA TACATGAAGG CACGCTTCTC TGGAAAACAC CCAGCTCTCG ACTCAAAGAT 
GTTCAAGTTC TGCTGATGAC TGATATTCTA GTGTTCTTGC AAGAGAAAGA TCAGCGGTTT 
ATTTTTGCCA GTTTGGACAA GTCTGCTGTA GTGTCACTGC AGAGTCTATT GGTTCGAGAT 
ATAGCCAATC AGGAGCGAGG GTTGTTTCTG ATCAGCAGTG AGTCCAGCCC ACCTGAGATG 
TACGAGCTCT ATGCGGCAAG CAAAGATGAC AGAAACACCT GGCTTCGCCT TATACAGCAG 
ACCATCGGCA GCTGTCCGTC AAGAGAAGAA TTCCCTCTTA TAGAAACAGA AGACAAGGCT 
CTTCTGCGAA GACTCAAAGC TGATATCCAG CAGAAGGACA GTGAGGTGTT GGAGCTACTA 
CAGGAGCGAG TCACTCTGTT CTCAGACCTG GCGGAGGTCA TGGGGGGCTA TGAGGTCATG 
CTGCCAGCCT GCTCCAGAAA CCTGTTCAGA GCTGAGTCTC CACAGGCTCC TCGAGGAGAA 
CAGCTGCTAA CACAGGCCAT AACAGAGGTG GACAGATTGA CAGAGCTGTT ATTAGGATCC 
GGTTTAGAGG TTCCTCTGCC CTGCAATTCC AACGGCCATC ACAATCCCCC CGGATCGCTA 
GTAATCAATG GACAAGAAGT ATTAGTTAAC GGATCTCAGG AGAACCAAGC TGCTCAGGAC 
GGGAATGGGA ATCAAATGGA GGACAAGACG CCAAGTGAGG AGGTGTCACA GAGGCTGGTG 
AATCTCAGTG CTCAGTTACA CGCTCTTCAG GGTGTGGTGA TACGACAGGA CACCATCTTA 
GAGCTCTGTC TACGAGAGAG CCCCGTCTCC CAGACCCCCT CAGGAGCTCG CACTGTACGC 
TCTCTTTCCC GGGACGGAGC CAGTGACATG GGCTCAGTGG GTGAACTGGC CCTGCTTCAG 
CGGCAGCACA GCCTCCTGCA GGAGGAGCTG GTGCGCCTCA GAGGAGCCGA CGGCAAACTC 
AGGGACAGCG AGAGAGTCAG AGCTCATCTG GAGAAACAGC TCAGGGATAT GAAGACTAAT 
AGCGCCGCTC TAGGGGATCG AGGTGGTACT GCAGGCTCAC AGGCTCCGTC AAATCGAAGA 
GAGAGCGATA CTGACCCCAA CACTGACACG CCACTGGCCA GCCAGGAATC CATGGACCAA 
TCGGACGGCC TGCAGGAGTG CAGCGATGTG GAAGTGGATG TGATTTCGGA TGATGATGAC 
GACGACGAAG TGGATTCAAG GGTTTCTCCC CGTTCAGAAA GTCCCAGAGA TCTGCAGGAC 
ATTCCCGAAG AGAGTGAAAC AGCTACAGAC CCCCGGGAGC GTGACGCCTC ACACTGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021323681.1
CDS	272..3691
Translation	MGPEDLPDLMDTMSSSPPPSPRPSCLSFSPSHCSPPMSIDPAHVTLRSPVTRHLTSIDPWRRHSWEPGAVAQGYPPNDTRSVSLEDLDPDEMSLVLNGALRSKRAREVRRSVTQASLTSLTEEEVGTEHQDHHRSALEEQSNQMHGCSASAPSLCMMQQMPRSSAPRPRSYCHESNLHHQIGGSSNSIDSEYAALGLDTDRRQVKAKRQENKEDLSGNRLERTLSFLRKMTANRKNKEKERMREREREAREREARYSNGHLFNCLTVSGTTLCSACNKSITAKEALSCPTCNVTIHNRCRDTLPNCVKMKQKQQKMALMRNNSAYQNVTLRNKAHLKERPSSAIYPSDSLRQSLLGSRRGRSSLLLSKSVSTNNIAGLMILSQSTDSLNFRSRTMSMESLNDEGECYVSSLLEDLDCEGPEFEADSWSMAVDSSYLQTHRKDVIKRQDVIYELIQTELHHVRTLRIMDCVFRRGMLEEVQLEPGVVHALFPCLERLLSIHTHFLTQLLARRAHSLQPGSTNNFTITQISDVLIKQFSGQFADDMLKAYSEFCSRHMKAVKLYKELLARDKRLQLFIRRVSRGPLLRRHGFQECILLVTQRITKYPVLLQRIHDNTKDNLEEAAGLSQALACLRELLCSVDQQVLELERTHRLQEIRSRVDPRAEAKLRSGALFRPAELLRRQLIHEGTLLWKTPSSRLKDVQVLLMTDILVFLQEKDQRFIFASLDKSAVVSLQSLLVRDIANQERGLFLISSESSPPEMYELYAASKDDRNTWLRLIQQTIGSCPSREEFPLIETEDKALLRRLKADIQQKDSEVLELLQERVTLFSDLAEVMGGYEVMLPACSRNLFRAESPQAPRGEQLLTQAITEVDRLTELLLGSGLEVPLPCNSNGHHNPPGSLVINGQEVLVNGSQENQAAQDGNGNQMEDKTPSEEVSQRLVNLSAQLHALQGVVIRQDTILELCLRESPVSQTPSGARTVRSLSRDGASDMGSVGELALLQRQHSLLQEELVRLRGADGKLRDSERVRAHLEKQLRDMKTNSAALGDRGGTAGSQAPSNRRESDTDPNTDTPLASQESMDQSDGLQECSDVEVDVISDDDDDDEVDSRVSPRSESPRDLQDIPEESETATDPRERDASHC

Target ORF information:

RefSeq Version	XM_021468006.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio Rho guanine nucleotide exchange factor (GEF) 1a (arhgef1a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021468006.1

ORF Insert Sequence:

ATGGGCCCTG AGGATCTGCC TGACCTTATG GACACGATGT CGAGCTCTCC TCCTCCCTCT 
CCTCGTCCTT CATGTTTGTC GTTCTCCCCT TCTCACTGCT CTCCACCGAT GTCTATCGAC 
CCGGCTCATG TGACCCTACG CTCCCCTGTT ACTCGTCACC TGACTTCAAT CGACCCCTGG 
AGGAGACACA GCTGGGAGCC TGGAGCCGTG GCGCAGGGGT ATCCACCCAA TGACACACGC 
AGTGTGAGTC TGGAAGATCT GGACCCGGAT GAGATGTCCT TAGTGCTGAA TGGTGCTTTA 
AGGTCCAAAA GAGCCCGTGA AGTGCGCAGG TCTGTTACTC AAGCCTCCTT GACATCACTC 
ACGGAGGAGG AAGTTGGAAC AGAGCATCAG GATCATCATC GATCTGCGCT GGAGGAGCAG 
TCCAATCAGA TGCATGGATG CAGTGCCTCC GCCCCCAGTC TGTGTATGAT GCAACAGATG 
CCCAGAAGCT CCGCCCCTCG CCCACGTTCC TACTGCCATG AGAGTAATTT GCACCACCAG 
ATTGGAGGAT CTTCCAACAG TATAGACAGT GAGTATGCAG CTCTGGGTTT GGATACAGAT 
AGAAGACAAG TAAAGGCAAA GAGGCAAGAA AACAAAGAGG ATCTGTCAGG AAATCGTCTG 
GAGCGAACAC TAAGCTTCCT GCGCAAGATG ACTGCTAATC GCAAGAATAA GGAGAAGGAG 
CGCATGAGGG AGCGGGAGAG AGAAGCGAGA GAGCGTGAAG CACGTTACAG TAATGGCCAC 
CTCTTCAACT GTCTCACCGT GTCTGGAACC ACTCTCTGCT CGGCCTGCAA TAAGAGCATC 
ACCGCTAAGG AGGCCCTCAG CTGCCCCACC TGTAACGTCA CCATACACAA CCGCTGCAGG 
GACACACTAC CAAACTGTGT GAAAATGAAA CAAAAGCAAC AAAAAATGGC ACTGATGAGG 
AACAATTCAG CCTATCAGAA CGTAACACTG AGGAATAAAG CCCATTTAAA GGAAAGGCCG 
AGTTCTGCCA TCTACCCTTC AGACAGCCTT CGCCAGTCGC TACTCGGGTC CCGTCGTGGA 
CGCTCTTCTC TTTTACTTTC AAAGAGCGTC TCCACCAATA ACATTGCAGG TTTAATGATT 
TTATCTCAAT CCACAGACTC ACTCAACTTC AGGAGCAGAA CCATGTCTAT GGAGTCGCTC 
AATGATGAGG GAGAGTGCTA CGTCTCTTCT CTGCTGGAAG ATCTGGATTG TGAAGGCCCA 
GAATTTGAAG CAGACTCCTG GAGCATGGCA GTGGATTCCA GCTACTTGCA GACCCACCGT 
AAAGATGTTA TCAAGAGACA GGACGTCATC TACGAGCTGA TCCAGACGGA GCTTCATCAT 
GTCCGGACGC TGCGGATCAT GGACTGCGTG TTCAGACGGG GGATGCTGGA GGAAGTGCAG 
CTGGAGCCGG GGGTCGTACA TGCGCTCTTC CCCTGCCTGG AGCGTCTGCT GAGCATACAC 
ACACACTTCC TCACACAGCT CCTCGCACGG CGTGCTCACA GCCTGCAGCC CGGCAGCACA 
AACAATTTCA CCATCACTCA GATCAGCGAT GTGCTAATAA AGCAGTTCTC AGGTCAGTTT 
GCAGACGATA TGCTGAAGGC CTATTCTGAG TTCTGCAGTC GCCATATGAA GGCTGTCAAA 
CTCTACAAAG AGCTGCTGGC CAGAGACAAG AGACTTCAGC TCTTTATACG GAGGGTGAGT 
CGGGGGCCCT TACTGCGCCG TCACGGATTT CAGGAGTGTA TCCTGCTGGT CACTCAGCGT 
ATCACTAAAT ACCCCGTCCT ACTGCAGCGA ATCCATGACA ACACCAAAGA CAATCTGGAG 
GAAGCTGCAG GCCTGTCTCA GGCTCTGGCT TGTCTCCGCG AGCTGCTGTG TTCGGTGGAT 
CAGCAGGTAT TGGAGTTGGA GAGGACACAT CGGCTGCAGG AGATCCGCTC TCGAGTGGAC 
CCGCGTGCAG AGGCCAAACT TCGTTCCGGA GCTCTATTCC GACCGGCCGA ACTGCTCCGC 
AGGCAGCTTA TACATGAAGG CACGCTTCTC TGGAAAACAC CCAGCTCTCG ACTCAAAGAT 
GTTCAAGTTC TGCTGATGAC TGATATTCTA GTGTTCTTGC AAGAGAAAGA TCAGCGGTTT 
ATTTTTGCCA GTTTGGACAA GTCTGCTGTA GTGTCACTGC AGAGTCTATT GGTTCGAGAT 
ATAGCCAATC AGGAGCGAGG GTTGTTTCTG ATCAGCAGTG AGTCCAGCCC ACCTGAGATG 
TACGAGCTCT ATGCGGCAAG CAAAGATGAC AGAAACACCT GGCTTCGCCT TATACAGCAG 
ACCATCGGCA GCTGTCCGTC AAGAGAAGAA TTCCCTCTTA TAGAAACAGA AGACAAGGCT 
CTTCTGCGAA GACTCAAAGC TGATATCCAG CAGAAGGACA GTGAGGTGTT GGAGCTACTA 
CAGGAGCGAG TCACTCTGTT CTCAGACCTG GCGGAGGTCA TGGGGGGCTA TGAGGTCATG 
CTGCCAGCCT GCTCCAGAAA CCTGTTCAGA GCTGAGTCTC CACAGGCTCC TCGAGGAGAA 
CAGCTGCTAA CACAGGCCAT AACAGAGGTG GACAGATTGA CAGAGCTGTT ATTAGGATCC 
GGTTTAGAGG TTCCTCTGCC CTGCAATTCC AACGGCCATC ACAATCCCCC CGGATCGCTA 
GTAATCAATG GACAAGAAGT ATTAGTTAAC GGATCTCAGG AGAACCAAGC TGCTCAGGAC 
GGGAATGGGA ATCAAATGGA GGACAAGACG CCAAGTGAGG AGGTGTCACA GAGGCTGGTG 
AATCTCAGTG CTCAGTTACA CGCTCTTCAG GGTGTGGTGA TACGACAGGA CACCATCTTA 
GAGCTCTGTC TACGAGAGAG CCCCGTCTCC CAGACCCCCT CAGGAGCTCG CACTGTACGC 
TCTCTTTCCC GGGACGGAGC CAGTGACATG GGCTCAGTGG GTGAACTGGC CCTGCTTCAG 
CGGCAGCACA GCCTCCTGCA GGAGGAGCTG GTGCGCCTCA GAGGAGCCGA CGGCAAACTC 
AGGGACAGCG AGAGAGTCAG AGCTCATCTG GAGAAACAGC TCAGGGATAT GAAGACTAAT 
AGCGCCGCTC TAGGGGATCG AGGTGGTACT GCAGGCTCAC AGGCTCCGTC AAATCGAAGA 
GAGAGCGATA CTGACCCCAA CACTGACACG CCACTGGCCA GCCAGGAATC CATGGACCAA 
TCGGACGGCC TGCAGGAGTG CAGCGATGTG GAAGTGGATG TGATTTCGGA TGATGATGAC 
GACGACGAAG TGGATTCAAG GGTTTCTCCC CGTTCAGAAA GTCCCAGAGA TCTGCAGGAC 
ATTCCCGAAG AGAGTGAAAC AGCTACAGAC CCCCGGGAGC GTGACGCCTC ACACTGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 Mar)
Bay?s ?,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

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arhgef1a cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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