trp-4 cDNA ORF clone, Caenorhabditis elegans

Gene
Clones
gRNAs

Summary

Gene Symbol	trp-4
Entrez Gene ID	190574
Full Name	TRP (transient receptor potential) channel family
Gene Type	protein-coding
Organism	Caenorhabditis elegans

mRNA and Protein(s)

mRNA	Protein	Name
NM_061028.2	NP_493429.2	TRP (transient receptor potential) channel family
NM_061028.3	NP_493429.2	ANK_REP_REGION domain-containing protein

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	trpn1	NP_899192.1
Caenorhabditis elegans (roundworm)	trp-4	NP_493429.2
Xenopus tropicalis (tropical clawed frog)	LOC100492885	XP_002934518.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008559	XP_314669.4
Drosophila melanogaster (fruit fly)	nompC	NP_001097089.2

Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.

Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Neuron-specific misexpression of TRP-4, the pore-forming subunit of a mechanotransduction channel, sensitizes neurons to ultrasound stimulus, resulting in behavioural outputs.
Title: Sonogenetics is a non-invasive approach to activating neurons in Caenorhabditis elegans.

TRP-4 functions as an essential pore-forming subunit of a native mechanotransduction channel.
Title: C. elegans TRP family protein TRP-4 is a pore-forming subunit of a native mechanotransduction channel.

TRP-4 acts in a single neuron, DVA, to mediate its function in proprioception, and that the activity of DVA can be stimulated by body stretch
Title: A C. elegans stretch receptor neuron revealed by a mechanosensitive TRP channel homologue.

The following trp-4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the trp-4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa335587	NM_061028.3 Latest version!	Caenorhabditis elegans ANK_REP_REGION domain-containing protein (trp-4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
View Old Accession Versions >>
OCa335587	NM_061028.2	Caenorhabditis elegans TRP (transient receptor potential) channel family (trp-4), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

trp-4 ( NM_061028.2 ) cDNA ORF clone, Caenorhabditis elegans -> NP_493429.2 Caenorhabditis elegans TRP (transient receptor potential) channel family (trp-4), partial mRNA.

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CloneID	OCa335587
Clone ID Related Accession (Same CDS sequence)	NM_061028.2 , NM_061028.3
Accession Version	NM_061028.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5775bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	TRP (transient receptor potential) channel family
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003279). On Nov 13, 2008 this sequence version replaced NM_061028.1. COMPLETENESS: incomplete on both ends.

ATGGATTCGC CACGTGGCGG AATCCTGGGA AGAGCTTTAC GAGAAGCATC ACAATCGACT 
AGGCAAGAAA ATGATGTTGA TATGGATCAG GTACCCGTAC GGCAGATGAA CAGGGATTAC 
GGTGGATCCA GGAGGACTCA GATGAATCCC CACACCTCCC AACCTGGTCC ATCTCATGTA 
TCAATTGTAA ATGTCCCAGA ACGCGGAGGA CCCACATCTT CCACATCAAC CACACATGAG 
ACAGAGCACA CGGCACATAG GACAGAGTCC GGGAGGTTTA TCAGACGCCG TCGCCAATCT 
CGAGAGGTTA CCACCACAAC CACAAGACCC TATGACCCCG CTCCTCCAAC CCAGACCCGA 
ACAAGCTCCG GCTCAACAGT AAATGGATGG GGGGAGAATC GACCGAAATC TGCTGATGAG 
GAGATCAAAC GGCGGCGAAG AAGTGGCGGG GGAATCCTGT CTCGCGGGCT TCGAGAAATG 
AACAAAATGG TGGAAGAGTT GGAGCAAGCA AGTGAAGAGC CAAGTACCAG GAAGGGAATT 
CTGGGTACTG CGTTAAAGGA TATGGAAGGG ACCACTTATC AAAAGATTTA CAGGAAAAGG 
GAGGAAACTC CCAAGCGCTC CCGTTCATTT GACGATCAGG AGATGTCGAA TCGAGTAGGA 
ATGATCGAGC ACTTGCTCCG AGACAAGGAT CCTTTGGAGC TTCAGCAGTT GGGATTAACC 
GACCTCCTCA CCACCGACAC CATCCCAACT GACCGACCAC CCCTCCGCCG ATCCTCGACC 
CATCTCCAAA TCGGAAAGAA CTCACGGATC ATCTTCGTTC CGAAACAACC ATCCCGTGAT 
TCAGTCACCC CGCCGGATCG TCTTCTCGGG AAACCTCTGT TTCGAGAGAG TCTCACCTCC 
CACGCATCGT CTCATGAGGA AATGTCGAGT GAGGACTTGG CAATGGCGGA TCCTCAGACG 
AAGATTTTGT ATTTCGCGAA GAGAGATGAG TGGGCGAATG TGGAGTCTGA GATAGAGACT 
ATCAAGCGGA GTGATTTTAG TATGGCTGAT AATCACGGCT TCACCGCCTT CCTCCTAGCC 
GTCAAAGCTG GCAAGGATCA AATCGTAGAC AAGATGATCC GAAAAGGTGC TCGAGTGGAC 
TATAGCACTA AAGATGGCCG TAACGCGACT CATATTGCCG CCATGTACTC CGGAGTTGAA 
ACTCTTGAGC TTATCCTCAA GCGATACTCT GAGCTGCTCC GAAAAGGTGC GGGGCCTAAA 
AAGCAGCTGG CAATCCATGT GGCTTGCGAG AGAAAATCCA AGAAAGCATT TCCAATTGTG 
AAGCGGATTT TGGAAGATAC TGATCAAAGA ATGGCAGAGG ATGGGGATGG ATCCTTGCCG 
ATACACTTGG CATTCAAGTT TGGGAATGTT AATATTGTGG AGCTTCTGCT AAGTGGGCCT 
TCGGATGAAC AAACCAGGAA AGCTGATGGA AACGGGGATA CCTTGCTTCA TTTGGCCGCT 
CGGAGTGGGA ATATCGAAGC GGTTCGGACA GCGATTGCGG CTGGATGTGA TAATGCGAAT 
GTGCAGAATA GGGTGGGAAG GACGCCGCTA CATGAGGTAG CCGAAGTCGG AGATCAAAAT 
ATGCTAAAAA TCATGTTCAA ACTCCGCGCC GACGCCAACA TCCATGATAA GGAGGACAAG 
ACTCCGGTAC ACGTTGCAGC GGAGCGAGGT GACACTTCGA TGGTCGAGTC ACTAATTGAC 
AAGTTTGGTG GCTCAATTCG CGCTAGGACC CGTGATGGGT CGACGCTTCT GCATATTGCC 
GCATGTTCAG GACATACTAG CACCGCATTG GCGTTTTTGA AGAGAGGAGT CCCCCTCTTC 
ATGCCCAACA AAAAAGGAGC CCTGGGTCTT CACTCCGCAG CAGCTGCTGG CTTCAACGAC 
GTCGTCAAAA TGCTCATTGC TCGGGGTACT AATGTAGATG TCCGTACACG AGACAACTAC 
ACCGCTCTCC ACGTAGCGGT TCAATCTGGC AAGGCTTCGG TTGTAGAGAC CCTGCTGGGA 
AGTGGTGCAG ACATTCATGT GAAGGGCGGG GAACTAGGAC AAACTGCACT GCACATTGCG 
GCAAGCTTGA ATGGAGCCGA GAGTCGGGAT TGTGCGATGA TGTTGCTGAA AAGTGGAGGG 
CAGCCGGATG TTGCACAAAT GGATGGGGAG ACTTGTCTGC ATATTGCTGC CAGGAGTGGG 
AATAAGGATA TCATGAGGCT CCTGCTTGAC GAGAACGCCG ACTCGAAAAT AAGCTCAAAG 
ATCGGAGAGA CACCCCTCCA GGTGGCCGCC AAGTCATGCA ATTTTGAAGC AGCATCAATG 
ATTTTGAAGC ACCTTTCGGA AGTTCTGACC CAAGAACAGC TTAAGGAACA TGTCAATCAT 
AGAACCAATG ACGGCTTCAC AGCTCTTCAC TACGCCGCTG AAATCGAGCA GCGCCAGTTA 
CACTTTCCAG GAGAAGATGC CAAGCTAGTA AATCTTCTGA TCGACTACGG TGGAATGGTA 
GAAATGCCAT CACTCAATGC AAATGAGACG GCGATGCATA TGGCGGCAAG ATCCGGAAAT 
CAAGCTGTAC TCCTGGCGAT GGTCAATAAG ATCGGAGCCG GTGCGGTGCA AATCGTGCAG 
AACAAGCAGA GCAAGAACGG ATGGTCACCG CTGTTGGAAG CATGTGCCAG AGGGCATTCT 
GGAGTGGCGA ATATTTTGTT GAAGCACCAC GCCCGTATTG ATGTATTCGA TGAAATGGGC 
CGTACTGCTC TGCACCTGGC AGCTTTCAAT GGGCATCTCT CCCTGGTTCA CCTTCTTCTG 
CAGCACAAAG CATTCGTGAA CAGTAAATCG AAAACCGGAG AGGCACCGCT CCACTTAGCA 
GCTCAGCATG GTCATGTGAA GGTGGTGAAT GTCCTGGTGC AGGATCATGG TGCAGCGCTG 
GAGGCAATTA CGCTGGATAA TCAGACAGCC CTCCACTTTG CCGCAAAATT CGGTCAGCTA 
GCTGTGAGTC AAACCCTTCT GGCTCTCGGA GCAAACCCCA ATGCACGTGA CGACAAGGGT 
CAAACCCCTC TCCATCTGGC AGCTGAGAAT GACTTCCCCG ACGTTGTGAA GCTCTTCCTG 
AAAATGAGAA ATAACAACCG GAGTGTGTTG ACCGCAATTG ATCATAATGG ATTCACCTGC 
GCACATATTG CTGCGATGAA GGGTTCCCTA GCCGTGGTCC GTGAGCTTAT GATGATCGAC 
AAGCCTATGG TAATCCAGGC AAAGACCAAA ACACTGGAAG CCACTACACT TCATATGGCA 
GCTGCGGGAG GTCACGCGAA CATTGTGAAG ATTCTGCTGG AGAATGGAGC AAACGCGGAA 
GATGAGAATT CGCACGGAAT GACTGCTCTC CACCTTGGCG CCAAAAACGG ATTCATATCG 
ATTTTGGAGG CATTCGATAA GATCCTATGG AAACGGTGTT CGAGAAAGAC CGGTCTCAAC 
GCTCTCCACA TCGCTGCGTT CTACGGAAAT TCGGATTTCG TCAATGAAAT GCTCAAGCAC 
GTACAAGCAA CAGTCCGTTC CGAGCCGCCC ATCTACAATC ACCATGTCAA TAAGGAATTC 
TCAACTGAAT ACGGCTTCAC ACCTCTCCAT TTAGCCGCTC AAAGTGGACA CGACAGTCTT 
GTGCGGATGC TTCTGAATCA GGGAGTGCAA GTTGACGCGA CCAGTACTAC AATGAACGTG 
ATCCCCCTCC ATCTGGCTGC CCAGCAAGGC CACATCGCAG TGGTAGGAAT GCTCCTGTCC 
AGATCTACTC AGCAGCAGCA CGCCAAGGAT TGGAGAGGCA GGACCCCGCT CCACCTAGCC 
GCTCAGAATG GCCACTACGA GATGGTCTCA CTTCTCATTG CTCAGGGATC TAACATCAAT 
GTCATGGATC AGAATGGCTG GACTGGTCTT CACTTTGCCA CTCGTGCCGG GCACCTGAGT 
GTCGTCAAGC TGTTCATCGA TAGTTCAGCG GATCCATTGG CGGAGACCAA GGAGGGCAAA 
GTTCCATTGT GCTTTGCTGC AGCTCATAAT CATATAGAAT GTCTTCGATT CCTCCTGAAA 
CAGAAGCATG ACACACATCA ATTGATGGAA GATCGGAAGT TCATATTCGA CTTGATGGTT 
TGTGGTAAAA CCAATGACAA TGAGCCTCTA CAAGAGTTTA TTCTTCAATC ACCTGCTCCA 
ATTGAGACGG CAGTCAAGTT GTCCGCGTTG TACAGAGATA TGTCGGAGAA GGAGAAGGAG 
AGGGCGAAGG ATCTGTTGAA TGTGGCAGTG TTCAGTGAGA ATATGGCTGT GGAGTTGTTA 
GGGATCACCG CCACCGAATA CAATGCCGCT CTTCTCCTGA AGGCTAAGGA CAATCGAGGC 
CGGCCCCTAC TAGATGTTCT CATTGAAAAT GAGCAGAAAG AAGTAGTCTC CTACGCGTCT 
GTCCAACGCT ACCTGACAGA AGTATGGACT GCCCGTGTCG ACTGGTCATT CGGAAAGTTT 
GTCGCATTCT CCCTCTTCGT GCTAATATGC CCCCCGGCAT GGTTCTACTT CTCACTTCCA 
CTGGATAGTC GGATCGGAAG AGCTCCGATT ATTAAATTTG TGTGCCATAT CGTGTCTCAT 
GTCTATTTTA CGATACTGCT GACAATTGTG GTGTTGAATA TTACACATAA GATGTACGAA 
GTAACTTCGG TGGTTCCAAA CCCTGTGGAA TGGCTCCTGT TGCTCTGGCT CTCTGGAAAT 
CTGGTCTCCG AACTCTCCAC TGTCGGTGGA GGATCTGGCC TAGGAATCGT AAAGGTCCTA 
ATCCTAGTCC TTTCCGCGAT GGCGATAGCC GTCCATGTCC TAGCCTTCCT GCTCCCGGCA 
GTATTCCTAA CCCACCTGGA TAACGATGAA AAGCTACATT TCGCCCGGAC AATGCTTTAT 
TTGAAAAATC AACTTTTCGC CTTTGCCCTG CTATTTGCTT TTGTAGAGTA CCTGGATTTC 
CTGACAGTGC ATCATTTGTT CGGTCCCTGG GCGATCATTA TTCGAGATCT AATGTATGAT 
TTGGCCCGTT TCCTTGTGAT CCTGATGTTG TTCGTGGCGG GCTTCACACT CCACGTGACG 
AGTATCTTCC AGCCTGCCTA CCAGCCTGTC GACGAGGACA GCGCCGAGCT GATGCGTCTG 
GCCTCCCCGT CTCAAACCCT CGAAATGCTC TTCTTCTCGC TCTTCGGACT CGTCGAGCCC 
GATTCAATGC CCCCGCTCCA TCTAGTTCCA GATTTTGCAA AAATCATCTT AAAACTTCTA 
TTCGGAATCT ACATGATGGT CACCTTGATT GTGCTGATCA ACTTGCTGAT TGCTATGATG 
TCTGACACCT ACCAACGAAT TCAGGCACAG TCGGATAAGG AATGGAAGTT CGGAAGAGCT 
ATTCTGATCA GACAGATGAA TAAGAAAAGC GCCACGCCGT CGCCGATAAA TATGTTAACA 
AAGTTGATAA TTGTGCTGAG GGTAGCCTGG CGGAATCGGT TGAGATGCAT GACCCGAAAA 
GCCCAAGACG ATCTTCGCTT CGAGGAGAAC ATCGACGCGT TCTCCATGGG TGGCGGCCAG 
CAGGGAAGGC AAAGTCCGAC CAATGAAGGA AGAGAAGGCC AGCAAGAGCT TGGTAACTCG 
GCTGACTGGA ACATCGAGAC AGTCATCGAC TGGAGGAAGA TTGTTTCAAT GTACTATCAG 
GCGAATGGGA AGCTTACAGA CGGGCGAACC AAAGAGGATG TGGATTTGGC AATGGCAGTA 
CCTACTAGTT TTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_493429.2
CDS	1..5775
Translation	MDSPRGGILGRALREASQSTRQENDVDMDQVPVRQMNRDYGGSRRTQMNPHTSQPGPSHVSIVNVPERGGPTSSTSTTHETEHTAHRTESGRFIRRRRQSREVTTTTTRPYDPAPPTQTRTSSGSTVNGWGENRPKSADEEIKRRRRSGGGILSRGLREMNKMVEELEQASEEPSTRKGILGTALKDMEGTTYQKIYRKREETPKRSRSFDDQEMSNRVGMIEHLLRDKDPLELQQLGLTDLLTTDTIPTDRPPLRRSSTHLQIGKNSRIIFVPKQPSRDSVTPPDRLLGKPLFRESLTSHASSHEEMSSEDLAMADPQTKILYFAKRDEWANVESEIETIKRSDFSMADNHGFTAFLLAVKAGKDQIVDKMIRKGARVDYSTKDGRNATHIAAMYSGVETLELILKRYSELLRKGAGPKKQLAIHVACERKSKKAFPIVKRILEDTDQRMAEDGDGSLPIHLAFKFGNVNIVELLLSGPSDEQTRKADGNGDTLLHLAARSGNIEAVRTAIAAGCDNANVQNRVGRTPLHEVAEVGDQNMLKIMFKLRADANIHDKEDKTPVHVAAERGDTSMVESLIDKFGGSIRARTRDGSTLLHIAACSGHTSTALAFLKRGVPLFMPNKKGALGLHSAAAAGFNDVVKMLIARGTNVDVRTRDNYTALHVAVQSGKASVVETLLGSGADIHVKGGELGQTALHIAASLNGAESRDCAMMLLKSGGQPDVAQMDGETCLHIAARSGNKDIMRLLLDENADSKISSKIGETPLQVAAKSCNFEAASMILKHLSEVLTQEQLKEHVNHRTNDGFTALHYAAEIEQRQLHFPGEDAKLVNLLIDYGGMVEMPSLNANETAMHMAARSGNQAVLLAMVNKIGAGAVQIVQNKQSKNGWSPLLEACARGHSGVANILLKHHARIDVFDEMGRTALHLAAFNGHLSLVHLLLQHKAFVNSKSKTGEAPLHLAAQHGHVKVVNVLVQDHGAALEAITLDNQTALHFAAKFGQLAVSQTLLALGANPNARDDKGQTPLHLAAENDFPDVVKLFLKMRNNNRSVLTAIDHNGFTCAHIAAMKGSLAVVRELMMIDKPMVIQAKTKTLEATTLHMAAAGGHANIVKILLENGANAEDENSHGMTALHLGAKNGFISILEAFDKILWKRCSRKTGLNALHIAAFYGNSDFVNEMLKHVQATVRSEPPIYNHHVNKEFSTEYGFTPLHLAAQSGHDSLVRMLLNQGVQVDATSTTMNVIPLHLAAQQGHIAVVGMLLSRSTQQQHAKDWRGRTPLHLAAQNGHYEMVSLLIAQGSNINVMDQNGWTGLHFATRAGHLSVVKLFIDSSADPLAETKEGKVPLCFAAAHNHIECLRFLLKQKHDTHQLMEDRKFIFDLMVCGKTNDNEPLQEFILQSPAPIETAVKLSALYRDMSEKEKERAKDLLNVAVFSENMAVELLGITATEYNAALLLKAKDNRGRPLLDVLIENEQKEVVSYASVQRYLTEVWTARVDWSFGKFVAFSLFVLICPPAWFYFSLPLDSRIGRAPIIKFVCHIVSHVYFTILLTIVVLNITHKMYEVTSVVPNPVEWLLLLWLSGNLVSELSTVGGGSGLGIVKVLILVLSAMAIAVHVLAFLLPAVFLTHLDNDEKLHFARTMLYLKNQLFAFALLFAFVEYLDFLTVHHLFGPWAIIIRDLMYDLARFLVILMLFVAGFTLHVTSIFQPAYQPVDEDSAELMRLASPSQTLEMLFFSLFGLVEPDSMPPLHLVPDFAKIILKLLFGIYMMVTLIVLINLLIAMMSDTYQRIQAQSDKEWKFGRAILIRQMNKKSATPSPINMLTKLIIVLRVAWRNRLRCMTRKAQDDLRFEENIDAFSMGGGQQGRQSPTNEGREGQQELGNSADWNIETVIDWRKIVSMYYQANGKLTDGRTKEDVDLAMAVPTSF

Target ORF information:

RefSeq Version	NM_061028.2
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans TRP (transient receptor potential) channel family (trp-4), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_061028.2

ORF Insert Sequence:

ATGGATTCGC CACGTGGCGG AATCCTGGGA AGAGCTTTAC GAGAAGCATC ACAATCGACT 
AGGCAAGAAA ATGATGTTGA TATGGATCAG GTACCCGTAC GGCAGATGAA CAGGGATTAC 
GGTGGATCCA GGAGGACTCA GATGAATCCC CACACCTCCC AACCTGGTCC ATCTCATGTA 
TCAATTGTAA ATGTCCCAGA ACGCGGAGGA CCCACATCTT CCACATCAAC CACACATGAG 
ACAGAGCACA CGGCACATAG GACAGAGTCC GGGAGGTTTA TCAGACGCCG TCGCCAATCT 
CGAGAGGTTA CCACCACAAC CACAAGACCC TATGACCCCG CTCCTCCAAC CCAGACCCGA 
ACAAGCTCCG GCTCAACAGT AAATGGATGG GGGGAGAATC GACCGAAATC TGCTGATGAG 
GAGATCAAAC GGCGGCGAAG AAGTGGCGGG GGAATCCTGT CTCGCGGGCT TCGAGAAATG 
AACAAAATGG TGGAAGAGTT GGAGCAAGCA AGTGAAGAGC CAAGTACCAG GAAGGGAATT 
CTGGGTACTG CGTTAAAGGA TATGGAAGGG ACCACTTATC AAAAGATTTA CAGGAAAAGG 
GAGGAAACTC CCAAGCGCTC CCGTTCATTT GACGATCAGG AGATGTCGAA TCGAGTAGGA 
ATGATCGAGC ACTTGCTCCG AGACAAGGAT CCTTTGGAGC TTCAGCAGTT GGGATTAACC 
GACCTCCTCA CCACCGACAC CATCCCAACT GACCGACCAC CCCTCCGCCG ATCCTCGACC 
CATCTCCAAA TCGGAAAGAA CTCACGGATC ATCTTCGTTC CGAAACAACC ATCCCGTGAT 
TCAGTCACCC CGCCGGATCG TCTTCTCGGG AAACCTCTGT TTCGAGAGAG TCTCACCTCC 
CACGCATCGT CTCATGAGGA AATGTCGAGT GAGGACTTGG CAATGGCGGA TCCTCAGACG 
AAGATTTTGT ATTTCGCGAA GAGAGATGAG TGGGCGAATG TGGAGTCTGA GATAGAGACT 
ATCAAGCGGA GTGATTTTAG TATGGCTGAT AATCACGGCT TCACCGCCTT CCTCCTAGCC 
GTCAAAGCTG GCAAGGATCA AATCGTAGAC AAGATGATCC GAAAAGGTGC TCGAGTGGAC 
TATAGCACTA AAGATGGCCG TAACGCGACT CATATTGCCG CCATGTACTC CGGAGTTGAA 
ACTCTTGAGC TTATCCTCAA GCGATACTCT GAGCTGCTCC GAAAAGGTGC GGGGCCTAAA 
AAGCAGCTGG CAATCCATGT GGCTTGCGAG AGAAAATCCA AGAAAGCATT TCCAATTGTG 
AAGCGGATTT TGGAAGATAC TGATCAAAGA ATGGCAGAGG ATGGGGATGG ATCCTTGCCG 
ATACACTTGG CATTCAAGTT TGGGAATGTT AATATTGTGG AGCTTCTGCT AAGTGGGCCT 
TCGGATGAAC AAACCAGGAA AGCTGATGGA AACGGGGATA CCTTGCTTCA TTTGGCCGCT 
CGGAGTGGGA ATATCGAAGC GGTTCGGACA GCGATTGCGG CTGGATGTGA TAATGCGAAT 
GTGCAGAATA GGGTGGGAAG GACGCCGCTA CATGAGGTAG CCGAAGTCGG AGATCAAAAT 
ATGCTAAAAA TCATGTTCAA ACTCCGCGCC GACGCCAACA TCCATGATAA GGAGGACAAG 
ACTCCGGTAC ACGTTGCAGC GGAGCGAGGT GACACTTCGA TGGTCGAGTC ACTAATTGAC 
AAGTTTGGTG GCTCAATTCG CGCTAGGACC CGTGATGGGT CGACGCTTCT GCATATTGCC 
GCATGTTCAG GACATACTAG CACCGCATTG GCGTTTTTGA AGAGAGGAGT CCCCCTCTTC 
ATGCCCAACA AAAAAGGAGC CCTGGGTCTT CACTCCGCAG CAGCTGCTGG CTTCAACGAC 
GTCGTCAAAA TGCTCATTGC TCGGGGTACT AATGTAGATG TCCGTACACG AGACAACTAC 
ACCGCTCTCC ACGTAGCGGT TCAATCTGGC AAGGCTTCGG TTGTAGAGAC CCTGCTGGGA 
AGTGGTGCAG ACATTCATGT GAAGGGCGGG GAACTAGGAC AAACTGCACT GCACATTGCG 
GCAAGCTTGA ATGGAGCCGA GAGTCGGGAT TGTGCGATGA TGTTGCTGAA AAGTGGAGGG 
CAGCCGGATG TTGCACAAAT GGATGGGGAG ACTTGTCTGC ATATTGCTGC CAGGAGTGGG 
AATAAGGATA TCATGAGGCT CCTGCTTGAC GAGAACGCCG ACTCGAAAAT AAGCTCAAAG 
ATCGGAGAGA CACCCCTCCA GGTGGCCGCC AAGTCATGCA ATTTTGAAGC AGCATCAATG 
ATTTTGAAGC ACCTTTCGGA AGTTCTGACC CAAGAACAGC TTAAGGAACA TGTCAATCAT 
AGAACCAATG ACGGCTTCAC AGCTCTTCAC TACGCCGCTG AAATCGAGCA GCGCCAGTTA 
CACTTTCCAG GAGAAGATGC CAAGCTAGTA AATCTTCTGA TCGACTACGG TGGAATGGTA 
GAAATGCCAT CACTCAATGC AAATGAGACG GCGATGCATA TGGCGGCAAG ATCCGGAAAT 
CAAGCTGTAC TCCTGGCGAT GGTCAATAAG ATCGGAGCCG GTGCGGTGCA AATCGTGCAG 
AACAAGCAGA GCAAGAACGG ATGGTCACCG CTGTTGGAAG CATGTGCCAG AGGGCATTCT 
GGAGTGGCGA ATATTTTGTT GAAGCACCAC GCCCGTATTG ATGTATTCGA TGAAATGGGC 
CGTACTGCTC TGCACCTGGC AGCTTTCAAT GGGCATCTCT CCCTGGTTCA CCTTCTTCTG 
CAGCACAAAG CATTCGTGAA CAGTAAATCG AAAACCGGAG AGGCACCGCT CCACTTAGCA 
GCTCAGCATG GTCATGTGAA GGTGGTGAAT GTCCTGGTGC AGGATCATGG TGCAGCGCTG 
GAGGCAATTA CGCTGGATAA TCAGACAGCC CTCCACTTTG CCGCAAAATT CGGTCAGCTA 
GCTGTGAGTC AAACCCTTCT GGCTCTCGGA GCAAACCCCA ATGCACGTGA CGACAAGGGT 
CAAACCCCTC TCCATCTGGC AGCTGAGAAT GACTTCCCCG ACGTTGTGAA GCTCTTCCTG 
AAAATGAGAA ATAACAACCG GAGTGTGTTG ACCGCAATTG ATCATAATGG ATTCACCTGC 
GCACATATTG CTGCGATGAA GGGTTCCCTA GCCGTGGTCC GTGAGCTTAT GATGATCGAC 
AAGCCTATGG TAATCCAGGC AAAGACCAAA ACACTGGAAG CCACTACACT TCATATGGCA 
GCTGCGGGAG GTCACGCGAA CATTGTGAAG ATTCTGCTGG AGAATGGAGC AAACGCGGAA 
GATGAGAATT CGCACGGAAT GACTGCTCTC CACCTTGGCG CCAAAAACGG ATTCATATCG 
ATTTTGGAGG CATTCGATAA GATCCTATGG AAACGGTGTT CGAGAAAGAC CGGTCTCAAC 
GCTCTCCACA TCGCTGCGTT CTACGGAAAT TCGGATTTCG TCAATGAAAT GCTCAAGCAC 
GTACAAGCAA CAGTCCGTTC CGAGCCGCCC ATCTACAATC ACCATGTCAA TAAGGAATTC 
TCAACTGAAT ACGGCTTCAC ACCTCTCCAT TTAGCCGCTC AAAGTGGACA CGACAGTCTT 
GTGCGGATGC TTCTGAATCA GGGAGTGCAA GTTGACGCGA CCAGTACTAC AATGAACGTG 
ATCCCCCTCC ATCTGGCTGC CCAGCAAGGC CACATCGCAG TGGTAGGAAT GCTCCTGTCC 
AGATCTACTC AGCAGCAGCA CGCCAAGGAT TGGAGAGGCA GGACCCCGCT CCACCTAGCC 
GCTCAGAATG GCCACTACGA GATGGTCTCA CTTCTCATTG CTCAGGGATC TAACATCAAT 
GTCATGGATC AGAATGGCTG GACTGGTCTT CACTTTGCCA CTCGTGCCGG GCACCTGAGT 
GTCGTCAAGC TGTTCATCGA TAGTTCAGCG GATCCATTGG CGGAGACCAA GGAGGGCAAA 
GTTCCATTGT GCTTTGCTGC AGCTCATAAT CATATAGAAT GTCTTCGATT CCTCCTGAAA 
CAGAAGCATG ACACACATCA ATTGATGGAA GATCGGAAGT TCATATTCGA CTTGATGGTT 
TGTGGTAAAA CCAATGACAA TGAGCCTCTA CAAGAGTTTA TTCTTCAATC ACCTGCTCCA 
ATTGAGACGG CAGTCAAGTT GTCCGCGTTG TACAGAGATA TGTCGGAGAA GGAGAAGGAG 
AGGGCGAAGG ATCTGTTGAA TGTGGCAGTG TTCAGTGAGA ATATGGCTGT GGAGTTGTTA 
GGGATCACCG CCACCGAATA CAATGCCGCT CTTCTCCTGA AGGCTAAGGA CAATCGAGGC 
CGGCCCCTAC TAGATGTTCT CATTGAAAAT GAGCAGAAAG AAGTAGTCTC CTACGCGTCT 
GTCCAACGCT ACCTGACAGA AGTATGGACT GCCCGTGTCG ACTGGTCATT CGGAAAGTTT 
GTCGCATTCT CCCTCTTCGT GCTAATATGC CCCCCGGCAT GGTTCTACTT CTCACTTCCA 
CTGGATAGTC GGATCGGAAG AGCTCCGATT ATTAAATTTG TGTGCCATAT CGTGTCTCAT 
GTCTATTTTA CGATACTGCT GACAATTGTG GTGTTGAATA TTACACATAA GATGTACGAA 
GTAACTTCGG TGGTTCCAAA CCCTGTGGAA TGGCTCCTGT TGCTCTGGCT CTCTGGAAAT 
CTGGTCTCCG AACTCTCCAC TGTCGGTGGA GGATCTGGCC TAGGAATCGT AAAGGTCCTA 
ATCCTAGTCC TTTCCGCGAT GGCGATAGCC GTCCATGTCC TAGCCTTCCT GCTCCCGGCA 
GTATTCCTAA CCCACCTGGA TAACGATGAA AAGCTACATT TCGCCCGGAC AATGCTTTAT 
TTGAAAAATC AACTTTTCGC CTTTGCCCTG CTATTTGCTT TTGTAGAGTA CCTGGATTTC 
CTGACAGTGC ATCATTTGTT CGGTCCCTGG GCGATCATTA TTCGAGATCT AATGTATGAT 
TTGGCCCGTT TCCTTGTGAT CCTGATGTTG TTCGTGGCGG GCTTCACACT CCACGTGACG 
AGTATCTTCC AGCCTGCCTA CCAGCCTGTC GACGAGGACA GCGCCGAGCT GATGCGTCTG 
GCCTCCCCGT CTCAAACCCT CGAAATGCTC TTCTTCTCGC TCTTCGGACT CGTCGAGCCC 
GATTCAATGC CCCCGCTCCA TCTAGTTCCA GATTTTGCAA AAATCATCTT AAAACTTCTA 
TTCGGAATCT ACATGATGGT CACCTTGATT GTGCTGATCA ACTTGCTGAT TGCTATGATG 
TCTGACACCT ACCAACGAAT TCAGGCACAG TCGGATAAGG AATGGAAGTT CGGAAGAGCT 
ATTCTGATCA GACAGATGAA TAAGAAAAGC GCCACGCCGT CGCCGATAAA TATGTTAACA 
AAGTTGATAA TTGTGCTGAG GGTAGCCTGG CGGAATCGGT TGAGATGCAT GACCCGAAAA 
GCCCAAGACG ATCTTCGCTT CGAGGAGAAC ATCGACGCGT TCTCCATGGG TGGCGGCCAG 
CAGGGAAGGC AAAGTCCGAC CAATGAAGGA AGAGAAGGCC AGCAAGAGCT TGGTAACTCG 
GCTGACTGGA ACATCGAGAC AGTCATCGAC TGGAGGAAGA TTGTTTCAAT GTACTATCAG 
GCGAATGGGA AGCTTACAGA CGGGCGAACC AAAGAGGATG TGGATTTGGC AATGGCAGTA 
CCTACTAGTT TTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trp-4 ( NM_061028.3 ) cDNA ORF clone, Caenorhabditis elegans -> NP_493429.2 Caenorhabditis elegans ANK_REP_REGION domain-containing protein (trp-4), mRNA.

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CloneID	OCa335587
Clone ID Related Accession (Same CDS sequence)	NM_061028.2 , NM_061028.3
Accession Version	NM_061028.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5775bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	ANK_REP_REGION domain-containing protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003279). On Sep 4, 2019 this sequence version replaced NM_061028.2.

ATGGATTCGC CACGTGGCGG AATCCTGGGA AGAGCTTTAC GAGAAGCATC ACAATCGACT 
AGGCAAGAAA ATGATGTTGA TATGGATCAG GTACCCGTAC GGCAGATGAA CAGGGATTAC 
GGTGGATCCA GGAGGACTCA GATGAATCCC CACACCTCCC AACCTGGTCC ATCTCATGTA 
TCAATTGTAA ATGTCCCAGA ACGCGGAGGA CCCACATCTT CCACATCAAC CACACATGAG 
ACAGAGCACA CGGCACATAG GACAGAGTCC GGGAGGTTTA TCAGACGCCG TCGCCAATCT 
CGAGAGGTTA CCACCACAAC CACAAGACCC TATGACCCCG CTCCTCCAAC CCAGACCCGA 
ACAAGCTCCG GCTCAACAGT AAATGGATGG GGGGAGAATC GACCGAAATC TGCTGATGAG 
GAGATCAAAC GGCGGCGAAG AAGTGGCGGG GGAATCCTGT CTCGCGGGCT TCGAGAAATG 
AACAAAATGG TGGAAGAGTT GGAGCAAGCA AGTGAAGAGC CAAGTACCAG GAAGGGAATT 
CTGGGTACTG CGTTAAAGGA TATGGAAGGG ACCACTTATC AAAAGATTTA CAGGAAAAGG 
GAGGAAACTC CCAAGCGCTC CCGTTCATTT GACGATCAGG AGATGTCGAA TCGAGTAGGA 
ATGATCGAGC ACTTGCTCCG AGACAAGGAT CCTTTGGAGC TTCAGCAGTT GGGATTAACC 
GACCTCCTCA CCACCGACAC CATCCCAACT GACCGACCAC CCCTCCGCCG ATCCTCGACC 
CATCTCCAAA TCGGAAAGAA CTCACGGATC ATCTTCGTTC CGAAACAACC ATCCCGTGAT 
TCAGTCACCC CGCCGGATCG TCTTCTCGGG AAACCTCTGT TTCGAGAGAG TCTCACCTCC 
CACGCATCGT CTCATGAGGA AATGTCGAGT GAGGACTTGG CAATGGCGGA TCCTCAGACG 
AAGATTTTGT ATTTCGCGAA GAGAGATGAG TGGGCGAATG TGGAGTCTGA GATAGAGACT 
ATCAAGCGGA GTGATTTTAG TATGGCTGAT AATCACGGCT TCACCGCCTT CCTCCTAGCC 
GTCAAAGCTG GCAAGGATCA AATCGTAGAC AAGATGATCC GAAAAGGTGC TCGAGTGGAC 
TATAGCACTA AAGATGGCCG TAACGCGACT CATATTGCCG CCATGTACTC CGGAGTTGAA 
ACTCTTGAGC TTATCCTCAA GCGATACTCT GAGCTGCTCC GAAAAGGTGC GGGGCCTAAA 
AAGCAGCTGG CAATCCATGT GGCTTGCGAG AGAAAATCCA AGAAAGCATT TCCAATTGTG 
AAGCGGATTT TGGAAGATAC TGATCAAAGA ATGGCAGAGG ATGGGGATGG ATCCTTGCCG 
ATACACTTGG CATTCAAGTT TGGGAATGTT AATATTGTGG AGCTTCTGCT AAGTGGGCCT 
TCGGATGAAC AAACCAGGAA AGCTGATGGA AACGGGGATA CCTTGCTTCA TTTGGCCGCT 
CGGAGTGGGA ATATCGAAGC GGTTCGGACA GCGATTGCGG CTGGATGTGA TAATGCGAAT 
GTGCAGAATA GGGTGGGAAG GACGCCGCTA CATGAGGTAG CCGAAGTCGG AGATCAAAAT 
ATGCTAAAAA TCATGTTCAA ACTCCGCGCC GACGCCAACA TCCATGATAA GGAGGACAAG 
ACTCCGGTAC ACGTTGCAGC GGAGCGAGGT GACACTTCGA TGGTCGAGTC ACTAATTGAC 
AAGTTTGGTG GCTCAATTCG CGCTAGGACC CGTGATGGGT CGACGCTTCT GCATATTGCC 
GCATGTTCAG GACATACTAG CACCGCATTG GCGTTTTTGA AGAGAGGAGT CCCCCTCTTC 
ATGCCCAACA AAAAAGGAGC CCTGGGTCTT CACTCCGCAG CAGCTGCTGG CTTCAACGAC 
GTCGTCAAAA TGCTCATTGC TCGGGGTACT AATGTAGATG TCCGTACACG AGACAACTAC 
ACCGCTCTCC ACGTAGCGGT TCAATCTGGC AAGGCTTCGG TTGTAGAGAC CCTGCTGGGA 
AGTGGTGCAG ACATTCATGT GAAGGGCGGG GAACTAGGAC AAACTGCACT GCACATTGCG 
GCAAGCTTGA ATGGAGCCGA GAGTCGGGAT TGTGCGATGA TGTTGCTGAA AAGTGGAGGG 
CAGCCGGATG TTGCACAAAT GGATGGGGAG ACTTGTCTGC ATATTGCTGC CAGGAGTGGG 
AATAAGGATA TCATGAGGCT CCTGCTTGAC GAGAACGCCG ACTCGAAAAT AAGCTCAAAG 
ATCGGAGAGA CACCCCTCCA GGTGGCCGCC AAGTCATGCA ATTTTGAAGC AGCATCAATG 
ATTTTGAAGC ACCTTTCGGA AGTTCTGACC CAAGAACAGC TTAAGGAACA TGTCAATCAT 
AGAACCAATG ACGGCTTCAC AGCTCTTCAC TACGCCGCTG AAATCGAGCA GCGCCAGTTA 
CACTTTCCAG GAGAAGATGC CAAGCTAGTA AATCTTCTGA TCGACTACGG TGGAATGGTA 
GAAATGCCAT CACTCAATGC AAATGAGACG GCGATGCATA TGGCGGCAAG ATCCGGAAAT 
CAAGCTGTAC TCCTGGCGAT GGTCAATAAG ATCGGAGCCG GTGCGGTGCA AATCGTGCAG 
AACAAGCAGA GCAAGAACGG ATGGTCACCG CTGTTGGAAG CATGTGCCAG AGGGCATTCT 
GGAGTGGCGA ATATTTTGTT GAAGCACCAC GCCCGTATTG ATGTATTCGA TGAAATGGGC 
CGTACTGCTC TGCACCTGGC AGCTTTCAAT GGGCATCTCT CCCTGGTTCA CCTTCTTCTG 
CAGCACAAAG CATTCGTGAA CAGTAAATCG AAAACCGGAG AGGCACCGCT CCACTTAGCA 
GCTCAGCATG GTCATGTGAA GGTGGTGAAT GTCCTGGTGC AGGATCATGG TGCAGCGCTG 
GAGGCAATTA CGCTGGATAA TCAGACAGCC CTCCACTTTG CCGCAAAATT CGGTCAGCTA 
GCTGTGAGTC AAACCCTTCT GGCTCTCGGA GCAAACCCCA ATGCACGTGA CGACAAGGGT 
CAAACCCCTC TCCATCTGGC AGCTGAGAAT GACTTCCCCG ACGTTGTGAA GCTCTTCCTG 
AAAATGAGAA ATAACAACCG GAGTGTGTTG ACCGCAATTG ATCATAATGG ATTCACCTGC 
GCACATATTG CTGCGATGAA GGGTTCCCTA GCCGTGGTCC GTGAGCTTAT GATGATCGAC 
AAGCCTATGG TAATCCAGGC AAAGACCAAA ACACTGGAAG CCACTACACT TCATATGGCA 
GCTGCGGGAG GTCACGCGAA CATTGTGAAG ATTCTGCTGG AGAATGGAGC AAACGCGGAA 
GATGAGAATT CGCACGGAAT GACTGCTCTC CACCTTGGCG CCAAAAACGG ATTCATATCG 
ATTTTGGAGG CATTCGATAA GATCCTATGG AAACGGTGTT CGAGAAAGAC CGGTCTCAAC 
GCTCTCCACA TCGCTGCGTT CTACGGAAAT TCGGATTTCG TCAATGAAAT GCTCAAGCAC 
GTACAAGCAA CAGTCCGTTC CGAGCCGCCC ATCTACAATC ACCATGTCAA TAAGGAATTC 
TCAACTGAAT ACGGCTTCAC ACCTCTCCAT TTAGCCGCTC AAAGTGGACA CGACAGTCTT 
GTGCGGATGC TTCTGAATCA GGGAGTGCAA GTTGACGCGA CCAGTACTAC AATGAACGTG 
ATCCCCCTCC ATCTGGCTGC CCAGCAAGGC CACATCGCAG TGGTAGGAAT GCTCCTGTCC 
AGATCTACTC AGCAGCAGCA CGCCAAGGAT TGGAGAGGCA GGACCCCGCT CCACCTAGCC 
GCTCAGAATG GCCACTACGA GATGGTCTCA CTTCTCATTG CTCAGGGATC TAACATCAAT 
GTCATGGATC AGAATGGCTG GACTGGTCTT CACTTTGCCA CTCGTGCCGG GCACCTGAGT 
GTCGTCAAGC TGTTCATCGA TAGTTCAGCG GATCCATTGG CGGAGACCAA GGAGGGCAAA 
GTTCCATTGT GCTTTGCTGC AGCTCATAAT CATATAGAAT GTCTTCGATT CCTCCTGAAA 
CAGAAGCATG ACACACATCA ATTGATGGAA GATCGGAAGT TCATATTCGA CTTGATGGTT 
TGTGGTAAAA CCAATGACAA TGAGCCTCTA CAAGAGTTTA TTCTTCAATC ACCTGCTCCA 
ATTGAGACGG CAGTCAAGTT GTCCGCGTTG TACAGAGATA TGTCGGAGAA GGAGAAGGAG 
AGGGCGAAGG ATCTGTTGAA TGTGGCAGTG TTCAGTGAGA ATATGGCTGT GGAGTTGTTA 
GGGATCACCG CCACCGAATA CAATGCCGCT CTTCTCCTGA AGGCTAAGGA CAATCGAGGC 
CGGCCCCTAC TAGATGTTCT CATTGAAAAT GAGCAGAAAG AAGTAGTCTC CTACGCGTCT 
GTCCAACGCT ACCTGACAGA AGTATGGACT GCCCGTGTCG ACTGGTCATT CGGAAAGTTT 
GTCGCATTCT CCCTCTTCGT GCTAATATGC CCCCCGGCAT GGTTCTACTT CTCACTTCCA 
CTGGATAGTC GGATCGGAAG AGCTCCGATT ATTAAATTTG TGTGCCATAT CGTGTCTCAT 
GTCTATTTTA CGATACTGCT GACAATTGTG GTGTTGAATA TTACACATAA GATGTACGAA 
GTAACTTCGG TGGTTCCAAA CCCTGTGGAA TGGCTCCTGT TGCTCTGGCT CTCTGGAAAT 
CTGGTCTCCG AACTCTCCAC TGTCGGTGGA GGATCTGGCC TAGGAATCGT AAAGGTCCTA 
ATCCTAGTCC TTTCCGCGAT GGCGATAGCC GTCCATGTCC TAGCCTTCCT GCTCCCGGCA 
GTATTCCTAA CCCACCTGGA TAACGATGAA AAGCTACATT TCGCCCGGAC AATGCTTTAT 
TTGAAAAATC AACTTTTCGC CTTTGCCCTG CTATTTGCTT TTGTAGAGTA CCTGGATTTC 
CTGACAGTGC ATCATTTGTT CGGTCCCTGG GCGATCATTA TTCGAGATCT AATGTATGAT 
TTGGCCCGTT TCCTTGTGAT CCTGATGTTG TTCGTGGCGG GCTTCACACT CCACGTGACG 
AGTATCTTCC AGCCTGCCTA CCAGCCTGTC GACGAGGACA GCGCCGAGCT GATGCGTCTG 
GCCTCCCCGT CTCAAACCCT CGAAATGCTC TTCTTCTCGC TCTTCGGACT CGTCGAGCCC 
GATTCAATGC CCCCGCTCCA TCTAGTTCCA GATTTTGCAA AAATCATCTT AAAACTTCTA 
TTCGGAATCT ACATGATGGT CACCTTGATT GTGCTGATCA ACTTGCTGAT TGCTATGATG 
TCTGACACCT ACCAACGAAT TCAGGCACAG TCGGATAAGG AATGGAAGTT CGGAAGAGCT 
ATTCTGATCA GACAGATGAA TAAGAAAAGC GCCACGCCGT CGCCGATAAA TATGTTAACA 
AAGTTGATAA TTGTGCTGAG GGTAGCCTGG CGGAATCGGT TGAGATGCAT GACCCGAAAA 
GCCCAAGACG ATCTTCGCTT CGAGGAGAAC ATCGACGCGT TCTCCATGGG TGGCGGCCAG 
CAGGGAAGGC AAAGTCCGAC CAATGAAGGA AGAGAAGGCC AGCAAGAGCT TGGTAACTCG 
GCTGACTGGA ACATCGAGAC AGTCATCGAC TGGAGGAAGA TTGTTTCAAT GTACTATCAG 
GCGAATGGGA AGCTTACAGA CGGGCGAACC AAAGAGGATG TGGATTTGGC AATGGCAGTA 
CCTACTAGTT TTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_493429.2
CDS	85..5859
Translation	MDSPRGGILGRALREASQSTRQENDVDMDQVPVRQMNRDYGGSRRTQMNPHTSQPGPSHVSIVNVPERGGPTSSTSTTHETEHTAHRTESGRFIRRRRQSREVTTTTTRPYDPAPPTQTRTSSGSTVNGWGENRPKSADEEIKRRRRSGGGILSRGLREMNKMVEELEQASEEPSTRKGILGTALKDMEGTTYQKIYRKREETPKRSRSFDDQEMSNRVGMIEHLLRDKDPLELQQLGLTDLLTTDTIPTDRPPLRRSSTHLQIGKNSRIIFVPKQPSRDSVTPPDRLLGKPLFRESLTSHASSHEEMSSEDLAMADPQTKILYFAKRDEWANVESEIETIKRSDFSMADNHGFTAFLLAVKAGKDQIVDKMIRKGARVDYSTKDGRNATHIAAMYSGVETLELILKRYSELLRKGAGPKKQLAIHVACERKSKKAFPIVKRILEDTDQRMAEDGDGSLPIHLAFKFGNVNIVELLLSGPSDEQTRKADGNGDTLLHLAARSGNIEAVRTAIAAGCDNANVQNRVGRTPLHEVAEVGDQNMLKIMFKLRADANIHDKEDKTPVHVAAERGDTSMVESLIDKFGGSIRARTRDGSTLLHIAACSGHTSTALAFLKRGVPLFMPNKKGALGLHSAAAAGFNDVVKMLIARGTNVDVRTRDNYTALHVAVQSGKASVVETLLGSGADIHVKGGELGQTALHIAASLNGAESRDCAMMLLKSGGQPDVAQMDGETCLHIAARSGNKDIMRLLLDENADSKISSKIGETPLQVAAKSCNFEAASMILKHLSEVLTQEQLKEHVNHRTNDGFTALHYAAEIEQRQLHFPGEDAKLVNLLIDYGGMVEMPSLNANETAMHMAARSGNQAVLLAMVNKIGAGAVQIVQNKQSKNGWSPLLEACARGHSGVANILLKHHARIDVFDEMGRTALHLAAFNGHLSLVHLLLQHKAFVNSKSKTGEAPLHLAAQHGHVKVVNVLVQDHGAALEAITLDNQTALHFAAKFGQLAVSQTLLALGANPNARDDKGQTPLHLAAENDFPDVVKLFLKMRNNNRSVLTAIDHNGFTCAHIAAMKGSLAVVRELMMIDKPMVIQAKTKTLEATTLHMAAAGGHANIVKILLENGANAEDENSHGMTALHLGAKNGFISILEAFDKILWKRCSRKTGLNALHIAAFYGNSDFVNEMLKHVQATVRSEPPIYNHHVNKEFSTEYGFTPLHLAAQSGHDSLVRMLLNQGVQVDATSTTMNVIPLHLAAQQGHIAVVGMLLSRSTQQQHAKDWRGRTPLHLAAQNGHYEMVSLLIAQGSNINVMDQNGWTGLHFATRAGHLSVVKLFIDSSADPLAETKEGKVPLCFAAAHNHIECLRFLLKQKHDTHQLMEDRKFIFDLMVCGKTNDNEPLQEFILQSPAPIETAVKLSALYRDMSEKEKERAKDLLNVAVFSENMAVELLGITATEYNAALLLKAKDNRGRPLLDVLIENEQKEVVSYASVQRYLTEVWTARVDWSFGKFVAFSLFVLICPPAWFYFSLPLDSRIGRAPIIKFVCHIVSHVYFTILLTIVVLNITHKMYEVTSVVPNPVEWLLLLWLSGNLVSELSTVGGGSGLGIVKVLILVLSAMAIAVHVLAFLLPAVFLTHLDNDEKLHFARTMLYLKNQLFAFALLFAFVEYLDFLTVHHLFGPWAIIIRDLMYDLARFLVILMLFVAGFTLHVTSIFQPAYQPVDEDSAELMRLASPSQTLEMLFFSLFGLVEPDSMPPLHLVPDFAKIILKLLFGIYMMVTLIVLINLLIAMMSDTYQRIQAQSDKEWKFGRAILIRQMNKKSATPSPINMLTKLIIVLRVAWRNRLRCMTRKAQDDLRFEENIDAFSMGGGQQGRQSPTNEGREGQQELGNSADWNIETVIDWRKIVSMYYQANGKLTDGRTKEDVDLAMAVPTSF

Target ORF information:

RefSeq Version	NM_061028.3
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans ANK_REP_REGION domain-containing protein (trp-4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_061028.3

ORF Insert Sequence:

ATGGATTCGC CACGTGGCGG AATCCTGGGA AGAGCTTTAC GAGAAGCATC ACAATCGACT 
AGGCAAGAAA ATGATGTTGA TATGGATCAG GTACCCGTAC GGCAGATGAA CAGGGATTAC 
GGTGGATCCA GGAGGACTCA GATGAATCCC CACACCTCCC AACCTGGTCC ATCTCATGTA 
TCAATTGTAA ATGTCCCAGA ACGCGGAGGA CCCACATCTT CCACATCAAC CACACATGAG 
ACAGAGCACA CGGCACATAG GACAGAGTCC GGGAGGTTTA TCAGACGCCG TCGCCAATCT 
CGAGAGGTTA CCACCACAAC CACAAGACCC TATGACCCCG CTCCTCCAAC CCAGACCCGA 
ACAAGCTCCG GCTCAACAGT AAATGGATGG GGGGAGAATC GACCGAAATC TGCTGATGAG 
GAGATCAAAC GGCGGCGAAG AAGTGGCGGG GGAATCCTGT CTCGCGGGCT TCGAGAAATG 
AACAAAATGG TGGAAGAGTT GGAGCAAGCA AGTGAAGAGC CAAGTACCAG GAAGGGAATT 
CTGGGTACTG CGTTAAAGGA TATGGAAGGG ACCACTTATC AAAAGATTTA CAGGAAAAGG 
GAGGAAACTC CCAAGCGCTC CCGTTCATTT GACGATCAGG AGATGTCGAA TCGAGTAGGA 
ATGATCGAGC ACTTGCTCCG AGACAAGGAT CCTTTGGAGC TTCAGCAGTT GGGATTAACC 
GACCTCCTCA CCACCGACAC CATCCCAACT GACCGACCAC CCCTCCGCCG ATCCTCGACC 
CATCTCCAAA TCGGAAAGAA CTCACGGATC ATCTTCGTTC CGAAACAACC ATCCCGTGAT 
TCAGTCACCC CGCCGGATCG TCTTCTCGGG AAACCTCTGT TTCGAGAGAG TCTCACCTCC 
CACGCATCGT CTCATGAGGA AATGTCGAGT GAGGACTTGG CAATGGCGGA TCCTCAGACG 
AAGATTTTGT ATTTCGCGAA GAGAGATGAG TGGGCGAATG TGGAGTCTGA GATAGAGACT 
ATCAAGCGGA GTGATTTTAG TATGGCTGAT AATCACGGCT TCACCGCCTT CCTCCTAGCC 
GTCAAAGCTG GCAAGGATCA AATCGTAGAC AAGATGATCC GAAAAGGTGC TCGAGTGGAC 
TATAGCACTA AAGATGGCCG TAACGCGACT CATATTGCCG CCATGTACTC CGGAGTTGAA 
ACTCTTGAGC TTATCCTCAA GCGATACTCT GAGCTGCTCC GAAAAGGTGC GGGGCCTAAA 
AAGCAGCTGG CAATCCATGT GGCTTGCGAG AGAAAATCCA AGAAAGCATT TCCAATTGTG 
AAGCGGATTT TGGAAGATAC TGATCAAAGA ATGGCAGAGG ATGGGGATGG ATCCTTGCCG 
ATACACTTGG CATTCAAGTT TGGGAATGTT AATATTGTGG AGCTTCTGCT AAGTGGGCCT 
TCGGATGAAC AAACCAGGAA AGCTGATGGA AACGGGGATA CCTTGCTTCA TTTGGCCGCT 
CGGAGTGGGA ATATCGAAGC GGTTCGGACA GCGATTGCGG CTGGATGTGA TAATGCGAAT 
GTGCAGAATA GGGTGGGAAG GACGCCGCTA CATGAGGTAG CCGAAGTCGG AGATCAAAAT 
ATGCTAAAAA TCATGTTCAA ACTCCGCGCC GACGCCAACA TCCATGATAA GGAGGACAAG 
ACTCCGGTAC ACGTTGCAGC GGAGCGAGGT GACACTTCGA TGGTCGAGTC ACTAATTGAC 
AAGTTTGGTG GCTCAATTCG CGCTAGGACC CGTGATGGGT CGACGCTTCT GCATATTGCC 
GCATGTTCAG GACATACTAG CACCGCATTG GCGTTTTTGA AGAGAGGAGT CCCCCTCTTC 
ATGCCCAACA AAAAAGGAGC CCTGGGTCTT CACTCCGCAG CAGCTGCTGG CTTCAACGAC 
GTCGTCAAAA TGCTCATTGC TCGGGGTACT AATGTAGATG TCCGTACACG AGACAACTAC 
ACCGCTCTCC ACGTAGCGGT TCAATCTGGC AAGGCTTCGG TTGTAGAGAC CCTGCTGGGA 
AGTGGTGCAG ACATTCATGT GAAGGGCGGG GAACTAGGAC AAACTGCACT GCACATTGCG 
GCAAGCTTGA ATGGAGCCGA GAGTCGGGAT TGTGCGATGA TGTTGCTGAA AAGTGGAGGG 
CAGCCGGATG TTGCACAAAT GGATGGGGAG ACTTGTCTGC ATATTGCTGC CAGGAGTGGG 
AATAAGGATA TCATGAGGCT CCTGCTTGAC GAGAACGCCG ACTCGAAAAT AAGCTCAAAG 
ATCGGAGAGA CACCCCTCCA GGTGGCCGCC AAGTCATGCA ATTTTGAAGC AGCATCAATG 
ATTTTGAAGC ACCTTTCGGA AGTTCTGACC CAAGAACAGC TTAAGGAACA TGTCAATCAT 
AGAACCAATG ACGGCTTCAC AGCTCTTCAC TACGCCGCTG AAATCGAGCA GCGCCAGTTA 
CACTTTCCAG GAGAAGATGC CAAGCTAGTA AATCTTCTGA TCGACTACGG TGGAATGGTA 
GAAATGCCAT CACTCAATGC AAATGAGACG GCGATGCATA TGGCGGCAAG ATCCGGAAAT 
CAAGCTGTAC TCCTGGCGAT GGTCAATAAG ATCGGAGCCG GTGCGGTGCA AATCGTGCAG 
AACAAGCAGA GCAAGAACGG ATGGTCACCG CTGTTGGAAG CATGTGCCAG AGGGCATTCT 
GGAGTGGCGA ATATTTTGTT GAAGCACCAC GCCCGTATTG ATGTATTCGA TGAAATGGGC 
CGTACTGCTC TGCACCTGGC AGCTTTCAAT GGGCATCTCT CCCTGGTTCA CCTTCTTCTG 
CAGCACAAAG CATTCGTGAA CAGTAAATCG AAAACCGGAG AGGCACCGCT CCACTTAGCA 
GCTCAGCATG GTCATGTGAA GGTGGTGAAT GTCCTGGTGC AGGATCATGG TGCAGCGCTG 
GAGGCAATTA CGCTGGATAA TCAGACAGCC CTCCACTTTG CCGCAAAATT CGGTCAGCTA 
GCTGTGAGTC AAACCCTTCT GGCTCTCGGA GCAAACCCCA ATGCACGTGA CGACAAGGGT 
CAAACCCCTC TCCATCTGGC AGCTGAGAAT GACTTCCCCG ACGTTGTGAA GCTCTTCCTG 
AAAATGAGAA ATAACAACCG GAGTGTGTTG ACCGCAATTG ATCATAATGG ATTCACCTGC 
GCACATATTG CTGCGATGAA GGGTTCCCTA GCCGTGGTCC GTGAGCTTAT GATGATCGAC 
AAGCCTATGG TAATCCAGGC AAAGACCAAA ACACTGGAAG CCACTACACT TCATATGGCA 
GCTGCGGGAG GTCACGCGAA CATTGTGAAG ATTCTGCTGG AGAATGGAGC AAACGCGGAA 
GATGAGAATT CGCACGGAAT GACTGCTCTC CACCTTGGCG CCAAAAACGG ATTCATATCG 
ATTTTGGAGG CATTCGATAA GATCCTATGG AAACGGTGTT CGAGAAAGAC CGGTCTCAAC 
GCTCTCCACA TCGCTGCGTT CTACGGAAAT TCGGATTTCG TCAATGAAAT GCTCAAGCAC 
GTACAAGCAA CAGTCCGTTC CGAGCCGCCC ATCTACAATC ACCATGTCAA TAAGGAATTC 
TCAACTGAAT ACGGCTTCAC ACCTCTCCAT TTAGCCGCTC AAAGTGGACA CGACAGTCTT 
GTGCGGATGC TTCTGAATCA GGGAGTGCAA GTTGACGCGA CCAGTACTAC AATGAACGTG 
ATCCCCCTCC ATCTGGCTGC CCAGCAAGGC CACATCGCAG TGGTAGGAAT GCTCCTGTCC 
AGATCTACTC AGCAGCAGCA CGCCAAGGAT TGGAGAGGCA GGACCCCGCT CCACCTAGCC 
GCTCAGAATG GCCACTACGA GATGGTCTCA CTTCTCATTG CTCAGGGATC TAACATCAAT 
GTCATGGATC AGAATGGCTG GACTGGTCTT CACTTTGCCA CTCGTGCCGG GCACCTGAGT 
GTCGTCAAGC TGTTCATCGA TAGTTCAGCG GATCCATTGG CGGAGACCAA GGAGGGCAAA 
GTTCCATTGT GCTTTGCTGC AGCTCATAAT CATATAGAAT GTCTTCGATT CCTCCTGAAA 
CAGAAGCATG ACACACATCA ATTGATGGAA GATCGGAAGT TCATATTCGA CTTGATGGTT 
TGTGGTAAAA CCAATGACAA TGAGCCTCTA CAAGAGTTTA TTCTTCAATC ACCTGCTCCA 
ATTGAGACGG CAGTCAAGTT GTCCGCGTTG TACAGAGATA TGTCGGAGAA GGAGAAGGAG 
AGGGCGAAGG ATCTGTTGAA TGTGGCAGTG TTCAGTGAGA ATATGGCTGT GGAGTTGTTA 
GGGATCACCG CCACCGAATA CAATGCCGCT CTTCTCCTGA AGGCTAAGGA CAATCGAGGC 
CGGCCCCTAC TAGATGTTCT CATTGAAAAT GAGCAGAAAG AAGTAGTCTC CTACGCGTCT 
GTCCAACGCT ACCTGACAGA AGTATGGACT GCCCGTGTCG ACTGGTCATT CGGAAAGTTT 
GTCGCATTCT CCCTCTTCGT GCTAATATGC CCCCCGGCAT GGTTCTACTT CTCACTTCCA 
CTGGATAGTC GGATCGGAAG AGCTCCGATT ATTAAATTTG TGTGCCATAT CGTGTCTCAT 
GTCTATTTTA CGATACTGCT GACAATTGTG GTGTTGAATA TTACACATAA GATGTACGAA 
GTAACTTCGG TGGTTCCAAA CCCTGTGGAA TGGCTCCTGT TGCTCTGGCT CTCTGGAAAT 
CTGGTCTCCG AACTCTCCAC TGTCGGTGGA GGATCTGGCC TAGGAATCGT AAAGGTCCTA 
ATCCTAGTCC TTTCCGCGAT GGCGATAGCC GTCCATGTCC TAGCCTTCCT GCTCCCGGCA 
GTATTCCTAA CCCACCTGGA TAACGATGAA AAGCTACATT TCGCCCGGAC AATGCTTTAT 
TTGAAAAATC AACTTTTCGC CTTTGCCCTG CTATTTGCTT TTGTAGAGTA CCTGGATTTC 
CTGACAGTGC ATCATTTGTT CGGTCCCTGG GCGATCATTA TTCGAGATCT AATGTATGAT 
TTGGCCCGTT TCCTTGTGAT CCTGATGTTG TTCGTGGCGG GCTTCACACT CCACGTGACG 
AGTATCTTCC AGCCTGCCTA CCAGCCTGTC GACGAGGACA GCGCCGAGCT GATGCGTCTG 
GCCTCCCCGT CTCAAACCCT CGAAATGCTC TTCTTCTCGC TCTTCGGACT CGTCGAGCCC 
GATTCAATGC CCCCGCTCCA TCTAGTTCCA GATTTTGCAA AAATCATCTT AAAACTTCTA 
TTCGGAATCT ACATGATGGT CACCTTGATT GTGCTGATCA ACTTGCTGAT TGCTATGATG 
TCTGACACCT ACCAACGAAT TCAGGCACAG TCGGATAAGG AATGGAAGTT CGGAAGAGCT 
ATTCTGATCA GACAGATGAA TAAGAAAAGC GCCACGCCGT CGCCGATAAA TATGTTAACA 
AAGTTGATAA TTGTGCTGAG GGTAGCCTGG CGGAATCGGT TGAGATGCAT GACCCGAAAA 
GCCCAAGACG ATCTTCGCTT CGAGGAGAAC ATCGACGCGT TCTCCATGGG TGGCGGCCAG 
CAGGGAAGGC AAAGTCCGAC CAATGAAGGA AGAGAAGGCC AGCAAGAGCT TGGTAACTCG 
GCTGACTGGA ACATCGAGAC AGTCATCGAC TGGAGGAAGA TTGTTTCAAT GTACTATCAG 
GCGAATGGGA AGCTTACAGA CGGGCGAACC AAAGAGGATG TGGATTTGGC AATGGCAGTA 
CCTACTAGTT TTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.
Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

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trp-4 cDNA ORF clone, Caenorhabditis elegans

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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