ERN1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	ERN1
Entrez Gene ID	2081
Full Name	endoplasmic reticulum to nucleus signaling 1
Synonyms	IRE1,IRE1P,IRE1a,hIRE1p
General protein information	Preferred Names endoplasmic reticulum to nucleus signaling 1 Names serine/threonine-protein kinase/endoribonuclease IRE1 ER to nucleus signalling 1 inositol-requiring 1 inositol-requiring enzyme 1 inositol-requiring protein 1 ire1-alpha protein kinase/endoribonuclease
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 17 Map Location: 17q23.3
Summary	This gene encodes the transmembrane protein kinase inositol-requiring enzyme 1. The encoded protein contains two functional catalytic domains, a serine/threonine-protein kinase domain and an endoribonuclease domain. This protein functions as a sensor of unfolded proteins in the endoplasmic reticulum (ER) and triggers an intracellular signaling pathway termed the unfolded protein response (UPR). The UPR is an ER stress response that is conserved from yeast to mammals and activates genes involved in degrading misfolded proteins, regulating protein synthesis and activating molecular chaperones. This protein specifically mediates the splicing and activation of the stress response transcription factor X-box binding protein 1. [provided by RefSeq, Aug 2017].
Disorder MIM:	604033

mRNA and Protein(s)

mRNA	Protein	Name
NM_001433.4	NP_001424.3	serine/threonine-protein kinase/endoribonuclease IRE1 precursor
XM_017024348.1	XP_016879837.1	serine/threonine-protein kinase/endoribonuclease IRE1 isoform X2
NM_001433.5	NP_001424.3	serine/threonine-protein kinase/endoribonuclease IRE1 precursor
NM_001433.3	NP_001424.3	serine/threonine-protein kinase/endoribonuclease IRE1 precursor
XM_017024348.2	XP_016879837.1	serine/threonine-protein kinase/endoribonuclease IRE1 isoform X2
XM_017024347.1	XP_016879836.1	serine/threonine-protein kinase/endoribonuclease IRE1 isoform X1
XM_017024347.2	XP_016879836.1	serine/threonine-protein kinase/endoribonuclease IRE1 isoform X1

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Ern1	NP_001178855.1
	ERN1	XP_001109583.2
Canis lupus familiaris (dog)	ERN1	XP_853409.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004176	XP_562694.4
Mus musculus (house mouse)	Ern1	NP_076402.1
Xenopus tropicalis (tropical clawed frog)	ern2	NP_001072279.1
Homo sapiens (human)	ERN1	NP_001424.3
Pan troglodytes (chimpanzee)	ERN1	XP_511585.3
Danio rerio (zebrafish)	ern2	XP_001919350.3
Drosophila melanogaster (fruit fly)	Ire1	NP_001097839.1
Bos taurus (cattle)	ERN1	XP_002696144.1
Gallus gallus (chicken)	LOC417414	XP_004946240.1
Caenorhabditis elegans (roundworm)	ire-1	NP_001254135.1

Gene Ontology
Bibliography

Related articles in PubMed

A J-Protein Co-chaperone Recruits BiP to Monomerize IRE1 and Repress the Unfolded Protein Response.
Amin-Wetzel N, Saunders RA, Kamphuis MJ, Rato C, Preissler S, Harding HP, Ron D
Cell171(7)1625-1637.e13(2017 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Inhibition of IRE1 modifies hypoxic regulation of pentose-phosphate pathway genes expression in cultured glioma cells.
Title: Inhibition of IRE1 modifies hypoxic regulation of G6PD, GPI, TKT, TALDO1, PGLS and RPIA genes expression in U87 glioma cells.

The present study demonstrated that the inhibition of IRE1 in glioma cells affected the hypoxic regulation of the expression of down-stream genes in various directions, though hypoxic conditions did not abolish the effect of IRE1 inhibition on the expression of respective genes.
Title: Hypoxic regulation of MYBL1, MEST, TCF3, TCF8, GTF2B, GTF2F2 and SNAI2 genes expression in U87 glioma cells upon IRE1 inhibition.

The inhibition of IRE1 changes sensitivity of the expression of down-stream genes to glutamine deprivation in cultured glial tumor cells.
Title: Expression of IGFBP6, IGFBP7, NOV, CYR61, WISP1 and WISP2 genes in U87 glioma cells in glutamine deprivation condition.

Changes in the expression level of nuclear genes encoding mitochondrial proteins possibly reflect metabolic reprogramming of mitochondria by hypoxia and IRE1-mediated endoplasmic reticulum stress signaling and correlate with suppression of glioma cell proliferation under inhibition of the IRE1 enzyme function.
Title: Effect of hypoxia on the expression of nuclear genes encoding mitochondrial proteins in U87 glioma cells.

Inhibition of IRE1 modifies the hypoxic regulation of GADD34 family gene expression in cultured glioma cells.
Title: Inhibition of IRE1 modifies the hypoxic regulation of GADD family gene expressions in U87 glioma cells.

The following ERN1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ERN1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu25357	NM_001433.5 Latest version!	Homo sapiens endoplasmic reticulum to nucleus signaling 1 (ERN1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu86687	XM_017024348.2 Latest version!	Homo sapiens endoplasmic reticulum to nucleus signaling 1 (ERN1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OHu86686	XM_017024347.2 Latest version!	Homo sapiens endoplasmic reticulum to nucleus signaling 1 (ERN1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
View Old Accession Versions >>
OHu25357	NM_001433.4	Homo sapiens endoplasmic reticulum to nucleus signaling 1 (ERN1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu25357	NM_001433.3	Homo sapiens endoplasmic reticulum to nucleus signaling 1 (ERN1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu86687	XM_017024348.1	Homo sapiens endoplasmic reticulum to nucleus signaling 1 (ERN1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OHu86686	XM_017024347.1	Homo sapiens endoplasmic reticulum to nucleus signaling 1 (ERN1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ERN1 ( NM_001433.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_001424.3 Homo sapiens endoplasmic reticulum to nucleus signaling 1 (ERN1), mRNA.

Price & Availability↑

CloneID	OHu25357
Clone ID Related Accession (Same CDS sequence)	NM_001433.4 , NM_001433.5 , NM_001433.3
Accession Version	NM_001433.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2934bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-31
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase/endoribonuclease IRE1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB128514.1, AB209869.1, AC005803.1, BC130405.1, BC070350.1 and CA311378.1. On Aug 29, 2017 this sequence version replaced NM_001433.3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB209869.1, AF059198.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

ATGCCGGCCC GGCGGCTGCT GCTGCTGCTG ACGCTGCTGC TGCCCGGCCT CGGGATTTTT 
GGAAGTACCA GCACAGTGAC GCTTCCTGAA ACCTTGTTGT TTGTGTCAAC GCTGGATGGA 
AGTTTGCATG CTGTCAGCAA GAGGACAGGC TCAATCAAAT GGACTTTAAA AGAAGATCCA 
GTCCTGCAGG TCCCAACACA TGTGGAAGAG CCTGCCTTTC TCCCAGATCC TAATGATGGC 
AGCCTGTATA CGCTTGGAAG CAAGAATAAT GAAGGCCTGA CGAAACTTCC TTTTACCATC 
CCAGAATTGG TGCAGGCATC CCCATGCCGA AGTTCAGATG GAATCCTCTA CATGGGTAAA 
AAGCAGGACA TCTGGTATGT TATTGACCTC CTGACCGGAG AGAAGCAGCA GACTTTGTCA 
TCGGCCTTTG CAGATAGTCT CTGCCCATCA ACCTCTCTTC TGTATCTTGG GCGAACAGAA 
TACACCATCA CCATGTACGA CACCAAAACC CGAGAGCTCC GGTGGAATGC CACCTACTTT 
GACTATGCGG CCTCACTGCC TGAGGACGAC GTGGACTACA AGATGTCCCA CTTTGTGTCC 
AATGGTGATG GGCTGGTGGT GACTGTGGAC AGTGAATCTG GGGACGTCCT GTGGATCCAA 
AACTACGCCT CCCCTGTGGT GGCCTTTTAT GTCTGGCAGC GGGAGGGTCT GAGGAAGGTG 
ATGCACATCA ATGTCGCTGT GGAGACCCTG CGCTATCTGA CCTTCATGTC TGGGGAGGTG 
GGGCGCATCA CAAAGTGGAA GTACCCGTTC CCCAAGGAGA CAGAGGCCAA GAGCAAGCTG 
ACGCCCACTC TGTATGTTGG GAAATACTCT ACCAGCCTCT ATGCCTCTCC CTCAATGGTA 
CACGAGGGGG TTGCTGTCGT GCCCCGCGGC AGCACACTTC CTTTGCTGGA AGGGCCCCAG 
ACTGATGGCG TCACCATTGG GGACAAGGGG GAGTGTGTGA TCACGCCCAG CACGGACGTC 
AAGTTTGATC CCGGACTCAA AAGCAAGAAC AAGCTCAACT ACTTGAGGAA TTACTGGCTT 
CTGATAGGAC ACCATGAAAC CCCACTGTCT GCGTCTACCA AGATGCTGGA GAGATTTCCC 
AACAATCTAC CCAAACATCG GGAAAATGTG ATTCCTGCTG ATTCAGAGAA AAAGAGCTTT 
GAGGAAGTTA TCAACCTGGT TGACCAGACT TCAGAAAACG CACCTACCAC CGTGTCTCGG 
GATGTGGAGG AGAAGCCCGC CCATGCCCCT GCCCGGCCCG AGGCCCCCGT GGACTCCATG 
CTTAAGGACA TGGCTACCAT CATCCTGAGC ACCTTCCTGC TGATTGGCTG GGTGGCCTTC 
ATCATCACCT ATCCCCTGAG CATGCATCAG CAGCAGCAGC TCCAGCACCA GCAGTTCCAG 
AAGGAACTGG AGAAGATCCA GCTCCTGCAG CAGCAGCAGC AGCAGCTGCC CTTCCACCCA 
CCTGGAGACA CGGCTCAGGA CGGCGAGCTC CTGGACACGT CTGGCCCGTA CTCAGAGAGC 
TCGGGCACCA GCAGCCCCAG CACGTCCCCC AGGGCCTCCA ACCACTCGCT CTGCTCCGGC 
AGCTCTGCCT CCAAGGCTGG CAGCAGCCCC TCCCTGGAAC AAGACGATGG AGATGAGGAA 
ACCAGCGTGG TGATAGTTGG GAAAATTTCC TTCTGTCCCA AGGATGTCCT GGGCCATGGA 
GCTGAGGGCA CAATTGTGTA CCGGGGCATG TTTGACAACC GCGACGTGGC CGTGAAGAGG 
ATCCTCCCCG AGTGTTTTAG CTTCGCAGAC CGTGAGGTCC AGCTGTTGCG AGAATCGGAT 
GAGCACCCGA ACGTGATCCG CTACTTCTGC ACGGAGAAGG ACCGGCAATT CCAGTACATT 
GCCATCGAGC TGTGTGCAGC CACCCTGCAA GAGTATGTGG AGCAGAAGGA CTTTGCGCAT 
CTCGGCCTGG AGCCCATCAC CTTGCTGCAG CAGACCACCT CGGGCCTGGC CCACCTCCAC 
TCCCTCAACA TCGTTCACAG AGACCTAAAG CCACACAACA TCCTCATATC CATGCCCAAT 
GCACACGGCA AGATCAAGGC CATGATCTCC GACTTTGGCC TCTGCAAGAA GCTGGCAGTG 
GGCAGACACA GTTTCAGCCG CCGATCTGGG GTGCCTGGCA CAGAAGGCTG GATCGCTCCA 
GAGATGCTGA GCGAAGACTG TAAGGAGAAC CCTACCTACA CGGTGGACAT CTTTTCTGCA 
GGCTGCGTCT TTTACTACGT AATCTCTGAG GGCAGCCACC CTTTTGGCAA GTCCCTGCAG 
CGGCAGGCCA ACATCCTCCT GGGTGCCTGC AGCCTTGACT GCTTGCACCC AGAGAAGCAC 
GAAGACGTCA TTGCACGTGA ATTGATAGAG AAGATGATTG CGATGGATCC TCAGAAACGC 
CCCTCAGCGA AGCATGTGCT CAAACACCCG TTCTTCTGGA GCCTAGAGAA GCAGCTCCAG 
TTCTTCCAGG ACGTGAGCGA CAGAATAGAA AAGGAATCCC TGGATGGCCC GATCGTGAAG 
CAGTTAGAGA GAGGCGGGAG AGCCGTGGTG AAGATGGACT GGCGGGAGAA CATCACTGTC 
CCCCTCCAGA CAGACCTGCG TAAATTCAGG ACCTATAAAG GTGGTTCTGT CAGAGATCTC 
CTCCGAGCCA TGAGAAATAA GAAGCACCAC TACCGGGAGC TGCCTGCAGA GGTGCGGGAG 
ACGCTGGGGT CCCTCCCCGA CGACTTCGTG TGCTACTTCA CATCTCGCTT CCCCCACCTC 
CTCGCACACA CCTACCGGGC CATGGAGCTG TGCAGCCACG AGAGACTCTT CCAGCCCTAC 
TACTTCCACG AGCCCCCAGA GCCCCAGCCC CCAGTGACTC CAGACGCCCT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001424.3
CDS	114..3047
Translation	MPARRLLLLLTLLLPGLGIFGSTSTVTLPETLLFVSTLDGSLHAVSKRTGSIKWTLKEDPVLQVPTHVEEPAFLPDPNDGSLYTLGSKNNEGLTKLPFTIPELVQASPCRSSDGILYMGKKQDIWYVIDLLTGEKQQTLSSAFADSLCPSTSLLYLGRTEYTITMYDTKTRELRWNATYFDYAASLPEDDVDYKMSHFVSNGDGLVVTVDSESGDVLWIQNYASPVVAFYVWQREGLRKVMHINVAVETLRYLTFMSGEVGRITKWKYPFPKETEAKSKLTPTLYVGKYSTSLYASPSMVHEGVAVVPRGSTLPLLEGPQTDGVTIGDKGECVITPSTDVKFDPGLKSKNKLNYLRNYWLLIGHHETPLSASTKMLERFPNNLPKHRENVIPADSEKKSFEEVINLVDQTSENAPTTVSRDVEEKPAHAPARPEAPVDSMLKDMATIILSTFLLIGWVAFIITYPLSMHQQQQLQHQQFQKELEKIQLLQQQQQQLPFHPPGDTAQDGELLDTSGPYSESSGTSSPSTSPRASNHSLCSGSSASKAGSSPSLEQDDGDEETSVVIVGKISFCPKDVLGHGAEGTIVYRGMFDNRDVAVKRILPECFSFADREVQLLRESDEHPNVIRYFCTEKDRQFQYIAIELCAATLQEYVEQKDFAHLGLEPITLLQQTTSGLAHLHSLNIVHRDLKPHNILISMPNAHGKIKAMISDFGLCKKLAVGRHSFSRRSGVPGTEGWIAPEMLSEDCKENPTYTVDIFSAGCVFYYVISEGSHPFGKSLQRQANILLGACSLDCLHPEKHEDVIARELIEKMIAMDPQKRPSAKHVLKHPFFWSLEKQLQFFQDVSDRIEKESLDGPIVKQLERGGRAVVKMDWRENITVPLQTDLRKFRTYKGGSVRDLLRAMRNKKHHYRELPAEVRETLGSLPDDFVCYFTSRFPHLLAHTYRAMELCSHERLFQPYYFHEPPEPQPPVTPDAL

Target ORF information:

RefSeq Version	NM_001433.4
Organism	Homo sapiens(human)
Definition	Homo sapiens endoplasmic reticulum to nucleus signaling 1 (ERN1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001433.4

ORF Insert Sequence:

ATGCCGGCCC GGCGGCTGCT GCTGCTGCTG ACGCTGCTGC TGCCCGGCCT CGGGATTTTT 
GGAAGTACCA GCACAGTGAC GCTTCCTGAA ACCTTGTTGT TTGTGTCAAC GCTGGATGGA 
AGTTTGCATG CTGTCAGCAA GAGGACAGGC TCAATCAAAT GGACTTTAAA AGAAGATCCA 
GTCCTGCAGG TCCCAACACA TGTGGAAGAG CCTGCCTTTC TCCCAGATCC TAATGATGGC 
AGCCTGTATA CGCTTGGAAG CAAGAATAAT GAAGGCCTGA CGAAACTTCC TTTTACCATC 
CCAGAATTGG TGCAGGCATC CCCATGCCGA AGTTCAGATG GAATCCTCTA CATGGGTAAA 
AAGCAGGACA TCTGGTATGT TATTGACCTC CTGACCGGAG AGAAGCAGCA GACTTTGTCA 
TCGGCCTTTG CAGATAGTCT CTGCCCATCA ACCTCTCTTC TGTATCTTGG GCGAACAGAA 
TACACCATCA CCATGTACGA CACCAAAACC CGAGAGCTCC GGTGGAATGC CACCTACTTT 
GACTATGCGG CCTCACTGCC TGAGGACGAC GTGGACTACA AGATGTCCCA CTTTGTGTCC 
AATGGTGATG GGCTGGTGGT GACTGTGGAC AGTGAATCTG GGGACGTCCT GTGGATCCAA 
AACTACGCCT CCCCTGTGGT GGCCTTTTAT GTCTGGCAGC GGGAGGGTCT GAGGAAGGTG 
ATGCACATCA ATGTCGCTGT GGAGACCCTG CGCTATCTGA CCTTCATGTC TGGGGAGGTG 
GGGCGCATCA CAAAGTGGAA GTACCCGTTC CCCAAGGAGA CAGAGGCCAA GAGCAAGCTG 
ACGCCCACTC TGTATGTTGG GAAATACTCT ACCAGCCTCT ATGCCTCTCC CTCAATGGTA 
CACGAGGGGG TTGCTGTCGT GCCCCGCGGC AGCACACTTC CTTTGCTGGA AGGGCCCCAG 
ACTGATGGCG TCACCATTGG GGACAAGGGG GAGTGTGTGA TCACGCCCAG CACGGACGTC 
AAGTTTGATC CCGGACTCAA AAGCAAGAAC AAGCTCAACT ACTTGAGGAA TTACTGGCTT 
CTGATAGGAC ACCATGAAAC CCCACTGTCT GCGTCTACCA AGATGCTGGA GAGATTTCCC 
AACAATCTAC CCAAACATCG GGAAAATGTG ATTCCTGCTG ATTCAGAGAA AAAGAGCTTT 
GAGGAAGTTA TCAACCTGGT TGACCAGACT TCAGAAAACG CACCTACCAC CGTGTCTCGG 
GATGTGGAGG AGAAGCCCGC CCATGCCCCT GCCCGGCCCG AGGCCCCCGT GGACTCCATG 
CTTAAGGACA TGGCTACCAT CATCCTGAGC ACCTTCCTGC TGATTGGCTG GGTGGCCTTC 
ATCATCACCT ATCCCCTGAG CATGCATCAG CAGCAGCAGC TCCAGCACCA GCAGTTCCAG 
AAGGAACTGG AGAAGATCCA GCTCCTGCAG CAGCAGCAGC AGCAGCTGCC CTTCCACCCA 
CCTGGAGACA CGGCTCAGGA CGGCGAGCTC CTGGACACGT CTGGCCCGTA CTCAGAGAGC 
TCGGGCACCA GCAGCCCCAG CACGTCCCCC AGGGCCTCCA ACCACTCGCT CTGCTCCGGC 
AGCTCTGCCT CCAAGGCTGG CAGCAGCCCC TCCCTGGAAC AAGACGATGG AGATGAGGAA 
ACCAGCGTGG TGATAGTTGG GAAAATTTCC TTCTGTCCCA AGGATGTCCT GGGCCATGGA 
GCTGAGGGCA CAATTGTGTA CCGGGGCATG TTTGACAACC GCGACGTGGC CGTGAAGAGG 
ATCCTCCCCG AGTGTTTTAG CTTCGCAGAC CGTGAGGTCC AGCTGTTGCG AGAATCGGAT 
GAGCACCCGA ACGTGATCCG CTACTTCTGC ACGGAGAAGG ACCGGCAATT CCAGTACATT 
GCCATCGAGC TGTGTGCAGC CACCCTGCAA GAGTATGTGG AGCAGAAGGA CTTTGCGCAT 
CTCGGCCTGG AGCCCATCAC CTTGCTGCAG CAGACCACCT CGGGCCTGGC CCACCTCCAC 
TCCCTCAACA TCGTTCACAG AGACCTAAAG CCACACAACA TCCTCATATC CATGCCCAAT 
GCACACGGCA AGATCAAGGC CATGATCTCC GACTTTGGCC TCTGCAAGAA GCTGGCAGTG 
GGCAGACACA GTTTCAGCCG CCGATCTGGG GTGCCTGGCA CAGAAGGCTG GATCGCTCCA 
GAGATGCTGA GCGAAGACTG TAAGGAGAAC CCTACCTACA CGGTGGACAT CTTTTCTGCA 
GGCTGCGTCT TTTACTACGT AATCTCTGAG GGCAGCCACC CTTTTGGCAA GTCCCTGCAG 
CGGCAGGCCA ACATCCTCCT GGGTGCCTGC AGCCTTGACT GCTTGCACCC AGAGAAGCAC 
GAAGACGTCA TTGCACGTGA ATTGATAGAG AAGATGATTG CGATGGATCC TCAGAAACGC 
CCCTCAGCGA AGCATGTGCT CAAACACCCG TTCTTCTGGA GCCTAGAGAA GCAGCTCCAG 
TTCTTCCAGG ACGTGAGCGA CAGAATAGAA AAGGAATCCC TGGATGGCCC GATCGTGAAG 
CAGTTAGAGA GAGGCGGGAG AGCCGTGGTG AAGATGGACT GGCGGGAGAA CATCACTGTC 
CCCCTCCAGA CAGACCTGCG TAAATTCAGG ACCTATAAAG GTGGTTCTGT CAGAGATCTC 
CTCCGAGCCA TGAGAAATAA GAAGCACCAC TACCGGGAGC TGCCTGCAGA GGTGCGGGAG 
ACGCTGGGGT CCCTCCCCGA CGACTTCGTG TGCTACTTCA CATCTCGCTT CCCCCACCTC 
CTCGCACACA CCTACCGGGC CATGGAGCTG TGCAGCCACG AGAGACTCTT CCAGCCCTAC 
TACTTCCACG AGCCCCCAGA GCCCCAGCCC CCAGTGACTC CAGACGCCCT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ERN1 ( NM_001433.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_001424.3 Homo sapiens endoplasmic reticulum to nucleus signaling 1 (ERN1), mRNA.

Price & Availability↑

CloneID	OHu25357
Clone ID Related Accession (Same CDS sequence)	NM_001433.4 , NM_001433.5 , NM_001433.3
Accession Version	NM_001433.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2934bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-01
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase/endoribonuclease IRE1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC025362.12, DB128514.1, AB209869.1, AC005803.1, BC130405.1, BC070350.1 and CA311378.1. On Nov 23, 2018 this sequence version replaced NM_001433.4. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.231264.1, AB209869.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000433197.4/ ENSP00000401445.2 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCCGGCCC GGCGGCTGCT GCTGCTGCTG ACGCTGCTGC TGCCCGGCCT CGGGATTTTT 
GGAAGTACCA GCACAGTGAC GCTTCCTGAA ACCTTGTTGT TTGTGTCAAC GCTGGATGGA 
AGTTTGCATG CTGTCAGCAA GAGGACAGGC TCAATCAAAT GGACTTTAAA AGAAGATCCA 
GTCCTGCAGG TCCCAACACA TGTGGAAGAG CCTGCCTTTC TCCCAGATCC TAATGATGGC 
AGCCTGTATA CGCTTGGAAG CAAGAATAAT GAAGGCCTGA CGAAACTTCC TTTTACCATC 
CCAGAATTGG TGCAGGCATC CCCATGCCGA AGTTCAGATG GAATCCTCTA CATGGGTAAA 
AAGCAGGACA TCTGGTATGT TATTGACCTC CTGACCGGAG AGAAGCAGCA GACTTTGTCA 
TCGGCCTTTG CAGATAGTCT CTGCCCATCA ACCTCTCTTC TGTATCTTGG GCGAACAGAA 
TACACCATCA CCATGTACGA CACCAAAACC CGAGAGCTCC GGTGGAATGC CACCTACTTT 
GACTATGCGG CCTCACTGCC TGAGGACGAC GTGGACTACA AGATGTCCCA CTTTGTGTCC 
AATGGTGATG GGCTGGTGGT GACTGTGGAC AGTGAATCTG GGGACGTCCT GTGGATCCAA 
AACTACGCCT CCCCTGTGGT GGCCTTTTAT GTCTGGCAGC GGGAGGGTCT GAGGAAGGTG 
ATGCACATCA ATGTCGCTGT GGAGACCCTG CGCTATCTGA CCTTCATGTC TGGGGAGGTG 
GGGCGCATCA CAAAGTGGAA GTACCCGTTC CCCAAGGAGA CAGAGGCCAA GAGCAAGCTG 
ACGCCCACTC TGTATGTTGG GAAATACTCT ACCAGCCTCT ATGCCTCTCC CTCAATGGTA 
CACGAGGGGG TTGCTGTCGT GCCCCGCGGC AGCACACTTC CTTTGCTGGA AGGGCCCCAG 
ACTGATGGCG TCACCATTGG GGACAAGGGG GAGTGTGTGA TCACGCCCAG CACGGACGTC 
AAGTTTGATC CCGGACTCAA AAGCAAGAAC AAGCTCAACT ACTTGAGGAA TTACTGGCTT 
CTGATAGGAC ACCATGAAAC CCCACTGTCT GCGTCTACCA AGATGCTGGA GAGATTTCCC 
AACAATCTAC CCAAACATCG GGAAAATGTG ATTCCTGCTG ATTCAGAGAA AAAGAGCTTT 
GAGGAAGTTA TCAACCTGGT TGACCAGACT TCAGAAAACG CACCTACCAC CGTGTCTCGG 
GATGTGGAGG AGAAGCCCGC CCATGCCCCT GCCCGGCCCG AGGCCCCCGT GGACTCCATG 
CTTAAGGACA TGGCTACCAT CATCCTGAGC ACCTTCCTGC TGATTGGCTG GGTGGCCTTC 
ATCATCACCT ATCCCCTGAG CATGCATCAG CAGCAGCAGC TCCAGCACCA GCAGTTCCAG 
AAGGAACTGG AGAAGATCCA GCTCCTGCAG CAGCAGCAGC AGCAGCTGCC CTTCCACCCA 
CCTGGAGACA CGGCTCAGGA CGGCGAGCTC CTGGACACGT CTGGCCCGTA CTCAGAGAGC 
TCGGGCACCA GCAGCCCCAG CACGTCCCCC AGGGCCTCCA ACCACTCGCT CTGCTCCGGC 
AGCTCTGCCT CCAAGGCTGG CAGCAGCCCC TCCCTGGAAC AAGACGATGG AGATGAGGAA 
ACCAGCGTGG TGATAGTTGG GAAAATTTCC TTCTGTCCCA AGGATGTCCT GGGCCATGGA 
GCTGAGGGCA CAATTGTGTA CCGGGGCATG TTTGACAACC GCGACGTGGC CGTGAAGAGG 
ATCCTCCCCG AGTGTTTTAG CTTCGCAGAC CGTGAGGTCC AGCTGTTGCG AGAATCGGAT 
GAGCACCCGA ACGTGATCCG CTACTTCTGC ACGGAGAAGG ACCGGCAATT CCAGTACATT 
GCCATCGAGC TGTGTGCAGC CACCCTGCAA GAGTATGTGG AGCAGAAGGA CTTTGCGCAT 
CTCGGCCTGG AGCCCATCAC CTTGCTGCAG CAGACCACCT CGGGCCTGGC CCACCTCCAC 
TCCCTCAACA TCGTTCACAG AGACCTAAAG CCACACAACA TCCTCATATC CATGCCCAAT 
GCACACGGCA AGATCAAGGC CATGATCTCC GACTTTGGCC TCTGCAAGAA GCTGGCAGTG 
GGCAGACACA GTTTCAGCCG CCGATCTGGG GTGCCTGGCA CAGAAGGCTG GATCGCTCCA 
GAGATGCTGA GCGAAGACTG TAAGGAGAAC CCTACCTACA CGGTGGACAT CTTTTCTGCA 
GGCTGCGTCT TTTACTACGT AATCTCTGAG GGCAGCCACC CTTTTGGCAA GTCCCTGCAG 
CGGCAGGCCA ACATCCTCCT GGGTGCCTGC AGCCTTGACT GCTTGCACCC AGAGAAGCAC 
GAAGACGTCA TTGCACGTGA ATTGATAGAG AAGATGATTG CGATGGATCC TCAGAAACGC 
CCCTCAGCGA AGCATGTGCT CAAACACCCG TTCTTCTGGA GCCTAGAGAA GCAGCTCCAG 
TTCTTCCAGG ACGTGAGCGA CAGAATAGAA AAGGAATCCC TGGATGGCCC GATCGTGAAG 
CAGTTAGAGA GAGGCGGGAG AGCCGTGGTG AAGATGGACT GGCGGGAGAA CATCACTGTC 
CCCCTCCAGA CAGACCTGCG TAAATTCAGG ACCTATAAAG GTGGTTCTGT CAGAGATCTC 
CTCCGAGCCA TGAGAAATAA GAAGCACCAC TACCGGGAGC TGCCTGCAGA GGTGCGGGAG 
ACGCTGGGGT CCCTCCCCGA CGACTTCGTG TGCTACTTCA CATCTCGCTT CCCCCACCTC 
CTCGCACACA CCTACCGGGC CATGGAGCTG TGCAGCCACG AGAGACTCTT CCAGCCCTAC 
TACTTCCACG AGCCCCCAGA GCCCCAGCCC CCAGTGACTC CAGACGCCCT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001424.3
CDS	116..3049
Translation	MPARRLLLLLTLLLPGLGIFGSTSTVTLPETLLFVSTLDGSLHAVSKRTGSIKWTLKEDPVLQVPTHVEEPAFLPDPNDGSLYTLGSKNNEGLTKLPFTIPELVQASPCRSSDGILYMGKKQDIWYVIDLLTGEKQQTLSSAFADSLCPSTSLLYLGRTEYTITMYDTKTRELRWNATYFDYAASLPEDDVDYKMSHFVSNGDGLVVTVDSESGDVLWIQNYASPVVAFYVWQREGLRKVMHINVAVETLRYLTFMSGEVGRITKWKYPFPKETEAKSKLTPTLYVGKYSTSLYASPSMVHEGVAVVPRGSTLPLLEGPQTDGVTIGDKGECVITPSTDVKFDPGLKSKNKLNYLRNYWLLIGHHETPLSASTKMLERFPNNLPKHRENVIPADSEKKSFEEVINLVDQTSENAPTTVSRDVEEKPAHAPARPEAPVDSMLKDMATIILSTFLLIGWVAFIITYPLSMHQQQQLQHQQFQKELEKIQLLQQQQQQLPFHPPGDTAQDGELLDTSGPYSESSGTSSPSTSPRASNHSLCSGSSASKAGSSPSLEQDDGDEETSVVIVGKISFCPKDVLGHGAEGTIVYRGMFDNRDVAVKRILPECFSFADREVQLLRESDEHPNVIRYFCTEKDRQFQYIAIELCAATLQEYVEQKDFAHLGLEPITLLQQTTSGLAHLHSLNIVHRDLKPHNILISMPNAHGKIKAMISDFGLCKKLAVGRHSFSRRSGVPGTEGWIAPEMLSEDCKENPTYTVDIFSAGCVFYYVISEGSHPFGKSLQRQANILLGACSLDCLHPEKHEDVIARELIEKMIAMDPQKRPSAKHVLKHPFFWSLEKQLQFFQDVSDRIEKESLDGPIVKQLERGGRAVVKMDWRENITVPLQTDLRKFRTYKGGSVRDLLRAMRNKKHHYRELPAEVRETLGSLPDDFVCYFTSRFPHLLAHTYRAMELCSHERLFQPYYFHEPPEPQPPVTPDAL

Target ORF information:

RefSeq Version	NM_001433.5
Organism	Homo sapiens(human)
Definition	Homo sapiens endoplasmic reticulum to nucleus signaling 1 (ERN1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001433.5

ORF Insert Sequence:

ATGCCGGCCC GGCGGCTGCT GCTGCTGCTG ACGCTGCTGC TGCCCGGCCT CGGGATTTTT 
GGAAGTACCA GCACAGTGAC GCTTCCTGAA ACCTTGTTGT TTGTGTCAAC GCTGGATGGA 
AGTTTGCATG CTGTCAGCAA GAGGACAGGC TCAATCAAAT GGACTTTAAA AGAAGATCCA 
GTCCTGCAGG TCCCAACACA TGTGGAAGAG CCTGCCTTTC TCCCAGATCC TAATGATGGC 
AGCCTGTATA CGCTTGGAAG CAAGAATAAT GAAGGCCTGA CGAAACTTCC TTTTACCATC 
CCAGAATTGG TGCAGGCATC CCCATGCCGA AGTTCAGATG GAATCCTCTA CATGGGTAAA 
AAGCAGGACA TCTGGTATGT TATTGACCTC CTGACCGGAG AGAAGCAGCA GACTTTGTCA 
TCGGCCTTTG CAGATAGTCT CTGCCCATCA ACCTCTCTTC TGTATCTTGG GCGAACAGAA 
TACACCATCA CCATGTACGA CACCAAAACC CGAGAGCTCC GGTGGAATGC CACCTACTTT 
GACTATGCGG CCTCACTGCC TGAGGACGAC GTGGACTACA AGATGTCCCA CTTTGTGTCC 
AATGGTGATG GGCTGGTGGT GACTGTGGAC AGTGAATCTG GGGACGTCCT GTGGATCCAA 
AACTACGCCT CCCCTGTGGT GGCCTTTTAT GTCTGGCAGC GGGAGGGTCT GAGGAAGGTG 
ATGCACATCA ATGTCGCTGT GGAGACCCTG CGCTATCTGA CCTTCATGTC TGGGGAGGTG 
GGGCGCATCA CAAAGTGGAA GTACCCGTTC CCCAAGGAGA CAGAGGCCAA GAGCAAGCTG 
ACGCCCACTC TGTATGTTGG GAAATACTCT ACCAGCCTCT ATGCCTCTCC CTCAATGGTA 
CACGAGGGGG TTGCTGTCGT GCCCCGCGGC AGCACACTTC CTTTGCTGGA AGGGCCCCAG 
ACTGATGGCG TCACCATTGG GGACAAGGGG GAGTGTGTGA TCACGCCCAG CACGGACGTC 
AAGTTTGATC CCGGACTCAA AAGCAAGAAC AAGCTCAACT ACTTGAGGAA TTACTGGCTT 
CTGATAGGAC ACCATGAAAC CCCACTGTCT GCGTCTACCA AGATGCTGGA GAGATTTCCC 
AACAATCTAC CCAAACATCG GGAAAATGTG ATTCCTGCTG ATTCAGAGAA AAAGAGCTTT 
GAGGAAGTTA TCAACCTGGT TGACCAGACT TCAGAAAACG CACCTACCAC CGTGTCTCGG 
GATGTGGAGG AGAAGCCCGC CCATGCCCCT GCCCGGCCCG AGGCCCCCGT GGACTCCATG 
CTTAAGGACA TGGCTACCAT CATCCTGAGC ACCTTCCTGC TGATTGGCTG GGTGGCCTTC 
ATCATCACCT ATCCCCTGAG CATGCATCAG CAGCAGCAGC TCCAGCACCA GCAGTTCCAG 
AAGGAACTGG AGAAGATCCA GCTCCTGCAG CAGCAGCAGC AGCAGCTGCC CTTCCACCCA 
CCTGGAGACA CGGCTCAGGA CGGCGAGCTC CTGGACACGT CTGGCCCGTA CTCAGAGAGC 
TCGGGCACCA GCAGCCCCAG CACGTCCCCC AGGGCCTCCA ACCACTCGCT CTGCTCCGGC 
AGCTCTGCCT CCAAGGCTGG CAGCAGCCCC TCCCTGGAAC AAGACGATGG AGATGAGGAA 
ACCAGCGTGG TGATAGTTGG GAAAATTTCC TTCTGTCCCA AGGATGTCCT GGGCCATGGA 
GCTGAGGGCA CAATTGTGTA CCGGGGCATG TTTGACAACC GCGACGTGGC CGTGAAGAGG 
ATCCTCCCCG AGTGTTTTAG CTTCGCAGAC CGTGAGGTCC AGCTGTTGCG AGAATCGGAT 
GAGCACCCGA ACGTGATCCG CTACTTCTGC ACGGAGAAGG ACCGGCAATT CCAGTACATT 
GCCATCGAGC TGTGTGCAGC CACCCTGCAA GAGTATGTGG AGCAGAAGGA CTTTGCGCAT 
CTCGGCCTGG AGCCCATCAC CTTGCTGCAG CAGACCACCT CGGGCCTGGC CCACCTCCAC 
TCCCTCAACA TCGTTCACAG AGACCTAAAG CCACACAACA TCCTCATATC CATGCCCAAT 
GCACACGGCA AGATCAAGGC CATGATCTCC GACTTTGGCC TCTGCAAGAA GCTGGCAGTG 
GGCAGACACA GTTTCAGCCG CCGATCTGGG GTGCCTGGCA CAGAAGGCTG GATCGCTCCA 
GAGATGCTGA GCGAAGACTG TAAGGAGAAC CCTACCTACA CGGTGGACAT CTTTTCTGCA 
GGCTGCGTCT TTTACTACGT AATCTCTGAG GGCAGCCACC CTTTTGGCAA GTCCCTGCAG 
CGGCAGGCCA ACATCCTCCT GGGTGCCTGC AGCCTTGACT GCTTGCACCC AGAGAAGCAC 
GAAGACGTCA TTGCACGTGA ATTGATAGAG AAGATGATTG CGATGGATCC TCAGAAACGC 
CCCTCAGCGA AGCATGTGCT CAAACACCCG TTCTTCTGGA GCCTAGAGAA GCAGCTCCAG 
TTCTTCCAGG ACGTGAGCGA CAGAATAGAA AAGGAATCCC TGGATGGCCC GATCGTGAAG 
CAGTTAGAGA GAGGCGGGAG AGCCGTGGTG AAGATGGACT GGCGGGAGAA CATCACTGTC 
CCCCTCCAGA CAGACCTGCG TAAATTCAGG ACCTATAAAG GTGGTTCTGT CAGAGATCTC 
CTCCGAGCCA TGAGAAATAA GAAGCACCAC TACCGGGAGC TGCCTGCAGA GGTGCGGGAG 
ACGCTGGGGT CCCTCCCCGA CGACTTCGTG TGCTACTTCA CATCTCGCTT CCCCCACCTC 
CTCGCACACA CCTACCGGGC CATGGAGCTG TGCAGCCACG AGAGACTCTT CCAGCCCTAC 
TACTTCCACG AGCCCCCAGA GCCCCAGCCC CCAGTGACTC CAGACGCCCT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ERN1 ( NM_001433.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001424.3 Homo sapiens endoplasmic reticulum to nucleus signaling 1 (ERN1), mRNA.

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CloneID	OHu25357
Clone ID Related Accession (Same CDS sequence)	NM_001433.4 , NM_001433.5 , NM_001433.3
Accession Version	NM_001433.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2934bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-05
Organism	Homo sapiens(Human)
Product	serine/threonine-protein kinase/endoribonuclease IRE1 precursor
Comment	REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB128514.1, AB209869.1, AC005803.1 and BC130405.1. On Jul 26, 2007 this sequence version replaced NM_001433.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.231264.1, AB209869.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

ATGCCGGCCC GGCGGCTGCT GCTGCTGCTG ACGCTGCTGC TGCCCGGCCT CGGGATTTTT 
GGAAGTACCA GCACAGTGAC GCTTCCTGAA ACCTTGTTGT TTGTGTCAAC GCTGGATGGA 
AGTTTGCATG CTGTCAGCAA GAGGACAGGC TCAATCAAAT GGACTTTAAA AGAAGATCCA 
GTCCTGCAGG TCCCAACACA TGTGGAAGAG CCTGCCTTTC TCCCAGATCC TAATGATGGC 
AGCCTGTATA CGCTTGGAAG CAAGAATAAT GAAGGCCTGA CGAAACTTCC TTTTACCATC 
CCAGAATTGG TGCAGGCATC CCCATGCCGA AGTTCAGATG GAATCCTCTA CATGGGTAAA 
AAGCAGGACA TCTGGTATGT TATTGACCTC CTGACCGGAG AGAAGCAGCA GACTTTGTCA 
TCGGCCTTTG CAGATAGTCT CTGCCCATCA ACCTCTCTTC TGTATCTTGG GCGAACAGAA 
TACACCATCA CCATGTACGA CACCAAAACC CGAGAGCTCC GGTGGAATGC CACCTACTTT 
GACTATGCGG CCTCACTGCC TGAGGACGAC GTGGACTACA AGATGTCCCA CTTTGTGTCC 
AATGGTGATG GGCTGGTGGT GACTGTGGAC AGTGAATCTG GGGACGTCCT GTGGATCCAA 
AACTACGCCT CCCCTGTGGT GGCCTTTTAT GTCTGGCAGC GGGAGGGTCT GAGGAAGGTG 
ATGCACATCA ATGTCGCTGT GGAGACCCTG CGCTATCTGA CCTTCATGTC TGGGGAGGTG 
GGGCGCATCA CAAAGTGGAA GTACCCGTTC CCCAAGGAGA CAGAGGCCAA GAGCAAGCTG 
ACGCCCACTC TGTATGTTGG GAAATACTCT ACCAGCCTCT ATGCCTCTCC CTCAATGGTA 
CACGAGGGGG TTGCTGTCGT GCCCCGCGGC AGCACACTTC CTTTGCTGGA AGGGCCCCAG 
ACTGATGGCG TCACCATTGG GGACAAGGGG GAGTGTGTGA TCACGCCCAG CACGGACGTC 
AAGTTTGATC CCGGACTCAA AAGCAAGAAC AAGCTCAACT ACTTGAGGAA TTACTGGCTT 
CTGATAGGAC ACCATGAAAC CCCACTGTCT GCGTCTACCA AGATGCTGGA GAGATTTCCC 
AACAATCTAC CCAAACATCG GGAAAATGTG ATTCCTGCTG ATTCAGAGAA AAAGAGCTTT 
GAGGAAGTTA TCAACCTGGT TGACCAGACT TCAGAAAACG CACCTACCAC CGTGTCTCGG 
GATGTGGAGG AGAAGCCCGC CCATGCCCCT GCCCGGCCCG AGGCCCCCGT GGACTCCATG 
CTTAAGGACA TGGCTACCAT CATCCTGAGC ACCTTCCTGC TGATTGGCTG GGTGGCCTTC 
ATCATCACCT ATCCCCTGAG CATGCATCAG CAGCAGCAGC TCCAGCACCA GCAGTTCCAG 
AAGGAACTGG AGAAGATCCA GCTCCTGCAG CAGCAGCAGC AGCAGCTGCC CTTCCACCCA 
CCTGGAGACA CGGCTCAGGA CGGCGAGCTC CTGGACACGT CTGGCCCGTA CTCAGAGAGC 
TCGGGCACCA GCAGCCCCAG CACGTCCCCC AGGGCCTCCA ACCACTCGCT CTGCTCCGGC 
AGCTCTGCCT CCAAGGCTGG CAGCAGCCCC TCCCTGGAAC AAGACGATGG AGATGAGGAA 
ACCAGCGTGG TGATAGTTGG GAAAATTTCC TTCTGTCCCA AGGATGTCCT GGGCCATGGA 
GCTGAGGGCA CAATTGTGTA CCGGGGCATG TTTGACAACC GCGACGTGGC CGTGAAGAGG 
ATCCTCCCCG AGTGTTTTAG CTTCGCAGAC CGTGAGGTCC AGCTGTTGCG AGAATCGGAT 
GAGCACCCGA ACGTGATCCG CTACTTCTGC ACGGAGAAGG ACCGGCAATT CCAGTACATT 
GCCATCGAGC TGTGTGCAGC CACCCTGCAA GAGTATGTGG AGCAGAAGGA CTTTGCGCAT 
CTCGGCCTGG AGCCCATCAC CTTGCTGCAG CAGACCACCT CGGGCCTGGC CCACCTCCAC 
TCCCTCAACA TCGTTCACAG AGACCTAAAG CCACACAACA TCCTCATATC CATGCCCAAT 
GCACACGGCA AGATCAAGGC CATGATCTCC GACTTTGGCC TCTGCAAGAA GCTGGCAGTG 
GGCAGACACA GTTTCAGCCG CCGATCTGGG GTGCCTGGCA CAGAAGGCTG GATCGCTCCA 
GAGATGCTGA GCGAAGACTG TAAGGAGAAC CCTACCTACA CGGTGGACAT CTTTTCTGCA 
GGCTGCGTCT TTTACTACGT AATCTCTGAG GGCAGCCACC CTTTTGGCAA GTCCCTGCAG 
CGGCAGGCCA ACATCCTCCT GGGTGCCTGC AGCCTTGACT GCTTGCACCC AGAGAAGCAC 
GAAGACGTCA TTGCACGTGA ATTGATAGAG AAGATGATTG CGATGGATCC TCAGAAACGC 
CCCTCAGCGA AGCATGTGCT CAAACACCCG TTCTTCTGGA GCCTAGAGAA GCAGCTCCAG 
TTCTTCCAGG ACGTGAGCGA CAGAATAGAA AAGGAATCCC TGGATGGCCC GATCGTGAAG 
CAGTTAGAGA GAGGCGGGAG AGCCGTGGTG AAGATGGACT GGCGGGAGAA CATCACTGTC 
CCCCTCCAGA CAGACCTGCG TAAATTCAGG ACCTATAAAG GTGGTTCTGT CAGAGATCTC 
CTCCGAGCCA TGAGAAATAA GAAGCACCAC TACCGGGAGC TGCCTGCAGA GGTGCGGGAG 
ACGCTGGGGT CCCTCCCCGA CGACTTCGTG TGCTACTTCA CATCTCGCTT CCCCCACCTC 
CTCGCACACA CCTACCGGGC CATGGAGCTG TGCAGCCACG AGAGACTCTT CCAGCCCTAC 
TACTTCCACG AGCCCCCAGA GCCCCAGCCC CCAGTGACTC CAGACGCCCT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001424.3
CDS	114..3047
Translation	MPARRLLLLLTLLLPGLGIFGSTSTVTLPETLLFVSTLDGSLHAVSKRTGSIKWTLKEDPVLQVPTHVEEPAFLPDPNDGSLYTLGSKNNEGLTKLPFTIPELVQASPCRSSDGILYMGKKQDIWYVIDLLTGEKQQTLSSAFADSLCPSTSLLYLGRTEYTITMYDTKTRELRWNATYFDYAASLPEDDVDYKMSHFVSNGDGLVVTVDSESGDVLWIQNYASPVVAFYVWQREGLRKVMHINVAVETLRYLTFMSGEVGRITKWKYPFPKETEAKSKLTPTLYVGKYSTSLYASPSMVHEGVAVVPRGSTLPLLEGPQTDGVTIGDKGECVITPSTDVKFDPGLKSKNKLNYLRNYWLLIGHHETPLSASTKMLERFPNNLPKHRENVIPADSEKKSFEEVINLVDQTSENAPTTVSRDVEEKPAHAPARPEAPVDSMLKDMATIILSTFLLIGWVAFIITYPLSMHQQQQLQHQQFQKELEKIQLLQQQQQQLPFHPPGDTAQDGELLDTSGPYSESSGTSSPSTSPRASNHSLCSGSSASKAGSSPSLEQDDGDEETSVVIVGKISFCPKDVLGHGAEGTIVYRGMFDNRDVAVKRILPECFSFADREVQLLRESDEHPNVIRYFCTEKDRQFQYIAIELCAATLQEYVEQKDFAHLGLEPITLLQQTTSGLAHLHSLNIVHRDLKPHNILISMPNAHGKIKAMISDFGLCKKLAVGRHSFSRRSGVPGTEGWIAPEMLSEDCKENPTYTVDIFSAGCVFYYVISEGSHPFGKSLQRQANILLGACSLDCLHPEKHEDVIARELIEKMIAMDPQKRPSAKHVLKHPFFWSLEKQLQFFQDVSDRIEKESLDGPIVKQLERGGRAVVKMDWRENITVPLQTDLRKFRTYKGGSVRDLLRAMRNKKHHYRELPAEVRETLGSLPDDFVCYFTSRFPHLLAHTYRAMELCSHERLFQPYYFHEPPEPQPPVTPDAL

Target ORF information:

RefSeq Version	NM_001433.3
Organism	Homo sapiens(Human)
Definition	Homo sapiens endoplasmic reticulum to nucleus signaling 1 (ERN1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001433.3

ORF Insert Sequence:

ATGCCGGCCC GGCGGCTGCT GCTGCTGCTG ACGCTGCTGC TGCCCGGCCT CGGGATTTTT 
GGAAGTACCA GCACAGTGAC GCTTCCTGAA ACCTTGTTGT TTGTGTCAAC GCTGGATGGA 
AGTTTGCATG CTGTCAGCAA GAGGACAGGC TCAATCAAAT GGACTTTAAA AGAAGATCCA 
GTCCTGCAGG TCCCAACACA TGTGGAAGAG CCTGCCTTTC TCCCAGATCC TAATGATGGC 
AGCCTGTATA CGCTTGGAAG CAAGAATAAT GAAGGCCTGA CGAAACTTCC TTTTACCATC 
CCAGAATTGG TGCAGGCATC CCCATGCCGA AGTTCAGATG GAATCCTCTA CATGGGTAAA 
AAGCAGGACA TCTGGTATGT TATTGACCTC CTGACCGGAG AGAAGCAGCA GACTTTGTCA 
TCGGCCTTTG CAGATAGTCT CTGCCCATCA ACCTCTCTTC TGTATCTTGG GCGAACAGAA 
TACACCATCA CCATGTACGA CACCAAAACC CGAGAGCTCC GGTGGAATGC CACCTACTTT 
GACTATGCGG CCTCACTGCC TGAGGACGAC GTGGACTACA AGATGTCCCA CTTTGTGTCC 
AATGGTGATG GGCTGGTGGT GACTGTGGAC AGTGAATCTG GGGACGTCCT GTGGATCCAA 
AACTACGCCT CCCCTGTGGT GGCCTTTTAT GTCTGGCAGC GGGAGGGTCT GAGGAAGGTG 
ATGCACATCA ATGTCGCTGT GGAGACCCTG CGCTATCTGA CCTTCATGTC TGGGGAGGTG 
GGGCGCATCA CAAAGTGGAA GTACCCGTTC CCCAAGGAGA CAGAGGCCAA GAGCAAGCTG 
ACGCCCACTC TGTATGTTGG GAAATACTCT ACCAGCCTCT ATGCCTCTCC CTCAATGGTA 
CACGAGGGGG TTGCTGTCGT GCCCCGCGGC AGCACACTTC CTTTGCTGGA AGGGCCCCAG 
ACTGATGGCG TCACCATTGG GGACAAGGGG GAGTGTGTGA TCACGCCCAG CACGGACGTC 
AAGTTTGATC CCGGACTCAA AAGCAAGAAC AAGCTCAACT ACTTGAGGAA TTACTGGCTT 
CTGATAGGAC ACCATGAAAC CCCACTGTCT GCGTCTACCA AGATGCTGGA GAGATTTCCC 
AACAATCTAC CCAAACATCG GGAAAATGTG ATTCCTGCTG ATTCAGAGAA AAAGAGCTTT 
GAGGAAGTTA TCAACCTGGT TGACCAGACT TCAGAAAACG CACCTACCAC CGTGTCTCGG 
GATGTGGAGG AGAAGCCCGC CCATGCCCCT GCCCGGCCCG AGGCCCCCGT GGACTCCATG 
CTTAAGGACA TGGCTACCAT CATCCTGAGC ACCTTCCTGC TGATTGGCTG GGTGGCCTTC 
ATCATCACCT ATCCCCTGAG CATGCATCAG CAGCAGCAGC TCCAGCACCA GCAGTTCCAG 
AAGGAACTGG AGAAGATCCA GCTCCTGCAG CAGCAGCAGC AGCAGCTGCC CTTCCACCCA 
CCTGGAGACA CGGCTCAGGA CGGCGAGCTC CTGGACACGT CTGGCCCGTA CTCAGAGAGC 
TCGGGCACCA GCAGCCCCAG CACGTCCCCC AGGGCCTCCA ACCACTCGCT CTGCTCCGGC 
AGCTCTGCCT CCAAGGCTGG CAGCAGCCCC TCCCTGGAAC AAGACGATGG AGATGAGGAA 
ACCAGCGTGG TGATAGTTGG GAAAATTTCC TTCTGTCCCA AGGATGTCCT GGGCCATGGA 
GCTGAGGGCA CAATTGTGTA CCGGGGCATG TTTGACAACC GCGACGTGGC CGTGAAGAGG 
ATCCTCCCCG AGTGTTTTAG CTTCGCAGAC CGTGAGGTCC AGCTGTTGCG AGAATCGGAT 
GAGCACCCGA ACGTGATCCG CTACTTCTGC ACGGAGAAGG ACCGGCAATT CCAGTACATT 
GCCATCGAGC TGTGTGCAGC CACCCTGCAA GAGTATGTGG AGCAGAAGGA CTTTGCGCAT 
CTCGGCCTGG AGCCCATCAC CTTGCTGCAG CAGACCACCT CGGGCCTGGC CCACCTCCAC 
TCCCTCAACA TCGTTCACAG AGACCTAAAG CCACACAACA TCCTCATATC CATGCCCAAT 
GCACACGGCA AGATCAAGGC CATGATCTCC GACTTTGGCC TCTGCAAGAA GCTGGCAGTG 
GGCAGACACA GTTTCAGCCG CCGATCTGGG GTGCCTGGCA CAGAAGGCTG GATCGCTCCA 
GAGATGCTGA GCGAAGACTG TAAGGAGAAC CCTACCTACA CGGTGGACAT CTTTTCTGCA 
GGCTGCGTCT TTTACTACGT AATCTCTGAG GGCAGCCACC CTTTTGGCAA GTCCCTGCAG 
CGGCAGGCCA ACATCCTCCT GGGTGCCTGC AGCCTTGACT GCTTGCACCC AGAGAAGCAC 
GAAGACGTCA TTGCACGTGA ATTGATAGAG AAGATGATTG CGATGGATCC TCAGAAACGC 
CCCTCAGCGA AGCATGTGCT CAAACACCCG TTCTTCTGGA GCCTAGAGAA GCAGCTCCAG 
TTCTTCCAGG ACGTGAGCGA CAGAATAGAA AAGGAATCCC TGGATGGCCC GATCGTGAAG 
CAGTTAGAGA GAGGCGGGAG AGCCGTGGTG AAGATGGACT GGCGGGAGAA CATCACTGTC 
CCCCTCCAGA CAGACCTGCG TAAATTCAGG ACCTATAAAG GTGGTTCTGT CAGAGATCTC 
CTCCGAGCCA TGAGAAATAA GAAGCACCAC TACCGGGAGC TGCCTGCAGA GGTGCGGGAG 
ACGCTGGGGT CCCTCCCCGA CGACTTCGTG TGCTACTTCA CATCTCGCTT CCCCCACCTC 
CTCGCACACA CCTACCGGGC CATGGAGCTG TGCAGCCACG AGAGACTCTT CCAGCCCTAC 
TACTTCCACG AGCCCCCAGA GCCCCAGCCC CCAGTGACTC CAGACGCCCT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ERN1 ( XM_017024348.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016879837.1 Homo sapiens endoplasmic reticulum to nucleus signaling 1 (ERN1), transcript variant X2, mRNA.

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CloneID	OHu86687
Clone ID Related Accession (Same CDS sequence)	XM_017024348.1 , XM_017024348.2
Accession Version	XM_017024348.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2943bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase/endoribonuclease IRE1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAACAGG GAGTAAGGAC TCTCACCCGA GAGCTGGCTT CTGCCACTTG CCCTGCCGGT 
TTCTTGGTAC TGGGAGGCTG CAGGCTTCCT TCTGTGCACC GAGACTTCCT TGAGCAAGTG 
GAGCTGTGGG AATTGCACTC TTGGAAGATC CCGCATGGGT TCGAGATTTT TGGAAGTACC 
AGCACAGTGA CGCTTCCTGA AACCTTGTTG TTTGTGTCAA CGCTGGATGG AAGTTTGCAT 
GCTGTCAGCA AGAGGACAGG CTCAATCAAA TGGACTTTAA AAGAAGATCC AGTCCTGCAG 
GTCCCAACAC ATGTGGAAGA GCCTGCCTTT CTCCCAGATC CTAATGATGG CAGCCTGTAT 
ACGCTTGGAA GCAAGAATAA TGAAGGCCTG ACGAAACTTC CTTTTACCAT CCCAGAATTG 
GTGCAGGCAT CCCCATGCCG AAGTTCAGAT GGAATCCTCT ACATGGGTAA AAAGCAGGAC 
ATCTGGTATG TTATTGACCT CCTGACCGGA GAGAAGCAGC AGACTTTGTC ATCGGCCTTT 
GCAGATAGTC TCTGCCCATC AACCTCTCTT CTGTATCTTG GGCGAACAGA GATGTCCCAC 
TTTGTGTCCA ATGGTGATGG GCTGGTGGTG ACTGTGGACA GTGAATCTGG GGACGTCCTG 
TGGATCCAAA ACTACGCCTC CCCTGTGGTG GCCTTTTATG TCTGGCAGCG GGAGGGTCTG 
AGGAAGGTGA TGCACATCAA TGTCGCTGTG GAGACCCTGC GCTATCTGAC CTTCATGTCT 
GGGGAGGTGG GGCGCATCAC AAAGTGGAAG TACCCGTTCC CCAAGGAGAC AGAGGCCAAG 
AGCAAGCTGA CGCCCACTCT GTATGTTGGG AAATACTCTA CCAGCCTCTA TGCCTCTCCC 
TCAATGGTAC ACGAGGGGGT TGCTGTCGTG CCCCGCGGCA GCACACTTCC TTTGCTGGAA 
GGGCCCCAGA CTGATGGCGT CACCATTGGG GACAAGGGGG AGTGTGTGAT CACGCCCAGC 
ACGGACGTCA AGTTTGATCC CGGACTCAAA AGCAAGAACA AGCTCAACTA CTTGAGGAAT 
TACTGGCTTC TGATAGGACA CCATGAAACC CCACTGTCTG CGTCTACCAA GATGCTGGAG 
AGATTTCCCA ACAATCTACC CAAACATCGG GAAAATGTGA TTCCTGCTGA TTCAGAGAAA 
AAGAGCTTTG AGGAAGTTAT CAACCTGGTT GACCAGACTT CAGAAAACGC ACCTACCACC 
GTGTCTCGGG ATGTGGAGGA GAAGCCCGCC CATGCCCCTG CCCGGCCCGA GGCCCCCGTG 
GACTCCATGC TTAAGGACAT GGCTACCATC ATCCTGAGCA CCTTCCTGCT GATTGGCTGG 
GTGGCCTTCA TCATCACCTA TCCCCTGAGC ATGCATCAGC AGCAGCAGCT CCAGCACCAG 
CAGTTCCAGA AGGAACTGGA GAAGATCCAG CTCCTGCAGC AGCAGCAGCA GCAGCTGCCC 
TTCCACCCAC CTGGAGACAC GGCTCAGGAC GGCGAGCTCC TGGACACGTC TGGCCCGTAC 
TCAGAGAGCT CGGGCACCAG CAGCCCCAGC ACGTCCCCCA GGGCCTCCAA CCACTCGCTC 
TGCTCCGGCA GCTCTGCCTC CAAGGCTGGC AGCAGCCCCT CCCTGGAACA AGACGATGGA 
GATGAGGAAA CCAGCGTGGT GATAGTTGGG AAAATTTCCT TCTGTCCCAA GGATGTCCTG 
GGCCATGGAG CTGAGGGCAC AATTGTGTAC CGGGGCATGT TTGACAACCG CGACGTGGCC 
GTGAAGAGGA TCCTCCCCGA GTGTTTTAGC TTCGCAGACC GTGAGGTCCA GCTGTTGCGA 
GAATCGGATG AGCACCCGAA CGTGATCCGC TACTTCTGCA CGGAGAAGGA CCGGCAATTC 
CAGTACATTG CCATCGAGCT GTGTGCAGCC ACCCTGCAAG AGTATGTGGA GCAGAAGGAC 
TTTGCGCATC TCGGCCTGGA GCCCATCACC TTGCTGCAGC AGACCACCTC GGGCCTGGCC 
CACCTCCACT CCCTCAACAT CGTTCACAGA GACCTAAAGC CACACAACAT CCTCATATCC 
ATGCCCAATG CACACGGCAA GATCAAGGCC ATGATCTCCG ACTTTGGCCT CTGCAAGAAG 
CTGGCAGTGG GCAGACACAG TTTCAGCCGC CGATCTGGGG TGCCTGGCAC AGAAGGCTGG 
ATCGCTCCAG AGATGCTGAG CGAAGACTGT AAGGAGAACC CTACCTACAC GGTGGACATC 
TTTTCTGCAG GCTGCGTCTT TTACTACGTA ATCTCTGAGG GCAGCCACCC TTTTGGCAAG 
TCCCTGCAGC GGCAGGCCAA CATCCTCCTG GGTGCCTGCA GCCTTGACTG CTTGCACCCA 
GAGAAGCACG AAGACGTCAT TGCACGTGAA TTGATAGAGA AGATGATTGC GATGGATCCT 
CAGAAACGCC CCTCAGCGAA GCATGTGCTC AAACACCCGT TCTTCTGGAG CCTAGAGAAG 
CAGCTCCAGT TCTTCCAGGA CGTGAGCGAC AGAATAGAAA AGGAATCCCT GGATGGCCCG 
ATCGTGAAGC AGTTAGAGAG AGGCGGGAGA GCCGTGGTGA AGATGGACTG GCGGGAGAAC 
ATCACTGTCC CCCTCCAGAC AGACCTGCGT AAATTCAGGA CCTATAAAGG TGGTTCTGTC 
AGAGATCTCC TCCGAGCCAT GAGAAATAAG AAGCACCACT ACCGGGAGCT GCCTGCAGAG 
GTGCGGGAGA CGCTGGGGTC CCTCCCCGAC GACTTCGTGT GCTACTTCAC ATCTCGCTTC 
CCCCACCTCC TCGCACACAC CTACCGGGCC ATGGAGCTGT GCAGCCACGA GAGACTCTTC 
CAGCCCTACT ACTTCCACGA GCCCCCAGAG CCCCAGCCCC CAGTGACTCC AGACGCCCTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016879837.1
CDS	1357..4299
Translation	MQQGVRTLTRELASATCPAGFLVLGGCRLPSVHRDFLEQVELWELHSWKIPHGFEIFGSTSTVTLPETLLFVSTLDGSLHAVSKRTGSIKWTLKEDPVLQVPTHVEEPAFLPDPNDGSLYTLGSKNNEGLTKLPFTIPELVQASPCRSSDGILYMGKKQDIWYVIDLLTGEKQQTLSSAFADSLCPSTSLLYLGRTEMSHFVSNGDGLVVTVDSESGDVLWIQNYASPVVAFYVWQREGLRKVMHINVAVETLRYLTFMSGEVGRITKWKYPFPKETEAKSKLTPTLYVGKYSTSLYASPSMVHEGVAVVPRGSTLPLLEGPQTDGVTIGDKGECVITPSTDVKFDPGLKSKNKLNYLRNYWLLIGHHETPLSASTKMLERFPNNLPKHRENVIPADSEKKSFEEVINLVDQTSENAPTTVSRDVEEKPAHAPARPEAPVDSMLKDMATIILSTFLLIGWVAFIITYPLSMHQQQQLQHQQFQKELEKIQLLQQQQQQLPFHPPGDTAQDGELLDTSGPYSESSGTSSPSTSPRASNHSLCSGSSASKAGSSPSLEQDDGDEETSVVIVGKISFCPKDVLGHGAEGTIVYRGMFDNRDVAVKRILPECFSFADREVQLLRESDEHPNVIRYFCTEKDRQFQYIAIELCAATLQEYVEQKDFAHLGLEPITLLQQTTSGLAHLHSLNIVHRDLKPHNILISMPNAHGKIKAMISDFGLCKKLAVGRHSFSRRSGVPGTEGWIAPEMLSEDCKENPTYTVDIFSAGCVFYYVISEGSHPFGKSLQRQANILLGACSLDCLHPEKHEDVIARELIEKMIAMDPQKRPSAKHVLKHPFFWSLEKQLQFFQDVSDRIEKESLDGPIVKQLERGGRAVVKMDWRENITVPLQTDLRKFRTYKGGSVRDLLRAMRNKKHHYRELPAEVRETLGSLPDDFVCYFTSRFPHLLAHTYRAMELCSHERLFQPYYFHEPPEPQPPVTPDAL

Target ORF information:

RefSeq Version	XM_017024348.1
Organism	Homo sapiens(human)
Definition	Homo sapiens endoplasmic reticulum to nucleus signaling 1 (ERN1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017024348.1

ORF Insert Sequence:

ATGCAACAGG GAGTAAGGAC TCTCACCCGA GAGCTGGCTT CTGCCACTTG CCCTGCCGGT 
TTCTTGGTAC TGGGAGGCTG CAGGCTTCCT TCTGTGCACC GAGACTTCCT TGAGCAAGTG 
GAGCTGTGGG AATTGCACTC TTGGAAGATC CCGCATGGGT TCGAGATTTT TGGAAGTACC 
AGCACAGTGA CGCTTCCTGA AACCTTGTTG TTTGTGTCAA CGCTGGATGG AAGTTTGCAT 
GCTGTCAGCA AGAGGACAGG CTCAATCAAA TGGACTTTAA AAGAAGATCC AGTCCTGCAG 
GTCCCAACAC ATGTGGAAGA GCCTGCCTTT CTCCCAGATC CTAATGATGG CAGCCTGTAT 
ACGCTTGGAA GCAAGAATAA TGAAGGCCTG ACGAAACTTC CTTTTACCAT CCCAGAATTG 
GTGCAGGCAT CCCCATGCCG AAGTTCAGAT GGAATCCTCT ACATGGGTAA AAAGCAGGAC 
ATCTGGTATG TTATTGACCT CCTGACCGGA GAGAAGCAGC AGACTTTGTC ATCGGCCTTT 
GCAGATAGTC TCTGCCCATC AACCTCTCTT CTGTATCTTG GGCGAACAGA GATGTCCCAC 
TTTGTGTCCA ATGGTGATGG GCTGGTGGTG ACTGTGGACA GTGAATCTGG GGACGTCCTG 
TGGATCCAAA ACTACGCCTC CCCTGTGGTG GCCTTTTATG TCTGGCAGCG GGAGGGTCTG 
AGGAAGGTGA TGCACATCAA TGTCGCTGTG GAGACCCTGC GCTATCTGAC CTTCATGTCT 
GGGGAGGTGG GGCGCATCAC AAAGTGGAAG TACCCGTTCC CCAAGGAGAC AGAGGCCAAG 
AGCAAGCTGA CGCCCACTCT GTATGTTGGG AAATACTCTA CCAGCCTCTA TGCCTCTCCC 
TCAATGGTAC ACGAGGGGGT TGCTGTCGTG CCCCGCGGCA GCACACTTCC TTTGCTGGAA 
GGGCCCCAGA CTGATGGCGT CACCATTGGG GACAAGGGGG AGTGTGTGAT CACGCCCAGC 
ACGGACGTCA AGTTTGATCC CGGACTCAAA AGCAAGAACA AGCTCAACTA CTTGAGGAAT 
TACTGGCTTC TGATAGGACA CCATGAAACC CCACTGTCTG CGTCTACCAA GATGCTGGAG 
AGATTTCCCA ACAATCTACC CAAACATCGG GAAAATGTGA TTCCTGCTGA TTCAGAGAAA 
AAGAGCTTTG AGGAAGTTAT CAACCTGGTT GACCAGACTT CAGAAAACGC ACCTACCACC 
GTGTCTCGGG ATGTGGAGGA GAAGCCCGCC CATGCCCCTG CCCGGCCCGA GGCCCCCGTG 
GACTCCATGC TTAAGGACAT GGCTACCATC ATCCTGAGCA CCTTCCTGCT GATTGGCTGG 
GTGGCCTTCA TCATCACCTA TCCCCTGAGC ATGCATCAGC AGCAGCAGCT CCAGCACCAG 
CAGTTCCAGA AGGAACTGGA GAAGATCCAG CTCCTGCAGC AGCAGCAGCA GCAGCTGCCC 
TTCCACCCAC CTGGAGACAC GGCTCAGGAC GGCGAGCTCC TGGACACGTC TGGCCCGTAC 
TCAGAGAGCT CGGGCACCAG CAGCCCCAGC ACGTCCCCCA GGGCCTCCAA CCACTCGCTC 
TGCTCCGGCA GCTCTGCCTC CAAGGCTGGC AGCAGCCCCT CCCTGGAACA AGACGATGGA 
GATGAGGAAA CCAGCGTGGT GATAGTTGGG AAAATTTCCT TCTGTCCCAA GGATGTCCTG 
GGCCATGGAG CTGAGGGCAC AATTGTGTAC CGGGGCATGT TTGACAACCG CGACGTGGCC 
GTGAAGAGGA TCCTCCCCGA GTGTTTTAGC TTCGCAGACC GTGAGGTCCA GCTGTTGCGA 
GAATCGGATG AGCACCCGAA CGTGATCCGC TACTTCTGCA CGGAGAAGGA CCGGCAATTC 
CAGTACATTG CCATCGAGCT GTGTGCAGCC ACCCTGCAAG AGTATGTGGA GCAGAAGGAC 
TTTGCGCATC TCGGCCTGGA GCCCATCACC TTGCTGCAGC AGACCACCTC GGGCCTGGCC 
CACCTCCACT CCCTCAACAT CGTTCACAGA GACCTAAAGC CACACAACAT CCTCATATCC 
ATGCCCAATG CACACGGCAA GATCAAGGCC ATGATCTCCG ACTTTGGCCT CTGCAAGAAG 
CTGGCAGTGG GCAGACACAG TTTCAGCCGC CGATCTGGGG TGCCTGGCAC AGAAGGCTGG 
ATCGCTCCAG AGATGCTGAG CGAAGACTGT AAGGAGAACC CTACCTACAC GGTGGACATC 
TTTTCTGCAG GCTGCGTCTT TTACTACGTA ATCTCTGAGG GCAGCCACCC TTTTGGCAAG 
TCCCTGCAGC GGCAGGCCAA CATCCTCCTG GGTGCCTGCA GCCTTGACTG CTTGCACCCA 
GAGAAGCACG AAGACGTCAT TGCACGTGAA TTGATAGAGA AGATGATTGC GATGGATCCT 
CAGAAACGCC CCTCAGCGAA GCATGTGCTC AAACACCCGT TCTTCTGGAG CCTAGAGAAG 
CAGCTCCAGT TCTTCCAGGA CGTGAGCGAC AGAATAGAAA AGGAATCCCT GGATGGCCCG 
ATCGTGAAGC AGTTAGAGAG AGGCGGGAGA GCCGTGGTGA AGATGGACTG GCGGGAGAAC 
ATCACTGTCC CCCTCCAGAC AGACCTGCGT AAATTCAGGA CCTATAAAGG TGGTTCTGTC 
AGAGATCTCC TCCGAGCCAT GAGAAATAAG AAGCACCACT ACCGGGAGCT GCCTGCAGAG 
GTGCGGGAGA CGCTGGGGTC CCTCCCCGAC GACTTCGTGT GCTACTTCAC ATCTCGCTTC 
CCCCACCTCC TCGCACACAC CTACCGGGCC ATGGAGCTGT GCAGCCACGA GAGACTCTTC 
CAGCCCTACT ACTTCCACGA GCCCCCAGAG CCCCAGCCCC CAGTGACTCC AGACGCCCTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ERN1 ( XM_017024348.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016879837.1 Homo sapiens endoplasmic reticulum to nucleus signaling 1 (ERN1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu86687
Clone ID Related Accession (Same CDS sequence)	XM_017024348.1 , XM_017024348.2
Accession Version	XM_017024348.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2943bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase/endoribonuclease IRE1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017024348.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAACAGG GAGTAAGGAC TCTCACCCGA GAGCTGGCTT CTGCCACTTG CCCTGCCGGT 
TTCTTGGTAC TGGGAGGCTG CAGGCTTCCT TCTGTGCACC GAGACTTCCT TGAGCAAGTG 
GAGCTGTGGG AATTGCACTC TTGGAAGATC CCGCATGGGT TCGAGATTTT TGGAAGTACC 
AGCACAGTGA CGCTTCCTGA AACCTTGTTG TTTGTGTCAA CGCTGGATGG AAGTTTGCAT 
GCTGTCAGCA AGAGGACAGG CTCAATCAAA TGGACTTTAA AAGAAGATCC AGTCCTGCAG 
GTCCCAACAC ATGTGGAAGA GCCTGCCTTT CTCCCAGATC CTAATGATGG CAGCCTGTAT 
ACGCTTGGAA GCAAGAATAA TGAAGGCCTG ACGAAACTTC CTTTTACCAT CCCAGAATTG 
GTGCAGGCAT CCCCATGCCG AAGTTCAGAT GGAATCCTCT ACATGGGTAA AAAGCAGGAC 
ATCTGGTATG TTATTGACCT CCTGACCGGA GAGAAGCAGC AGACTTTGTC ATCGGCCTTT 
GCAGATAGTC TCTGCCCATC AACCTCTCTT CTGTATCTTG GGCGAACAGA GATGTCCCAC 
TTTGTGTCCA ATGGTGATGG GCTGGTGGTG ACTGTGGACA GTGAATCTGG GGACGTCCTG 
TGGATCCAAA ACTACGCCTC CCCTGTGGTG GCCTTTTATG TCTGGCAGCG GGAGGGTCTG 
AGGAAGGTGA TGCACATCAA TGTCGCTGTG GAGACCCTGC GCTATCTGAC CTTCATGTCT 
GGGGAGGTGG GGCGCATCAC AAAGTGGAAG TACCCGTTCC CCAAGGAGAC AGAGGCCAAG 
AGCAAGCTGA CGCCCACTCT GTATGTTGGG AAATACTCTA CCAGCCTCTA TGCCTCTCCC 
TCAATGGTAC ACGAGGGGGT TGCTGTCGTG CCCCGCGGCA GCACACTTCC TTTGCTGGAA 
GGGCCCCAGA CTGATGGCGT CACCATTGGG GACAAGGGGG AGTGTGTGAT CACGCCCAGC 
ACGGACGTCA AGTTTGATCC CGGACTCAAA AGCAAGAACA AGCTCAACTA CTTGAGGAAT 
TACTGGCTTC TGATAGGACA CCATGAAACC CCACTGTCTG CGTCTACCAA GATGCTGGAG 
AGATTTCCCA ACAATCTACC CAAACATCGG GAAAATGTGA TTCCTGCTGA TTCAGAGAAA 
AAGAGCTTTG AGGAAGTTAT CAACCTGGTT GACCAGACTT CAGAAAACGC ACCTACCACC 
GTGTCTCGGG ATGTGGAGGA GAAGCCCGCC CATGCCCCTG CCCGGCCCGA GGCCCCCGTG 
GACTCCATGC TTAAGGACAT GGCTACCATC ATCCTGAGCA CCTTCCTGCT GATTGGCTGG 
GTGGCCTTCA TCATCACCTA TCCCCTGAGC ATGCATCAGC AGCAGCAGCT CCAGCACCAG 
CAGTTCCAGA AGGAACTGGA GAAGATCCAG CTCCTGCAGC AGCAGCAGCA GCAGCTGCCC 
TTCCACCCAC CTGGAGACAC GGCTCAGGAC GGCGAGCTCC TGGACACGTC TGGCCCGTAC 
TCAGAGAGCT CGGGCACCAG CAGCCCCAGC ACGTCCCCCA GGGCCTCCAA CCACTCGCTC 
TGCTCCGGCA GCTCTGCCTC CAAGGCTGGC AGCAGCCCCT CCCTGGAACA AGACGATGGA 
GATGAGGAAA CCAGCGTGGT GATAGTTGGG AAAATTTCCT TCTGTCCCAA GGATGTCCTG 
GGCCATGGAG CTGAGGGCAC AATTGTGTAC CGGGGCATGT TTGACAACCG CGACGTGGCC 
GTGAAGAGGA TCCTCCCCGA GTGTTTTAGC TTCGCAGACC GTGAGGTCCA GCTGTTGCGA 
GAATCGGATG AGCACCCGAA CGTGATCCGC TACTTCTGCA CGGAGAAGGA CCGGCAATTC 
CAGTACATTG CCATCGAGCT GTGTGCAGCC ACCCTGCAAG AGTATGTGGA GCAGAAGGAC 
TTTGCGCATC TCGGCCTGGA GCCCATCACC TTGCTGCAGC AGACCACCTC GGGCCTGGCC 
CACCTCCACT CCCTCAACAT CGTTCACAGA GACCTAAAGC CACACAACAT CCTCATATCC 
ATGCCCAATG CACACGGCAA GATCAAGGCC ATGATCTCCG ACTTTGGCCT CTGCAAGAAG 
CTGGCAGTGG GCAGACACAG TTTCAGCCGC CGATCTGGGG TGCCTGGCAC AGAAGGCTGG 
ATCGCTCCAG AGATGCTGAG CGAAGACTGT AAGGAGAACC CTACCTACAC GGTGGACATC 
TTTTCTGCAG GCTGCGTCTT TTACTACGTA ATCTCTGAGG GCAGCCACCC TTTTGGCAAG 
TCCCTGCAGC GGCAGGCCAA CATCCTCCTG GGTGCCTGCA GCCTTGACTG CTTGCACCCA 
GAGAAGCACG AAGACGTCAT TGCACGTGAA TTGATAGAGA AGATGATTGC GATGGATCCT 
CAGAAACGCC CCTCAGCGAA GCATGTGCTC AAACACCCGT TCTTCTGGAG CCTAGAGAAG 
CAGCTCCAGT TCTTCCAGGA CGTGAGCGAC AGAATAGAAA AGGAATCCCT GGATGGCCCG 
ATCGTGAAGC AGTTAGAGAG AGGCGGGAGA GCCGTGGTGA AGATGGACTG GCGGGAGAAC 
ATCACTGTCC CCCTCCAGAC AGACCTGCGT AAATTCAGGA CCTATAAAGG TGGTTCTGTC 
AGAGATCTCC TCCGAGCCAT GAGAAATAAG AAGCACCACT ACCGGGAGCT GCCTGCAGAG 
GTGCGGGAGA CGCTGGGGTC CCTCCCCGAC GACTTCGTGT GCTACTTCAC ATCTCGCTTC 
CCCCACCTCC TCGCACACAC CTACCGGGCC ATGGAGCTGT GCAGCCACGA GAGACTCTTC 
CAGCCCTACT ACTTCCACGA GCCCCCAGAG CCCCAGCCCC CAGTGACTCC AGACGCCCTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016879837.1
CDS	3007..5949
Translation	MQQGVRTLTRELASATCPAGFLVLGGCRLPSVHRDFLEQVELWELHSWKIPHGFEIFGSTSTVTLPETLLFVSTLDGSLHAVSKRTGSIKWTLKEDPVLQVPTHVEEPAFLPDPNDGSLYTLGSKNNEGLTKLPFTIPELVQASPCRSSDGILYMGKKQDIWYVIDLLTGEKQQTLSSAFADSLCPSTSLLYLGRTEMSHFVSNGDGLVVTVDSESGDVLWIQNYASPVVAFYVWQREGLRKVMHINVAVETLRYLTFMSGEVGRITKWKYPFPKETEAKSKLTPTLYVGKYSTSLYASPSMVHEGVAVVPRGSTLPLLEGPQTDGVTIGDKGECVITPSTDVKFDPGLKSKNKLNYLRNYWLLIGHHETPLSASTKMLERFPNNLPKHRENVIPADSEKKSFEEVINLVDQTSENAPTTVSRDVEEKPAHAPARPEAPVDSMLKDMATIILSTFLLIGWVAFIITYPLSMHQQQQLQHQQFQKELEKIQLLQQQQQQLPFHPPGDTAQDGELLDTSGPYSESSGTSSPSTSPRASNHSLCSGSSASKAGSSPSLEQDDGDEETSVVIVGKISFCPKDVLGHGAEGTIVYRGMFDNRDVAVKRILPECFSFADREVQLLRESDEHPNVIRYFCTEKDRQFQYIAIELCAATLQEYVEQKDFAHLGLEPITLLQQTTSGLAHLHSLNIVHRDLKPHNILISMPNAHGKIKAMISDFGLCKKLAVGRHSFSRRSGVPGTEGWIAPEMLSEDCKENPTYTVDIFSAGCVFYYVISEGSHPFGKSLQRQANILLGACSLDCLHPEKHEDVIARELIEKMIAMDPQKRPSAKHVLKHPFFWSLEKQLQFFQDVSDRIEKESLDGPIVKQLERGGRAVVKMDWRENITVPLQTDLRKFRTYKGGSVRDLLRAMRNKKHHYRELPAEVRETLGSLPDDFVCYFTSRFPHLLAHTYRAMELCSHERLFQPYYFHEPPEPQPPVTPDAL

Target ORF information:

RefSeq Version	XM_017024348.2
Organism	Homo sapiens(human)
Definition	Homo sapiens endoplasmic reticulum to nucleus signaling 1 (ERN1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017024348.2

ORF Insert Sequence:

ATGCAACAGG GAGTAAGGAC TCTCACCCGA GAGCTGGCTT CTGCCACTTG CCCTGCCGGT 
TTCTTGGTAC TGGGAGGCTG CAGGCTTCCT TCTGTGCACC GAGACTTCCT TGAGCAAGTG 
GAGCTGTGGG AATTGCACTC TTGGAAGATC CCGCATGGGT TCGAGATTTT TGGAAGTACC 
AGCACAGTGA CGCTTCCTGA AACCTTGTTG TTTGTGTCAA CGCTGGATGG AAGTTTGCAT 
GCTGTCAGCA AGAGGACAGG CTCAATCAAA TGGACTTTAA AAGAAGATCC AGTCCTGCAG 
GTCCCAACAC ATGTGGAAGA GCCTGCCTTT CTCCCAGATC CTAATGATGG CAGCCTGTAT 
ACGCTTGGAA GCAAGAATAA TGAAGGCCTG ACGAAACTTC CTTTTACCAT CCCAGAATTG 
GTGCAGGCAT CCCCATGCCG AAGTTCAGAT GGAATCCTCT ACATGGGTAA AAAGCAGGAC 
ATCTGGTATG TTATTGACCT CCTGACCGGA GAGAAGCAGC AGACTTTGTC ATCGGCCTTT 
GCAGATAGTC TCTGCCCATC AACCTCTCTT CTGTATCTTG GGCGAACAGA GATGTCCCAC 
TTTGTGTCCA ATGGTGATGG GCTGGTGGTG ACTGTGGACA GTGAATCTGG GGACGTCCTG 
TGGATCCAAA ACTACGCCTC CCCTGTGGTG GCCTTTTATG TCTGGCAGCG GGAGGGTCTG 
AGGAAGGTGA TGCACATCAA TGTCGCTGTG GAGACCCTGC GCTATCTGAC CTTCATGTCT 
GGGGAGGTGG GGCGCATCAC AAAGTGGAAG TACCCGTTCC CCAAGGAGAC AGAGGCCAAG 
AGCAAGCTGA CGCCCACTCT GTATGTTGGG AAATACTCTA CCAGCCTCTA TGCCTCTCCC 
TCAATGGTAC ACGAGGGGGT TGCTGTCGTG CCCCGCGGCA GCACACTTCC TTTGCTGGAA 
GGGCCCCAGA CTGATGGCGT CACCATTGGG GACAAGGGGG AGTGTGTGAT CACGCCCAGC 
ACGGACGTCA AGTTTGATCC CGGACTCAAA AGCAAGAACA AGCTCAACTA CTTGAGGAAT 
TACTGGCTTC TGATAGGACA CCATGAAACC CCACTGTCTG CGTCTACCAA GATGCTGGAG 
AGATTTCCCA ACAATCTACC CAAACATCGG GAAAATGTGA TTCCTGCTGA TTCAGAGAAA 
AAGAGCTTTG AGGAAGTTAT CAACCTGGTT GACCAGACTT CAGAAAACGC ACCTACCACC 
GTGTCTCGGG ATGTGGAGGA GAAGCCCGCC CATGCCCCTG CCCGGCCCGA GGCCCCCGTG 
GACTCCATGC TTAAGGACAT GGCTACCATC ATCCTGAGCA CCTTCCTGCT GATTGGCTGG 
GTGGCCTTCA TCATCACCTA TCCCCTGAGC ATGCATCAGC AGCAGCAGCT CCAGCACCAG 
CAGTTCCAGA AGGAACTGGA GAAGATCCAG CTCCTGCAGC AGCAGCAGCA GCAGCTGCCC 
TTCCACCCAC CTGGAGACAC GGCTCAGGAC GGCGAGCTCC TGGACACGTC TGGCCCGTAC 
TCAGAGAGCT CGGGCACCAG CAGCCCCAGC ACGTCCCCCA GGGCCTCCAA CCACTCGCTC 
TGCTCCGGCA GCTCTGCCTC CAAGGCTGGC AGCAGCCCCT CCCTGGAACA AGACGATGGA 
GATGAGGAAA CCAGCGTGGT GATAGTTGGG AAAATTTCCT TCTGTCCCAA GGATGTCCTG 
GGCCATGGAG CTGAGGGCAC AATTGTGTAC CGGGGCATGT TTGACAACCG CGACGTGGCC 
GTGAAGAGGA TCCTCCCCGA GTGTTTTAGC TTCGCAGACC GTGAGGTCCA GCTGTTGCGA 
GAATCGGATG AGCACCCGAA CGTGATCCGC TACTTCTGCA CGGAGAAGGA CCGGCAATTC 
CAGTACATTG CCATCGAGCT GTGTGCAGCC ACCCTGCAAG AGTATGTGGA GCAGAAGGAC 
TTTGCGCATC TCGGCCTGGA GCCCATCACC TTGCTGCAGC AGACCACCTC GGGCCTGGCC 
CACCTCCACT CCCTCAACAT CGTTCACAGA GACCTAAAGC CACACAACAT CCTCATATCC 
ATGCCCAATG CACACGGCAA GATCAAGGCC ATGATCTCCG ACTTTGGCCT CTGCAAGAAG 
CTGGCAGTGG GCAGACACAG TTTCAGCCGC CGATCTGGGG TGCCTGGCAC AGAAGGCTGG 
ATCGCTCCAG AGATGCTGAG CGAAGACTGT AAGGAGAACC CTACCTACAC GGTGGACATC 
TTTTCTGCAG GCTGCGTCTT TTACTACGTA ATCTCTGAGG GCAGCCACCC TTTTGGCAAG 
TCCCTGCAGC GGCAGGCCAA CATCCTCCTG GGTGCCTGCA GCCTTGACTG CTTGCACCCA 
GAGAAGCACG AAGACGTCAT TGCACGTGAA TTGATAGAGA AGATGATTGC GATGGATCCT 
CAGAAACGCC CCTCAGCGAA GCATGTGCTC AAACACCCGT TCTTCTGGAG CCTAGAGAAG 
CAGCTCCAGT TCTTCCAGGA CGTGAGCGAC AGAATAGAAA AGGAATCCCT GGATGGCCCG 
ATCGTGAAGC AGTTAGAGAG AGGCGGGAGA GCCGTGGTGA AGATGGACTG GCGGGAGAAC 
ATCACTGTCC CCCTCCAGAC AGACCTGCGT AAATTCAGGA CCTATAAAGG TGGTTCTGTC 
AGAGATCTCC TCCGAGCCAT GAGAAATAAG AAGCACCACT ACCGGGAGCT GCCTGCAGAG 
GTGCGGGAGA CGCTGGGGTC CCTCCCCGAC GACTTCGTGT GCTACTTCAC ATCTCGCTTC 
CCCCACCTCC TCGCACACAC CTACCGGGCC ATGGAGCTGT GCAGCCACGA GAGACTCTTC 
CAGCCCTACT ACTTCCACGA GCCCCCAGAG CCCCAGCCCC CAGTGACTCC AGACGCCCTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ERN1 ( XM_017024347.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016879836.1 Homo sapiens endoplasmic reticulum to nucleus signaling 1 (ERN1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu86686
Clone ID Related Accession (Same CDS sequence)	XM_017024347.1 , XM_017024347.2
Accession Version	XM_017024347.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3045bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase/endoribonuclease IRE1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAACAGG GAGTAAGGAC TCTCACCCGA GAGCTGGCTT CTGCCACTTG CCCTGCCGGT 
TTCTTGGTAC TGGGAGGCTG CAGGCTTCCT TCTGTGCACC GAGACTTCCT TGAGCAAGTG 
GAGCTGTGGG AATTGCACTC TTGGAAGATC CCGCATGGGT TCGAGATTTT TGGAAGTACC 
AGCACAGTGA CGCTTCCTGA AACCTTGTTG TTTGTGTCAA CGCTGGATGG AAGTTTGCAT 
GCTGTCAGCA AGAGGACAGG CTCAATCAAA TGGACTTTAA AAGAAGATCC AGTCCTGCAG 
GTCCCAACAC ATGTGGAAGA GCCTGCCTTT CTCCCAGATC CTAATGATGG CAGCCTGTAT 
ACGCTTGGAA GCAAGAATAA TGAAGGCCTG ACGAAACTTC CTTTTACCAT CCCAGAATTG 
GTGCAGGCAT CCCCATGCCG AAGTTCAGAT GGAATCCTCT ACATGGGTAA AAAGCAGGAC 
ATCTGGTATG TTATTGACCT CCTGACCGGA GAGAAGCAGC AGACTTTGTC ATCGGCCTTT 
GCAGATAGTC TCTGCCCATC AACCTCTCTT CTGTATCTTG GGCGAACAGA ATACACCATC 
ACCATGTACG ACACCAAAAC CCGAGAGCTC CGGTGGAATG CCACCTACTT TGACTATGCG 
GCCTCACTGC CTGAGGACGA CGTGGACTAC AAGATGTCCC ACTTTGTGTC CAATGGTGAT 
GGGCTGGTGG TGACTGTGGA CAGTGAATCT GGGGACGTCC TGTGGATCCA AAACTACGCC 
TCCCCTGTGG TGGCCTTTTA TGTCTGGCAG CGGGAGGGTC TGAGGAAGGT GATGCACATC 
AATGTCGCTG TGGAGACCCT GCGCTATCTG ACCTTCATGT CTGGGGAGGT GGGGCGCATC 
ACAAAGTGGA AGTACCCGTT CCCCAAGGAG ACAGAGGCCA AGAGCAAGCT GACGCCCACT 
CTGTATGTTG GGAAATACTC TACCAGCCTC TATGCCTCTC CCTCAATGGT ACACGAGGGG 
GTTGCTGTCG TGCCCCGCGG CAGCACACTT CCTTTGCTGG AAGGGCCCCA GACTGATGGC 
GTCACCATTG GGGACAAGGG GGAGTGTGTG ATCACGCCCA GCACGGACGT CAAGTTTGAT 
CCCGGACTCA AAAGCAAGAA CAAGCTCAAC TACTTGAGGA ATTACTGGCT TCTGATAGGA 
CACCATGAAA CCCCACTGTC TGCGTCTACC AAGATGCTGG AGAGATTTCC CAACAATCTA 
CCCAAACATC GGGAAAATGT GATTCCTGCT GATTCAGAGA AAAAGAGCTT TGAGGAAGTT 
ATCAACCTGG TTGACCAGAC TTCAGAAAAC GCACCTACCA CCGTGTCTCG GGATGTGGAG 
GAGAAGCCCG CCCATGCCCC TGCCCGGCCC GAGGCCCCCG TGGACTCCAT GCTTAAGGAC 
ATGGCTACCA TCATCCTGAG CACCTTCCTG CTGATTGGCT GGGTGGCCTT CATCATCACC 
TATCCCCTGA GCATGCATCA GCAGCAGCAG CTCCAGCACC AGCAGTTCCA GAAGGAACTG 
GAGAAGATCC AGCTCCTGCA GCAGCAGCAG CAGCAGCTGC CCTTCCACCC ACCTGGAGAC 
ACGGCTCAGG ACGGCGAGCT CCTGGACACG TCTGGCCCGT ACTCAGAGAG CTCGGGCACC 
AGCAGCCCCA GCACGTCCCC CAGGGCCTCC AACCACTCGC TCTGCTCCGG CAGCTCTGCC 
TCCAAGGCTG GCAGCAGCCC CTCCCTGGAA CAAGACGATG GAGATGAGGA AACCAGCGTG 
GTGATAGTTG GGAAAATTTC CTTCTGTCCC AAGGATGTCC TGGGCCATGG AGCTGAGGGC 
ACAATTGTGT ACCGGGGCAT GTTTGACAAC CGCGACGTGG CCGTGAAGAG GATCCTCCCC 
GAGTGTTTTA GCTTCGCAGA CCGTGAGGTC CAGCTGTTGC GAGAATCGGA TGAGCACCCG 
AACGTGATCC GCTACTTCTG CACGGAGAAG GACCGGCAAT TCCAGTACAT TGCCATCGAG 
CTGTGTGCAG CCACCCTGCA AGAGTATGTG GAGCAGAAGG ACTTTGCGCA TCTCGGCCTG 
GAGCCCATCA CCTTGCTGCA GCAGACCACC TCGGGCCTGG CCCACCTCCA CTCCCTCAAC 
ATCGTTCACA GAGACCTAAA GCCACACAAC ATCCTCATAT CCATGCCCAA TGCACACGGC 
AAGATCAAGG CCATGATCTC CGACTTTGGC CTCTGCAAGA AGCTGGCAGT GGGCAGACAC 
AGTTTCAGCC GCCGATCTGG GGTGCCTGGC ACAGAAGGCT GGATCGCTCC AGAGATGCTG 
AGCGAAGACT GTAAGGAGAA CCCTACCTAC ACGGTGGACA TCTTTTCTGC AGGCTGCGTC 
TTTTACTACG TAATCTCTGA GGGCAGCCAC CCTTTTGGCA AGTCCCTGCA GCGGCAGGCC 
AACATCCTCC TGGGTGCCTG CAGCCTTGAC TGCTTGCACC CAGAGAAGCA CGAAGACGTC 
ATTGCACGTG AATTGATAGA GAAGATGATT GCGATGGATC CTCAGAAACG CCCCTCAGCG 
AAGCATGTGC TCAAACACCC GTTCTTCTGG AGCCTAGAGA AGCAGCTCCA GTTCTTCCAG 
GACGTGAGCG ACAGAATAGA AAAGGAATCC CTGGATGGCC CGATCGTGAA GCAGTTAGAG 
AGAGGCGGGA GAGCCGTGGT GAAGATGGAC TGGCGGGAGA ACATCACTGT CCCCCTCCAG 
ACAGACCTGC GTAAATTCAG GACCTATAAA GGTGGTTCTG TCAGAGATCT CCTCCGAGCC 
ATGAGAAATA AGAAGCACCA CTACCGGGAG CTGCCTGCAG AGGTGCGGGA GACGCTGGGG 
TCCCTCCCCG ACGACTTCGT GTGCTACTTC ACATCTCGCT TCCCCCACCT CCTCGCACAC 
ACCTACCGGG CCATGGAGCT GTGCAGCCAC GAGAGACTCT TCCAGCCCTA CTACTTCCAC 
GAGCCCCCAG AGCCCCAGCC CCCAGTGACT CCAGACGCCC TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016879836.1
CDS	1357..4401
Translation	MQQGVRTLTRELASATCPAGFLVLGGCRLPSVHRDFLEQVELWELHSWKIPHGFEIFGSTSTVTLPETLLFVSTLDGSLHAVSKRTGSIKWTLKEDPVLQVPTHVEEPAFLPDPNDGSLYTLGSKNNEGLTKLPFTIPELVQASPCRSSDGILYMGKKQDIWYVIDLLTGEKQQTLSSAFADSLCPSTSLLYLGRTEYTITMYDTKTRELRWNATYFDYAASLPEDDVDYKMSHFVSNGDGLVVTVDSESGDVLWIQNYASPVVAFYVWQREGLRKVMHINVAVETLRYLTFMSGEVGRITKWKYPFPKETEAKSKLTPTLYVGKYSTSLYASPSMVHEGVAVVPRGSTLPLLEGPQTDGVTIGDKGECVITPSTDVKFDPGLKSKNKLNYLRNYWLLIGHHETPLSASTKMLERFPNNLPKHRENVIPADSEKKSFEEVINLVDQTSENAPTTVSRDVEEKPAHAPARPEAPVDSMLKDMATIILSTFLLIGWVAFIITYPLSMHQQQQLQHQQFQKELEKIQLLQQQQQQLPFHPPGDTAQDGELLDTSGPYSESSGTSSPSTSPRASNHSLCSGSSASKAGSSPSLEQDDGDEETSVVIVGKISFCPKDVLGHGAEGTIVYRGMFDNRDVAVKRILPECFSFADREVQLLRESDEHPNVIRYFCTEKDRQFQYIAIELCAATLQEYVEQKDFAHLGLEPITLLQQTTSGLAHLHSLNIVHRDLKPHNILISMPNAHGKIKAMISDFGLCKKLAVGRHSFSRRSGVPGTEGWIAPEMLSEDCKENPTYTVDIFSAGCVFYYVISEGSHPFGKSLQRQANILLGACSLDCLHPEKHEDVIARELIEKMIAMDPQKRPSAKHVLKHPFFWSLEKQLQFFQDVSDRIEKESLDGPIVKQLERGGRAVVKMDWRENITVPLQTDLRKFRTYKGGSVRDLLRAMRNKKHHYRELPAEVRETLGSLPDDFVCYFTSRFPHLLAHTYRAMELCSHERLFQPYYFHEPPEPQPPVTPDAL

Target ORF information:

RefSeq Version	XM_017024347.1
Organism	Homo sapiens(human)
Definition	Homo sapiens endoplasmic reticulum to nucleus signaling 1 (ERN1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017024347.1

ORF Insert Sequence:

ATGCAACAGG GAGTAAGGAC TCTCACCCGA GAGCTGGCTT CTGCCACTTG CCCTGCCGGT 
TTCTTGGTAC TGGGAGGCTG CAGGCTTCCT TCTGTGCACC GAGACTTCCT TGAGCAAGTG 
GAGCTGTGGG AATTGCACTC TTGGAAGATC CCGCATGGGT TCGAGATTTT TGGAAGTACC 
AGCACAGTGA CGCTTCCTGA AACCTTGTTG TTTGTGTCAA CGCTGGATGG AAGTTTGCAT 
GCTGTCAGCA AGAGGACAGG CTCAATCAAA TGGACTTTAA AAGAAGATCC AGTCCTGCAG 
GTCCCAACAC ATGTGGAAGA GCCTGCCTTT CTCCCAGATC CTAATGATGG CAGCCTGTAT 
ACGCTTGGAA GCAAGAATAA TGAAGGCCTG ACGAAACTTC CTTTTACCAT CCCAGAATTG 
GTGCAGGCAT CCCCATGCCG AAGTTCAGAT GGAATCCTCT ACATGGGTAA AAAGCAGGAC 
ATCTGGTATG TTATTGACCT CCTGACCGGA GAGAAGCAGC AGACTTTGTC ATCGGCCTTT 
GCAGATAGTC TCTGCCCATC AACCTCTCTT CTGTATCTTG GGCGAACAGA ATACACCATC 
ACCATGTACG ACACCAAAAC CCGAGAGCTC CGGTGGAATG CCACCTACTT TGACTATGCG 
GCCTCACTGC CTGAGGACGA CGTGGACTAC AAGATGTCCC ACTTTGTGTC CAATGGTGAT 
GGGCTGGTGG TGACTGTGGA CAGTGAATCT GGGGACGTCC TGTGGATCCA AAACTACGCC 
TCCCCTGTGG TGGCCTTTTA TGTCTGGCAG CGGGAGGGTC TGAGGAAGGT GATGCACATC 
AATGTCGCTG TGGAGACCCT GCGCTATCTG ACCTTCATGT CTGGGGAGGT GGGGCGCATC 
ACAAAGTGGA AGTACCCGTT CCCCAAGGAG ACAGAGGCCA AGAGCAAGCT GACGCCCACT 
CTGTATGTTG GGAAATACTC TACCAGCCTC TATGCCTCTC CCTCAATGGT ACACGAGGGG 
GTTGCTGTCG TGCCCCGCGG CAGCACACTT CCTTTGCTGG AAGGGCCCCA GACTGATGGC 
GTCACCATTG GGGACAAGGG GGAGTGTGTG ATCACGCCCA GCACGGACGT CAAGTTTGAT 
CCCGGACTCA AAAGCAAGAA CAAGCTCAAC TACTTGAGGA ATTACTGGCT TCTGATAGGA 
CACCATGAAA CCCCACTGTC TGCGTCTACC AAGATGCTGG AGAGATTTCC CAACAATCTA 
CCCAAACATC GGGAAAATGT GATTCCTGCT GATTCAGAGA AAAAGAGCTT TGAGGAAGTT 
ATCAACCTGG TTGACCAGAC TTCAGAAAAC GCACCTACCA CCGTGTCTCG GGATGTGGAG 
GAGAAGCCCG CCCATGCCCC TGCCCGGCCC GAGGCCCCCG TGGACTCCAT GCTTAAGGAC 
ATGGCTACCA TCATCCTGAG CACCTTCCTG CTGATTGGCT GGGTGGCCTT CATCATCACC 
TATCCCCTGA GCATGCATCA GCAGCAGCAG CTCCAGCACC AGCAGTTCCA GAAGGAACTG 
GAGAAGATCC AGCTCCTGCA GCAGCAGCAG CAGCAGCTGC CCTTCCACCC ACCTGGAGAC 
ACGGCTCAGG ACGGCGAGCT CCTGGACACG TCTGGCCCGT ACTCAGAGAG CTCGGGCACC 
AGCAGCCCCA GCACGTCCCC CAGGGCCTCC AACCACTCGC TCTGCTCCGG CAGCTCTGCC 
TCCAAGGCTG GCAGCAGCCC CTCCCTGGAA CAAGACGATG GAGATGAGGA AACCAGCGTG 
GTGATAGTTG GGAAAATTTC CTTCTGTCCC AAGGATGTCC TGGGCCATGG AGCTGAGGGC 
ACAATTGTGT ACCGGGGCAT GTTTGACAAC CGCGACGTGG CCGTGAAGAG GATCCTCCCC 
GAGTGTTTTA GCTTCGCAGA CCGTGAGGTC CAGCTGTTGC GAGAATCGGA TGAGCACCCG 
AACGTGATCC GCTACTTCTG CACGGAGAAG GACCGGCAAT TCCAGTACAT TGCCATCGAG 
CTGTGTGCAG CCACCCTGCA AGAGTATGTG GAGCAGAAGG ACTTTGCGCA TCTCGGCCTG 
GAGCCCATCA CCTTGCTGCA GCAGACCACC TCGGGCCTGG CCCACCTCCA CTCCCTCAAC 
ATCGTTCACA GAGACCTAAA GCCACACAAC ATCCTCATAT CCATGCCCAA TGCACACGGC 
AAGATCAAGG CCATGATCTC CGACTTTGGC CTCTGCAAGA AGCTGGCAGT GGGCAGACAC 
AGTTTCAGCC GCCGATCTGG GGTGCCTGGC ACAGAAGGCT GGATCGCTCC AGAGATGCTG 
AGCGAAGACT GTAAGGAGAA CCCTACCTAC ACGGTGGACA TCTTTTCTGC AGGCTGCGTC 
TTTTACTACG TAATCTCTGA GGGCAGCCAC CCTTTTGGCA AGTCCCTGCA GCGGCAGGCC 
AACATCCTCC TGGGTGCCTG CAGCCTTGAC TGCTTGCACC CAGAGAAGCA CGAAGACGTC 
ATTGCACGTG AATTGATAGA GAAGATGATT GCGATGGATC CTCAGAAACG CCCCTCAGCG 
AAGCATGTGC TCAAACACCC GTTCTTCTGG AGCCTAGAGA AGCAGCTCCA GTTCTTCCAG 
GACGTGAGCG ACAGAATAGA AAAGGAATCC CTGGATGGCC CGATCGTGAA GCAGTTAGAG 
AGAGGCGGGA GAGCCGTGGT GAAGATGGAC TGGCGGGAGA ACATCACTGT CCCCCTCCAG 
ACAGACCTGC GTAAATTCAG GACCTATAAA GGTGGTTCTG TCAGAGATCT CCTCCGAGCC 
ATGAGAAATA AGAAGCACCA CTACCGGGAG CTGCCTGCAG AGGTGCGGGA GACGCTGGGG 
TCCCTCCCCG ACGACTTCGT GTGCTACTTC ACATCTCGCT TCCCCCACCT CCTCGCACAC 
ACCTACCGGG CCATGGAGCT GTGCAGCCAC GAGAGACTCT TCCAGCCCTA CTACTTCCAC 
GAGCCCCCAG AGCCCCAGCC CCCAGTGACT CCAGACGCCC TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ERN1 ( XM_017024347.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016879836.1 Homo sapiens endoplasmic reticulum to nucleus signaling 1 (ERN1), transcript variant X1, mRNA.

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CloneID	OHu86686
Clone ID Related Accession (Same CDS sequence)	XM_017024347.1 , XM_017024347.2
Accession Version	XM_017024347.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3045bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase/endoribonuclease IRE1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017024347.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAACAGG GAGTAAGGAC TCTCACCCGA GAGCTGGCTT CTGCCACTTG CCCTGCCGGT 
TTCTTGGTAC TGGGAGGCTG CAGGCTTCCT TCTGTGCACC GAGACTTCCT TGAGCAAGTG 
GAGCTGTGGG AATTGCACTC TTGGAAGATC CCGCATGGGT TCGAGATTTT TGGAAGTACC 
AGCACAGTGA CGCTTCCTGA AACCTTGTTG TTTGTGTCAA CGCTGGATGG AAGTTTGCAT 
GCTGTCAGCA AGAGGACAGG CTCAATCAAA TGGACTTTAA AAGAAGATCC AGTCCTGCAG 
GTCCCAACAC ATGTGGAAGA GCCTGCCTTT CTCCCAGATC CTAATGATGG CAGCCTGTAT 
ACGCTTGGAA GCAAGAATAA TGAAGGCCTG ACGAAACTTC CTTTTACCAT CCCAGAATTG 
GTGCAGGCAT CCCCATGCCG AAGTTCAGAT GGAATCCTCT ACATGGGTAA AAAGCAGGAC 
ATCTGGTATG TTATTGACCT CCTGACCGGA GAGAAGCAGC AGACTTTGTC ATCGGCCTTT 
GCAGATAGTC TCTGCCCATC AACCTCTCTT CTGTATCTTG GGCGAACAGA ATACACCATC 
ACCATGTACG ACACCAAAAC CCGAGAGCTC CGGTGGAATG CCACCTACTT TGACTATGCG 
GCCTCACTGC CTGAGGACGA CGTGGACTAC AAGATGTCCC ACTTTGTGTC CAATGGTGAT 
GGGCTGGTGG TGACTGTGGA CAGTGAATCT GGGGACGTCC TGTGGATCCA AAACTACGCC 
TCCCCTGTGG TGGCCTTTTA TGTCTGGCAG CGGGAGGGTC TGAGGAAGGT GATGCACATC 
AATGTCGCTG TGGAGACCCT GCGCTATCTG ACCTTCATGT CTGGGGAGGT GGGGCGCATC 
ACAAAGTGGA AGTACCCGTT CCCCAAGGAG ACAGAGGCCA AGAGCAAGCT GACGCCCACT 
CTGTATGTTG GGAAATACTC TACCAGCCTC TATGCCTCTC CCTCAATGGT ACACGAGGGG 
GTTGCTGTCG TGCCCCGCGG CAGCACACTT CCTTTGCTGG AAGGGCCCCA GACTGATGGC 
GTCACCATTG GGGACAAGGG GGAGTGTGTG ATCACGCCCA GCACGGACGT CAAGTTTGAT 
CCCGGACTCA AAAGCAAGAA CAAGCTCAAC TACTTGAGGA ATTACTGGCT TCTGATAGGA 
CACCATGAAA CCCCACTGTC TGCGTCTACC AAGATGCTGG AGAGATTTCC CAACAATCTA 
CCCAAACATC GGGAAAATGT GATTCCTGCT GATTCAGAGA AAAAGAGCTT TGAGGAAGTT 
ATCAACCTGG TTGACCAGAC TTCAGAAAAC GCACCTACCA CCGTGTCTCG GGATGTGGAG 
GAGAAGCCCG CCCATGCCCC TGCCCGGCCC GAGGCCCCCG TGGACTCCAT GCTTAAGGAC 
ATGGCTACCA TCATCCTGAG CACCTTCCTG CTGATTGGCT GGGTGGCCTT CATCATCACC 
TATCCCCTGA GCATGCATCA GCAGCAGCAG CTCCAGCACC AGCAGTTCCA GAAGGAACTG 
GAGAAGATCC AGCTCCTGCA GCAGCAGCAG CAGCAGCTGC CCTTCCACCC ACCTGGAGAC 
ACGGCTCAGG ACGGCGAGCT CCTGGACACG TCTGGCCCGT ACTCAGAGAG CTCGGGCACC 
AGCAGCCCCA GCACGTCCCC CAGGGCCTCC AACCACTCGC TCTGCTCCGG CAGCTCTGCC 
TCCAAGGCTG GCAGCAGCCC CTCCCTGGAA CAAGACGATG GAGATGAGGA AACCAGCGTG 
GTGATAGTTG GGAAAATTTC CTTCTGTCCC AAGGATGTCC TGGGCCATGG AGCTGAGGGC 
ACAATTGTGT ACCGGGGCAT GTTTGACAAC CGCGACGTGG CCGTGAAGAG GATCCTCCCC 
GAGTGTTTTA GCTTCGCAGA CCGTGAGGTC CAGCTGTTGC GAGAATCGGA TGAGCACCCG 
AACGTGATCC GCTACTTCTG CACGGAGAAG GACCGGCAAT TCCAGTACAT TGCCATCGAG 
CTGTGTGCAG CCACCCTGCA AGAGTATGTG GAGCAGAAGG ACTTTGCGCA TCTCGGCCTG 
GAGCCCATCA CCTTGCTGCA GCAGACCACC TCGGGCCTGG CCCACCTCCA CTCCCTCAAC 
ATCGTTCACA GAGACCTAAA GCCACACAAC ATCCTCATAT CCATGCCCAA TGCACACGGC 
AAGATCAAGG CCATGATCTC CGACTTTGGC CTCTGCAAGA AGCTGGCAGT GGGCAGACAC 
AGTTTCAGCC GCCGATCTGG GGTGCCTGGC ACAGAAGGCT GGATCGCTCC AGAGATGCTG 
AGCGAAGACT GTAAGGAGAA CCCTACCTAC ACGGTGGACA TCTTTTCTGC AGGCTGCGTC 
TTTTACTACG TAATCTCTGA GGGCAGCCAC CCTTTTGGCA AGTCCCTGCA GCGGCAGGCC 
AACATCCTCC TGGGTGCCTG CAGCCTTGAC TGCTTGCACC CAGAGAAGCA CGAAGACGTC 
ATTGCACGTG AATTGATAGA GAAGATGATT GCGATGGATC CTCAGAAACG CCCCTCAGCG 
AAGCATGTGC TCAAACACCC GTTCTTCTGG AGCCTAGAGA AGCAGCTCCA GTTCTTCCAG 
GACGTGAGCG ACAGAATAGA AAAGGAATCC CTGGATGGCC CGATCGTGAA GCAGTTAGAG 
AGAGGCGGGA GAGCCGTGGT GAAGATGGAC TGGCGGGAGA ACATCACTGT CCCCCTCCAG 
ACAGACCTGC GTAAATTCAG GACCTATAAA GGTGGTTCTG TCAGAGATCT CCTCCGAGCC 
ATGAGAAATA AGAAGCACCA CTACCGGGAG CTGCCTGCAG AGGTGCGGGA GACGCTGGGG 
TCCCTCCCCG ACGACTTCGT GTGCTACTTC ACATCTCGCT TCCCCCACCT CCTCGCACAC 
ACCTACCGGG CCATGGAGCT GTGCAGCCAC GAGAGACTCT TCCAGCCCTA CTACTTCCAC 
GAGCCCCCAG AGCCCCAGCC CCCAGTGACT CCAGACGCCC TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016879836.1
CDS	3007..6051
Translation	MQQGVRTLTRELASATCPAGFLVLGGCRLPSVHRDFLEQVELWELHSWKIPHGFEIFGSTSTVTLPETLLFVSTLDGSLHAVSKRTGSIKWTLKEDPVLQVPTHVEEPAFLPDPNDGSLYTLGSKNNEGLTKLPFTIPELVQASPCRSSDGILYMGKKQDIWYVIDLLTGEKQQTLSSAFADSLCPSTSLLYLGRTEYTITMYDTKTRELRWNATYFDYAASLPEDDVDYKMSHFVSNGDGLVVTVDSESGDVLWIQNYASPVVAFYVWQREGLRKVMHINVAVETLRYLTFMSGEVGRITKWKYPFPKETEAKSKLTPTLYVGKYSTSLYASPSMVHEGVAVVPRGSTLPLLEGPQTDGVTIGDKGECVITPSTDVKFDPGLKSKNKLNYLRNYWLLIGHHETPLSASTKMLERFPNNLPKHRENVIPADSEKKSFEEVINLVDQTSENAPTTVSRDVEEKPAHAPARPEAPVDSMLKDMATIILSTFLLIGWVAFIITYPLSMHQQQQLQHQQFQKELEKIQLLQQQQQQLPFHPPGDTAQDGELLDTSGPYSESSGTSSPSTSPRASNHSLCSGSSASKAGSSPSLEQDDGDEETSVVIVGKISFCPKDVLGHGAEGTIVYRGMFDNRDVAVKRILPECFSFADREVQLLRESDEHPNVIRYFCTEKDRQFQYIAIELCAATLQEYVEQKDFAHLGLEPITLLQQTTSGLAHLHSLNIVHRDLKPHNILISMPNAHGKIKAMISDFGLCKKLAVGRHSFSRRSGVPGTEGWIAPEMLSEDCKENPTYTVDIFSAGCVFYYVISEGSHPFGKSLQRQANILLGACSLDCLHPEKHEDVIARELIEKMIAMDPQKRPSAKHVLKHPFFWSLEKQLQFFQDVSDRIEKESLDGPIVKQLERGGRAVVKMDWRENITVPLQTDLRKFRTYKGGSVRDLLRAMRNKKHHYRELPAEVRETLGSLPDDFVCYFTSRFPHLLAHTYRAMELCSHERLFQPYYFHEPPEPQPPVTPDAL

Target ORF information:

RefSeq Version	XM_017024347.2
Organism	Homo sapiens(human)
Definition	Homo sapiens endoplasmic reticulum to nucleus signaling 1 (ERN1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017024347.2

ORF Insert Sequence:

ATGCAACAGG GAGTAAGGAC TCTCACCCGA GAGCTGGCTT CTGCCACTTG CCCTGCCGGT 
TTCTTGGTAC TGGGAGGCTG CAGGCTTCCT TCTGTGCACC GAGACTTCCT TGAGCAAGTG 
GAGCTGTGGG AATTGCACTC TTGGAAGATC CCGCATGGGT TCGAGATTTT TGGAAGTACC 
AGCACAGTGA CGCTTCCTGA AACCTTGTTG TTTGTGTCAA CGCTGGATGG AAGTTTGCAT 
GCTGTCAGCA AGAGGACAGG CTCAATCAAA TGGACTTTAA AAGAAGATCC AGTCCTGCAG 
GTCCCAACAC ATGTGGAAGA GCCTGCCTTT CTCCCAGATC CTAATGATGG CAGCCTGTAT 
ACGCTTGGAA GCAAGAATAA TGAAGGCCTG ACGAAACTTC CTTTTACCAT CCCAGAATTG 
GTGCAGGCAT CCCCATGCCG AAGTTCAGAT GGAATCCTCT ACATGGGTAA AAAGCAGGAC 
ATCTGGTATG TTATTGACCT CCTGACCGGA GAGAAGCAGC AGACTTTGTC ATCGGCCTTT 
GCAGATAGTC TCTGCCCATC AACCTCTCTT CTGTATCTTG GGCGAACAGA ATACACCATC 
ACCATGTACG ACACCAAAAC CCGAGAGCTC CGGTGGAATG CCACCTACTT TGACTATGCG 
GCCTCACTGC CTGAGGACGA CGTGGACTAC AAGATGTCCC ACTTTGTGTC CAATGGTGAT 
GGGCTGGTGG TGACTGTGGA CAGTGAATCT GGGGACGTCC TGTGGATCCA AAACTACGCC 
TCCCCTGTGG TGGCCTTTTA TGTCTGGCAG CGGGAGGGTC TGAGGAAGGT GATGCACATC 
AATGTCGCTG TGGAGACCCT GCGCTATCTG ACCTTCATGT CTGGGGAGGT GGGGCGCATC 
ACAAAGTGGA AGTACCCGTT CCCCAAGGAG ACAGAGGCCA AGAGCAAGCT GACGCCCACT 
CTGTATGTTG GGAAATACTC TACCAGCCTC TATGCCTCTC CCTCAATGGT ACACGAGGGG 
GTTGCTGTCG TGCCCCGCGG CAGCACACTT CCTTTGCTGG AAGGGCCCCA GACTGATGGC 
GTCACCATTG GGGACAAGGG GGAGTGTGTG ATCACGCCCA GCACGGACGT CAAGTTTGAT 
CCCGGACTCA AAAGCAAGAA CAAGCTCAAC TACTTGAGGA ATTACTGGCT TCTGATAGGA 
CACCATGAAA CCCCACTGTC TGCGTCTACC AAGATGCTGG AGAGATTTCC CAACAATCTA 
CCCAAACATC GGGAAAATGT GATTCCTGCT GATTCAGAGA AAAAGAGCTT TGAGGAAGTT 
ATCAACCTGG TTGACCAGAC TTCAGAAAAC GCACCTACCA CCGTGTCTCG GGATGTGGAG 
GAGAAGCCCG CCCATGCCCC TGCCCGGCCC GAGGCCCCCG TGGACTCCAT GCTTAAGGAC 
ATGGCTACCA TCATCCTGAG CACCTTCCTG CTGATTGGCT GGGTGGCCTT CATCATCACC 
TATCCCCTGA GCATGCATCA GCAGCAGCAG CTCCAGCACC AGCAGTTCCA GAAGGAACTG 
GAGAAGATCC AGCTCCTGCA GCAGCAGCAG CAGCAGCTGC CCTTCCACCC ACCTGGAGAC 
ACGGCTCAGG ACGGCGAGCT CCTGGACACG TCTGGCCCGT ACTCAGAGAG CTCGGGCACC 
AGCAGCCCCA GCACGTCCCC CAGGGCCTCC AACCACTCGC TCTGCTCCGG CAGCTCTGCC 
TCCAAGGCTG GCAGCAGCCC CTCCCTGGAA CAAGACGATG GAGATGAGGA AACCAGCGTG 
GTGATAGTTG GGAAAATTTC CTTCTGTCCC AAGGATGTCC TGGGCCATGG AGCTGAGGGC 
ACAATTGTGT ACCGGGGCAT GTTTGACAAC CGCGACGTGG CCGTGAAGAG GATCCTCCCC 
GAGTGTTTTA GCTTCGCAGA CCGTGAGGTC CAGCTGTTGC GAGAATCGGA TGAGCACCCG 
AACGTGATCC GCTACTTCTG CACGGAGAAG GACCGGCAAT TCCAGTACAT TGCCATCGAG 
CTGTGTGCAG CCACCCTGCA AGAGTATGTG GAGCAGAAGG ACTTTGCGCA TCTCGGCCTG 
GAGCCCATCA CCTTGCTGCA GCAGACCACC TCGGGCCTGG CCCACCTCCA CTCCCTCAAC 
ATCGTTCACA GAGACCTAAA GCCACACAAC ATCCTCATAT CCATGCCCAA TGCACACGGC 
AAGATCAAGG CCATGATCTC CGACTTTGGC CTCTGCAAGA AGCTGGCAGT GGGCAGACAC 
AGTTTCAGCC GCCGATCTGG GGTGCCTGGC ACAGAAGGCT GGATCGCTCC AGAGATGCTG 
AGCGAAGACT GTAAGGAGAA CCCTACCTAC ACGGTGGACA TCTTTTCTGC AGGCTGCGTC 
TTTTACTACG TAATCTCTGA GGGCAGCCAC CCTTTTGGCA AGTCCCTGCA GCGGCAGGCC 
AACATCCTCC TGGGTGCCTG CAGCCTTGAC TGCTTGCACC CAGAGAAGCA CGAAGACGTC 
ATTGCACGTG AATTGATAGA GAAGATGATT GCGATGGATC CTCAGAAACG CCCCTCAGCG 
AAGCATGTGC TCAAACACCC GTTCTTCTGG AGCCTAGAGA AGCAGCTCCA GTTCTTCCAG 
GACGTGAGCG ACAGAATAGA AAAGGAATCC CTGGATGGCC CGATCGTGAA GCAGTTAGAG 
AGAGGCGGGA GAGCCGTGGT GAAGATGGAC TGGCGGGAGA ACATCACTGT CCCCCTCCAG 
ACAGACCTGC GTAAATTCAG GACCTATAAA GGTGGTTCTG TCAGAGATCT CCTCCGAGCC 
ATGAGAAATA AGAAGCACCA CTACCGGGAG CTGCCTGCAG AGGTGCGGGA GACGCTGGGG 
TCCCTCCCCG ACGACTTCGT GTGCTACTTC ACATCTCGCT TCCCCCACCT CCTCGCACAC 
ACCTACCGGG CCATGGAGCT GTGCAGCCAC GAGAGACTCT TCCAGCCCTA CTACTTCCAC 
GAGCCCCCAG AGCCCCAGCC CCCAGTGACT CCAGACGCCC TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A J-Protein Co-chaperone Recruits BiP to Monomerize IRE1 and Repress the Unfolded Protein Response.
Cell171(7)1625-1637.e13(2017 Dec)
Amin-Wetzel N,Saunders RA,Kamphuis MJ,Rato C,Preissler S,Harding HP,Ron D

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ERN1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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