nmy-2 cDNA ORF clone, Caenorhabditis elegans

Gene
Clones
gRNAs

Summary

Gene Symbol	nmy-2
Entrez Gene ID	172562
Full Name	Non-muscle MYosin
Gene Type	protein-coding
Organism	Caenorhabditis elegans

mRNA and Protein(s)

mRNA	Protein	Name
NM_059785.7	NP_492186.3	Non-muscle MYosin
NM_059785.6	NP_492186.3	Non-muscle MYosin

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	myh11b	XP_694439.6
Caenorhabditis elegans (roundworm)	nmy-2	NP_492186.3

Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.

Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

asymmetric division found in neuroblast lineage; more myosin II accumulated anteriorly, suggesting asymmetric contractile forces may produce different-sized daughters; balance of myosin on the 2 sides of dividing cell can govern size and fate of daughters
Title: Polarized myosin produces unequal-size daughters during asymmetric cell division.

NMY-2 maintains cellular asymmetry and cell boundaries, and promotes a SRC-dependent asymmetric cell division
Title: NMY-2 maintains cellular asymmetry and cell boundaries, and promotes a SRC-dependent asymmetric cell division.

UNC-45 and NMY-2 are required for fertility since the lack of either component results in complete sterility
Title: UNC-45 is required for NMY-2 contractile function in early embryonic polarity establishment and germline cellularization in C. elegans.

These results suggest that Galpha signaling and LET-99 control centration by regulating polarized actomyosin contraction.
Title: Control of nuclear centration in the C. elegans zygote by receptor-independent Galpha signaling and myosin II.

Study concludes that the two pathways of cleavage-furrow formation are mechanistically distinct; in particular, the astral pathway for cleavage-furrow formation involves the negative regulation of myosin distribution by astral cues.
Title: Astral signals spatially bias cortical myosin recruitment to break symmetry and promote cytokinesis.

The following nmy-2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the nmy-2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa335303	NM_059785.7 Latest version!	Caenorhabditis elegans Non-muscle MYosin (nmy-2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
View Old Accession Versions >>
OCa335303	NM_059785.6	Caenorhabditis elegans Non-muscle MYosin (nmy-2), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

nmy-2 ( NM_059785.7 ) cDNA ORF clone, Caenorhabditis elegans -> NP_492186.3 Caenorhabditis elegans Non-muscle MYosin (nmy-2), mRNA.

Price & Availability↑

CloneID	OCa335303
Clone ID Related Accession (Same CDS sequence)	NM_059785.7 , NM_059785.6
Accession Version	NM_059785.7 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6012bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Non-muscle MYosin
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003279). On Sep 4, 2019 this sequence version replaced NM_059785.6.

ATGACATCAT CTCGACAAAA AGATGATGAG ATTGACCAAT TGAGAGCCAA GACAGACTTC 
CTCAATCCAT CGATTTCGGC AGATTGGGCG AAAAAAAAGT TGATGTGGGT ACCCAGTGAG 
AAGGATGGAT TCGCACTTGG AGCTGTCATC GGGGAACCAC ATGCAGACGG AACTATCGAC 
ATTGAGCTTA TGGAGACTGG CGAACGGCAG CGTGTTTCGT CTGATGATTG CCAGAAGCCA 
AATCCTCCGA AATATGACAA ATGCGAGGAT ATGTCAATGC TGACGTGTCT GAATGAAGCG 
TCGGTTCTTC ATAATCTAAA GCAACGATAT TTCTCGAATC TCTACTACAC ATACTCTGGC 
TTGTTCTGCG TTGTAATAAA TCCATACAAA CGAATTCCAA TCTACACGGA TACAATTGCT 
GAACAGTTCA AATGCAAAAA GAGAAAGGAG ATGCCACCAC ACATATTTGC TGTTGCCGAT 
GAAGCCTACC GCAGCATGCT TCAAGAACGA GATGATCAGT CAATTCTCTG CACTGGAGAA 
TCGGGAGCTG GAAAGACTGA AAATACAAAG AAAGTGATTC AGTATTTGGC TTATGTGGCA 
AATCGGTCGA TGGTGAAGAA TCGGAAGACT TCCGTTGATC TGGACACATC TACAAACCGG 
ATCATGGGCC AGCTTGAGGA ACAGCTTTTG CAAGCAAATC CCATACTCGA GGCTTTCGGA 
AACAGTAAGA CAGTGAAGAA CGATAATTCC AGTAGATTTG GAAAGTTCAT TCGCGTTCAT 
TTCGACAGCA CAGGGTGCAT TTCTGGAGCG AATATTGAAT TTTATCTTCT GGAGAAATCT 
CGCGTGCTGA AACAAGCTCC CAATGAGCGA TCGTTCCACA TTTTCTACCA GTTGCTGAAA 
GGACTCTCAA AAGTACAGAG AGAACATTAT CTCCTAGAAG ACAGCCTATC CAAGTACAAG 
TTTGTCTCAA ATGGTGACAG TAAATTGGCT GGAGTTGATG ATGGAGCAGA GATGAAGGAA 
ACACTGAATG CCATGTCAAT TATGGGATTG AACGACGAAG AGATTGGAGG AATACTTAGA 
GTTGTCTCGG CAGTTATGCT CTTTGGAAAT CTTGAATTTT CTCACGAAAA CAAGAATAAC 
GATCAGGCTG TTCTGCTCAA TGACGCTGTT GCTCAAAAAA TCGCTTCACT TCTCGGAGTC 
AATGTTACCG AATTGATGCG TGCATTCTTG AAGCCAAAAA TCAAGGTTCA AAGGGATTTG 
GTCCATCGAG CTCAAAGCGT AGACCAAGTC AATTTCTCTG TGGGAGCCAT TGCGAAGGCC 
TCGTATGAGC GACTCTTCAG ATGGTTGGTT CATCGCCTCA ACAAATCTCT CGATCGGACC 
CGTCAACAAT CTGTCAGCTT TATCGGAATT CTTGATATTG CTGGTTTTGA AATTTTTGAA 
ACAAACTCAT TCGAGCAGCT ATGTATCAAC TACACTAACG AGAAACTTCA ACAACTCTTC 
AACAATACAA TGTTCGTTCG TGAACAGCAA GAATATCTTG ATGAAGGATT GGAGTGGAAG 
TTCTTGGATT TCGGCCTCAA TCTTCAACCG ACTATTGACT TGATTGATAA GCCTATGGGA 
ATCATGTCAA CTCTTGATGA TGTTTGTCTA TTCCCACAAG GAAATGATCA ATCATTTGTT 
CAACGCCTCA ATAACACTCA TTCTCAACAT CCGAAGTACG TCGTTCCAGA GATTCGCTCA 
CGAAGCGATT TTGCCGTTGT TCATTATGCG GGCCGAGTCG ACTATCAATC TGAAGGTTGG 
CGCGTGAAAA ACATGGATCC ATTGAATGAG AATGTGATTG ATGTGCTCAA AACTTCAAAG 
GAATCTCTTA TTGTCGACAT GTGGAAAGAT ATCGCTGATG TTTGCAGTTT GTCAGCTGCT 
GACAGTACTT CAGATACCGG AGTATTTGGA TCTCGTGTTC CAAAGAAGGG AATGTTCCGT 
ACTGTTTCTC AACTTTACAA AGAACAATTG GCTCGTCTTA TGAGTACTCT CAATAACACC 
AATCCTCACT TTGTTCGCTG CATCATTCCA AATCACGAAA AGAAACATGG AGTTCTCAAC 
GCTCATCTTG TTCTTGATCA ATTAAGATGC AACGGAGTGT TGGAAGGAAT TCGTATATGC 
CGTCAAGGAT TCCCTACGCG GCTCCCGTTC CAAGAATTCC GTCAACGCTA CGAGAAACTT 
CTTGCCCCTG ATGTCAACCC AGCTGGATTC ATGGACGGTA AAAACGCCGT TTATCGTATT 
GTTCAATATC TGGAAGTTGA CGCCAACCTC TTCCGTATTG GTCAATCTAA GATCTTCTTC 
CGTTCTGGAG TTATAGCTGA GTTTGAGGAG ATGCGTGATC AGAAGCTTTC CGCTCTTATT 
GAATCTTTCC AAGCTCAATG TCGTGGATGG CTAGGACGCC GAGTGATGGT ACGCAGACGA 
GAACAAGAAG TTGCTATCAA AATTCTTCAA AGAAATGGTT TGGCATGGAT GAGACTGCGT 
GAATGGCAAT GGTGGAGACT TCTGACCAAA GTGAAGCCAC TTCTCGAAGT TACCAACAAA 
GACGAATTAA TTGCTGAGCG AGAACAAGAA CTGAAAGTGA CAGCTGAAAA GCTTCGTAGA 
TCCGAGGTAT TCATCTCCGA TTACAAGCAA CAAATGGAAA AAATGGACGA GGAGCGGCTG 
GTTTTAAAGA CGAGACTCGA TGCTGAATCG TCTGAGAGAG CTGAAATATT TGAGGAACGT 
TCCCGAATGG CTGCTCGGAG AGACGAACTT GAAGGAATCC TTGAAGAAGT TTCGAAGAGA 
CTGGAGATCG AAGAGCAAAA GGCAAAGAAG GCTGATTCTG AATCCCGAAA ATTGACGGAG 
ATGGTTAGAC ATCTCGAAGA GAATCTTGAA GATGAAGAAA GAAGCCGTCA GAAATTGTTG 
CTCGAAAAAA ATTCAATTGA ATCTCGGTTG AAGGAACTGG AAGCACAAGG ACTCGAGCTT 
GAAGATTCTG GAAACAAGTT GTCGAAAGAA AAGAAGGCTT TGGAAGAACG CTGTGAAGAT 
CTTTCGTCAC GTTTAATTGA CGAGGTTGAA CGATCGAAAC AATTGGTCAA GGCAAAGGCT 
CGCCTGGAAG CTACGGTTGC AGAGATAAAC GATGAATTGG AAAAAGAGAA GCAGCAGAGA 
CATAATGCCG AAACAGCTCG TCGTGCTGCC GAAACACAGC TCCGCGAGGA ACAAGAATCA 
TGCCTGGAAA AGACTCGTAA AGCGGAAGAG TTGACAAATC AGCTGATGCG CAAAGAGTCG 
GAATTGTCGC AAATAAGCAT TCGGAACGAC GAAGAACTCG CAGCTCGGCA ACAGTTAGAA 
CGTGAAATTC GGGAAATTCG TGCACAGCTT GACGATGCAA TTGAGGAGAC AAACAAGGAG 
AAAGCTGCTC GTCAGAAAGC AGAGAAAGCT CGTCGAGATA TGGCTGAAGA ATTGGAATCC 
TACAAGCAAG AATTGGAAGA ATCCAATGAT AAAACCGTTC TCCACTCGCA ACTCAAAGCT 
AAACGAGATG AAGAGTATGC TCATTTGCAG AAACAACTTG AGGAAACAGT CAAATCTTCT 
GAAGAAGTTG TGGAGGAAAT GAAGGCTCAG AATCAGAAAA AGATTGAGGA GTTGAATGAG 
ACGATCGATC AGTTGAAACG TCAGAAAATT TCGGCTGACA AGGCAAAATC TTCTGCTGAA 
TCTGATAACG AGAACTTCCG TGCCGAGCTG TCCAACATAG CATCGGCTCG TCTGGAGGCG 
GAAAAGAAGC GGAAAGCGGC CGAGACGTCG TTGATGGAGA AGGATCATAA GATGCGTGAA 
ATGCAGTCAA ATCTTGATGA TCTTATGGCT AAGTTATCGA AGATGAACAA CGAGCTTGAA 
AGCATTCAAA AAGCAAAGAG TGCTGACGAA ACTCTGAATT CTAACCTTCT CAAGAAGAAT 
GCTTCACTCG ACATGCAGTT GTCTGAGCTT ACTGAAGCTT CTGAGGAAGA TCGTCGAACT 
AGAGCCACGT TAAACAATAA AATTCGTCAG CTCGAAGAAG ATCTTGCGGT TGCTGTTGAA 
GCCAGAGATG ACGCACTTGA CGCACAAGAA AAGATCGAAA AAGAGGTCAA AGAAGTCAAA 
TCTCTGTTGG CCGAGGCTAG AAAGAAGTTG GATGAGGAAA ATAGAGAGGT GATGGAAGAA 
TTGCGGAAGA AGAAGGAGAA GGAGTTGTCA GCCGAGAAAG AGCGGGCCGA CATGGCTGAA 
CAAGCAAGAG ATAAGGCTGA ACGGGCCAAG AAGAAGGCTA TCCAGGAGGC TGAGGACGTG 
CAAAAAGAGT TGACTGATGT GGTGGCCGCA ACTAGAGAGA TGGAGAGAAA AATGAGGAAG 
TTTGATCAAC AGCTCGCCGA GGAGAGAAAC AACACTTTGC TCGCTCAGCA AGAAAGAGAT 
ATGGCTCATC AAATGCTACG TGATGCAGAG ACGAAGGCTC TGGTGTTGTC AAATGAATTA 
TCCGAGAAAA AGGATATTGT GGATCAGTTA GAAAAGGACA AACGGACTCT CAAACTAGAG 
ATTGACAATT TGGCCTCCAC CAAGGATGAT GCTGGAAAGA ATGTTTACGA GTTGGAGAAG 
ACGAAACGTC GCCTTGATGA AGAGTTGAGT CGTGCGGAAC AACAAATCAT TGAACTAGAA 
GATGCTCTCC AACTTGCTGA TGATGCTAGA AGCAGAGTTG AAGTCAACAT GCAAGCAATG 
AGATCCGAGT TCGAACGTCA ATTAGCCTCT CGCGAAGAAG ATGAGGATGA TCGTAAGAAG 
GGACTCACTT CTAAAATCCG AAATCTTACC GAAGAGCTTG AATCTGAACA ACGTGCTCGT 
CAAGCTGCAA TTGCCAACAA GAAGAAGATC GAATCTCAAA TCAGCGAGTT GACTGAAAAG 
AACGAAGCTT CTTTGAGACA AATCGAAGAT CTTTCGCGTC AGCTTCGCAA AGCCCAACTG 
GGATGGAAAG ATCTCCAGCT GGATGTTACA GAAGCTCGTG CTGCCATGGA AGATGCTCTT 
GCTGGACAAA GAGACGCAGA GAAGAGAGCT CGGGCTTCGG AGGATGAGAT CAAGAGACTC 
ACCGCCGATA TTCAAGCTGT CAGCTCTTCA AAACGTAAAG CAGAAGCTGA GCGCGATGAA 
CTCATTGAAG AAGTTTCTAG TCTTCGCGCT TCCTCATTCA GTAATGAGGA GAAGAGACGT 
CTTGAAGCAA AGGTAATTGA TTTGGAAGAC CAACTCGACG AGGAGGCTTC GGCAAATGAA 
TTGGCACAAG AGAAGGTTCG CAAGTCACAA CAGCAACTAG AACAAATGAC AGCTGATCTT 
GCAATGGAAA GAAGTGTTTG TGAGAGAACA GAATCAGATA AAATTGCATT GGAACGTGCG 
AATCGTGATT TGAAGCAACA ACTTCAAGAT GCTGAAAATA CCGCTGTGGC TCGTCTTCGT 
ACACAAATCA ACGTTGCGGA AGCCAAAGTT TCCAGTCTTG AGCAACAGCT TTCATTGGAA 
GAGCAGGATA AGATGCGTCA AGGCAGAACC CTGCGACGAA TGGAAACGAA AATGGCAGAG 
ATGCAGCAAA TGCTCGAGGA AGAGAAGAGA CAAGGTGAAA GTAATCGTCA GGCTGTTGAT 
CGTCAGAATG CTAGAATCCG TCAACTTCGT ACACAGTTGG AAGATACTGA GGCAGAGCGA 
GACCGTTTGA CAAACAAGTT GAAGGACGAG CGGAGAAGAG CTGAAGAGAT GACTGATCTC 
AATGAGACAC TCTCCAGAGA TGTTTCTTTA TTGAAACAGC GTGAAACGAC AGCTCGTAGA 
ACACCTGGAT TGATTGGACA TCGTGAATCG AGAAGATTTG GAAGCAACAC CAGCCTTGCA 
CGAGATGAAT TCCGTGGATC AGCTCTCACA AACGAAATGT CACCATCTGA CCGTCCAGCT 
TCTCGTCTCA CATCTGGAAC CGGATCCCAA CTTGGCAACA CCGATGAAGA CCGCGACTCA 
GTTCGCAACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_492186.3
CDS	35..6046
Translation	MTSSRQKDDEIDQLRAKTDFLNPSISADWAKKKLMWVPSEKDGFALGAVIGEPHADGTIDIELMETGERQRVSSDDCQKPNPPKYDKCEDMSMLTCLNEASVLHNLKQRYFSNLYYTYSGLFCVVINPYKRIPIYTDTIAEQFKCKKRKEMPPHIFAVADEAYRSMLQERDDQSILCTGESGAGKTENTKKVIQYLAYVANRSMVKNRKTSVDLDTSTNRIMGQLEEQLLQANPILEAFGNSKTVKNDNSSRFGKFIRVHFDSTGCISGANIEFYLLEKSRVLKQAPNERSFHIFYQLLKGLSKVQREHYLLEDSLSKYKFVSNGDSKLAGVDDGAEMKETLNAMSIMGLNDEEIGGILRVVSAVMLFGNLEFSHENKNNDQAVLLNDAVAQKIASLLGVNVTELMRAFLKPKIKVQRDLVHRAQSVDQVNFSVGAIAKASYERLFRWLVHRLNKSLDRTRQQSVSFIGILDIAGFEIFETNSFEQLCINYTNEKLQQLFNNTMFVREQQEYLDEGLEWKFLDFGLNLQPTIDLIDKPMGIMSTLDDVCLFPQGNDQSFVQRLNNTHSQHPKYVVPEIRSRSDFAVVHYAGRVDYQSEGWRVKNMDPLNENVIDVLKTSKESLIVDMWKDIADVCSLSAADSTSDTGVFGSRVPKKGMFRTVSQLYKEQLARLMSTLNNTNPHFVRCIIPNHEKKHGVLNAHLVLDQLRCNGVLEGIRICRQGFPTRLPFQEFRQRYEKLLAPDVNPAGFMDGKNAVYRIVQYLEVDANLFRIGQSKIFFRSGVIAEFEEMRDQKLSALIESFQAQCRGWLGRRVMVRRREQEVAIKILQRNGLAWMRLREWQWWRLLTKVKPLLEVTNKDELIAEREQELKVTAEKLRRSEVFISDYKQQMEKMDEERLVLKTRLDAESSERAEIFEERSRMAARRDELEGILEEVSKRLEIEEQKAKKADSESRKLTEMVRHLEENLEDEERSRQKLLLEKNSIESRLKELEAQGLELEDSGNKLSKEKKALEERCEDLSSRLIDEVERSKQLVKAKARLEATVAEINDELEKEKQQRHNAETARRAAETQLREEQESCLEKTRKAEELTNQLMRKESELSQISIRNDEELAARQQLEREIREIRAQLDDAIEETNKEKAARQKAEKARRDMAEELESYKQELEESNDKTVLHSQLKAKRDEEYAHLQKQLEETVKSSEEVVEEMKAQNQKKIEELNETIDQLKRQKISADKAKSSAESDNENFRAELSNIASARLEAEKKRKAAETSLMEKDHKMREMQSNLDDLMAKLSKMNNELESIQKAKSADETLNSNLLKKNASLDMQLSELTEASEEDRRTRATLNNKIRQLEEDLAVAVEARDDALDAQEKIEKEVKEVKSLLAEARKKLDEENREVMEELRKKKEKELSAEKERADMAEQARDKAERAKKKAIQEAEDVQKELTDVVAATREMERKMRKFDQQLAEERNNTLLAQQERDMAHQMLRDAETKALVLSNELSEKKDIVDQLEKDKRTLKLEIDNLASTKDDAGKNVYELEKTKRRLDEELSRAEQQIIELEDALQLADDARSRVEVNMQAMRSEFERQLASREEDEDDRKKGLTSKIRNLTEELESEQRARQAAIANKKKIESQISELTEKNEASLRQIEDLSRQLRKAQLGWKDLQLDVTEARAAMEDALAGQRDAEKRARASEDEIKRLTADIQAVSSSKRKAEAERDELIEEVSSLRASSFSNEEKRRLEAKVIDLEDQLDEEASANELAQEKVRKSQQQLEQMTADLAMERSVCERTESDKIALERANRDLKQQLQDAENTAVARLRTQINVAEAKVSSLEQQLSLEEQDKMRQGRTLRRMETKMAEMQQMLEEEKRQGESNRQAVDRQNARIRQLRTQLEDTEAERDRLTNKLKDERRRAEEMTDLNETLSRDVSLLKQRETTARRTPGLIGHRESRRFGSNTSLARDEFRGSALTNEMSPSDRPASRLTSGTGSQLGNTDEDRDSVRN

Target ORF information:

RefSeq Version	NM_059785.7
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Non-muscle MYosin (nmy-2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_059785.7

ORF Insert Sequence:

ATGACATCAT CTCGACAAAA AGATGATGAG ATTGACCAAT TGAGAGCCAA GACAGACTTC 
CTCAATCCAT CGATTTCGGC AGATTGGGCG AAAAAAAAGT TGATGTGGGT ACCCAGTGAG 
AAGGATGGAT TCGCACTTGG AGCTGTCATC GGGGAACCAC ATGCAGACGG AACTATCGAC 
ATTGAGCTTA TGGAGACTGG CGAACGGCAG CGTGTTTCGT CTGATGATTG CCAGAAGCCA 
AATCCTCCGA AATATGACAA ATGCGAGGAT ATGTCAATGC TGACGTGTCT GAATGAAGCG 
TCGGTTCTTC ATAATCTAAA GCAACGATAT TTCTCGAATC TCTACTACAC ATACTCTGGC 
TTGTTCTGCG TTGTAATAAA TCCATACAAA CGAATTCCAA TCTACACGGA TACAATTGCT 
GAACAGTTCA AATGCAAAAA GAGAAAGGAG ATGCCACCAC ACATATTTGC TGTTGCCGAT 
GAAGCCTACC GCAGCATGCT TCAAGAACGA GATGATCAGT CAATTCTCTG CACTGGAGAA 
TCGGGAGCTG GAAAGACTGA AAATACAAAG AAAGTGATTC AGTATTTGGC TTATGTGGCA 
AATCGGTCGA TGGTGAAGAA TCGGAAGACT TCCGTTGATC TGGACACATC TACAAACCGG 
ATCATGGGCC AGCTTGAGGA ACAGCTTTTG CAAGCAAATC CCATACTCGA GGCTTTCGGA 
AACAGTAAGA CAGTGAAGAA CGATAATTCC AGTAGATTTG GAAAGTTCAT TCGCGTTCAT 
TTCGACAGCA CAGGGTGCAT TTCTGGAGCG AATATTGAAT TTTATCTTCT GGAGAAATCT 
CGCGTGCTGA AACAAGCTCC CAATGAGCGA TCGTTCCACA TTTTCTACCA GTTGCTGAAA 
GGACTCTCAA AAGTACAGAG AGAACATTAT CTCCTAGAAG ACAGCCTATC CAAGTACAAG 
TTTGTCTCAA ATGGTGACAG TAAATTGGCT GGAGTTGATG ATGGAGCAGA GATGAAGGAA 
ACACTGAATG CCATGTCAAT TATGGGATTG AACGACGAAG AGATTGGAGG AATACTTAGA 
GTTGTCTCGG CAGTTATGCT CTTTGGAAAT CTTGAATTTT CTCACGAAAA CAAGAATAAC 
GATCAGGCTG TTCTGCTCAA TGACGCTGTT GCTCAAAAAA TCGCTTCACT TCTCGGAGTC 
AATGTTACCG AATTGATGCG TGCATTCTTG AAGCCAAAAA TCAAGGTTCA AAGGGATTTG 
GTCCATCGAG CTCAAAGCGT AGACCAAGTC AATTTCTCTG TGGGAGCCAT TGCGAAGGCC 
TCGTATGAGC GACTCTTCAG ATGGTTGGTT CATCGCCTCA ACAAATCTCT CGATCGGACC 
CGTCAACAAT CTGTCAGCTT TATCGGAATT CTTGATATTG CTGGTTTTGA AATTTTTGAA 
ACAAACTCAT TCGAGCAGCT ATGTATCAAC TACACTAACG AGAAACTTCA ACAACTCTTC 
AACAATACAA TGTTCGTTCG TGAACAGCAA GAATATCTTG ATGAAGGATT GGAGTGGAAG 
TTCTTGGATT TCGGCCTCAA TCTTCAACCG ACTATTGACT TGATTGATAA GCCTATGGGA 
ATCATGTCAA CTCTTGATGA TGTTTGTCTA TTCCCACAAG GAAATGATCA ATCATTTGTT 
CAACGCCTCA ATAACACTCA TTCTCAACAT CCGAAGTACG TCGTTCCAGA GATTCGCTCA 
CGAAGCGATT TTGCCGTTGT TCATTATGCG GGCCGAGTCG ACTATCAATC TGAAGGTTGG 
CGCGTGAAAA ACATGGATCC ATTGAATGAG AATGTGATTG ATGTGCTCAA AACTTCAAAG 
GAATCTCTTA TTGTCGACAT GTGGAAAGAT ATCGCTGATG TTTGCAGTTT GTCAGCTGCT 
GACAGTACTT CAGATACCGG AGTATTTGGA TCTCGTGTTC CAAAGAAGGG AATGTTCCGT 
ACTGTTTCTC AACTTTACAA AGAACAATTG GCTCGTCTTA TGAGTACTCT CAATAACACC 
AATCCTCACT TTGTTCGCTG CATCATTCCA AATCACGAAA AGAAACATGG AGTTCTCAAC 
GCTCATCTTG TTCTTGATCA ATTAAGATGC AACGGAGTGT TGGAAGGAAT TCGTATATGC 
CGTCAAGGAT TCCCTACGCG GCTCCCGTTC CAAGAATTCC GTCAACGCTA CGAGAAACTT 
CTTGCCCCTG ATGTCAACCC AGCTGGATTC ATGGACGGTA AAAACGCCGT TTATCGTATT 
GTTCAATATC TGGAAGTTGA CGCCAACCTC TTCCGTATTG GTCAATCTAA GATCTTCTTC 
CGTTCTGGAG TTATAGCTGA GTTTGAGGAG ATGCGTGATC AGAAGCTTTC CGCTCTTATT 
GAATCTTTCC AAGCTCAATG TCGTGGATGG CTAGGACGCC GAGTGATGGT ACGCAGACGA 
GAACAAGAAG TTGCTATCAA AATTCTTCAA AGAAATGGTT TGGCATGGAT GAGACTGCGT 
GAATGGCAAT GGTGGAGACT TCTGACCAAA GTGAAGCCAC TTCTCGAAGT TACCAACAAA 
GACGAATTAA TTGCTGAGCG AGAACAAGAA CTGAAAGTGA CAGCTGAAAA GCTTCGTAGA 
TCCGAGGTAT TCATCTCCGA TTACAAGCAA CAAATGGAAA AAATGGACGA GGAGCGGCTG 
GTTTTAAAGA CGAGACTCGA TGCTGAATCG TCTGAGAGAG CTGAAATATT TGAGGAACGT 
TCCCGAATGG CTGCTCGGAG AGACGAACTT GAAGGAATCC TTGAAGAAGT TTCGAAGAGA 
CTGGAGATCG AAGAGCAAAA GGCAAAGAAG GCTGATTCTG AATCCCGAAA ATTGACGGAG 
ATGGTTAGAC ATCTCGAAGA GAATCTTGAA GATGAAGAAA GAAGCCGTCA GAAATTGTTG 
CTCGAAAAAA ATTCAATTGA ATCTCGGTTG AAGGAACTGG AAGCACAAGG ACTCGAGCTT 
GAAGATTCTG GAAACAAGTT GTCGAAAGAA AAGAAGGCTT TGGAAGAACG CTGTGAAGAT 
CTTTCGTCAC GTTTAATTGA CGAGGTTGAA CGATCGAAAC AATTGGTCAA GGCAAAGGCT 
CGCCTGGAAG CTACGGTTGC AGAGATAAAC GATGAATTGG AAAAAGAGAA GCAGCAGAGA 
CATAATGCCG AAACAGCTCG TCGTGCTGCC GAAACACAGC TCCGCGAGGA ACAAGAATCA 
TGCCTGGAAA AGACTCGTAA AGCGGAAGAG TTGACAAATC AGCTGATGCG CAAAGAGTCG 
GAATTGTCGC AAATAAGCAT TCGGAACGAC GAAGAACTCG CAGCTCGGCA ACAGTTAGAA 
CGTGAAATTC GGGAAATTCG TGCACAGCTT GACGATGCAA TTGAGGAGAC AAACAAGGAG 
AAAGCTGCTC GTCAGAAAGC AGAGAAAGCT CGTCGAGATA TGGCTGAAGA ATTGGAATCC 
TACAAGCAAG AATTGGAAGA ATCCAATGAT AAAACCGTTC TCCACTCGCA ACTCAAAGCT 
AAACGAGATG AAGAGTATGC TCATTTGCAG AAACAACTTG AGGAAACAGT CAAATCTTCT 
GAAGAAGTTG TGGAGGAAAT GAAGGCTCAG AATCAGAAAA AGATTGAGGA GTTGAATGAG 
ACGATCGATC AGTTGAAACG TCAGAAAATT TCGGCTGACA AGGCAAAATC TTCTGCTGAA 
TCTGATAACG AGAACTTCCG TGCCGAGCTG TCCAACATAG CATCGGCTCG TCTGGAGGCG 
GAAAAGAAGC GGAAAGCGGC CGAGACGTCG TTGATGGAGA AGGATCATAA GATGCGTGAA 
ATGCAGTCAA ATCTTGATGA TCTTATGGCT AAGTTATCGA AGATGAACAA CGAGCTTGAA 
AGCATTCAAA AAGCAAAGAG TGCTGACGAA ACTCTGAATT CTAACCTTCT CAAGAAGAAT 
GCTTCACTCG ACATGCAGTT GTCTGAGCTT ACTGAAGCTT CTGAGGAAGA TCGTCGAACT 
AGAGCCACGT TAAACAATAA AATTCGTCAG CTCGAAGAAG ATCTTGCGGT TGCTGTTGAA 
GCCAGAGATG ACGCACTTGA CGCACAAGAA AAGATCGAAA AAGAGGTCAA AGAAGTCAAA 
TCTCTGTTGG CCGAGGCTAG AAAGAAGTTG GATGAGGAAA ATAGAGAGGT GATGGAAGAA 
TTGCGGAAGA AGAAGGAGAA GGAGTTGTCA GCCGAGAAAG AGCGGGCCGA CATGGCTGAA 
CAAGCAAGAG ATAAGGCTGA ACGGGCCAAG AAGAAGGCTA TCCAGGAGGC TGAGGACGTG 
CAAAAAGAGT TGACTGATGT GGTGGCCGCA ACTAGAGAGA TGGAGAGAAA AATGAGGAAG 
TTTGATCAAC AGCTCGCCGA GGAGAGAAAC AACACTTTGC TCGCTCAGCA AGAAAGAGAT 
ATGGCTCATC AAATGCTACG TGATGCAGAG ACGAAGGCTC TGGTGTTGTC AAATGAATTA 
TCCGAGAAAA AGGATATTGT GGATCAGTTA GAAAAGGACA AACGGACTCT CAAACTAGAG 
ATTGACAATT TGGCCTCCAC CAAGGATGAT GCTGGAAAGA ATGTTTACGA GTTGGAGAAG 
ACGAAACGTC GCCTTGATGA AGAGTTGAGT CGTGCGGAAC AACAAATCAT TGAACTAGAA 
GATGCTCTCC AACTTGCTGA TGATGCTAGA AGCAGAGTTG AAGTCAACAT GCAAGCAATG 
AGATCCGAGT TCGAACGTCA ATTAGCCTCT CGCGAAGAAG ATGAGGATGA TCGTAAGAAG 
GGACTCACTT CTAAAATCCG AAATCTTACC GAAGAGCTTG AATCTGAACA ACGTGCTCGT 
CAAGCTGCAA TTGCCAACAA GAAGAAGATC GAATCTCAAA TCAGCGAGTT GACTGAAAAG 
AACGAAGCTT CTTTGAGACA AATCGAAGAT CTTTCGCGTC AGCTTCGCAA AGCCCAACTG 
GGATGGAAAG ATCTCCAGCT GGATGTTACA GAAGCTCGTG CTGCCATGGA AGATGCTCTT 
GCTGGACAAA GAGACGCAGA GAAGAGAGCT CGGGCTTCGG AGGATGAGAT CAAGAGACTC 
ACCGCCGATA TTCAAGCTGT CAGCTCTTCA AAACGTAAAG CAGAAGCTGA GCGCGATGAA 
CTCATTGAAG AAGTTTCTAG TCTTCGCGCT TCCTCATTCA GTAATGAGGA GAAGAGACGT 
CTTGAAGCAA AGGTAATTGA TTTGGAAGAC CAACTCGACG AGGAGGCTTC GGCAAATGAA 
TTGGCACAAG AGAAGGTTCG CAAGTCACAA CAGCAACTAG AACAAATGAC AGCTGATCTT 
GCAATGGAAA GAAGTGTTTG TGAGAGAACA GAATCAGATA AAATTGCATT GGAACGTGCG 
AATCGTGATT TGAAGCAACA ACTTCAAGAT GCTGAAAATA CCGCTGTGGC TCGTCTTCGT 
ACACAAATCA ACGTTGCGGA AGCCAAAGTT TCCAGTCTTG AGCAACAGCT TTCATTGGAA 
GAGCAGGATA AGATGCGTCA AGGCAGAACC CTGCGACGAA TGGAAACGAA AATGGCAGAG 
ATGCAGCAAA TGCTCGAGGA AGAGAAGAGA CAAGGTGAAA GTAATCGTCA GGCTGTTGAT 
CGTCAGAATG CTAGAATCCG TCAACTTCGT ACACAGTTGG AAGATACTGA GGCAGAGCGA 
GACCGTTTGA CAAACAAGTT GAAGGACGAG CGGAGAAGAG CTGAAGAGAT GACTGATCTC 
AATGAGACAC TCTCCAGAGA TGTTTCTTTA TTGAAACAGC GTGAAACGAC AGCTCGTAGA 
ACACCTGGAT TGATTGGACA TCGTGAATCG AGAAGATTTG GAAGCAACAC CAGCCTTGCA 
CGAGATGAAT TCCGTGGATC AGCTCTCACA AACGAAATGT CACCATCTGA CCGTCCAGCT 
TCTCGTCTCA CATCTGGAAC CGGATCCCAA CTTGGCAACA CCGATGAAGA CCGCGACTCA 
GTTCGCAACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nmy-2 ( NM_059785.6 ) cDNA ORF clone, Caenorhabditis elegans -> NP_492186.3 Caenorhabditis elegans Non-muscle MYosin (nmy-2), partial mRNA.

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CloneID	OCa335303
Clone ID Related Accession (Same CDS sequence)	NM_059785.7 , NM_059785.6
Accession Version	NM_059785.6	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6012bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Non-muscle MYosin
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003279). On Jun 26, 2012 this sequence version replaced NM_059785.5. COMPLETENESS: incomplete on both ends.

ATGACATCAT CTCGACAAAA AGATGATGAG ATTGACCAAT TGAGAGCCAA GACAGACTTC 
CTCAATCCAT CGATTTCGGC AGATTGGGCG AAAAAAAAGT TGATGTGGGT ACCCAGTGAG 
AAGGATGGAT TCGCACTTGG AGCTGTCATC GGGGAACCAC ATGCAGACGG AACTATCGAC 
ATTGAGCTTA TGGAGACTGG CGAACGGCAG CGTGTTTCGT CTGATGATTG CCAGAAGCCA 
AATCCTCCGA AATATGACAA ATGCGAGGAT ATGTCAATGC TGACGTGTCT GAATGAAGCG 
TCGGTTCTTC ATAATCTAAA GCAACGATAT TTCTCGAATC TCTACTACAC ATACTCTGGC 
TTGTTCTGCG TTGTAATAAA TCCATACAAA CGAATTCCAA TCTACACGGA TACAATTGCT 
GAACAGTTCA AATGCAAAAA GAGAAAGGAG ATGCCACCAC ACATATTTGC TGTTGCCGAT 
GAAGCCTACC GCAGCATGCT TCAAGAACGA GATGATCAGT CAATTCTCTG CACTGGAGAA 
TCGGGAGCTG GAAAGACTGA AAATACAAAG AAAGTGATTC AGTATTTGGC TTATGTGGCA 
AATCGGTCGA TGGTGAAGAA TCGGAAGACT TCCGTTGATC TGGACACATC TACAAACCGG 
ATCATGGGCC AGCTTGAGGA ACAGCTTTTG CAAGCAAATC CCATACTCGA GGCTTTCGGA 
AACAGTAAGA CAGTGAAGAA CGATAATTCC AGTAGATTTG GAAAGTTCAT TCGCGTTCAT 
TTCGACAGCA CAGGGTGCAT TTCTGGAGCG AATATTGAAT TTTATCTTCT GGAGAAATCT 
CGCGTGCTGA AACAAGCTCC CAATGAGCGA TCGTTCCACA TTTTCTACCA GTTGCTGAAA 
GGACTCTCAA AAGTACAGAG AGAACATTAT CTCCTAGAAG ACAGCCTATC CAAGTACAAG 
TTTGTCTCAA ATGGTGACAG TAAATTGGCT GGAGTTGATG ATGGAGCAGA GATGAAGGAA 
ACACTGAATG CCATGTCAAT TATGGGATTG AACGACGAAG AGATTGGAGG AATACTTAGA 
GTTGTCTCGG CAGTTATGCT CTTTGGAAAT CTTGAATTTT CTCACGAAAA CAAGAATAAC 
GATCAGGCTG TTCTGCTCAA TGACGCTGTT GCTCAAAAAA TCGCTTCACT TCTCGGAGTC 
AATGTTACCG AATTGATGCG TGCATTCTTG AAGCCAAAAA TCAAGGTTCA AAGGGATTTG 
GTCCATCGAG CTCAAAGCGT AGACCAAGTC AATTTCTCTG TGGGAGCCAT TGCGAAGGCC 
TCGTATGAGC GACTCTTCAG ATGGTTGGTT CATCGCCTCA ACAAATCTCT CGATCGGACC 
CGTCAACAAT CTGTCAGCTT TATCGGAATT CTTGATATTG CTGGTTTTGA AATTTTTGAA 
ACAAACTCAT TCGAGCAGCT ATGTATCAAC TACACTAACG AGAAACTTCA ACAACTCTTC 
AACAATACAA TGTTCGTTCG TGAACAGCAA GAATATCTTG ATGAAGGATT GGAGTGGAAG 
TTCTTGGATT TCGGCCTCAA TCTTCAACCG ACTATTGACT TGATTGATAA GCCTATGGGA 
ATCATGTCAA CTCTTGATGA TGTTTGTCTA TTCCCACAAG GAAATGATCA ATCATTTGTT 
CAACGCCTCA ATAACACTCA TTCTCAACAT CCGAAGTACG TCGTTCCAGA GATTCGCTCA 
CGAAGCGATT TTGCCGTTGT TCATTATGCG GGCCGAGTCG ACTATCAATC TGAAGGTTGG 
CGCGTGAAAA ACATGGATCC ATTGAATGAG AATGTGATTG ATGTGCTCAA AACTTCAAAG 
GAATCTCTTA TTGTCGACAT GTGGAAAGAT ATCGCTGATG TTTGCAGTTT GTCAGCTGCT 
GACAGTACTT CAGATACCGG AGTATTTGGA TCTCGTGTTC CAAAGAAGGG AATGTTCCGT 
ACTGTTTCTC AACTTTACAA AGAACAATTG GCTCGTCTTA TGAGTACTCT CAATAACACC 
AATCCTCACT TTGTTCGCTG CATCATTCCA AATCACGAAA AGAAACATGG AGTTCTCAAC 
GCTCATCTTG TTCTTGATCA ATTAAGATGC AACGGAGTGT TGGAAGGAAT TCGTATATGC 
CGTCAAGGAT TCCCTACGCG GCTCCCGTTC CAAGAATTCC GTCAACGCTA CGAGAAACTT 
CTTGCCCCTG ATGTCAACCC AGCTGGATTC ATGGACGGTA AAAACGCCGT TTATCGTATT 
GTTCAATATC TGGAAGTTGA CGCCAACCTC TTCCGTATTG GTCAATCTAA GATCTTCTTC 
CGTTCTGGAG TTATAGCTGA GTTTGAGGAG ATGCGTGATC AGAAGCTTTC CGCTCTTATT 
GAATCTTTCC AAGCTCAATG TCGTGGATGG CTAGGACGCC GAGTGATGGT ACGCAGACGA 
GAACAAGAAG TTGCTATCAA AATTCTTCAA AGAAATGGTT TGGCATGGAT GAGACTGCGT 
GAATGGCAAT GGTGGAGACT TCTGACCAAA GTGAAGCCAC TTCTCGAAGT TACCAACAAA 
GACGAATTAA TTGCTGAGCG AGAACAAGAA CTGAAAGTGA CAGCTGAAAA GCTTCGTAGA 
TCCGAGGTAT TCATCTCCGA TTACAAGCAA CAAATGGAAA AAATGGACGA GGAGCGGCTG 
GTTTTAAAGA CGAGACTCGA TGCTGAATCG TCTGAGAGAG CTGAAATATT TGAGGAACGT 
TCCCGAATGG CTGCTCGGAG AGACGAACTT GAAGGAATCC TTGAAGAAGT TTCGAAGAGA 
CTGGAGATCG AAGAGCAAAA GGCAAAGAAG GCTGATTCTG AATCCCGAAA ATTGACGGAG 
ATGGTTAGAC ATCTCGAAGA GAATCTTGAA GATGAAGAAA GAAGCCGTCA GAAATTGTTG 
CTCGAAAAAA ATTCAATTGA ATCTCGGTTG AAGGAACTGG AAGCACAAGG ACTCGAGCTT 
GAAGATTCTG GAAACAAGTT GTCGAAAGAA AAGAAGGCTT TGGAAGAACG CTGTGAAGAT 
CTTTCGTCAC GTTTAATTGA CGAGGTTGAA CGATCGAAAC AATTGGTCAA GGCAAAGGCT 
CGCCTGGAAG CTACGGTTGC AGAGATAAAC GATGAATTGG AAAAAGAGAA GCAGCAGAGA 
CATAATGCCG AAACAGCTCG TCGTGCTGCC GAAACACAGC TCCGCGAGGA ACAAGAATCA 
TGCCTGGAAA AGACTCGTAA AGCGGAAGAG TTGACAAATC AGCTGATGCG CAAAGAGTCG 
GAATTGTCGC AAATAAGCAT TCGGAACGAC GAAGAACTCG CAGCTCGGCA ACAGTTAGAA 
CGTGAAATTC GGGAAATTCG TGCACAGCTT GACGATGCAA TTGAGGAGAC AAACAAGGAG 
AAAGCTGCTC GTCAGAAAGC AGAGAAAGCT CGTCGAGATA TGGCTGAAGA ATTGGAATCC 
TACAAGCAAG AATTGGAAGA ATCCAATGAT AAAACCGTTC TCCACTCGCA ACTCAAAGCT 
AAACGAGATG AAGAGTATGC TCATTTGCAG AAACAACTTG AGGAAACAGT CAAATCTTCT 
GAAGAAGTTG TGGAGGAAAT GAAGGCTCAG AATCAGAAAA AGATTGAGGA GTTGAATGAG 
ACGATCGATC AGTTGAAACG TCAGAAAATT TCGGCTGACA AGGCAAAATC TTCTGCTGAA 
TCTGATAACG AGAACTTCCG TGCCGAGCTG TCCAACATAG CATCGGCTCG TCTGGAGGCG 
GAAAAGAAGC GGAAAGCGGC CGAGACGTCG TTGATGGAGA AGGATCATAA GATGCGTGAA 
ATGCAGTCAA ATCTTGATGA TCTTATGGCT AAGTTATCGA AGATGAACAA CGAGCTTGAA 
AGCATTCAAA AAGCAAAGAG TGCTGACGAA ACTCTGAATT CTAACCTTCT CAAGAAGAAT 
GCTTCACTCG ACATGCAGTT GTCTGAGCTT ACTGAAGCTT CTGAGGAAGA TCGTCGAACT 
AGAGCCACGT TAAACAATAA AATTCGTCAG CTCGAAGAAG ATCTTGCGGT TGCTGTTGAA 
GCCAGAGATG ACGCACTTGA CGCACAAGAA AAGATCGAAA AAGAGGTCAA AGAAGTCAAA 
TCTCTGTTGG CCGAGGCTAG AAAGAAGTTG GATGAGGAAA ATAGAGAGGT GATGGAAGAA 
TTGCGGAAGA AGAAGGAGAA GGAGTTGTCA GCCGAGAAAG AGCGGGCCGA CATGGCTGAA 
CAAGCAAGAG ATAAGGCTGA ACGGGCCAAG AAGAAGGCTA TCCAGGAGGC TGAGGACGTG 
CAAAAAGAGT TGACTGATGT GGTGGCCGCA ACTAGAGAGA TGGAGAGAAA AATGAGGAAG 
TTTGATCAAC AGCTCGCCGA GGAGAGAAAC AACACTTTGC TCGCTCAGCA AGAAAGAGAT 
ATGGCTCATC AAATGCTACG TGATGCAGAG ACGAAGGCTC TGGTGTTGTC AAATGAATTA 
TCCGAGAAAA AGGATATTGT GGATCAGTTA GAAAAGGACA AACGGACTCT CAAACTAGAG 
ATTGACAATT TGGCCTCCAC CAAGGATGAT GCTGGAAAGA ATGTTTACGA GTTGGAGAAG 
ACGAAACGTC GCCTTGATGA AGAGTTGAGT CGTGCGGAAC AACAAATCAT TGAACTAGAA 
GATGCTCTCC AACTTGCTGA TGATGCTAGA AGCAGAGTTG AAGTCAACAT GCAAGCAATG 
AGATCCGAGT TCGAACGTCA ATTAGCCTCT CGCGAAGAAG ATGAGGATGA TCGTAAGAAG 
GGACTCACTT CTAAAATCCG AAATCTTACC GAAGAGCTTG AATCTGAACA ACGTGCTCGT 
CAAGCTGCAA TTGCCAACAA GAAGAAGATC GAATCTCAAA TCAGCGAGTT GACTGAAAAG 
AACGAAGCTT CTTTGAGACA AATCGAAGAT CTTTCGCGTC AGCTTCGCAA AGCCCAACTG 
GGATGGAAAG ATCTCCAGCT GGATGTTACA GAAGCTCGTG CTGCCATGGA AGATGCTCTT 
GCTGGACAAA GAGACGCAGA GAAGAGAGCT CGGGCTTCGG AGGATGAGAT CAAGAGACTC 
ACCGCCGATA TTCAAGCTGT CAGCTCTTCA AAACGTAAAG CAGAAGCTGA GCGCGATGAA 
CTCATTGAAG AAGTTTCTAG TCTTCGCGCT TCCTCATTCA GTAATGAGGA GAAGAGACGT 
CTTGAAGCAA AGGTAATTGA TTTGGAAGAC CAACTCGACG AGGAGGCTTC GGCAAATGAA 
TTGGCACAAG AGAAGGTTCG CAAGTCACAA CAGCAACTAG AACAAATGAC AGCTGATCTT 
GCAATGGAAA GAAGTGTTTG TGAGAGAACA GAATCAGATA AAATTGCATT GGAACGTGCG 
AATCGTGATT TGAAGCAACA ACTTCAAGAT GCTGAAAATA CCGCTGTGGC TCGTCTTCGT 
ACACAAATCA ACGTTGCGGA AGCCAAAGTT TCCAGTCTTG AGCAACAGCT TTCATTGGAA 
GAGCAGGATA AGATGCGTCA AGGCAGAACC CTGCGACGAA TGGAAACGAA AATGGCAGAG 
ATGCAGCAAA TGCTCGAGGA AGAGAAGAGA CAAGGTGAAA GTAATCGTCA GGCTGTTGAT 
CGTCAGAATG CTAGAATCCG TCAACTTCGT ACACAGTTGG AAGATACTGA GGCAGAGCGA 
GACCGTTTGA CAAACAAGTT GAAGGACGAG CGGAGAAGAG CTGAAGAGAT GACTGATCTC 
AATGAGACAC TCTCCAGAGA TGTTTCTTTA TTGAAACAGC GTGAAACGAC AGCTCGTAGA 
ACACCTGGAT TGATTGGACA TCGTGAATCG AGAAGATTTG GAAGCAACAC CAGCCTTGCA 
CGAGATGAAT TCCGTGGATC AGCTCTCACA AACGAAATGT CACCATCTGA CCGTCCAGCT 
TCTCGTCTCA CATCTGGAAC CGGATCCCAA CTTGGCAACA CCGATGAAGA CCGCGACTCA 
GTTCGCAACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_492186.3
CDS	1..6012
Translation	MTSSRQKDDEIDQLRAKTDFLNPSISADWAKKKLMWVPSEKDGFALGAVIGEPHADGTIDIELMETGERQRVSSDDCQKPNPPKYDKCEDMSMLTCLNEASVLHNLKQRYFSNLYYTYSGLFCVVINPYKRIPIYTDTIAEQFKCKKRKEMPPHIFAVADEAYRSMLQERDDQSILCTGESGAGKTENTKKVIQYLAYVANRSMVKNRKTSVDLDTSTNRIMGQLEEQLLQANPILEAFGNSKTVKNDNSSRFGKFIRVHFDSTGCISGANIEFYLLEKSRVLKQAPNERSFHIFYQLLKGLSKVQREHYLLEDSLSKYKFVSNGDSKLAGVDDGAEMKETLNAMSIMGLNDEEIGGILRVVSAVMLFGNLEFSHENKNNDQAVLLNDAVAQKIASLLGVNVTELMRAFLKPKIKVQRDLVHRAQSVDQVNFSVGAIAKASYERLFRWLVHRLNKSLDRTRQQSVSFIGILDIAGFEIFETNSFEQLCINYTNEKLQQLFNNTMFVREQQEYLDEGLEWKFLDFGLNLQPTIDLIDKPMGIMSTLDDVCLFPQGNDQSFVQRLNNTHSQHPKYVVPEIRSRSDFAVVHYAGRVDYQSEGWRVKNMDPLNENVIDVLKTSKESLIVDMWKDIADVCSLSAADSTSDTGVFGSRVPKKGMFRTVSQLYKEQLARLMSTLNNTNPHFVRCIIPNHEKKHGVLNAHLVLDQLRCNGVLEGIRICRQGFPTRLPFQEFRQRYEKLLAPDVNPAGFMDGKNAVYRIVQYLEVDANLFRIGQSKIFFRSGVIAEFEEMRDQKLSALIESFQAQCRGWLGRRVMVRRREQEVAIKILQRNGLAWMRLREWQWWRLLTKVKPLLEVTNKDELIAEREQELKVTAEKLRRSEVFISDYKQQMEKMDEERLVLKTRLDAESSERAEIFEERSRMAARRDELEGILEEVSKRLEIEEQKAKKADSESRKLTEMVRHLEENLEDEERSRQKLLLEKNSIESRLKELEAQGLELEDSGNKLSKEKKALEERCEDLSSRLIDEVERSKQLVKAKARLEATVAEINDELEKEKQQRHNAETARRAAETQLREEQESCLEKTRKAEELTNQLMRKESELSQISIRNDEELAARQQLEREIREIRAQLDDAIEETNKEKAARQKAEKARRDMAEELESYKQELEESNDKTVLHSQLKAKRDEEYAHLQKQLEETVKSSEEVVEEMKAQNQKKIEELNETIDQLKRQKISADKAKSSAESDNENFRAELSNIASARLEAEKKRKAAETSLMEKDHKMREMQSNLDDLMAKLSKMNNELESIQKAKSADETLNSNLLKKNASLDMQLSELTEASEEDRRTRATLNNKIRQLEEDLAVAVEARDDALDAQEKIEKEVKEVKSLLAEARKKLDEENREVMEELRKKKEKELSAEKERADMAEQARDKAERAKKKAIQEAEDVQKELTDVVAATREMERKMRKFDQQLAEERNNTLLAQQERDMAHQMLRDAETKALVLSNELSEKKDIVDQLEKDKRTLKLEIDNLASTKDDAGKNVYELEKTKRRLDEELSRAEQQIIELEDALQLADDARSRVEVNMQAMRSEFERQLASREEDEDDRKKGLTSKIRNLTEELESEQRARQAAIANKKKIESQISELTEKNEASLRQIEDLSRQLRKAQLGWKDLQLDVTEARAAMEDALAGQRDAEKRARASEDEIKRLTADIQAVSSSKRKAEAERDELIEEVSSLRASSFSNEEKRRLEAKVIDLEDQLDEEASANELAQEKVRKSQQQLEQMTADLAMERSVCERTESDKIALERANRDLKQQLQDAENTAVARLRTQINVAEAKVSSLEQQLSLEEQDKMRQGRTLRRMETKMAEMQQMLEEEKRQGESNRQAVDRQNARIRQLRTQLEDTEAERDRLTNKLKDERRRAEEMTDLNETLSRDVSLLKQRETTARRTPGLIGHRESRRFGSNTSLARDEFRGSALTNEMSPSDRPASRLTSGTGSQLGNTDEDRDSVRN

Target ORF information:

RefSeq Version	NM_059785.6
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Non-muscle MYosin (nmy-2), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_059785.6

ORF Insert Sequence:

ATGACATCAT CTCGACAAAA AGATGATGAG ATTGACCAAT TGAGAGCCAA GACAGACTTC 
CTCAATCCAT CGATTTCGGC AGATTGGGCG AAAAAAAAGT TGATGTGGGT ACCCAGTGAG 
AAGGATGGAT TCGCACTTGG AGCTGTCATC GGGGAACCAC ATGCAGACGG AACTATCGAC 
ATTGAGCTTA TGGAGACTGG CGAACGGCAG CGTGTTTCGT CTGATGATTG CCAGAAGCCA 
AATCCTCCGA AATATGACAA ATGCGAGGAT ATGTCAATGC TGACGTGTCT GAATGAAGCG 
TCGGTTCTTC ATAATCTAAA GCAACGATAT TTCTCGAATC TCTACTACAC ATACTCTGGC 
TTGTTCTGCG TTGTAATAAA TCCATACAAA CGAATTCCAA TCTACACGGA TACAATTGCT 
GAACAGTTCA AATGCAAAAA GAGAAAGGAG ATGCCACCAC ACATATTTGC TGTTGCCGAT 
GAAGCCTACC GCAGCATGCT TCAAGAACGA GATGATCAGT CAATTCTCTG CACTGGAGAA 
TCGGGAGCTG GAAAGACTGA AAATACAAAG AAAGTGATTC AGTATTTGGC TTATGTGGCA 
AATCGGTCGA TGGTGAAGAA TCGGAAGACT TCCGTTGATC TGGACACATC TACAAACCGG 
ATCATGGGCC AGCTTGAGGA ACAGCTTTTG CAAGCAAATC CCATACTCGA GGCTTTCGGA 
AACAGTAAGA CAGTGAAGAA CGATAATTCC AGTAGATTTG GAAAGTTCAT TCGCGTTCAT 
TTCGACAGCA CAGGGTGCAT TTCTGGAGCG AATATTGAAT TTTATCTTCT GGAGAAATCT 
CGCGTGCTGA AACAAGCTCC CAATGAGCGA TCGTTCCACA TTTTCTACCA GTTGCTGAAA 
GGACTCTCAA AAGTACAGAG AGAACATTAT CTCCTAGAAG ACAGCCTATC CAAGTACAAG 
TTTGTCTCAA ATGGTGACAG TAAATTGGCT GGAGTTGATG ATGGAGCAGA GATGAAGGAA 
ACACTGAATG CCATGTCAAT TATGGGATTG AACGACGAAG AGATTGGAGG AATACTTAGA 
GTTGTCTCGG CAGTTATGCT CTTTGGAAAT CTTGAATTTT CTCACGAAAA CAAGAATAAC 
GATCAGGCTG TTCTGCTCAA TGACGCTGTT GCTCAAAAAA TCGCTTCACT TCTCGGAGTC 
AATGTTACCG AATTGATGCG TGCATTCTTG AAGCCAAAAA TCAAGGTTCA AAGGGATTTG 
GTCCATCGAG CTCAAAGCGT AGACCAAGTC AATTTCTCTG TGGGAGCCAT TGCGAAGGCC 
TCGTATGAGC GACTCTTCAG ATGGTTGGTT CATCGCCTCA ACAAATCTCT CGATCGGACC 
CGTCAACAAT CTGTCAGCTT TATCGGAATT CTTGATATTG CTGGTTTTGA AATTTTTGAA 
ACAAACTCAT TCGAGCAGCT ATGTATCAAC TACACTAACG AGAAACTTCA ACAACTCTTC 
AACAATACAA TGTTCGTTCG TGAACAGCAA GAATATCTTG ATGAAGGATT GGAGTGGAAG 
TTCTTGGATT TCGGCCTCAA TCTTCAACCG ACTATTGACT TGATTGATAA GCCTATGGGA 
ATCATGTCAA CTCTTGATGA TGTTTGTCTA TTCCCACAAG GAAATGATCA ATCATTTGTT 
CAACGCCTCA ATAACACTCA TTCTCAACAT CCGAAGTACG TCGTTCCAGA GATTCGCTCA 
CGAAGCGATT TTGCCGTTGT TCATTATGCG GGCCGAGTCG ACTATCAATC TGAAGGTTGG 
CGCGTGAAAA ACATGGATCC ATTGAATGAG AATGTGATTG ATGTGCTCAA AACTTCAAAG 
GAATCTCTTA TTGTCGACAT GTGGAAAGAT ATCGCTGATG TTTGCAGTTT GTCAGCTGCT 
GACAGTACTT CAGATACCGG AGTATTTGGA TCTCGTGTTC CAAAGAAGGG AATGTTCCGT 
ACTGTTTCTC AACTTTACAA AGAACAATTG GCTCGTCTTA TGAGTACTCT CAATAACACC 
AATCCTCACT TTGTTCGCTG CATCATTCCA AATCACGAAA AGAAACATGG AGTTCTCAAC 
GCTCATCTTG TTCTTGATCA ATTAAGATGC AACGGAGTGT TGGAAGGAAT TCGTATATGC 
CGTCAAGGAT TCCCTACGCG GCTCCCGTTC CAAGAATTCC GTCAACGCTA CGAGAAACTT 
CTTGCCCCTG ATGTCAACCC AGCTGGATTC ATGGACGGTA AAAACGCCGT TTATCGTATT 
GTTCAATATC TGGAAGTTGA CGCCAACCTC TTCCGTATTG GTCAATCTAA GATCTTCTTC 
CGTTCTGGAG TTATAGCTGA GTTTGAGGAG ATGCGTGATC AGAAGCTTTC CGCTCTTATT 
GAATCTTTCC AAGCTCAATG TCGTGGATGG CTAGGACGCC GAGTGATGGT ACGCAGACGA 
GAACAAGAAG TTGCTATCAA AATTCTTCAA AGAAATGGTT TGGCATGGAT GAGACTGCGT 
GAATGGCAAT GGTGGAGACT TCTGACCAAA GTGAAGCCAC TTCTCGAAGT TACCAACAAA 
GACGAATTAA TTGCTGAGCG AGAACAAGAA CTGAAAGTGA CAGCTGAAAA GCTTCGTAGA 
TCCGAGGTAT TCATCTCCGA TTACAAGCAA CAAATGGAAA AAATGGACGA GGAGCGGCTG 
GTTTTAAAGA CGAGACTCGA TGCTGAATCG TCTGAGAGAG CTGAAATATT TGAGGAACGT 
TCCCGAATGG CTGCTCGGAG AGACGAACTT GAAGGAATCC TTGAAGAAGT TTCGAAGAGA 
CTGGAGATCG AAGAGCAAAA GGCAAAGAAG GCTGATTCTG AATCCCGAAA ATTGACGGAG 
ATGGTTAGAC ATCTCGAAGA GAATCTTGAA GATGAAGAAA GAAGCCGTCA GAAATTGTTG 
CTCGAAAAAA ATTCAATTGA ATCTCGGTTG AAGGAACTGG AAGCACAAGG ACTCGAGCTT 
GAAGATTCTG GAAACAAGTT GTCGAAAGAA AAGAAGGCTT TGGAAGAACG CTGTGAAGAT 
CTTTCGTCAC GTTTAATTGA CGAGGTTGAA CGATCGAAAC AATTGGTCAA GGCAAAGGCT 
CGCCTGGAAG CTACGGTTGC AGAGATAAAC GATGAATTGG AAAAAGAGAA GCAGCAGAGA 
CATAATGCCG AAACAGCTCG TCGTGCTGCC GAAACACAGC TCCGCGAGGA ACAAGAATCA 
TGCCTGGAAA AGACTCGTAA AGCGGAAGAG TTGACAAATC AGCTGATGCG CAAAGAGTCG 
GAATTGTCGC AAATAAGCAT TCGGAACGAC GAAGAACTCG CAGCTCGGCA ACAGTTAGAA 
CGTGAAATTC GGGAAATTCG TGCACAGCTT GACGATGCAA TTGAGGAGAC AAACAAGGAG 
AAAGCTGCTC GTCAGAAAGC AGAGAAAGCT CGTCGAGATA TGGCTGAAGA ATTGGAATCC 
TACAAGCAAG AATTGGAAGA ATCCAATGAT AAAACCGTTC TCCACTCGCA ACTCAAAGCT 
AAACGAGATG AAGAGTATGC TCATTTGCAG AAACAACTTG AGGAAACAGT CAAATCTTCT 
GAAGAAGTTG TGGAGGAAAT GAAGGCTCAG AATCAGAAAA AGATTGAGGA GTTGAATGAG 
ACGATCGATC AGTTGAAACG TCAGAAAATT TCGGCTGACA AGGCAAAATC TTCTGCTGAA 
TCTGATAACG AGAACTTCCG TGCCGAGCTG TCCAACATAG CATCGGCTCG TCTGGAGGCG 
GAAAAGAAGC GGAAAGCGGC CGAGACGTCG TTGATGGAGA AGGATCATAA GATGCGTGAA 
ATGCAGTCAA ATCTTGATGA TCTTATGGCT AAGTTATCGA AGATGAACAA CGAGCTTGAA 
AGCATTCAAA AAGCAAAGAG TGCTGACGAA ACTCTGAATT CTAACCTTCT CAAGAAGAAT 
GCTTCACTCG ACATGCAGTT GTCTGAGCTT ACTGAAGCTT CTGAGGAAGA TCGTCGAACT 
AGAGCCACGT TAAACAATAA AATTCGTCAG CTCGAAGAAG ATCTTGCGGT TGCTGTTGAA 
GCCAGAGATG ACGCACTTGA CGCACAAGAA AAGATCGAAA AAGAGGTCAA AGAAGTCAAA 
TCTCTGTTGG CCGAGGCTAG AAAGAAGTTG GATGAGGAAA ATAGAGAGGT GATGGAAGAA 
TTGCGGAAGA AGAAGGAGAA GGAGTTGTCA GCCGAGAAAG AGCGGGCCGA CATGGCTGAA 
CAAGCAAGAG ATAAGGCTGA ACGGGCCAAG AAGAAGGCTA TCCAGGAGGC TGAGGACGTG 
CAAAAAGAGT TGACTGATGT GGTGGCCGCA ACTAGAGAGA TGGAGAGAAA AATGAGGAAG 
TTTGATCAAC AGCTCGCCGA GGAGAGAAAC AACACTTTGC TCGCTCAGCA AGAAAGAGAT 
ATGGCTCATC AAATGCTACG TGATGCAGAG ACGAAGGCTC TGGTGTTGTC AAATGAATTA 
TCCGAGAAAA AGGATATTGT GGATCAGTTA GAAAAGGACA AACGGACTCT CAAACTAGAG 
ATTGACAATT TGGCCTCCAC CAAGGATGAT GCTGGAAAGA ATGTTTACGA GTTGGAGAAG 
ACGAAACGTC GCCTTGATGA AGAGTTGAGT CGTGCGGAAC AACAAATCAT TGAACTAGAA 
GATGCTCTCC AACTTGCTGA TGATGCTAGA AGCAGAGTTG AAGTCAACAT GCAAGCAATG 
AGATCCGAGT TCGAACGTCA ATTAGCCTCT CGCGAAGAAG ATGAGGATGA TCGTAAGAAG 
GGACTCACTT CTAAAATCCG AAATCTTACC GAAGAGCTTG AATCTGAACA ACGTGCTCGT 
CAAGCTGCAA TTGCCAACAA GAAGAAGATC GAATCTCAAA TCAGCGAGTT GACTGAAAAG 
AACGAAGCTT CTTTGAGACA AATCGAAGAT CTTTCGCGTC AGCTTCGCAA AGCCCAACTG 
GGATGGAAAG ATCTCCAGCT GGATGTTACA GAAGCTCGTG CTGCCATGGA AGATGCTCTT 
GCTGGACAAA GAGACGCAGA GAAGAGAGCT CGGGCTTCGG AGGATGAGAT CAAGAGACTC 
ACCGCCGATA TTCAAGCTGT CAGCTCTTCA AAACGTAAAG CAGAAGCTGA GCGCGATGAA 
CTCATTGAAG AAGTTTCTAG TCTTCGCGCT TCCTCATTCA GTAATGAGGA GAAGAGACGT 
CTTGAAGCAA AGGTAATTGA TTTGGAAGAC CAACTCGACG AGGAGGCTTC GGCAAATGAA 
TTGGCACAAG AGAAGGTTCG CAAGTCACAA CAGCAACTAG AACAAATGAC AGCTGATCTT 
GCAATGGAAA GAAGTGTTTG TGAGAGAACA GAATCAGATA AAATTGCATT GGAACGTGCG 
AATCGTGATT TGAAGCAACA ACTTCAAGAT GCTGAAAATA CCGCTGTGGC TCGTCTTCGT 
ACACAAATCA ACGTTGCGGA AGCCAAAGTT TCCAGTCTTG AGCAACAGCT TTCATTGGAA 
GAGCAGGATA AGATGCGTCA AGGCAGAACC CTGCGACGAA TGGAAACGAA AATGGCAGAG 
ATGCAGCAAA TGCTCGAGGA AGAGAAGAGA CAAGGTGAAA GTAATCGTCA GGCTGTTGAT 
CGTCAGAATG CTAGAATCCG TCAACTTCGT ACACAGTTGG AAGATACTGA GGCAGAGCGA 
GACCGTTTGA CAAACAAGTT GAAGGACGAG CGGAGAAGAG CTGAAGAGAT GACTGATCTC 
AATGAGACAC TCTCCAGAGA TGTTTCTTTA TTGAAACAGC GTGAAACGAC AGCTCGTAGA 
ACACCTGGAT TGATTGGACA TCGTGAATCG AGAAGATTTG GAAGCAACAC CAGCCTTGCA 
CGAGATGAAT TCCGTGGATC AGCTCTCACA AACGAAATGT CACCATCTGA CCGTCCAGCT 
TCTCGTCTCA CATCTGGAAC CGGATCCCAA CTTGGCAACA CCGATGAAGA CCGCGACTCA 
GTTCGCAACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.
Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

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nmy-2 cDNA ORF clone, Caenorhabditis elegans

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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