usp-48 cDNA ORF clone, Caenorhabditis elegans

Gene
Clones
gRNAs

Summary

Gene Symbol	usp-48
Entrez Gene ID	172782
Full Name	Ubiquitin carboxyl-terminal hydrolase
Gene Type	protein-coding
Organism	Caenorhabditis elegans

mRNA and Protein(s)

mRNA	Protein	Name
NM_060123.5	NP_492524.2	Ubiquitin carboxyl-terminal hydrolase
NM_001375102.1	NP_001362163.1	Ubiquitin Specific Protease
NM_001375101.1	NP_001361851.1	USP domain-containing protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.

Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following usp-48 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the usp-48 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa335401	NM_060123.5 Latest version!	Caenorhabditis elegans Ubiquitin carboxyl-terminal hydrolase (usp-48), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OCa850863	NM_001375101.1 Latest version!	Caenorhabditis elegans USP domain-containing protein (usp-48), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OCa850864	NM_001375102.1 Latest version!	Caenorhabditis elegans Ubiquitin Specific Protease (usp-48), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

usp-48 ( NM_060123.5 ) cDNA ORF clone, Caenorhabditis elegans -> NP_492524.2 Caenorhabditis elegans Ubiquitin carboxyl-terminal hydrolase (usp-48), partial mRNA.

Price & Availability↑

CloneID	OCa335401
Clone ID Related Accession (Same CDS sequence)	NM_060123.5
Accession Version	NM_060123.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3789bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Ubiquitin carboxyl-terminal hydrolase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003279). On Jun 26, 2012 this sequence version replaced NM_060123.4. COMPLETENESS: incomplete on both ends.

ATGACAAAGG AGACTGACAA AAAGCCAAAA GAACGTCCGA ATCAAAGAAA AGTTGCATTT 
CGAAATGAAG CAATAAATGC ATTAGTAAAT ACAGATGAGG ACCAAGTCAC ATTTTCGAAA 
GCAATGGAAG TTGCCAAATT AGATTGTCAA AAGTGTCTTC TACACGATAT GCATAGTTCA 
AAGTCATCGA ATTGTCGCGA TAATCCATTT TGTATACATC GATTAGGACT CGAGAAGTTT 
GAAAAGTTGA TAACTCAGGA GCAGGAAACT AAAGAAGAAG CGAAAAAAGA TCAAAAGCGG 
AGAGATCTGA ATGATCAACC AGCCGGGCTT ATCAATGGAG GAAACTTTTG CTATGTGAAT 
AGTTTTCTTC AAGTCTGGTT CAACGTTCCT GAGTTCCGGC AGTTGATCTA TGATTTCCGA 
CCATCGGAGA ACTTTGTTCC GCCAGAAGCT CCAAGAATGA ATGTTCAAGC AACTATGCTC 
GCGCTTCAAG ACATTTTTTA TACACTACAG ACAACTCCTT TCAACGAGAC CGACAAAACT 
TCGAATCTTG GGAAATTACT TCGATTGAAT TCAGAACAAC AAGATTCTCA GGAATTCGGA 
TTAAAATTCT TCAATGCACT CGAACGATGT CTTCCAGATC ATCCGAATGG AAAAGAAACT 
TTGAAAAGAT TAAAAGATCT TTTCACTGGA GAGACTTGTA CTCGAATTGT ATGCAAATGT 
GGACAACGAT CTGAACGGGA AGAAACAGCA ATCTCATTGA CTTTGAATAT TGAAGGTTAT 
TGCACACTTC TAGATGCTCT TGATGCTTAC TTTGGAGAAG AACATCTGGA CGACTTCAAA 
TGCTCAAAAT GCAATAAAAC TGGAGATGTT TCGAAGCAAT CCGATTATGT TAAGCTACCT 
CCTGTCATTG TGATTCAGCT CAATCGATAC AAATACACAT CCAAAGGGCG TCAAAAGTTA 
AAAACACCAA TGGCATATCC AAGAGAAATT CCAGCAAAAG CATTTCAAAG AACGAATAAT 
TCAATTCCTC CGCCTGCTGA GATGTATGAT CTTTTCGCAG TTACCATCCA CGAAGGCAAT 
AACGCTGAAT GCGGGCATTA CTATGATCTT ATCAAATCAC CGCTCAATCA GAAATGGTAT 
CGGTACAATG ACGAGACAGT CGAAGCCATC CCAAAGCCAC CTGGAACTGA GAAACCCACC 
ACAGCAAAAA CTGAAAAATC ACGGAAAAAA GATAAAGAAA AATATCCAAC GGATCAAAAA 
GCATGTTATG GACTTCTTTA CCGGAGACGT GATGCATTTA AACCACTTCC ACATCCAAAA 
CTTCCTCCAG AAGAATTGAT TATTGACTCG AAAACTGAGA TCGAAGAATT ATTCGAAGGA 
TTGACGAAAA AGAAAATTGA GAAGAGTGAA AAGAGATTGT ATGATCTTGA GAGACGGATT 
AACAAGGTGA AGATTTCGTA TGGAAAGCTG GAAACACATA GCGATAAATA TAAAGAAGCA 
AATGAAGTTG TCTTTTTGCC AACAACTCTT CTGCAGGATG TATTAGCTCA GGAATATGAA 
GTTGCAAAAG GAGAAAAGAA GAAAAAGAAG AAAGAAGCAT CGGAAAATGA GGAGAAAAAG 
AAAAATGAAG AGGATGAAGC ATTGTCCGCC GCGATCGCTG CATCTGAAGC CGATCAGAGA 
GATAAAGCTT CGTCTGAACC TTCAACTTCT GCTGCTGCAA CAGAAGCAGG AGATGATGAG 
GAGTTGAGAG CAGAAAGTGA AACACCGAAT CCTGAAAATG CTGAATCAAC ACAAGTTGCT 
ATCATGGAAA CAGATGAGAT CATGGATACA ACTCCCACAA AAGATATTGA TATTCTCGCG 
AAGGCAATGG AAGATAATGC ACTCCCAACC GTTGAGGTGC CACAACCAGA ACTCAAAAAA 
CGAACTCGTC AGCAGAATGG AGAAGTCAAA TACGTGTATT CTCAACGAAC TCCTAGAAAA 
AGCCATAATG GGACGAATGG AACTAACTCG AGTCCCCAGA AACAACCGGT TTCATCACGA 
GTTGCGGCTC TGCTGAGTTC TCATGAAATT CCAACATGTG GTCACGGAAA GATGTCAATC 
GATCCAATTC TTTACGGAGA TGTCAAAGCA GTATCACGGG CTCCAGCAAT CGCCTTGCTC 
CGTGAGTATG ACTTTCGAGT GAAAATCGTG TATGATAATG GAGAGAACGT TTTCCCGGAA 
AATGAAAAGG AACGAGATGT TTTTATTTTC ACAGCAGAAG ACATTTGTAT GGAATGTGTG 
AGGGAAATGC GTGAGGAAGG AAATTTTAAC AATCAACTGG AAGACGATGA AAAGCTTGTT 
CGACGTATTC TGAAAGAAGA AAAGCAACGG TGTTCTGTGA AATGCCCTTC CGAACGGCCC 
GATGGATACC TTTACGTAGC TAAATTTGCA CTTTCCAACT TCAAGAAGTC AGCCATGTCA 
GCTCGCGAGA ACCGACTTGC TCAATCACAT AACAAACAAG GAACACTTCA TTTTGACTCT 
CATCCGATGC AAAAATCGAA TTCTGGCTAC CTAACGTTGT CGTTGAAAAG GACTCGAGGA 
AAACCTCGCA AATCATTGTC GGAGATTCCT GAAAAGATGC AGAAGCTTGA TGAAATTGGA 
TCGAAAGAAT TGCCAGATGA GATTATTGCT GATGAAGAAG AAATTTCGGA AAATATGGGA 
TCCGATATAC CAACAAAACC GGTGGAGTCG ATAAATCCAG ATGCGCTTGT TCCCTTCGAG 
AAGATTGAAT TTAATTCAGA GCTTCGTTGT TCACACGGAG GAATCAATTT TAATCAATTC 
AGACTATCTG TTTCTCCCGA AGAGTGGGCT CATTTAAAAG TCTATTTCGA TGAATGTTAC 
GAGGTGAAAT GTTCGGACGA CGTGTGTGAT CAGTGTCGAC AAATGGAGGT TGACGCACAA 
AACGGATCCG AGAACATGCG TGGGTTGGTC AGAGAGATGC GCAAACGAAT CAGTGACACA 
TTAAAAACCG TTGAATCAAG AGCTGAATCT AAAGAAGATG GTGCAGACAT AAAGTATGGA 
ATCTGTAGTG TCTTTATAGA TAAGCTTAGA AAGCTGACGA GTCGACAATC TACGAGTCCG 
CCATCTATCT GCCAAGAGTG CCTTCTTTGT CCACATCAGC AGCCGTTTAA AGGATTTTTG 
AATGAAGATA ATCACAAAGA TTCACACGTC GTCGGTCTTA CCGAAGAGGA ATGGAACACG 
TTTCTTACAG AAATAAGGAA ACTTGAAGAA GCTGGTGATG ATCAATCGAT TGCGGTAGAT 
CCTTGTCCAA TCCCCATTGA AAATGGTCAA ATTGTGGACA TGTGCGAAAA GTGTTTTGAG 
CAACATATAA AGTTCACTGA AGAACAGAAA TACATGTTTG AAAATGAGAA TATATATGTG 
AAACTTGTGA ATTTGAATGT CGAGGAAGAT ATTGCGAAGG CGAATGGGAA AGCTAGAAGA 
GGACGAGCTA AGAACTTGTA TGCTATAAAG ATGTCCTCGA CGAATAAGCT GATGGAGCTG 
AAAGTTCAAT TGTATGATAA AACACACCAA TTACCAAACG ATCAACTATT GTATCGTACA 
GCCGGCGGTG AACAGTTTGA TGTTAGCAAC AATCAGAAAA CATTGTTCGA TCTTCGTCTT 
TCTCCAAATA ATAACGATAA TCCTCTCATC CTGATTGCTC AACAGTTTTC TCCATCTGCG 
TCTCAAGCTG ATGAAACTGG TGATCGTGCC CCGGAGCGTG GTTTCGTGGA TACAGCGCTC 
GCTCATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_492524.2
CDS	1..3789
Translation	MTKETDKKPKERPNQRKVAFRNEAINALVNTDEDQVTFSKAMEVAKLDCQKCLLHDMHSSKSSNCRDNPFCIHRLGLEKFEKLITQEQETKEEAKKDQKRRDLNDQPAGLINGGNFCYVNSFLQVWFNVPEFRQLIYDFRPSENFVPPEAPRMNVQATMLALQDIFYTLQTTPFNETDKTSNLGKLLRLNSEQQDSQEFGLKFFNALERCLPDHPNGKETLKRLKDLFTGETCTRIVCKCGQRSEREETAISLTLNIEGYCTLLDALDAYFGEEHLDDFKCSKCNKTGDVSKQSDYVKLPPVIVIQLNRYKYTSKGRQKLKTPMAYPREIPAKAFQRTNNSIPPPAEMYDLFAVTIHEGNNAECGHYYDLIKSPLNQKWYRYNDETVEAIPKPPGTEKPTTAKTEKSRKKDKEKYPTDQKACYGLLYRRRDAFKPLPHPKLPPEELIIDSKTEIEELFEGLTKKKIEKSEKRLYDLERRINKVKISYGKLETHSDKYKEANEVVFLPTTLLQDVLAQEYEVAKGEKKKKKKEASENEEKKKNEEDEALSAAIAASEADQRDKASSEPSTSAAATEAGDDEELRAESETPNPENAESTQVAIMETDEIMDTTPTKDIDILAKAMEDNALPTVEVPQPELKKRTRQQNGEVKYVYSQRTPRKSHNGTNGTNSSPQKQPVSSRVAALLSSHEIPTCGHGKMSIDPILYGDVKAVSRAPAIALLREYDFRVKIVYDNGENVFPENEKERDVFIFTAEDICMECVREMREEGNFNNQLEDDEKLVRRILKEEKQRCSVKCPSERPDGYLYVAKFALSNFKKSAMSARENRLAQSHNKQGTLHFDSHPMQKSNSGYLTLSLKRTRGKPRKSLSEIPEKMQKLDEIGSKELPDEIIADEEEISENMGSDIPTKPVESINPDALVPFEKIEFNSELRCSHGGINFNQFRLSVSPEEWAHLKVYFDECYEVKCSDDVCDQCRQMEVDAQNGSENMRGLVREMRKRISDTLKTVESRAESKEDGADIKYGICSVFIDKLRKLTSRQSTSPPSICQECLLCPHQQPFKGFLNEDNHKDSHVVGLTEEEWNTFLTEIRKLEEAGDDQSIAVDPCPIPIENGQIVDMCEKCFEQHIKFTEEQKYMFENENIYVKLVNLNVEEDIAKANGKARRGRAKNLYAIKMSSTNKLMELKVQLYDKTHQLPNDQLLYRTAGGEQFDVSNNQKTLFDLRLSPNNNDNPLILIAQQFSPSASQADETGDRAPERGFVDTALAH

Target ORF information:

RefSeq Version	NM_060123.5
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Ubiquitin carboxyl-terminal hydrolase (usp-48), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_060123.5

ORF Insert Sequence:

ATGACAAAGG AGACTGACAA AAAGCCAAAA GAACGTCCGA ATCAAAGAAA AGTTGCATTT 
CGAAATGAAG CAATAAATGC ATTAGTAAAT ACAGATGAGG ACCAAGTCAC ATTTTCGAAA 
GCAATGGAAG TTGCCAAATT AGATTGTCAA AAGTGTCTTC TACACGATAT GCATAGTTCA 
AAGTCATCGA ATTGTCGCGA TAATCCATTT TGTATACATC GATTAGGACT CGAGAAGTTT 
GAAAAGTTGA TAACTCAGGA GCAGGAAACT AAAGAAGAAG CGAAAAAAGA TCAAAAGCGG 
AGAGATCTGA ATGATCAACC AGCCGGGCTT ATCAATGGAG GAAACTTTTG CTATGTGAAT 
AGTTTTCTTC AAGTCTGGTT CAACGTTCCT GAGTTCCGGC AGTTGATCTA TGATTTCCGA 
CCATCGGAGA ACTTTGTTCC GCCAGAAGCT CCAAGAATGA ATGTTCAAGC AACTATGCTC 
GCGCTTCAAG ACATTTTTTA TACACTACAG ACAACTCCTT TCAACGAGAC CGACAAAACT 
TCGAATCTTG GGAAATTACT TCGATTGAAT TCAGAACAAC AAGATTCTCA GGAATTCGGA 
TTAAAATTCT TCAATGCACT CGAACGATGT CTTCCAGATC ATCCGAATGG AAAAGAAACT 
TTGAAAAGAT TAAAAGATCT TTTCACTGGA GAGACTTGTA CTCGAATTGT ATGCAAATGT 
GGACAACGAT CTGAACGGGA AGAAACAGCA ATCTCATTGA CTTTGAATAT TGAAGGTTAT 
TGCACACTTC TAGATGCTCT TGATGCTTAC TTTGGAGAAG AACATCTGGA CGACTTCAAA 
TGCTCAAAAT GCAATAAAAC TGGAGATGTT TCGAAGCAAT CCGATTATGT TAAGCTACCT 
CCTGTCATTG TGATTCAGCT CAATCGATAC AAATACACAT CCAAAGGGCG TCAAAAGTTA 
AAAACACCAA TGGCATATCC AAGAGAAATT CCAGCAAAAG CATTTCAAAG AACGAATAAT 
TCAATTCCTC CGCCTGCTGA GATGTATGAT CTTTTCGCAG TTACCATCCA CGAAGGCAAT 
AACGCTGAAT GCGGGCATTA CTATGATCTT ATCAAATCAC CGCTCAATCA GAAATGGTAT 
CGGTACAATG ACGAGACAGT CGAAGCCATC CCAAAGCCAC CTGGAACTGA GAAACCCACC 
ACAGCAAAAA CTGAAAAATC ACGGAAAAAA GATAAAGAAA AATATCCAAC GGATCAAAAA 
GCATGTTATG GACTTCTTTA CCGGAGACGT GATGCATTTA AACCACTTCC ACATCCAAAA 
CTTCCTCCAG AAGAATTGAT TATTGACTCG AAAACTGAGA TCGAAGAATT ATTCGAAGGA 
TTGACGAAAA AGAAAATTGA GAAGAGTGAA AAGAGATTGT ATGATCTTGA GAGACGGATT 
AACAAGGTGA AGATTTCGTA TGGAAAGCTG GAAACACATA GCGATAAATA TAAAGAAGCA 
AATGAAGTTG TCTTTTTGCC AACAACTCTT CTGCAGGATG TATTAGCTCA GGAATATGAA 
GTTGCAAAAG GAGAAAAGAA GAAAAAGAAG AAAGAAGCAT CGGAAAATGA GGAGAAAAAG 
AAAAATGAAG AGGATGAAGC ATTGTCCGCC GCGATCGCTG CATCTGAAGC CGATCAGAGA 
GATAAAGCTT CGTCTGAACC TTCAACTTCT GCTGCTGCAA CAGAAGCAGG AGATGATGAG 
GAGTTGAGAG CAGAAAGTGA AACACCGAAT CCTGAAAATG CTGAATCAAC ACAAGTTGCT 
ATCATGGAAA CAGATGAGAT CATGGATACA ACTCCCACAA AAGATATTGA TATTCTCGCG 
AAGGCAATGG AAGATAATGC ACTCCCAACC GTTGAGGTGC CACAACCAGA ACTCAAAAAA 
CGAACTCGTC AGCAGAATGG AGAAGTCAAA TACGTGTATT CTCAACGAAC TCCTAGAAAA 
AGCCATAATG GGACGAATGG AACTAACTCG AGTCCCCAGA AACAACCGGT TTCATCACGA 
GTTGCGGCTC TGCTGAGTTC TCATGAAATT CCAACATGTG GTCACGGAAA GATGTCAATC 
GATCCAATTC TTTACGGAGA TGTCAAAGCA GTATCACGGG CTCCAGCAAT CGCCTTGCTC 
CGTGAGTATG ACTTTCGAGT GAAAATCGTG TATGATAATG GAGAGAACGT TTTCCCGGAA 
AATGAAAAGG AACGAGATGT TTTTATTTTC ACAGCAGAAG ACATTTGTAT GGAATGTGTG 
AGGGAAATGC GTGAGGAAGG AAATTTTAAC AATCAACTGG AAGACGATGA AAAGCTTGTT 
CGACGTATTC TGAAAGAAGA AAAGCAACGG TGTTCTGTGA AATGCCCTTC CGAACGGCCC 
GATGGATACC TTTACGTAGC TAAATTTGCA CTTTCCAACT TCAAGAAGTC AGCCATGTCA 
GCTCGCGAGA ACCGACTTGC TCAATCACAT AACAAACAAG GAACACTTCA TTTTGACTCT 
CATCCGATGC AAAAATCGAA TTCTGGCTAC CTAACGTTGT CGTTGAAAAG GACTCGAGGA 
AAACCTCGCA AATCATTGTC GGAGATTCCT GAAAAGATGC AGAAGCTTGA TGAAATTGGA 
TCGAAAGAAT TGCCAGATGA GATTATTGCT GATGAAGAAG AAATTTCGGA AAATATGGGA 
TCCGATATAC CAACAAAACC GGTGGAGTCG ATAAATCCAG ATGCGCTTGT TCCCTTCGAG 
AAGATTGAAT TTAATTCAGA GCTTCGTTGT TCACACGGAG GAATCAATTT TAATCAATTC 
AGACTATCTG TTTCTCCCGA AGAGTGGGCT CATTTAAAAG TCTATTTCGA TGAATGTTAC 
GAGGTGAAAT GTTCGGACGA CGTGTGTGAT CAGTGTCGAC AAATGGAGGT TGACGCACAA 
AACGGATCCG AGAACATGCG TGGGTTGGTC AGAGAGATGC GCAAACGAAT CAGTGACACA 
TTAAAAACCG TTGAATCAAG AGCTGAATCT AAAGAAGATG GTGCAGACAT AAAGTATGGA 
ATCTGTAGTG TCTTTATAGA TAAGCTTAGA AAGCTGACGA GTCGACAATC TACGAGTCCG 
CCATCTATCT GCCAAGAGTG CCTTCTTTGT CCACATCAGC AGCCGTTTAA AGGATTTTTG 
AATGAAGATA ATCACAAAGA TTCACACGTC GTCGGTCTTA CCGAAGAGGA ATGGAACACG 
TTTCTTACAG AAATAAGGAA ACTTGAAGAA GCTGGTGATG ATCAATCGAT TGCGGTAGAT 
CCTTGTCCAA TCCCCATTGA AAATGGTCAA ATTGTGGACA TGTGCGAAAA GTGTTTTGAG 
CAACATATAA AGTTCACTGA AGAACAGAAA TACATGTTTG AAAATGAGAA TATATATGTG 
AAACTTGTGA ATTTGAATGT CGAGGAAGAT ATTGCGAAGG CGAATGGGAA AGCTAGAAGA 
GGACGAGCTA AGAACTTGTA TGCTATAAAG ATGTCCTCGA CGAATAAGCT GATGGAGCTG 
AAAGTTCAAT TGTATGATAA AACACACCAA TTACCAAACG ATCAACTATT GTATCGTACA 
GCCGGCGGTG AACAGTTTGA TGTTAGCAAC AATCAGAAAA CATTGTTCGA TCTTCGTCTT 
TCTCCAAATA ATAACGATAA TCCTCTCATC CTGATTGCTC AACAGTTTTC TCCATCTGCG 
TCTCAAGCTG ATGAAACTGG TGATCGTGCC CCGGAGCGTG GTTTCGTGGA TACAGCGCTC 
GCTCATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

usp-48 ( NM_001375101.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001361851.1 Caenorhabditis elegans USP domain-containing protein (usp-48), mRNA.

Price & Availability↑

CloneID	OCa850863
Clone ID Related Accession (Same CDS sequence)	NM_001375101.1
Accession Version	NM_001375101.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3795bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	USP domain-containing protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003279).

ATGACAAAGG AGACTGACAA AAAGCCAAAA GAACGTCCGA ATCAAAGAAA AGTTGCATTT 
CGAAATGAAG CAATAAATGC ATTAGTAAAT ACAGATGAGG ACCAAGTCAC ATTTTCGAAA 
GCAATGGAAG TTGCCAAATT AGATTGTCAA AAGTGTCTTC TACACGATAT GCATAGTTCA 
AAGTCATCGA ATTGTCGCGA TAATCCATTT TGTATACATC GATTAGGACT CGAGAAGTTT 
GAAAAGTTGA TAACTCAGGA GCAGGAAACT AAAGAAGAAG CGAAAAAAGA TCAAAAGCGG 
AGAGATCTGA ATGATCAACC AGCCGGGCTT ATCAATGGAG GAAACTTTTG CTATGTGAAT 
AGTTTTCTTC AAGTCTGGTT CAACGTTCCT GAGTTCCGGC AGTTGATCTA TGATTTCCGA 
CCATCGGAGA ACTTTGTTCC GCCAGAAGCT CCAAGAATGA ATGTTCAAGC AACTATGCTC 
GCGCTTCAAG ACATTTTTTA TACACTACAG ACAACTCCTT TCAACGAGAC CGACAAAACT 
TCGAATCTTG GGAAATTACT TCGATTGAAT TCAGAACAAC AAGATTCTCA GGAATTCGGA 
TTAAAATTCT TCAATGCACT CGAACGATGT CTTCCAGATC ATCCGAATGG AAAAGAAACT 
TTGAAAAGAT TAAAAGATCT TTTCACTGGA GAGACTTGTA CTCGAATTGT ATGCAAATGT 
GGACAACGAT CTGAACGGGA AGAAACAGCA ATCTCATTGA CTTTGAATAT TGAAGGTTAT 
TGCACACTTC TAGATGCTCT TGATGCTTAC TTTGGAGAAG AACATCTGGA CGACTTCAAA 
TGCTCAAAAT GCAATAAAAC TGGAGATGTT TCGAAGCAAT CCGATTATGT TAAGCTACCT 
CCTGTCATTG TGATTCAGCT CAATCGATAC AAATACACAT CCAAAGGGCG TCAAAAGTTA 
AAAACACCAA TGGCATATCC AAGAGAAATT CCAGCAAAAG CATTTCAAAG AACGAATAAT 
TCAATTCCTC CGCCTGCTGA GATGTATGAT CTTTTCGCAG TTACCATCCA CGAAGGCAAT 
AACGCTGAAT GCGGGCATTA CTATGATCTT ATCAAATCAC CGCTCAATCA GAAATGGTAT 
CGGTACAATG ACGAGGCGAT CGAAGCCATC CCAAAGCCAC CTGGAACTGA GAAACCCACC 
ACAGCAAAAA CTGAAAAATC ACGGAAAAAA GATAAAGAAA AATATCCAAC GGATCAAAAA 
GCATGTTATG GACTTCTTTA CCGGAGACGT GATGCATTTA AACCACTTCC ACATCCAAAA 
CTTCCTCCAG AAGAATTGAT TATTGACTCG AAAACTGAGA TCGAAGAATT ATTCGAAGGA 
TTGACGAAAA AGAAAATTGA GAAGAGTGAA AAGAGATTGT ATGATCTTGA GAGACGGATT 
AACAAGGTGA AGATTTCGTA TGGAAAGCTG GAAACACATA GCGATAAATA TAAAGAAGCA 
AATGAAGTTG TCTTTTTGCC AACAACTCTT CTGCAGGATG TATTAGCTCA GGAATATGAA 
GTTGCAAAAG GAGAAAAGAA GAAAAAGAAG AAAGAAGCAT CGGAAAATGA GGAGAAAAAG 
AAAAATGAAG AGGATGAAGC ATTGTCCGCC GCGATCGCTG CATCTGAAGC CGATCAGAGA 
GATAAAGCTT CGTCTGAACC TTCAACTTCT GCTGCTGCAA CAGAAGCAGG AGATGATGAG 
GAGTTGAGAG CAGAAAGTGA AACACCGAAT CCTGAAAATG CTGAATCAAC ACAAGTTGCT 
ATCATGGAAA CAGATGAGAT CATGGATACA ACTCCCACAA AAGATATTGA TATTCTCGCG 
AAGGCAATGG AAGATAATGC ACTCCCAACC GTTGAGGTGC CACAACCAGA ACTCAAAAAA 
CGAACTCGTC AGCAGAATGG AGAAGTCAAA TACGTGTATT CTCAACGAAC TCCTAGAAAA 
AGCCATAATG GGACGAATGG AACTAACTCG AGTCCCCAGA AACAACCGGT TTCATCACGA 
GTTGCGGCTC TGCTGAGTTC TCATGAAATT CCAACATGTG GTCACGGAAA GATGTCAATC 
GATCCAATTC TTTACGGAGA TGTCAAAGCA GTATCACGGG CTCCAGCAAT CGCCTTGCTC 
CGTGAGTATG ACTTTCGAGT GAAAATCGTG TATGATAATG GAGAGAACGT TTTCCCGGAA 
AATGAAAAGG AACGAGATGT TTTTATTTTC ACAGCAGAAG ACATTTGTAT GGAATGTGTG 
AGGGAAATGC GTGAGGAAGG AAATTTTAAC AATCAACTGG AAGACGATGA AAAGCTTGTT 
CGACGTATTC TGAAAGAAGA AAAGCAACGG TGTTCTGTGA AATGCCCTTC CGAACGGCCC 
GATGGATACC TTTACGTAGC TAAATTTGCA CTTTCCAACT TCAAGAAGTC AGCCATGTCA 
GCTCGCGAGA ACCGACTTGC TCAATCACAT AACAAACAAG GAACACTTCA TTTTGACTCT 
CATCCGATGT TTCAGCAAAA ATCGAATTCT GGCTACCTAA CGTTGTCGTT GAAAAGGACT 
CGAGGAAAAC CTCGCAAATC ATTGTCGGAG ATTCCTGAAA AGATGCAGAA GCTTGATGAA 
ATTGGATCGA AAGAATTGCC AGATGAGATT ATTGCTGATG AAGAAGAAAT TTCGGAAAAT 
ATGGGATCCG ATATACCAAC AAAACCGGTG GAGTCGATAA ATCCAGATGC GCTTGTTCCC 
TTCGAGAAGA TTGAATTTAA TTCAGAGCTT CGTTGTTCAC ACGGAGGAAT CAATTTTAAT 
CAATTCAGAC TATCTGTTTC TCCCGAAGAG TGGGCTCATT TAAAAGTCTA TTTCGATGAA 
TGTTACGAGG TGAAATGTTC GGACGACGTG TGTGATCAGT GTCGACAAAT GGAGGTTGAC 
GCACAAAACG GATCCGAGAA CATGCGTGGG TTGGTCAGAG AGATGCGCAA ACGAATCAGT 
GACACATTAA AAACCGTTGA ATCAAGAGCT GAATCTAAAG AAGATGGTGC AGACATAAAG 
TATGGAATCT GTAGTGTCTT TATAGATAAG CTTAGAAAGC TGACGAGTCG ACAATCTACG 
AGTCCGCCAT CTATCTGCCA AGAGTGCCTT CTTTGTCCAC ATCAGCAGCC GTTTAAAGGA 
TTTTTGAATG AAGATAATCA CAAAGATTCA CACGTCGTCG GTCTTACCGA AGAGGAATGG 
AACACGTTTC TTACAGAAAT AAGGAAACTT GAAGAAGCTG GTGATGATCA ATCGATTGCG 
GTAGATCCTT GTCCAATCCC CATTGAAAAT GGTCAAATTG TGGACATGTG CGAAAAGTGT 
TTTGAGCAAC ATATAAAGTT CACTGAAGAA CAGAAATACA TGTTTGAAAA TGAGAATATA 
TATGTGAAAC TTGTGAATTT GAATGTCGAG GAAGATATTG CGAAGGCGAA TGGGAAAGCT 
AGAAGAGGAC GAGCTAAGAA CTTGTATGCT ATAAAGATGT CCTCGACGAA TAAGCTGATG 
GAGCTGAAAG TTCAATTGTA TGATAAAACA CACCAATTAC CAAACGATCA ACTATTGTAT 
CGTACAGCCG GCGGTGAACA GTTTGATGTT AGCAACAATC AGAAAACATT GTTCGATCTT 
CGTCTTTCTC CAAATAATAA CGATAATCCT CTCATCCTGA TTGCTCAACA GTTTTCTCCA 
TCTGCGTCTC AAGCTGATGA AACTGGTGAT CGTGCCCCGG AGCGTGGTTT CGTGGATACA 
GCGCTCGCTC ATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001361851.1
CDS	4..3798
Translation	MTKETDKKPKERPNQRKVAFRNEAINALVNTDEDQVTFSKAMEVAKLDCQKCLLHDMHSSKSSNCRDNPFCIHRLGLEKFEKLITQEQETKEEAKKDQKRRDLNDQPAGLINGGNFCYVNSFLQVWFNVPEFRQLIYDFRPSENFVPPEAPRMNVQATMLALQDIFYTLQTTPFNETDKTSNLGKLLRLNSEQQDSQEFGLKFFNALERCLPDHPNGKETLKRLKDLFTGETCTRIVCKCGQRSEREETAISLTLNIEGYCTLLDALDAYFGEEHLDDFKCSKCNKTGDVSKQSDYVKLPPVIVIQLNRYKYTSKGRQKLKTPMAYPREIPAKAFQRTNNSIPPPAEMYDLFAVTIHEGNNAECGHYYDLIKSPLNQKWYRYNDEAIEAIPKPPGTEKPTTAKTEKSRKKDKEKYPTDQKACYGLLYRRRDAFKPLPHPKLPPEELIIDSKTEIEELFEGLTKKKIEKSEKRLYDLERRINKVKISYGKLETHSDKYKEANEVVFLPTTLLQDVLAQEYEVAKGEKKKKKKEASENEEKKKNEEDEALSAAIAASEADQRDKASSEPSTSAAATEAGDDEELRAESETPNPENAESTQVAIMETDEIMDTTPTKDIDILAKAMEDNALPTVEVPQPELKKRTRQQNGEVKYVYSQRTPRKSHNGTNGTNSSPQKQPVSSRVAALLSSHEIPTCGHGKMSIDPILYGDVKAVSRAPAIALLREYDFRVKIVYDNGENVFPENEKERDVFIFTAEDICMECVREMREEGNFNNQLEDDEKLVRRILKEEKQRCSVKCPSERPDGYLYVAKFALSNFKKSAMSARENRLAQSHNKQGTLHFDSHPMFQQKSNSGYLTLSLKRTRGKPRKSLSEIPEKMQKLDEIGSKELPDEIIADEEEISENMGSDIPTKPVESINPDALVPFEKIEFNSELRCSHGGINFNQFRLSVSPEEWAHLKVYFDECYEVKCSDDVCDQCRQMEVDAQNGSENMRGLVREMRKRISDTLKTVESRAESKEDGADIKYGICSVFIDKLRKLTSRQSTSPPSICQECLLCPHQQPFKGFLNEDNHKDSHVVGLTEEEWNTFLTEIRKLEEAGDDQSIAVDPCPIPIENGQIVDMCEKCFEQHIKFTEEQKYMFENENIYVKLVNLNVEEDIAKANGKARRGRAKNLYAIKMSSTNKLMELKVQLYDKTHQLPNDQLLYRTAGGEQFDVSNNQKTLFDLRLSPNNNDNPLILIAQQFSPSASQADETGDRAPERGFVDTALAH

Target ORF information:

RefSeq Version	NM_001375101.1
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans USP domain-containing protein (usp-48), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001375101.1

ORF Insert Sequence:

ATGACAAAGG AGACTGACAA AAAGCCAAAA GAACGTCCGA ATCAAAGAAA AGTTGCATTT 
CGAAATGAAG CAATAAATGC ATTAGTAAAT ACAGATGAGG ACCAAGTCAC ATTTTCGAAA 
GCAATGGAAG TTGCCAAATT AGATTGTCAA AAGTGTCTTC TACACGATAT GCATAGTTCA 
AAGTCATCGA ATTGTCGCGA TAATCCATTT TGTATACATC GATTAGGACT CGAGAAGTTT 
GAAAAGTTGA TAACTCAGGA GCAGGAAACT AAAGAAGAAG CGAAAAAAGA TCAAAAGCGG 
AGAGATCTGA ATGATCAACC AGCCGGGCTT ATCAATGGAG GAAACTTTTG CTATGTGAAT 
AGTTTTCTTC AAGTCTGGTT CAACGTTCCT GAGTTCCGGC AGTTGATCTA TGATTTCCGA 
CCATCGGAGA ACTTTGTTCC GCCAGAAGCT CCAAGAATGA ATGTTCAAGC AACTATGCTC 
GCGCTTCAAG ACATTTTTTA TACACTACAG ACAACTCCTT TCAACGAGAC CGACAAAACT 
TCGAATCTTG GGAAATTACT TCGATTGAAT TCAGAACAAC AAGATTCTCA GGAATTCGGA 
TTAAAATTCT TCAATGCACT CGAACGATGT CTTCCAGATC ATCCGAATGG AAAAGAAACT 
TTGAAAAGAT TAAAAGATCT TTTCACTGGA GAGACTTGTA CTCGAATTGT ATGCAAATGT 
GGACAACGAT CTGAACGGGA AGAAACAGCA ATCTCATTGA CTTTGAATAT TGAAGGTTAT 
TGCACACTTC TAGATGCTCT TGATGCTTAC TTTGGAGAAG AACATCTGGA CGACTTCAAA 
TGCTCAAAAT GCAATAAAAC TGGAGATGTT TCGAAGCAAT CCGATTATGT TAAGCTACCT 
CCTGTCATTG TGATTCAGCT CAATCGATAC AAATACACAT CCAAAGGGCG TCAAAAGTTA 
AAAACACCAA TGGCATATCC AAGAGAAATT CCAGCAAAAG CATTTCAAAG AACGAATAAT 
TCAATTCCTC CGCCTGCTGA GATGTATGAT CTTTTCGCAG TTACCATCCA CGAAGGCAAT 
AACGCTGAAT GCGGGCATTA CTATGATCTT ATCAAATCAC CGCTCAATCA GAAATGGTAT 
CGGTACAATG ACGAGGCGAT CGAAGCCATC CCAAAGCCAC CTGGAACTGA GAAACCCACC 
ACAGCAAAAA CTGAAAAATC ACGGAAAAAA GATAAAGAAA AATATCCAAC GGATCAAAAA 
GCATGTTATG GACTTCTTTA CCGGAGACGT GATGCATTTA AACCACTTCC ACATCCAAAA 
CTTCCTCCAG AAGAATTGAT TATTGACTCG AAAACTGAGA TCGAAGAATT ATTCGAAGGA 
TTGACGAAAA AGAAAATTGA GAAGAGTGAA AAGAGATTGT ATGATCTTGA GAGACGGATT 
AACAAGGTGA AGATTTCGTA TGGAAAGCTG GAAACACATA GCGATAAATA TAAAGAAGCA 
AATGAAGTTG TCTTTTTGCC AACAACTCTT CTGCAGGATG TATTAGCTCA GGAATATGAA 
GTTGCAAAAG GAGAAAAGAA GAAAAAGAAG AAAGAAGCAT CGGAAAATGA GGAGAAAAAG 
AAAAATGAAG AGGATGAAGC ATTGTCCGCC GCGATCGCTG CATCTGAAGC CGATCAGAGA 
GATAAAGCTT CGTCTGAACC TTCAACTTCT GCTGCTGCAA CAGAAGCAGG AGATGATGAG 
GAGTTGAGAG CAGAAAGTGA AACACCGAAT CCTGAAAATG CTGAATCAAC ACAAGTTGCT 
ATCATGGAAA CAGATGAGAT CATGGATACA ACTCCCACAA AAGATATTGA TATTCTCGCG 
AAGGCAATGG AAGATAATGC ACTCCCAACC GTTGAGGTGC CACAACCAGA ACTCAAAAAA 
CGAACTCGTC AGCAGAATGG AGAAGTCAAA TACGTGTATT CTCAACGAAC TCCTAGAAAA 
AGCCATAATG GGACGAATGG AACTAACTCG AGTCCCCAGA AACAACCGGT TTCATCACGA 
GTTGCGGCTC TGCTGAGTTC TCATGAAATT CCAACATGTG GTCACGGAAA GATGTCAATC 
GATCCAATTC TTTACGGAGA TGTCAAAGCA GTATCACGGG CTCCAGCAAT CGCCTTGCTC 
CGTGAGTATG ACTTTCGAGT GAAAATCGTG TATGATAATG GAGAGAACGT TTTCCCGGAA 
AATGAAAAGG AACGAGATGT TTTTATTTTC ACAGCAGAAG ACATTTGTAT GGAATGTGTG 
AGGGAAATGC GTGAGGAAGG AAATTTTAAC AATCAACTGG AAGACGATGA AAAGCTTGTT 
CGACGTATTC TGAAAGAAGA AAAGCAACGG TGTTCTGTGA AATGCCCTTC CGAACGGCCC 
GATGGATACC TTTACGTAGC TAAATTTGCA CTTTCCAACT TCAAGAAGTC AGCCATGTCA 
GCTCGCGAGA ACCGACTTGC TCAATCACAT AACAAACAAG GAACACTTCA TTTTGACTCT 
CATCCGATGT TTCAGCAAAA ATCGAATTCT GGCTACCTAA CGTTGTCGTT GAAAAGGACT 
CGAGGAAAAC CTCGCAAATC ATTGTCGGAG ATTCCTGAAA AGATGCAGAA GCTTGATGAA 
ATTGGATCGA AAGAATTGCC AGATGAGATT ATTGCTGATG AAGAAGAAAT TTCGGAAAAT 
ATGGGATCCG ATATACCAAC AAAACCGGTG GAGTCGATAA ATCCAGATGC GCTTGTTCCC 
TTCGAGAAGA TTGAATTTAA TTCAGAGCTT CGTTGTTCAC ACGGAGGAAT CAATTTTAAT 
CAATTCAGAC TATCTGTTTC TCCCGAAGAG TGGGCTCATT TAAAAGTCTA TTTCGATGAA 
TGTTACGAGG TGAAATGTTC GGACGACGTG TGTGATCAGT GTCGACAAAT GGAGGTTGAC 
GCACAAAACG GATCCGAGAA CATGCGTGGG TTGGTCAGAG AGATGCGCAA ACGAATCAGT 
GACACATTAA AAACCGTTGA ATCAAGAGCT GAATCTAAAG AAGATGGTGC AGACATAAAG 
TATGGAATCT GTAGTGTCTT TATAGATAAG CTTAGAAAGC TGACGAGTCG ACAATCTACG 
AGTCCGCCAT CTATCTGCCA AGAGTGCCTT CTTTGTCCAC ATCAGCAGCC GTTTAAAGGA 
TTTTTGAATG AAGATAATCA CAAAGATTCA CACGTCGTCG GTCTTACCGA AGAGGAATGG 
AACACGTTTC TTACAGAAAT AAGGAAACTT GAAGAAGCTG GTGATGATCA ATCGATTGCG 
GTAGATCCTT GTCCAATCCC CATTGAAAAT GGTCAAATTG TGGACATGTG CGAAAAGTGT 
TTTGAGCAAC ATATAAAGTT CACTGAAGAA CAGAAATACA TGTTTGAAAA TGAGAATATA 
TATGTGAAAC TTGTGAATTT GAATGTCGAG GAAGATATTG CGAAGGCGAA TGGGAAAGCT 
AGAAGAGGAC GAGCTAAGAA CTTGTATGCT ATAAAGATGT CCTCGACGAA TAAGCTGATG 
GAGCTGAAAG TTCAATTGTA TGATAAAACA CACCAATTAC CAAACGATCA ACTATTGTAT 
CGTACAGCCG GCGGTGAACA GTTTGATGTT AGCAACAATC AGAAAACATT GTTCGATCTT 
CGTCTTTCTC CAAATAATAA CGATAATCCT CTCATCCTGA TTGCTCAACA GTTTTCTCCA 
TCTGCGTCTC AAGCTGATGA AACTGGTGAT CGTGCCCCGG AGCGTGGTTT CGTGGATACA 
GCGCTCGCTC ATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

usp-48 ( NM_001375102.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001362163.1 Caenorhabditis elegans Ubiquitin Specific Protease (usp-48), mRNA.

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CloneID	OCa850864
Clone ID Related Accession (Same CDS sequence)	NM_001375102.1
Accession Version	NM_001375102.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3789bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Ubiquitin Specific Protease
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003279).

ATGACAAAGG AGACTGACAA AAAGCCAAAA GAACGTCCGA ATCAAAGAAA AGTTGCATTT 
CGAAATGAAG CAATAAATGC ATTAGTAAAT ACAGATGAGG ACCAAGTCAC ATTTTCGAAA 
GCAATGGAAG TTGCCAAATT AGATTGTCAA AAGTGTCTTC TACACGATAT GCATAGTTCA 
AAGTCATCGA ATTGTCGCGA TAATCCATTT TGTATACATC GATTAGGACT CGAGAAGTTT 
GAAAAGTTGA TAACTCAGGA GCAGGAAACT AAAGAAGAAG CGAAAAAAGA TCAAAAGCGG 
AGAGATCTGA ATGATCAACC AGCCGGGCTT ATCAATGGAG GAAACTTTTG CTATGTGAAT 
AGTTTTCTTC AAGTCTGGTT CAACGTTCCT GAGTTCCGGC AGTTGATCTA TGATTTCCGA 
CCATCGGAGA ACTTTGTTCC GCCAGAAGCT CCAAGAATGA ATGTTCAAGC AACTATGCTC 
GCGCTTCAAG ACATTTTTTA TACACTACAG ACAACTCCTT TCAACGAGAC CGACAAAACT 
TCGAATCTTG GGAAATTACT TCGATTGAAT TCAGAACAAC AAGATTCTCA GGAATTCGGA 
TTAAAATTCT TCAATGCACT CGAACGATGT CTTCCAGATC ATCCGAATGG AAAAGAAACT 
TTGAAAAGAT TAAAAGATCT TTTCACTGGA GAGACTTGTA CTCGAATTGT ATGCAAATGT 
GGACAACGAT CTGAACGGGA AGAAACAGCA ATCTCATTGA CTTTGAATAT TGAAGGTTAT 
TGCACACTTC TAGATGCTCT TGATGCTTAC TTTGGAGAAG AACATCTGGA CGACTTCAAA 
TGCTCAAAAT GCAATAAAAC TGGAGATGTT TCGAAGCAAT CCGATTATGT TAAGCTACCT 
CCTGTCATTG TGATTCAGCT CAATCGATAC AAATACACAT CCAAAGGGCG TCAAAAGTTA 
AAAACACCAA TGGCATATCC AAGAGAAATT CCAGCAAAAG CATTTCAAAG AACGAATAAT 
TCAATTCCTC CGCCTGCTGA GATGTATGAT CTTTTCGCAG TTACCATCCA CGAAGGCAAT 
AACGCTGAAT GCGGGCATTA CTATGATCTT ATCAAATCAC CGCTCAATCA GAAATGGTAT 
CGGTACAATG ACGAGGCGAT CGAAGCCATC CCAAAGCCAC CTGGAACTGA GAAACCCACC 
ACAGCAAAAA CTGAAAAATC ACGGAAAAAA GATAAAGAAA AATATCCAAC GGATCAAAAA 
GCATGTTATG GACTTCTTTA CCGGAGACGT GATGCATTTA AACCACTTCC ACATCCAAAA 
CTTCCTCCAG AAGAATTGAT TATTGACTCG AAAACTGAGA TCGAAGAATT ATTCGAAGGA 
TTGACGAAAA AGAAAATTGA GAAGAGTGAA AAGAGATTGT ATGATCTTGA GAGACGGATT 
AACAAGGTGA AGATTTCGTA TGGAAAGCTG GAAACACATA GCGATAAATA TAAAGAAGCA 
AATGAAGTTG TCTTTTTGCC AACAACTCTT CTGCAGGATG TATTAGCTCA GGAATATGAA 
GTTGCAAAAG GAGAAAAGAA GAAAAAGAAG AAAGAAGCAT CGGAAAATGA GGAGAAAAAG 
AAAAATGAAG AGGATGAAGC ATTGTCCGCC GCGATCGCTG CATCTGAAGC CGATCAGAGA 
GATAAAGCTT CGTCTGAACC TTCAACTTCT GCTGCTGCAA CAGAAGCAGG AGATGATGAG 
GAGTTGAGAG CAGAAAGTGA AACACCGAAT CCTGAAAATG CTGAATCAAC ACAAGTTGCT 
ATCATGGAAA CAGATGAGAT CATGGATACA ACTCCCACAA AAGATATTGA TATTCTCGCG 
AAGGCAATGG AAGATAATGC ACTCCCAACC GTTGAGGTGC CACAACCAGA ACTCAAAAAA 
CGAACTCGTC AGCAGAATGG AGAAGTCAAA TACGTGTATT CTCAACGAAC TCCTAGAAAA 
AGCCATAATG GGACGAATGG AACTAACTCG AGTCCCCAGA AACAACCGGT TTCATCACGA 
GTTGCGGCTC TGCTGAGTTC TCATGAAATT CCAACATGTG GTCACGGAAA GATGTCAATC 
GATCCAATTC TTTACGGAGA TGTCAAAGCA GTATCACGGG CTCCAGCAAT CGCCTTGCTC 
CGTGAGTATG ACTTTCGAGT GAAAATCGTG TATGATAATG GAGAGAACGT TTTCCCGGAA 
AATGAAAAGG AACGAGATGT TTTTATTTTC ACAGCAGAAG ACATTTGTAT GGAATGTGTG 
AGGGAAATGC GTGAGGAAGG AAATTTTAAC AATCAACTGG AAGACGATGA AAAGCTTGTT 
CGACGTATTC TGAAAGAAGA AAAGCAACGG TGTTCTGTGA AATGCCCTTC CGAACGGCCC 
GATGGATACC TTTACGTAGC TAAATTTGCA CTTTCCAACT TCAAGAAGTC AGCCATGTCA 
GCTCGCGAGA ACCGACTTGC TCAATCACAT AACAAACAAG GAACACTTCA TTTTGACTCT 
CATCCGATGC AAAAATCGAA TTCTGGCTAC CTAACGTTGT CGTTGAAAAG GACTCGAGGA 
AAACCTCGCA AATCATTGTC GGAGATTCCT GAAAAGATGC AGAAGCTTGA TGAAATTGGA 
TCGAAAGAAT TGCCAGATGA GATTATTGCT GATGAAGAAG AAATTTCGGA AAATATGGGA 
TCCGATATAC CAACAAAACC GGTGGAGTCG ATAAATCCAG ATGCGCTTGT TCCCTTCGAG 
AAGATTGAAT TTAATTCAGA GCTTCGTTGT TCACACGGAG GAATCAATTT TAATCAATTC 
AGACTATCTG TTTCTCCCGA AGAGTGGGCT CATTTAAAAG TCTATTTCGA TGAATGTTAC 
GAGGTGAAAT GTTCGGACGA CGTGTGTGAT CAGTGTCGAC AAATGGAGGT TGACGCACAA 
AACGGATCCG AGAACATGCG TGGGTTGGTC AGAGAGATGC GCAAACGAAT CAGTGACACA 
TTAAAAACCG TTGAATCAAG AGCTGAATCT AAAGAAGATG GTGCAGACAT AAAGTATGGA 
ATCTGTAGTG TCTTTATAGA TAAGCTTAGA AAGCTGACGA GTCGACAATC TACGAGTCCG 
CCATCTATCT GCCAAGAGTG CCTTCTTTGT CCACATCAGC AGCCGTTTAA AGGATTTTTG 
AATGAAGATA ATCACAAAGA TTCACACGTC GTCGGTCTTA CCGAAGAGGA ATGGAACACG 
TTTCTTACAG AAATAAGGAA ACTTGAAGAA GCTGGTGATG ATCAATCGAT TGCGGTAGAT 
CCTTGTCCAA TCCCCATTGA AAATGGTCAA ATTGTGGACA TGTGCGAAAA GTGTTTTGAG 
CAACATATAA AGTTCACTGA AGAACAGAAA TACATGTTTG AAAATGAGAA TATATATGTG 
AAACTTGTGA ATTTGAATGT CGAGGAAGAT ATTGCGAAGG CGAATGGGAA AGCTAGAAGA 
GGACGAGCTA AGAACTTGTA TGCTATAAAG ATGTCCTCGA CGAATAAGCT GATGGAGCTG 
AAAGTTCAAT TGTATGATAA AACACACCAA TTACCAAACG ATCAACTATT GTATCGTACA 
GCCGGCGGTG AACAGTTTGA TGTTAGCAAC AATCAGAAAA CATTGTTCGA TCTTCGTCTT 
TCTCCAAATA ATAACGATAA TCCTCTCATC CTGATTGCTC AACAGTTTTC TCCATCTGCG 
TCTCAAGCTG ATGAAACTGG TGATCGTGCC CCGGAGCGTG GTTTCGTGGA TACAGCGCTC 
GCTCATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001362163.1
CDS	4..3792
Translation	MTKETDKKPKERPNQRKVAFRNEAINALVNTDEDQVTFSKAMEVAKLDCQKCLLHDMHSSKSSNCRDNPFCIHRLGLEKFEKLITQEQETKEEAKKDQKRRDLNDQPAGLINGGNFCYVNSFLQVWFNVPEFRQLIYDFRPSENFVPPEAPRMNVQATMLALQDIFYTLQTTPFNETDKTSNLGKLLRLNSEQQDSQEFGLKFFNALERCLPDHPNGKETLKRLKDLFTGETCTRIVCKCGQRSEREETAISLTLNIEGYCTLLDALDAYFGEEHLDDFKCSKCNKTGDVSKQSDYVKLPPVIVIQLNRYKYTSKGRQKLKTPMAYPREIPAKAFQRTNNSIPPPAEMYDLFAVTIHEGNNAECGHYYDLIKSPLNQKWYRYNDEAIEAIPKPPGTEKPTTAKTEKSRKKDKEKYPTDQKACYGLLYRRRDAFKPLPHPKLPPEELIIDSKTEIEELFEGLTKKKIEKSEKRLYDLERRINKVKISYGKLETHSDKYKEANEVVFLPTTLLQDVLAQEYEVAKGEKKKKKKEASENEEKKKNEEDEALSAAIAASEADQRDKASSEPSTSAAATEAGDDEELRAESETPNPENAESTQVAIMETDEIMDTTPTKDIDILAKAMEDNALPTVEVPQPELKKRTRQQNGEVKYVYSQRTPRKSHNGTNGTNSSPQKQPVSSRVAALLSSHEIPTCGHGKMSIDPILYGDVKAVSRAPAIALLREYDFRVKIVYDNGENVFPENEKERDVFIFTAEDICMECVREMREEGNFNNQLEDDEKLVRRILKEEKQRCSVKCPSERPDGYLYVAKFALSNFKKSAMSARENRLAQSHNKQGTLHFDSHPMQKSNSGYLTLSLKRTRGKPRKSLSEIPEKMQKLDEIGSKELPDEIIADEEEISENMGSDIPTKPVESINPDALVPFEKIEFNSELRCSHGGINFNQFRLSVSPEEWAHLKVYFDECYEVKCSDDVCDQCRQMEVDAQNGSENMRGLVREMRKRISDTLKTVESRAESKEDGADIKYGICSVFIDKLRKLTSRQSTSPPSICQECLLCPHQQPFKGFLNEDNHKDSHVVGLTEEEWNTFLTEIRKLEEAGDDQSIAVDPCPIPIENGQIVDMCEKCFEQHIKFTEEQKYMFENENIYVKLVNLNVEEDIAKANGKARRGRAKNLYAIKMSSTNKLMELKVQLYDKTHQLPNDQLLYRTAGGEQFDVSNNQKTLFDLRLSPNNNDNPLILIAQQFSPSASQADETGDRAPERGFVDTALAH

Target ORF information:

RefSeq Version	NM_001375102.1
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Ubiquitin Specific Protease (usp-48), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001375102.1

ORF Insert Sequence:

ATGACAAAGG AGACTGACAA AAAGCCAAAA GAACGTCCGA ATCAAAGAAA AGTTGCATTT 
CGAAATGAAG CAATAAATGC ATTAGTAAAT ACAGATGAGG ACCAAGTCAC ATTTTCGAAA 
GCAATGGAAG TTGCCAAATT AGATTGTCAA AAGTGTCTTC TACACGATAT GCATAGTTCA 
AAGTCATCGA ATTGTCGCGA TAATCCATTT TGTATACATC GATTAGGACT CGAGAAGTTT 
GAAAAGTTGA TAACTCAGGA GCAGGAAACT AAAGAAGAAG CGAAAAAAGA TCAAAAGCGG 
AGAGATCTGA ATGATCAACC AGCCGGGCTT ATCAATGGAG GAAACTTTTG CTATGTGAAT 
AGTTTTCTTC AAGTCTGGTT CAACGTTCCT GAGTTCCGGC AGTTGATCTA TGATTTCCGA 
CCATCGGAGA ACTTTGTTCC GCCAGAAGCT CCAAGAATGA ATGTTCAAGC AACTATGCTC 
GCGCTTCAAG ACATTTTTTA TACACTACAG ACAACTCCTT TCAACGAGAC CGACAAAACT 
TCGAATCTTG GGAAATTACT TCGATTGAAT TCAGAACAAC AAGATTCTCA GGAATTCGGA 
TTAAAATTCT TCAATGCACT CGAACGATGT CTTCCAGATC ATCCGAATGG AAAAGAAACT 
TTGAAAAGAT TAAAAGATCT TTTCACTGGA GAGACTTGTA CTCGAATTGT ATGCAAATGT 
GGACAACGAT CTGAACGGGA AGAAACAGCA ATCTCATTGA CTTTGAATAT TGAAGGTTAT 
TGCACACTTC TAGATGCTCT TGATGCTTAC TTTGGAGAAG AACATCTGGA CGACTTCAAA 
TGCTCAAAAT GCAATAAAAC TGGAGATGTT TCGAAGCAAT CCGATTATGT TAAGCTACCT 
CCTGTCATTG TGATTCAGCT CAATCGATAC AAATACACAT CCAAAGGGCG TCAAAAGTTA 
AAAACACCAA TGGCATATCC AAGAGAAATT CCAGCAAAAG CATTTCAAAG AACGAATAAT 
TCAATTCCTC CGCCTGCTGA GATGTATGAT CTTTTCGCAG TTACCATCCA CGAAGGCAAT 
AACGCTGAAT GCGGGCATTA CTATGATCTT ATCAAATCAC CGCTCAATCA GAAATGGTAT 
CGGTACAATG ACGAGGCGAT CGAAGCCATC CCAAAGCCAC CTGGAACTGA GAAACCCACC 
ACAGCAAAAA CTGAAAAATC ACGGAAAAAA GATAAAGAAA AATATCCAAC GGATCAAAAA 
GCATGTTATG GACTTCTTTA CCGGAGACGT GATGCATTTA AACCACTTCC ACATCCAAAA 
CTTCCTCCAG AAGAATTGAT TATTGACTCG AAAACTGAGA TCGAAGAATT ATTCGAAGGA 
TTGACGAAAA AGAAAATTGA GAAGAGTGAA AAGAGATTGT ATGATCTTGA GAGACGGATT 
AACAAGGTGA AGATTTCGTA TGGAAAGCTG GAAACACATA GCGATAAATA TAAAGAAGCA 
AATGAAGTTG TCTTTTTGCC AACAACTCTT CTGCAGGATG TATTAGCTCA GGAATATGAA 
GTTGCAAAAG GAGAAAAGAA GAAAAAGAAG AAAGAAGCAT CGGAAAATGA GGAGAAAAAG 
AAAAATGAAG AGGATGAAGC ATTGTCCGCC GCGATCGCTG CATCTGAAGC CGATCAGAGA 
GATAAAGCTT CGTCTGAACC TTCAACTTCT GCTGCTGCAA CAGAAGCAGG AGATGATGAG 
GAGTTGAGAG CAGAAAGTGA AACACCGAAT CCTGAAAATG CTGAATCAAC ACAAGTTGCT 
ATCATGGAAA CAGATGAGAT CATGGATACA ACTCCCACAA AAGATATTGA TATTCTCGCG 
AAGGCAATGG AAGATAATGC ACTCCCAACC GTTGAGGTGC CACAACCAGA ACTCAAAAAA 
CGAACTCGTC AGCAGAATGG AGAAGTCAAA TACGTGTATT CTCAACGAAC TCCTAGAAAA 
AGCCATAATG GGACGAATGG AACTAACTCG AGTCCCCAGA AACAACCGGT TTCATCACGA 
GTTGCGGCTC TGCTGAGTTC TCATGAAATT CCAACATGTG GTCACGGAAA GATGTCAATC 
GATCCAATTC TTTACGGAGA TGTCAAAGCA GTATCACGGG CTCCAGCAAT CGCCTTGCTC 
CGTGAGTATG ACTTTCGAGT GAAAATCGTG TATGATAATG GAGAGAACGT TTTCCCGGAA 
AATGAAAAGG AACGAGATGT TTTTATTTTC ACAGCAGAAG ACATTTGTAT GGAATGTGTG 
AGGGAAATGC GTGAGGAAGG AAATTTTAAC AATCAACTGG AAGACGATGA AAAGCTTGTT 
CGACGTATTC TGAAAGAAGA AAAGCAACGG TGTTCTGTGA AATGCCCTTC CGAACGGCCC 
GATGGATACC TTTACGTAGC TAAATTTGCA CTTTCCAACT TCAAGAAGTC AGCCATGTCA 
GCTCGCGAGA ACCGACTTGC TCAATCACAT AACAAACAAG GAACACTTCA TTTTGACTCT 
CATCCGATGC AAAAATCGAA TTCTGGCTAC CTAACGTTGT CGTTGAAAAG GACTCGAGGA 
AAACCTCGCA AATCATTGTC GGAGATTCCT GAAAAGATGC AGAAGCTTGA TGAAATTGGA 
TCGAAAGAAT TGCCAGATGA GATTATTGCT GATGAAGAAG AAATTTCGGA AAATATGGGA 
TCCGATATAC CAACAAAACC GGTGGAGTCG ATAAATCCAG ATGCGCTTGT TCCCTTCGAG 
AAGATTGAAT TTAATTCAGA GCTTCGTTGT TCACACGGAG GAATCAATTT TAATCAATTC 
AGACTATCTG TTTCTCCCGA AGAGTGGGCT CATTTAAAAG TCTATTTCGA TGAATGTTAC 
GAGGTGAAAT GTTCGGACGA CGTGTGTGAT CAGTGTCGAC AAATGGAGGT TGACGCACAA 
AACGGATCCG AGAACATGCG TGGGTTGGTC AGAGAGATGC GCAAACGAAT CAGTGACACA 
TTAAAAACCG TTGAATCAAG AGCTGAATCT AAAGAAGATG GTGCAGACAT AAAGTATGGA 
ATCTGTAGTG TCTTTATAGA TAAGCTTAGA AAGCTGACGA GTCGACAATC TACGAGTCCG 
CCATCTATCT GCCAAGAGTG CCTTCTTTGT CCACATCAGC AGCCGTTTAA AGGATTTTTG 
AATGAAGATA ATCACAAAGA TTCACACGTC GTCGGTCTTA CCGAAGAGGA ATGGAACACG 
TTTCTTACAG AAATAAGGAA ACTTGAAGAA GCTGGTGATG ATCAATCGAT TGCGGTAGAT 
CCTTGTCCAA TCCCCATTGA AAATGGTCAA ATTGTGGACA TGTGCGAAAA GTGTTTTGAG 
CAACATATAA AGTTCACTGA AGAACAGAAA TACATGTTTG AAAATGAGAA TATATATGTG 
AAACTTGTGA ATTTGAATGT CGAGGAAGAT ATTGCGAAGG CGAATGGGAA AGCTAGAAGA 
GGACGAGCTA AGAACTTGTA TGCTATAAAG ATGTCCTCGA CGAATAAGCT GATGGAGCTG 
AAAGTTCAAT TGTATGATAA AACACACCAA TTACCAAACG ATCAACTATT GTATCGTACA 
GCCGGCGGTG AACAGTTTGA TGTTAGCAAC AATCAGAAAA CATTGTTCGA TCTTCGTCTT 
TCTCCAAATA ATAACGATAA TCCTCTCATC CTGATTGCTC AACAGTTTTC TCCATCTGCG 
TCTCAAGCTG ATGAAACTGG TGATCGTGCC CCGGAGCGTG GTTTCGTGGA TACAGCGCTC 
GCTCATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.
Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

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usp-48 cDNA ORF clone, Caenorhabditis elegans

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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