hecw-1 cDNA ORF clone, Caenorhabditis elegans

Gene
Clones
gRNAs

Summary

Gene Symbol	hecw-1
Entrez Gene ID	175438
Full Name	HECT, C2 and WW domain-containing ubiquitin ligase
Gene Type	protein-coding
Organism	Caenorhabditis elegans

mRNA and Protein(s)

mRNA	Protein	Name
NM_065297.1	NP_497698.1	E3 ubiquitin-protein ligase hecw-1
NM_065297.2	NP_497698.1	E3 ubiquitin-protein ligase hecw-1
NM_001330981.2	NP_001317872.1	E3 ubiquitin-protein ligase hecw-1
NM_001330981.1	NP_001317872.1	E3 ubiquitin-protein ligase hecw-1
NM_065296.3	NP_497697.2	E3 ubiquitin-protein ligase hecw-1
NM_065296.4	NP_497697.2	E3 ubiquitin-protein ligase hecw-1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.

Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

HECW-1 functions in a pair of sensory neurons to inhibit P. aeruginosa lawn avoidance behaviour through inhibition of the neuropeptide receptor NPR-1
Title: Natural polymorphisms in C. elegans HECW-1 E3 ligase affect pathogen avoidance behaviour.

The following hecw-1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the hecw-1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa341994	NM_065297.2 Latest version!	Caenorhabditis elegans E3 ubiquitin-protein ligase hecw-1 (hecw-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OCa339943	NM_065296.4 Latest version!	Caenorhabditis elegans E3 ubiquitin-protein ligase hecw-1 (hecw-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OCa340614	NM_001330981.2 Latest version!	Caenorhabditis elegans E3 ubiquitin-protein ligase hecw-1 (hecw-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
View Old Accession Versions >>
OCa341994	NM_065297.1	Caenorhabditis elegans E3 ubiquitin-protein ligase hecw-1 (hecw-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00
OCa340614	NM_001330981.1	Caenorhabditis elegans E3 ubiquitin-protein ligase hecw-1 (hecw-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OCa339943	NM_065296.3	Caenorhabditis elegans E3 ubiquitin-protein ligase hecw-1 (hecw-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

hecw-1 ( NM_065297.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_497698.1 Caenorhabditis elegans E3 ubiquitin-protein ligase hecw-1 (hecw-1), partial mRNA.

Price & Availability↑

CloneID	OCa341994
Clone ID Related Accession (Same CDS sequence)	NM_065297.1 , NM_065297.2
Accession Version	NM_065297.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1104bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	E3 ubiquitin-protein ligase hecw-1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003281). COMPLETENESS: incomplete on both ends.

ATGGGAGACG AGTTCCCATT TCACCATCTG GGATATGTCT GCCTGAATGA GATCTATAAA 
CACTTGACCT TGAAAGAAAT ATTCCAGTTA TCATTTTCCT CTAATCAAAT CAAACGTTCT 
CTTGGAATTG CGAGTATTCC TGTTAAATCA ATTTTTGTCA ATTTTGACTC TCAATACCCG 
AAGATTAAAT TGATTGGAGA GAGTAGTCAA CTGGAATGGG TTTTTGGAAT TCCTGATGGT 
CTGGAATACA CTGATGTCGG GGTTTATAAG ATTGGGCATT TTAAATTTCA GTGCAAAACT 
TCGGGCACCG CATTCTACAC TGAACACTAC GATCTGGAAA ATGCGATGCT AGTTGTGATT 
CGATATATGA TATCCATCTT CAAATGTTCC AATTCAATAA TCACGTATTT ATCGATTGAT 
TTGGGAGTGA TTGAGGATTC GAGAGCCATC TGTGAAAATT TTGTGGCATT CAAAAAAGTC 
GAACGCCTAT CGATTTTTCA AACCAACTAC GGAGTTGAGA GGAATACGTT GAACTTTGCA 
CAGAGTTTTG ATTGGATTTG TGATAACTTG GAAATCAAGA ATATTCATAT TGGAGCGGAT 
GTTTTGGAGC CCAAAATGGT TCAAAAAACC AATGGAGAAT TTGATATTAA ACTTTGTGCA 
CGGAGATTTG AGCAGGTTCT TACAATGGAT CACATCTACT TGCAACATGC CGACTGGATA 
ACATCGGAAG ATCTATTGAA TCTGGACGTT CAGACTGCGA TGCTCATCGA GAATAATCTA 
ACCGAGCAGG ACTTGAACGC GTTCATCAAA CAGTGGCTGA GATCTGACTC TGATAAGTTG 
TGGTGGCTGG AAGTGAAAGG GAAAATGATG TTTAATCGAG AAGAAGTTTT GAAAGATTTG 
GATGTACAAG ATGATGCATA TAGAAGTCTT AATACCAGAT GGTATGTAGC GCGGCGGAGC 
CGCGCCAACC CTTCGGGTAA TGGGTGTGCC CGAAAAAAAA TGGGGTCGTT ACGACCAAAA 
CTGACTGAGT TATGGCCATT TAAAGTTTAT ATCATTTTTG GATCTATATT AATTGTTTTC 
GGGCCTGCGG CCCTCAACCG GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_497698.1
CDS	1..1104
Translation	MGDEFPFHHLGYVCLNEIYKHLTLKEIFQLSFSSNQIKRSLGIASIPVKSIFVNFDSQYPKIKLIGESSQLEWVFGIPDGLEYTDVGVYKIGHFKFQCKTSGTAFYTEHYDLENAMLVVIRYMISIFKCSNSIITYLSIDLGVIEDSRAICENFVAFKKVERLSIFQTNYGVERNTLNFAQSFDWICDNLEIKNIHIGADVLEPKMVQKTNGEFDIKLCARRFEQVLTMDHIYLQHADWITSEDLLNLDVQTAMLIENNLTEQDLNAFIKQWLRSDSDKLWWLEVKGKMMFNREEVLKDLDVQDDAYRSLNTRWYVARRSRANPSGNGCARKKMGSLRPKLTELWPFKVYIIFGSILIVFGPAALNR

Target ORF information:

RefSeq Version	NM_065297.1
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans E3 ubiquitin-protein ligase hecw-1 (hecw-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_065297.1

ORF Insert Sequence:

ATGGGAGACG AGTTCCCATT TCACCATCTG GGATATGTCT GCCTGAATGA GATCTATAAA 
CACTTGACCT TGAAAGAAAT ATTCCAGTTA TCATTTTCCT CTAATCAAAT CAAACGTTCT 
CTTGGAATTG CGAGTATTCC TGTTAAATCA ATTTTTGTCA ATTTTGACTC TCAATACCCG 
AAGATTAAAT TGATTGGAGA GAGTAGTCAA CTGGAATGGG TTTTTGGAAT TCCTGATGGT 
CTGGAATACA CTGATGTCGG GGTTTATAAG ATTGGGCATT TTAAATTTCA GTGCAAAACT 
TCGGGCACCG CATTCTACAC TGAACACTAC GATCTGGAAA ATGCGATGCT AGTTGTGATT 
CGATATATGA TATCCATCTT CAAATGTTCC AATTCAATAA TCACGTATTT ATCGATTGAT 
TTGGGAGTGA TTGAGGATTC GAGAGCCATC TGTGAAAATT TTGTGGCATT CAAAAAAGTC 
GAACGCCTAT CGATTTTTCA AACCAACTAC GGAGTTGAGA GGAATACGTT GAACTTTGCA 
CAGAGTTTTG ATTGGATTTG TGATAACTTG GAAATCAAGA ATATTCATAT TGGAGCGGAT 
GTTTTGGAGC CCAAAATGGT TCAAAAAACC AATGGAGAAT TTGATATTAA ACTTTGTGCA 
CGGAGATTTG AGCAGGTTCT TACAATGGAT CACATCTACT TGCAACATGC CGACTGGATA 
ACATCGGAAG ATCTATTGAA TCTGGACGTT CAGACTGCGA TGCTCATCGA GAATAATCTA 
ACCGAGCAGG ACTTGAACGC GTTCATCAAA CAGTGGCTGA GATCTGACTC TGATAAGTTG 
TGGTGGCTGG AAGTGAAAGG GAAAATGATG TTTAATCGAG AAGAAGTTTT GAAAGATTTG 
GATGTACAAG ATGATGCATA TAGAAGTCTT AATACCAGAT GGTATGTAGC GCGGCGGAGC 
CGCGCCAACC CTTCGGGTAA TGGGTGTGCC CGAAAAAAAA TGGGGTCGTT ACGACCAAAA 
CTGACTGAGT TATGGCCATT TAAAGTTTAT ATCATTTTTG GATCTATATT AATTGTTTTC 
GGGCCTGCGG CCCTCAACCG GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

hecw-1 ( NM_065297.2 ) cDNA ORF clone, Caenorhabditis elegans -> NP_497698.1 Caenorhabditis elegans E3 ubiquitin-protein ligase hecw-1 (hecw-1), partial mRNA.

Price & Availability↑

CloneID	OCa341994
Clone ID Related Accession (Same CDS sequence)	NM_065297.1 , NM_065297.2
Accession Version	NM_065297.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1104bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	E3 ubiquitin-protein ligase hecw-1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003281). On Sep 4, 2019 this sequence version replaced NM_065297.1. COMPLETENESS: incomplete on the 3' end.

ATGGGAGACG AGTTCCCATT TCACCATCTG GGATATGTCT GCCTGAATGA GATCTATAAA 
CACTTGACCT TGAAAGAAAT ATTCCAGTTA TCATTTTCCT CTAATCAAAT CAAACGTTCT 
CTTGGAATTG CGAGTATTCC TGTTAAATCA ATTTTTGTCA ATTTTGACTC TCAATACCCG 
AAGATTAAAT TGATTGGAGA GAGTAGTCAA CTGGAATGGG TTTTTGGAAT TCCTGATGGT 
CTGGAATACA CTGATGTCGG GGTTTATAAG ATTGGGCATT TTAAATTTCA GTGCAAAACT 
TCGGGCACCG CATTCTACAC TGAACACTAC GATCTGGAAA ATGCGATGCT AGTTGTGATT 
CGATATATGA TATCCATCTT CAAATGTTCC AATTCAATAA TCACGTATTT ATCGATTGAT 
TTGGGAGTGA TTGAGGATTC GAGAGCCATC TGTGAAAATT TTGTGGCATT CAAAAAAGTC 
GAACGCCTAT CGATTTTTCA AACCAACTAC GGAGTTGAGA GGAATACGTT GAACTTTGCA 
CAGAGTTTTG ATTGGATTTG TGATAACTTG GAAATCAAGA ATATTCATAT TGGAGCGGAT 
GTTTTGGAGC CCAAAATGGT TCAAAAAACC AATGGAGAAT TTGATATTAA ACTTTGTGCA 
CGGAGATTTG AGCAGGTTCT TACAATGGAT CACATCTACT TGCAACATGC CGACTGGATA 
ACATCGGAAG ATCTATTGAA TCTGGACGTT CAGACTGCGA TGCTCATCGA GAATAATCTA 
ACCGAGCAGG ACTTGAACGC GTTCATCAAA CAGTGGCTGA GATCTGACTC TGATAAGTTG 
TGGTGGCTGG AAGTGAAAGG GAAAATGATG TTTAATCGAG AAGAAGTTTT GAAAGATTTG 
GATGTACAAG ATGATGCATA TAGAAGTCTT AATACCAGAT GGTATGTAGC GCGGCGGAGC 
CGCGCCAACC CTTCGGGTAA TGGGTGTGCC CGAAAAAAAA TGGGGTCGTT ACGACCAAAA 
CTGACTGAGT TATGGCCATT TAAAGTTTAT ATCATTTTTG GATCTATATT AATTGTTTTC 
GGGCCTGCGG CCCTCAACCG GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_497698.1
CDS	59..1162
Translation	MGDEFPFHHLGYVCLNEIYKHLTLKEIFQLSFSSNQIKRSLGIASIPVKSIFVNFDSQYPKIKLIGESSQLEWVFGIPDGLEYTDVGVYKIGHFKFQCKTSGTAFYTEHYDLENAMLVVIRYMISIFKCSNSIITYLSIDLGVIEDSRAICENFVAFKKVERLSIFQTNYGVERNTLNFAQSFDWICDNLEIKNIHIGADVLEPKMVQKTNGEFDIKLCARRFEQVLTMDHIYLQHADWITSEDLLNLDVQTAMLIENNLTEQDLNAFIKQWLRSDSDKLWWLEVKGKMMFNREEVLKDLDVQDDAYRSLNTRWYVARRSRANPSGNGCARKKMGSLRPKLTELWPFKVYIIFGSILIVFGPAALNR

Target ORF information:

RefSeq Version	NM_065297.2
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans E3 ubiquitin-protein ligase hecw-1 (hecw-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_065297.2

ORF Insert Sequence:

ATGGGAGACG AGTTCCCATT TCACCATCTG GGATATGTCT GCCTGAATGA GATCTATAAA 
CACTTGACCT TGAAAGAAAT ATTCCAGTTA TCATTTTCCT CTAATCAAAT CAAACGTTCT 
CTTGGAATTG CGAGTATTCC TGTTAAATCA ATTTTTGTCA ATTTTGACTC TCAATACCCG 
AAGATTAAAT TGATTGGAGA GAGTAGTCAA CTGGAATGGG TTTTTGGAAT TCCTGATGGT 
CTGGAATACA CTGATGTCGG GGTTTATAAG ATTGGGCATT TTAAATTTCA GTGCAAAACT 
TCGGGCACCG CATTCTACAC TGAACACTAC GATCTGGAAA ATGCGATGCT AGTTGTGATT 
CGATATATGA TATCCATCTT CAAATGTTCC AATTCAATAA TCACGTATTT ATCGATTGAT 
TTGGGAGTGA TTGAGGATTC GAGAGCCATC TGTGAAAATT TTGTGGCATT CAAAAAAGTC 
GAACGCCTAT CGATTTTTCA AACCAACTAC GGAGTTGAGA GGAATACGTT GAACTTTGCA 
CAGAGTTTTG ATTGGATTTG TGATAACTTG GAAATCAAGA ATATTCATAT TGGAGCGGAT 
GTTTTGGAGC CCAAAATGGT TCAAAAAACC AATGGAGAAT TTGATATTAA ACTTTGTGCA 
CGGAGATTTG AGCAGGTTCT TACAATGGAT CACATCTACT TGCAACATGC CGACTGGATA 
ACATCGGAAG ATCTATTGAA TCTGGACGTT CAGACTGCGA TGCTCATCGA GAATAATCTA 
ACCGAGCAGG ACTTGAACGC GTTCATCAAA CAGTGGCTGA GATCTGACTC TGATAAGTTG 
TGGTGGCTGG AAGTGAAAGG GAAAATGATG TTTAATCGAG AAGAAGTTTT GAAAGATTTG 
GATGTACAAG ATGATGCATA TAGAAGTCTT AATACCAGAT GGTATGTAGC GCGGCGGAGC 
CGCGCCAACC CTTCGGGTAA TGGGTGTGCC CGAAAAAAAA TGGGGTCGTT ACGACCAAAA 
CTGACTGAGT TATGGCCATT TAAAGTTTAT ATCATTTTTG GATCTATATT AATTGTTTTC 
GGGCCTGCGG CCCTCAACCG GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

hecw-1 ( NM_065296.3 ) cDNA ORF clone, Caenorhabditis elegans -> NP_497697.2 Caenorhabditis elegans E3 ubiquitin-protein ligase hecw-1 (hecw-1), partial mRNA.

Price & Availability↑

CloneID	OCa339943
Clone ID Related Accession (Same CDS sequence)	NM_065296.3 , NM_065296.4
Accession Version	NM_065296.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2625bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	E3 ubiquitin-protein ligase hecw-1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003281). On Jun 26, 2012 this sequence version replaced NM_065296.2. COMPLETENESS: incomplete on both ends.

ATGGATGTCG AAACAGATAG GCCGTCGGGT TCTGAAACGG GAATTTTTGT ATCAAAGTCG 
GCGGTTTATC CGTTTGGAAA CAAGAATGAG GTAGTAAAAG TTTGGTGGAA CATTGACAAG 
CCGGAGAACT GCTTAGACTG GATTGGTCTT TTTGATAATA ATGACTCATC ATTCTACTTG 
GACCAAAAAT CCCTCCGACA ACATATCTCC CCTGTAGTTT TCACATTAAC CTCTAAAAAT 
TTGCTAAATG CTTCTGAAGT ATATTTTGGA TTAATTGATG GAATGACAGG AAGACTGCTG 
GCTAAATCGG AAAATGTAGA GATTTGTAAA TCAGCATCAC TGGTTGTTCA TGAAGTCAAA 
AAACCAGAGA ACATAAATGT CTATCTCAAC ACGAACCACG GGAGAATAGA AATCCCAAAA 
AAGAGGTCGT TTTCCCGGAA GAATGGCTCT TTTCGGAGCT CATTTGAGTT TGATTTTGAT 
GTTGAGGACC TTCATATTTC ATTGGTTGAA AACTCTGAAA AGTTGAATTT CGATCATTTT 
ATTGCTTCGA ATCAACTTCA AAACAATTAC ATCCAATCGA AAAGTTTCTC GCATAGTCAC 
GTCGTGGAAA TTGTCTTCAG TATTGAACCA AAAACCAGTA AAAAATCCGC GCCTGACATA 
ATGGAAATCG CTTCATCAAG TCAAACGCCA CCAGAATCCC ATTGGAAAAC TTATTTGGAC 
GCGAAAAAAC GGAAATTCTA TGTGAACCAT GTCACGAAGG AAACAAGATG GACTAAACCG 
GACACCCTCA ACAACAACCA TATAGAACCA GAGACCCCAG TTCACAAGAG GCTCTCCGAC 
CGATCAGCTT CTCCCAGGAA TAGTTTCATC ACGCCGCGAC GTACAATCAC AGTGAGATCG 
GCGGGGTGTC CAAAAAGCGA TTTGATTCAG TTTTTCCAAC GAGACGAGTT TAAAACCGCC 
TTATACGAGA ATCAAGATGC AATGCAGATT TATAACGAAT GCTCTGTGGT TCGGCATGCG 
ATTCATCGAA TTCAGAAGGA TTTGGATCCA CCATCAAAAT TCGAAAACCA ACCTCTATTC 
GTCCGATTCG TCAACCTATT CGCCGATATC ACGCAGCCTC TGCCCTCCGG CTGGGAATGC 
ATCACAATGA ACAATCGAAC GGTATTTTTG AATCACGCCA ACAAGGAAAC AAGTTTTTAT 
GATCCAAGAA TCAGAAGATT TGAAACAAAA ACTTCACGAC GTGGCAGAAG TGTTCCGAGT 
AGAAGTTCAA CAGCGCACAA AGGAAAAATC GATCACGCAT TGATTAGTAA ATGCGAGGAT 
TTGAGGAAAA TTGCGCAAGA TAATTTCCCG CAGATTGCGG AACGAATAAG CAAAAAGCTC 
ATGCTAATCG AAAGATTTGG AGGACTTGCA GTGGCTAGTT TAGCAAATGA TTTAGATATC 
ACTCTAGCCC TGAGCATGTT GGATTCGAAT ACGGAAAAGC TAGCGGGAGA AGGAGATAAT 
ATTAAAATGT TCTATGAGGA TATGAAAAAG GAGAAGCTTG GCAAGGGGCC GAGTCGGCTT 
TGCTGGAAAG TTTCCAGAGA TCGATTATTG GATGATGCTT TTAGGATTAT TCTGAATGTC 
GATCCATTTG TACTGAAAAA GTCCAGATTG CATATTCGAT TTGAAGGGGA GTTGGCTCTT 
GACTATGGTG GTCTATCACG AGAATTCTTC ATTCTCCTCT CTCGTGAACT ATTTCATCCC 
AAAAATGGAT ATTTTGAGTA CGAAGGAAAC GACTATCATC TGCAATTGAG ACCACGTGGA 
TGTGAAACTG AAAAGGAGAA AAAATGGTTG ATACTTTGTG GAAGAGTCCT GGCATTGGCG 
GTTATACATC GATGCTATAT TGATGTGTTT TTTACGAATG TGTTCTACAA GTCCTTGCAG 
AAGAGACCAG TGACCCTGAT GGACTTTAAA GAATCGGACG CCGAATTCTA CAAAAGCATG 
AATTGGCTTT TGGAAAACGA CGTCGTCGAC CTTGAAATGT CCTTTGTGTA CAGTAGTATG 
GTCAATGGAA AACTAGCCGA ACAAGAACTT GTACCCGGTG GAGAATCACA AATGGTCACT 
GAAGCTAATA AAGCAGAATT TATCGATTTG ATGTGTCAGA AAAAGGCGAT AAGAGGTGTG 
GAGAAGCCAT TGGAGATTCT ATTGACATCT TTTAATCAGA TATTGAATGA TAATCTCTTA 
AACTCACTGG AAAGTTCGGA TTTGAAGCGG ATTCTTAGTG GATCACTGGA ATTGGATCTC 
AATGATTGGA GGACCAATAC GATTTATAAA GGAGGATACT CTGATTGTCA TATAGTCGTC 
GAATGGTTTT GGGAAGTGAT AGAGACGATG ACCAACCAAG AAAGATTTGA TTTGTTGTTG 
TTTGTCACCG GCAGTTCATC AGTTCCTTTT GAAGGATTCT CTGCGCTACG TGGCAATGAG 
GAAATAAGCA AGTTTTGCAT AGAGAAGTGG GGAGATGCCA CGTCGTTTCC CAGGGCTCAT 
ACGTGTTTCA ACAGGCTTCA ACTACCCTCC TATAACACTA AGCAGCAGTT GAAATCAAAA 
CTTCAGCAGG CAATTGTTAA TGGGATGAGC TATTCGATAG AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_497697.2
CDS	1..2625
Translation	MDVETDRPSGSETGIFVSKSAVYPFGNKNEVVKVWWNIDKPENCLDWIGLFDNNDSSFYLDQKSLRQHISPVVFTLTSKNLLNASEVYFGLIDGMTGRLLAKSENVEICKSASLVVHEVKKPENINVYLNTNHGRIEIPKKRSFSRKNGSFRSSFEFDFDVEDLHISLVENSEKLNFDHFIASNQLQNNYIQSKSFSHSHVVEIVFSIEPKTSKKSAPDIMEIASSSQTPPESHWKTYLDAKKRKFYVNHVTKETRWTKPDTLNNNHIEPETPVHKRLSDRSASPRNSFITPRRTITVRSAGCPKSDLIQFFQRDEFKTALYENQDAMQIYNECSVVRHAIHRIQKDLDPPSKFENQPLFVRFVNLFADITQPLPSGWECITMNNRTVFLNHANKETSFYDPRIRRFETKTSRRGRSVPSRSSTAHKGKIDHALISKCEDLRKIAQDNFPQIAERISKKLMLIERFGGLAVASLANDLDITLALSMLDSNTEKLAGEGDNIKMFYEDMKKEKLGKGPSRLCWKVSRDRLLDDAFRIILNVDPFVLKKSRLHIRFEGELALDYGGLSREFFILLSRELFHPKNGYFEYEGNDYHLQLRPRGCETEKEKKWLILCGRVLALAVIHRCYIDVFFTNVFYKSLQKRPVTLMDFKESDAEFYKSMNWLLENDVVDLEMSFVYSSMVNGKLAEQELVPGGESQMVTEANKAEFIDLMCQKKAIRGVEKPLEILLTSFNQILNDNLLNSLESSDLKRILSGSLELDLNDWRTNTIYKGGYSDCHIVVEWFWEVIETMTNQERFDLLLFVTGSSSVPFEGFSALRGNEEISKFCIEKWGDATSFPRAHTCFNRLQLPSYNTKQQLKSKLQQAIVNGMSYSIE

Target ORF information:

RefSeq Version	NM_065296.3
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans E3 ubiquitin-protein ligase hecw-1 (hecw-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_065296.3

ORF Insert Sequence:

ATGGATGTCG AAACAGATAG GCCGTCGGGT TCTGAAACGG GAATTTTTGT ATCAAAGTCG 
GCGGTTTATC CGTTTGGAAA CAAGAATGAG GTAGTAAAAG TTTGGTGGAA CATTGACAAG 
CCGGAGAACT GCTTAGACTG GATTGGTCTT TTTGATAATA ATGACTCATC ATTCTACTTG 
GACCAAAAAT CCCTCCGACA ACATATCTCC CCTGTAGTTT TCACATTAAC CTCTAAAAAT 
TTGCTAAATG CTTCTGAAGT ATATTTTGGA TTAATTGATG GAATGACAGG AAGACTGCTG 
GCTAAATCGG AAAATGTAGA GATTTGTAAA TCAGCATCAC TGGTTGTTCA TGAAGTCAAA 
AAACCAGAGA ACATAAATGT CTATCTCAAC ACGAACCACG GGAGAATAGA AATCCCAAAA 
AAGAGGTCGT TTTCCCGGAA GAATGGCTCT TTTCGGAGCT CATTTGAGTT TGATTTTGAT 
GTTGAGGACC TTCATATTTC ATTGGTTGAA AACTCTGAAA AGTTGAATTT CGATCATTTT 
ATTGCTTCGA ATCAACTTCA AAACAATTAC ATCCAATCGA AAAGTTTCTC GCATAGTCAC 
GTCGTGGAAA TTGTCTTCAG TATTGAACCA AAAACCAGTA AAAAATCCGC GCCTGACATA 
ATGGAAATCG CTTCATCAAG TCAAACGCCA CCAGAATCCC ATTGGAAAAC TTATTTGGAC 
GCGAAAAAAC GGAAATTCTA TGTGAACCAT GTCACGAAGG AAACAAGATG GACTAAACCG 
GACACCCTCA ACAACAACCA TATAGAACCA GAGACCCCAG TTCACAAGAG GCTCTCCGAC 
CGATCAGCTT CTCCCAGGAA TAGTTTCATC ACGCCGCGAC GTACAATCAC AGTGAGATCG 
GCGGGGTGTC CAAAAAGCGA TTTGATTCAG TTTTTCCAAC GAGACGAGTT TAAAACCGCC 
TTATACGAGA ATCAAGATGC AATGCAGATT TATAACGAAT GCTCTGTGGT TCGGCATGCG 
ATTCATCGAA TTCAGAAGGA TTTGGATCCA CCATCAAAAT TCGAAAACCA ACCTCTATTC 
GTCCGATTCG TCAACCTATT CGCCGATATC ACGCAGCCTC TGCCCTCCGG CTGGGAATGC 
ATCACAATGA ACAATCGAAC GGTATTTTTG AATCACGCCA ACAAGGAAAC AAGTTTTTAT 
GATCCAAGAA TCAGAAGATT TGAAACAAAA ACTTCACGAC GTGGCAGAAG TGTTCCGAGT 
AGAAGTTCAA CAGCGCACAA AGGAAAAATC GATCACGCAT TGATTAGTAA ATGCGAGGAT 
TTGAGGAAAA TTGCGCAAGA TAATTTCCCG CAGATTGCGG AACGAATAAG CAAAAAGCTC 
ATGCTAATCG AAAGATTTGG AGGACTTGCA GTGGCTAGTT TAGCAAATGA TTTAGATATC 
ACTCTAGCCC TGAGCATGTT GGATTCGAAT ACGGAAAAGC TAGCGGGAGA AGGAGATAAT 
ATTAAAATGT TCTATGAGGA TATGAAAAAG GAGAAGCTTG GCAAGGGGCC GAGTCGGCTT 
TGCTGGAAAG TTTCCAGAGA TCGATTATTG GATGATGCTT TTAGGATTAT TCTGAATGTC 
GATCCATTTG TACTGAAAAA GTCCAGATTG CATATTCGAT TTGAAGGGGA GTTGGCTCTT 
GACTATGGTG GTCTATCACG AGAATTCTTC ATTCTCCTCT CTCGTGAACT ATTTCATCCC 
AAAAATGGAT ATTTTGAGTA CGAAGGAAAC GACTATCATC TGCAATTGAG ACCACGTGGA 
TGTGAAACTG AAAAGGAGAA AAAATGGTTG ATACTTTGTG GAAGAGTCCT GGCATTGGCG 
GTTATACATC GATGCTATAT TGATGTGTTT TTTACGAATG TGTTCTACAA GTCCTTGCAG 
AAGAGACCAG TGACCCTGAT GGACTTTAAA GAATCGGACG CCGAATTCTA CAAAAGCATG 
AATTGGCTTT TGGAAAACGA CGTCGTCGAC CTTGAAATGT CCTTTGTGTA CAGTAGTATG 
GTCAATGGAA AACTAGCCGA ACAAGAACTT GTACCCGGTG GAGAATCACA AATGGTCACT 
GAAGCTAATA AAGCAGAATT TATCGATTTG ATGTGTCAGA AAAAGGCGAT AAGAGGTGTG 
GAGAAGCCAT TGGAGATTCT ATTGACATCT TTTAATCAGA TATTGAATGA TAATCTCTTA 
AACTCACTGG AAAGTTCGGA TTTGAAGCGG ATTCTTAGTG GATCACTGGA ATTGGATCTC 
AATGATTGGA GGACCAATAC GATTTATAAA GGAGGATACT CTGATTGTCA TATAGTCGTC 
GAATGGTTTT GGGAAGTGAT AGAGACGATG ACCAACCAAG AAAGATTTGA TTTGTTGTTG 
TTTGTCACCG GCAGTTCATC AGTTCCTTTT GAAGGATTCT CTGCGCTACG TGGCAATGAG 
GAAATAAGCA AGTTTTGCAT AGAGAAGTGG GGAGATGCCA CGTCGTTTCC CAGGGCTCAT 
ACGTGTTTCA ACAGGCTTCA ACTACCCTCC TATAACACTA AGCAGCAGTT GAAATCAAAA 
CTTCAGCAGG CAATTGTTAA TGGGATGAGC TATTCGATAG AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

hecw-1 ( NM_065296.4 ) cDNA ORF clone, Caenorhabditis elegans -> NP_497697.2 Caenorhabditis elegans E3 ubiquitin-protein ligase hecw-1 (hecw-1), mRNA.

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CloneID	OCa339943
Clone ID Related Accession (Same CDS sequence)	NM_065296.3 , NM_065296.4
Accession Version	NM_065296.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2625bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	E3 ubiquitin-protein ligase hecw-1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003281). On Sep 4, 2019 this sequence version replaced NM_065296.3.

ATGGATGTCG AAACAGATAG GCCGTCGGGT TCTGAAACGG GAATTTTTGT ATCAAAGTCG 
GCGGTTTATC CGTTTGGAAA CAAGAATGAG GTAGTAAAAG TTTGGTGGAA CATTGACAAG 
CCGGAGAACT GCTTAGACTG GATTGGTCTT TTTGATAATA ATGACTCATC ATTCTACTTG 
GACCAAAAAT CCCTCCGACA ACATATCTCC CCTGTAGTTT TCACATTAAC CTCTAAAAAT 
TTGCTAAATG CTTCTGAAGT ATATTTTGGA TTAATTGATG GAATGACAGG AAGACTGCTG 
GCTAAATCGG AAAATGTAGA GATTTGTAAA TCAGCATCAC TGGTTGTTCA TGAAGTCAAA 
AAACCAGAGA ACATAAATGT CTATCTCAAC ACGAACCACG GGAGAATAGA AATCCCAAAA 
AAGAGGTCGT TTTCCCGGAA GAATGGCTCT TTTCGGAGCT CATTTGAGTT TGATTTTGAT 
GTTGAGGACC TTCATATTTC ATTGGTTGAA AACTCTGAAA AGTTGAATTT CGATCATTTT 
ATTGCTTCGA ATCAACTTCA AAACAATTAC ATCCAATCGA AAAGTTTCTC GCATAGTCAC 
GTCGTGGAAA TTGTCTTCAG TATTGAACCA AAAACCAGTA AAAAATCCGC GCCTGACATA 
ATGGAAATCG CTTCATCAAG TCAAACGCCA CCAGAATCCC ATTGGAAAAC TTATTTGGAC 
GCGAAAAAAC GGAAATTCTA TGTGAACCAT GTCACGAAGG AAACAAGATG GACTAAACCG 
GACACCCTCA ACAACAACCA TATAGAACCA GAGACCCCAG TTCACAAGAG GCTCTCCGAC 
CGATCAGCTT CTCCCAGGAA TAGTTTCATC ACGCCGCGAC GTACAATCAC AGTGAGATCG 
GCGGGGTGTC CAAAAAGCGA TTTGATTCAG TTTTTCCAAC GAGACGAGTT TAAAACCGCC 
TTATACGAGA ATCAAGATGC AATGCAGATT TATAACGAAT GCTCTGTGGT TCGGCATGCG 
ATTCATCGAA TTCAGAAGGA TTTGGATCCA CCATCAAAAT TCGAAAACCA ACCTCTATTC 
GTCCGATTCG TCAACCTATT CGCCGATATC ACGCAGCCTC TGCCCTCCGG CTGGGAATGC 
ATCACAATGA ACAATCGAAC GGTATTTTTG AATCACGCCA ACAAGGAAAC AAGTTTTTAT 
GATCCAAGAA TCAGAAGATT TGAAACAAAA ACTTCACGAC GTGGCAGAAG TGTTCCGAGT 
AGAAGTTCAA CAGCGCACAA AGGAAAAATC GATCACGCAT TGATTAGTAA ATGCGAGGAT 
TTGAGGAAAA TTGCGCAAGA TAATTTCCCG CAGATTGCGG AACGAATAAG CAAAAAGCTC 
ATGCTAATCG AAAGATTTGG AGGACTTGCA GTGGCTAGTT TAGCAAATGA TTTAGATATC 
ACTCTAGCCC TGAGCATGTT GGATTCGAAT ACGGAAAAGC TAGCGGGAGA AGGAGATAAT 
ATTAAAATGT TCTATGAGGA TATGAAAAAG GAGAAGCTTG GCAAGGGGCC GAGTCGGCTT 
TGCTGGAAAG TTTCCAGAGA TCGATTATTG GATGATGCTT TTAGGATTAT TCTGAATGTC 
GATCCATTTG TACTGAAAAA GTCCAGATTG CATATTCGAT TTGAAGGGGA GTTGGCTCTT 
GACTATGGTG GTCTATCACG AGAATTCTTC ATTCTCCTCT CTCGTGAACT ATTTCATCCC 
AAAAATGGAT ATTTTGAGTA CGAAGGAAAC GACTATCATC TGCAATTGAG ACCACGTGGA 
TGTGAAACTG AAAAGGAGAA AAAATGGTTG ATACTTTGTG GAAGAGTCCT GGCATTGGCG 
GTTATACATC GATGCTATAT TGATGTGTTT TTTACGAATG TGTTCTACAA GTCCTTGCAG 
AAGAGACCAG TGACCCTGAT GGACTTTAAA GAATCGGACG CCGAATTCTA CAAAAGCATG 
AATTGGCTTT TGGAAAACGA CGTCGTCGAC CTTGAAATGT CCTTTGTGTA CAGTAGTATG 
GTCAATGGAA AACTAGCCGA ACAAGAACTT GTACCCGGTG GAGAATCACA AATGGTCACT 
GAAGCTAATA AAGCAGAATT TATCGATTTG ATGTGTCAGA AAAAGGCGAT AAGAGGTGTG 
GAGAAGCCAT TGGAGATTCT ATTGACATCT TTTAATCAGA TATTGAATGA TAATCTCTTA 
AACTCACTGG AAAGTTCGGA TTTGAAGCGG ATTCTTAGTG GATCACTGGA ATTGGATCTC 
AATGATTGGA GGACCAATAC GATTTATAAA GGAGGATACT CTGATTGTCA TATAGTCGTC 
GAATGGTTTT GGGAAGTGAT AGAGACGATG ACCAACCAAG AAAGATTTGA TTTGTTGTTG 
TTTGTCACCG GCAGTTCATC AGTTCCTTTT GAAGGATTCT CTGCGCTACG TGGCAATGAG 
GAAATAAGCA AGTTTTGCAT AGAGAAGTGG GGAGATGCCA CGTCGTTTCC CAGGGCTCAT 
ACGTGTTTCA ACAGGCTTCA ACTACCCTCC TATAACACTA AGCAGCAGTT GAAATCAAAA 
CTTCAGCAGG CAATTGTTAA TGGGATGAGC TATTCGATAG AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_497697.2
CDS	678..3302
Translation	MDVETDRPSGSETGIFVSKSAVYPFGNKNEVVKVWWNIDKPENCLDWIGLFDNNDSSFYLDQKSLRQHISPVVFTLTSKNLLNASEVYFGLIDGMTGRLLAKSENVEICKSASLVVHEVKKPENINVYLNTNHGRIEIPKKRSFSRKNGSFRSSFEFDFDVEDLHISLVENSEKLNFDHFIASNQLQNNYIQSKSFSHSHVVEIVFSIEPKTSKKSAPDIMEIASSSQTPPESHWKTYLDAKKRKFYVNHVTKETRWTKPDTLNNNHIEPETPVHKRLSDRSASPRNSFITPRRTITVRSAGCPKSDLIQFFQRDEFKTALYENQDAMQIYNECSVVRHAIHRIQKDLDPPSKFENQPLFVRFVNLFADITQPLPSGWECITMNNRTVFLNHANKETSFYDPRIRRFETKTSRRGRSVPSRSSTAHKGKIDHALISKCEDLRKIAQDNFPQIAERISKKLMLIERFGGLAVASLANDLDITLALSMLDSNTEKLAGEGDNIKMFYEDMKKEKLGKGPSRLCWKVSRDRLLDDAFRIILNVDPFVLKKSRLHIRFEGELALDYGGLSREFFILLSRELFHPKNGYFEYEGNDYHLQLRPRGCETEKEKKWLILCGRVLALAVIHRCYIDVFFTNVFYKSLQKRPVTLMDFKESDAEFYKSMNWLLENDVVDLEMSFVYSSMVNGKLAEQELVPGGESQMVTEANKAEFIDLMCQKKAIRGVEKPLEILLTSFNQILNDNLLNSLESSDLKRILSGSLELDLNDWRTNTIYKGGYSDCHIVVEWFWEVIETMTNQERFDLLLFVTGSSSVPFEGFSALRGNEEISKFCIEKWGDATSFPRAHTCFNRLQLPSYNTKQQLKSKLQQAIVNGMSYSIE

Target ORF information:

RefSeq Version	NM_065296.4
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans E3 ubiquitin-protein ligase hecw-1 (hecw-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_065296.4

ORF Insert Sequence:

ATGGATGTCG AAACAGATAG GCCGTCGGGT TCTGAAACGG GAATTTTTGT ATCAAAGTCG 
GCGGTTTATC CGTTTGGAAA CAAGAATGAG GTAGTAAAAG TTTGGTGGAA CATTGACAAG 
CCGGAGAACT GCTTAGACTG GATTGGTCTT TTTGATAATA ATGACTCATC ATTCTACTTG 
GACCAAAAAT CCCTCCGACA ACATATCTCC CCTGTAGTTT TCACATTAAC CTCTAAAAAT 
TTGCTAAATG CTTCTGAAGT ATATTTTGGA TTAATTGATG GAATGACAGG AAGACTGCTG 
GCTAAATCGG AAAATGTAGA GATTTGTAAA TCAGCATCAC TGGTTGTTCA TGAAGTCAAA 
AAACCAGAGA ACATAAATGT CTATCTCAAC ACGAACCACG GGAGAATAGA AATCCCAAAA 
AAGAGGTCGT TTTCCCGGAA GAATGGCTCT TTTCGGAGCT CATTTGAGTT TGATTTTGAT 
GTTGAGGACC TTCATATTTC ATTGGTTGAA AACTCTGAAA AGTTGAATTT CGATCATTTT 
ATTGCTTCGA ATCAACTTCA AAACAATTAC ATCCAATCGA AAAGTTTCTC GCATAGTCAC 
GTCGTGGAAA TTGTCTTCAG TATTGAACCA AAAACCAGTA AAAAATCCGC GCCTGACATA 
ATGGAAATCG CTTCATCAAG TCAAACGCCA CCAGAATCCC ATTGGAAAAC TTATTTGGAC 
GCGAAAAAAC GGAAATTCTA TGTGAACCAT GTCACGAAGG AAACAAGATG GACTAAACCG 
GACACCCTCA ACAACAACCA TATAGAACCA GAGACCCCAG TTCACAAGAG GCTCTCCGAC 
CGATCAGCTT CTCCCAGGAA TAGTTTCATC ACGCCGCGAC GTACAATCAC AGTGAGATCG 
GCGGGGTGTC CAAAAAGCGA TTTGATTCAG TTTTTCCAAC GAGACGAGTT TAAAACCGCC 
TTATACGAGA ATCAAGATGC AATGCAGATT TATAACGAAT GCTCTGTGGT TCGGCATGCG 
ATTCATCGAA TTCAGAAGGA TTTGGATCCA CCATCAAAAT TCGAAAACCA ACCTCTATTC 
GTCCGATTCG TCAACCTATT CGCCGATATC ACGCAGCCTC TGCCCTCCGG CTGGGAATGC 
ATCACAATGA ACAATCGAAC GGTATTTTTG AATCACGCCA ACAAGGAAAC AAGTTTTTAT 
GATCCAAGAA TCAGAAGATT TGAAACAAAA ACTTCACGAC GTGGCAGAAG TGTTCCGAGT 
AGAAGTTCAA CAGCGCACAA AGGAAAAATC GATCACGCAT TGATTAGTAA ATGCGAGGAT 
TTGAGGAAAA TTGCGCAAGA TAATTTCCCG CAGATTGCGG AACGAATAAG CAAAAAGCTC 
ATGCTAATCG AAAGATTTGG AGGACTTGCA GTGGCTAGTT TAGCAAATGA TTTAGATATC 
ACTCTAGCCC TGAGCATGTT GGATTCGAAT ACGGAAAAGC TAGCGGGAGA AGGAGATAAT 
ATTAAAATGT TCTATGAGGA TATGAAAAAG GAGAAGCTTG GCAAGGGGCC GAGTCGGCTT 
TGCTGGAAAG TTTCCAGAGA TCGATTATTG GATGATGCTT TTAGGATTAT TCTGAATGTC 
GATCCATTTG TACTGAAAAA GTCCAGATTG CATATTCGAT TTGAAGGGGA GTTGGCTCTT 
GACTATGGTG GTCTATCACG AGAATTCTTC ATTCTCCTCT CTCGTGAACT ATTTCATCCC 
AAAAATGGAT ATTTTGAGTA CGAAGGAAAC GACTATCATC TGCAATTGAG ACCACGTGGA 
TGTGAAACTG AAAAGGAGAA AAAATGGTTG ATACTTTGTG GAAGAGTCCT GGCATTGGCG 
GTTATACATC GATGCTATAT TGATGTGTTT TTTACGAATG TGTTCTACAA GTCCTTGCAG 
AAGAGACCAG TGACCCTGAT GGACTTTAAA GAATCGGACG CCGAATTCTA CAAAAGCATG 
AATTGGCTTT TGGAAAACGA CGTCGTCGAC CTTGAAATGT CCTTTGTGTA CAGTAGTATG 
GTCAATGGAA AACTAGCCGA ACAAGAACTT GTACCCGGTG GAGAATCACA AATGGTCACT 
GAAGCTAATA AAGCAGAATT TATCGATTTG ATGTGTCAGA AAAAGGCGAT AAGAGGTGTG 
GAGAAGCCAT TGGAGATTCT ATTGACATCT TTTAATCAGA TATTGAATGA TAATCTCTTA 
AACTCACTGG AAAGTTCGGA TTTGAAGCGG ATTCTTAGTG GATCACTGGA ATTGGATCTC 
AATGATTGGA GGACCAATAC GATTTATAAA GGAGGATACT CTGATTGTCA TATAGTCGTC 
GAATGGTTTT GGGAAGTGAT AGAGACGATG ACCAACCAAG AAAGATTTGA TTTGTTGTTG 
TTTGTCACCG GCAGTTCATC AGTTCCTTTT GAAGGATTCT CTGCGCTACG TGGCAATGAG 
GAAATAAGCA AGTTTTGCAT AGAGAAGTGG GGAGATGCCA CGTCGTTTCC CAGGGCTCAT 
ACGTGTTTCA ACAGGCTTCA ACTACCCTCC TATAACACTA AGCAGCAGTT GAAATCAAAA 
CTTCAGCAGG CAATTGTTAA TGGGATGAGC TATTCGATAG AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

hecw-1 ( NM_001330981.2 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001317872.1 Caenorhabditis elegans E3 ubiquitin-protein ligase hecw-1 (hecw-1), mRNA.

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CloneID	OCa340614
Clone ID Related Accession (Same CDS sequence)	NM_001330981.2 , NM_001330981.1
Accession Version	NM_001330981.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3744bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	E3 ubiquitin-protein ligase hecw-1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003281). On Sep 4, 2019 this sequence version replaced NM_001330981.1.

ATGGGAGACG AGTTCCCATT TCACCATCTG GGATATGTCT GCCTGAATGA GATCTATAAA 
CACTTGACCT TGAAAGAAAT ATTCCAGTTA TCATTTTCCT CTAATCAAAT CAAACGTTCT 
CTTGGAATTG CGAGTATTCC TGTTAAATCA ATTTTTGTCA ATTTTGACTC TCAATACCCG 
AAGATTAAAT TGATTGGAGA GAGTAGTCAA CTGGAATGGG TTTTTGGAAT TCCTGATGGT 
CTGGAATACA CTGATGTCGG GGTTTATAAG ATTGGGCATT TTAAATTTCA GTGCAAAACT 
TCGGGCACCG CATTCTACAC TGAACACTAC GATCTGGAAA ATGCGATGCT AGTTGTGATT 
CGATATATGA TATCCATCTT CAAATGTTCC AATTCAATAA TCACGTATTT ATCGATTGAT 
TTGGGAGTGA TTGAGGATTC GAGAGCCATC TGTGAAAATT TTGTGGCATT CAAAAAAGTC 
GAACGCCTAT CGATTTTTCA AACCAACTAC GGAGTTGAGA GGAATACGTT GAACTTTGCA 
CAGAGTTTTG ATTGGATTTG TGATAACTTG GAAATCAAGA ATATTCATAT TGGAGCGGAT 
GTTTTGGAGC CCAAAATGGT TCAAAAAACC AATGGAGAAT TTGATATTAA ACTTTGTGCA 
CGGAGATTTG AGCAGGTTCT TACAATGGAT CACATCTACT TGCAACATGC CGACTGGATA 
ACATCGGAAG ATCTATTGAA TCTGGACGTT CAGACTGCGA TGCTCATCGA GAATAATCTA 
ACCGAGCAGG ACTTGAACGC GTTCATCAAA CAGTGGCTGA GATCTGACTC TGATAAGTTG 
TGGTGGCTGG AAGTGAAAGG GAAAATGATG TTTAATCGAG AAGAAGTTTT GAAAGATTTG 
GATGTACAAG ATGATGCATA TAGAAGTCTT AATACCAGAT GGTATGTAGC GCGGCGGAGC 
CGCGCCAACC CTTCGGGTAA TGGGTGTGCC CGAAAAAAAA TGGGGTCGTT ACGACCAAAA 
CTGACTGAGT TATGGCCATT TAAAGTTTAT ATCATTTTTG GATCTATATT AATTGTTTTC 
GGGCCTGCGG CCCTCAACCG AATGATCACG CTTCGGAGAA TGGATGTCGA AACAGATAGG 
CCGTCGGGTT CTGAAACGGG AATTTTTGTA TCAAAGTCGG CGGTTTATCC GTTTGGAAAC 
AAGAATGAGG TAGTAAAAGT TTGGTGGAAC ATTGACAAGC CGGAGAACTG CTTAGACTGG 
ATTGGTCTTT TTGATAATAA TGACTCATCA TTCTACTTGG ACCAAAAATC CCTCCGACAA 
CATATCTCCC CTGTAGTTTT CACATTAACC TCTAAAAATT TGCTAAATGC TTCTGAAGTA 
TATTTTGGAT TAATTGATGG AATGACAGGA AGACTGCTGG CTAAATCGGA AAATGTAGAG 
ATTTGTAAAT CAGCATCACT GGTTGTTCAT GAAGTCAAAA AACCAGAGAA CATAAATGTC 
TATCTCAACA CGAACCACGG GAGAATAGAA ATCCCAAAAA AGAGGTCGTT TTCCCGGAAG 
AATGGCTCTT TTCGGAGCTC ATTTGAGTTT GATTTTGATG TTGAGGACCT TCATATTTCA 
TTGGTTGAAA ACTCTGAAAA GTTGAATTTC GATCATTTTA TTGCTTCGAA TCAACTTCAA 
AACAATTACA TCCAATCGAA AAGTTTCTCG CATAGTCACG TCGTGGAAAT TGTCTTCAGT 
ATTGAACCAA AAACCAGTAA AAAATCCGCG CCTGACATAA TGGAAATCGC TTCATCAAGT 
CAAACGCCAC CAGAATCCCA TTGGAAAACT TATTTGGACG CGAAAAAACG GAAATTCTAT 
GTGAACCATG TCACGAAGGA AACAAGATGG ACTAAACCGG ACACCCTCAA CAACAACCAT 
ATAGAACCAG AGACCCCAGT TCACAAGAGG CTCTCCGACC GATCAGCTTC TCCCAGGAAT 
AGTTTCATCA CGCCGCGACG TACAATCACA GTGAGATCGG CGGGGTGTCC AAAAAGCGAT 
TTGATTCAGT TTTTCCAACG AGACGAGTTT AAAACCGCCT TATACGAGAA TCAAGATGCA 
ATGCAGATTT ATAACGAATG CTCTGTGGTT CGGCATGCGA TTCATCGAAT TCAGAAGGAT 
TTGGATCCAC CATCAAAATT CGAAAACCAA CCTCTATTCG TCCGATTCGT CAACCTATTC 
GCCGATATCA CGCAGCCTCT GCCCTCCGGC TGGGAATGCA TCACAATGAA CAATCGAACG 
GTATTTTTGA ATCACGCCAA CAAGGAAACA AGTTTTTATG ATCCAAGAAT CAGAAGATTT 
GAAACAAAAA CTTCACGACG TGGCAGAAGT GTTCCGAGTA GAAGTTCAAC AGCGCACAAA 
GGAAAAATCG ATCACGCATT GATTAGTAAA TGCGAGGATT TGAGGAAAAT TGCGCAAGAT 
AATTTCCCGC AGATTGCGGA ACGAATAAGC AAAAAGCTCA TGCTAATCGA AAGATTTGGA 
GGACTTGCAG TGGCTAGTTT AGCAAATGAT TTAGATATCA CTCTAGCCCT GAGCATGTTG 
GATTCGAATA CGGAAAAGCT AGCGGGAGAA GGAGATAATA TTAAAATGTT CTATGAGGAT 
ATGAAAAAGG AGAAGCTTGG CAAGGGGCCG AGTCGGCTTT GCTGGAAAGT TTCCAGAGAT 
CGATTATTGG ATGATGCTTT TAGGATTATT CTGAATGTCG ATCCATTTGT ACTGAAAAAG 
TCCAGATTGC ATATTCGATT TGAAGGGGAG TTGGCTCTTG ACTATGGTGG TCTATCACGA 
GAATTCTTCA TTCTCCTCTC TCGTGAACTA TTTCATCCCA AAAATGGATA TTTTGAGTAC 
GAAGGAAACG ACTATCATCT GCAATTGAGA CCACGTGGAT GTGAAACTGA AAAGGAGAAA 
AAATGGTTGA TACTTTGTGG AAGAGTCCTG GCATTGGCGG TTATACATCG ATGCTATATT 
GATGTGTTTT TTACGAATGT GTTCTACAAG TCCTTGCAGA AGAGACCAGT GACCCTGATG 
GACTTTAAAG AATCGGACGC CGAATTCTAC AAAAGCATGA ATTGGCTTTT GGAAAACGAC 
GTCGTCGACC TTGAAATGTC CTTTGTGTAC AGTAGTATGG TCAATGGAAA ACTAGCCGAA 
CAAGAACTTG TACCCGGTGG AGAATCACAA ATGGTCACTG AAGCTAATAA AGCAGAATTT 
ATCGATTTGA TGTGTCAGAA AAAGGCGATA AGAGGTGTGG AGAAGCCATT GGAGATTCTA 
TTGACATCTT TTAATCAGAT ATTGAATGAT AATCTCTTAA ACTCACTGGA AAGTTCGGAT 
TTGAAGCGGA TTCTTAGTGG ATCACTGGAA TTGGATCTCA ATGATTGGAG GACCAATACG 
ATTTATAAAG GAGGATACTC TGATTGTCAT ATAGTCGTCG AATGGTTTTG GGAAGTGATA 
GAGACGATGA CCAACCAAGA AAGATTTGAT TTGTTGTTGT TTGTCACCGG CAGTTCATCA 
GTTCCTTTTG AAGGATTCTC TGCGCTACGT GGCAATGAGG AAATAAGCAA GTTTTGCATA 
GAGAAGTGGG GAGATGCCAC GTCGTTTCCC AGGGCTCATA CGTGTTTCAA CAGGCTTCAA 
CTACCCTCCT ATAACACTAA GCAGCAGTTG AAATCAAAAC TTCAGCAGGC AATTGTTAAT 
GGGATGAGCT ATTCGATAGA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001317872.1
CDS	59..3802
Translation	MGDEFPFHHLGYVCLNEIYKHLTLKEIFQLSFSSNQIKRSLGIASIPVKSIFVNFDSQYPKIKLIGESSQLEWVFGIPDGLEYTDVGVYKIGHFKFQCKTSGTAFYTEHYDLENAMLVVIRYMISIFKCSNSIITYLSIDLGVIEDSRAICENFVAFKKVERLSIFQTNYGVERNTLNFAQSFDWICDNLEIKNIHIGADVLEPKMVQKTNGEFDIKLCARRFEQVLTMDHIYLQHADWITSEDLLNLDVQTAMLIENNLTEQDLNAFIKQWLRSDSDKLWWLEVKGKMMFNREEVLKDLDVQDDAYRSLNTRWYVARRSRANPSGNGCARKKMGSLRPKLTELWPFKVYIIFGSILIVFGPAALNRMITLRRMDVETDRPSGSETGIFVSKSAVYPFGNKNEVVKVWWNIDKPENCLDWIGLFDNNDSSFYLDQKSLRQHISPVVFTLTSKNLLNASEVYFGLIDGMTGRLLAKSENVEICKSASLVVHEVKKPENINVYLNTNHGRIEIPKKRSFSRKNGSFRSSFEFDFDVEDLHISLVENSEKLNFDHFIASNQLQNNYIQSKSFSHSHVVEIVFSIEPKTSKKSAPDIMEIASSSQTPPESHWKTYLDAKKRKFYVNHVTKETRWTKPDTLNNNHIEPETPVHKRLSDRSASPRNSFITPRRTITVRSAGCPKSDLIQFFQRDEFKTALYENQDAMQIYNECSVVRHAIHRIQKDLDPPSKFENQPLFVRFVNLFADITQPLPSGWECITMNNRTVFLNHANKETSFYDPRIRRFETKTSRRGRSVPSRSSTAHKGKIDHALISKCEDLRKIAQDNFPQIAERISKKLMLIERFGGLAVASLANDLDITLALSMLDSNTEKLAGEGDNIKMFYEDMKKEKLGKGPSRLCWKVSRDRLLDDAFRIILNVDPFVLKKSRLHIRFEGELALDYGGLSREFFILLSRELFHPKNGYFEYEGNDYHLQLRPRGCETEKEKKWLILCGRVLALAVIHRCYIDVFFTNVFYKSLQKRPVTLMDFKESDAEFYKSMNWLLENDVVDLEMSFVYSSMVNGKLAEQELVPGGESQMVTEANKAEFIDLMCQKKAIRGVEKPLEILLTSFNQILNDNLLNSLESSDLKRILSGSLELDLNDWRTNTIYKGGYSDCHIVVEWFWEVIETMTNQERFDLLLFVTGSSSVPFEGFSALRGNEEISKFCIEKWGDATSFPRAHTCFNRLQLPSYNTKQQLKSKLQQAIVNGMSYSIE

Target ORF information:

RefSeq Version	NM_001330981.2
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans E3 ubiquitin-protein ligase hecw-1 (hecw-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001330981.2

ORF Insert Sequence:

ATGGGAGACG AGTTCCCATT TCACCATCTG GGATATGTCT GCCTGAATGA GATCTATAAA 
CACTTGACCT TGAAAGAAAT ATTCCAGTTA TCATTTTCCT CTAATCAAAT CAAACGTTCT 
CTTGGAATTG CGAGTATTCC TGTTAAATCA ATTTTTGTCA ATTTTGACTC TCAATACCCG 
AAGATTAAAT TGATTGGAGA GAGTAGTCAA CTGGAATGGG TTTTTGGAAT TCCTGATGGT 
CTGGAATACA CTGATGTCGG GGTTTATAAG ATTGGGCATT TTAAATTTCA GTGCAAAACT 
TCGGGCACCG CATTCTACAC TGAACACTAC GATCTGGAAA ATGCGATGCT AGTTGTGATT 
CGATATATGA TATCCATCTT CAAATGTTCC AATTCAATAA TCACGTATTT ATCGATTGAT 
TTGGGAGTGA TTGAGGATTC GAGAGCCATC TGTGAAAATT TTGTGGCATT CAAAAAAGTC 
GAACGCCTAT CGATTTTTCA AACCAACTAC GGAGTTGAGA GGAATACGTT GAACTTTGCA 
CAGAGTTTTG ATTGGATTTG TGATAACTTG GAAATCAAGA ATATTCATAT TGGAGCGGAT 
GTTTTGGAGC CCAAAATGGT TCAAAAAACC AATGGAGAAT TTGATATTAA ACTTTGTGCA 
CGGAGATTTG AGCAGGTTCT TACAATGGAT CACATCTACT TGCAACATGC CGACTGGATA 
ACATCGGAAG ATCTATTGAA TCTGGACGTT CAGACTGCGA TGCTCATCGA GAATAATCTA 
ACCGAGCAGG ACTTGAACGC GTTCATCAAA CAGTGGCTGA GATCTGACTC TGATAAGTTG 
TGGTGGCTGG AAGTGAAAGG GAAAATGATG TTTAATCGAG AAGAAGTTTT GAAAGATTTG 
GATGTACAAG ATGATGCATA TAGAAGTCTT AATACCAGAT GGTATGTAGC GCGGCGGAGC 
CGCGCCAACC CTTCGGGTAA TGGGTGTGCC CGAAAAAAAA TGGGGTCGTT ACGACCAAAA 
CTGACTGAGT TATGGCCATT TAAAGTTTAT ATCATTTTTG GATCTATATT AATTGTTTTC 
GGGCCTGCGG CCCTCAACCG AATGATCACG CTTCGGAGAA TGGATGTCGA AACAGATAGG 
CCGTCGGGTT CTGAAACGGG AATTTTTGTA TCAAAGTCGG CGGTTTATCC GTTTGGAAAC 
AAGAATGAGG TAGTAAAAGT TTGGTGGAAC ATTGACAAGC CGGAGAACTG CTTAGACTGG 
ATTGGTCTTT TTGATAATAA TGACTCATCA TTCTACTTGG ACCAAAAATC CCTCCGACAA 
CATATCTCCC CTGTAGTTTT CACATTAACC TCTAAAAATT TGCTAAATGC TTCTGAAGTA 
TATTTTGGAT TAATTGATGG AATGACAGGA AGACTGCTGG CTAAATCGGA AAATGTAGAG 
ATTTGTAAAT CAGCATCACT GGTTGTTCAT GAAGTCAAAA AACCAGAGAA CATAAATGTC 
TATCTCAACA CGAACCACGG GAGAATAGAA ATCCCAAAAA AGAGGTCGTT TTCCCGGAAG 
AATGGCTCTT TTCGGAGCTC ATTTGAGTTT GATTTTGATG TTGAGGACCT TCATATTTCA 
TTGGTTGAAA ACTCTGAAAA GTTGAATTTC GATCATTTTA TTGCTTCGAA TCAACTTCAA 
AACAATTACA TCCAATCGAA AAGTTTCTCG CATAGTCACG TCGTGGAAAT TGTCTTCAGT 
ATTGAACCAA AAACCAGTAA AAAATCCGCG CCTGACATAA TGGAAATCGC TTCATCAAGT 
CAAACGCCAC CAGAATCCCA TTGGAAAACT TATTTGGACG CGAAAAAACG GAAATTCTAT 
GTGAACCATG TCACGAAGGA AACAAGATGG ACTAAACCGG ACACCCTCAA CAACAACCAT 
ATAGAACCAG AGACCCCAGT TCACAAGAGG CTCTCCGACC GATCAGCTTC TCCCAGGAAT 
AGTTTCATCA CGCCGCGACG TACAATCACA GTGAGATCGG CGGGGTGTCC AAAAAGCGAT 
TTGATTCAGT TTTTCCAACG AGACGAGTTT AAAACCGCCT TATACGAGAA TCAAGATGCA 
ATGCAGATTT ATAACGAATG CTCTGTGGTT CGGCATGCGA TTCATCGAAT TCAGAAGGAT 
TTGGATCCAC CATCAAAATT CGAAAACCAA CCTCTATTCG TCCGATTCGT CAACCTATTC 
GCCGATATCA CGCAGCCTCT GCCCTCCGGC TGGGAATGCA TCACAATGAA CAATCGAACG 
GTATTTTTGA ATCACGCCAA CAAGGAAACA AGTTTTTATG ATCCAAGAAT CAGAAGATTT 
GAAACAAAAA CTTCACGACG TGGCAGAAGT GTTCCGAGTA GAAGTTCAAC AGCGCACAAA 
GGAAAAATCG ATCACGCATT GATTAGTAAA TGCGAGGATT TGAGGAAAAT TGCGCAAGAT 
AATTTCCCGC AGATTGCGGA ACGAATAAGC AAAAAGCTCA TGCTAATCGA AAGATTTGGA 
GGACTTGCAG TGGCTAGTTT AGCAAATGAT TTAGATATCA CTCTAGCCCT GAGCATGTTG 
GATTCGAATA CGGAAAAGCT AGCGGGAGAA GGAGATAATA TTAAAATGTT CTATGAGGAT 
ATGAAAAAGG AGAAGCTTGG CAAGGGGCCG AGTCGGCTTT GCTGGAAAGT TTCCAGAGAT 
CGATTATTGG ATGATGCTTT TAGGATTATT CTGAATGTCG ATCCATTTGT ACTGAAAAAG 
TCCAGATTGC ATATTCGATT TGAAGGGGAG TTGGCTCTTG ACTATGGTGG TCTATCACGA 
GAATTCTTCA TTCTCCTCTC TCGTGAACTA TTTCATCCCA AAAATGGATA TTTTGAGTAC 
GAAGGAAACG ACTATCATCT GCAATTGAGA CCACGTGGAT GTGAAACTGA AAAGGAGAAA 
AAATGGTTGA TACTTTGTGG AAGAGTCCTG GCATTGGCGG TTATACATCG ATGCTATATT 
GATGTGTTTT TTACGAATGT GTTCTACAAG TCCTTGCAGA AGAGACCAGT GACCCTGATG 
GACTTTAAAG AATCGGACGC CGAATTCTAC AAAAGCATGA ATTGGCTTTT GGAAAACGAC 
GTCGTCGACC TTGAAATGTC CTTTGTGTAC AGTAGTATGG TCAATGGAAA ACTAGCCGAA 
CAAGAACTTG TACCCGGTGG AGAATCACAA ATGGTCACTG AAGCTAATAA AGCAGAATTT 
ATCGATTTGA TGTGTCAGAA AAAGGCGATA AGAGGTGTGG AGAAGCCATT GGAGATTCTA 
TTGACATCTT TTAATCAGAT ATTGAATGAT AATCTCTTAA ACTCACTGGA AAGTTCGGAT 
TTGAAGCGGA TTCTTAGTGG ATCACTGGAA TTGGATCTCA ATGATTGGAG GACCAATACG 
ATTTATAAAG GAGGATACTC TGATTGTCAT ATAGTCGTCG AATGGTTTTG GGAAGTGATA 
GAGACGATGA CCAACCAAGA AAGATTTGAT TTGTTGTTGT TTGTCACCGG CAGTTCATCA 
GTTCCTTTTG AAGGATTCTC TGCGCTACGT GGCAATGAGG AAATAAGCAA GTTTTGCATA 
GAGAAGTGGG GAGATGCCAC GTCGTTTCCC AGGGCTCATA CGTGTTTCAA CAGGCTTCAA 
CTACCCTCCT ATAACACTAA GCAGCAGTTG AAATCAAAAC TTCAGCAGGC AATTGTTAAT 
GGGATGAGCT ATTCGATAGA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

hecw-1 ( NM_001330981.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001317872.1 Caenorhabditis elegans E3 ubiquitin-protein ligase hecw-1 (hecw-1), partial mRNA.

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CloneID	OCa340614
Clone ID Related Accession (Same CDS sequence)	NM_001330981.2 , NM_001330981.1
Accession Version	NM_001330981.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3744bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	E3 ubiquitin-protein ligase hecw-1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003281). COMPLETENESS: incomplete on both ends.

ATGGGAGACG AGTTCCCATT TCACCATCTG GGATATGTCT GCCTGAATGA GATCTATAAA 
CACTTGACCT TGAAAGAAAT ATTCCAGTTA TCATTTTCCT CTAATCAAAT CAAACGTTCT 
CTTGGAATTG CGAGTATTCC TGTTAAATCA ATTTTTGTCA ATTTTGACTC TCAATACCCG 
AAGATTAAAT TGATTGGAGA GAGTAGTCAA CTGGAATGGG TTTTTGGAAT TCCTGATGGT 
CTGGAATACA CTGATGTCGG GGTTTATAAG ATTGGGCATT TTAAATTTCA GTGCAAAACT 
TCGGGCACCG CATTCTACAC TGAACACTAC GATCTGGAAA ATGCGATGCT AGTTGTGATT 
CGATATATGA TATCCATCTT CAAATGTTCC AATTCAATAA TCACGTATTT ATCGATTGAT 
TTGGGAGTGA TTGAGGATTC GAGAGCCATC TGTGAAAATT TTGTGGCATT CAAAAAAGTC 
GAACGCCTAT CGATTTTTCA AACCAACTAC GGAGTTGAGA GGAATACGTT GAACTTTGCA 
CAGAGTTTTG ATTGGATTTG TGATAACTTG GAAATCAAGA ATATTCATAT TGGAGCGGAT 
GTTTTGGAGC CCAAAATGGT TCAAAAAACC AATGGAGAAT TTGATATTAA ACTTTGTGCA 
CGGAGATTTG AGCAGGTTCT TACAATGGAT CACATCTACT TGCAACATGC CGACTGGATA 
ACATCGGAAG ATCTATTGAA TCTGGACGTT CAGACTGCGA TGCTCATCGA GAATAATCTA 
ACCGAGCAGG ACTTGAACGC GTTCATCAAA CAGTGGCTGA GATCTGACTC TGATAAGTTG 
TGGTGGCTGG AAGTGAAAGG GAAAATGATG TTTAATCGAG AAGAAGTTTT GAAAGATTTG 
GATGTACAAG ATGATGCATA TAGAAGTCTT AATACCAGAT GGTATGTAGC GCGGCGGAGC 
CGCGCCAACC CTTCGGGTAA TGGGTGTGCC CGAAAAAAAA TGGGGTCGTT ACGACCAAAA 
CTGACTGAGT TATGGCCATT TAAAGTTTAT ATCATTTTTG GATCTATATT AATTGTTTTC 
GGGCCTGCGG CCCTCAACCG AATGATCACG CTTCGGAGAA TGGATGTCGA AACAGATAGG 
CCGTCGGGTT CTGAAACGGG AATTTTTGTA TCAAAGTCGG CGGTTTATCC GTTTGGAAAC 
AAGAATGAGG TAGTAAAAGT TTGGTGGAAC ATTGACAAGC CGGAGAACTG CTTAGACTGG 
ATTGGTCTTT TTGATAATAA TGACTCATCA TTCTACTTGG ACCAAAAATC CCTCCGACAA 
CATATCTCCC CTGTAGTTTT CACATTAACC TCTAAAAATT TGCTAAATGC TTCTGAAGTA 
TATTTTGGAT TAATTGATGG AATGACAGGA AGACTGCTGG CTAAATCGGA AAATGTAGAG 
ATTTGTAAAT CAGCATCACT GGTTGTTCAT GAAGTCAAAA AACCAGAGAA CATAAATGTC 
TATCTCAACA CGAACCACGG GAGAATAGAA ATCCCAAAAA AGAGGTCGTT TTCCCGGAAG 
AATGGCTCTT TTCGGAGCTC ATTTGAGTTT GATTTTGATG TTGAGGACCT TCATATTTCA 
TTGGTTGAAA ACTCTGAAAA GTTGAATTTC GATCATTTTA TTGCTTCGAA TCAACTTCAA 
AACAATTACA TCCAATCGAA AAGTTTCTCG CATAGTCACG TCGTGGAAAT TGTCTTCAGT 
ATTGAACCAA AAACCAGTAA AAAATCCGCG CCTGACATAA TGGAAATCGC TTCATCAAGT 
CAAACGCCAC CAGAATCCCA TTGGAAAACT TATTTGGACG CGAAAAAACG GAAATTCTAT 
GTGAACCATG TCACGAAGGA AACAAGATGG ACTAAACCGG ACACCCTCAA CAACAACCAT 
ATAGAACCAG AGACCCCAGT TCACAAGAGG CTCTCCGACC GATCAGCTTC TCCCAGGAAT 
AGTTTCATCA CGCCGCGACG TACAATCACA GTGAGATCGG CGGGGTGTCC AAAAAGCGAT 
TTGATTCAGT TTTTCCAACG AGACGAGTTT AAAACCGCCT TATACGAGAA TCAAGATGCA 
ATGCAGATTT ATAACGAATG CTCTGTGGTT CGGCATGCGA TTCATCGAAT TCAGAAGGAT 
TTGGATCCAC CATCAAAATT CGAAAACCAA CCTCTATTCG TCCGATTCGT CAACCTATTC 
GCCGATATCA CGCAGCCTCT GCCCTCCGGC TGGGAATGCA TCACAATGAA CAATCGAACG 
GTATTTTTGA ATCACGCCAA CAAGGAAACA AGTTTTTATG ATCCAAGAAT CAGAAGATTT 
GAAACAAAAA CTTCACGACG TGGCAGAAGT GTTCCGAGTA GAAGTTCAAC AGCGCACAAA 
GGAAAAATCG ATCACGCATT GATTAGTAAA TGCGAGGATT TGAGGAAAAT TGCGCAAGAT 
AATTTCCCGC AGATTGCGGA ACGAATAAGC AAAAAGCTCA TGCTAATCGA AAGATTTGGA 
GGACTTGCAG TGGCTAGTTT AGCAAATGAT TTAGATATCA CTCTAGCCCT GAGCATGTTG 
GATTCGAATA CGGAAAAGCT AGCGGGAGAA GGAGATAATA TTAAAATGTT CTATGAGGAT 
ATGAAAAAGG AGAAGCTTGG CAAGGGGCCG AGTCGGCTTT GCTGGAAAGT TTCCAGAGAT 
CGATTATTGG ATGATGCTTT TAGGATTATT CTGAATGTCG ATCCATTTGT ACTGAAAAAG 
TCCAGATTGC ATATTCGATT TGAAGGGGAG TTGGCTCTTG ACTATGGTGG TCTATCACGA 
GAATTCTTCA TTCTCCTCTC TCGTGAACTA TTTCATCCCA AAAATGGATA TTTTGAGTAC 
GAAGGAAACG ACTATCATCT GCAATTGAGA CCACGTGGAT GTGAAACTGA AAAGGAGAAA 
AAATGGTTGA TACTTTGTGG AAGAGTCCTG GCATTGGCGG TTATACATCG ATGCTATATT 
GATGTGTTTT TTACGAATGT GTTCTACAAG TCCTTGCAGA AGAGACCAGT GACCCTGATG 
GACTTTAAAG AATCGGACGC CGAATTCTAC AAAAGCATGA ATTGGCTTTT GGAAAACGAC 
GTCGTCGACC TTGAAATGTC CTTTGTGTAC AGTAGTATGG TCAATGGAAA ACTAGCCGAA 
CAAGAACTTG TACCCGGTGG AGAATCACAA ATGGTCACTG AAGCTAATAA AGCAGAATTT 
ATCGATTTGA TGTGTCAGAA AAAGGCGATA AGAGGTGTGG AGAAGCCATT GGAGATTCTA 
TTGACATCTT TTAATCAGAT ATTGAATGAT AATCTCTTAA ACTCACTGGA AAGTTCGGAT 
TTGAAGCGGA TTCTTAGTGG ATCACTGGAA TTGGATCTCA ATGATTGGAG GACCAATACG 
ATTTATAAAG GAGGATACTC TGATTGTCAT ATAGTCGTCG AATGGTTTTG GGAAGTGATA 
GAGACGATGA CCAACCAAGA AAGATTTGAT TTGTTGTTGT TTGTCACCGG CAGTTCATCA 
GTTCCTTTTG AAGGATTCTC TGCGCTACGT GGCAATGAGG AAATAAGCAA GTTTTGCATA 
GAGAAGTGGG GAGATGCCAC GTCGTTTCCC AGGGCTCATA CGTGTTTCAA CAGGCTTCAA 
CTACCCTCCT ATAACACTAA GCAGCAGTTG AAATCAAAAC TTCAGCAGGC AATTGTTAAT 
GGGATGAGCT ATTCGATAGA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001317872.1
CDS	1..3744
Translation	MGDEFPFHHLGYVCLNEIYKHLTLKEIFQLSFSSNQIKRSLGIASIPVKSIFVNFDSQYPKIKLIGESSQLEWVFGIPDGLEYTDVGVYKIGHFKFQCKTSGTAFYTEHYDLENAMLVVIRYMISIFKCSNSIITYLSIDLGVIEDSRAICENFVAFKKVERLSIFQTNYGVERNTLNFAQSFDWICDNLEIKNIHIGADVLEPKMVQKTNGEFDIKLCARRFEQVLTMDHIYLQHADWITSEDLLNLDVQTAMLIENNLTEQDLNAFIKQWLRSDSDKLWWLEVKGKMMFNREEVLKDLDVQDDAYRSLNTRWYVARRSRANPSGNGCARKKMGSLRPKLTELWPFKVYIIFGSILIVFGPAALNRMITLRRMDVETDRPSGSETGIFVSKSAVYPFGNKNEVVKVWWNIDKPENCLDWIGLFDNNDSSFYLDQKSLRQHISPVVFTLTSKNLLNASEVYFGLIDGMTGRLLAKSENVEICKSASLVVHEVKKPENINVYLNTNHGRIEIPKKRSFSRKNGSFRSSFEFDFDVEDLHISLVENSEKLNFDHFIASNQLQNNYIQSKSFSHSHVVEIVFSIEPKTSKKSAPDIMEIASSSQTPPESHWKTYLDAKKRKFYVNHVTKETRWTKPDTLNNNHIEPETPVHKRLSDRSASPRNSFITPRRTITVRSAGCPKSDLIQFFQRDEFKTALYENQDAMQIYNECSVVRHAIHRIQKDLDPPSKFENQPLFVRFVNLFADITQPLPSGWECITMNNRTVFLNHANKETSFYDPRIRRFETKTSRRGRSVPSRSSTAHKGKIDHALISKCEDLRKIAQDNFPQIAERISKKLMLIERFGGLAVASLANDLDITLALSMLDSNTEKLAGEGDNIKMFYEDMKKEKLGKGPSRLCWKVSRDRLLDDAFRIILNVDPFVLKKSRLHIRFEGELALDYGGLSREFFILLSRELFHPKNGYFEYEGNDYHLQLRPRGCETEKEKKWLILCGRVLALAVIHRCYIDVFFTNVFYKSLQKRPVTLMDFKESDAEFYKSMNWLLENDVVDLEMSFVYSSMVNGKLAEQELVPGGESQMVTEANKAEFIDLMCQKKAIRGVEKPLEILLTSFNQILNDNLLNSLESSDLKRILSGSLELDLNDWRTNTIYKGGYSDCHIVVEWFWEVIETMTNQERFDLLLFVTGSSSVPFEGFSALRGNEEISKFCIEKWGDATSFPRAHTCFNRLQLPSYNTKQQLKSKLQQAIVNGMSYSIE

Target ORF information:

RefSeq Version	NM_001330981.1
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans E3 ubiquitin-protein ligase hecw-1 (hecw-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001330981.1

ORF Insert Sequence:

ATGGGAGACG AGTTCCCATT TCACCATCTG GGATATGTCT GCCTGAATGA GATCTATAAA 
CACTTGACCT TGAAAGAAAT ATTCCAGTTA TCATTTTCCT CTAATCAAAT CAAACGTTCT 
CTTGGAATTG CGAGTATTCC TGTTAAATCA ATTTTTGTCA ATTTTGACTC TCAATACCCG 
AAGATTAAAT TGATTGGAGA GAGTAGTCAA CTGGAATGGG TTTTTGGAAT TCCTGATGGT 
CTGGAATACA CTGATGTCGG GGTTTATAAG ATTGGGCATT TTAAATTTCA GTGCAAAACT 
TCGGGCACCG CATTCTACAC TGAACACTAC GATCTGGAAA ATGCGATGCT AGTTGTGATT 
CGATATATGA TATCCATCTT CAAATGTTCC AATTCAATAA TCACGTATTT ATCGATTGAT 
TTGGGAGTGA TTGAGGATTC GAGAGCCATC TGTGAAAATT TTGTGGCATT CAAAAAAGTC 
GAACGCCTAT CGATTTTTCA AACCAACTAC GGAGTTGAGA GGAATACGTT GAACTTTGCA 
CAGAGTTTTG ATTGGATTTG TGATAACTTG GAAATCAAGA ATATTCATAT TGGAGCGGAT 
GTTTTGGAGC CCAAAATGGT TCAAAAAACC AATGGAGAAT TTGATATTAA ACTTTGTGCA 
CGGAGATTTG AGCAGGTTCT TACAATGGAT CACATCTACT TGCAACATGC CGACTGGATA 
ACATCGGAAG ATCTATTGAA TCTGGACGTT CAGACTGCGA TGCTCATCGA GAATAATCTA 
ACCGAGCAGG ACTTGAACGC GTTCATCAAA CAGTGGCTGA GATCTGACTC TGATAAGTTG 
TGGTGGCTGG AAGTGAAAGG GAAAATGATG TTTAATCGAG AAGAAGTTTT GAAAGATTTG 
GATGTACAAG ATGATGCATA TAGAAGTCTT AATACCAGAT GGTATGTAGC GCGGCGGAGC 
CGCGCCAACC CTTCGGGTAA TGGGTGTGCC CGAAAAAAAA TGGGGTCGTT ACGACCAAAA 
CTGACTGAGT TATGGCCATT TAAAGTTTAT ATCATTTTTG GATCTATATT AATTGTTTTC 
GGGCCTGCGG CCCTCAACCG AATGATCACG CTTCGGAGAA TGGATGTCGA AACAGATAGG 
CCGTCGGGTT CTGAAACGGG AATTTTTGTA TCAAAGTCGG CGGTTTATCC GTTTGGAAAC 
AAGAATGAGG TAGTAAAAGT TTGGTGGAAC ATTGACAAGC CGGAGAACTG CTTAGACTGG 
ATTGGTCTTT TTGATAATAA TGACTCATCA TTCTACTTGG ACCAAAAATC CCTCCGACAA 
CATATCTCCC CTGTAGTTTT CACATTAACC TCTAAAAATT TGCTAAATGC TTCTGAAGTA 
TATTTTGGAT TAATTGATGG AATGACAGGA AGACTGCTGG CTAAATCGGA AAATGTAGAG 
ATTTGTAAAT CAGCATCACT GGTTGTTCAT GAAGTCAAAA AACCAGAGAA CATAAATGTC 
TATCTCAACA CGAACCACGG GAGAATAGAA ATCCCAAAAA AGAGGTCGTT TTCCCGGAAG 
AATGGCTCTT TTCGGAGCTC ATTTGAGTTT GATTTTGATG TTGAGGACCT TCATATTTCA 
TTGGTTGAAA ACTCTGAAAA GTTGAATTTC GATCATTTTA TTGCTTCGAA TCAACTTCAA 
AACAATTACA TCCAATCGAA AAGTTTCTCG CATAGTCACG TCGTGGAAAT TGTCTTCAGT 
ATTGAACCAA AAACCAGTAA AAAATCCGCG CCTGACATAA TGGAAATCGC TTCATCAAGT 
CAAACGCCAC CAGAATCCCA TTGGAAAACT TATTTGGACG CGAAAAAACG GAAATTCTAT 
GTGAACCATG TCACGAAGGA AACAAGATGG ACTAAACCGG ACACCCTCAA CAACAACCAT 
ATAGAACCAG AGACCCCAGT TCACAAGAGG CTCTCCGACC GATCAGCTTC TCCCAGGAAT 
AGTTTCATCA CGCCGCGACG TACAATCACA GTGAGATCGG CGGGGTGTCC AAAAAGCGAT 
TTGATTCAGT TTTTCCAACG AGACGAGTTT AAAACCGCCT TATACGAGAA TCAAGATGCA 
ATGCAGATTT ATAACGAATG CTCTGTGGTT CGGCATGCGA TTCATCGAAT TCAGAAGGAT 
TTGGATCCAC CATCAAAATT CGAAAACCAA CCTCTATTCG TCCGATTCGT CAACCTATTC 
GCCGATATCA CGCAGCCTCT GCCCTCCGGC TGGGAATGCA TCACAATGAA CAATCGAACG 
GTATTTTTGA ATCACGCCAA CAAGGAAACA AGTTTTTATG ATCCAAGAAT CAGAAGATTT 
GAAACAAAAA CTTCACGACG TGGCAGAAGT GTTCCGAGTA GAAGTTCAAC AGCGCACAAA 
GGAAAAATCG ATCACGCATT GATTAGTAAA TGCGAGGATT TGAGGAAAAT TGCGCAAGAT 
AATTTCCCGC AGATTGCGGA ACGAATAAGC AAAAAGCTCA TGCTAATCGA AAGATTTGGA 
GGACTTGCAG TGGCTAGTTT AGCAAATGAT TTAGATATCA CTCTAGCCCT GAGCATGTTG 
GATTCGAATA CGGAAAAGCT AGCGGGAGAA GGAGATAATA TTAAAATGTT CTATGAGGAT 
ATGAAAAAGG AGAAGCTTGG CAAGGGGCCG AGTCGGCTTT GCTGGAAAGT TTCCAGAGAT 
CGATTATTGG ATGATGCTTT TAGGATTATT CTGAATGTCG ATCCATTTGT ACTGAAAAAG 
TCCAGATTGC ATATTCGATT TGAAGGGGAG TTGGCTCTTG ACTATGGTGG TCTATCACGA 
GAATTCTTCA TTCTCCTCTC TCGTGAACTA TTTCATCCCA AAAATGGATA TTTTGAGTAC 
GAAGGAAACG ACTATCATCT GCAATTGAGA CCACGTGGAT GTGAAACTGA AAAGGAGAAA 
AAATGGTTGA TACTTTGTGG AAGAGTCCTG GCATTGGCGG TTATACATCG ATGCTATATT 
GATGTGTTTT TTACGAATGT GTTCTACAAG TCCTTGCAGA AGAGACCAGT GACCCTGATG 
GACTTTAAAG AATCGGACGC CGAATTCTAC AAAAGCATGA ATTGGCTTTT GGAAAACGAC 
GTCGTCGACC TTGAAATGTC CTTTGTGTAC AGTAGTATGG TCAATGGAAA ACTAGCCGAA 
CAAGAACTTG TACCCGGTGG AGAATCACAA ATGGTCACTG AAGCTAATAA AGCAGAATTT 
ATCGATTTGA TGTGTCAGAA AAAGGCGATA AGAGGTGTGG AGAAGCCATT GGAGATTCTA 
TTGACATCTT TTAATCAGAT ATTGAATGAT AATCTCTTAA ACTCACTGGA AAGTTCGGAT 
TTGAAGCGGA TTCTTAGTGG ATCACTGGAA TTGGATCTCA ATGATTGGAG GACCAATACG 
ATTTATAAAG GAGGATACTC TGATTGTCAT ATAGTCGTCG AATGGTTTTG GGAAGTGATA 
GAGACGATGA CCAACCAAGA AAGATTTGAT TTGTTGTTGT TTGTCACCGG CAGTTCATCA 
GTTCCTTTTG AAGGATTCTC TGCGCTACGT GGCAATGAGG AAATAAGCAA GTTTTGCATA 
GAGAAGTGGG GAGATGCCAC GTCGTTTCCC AGGGCTCATA CGTGTTTCAA CAGGCTTCAA 
CTACCCTCCT ATAACACTAA GCAGCAGTTG AAATCAAAAC TTCAGCAGGC AATTGTTAAT 
GGGATGAGCT ATTCGATAGA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.
Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

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hecw-1 cDNA ORF clone, Caenorhabditis elegans

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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