sep-1 cDNA ORF clone, Caenorhabditis elegans

Gene
Clones
gRNAs

Summary

Gene Symbol	sep-1
Entrez Gene ID	171912
Full Name	Separin homolog sep-1
Gene Type	protein-coding
Organism	Caenorhabditis elegans

mRNA and Protein(s)

mRNA	Protein	Name
NM_058759.3	NP_491160.1	Separin homolog sep-1
NM_058759.4	NP_491160.1	Separin homolog sep-1

Pathways from BioSystems

Homologies

Caenorhabditis elegans (roundworm)	ZK430.5	NP_494778.1
Caenorhabditis elegans (roundworm)	sep-1	NP_491160.1

Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.

Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

single-particle cryo-electron microscopy (cryo-EM) was used to determine a near-atomic-resolution structure of the Caenorhabditis elegans separase-securin complex.
Title: Cryo-EM structure of a metazoan separase-securin complex at near-atomic resolution.

Examination of the N-terminal tail sequence of CPAR-1 revealed a putative separase cleavage site and mutation of the signature residues in this site eliminated the cleavage reaction.
Title: Separase Cleaves the N-Tail of the CENP-A Related Protein CPAR-1 at the Meiosis I Metaphase-Anaphase Transition in C. elegans.

separase is required for cytokinesis by regulating the incorporation of RAB-11-positive vesicles into the plasma membrane at the cleavage furrow and midbody
Title: A role for separase in the regulation of RAB-11-positive vesicles at the cleavage furrow and midbody.

SEP-1 has two separable yet coordinated functions: to regulate cortical granule exocytosis and to mediate chromosome separation
Title: Cortical granule exocytosis in C. elegans is regulated by cell cycle components including separase.

The following sep-1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the sep-1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa337089	NM_058759.4 Latest version!	Caenorhabditis elegans Separin homolog sep-1 (sep-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
View Old Accession Versions >>
OCa337089	NM_058759.3	Caenorhabditis elegans Separin homolog sep-1 (sep-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

sep-1 ( NM_058759.3 ) cDNA ORF clone, Caenorhabditis elegans -> NP_491160.1 Caenorhabditis elegans Separin homolog sep-1 (sep-1), partial mRNA.

Price & Availability↑

CloneID	OCa337089
Clone ID Related Accession (Same CDS sequence)	NM_058759.3 , NM_058759.4
Accession Version	NM_058759.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3789bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Separin homolog sep-1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003279). On Jun 26, 2012 this sequence version replaced NM_058759.2. COMPLETENESS: incomplete on both ends.

ATGAAGATCA CAAACAAGTC AGTTGACAAG CAGCACATCG AAAAACTAGA TGAGCTACGC 
AAAAATGTGA GCTGCACGGT GATCGGGTTT GCCGAACAAA CTGCCGAGTT GCAACAGGAG 
ATTTCCGAGC TTTTTATCGC CGAATTCGGC GTCAATGGAC CGATTGACAT GAACTCGCTG 
AGCAAATTGG CTCGAATCAC CTCATACTAC GCCTCGTCCG AATACTTTCA AGGACTTGCG 
AAGTATCAAC GAACCGCATG CAAAATGTTC ATCACGTGGC AAACTCTACG AAAGGAGGCG 
ATGGAGTGTC GGAGCAAAGA TCGAGAGATT TTCGCGAGTA TTCCCGCGAA ACTCTGCTTT 
TTCTACTTTT ACAACGGAGA ACTGTGCAGA GCAGTCGTGT GCCTGCTCGA CTACATCGAC 
CTATCAGACG ACACTCTGGC CAAGGAAGCT GCTCTTCGAT GGTTGATGTT TCTCGGCGAG 
ACCGAGCTAA TTGAGAAGAA GCTGAAGACG TGGAAAATGG ATAAAAGCTC GAAAGACATG 
TTCTCGGCTA CCGAATTCGC GATGAACTAC CTGAAAAAGT CAGAGTATCG CGTGGAAATG 
CTCGAAAAGC TGATGAAGCT TCGCGATAAA GTCAAATCGG ATCCTACTCG CTCATTTTCG 
AGATACGAGT TGGCTTCTTA TGTCTCTTGG TTGTGCTCAA CGTTGAGCAA TGTTCCCGTT 
GGATCCGCAC TCAGGGAGTG TGAGTTCCCG GATCGTGTGT CCCATATTCA AGAAGCAGCA 
TTAAAAAGTG ATTCACTTGT GCGTAATCGA ATTCCTGGCC TCGCCAGCTC TCAATTCGAC 
AACTCGGTTA ATGCATCAAT TTGGCCATTT CTAGACGGGC ATCAAGAAGA TAGCAATTAT 
TATGTGCACA TTGGCAGTAC AATCGCTTGG CACTTTGAGA TGCGTCGGGA GTGTGCTCTC 
GTCAACGTCA CCACCGCCCA AACTCGTGAT TCGATGTCGG CAATGATTCT GAATCTTCGT 
GTCGCATTAA AATCGGCTTC ATTCTTTCGA GTTCTTCAGA CAACCAACAC ATTAGCCTAC 
TATTCGAGTA TCATCGAAGA AGCCGGAAGT GAGAAGAACG CGAAATTAAT GAGAGTATCG 
TGTGTTAATT TGCTGTCGTC TAATCCGATA ATTGTTCGGT GTAGCACTCC GAAGGAGACG 
GGAGCCACTT CGAGAGCTCA TACACCGATG GCAGGGAGTA GTGTATCGGA GAAACAGAAC 
ACGATGAGAC CTGATCTGGC TGATCTGTTG GGAGATCTCG AATTACTCGA TGAACAATCA 
TTCCATCCGA TCACCCGATC CTGCGTCTGC AATGTCTGCA CGATTTACCC GCTGCATAGC 
TCATTCGCCG CCGAATACAT GATGTCCTAC GCAATTCACT CGGATTTCTC GCAACTCTCC 
ATCAAACATT TCAACGACGA GTTCGCGAGA ATCAGAGAGC GTGGAATGTC TAGTCAGGTT 
CTGATGCATC GGGACTCGAG TGTGAGGCCG AGACCGAATA TCATTCAAAA CGAGATTTTC 
GGCATGTGTG TGATCCGGTG GCTGACGAAA AAGTTGGATT CGAAAGAAAG TGCTGACGAG 
GATACGATGG AGATTTTCAA CAACGCGTTG AAGATCGTGA GATATCTTCA ACAACGAACA 
ACCGATATGA TTCTCGCAGT GACACAACTT GGACGACAAC TCGAGTTTCC CATGGAATGC 
AACTATAGCT GGATGCGGCC AACGATCCGG AAACCACGTG TCAAGGCAAC GATCGATTGT 
GCCGTCGACA TTCTTCGCGC GGTTTCTCCG TTCGGACGTC GTCCGAAAGT TGAGAAACTG 
GAGAAGAATC TTCAGCCTTT CGATAAGGAA CGCTTCGAGA AGGTTCGGCT TGCCATGCGG 
AACGAGATGA ACCACTACGG CCATATTCTA TACAGAGAAT GGCGTTGCCG CTTGTTCGCC 
TACGTCGGAA GAACATCACG TGATCCTTGG GAGGCTGCGT ACGCATGGGC CGAGTCAACA 
CAGATCGGAG CTCGTAACGC TGTACAGAGC CGACTTGAGA AGTGCAAGAG AGGATTGGTG 
ACAATGTCGG GACATGATCG CTTCAAAACG TGTGTACAGT CGATGCCAGA CGAGATGACG 
CTTGTACAGA TTGCGATGGC CGATGATAAG ACGATTTATT TGGTGAAACT GCACGCGGAT 
CGTGATCCGA TCATCATGCC ACTGGCACAC TACTCTCAAG CCGTCGAATT GATGGATAAA 
TTCACGTTTT TGTTGGATGA AGACGAGATG ATAGCAAAGT ATCCCGGAGA CATTACACCA 
GAAGAGTTTT GGAAACGACG GAAAATTGTG GATGGTAGAA TGATGACGTT TGTTGACGAG 
GTTCAGAAGC ATTTCCTAGG TGTTGCGGCG AGTCTTCTGA TGCCGTCTGG ACAGTTGGGC 
CCGAAAGCGG CAGAACTTGC AATTAAAATC CACAAGTTGT CGAAAGGAGG ACTCCTTCTG 
GGAGAGGCCA AGGAGATGGT ATATCAGTCA AAGTTGATGG ATGCGAAATC CTGGGAAGCG 
CTGATTCTTC GATTCTGTGA GATGAGAACT ACCGATGAGA AGTTCAAATC GTTCCTGCCG 
CTGATGCACA GGAATTCGGT TGAAGTAATG AATCAGGATG ATTCGATTGT TACGGAAAAG 
AAGTACACGT ACCTTGTCAT CTGTCCCCAT CTTTCTCAAT TCTGTTGGGA GCGATTGCCG 
ATTTTCGATG AGTATCCATA TGTGGGACGT CAAGTATCGA TTCACTCGAC TTTTAGTCAG 
TTGGAAGCAA TGAAAAGTCA GGAGAAACAG ATTCCTCTAC AAATCGACGT ACAAAACGCC 
TACTACATTC TCGATCCTGA CAACAATTTG GGCGAAACAC AAAAACGAAT GGTCGAATAT 
ATCAATAAGT TCAATTGGGA AGGAACTGTT GGCTCGGCGC CAAAATCGAA TGAGATTAGC 
GCCGCGTTGT CTCAACGCGA TGCGTTTTTC TTTATTGGAC ATGGCTCTGG ATCTTCGGTT 
ATGCCACGAT CTGTGCTCAA GCAGTCGACG TGTAATGCGA TCAGTTTGCT GATGGGTTGT 
GGAAGTGTCC GAACAATTCC ACAAGCTCTC GGTTTTGATG GAAAAACGGC GATTCTGGAC 
TACGCGATGG CAAAGTGTCC ACTGATCGTT GGATGCTTAT GGACTGTCAC TGATGGTGAA 
ATCGATCGTT TCTTGATTCG AATGATCGAT GATTGTTTCG AAGATTCCAA ATCGCTCACC 
GGAATCGACA AGCTTCGTCA GCTATCGGAG GCTATGCACG AGGCCAGATC AAAAGCCAGA 
CTGAAATATT TGACCGGTGC CGCTGTGGTG ATGTATGGAT TGCCAGTTGT TGCAAAGCAA 
ACGACGCCAT TCGTGGAAAA GGATCAGAGA AACTTGCCGC AGACTCCGAA GACTTCAGCC 
CGAACTTCGA TGCGAATGGA AACTGTGCCG AAGACACCGA AACAAGAATT TGTTACATCG 
AAGTCTGTTC CAATGACTCC AATTTTCTCA AATAATGAGA ACAAGTCTCC AAGCCGAGCA 
AGGATGCCAA GCAGAGTTCT GAAGACACCT CGTCAGGTGA AAACGTTCCA AGAGGAAGAT 
GATGAAGCTC CGAAACGGTC GACAACGCGG CAGCTGAAAC CGCTGGTTGC ACCACCTATT 
CCAGCAACAC CGACGACTAG AACAACTCGC TCAAGTGCCC GAACTCCATC AAGATCCCGA 
AATTTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_491160.1
CDS	1..3789
Translation	MKITNKSVDKQHIEKLDELRKNVSCTVIGFAEQTAELQQEISELFIAEFGVNGPIDMNSLSKLARITSYYASSEYFQGLAKYQRTACKMFITWQTLRKEAMECRSKDREIFASIPAKLCFFYFYNGELCRAVVCLLDYIDLSDDTLAKEAALRWLMFLGETELIEKKLKTWKMDKSSKDMFSATEFAMNYLKKSEYRVEMLEKLMKLRDKVKSDPTRSFSRYELASYVSWLCSTLSNVPVGSALRECEFPDRVSHIQEAALKSDSLVRNRIPGLASSQFDNSVNASIWPFLDGHQEDSNYYVHIGSTIAWHFEMRRECALVNVTTAQTRDSMSAMILNLRVALKSASFFRVLQTTNTLAYYSSIIEEAGSEKNAKLMRVSCVNLLSSNPIIVRCSTPKETGATSRAHTPMAGSSVSEKQNTMRPDLADLLGDLELLDEQSFHPITRSCVCNVCTIYPLHSSFAAEYMMSYAIHSDFSQLSIKHFNDEFARIRERGMSSQVLMHRDSSVRPRPNIIQNEIFGMCVIRWLTKKLDSKESADEDTMEIFNNALKIVRYLQQRTTDMILAVTQLGRQLEFPMECNYSWMRPTIRKPRVKATIDCAVDILRAVSPFGRRPKVEKLEKNLQPFDKERFEKVRLAMRNEMNHYGHILYREWRCRLFAYVGRTSRDPWEAAYAWAESTQIGARNAVQSRLEKCKRGLVTMSGHDRFKTCVQSMPDEMTLVQIAMADDKTIYLVKLHADRDPIIMPLAHYSQAVELMDKFTFLLDEDEMIAKYPGDITPEEFWKRRKIVDGRMMTFVDEVQKHFLGVAASLLMPSGQLGPKAAELAIKIHKLSKGGLLLGEAKEMVYQSKLMDAKSWEALILRFCEMRTTDEKFKSFLPLMHRNSVEVMNQDDSIVTEKKYTYLVICPHLSQFCWERLPIFDEYPYVGRQVSIHSTFSQLEAMKSQEKQIPLQIDVQNAYYILDPDNNLGETQKRMVEYINKFNWEGTVGSAPKSNEISAALSQRDAFFFIGHGSGSSVMPRSVLKQSTCNAISLLMGCGSVRTIPQALGFDGKTAILDYAMAKCPLIVGCLWTVTDGEIDRFLIRMIDDCFEDSKSLTGIDKLRQLSEAMHEARSKARLKYLTGAAVVMYGLPVVAKQTTPFVEKDQRNLPQTPKTSARTSMRMETVPKTPKQEFVTSKSVPMTPIFSNNENKSPSRARMPSRVLKTPRQVKTFQEEDDEAPKRSTTRQLKPLVAPPIPATPTTRTTRSSARTPSRSRNL

Target ORF information:

RefSeq Version	NM_058759.3
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Separin homolog sep-1 (sep-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_058759.3

ORF Insert Sequence:

ATGAAGATCA CAAACAAGTC AGTTGACAAG CAGCACATCG AAAAACTAGA TGAGCTACGC 
AAAAATGTGA GCTGCACGGT GATCGGGTTT GCCGAACAAA CTGCCGAGTT GCAACAGGAG 
ATTTCCGAGC TTTTTATCGC CGAATTCGGC GTCAATGGAC CGATTGACAT GAACTCGCTG 
AGCAAATTGG CTCGAATCAC CTCATACTAC GCCTCGTCCG AATACTTTCA AGGACTTGCG 
AAGTATCAAC GAACCGCATG CAAAATGTTC ATCACGTGGC AAACTCTACG AAAGGAGGCG 
ATGGAGTGTC GGAGCAAAGA TCGAGAGATT TTCGCGAGTA TTCCCGCGAA ACTCTGCTTT 
TTCTACTTTT ACAACGGAGA ACTGTGCAGA GCAGTCGTGT GCCTGCTCGA CTACATCGAC 
CTATCAGACG ACACTCTGGC CAAGGAAGCT GCTCTTCGAT GGTTGATGTT TCTCGGCGAG 
ACCGAGCTAA TTGAGAAGAA GCTGAAGACG TGGAAAATGG ATAAAAGCTC GAAAGACATG 
TTCTCGGCTA CCGAATTCGC GATGAACTAC CTGAAAAAGT CAGAGTATCG CGTGGAAATG 
CTCGAAAAGC TGATGAAGCT TCGCGATAAA GTCAAATCGG ATCCTACTCG CTCATTTTCG 
AGATACGAGT TGGCTTCTTA TGTCTCTTGG TTGTGCTCAA CGTTGAGCAA TGTTCCCGTT 
GGATCCGCAC TCAGGGAGTG TGAGTTCCCG GATCGTGTGT CCCATATTCA AGAAGCAGCA 
TTAAAAAGTG ATTCACTTGT GCGTAATCGA ATTCCTGGCC TCGCCAGCTC TCAATTCGAC 
AACTCGGTTA ATGCATCAAT TTGGCCATTT CTAGACGGGC ATCAAGAAGA TAGCAATTAT 
TATGTGCACA TTGGCAGTAC AATCGCTTGG CACTTTGAGA TGCGTCGGGA GTGTGCTCTC 
GTCAACGTCA CCACCGCCCA AACTCGTGAT TCGATGTCGG CAATGATTCT GAATCTTCGT 
GTCGCATTAA AATCGGCTTC ATTCTTTCGA GTTCTTCAGA CAACCAACAC ATTAGCCTAC 
TATTCGAGTA TCATCGAAGA AGCCGGAAGT GAGAAGAACG CGAAATTAAT GAGAGTATCG 
TGTGTTAATT TGCTGTCGTC TAATCCGATA ATTGTTCGGT GTAGCACTCC GAAGGAGACG 
GGAGCCACTT CGAGAGCTCA TACACCGATG GCAGGGAGTA GTGTATCGGA GAAACAGAAC 
ACGATGAGAC CTGATCTGGC TGATCTGTTG GGAGATCTCG AATTACTCGA TGAACAATCA 
TTCCATCCGA TCACCCGATC CTGCGTCTGC AATGTCTGCA CGATTTACCC GCTGCATAGC 
TCATTCGCCG CCGAATACAT GATGTCCTAC GCAATTCACT CGGATTTCTC GCAACTCTCC 
ATCAAACATT TCAACGACGA GTTCGCGAGA ATCAGAGAGC GTGGAATGTC TAGTCAGGTT 
CTGATGCATC GGGACTCGAG TGTGAGGCCG AGACCGAATA TCATTCAAAA CGAGATTTTC 
GGCATGTGTG TGATCCGGTG GCTGACGAAA AAGTTGGATT CGAAAGAAAG TGCTGACGAG 
GATACGATGG AGATTTTCAA CAACGCGTTG AAGATCGTGA GATATCTTCA ACAACGAACA 
ACCGATATGA TTCTCGCAGT GACACAACTT GGACGACAAC TCGAGTTTCC CATGGAATGC 
AACTATAGCT GGATGCGGCC AACGATCCGG AAACCACGTG TCAAGGCAAC GATCGATTGT 
GCCGTCGACA TTCTTCGCGC GGTTTCTCCG TTCGGACGTC GTCCGAAAGT TGAGAAACTG 
GAGAAGAATC TTCAGCCTTT CGATAAGGAA CGCTTCGAGA AGGTTCGGCT TGCCATGCGG 
AACGAGATGA ACCACTACGG CCATATTCTA TACAGAGAAT GGCGTTGCCG CTTGTTCGCC 
TACGTCGGAA GAACATCACG TGATCCTTGG GAGGCTGCGT ACGCATGGGC CGAGTCAACA 
CAGATCGGAG CTCGTAACGC TGTACAGAGC CGACTTGAGA AGTGCAAGAG AGGATTGGTG 
ACAATGTCGG GACATGATCG CTTCAAAACG TGTGTACAGT CGATGCCAGA CGAGATGACG 
CTTGTACAGA TTGCGATGGC CGATGATAAG ACGATTTATT TGGTGAAACT GCACGCGGAT 
CGTGATCCGA TCATCATGCC ACTGGCACAC TACTCTCAAG CCGTCGAATT GATGGATAAA 
TTCACGTTTT TGTTGGATGA AGACGAGATG ATAGCAAAGT ATCCCGGAGA CATTACACCA 
GAAGAGTTTT GGAAACGACG GAAAATTGTG GATGGTAGAA TGATGACGTT TGTTGACGAG 
GTTCAGAAGC ATTTCCTAGG TGTTGCGGCG AGTCTTCTGA TGCCGTCTGG ACAGTTGGGC 
CCGAAAGCGG CAGAACTTGC AATTAAAATC CACAAGTTGT CGAAAGGAGG ACTCCTTCTG 
GGAGAGGCCA AGGAGATGGT ATATCAGTCA AAGTTGATGG ATGCGAAATC CTGGGAAGCG 
CTGATTCTTC GATTCTGTGA GATGAGAACT ACCGATGAGA AGTTCAAATC GTTCCTGCCG 
CTGATGCACA GGAATTCGGT TGAAGTAATG AATCAGGATG ATTCGATTGT TACGGAAAAG 
AAGTACACGT ACCTTGTCAT CTGTCCCCAT CTTTCTCAAT TCTGTTGGGA GCGATTGCCG 
ATTTTCGATG AGTATCCATA TGTGGGACGT CAAGTATCGA TTCACTCGAC TTTTAGTCAG 
TTGGAAGCAA TGAAAAGTCA GGAGAAACAG ATTCCTCTAC AAATCGACGT ACAAAACGCC 
TACTACATTC TCGATCCTGA CAACAATTTG GGCGAAACAC AAAAACGAAT GGTCGAATAT 
ATCAATAAGT TCAATTGGGA AGGAACTGTT GGCTCGGCGC CAAAATCGAA TGAGATTAGC 
GCCGCGTTGT CTCAACGCGA TGCGTTTTTC TTTATTGGAC ATGGCTCTGG ATCTTCGGTT 
ATGCCACGAT CTGTGCTCAA GCAGTCGACG TGTAATGCGA TCAGTTTGCT GATGGGTTGT 
GGAAGTGTCC GAACAATTCC ACAAGCTCTC GGTTTTGATG GAAAAACGGC GATTCTGGAC 
TACGCGATGG CAAAGTGTCC ACTGATCGTT GGATGCTTAT GGACTGTCAC TGATGGTGAA 
ATCGATCGTT TCTTGATTCG AATGATCGAT GATTGTTTCG AAGATTCCAA ATCGCTCACC 
GGAATCGACA AGCTTCGTCA GCTATCGGAG GCTATGCACG AGGCCAGATC AAAAGCCAGA 
CTGAAATATT TGACCGGTGC CGCTGTGGTG ATGTATGGAT TGCCAGTTGT TGCAAAGCAA 
ACGACGCCAT TCGTGGAAAA GGATCAGAGA AACTTGCCGC AGACTCCGAA GACTTCAGCC 
CGAACTTCGA TGCGAATGGA AACTGTGCCG AAGACACCGA AACAAGAATT TGTTACATCG 
AAGTCTGTTC CAATGACTCC AATTTTCTCA AATAATGAGA ACAAGTCTCC AAGCCGAGCA 
AGGATGCCAA GCAGAGTTCT GAAGACACCT CGTCAGGTGA AAACGTTCCA AGAGGAAGAT 
GATGAAGCTC CGAAACGGTC GACAACGCGG CAGCTGAAAC CGCTGGTTGC ACCACCTATT 
CCAGCAACAC CGACGACTAG AACAACTCGC TCAAGTGCCC GAACTCCATC AAGATCCCGA 
AATTTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sep-1 ( NM_058759.4 ) cDNA ORF clone, Caenorhabditis elegans -> NP_491160.1 Caenorhabditis elegans Separin homolog sep-1 (sep-1), mRNA.

Price & Availability↑

CloneID	OCa337089
Clone ID Related Accession (Same CDS sequence)	NM_058759.3 , NM_058759.4
Accession Version	NM_058759.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3789bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Separin homolog sep-1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003279). On Sep 4, 2019 this sequence version replaced NM_058759.3.

ATGAAGATCA CAAACAAGTC AGTTGACAAG CAGCACATCG AAAAACTAGA TGAGCTACGC 
AAAAATGTGA GCTGCACGGT GATCGGGTTT GCCGAACAAA CTGCCGAGTT GCAACAGGAG 
ATTTCCGAGC TTTTTATCGC CGAATTCGGC GTCAATGGAC CGATTGACAT GAACTCGCTG 
AGCAAATTGG CTCGAATCAC CTCATACTAC GCCTCGTCCG AATACTTTCA AGGACTTGCG 
AAGTATCAAC GAACCGCATG CAAAATGTTC ATCACGTGGC AAACTCTACG AAAGGAGGCG 
ATGGAGTGTC GGAGCAAAGA TCGAGAGATT TTCGCGAGTA TTCCCGCGAA ACTCTGCTTT 
TTCTACTTTT ACAACGGAGA ACTGTGCAGA GCAGTCGTGT GCCTGCTCGA CTACATCGAC 
CTATCAGACG ACACTCTGGC CAAGGAAGCT GCTCTTCGAT GGTTGATGTT TCTCGGCGAG 
ACCGAGCTAA TTGAGAAGAA GCTGAAGACG TGGAAAATGG ATAAAAGCTC GAAAGACATG 
TTCTCGGCTA CCGAATTCGC GATGAACTAC CTGAAAAAGT CAGAGTATCG CGTGGAAATG 
CTCGAAAAGC TGATGAAGCT TCGCGATAAA GTCAAATCGG ATCCTACTCG CTCATTTTCG 
AGATACGAGT TGGCTTCTTA TGTCTCTTGG TTGTGCTCAA CGTTGAGCAA TGTTCCCGTT 
GGATCCGCAC TCAGGGAGTG TGAGTTCCCG GATCGTGTGT CCCATATTCA AGAAGCAGCA 
TTAAAAAGTG ATTCACTTGT GCGTAATCGA ATTCCTGGCC TCGCCAGCTC TCAATTCGAC 
AACTCGGTTA ATGCATCAAT TTGGCCATTT CTAGACGGGC ATCAAGAAGA TAGCAATTAT 
TATGTGCACA TTGGCAGTAC AATCGCTTGG CACTTTGAGA TGCGTCGGGA GTGTGCTCTC 
GTCAACGTCA CCACCGCCCA AACTCGTGAT TCGATGTCGG CAATGATTCT GAATCTTCGT 
GTCGCATTAA AATCGGCTTC ATTCTTTCGA GTTCTTCAGA CAACCAACAC ATTAGCCTAC 
TATTCGAGTA TCATCGAAGA AGCCGGAAGT GAGAAGAACG CGAAATTAAT GAGAGTATCG 
TGTGTTAATT TGCTGTCGTC TAATCCGATA ATTGTTCGGT GTAGCACTCC GAAGGAGACG 
GGAGCCACTT CGAGAGCTCA TACACCGATG GCAGGGAGTA GTGTATCGGA GAAACAGAAC 
ACGATGAGAC CTGATCTGGC TGATCTGTTG GGAGATCTCG AATTACTCGA TGAACAATCA 
TTCCATCCGA TCACCCGATC CTGCGTCTGC AATGTCTGCA CGATTTACCC GCTGCATAGC 
TCATTCGCCG CCGAATACAT GATGTCCTAC GCAATTCACT CGGATTTCTC GCAACTCTCC 
ATCAAACATT TCAACGACGA GTTCGCGAGA ATCAGAGAGC GTGGAATGTC TAGTCAGGTT 
CTGATGCATC GGGACTCGAG TGTGAGGCCG AGACCGAATA TCATTCAAAA CGAGATTTTC 
GGCATGTGTG TGATCCGGTG GCTGACGAAA AAGTTGGATT CGAAAGAAAG TGCTGACGAG 
GATACGATGG AGATTTTCAA CAACGCGTTG AAGATCGTGA GATATCTTCA ACAACGAACA 
ACCGATATGA TTCTCGCAGT GACACAACTT GGACGACAAC TCGAGTTTCC CATGGAATGC 
AACTATAGCT GGATGCGGCC AACGATCCGG AAACCACGTG TCAAGGCAAC GATCGATTGT 
GCCGTCGACA TTCTTCGCGC GGTTTCTCCG TTCGGACGTC GTCCGAAAGT TGAGAAACTG 
GAGAAGAATC TTCAGCCTTT CGATAAGGAA CGCTTCGAGA AGGTTCGGCT TGCCATGCGG 
AACGAGATGA ACCACTACGG CCATATTCTA TACAGAGAAT GGCGTTGCCG CTTGTTCGCC 
TACGTCGGAA GAACATCACG TGATCCTTGG GAGGCTGCGT ACGCATGGGC CGAGTCAACA 
CAGATCGGAG CTCGTAACGC TGTACAGAGC CGACTTGAGA AGTGCAAGAG AGGATTGGTG 
ACAATGTCGG GACATGATCG CTTCAAAACG TGTGTACAGT CGATGCCAGA CGAGATGACG 
CTTGTACAGA TTGCGATGGC CGATGATAAG ACGATTTATT TGGTGAAACT GCACGCGGAT 
CGTGATCCGA TCATCATGCC ACTGGCACAC TACTCTCAAG CCGTCGAATT GATGGATAAA 
TTCACGTTTT TGTTGGATGA AGACGAGATG ATAGCAAAGT ATCCCGGAGA CATTACACCA 
GAAGAGTTTT GGAAACGACG GAAAATTGTG GATGGTAGAA TGATGACGTT TGTTGACGAG 
GTTCAGAAGC ATTTCCTAGG TGTTGCGGCG AGTCTTCTGA TGCCGTCTGG ACAGTTGGGC 
CCGAAAGCGG CAGAACTTGC AATTAAAATC CACAAGTTGT CGAAAGGAGG ACTCCTTCTG 
GGAGAGGCCA AGGAGATGGT ATATCAGTCA AAGTTGATGG ATGCGAAATC CTGGGAAGCG 
CTGATTCTTC GATTCTGTGA GATGAGAACT ACCGATGAGA AGTTCAAATC GTTCCTGCCG 
CTGATGCACA GGAATTCGGT TGAAGTAATG AATCAGGATG ATTCGATTGT TACGGAAAAG 
AAGTACACGT ACCTTGTCAT CTGTCCCCAT CTTTCTCAAT TCTGTTGGGA GCGATTGCCG 
ATTTTCGATG AGTATCCATA TGTGGGACGT CAAGTATCGA TTCACTCGAC TTTTAGTCAG 
TTGGAAGCAA TGAAAAGTCA GGAGAAACAG ATTCCTCTAC AAATCGACGT ACAAAACGCC 
TACTACATTC TCGATCCTGA CAACAATTTG GGCGAAACAC AAAAACGAAT GGTCGAATAT 
ATCAATAAGT TCAATTGGGA AGGAACTGTT GGCTCGGCGC CAAAATCGAA TGAGATTAGC 
GCCGCGTTGT CTCAACGCGA TGCGTTTTTC TTTATTGGAC ATGGCTCTGG ATCTTCGGTT 
ATGCCACGAT CTGTGCTCAA GCAGTCGACG TGTAATGCGA TCAGTTTGCT GATGGGTTGT 
GGAAGTGTCC GAACAATTCC ACAAGCTCTC GGTTTTGATG GAAAAACGGC GATTCTGGAC 
TACGCGATGG CAAAGTGTCC ACTGATCGTT GGATGCTTAT GGACTGTCAC TGATGGTGAA 
ATCGATCGTT TCTTGATTCG AATGATCGAT GATTGTTTCG AAGATTCCAA ATCGCTCACC 
GGAATCGACA AGCTTCGTCA GCTATCGGAG GCTATGCACG AGGCCAGATC AAAAGCCAGA 
CTGAAATATT TGACCGGTGC CGCTGTGGTG ATGTATGGAT TGCCAGTTGT TGCAAAGCAA 
ACGACGCCAT TCGTGGAAAA GGATCAGAGA AACTTGCCGC AGACTCCGAA GACTTCAGCC 
CGAACTTCGA TGCGAATGGA AACTGTGCCG AAGACACCGA AACAAGAATT TGTTACATCG 
AAGTCTGTTC CAATGACTCC AATTTTCTCA AATAATGAGA ACAAGTCTCC AAGCCGAGCA 
AGGATGCCAA GCAGAGTTCT GAAGACACCT CGTCAGGTGA AAACGTTCCA AGAGGAAGAT 
GATGAAGCTC CGAAACGGTC GACAACGCGG CAGCTGAAAC CGCTGGTTGC ACCACCTATT 
CCAGCAACAC CGACGACTAG AACAACTCGC TCAAGTGCCC GAACTCCATC AAGATCCCGA 
AATTTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_491160.1
CDS	11..3799
Translation	MKITNKSVDKQHIEKLDELRKNVSCTVIGFAEQTAELQQEISELFIAEFGVNGPIDMNSLSKLARITSYYASSEYFQGLAKYQRTACKMFITWQTLRKEAMECRSKDREIFASIPAKLCFFYFYNGELCRAVVCLLDYIDLSDDTLAKEAALRWLMFLGETELIEKKLKTWKMDKSSKDMFSATEFAMNYLKKSEYRVEMLEKLMKLRDKVKSDPTRSFSRYELASYVSWLCSTLSNVPVGSALRECEFPDRVSHIQEAALKSDSLVRNRIPGLASSQFDNSVNASIWPFLDGHQEDSNYYVHIGSTIAWHFEMRRECALVNVTTAQTRDSMSAMILNLRVALKSASFFRVLQTTNTLAYYSSIIEEAGSEKNAKLMRVSCVNLLSSNPIIVRCSTPKETGATSRAHTPMAGSSVSEKQNTMRPDLADLLGDLELLDEQSFHPITRSCVCNVCTIYPLHSSFAAEYMMSYAIHSDFSQLSIKHFNDEFARIRERGMSSQVLMHRDSSVRPRPNIIQNEIFGMCVIRWLTKKLDSKESADEDTMEIFNNALKIVRYLQQRTTDMILAVTQLGRQLEFPMECNYSWMRPTIRKPRVKATIDCAVDILRAVSPFGRRPKVEKLEKNLQPFDKERFEKVRLAMRNEMNHYGHILYREWRCRLFAYVGRTSRDPWEAAYAWAESTQIGARNAVQSRLEKCKRGLVTMSGHDRFKTCVQSMPDEMTLVQIAMADDKTIYLVKLHADRDPIIMPLAHYSQAVELMDKFTFLLDEDEMIAKYPGDITPEEFWKRRKIVDGRMMTFVDEVQKHFLGVAASLLMPSGQLGPKAAELAIKIHKLSKGGLLLGEAKEMVYQSKLMDAKSWEALILRFCEMRTTDEKFKSFLPLMHRNSVEVMNQDDSIVTEKKYTYLVICPHLSQFCWERLPIFDEYPYVGRQVSIHSTFSQLEAMKSQEKQIPLQIDVQNAYYILDPDNNLGETQKRMVEYINKFNWEGTVGSAPKSNEISAALSQRDAFFFIGHGSGSSVMPRSVLKQSTCNAISLLMGCGSVRTIPQALGFDGKTAILDYAMAKCPLIVGCLWTVTDGEIDRFLIRMIDDCFEDSKSLTGIDKLRQLSEAMHEARSKARLKYLTGAAVVMYGLPVVAKQTTPFVEKDQRNLPQTPKTSARTSMRMETVPKTPKQEFVTSKSVPMTPIFSNNENKSPSRARMPSRVLKTPRQVKTFQEEDDEAPKRSTTRQLKPLVAPPIPATPTTRTTRSSARTPSRSRNL

Target ORF information:

RefSeq Version	NM_058759.4
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Separin homolog sep-1 (sep-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_058759.4

ORF Insert Sequence:

ATGAAGATCA CAAACAAGTC AGTTGACAAG CAGCACATCG AAAAACTAGA TGAGCTACGC 
AAAAATGTGA GCTGCACGGT GATCGGGTTT GCCGAACAAA CTGCCGAGTT GCAACAGGAG 
ATTTCCGAGC TTTTTATCGC CGAATTCGGC GTCAATGGAC CGATTGACAT GAACTCGCTG 
AGCAAATTGG CTCGAATCAC CTCATACTAC GCCTCGTCCG AATACTTTCA AGGACTTGCG 
AAGTATCAAC GAACCGCATG CAAAATGTTC ATCACGTGGC AAACTCTACG AAAGGAGGCG 
ATGGAGTGTC GGAGCAAAGA TCGAGAGATT TTCGCGAGTA TTCCCGCGAA ACTCTGCTTT 
TTCTACTTTT ACAACGGAGA ACTGTGCAGA GCAGTCGTGT GCCTGCTCGA CTACATCGAC 
CTATCAGACG ACACTCTGGC CAAGGAAGCT GCTCTTCGAT GGTTGATGTT TCTCGGCGAG 
ACCGAGCTAA TTGAGAAGAA GCTGAAGACG TGGAAAATGG ATAAAAGCTC GAAAGACATG 
TTCTCGGCTA CCGAATTCGC GATGAACTAC CTGAAAAAGT CAGAGTATCG CGTGGAAATG 
CTCGAAAAGC TGATGAAGCT TCGCGATAAA GTCAAATCGG ATCCTACTCG CTCATTTTCG 
AGATACGAGT TGGCTTCTTA TGTCTCTTGG TTGTGCTCAA CGTTGAGCAA TGTTCCCGTT 
GGATCCGCAC TCAGGGAGTG TGAGTTCCCG GATCGTGTGT CCCATATTCA AGAAGCAGCA 
TTAAAAAGTG ATTCACTTGT GCGTAATCGA ATTCCTGGCC TCGCCAGCTC TCAATTCGAC 
AACTCGGTTA ATGCATCAAT TTGGCCATTT CTAGACGGGC ATCAAGAAGA TAGCAATTAT 
TATGTGCACA TTGGCAGTAC AATCGCTTGG CACTTTGAGA TGCGTCGGGA GTGTGCTCTC 
GTCAACGTCA CCACCGCCCA AACTCGTGAT TCGATGTCGG CAATGATTCT GAATCTTCGT 
GTCGCATTAA AATCGGCTTC ATTCTTTCGA GTTCTTCAGA CAACCAACAC ATTAGCCTAC 
TATTCGAGTA TCATCGAAGA AGCCGGAAGT GAGAAGAACG CGAAATTAAT GAGAGTATCG 
TGTGTTAATT TGCTGTCGTC TAATCCGATA ATTGTTCGGT GTAGCACTCC GAAGGAGACG 
GGAGCCACTT CGAGAGCTCA TACACCGATG GCAGGGAGTA GTGTATCGGA GAAACAGAAC 
ACGATGAGAC CTGATCTGGC TGATCTGTTG GGAGATCTCG AATTACTCGA TGAACAATCA 
TTCCATCCGA TCACCCGATC CTGCGTCTGC AATGTCTGCA CGATTTACCC GCTGCATAGC 
TCATTCGCCG CCGAATACAT GATGTCCTAC GCAATTCACT CGGATTTCTC GCAACTCTCC 
ATCAAACATT TCAACGACGA GTTCGCGAGA ATCAGAGAGC GTGGAATGTC TAGTCAGGTT 
CTGATGCATC GGGACTCGAG TGTGAGGCCG AGACCGAATA TCATTCAAAA CGAGATTTTC 
GGCATGTGTG TGATCCGGTG GCTGACGAAA AAGTTGGATT CGAAAGAAAG TGCTGACGAG 
GATACGATGG AGATTTTCAA CAACGCGTTG AAGATCGTGA GATATCTTCA ACAACGAACA 
ACCGATATGA TTCTCGCAGT GACACAACTT GGACGACAAC TCGAGTTTCC CATGGAATGC 
AACTATAGCT GGATGCGGCC AACGATCCGG AAACCACGTG TCAAGGCAAC GATCGATTGT 
GCCGTCGACA TTCTTCGCGC GGTTTCTCCG TTCGGACGTC GTCCGAAAGT TGAGAAACTG 
GAGAAGAATC TTCAGCCTTT CGATAAGGAA CGCTTCGAGA AGGTTCGGCT TGCCATGCGG 
AACGAGATGA ACCACTACGG CCATATTCTA TACAGAGAAT GGCGTTGCCG CTTGTTCGCC 
TACGTCGGAA GAACATCACG TGATCCTTGG GAGGCTGCGT ACGCATGGGC CGAGTCAACA 
CAGATCGGAG CTCGTAACGC TGTACAGAGC CGACTTGAGA AGTGCAAGAG AGGATTGGTG 
ACAATGTCGG GACATGATCG CTTCAAAACG TGTGTACAGT CGATGCCAGA CGAGATGACG 
CTTGTACAGA TTGCGATGGC CGATGATAAG ACGATTTATT TGGTGAAACT GCACGCGGAT 
CGTGATCCGA TCATCATGCC ACTGGCACAC TACTCTCAAG CCGTCGAATT GATGGATAAA 
TTCACGTTTT TGTTGGATGA AGACGAGATG ATAGCAAAGT ATCCCGGAGA CATTACACCA 
GAAGAGTTTT GGAAACGACG GAAAATTGTG GATGGTAGAA TGATGACGTT TGTTGACGAG 
GTTCAGAAGC ATTTCCTAGG TGTTGCGGCG AGTCTTCTGA TGCCGTCTGG ACAGTTGGGC 
CCGAAAGCGG CAGAACTTGC AATTAAAATC CACAAGTTGT CGAAAGGAGG ACTCCTTCTG 
GGAGAGGCCA AGGAGATGGT ATATCAGTCA AAGTTGATGG ATGCGAAATC CTGGGAAGCG 
CTGATTCTTC GATTCTGTGA GATGAGAACT ACCGATGAGA AGTTCAAATC GTTCCTGCCG 
CTGATGCACA GGAATTCGGT TGAAGTAATG AATCAGGATG ATTCGATTGT TACGGAAAAG 
AAGTACACGT ACCTTGTCAT CTGTCCCCAT CTTTCTCAAT TCTGTTGGGA GCGATTGCCG 
ATTTTCGATG AGTATCCATA TGTGGGACGT CAAGTATCGA TTCACTCGAC TTTTAGTCAG 
TTGGAAGCAA TGAAAAGTCA GGAGAAACAG ATTCCTCTAC AAATCGACGT ACAAAACGCC 
TACTACATTC TCGATCCTGA CAACAATTTG GGCGAAACAC AAAAACGAAT GGTCGAATAT 
ATCAATAAGT TCAATTGGGA AGGAACTGTT GGCTCGGCGC CAAAATCGAA TGAGATTAGC 
GCCGCGTTGT CTCAACGCGA TGCGTTTTTC TTTATTGGAC ATGGCTCTGG ATCTTCGGTT 
ATGCCACGAT CTGTGCTCAA GCAGTCGACG TGTAATGCGA TCAGTTTGCT GATGGGTTGT 
GGAAGTGTCC GAACAATTCC ACAAGCTCTC GGTTTTGATG GAAAAACGGC GATTCTGGAC 
TACGCGATGG CAAAGTGTCC ACTGATCGTT GGATGCTTAT GGACTGTCAC TGATGGTGAA 
ATCGATCGTT TCTTGATTCG AATGATCGAT GATTGTTTCG AAGATTCCAA ATCGCTCACC 
GGAATCGACA AGCTTCGTCA GCTATCGGAG GCTATGCACG AGGCCAGATC AAAAGCCAGA 
CTGAAATATT TGACCGGTGC CGCTGTGGTG ATGTATGGAT TGCCAGTTGT TGCAAAGCAA 
ACGACGCCAT TCGTGGAAAA GGATCAGAGA AACTTGCCGC AGACTCCGAA GACTTCAGCC 
CGAACTTCGA TGCGAATGGA AACTGTGCCG AAGACACCGA AACAAGAATT TGTTACATCG 
AAGTCTGTTC CAATGACTCC AATTTTCTCA AATAATGAGA ACAAGTCTCC AAGCCGAGCA 
AGGATGCCAA GCAGAGTTCT GAAGACACCT CGTCAGGTGA AAACGTTCCA AGAGGAAGAT 
GATGAAGCTC CGAAACGGTC GACAACGCGG CAGCTGAAAC CGCTGGTTGC ACCACCTATT 
CCAGCAACAC CGACGACTAG AACAACTCGC TCAAGTGCCC GAACTCCATC AAGATCCCGA 
AATTTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.
Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

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sep-1 cDNA ORF clone, Caenorhabditis elegans

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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