ire-1 cDNA ORF clone, Caenorhabditis elegans

Gene
Clones
gRNAs

Summary

Gene Symbol	ire-1
Entrez Gene ID	174305
Full Name	IRE1 kinase related
Gene Type	protein-coding
Organism	Caenorhabditis elegans

mRNA and Protein(s)

mRNA	Protein	Name
NM_001267206.2	NP_001254135.1	Serine/threonine-protein kinase
NM_001267207.1	NP_001254136.1	Serine/threonine-protein kinase
NM_001267206.1	NP_001254135.1	Serine/threonine-protein kinase
NM_001267207.2	NP_001254136.1	Serine/threonine-protein kinase

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Ern1	NP_001178855.1
	ERN1	XP_001109583.2
Canis lupus familiaris (dog)	ERN1	XP_853409.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004176	XP_562694.4
Mus musculus (house mouse)	Ern1	NP_076402.1
Xenopus tropicalis (tropical clawed frog)	ern2	NP_001072279.1
Homo sapiens (human)	ERN1	NP_001424.3
Pan troglodytes (chimpanzee)	ERN1	XP_511585.3
Danio rerio (zebrafish)	ern2	XP_001919350.3
Drosophila melanogaster (fruit fly)	Ire1	NP_001097839.1
Bos taurus (cattle)	ERN1	XP_002696144.1
Gallus gallus (chicken)	LOC417414	XP_004946240.1
Caenorhabditis elegans (roundworm)	ire-1	NP_001254135.1

Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.

Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Enhanced study of the role of IIS pathway and epigenetic mechanisms that regulate aging may facilitate progressive prevention and treatment of human age-related diseases.
Title: Insulin/insulin-like growth factor-1 signalling (IIS) based regulation of lifespan across species.

IRE-1 is essential for dendrite morphogenesis. Reducing Insulin/IGF1 signaling can restore normal dendrite arborization in the complete absence of functional IRE-1.
Title: Reduced Insulin/Insulin-Like Growth Factor Receptor Signaling Mitigates Defective Dendrite Morphogenesis in Mutants of the ER Stress Sensor IRE-1.

vitamin-D3-induced lifespan extension requires the stress response pathway genes skn-1, ire-1, and xbp-1.
Title: Vitamin D Promotes Protein Homeostasis and Longevity via the Stress Response Pathway Genes skn-1, ire-1, and xbp-1.

IRE-1 has an ancient function as a cytoplasmic sentinel that activates p38 and SKN-1(Nrf2). csteine modifications induced by ROS signals can direct proteins to adopt unexpected functions and may coordinate many cellular processes.
Title: Cysteine Sulfenylation Directs IRE-1 to Activate the SKN-1/Nrf2 Antioxidant Response.

In conclusion, we identified PEK-1 as a mediator of hormesis in the mev-1 mutant of C. elegans which is induced by simultaneous inhibition of XBP-1 and its splicing activator IRE-1 and mediated through activation of the proteasome.
Title: PEK-1 is crucial for hormesis induced by inhibition of the IRE-1/XBP-1 pathway in the Caenorhabditis elegans mev-1 mutant.

The following ire-1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ire-1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa337512	NM_001267206.2 Latest version!	Caenorhabditis elegans Serine/threonine-protein kinase (ire-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OCa338079	NM_001267207.2 Latest version!	Caenorhabditis elegans Serine/threonine-protein kinase (ire-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
OCa337512	NM_001267206.1	Caenorhabditis elegans Serine/threonine-protein kinase (ire-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OCa338079	NM_001267207.1	Caenorhabditis elegans Serine/threonine-protein kinase (ire-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ire-1 ( NM_001267206.2 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001254135.1 Caenorhabditis elegans Serine/threonine-protein kinase (ire-1), mRNA.

Price & Availability↑

CloneID	OCa337512
Clone ID Related Accession (Same CDS sequence)	NM_001267206.2 , NM_001267206.1
Accession Version	NM_001267206.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2904bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Serine/threonine-protein kinase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003280). On Sep 4, 2019 this sequence version replaced NM_001267206.1.

ATGAGAGCAA CATTTCATCT ATTCACATTT ATTTTTTTGC TGCTCTTCTC CTCTGTAATT 
TGTATTAGCA CACCTGGCTT CCGGAATGAT CACGAATCAA TAGGAGACGA CGAAGAGAAA 
ACATCTTCAA CTATACTTGT TTCGACAATT GATGGACGTT TGAGAGCTTT GGATTCAGAG 
ACGGGAGAAA TAAAATGGAC ATTGCAAGAA GAGCCTGTTT TACGATCTCC ATCTGCTGTC 
AAACAGGGAT TTACATTTCT TCCGAATCCG CTCGATGGAT CACTTTATGT TTTGAAAAAC 
AGTTCTTTGA AAAAACTTCC ATTCAATATT CCACAATTAG TTCATGCATC ACCTTGCAAA 
GGAAATGATG GAATTTTATA CGCAGGAAGT AAGAAAGATG TTTGGTTTGG AATTGATCCA 
AAAACAGGCT TAAAGGTGGA AACTCTATCA TCAGCGTCAG CGGATAGAAT CTGCCCTGCA 
AATCAAAAAC AGACAATTTT TTTGGGTCGA ACGGAATACA GGGTCTCTAT GTTTGATGAA 
AAGAATCGTG GAAAGACCTG GAATGCAACA TTCAATGATT ATTCTGCCCA TTTGTTGCCT 
GAAGTGAATA CGTGGCCATT TAAGCATTAT GCATCGTCGT CGCATGGATA TATACTGACT 
TTCGATAGAG AAACTGGAGA AATGAGATGG GAGCAAGATC TGAAACAACC TGTAGTTGCA 
CTTTATCTTC TTCGAGATGA TGGATTGCAC AAATTACCAT TTGAAGTGAT GGGAAAAGAG 
ACAATGGAAA ATGTAGCAAA GAATATCTTC ACCGTGGATC AATGGCCAAC AGTTTTGGGT 
GTCAATGCTG CAGATCCTCA AACAACATCC CTCACGAATC AATTCTTCCC AGCTTTATTC 
GTTGGAGAAT CTTCATTTGG ACTTTATGCT ATCGAAGCTC TAGTCGATCA CCAAACCATT 
ACATATTCTC CAAAACTTCT TGGTCCACCA CTTCTAGAAG GTCCAGCTCC AATTGCACTT 
ACAGAAATGG AAAAAGAAGA GTACTTGCCA CCACGGAGAC CAATAATTCG AAATATTCCT 
CCATCAATTA CTCATAAAAC TTCGGATGGA GAATACTTAT TATTAGGATA TCATGATCGA 
CCAATGATGA CGATGGCAAC GATTATTCCG ACAAGATATC CAGTTCCTGG ACCTCATAAG 
GCAATTGGAT CTACAATTGA ACGGCCTCCA CCACAACTTC TGGGACCTGT TGAGCCACAG 
AAGCATGAGG ACACATCTTT CATTCTGCTT CTACTCAATA ATCATCCTAT TCCATTCTAT 
GCTACACTGG TTACGATGTT TGCATTACTA TTAACTGTAA TTTGGCAATG TGGAAGACAA 
TGGGATCAAC AGAAGTCGAC GTCAAGAATG GATTCCTTCG AAATTGTAAA CAATCCCGGA 
GAATCTAGAA GTGCTCAGAC ATCAAAACAA TCGAATCGTG GAAGCTTTGG ATGGGCGAAT 
AGAAAAATTG AAATTCCCGA GGGTTGGATG GCAGTTGGTA GCAAGTTGAT GTATAGTCCA 
TCAGATATTC TTGGAACAGG ATGTGAAGGA ACTGTGGTTT ATAGAGGAAC TTTTGATGGA 
AGAGAAGTTG CTGTAAAGAG AGTAGTTTCA GAATTCGTGA AGTTTGCTCA CAGAGAAGCG 
GATTTATTAC GAGAAAGTGA TACACATCCA CACGTTATCA GATACTTTTG TATGGAATCA 
GACAGTCAAT TTCGATATTT GGCACTCGAA CTTTGCATAG CTTCTTTGAA TGATTATGTT 
GAGCAGAAAG AGGTTCAACA AAATGTTACA ATTGCTCTAC GAGACATTAT GAAACAGGCA 
ACCGATGGAT TGGCACATCT TCATGCTTCA AAAATTGTGC ATCGAGATAT GAAGCCACAA 
AATGTTCTAA TTACAATGGC TAGTCAGCGA GGAGAAATGA GAGCAGTCAT CTCAGATTTT 
GGTCTTTGTA AAAGGGTTCA ACCTGGAAAG AATTCAATTT CAAGAGGTAT TGCGTCTGGA 
TTAGCAGGAA CCGATGGCTG GATTGCTCCA GAAGTTCTTA TTTCTGCAAG TACTTCTTAT 
CCAGTTGATA TCTTCTCACT TGGATGTATT TTCTATTATG TGTTAACCTC GGGTACTCAT 
CCTTTTGGAA AATCATTGCA CAGACAAGCA AATATTGTAA ATGGAGAATA CACATTGAAT 
AAATTGGCAG ATTTGGATGA TTGGTCGTTG GCTGATGACT TGATCTCTTC AATGCTGAAC 
GTGGAGCCAT TGCACCGATT GACCGCTGAT GCCGTCTTGA ATCATCCATT TTTCTGGACA 
TCTGAAAAGC GATTAGCATA TTTTTCGGAT GTATCTGATC GTGTAGAGAA AGAAGAAGAC 
AATAGTCCAG TTGTGAGAAG AATAGAAACA GATGCTCGAA TAGTTGTATG CGGTGGATGG 
AGGGAGAAGA TTTGTGATGC TTTGAAGGAA GATTTAAGAA AGTTCAGAAC ATACAAATCA 
TTTTCAGTTC GAGATTTGCT AAGAGCAATG CGTAACAAGA AGCATCACTA TAGAGAATTA 
CCAGAAGATG TCAGACAATC TCTTGGAGAC ATTCCAGATC AGTTCTTGCA TTATTTTACA 
TCACGCTTCC CTCGGCTATT ACTTCATGTT TACAAAGCAA CCGAGTATTG TTCCGGAGAG 
GCTGTCTTCA AAAGATACTA TTCCGATGAT GTTCGTGCTC GAATGTATCC AATTGTGGAA 
GAAGAAGAGA GAGTAAGAAA GAAGATTAAA GAAGAAATGG CAAATGAGGT GTGGGCACGA 
GCACCAAAAC CTGTCGAGCA ACGAACTCCT CTAAAATTGG ATAAGAGGAA TATCAAAAAG 
AAATCAAATC CGAACACCGA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001254135.1
CDS	71..2974
Translation	MRATFHLFTFIFLLLFSSVICISTPGFRNDHESIGDDEEKTSSTILVSTIDGRLRALDSETGEIKWTLQEEPVLRSPSAVKQGFTFLPNPLDGSLYVLKNSSLKKLPFNIPQLVHASPCKGNDGILYAGSKKDVWFGIDPKTGLKVETLSSASADRICPANQKQTIFLGRTEYRVSMFDEKNRGKTWNATFNDYSAHLLPEVNTWPFKHYASSSHGYILTFDRETGEMRWEQDLKQPVVALYLLRDDGLHKLPFEVMGKETMENVAKNIFTVDQWPTVLGVNAADPQTTSLTNQFFPALFVGESSFGLYAIEALVDHQTITYSPKLLGPPLLEGPAPIALTEMEKEEYLPPRRPIIRNIPPSITHKTSDGEYLLLGYHDRPMMTMATIIPTRYPVPGPHKAIGSTIERPPPQLLGPVEPQKHEDTSFILLLLNNHPIPFYATLVTMFALLLTVIWQCGRQWDQQKSTSRMDSFEIVNNPGESRSAQTSKQSNRGSFGWANRKIEIPEGWMAVGSKLMYSPSDILGTGCEGTVVYRGTFDGREVAVKRVVSEFVKFAHREADLLRESDTHPHVIRYFCMESDSQFRYLALELCIASLNDYVEQKEVQQNVTIALRDIMKQATDGLAHLHASKIVHRDMKPQNVLITMASQRGEMRAVISDFGLCKRVQPGKNSISRGIASGLAGTDGWIAPEVLISASTSYPVDIFSLGCIFYYVLTSGTHPFGKSLHRQANIVNGEYTLNKLADLDDWSLADDLISSMLNVEPLHRLTADAVLNHPFFWTSEKRLAYFSDVSDRVEKEEDNSPVVRRIETDARIVVCGGWREKICDALKEDLRKFRTYKSFSVRDLLRAMRNKKHHYRELPEDVRQSLGDIPDQFLHYFTSRFPRLLLHVYKATEYCSGEAVFKRYYSDDVRARMYPIVEEEERVRKKIKEEMANEVWARAPKPVEQRTPLKLDKRNIKKKSNPNTD

Target ORF information:

RefSeq Version	NM_001267206.2
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Serine/threonine-protein kinase (ire-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001267206.2

ORF Insert Sequence:

ATGAGAGCAA CATTTCATCT ATTCACATTT ATTTTTTTGC TGCTCTTCTC CTCTGTAATT 
TGTATTAGCA CACCTGGCTT CCGGAATGAT CACGAATCAA TAGGAGACGA CGAAGAGAAA 
ACATCTTCAA CTATACTTGT TTCGACAATT GATGGACGTT TGAGAGCTTT GGATTCAGAG 
ACGGGAGAAA TAAAATGGAC ATTGCAAGAA GAGCCTGTTT TACGATCTCC ATCTGCTGTC 
AAACAGGGAT TTACATTTCT TCCGAATCCG CTCGATGGAT CACTTTATGT TTTGAAAAAC 
AGTTCTTTGA AAAAACTTCC ATTCAATATT CCACAATTAG TTCATGCATC ACCTTGCAAA 
GGAAATGATG GAATTTTATA CGCAGGAAGT AAGAAAGATG TTTGGTTTGG AATTGATCCA 
AAAACAGGCT TAAAGGTGGA AACTCTATCA TCAGCGTCAG CGGATAGAAT CTGCCCTGCA 
AATCAAAAAC AGACAATTTT TTTGGGTCGA ACGGAATACA GGGTCTCTAT GTTTGATGAA 
AAGAATCGTG GAAAGACCTG GAATGCAACA TTCAATGATT ATTCTGCCCA TTTGTTGCCT 
GAAGTGAATA CGTGGCCATT TAAGCATTAT GCATCGTCGT CGCATGGATA TATACTGACT 
TTCGATAGAG AAACTGGAGA AATGAGATGG GAGCAAGATC TGAAACAACC TGTAGTTGCA 
CTTTATCTTC TTCGAGATGA TGGATTGCAC AAATTACCAT TTGAAGTGAT GGGAAAAGAG 
ACAATGGAAA ATGTAGCAAA GAATATCTTC ACCGTGGATC AATGGCCAAC AGTTTTGGGT 
GTCAATGCTG CAGATCCTCA AACAACATCC CTCACGAATC AATTCTTCCC AGCTTTATTC 
GTTGGAGAAT CTTCATTTGG ACTTTATGCT ATCGAAGCTC TAGTCGATCA CCAAACCATT 
ACATATTCTC CAAAACTTCT TGGTCCACCA CTTCTAGAAG GTCCAGCTCC AATTGCACTT 
ACAGAAATGG AAAAAGAAGA GTACTTGCCA CCACGGAGAC CAATAATTCG AAATATTCCT 
CCATCAATTA CTCATAAAAC TTCGGATGGA GAATACTTAT TATTAGGATA TCATGATCGA 
CCAATGATGA CGATGGCAAC GATTATTCCG ACAAGATATC CAGTTCCTGG ACCTCATAAG 
GCAATTGGAT CTACAATTGA ACGGCCTCCA CCACAACTTC TGGGACCTGT TGAGCCACAG 
AAGCATGAGG ACACATCTTT CATTCTGCTT CTACTCAATA ATCATCCTAT TCCATTCTAT 
GCTACACTGG TTACGATGTT TGCATTACTA TTAACTGTAA TTTGGCAATG TGGAAGACAA 
TGGGATCAAC AGAAGTCGAC GTCAAGAATG GATTCCTTCG AAATTGTAAA CAATCCCGGA 
GAATCTAGAA GTGCTCAGAC ATCAAAACAA TCGAATCGTG GAAGCTTTGG ATGGGCGAAT 
AGAAAAATTG AAATTCCCGA GGGTTGGATG GCAGTTGGTA GCAAGTTGAT GTATAGTCCA 
TCAGATATTC TTGGAACAGG ATGTGAAGGA ACTGTGGTTT ATAGAGGAAC TTTTGATGGA 
AGAGAAGTTG CTGTAAAGAG AGTAGTTTCA GAATTCGTGA AGTTTGCTCA CAGAGAAGCG 
GATTTATTAC GAGAAAGTGA TACACATCCA CACGTTATCA GATACTTTTG TATGGAATCA 
GACAGTCAAT TTCGATATTT GGCACTCGAA CTTTGCATAG CTTCTTTGAA TGATTATGTT 
GAGCAGAAAG AGGTTCAACA AAATGTTACA ATTGCTCTAC GAGACATTAT GAAACAGGCA 
ACCGATGGAT TGGCACATCT TCATGCTTCA AAAATTGTGC ATCGAGATAT GAAGCCACAA 
AATGTTCTAA TTACAATGGC TAGTCAGCGA GGAGAAATGA GAGCAGTCAT CTCAGATTTT 
GGTCTTTGTA AAAGGGTTCA ACCTGGAAAG AATTCAATTT CAAGAGGTAT TGCGTCTGGA 
TTAGCAGGAA CCGATGGCTG GATTGCTCCA GAAGTTCTTA TTTCTGCAAG TACTTCTTAT 
CCAGTTGATA TCTTCTCACT TGGATGTATT TTCTATTATG TGTTAACCTC GGGTACTCAT 
CCTTTTGGAA AATCATTGCA CAGACAAGCA AATATTGTAA ATGGAGAATA CACATTGAAT 
AAATTGGCAG ATTTGGATGA TTGGTCGTTG GCTGATGACT TGATCTCTTC AATGCTGAAC 
GTGGAGCCAT TGCACCGATT GACCGCTGAT GCCGTCTTGA ATCATCCATT TTTCTGGACA 
TCTGAAAAGC GATTAGCATA TTTTTCGGAT GTATCTGATC GTGTAGAGAA AGAAGAAGAC 
AATAGTCCAG TTGTGAGAAG AATAGAAACA GATGCTCGAA TAGTTGTATG CGGTGGATGG 
AGGGAGAAGA TTTGTGATGC TTTGAAGGAA GATTTAAGAA AGTTCAGAAC ATACAAATCA 
TTTTCAGTTC GAGATTTGCT AAGAGCAATG CGTAACAAGA AGCATCACTA TAGAGAATTA 
CCAGAAGATG TCAGACAATC TCTTGGAGAC ATTCCAGATC AGTTCTTGCA TTATTTTACA 
TCACGCTTCC CTCGGCTATT ACTTCATGTT TACAAAGCAA CCGAGTATTG TTCCGGAGAG 
GCTGTCTTCA AAAGATACTA TTCCGATGAT GTTCGTGCTC GAATGTATCC AATTGTGGAA 
GAAGAAGAGA GAGTAAGAAA GAAGATTAAA GAAGAAATGG CAAATGAGGT GTGGGCACGA 
GCACCAAAAC CTGTCGAGCA ACGAACTCCT CTAAAATTGG ATAAGAGGAA TATCAAAAAG 
AAATCAAATC CGAACACCGA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ire-1 ( NM_001267206.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001254135.1 Caenorhabditis elegans Serine/threonine-protein kinase (ire-1), partial mRNA.

Price & Availability↑

CloneID	OCa337512
Clone ID Related Accession (Same CDS sequence)	NM_001267206.2 , NM_001267206.1
Accession Version	NM_001267206.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2904bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Serine/threonine-protein kinase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003280). COMPLETENESS: incomplete on both ends.

ATGAGAGCAA CATTTCATCT ATTCACATTT ATTTTTTTGC TGCTCTTCTC CTCTGTAATT 
TGTATTAGCA CACCTGGCTT CCGGAATGAT CACGAATCAA TAGGAGACGA CGAAGAGAAA 
ACATCTTCAA CTATACTTGT TTCGACAATT GATGGACGTT TGAGAGCTTT GGATTCAGAG 
ACGGGAGAAA TAAAATGGAC ATTGCAAGAA GAGCCTGTTT TACGATCTCC ATCTGCTGTC 
AAACAGGGAT TTACATTTCT TCCGAATCCG CTCGATGGAT CACTTTATGT TTTGAAAAAC 
AGTTCTTTGA AAAAACTTCC ATTCAATATT CCACAATTAG TTCATGCATC ACCTTGCAAA 
GGAAATGATG GAATTTTATA CGCAGGAAGT AAGAAAGATG TTTGGTTTGG AATTGATCCA 
AAAACAGGCT TAAAGGTGGA AACTCTATCA TCAGCGTCAG CGGATAGAAT CTGCCCTGCA 
AATCAAAAAC AGACAATTTT TTTGGGTCGA ACGGAATACA GGGTCTCTAT GTTTGATGAA 
AAGAATCGTG GAAAGACCTG GAATGCAACA TTCAATGATT ATTCTGCCCA TTTGTTGCCT 
GAAGTGAATA CGTGGCCATT TAAGCATTAT GCATCGTCGT CGCATGGATA TATACTGACT 
TTCGATAGAG AAACTGGAGA AATGAGATGG GAGCAAGATC TGAAACAACC TGTAGTTGCA 
CTTTATCTTC TTCGAGATGA TGGATTGCAC AAATTACCAT TTGAAGTGAT GGGAAAAGAG 
ACAATGGAAA ATGTAGCAAA GAATATCTTC ACCGTGGATC AATGGCCAAC AGTTTTGGGT 
GTCAATGCTG CAGATCCTCA AACAACATCC CTCACGAATC AATTCTTCCC AGCTTTATTC 
GTTGGAGAAT CTTCATTTGG ACTTTATGCT ATCGAAGCTC TAGTCGATCA CCAAACCATT 
ACATATTCTC CAAAACTTCT TGGTCCACCA CTTCTAGAAG GTCCAGCTCC AATTGCACTT 
ACAGAAATGG AAAAAGAAGA GTACTTGCCA CCACGGAGAC CAATAATTCG AAATATTCCT 
CCATCAATTA CTCATAAAAC TTCGGATGGA GAATACTTAT TATTAGGATA TCATGATCGA 
CCAATGATGA CGATGGCAAC GATTATTCCG ACAAGATATC CAGTTCCTGG ACCTCATAAG 
GCAATTGGAT CTACAATTGA ACGGCCTCCA CCACAACTTC TGGGACCTGT TGAGCCACAG 
AAGCATGAGG ACACATCTTT CATTCTGCTT CTACTCAATA ATCATCCTAT TCCATTCTAT 
GCTACACTGG TTACGATGTT TGCATTACTA TTAACTGTAA TTTGGCAATG TGGAAGACAA 
TGGGATCAAC AGAAGTCGAC GTCAAGAATG GATTCCTTCG AAATTGTAAA CAATCCCGGA 
GAATCTAGAA GTGCTCAGAC ATCAAAACAA TCGAATCGTG GAAGCTTTGG ATGGGCGAAT 
AGAAAAATTG AAATTCCCGA GGGTTGGATG GCAGTTGGTA GCAAGTTGAT GTATAGTCCA 
TCAGATATTC TTGGAACAGG ATGTGAAGGA ACTGTGGTTT ATAGAGGAAC TTTTGATGGA 
AGAGAAGTTG CTGTAAAGAG AGTAGTTTCA GAATTCGTGA AGTTTGCTCA CAGAGAAGCG 
GATTTATTAC GAGAAAGTGA TACACATCCA CACGTTATCA GATACTTTTG TATGGAATCA 
GACAGTCAAT TTCGATATTT GGCACTCGAA CTTTGCATAG CTTCTTTGAA TGATTATGTT 
GAGCAGAAAG AGGTTCAACA AAATGTTACA ATTGCTCTAC GAGACATTAT GAAACAGGCA 
ACCGATGGAT TGGCACATCT TCATGCTTCA AAAATTGTGC ATCGAGATAT GAAGCCACAA 
AATGTTCTAA TTACAATGGC TAGTCAGCGA GGAGAAATGA GAGCAGTCAT CTCAGATTTT 
GGTCTTTGTA AAAGGGTTCA ACCTGGAAAG AATTCAATTT CAAGAGGTAT TGCGTCTGGA 
TTAGCAGGAA CCGATGGCTG GATTGCTCCA GAAGTTCTTA TTTCTGCAAG TACTTCTTAT 
CCAGTTGATA TCTTCTCACT TGGATGTATT TTCTATTATG TGTTAACCTC GGGTACTCAT 
CCTTTTGGAA AATCATTGCA CAGACAAGCA AATATTGTAA ATGGAGAATA CACATTGAAT 
AAATTGGCAG ATTTGGATGA TTGGTCGTTG GCTGATGACT TGATCTCTTC AATGCTGAAC 
GTGGAGCCAT TGCACCGATT GACCGCTGAT GCCGTCTTGA ATCATCCATT TTTCTGGACA 
TCTGAAAAGC GATTAGCATA TTTTTCGGAT GTATCTGATC GTGTAGAGAA AGAAGAAGAC 
AATAGTCCAG TTGTGAGAAG AATAGAAACA GATGCTCGAA TAGTTGTATG CGGTGGATGG 
AGGGAGAAGA TTTGTGATGC TTTGAAGGAA GATTTAAGAA AGTTCAGAAC ATACAAATCA 
TTTTCAGTTC GAGATTTGCT AAGAGCAATG CGTAACAAGA AGCATCACTA TAGAGAATTA 
CCAGAAGATG TCAGACAATC TCTTGGAGAC ATTCCAGATC AGTTCTTGCA TTATTTTACA 
TCACGCTTCC CTCGGCTATT ACTTCATGTT TACAAAGCAA CCGAGTATTG TTCCGGAGAG 
GCTGTCTTCA AAAGATACTA TTCCGATGAT GTTCGTGCTC GAATGTATCC AATTGTGGAA 
GAAGAAGAGA GAGTAAGAAA GAAGATTAAA GAAGAAATGG CAAATGAGGT GTGGGCACGA 
GCACCAAAAC CTGTCGAGCA ACGAACTCCT CTAAAATTGG ATAAGAGGAA TATCAAAAAG 
AAATCAAATC CGAACACCGA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001254135.1
CDS	1..2904
Translation	MRATFHLFTFIFLLLFSSVICISTPGFRNDHESIGDDEEKTSSTILVSTIDGRLRALDSETGEIKWTLQEEPVLRSPSAVKQGFTFLPNPLDGSLYVLKNSSLKKLPFNIPQLVHASPCKGNDGILYAGSKKDVWFGIDPKTGLKVETLSSASADRICPANQKQTIFLGRTEYRVSMFDEKNRGKTWNATFNDYSAHLLPEVNTWPFKHYASSSHGYILTFDRETGEMRWEQDLKQPVVALYLLRDDGLHKLPFEVMGKETMENVAKNIFTVDQWPTVLGVNAADPQTTSLTNQFFPALFVGESSFGLYAIEALVDHQTITYSPKLLGPPLLEGPAPIALTEMEKEEYLPPRRPIIRNIPPSITHKTSDGEYLLLGYHDRPMMTMATIIPTRYPVPGPHKAIGSTIERPPPQLLGPVEPQKHEDTSFILLLLNNHPIPFYATLVTMFALLLTVIWQCGRQWDQQKSTSRMDSFEIVNNPGESRSAQTSKQSNRGSFGWANRKIEIPEGWMAVGSKLMYSPSDILGTGCEGTVVYRGTFDGREVAVKRVVSEFVKFAHREADLLRESDTHPHVIRYFCMESDSQFRYLALELCIASLNDYVEQKEVQQNVTIALRDIMKQATDGLAHLHASKIVHRDMKPQNVLITMASQRGEMRAVISDFGLCKRVQPGKNSISRGIASGLAGTDGWIAPEVLISASTSYPVDIFSLGCIFYYVLTSGTHPFGKSLHRQANIVNGEYTLNKLADLDDWSLADDLISSMLNVEPLHRLTADAVLNHPFFWTSEKRLAYFSDVSDRVEKEEDNSPVVRRIETDARIVVCGGWREKICDALKEDLRKFRTYKSFSVRDLLRAMRNKKHHYRELPEDVRQSLGDIPDQFLHYFTSRFPRLLLHVYKATEYCSGEAVFKRYYSDDVRARMYPIVEEEERVRKKIKEEMANEVWARAPKPVEQRTPLKLDKRNIKKKSNPNTD

Target ORF information:

RefSeq Version	NM_001267206.1
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Serine/threonine-protein kinase (ire-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001267206.1

ORF Insert Sequence:

ATGAGAGCAA CATTTCATCT ATTCACATTT ATTTTTTTGC TGCTCTTCTC CTCTGTAATT 
TGTATTAGCA CACCTGGCTT CCGGAATGAT CACGAATCAA TAGGAGACGA CGAAGAGAAA 
ACATCTTCAA CTATACTTGT TTCGACAATT GATGGACGTT TGAGAGCTTT GGATTCAGAG 
ACGGGAGAAA TAAAATGGAC ATTGCAAGAA GAGCCTGTTT TACGATCTCC ATCTGCTGTC 
AAACAGGGAT TTACATTTCT TCCGAATCCG CTCGATGGAT CACTTTATGT TTTGAAAAAC 
AGTTCTTTGA AAAAACTTCC ATTCAATATT CCACAATTAG TTCATGCATC ACCTTGCAAA 
GGAAATGATG GAATTTTATA CGCAGGAAGT AAGAAAGATG TTTGGTTTGG AATTGATCCA 
AAAACAGGCT TAAAGGTGGA AACTCTATCA TCAGCGTCAG CGGATAGAAT CTGCCCTGCA 
AATCAAAAAC AGACAATTTT TTTGGGTCGA ACGGAATACA GGGTCTCTAT GTTTGATGAA 
AAGAATCGTG GAAAGACCTG GAATGCAACA TTCAATGATT ATTCTGCCCA TTTGTTGCCT 
GAAGTGAATA CGTGGCCATT TAAGCATTAT GCATCGTCGT CGCATGGATA TATACTGACT 
TTCGATAGAG AAACTGGAGA AATGAGATGG GAGCAAGATC TGAAACAACC TGTAGTTGCA 
CTTTATCTTC TTCGAGATGA TGGATTGCAC AAATTACCAT TTGAAGTGAT GGGAAAAGAG 
ACAATGGAAA ATGTAGCAAA GAATATCTTC ACCGTGGATC AATGGCCAAC AGTTTTGGGT 
GTCAATGCTG CAGATCCTCA AACAACATCC CTCACGAATC AATTCTTCCC AGCTTTATTC 
GTTGGAGAAT CTTCATTTGG ACTTTATGCT ATCGAAGCTC TAGTCGATCA CCAAACCATT 
ACATATTCTC CAAAACTTCT TGGTCCACCA CTTCTAGAAG GTCCAGCTCC AATTGCACTT 
ACAGAAATGG AAAAAGAAGA GTACTTGCCA CCACGGAGAC CAATAATTCG AAATATTCCT 
CCATCAATTA CTCATAAAAC TTCGGATGGA GAATACTTAT TATTAGGATA TCATGATCGA 
CCAATGATGA CGATGGCAAC GATTATTCCG ACAAGATATC CAGTTCCTGG ACCTCATAAG 
GCAATTGGAT CTACAATTGA ACGGCCTCCA CCACAACTTC TGGGACCTGT TGAGCCACAG 
AAGCATGAGG ACACATCTTT CATTCTGCTT CTACTCAATA ATCATCCTAT TCCATTCTAT 
GCTACACTGG TTACGATGTT TGCATTACTA TTAACTGTAA TTTGGCAATG TGGAAGACAA 
TGGGATCAAC AGAAGTCGAC GTCAAGAATG GATTCCTTCG AAATTGTAAA CAATCCCGGA 
GAATCTAGAA GTGCTCAGAC ATCAAAACAA TCGAATCGTG GAAGCTTTGG ATGGGCGAAT 
AGAAAAATTG AAATTCCCGA GGGTTGGATG GCAGTTGGTA GCAAGTTGAT GTATAGTCCA 
TCAGATATTC TTGGAACAGG ATGTGAAGGA ACTGTGGTTT ATAGAGGAAC TTTTGATGGA 
AGAGAAGTTG CTGTAAAGAG AGTAGTTTCA GAATTCGTGA AGTTTGCTCA CAGAGAAGCG 
GATTTATTAC GAGAAAGTGA TACACATCCA CACGTTATCA GATACTTTTG TATGGAATCA 
GACAGTCAAT TTCGATATTT GGCACTCGAA CTTTGCATAG CTTCTTTGAA TGATTATGTT 
GAGCAGAAAG AGGTTCAACA AAATGTTACA ATTGCTCTAC GAGACATTAT GAAACAGGCA 
ACCGATGGAT TGGCACATCT TCATGCTTCA AAAATTGTGC ATCGAGATAT GAAGCCACAA 
AATGTTCTAA TTACAATGGC TAGTCAGCGA GGAGAAATGA GAGCAGTCAT CTCAGATTTT 
GGTCTTTGTA AAAGGGTTCA ACCTGGAAAG AATTCAATTT CAAGAGGTAT TGCGTCTGGA 
TTAGCAGGAA CCGATGGCTG GATTGCTCCA GAAGTTCTTA TTTCTGCAAG TACTTCTTAT 
CCAGTTGATA TCTTCTCACT TGGATGTATT TTCTATTATG TGTTAACCTC GGGTACTCAT 
CCTTTTGGAA AATCATTGCA CAGACAAGCA AATATTGTAA ATGGAGAATA CACATTGAAT 
AAATTGGCAG ATTTGGATGA TTGGTCGTTG GCTGATGACT TGATCTCTTC AATGCTGAAC 
GTGGAGCCAT TGCACCGATT GACCGCTGAT GCCGTCTTGA ATCATCCATT TTTCTGGACA 
TCTGAAAAGC GATTAGCATA TTTTTCGGAT GTATCTGATC GTGTAGAGAA AGAAGAAGAC 
AATAGTCCAG TTGTGAGAAG AATAGAAACA GATGCTCGAA TAGTTGTATG CGGTGGATGG 
AGGGAGAAGA TTTGTGATGC TTTGAAGGAA GATTTAAGAA AGTTCAGAAC ATACAAATCA 
TTTTCAGTTC GAGATTTGCT AAGAGCAATG CGTAACAAGA AGCATCACTA TAGAGAATTA 
CCAGAAGATG TCAGACAATC TCTTGGAGAC ATTCCAGATC AGTTCTTGCA TTATTTTACA 
TCACGCTTCC CTCGGCTATT ACTTCATGTT TACAAAGCAA CCGAGTATTG TTCCGGAGAG 
GCTGTCTTCA AAAGATACTA TTCCGATGAT GTTCGTGCTC GAATGTATCC AATTGTGGAA 
GAAGAAGAGA GAGTAAGAAA GAAGATTAAA GAAGAAATGG CAAATGAGGT GTGGGCACGA 
GCACCAAAAC CTGTCGAGCA ACGAACTCCT CTAAAATTGG ATAAGAGGAA TATCAAAAAG 
AAATCAAATC CGAACACCGA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ire-1 ( NM_001267207.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001254136.1 Caenorhabditis elegans Serine/threonine-protein kinase (ire-1), partial mRNA.

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CloneID	OCa338079
Clone ID Related Accession (Same CDS sequence)	NM_001267207.1 , NM_001267207.2
Accession Version	NM_001267207.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1878bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Serine/threonine-protein kinase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003280). COMPLETENESS: incomplete on both ends.

ATGGAAAAAG AAGAGTACTT GCCACCACGG AGACCAATAA TTCGAAATAT TCCTCCATCA 
ATTACTCATA AAACTTCGGA TGGAGAATAC TTATTATTAG GATATCATGA TCGACCAATG 
ATGACGATGG CAACGATTAT TCCGACAAGA TATCCAGTTC CTGGACCTCA TAAGGCAATT 
GGATCTACAA TTGAACGGCC TCCACCACAA CTTCTGGGAC CTGTTGAGCC ACAGAAGCAT 
GAGGACACAT CTTTCATTCT GCTTCTACTC AATAATCATC CTATTCCATT CTATGCTACA 
CTGGTTACGA TGTTTGCATT ACTATTAACT GTAATTTGGC AATGTGGAAG ACAATGGGAT 
CAACAGAAGT CGACGTCAAG AATGGATTCC TTCGAAATTG TAAACAATCC CGGAGAATCT 
AGAAGTGCTC AGACATCAAA ACAATCGAAT CGTGGAAGCT TTGGATGGGC GAATAGAAAA 
ATTGAAATTC CCGAGGGTTG GATGGCAGTT GGTAGCAAGT TGATGTATAG TCCATCAGAT 
ATTCTTGGAA CAGGATGTGA AGGAACTGTG GTTTATAGAG GAACTTTTGA TGGAAGAGAA 
GTTGCTGTAA AGAGAGTAGT TTCAGAATTC GTGAAGTTTG CTCACAGAGA AGCGGATTTA 
TTACGAGAAA GTGATACACA TCCACACGTT ATCAGATACT TTTGTATGGA ATCAGACAGT 
CAATTTCGAT ATTTGGCACT CGAACTTTGC ATAGCTTCTT TGAATGATTA TGTTGAGCAG 
AAAGAGGTTC AACAAAATGT TACAATTGCT CTACGAGACA TTATGAAACA GGCAACCGAT 
GGATTGGCAC ATCTTCATGC TTCAAAAATT GTGCATCGAG ATATGAAGCC ACAAAATGTT 
CTAATTACAA TGGCTAGTCA GCGAGGAGAA ATGAGAGCAG TCATCTCAGA TTTTGGTCTT 
TGTAAAAGGG TTCAACCTGG AAAGAATTCA ATTTCAAGAG GTATTGCGTC TGGATTAGCA 
GGAACCGATG GCTGGATTGC TCCAGAAGTT CTTATTTCTG CAAGTACTTC TTATCCAGTT 
GATATCTTCT CACTTGGATG TATTTTCTAT TATGTGTTAA CCTCGGGTAC TCATCCTTTT 
GGAAAATCAT TGCACAGACA AGCAAATATT GTAAATGGAG AATACACATT GAATAAATTG 
GCAGATTTGG ATGATTGGTC GTTGGCTGAT GACTTGATCT CTTCAATGCT GAACGTGGAG 
CCATTGCACC GATTGACCGC TGATGCCGTC TTGAATCATC CATTTTTCTG GACATCTGAA 
AAGCGATTAG CATATTTTTC GGATGTATCT GATCGTGTAG AGAAAGAAGA AGACAATAGT 
CCAGTTGTGA GAAGAATAGA AACAGATGCT CGAATAGTTG TATGCGGTGG ATGGAGGGAG 
AAGATTTGTG ATGCTTTGAA GGAAGATTTA AGAAAGTTCA GAACATACAA ATCATTTTCA 
GTTCGAGATT TGCTAAGAGC AATGCGTAAC AAGAAGCATC ACTATAGAGA ATTACCAGAA 
GATGTCAGAC AATCTCTTGG AGACATTCCA GATCAGTTCT TGCATTATTT TACATCACGC 
TTCCCTCGGC TATTACTTCA TGTTTACAAA GCAACCGAGT ATTGTTCCGG AGAGGCTGTC 
TTCAAAAGAT ACTATTCCGA TGATGTTCGT GCTCGAATGT ATCCAATTGT GGAAGAAGAA 
GAGAGAGTAA GAAAGAAGAT TAAAGAAGAA ATGGCAAATG AGGTGTGGGC ACGAGCACCA 
AAACCTGTCG AGCAACGAAC TCCTCTAAAA TTGGATAAGA GGAATATCAA AAAGAAATCA 
AATCCGAACA CCGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001254136.1
CDS	1..1878
Translation	MEKEEYLPPRRPIIRNIPPSITHKTSDGEYLLLGYHDRPMMTMATIIPTRYPVPGPHKAIGSTIERPPPQLLGPVEPQKHEDTSFILLLLNNHPIPFYATLVTMFALLLTVIWQCGRQWDQQKSTSRMDSFEIVNNPGESRSAQTSKQSNRGSFGWANRKIEIPEGWMAVGSKLMYSPSDILGTGCEGTVVYRGTFDGREVAVKRVVSEFVKFAHREADLLRESDTHPHVIRYFCMESDSQFRYLALELCIASLNDYVEQKEVQQNVTIALRDIMKQATDGLAHLHASKIVHRDMKPQNVLITMASQRGEMRAVISDFGLCKRVQPGKNSISRGIASGLAGTDGWIAPEVLISASTSYPVDIFSLGCIFYYVLTSGTHPFGKSLHRQANIVNGEYTLNKLADLDDWSLADDLISSMLNVEPLHRLTADAVLNHPFFWTSEKRLAYFSDVSDRVEKEEDNSPVVRRIETDARIVVCGGWREKICDALKEDLRKFRTYKSFSVRDLLRAMRNKKHHYRELPEDVRQSLGDIPDQFLHYFTSRFPRLLLHVYKATEYCSGEAVFKRYYSDDVRARMYPIVEEEERVRKKIKEEMANEVWARAPKPVEQRTPLKLDKRNIKKKSNPNTD

Target ORF information:

RefSeq Version	NM_001267207.1
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Serine/threonine-protein kinase (ire-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001267207.1

ORF Insert Sequence:

ATGGAAAAAG AAGAGTACTT GCCACCACGG AGACCAATAA TTCGAAATAT TCCTCCATCA 
ATTACTCATA AAACTTCGGA TGGAGAATAC TTATTATTAG GATATCATGA TCGACCAATG 
ATGACGATGG CAACGATTAT TCCGACAAGA TATCCAGTTC CTGGACCTCA TAAGGCAATT 
GGATCTACAA TTGAACGGCC TCCACCACAA CTTCTGGGAC CTGTTGAGCC ACAGAAGCAT 
GAGGACACAT CTTTCATTCT GCTTCTACTC AATAATCATC CTATTCCATT CTATGCTACA 
CTGGTTACGA TGTTTGCATT ACTATTAACT GTAATTTGGC AATGTGGAAG ACAATGGGAT 
CAACAGAAGT CGACGTCAAG AATGGATTCC TTCGAAATTG TAAACAATCC CGGAGAATCT 
AGAAGTGCTC AGACATCAAA ACAATCGAAT CGTGGAAGCT TTGGATGGGC GAATAGAAAA 
ATTGAAATTC CCGAGGGTTG GATGGCAGTT GGTAGCAAGT TGATGTATAG TCCATCAGAT 
ATTCTTGGAA CAGGATGTGA AGGAACTGTG GTTTATAGAG GAACTTTTGA TGGAAGAGAA 
GTTGCTGTAA AGAGAGTAGT TTCAGAATTC GTGAAGTTTG CTCACAGAGA AGCGGATTTA 
TTACGAGAAA GTGATACACA TCCACACGTT ATCAGATACT TTTGTATGGA ATCAGACAGT 
CAATTTCGAT ATTTGGCACT CGAACTTTGC ATAGCTTCTT TGAATGATTA TGTTGAGCAG 
AAAGAGGTTC AACAAAATGT TACAATTGCT CTACGAGACA TTATGAAACA GGCAACCGAT 
GGATTGGCAC ATCTTCATGC TTCAAAAATT GTGCATCGAG ATATGAAGCC ACAAAATGTT 
CTAATTACAA TGGCTAGTCA GCGAGGAGAA ATGAGAGCAG TCATCTCAGA TTTTGGTCTT 
TGTAAAAGGG TTCAACCTGG AAAGAATTCA ATTTCAAGAG GTATTGCGTC TGGATTAGCA 
GGAACCGATG GCTGGATTGC TCCAGAAGTT CTTATTTCTG CAAGTACTTC TTATCCAGTT 
GATATCTTCT CACTTGGATG TATTTTCTAT TATGTGTTAA CCTCGGGTAC TCATCCTTTT 
GGAAAATCAT TGCACAGACA AGCAAATATT GTAAATGGAG AATACACATT GAATAAATTG 
GCAGATTTGG ATGATTGGTC GTTGGCTGAT GACTTGATCT CTTCAATGCT GAACGTGGAG 
CCATTGCACC GATTGACCGC TGATGCCGTC TTGAATCATC CATTTTTCTG GACATCTGAA 
AAGCGATTAG CATATTTTTC GGATGTATCT GATCGTGTAG AGAAAGAAGA AGACAATAGT 
CCAGTTGTGA GAAGAATAGA AACAGATGCT CGAATAGTTG TATGCGGTGG ATGGAGGGAG 
AAGATTTGTG ATGCTTTGAA GGAAGATTTA AGAAAGTTCA GAACATACAA ATCATTTTCA 
GTTCGAGATT TGCTAAGAGC AATGCGTAAC AAGAAGCATC ACTATAGAGA ATTACCAGAA 
GATGTCAGAC AATCTCTTGG AGACATTCCA GATCAGTTCT TGCATTATTT TACATCACGC 
TTCCCTCGGC TATTACTTCA TGTTTACAAA GCAACCGAGT ATTGTTCCGG AGAGGCTGTC 
TTCAAAAGAT ACTATTCCGA TGATGTTCGT GCTCGAATGT ATCCAATTGT GGAAGAAGAA 
GAGAGAGTAA GAAAGAAGAT TAAAGAAGAA ATGGCAAATG AGGTGTGGGC ACGAGCACCA 
AAACCTGTCG AGCAACGAAC TCCTCTAAAA TTGGATAAGA GGAATATCAA AAAGAAATCA 
AATCCGAACA CCGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ire-1 ( NM_001267207.2 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001254136.1 Caenorhabditis elegans Serine/threonine-protein kinase (ire-1), partial mRNA.

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CloneID	OCa338079
Clone ID Related Accession (Same CDS sequence)	NM_001267207.1 , NM_001267207.2
Accession Version	NM_001267207.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1878bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Serine/threonine-protein kinase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003280). On Sep 4, 2019 this sequence version replaced NM_001267207.1. COMPLETENESS: incomplete on the 5' end.

ATGGAAAAAG AAGAGTACTT GCCACCACGG AGACCAATAA TTCGAAATAT TCCTCCATCA 
ATTACTCATA AAACTTCGGA TGGAGAATAC TTATTATTAG GATATCATGA TCGACCAATG 
ATGACGATGG CAACGATTAT TCCGACAAGA TATCCAGTTC CTGGACCTCA TAAGGCAATT 
GGATCTACAA TTGAACGGCC TCCACCACAA CTTCTGGGAC CTGTTGAGCC ACAGAAGCAT 
GAGGACACAT CTTTCATTCT GCTTCTACTC AATAATCATC CTATTCCATT CTATGCTACA 
CTGGTTACGA TGTTTGCATT ACTATTAACT GTAATTTGGC AATGTGGAAG ACAATGGGAT 
CAACAGAAGT CGACGTCAAG AATGGATTCC TTCGAAATTG TAAACAATCC CGGAGAATCT 
AGAAGTGCTC AGACATCAAA ACAATCGAAT CGTGGAAGCT TTGGATGGGC GAATAGAAAA 
ATTGAAATTC CCGAGGGTTG GATGGCAGTT GGTAGCAAGT TGATGTATAG TCCATCAGAT 
ATTCTTGGAA CAGGATGTGA AGGAACTGTG GTTTATAGAG GAACTTTTGA TGGAAGAGAA 
GTTGCTGTAA AGAGAGTAGT TTCAGAATTC GTGAAGTTTG CTCACAGAGA AGCGGATTTA 
TTACGAGAAA GTGATACACA TCCACACGTT ATCAGATACT TTTGTATGGA ATCAGACAGT 
CAATTTCGAT ATTTGGCACT CGAACTTTGC ATAGCTTCTT TGAATGATTA TGTTGAGCAG 
AAAGAGGTTC AACAAAATGT TACAATTGCT CTACGAGACA TTATGAAACA GGCAACCGAT 
GGATTGGCAC ATCTTCATGC TTCAAAAATT GTGCATCGAG ATATGAAGCC ACAAAATGTT 
CTAATTACAA TGGCTAGTCA GCGAGGAGAA ATGAGAGCAG TCATCTCAGA TTTTGGTCTT 
TGTAAAAGGG TTCAACCTGG AAAGAATTCA ATTTCAAGAG GTATTGCGTC TGGATTAGCA 
GGAACCGATG GCTGGATTGC TCCAGAAGTT CTTATTTCTG CAAGTACTTC TTATCCAGTT 
GATATCTTCT CACTTGGATG TATTTTCTAT TATGTGTTAA CCTCGGGTAC TCATCCTTTT 
GGAAAATCAT TGCACAGACA AGCAAATATT GTAAATGGAG AATACACATT GAATAAATTG 
GCAGATTTGG ATGATTGGTC GTTGGCTGAT GACTTGATCT CTTCAATGCT GAACGTGGAG 
CCATTGCACC GATTGACCGC TGATGCCGTC TTGAATCATC CATTTTTCTG GACATCTGAA 
AAGCGATTAG CATATTTTTC GGATGTATCT GATCGTGTAG AGAAAGAAGA AGACAATAGT 
CCAGTTGTGA GAAGAATAGA AACAGATGCT CGAATAGTTG TATGCGGTGG ATGGAGGGAG 
AAGATTTGTG ATGCTTTGAA GGAAGATTTA AGAAAGTTCA GAACATACAA ATCATTTTCA 
GTTCGAGATT TGCTAAGAGC AATGCGTAAC AAGAAGCATC ACTATAGAGA ATTACCAGAA 
GATGTCAGAC AATCTCTTGG AGACATTCCA GATCAGTTCT TGCATTATTT TACATCACGC 
TTCCCTCGGC TATTACTTCA TGTTTACAAA GCAACCGAGT ATTGTTCCGG AGAGGCTGTC 
TTCAAAAGAT ACTATTCCGA TGATGTTCGT GCTCGAATGT ATCCAATTGT GGAAGAAGAA 
GAGAGAGTAA GAAAGAAGAT TAAAGAAGAA ATGGCAAATG AGGTGTGGGC ACGAGCACCA 
AAACCTGTCG AGCAACGAAC TCCTCTAAAA TTGGATAAGA GGAATATCAA AAAGAAATCA 
AATCCGAACA CCGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001254136.1
CDS	1..1878
Translation	MEKEEYLPPRRPIIRNIPPSITHKTSDGEYLLLGYHDRPMMTMATIIPTRYPVPGPHKAIGSTIERPPPQLLGPVEPQKHEDTSFILLLLNNHPIPFYATLVTMFALLLTVIWQCGRQWDQQKSTSRMDSFEIVNNPGESRSAQTSKQSNRGSFGWANRKIEIPEGWMAVGSKLMYSPSDILGTGCEGTVVYRGTFDGREVAVKRVVSEFVKFAHREADLLRESDTHPHVIRYFCMESDSQFRYLALELCIASLNDYVEQKEVQQNVTIALRDIMKQATDGLAHLHASKIVHRDMKPQNVLITMASQRGEMRAVISDFGLCKRVQPGKNSISRGIASGLAGTDGWIAPEVLISASTSYPVDIFSLGCIFYYVLTSGTHPFGKSLHRQANIVNGEYTLNKLADLDDWSLADDLISSMLNVEPLHRLTADAVLNHPFFWTSEKRLAYFSDVSDRVEKEEDNSPVVRRIETDARIVVCGGWREKICDALKEDLRKFRTYKSFSVRDLLRAMRNKKHHYRELPEDVRQSLGDIPDQFLHYFTSRFPRLLLHVYKATEYCSGEAVFKRYYSDDVRARMYPIVEEEERVRKKIKEEMANEVWARAPKPVEQRTPLKLDKRNIKKKSNPNTD

Target ORF information:

RefSeq Version	NM_001267207.2
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Serine/threonine-protein kinase (ire-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001267207.2

ORF Insert Sequence:

ATGGAAAAAG AAGAGTACTT GCCACCACGG AGACCAATAA TTCGAAATAT TCCTCCATCA 
ATTACTCATA AAACTTCGGA TGGAGAATAC TTATTATTAG GATATCATGA TCGACCAATG 
ATGACGATGG CAACGATTAT TCCGACAAGA TATCCAGTTC CTGGACCTCA TAAGGCAATT 
GGATCTACAA TTGAACGGCC TCCACCACAA CTTCTGGGAC CTGTTGAGCC ACAGAAGCAT 
GAGGACACAT CTTTCATTCT GCTTCTACTC AATAATCATC CTATTCCATT CTATGCTACA 
CTGGTTACGA TGTTTGCATT ACTATTAACT GTAATTTGGC AATGTGGAAG ACAATGGGAT 
CAACAGAAGT CGACGTCAAG AATGGATTCC TTCGAAATTG TAAACAATCC CGGAGAATCT 
AGAAGTGCTC AGACATCAAA ACAATCGAAT CGTGGAAGCT TTGGATGGGC GAATAGAAAA 
ATTGAAATTC CCGAGGGTTG GATGGCAGTT GGTAGCAAGT TGATGTATAG TCCATCAGAT 
ATTCTTGGAA CAGGATGTGA AGGAACTGTG GTTTATAGAG GAACTTTTGA TGGAAGAGAA 
GTTGCTGTAA AGAGAGTAGT TTCAGAATTC GTGAAGTTTG CTCACAGAGA AGCGGATTTA 
TTACGAGAAA GTGATACACA TCCACACGTT ATCAGATACT TTTGTATGGA ATCAGACAGT 
CAATTTCGAT ATTTGGCACT CGAACTTTGC ATAGCTTCTT TGAATGATTA TGTTGAGCAG 
AAAGAGGTTC AACAAAATGT TACAATTGCT CTACGAGACA TTATGAAACA GGCAACCGAT 
GGATTGGCAC ATCTTCATGC TTCAAAAATT GTGCATCGAG ATATGAAGCC ACAAAATGTT 
CTAATTACAA TGGCTAGTCA GCGAGGAGAA ATGAGAGCAG TCATCTCAGA TTTTGGTCTT 
TGTAAAAGGG TTCAACCTGG AAAGAATTCA ATTTCAAGAG GTATTGCGTC TGGATTAGCA 
GGAACCGATG GCTGGATTGC TCCAGAAGTT CTTATTTCTG CAAGTACTTC TTATCCAGTT 
GATATCTTCT CACTTGGATG TATTTTCTAT TATGTGTTAA CCTCGGGTAC TCATCCTTTT 
GGAAAATCAT TGCACAGACA AGCAAATATT GTAAATGGAG AATACACATT GAATAAATTG 
GCAGATTTGG ATGATTGGTC GTTGGCTGAT GACTTGATCT CTTCAATGCT GAACGTGGAG 
CCATTGCACC GATTGACCGC TGATGCCGTC TTGAATCATC CATTTTTCTG GACATCTGAA 
AAGCGATTAG CATATTTTTC GGATGTATCT GATCGTGTAG AGAAAGAAGA AGACAATAGT 
CCAGTTGTGA GAAGAATAGA AACAGATGCT CGAATAGTTG TATGCGGTGG ATGGAGGGAG 
AAGATTTGTG ATGCTTTGAA GGAAGATTTA AGAAAGTTCA GAACATACAA ATCATTTTCA 
GTTCGAGATT TGCTAAGAGC AATGCGTAAC AAGAAGCATC ACTATAGAGA ATTACCAGAA 
GATGTCAGAC AATCTCTTGG AGACATTCCA GATCAGTTCT TGCATTATTT TACATCACGC 
TTCCCTCGGC TATTACTTCA TGTTTACAAA GCAACCGAGT ATTGTTCCGG AGAGGCTGTC 
TTCAAAAGAT ACTATTCCGA TGATGTTCGT GCTCGAATGT ATCCAATTGT GGAAGAAGAA 
GAGAGAGTAA GAAAGAAGAT TAAAGAAGAA ATGGCAAATG AGGTGTGGGC ACGAGCACCA 
AAACCTGTCG AGCAACGAAC TCCTCTAAAA TTGGATAAGA GGAATATCAA AAAGAAATCA 
AATCCGAACA CCGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.
Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

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ire-1 cDNA ORF clone, Caenorhabditis elegans

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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