cgh-1 cDNA ORF clone, Caenorhabditis elegans

Gene
Clones
gRNAs

Summary

Gene Symbol	cgh-1
Entrez Gene ID	176061
Full Name	ATP-dependent RNA helicase cgh-1
Gene Type	protein-coding
Organism	Caenorhabditis elegans

mRNA and Protein(s)

mRNA	Protein	Name
NM_066245.6	NP_498646.1	ATP-dependent RNA helicase cgh-1
NM_066245.5	NP_498646.1	ATP-dependent RNA helicase cgh-1

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009135	XP_319893.3
Magnaporthe oryzae (rice blast fungus)	MGG_03388	XP_003716556.1
Neurospora crassa	NCU06149	XP_960011.2
Pan troglodytes (chimpanzee)	DDX6	XP_508798.2
Mus musculus (house mouse)	Ddx6	NP_851841.2
Gallus gallus (chicken)	DDX6	NP_001006319.2
Saccharomyces cerevisiae (baker's yeast)	DHH1	NP_010121.1
Schizosaccharomyces pombe (fission yeast)	ste13	NP_596324.1
Homo sapiens (human)	DDX6	NP_001244120.1
Canis lupus familiaris (dog)	DDX6	XP_005619753.1
Danio rerio (zebrafish)	ddx6	XP_684923.1
Eremothecium gossypii	AGOS_ACR197W	NP_983599.1
Oryza sativa (rice)	Os04g0533000	NP_001053408.1
Oryza sativa (rice)	Os10g0503700	NP_001065000.2
Xenopus tropicalis (tropical clawed frog)	ddx6	NP_001072584.1
Caenorhabditis elegans (roundworm)	cgh-1	NP_498646.1
Arabidopsis thaliana (thale cress)	RH8	NP_849535.1
Oryza sativa (rice)	Os02g0641800	NP_001047552.1
Bos taurus (cattle)	DDX6	NP_001137339.1
Rattus norvegicus (Norway rat)	Ddx6	NP_001102762.1
Drosophila melanogaster (fruit fly)	me31B	NP_523533.2
Kluyveromyces lactis	KLLA0C16599g	XP_452942.1

Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.

Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data show that DEAD-box RNA helicase, CGH-1/DDX6, as a key casein kinase II (CK2) substrate and phosphorylation of a conserved N-terminal serine is required for CGH-1 function in the microRNA (miRNA) pathway.
Title: Casein kinase II promotes target silencing by miRISC through direct phosphorylation of the DEAD-box RNA helicase CGH-1.

These results identify somatic and germ cell CGH-1 functions that are distinguished by the involvement of PATR-1, and reveal that during oogenesis, numerous translationally regulated mRNAs are specifically protected by a CGH-1-dependent mechanism.
Title: Protection of specific maternal messenger RNAs by the P body protein CGH-1 (Dhh1/RCK) during Caenorhabditis elegans oogenesis.

Regulation of physiological germ cell apoptosis is specifically influenced by certain functions of the CGH-1/CAR-1 RNA-proteinP complex.
Title: A conserved RNA-protein complex component involved in physiological germline apoptosis regulation in C. elegans.

in developing oocytes CGH-1 particles accumulate specifically in response to meiotic development
Title: Requirement for P granules and meiosis for accumulation of the germline RNA helicase CGH-1.

The following cgh-1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the cgh-1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa340986	NM_066245.6 Latest version!	Caenorhabditis elegans ATP-dependent RNA helicase cgh-1 (cgh-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
View Old Accession Versions >>
OCa340986	NM_066245.5	Caenorhabditis elegans ATP-dependent RNA helicase cgh-1 (cgh-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

cgh-1 ( NM_066245.6 ) cDNA ORF clone, Caenorhabditis elegans -> NP_498646.1 Caenorhabditis elegans ATP-dependent RNA helicase cgh-1 (cgh-1), mRNA.

Price & Availability↑

CloneID	OCa340986
Clone ID Related Accession (Same CDS sequence)	NM_066245.6 , NM_066245.5
Accession Version	NM_066245.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1293bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	ATP-dependent RNA helicase cgh-1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003281). On Sep 4, 2019 this sequence version replaced NM_066245.5.

ATGAGTGGAG CGGAGCAACA ACAGATCGTT CCTGCCAACA ATGGCGACGA AAACTGGAAG 
GCTGGTCTCA ATCTTCCAGC AAAAGACAGA AGATTCAAAA CTGCTGATGT CACTGATACG 
AAGGGAGTCG AGTTTGAGGA TTTCTGCCTT GGACGCGATC TTCTCATGGG GATTTTTGAG 
AAAGGATGGG AAAAACCGAG TCCTATTCAA GAAGCATCCA TCGGAGTCGC TCTCACTGGT 
CAAGATATTC TCGCAAGAGC TAAGAACGGA ACAGGAAAAA CCGGAGCTTA TTGTATCCCT 
GTTATCGAGA AGATCCAGCC AGCCCTCAAA GCTATTCAAG CCATGGTCAT TGTCCCAACT 
CGTGAATTGG CTCTCCAAAC TTCACAGATC TGCGTGGAGC TCTCCAAACA CATTCAGCTC 
AAAGTTATGG TCACTACCGG AGGAACTGAT CTCCGTGATG ATATCATGCG TCTAAACGGA 
ACGGTTCACT TGGTCATCGC CACCCCAGGA AGAATTCTCG ATCTTATGGA GAAAGGAGTC 
GCCAAAATGG AACATTGTAA AACACTTGTT CTTGACGAGG CTGACAAACT TCTTAGTCAA 
GACTTCCAGG GAATTCTCGA CCGCCTCATC AACTTCCTCC CAAAGGAGCG TCAAGTTATG 
CTTTATTCTG CCACTTTCCC TAACACCGTC ACTTCGTTCA TGCAGAAGCA CATGCACAAG 
CCGTATGAGA TCAACCTCAT GGAGGAGCTT ACTCTTCTTG GTGTCACCCA ATACTATGCC 
TTTGTTCAAG AGAAGCAAAA GGTCCACTGT TTGAATACGT TGTTCCGAAA ACTTCAAATC 
AACCAGTCGA TCATCTTCTG TAACTCTACT CAACGTGTTG AGCTTCTCGC TAAGAAAATC 
ACGGAAATCG GTTACTCGTG CTACTACATT CATTCCAAGA TGGCTCAGAA TCACAGAAAC 
CGTGTCTTCC ATGACTTCCG TCAAGGAAAC TGCAGAAACT TGGTTTGCTC CGATCTTCTC 
ACCCGTGGTA TCGATATCCA GGCTGTCAAT GTTGTCATCA ACTTTGACTT CCCAAGAAAC 
GCCGAGACTT ACCTTCACAG AATCGGTCGC TCTGGACGTT TCGGTCACCT CGGAGTTGCA 
ATCAACCTTA TCACCTACGA AGACCGTCAC ACCCTTCGCC GTATCGAGCA GGAGCTTCGT 
ACTCGCATTG AGCCCATCCC GAAAACGGTT GACCCGAAGC TGTATGTCGC TGACCAGCAG 
CTCGTCGATG CCGCCGATGA GACCACTGCT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_498646.1
CDS	39..1331
Translation	MSGAEQQQIVPANNGDENWKAGLNLPAKDRRFKTADVTDTKGVEFEDFCLGRDLLMGIFEKGWEKPSPIQEASIGVALTGQDILARAKNGTGKTGAYCIPVIEKIQPALKAIQAMVIVPTRELALQTSQICVELSKHIQLKVMVTTGGTDLRDDIMRLNGTVHLVIATPGRILDLMEKGVAKMEHCKTLVLDEADKLLSQDFQGILDRLINFLPKERQVMLYSATFPNTVTSFMQKHMHKPYEINLMEELTLLGVTQYYAFVQEKQKVHCLNTLFRKLQINQSIIFCNSTQRVELLAKKITEIGYSCYYIHSKMAQNHRNRVFHDFRQGNCRNLVCSDLLTRGIDIQAVNVVINFDFPRNAETYLHRIGRSGRFGHLGVAINLITYEDRHTLRRIEQELRTRIEPIPKTVDPKLYVADQQLVDAADETTA

Target ORF information:

RefSeq Version	NM_066245.6
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans ATP-dependent RNA helicase cgh-1 (cgh-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_066245.6

ORF Insert Sequence:

ATGAGTGGAG CGGAGCAACA ACAGATCGTT CCTGCCAACA ATGGCGACGA AAACTGGAAG 
GCTGGTCTCA ATCTTCCAGC AAAAGACAGA AGATTCAAAA CTGCTGATGT CACTGATACG 
AAGGGAGTCG AGTTTGAGGA TTTCTGCCTT GGACGCGATC TTCTCATGGG GATTTTTGAG 
AAAGGATGGG AAAAACCGAG TCCTATTCAA GAAGCATCCA TCGGAGTCGC TCTCACTGGT 
CAAGATATTC TCGCAAGAGC TAAGAACGGA ACAGGAAAAA CCGGAGCTTA TTGTATCCCT 
GTTATCGAGA AGATCCAGCC AGCCCTCAAA GCTATTCAAG CCATGGTCAT TGTCCCAACT 
CGTGAATTGG CTCTCCAAAC TTCACAGATC TGCGTGGAGC TCTCCAAACA CATTCAGCTC 
AAAGTTATGG TCACTACCGG AGGAACTGAT CTCCGTGATG ATATCATGCG TCTAAACGGA 
ACGGTTCACT TGGTCATCGC CACCCCAGGA AGAATTCTCG ATCTTATGGA GAAAGGAGTC 
GCCAAAATGG AACATTGTAA AACACTTGTT CTTGACGAGG CTGACAAACT TCTTAGTCAA 
GACTTCCAGG GAATTCTCGA CCGCCTCATC AACTTCCTCC CAAAGGAGCG TCAAGTTATG 
CTTTATTCTG CCACTTTCCC TAACACCGTC ACTTCGTTCA TGCAGAAGCA CATGCACAAG 
CCGTATGAGA TCAACCTCAT GGAGGAGCTT ACTCTTCTTG GTGTCACCCA ATACTATGCC 
TTTGTTCAAG AGAAGCAAAA GGTCCACTGT TTGAATACGT TGTTCCGAAA ACTTCAAATC 
AACCAGTCGA TCATCTTCTG TAACTCTACT CAACGTGTTG AGCTTCTCGC TAAGAAAATC 
ACGGAAATCG GTTACTCGTG CTACTACATT CATTCCAAGA TGGCTCAGAA TCACAGAAAC 
CGTGTCTTCC ATGACTTCCG TCAAGGAAAC TGCAGAAACT TGGTTTGCTC CGATCTTCTC 
ACCCGTGGTA TCGATATCCA GGCTGTCAAT GTTGTCATCA ACTTTGACTT CCCAAGAAAC 
GCCGAGACTT ACCTTCACAG AATCGGTCGC TCTGGACGTT TCGGTCACCT CGGAGTTGCA 
ATCAACCTTA TCACCTACGA AGACCGTCAC ACCCTTCGCC GTATCGAGCA GGAGCTTCGT 
ACTCGCATTG AGCCCATCCC GAAAACGGTT GACCCGAAGC TGTATGTCGC TGACCAGCAG 
CTCGTCGATG CCGCCGATGA GACCACTGCT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cgh-1 ( NM_066245.5 ) cDNA ORF clone, Caenorhabditis elegans -> NP_498646.1 Caenorhabditis elegans ATP-dependent RNA helicase cgh-1 (cgh-1), partial mRNA.

Price & Availability↑

CloneID	OCa340986
Clone ID Related Accession (Same CDS sequence)	NM_066245.6 , NM_066245.5
Accession Version	NM_066245.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1293bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	ATP-dependent RNA helicase cgh-1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003281). On Jun 26, 2012 this sequence version replaced NM_066245.4. COMPLETENESS: incomplete on both ends.

ATGAGTGGAG CGGAGCAACA ACAGATCGTT CCTGCCAACA ATGGCGACGA AAACTGGAAG 
GCTGGTCTCA ATCTTCCAGC AAAAGACAGA AGATTCAAAA CTGCTGATGT CACTGATACG 
AAGGGAGTCG AGTTTGAGGA TTTCTGCCTT GGACGCGATC TTCTCATGGG GATTTTTGAG 
AAAGGATGGG AAAAACCGAG TCCTATTCAA GAAGCATCCA TCGGAGTCGC TCTCACTGGT 
CAAGATATTC TCGCAAGAGC TAAGAACGGA ACAGGAAAAA CCGGAGCTTA TTGTATCCCT 
GTTATCGAGA AGATCCAGCC AGCCCTCAAA GCTATTCAAG CCATGGTCAT TGTCCCAACT 
CGTGAATTGG CTCTCCAAAC TTCACAGATC TGCGTGGAGC TCTCCAAACA CATTCAGCTC 
AAAGTTATGG TCACTACCGG AGGAACTGAT CTCCGTGATG ATATCATGCG TCTAAACGGA 
ACGGTTCACT TGGTCATCGC CACCCCAGGA AGAATTCTCG ATCTTATGGA GAAAGGAGTC 
GCCAAAATGG AACATTGTAA AACACTTGTT CTTGACGAGG CTGACAAACT TCTTAGTCAA 
GACTTCCAGG GAATTCTCGA CCGCCTCATC AACTTCCTCC CAAAGGAGCG TCAAGTTATG 
CTTTATTCTG CCACTTTCCC TAACACCGTC ACTTCGTTCA TGCAGAAGCA CATGCACAAG 
CCGTATGAGA TCAACCTCAT GGAGGAGCTT ACTCTTCTTG GTGTCACCCA ATACTATGCC 
TTTGTTCAAG AGAAGCAAAA GGTCCACTGT TTGAATACGT TGTTCCGAAA ACTTCAAATC 
AACCAGTCGA TCATCTTCTG TAACTCTACT CAACGTGTTG AGCTTCTCGC TAAGAAAATC 
ACGGAAATCG GTTACTCGTG CTACTACATT CATTCCAAGA TGGCTCAGAA TCACAGAAAC 
CGTGTCTTCC ATGACTTCCG TCAAGGAAAC TGCAGAAACT TGGTTTGCTC CGATCTTCTC 
ACCCGTGGTA TCGATATCCA GGCTGTCAAT GTTGTCATCA ACTTTGACTT CCCAAGAAAC 
GCCGAGACTT ACCTTCACAG AATCGGTCGC TCTGGACGTT TCGGTCACCT CGGAGTTGCA 
ATCAACCTTA TCACCTACGA AGACCGTCAC ACCCTTCGCC GTATCGAGCA GGAGCTTCGT 
ACTCGCATTG AGCCCATCCC GAAAACGGTT GACCCGAAGC TGTATGTCGC TGACCAGCAG 
CTCGTCGATG CCGCCGATGA GACCACTGCT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_498646.1
CDS	1..1293
Translation	MSGAEQQQIVPANNGDENWKAGLNLPAKDRRFKTADVTDTKGVEFEDFCLGRDLLMGIFEKGWEKPSPIQEASIGVALTGQDILARAKNGTGKTGAYCIPVIEKIQPALKAIQAMVIVPTRELALQTSQICVELSKHIQLKVMVTTGGTDLRDDIMRLNGTVHLVIATPGRILDLMEKGVAKMEHCKTLVLDEADKLLSQDFQGILDRLINFLPKERQVMLYSATFPNTVTSFMQKHMHKPYEINLMEELTLLGVTQYYAFVQEKQKVHCLNTLFRKLQINQSIIFCNSTQRVELLAKKITEIGYSCYYIHSKMAQNHRNRVFHDFRQGNCRNLVCSDLLTRGIDIQAVNVVINFDFPRNAETYLHRIGRSGRFGHLGVAINLITYEDRHTLRRIEQELRTRIEPIPKTVDPKLYVADQQLVDAADETTA

Target ORF information:

RefSeq Version	NM_066245.5
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans ATP-dependent RNA helicase cgh-1 (cgh-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_066245.5

ORF Insert Sequence:

ATGAGTGGAG CGGAGCAACA ACAGATCGTT CCTGCCAACA ATGGCGACGA AAACTGGAAG 
GCTGGTCTCA ATCTTCCAGC AAAAGACAGA AGATTCAAAA CTGCTGATGT CACTGATACG 
AAGGGAGTCG AGTTTGAGGA TTTCTGCCTT GGACGCGATC TTCTCATGGG GATTTTTGAG 
AAAGGATGGG AAAAACCGAG TCCTATTCAA GAAGCATCCA TCGGAGTCGC TCTCACTGGT 
CAAGATATTC TCGCAAGAGC TAAGAACGGA ACAGGAAAAA CCGGAGCTTA TTGTATCCCT 
GTTATCGAGA AGATCCAGCC AGCCCTCAAA GCTATTCAAG CCATGGTCAT TGTCCCAACT 
CGTGAATTGG CTCTCCAAAC TTCACAGATC TGCGTGGAGC TCTCCAAACA CATTCAGCTC 
AAAGTTATGG TCACTACCGG AGGAACTGAT CTCCGTGATG ATATCATGCG TCTAAACGGA 
ACGGTTCACT TGGTCATCGC CACCCCAGGA AGAATTCTCG ATCTTATGGA GAAAGGAGTC 
GCCAAAATGG AACATTGTAA AACACTTGTT CTTGACGAGG CTGACAAACT TCTTAGTCAA 
GACTTCCAGG GAATTCTCGA CCGCCTCATC AACTTCCTCC CAAAGGAGCG TCAAGTTATG 
CTTTATTCTG CCACTTTCCC TAACACCGTC ACTTCGTTCA TGCAGAAGCA CATGCACAAG 
CCGTATGAGA TCAACCTCAT GGAGGAGCTT ACTCTTCTTG GTGTCACCCA ATACTATGCC 
TTTGTTCAAG AGAAGCAAAA GGTCCACTGT TTGAATACGT TGTTCCGAAA ACTTCAAATC 
AACCAGTCGA TCATCTTCTG TAACTCTACT CAACGTGTTG AGCTTCTCGC TAAGAAAATC 
ACGGAAATCG GTTACTCGTG CTACTACATT CATTCCAAGA TGGCTCAGAA TCACAGAAAC 
CGTGTCTTCC ATGACTTCCG TCAAGGAAAC TGCAGAAACT TGGTTTGCTC CGATCTTCTC 
ACCCGTGGTA TCGATATCCA GGCTGTCAAT GTTGTCATCA ACTTTGACTT CCCAAGAAAC 
GCCGAGACTT ACCTTCACAG AATCGGTCGC TCTGGACGTT TCGGTCACCT CGGAGTTGCA 
ATCAACCTTA TCACCTACGA AGACCGTCAC ACCCTTCGCC GTATCGAGCA GGAGCTTCGT 
ACTCGCATTG AGCCCATCCC GAAAACGGTT GACCCGAAGC TGTATGTCGC TGACCAGCAG 
CTCGTCGATG CCGCCGATGA GACCACTGCT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.
Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

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cgh-1 cDNA ORF clone, Caenorhabditis elegans

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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