PCM1 gene cDNA ORF clone, Bubalus bubalis(water buffalo)

mRNA	Protein	Name
XM_006080136.1	XP_006080198.1	pericentriolar material 1 protein isoform X3
XM_006080140.1	XP_006080202.1	pericentriolar material 1 protein isoform X7
XM_006080142.1	XP_006080204.1	pericentriolar material 1 protein isoform X9
XM_025279471.1	XP_025135256.1	pericentriolar material 1 protein isoform X10
XM_025279498.1	XP_025135283.1	pericentriolar material 1 protein isoform X16
XM_025279520.1	XP_025135305.1	pericentriolar material 1 protein isoform X20
XM_025279531.1	XP_025135316.1	pericentriolar material 1 protein isoform X22
XM_006080134.1	XP_006080196.1	pericentriolar material 1 protein isoform X1
XM_025279414.1	XP_025135199.1	pericentriolar material 1 protein isoform X2
XM_025279423.1	XP_025135208.1	pericentriolar material 1 protein isoform X3
XM_025279460.1	XP_025135245.1	pericentriolar material 1 protein isoform X8
XM_025279526.1	XP_025135311.1	pericentriolar material 1 protein isoform X21
XM_006080135.1	XP_006080197.1	pericentriolar material 1 protein isoform X2
XM_006080139.1	XP_006080201.1	pericentriolar material 1 protein isoform X6
XM_025279429.1	XP_025135214.1	pericentriolar material 1 protein isoform X4
XM_025279491.1	XP_025135276.1	pericentriolar material 1 protein isoform X15
XM_025279539.1	XP_025135324.1	pericentriolar material 1 protein isoform X23
XM_006080137.1	XP_006080199.1	pericentriolar material 1 protein isoform X4
XM_006080138.1	XP_006080200.1	pericentriolar material 1 protein isoform X5
XM_025279405.1	XP_025135190.1	pericentriolar material 1 protein isoform X1
XM_025279407.1	XP_025135192.1	pericentriolar material 1 protein isoform X1
XM_025279446.1	XP_025135231.1	pericentriolar material 1 protein isoform X6
XM_025279466.1	XP_025135251.1	pericentriolar material 1 protein isoform X9
XM_025279487.1	XP_025135272.1	pericentriolar material 1 protein isoform X14
XM_025279509.1	XP_025135294.1	pericentriolar material 1 protein isoform X18
XM_025279513.1	XP_025135298.1	pericentriolar material 1 protein isoform X19
XM_025279544.1	XP_025135329.1	pericentriolar material 1 protein isoform X24
XM_006080141.1	XP_006080203.1	pericentriolar material 1 protein isoform X8
XM_025279440.1	XP_025135225.1	pericentriolar material 1 protein isoform X5
XM_025279455.1	XP_025135240.1	pericentriolar material 1 protein isoform X7
XM_025279478.1	XP_025135263.1	pericentriolar material 1 protein isoform X11
XM_025279480.1	XP_025135265.1	pericentriolar material 1 protein isoform X12
XM_025279484.1	XP_025135269.1	pericentriolar material 1 protein isoform X13
XM_025279504.1	XP_025135289.1	pericentriolar material 1 protein isoform X17

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBu33069	XM_006080142.1 Latest version!	Bubalus bubalis pericentriolar material 1 (PCM1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OBu42290	XM_025279405.1 Latest version!	Bubalus bubalis pericentriolar material 1 (PCM1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBu42290	XM_025279407.1 Latest version!	Bubalus bubalis pericentriolar material 1 (PCM1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBu42291	XM_025279414.1 Latest version!	Bubalus bubalis pericentriolar material 1 (PCM1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBu42292	XM_025279423.1 Latest version!	Bubalus bubalis pericentriolar material 1 (PCM1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBu42293	XM_025279429.1 Latest version!	Bubalus bubalis pericentriolar material 1 (PCM1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBu42294	XM_025279440.1 Latest version!	Bubalus bubalis pericentriolar material 1 (PCM1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBu42295	XM_025279446.1 Latest version!	Bubalus bubalis pericentriolar material 1 (PCM1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBu42296	XM_025279455.1 Latest version!	Bubalus bubalis pericentriolar material 1 (PCM1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBu42297	XM_025279460.1 Latest version!	Bubalus bubalis pericentriolar material 1 (PCM1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBu42298	XM_025279466.1 Latest version!	Bubalus bubalis pericentriolar material 1 (PCM1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBu42299	XM_025279471.1 Latest version!	Bubalus bubalis pericentriolar material 1 (PCM1), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBu42300	XM_025279478.1 Latest version!	Bubalus bubalis pericentriolar material 1 (PCM1), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBu42301	XM_025279480.1 Latest version!	Bubalus bubalis pericentriolar material 1 (PCM1), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBu42302	XM_025279484.1 Latest version!	Bubalus bubalis pericentriolar material 1 (PCM1), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBu42303	XM_025279487.1 Latest version!	Bubalus bubalis pericentriolar material 1 (PCM1), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBu42304	XM_025279491.1 Latest version!	Bubalus bubalis pericentriolar material 1 (PCM1), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBu42305	XM_025279498.1 Latest version!	Bubalus bubalis pericentriolar material 1 (PCM1), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBu42306	XM_025279504.1 Latest version!	Bubalus bubalis pericentriolar material 1 (PCM1), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBu42307	XM_025279509.1 Latest version!	Bubalus bubalis pericentriolar material 1 (PCM1), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBu42308	XM_025279513.1 Latest version!	Bubalus bubalis pericentriolar material 1 (PCM1), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBu42309	XM_025279520.1 Latest version!	Bubalus bubalis pericentriolar material 1 (PCM1), transcript variant X21, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBu42310	XM_025279526.1 Latest version!	Bubalus bubalis pericentriolar material 1 (PCM1), transcript variant X22, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBu42311	XM_025279531.1 Latest version!	Bubalus bubalis pericentriolar material 1 (PCM1), transcript variant X23, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBu42312	XM_025279539.1 Latest version!	Bubalus bubalis pericentriolar material 1 (PCM1), transcript variant X24, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBu42313	XM_025279544.1 Latest version!	Bubalus bubalis pericentriolar material 1 (PCM1), transcript variant X25, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBu33062	XM_006080134.1 Latest version!	Bubalus bubalis pericentriolar material 1 (PCM1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OBu33062	XM_006080135.1 Latest version!	Bubalus bubalis pericentriolar material 1 (PCM1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OBu33063	XM_006080136.1 Latest version!	Bubalus bubalis pericentriolar material 1 (PCM1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OBu33064	XM_006080137.1 Latest version!	Bubalus bubalis pericentriolar material 1 (PCM1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OBu33065	XM_006080138.1 Latest version!	Bubalus bubalis pericentriolar material 1 (PCM1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OBu33066	XM_006080139.1 Latest version!	Bubalus bubalis pericentriolar material 1 (PCM1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OBu33067	XM_006080140.1 Latest version!	Bubalus bubalis pericentriolar material 1 (PCM1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OBu33068	XM_006080141.1 Latest version!	Bubalus bubalis pericentriolar material 1 (PCM1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00

PCM1 ( XM_006080142.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006080204.1 Bubalus bubalis pericentriolar material 1 (PCM1), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OBu33069
Clone ID Related Accession (Same CDS sequence)	XM_006080142.1
Accession Version	XM_006080142.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6270bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	pericentriolar material 1 protein isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785878.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG GAGGAGGTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
AGCAATGAGA GTGTTGATGA CCGACTCAAC AATATGGATT GGGGCAGCCA GCAGAAGAAG 
GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA 
ACTAACGATA TTTCTCCGGA GTCATCACCG GGAGTTGGAA GACGAAGAAC AAAGACTTCA 
CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TCCCCGAGCA GGCAGAACTA 
GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGATTAATAC TAACAAGAGC 
AAAGATGCCA CTGTAAGTCC TCAAAAGAGA GAAATGATTG GATCAGCACA GTGTAAAGAG 
TTGTTTGCTT CTGCTTTAAG TAATGACCTT TTGCAAAACT GTCAAGTCTC TGAAGAAGAT 
GGAAGAGGAG AACCTGCAAT GGAGAGCAGC CAGATTGTAA GCAGGCTTGT TCAGATTCGT 
GAGTATATTA CTAAAGCTAG TTCCATGCGG GAAGACCTTG TAGAGAAAAA TGAAAGATCT 
GCTAATGTTG AGCGCCTCAC TCATCTAATA GATCACCTTA AAGAACAAGA AAAGTCATAT 
ATGAAATTTC TCCAGAAGAT TCTTGCCAGA GATCCTCAGC AGGAGCCTAT GGAAGAGATA 
GAAAATTTGA AGAAACAACA TGATTTATTA AAAAGGATGT TACAGCAGCA GGAGCAGCTG 
AGAGCTCTAC AAGGACGACA AGCTGCTCTG CTTGCCCTGC AGCATAAAGC TGAGCAGGCT 
ATAGCTGTGA TGGATGATTC TGAAAAGGTT GCGGAGACAA CTCCTGGCCA TCATACTGTA 
CCAGGCAGAC AGCCGGCTCA TTCACCTTTG CATCAGAGAG TACCCATAAG AGAAACTACA 
GGTAGCCTAT CTGGAGTCAG TATCACCTCT GAACTAAACG AAGAATTGAA TGATTTAATT 
CAGCATTTTC ATAATCAGCT TCGTGATTCT CAGCCTCCAG CTGTTCCAGA CAATAGAAGA 
CAAGCAGAAA GTCTTTCATT AACTAGGGAG ATTTCTCAGA GCAGAAATCC TTCAGTTTCA 
GAGCATTTAC CTGATGAGAA AGTCCAACTT TTTAGCAAGA TGAGAGTGTT ACAAGAAAAG 
AAACAAAAAA TGGACAAACT TCTTGGAGAA CTTCATACAC TTCGAGATCA GCATTTGAAC 
AATTCATCAT TTGTGCCTTC TTCAGCCTCT CCACAAAGGA GTGTGGATCA GAGAAGTACA 
ACCACAGCTC CTTCTGCTCC TGTAGTCTTA ACACCAGTGG TCAATGGAGA ATCCAATAAC 
CTCACGCCAT CTGTTCCTTA TCCTGCTGCT TCTCTAGTTT CTCAGAATCA GAATGAAAAT 
GAAGGCCACC TAAATCCAAC TGAAAAACTC CAGAAGTTAA ATGAAGTTCG AAAGAGGTTG 
AATGAACTAA GAGAATTAGT TCATTATTAT GAGCAAACAT CAGATATGAT GACAGATGCT 
GTGAATGAAA ACACAAAAGA AGAAGAAACT GAAGAATCAG AATATGATTC GGAGCATGAA 
AACTCGGAAC CTGTTACTAA CATTCGAAAT CCACAAGTGG CTGCCACCTG GAACGAAGTA 
AATAGTAATT CTAATGCACA GTGTGTTTCC AACAATAGAG ATGGGCGAGC AGTTAATTCT 
AACTGTGAAA TTAACAACAG GTCTGCTGCC AACATAAGGG CTCTAAACAT GCCTCCTCTT 
TCAGCAGATT GTCGATACAA TAGAGAAGGA GAACAGGAGA TGCCTGTTGC ACAAGGTCAT 
GAGGAGGAGG AGGAGGAGGA GGAGGAAGCA GAAGATGAGG CCCTCAGTGG AGCTTCACTG 
TCTAGTCACA GCAGCAGCCT GATCATTGAG ACTCCTGAAG ATGCTGAATT TGAACATAAG 
ATCCAGAAGC TTATGGCTGC AAAACTGAAA CTTAGACACT TACAGAACCT TGTAGCCATG 
GTGCAGGACG ATGATGCAGC CAATCAAGGA GTTACTTCTG TGGAGGATTT CTTCCCAGCA 
GAAGACACCA AACAAAATGC AAATAACACT AGAGGAAATG CCAATAAAAC ACAGAAAGAT 
GCTGGAGTAA ATGAAAAGGC AAGGGAGAAA TTTTATGAGG CTAAACTACA GCAGCAACAG 
AGAGAACTCA AACAATTGCA GGAAGAAAGA AAGAAACTGA TTGAGATTCA AGAGAAAATT 
CAAGAATTGC AAAAGGCATG TCCTAACCTA CAGCTGTCAG CTGCTAGTGT GGGTAATTGT 
CCCACAAAAA CACATATGCC AGCTGTTACT TCATCCCCAA CCGTTAATGA AAATGAAGCC 
AGTACAAACA AATCTGGTTT TGAGCCTCAT GATTCTTCAG TGGTAGATAA TGAGGTATGG 
TCAGATATGC GAAGACATGA AATGTTAAGG GAGGAGCTAC GACAGAGAAG AAAGCAGCTG 
GAAGCTCTGA TGGCTGAACA TCAGAGGAGG CAAGGTCTGG CTGAAACTGC ATCTCCAGTG 
GCTGTTTCAC TGAGAAGTGA TGGATCTGAG AACCTGTGTA CACCTCAGCA AAGCAGAACA 
GAAAAAACAA TGGCAACTTG GGGAGGTTCT ACCCAGTGTG CCCTAGATGA AGAAGGAGAT 
GAAGATGGTT ACCTTTCTGA AGGAATTATT CGGACAGACG AAGAGGAGGA AGAGGAGGAG 
CAAGATGCCA GTTCCAATGA TAACTTTCAT ATGTATCCTC CAAACAGTGC AAATCATAAT 
TCTTACAATG TAAAGGAAAC TAAAAATAGG TGGAAGAACA ATCGCCCTTT TTCAGCAGAT 
GGAAATTATC GTCCTTTAGC CAAGACAAGA CAGCAGAATA TCAGCATGCA GCGTCAGGAA 
AACCTTCGCT GGGTGTCCGA ACTCTCTTAT GTAGAAGAGA AAGAACAATG GCAAGAACAA 
ATTAATCAGC TAAAGAAACA ACTTGATTTC AGTGTCAATA TTTGTCAGAC TTTAATGCAA 
GACCAGCAGA CTCTGTCTTG TCTGCTACAA ACTCTTCTCA CGAGTCCTTA TAGTGTTATG 
CCCAGCAGTG TTGCATCCCC GCAAGTGCAC CTTATAATGC ACCAGTTGAA TCAGTGCTAT 
ACCCAGCTAA CGTGGCAGCA GAACAATGTC CAGAGGTTGA AACAAATGCT CAGTGAACTC 
ATGCGCCAAC AAAATCAGCA TCCAGAAAAA CCTGCAAGCA AGGAAGGAGG CAGTGGTACA 
TCACACCCTC CTTCTCCCAG TTTATTTTGT CCTTTCAACT TTCCAACTCA GCCTGTAAAT 
CTGTTTAACC TACCGGGGTT TACTAATTTT TCGTCATTTG CACCAAGTAT GAATTTCAGC 
CCTTTATTTC CTTCTAATTT TGGAGATTTT TCTCAAAATA TTTCTACACC CACTGAACAG 
CAGCAACCGT TAGCCCAGAA TCCTTCAGGG AAAACGGAAT ACATGGCTTT TCCAAAACCT 
TTTGAAAGTA GTTCTTCTAT TGGAGCGGAA AAACAAAGGA ATCAAAAACA GCCTGAAGAG 
GAGGTGGAGA ACAGTAGGAC ACCATGGTTT TATGACCAAG AAGGTGAAGT AGAAAAGCCA 
TTTCTCAAGA CTGGATTTGC AGTGTCTGTA GAGAAAACAA CAAATAGTAA TCGCAAAAAT 
CAACTAGATA CCAGTAGGAG AAGACGCCAG TTTGATGAAG TGTCCTTAGA AAGCTTTAAC 
AGTATGCTTG ACCCAGTAGA TCCAACAACA GTAACTAAAA CATTCAAGTC AAGAAAAGCA 
TCTGCACAGG CCAGCCTGGC ATCTAAAGAT AAAACTCCCA AATCAAAGAG TAAGAAGAGG 
CATTCTACTC AGCTGAAAAG CAGAGTGAAA AATATCGGGT ATGAAAGTGC CAGTGTGTCT 
AGCACATGTG AACCTTGCAA AAGTAGAAAC AGACATTCAG CCCAAACTGA AGAGCCTGTT 
CAAGCAAAAG TACTCAGCAG AAAGAATCAT GAGCAGCTGG AAAAAGTCAT AAGATGTAGT 
AGGTCTACAG AAATATCTTC AGCACATGCT AGGAGAATAC TACAGCAGTC TAACAGAAAT 
GCATGCAATG AAGCGCCAGA AACCGGGAGT GATTTTTCCA TGTTCGAAGC TTTGCGGGAT 
ACCATTTATT CTGAAGTAGC TACGTTGATT TCTCAAAATG AATCTCGTCC ACATTTTCTT 
ATTGAACTCT TCCATGAGCT TCAGCTACTT AATACAGACT ATTTGAGACA GAGGGCCTTA 
TATGCATTGC AGGACATAGT ATCCAGGCAT ATTTCTGAGA ACCATGAAAA AGAAGGAGAA 
AATATAAAGT CCGTGAATTC TGGTACTTGG ATAGCATCCA ACTCAGAACT TACTCCCAGT 
GAAAGCCTTG CTACTACTGA TGATGAAACT TTTGAGAAGA ACTTTGAGAG AGAAACACAT 
AAAATAAGTG AGCAAAATGA TGCTGATAAT GCTAGTGTAT CTTCAACTTG TGAGCCTTTT 
GCAACAGATG TTCTGGGCGA CACTGTGATT CACTTAGATC AGGCATTAGC CAGAATGAGA 
GACTATACGC ATATGAAGAT GGAGGCTGAA AGTAGTCCAG ACGTCAGGTG CACCTGCAGG 
ATCATTGAGG ATGACGATGG TGCTGCTGCC ACAACCACGA TTAGTAATAA TTTAGAAGAA 
ACTCCTGTTA TTGAAAATCA TAGTTCACAG CAACCCGTGA GTGAAGTTGC TACCGTCCCG 
TGTCCTAGAA TTGATACTCA GCAACTGGAC CGGCAAATTA AAGCAATTAT GAAAGAAGTC 
ATTCCTTTTT TGAAGGAGCA CATGGATGAA GTATGTTCTT CTCAACTTCT AACCTCAGTA 
AGACGCATGG TTTTGGTCCT TACTCAGCAA AACAATGAGA GCAAAGAGTT TGTAAAGTTC 
TTTCATAAGC AGCTTGGAAG TATATTACAG GATTCACTCG CAAAATTTGC TGGCAGAAAA 
CTGAAGGATT GTGGAGAAGA TCTTCTTGTA GAGATATCTG AAGTGTTATT TAATGAATTG 
GCTTTCTTTA AGCTCATGCA AGATTTGGAT AATAATAGTG TAACTGTTAA ACAGAGATGC 
AAAAGGAAAA TAGAAGCTGC TGGAGTGATA CAGTCTTATG CAAAAGAGGC CAAAAGGATT 
CTTGAAGGAG ATCACAGTTC ACCTGCTGGA GAAATTGATG ATGAAGACAA AGACAAGGAT 
GAGACGGAAA CAGTTAAGCA GACTCAAACG TCTGAGGTTT ATGATGGTGA CGGCCCCAAA 
AATGTGAGAT CTGATGTTTC TGATCAAGAG GATGATGAAG AAAGTGAAGA ATGTCCAGTG 
TCTATTAATT TGTCCAAAGC TGAAACTCAG GCTTTGACTA ATTACGGAAG TGGAGAAGAT 
GAGAATGAAG ATGAAGAAAT AGAAGAATTT GAAGAGGGAC CTGTAGATGT CCAGACGTCA 
CTTCAGGCTA ACACTGAAAC TACAGAAGAA AATGAACACG ATGATCAGGT TCCACAGCAT 
GATTTTGAAA AATCATCAGA AAGCAAAAAT GTCCCATCAG AACAAGAACC CACTACTAGT 
AAAGGTGACC AGAATAGCAC TCCTGTGAAG CCCTGTTACC TCAGTATCGT GGAAAATGAG 
CAACCTTTAA ATAGCCCTGT CCAGAAGGAC ACTCTCACTA CCATCGACTC ATCCAGTCAG 
CCTAACCCTT TGCCTTTACC TTTAACTGAA ATTGAAACCT TGGTGCCTAG AGTCAAAGAA 
GTGAAATCTG CTCAGGAGAC TCCTGAAAGT TCTTTGGCTG GAAGTCCTGA TACTGAATCT 
CCAGTGTTAG TGAATGACTA TGAAGCAGAA TCTGGTAACA TAAGTCAAAA ATCTGATGAA 
GAAGACTTTG TGAAAGTTGA AGATTTACCA CTTAAACTGA CAATATATTC AGAGGCAGAT 
CTAAGGAAGA AAATGGCCGA AGAAGAACAG AAAAACCACT TATCTGGTGA AATATGTGCA 
ATGCAGACTG AAGAATTAGC TGGGAATTCT CAGACACTAA AAGAACCTGA GCAAGTATGT 
GCTCAGTCTG CCATCTTAAG ATGGAAACCC TTACCTGTGC CTTTTAAACT GTTGGTGGTG 
TCATCAGGGA ATTATTGTAG CAAAGTAAGT TTCTTTTCTA ATAACTTTAT ATTAAAATTG 
AAAGTTTTAA ATGGAAGAGA ACAATATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006080204.1
CDS	113..6382
Translation	MATGGGPFEEGVNDQDLPNWSNESVDDRLNNMDWGSQQKKANRSSEKNKRKFGVESDKRVTNDISPESSPGVGRRRTKTSLTFPHSRYMTQMSVPEQAELEKLKQRINFSDLDQINTNKSKDATVSPQKREMIGSAQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIREYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLQKILARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSEKVAETTPGHHTVPGRQPAHSPLHQRVPIRETTGSLSGVSITSELNEELNDLIQHFHNQLRDSQPPAVPDNRRQAESLSLTREISQSRNPSVSEHLPDEKVQLFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSFVPSSASPQRSVDQRSTTTAPSAPVVLTPVVNGESNNLTPSVPYPAASLVSQNQNENEGHLNPTEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENTKEEETEESEYDSEHENSEPVTNIRNPQVAATWNEVNSNSNAQCVSNNRDGRAVNSNCEINNRSAANIRALNMPPLSADCRYNREGEQEMPVAQGHEEEEEEEEEAEDEALSGASLSSHSSSLIIETPEDAEFEHKIQKLMAAKLKLRHLQNLVAMVQDDDAANQGVTSVEDFFPAEDTKQNANNTRGNANKTQKDAGVNEKAREKFYEAKLQQQQRELKQLQEERKKLIEIQEKIQELQKACPNLQLSAASVGNCPTKTHMPAVTSSPTVNENEASTNKSGFEPHDSSVVDNEVWSDMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETASPVAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGIIRTDEEEEEEEQDASSNDNFHMYPPNSANHNSYNVKETKNRWKNNRPFSADGNYRPLAKTRQQNISMQRQENLRWVSELSYVEEKEQWQEQINQLKKQLDFSVNICQTLMQDQQTLSCLLQTLLTSPYSVMPSSVASPQVHLIMHQLNQCYTQLTWQQNNVQRLKQMLSELMRQQNQHPEKPASKEGGSGTSHPPSPSLFCPFNFPTQPVNLFNLPGFTNFSSFAPSMNFSPLFPSNFGDFSQNISTPTEQQQPLAQNPSGKTEYMAFPKPFESSSSIGAEKQRNQKQPEEEVENSRTPWFYDQEGEVEKPFLKTGFAVSVEKTTNSNRKNQLDTSRRRRQFDEVSLESFNSMLDPVDPTTVTKTFKSRKASAQASLASKDKTPKSKSKKRHSTQLKSRVKNIGYESASVSSTCEPCKSRNRHSAQTEEPVQAKVLSRKNHEQLEKVIRCSRSTEISSAHARRILQQSNRNACNEAPETGSDFSMFEALRDTIYSEVATLISQNESRPHFLIELFHELQLLNTDYLRQRALYALQDIVSRHISENHEKEGENIKSVNSGTWIASNSELTPSESLATTDDETFEKNFERETHKISEQNDADNASVSSTCEPFATDVLGDTVIHLDQALARMRDYTHMKMEAESSPDVRCTCRIIEDDDGAAATTTISNNLEETPVIENHSSQQPVSEVATVPCPRIDTQQLDRQIKAIMKEVIPFLKEHMDEVCSSQLLTSVRRMVLVLTQQNNESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSVTVKQRCKRKIEAAGVIQSYAKEAKRILEGDHSSPAGEIDDEDKDKDETETVKQTQTSEVYDGDGPKNVRSDVSDQEDDEESEECPVSINLSKAETQALTNYGSGEDENEDEEIEEFEEGPVDVQTSLQANTETTEENEHDDQVPQHDFEKSSESKNVPSEQEPTTSKGDQNSTPVKPCYLSIVENEQPLNSPVQKDTLTTIDSSSQPNPLPLPLTEIETLVPRVKEVKSAQETPESSLAGSPDTESPVLVNDYEAESGNISQKSDEEDFVKVEDLPLKLTIYSEADLRKKMAEEEQKNHLSGEICAMQTEELAGNSQTLKEPEQVCAQSAILRWKPLPVPFKLLVVSSGNYCSKVSFFSNNFILKLKVLNGREQY

Target ORF information:

RefSeq Version	XM_006080142.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis pericentriolar material 1 (PCM1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006080142.1

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
AGCAATGAGA GTGTTGATGA CCGACTCAAC AATATGGATT GGGGCAGCCA GCAGAAGAAG 
GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA 
ACTAACGATA TTTCTCCGGA GTCATCACCG GGAGTTGGAA GACGAAGAAC AAAGACTTCA 
CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TCCCCGAGCA GGCAGAACTA 
GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGATTAATAC TAACAAGAGC 
AAAGATGCCA CTGTAAGTCC TCAAAAGAGA GAAATGATTG GATCAGCACA GTGTAAAGAG 
TTGTTTGCTT CTGCTTTAAG TAATGACCTT TTGCAAAACT GTCAAGTCTC TGAAGAAGAT 
GGAAGAGGAG AACCTGCAAT GGAGAGCAGC CAGATTGTAA GCAGGCTTGT TCAGATTCGT 
GAGTATATTA CTAAAGCTAG TTCCATGCGG GAAGACCTTG TAGAGAAAAA TGAAAGATCT 
GCTAATGTTG AGCGCCTCAC TCATCTAATA GATCACCTTA AAGAACAAGA AAAGTCATAT 
ATGAAATTTC TCCAGAAGAT TCTTGCCAGA GATCCTCAGC AGGAGCCTAT GGAAGAGATA 
GAAAATTTGA AGAAACAACA TGATTTATTA AAAAGGATGT TACAGCAGCA GGAGCAGCTG 
AGAGCTCTAC AAGGACGACA AGCTGCTCTG CTTGCCCTGC AGCATAAAGC TGAGCAGGCT 
ATAGCTGTGA TGGATGATTC TGAAAAGGTT GCGGAGACAA CTCCTGGCCA TCATACTGTA 
CCAGGCAGAC AGCCGGCTCA TTCACCTTTG CATCAGAGAG TACCCATAAG AGAAACTACA 
GGTAGCCTAT CTGGAGTCAG TATCACCTCT GAACTAAACG AAGAATTGAA TGATTTAATT 
CAGCATTTTC ATAATCAGCT TCGTGATTCT CAGCCTCCAG CTGTTCCAGA CAATAGAAGA 
CAAGCAGAAA GTCTTTCATT AACTAGGGAG ATTTCTCAGA GCAGAAATCC TTCAGTTTCA 
GAGCATTTAC CTGATGAGAA AGTCCAACTT TTTAGCAAGA TGAGAGTGTT ACAAGAAAAG 
AAACAAAAAA TGGACAAACT TCTTGGAGAA CTTCATACAC TTCGAGATCA GCATTTGAAC 
AATTCATCAT TTGTGCCTTC TTCAGCCTCT CCACAAAGGA GTGTGGATCA GAGAAGTACA 
ACCACAGCTC CTTCTGCTCC TGTAGTCTTA ACACCAGTGG TCAATGGAGA ATCCAATAAC 
CTCACGCCAT CTGTTCCTTA TCCTGCTGCT TCTCTAGTTT CTCAGAATCA GAATGAAAAT 
GAAGGCCACC TAAATCCAAC TGAAAAACTC CAGAAGTTAA ATGAAGTTCG AAAGAGGTTG 
AATGAACTAA GAGAATTAGT TCATTATTAT GAGCAAACAT CAGATATGAT GACAGATGCT 
GTGAATGAAA ACACAAAAGA AGAAGAAACT GAAGAATCAG AATATGATTC GGAGCATGAA 
AACTCGGAAC CTGTTACTAA CATTCGAAAT CCACAAGTGG CTGCCACCTG GAACGAAGTA 
AATAGTAATT CTAATGCACA GTGTGTTTCC AACAATAGAG ATGGGCGAGC AGTTAATTCT 
AACTGTGAAA TTAACAACAG GTCTGCTGCC AACATAAGGG CTCTAAACAT GCCTCCTCTT 
TCAGCAGATT GTCGATACAA TAGAGAAGGA GAACAGGAGA TGCCTGTTGC ACAAGGTCAT 
GAGGAGGAGG AGGAGGAGGA GGAGGAAGCA GAAGATGAGG CCCTCAGTGG AGCTTCACTG 
TCTAGTCACA GCAGCAGCCT GATCATTGAG ACTCCTGAAG ATGCTGAATT TGAACATAAG 
ATCCAGAAGC TTATGGCTGC AAAACTGAAA CTTAGACACT TACAGAACCT TGTAGCCATG 
GTGCAGGACG ATGATGCAGC CAATCAAGGA GTTACTTCTG TGGAGGATTT CTTCCCAGCA 
GAAGACACCA AACAAAATGC AAATAACACT AGAGGAAATG CCAATAAAAC ACAGAAAGAT 
GCTGGAGTAA ATGAAAAGGC AAGGGAGAAA TTTTATGAGG CTAAACTACA GCAGCAACAG 
AGAGAACTCA AACAATTGCA GGAAGAAAGA AAGAAACTGA TTGAGATTCA AGAGAAAATT 
CAAGAATTGC AAAAGGCATG TCCTAACCTA CAGCTGTCAG CTGCTAGTGT GGGTAATTGT 
CCCACAAAAA CACATATGCC AGCTGTTACT TCATCCCCAA CCGTTAATGA AAATGAAGCC 
AGTACAAACA AATCTGGTTT TGAGCCTCAT GATTCTTCAG TGGTAGATAA TGAGGTATGG 
TCAGATATGC GAAGACATGA AATGTTAAGG GAGGAGCTAC GACAGAGAAG AAAGCAGCTG 
GAAGCTCTGA TGGCTGAACA TCAGAGGAGG CAAGGTCTGG CTGAAACTGC ATCTCCAGTG 
GCTGTTTCAC TGAGAAGTGA TGGATCTGAG AACCTGTGTA CACCTCAGCA AAGCAGAACA 
GAAAAAACAA TGGCAACTTG GGGAGGTTCT ACCCAGTGTG CCCTAGATGA AGAAGGAGAT 
GAAGATGGTT ACCTTTCTGA AGGAATTATT CGGACAGACG AAGAGGAGGA AGAGGAGGAG 
CAAGATGCCA GTTCCAATGA TAACTTTCAT ATGTATCCTC CAAACAGTGC AAATCATAAT 
TCTTACAATG TAAAGGAAAC TAAAAATAGG TGGAAGAACA ATCGCCCTTT TTCAGCAGAT 
GGAAATTATC GTCCTTTAGC CAAGACAAGA CAGCAGAATA TCAGCATGCA GCGTCAGGAA 
AACCTTCGCT GGGTGTCCGA ACTCTCTTAT GTAGAAGAGA AAGAACAATG GCAAGAACAA 
ATTAATCAGC TAAAGAAACA ACTTGATTTC AGTGTCAATA TTTGTCAGAC TTTAATGCAA 
GACCAGCAGA CTCTGTCTTG TCTGCTACAA ACTCTTCTCA CGAGTCCTTA TAGTGTTATG 
CCCAGCAGTG TTGCATCCCC GCAAGTGCAC CTTATAATGC ACCAGTTGAA TCAGTGCTAT 
ACCCAGCTAA CGTGGCAGCA GAACAATGTC CAGAGGTTGA AACAAATGCT CAGTGAACTC 
ATGCGCCAAC AAAATCAGCA TCCAGAAAAA CCTGCAAGCA AGGAAGGAGG CAGTGGTACA 
TCACACCCTC CTTCTCCCAG TTTATTTTGT CCTTTCAACT TTCCAACTCA GCCTGTAAAT 
CTGTTTAACC TACCGGGGTT TACTAATTTT TCGTCATTTG CACCAAGTAT GAATTTCAGC 
CCTTTATTTC CTTCTAATTT TGGAGATTTT TCTCAAAATA TTTCTACACC CACTGAACAG 
CAGCAACCGT TAGCCCAGAA TCCTTCAGGG AAAACGGAAT ACATGGCTTT TCCAAAACCT 
TTTGAAAGTA GTTCTTCTAT TGGAGCGGAA AAACAAAGGA ATCAAAAACA GCCTGAAGAG 
GAGGTGGAGA ACAGTAGGAC ACCATGGTTT TATGACCAAG AAGGTGAAGT AGAAAAGCCA 
TTTCTCAAGA CTGGATTTGC AGTGTCTGTA GAGAAAACAA CAAATAGTAA TCGCAAAAAT 
CAACTAGATA CCAGTAGGAG AAGACGCCAG TTTGATGAAG TGTCCTTAGA AAGCTTTAAC 
AGTATGCTTG ACCCAGTAGA TCCAACAACA GTAACTAAAA CATTCAAGTC AAGAAAAGCA 
TCTGCACAGG CCAGCCTGGC ATCTAAAGAT AAAACTCCCA AATCAAAGAG TAAGAAGAGG 
CATTCTACTC AGCTGAAAAG CAGAGTGAAA AATATCGGGT ATGAAAGTGC CAGTGTGTCT 
AGCACATGTG AACCTTGCAA AAGTAGAAAC AGACATTCAG CCCAAACTGA AGAGCCTGTT 
CAAGCAAAAG TACTCAGCAG AAAGAATCAT GAGCAGCTGG AAAAAGTCAT AAGATGTAGT 
AGGTCTACAG AAATATCTTC AGCACATGCT AGGAGAATAC TACAGCAGTC TAACAGAAAT 
GCATGCAATG AAGCGCCAGA AACCGGGAGT GATTTTTCCA TGTTCGAAGC TTTGCGGGAT 
ACCATTTATT CTGAAGTAGC TACGTTGATT TCTCAAAATG AATCTCGTCC ACATTTTCTT 
ATTGAACTCT TCCATGAGCT TCAGCTACTT AATACAGACT ATTTGAGACA GAGGGCCTTA 
TATGCATTGC AGGACATAGT ATCCAGGCAT ATTTCTGAGA ACCATGAAAA AGAAGGAGAA 
AATATAAAGT CCGTGAATTC TGGTACTTGG ATAGCATCCA ACTCAGAACT TACTCCCAGT 
GAAAGCCTTG CTACTACTGA TGATGAAACT TTTGAGAAGA ACTTTGAGAG AGAAACACAT 
AAAATAAGTG AGCAAAATGA TGCTGATAAT GCTAGTGTAT CTTCAACTTG TGAGCCTTTT 
GCAACAGATG TTCTGGGCGA CACTGTGATT CACTTAGATC AGGCATTAGC CAGAATGAGA 
GACTATACGC ATATGAAGAT GGAGGCTGAA AGTAGTCCAG ACGTCAGGTG CACCTGCAGG 
ATCATTGAGG ATGACGATGG TGCTGCTGCC ACAACCACGA TTAGTAATAA TTTAGAAGAA 
ACTCCTGTTA TTGAAAATCA TAGTTCACAG CAACCCGTGA GTGAAGTTGC TACCGTCCCG 
TGTCCTAGAA TTGATACTCA GCAACTGGAC CGGCAAATTA AAGCAATTAT GAAAGAAGTC 
ATTCCTTTTT TGAAGGAGCA CATGGATGAA GTATGTTCTT CTCAACTTCT AACCTCAGTA 
AGACGCATGG TTTTGGTCCT TACTCAGCAA AACAATGAGA GCAAAGAGTT TGTAAAGTTC 
TTTCATAAGC AGCTTGGAAG TATATTACAG GATTCACTCG CAAAATTTGC TGGCAGAAAA 
CTGAAGGATT GTGGAGAAGA TCTTCTTGTA GAGATATCTG AAGTGTTATT TAATGAATTG 
GCTTTCTTTA AGCTCATGCA AGATTTGGAT AATAATAGTG TAACTGTTAA ACAGAGATGC 
AAAAGGAAAA TAGAAGCTGC TGGAGTGATA CAGTCTTATG CAAAAGAGGC CAAAAGGATT 
CTTGAAGGAG ATCACAGTTC ACCTGCTGGA GAAATTGATG ATGAAGACAA AGACAAGGAT 
GAGACGGAAA CAGTTAAGCA GACTCAAACG TCTGAGGTTT ATGATGGTGA CGGCCCCAAA 
AATGTGAGAT CTGATGTTTC TGATCAAGAG GATGATGAAG AAAGTGAAGA ATGTCCAGTG 
TCTATTAATT TGTCCAAAGC TGAAACTCAG GCTTTGACTA ATTACGGAAG TGGAGAAGAT 
GAGAATGAAG ATGAAGAAAT AGAAGAATTT GAAGAGGGAC CTGTAGATGT CCAGACGTCA 
CTTCAGGCTA ACACTGAAAC TACAGAAGAA AATGAACACG ATGATCAGGT TCCACAGCAT 
GATTTTGAAA AATCATCAGA AAGCAAAAAT GTCCCATCAG AACAAGAACC CACTACTAGT 
AAAGGTGACC AGAATAGCAC TCCTGTGAAG CCCTGTTACC TCAGTATCGT GGAAAATGAG 
CAACCTTTAA ATAGCCCTGT CCAGAAGGAC ACTCTCACTA CCATCGACTC ATCCAGTCAG 
CCTAACCCTT TGCCTTTACC TTTAACTGAA ATTGAAACCT TGGTGCCTAG AGTCAAAGAA 
GTGAAATCTG CTCAGGAGAC TCCTGAAAGT TCTTTGGCTG GAAGTCCTGA TACTGAATCT 
CCAGTGTTAG TGAATGACTA TGAAGCAGAA TCTGGTAACA TAAGTCAAAA ATCTGATGAA 
GAAGACTTTG TGAAAGTTGA AGATTTACCA CTTAAACTGA CAATATATTC AGAGGCAGAT 
CTAAGGAAGA AAATGGCCGA AGAAGAACAG AAAAACCACT TATCTGGTGA AATATGTGCA 
ATGCAGACTG AAGAATTAGC TGGGAATTCT CAGACACTAA AAGAACCTGA GCAAGTATGT 
GCTCAGTCTG CCATCTTAAG ATGGAAACCC TTACCTGTGC CTTTTAAACT GTTGGTGGTG 
TCATCAGGGA ATTATTGTAG CAAAGTAAGT TTCTTTTCTA ATAACTTTAT ATTAAAATTG 
AAAGTTTTAA ATGGAAGAGA ACAATATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( XM_025279405.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025135190.1 Bubalus bubalis pericentriolar material 1 (PCM1), transcript variant X1, mRNA.

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CloneID	OBu42290
Clone ID Related Accession (Same CDS sequence)	XM_025279405.1 , XM_025279407.1
Accession Version	XM_025279405.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6687bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	pericentriolar material 1 protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037545.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG GAGGAGGTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
AGCAATGAGA GTGTTGATGA CCGACTCAAC AATATGGATT GGGGCAGCCA GCAGAAGAAG 
GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA 
ACTAACGATA TTTCTCCGGA GTCATCACCG GGAGTTGGAA GACGAAGAAC AAAGACTTCA 
CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TCCCCGAGCA GGCAGAACTA 
GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCTCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCCAC TGTAAGTCCT 
CAAAAGAGAG AAATGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAGATTCGTG AGTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGACCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTCACT 
CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT CCAGAAGATT 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCGGT CTGAGGCTTC AGATGCCACG 
GAGGCATCTT TTAGTCTGAG AATTCGGCCG TGCGTTGAGG ACAAACTAGG GAATTCAGCT 
TCACAAGCAC AGGTTTCAGA CACTGACGTT ACTACAAGTC CAAAAGGGAA AGGTGACAGA 
CCTCCTCAGC ATGACAGGGA GCTGAGGCCT AATAGGAAAT GTAGCCGAAA ACGTGGATTT 
CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATGGAAG AGATAGAAAA TTTGAAGAAA 
CAACATGATT TATTAAAAAG GATGTTACAG CAGCAGGAGC AGCTGAGAGC TCTACAAGGA 
CGACAAGCTG CTCTGCTTGC CCTGCAGCAT AAAGCTGAGC AGGCTATAGC TGTGATGGAT 
GATTCTGAAA AGGTTGCGGA GACAACTCCT GGCCATCATA CTGTACCAGG CAGACAGCCG 
GCTCATTCAC CTTTGCATCA GAGAGTACCC ATAAGAGAAA CTACAGGTAG CCTATCTGGA 
GTCAGTATCA CCTCTGAACT AAACGAAGAA TTGAATGATT TAATTCAGCA TTTTCATAAT 
CAGCTTCGTG ATTCTCAGCC TCCAGCTGTT CCAGACAATA GAAGACAAGC AGAAAGTCTT 
TCATTAACTA GGGAGATTTC TCAGAGCAGA AATCCTTCAG TTTCAGAGCA TTTACCTGAT 
GAGAAAGTCC AACTTTTTAG CAAGATGAGA GTGTTACAAG AAAAGAAACA AAAAATGGAC 
AAACTTCTTG GAGAACTTCA TACACTTCGA GATCAGCATT TGAACAATTC ATCATTTGTG 
CCTTCTTCAG CCTCTCCACA AAGGAGTGTG GATCAGAGAA GTACAACCAC AGCTCCTTCT 
GCTCCTGTAG TCTTAACACC AGTGGTCAAT GGAGAATCCA ATAACCTCAC GCCATCTGTT 
CCTTATCCTG CTGCTTCTCT AGTTTCTCAG AATCAGAATG AAAATGAAGG CCACCTAAAT 
CCAACTGAAA AACTCCAGAA GTTAAATGAA GTTCGAAAGA GGTTGAATGA ACTAAGAGAA 
TTAGTTCATT ATTATGAGCA AACATCAGAT ATGATGACAG ATGCTGTGAA TGAAAACACA 
AAAGAAGAAG AAACTGAAGA ATCAGAATAT GATTCGGAGC ATGAAAACTC GGAACCTGTT 
ACTAACATTC GAAATCCACA AGTGGCTGCC ACCTGGAACG AAGTAAATAG TAATTCTAAT 
GCACAGTGTG TTTCCAACAA TAGAGATGGG CGAGCAGTTA ATTCTAACTG TGAAATTAAC 
AACAGGTCTG CTGCCAACAT AAGGGCTCTA AACATGCCTC CTCTTTCAGC AGATTGTCGA 
TACAATAGAG AAGGAGAACA GGAGATGCCT GTTGCACAAG GTCATGAGGA GGAGGAGGAG 
GAGGAGGAGG AAGCAGAAGA TGAGGCCCTC AGTGGAGCTT CACTGTCTAG TCACAGCAGC 
AGCCTGATCA TTGAGACTCC TGAAGATGCT GAATTTGAAC ATAAGATCCA GAAGCTTATG 
GCTGCAAAAC TGAAACTTAG ACACTTACAG AACCTTGTAG CCATGGTGCA GGACGATGAT 
GCAGCCAATC AAGGAGTTAC TTCTGTGGAG GATTTCTTCC CAGCAGAAGA CACCAAACAA 
AATGCAAATA ACACTAGAGG AAATGCCAAT AAAACACAGA AAGATGCTGG AGTAAATGAA 
AAGGCAAGGG AGAAATTTTA TGAGGCTAAA CTACAGCAGC AACAGAGAGA ACTCAAACAA 
TTGCAGGAAG AAAGAAAGAA ACTGATTGAG ATTCAAGAGA AAATTCAAGA ATTGCAAAAG 
GCATGTCCTA ACCTACAGCT GTCAGCTGCT AGTGTGGGTA ATTGTCCCAC AAAAACACAT 
ATGCCAGCTG TTACTTCATC CCCAACCGTT AATGAAAATG AAGCCAGTAC AAACAAATCT 
GGTTTTGAGC CTCATGATTC TTCAGTGGTA GATAATGAGG TATGGTCAGA TATGCGAAGA 
CATGAAATGT TAAGGGAGGA GCTACGACAG AGAAGAAAGC AGCTGGAAGC TCTGATGGCT 
GAACATCAGA GGAGGCAAGG TCTGGCTGAA ACTGCATCTC CAGTGGCTGT TTCACTGAGA 
AGTGATGGAT CTGAGAACCT GTGTACACCT CAGCAAAGCA GAACAGAAAA AACAATGGCA 
ACTTGGGGAG GTTCTACCCA GTGTGCCCTA GATGAAGAAG GAGATGAAGA TGGTTACCTT 
TCTGAAGGAA TTATTCGGAC AGACGAAGAG GAGGAAGAGG AGGAGCAAGA TGCCAGTTCC 
AATGATAACT TTCATATGTA TCCTCCAAAC AGTGCAAATC ATAATTCTTA CAATGTAAAG 
GAAACTAAAA ATAGGTGGAA GAACAATCGC CCTTTTTCAG CAGATGGAAA TTATCGTCCT 
TTAGCCAAGA CAAGACAGCA GAATATCAGC ATGCAGCGTC AGGAAAACCT TCGCTGGGTG 
TCCGAACTCT CTTATGTAGA AGAGAAAGAA CAATGGCAAG AACAAATTAA TCAGCTAAAG 
AAACAACTTG ATTTCAGTGT CAATATTTGT CAGACTTTAA TGCAAGACCA GCAGACTCTG 
TCTTGTCTGC TACAAACTCT TCTCACGAGT CCTTATAGTG TTATGCCCAG CAGTGTTGCA 
TCCCCGCAAG TGCACCTTAT AATGCACCAG TTGAATCAGT GCTATACCCA GCTAACGTGG 
CAGCAGAACA ATGTCCAGAG GTTGAAACAA ATGCTCAGTG AACTCATGCG CCAACAAAAT 
CAGCATCCAG AAAAACCTGC AAGCAAGGAA GGAGGCAGTG GTACATCACA CCCTCCTTCT 
CCCAGTTTAT TTTGTCCTTT CAACTTTCCA ACTCAGCCTG TAAATCTGTT TAACCTACCG 
GGGTTTACTA ATTTTTCGTC ATTTGCACCA AGTATGAATT TCAGCCCTTT ATTTCCTTCT 
AATTTTGGAG ATTTTTCTCA AAATATTTCT ACACCCACTG AACAGCAGCA ACCGTTAGCC 
CAGAATCCTT CAGGGAAAAC GGAATACATG GCTTTTCCAA AACCTTTTGA AAGTAGTTCT 
TCTATTGGAG CGGAAAAACA AAGGAATCAA AAACAGCCTG AAGAGGAGGT GGAGAACAGT 
AGGACACCAT GGTTTTATGA CCAAGAAGGT GAAGTAGAAA AGCCATTTCT CAAGACTGGA 
TTTGCAGTGT CTGTAGAGAA AACAACAAAT AGTAATCGCA AAAATCAACT AGATACCAGT 
AGGAGAAGAC GCCAGTTTGA TGAAGTGTCC TTAGAAAGCT TTAACAGTAT GCTTGACCCA 
GTAGATCCAA CAACAGTAAC TAAAACATTC AAGTCAAGAA AAGCATCTGC ACAGGCCAGC 
CTGGCATCTA AAGATAAAAC TCCCAAATCA AAGAGTAAGA AGAGGCATTC TACTCAGCTG 
AAAAGCAGAG TGAAAAATAT CGGGTATGAA AGTGCCAGTG TGTCTAGCAC ATGTGAACCT 
TGCAAAAGTA GAAACAGACA TTCAGCCCAA ACTGAAGAGC CTGTTCAAGC AAAAGTACTC 
AGCAGAAAGA ATCATGAGCA GCTGGAAAAA GTCATAAGAT GTAGTAGGTC TACAGAAATA 
TCTTCAGCAC ATGCTAGGAG AATACTACAG CAGTCTAACA GAAATGCATG CAATGAAGCG 
CCAGAAACCG GGAGTGATTT TTCCATGTTC GAAGCTTTGC GGGATACCAT TTATTCTGAA 
GTAGCTACGT TGATTTCTCA AAATGAATCT CGTCCACATT TTCTTATTGA ACTCTTCCAT 
GAGCTTCAGC TACTTAATAC AGACTATTTG AGACAGAGGG CCTTATATGC ATTGCAGGAC 
ATAGTATCCA GGCATATTTC TGAGAACCAT GAAAAAGAAG GAGAAAATAT AAAGTCCGTG 
AATTCTGGTA CTTGGATAGC ATCCAACTCA GAACTTACTC CCAGTGAAAG CCTTGCTACT 
ACTGATGATG AAACTTTTGA GAAGAACTTT GAGAGAGAAA CACATAAAAT AAGTGAGCAA 
AATGATGCTG ATAATGCTAG TGTATCTTCA ACTTGTGAGC CTTTTGCAAC AGATGTTCTG 
GGCGACACTG TGATTCACTT AGATCAGGCA TTAGCCAGAA TGAGAGACTA TACGCATATG 
AAGATGGAGG CTGAAAGTAG TCCAGACGTC AGGTGCACCT GCAGGATCAT TGAGGATGAC 
GATGGTGCTG CTGCCACAAC CACGATTAGT AATAATTTAG AAGAAACTCC TGTTATTGAA 
AATCATAGTT CACAGCAACC CGTGAGTGAA GTTGCTACCG TCCCGTGTCC TAGAATTGAT 
ACTCAGCAAC TGGACCGGCA AATTAAAGCA ATTATGAAAG AAGTCATTCC TTTTTTGAAG 
GAGCACATGG ATGAAGTATG TTCTTCTCAA CTTCTAACCT CAGTAAGACG CATGGTTTTG 
GTCCTTACTC AGCAAAACAA TGAGAGCAAA GAGTTTGTAA AGTTCTTTCA TAAGCAGCTT 
GGAAGTATAT TACAGGATTC ACTCGCAAAA TTTGCTGGCA GAAAACTGAA GGATTGTGGA 
GAAGATCTTC TTGTAGAGAT ATCTGAAGTG TTATTTAATG AATTGGCTTT CTTTAAGCTC 
ATGCAAGATT TGGATAATAA TAGTGTAACT GTTAAACAGA GATGCAAAAG GAAAATAGAA 
GCTGCTGGAG TGATACAGTC TTATGCAAAA GAGGCCAAAA GGATTCTTGA AGGAGATCAC 
AGTTCACCTG CTGGAGAAAT TGATGATGAA GACAAAGACA AGGATGAGAC GGAAACAGTT 
AAGCAGACTC AAACGTCTGA GGTTTATGAT GGTGACGGCC CCAAAAATGT GAGATCTGAT 
GTTTCTGATC AAGAGGATGA TGAAGAAAGT GAAGAATGTC CAGTGTCTAT TAATTTGTCC 
AAAGCTGAAA CTCAGGCTTT GACTAATTAC GGAAGTGGAG AAGATGAGAA TGAAGATGAA 
GAAATAGAAG AATTTGAAGA GGGACCTGTA GATGTCCAGA CGTCACTTCA GGCTAACACT 
GAAACTACAG AAGAAAATGA ACACGATGAT CAGGTTCCAC AGCATGATTT TGAAAAATCA 
TCAGAAAGCA AAAATGTCCC ATCAGAACAA GAACCCACTA CTAGTAAAGA CTTTCTTTTG 
ATGACAGGTG ACCAGAATAG CACTCCTGTG AAGCCCTGTT ACCTCAGTAT CGTGGAAAAT 
GAGCAACCTT TAAATAGCCC TGTCCAGAAG GACACTCTCA CTACCATCGA CTCATCCAGT 
CAGCCTAACC CTTTGCCTTT ACCTTTAACT GAAATTGAAA CCTTGGTGCC TAGAGTCAAA 
GAAGTGAAAT CTGCTCAGGA GACTCCTGAA AGTTCTTTGG CTGGAAGTCC TGATACTGAA 
TCTCCAGTGT TAGTGAATGA CTATGAAGCA GAATCTGGTA ACATAAGTCA AAAATCTGAT 
GAAGAAGACT TTGTGAAAGT TGAAGATTTA CCACTTAAAC TGACAATATA TTCAGAGGCA 
GATCTAAGGA AGAAAATGGC CGAAGAAGAA CAGAAAAACC ACTTATCTGG TGAAATATGT 
GCAATGCAGA CTGAAGAATT AGCTGGGAAT TCTCAGACAC TAAAAGAACC TGAGCAAGTA 
TGTGCTCAGT CTGCCATCTT AAGATGGAAA CCCTTACCTG TGCCTTTTAA ACTGTTGGTG 
GTGTCATCAG GGAATTATTG TAGCAAAAAA CAGTGGGAGC CCAAAGTACA TGAGATGTCT 
CCAGAGGCTC AGCTAACTCT TTCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025135190.1
CDS	392..7078
Translation	MATGGGPFEEGVNDQDLPNWSNESVDDRLNNMDWGSQQKKANRSSEKNKRKFGVESDKRVTNDISPESSPGVGRRRTKTSLTFPHSRYMTQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDATVSPQKREMIGSAQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIREYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLQKILARENEEEDVRTIDSAVGSGSVAESTSLNIDVRSEASDATEASFSLRIRPCVEDKLGNSASQAQVSDTDVTTSPKGKGDRPPQHDRELRPNRKCSRKRGFPSKARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSEKVAETTPGHHTVPGRQPAHSPLHQRVPIRETTGSLSGVSITSELNEELNDLIQHFHNQLRDSQPPAVPDNRRQAESLSLTREISQSRNPSVSEHLPDEKVQLFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSFVPSSASPQRSVDQRSTTTAPSAPVVLTPVVNGESNNLTPSVPYPAASLVSQNQNENEGHLNPTEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENTKEEETEESEYDSEHENSEPVTNIRNPQVAATWNEVNSNSNAQCVSNNRDGRAVNSNCEINNRSAANIRALNMPPLSADCRYNREGEQEMPVAQGHEEEEEEEEEAEDEALSGASLSSHSSSLIIETPEDAEFEHKIQKLMAAKLKLRHLQNLVAMVQDDDAANQGVTSVEDFFPAEDTKQNANNTRGNANKTQKDAGVNEKAREKFYEAKLQQQQRELKQLQEERKKLIEIQEKIQELQKACPNLQLSAASVGNCPTKTHMPAVTSSPTVNENEASTNKSGFEPHDSSVVDNEVWSDMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETASPVAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGIIRTDEEEEEEEQDASSNDNFHMYPPNSANHNSYNVKETKNRWKNNRPFSADGNYRPLAKTRQQNISMQRQENLRWVSELSYVEEKEQWQEQINQLKKQLDFSVNICQTLMQDQQTLSCLLQTLLTSPYSVMPSSVASPQVHLIMHQLNQCYTQLTWQQNNVQRLKQMLSELMRQQNQHPEKPASKEGGSGTSHPPSPSLFCPFNFPTQPVNLFNLPGFTNFSSFAPSMNFSPLFPSNFGDFSQNISTPTEQQQPLAQNPSGKTEYMAFPKPFESSSSIGAEKQRNQKQPEEEVENSRTPWFYDQEGEVEKPFLKTGFAVSVEKTTNSNRKNQLDTSRRRRQFDEVSLESFNSMLDPVDPTTVTKTFKSRKASAQASLASKDKTPKSKSKKRHSTQLKSRVKNIGYESASVSSTCEPCKSRNRHSAQTEEPVQAKVLSRKNHEQLEKVIRCSRSTEISSAHARRILQQSNRNACNEAPETGSDFSMFEALRDTIYSEVATLISQNESRPHFLIELFHELQLLNTDYLRQRALYALQDIVSRHISENHEKEGENIKSVNSGTWIASNSELTPSESLATTDDETFEKNFERETHKISEQNDADNASVSSTCEPFATDVLGDTVIHLDQALARMRDYTHMKMEAESSPDVRCTCRIIEDDDGAAATTTISNNLEETPVIENHSSQQPVSEVATVPCPRIDTQQLDRQIKAIMKEVIPFLKEHMDEVCSSQLLTSVRRMVLVLTQQNNESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSVTVKQRCKRKIEAAGVIQSYAKEAKRILEGDHSSPAGEIDDEDKDKDETETVKQTQTSEVYDGDGPKNVRSDVSDQEDDEESEECPVSINLSKAETQALTNYGSGEDENEDEEIEEFEEGPVDVQTSLQANTETTEENEHDDQVPQHDFEKSSESKNVPSEQEPTTSKDFLLMTGDQNSTPVKPCYLSIVENEQPLNSPVQKDTLTTIDSSSQPNPLPLPLTEIETLVPRVKEVKSAQETPESSLAGSPDTESPVLVNDYEAESGNISQKSDEEDFVKVEDLPLKLTIYSEADLRKKMAEEEQKNHLSGEICAMQTEELAGNSQTLKEPEQVCAQSAILRWKPLPVPFKLLVVSSGNYCSKKQWEPKVHEMSPEAQLTLS

Target ORF information:

RefSeq Version	XM_025279405.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis pericentriolar material 1 (PCM1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025279405.1

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
AGCAATGAGA GTGTTGATGA CCGACTCAAC AATATGGATT GGGGCAGCCA GCAGAAGAAG 
GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA 
ACTAACGATA TTTCTCCGGA GTCATCACCG GGAGTTGGAA GACGAAGAAC AAAGACTTCA 
CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TCCCCGAGCA GGCAGAACTA 
GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCTCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCCAC TGTAAGTCCT 
CAAAAGAGAG AAATGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAGATTCGTG AGTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGACCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTCACT 
CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT CCAGAAGATT 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCGGT CTGAGGCTTC AGATGCCACG 
GAGGCATCTT TTAGTCTGAG AATTCGGCCG TGCGTTGAGG ACAAACTAGG GAATTCAGCT 
TCACAAGCAC AGGTTTCAGA CACTGACGTT ACTACAAGTC CAAAAGGGAA AGGTGACAGA 
CCTCCTCAGC ATGACAGGGA GCTGAGGCCT AATAGGAAAT GTAGCCGAAA ACGTGGATTT 
CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATGGAAG AGATAGAAAA TTTGAAGAAA 
CAACATGATT TATTAAAAAG GATGTTACAG CAGCAGGAGC AGCTGAGAGC TCTACAAGGA 
CGACAAGCTG CTCTGCTTGC CCTGCAGCAT AAAGCTGAGC AGGCTATAGC TGTGATGGAT 
GATTCTGAAA AGGTTGCGGA GACAACTCCT GGCCATCATA CTGTACCAGG CAGACAGCCG 
GCTCATTCAC CTTTGCATCA GAGAGTACCC ATAAGAGAAA CTACAGGTAG CCTATCTGGA 
GTCAGTATCA CCTCTGAACT AAACGAAGAA TTGAATGATT TAATTCAGCA TTTTCATAAT 
CAGCTTCGTG ATTCTCAGCC TCCAGCTGTT CCAGACAATA GAAGACAAGC AGAAAGTCTT 
TCATTAACTA GGGAGATTTC TCAGAGCAGA AATCCTTCAG TTTCAGAGCA TTTACCTGAT 
GAGAAAGTCC AACTTTTTAG CAAGATGAGA GTGTTACAAG AAAAGAAACA AAAAATGGAC 
AAACTTCTTG GAGAACTTCA TACACTTCGA GATCAGCATT TGAACAATTC ATCATTTGTG 
CCTTCTTCAG CCTCTCCACA AAGGAGTGTG GATCAGAGAA GTACAACCAC AGCTCCTTCT 
GCTCCTGTAG TCTTAACACC AGTGGTCAAT GGAGAATCCA ATAACCTCAC GCCATCTGTT 
CCTTATCCTG CTGCTTCTCT AGTTTCTCAG AATCAGAATG AAAATGAAGG CCACCTAAAT 
CCAACTGAAA AACTCCAGAA GTTAAATGAA GTTCGAAAGA GGTTGAATGA ACTAAGAGAA 
TTAGTTCATT ATTATGAGCA AACATCAGAT ATGATGACAG ATGCTGTGAA TGAAAACACA 
AAAGAAGAAG AAACTGAAGA ATCAGAATAT GATTCGGAGC ATGAAAACTC GGAACCTGTT 
ACTAACATTC GAAATCCACA AGTGGCTGCC ACCTGGAACG AAGTAAATAG TAATTCTAAT 
GCACAGTGTG TTTCCAACAA TAGAGATGGG CGAGCAGTTA ATTCTAACTG TGAAATTAAC 
AACAGGTCTG CTGCCAACAT AAGGGCTCTA AACATGCCTC CTCTTTCAGC AGATTGTCGA 
TACAATAGAG AAGGAGAACA GGAGATGCCT GTTGCACAAG GTCATGAGGA GGAGGAGGAG 
GAGGAGGAGG AAGCAGAAGA TGAGGCCCTC AGTGGAGCTT CACTGTCTAG TCACAGCAGC 
AGCCTGATCA TTGAGACTCC TGAAGATGCT GAATTTGAAC ATAAGATCCA GAAGCTTATG 
GCTGCAAAAC TGAAACTTAG ACACTTACAG AACCTTGTAG CCATGGTGCA GGACGATGAT 
GCAGCCAATC AAGGAGTTAC TTCTGTGGAG GATTTCTTCC CAGCAGAAGA CACCAAACAA 
AATGCAAATA ACACTAGAGG AAATGCCAAT AAAACACAGA AAGATGCTGG AGTAAATGAA 
AAGGCAAGGG AGAAATTTTA TGAGGCTAAA CTACAGCAGC AACAGAGAGA ACTCAAACAA 
TTGCAGGAAG AAAGAAAGAA ACTGATTGAG ATTCAAGAGA AAATTCAAGA ATTGCAAAAG 
GCATGTCCTA ACCTACAGCT GTCAGCTGCT AGTGTGGGTA ATTGTCCCAC AAAAACACAT 
ATGCCAGCTG TTACTTCATC CCCAACCGTT AATGAAAATG AAGCCAGTAC AAACAAATCT 
GGTTTTGAGC CTCATGATTC TTCAGTGGTA GATAATGAGG TATGGTCAGA TATGCGAAGA 
CATGAAATGT TAAGGGAGGA GCTACGACAG AGAAGAAAGC AGCTGGAAGC TCTGATGGCT 
GAACATCAGA GGAGGCAAGG TCTGGCTGAA ACTGCATCTC CAGTGGCTGT TTCACTGAGA 
AGTGATGGAT CTGAGAACCT GTGTACACCT CAGCAAAGCA GAACAGAAAA AACAATGGCA 
ACTTGGGGAG GTTCTACCCA GTGTGCCCTA GATGAAGAAG GAGATGAAGA TGGTTACCTT 
TCTGAAGGAA TTATTCGGAC AGACGAAGAG GAGGAAGAGG AGGAGCAAGA TGCCAGTTCC 
AATGATAACT TTCATATGTA TCCTCCAAAC AGTGCAAATC ATAATTCTTA CAATGTAAAG 
GAAACTAAAA ATAGGTGGAA GAACAATCGC CCTTTTTCAG CAGATGGAAA TTATCGTCCT 
TTAGCCAAGA CAAGACAGCA GAATATCAGC ATGCAGCGTC AGGAAAACCT TCGCTGGGTG 
TCCGAACTCT CTTATGTAGA AGAGAAAGAA CAATGGCAAG AACAAATTAA TCAGCTAAAG 
AAACAACTTG ATTTCAGTGT CAATATTTGT CAGACTTTAA TGCAAGACCA GCAGACTCTG 
TCTTGTCTGC TACAAACTCT TCTCACGAGT CCTTATAGTG TTATGCCCAG CAGTGTTGCA 
TCCCCGCAAG TGCACCTTAT AATGCACCAG TTGAATCAGT GCTATACCCA GCTAACGTGG 
CAGCAGAACA ATGTCCAGAG GTTGAAACAA ATGCTCAGTG AACTCATGCG CCAACAAAAT 
CAGCATCCAG AAAAACCTGC AAGCAAGGAA GGAGGCAGTG GTACATCACA CCCTCCTTCT 
CCCAGTTTAT TTTGTCCTTT CAACTTTCCA ACTCAGCCTG TAAATCTGTT TAACCTACCG 
GGGTTTACTA ATTTTTCGTC ATTTGCACCA AGTATGAATT TCAGCCCTTT ATTTCCTTCT 
AATTTTGGAG ATTTTTCTCA AAATATTTCT ACACCCACTG AACAGCAGCA ACCGTTAGCC 
CAGAATCCTT CAGGGAAAAC GGAATACATG GCTTTTCCAA AACCTTTTGA AAGTAGTTCT 
TCTATTGGAG CGGAAAAACA AAGGAATCAA AAACAGCCTG AAGAGGAGGT GGAGAACAGT 
AGGACACCAT GGTTTTATGA CCAAGAAGGT GAAGTAGAAA AGCCATTTCT CAAGACTGGA 
TTTGCAGTGT CTGTAGAGAA AACAACAAAT AGTAATCGCA AAAATCAACT AGATACCAGT 
AGGAGAAGAC GCCAGTTTGA TGAAGTGTCC TTAGAAAGCT TTAACAGTAT GCTTGACCCA 
GTAGATCCAA CAACAGTAAC TAAAACATTC AAGTCAAGAA AAGCATCTGC ACAGGCCAGC 
CTGGCATCTA AAGATAAAAC TCCCAAATCA AAGAGTAAGA AGAGGCATTC TACTCAGCTG 
AAAAGCAGAG TGAAAAATAT CGGGTATGAA AGTGCCAGTG TGTCTAGCAC ATGTGAACCT 
TGCAAAAGTA GAAACAGACA TTCAGCCCAA ACTGAAGAGC CTGTTCAAGC AAAAGTACTC 
AGCAGAAAGA ATCATGAGCA GCTGGAAAAA GTCATAAGAT GTAGTAGGTC TACAGAAATA 
TCTTCAGCAC ATGCTAGGAG AATACTACAG CAGTCTAACA GAAATGCATG CAATGAAGCG 
CCAGAAACCG GGAGTGATTT TTCCATGTTC GAAGCTTTGC GGGATACCAT TTATTCTGAA 
GTAGCTACGT TGATTTCTCA AAATGAATCT CGTCCACATT TTCTTATTGA ACTCTTCCAT 
GAGCTTCAGC TACTTAATAC AGACTATTTG AGACAGAGGG CCTTATATGC ATTGCAGGAC 
ATAGTATCCA GGCATATTTC TGAGAACCAT GAAAAAGAAG GAGAAAATAT AAAGTCCGTG 
AATTCTGGTA CTTGGATAGC ATCCAACTCA GAACTTACTC CCAGTGAAAG CCTTGCTACT 
ACTGATGATG AAACTTTTGA GAAGAACTTT GAGAGAGAAA CACATAAAAT AAGTGAGCAA 
AATGATGCTG ATAATGCTAG TGTATCTTCA ACTTGTGAGC CTTTTGCAAC AGATGTTCTG 
GGCGACACTG TGATTCACTT AGATCAGGCA TTAGCCAGAA TGAGAGACTA TACGCATATG 
AAGATGGAGG CTGAAAGTAG TCCAGACGTC AGGTGCACCT GCAGGATCAT TGAGGATGAC 
GATGGTGCTG CTGCCACAAC CACGATTAGT AATAATTTAG AAGAAACTCC TGTTATTGAA 
AATCATAGTT CACAGCAACC CGTGAGTGAA GTTGCTACCG TCCCGTGTCC TAGAATTGAT 
ACTCAGCAAC TGGACCGGCA AATTAAAGCA ATTATGAAAG AAGTCATTCC TTTTTTGAAG 
GAGCACATGG ATGAAGTATG TTCTTCTCAA CTTCTAACCT CAGTAAGACG CATGGTTTTG 
GTCCTTACTC AGCAAAACAA TGAGAGCAAA GAGTTTGTAA AGTTCTTTCA TAAGCAGCTT 
GGAAGTATAT TACAGGATTC ACTCGCAAAA TTTGCTGGCA GAAAACTGAA GGATTGTGGA 
GAAGATCTTC TTGTAGAGAT ATCTGAAGTG TTATTTAATG AATTGGCTTT CTTTAAGCTC 
ATGCAAGATT TGGATAATAA TAGTGTAACT GTTAAACAGA GATGCAAAAG GAAAATAGAA 
GCTGCTGGAG TGATACAGTC TTATGCAAAA GAGGCCAAAA GGATTCTTGA AGGAGATCAC 
AGTTCACCTG CTGGAGAAAT TGATGATGAA GACAAAGACA AGGATGAGAC GGAAACAGTT 
AAGCAGACTC AAACGTCTGA GGTTTATGAT GGTGACGGCC CCAAAAATGT GAGATCTGAT 
GTTTCTGATC AAGAGGATGA TGAAGAAAGT GAAGAATGTC CAGTGTCTAT TAATTTGTCC 
AAAGCTGAAA CTCAGGCTTT GACTAATTAC GGAAGTGGAG AAGATGAGAA TGAAGATGAA 
GAAATAGAAG AATTTGAAGA GGGACCTGTA GATGTCCAGA CGTCACTTCA GGCTAACACT 
GAAACTACAG AAGAAAATGA ACACGATGAT CAGGTTCCAC AGCATGATTT TGAAAAATCA 
TCAGAAAGCA AAAATGTCCC ATCAGAACAA GAACCCACTA CTAGTAAAGA CTTTCTTTTG 
ATGACAGGTG ACCAGAATAG CACTCCTGTG AAGCCCTGTT ACCTCAGTAT CGTGGAAAAT 
GAGCAACCTT TAAATAGCCC TGTCCAGAAG GACACTCTCA CTACCATCGA CTCATCCAGT 
CAGCCTAACC CTTTGCCTTT ACCTTTAACT GAAATTGAAA CCTTGGTGCC TAGAGTCAAA 
GAAGTGAAAT CTGCTCAGGA GACTCCTGAA AGTTCTTTGG CTGGAAGTCC TGATACTGAA 
TCTCCAGTGT TAGTGAATGA CTATGAAGCA GAATCTGGTA ACATAAGTCA AAAATCTGAT 
GAAGAAGACT TTGTGAAAGT TGAAGATTTA CCACTTAAAC TGACAATATA TTCAGAGGCA 
GATCTAAGGA AGAAAATGGC CGAAGAAGAA CAGAAAAACC ACTTATCTGG TGAAATATGT 
GCAATGCAGA CTGAAGAATT AGCTGGGAAT TCTCAGACAC TAAAAGAACC TGAGCAAGTA 
TGTGCTCAGT CTGCCATCTT AAGATGGAAA CCCTTACCTG TGCCTTTTAA ACTGTTGGTG 
GTGTCATCAG GGAATTATTG TAGCAAAAAA CAGTGGGAGC CCAAAGTACA TGAGATGTCT 
CCAGAGGCTC AGCTAACTCT TTCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( XM_025279407.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025135192.1 Bubalus bubalis pericentriolar material 1 (PCM1), transcript variant X2, mRNA.

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CloneID	OBu42290
Clone ID Related Accession (Same CDS sequence)	XM_025279405.1 , XM_025279407.1
Accession Version	XM_025279407.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6687bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	pericentriolar material 1 protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037545.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG GAGGAGGTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
AGCAATGAGA GTGTTGATGA CCGACTCAAC AATATGGATT GGGGCAGCCA GCAGAAGAAG 
GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA 
ACTAACGATA TTTCTCCGGA GTCATCACCG GGAGTTGGAA GACGAAGAAC AAAGACTTCA 
CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TCCCCGAGCA GGCAGAACTA 
GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCTCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCCAC TGTAAGTCCT 
CAAAAGAGAG AAATGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAGATTCGTG AGTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGACCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTCACT 
CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT CCAGAAGATT 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCGGT CTGAGGCTTC AGATGCCACG 
GAGGCATCTT TTAGTCTGAG AATTCGGCCG TGCGTTGAGG ACAAACTAGG GAATTCAGCT 
TCACAAGCAC AGGTTTCAGA CACTGACGTT ACTACAAGTC CAAAAGGGAA AGGTGACAGA 
CCTCCTCAGC ATGACAGGGA GCTGAGGCCT AATAGGAAAT GTAGCCGAAA ACGTGGATTT 
CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATGGAAG AGATAGAAAA TTTGAAGAAA 
CAACATGATT TATTAAAAAG GATGTTACAG CAGCAGGAGC AGCTGAGAGC TCTACAAGGA 
CGACAAGCTG CTCTGCTTGC CCTGCAGCAT AAAGCTGAGC AGGCTATAGC TGTGATGGAT 
GATTCTGAAA AGGTTGCGGA GACAACTCCT GGCCATCATA CTGTACCAGG CAGACAGCCG 
GCTCATTCAC CTTTGCATCA GAGAGTACCC ATAAGAGAAA CTACAGGTAG CCTATCTGGA 
GTCAGTATCA CCTCTGAACT AAACGAAGAA TTGAATGATT TAATTCAGCA TTTTCATAAT 
CAGCTTCGTG ATTCTCAGCC TCCAGCTGTT CCAGACAATA GAAGACAAGC AGAAAGTCTT 
TCATTAACTA GGGAGATTTC TCAGAGCAGA AATCCTTCAG TTTCAGAGCA TTTACCTGAT 
GAGAAAGTCC AACTTTTTAG CAAGATGAGA GTGTTACAAG AAAAGAAACA AAAAATGGAC 
AAACTTCTTG GAGAACTTCA TACACTTCGA GATCAGCATT TGAACAATTC ATCATTTGTG 
CCTTCTTCAG CCTCTCCACA AAGGAGTGTG GATCAGAGAA GTACAACCAC AGCTCCTTCT 
GCTCCTGTAG TCTTAACACC AGTGGTCAAT GGAGAATCCA ATAACCTCAC GCCATCTGTT 
CCTTATCCTG CTGCTTCTCT AGTTTCTCAG AATCAGAATG AAAATGAAGG CCACCTAAAT 
CCAACTGAAA AACTCCAGAA GTTAAATGAA GTTCGAAAGA GGTTGAATGA ACTAAGAGAA 
TTAGTTCATT ATTATGAGCA AACATCAGAT ATGATGACAG ATGCTGTGAA TGAAAACACA 
AAAGAAGAAG AAACTGAAGA ATCAGAATAT GATTCGGAGC ATGAAAACTC GGAACCTGTT 
ACTAACATTC GAAATCCACA AGTGGCTGCC ACCTGGAACG AAGTAAATAG TAATTCTAAT 
GCACAGTGTG TTTCCAACAA TAGAGATGGG CGAGCAGTTA ATTCTAACTG TGAAATTAAC 
AACAGGTCTG CTGCCAACAT AAGGGCTCTA AACATGCCTC CTCTTTCAGC AGATTGTCGA 
TACAATAGAG AAGGAGAACA GGAGATGCCT GTTGCACAAG GTCATGAGGA GGAGGAGGAG 
GAGGAGGAGG AAGCAGAAGA TGAGGCCCTC AGTGGAGCTT CACTGTCTAG TCACAGCAGC 
AGCCTGATCA TTGAGACTCC TGAAGATGCT GAATTTGAAC ATAAGATCCA GAAGCTTATG 
GCTGCAAAAC TGAAACTTAG ACACTTACAG AACCTTGTAG CCATGGTGCA GGACGATGAT 
GCAGCCAATC AAGGAGTTAC TTCTGTGGAG GATTTCTTCC CAGCAGAAGA CACCAAACAA 
AATGCAAATA ACACTAGAGG AAATGCCAAT AAAACACAGA AAGATGCTGG AGTAAATGAA 
AAGGCAAGGG AGAAATTTTA TGAGGCTAAA CTACAGCAGC AACAGAGAGA ACTCAAACAA 
TTGCAGGAAG AAAGAAAGAA ACTGATTGAG ATTCAAGAGA AAATTCAAGA ATTGCAAAAG 
GCATGTCCTA ACCTACAGCT GTCAGCTGCT AGTGTGGGTA ATTGTCCCAC AAAAACACAT 
ATGCCAGCTG TTACTTCATC CCCAACCGTT AATGAAAATG AAGCCAGTAC AAACAAATCT 
GGTTTTGAGC CTCATGATTC TTCAGTGGTA GATAATGAGG TATGGTCAGA TATGCGAAGA 
CATGAAATGT TAAGGGAGGA GCTACGACAG AGAAGAAAGC AGCTGGAAGC TCTGATGGCT 
GAACATCAGA GGAGGCAAGG TCTGGCTGAA ACTGCATCTC CAGTGGCTGT TTCACTGAGA 
AGTGATGGAT CTGAGAACCT GTGTACACCT CAGCAAAGCA GAACAGAAAA AACAATGGCA 
ACTTGGGGAG GTTCTACCCA GTGTGCCCTA GATGAAGAAG GAGATGAAGA TGGTTACCTT 
TCTGAAGGAA TTATTCGGAC AGACGAAGAG GAGGAAGAGG AGGAGCAAGA TGCCAGTTCC 
AATGATAACT TTCATATGTA TCCTCCAAAC AGTGCAAATC ATAATTCTTA CAATGTAAAG 
GAAACTAAAA ATAGGTGGAA GAACAATCGC CCTTTTTCAG CAGATGGAAA TTATCGTCCT 
TTAGCCAAGA CAAGACAGCA GAATATCAGC ATGCAGCGTC AGGAAAACCT TCGCTGGGTG 
TCCGAACTCT CTTATGTAGA AGAGAAAGAA CAATGGCAAG AACAAATTAA TCAGCTAAAG 
AAACAACTTG ATTTCAGTGT CAATATTTGT CAGACTTTAA TGCAAGACCA GCAGACTCTG 
TCTTGTCTGC TACAAACTCT TCTCACGAGT CCTTATAGTG TTATGCCCAG CAGTGTTGCA 
TCCCCGCAAG TGCACCTTAT AATGCACCAG TTGAATCAGT GCTATACCCA GCTAACGTGG 
CAGCAGAACA ATGTCCAGAG GTTGAAACAA ATGCTCAGTG AACTCATGCG CCAACAAAAT 
CAGCATCCAG AAAAACCTGC AAGCAAGGAA GGAGGCAGTG GTACATCACA CCCTCCTTCT 
CCCAGTTTAT TTTGTCCTTT CAACTTTCCA ACTCAGCCTG TAAATCTGTT TAACCTACCG 
GGGTTTACTA ATTTTTCGTC ATTTGCACCA AGTATGAATT TCAGCCCTTT ATTTCCTTCT 
AATTTTGGAG ATTTTTCTCA AAATATTTCT ACACCCACTG AACAGCAGCA ACCGTTAGCC 
CAGAATCCTT CAGGGAAAAC GGAATACATG GCTTTTCCAA AACCTTTTGA AAGTAGTTCT 
TCTATTGGAG CGGAAAAACA AAGGAATCAA AAACAGCCTG AAGAGGAGGT GGAGAACAGT 
AGGACACCAT GGTTTTATGA CCAAGAAGGT GAAGTAGAAA AGCCATTTCT CAAGACTGGA 
TTTGCAGTGT CTGTAGAGAA AACAACAAAT AGTAATCGCA AAAATCAACT AGATACCAGT 
AGGAGAAGAC GCCAGTTTGA TGAAGTGTCC TTAGAAAGCT TTAACAGTAT GCTTGACCCA 
GTAGATCCAA CAACAGTAAC TAAAACATTC AAGTCAAGAA AAGCATCTGC ACAGGCCAGC 
CTGGCATCTA AAGATAAAAC TCCCAAATCA AAGAGTAAGA AGAGGCATTC TACTCAGCTG 
AAAAGCAGAG TGAAAAATAT CGGGTATGAA AGTGCCAGTG TGTCTAGCAC ATGTGAACCT 
TGCAAAAGTA GAAACAGACA TTCAGCCCAA ACTGAAGAGC CTGTTCAAGC AAAAGTACTC 
AGCAGAAAGA ATCATGAGCA GCTGGAAAAA GTCATAAGAT GTAGTAGGTC TACAGAAATA 
TCTTCAGCAC ATGCTAGGAG AATACTACAG CAGTCTAACA GAAATGCATG CAATGAAGCG 
CCAGAAACCG GGAGTGATTT TTCCATGTTC GAAGCTTTGC GGGATACCAT TTATTCTGAA 
GTAGCTACGT TGATTTCTCA AAATGAATCT CGTCCACATT TTCTTATTGA ACTCTTCCAT 
GAGCTTCAGC TACTTAATAC AGACTATTTG AGACAGAGGG CCTTATATGC ATTGCAGGAC 
ATAGTATCCA GGCATATTTC TGAGAACCAT GAAAAAGAAG GAGAAAATAT AAAGTCCGTG 
AATTCTGGTA CTTGGATAGC ATCCAACTCA GAACTTACTC CCAGTGAAAG CCTTGCTACT 
ACTGATGATG AAACTTTTGA GAAGAACTTT GAGAGAGAAA CACATAAAAT AAGTGAGCAA 
AATGATGCTG ATAATGCTAG TGTATCTTCA ACTTGTGAGC CTTTTGCAAC AGATGTTCTG 
GGCGACACTG TGATTCACTT AGATCAGGCA TTAGCCAGAA TGAGAGACTA TACGCATATG 
AAGATGGAGG CTGAAAGTAG TCCAGACGTC AGGTGCACCT GCAGGATCAT TGAGGATGAC 
GATGGTGCTG CTGCCACAAC CACGATTAGT AATAATTTAG AAGAAACTCC TGTTATTGAA 
AATCATAGTT CACAGCAACC CGTGAGTGAA GTTGCTACCG TCCCGTGTCC TAGAATTGAT 
ACTCAGCAAC TGGACCGGCA AATTAAAGCA ATTATGAAAG AAGTCATTCC TTTTTTGAAG 
GAGCACATGG ATGAAGTATG TTCTTCTCAA CTTCTAACCT CAGTAAGACG CATGGTTTTG 
GTCCTTACTC AGCAAAACAA TGAGAGCAAA GAGTTTGTAA AGTTCTTTCA TAAGCAGCTT 
GGAAGTATAT TACAGGATTC ACTCGCAAAA TTTGCTGGCA GAAAACTGAA GGATTGTGGA 
GAAGATCTTC TTGTAGAGAT ATCTGAAGTG TTATTTAATG AATTGGCTTT CTTTAAGCTC 
ATGCAAGATT TGGATAATAA TAGTGTAACT GTTAAACAGA GATGCAAAAG GAAAATAGAA 
GCTGCTGGAG TGATACAGTC TTATGCAAAA GAGGCCAAAA GGATTCTTGA AGGAGATCAC 
AGTTCACCTG CTGGAGAAAT TGATGATGAA GACAAAGACA AGGATGAGAC GGAAACAGTT 
AAGCAGACTC AAACGTCTGA GGTTTATGAT GGTGACGGCC CCAAAAATGT GAGATCTGAT 
GTTTCTGATC AAGAGGATGA TGAAGAAAGT GAAGAATGTC CAGTGTCTAT TAATTTGTCC 
AAAGCTGAAA CTCAGGCTTT GACTAATTAC GGAAGTGGAG AAGATGAGAA TGAAGATGAA 
GAAATAGAAG AATTTGAAGA GGGACCTGTA GATGTCCAGA CGTCACTTCA GGCTAACACT 
GAAACTACAG AAGAAAATGA ACACGATGAT CAGGTTCCAC AGCATGATTT TGAAAAATCA 
TCAGAAAGCA AAAATGTCCC ATCAGAACAA GAACCCACTA CTAGTAAAGA CTTTCTTTTG 
ATGACAGGTG ACCAGAATAG CACTCCTGTG AAGCCCTGTT ACCTCAGTAT CGTGGAAAAT 
GAGCAACCTT TAAATAGCCC TGTCCAGAAG GACACTCTCA CTACCATCGA CTCATCCAGT 
CAGCCTAACC CTTTGCCTTT ACCTTTAACT GAAATTGAAA CCTTGGTGCC TAGAGTCAAA 
GAAGTGAAAT CTGCTCAGGA GACTCCTGAA AGTTCTTTGG CTGGAAGTCC TGATACTGAA 
TCTCCAGTGT TAGTGAATGA CTATGAAGCA GAATCTGGTA ACATAAGTCA AAAATCTGAT 
GAAGAAGACT TTGTGAAAGT TGAAGATTTA CCACTTAAAC TGACAATATA TTCAGAGGCA 
GATCTAAGGA AGAAAATGGC CGAAGAAGAA CAGAAAAACC ACTTATCTGG TGAAATATGT 
GCAATGCAGA CTGAAGAATT AGCTGGGAAT TCTCAGACAC TAAAAGAACC TGAGCAAGTA 
TGTGCTCAGT CTGCCATCTT AAGATGGAAA CCCTTACCTG TGCCTTTTAA ACTGTTGGTG 
GTGTCATCAG GGAATTATTG TAGCAAAAAA CAGTGGGAGC CCAAAGTACA TGAGATGTCT 
CCAGAGGCTC AGCTAACTCT TTCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025135192.1
CDS	320..7006
Translation	MATGGGPFEEGVNDQDLPNWSNESVDDRLNNMDWGSQQKKANRSSEKNKRKFGVESDKRVTNDISPESSPGVGRRRTKTSLTFPHSRYMTQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDATVSPQKREMIGSAQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIREYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLQKILARENEEEDVRTIDSAVGSGSVAESTSLNIDVRSEASDATEASFSLRIRPCVEDKLGNSASQAQVSDTDVTTSPKGKGDRPPQHDRELRPNRKCSRKRGFPSKARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSEKVAETTPGHHTVPGRQPAHSPLHQRVPIRETTGSLSGVSITSELNEELNDLIQHFHNQLRDSQPPAVPDNRRQAESLSLTREISQSRNPSVSEHLPDEKVQLFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSFVPSSASPQRSVDQRSTTTAPSAPVVLTPVVNGESNNLTPSVPYPAASLVSQNQNENEGHLNPTEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENTKEEETEESEYDSEHENSEPVTNIRNPQVAATWNEVNSNSNAQCVSNNRDGRAVNSNCEINNRSAANIRALNMPPLSADCRYNREGEQEMPVAQGHEEEEEEEEEAEDEALSGASLSSHSSSLIIETPEDAEFEHKIQKLMAAKLKLRHLQNLVAMVQDDDAANQGVTSVEDFFPAEDTKQNANNTRGNANKTQKDAGVNEKAREKFYEAKLQQQQRELKQLQEERKKLIEIQEKIQELQKACPNLQLSAASVGNCPTKTHMPAVTSSPTVNENEASTNKSGFEPHDSSVVDNEVWSDMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETASPVAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGIIRTDEEEEEEEQDASSNDNFHMYPPNSANHNSYNVKETKNRWKNNRPFSADGNYRPLAKTRQQNISMQRQENLRWVSELSYVEEKEQWQEQINQLKKQLDFSVNICQTLMQDQQTLSCLLQTLLTSPYSVMPSSVASPQVHLIMHQLNQCYTQLTWQQNNVQRLKQMLSELMRQQNQHPEKPASKEGGSGTSHPPSPSLFCPFNFPTQPVNLFNLPGFTNFSSFAPSMNFSPLFPSNFGDFSQNISTPTEQQQPLAQNPSGKTEYMAFPKPFESSSSIGAEKQRNQKQPEEEVENSRTPWFYDQEGEVEKPFLKTGFAVSVEKTTNSNRKNQLDTSRRRRQFDEVSLESFNSMLDPVDPTTVTKTFKSRKASAQASLASKDKTPKSKSKKRHSTQLKSRVKNIGYESASVSSTCEPCKSRNRHSAQTEEPVQAKVLSRKNHEQLEKVIRCSRSTEISSAHARRILQQSNRNACNEAPETGSDFSMFEALRDTIYSEVATLISQNESRPHFLIELFHELQLLNTDYLRQRALYALQDIVSRHISENHEKEGENIKSVNSGTWIASNSELTPSESLATTDDETFEKNFERETHKISEQNDADNASVSSTCEPFATDVLGDTVIHLDQALARMRDYTHMKMEAESSPDVRCTCRIIEDDDGAAATTTISNNLEETPVIENHSSQQPVSEVATVPCPRIDTQQLDRQIKAIMKEVIPFLKEHMDEVCSSQLLTSVRRMVLVLTQQNNESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSVTVKQRCKRKIEAAGVIQSYAKEAKRILEGDHSSPAGEIDDEDKDKDETETVKQTQTSEVYDGDGPKNVRSDVSDQEDDEESEECPVSINLSKAETQALTNYGSGEDENEDEEIEEFEEGPVDVQTSLQANTETTEENEHDDQVPQHDFEKSSESKNVPSEQEPTTSKDFLLMTGDQNSTPVKPCYLSIVENEQPLNSPVQKDTLTTIDSSSQPNPLPLPLTEIETLVPRVKEVKSAQETPESSLAGSPDTESPVLVNDYEAESGNISQKSDEEDFVKVEDLPLKLTIYSEADLRKKMAEEEQKNHLSGEICAMQTEELAGNSQTLKEPEQVCAQSAILRWKPLPVPFKLLVVSSGNYCSKKQWEPKVHEMSPEAQLTLS

Target ORF information:

RefSeq Version	XM_025279407.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis pericentriolar material 1 (PCM1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025279407.1

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
AGCAATGAGA GTGTTGATGA CCGACTCAAC AATATGGATT GGGGCAGCCA GCAGAAGAAG 
GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA 
ACTAACGATA TTTCTCCGGA GTCATCACCG GGAGTTGGAA GACGAAGAAC AAAGACTTCA 
CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TCCCCGAGCA GGCAGAACTA 
GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCTCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCCAC TGTAAGTCCT 
CAAAAGAGAG AAATGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAGATTCGTG AGTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGACCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTCACT 
CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT CCAGAAGATT 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCGGT CTGAGGCTTC AGATGCCACG 
GAGGCATCTT TTAGTCTGAG AATTCGGCCG TGCGTTGAGG ACAAACTAGG GAATTCAGCT 
TCACAAGCAC AGGTTTCAGA CACTGACGTT ACTACAAGTC CAAAAGGGAA AGGTGACAGA 
CCTCCTCAGC ATGACAGGGA GCTGAGGCCT AATAGGAAAT GTAGCCGAAA ACGTGGATTT 
CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATGGAAG AGATAGAAAA TTTGAAGAAA 
CAACATGATT TATTAAAAAG GATGTTACAG CAGCAGGAGC AGCTGAGAGC TCTACAAGGA 
CGACAAGCTG CTCTGCTTGC CCTGCAGCAT AAAGCTGAGC AGGCTATAGC TGTGATGGAT 
GATTCTGAAA AGGTTGCGGA GACAACTCCT GGCCATCATA CTGTACCAGG CAGACAGCCG 
GCTCATTCAC CTTTGCATCA GAGAGTACCC ATAAGAGAAA CTACAGGTAG CCTATCTGGA 
GTCAGTATCA CCTCTGAACT AAACGAAGAA TTGAATGATT TAATTCAGCA TTTTCATAAT 
CAGCTTCGTG ATTCTCAGCC TCCAGCTGTT CCAGACAATA GAAGACAAGC AGAAAGTCTT 
TCATTAACTA GGGAGATTTC TCAGAGCAGA AATCCTTCAG TTTCAGAGCA TTTACCTGAT 
GAGAAAGTCC AACTTTTTAG CAAGATGAGA GTGTTACAAG AAAAGAAACA AAAAATGGAC 
AAACTTCTTG GAGAACTTCA TACACTTCGA GATCAGCATT TGAACAATTC ATCATTTGTG 
CCTTCTTCAG CCTCTCCACA AAGGAGTGTG GATCAGAGAA GTACAACCAC AGCTCCTTCT 
GCTCCTGTAG TCTTAACACC AGTGGTCAAT GGAGAATCCA ATAACCTCAC GCCATCTGTT 
CCTTATCCTG CTGCTTCTCT AGTTTCTCAG AATCAGAATG AAAATGAAGG CCACCTAAAT 
CCAACTGAAA AACTCCAGAA GTTAAATGAA GTTCGAAAGA GGTTGAATGA ACTAAGAGAA 
TTAGTTCATT ATTATGAGCA AACATCAGAT ATGATGACAG ATGCTGTGAA TGAAAACACA 
AAAGAAGAAG AAACTGAAGA ATCAGAATAT GATTCGGAGC ATGAAAACTC GGAACCTGTT 
ACTAACATTC GAAATCCACA AGTGGCTGCC ACCTGGAACG AAGTAAATAG TAATTCTAAT 
GCACAGTGTG TTTCCAACAA TAGAGATGGG CGAGCAGTTA ATTCTAACTG TGAAATTAAC 
AACAGGTCTG CTGCCAACAT AAGGGCTCTA AACATGCCTC CTCTTTCAGC AGATTGTCGA 
TACAATAGAG AAGGAGAACA GGAGATGCCT GTTGCACAAG GTCATGAGGA GGAGGAGGAG 
GAGGAGGAGG AAGCAGAAGA TGAGGCCCTC AGTGGAGCTT CACTGTCTAG TCACAGCAGC 
AGCCTGATCA TTGAGACTCC TGAAGATGCT GAATTTGAAC ATAAGATCCA GAAGCTTATG 
GCTGCAAAAC TGAAACTTAG ACACTTACAG AACCTTGTAG CCATGGTGCA GGACGATGAT 
GCAGCCAATC AAGGAGTTAC TTCTGTGGAG GATTTCTTCC CAGCAGAAGA CACCAAACAA 
AATGCAAATA ACACTAGAGG AAATGCCAAT AAAACACAGA AAGATGCTGG AGTAAATGAA 
AAGGCAAGGG AGAAATTTTA TGAGGCTAAA CTACAGCAGC AACAGAGAGA ACTCAAACAA 
TTGCAGGAAG AAAGAAAGAA ACTGATTGAG ATTCAAGAGA AAATTCAAGA ATTGCAAAAG 
GCATGTCCTA ACCTACAGCT GTCAGCTGCT AGTGTGGGTA ATTGTCCCAC AAAAACACAT 
ATGCCAGCTG TTACTTCATC CCCAACCGTT AATGAAAATG AAGCCAGTAC AAACAAATCT 
GGTTTTGAGC CTCATGATTC TTCAGTGGTA GATAATGAGG TATGGTCAGA TATGCGAAGA 
CATGAAATGT TAAGGGAGGA GCTACGACAG AGAAGAAAGC AGCTGGAAGC TCTGATGGCT 
GAACATCAGA GGAGGCAAGG TCTGGCTGAA ACTGCATCTC CAGTGGCTGT TTCACTGAGA 
AGTGATGGAT CTGAGAACCT GTGTACACCT CAGCAAAGCA GAACAGAAAA AACAATGGCA 
ACTTGGGGAG GTTCTACCCA GTGTGCCCTA GATGAAGAAG GAGATGAAGA TGGTTACCTT 
TCTGAAGGAA TTATTCGGAC AGACGAAGAG GAGGAAGAGG AGGAGCAAGA TGCCAGTTCC 
AATGATAACT TTCATATGTA TCCTCCAAAC AGTGCAAATC ATAATTCTTA CAATGTAAAG 
GAAACTAAAA ATAGGTGGAA GAACAATCGC CCTTTTTCAG CAGATGGAAA TTATCGTCCT 
TTAGCCAAGA CAAGACAGCA GAATATCAGC ATGCAGCGTC AGGAAAACCT TCGCTGGGTG 
TCCGAACTCT CTTATGTAGA AGAGAAAGAA CAATGGCAAG AACAAATTAA TCAGCTAAAG 
AAACAACTTG ATTTCAGTGT CAATATTTGT CAGACTTTAA TGCAAGACCA GCAGACTCTG 
TCTTGTCTGC TACAAACTCT TCTCACGAGT CCTTATAGTG TTATGCCCAG CAGTGTTGCA 
TCCCCGCAAG TGCACCTTAT AATGCACCAG TTGAATCAGT GCTATACCCA GCTAACGTGG 
CAGCAGAACA ATGTCCAGAG GTTGAAACAA ATGCTCAGTG AACTCATGCG CCAACAAAAT 
CAGCATCCAG AAAAACCTGC AAGCAAGGAA GGAGGCAGTG GTACATCACA CCCTCCTTCT 
CCCAGTTTAT TTTGTCCTTT CAACTTTCCA ACTCAGCCTG TAAATCTGTT TAACCTACCG 
GGGTTTACTA ATTTTTCGTC ATTTGCACCA AGTATGAATT TCAGCCCTTT ATTTCCTTCT 
AATTTTGGAG ATTTTTCTCA AAATATTTCT ACACCCACTG AACAGCAGCA ACCGTTAGCC 
CAGAATCCTT CAGGGAAAAC GGAATACATG GCTTTTCCAA AACCTTTTGA AAGTAGTTCT 
TCTATTGGAG CGGAAAAACA AAGGAATCAA AAACAGCCTG AAGAGGAGGT GGAGAACAGT 
AGGACACCAT GGTTTTATGA CCAAGAAGGT GAAGTAGAAA AGCCATTTCT CAAGACTGGA 
TTTGCAGTGT CTGTAGAGAA AACAACAAAT AGTAATCGCA AAAATCAACT AGATACCAGT 
AGGAGAAGAC GCCAGTTTGA TGAAGTGTCC TTAGAAAGCT TTAACAGTAT GCTTGACCCA 
GTAGATCCAA CAACAGTAAC TAAAACATTC AAGTCAAGAA AAGCATCTGC ACAGGCCAGC 
CTGGCATCTA AAGATAAAAC TCCCAAATCA AAGAGTAAGA AGAGGCATTC TACTCAGCTG 
AAAAGCAGAG TGAAAAATAT CGGGTATGAA AGTGCCAGTG TGTCTAGCAC ATGTGAACCT 
TGCAAAAGTA GAAACAGACA TTCAGCCCAA ACTGAAGAGC CTGTTCAAGC AAAAGTACTC 
AGCAGAAAGA ATCATGAGCA GCTGGAAAAA GTCATAAGAT GTAGTAGGTC TACAGAAATA 
TCTTCAGCAC ATGCTAGGAG AATACTACAG CAGTCTAACA GAAATGCATG CAATGAAGCG 
CCAGAAACCG GGAGTGATTT TTCCATGTTC GAAGCTTTGC GGGATACCAT TTATTCTGAA 
GTAGCTACGT TGATTTCTCA AAATGAATCT CGTCCACATT TTCTTATTGA ACTCTTCCAT 
GAGCTTCAGC TACTTAATAC AGACTATTTG AGACAGAGGG CCTTATATGC ATTGCAGGAC 
ATAGTATCCA GGCATATTTC TGAGAACCAT GAAAAAGAAG GAGAAAATAT AAAGTCCGTG 
AATTCTGGTA CTTGGATAGC ATCCAACTCA GAACTTACTC CCAGTGAAAG CCTTGCTACT 
ACTGATGATG AAACTTTTGA GAAGAACTTT GAGAGAGAAA CACATAAAAT AAGTGAGCAA 
AATGATGCTG ATAATGCTAG TGTATCTTCA ACTTGTGAGC CTTTTGCAAC AGATGTTCTG 
GGCGACACTG TGATTCACTT AGATCAGGCA TTAGCCAGAA TGAGAGACTA TACGCATATG 
AAGATGGAGG CTGAAAGTAG TCCAGACGTC AGGTGCACCT GCAGGATCAT TGAGGATGAC 
GATGGTGCTG CTGCCACAAC CACGATTAGT AATAATTTAG AAGAAACTCC TGTTATTGAA 
AATCATAGTT CACAGCAACC CGTGAGTGAA GTTGCTACCG TCCCGTGTCC TAGAATTGAT 
ACTCAGCAAC TGGACCGGCA AATTAAAGCA ATTATGAAAG AAGTCATTCC TTTTTTGAAG 
GAGCACATGG ATGAAGTATG TTCTTCTCAA CTTCTAACCT CAGTAAGACG CATGGTTTTG 
GTCCTTACTC AGCAAAACAA TGAGAGCAAA GAGTTTGTAA AGTTCTTTCA TAAGCAGCTT 
GGAAGTATAT TACAGGATTC ACTCGCAAAA TTTGCTGGCA GAAAACTGAA GGATTGTGGA 
GAAGATCTTC TTGTAGAGAT ATCTGAAGTG TTATTTAATG AATTGGCTTT CTTTAAGCTC 
ATGCAAGATT TGGATAATAA TAGTGTAACT GTTAAACAGA GATGCAAAAG GAAAATAGAA 
GCTGCTGGAG TGATACAGTC TTATGCAAAA GAGGCCAAAA GGATTCTTGA AGGAGATCAC 
AGTTCACCTG CTGGAGAAAT TGATGATGAA GACAAAGACA AGGATGAGAC GGAAACAGTT 
AAGCAGACTC AAACGTCTGA GGTTTATGAT GGTGACGGCC CCAAAAATGT GAGATCTGAT 
GTTTCTGATC AAGAGGATGA TGAAGAAAGT GAAGAATGTC CAGTGTCTAT TAATTTGTCC 
AAAGCTGAAA CTCAGGCTTT GACTAATTAC GGAAGTGGAG AAGATGAGAA TGAAGATGAA 
GAAATAGAAG AATTTGAAGA GGGACCTGTA GATGTCCAGA CGTCACTTCA GGCTAACACT 
GAAACTACAG AAGAAAATGA ACACGATGAT CAGGTTCCAC AGCATGATTT TGAAAAATCA 
TCAGAAAGCA AAAATGTCCC ATCAGAACAA GAACCCACTA CTAGTAAAGA CTTTCTTTTG 
ATGACAGGTG ACCAGAATAG CACTCCTGTG AAGCCCTGTT ACCTCAGTAT CGTGGAAAAT 
GAGCAACCTT TAAATAGCCC TGTCCAGAAG GACACTCTCA CTACCATCGA CTCATCCAGT 
CAGCCTAACC CTTTGCCTTT ACCTTTAACT GAAATTGAAA CCTTGGTGCC TAGAGTCAAA 
GAAGTGAAAT CTGCTCAGGA GACTCCTGAA AGTTCTTTGG CTGGAAGTCC TGATACTGAA 
TCTCCAGTGT TAGTGAATGA CTATGAAGCA GAATCTGGTA ACATAAGTCA AAAATCTGAT 
GAAGAAGACT TTGTGAAAGT TGAAGATTTA CCACTTAAAC TGACAATATA TTCAGAGGCA 
GATCTAAGGA AGAAAATGGC CGAAGAAGAA CAGAAAAACC ACTTATCTGG TGAAATATGT 
GCAATGCAGA CTGAAGAATT AGCTGGGAAT TCTCAGACAC TAAAAGAACC TGAGCAAGTA 
TGTGCTCAGT CTGCCATCTT AAGATGGAAA CCCTTACCTG TGCCTTTTAA ACTGTTGGTG 
GTGTCATCAG GGAATTATTG TAGCAAAAAA CAGTGGGAGC CCAAAGTACA TGAGATGTCT 
CCAGAGGCTC AGCTAACTCT TTCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( XM_025279414.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025135199.1 Bubalus bubalis pericentriolar material 1 (PCM1), transcript variant X3, mRNA.

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CloneID	OBu42291
Clone ID Related Accession (Same CDS sequence)	XM_025279414.1
Accession Version	XM_025279414.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6684bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	pericentriolar material 1 protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037545.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG GAGGAGGTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
AGCAATGAGA GTGTTGATGA CCGACTCAAC AATATGGATT GGGGCAGCCA GCAGAAGAAG 
GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA 
ACTAACGATA TTTCTCCGGA GTCATCACCG GGAGTTGGAA GACGAAGAAC AAAGACTTCA 
CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TCCCCGAGCA GGCAGAACTA 
GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCTCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCCAC TGTAAGTCCT 
CAAAAGAGAG AAATGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAGATTCGTG AGTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGACCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTCACT 
CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT CCAGAAGATT 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCGGT CTGAGGCTTC AGATGCCACG 
GAGGCATCTT TTAGTCTGAG AATTCGGCCG TGCGTTGAGG ACAAACTAGG GAATTCAGCT 
TCACAAGCAC AGGTTTCAGA CACTGACGTT ACTACAAGTC CAAAAGGGAA AGGTGACAGA 
CCTCCTCAGC ATGACAGGGA GCTGAGGCCT AATAGGAAAT GTAGCCGAAA ACGTGGATTT 
CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATGGAAG AGATAGAAAA TTTGAAGAAA 
CAACATGATT TATTAAAAAG GATGTTACAG CAGCAGGAGC AGCTGAGAGC TCTACAAGGA 
CGACAAGCTG CTCTGCTTGC CCTGCAGCAT AAAGCTGAGC AGGCTATAGC TGTGATGGAT 
GATTCTGAAA AGGTTGCGGA GACAACTCCT GGCCATCATA CTGTACCAGG CAGACAGCCG 
GCTCATTCAC CTTTGCATCA GAGAGTACCC ATAAGAGAAA CTACAGGTAG CCTATCTGGA 
GTCAGTATCA CCTCTGAACT AAACGAAGAA TTGAATGATT TAATTCAGCA TTTTCATAAT 
CAGCTTCGTG ATTCTCAGCC TCCAGCTGTT CCAGACAATA GAAGACAAGC AGAAAGTCTT 
TCATTAACTA GGGAGATTTC TCAGAGCAGA AATCCTTCAG TTTCAGAGCA TTTACCTGAT 
GAGAAAGTCC AACTTTTTAG CAAGATGAGA GTGTTACAAG AAAAGAAACA AAAAATGGAC 
AAACTTCTTG GAGAACTTCA TACACTTCGA GATCAGCATT TGAACAATTC ATCATTTGTG 
CCTTCTTCAG CCTCTCCACA AAGGAGTGTG GATCAGAGAA GTACAACCAC AGCTCCTTCT 
GCTCCTGTAG TCTTAACACC AGTGGTCAAT GGAGAATCCA ATAACCTCAC GCCATCTGTT 
CCTTATCCTG CTGCTTCTCT AGTTTCTCAG AATCAGAATG AAAATGAAGG CCACCTAAAT 
CCAACTGAAA AACTCCAGAA GTTAAATGAA GTTCGAAAGA GGTTGAATGA ACTAAGAGAA 
TTAGTTCATT ATTATGAGCA AACATCAGAT ATGATGACAG ATGCTGTGAA TGAAAACACA 
AAAGAAGAAG AAACTGAAGA ATCAGAATAT GATTCGGAGC ATGAAAACTC GGAACCTGTT 
ACTAACATTC GAAATCCACA AGTGGCTGCC ACCTGGAACG AAGTAAATAG TAATTCTAAT 
GCACAGTGTG TTTCCAACAA TAGAGATGGG CGAGCAGTTA ATTCTAACTG TGAAATTAAC 
AACAGGTCTG CTGCCAACAT AAGGGCTCTA AACATGCCTC CTCTTTCAGA TTGTCGATAC 
AATAGAGAAG GAGAACAGGA GATGCCTGTT GCACAAGGTC ATGAGGAGGA GGAGGAGGAG 
GAGGAGGAAG CAGAAGATGA GGCCCTCAGT GGAGCTTCAC TGTCTAGTCA CAGCAGCAGC 
CTGATCATTG AGACTCCTGA AGATGCTGAA TTTGAACATA AGATCCAGAA GCTTATGGCT 
GCAAAACTGA AACTTAGACA CTTACAGAAC CTTGTAGCCA TGGTGCAGGA CGATGATGCA 
GCCAATCAAG GAGTTACTTC TGTGGAGGAT TTCTTCCCAG CAGAAGACAC CAAACAAAAT 
GCAAATAACA CTAGAGGAAA TGCCAATAAA ACACAGAAAG ATGCTGGAGT AAATGAAAAG 
GCAAGGGAGA AATTTTATGA GGCTAAACTA CAGCAGCAAC AGAGAGAACT CAAACAATTG 
CAGGAAGAAA GAAAGAAACT GATTGAGATT CAAGAGAAAA TTCAAGAATT GCAAAAGGCA 
TGTCCTAACC TACAGCTGTC AGCTGCTAGT GTGGGTAATT GTCCCACAAA AACACATATG 
CCAGCTGTTA CTTCATCCCC AACCGTTAAT GAAAATGAAG CCAGTACAAA CAAATCTGGT 
TTTGAGCCTC ATGATTCTTC AGTGGTAGAT AATGAGGTAT GGTCAGATAT GCGAAGACAT 
GAAATGTTAA GGGAGGAGCT ACGACAGAGA AGAAAGCAGC TGGAAGCTCT GATGGCTGAA 
CATCAGAGGA GGCAAGGTCT GGCTGAAACT GCATCTCCAG TGGCTGTTTC ACTGAGAAGT 
GATGGATCTG AGAACCTGTG TACACCTCAG CAAAGCAGAA CAGAAAAAAC AATGGCAACT 
TGGGGAGGTT CTACCCAGTG TGCCCTAGAT GAAGAAGGAG ATGAAGATGG TTACCTTTCT 
GAAGGAATTA TTCGGACAGA CGAAGAGGAG GAAGAGGAGG AGCAAGATGC CAGTTCCAAT 
GATAACTTTC ATATGTATCC TCCAAACAGT GCAAATCATA ATTCTTACAA TGTAAAGGAA 
ACTAAAAATA GGTGGAAGAA CAATCGCCCT TTTTCAGCAG ATGGAAATTA TCGTCCTTTA 
GCCAAGACAA GACAGCAGAA TATCAGCATG CAGCGTCAGG AAAACCTTCG CTGGGTGTCC 
GAACTCTCTT ATGTAGAAGA GAAAGAACAA TGGCAAGAAC AAATTAATCA GCTAAAGAAA 
CAACTTGATT TCAGTGTCAA TATTTGTCAG ACTTTAATGC AAGACCAGCA GACTCTGTCT 
TGTCTGCTAC AAACTCTTCT CACGAGTCCT TATAGTGTTA TGCCCAGCAG TGTTGCATCC 
CCGCAAGTGC ACCTTATAAT GCACCAGTTG AATCAGTGCT ATACCCAGCT AACGTGGCAG 
CAGAACAATG TCCAGAGGTT GAAACAAATG CTCAGTGAAC TCATGCGCCA ACAAAATCAG 
CATCCAGAAA AACCTGCAAG CAAGGAAGGA GGCAGTGGTA CATCACACCC TCCTTCTCCC 
AGTTTATTTT GTCCTTTCAA CTTTCCAACT CAGCCTGTAA ATCTGTTTAA CCTACCGGGG 
TTTACTAATT TTTCGTCATT TGCACCAAGT ATGAATTTCA GCCCTTTATT TCCTTCTAAT 
TTTGGAGATT TTTCTCAAAA TATTTCTACA CCCACTGAAC AGCAGCAACC GTTAGCCCAG 
AATCCTTCAG GGAAAACGGA ATACATGGCT TTTCCAAAAC CTTTTGAAAG TAGTTCTTCT 
ATTGGAGCGG AAAAACAAAG GAATCAAAAA CAGCCTGAAG AGGAGGTGGA GAACAGTAGG 
ACACCATGGT TTTATGACCA AGAAGGTGAA GTAGAAAAGC CATTTCTCAA GACTGGATTT 
GCAGTGTCTG TAGAGAAAAC AACAAATAGT AATCGCAAAA ATCAACTAGA TACCAGTAGG 
AGAAGACGCC AGTTTGATGA AGTGTCCTTA GAAAGCTTTA ACAGTATGCT TGACCCAGTA 
GATCCAACAA CAGTAACTAA AACATTCAAG TCAAGAAAAG CATCTGCACA GGCCAGCCTG 
GCATCTAAAG ATAAAACTCC CAAATCAAAG AGTAAGAAGA GGCATTCTAC TCAGCTGAAA 
AGCAGAGTGA AAAATATCGG GTATGAAAGT GCCAGTGTGT CTAGCACATG TGAACCTTGC 
AAAAGTAGAA ACAGACATTC AGCCCAAACT GAAGAGCCTG TTCAAGCAAA AGTACTCAGC 
AGAAAGAATC ATGAGCAGCT GGAAAAAGTC ATAAGATGTA GTAGGTCTAC AGAAATATCT 
TCAGCACATG CTAGGAGAAT ACTACAGCAG TCTAACAGAA ATGCATGCAA TGAAGCGCCA 
GAAACCGGGA GTGATTTTTC CATGTTCGAA GCTTTGCGGG ATACCATTTA TTCTGAAGTA 
GCTACGTTGA TTTCTCAAAA TGAATCTCGT CCACATTTTC TTATTGAACT CTTCCATGAG 
CTTCAGCTAC TTAATACAGA CTATTTGAGA CAGAGGGCCT TATATGCATT GCAGGACATA 
GTATCCAGGC ATATTTCTGA GAACCATGAA AAAGAAGGAG AAAATATAAA GTCCGTGAAT 
TCTGGTACTT GGATAGCATC CAACTCAGAA CTTACTCCCA GTGAAAGCCT TGCTACTACT 
GATGATGAAA CTTTTGAGAA GAACTTTGAG AGAGAAACAC ATAAAATAAG TGAGCAAAAT 
GATGCTGATA ATGCTAGTGT ATCTTCAACT TGTGAGCCTT TTGCAACAGA TGTTCTGGGC 
GACACTGTGA TTCACTTAGA TCAGGCATTA GCCAGAATGA GAGACTATAC GCATATGAAG 
ATGGAGGCTG AAAGTAGTCC AGACGTCAGG TGCACCTGCA GGATCATTGA GGATGACGAT 
GGTGCTGCTG CCACAACCAC GATTAGTAAT AATTTAGAAG AAACTCCTGT TATTGAAAAT 
CATAGTTCAC AGCAACCCGT GAGTGAAGTT GCTACCGTCC CGTGTCCTAG AATTGATACT 
CAGCAACTGG ACCGGCAAAT TAAAGCAATT ATGAAAGAAG TCATTCCTTT TTTGAAGGAG 
CACATGGATG AAGTATGTTC TTCTCAACTT CTAACCTCAG TAAGACGCAT GGTTTTGGTC 
CTTACTCAGC AAAACAATGA GAGCAAAGAG TTTGTAAAGT TCTTTCATAA GCAGCTTGGA 
AGTATATTAC AGGATTCACT CGCAAAATTT GCTGGCAGAA AACTGAAGGA TTGTGGAGAA 
GATCTTCTTG TAGAGATATC TGAAGTGTTA TTTAATGAAT TGGCTTTCTT TAAGCTCATG 
CAAGATTTGG ATAATAATAG TGTAACTGTT AAACAGAGAT GCAAAAGGAA AATAGAAGCT 
GCTGGAGTGA TACAGTCTTA TGCAAAAGAG GCCAAAAGGA TTCTTGAAGG AGATCACAGT 
TCACCTGCTG GAGAAATTGA TGATGAAGAC AAAGACAAGG ATGAGACGGA AACAGTTAAG 
CAGACTCAAA CGTCTGAGGT TTATGATGGT GACGGCCCCA AAAATGTGAG ATCTGATGTT 
TCTGATCAAG AGGATGATGA AGAAAGTGAA GAATGTCCAG TGTCTATTAA TTTGTCCAAA 
GCTGAAACTC AGGCTTTGAC TAATTACGGA AGTGGAGAAG ATGAGAATGA AGATGAAGAA 
ATAGAAGAAT TTGAAGAGGG ACCTGTAGAT GTCCAGACGT CACTTCAGGC TAACACTGAA 
ACTACAGAAG AAAATGAACA CGATGATCAG GTTCCACAGC ATGATTTTGA AAAATCATCA 
GAAAGCAAAA ATGTCCCATC AGAACAAGAA CCCACTACTA GTAAAGACTT TCTTTTGATG 
ACAGGTGACC AGAATAGCAC TCCTGTGAAG CCCTGTTACC TCAGTATCGT GGAAAATGAG 
CAACCTTTAA ATAGCCCTGT CCAGAAGGAC ACTCTCACTA CCATCGACTC ATCCAGTCAG 
CCTAACCCTT TGCCTTTACC TTTAACTGAA ATTGAAACCT TGGTGCCTAG AGTCAAAGAA 
GTGAAATCTG CTCAGGAGAC TCCTGAAAGT TCTTTGGCTG GAAGTCCTGA TACTGAATCT 
CCAGTGTTAG TGAATGACTA TGAAGCAGAA TCTGGTAACA TAAGTCAAAA ATCTGATGAA 
GAAGACTTTG TGAAAGTTGA AGATTTACCA CTTAAACTGA CAATATATTC AGAGGCAGAT 
CTAAGGAAGA AAATGGCCGA AGAAGAACAG AAAAACCACT TATCTGGTGA AATATGTGCA 
ATGCAGACTG AAGAATTAGC TGGGAATTCT CAGACACTAA AAGAACCTGA GCAAGTATGT 
GCTCAGTCTG CCATCTTAAG ATGGAAACCC TTACCTGTGC CTTTTAAACT GTTGGTGGTG 
TCATCAGGGA ATTATTGTAG CAAAAAACAG TGGGAGCCCA AAGTACATGA GATGTCTCCA 
GAGGCTCAGC TAACTCTTTC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025135199.1
CDS	392..7075
Translation	MATGGGPFEEGVNDQDLPNWSNESVDDRLNNMDWGSQQKKANRSSEKNKRKFGVESDKRVTNDISPESSPGVGRRRTKTSLTFPHSRYMTQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDATVSPQKREMIGSAQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIREYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLQKILARENEEEDVRTIDSAVGSGSVAESTSLNIDVRSEASDATEASFSLRIRPCVEDKLGNSASQAQVSDTDVTTSPKGKGDRPPQHDRELRPNRKCSRKRGFPSKARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSEKVAETTPGHHTVPGRQPAHSPLHQRVPIRETTGSLSGVSITSELNEELNDLIQHFHNQLRDSQPPAVPDNRRQAESLSLTREISQSRNPSVSEHLPDEKVQLFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSFVPSSASPQRSVDQRSTTTAPSAPVVLTPVVNGESNNLTPSVPYPAASLVSQNQNENEGHLNPTEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENTKEEETEESEYDSEHENSEPVTNIRNPQVAATWNEVNSNSNAQCVSNNRDGRAVNSNCEINNRSAANIRALNMPPLSDCRYNREGEQEMPVAQGHEEEEEEEEEAEDEALSGASLSSHSSSLIIETPEDAEFEHKIQKLMAAKLKLRHLQNLVAMVQDDDAANQGVTSVEDFFPAEDTKQNANNTRGNANKTQKDAGVNEKAREKFYEAKLQQQQRELKQLQEERKKLIEIQEKIQELQKACPNLQLSAASVGNCPTKTHMPAVTSSPTVNENEASTNKSGFEPHDSSVVDNEVWSDMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETASPVAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGIIRTDEEEEEEEQDASSNDNFHMYPPNSANHNSYNVKETKNRWKNNRPFSADGNYRPLAKTRQQNISMQRQENLRWVSELSYVEEKEQWQEQINQLKKQLDFSVNICQTLMQDQQTLSCLLQTLLTSPYSVMPSSVASPQVHLIMHQLNQCYTQLTWQQNNVQRLKQMLSELMRQQNQHPEKPASKEGGSGTSHPPSPSLFCPFNFPTQPVNLFNLPGFTNFSSFAPSMNFSPLFPSNFGDFSQNISTPTEQQQPLAQNPSGKTEYMAFPKPFESSSSIGAEKQRNQKQPEEEVENSRTPWFYDQEGEVEKPFLKTGFAVSVEKTTNSNRKNQLDTSRRRRQFDEVSLESFNSMLDPVDPTTVTKTFKSRKASAQASLASKDKTPKSKSKKRHSTQLKSRVKNIGYESASVSSTCEPCKSRNRHSAQTEEPVQAKVLSRKNHEQLEKVIRCSRSTEISSAHARRILQQSNRNACNEAPETGSDFSMFEALRDTIYSEVATLISQNESRPHFLIELFHELQLLNTDYLRQRALYALQDIVSRHISENHEKEGENIKSVNSGTWIASNSELTPSESLATTDDETFEKNFERETHKISEQNDADNASVSSTCEPFATDVLGDTVIHLDQALARMRDYTHMKMEAESSPDVRCTCRIIEDDDGAAATTTISNNLEETPVIENHSSQQPVSEVATVPCPRIDTQQLDRQIKAIMKEVIPFLKEHMDEVCSSQLLTSVRRMVLVLTQQNNESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSVTVKQRCKRKIEAAGVIQSYAKEAKRILEGDHSSPAGEIDDEDKDKDETETVKQTQTSEVYDGDGPKNVRSDVSDQEDDEESEECPVSINLSKAETQALTNYGSGEDENEDEEIEEFEEGPVDVQTSLQANTETTEENEHDDQVPQHDFEKSSESKNVPSEQEPTTSKDFLLMTGDQNSTPVKPCYLSIVENEQPLNSPVQKDTLTTIDSSSQPNPLPLPLTEIETLVPRVKEVKSAQETPESSLAGSPDTESPVLVNDYEAESGNISQKSDEEDFVKVEDLPLKLTIYSEADLRKKMAEEEQKNHLSGEICAMQTEELAGNSQTLKEPEQVCAQSAILRWKPLPVPFKLLVVSSGNYCSKKQWEPKVHEMSPEAQLTLS

Target ORF information:

RefSeq Version	XM_025279414.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis pericentriolar material 1 (PCM1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025279414.1

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
AGCAATGAGA GTGTTGATGA CCGACTCAAC AATATGGATT GGGGCAGCCA GCAGAAGAAG 
GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA 
ACTAACGATA TTTCTCCGGA GTCATCACCG GGAGTTGGAA GACGAAGAAC AAAGACTTCA 
CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TCCCCGAGCA GGCAGAACTA 
GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCTCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCCAC TGTAAGTCCT 
CAAAAGAGAG AAATGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAGATTCGTG AGTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGACCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTCACT 
CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT CCAGAAGATT 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCGGT CTGAGGCTTC AGATGCCACG 
GAGGCATCTT TTAGTCTGAG AATTCGGCCG TGCGTTGAGG ACAAACTAGG GAATTCAGCT 
TCACAAGCAC AGGTTTCAGA CACTGACGTT ACTACAAGTC CAAAAGGGAA AGGTGACAGA 
CCTCCTCAGC ATGACAGGGA GCTGAGGCCT AATAGGAAAT GTAGCCGAAA ACGTGGATTT 
CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATGGAAG AGATAGAAAA TTTGAAGAAA 
CAACATGATT TATTAAAAAG GATGTTACAG CAGCAGGAGC AGCTGAGAGC TCTACAAGGA 
CGACAAGCTG CTCTGCTTGC CCTGCAGCAT AAAGCTGAGC AGGCTATAGC TGTGATGGAT 
GATTCTGAAA AGGTTGCGGA GACAACTCCT GGCCATCATA CTGTACCAGG CAGACAGCCG 
GCTCATTCAC CTTTGCATCA GAGAGTACCC ATAAGAGAAA CTACAGGTAG CCTATCTGGA 
GTCAGTATCA CCTCTGAACT AAACGAAGAA TTGAATGATT TAATTCAGCA TTTTCATAAT 
CAGCTTCGTG ATTCTCAGCC TCCAGCTGTT CCAGACAATA GAAGACAAGC AGAAAGTCTT 
TCATTAACTA GGGAGATTTC TCAGAGCAGA AATCCTTCAG TTTCAGAGCA TTTACCTGAT 
GAGAAAGTCC AACTTTTTAG CAAGATGAGA GTGTTACAAG AAAAGAAACA AAAAATGGAC 
AAACTTCTTG GAGAACTTCA TACACTTCGA GATCAGCATT TGAACAATTC ATCATTTGTG 
CCTTCTTCAG CCTCTCCACA AAGGAGTGTG GATCAGAGAA GTACAACCAC AGCTCCTTCT 
GCTCCTGTAG TCTTAACACC AGTGGTCAAT GGAGAATCCA ATAACCTCAC GCCATCTGTT 
CCTTATCCTG CTGCTTCTCT AGTTTCTCAG AATCAGAATG AAAATGAAGG CCACCTAAAT 
CCAACTGAAA AACTCCAGAA GTTAAATGAA GTTCGAAAGA GGTTGAATGA ACTAAGAGAA 
TTAGTTCATT ATTATGAGCA AACATCAGAT ATGATGACAG ATGCTGTGAA TGAAAACACA 
AAAGAAGAAG AAACTGAAGA ATCAGAATAT GATTCGGAGC ATGAAAACTC GGAACCTGTT 
ACTAACATTC GAAATCCACA AGTGGCTGCC ACCTGGAACG AAGTAAATAG TAATTCTAAT 
GCACAGTGTG TTTCCAACAA TAGAGATGGG CGAGCAGTTA ATTCTAACTG TGAAATTAAC 
AACAGGTCTG CTGCCAACAT AAGGGCTCTA AACATGCCTC CTCTTTCAGA TTGTCGATAC 
AATAGAGAAG GAGAACAGGA GATGCCTGTT GCACAAGGTC ATGAGGAGGA GGAGGAGGAG 
GAGGAGGAAG CAGAAGATGA GGCCCTCAGT GGAGCTTCAC TGTCTAGTCA CAGCAGCAGC 
CTGATCATTG AGACTCCTGA AGATGCTGAA TTTGAACATA AGATCCAGAA GCTTATGGCT 
GCAAAACTGA AACTTAGACA CTTACAGAAC CTTGTAGCCA TGGTGCAGGA CGATGATGCA 
GCCAATCAAG GAGTTACTTC TGTGGAGGAT TTCTTCCCAG CAGAAGACAC CAAACAAAAT 
GCAAATAACA CTAGAGGAAA TGCCAATAAA ACACAGAAAG ATGCTGGAGT AAATGAAAAG 
GCAAGGGAGA AATTTTATGA GGCTAAACTA CAGCAGCAAC AGAGAGAACT CAAACAATTG 
CAGGAAGAAA GAAAGAAACT GATTGAGATT CAAGAGAAAA TTCAAGAATT GCAAAAGGCA 
TGTCCTAACC TACAGCTGTC AGCTGCTAGT GTGGGTAATT GTCCCACAAA AACACATATG 
CCAGCTGTTA CTTCATCCCC AACCGTTAAT GAAAATGAAG CCAGTACAAA CAAATCTGGT 
TTTGAGCCTC ATGATTCTTC AGTGGTAGAT AATGAGGTAT GGTCAGATAT GCGAAGACAT 
GAAATGTTAA GGGAGGAGCT ACGACAGAGA AGAAAGCAGC TGGAAGCTCT GATGGCTGAA 
CATCAGAGGA GGCAAGGTCT GGCTGAAACT GCATCTCCAG TGGCTGTTTC ACTGAGAAGT 
GATGGATCTG AGAACCTGTG TACACCTCAG CAAAGCAGAA CAGAAAAAAC AATGGCAACT 
TGGGGAGGTT CTACCCAGTG TGCCCTAGAT GAAGAAGGAG ATGAAGATGG TTACCTTTCT 
GAAGGAATTA TTCGGACAGA CGAAGAGGAG GAAGAGGAGG AGCAAGATGC CAGTTCCAAT 
GATAACTTTC ATATGTATCC TCCAAACAGT GCAAATCATA ATTCTTACAA TGTAAAGGAA 
ACTAAAAATA GGTGGAAGAA CAATCGCCCT TTTTCAGCAG ATGGAAATTA TCGTCCTTTA 
GCCAAGACAA GACAGCAGAA TATCAGCATG CAGCGTCAGG AAAACCTTCG CTGGGTGTCC 
GAACTCTCTT ATGTAGAAGA GAAAGAACAA TGGCAAGAAC AAATTAATCA GCTAAAGAAA 
CAACTTGATT TCAGTGTCAA TATTTGTCAG ACTTTAATGC AAGACCAGCA GACTCTGTCT 
TGTCTGCTAC AAACTCTTCT CACGAGTCCT TATAGTGTTA TGCCCAGCAG TGTTGCATCC 
CCGCAAGTGC ACCTTATAAT GCACCAGTTG AATCAGTGCT ATACCCAGCT AACGTGGCAG 
CAGAACAATG TCCAGAGGTT GAAACAAATG CTCAGTGAAC TCATGCGCCA ACAAAATCAG 
CATCCAGAAA AACCTGCAAG CAAGGAAGGA GGCAGTGGTA CATCACACCC TCCTTCTCCC 
AGTTTATTTT GTCCTTTCAA CTTTCCAACT CAGCCTGTAA ATCTGTTTAA CCTACCGGGG 
TTTACTAATT TTTCGTCATT TGCACCAAGT ATGAATTTCA GCCCTTTATT TCCTTCTAAT 
TTTGGAGATT TTTCTCAAAA TATTTCTACA CCCACTGAAC AGCAGCAACC GTTAGCCCAG 
AATCCTTCAG GGAAAACGGA ATACATGGCT TTTCCAAAAC CTTTTGAAAG TAGTTCTTCT 
ATTGGAGCGG AAAAACAAAG GAATCAAAAA CAGCCTGAAG AGGAGGTGGA GAACAGTAGG 
ACACCATGGT TTTATGACCA AGAAGGTGAA GTAGAAAAGC CATTTCTCAA GACTGGATTT 
GCAGTGTCTG TAGAGAAAAC AACAAATAGT AATCGCAAAA ATCAACTAGA TACCAGTAGG 
AGAAGACGCC AGTTTGATGA AGTGTCCTTA GAAAGCTTTA ACAGTATGCT TGACCCAGTA 
GATCCAACAA CAGTAACTAA AACATTCAAG TCAAGAAAAG CATCTGCACA GGCCAGCCTG 
GCATCTAAAG ATAAAACTCC CAAATCAAAG AGTAAGAAGA GGCATTCTAC TCAGCTGAAA 
AGCAGAGTGA AAAATATCGG GTATGAAAGT GCCAGTGTGT CTAGCACATG TGAACCTTGC 
AAAAGTAGAA ACAGACATTC AGCCCAAACT GAAGAGCCTG TTCAAGCAAA AGTACTCAGC 
AGAAAGAATC ATGAGCAGCT GGAAAAAGTC ATAAGATGTA GTAGGTCTAC AGAAATATCT 
TCAGCACATG CTAGGAGAAT ACTACAGCAG TCTAACAGAA ATGCATGCAA TGAAGCGCCA 
GAAACCGGGA GTGATTTTTC CATGTTCGAA GCTTTGCGGG ATACCATTTA TTCTGAAGTA 
GCTACGTTGA TTTCTCAAAA TGAATCTCGT CCACATTTTC TTATTGAACT CTTCCATGAG 
CTTCAGCTAC TTAATACAGA CTATTTGAGA CAGAGGGCCT TATATGCATT GCAGGACATA 
GTATCCAGGC ATATTTCTGA GAACCATGAA AAAGAAGGAG AAAATATAAA GTCCGTGAAT 
TCTGGTACTT GGATAGCATC CAACTCAGAA CTTACTCCCA GTGAAAGCCT TGCTACTACT 
GATGATGAAA CTTTTGAGAA GAACTTTGAG AGAGAAACAC ATAAAATAAG TGAGCAAAAT 
GATGCTGATA ATGCTAGTGT ATCTTCAACT TGTGAGCCTT TTGCAACAGA TGTTCTGGGC 
GACACTGTGA TTCACTTAGA TCAGGCATTA GCCAGAATGA GAGACTATAC GCATATGAAG 
ATGGAGGCTG AAAGTAGTCC AGACGTCAGG TGCACCTGCA GGATCATTGA GGATGACGAT 
GGTGCTGCTG CCACAACCAC GATTAGTAAT AATTTAGAAG AAACTCCTGT TATTGAAAAT 
CATAGTTCAC AGCAACCCGT GAGTGAAGTT GCTACCGTCC CGTGTCCTAG AATTGATACT 
CAGCAACTGG ACCGGCAAAT TAAAGCAATT ATGAAAGAAG TCATTCCTTT TTTGAAGGAG 
CACATGGATG AAGTATGTTC TTCTCAACTT CTAACCTCAG TAAGACGCAT GGTTTTGGTC 
CTTACTCAGC AAAACAATGA GAGCAAAGAG TTTGTAAAGT TCTTTCATAA GCAGCTTGGA 
AGTATATTAC AGGATTCACT CGCAAAATTT GCTGGCAGAA AACTGAAGGA TTGTGGAGAA 
GATCTTCTTG TAGAGATATC TGAAGTGTTA TTTAATGAAT TGGCTTTCTT TAAGCTCATG 
CAAGATTTGG ATAATAATAG TGTAACTGTT AAACAGAGAT GCAAAAGGAA AATAGAAGCT 
GCTGGAGTGA TACAGTCTTA TGCAAAAGAG GCCAAAAGGA TTCTTGAAGG AGATCACAGT 
TCACCTGCTG GAGAAATTGA TGATGAAGAC AAAGACAAGG ATGAGACGGA AACAGTTAAG 
CAGACTCAAA CGTCTGAGGT TTATGATGGT GACGGCCCCA AAAATGTGAG ATCTGATGTT 
TCTGATCAAG AGGATGATGA AGAAAGTGAA GAATGTCCAG TGTCTATTAA TTTGTCCAAA 
GCTGAAACTC AGGCTTTGAC TAATTACGGA AGTGGAGAAG ATGAGAATGA AGATGAAGAA 
ATAGAAGAAT TTGAAGAGGG ACCTGTAGAT GTCCAGACGT CACTTCAGGC TAACACTGAA 
ACTACAGAAG AAAATGAACA CGATGATCAG GTTCCACAGC ATGATTTTGA AAAATCATCA 
GAAAGCAAAA ATGTCCCATC AGAACAAGAA CCCACTACTA GTAAAGACTT TCTTTTGATG 
ACAGGTGACC AGAATAGCAC TCCTGTGAAG CCCTGTTACC TCAGTATCGT GGAAAATGAG 
CAACCTTTAA ATAGCCCTGT CCAGAAGGAC ACTCTCACTA CCATCGACTC ATCCAGTCAG 
CCTAACCCTT TGCCTTTACC TTTAACTGAA ATTGAAACCT TGGTGCCTAG AGTCAAAGAA 
GTGAAATCTG CTCAGGAGAC TCCTGAAAGT TCTTTGGCTG GAAGTCCTGA TACTGAATCT 
CCAGTGTTAG TGAATGACTA TGAAGCAGAA TCTGGTAACA TAAGTCAAAA ATCTGATGAA 
GAAGACTTTG TGAAAGTTGA AGATTTACCA CTTAAACTGA CAATATATTC AGAGGCAGAT 
CTAAGGAAGA AAATGGCCGA AGAAGAACAG AAAAACCACT TATCTGGTGA AATATGTGCA 
ATGCAGACTG AAGAATTAGC TGGGAATTCT CAGACACTAA AAGAACCTGA GCAAGTATGT 
GCTCAGTCTG CCATCTTAAG ATGGAAACCC TTACCTGTGC CTTTTAAACT GTTGGTGGTG 
TCATCAGGGA ATTATTGTAG CAAAAAACAG TGGGAGCCCA AAGTACATGA GATGTCTCCA 
GAGGCTCAGC TAACTCTTTC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( XM_025279423.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025135208.1 Bubalus bubalis pericentriolar material 1 (PCM1), transcript variant X4, mRNA.

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CloneID	OBu42292
Clone ID Related Accession (Same CDS sequence)	XM_025279423.1
Accession Version	XM_025279423.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6669bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	pericentriolar material 1 protein isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037545.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG GAGGAGGTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
AGCAATGAGA GTGTTGATGA CCGACTCAAC AATATGGATT GGGGCAGCCA GCAGAAGAAG 
GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA 
ACTAACGATA TTTCTCCGGA GTCATCACCG GGAGTTGGAA GACGAAGAAC AAAGACTTCA 
CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TCCCCGAGCA GGCAGAACTA 
GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCTCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCCAC TGTAAGTCCT 
CAAAAGAGAG AAATGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAGATTCGTG AGTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGACCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTCACT 
CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT CCAGAAGATT 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCGGT CTGAGGCTTC AGATGCCACG 
GAGGCATCTT TTAGTCTGAG AATTCGGCCG TGCGTTGAGG ACAAACTAGG GAATTCAGCT 
TCACAAGCAC AGGTTTCAGA CACTGACGTT ACTACAAGTC CAAAAGGGAA AGGTGACAGA 
CCTCCTCAGC ATGACAGGGA GCTGAGGCCT AATAGGAAAT GTAGCCGAAA ACGTGGATTT 
CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATGGAAG AGATAGAAAA TTTGAAGAAA 
CAACATGATT TATTAAAAAG GATGTTACAG CAGCAGGAGC AGCTGAGAGC TCTACAAGGA 
CGACAAGCTG CTCTGCTTGC CCTGCAGCAT AAAGCTGAGC AGGCTATAGC TGTGATGGAT 
GATTCTGAAA AGGTTGCGGA GACAACTCCT GGCCATCATA CTGTACCAGG CAGACAGCCG 
GCTCATTCAC CTTTGCATCA GAGAGTACCC ATAAGAGAAA CTACAGGTAG CCTATCTGGA 
GTCAGTATCA CCTCTGAACT AAACGAAGAA TTGAATGATT TAATTCAGCA TTTTCATAAT 
CAGCTTCGTG ATTCTCAGCC TCCAGCTGTT CCAGACAATA GAAGACAAGC AGAAAGTCTT 
TCATTAACTA GGGAGATTTC TCAGAGCAGA AATCCTTCAG TTTCAGAGCA TTTACCTGAT 
GAGAAAGTCC AACTTTTTAG CAAGATGAGA GTGTTACAAG AAAAGAAACA AAAAATGGAC 
AAACTTCTTG GAGAACTTCA TACACTTCGA GATCAGCATT TGAACAATTC ATCATTTGTG 
CCTTCTTCAG CCTCTCCACA AAGGAGTGTG GATCAGAGAA GTACAACCAC AGCTCCTTCT 
GCTCCTGTAG TCTTAACACC AGTGGTCAAT GGAGAATCCA ATAACCTCAC GCCATCTGTT 
CCTTATCCTG CTGCTTCTCT AGTTTCTCAG AATCAGAATG AAAATGAAGG CCACCTAAAT 
CCAACTGAAA AACTCCAGAA GTTAAATGAA GTTCGAAAGA GGTTGAATGA ACTAAGAGAA 
TTAGTTCATT ATTATGAGCA AACATCAGAT ATGATGACAG ATGCTGTGAA TGAAAACACA 
AAAGAAGAAG AAACTGAAGA ATCAGAATAT GATTCGGAGC ATGAAAACTC GGAACCTGTT 
ACTAACATTC GAAATCCACA AGTGGCTGCC ACCTGGAACG AAGTAAATAG TAATTCTAAT 
GCACAGTGTG TTTCCAACAA TAGAGATGGG CGAGCAGTTA ATTCTAACTG TGAAATTAAC 
AACAGGTCTG CTGCCAACAT AAGGGCTCTA AACATGCCTC CTCTTTCAGC AGATTGTCGA 
TACAATAGAG AAGGAGAACA GGAGATGCCT GTTGCACAAG GTCATGAGGA GGAGGAGGAG 
GAGGAGGAGG AAGCAGAAGA TGAGGCCCTC AGTGGAGCTT CACTGTCTAG TCACAGCAGC 
AGCCTGATCA TTGAGACTCC TGAAGATGCT GAATTTGAAC ATAAGATCCA GAAGCTTATG 
GCTGCAAAAC TGAAACTTAG ACACTTACAG AACCTTGTAG CCATGGTGCA GGACGATGAT 
GCAGCCAATC AAGGAGTTAC TTCTGTGGAG GATTTCTTCC CAGCAGAAGA CACCAAACAA 
AATGCAAATA ACACTAGAGG AAATGCCAAT AAAACACAGA AAGATGCTGG AGTAAATGAA 
AAGGCAAGGG AGAAATTTTA TGAGGCTAAA CTACAGCAGC AACAGAGAGA ACTCAAACAA 
TTGCAGGAAG AAAGAAAGAA ACTGATTGAG ATTCAAGAGA AAATTCAAGA ATTGCAAAAG 
GCATGTCCTA ACCTACAGCT GTCAGCTGCT AGTGTGGGTA ATTGTCCCAC AAAAACACAT 
ATGCCAGCTG TTACTTCATC CCCAACCGTT AATGAAAATG AAGCCAGTAC AAACAAATCT 
GGTTTTGAGC CTCATGATTC TTCAGTGGTA GATAATGAGG TATGGTCAGA TATGCGAAGA 
CATGAAATGT TAAGGGAGGA GCTACGACAG AGAAGAAAGC AGCTGGAAGC TCTGATGGCT 
GAACATCAGA GGAGGCAAGG TCTGGCTGAA ACTGCATCTC CAGTGGCTGT TTCACTGAGA 
AGTGATGGAT CTGAGAACCT GTGTACACCT CAGCAAAGCA GAACAGAAAA AACAATGGCA 
ACTTGGGGAG GTTCTACCCA GTGTGCCCTA GATGAAGAAG GAGATGAAGA TGGTTACCTT 
TCTGAAGGAA TTATTCGGAC AGACGAAGAG GAGGAAGAGG AGGAGCAAGA TGCCAGTTCC 
AATGATAACT TTCATATGTA TCCTCCAAAC AGTGCAAATC ATAATTCTTA CAATGTAAAG 
GAAACTAAAA ATAGGTGGAA GAACAATCGC CCTTTTTCAG CAGATGGAAA TTATCGTCCT 
TTAGCCAAGA CAAGACAGCA GAATATCAGC ATGCAGCGTC AGGAAAACCT TCGCTGGGTG 
TCCGAACTCT CTTATGTAGA AGAGAAAGAA CAATGGCAAG AACAAATTAA TCAGCTAAAG 
AAACAACTTG ATTTCAGTGT CAATATTTGT CAGACTTTAA TGCAAGACCA GCAGACTCTG 
TCTTGTCTGC TACAAACTCT TCTCACGAGT CCTTATAGTG TTATGCCCAG CAGTGTTGCA 
TCCCCGCAAG TGCACCTTAT AATGCACCAG TTGAATCAGT GCTATACCCA GCTAACGTGG 
CAGCAGAACA ATGTCCAGAG GTTGAAACAA ATGCTCAGTG AACTCATGCG CCAACAAAAT 
CAGCATCCAG AAAAACCTGC AAGCAAGGAA GGAGGCAGTG GTACATCACA CCCTCCTTCT 
CCCAGTTTAT TTTGTCCTTT CAACTTTCCA ACTCAGCCTG TAAATCTGTT TAACCTACCG 
GGGTTTACTA ATTTTTCGTC ATTTGCACCA AGTATGAATT TCAGCCCTTT ATTTCCTTCT 
AATTTTGGAG ATTTTTCTCA AAATATTTCT ACACCCACTG AACAGCAGCA ACCGTTAGCC 
CAGAATCCTT CAGGGAAAAC GGAATACATG GCTTTTCCAA AACCTTTTGA AAGTAGTTCT 
TCTATTGGAG CGGAAAAACA AAGGAATCAA AAACAGCCTG AAGAGGAGGT GGAGAACAGT 
AGGACACCAT GGTTTTATGA CCAAGAAGGT GAAGTAGAAA AGCCATTTCT CAAGACTGGA 
TTTGCAGTGT CTGTAGAGAA AACAACAAAT AGTAATCGCA AAAATCAACT AGATACCAGT 
AGGAGAAGAC GCCAGTTTGA TGAAGTGTCC TTAGAAAGCT TTAACAGTAT GCTTGACCCA 
GTAGATCCAA CAACAGTAAC TAAAACATTC AAGTCAAGAA AAGCATCTGC ACAGGCCAGC 
CTGGCATCTA AAGATAAAAC TCCCAAATCA AAGAGTAAGA AGAGGCATTC TACTCAGCTG 
AAAAGCAGAG TGAAAAATAT CGGGTATGAA AGTGCCAGTG TGTCTAGCAC ATGTGAACCT 
TGCAAAAGTA GAAACAGACA TTCAGCCCAA ACTGAAGAGC CTGTTCAAGC AAAAGTACTC 
AGCAGAAAGA ATCATGAGCA GCTGGAAAAA GTCATAAGAT GTAGTAGGTC TACAGAAATA 
TCTTCAGCAC ATGCTAGGAG AATACTACAG CAGTCTAACA GAAATGCATG CAATGAAGCG 
CCAGAAACCG GGAGTGATTT TTCCATGTTC GAAGCTTTGC GGGATACCAT TTATTCTGAA 
GTAGCTACGT TGATTTCTCA AAATGAATCT CGTCCACATT TTCTTATTGA ACTCTTCCAT 
GAGCTTCAGC TACTTAATAC AGACTATTTG AGACAGAGGG CCTTATATGC ATTGCAGGAC 
ATAGTATCCA GGCATATTTC TGAGAACCAT GAAAAAGAAG GAGAAAATAT AAAGTCCGTG 
AATTCTGGTA CTTGGATAGC ATCCAACTCA GAACTTACTC CCAGTGAAAG CCTTGCTACT 
ACTGATGATG AAACTTTTGA GAAGAACTTT GAGAGAGAAA CACATAAAAT AAGTGAGCAA 
AATGATGCTG ATAATGCTAG TGTATCTTCA ACTTGTGAGC CTTTTGCAAC AGATGTTCTG 
GGCGACACTG TGATTCACTT AGATCAGGCA TTAGCCAGAA TGAGAGACTA TACGCATATG 
AAGATGGAGG CTGAAAGTAG TCCAGACGTC AGGTGCACCT GCAGGATCAT TGAGGATGAC 
GATGGTGCTG CTGCCACAAC CACGATTAGT AATAATTTAG AAGAAACTCC TGTTATTGAA 
AATCATAGTT CACAGCAACC CGTGAGTGAA GTTGCTACCG TCCCGTGTCC TAGAATTGAT 
ACTCAGCAAC TGGACCGGCA AATTAAAGCA ATTATGAAAG AAGTCATTCC TTTTTTGAAG 
GAGCACATGG ATGAAGTATG TTCTTCTCAA CTTCTAACCT CAGTAAGACG CATGGTTTTG 
GTCCTTACTC AGCAAAACAA TGAGAGCAAA GAGTTTGTAA AGTTCTTTCA TAAGCAGCTT 
GGAAGTATAT TACAGGATTC ACTCGCAAAA TTTGCTGGCA GAAAACTGAA GGATTGTGGA 
GAAGATCTTC TTGTAGAGAT ATCTGAAGTG TTATTTAATG AATTGGCTTT CTTTAAGCTC 
ATGCAAGATT TGGATAATAA TAGTGTAACT GTTAAACAGA GATGCAAAAG GAAAATAGAA 
GCTGCTGGAG TGATACAGTC TTATGCAAAA GAGGCCAAAA GGATTCTTGA AGGAGATCAC 
AGTTCACCTG CTGGAGAAAT TGATGATGAA GACAAAGACA AGGATGAGAC GGAAACAGTT 
AAGCAGACTC AAACGTCTGA GGTTTATGAT GGTGACGGCC CCAAAAATGT GAGATCTGAT 
GTTTCTGATC AAGAGGATGA TGAAGAAAGT GAAGAATGTC CAGTGTCTAT TAATTTGTCC 
AAAGCTGAAA CTCAGGCTTT GACTAATTAC GGAAGTGGAG AAGATGAGAA TGAAGATGAA 
GAAATAGAAG AATTTGAAGA GGGACCTGTA GATGTCCAGA CGTCACTTCA GGCTAACACT 
GAAACTACAG AAGAAAATGA ACACGATGAT CAGGTTCCAC AGCATGATTT TGAAAAATCA 
TCAGAAAGCA AAAATGTCCC ATCAGAACAA GAACCCACTA CTAGTAAAGG TGACCAGAAT 
AGCACTCCTG TGAAGCCCTG TTACCTCAGT ATCGTGGAAA ATGAGCAACC TTTAAATAGC 
CCTGTCCAGA AGGACACTCT CACTACCATC GACTCATCCA GTCAGCCTAA CCCTTTGCCT 
TTACCTTTAA CTGAAATTGA AACCTTGGTG CCTAGAGTCA AAGAAGTGAA ATCTGCTCAG 
GAGACTCCTG AAAGTTCTTT GGCTGGAAGT CCTGATACTG AATCTCCAGT GTTAGTGAAT 
GACTATGAAG CAGAATCTGG TAACATAAGT CAAAAATCTG ATGAAGAAGA CTTTGTGAAA 
GTTGAAGATT TACCACTTAA ACTGACAATA TATTCAGAGG CAGATCTAAG GAAGAAAATG 
GCCGAAGAAG AACAGAAAAA CCACTTATCT GGTGAAATAT GTGCAATGCA GACTGAAGAA 
TTAGCTGGGA ATTCTCAGAC ACTAAAAGAA CCTGAGCAAG TATGTGCTCA GTCTGCCATC 
TTAAGATGGA AACCCTTACC TGTGCCTTTT AAACTGTTGG TGGTGTCATC AGGGAATTAT 
TGTAGCAAAA AACAGTGGGA GCCCAAAGTA CATGAGATGT CTCCAGAGGC TCAGCTAACT 
CTTTCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025135208.1
CDS	392..7060
Translation	MATGGGPFEEGVNDQDLPNWSNESVDDRLNNMDWGSQQKKANRSSEKNKRKFGVESDKRVTNDISPESSPGVGRRRTKTSLTFPHSRYMTQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDATVSPQKREMIGSAQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIREYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLQKILARENEEEDVRTIDSAVGSGSVAESTSLNIDVRSEASDATEASFSLRIRPCVEDKLGNSASQAQVSDTDVTTSPKGKGDRPPQHDRELRPNRKCSRKRGFPSKARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSEKVAETTPGHHTVPGRQPAHSPLHQRVPIRETTGSLSGVSITSELNEELNDLIQHFHNQLRDSQPPAVPDNRRQAESLSLTREISQSRNPSVSEHLPDEKVQLFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSFVPSSASPQRSVDQRSTTTAPSAPVVLTPVVNGESNNLTPSVPYPAASLVSQNQNENEGHLNPTEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENTKEEETEESEYDSEHENSEPVTNIRNPQVAATWNEVNSNSNAQCVSNNRDGRAVNSNCEINNRSAANIRALNMPPLSADCRYNREGEQEMPVAQGHEEEEEEEEEAEDEALSGASLSSHSSSLIIETPEDAEFEHKIQKLMAAKLKLRHLQNLVAMVQDDDAANQGVTSVEDFFPAEDTKQNANNTRGNANKTQKDAGVNEKAREKFYEAKLQQQQRELKQLQEERKKLIEIQEKIQELQKACPNLQLSAASVGNCPTKTHMPAVTSSPTVNENEASTNKSGFEPHDSSVVDNEVWSDMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETASPVAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGIIRTDEEEEEEEQDASSNDNFHMYPPNSANHNSYNVKETKNRWKNNRPFSADGNYRPLAKTRQQNISMQRQENLRWVSELSYVEEKEQWQEQINQLKKQLDFSVNICQTLMQDQQTLSCLLQTLLTSPYSVMPSSVASPQVHLIMHQLNQCYTQLTWQQNNVQRLKQMLSELMRQQNQHPEKPASKEGGSGTSHPPSPSLFCPFNFPTQPVNLFNLPGFTNFSSFAPSMNFSPLFPSNFGDFSQNISTPTEQQQPLAQNPSGKTEYMAFPKPFESSSSIGAEKQRNQKQPEEEVENSRTPWFYDQEGEVEKPFLKTGFAVSVEKTTNSNRKNQLDTSRRRRQFDEVSLESFNSMLDPVDPTTVTKTFKSRKASAQASLASKDKTPKSKSKKRHSTQLKSRVKNIGYESASVSSTCEPCKSRNRHSAQTEEPVQAKVLSRKNHEQLEKVIRCSRSTEISSAHARRILQQSNRNACNEAPETGSDFSMFEALRDTIYSEVATLISQNESRPHFLIELFHELQLLNTDYLRQRALYALQDIVSRHISENHEKEGENIKSVNSGTWIASNSELTPSESLATTDDETFEKNFERETHKISEQNDADNASVSSTCEPFATDVLGDTVIHLDQALARMRDYTHMKMEAESSPDVRCTCRIIEDDDGAAATTTISNNLEETPVIENHSSQQPVSEVATVPCPRIDTQQLDRQIKAIMKEVIPFLKEHMDEVCSSQLLTSVRRMVLVLTQQNNESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSVTVKQRCKRKIEAAGVIQSYAKEAKRILEGDHSSPAGEIDDEDKDKDETETVKQTQTSEVYDGDGPKNVRSDVSDQEDDEESEECPVSINLSKAETQALTNYGSGEDENEDEEIEEFEEGPVDVQTSLQANTETTEENEHDDQVPQHDFEKSSESKNVPSEQEPTTSKGDQNSTPVKPCYLSIVENEQPLNSPVQKDTLTTIDSSSQPNPLPLPLTEIETLVPRVKEVKSAQETPESSLAGSPDTESPVLVNDYEAESGNISQKSDEEDFVKVEDLPLKLTIYSEADLRKKMAEEEQKNHLSGEICAMQTEELAGNSQTLKEPEQVCAQSAILRWKPLPVPFKLLVVSSGNYCSKKQWEPKVHEMSPEAQLTLS

Target ORF information:

RefSeq Version	XM_025279423.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis pericentriolar material 1 (PCM1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025279423.1

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
AGCAATGAGA GTGTTGATGA CCGACTCAAC AATATGGATT GGGGCAGCCA GCAGAAGAAG 
GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA 
ACTAACGATA TTTCTCCGGA GTCATCACCG GGAGTTGGAA GACGAAGAAC AAAGACTTCA 
CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TCCCCGAGCA GGCAGAACTA 
GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCTCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCCAC TGTAAGTCCT 
CAAAAGAGAG AAATGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAGATTCGTG AGTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGACCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTCACT 
CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT CCAGAAGATT 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCGGT CTGAGGCTTC AGATGCCACG 
GAGGCATCTT TTAGTCTGAG AATTCGGCCG TGCGTTGAGG ACAAACTAGG GAATTCAGCT 
TCACAAGCAC AGGTTTCAGA CACTGACGTT ACTACAAGTC CAAAAGGGAA AGGTGACAGA 
CCTCCTCAGC ATGACAGGGA GCTGAGGCCT AATAGGAAAT GTAGCCGAAA ACGTGGATTT 
CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATGGAAG AGATAGAAAA TTTGAAGAAA 
CAACATGATT TATTAAAAAG GATGTTACAG CAGCAGGAGC AGCTGAGAGC TCTACAAGGA 
CGACAAGCTG CTCTGCTTGC CCTGCAGCAT AAAGCTGAGC AGGCTATAGC TGTGATGGAT 
GATTCTGAAA AGGTTGCGGA GACAACTCCT GGCCATCATA CTGTACCAGG CAGACAGCCG 
GCTCATTCAC CTTTGCATCA GAGAGTACCC ATAAGAGAAA CTACAGGTAG CCTATCTGGA 
GTCAGTATCA CCTCTGAACT AAACGAAGAA TTGAATGATT TAATTCAGCA TTTTCATAAT 
CAGCTTCGTG ATTCTCAGCC TCCAGCTGTT CCAGACAATA GAAGACAAGC AGAAAGTCTT 
TCATTAACTA GGGAGATTTC TCAGAGCAGA AATCCTTCAG TTTCAGAGCA TTTACCTGAT 
GAGAAAGTCC AACTTTTTAG CAAGATGAGA GTGTTACAAG AAAAGAAACA AAAAATGGAC 
AAACTTCTTG GAGAACTTCA TACACTTCGA GATCAGCATT TGAACAATTC ATCATTTGTG 
CCTTCTTCAG CCTCTCCACA AAGGAGTGTG GATCAGAGAA GTACAACCAC AGCTCCTTCT 
GCTCCTGTAG TCTTAACACC AGTGGTCAAT GGAGAATCCA ATAACCTCAC GCCATCTGTT 
CCTTATCCTG CTGCTTCTCT AGTTTCTCAG AATCAGAATG AAAATGAAGG CCACCTAAAT 
CCAACTGAAA AACTCCAGAA GTTAAATGAA GTTCGAAAGA GGTTGAATGA ACTAAGAGAA 
TTAGTTCATT ATTATGAGCA AACATCAGAT ATGATGACAG ATGCTGTGAA TGAAAACACA 
AAAGAAGAAG AAACTGAAGA ATCAGAATAT GATTCGGAGC ATGAAAACTC GGAACCTGTT 
ACTAACATTC GAAATCCACA AGTGGCTGCC ACCTGGAACG AAGTAAATAG TAATTCTAAT 
GCACAGTGTG TTTCCAACAA TAGAGATGGG CGAGCAGTTA ATTCTAACTG TGAAATTAAC 
AACAGGTCTG CTGCCAACAT AAGGGCTCTA AACATGCCTC CTCTTTCAGC AGATTGTCGA 
TACAATAGAG AAGGAGAACA GGAGATGCCT GTTGCACAAG GTCATGAGGA GGAGGAGGAG 
GAGGAGGAGG AAGCAGAAGA TGAGGCCCTC AGTGGAGCTT CACTGTCTAG TCACAGCAGC 
AGCCTGATCA TTGAGACTCC TGAAGATGCT GAATTTGAAC ATAAGATCCA GAAGCTTATG 
GCTGCAAAAC TGAAACTTAG ACACTTACAG AACCTTGTAG CCATGGTGCA GGACGATGAT 
GCAGCCAATC AAGGAGTTAC TTCTGTGGAG GATTTCTTCC CAGCAGAAGA CACCAAACAA 
AATGCAAATA ACACTAGAGG AAATGCCAAT AAAACACAGA AAGATGCTGG AGTAAATGAA 
AAGGCAAGGG AGAAATTTTA TGAGGCTAAA CTACAGCAGC AACAGAGAGA ACTCAAACAA 
TTGCAGGAAG AAAGAAAGAA ACTGATTGAG ATTCAAGAGA AAATTCAAGA ATTGCAAAAG 
GCATGTCCTA ACCTACAGCT GTCAGCTGCT AGTGTGGGTA ATTGTCCCAC AAAAACACAT 
ATGCCAGCTG TTACTTCATC CCCAACCGTT AATGAAAATG AAGCCAGTAC AAACAAATCT 
GGTTTTGAGC CTCATGATTC TTCAGTGGTA GATAATGAGG TATGGTCAGA TATGCGAAGA 
CATGAAATGT TAAGGGAGGA GCTACGACAG AGAAGAAAGC AGCTGGAAGC TCTGATGGCT 
GAACATCAGA GGAGGCAAGG TCTGGCTGAA ACTGCATCTC CAGTGGCTGT TTCACTGAGA 
AGTGATGGAT CTGAGAACCT GTGTACACCT CAGCAAAGCA GAACAGAAAA AACAATGGCA 
ACTTGGGGAG GTTCTACCCA GTGTGCCCTA GATGAAGAAG GAGATGAAGA TGGTTACCTT 
TCTGAAGGAA TTATTCGGAC AGACGAAGAG GAGGAAGAGG AGGAGCAAGA TGCCAGTTCC 
AATGATAACT TTCATATGTA TCCTCCAAAC AGTGCAAATC ATAATTCTTA CAATGTAAAG 
GAAACTAAAA ATAGGTGGAA GAACAATCGC CCTTTTTCAG CAGATGGAAA TTATCGTCCT 
TTAGCCAAGA CAAGACAGCA GAATATCAGC ATGCAGCGTC AGGAAAACCT TCGCTGGGTG 
TCCGAACTCT CTTATGTAGA AGAGAAAGAA CAATGGCAAG AACAAATTAA TCAGCTAAAG 
AAACAACTTG ATTTCAGTGT CAATATTTGT CAGACTTTAA TGCAAGACCA GCAGACTCTG 
TCTTGTCTGC TACAAACTCT TCTCACGAGT CCTTATAGTG TTATGCCCAG CAGTGTTGCA 
TCCCCGCAAG TGCACCTTAT AATGCACCAG TTGAATCAGT GCTATACCCA GCTAACGTGG 
CAGCAGAACA ATGTCCAGAG GTTGAAACAA ATGCTCAGTG AACTCATGCG CCAACAAAAT 
CAGCATCCAG AAAAACCTGC AAGCAAGGAA GGAGGCAGTG GTACATCACA CCCTCCTTCT 
CCCAGTTTAT TTTGTCCTTT CAACTTTCCA ACTCAGCCTG TAAATCTGTT TAACCTACCG 
GGGTTTACTA ATTTTTCGTC ATTTGCACCA AGTATGAATT TCAGCCCTTT ATTTCCTTCT 
AATTTTGGAG ATTTTTCTCA AAATATTTCT ACACCCACTG AACAGCAGCA ACCGTTAGCC 
CAGAATCCTT CAGGGAAAAC GGAATACATG GCTTTTCCAA AACCTTTTGA AAGTAGTTCT 
TCTATTGGAG CGGAAAAACA AAGGAATCAA AAACAGCCTG AAGAGGAGGT GGAGAACAGT 
AGGACACCAT GGTTTTATGA CCAAGAAGGT GAAGTAGAAA AGCCATTTCT CAAGACTGGA 
TTTGCAGTGT CTGTAGAGAA AACAACAAAT AGTAATCGCA AAAATCAACT AGATACCAGT 
AGGAGAAGAC GCCAGTTTGA TGAAGTGTCC TTAGAAAGCT TTAACAGTAT GCTTGACCCA 
GTAGATCCAA CAACAGTAAC TAAAACATTC AAGTCAAGAA AAGCATCTGC ACAGGCCAGC 
CTGGCATCTA AAGATAAAAC TCCCAAATCA AAGAGTAAGA AGAGGCATTC TACTCAGCTG 
AAAAGCAGAG TGAAAAATAT CGGGTATGAA AGTGCCAGTG TGTCTAGCAC ATGTGAACCT 
TGCAAAAGTA GAAACAGACA TTCAGCCCAA ACTGAAGAGC CTGTTCAAGC AAAAGTACTC 
AGCAGAAAGA ATCATGAGCA GCTGGAAAAA GTCATAAGAT GTAGTAGGTC TACAGAAATA 
TCTTCAGCAC ATGCTAGGAG AATACTACAG CAGTCTAACA GAAATGCATG CAATGAAGCG 
CCAGAAACCG GGAGTGATTT TTCCATGTTC GAAGCTTTGC GGGATACCAT TTATTCTGAA 
GTAGCTACGT TGATTTCTCA AAATGAATCT CGTCCACATT TTCTTATTGA ACTCTTCCAT 
GAGCTTCAGC TACTTAATAC AGACTATTTG AGACAGAGGG CCTTATATGC ATTGCAGGAC 
ATAGTATCCA GGCATATTTC TGAGAACCAT GAAAAAGAAG GAGAAAATAT AAAGTCCGTG 
AATTCTGGTA CTTGGATAGC ATCCAACTCA GAACTTACTC CCAGTGAAAG CCTTGCTACT 
ACTGATGATG AAACTTTTGA GAAGAACTTT GAGAGAGAAA CACATAAAAT AAGTGAGCAA 
AATGATGCTG ATAATGCTAG TGTATCTTCA ACTTGTGAGC CTTTTGCAAC AGATGTTCTG 
GGCGACACTG TGATTCACTT AGATCAGGCA TTAGCCAGAA TGAGAGACTA TACGCATATG 
AAGATGGAGG CTGAAAGTAG TCCAGACGTC AGGTGCACCT GCAGGATCAT TGAGGATGAC 
GATGGTGCTG CTGCCACAAC CACGATTAGT AATAATTTAG AAGAAACTCC TGTTATTGAA 
AATCATAGTT CACAGCAACC CGTGAGTGAA GTTGCTACCG TCCCGTGTCC TAGAATTGAT 
ACTCAGCAAC TGGACCGGCA AATTAAAGCA ATTATGAAAG AAGTCATTCC TTTTTTGAAG 
GAGCACATGG ATGAAGTATG TTCTTCTCAA CTTCTAACCT CAGTAAGACG CATGGTTTTG 
GTCCTTACTC AGCAAAACAA TGAGAGCAAA GAGTTTGTAA AGTTCTTTCA TAAGCAGCTT 
GGAAGTATAT TACAGGATTC ACTCGCAAAA TTTGCTGGCA GAAAACTGAA GGATTGTGGA 
GAAGATCTTC TTGTAGAGAT ATCTGAAGTG TTATTTAATG AATTGGCTTT CTTTAAGCTC 
ATGCAAGATT TGGATAATAA TAGTGTAACT GTTAAACAGA GATGCAAAAG GAAAATAGAA 
GCTGCTGGAG TGATACAGTC TTATGCAAAA GAGGCCAAAA GGATTCTTGA AGGAGATCAC 
AGTTCACCTG CTGGAGAAAT TGATGATGAA GACAAAGACA AGGATGAGAC GGAAACAGTT 
AAGCAGACTC AAACGTCTGA GGTTTATGAT GGTGACGGCC CCAAAAATGT GAGATCTGAT 
GTTTCTGATC AAGAGGATGA TGAAGAAAGT GAAGAATGTC CAGTGTCTAT TAATTTGTCC 
AAAGCTGAAA CTCAGGCTTT GACTAATTAC GGAAGTGGAG AAGATGAGAA TGAAGATGAA 
GAAATAGAAG AATTTGAAGA GGGACCTGTA GATGTCCAGA CGTCACTTCA GGCTAACACT 
GAAACTACAG AAGAAAATGA ACACGATGAT CAGGTTCCAC AGCATGATTT TGAAAAATCA 
TCAGAAAGCA AAAATGTCCC ATCAGAACAA GAACCCACTA CTAGTAAAGG TGACCAGAAT 
AGCACTCCTG TGAAGCCCTG TTACCTCAGT ATCGTGGAAA ATGAGCAACC TTTAAATAGC 
CCTGTCCAGA AGGACACTCT CACTACCATC GACTCATCCA GTCAGCCTAA CCCTTTGCCT 
TTACCTTTAA CTGAAATTGA AACCTTGGTG CCTAGAGTCA AAGAAGTGAA ATCTGCTCAG 
GAGACTCCTG AAAGTTCTTT GGCTGGAAGT CCTGATACTG AATCTCCAGT GTTAGTGAAT 
GACTATGAAG CAGAATCTGG TAACATAAGT CAAAAATCTG ATGAAGAAGA CTTTGTGAAA 
GTTGAAGATT TACCACTTAA ACTGACAATA TATTCAGAGG CAGATCTAAG GAAGAAAATG 
GCCGAAGAAG AACAGAAAAA CCACTTATCT GGTGAAATAT GTGCAATGCA GACTGAAGAA 
TTAGCTGGGA ATTCTCAGAC ACTAAAAGAA CCTGAGCAAG TATGTGCTCA GTCTGCCATC 
TTAAGATGGA AACCCTTACC TGTGCCTTTT AAACTGTTGG TGGTGTCATC AGGGAATTAT 
TGTAGCAAAA AACAGTGGGA GCCCAAAGTA CATGAGATGT CTCCAGAGGC TCAGCTAACT 
CTTTCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( XM_025279429.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025135214.1 Bubalus bubalis pericentriolar material 1 (PCM1), transcript variant X5, mRNA.

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CloneID	OBu42293
Clone ID Related Accession (Same CDS sequence)	XM_025279429.1
Accession Version	XM_025279429.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6630bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	pericentriolar material 1 protein isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037545.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG GAGGAGGTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
AGCAATGAGA GTGTTGATGA CCGACTCAAC AATATGGATT GGGGCAGCCA GCAGAAGAAG 
GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA 
ACTAACGATA TTTCTCCGGA GTCATCACCG GGAGTTGGAA GACGAAGAAC AAAGACTTCA 
CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TCCCCGAGCA GGCAGAACTA 
GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCTCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCCAC TGTAAGTCCT 
CAAAAGAGAG AAATGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAGATTCGTG AGTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGACCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTCACT 
CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT CCAGAAGATT 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCGGT CTGAGGCTTC AGATGCCACG 
GAGGCATCTT TTAGTCTGAG AATTCGGCCG TGCGTTGAGG ACAAACTAGG GAATTCAGCT 
TCACAAGCAC AGGTTTCAGA CACTGACGTT ACTACAAGTC CAAAAGGGAA AGGTGACAGA 
CCTCCTCAGC ATGACAGGGA GCTGAGGCCT AATAGGAAAT GTAGCCGAAA ACGTGGATTT 
CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATGGAAG AGATAGAAAA TTTGAAGAAA 
CAACATGATT TATTAAAAAG GATGTTACAG CAGCAGGAGC AGCTGAGAGC TCTACAAGGA 
CGACAAGCTG CTCTGCTTGC CCTGCAGCAT AAAGCTGAGC AGGCTATAGC TGTGATGGAT 
GATTCTGAAA AGGTTGCGGA GACAACTCCT GGCCATCATA CTGTACCAGG CAGACAGCCG 
GCTCATTCAC CTTTGCATCA GAGAGTACCC ATAAGAGAAA CTACAGGTAG CCTATCTGGA 
GTCAGTATCA CCTCTGAACT AAACGAAGAA TTGAATGATT TAATTCAGCA TTTTCATAAT 
CAGCTTCGTG ATTCTCAGCC TCCAGCTGTT CCAGACAATA GAAGACAAGC AGAAAGTCTT 
TCATTAACTA GGGAGATTTC TCAGAGCAGA AATCCTTCAG TTTCAGAGCA TTTACCTGAT 
GAGAAAGTCC AACTTTTTAG CAAGATGAGA GTGTTACAAG AAAAGAAACA AAAAATGGAC 
AAACTTCTTG GAGAACTTCA TACACTTCGA GATCAGCATT TGAACAATTC ATCATTTGTG 
CCTTCTTCAG CCTCTCCACA AAGGAGTGTG GATCAGAGAA GTACAACCAC AGCTCCTTCT 
GCTCCTGTAG TCTTAACACC AGTGGTCAAT GGAGAATCCA ATAACCTCAC GCCATCTGTT 
CCTTATCCTG CTGCTTCTCT AGTTTCTCAG AATCAGAATG AAAATGAAGG CCACCTAAAT 
CCAACTGAAA AACTCCAGAA GTTAAATGAA GTTCGAAAGA GGTTGAATGA ACTAAGAGAA 
TTAGTTCATT ATTATGAGCA AACATCAGAT ATGATGACAG ATGCTGTGAA TGAAAACACA 
AAAGAAGAAG AAACTGAAGA ATCAGAATAT GATTCGGAGC ATGAAAACTC GGAACCTGTT 
ACTAACATTC GAAATCCACA AGTGGCTGCC ACCTGGAACG AAGTAAATAG TAATTCTAAT 
GCACAGTGTG TTTCCAACAA TAGAGATGGG CGAGCAGTTA ATTCTAACTG TGAAATTAAC 
AACAGGTCTG CTGCCAACAT AAGGGCTCTA AACATGCCTC CTCTTTCAGC AGATTGTCGA 
TACAATAGAG AAGGAGAACA GGAGATGCCT GTTGCACAAG GTCATGAGGA GGAGGAGGAG 
GAGGAGGAGG AAGCAGAAGA TGAGGCCCTC AGTGGAGCTT CACTGTCTAG TCACAGCAGC 
AGCCTGATCA TTGAGACTCC TGAAGATGCT GAATTTGAAC ATAAGATCCA GAAGCTTATG 
GCTGCAAAAC TGAAACTTAG ACACTTACAG AACCTTGTAG CCATGGTGCA GGACGATGAT 
GCAGCCAATC AAGGAGTTAC TTCTGTGGAG GATTTCTTCC CAGCAGAAGA CACCAAACAA 
AATGCAAATA ACACTAGAGG AAATGCCAAT AAAACACAGA AAGATGCTGG AGTAAATGAA 
AAGGCAAGGG AGAAATTTTA TGAGGCTAAA CTACAGCAGC AACAGAGAGA ACTCAAACAA 
TTGCAGGAAG AAAGAAAGAA ACTGATTGAG ATTCAAGAGA AAATTCAAGA ATTGCAAAAG 
GCATGTCCTA ACCTACAGCT GTCAGCTGCT AGTGTGGGTA ATTGTCCCAC AAAAACACAT 
ATGCCAGCTG TTACTTCATC CCCAACCGTT AATGAAAATG AAGCCAGTAC AAACAAATCT 
GGTTTTGAGC CTCATGATTC TTCAGTGGTA GATAATGAGG TATGGTCAGA TATGCGAAGA 
CATGAAATGT TAAGGGAGGA GCTACGACAG AGAAGAAAGC AGCTGGAAGC TCTGATGGCT 
GAACATCAGA GGAGGCAAGG TCTGGCTGAA ACTGCATCTC CAGTGGCTGT TTCACTGAGA 
AGTGATGGAT CTGAGAACCT GTGTACACCT CAGCAAAGCA GAACAGAAAA AACAATGGCA 
ACTTGGGGAG GTTCTACCCA GTGTGCCCTA GATGAAGAAG GAGATGAAGA TGGTTACCTT 
TCTGAAGGAA TTATTCGGAC AGACGAAGAG GAGGAAGAGG AGGAGCAAGA TGCCAGTTCC 
AATGATAACT TTCATATGTA TCCTCCAAAC AGTGCAAATC ATAATTCTTA CAATGTAAAG 
GAAACTAAAA ATAGGTGGAA GAACAATCGC CCTTTTTCAG CAGATGGAAA TTATCGTCCT 
TTAGCCAAGA CAAGACAGCA GAATATCAGC ATGCAGCGTC AGGAAAACCT TCGCTGGGTG 
TCCGAACTCT CTTATGTAGA AGAGAAAGAA CAATGGCAAG AACAAATTAA TCAGCTAAAG 
AAACAACTTG ATTTCAGTGT CAATATTTGT CAGACTTTAA TGCAAGACCA GCAGACTCTG 
TCTTGTCTGC TACAAACTCT TCTCACGAGT CCTTATAGTG TTATGCCCAG CAGTGTTGCA 
TCCCCGCAAG TGCACCTTAT AATGCACCAG TTGAATCAGT GCTATACCCA GCTAACGTGG 
CAGCAGAACA ATGTCCAGAG GTTGAAACAA ATGCTCAGTG AACTCATGCG CCAACAAAAT 
CAGCATCCAG AAAAACCTGC AAGCAAGGAA GGAGGCAGTG GTACATCACA CCCTCCTTCT 
CCCAGTTTAT TTTGTCCTTT CAACTTTCCA ACTCAGCCTG TAAATCTGTT TAACCTACCG 
GGGTTTACTA ATTTTTCGTC ATTTGCACCA AGTATGAATT TCAGCCCTTT ATTTCCTTCT 
AATTTTGGAG ATTTTTCTCA AAATATTTCT ACACCCACTG AACAGCAGCA ACCGTTAGCC 
CAGAATCCTT CAGGGAAAAC GGAATACATG GCTTTTCCAA AACCTTTTGA AAGTAGTTCT 
TCTATTGGAG CGGAAAAACA AAGGAATCAA AAACAGCCTG AAGAGGAGGT GGAGAACAGT 
AGGACACCAT GGTTTTATGA CCAAGAAGGT GAAGTAGAAA AGCCATTTCT CAAGACTGGA 
TTTGCAGTGT CTGTAGAGAA AACAACAAAT AGTAATCGCA AAAATCAACT AGATACCAGT 
AGGAGAAGAC GCCAGTTTGA TGAAGTGTCC TTAGAAAGCT TTAACAGTAT GCTTGACCCA 
GTAGATCCAA CAACAGTAAC TAAAACATTC AAGTCAAGAA AAGCATCTGC ACAGGCCAGC 
CTGGCATCTA AAGATAAAAC TCCCAAATCA AAGAGTAAGA AGAGGCATTC TACTCAGCTG 
AAAAGCAGAG TGAAAAATAT CGGGTATGAA AGTGCCAGTG TGTCTAGCAC ATGTGAACCT 
TGCAAAAGTA GAAACAGACA TTCAGCCCAA ACTGAAGAGC CTGTTCAAGC AAAAGTACTC 
AGCAGAAAGA ATCATGAGCA GCTGGAAAAA GTCATAAGAT GTAGTAGGTC TACAGAAATA 
TCTTCAGAAA CCGGGAGTGA TTTTTCCATG TTCGAAGCTT TGCGGGATAC CATTTATTCT 
GAAGTAGCTA CGTTGATTTC TCAAAATGAA TCTCGTCCAC ATTTTCTTAT TGAACTCTTC 
CATGAGCTTC AGCTACTTAA TACAGACTAT TTGAGACAGA GGGCCTTATA TGCATTGCAG 
GACATAGTAT CCAGGCATAT TTCTGAGAAC CATGAAAAAG AAGGAGAAAA TATAAAGTCC 
GTGAATTCTG GTACTTGGAT AGCATCCAAC TCAGAACTTA CTCCCAGTGA AAGCCTTGCT 
ACTACTGATG ATGAAACTTT TGAGAAGAAC TTTGAGAGAG AAACACATAA AATAAGTGAG 
CAAAATGATG CTGATAATGC TAGTGTATCT TCAACTTGTG AGCCTTTTGC AACAGATGTT 
CTGGGCGACA CTGTGATTCA CTTAGATCAG GCATTAGCCA GAATGAGAGA CTATACGCAT 
ATGAAGATGG AGGCTGAAAG TAGTCCAGAC GTCAGGTGCA CCTGCAGGAT CATTGAGGAT 
GACGATGGTG CTGCTGCCAC AACCACGATT AGTAATAATT TAGAAGAAAC TCCTGTTATT 
GAAAATCATA GTTCACAGCA ACCCGTGAGT GAAGTTGCTA CCGTCCCGTG TCCTAGAATT 
GATACTCAGC AACTGGACCG GCAAATTAAA GCAATTATGA AAGAAGTCAT TCCTTTTTTG 
AAGGAGCACA TGGATGAAGT ATGTTCTTCT CAACTTCTAA CCTCAGTAAG ACGCATGGTT 
TTGGTCCTTA CTCAGCAAAA CAATGAGAGC AAAGAGTTTG TAAAGTTCTT TCATAAGCAG 
CTTGGAAGTA TATTACAGGA TTCACTCGCA AAATTTGCTG GCAGAAAACT GAAGGATTGT 
GGAGAAGATC TTCTTGTAGA GATATCTGAA GTGTTATTTA ATGAATTGGC TTTCTTTAAG 
CTCATGCAAG ATTTGGATAA TAATAGTGTA ACTGTTAAAC AGAGATGCAA AAGGAAAATA 
GAAGCTGCTG GAGTGATACA GTCTTATGCA AAAGAGGCCA AAAGGATTCT TGAAGGAGAT 
CACAGTTCAC CTGCTGGAGA AATTGATGAT GAAGACAAAG ACAAGGATGA GACGGAAACA 
GTTAAGCAGA CTCAAACGTC TGAGGTTTAT GATGGTGACG GCCCCAAAAA TGTGAGATCT 
GATGTTTCTG ATCAAGAGGA TGATGAAGAA AGTGAAGAAT GTCCAGTGTC TATTAATTTG 
TCCAAAGCTG AAACTCAGGC TTTGACTAAT TACGGAAGTG GAGAAGATGA GAATGAAGAT 
GAAGAAATAG AAGAATTTGA AGAGGGACCT GTAGATGTCC AGACGTCACT TCAGGCTAAC 
ACTGAAACTA CAGAAGAAAA TGAACACGAT GATCAGGTTC CACAGCATGA TTTTGAAAAA 
TCATCAGAAA GCAAAAATGT CCCATCAGAA CAAGAACCCA CTACTAGTAA AGACTTTCTT 
TTGATGACAG GTGACCAGAA TAGCACTCCT GTGAAGCCCT GTTACCTCAG TATCGTGGAA 
AATGAGCAAC CTTTAAATAG CCCTGTCCAG AAGGACACTC TCACTACCAT CGACTCATCC 
AGTCAGCCTA ACCCTTTGCC TTTACCTTTA ACTGAAATTG AAACCTTGGT GCCTAGAGTC 
AAAGAAGTGA AATCTGCTCA GGAGACTCCT GAAAGTTCTT TGGCTGGAAG TCCTGATACT 
GAATCTCCAG TGTTAGTGAA TGACTATGAA GCAGAATCTG GTAACATAAG TCAAAAATCT 
GATGAAGAAG ACTTTGTGAA AGTTGAAGAT TTACCACTTA AACTGACAAT ATATTCAGAG 
GCAGATCTAA GGAAGAAAAT GGCCGAAGAA GAACAGAAAA ACCACTTATC TGGTGAAATA 
TGTGCAATGC AGACTGAAGA ATTAGCTGGG AATTCTCAGA CACTAAAAGA ACCTGAGCAA 
GTATGTGCTC AGTCTGCCAT CTTAAGATGG AAACCCTTAC CTGTGCCTTT TAAACTGTTG 
GTGGTGTCAT CAGGGAATTA TTGTAGCAAA AAACAGTGGG AGCCCAAAGT ACATGAGATG 
TCTCCAGAGG CTCAGCTAAC TCTTTCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025135214.1
CDS	392..7021
Translation	MATGGGPFEEGVNDQDLPNWSNESVDDRLNNMDWGSQQKKANRSSEKNKRKFGVESDKRVTNDISPESSPGVGRRRTKTSLTFPHSRYMTQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDATVSPQKREMIGSAQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIREYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLQKILARENEEEDVRTIDSAVGSGSVAESTSLNIDVRSEASDATEASFSLRIRPCVEDKLGNSASQAQVSDTDVTTSPKGKGDRPPQHDRELRPNRKCSRKRGFPSKARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSEKVAETTPGHHTVPGRQPAHSPLHQRVPIRETTGSLSGVSITSELNEELNDLIQHFHNQLRDSQPPAVPDNRRQAESLSLTREISQSRNPSVSEHLPDEKVQLFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSFVPSSASPQRSVDQRSTTTAPSAPVVLTPVVNGESNNLTPSVPYPAASLVSQNQNENEGHLNPTEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENTKEEETEESEYDSEHENSEPVTNIRNPQVAATWNEVNSNSNAQCVSNNRDGRAVNSNCEINNRSAANIRALNMPPLSADCRYNREGEQEMPVAQGHEEEEEEEEEAEDEALSGASLSSHSSSLIIETPEDAEFEHKIQKLMAAKLKLRHLQNLVAMVQDDDAANQGVTSVEDFFPAEDTKQNANNTRGNANKTQKDAGVNEKAREKFYEAKLQQQQRELKQLQEERKKLIEIQEKIQELQKACPNLQLSAASVGNCPTKTHMPAVTSSPTVNENEASTNKSGFEPHDSSVVDNEVWSDMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETASPVAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGIIRTDEEEEEEEQDASSNDNFHMYPPNSANHNSYNVKETKNRWKNNRPFSADGNYRPLAKTRQQNISMQRQENLRWVSELSYVEEKEQWQEQINQLKKQLDFSVNICQTLMQDQQTLSCLLQTLLTSPYSVMPSSVASPQVHLIMHQLNQCYTQLTWQQNNVQRLKQMLSELMRQQNQHPEKPASKEGGSGTSHPPSPSLFCPFNFPTQPVNLFNLPGFTNFSSFAPSMNFSPLFPSNFGDFSQNISTPTEQQQPLAQNPSGKTEYMAFPKPFESSSSIGAEKQRNQKQPEEEVENSRTPWFYDQEGEVEKPFLKTGFAVSVEKTTNSNRKNQLDTSRRRRQFDEVSLESFNSMLDPVDPTTVTKTFKSRKASAQASLASKDKTPKSKSKKRHSTQLKSRVKNIGYESASVSSTCEPCKSRNRHSAQTEEPVQAKVLSRKNHEQLEKVIRCSRSTEISSETGSDFSMFEALRDTIYSEVATLISQNESRPHFLIELFHELQLLNTDYLRQRALYALQDIVSRHISENHEKEGENIKSVNSGTWIASNSELTPSESLATTDDETFEKNFERETHKISEQNDADNASVSSTCEPFATDVLGDTVIHLDQALARMRDYTHMKMEAESSPDVRCTCRIIEDDDGAAATTTISNNLEETPVIENHSSQQPVSEVATVPCPRIDTQQLDRQIKAIMKEVIPFLKEHMDEVCSSQLLTSVRRMVLVLTQQNNESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSVTVKQRCKRKIEAAGVIQSYAKEAKRILEGDHSSPAGEIDDEDKDKDETETVKQTQTSEVYDGDGPKNVRSDVSDQEDDEESEECPVSINLSKAETQALTNYGSGEDENEDEEIEEFEEGPVDVQTSLQANTETTEENEHDDQVPQHDFEKSSESKNVPSEQEPTTSKDFLLMTGDQNSTPVKPCYLSIVENEQPLNSPVQKDTLTTIDSSSQPNPLPLPLTEIETLVPRVKEVKSAQETPESSLAGSPDTESPVLVNDYEAESGNISQKSDEEDFVKVEDLPLKLTIYSEADLRKKMAEEEQKNHLSGEICAMQTEELAGNSQTLKEPEQVCAQSAILRWKPLPVPFKLLVVSSGNYCSKKQWEPKVHEMSPEAQLTLS

Target ORF information:

RefSeq Version	XM_025279429.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis pericentriolar material 1 (PCM1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025279429.1

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
AGCAATGAGA GTGTTGATGA CCGACTCAAC AATATGGATT GGGGCAGCCA GCAGAAGAAG 
GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA 
ACTAACGATA TTTCTCCGGA GTCATCACCG GGAGTTGGAA GACGAAGAAC AAAGACTTCA 
CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TCCCCGAGCA GGCAGAACTA 
GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCTCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCCAC TGTAAGTCCT 
CAAAAGAGAG AAATGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAGATTCGTG AGTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGACCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTCACT 
CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT CCAGAAGATT 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCGGT CTGAGGCTTC AGATGCCACG 
GAGGCATCTT TTAGTCTGAG AATTCGGCCG TGCGTTGAGG ACAAACTAGG GAATTCAGCT 
TCACAAGCAC AGGTTTCAGA CACTGACGTT ACTACAAGTC CAAAAGGGAA AGGTGACAGA 
CCTCCTCAGC ATGACAGGGA GCTGAGGCCT AATAGGAAAT GTAGCCGAAA ACGTGGATTT 
CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATGGAAG AGATAGAAAA TTTGAAGAAA 
CAACATGATT TATTAAAAAG GATGTTACAG CAGCAGGAGC AGCTGAGAGC TCTACAAGGA 
CGACAAGCTG CTCTGCTTGC CCTGCAGCAT AAAGCTGAGC AGGCTATAGC TGTGATGGAT 
GATTCTGAAA AGGTTGCGGA GACAACTCCT GGCCATCATA CTGTACCAGG CAGACAGCCG 
GCTCATTCAC CTTTGCATCA GAGAGTACCC ATAAGAGAAA CTACAGGTAG CCTATCTGGA 
GTCAGTATCA CCTCTGAACT AAACGAAGAA TTGAATGATT TAATTCAGCA TTTTCATAAT 
CAGCTTCGTG ATTCTCAGCC TCCAGCTGTT CCAGACAATA GAAGACAAGC AGAAAGTCTT 
TCATTAACTA GGGAGATTTC TCAGAGCAGA AATCCTTCAG TTTCAGAGCA TTTACCTGAT 
GAGAAAGTCC AACTTTTTAG CAAGATGAGA GTGTTACAAG AAAAGAAACA AAAAATGGAC 
AAACTTCTTG GAGAACTTCA TACACTTCGA GATCAGCATT TGAACAATTC ATCATTTGTG 
CCTTCTTCAG CCTCTCCACA AAGGAGTGTG GATCAGAGAA GTACAACCAC AGCTCCTTCT 
GCTCCTGTAG TCTTAACACC AGTGGTCAAT GGAGAATCCA ATAACCTCAC GCCATCTGTT 
CCTTATCCTG CTGCTTCTCT AGTTTCTCAG AATCAGAATG AAAATGAAGG CCACCTAAAT 
CCAACTGAAA AACTCCAGAA GTTAAATGAA GTTCGAAAGA GGTTGAATGA ACTAAGAGAA 
TTAGTTCATT ATTATGAGCA AACATCAGAT ATGATGACAG ATGCTGTGAA TGAAAACACA 
AAAGAAGAAG AAACTGAAGA ATCAGAATAT GATTCGGAGC ATGAAAACTC GGAACCTGTT 
ACTAACATTC GAAATCCACA AGTGGCTGCC ACCTGGAACG AAGTAAATAG TAATTCTAAT 
GCACAGTGTG TTTCCAACAA TAGAGATGGG CGAGCAGTTA ATTCTAACTG TGAAATTAAC 
AACAGGTCTG CTGCCAACAT AAGGGCTCTA AACATGCCTC CTCTTTCAGC AGATTGTCGA 
TACAATAGAG AAGGAGAACA GGAGATGCCT GTTGCACAAG GTCATGAGGA GGAGGAGGAG 
GAGGAGGAGG AAGCAGAAGA TGAGGCCCTC AGTGGAGCTT CACTGTCTAG TCACAGCAGC 
AGCCTGATCA TTGAGACTCC TGAAGATGCT GAATTTGAAC ATAAGATCCA GAAGCTTATG 
GCTGCAAAAC TGAAACTTAG ACACTTACAG AACCTTGTAG CCATGGTGCA GGACGATGAT 
GCAGCCAATC AAGGAGTTAC TTCTGTGGAG GATTTCTTCC CAGCAGAAGA CACCAAACAA 
AATGCAAATA ACACTAGAGG AAATGCCAAT AAAACACAGA AAGATGCTGG AGTAAATGAA 
AAGGCAAGGG AGAAATTTTA TGAGGCTAAA CTACAGCAGC AACAGAGAGA ACTCAAACAA 
TTGCAGGAAG AAAGAAAGAA ACTGATTGAG ATTCAAGAGA AAATTCAAGA ATTGCAAAAG 
GCATGTCCTA ACCTACAGCT GTCAGCTGCT AGTGTGGGTA ATTGTCCCAC AAAAACACAT 
ATGCCAGCTG TTACTTCATC CCCAACCGTT AATGAAAATG AAGCCAGTAC AAACAAATCT 
GGTTTTGAGC CTCATGATTC TTCAGTGGTA GATAATGAGG TATGGTCAGA TATGCGAAGA 
CATGAAATGT TAAGGGAGGA GCTACGACAG AGAAGAAAGC AGCTGGAAGC TCTGATGGCT 
GAACATCAGA GGAGGCAAGG TCTGGCTGAA ACTGCATCTC CAGTGGCTGT TTCACTGAGA 
AGTGATGGAT CTGAGAACCT GTGTACACCT CAGCAAAGCA GAACAGAAAA AACAATGGCA 
ACTTGGGGAG GTTCTACCCA GTGTGCCCTA GATGAAGAAG GAGATGAAGA TGGTTACCTT 
TCTGAAGGAA TTATTCGGAC AGACGAAGAG GAGGAAGAGG AGGAGCAAGA TGCCAGTTCC 
AATGATAACT TTCATATGTA TCCTCCAAAC AGTGCAAATC ATAATTCTTA CAATGTAAAG 
GAAACTAAAA ATAGGTGGAA GAACAATCGC CCTTTTTCAG CAGATGGAAA TTATCGTCCT 
TTAGCCAAGA CAAGACAGCA GAATATCAGC ATGCAGCGTC AGGAAAACCT TCGCTGGGTG 
TCCGAACTCT CTTATGTAGA AGAGAAAGAA CAATGGCAAG AACAAATTAA TCAGCTAAAG 
AAACAACTTG ATTTCAGTGT CAATATTTGT CAGACTTTAA TGCAAGACCA GCAGACTCTG 
TCTTGTCTGC TACAAACTCT TCTCACGAGT CCTTATAGTG TTATGCCCAG CAGTGTTGCA 
TCCCCGCAAG TGCACCTTAT AATGCACCAG TTGAATCAGT GCTATACCCA GCTAACGTGG 
CAGCAGAACA ATGTCCAGAG GTTGAAACAA ATGCTCAGTG AACTCATGCG CCAACAAAAT 
CAGCATCCAG AAAAACCTGC AAGCAAGGAA GGAGGCAGTG GTACATCACA CCCTCCTTCT 
CCCAGTTTAT TTTGTCCTTT CAACTTTCCA ACTCAGCCTG TAAATCTGTT TAACCTACCG 
GGGTTTACTA ATTTTTCGTC ATTTGCACCA AGTATGAATT TCAGCCCTTT ATTTCCTTCT 
AATTTTGGAG ATTTTTCTCA AAATATTTCT ACACCCACTG AACAGCAGCA ACCGTTAGCC 
CAGAATCCTT CAGGGAAAAC GGAATACATG GCTTTTCCAA AACCTTTTGA AAGTAGTTCT 
TCTATTGGAG CGGAAAAACA AAGGAATCAA AAACAGCCTG AAGAGGAGGT GGAGAACAGT 
AGGACACCAT GGTTTTATGA CCAAGAAGGT GAAGTAGAAA AGCCATTTCT CAAGACTGGA 
TTTGCAGTGT CTGTAGAGAA AACAACAAAT AGTAATCGCA AAAATCAACT AGATACCAGT 
AGGAGAAGAC GCCAGTTTGA TGAAGTGTCC TTAGAAAGCT TTAACAGTAT GCTTGACCCA 
GTAGATCCAA CAACAGTAAC TAAAACATTC AAGTCAAGAA AAGCATCTGC ACAGGCCAGC 
CTGGCATCTA AAGATAAAAC TCCCAAATCA AAGAGTAAGA AGAGGCATTC TACTCAGCTG 
AAAAGCAGAG TGAAAAATAT CGGGTATGAA AGTGCCAGTG TGTCTAGCAC ATGTGAACCT 
TGCAAAAGTA GAAACAGACA TTCAGCCCAA ACTGAAGAGC CTGTTCAAGC AAAAGTACTC 
AGCAGAAAGA ATCATGAGCA GCTGGAAAAA GTCATAAGAT GTAGTAGGTC TACAGAAATA 
TCTTCAGAAA CCGGGAGTGA TTTTTCCATG TTCGAAGCTT TGCGGGATAC CATTTATTCT 
GAAGTAGCTA CGTTGATTTC TCAAAATGAA TCTCGTCCAC ATTTTCTTAT TGAACTCTTC 
CATGAGCTTC AGCTACTTAA TACAGACTAT TTGAGACAGA GGGCCTTATA TGCATTGCAG 
GACATAGTAT CCAGGCATAT TTCTGAGAAC CATGAAAAAG AAGGAGAAAA TATAAAGTCC 
GTGAATTCTG GTACTTGGAT AGCATCCAAC TCAGAACTTA CTCCCAGTGA AAGCCTTGCT 
ACTACTGATG ATGAAACTTT TGAGAAGAAC TTTGAGAGAG AAACACATAA AATAAGTGAG 
CAAAATGATG CTGATAATGC TAGTGTATCT TCAACTTGTG AGCCTTTTGC AACAGATGTT 
CTGGGCGACA CTGTGATTCA CTTAGATCAG GCATTAGCCA GAATGAGAGA CTATACGCAT 
ATGAAGATGG AGGCTGAAAG TAGTCCAGAC GTCAGGTGCA CCTGCAGGAT CATTGAGGAT 
GACGATGGTG CTGCTGCCAC AACCACGATT AGTAATAATT TAGAAGAAAC TCCTGTTATT 
GAAAATCATA GTTCACAGCA ACCCGTGAGT GAAGTTGCTA CCGTCCCGTG TCCTAGAATT 
GATACTCAGC AACTGGACCG GCAAATTAAA GCAATTATGA AAGAAGTCAT TCCTTTTTTG 
AAGGAGCACA TGGATGAAGT ATGTTCTTCT CAACTTCTAA CCTCAGTAAG ACGCATGGTT 
TTGGTCCTTA CTCAGCAAAA CAATGAGAGC AAAGAGTTTG TAAAGTTCTT TCATAAGCAG 
CTTGGAAGTA TATTACAGGA TTCACTCGCA AAATTTGCTG GCAGAAAACT GAAGGATTGT 
GGAGAAGATC TTCTTGTAGA GATATCTGAA GTGTTATTTA ATGAATTGGC TTTCTTTAAG 
CTCATGCAAG ATTTGGATAA TAATAGTGTA ACTGTTAAAC AGAGATGCAA AAGGAAAATA 
GAAGCTGCTG GAGTGATACA GTCTTATGCA AAAGAGGCCA AAAGGATTCT TGAAGGAGAT 
CACAGTTCAC CTGCTGGAGA AATTGATGAT GAAGACAAAG ACAAGGATGA GACGGAAACA 
GTTAAGCAGA CTCAAACGTC TGAGGTTTAT GATGGTGACG GCCCCAAAAA TGTGAGATCT 
GATGTTTCTG ATCAAGAGGA TGATGAAGAA AGTGAAGAAT GTCCAGTGTC TATTAATTTG 
TCCAAAGCTG AAACTCAGGC TTTGACTAAT TACGGAAGTG GAGAAGATGA GAATGAAGAT 
GAAGAAATAG AAGAATTTGA AGAGGGACCT GTAGATGTCC AGACGTCACT TCAGGCTAAC 
ACTGAAACTA CAGAAGAAAA TGAACACGAT GATCAGGTTC CACAGCATGA TTTTGAAAAA 
TCATCAGAAA GCAAAAATGT CCCATCAGAA CAAGAACCCA CTACTAGTAA AGACTTTCTT 
TTGATGACAG GTGACCAGAA TAGCACTCCT GTGAAGCCCT GTTACCTCAG TATCGTGGAA 
AATGAGCAAC CTTTAAATAG CCCTGTCCAG AAGGACACTC TCACTACCAT CGACTCATCC 
AGTCAGCCTA ACCCTTTGCC TTTACCTTTA ACTGAAATTG AAACCTTGGT GCCTAGAGTC 
AAAGAAGTGA AATCTGCTCA GGAGACTCCT GAAAGTTCTT TGGCTGGAAG TCCTGATACT 
GAATCTCCAG TGTTAGTGAA TGACTATGAA GCAGAATCTG GTAACATAAG TCAAAAATCT 
GATGAAGAAG ACTTTGTGAA AGTTGAAGAT TTACCACTTA AACTGACAAT ATATTCAGAG 
GCAGATCTAA GGAAGAAAAT GGCCGAAGAA GAACAGAAAA ACCACTTATC TGGTGAAATA 
TGTGCAATGC AGACTGAAGA ATTAGCTGGG AATTCTCAGA CACTAAAAGA ACCTGAGCAA 
GTATGTGCTC AGTCTGCCAT CTTAAGATGG AAACCCTTAC CTGTGCCTTT TAAACTGTTG 
GTGGTGTCAT CAGGGAATTA TTGTAGCAAA AAACAGTGGG AGCCCAAAGT ACATGAGATG 
TCTCCAGAGG CTCAGCTAAC TCTTTCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( XM_025279440.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025135225.1 Bubalus bubalis pericentriolar material 1 (PCM1), transcript variant X6, mRNA.

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CloneID	OBu42294
Clone ID Related Accession (Same CDS sequence)	XM_025279440.1
Accession Version	XM_025279440.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6627bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	pericentriolar material 1 protein isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037545.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG GAGGAGGTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
AGCAATGAGA GTGTTGATGA CCGACTCAAC AATATGGATT GGGGCAGCCA GCAGAAGAAG 
GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA 
ACTAACGATA TTTCTCCGGA GTCATCACCG GGAGTTGGAA GACGAAGAAC AAAGACTTCA 
CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TCCCCGAGCA GGCAGAACTA 
GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCTCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCCAC TGTAAGTCCT 
CAAAAGAGAG AAATGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAGATTCGTG AGTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGACCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTCACT 
CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT CCAGAAGATT 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCGGT CTGAGGCTTC AGATGCCACG 
GAGGCATCTT TTAGTCTGAG AATTCGGCCG TGCGTTGAGG ACAAACTAGG GAATTCAGCT 
TCACAAGCAC AGGTTTCAGA CACTGACGTT ACTACAAGTC CAAAAGGGAA AGGTGACAGA 
CCTCCTCAGC ATGACAGGGA GCTGAGGCCT AATAGGAAAT GTAGCCGAAA ACGTGGATTT 
CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATGGAAG AGATAGAAAA TTTGAAGAAA 
CAACATGATT TATTAAAAAG GATGTTACAG CAGCAGGAGC AGCTGAGAGC TCTACAAGGA 
CGACAAGCTG CTCTGCTTGC CCTGCAGCAT AAAGCTGAGC AGGCTATAGC TGTGATGGAT 
GATTCTGAAA AGGTTGCGGA GACAACTCCT GGCCATCATA CTGTACCAGG CAGACAGCCG 
GCTCATTCAC CTTTGCATCA GAGAGTACCC ATAAGAGAAA CTACAGGTAG CCTATCTGGA 
GTCAGTATCA CCTCTGAACT AAACGAAGAA TTGAATGATT TAATTCAGCA TTTTCATAAT 
CAGCTTCGTG ATTCTCAGCC TCCAGCTGTT CCAGACAATA GAAGACAAGC AGAAAGTCTT 
TCATTAACTA GGGAGATTTC TCAGAGCAGA AATCCTTCAG TTTCAGAGCA TTTACCTGAT 
GAGAAAGTCC AACTTTTTAG CAAGATGAGA GTGTTACAAG AAAAGAAACA AAAAATGGAC 
AAACTTCTTG GAGAACTTCA TACACTTCGA GATCAGCATT TGAACAATTC ATCATTTGTG 
CCTTCTTCAG CCTCTCCACA AAGGAGTGTG GATCAGAGAA GTACAACCAC AGCTCCTTCT 
GCTCCTGTAG TCTTAACACC AGTGGTCAAT GGAGAATCCA ATAACCTCAC GCCATCTGTT 
CCTTATCCTG CTGCTTCTCT AGTTTCTCAG AATCAGAATG AAAATGAAGG CCACCTAAAT 
CCAACTGAAA AACTCCAGAA GTTAAATGAA GTTCGAAAGA GGTTGAATGA ACTAAGAGAA 
TTAGTTCATT ATTATGAGCA AACATCAGAT ATGATGACAG ATGCTGTGAA TGAAAACACA 
AAAGAAGAAG AAACTGAAGA ATCAGAATAT GATTCGGAGC ATGAAAACTC GGAACCTGTT 
ACTAACATTC GAAATCCACA AGTGGCTGCC ACCTGGAACG AAGTAAATAG TAATTCTAAT 
GCACAGTGTG TTTCCAACAA TAGAGATGGG CGAGCAGTTA ATTCTAACTG TGAAATTAAC 
AACAGGTCTG CTGCCAACAT AAGGGCTCTA AACATGCCTC CTCTTTCAGA TTGTCGATAC 
AATAGAGAAG GAGAACAGGA GATGCCTGTT GCACAAGGTC ATGAGGAGGA GGAGGAGGAG 
GAGGAGGAAG CAGAAGATGA GGCCCTCAGT GGAGCTTCAC TGTCTAGTCA CAGCAGCAGC 
CTGATCATTG AGACTCCTGA AGATGCTGAA TTTGAACATA AGATCCAGAA GCTTATGGCT 
GCAAAACTGA AACTTAGACA CTTACAGAAC CTTGTAGCCA TGGTGCAGGA CGATGATGCA 
GCCAATCAAG GAGTTACTTC TGTGGAGGAT TTCTTCCCAG CAGAAGACAC CAAACAAAAT 
GCAAATAACA CTAGAGGAAA TGCCAATAAA ACACAGAAAG ATGCTGGAGT AAATGAAAAG 
GCAAGGGAGA AATTTTATGA GGCTAAACTA CAGCAGCAAC AGAGAGAACT CAAACAATTG 
CAGGAAGAAA GAAAGAAACT GATTGAGATT CAAGAGAAAA TTCAAGAATT GCAAAAGGCA 
TGTCCTAACC TACAGCTGTC AGCTGCTAGT GTGGGTAATT GTCCCACAAA AACACATATG 
CCAGCTGTTA CTTCATCCCC AACCGTTAAT GAAAATGAAG CCAGTACAAA CAAATCTGGT 
TTTGAGCCTC ATGATTCTTC AGTGGTAGAT AATGAGGTAT GGTCAGATAT GCGAAGACAT 
GAAATGTTAA GGGAGGAGCT ACGACAGAGA AGAAAGCAGC TGGAAGCTCT GATGGCTGAA 
CATCAGAGGA GGCAAGGTCT GGCTGAAACT GCATCTCCAG TGGCTGTTTC ACTGAGAAGT 
GATGGATCTG AGAACCTGTG TACACCTCAG CAAAGCAGAA CAGAAAAAAC AATGGCAACT 
TGGGGAGGTT CTACCCAGTG TGCCCTAGAT GAAGAAGGAG ATGAAGATGG TTACCTTTCT 
GAAGGAATTA TTCGGACAGA CGAAGAGGAG GAAGAGGAGG AGCAAGATGC CAGTTCCAAT 
GATAACTTTC ATATGTATCC TCCAAACAGT GCAAATCATA ATTCTTACAA TGTAAAGGAA 
ACTAAAAATA GGTGGAAGAA CAATCGCCCT TTTTCAGCAG ATGGAAATTA TCGTCCTTTA 
GCCAAGACAA GACAGCAGAA TATCAGCATG CAGCGTCAGG AAAACCTTCG CTGGGTGTCC 
GAACTCTCTT ATGTAGAAGA GAAAGAACAA TGGCAAGAAC AAATTAATCA GCTAAAGAAA 
CAACTTGATT TCAGTGTCAA TATTTGTCAG ACTTTAATGC AAGACCAGCA GACTCTGTCT 
TGTCTGCTAC AAACTCTTCT CACGAGTCCT TATAGTGTTA TGCCCAGCAG TGTTGCATCC 
CCGCAAGTGC ACCTTATAAT GCACCAGTTG AATCAGTGCT ATACCCAGCT AACGTGGCAG 
CAGAACAATG TCCAGAGGTT GAAACAAATG CTCAGTGAAC TCATGCGCCA ACAAAATCAG 
CATCCAGAAA AACCTGCAAG CAAGGAAGGA GGCAGTGGTA CATCACACCC TCCTTCTCCC 
AGTTTATTTT GTCCTTTCAA CTTTCCAACT CAGCCTGTAA ATCTGTTTAA CCTACCGGGG 
TTTACTAATT TTTCGTCATT TGCACCAAGT ATGAATTTCA GCCCTTTATT TCCTTCTAAT 
TTTGGAGATT TTTCTCAAAA TATTTCTACA CCCACTGAAC AGCAGCAACC GTTAGCCCAG 
AATCCTTCAG GGAAAACGGA ATACATGGCT TTTCCAAAAC CTTTTGAAAG TAGTTCTTCT 
ATTGGAGCGG AAAAACAAAG GAATCAAAAA CAGCCTGAAG AGGAGGTGGA GAACAGTAGG 
ACACCATGGT TTTATGACCA AGAAGGTGAA GTAGAAAAGC CATTTCTCAA GACTGGATTT 
GCAGTGTCTG TAGAGAAAAC AACAAATAGT AATCGCAAAA ATCAACTAGA TACCAGTAGG 
AGAAGACGCC AGTTTGATGA AGTGTCCTTA GAAAGCTTTA ACAGTATGCT TGACCCAGTA 
GATCCAACAA CAGTAACTAA AACATTCAAG TCAAGAAAAG CATCTGCACA GGCCAGCCTG 
GCATCTAAAG ATAAAACTCC CAAATCAAAG AGTAAGAAGA GGCATTCTAC TCAGCTGAAA 
AGCAGAGTGA AAAATATCGG GTATGAAAGT GCCAGTGTGT CTAGCACATG TGAACCTTGC 
AAAAGTAGAA ACAGACATTC AGCCCAAACT GAAGAGCCTG TTCAAGCAAA AGTACTCAGC 
AGAAAGAATC ATGAGCAGCT GGAAAAAGTC ATAAGATGTA GTAGGTCTAC AGAAATATCT 
TCAGAAACCG GGAGTGATTT TTCCATGTTC GAAGCTTTGC GGGATACCAT TTATTCTGAA 
GTAGCTACGT TGATTTCTCA AAATGAATCT CGTCCACATT TTCTTATTGA ACTCTTCCAT 
GAGCTTCAGC TACTTAATAC AGACTATTTG AGACAGAGGG CCTTATATGC ATTGCAGGAC 
ATAGTATCCA GGCATATTTC TGAGAACCAT GAAAAAGAAG GAGAAAATAT AAAGTCCGTG 
AATTCTGGTA CTTGGATAGC ATCCAACTCA GAACTTACTC CCAGTGAAAG CCTTGCTACT 
ACTGATGATG AAACTTTTGA GAAGAACTTT GAGAGAGAAA CACATAAAAT AAGTGAGCAA 
AATGATGCTG ATAATGCTAG TGTATCTTCA ACTTGTGAGC CTTTTGCAAC AGATGTTCTG 
GGCGACACTG TGATTCACTT AGATCAGGCA TTAGCCAGAA TGAGAGACTA TACGCATATG 
AAGATGGAGG CTGAAAGTAG TCCAGACGTC AGGTGCACCT GCAGGATCAT TGAGGATGAC 
GATGGTGCTG CTGCCACAAC CACGATTAGT AATAATTTAG AAGAAACTCC TGTTATTGAA 
AATCATAGTT CACAGCAACC CGTGAGTGAA GTTGCTACCG TCCCGTGTCC TAGAATTGAT 
ACTCAGCAAC TGGACCGGCA AATTAAAGCA ATTATGAAAG AAGTCATTCC TTTTTTGAAG 
GAGCACATGG ATGAAGTATG TTCTTCTCAA CTTCTAACCT CAGTAAGACG CATGGTTTTG 
GTCCTTACTC AGCAAAACAA TGAGAGCAAA GAGTTTGTAA AGTTCTTTCA TAAGCAGCTT 
GGAAGTATAT TACAGGATTC ACTCGCAAAA TTTGCTGGCA GAAAACTGAA GGATTGTGGA 
GAAGATCTTC TTGTAGAGAT ATCTGAAGTG TTATTTAATG AATTGGCTTT CTTTAAGCTC 
ATGCAAGATT TGGATAATAA TAGTGTAACT GTTAAACAGA GATGCAAAAG GAAAATAGAA 
GCTGCTGGAG TGATACAGTC TTATGCAAAA GAGGCCAAAA GGATTCTTGA AGGAGATCAC 
AGTTCACCTG CTGGAGAAAT TGATGATGAA GACAAAGACA AGGATGAGAC GGAAACAGTT 
AAGCAGACTC AAACGTCTGA GGTTTATGAT GGTGACGGCC CCAAAAATGT GAGATCTGAT 
GTTTCTGATC AAGAGGATGA TGAAGAAAGT GAAGAATGTC CAGTGTCTAT TAATTTGTCC 
AAAGCTGAAA CTCAGGCTTT GACTAATTAC GGAAGTGGAG AAGATGAGAA TGAAGATGAA 
GAAATAGAAG AATTTGAAGA GGGACCTGTA GATGTCCAGA CGTCACTTCA GGCTAACACT 
GAAACTACAG AAGAAAATGA ACACGATGAT CAGGTTCCAC AGCATGATTT TGAAAAATCA 
TCAGAAAGCA AAAATGTCCC ATCAGAACAA GAACCCACTA CTAGTAAAGA CTTTCTTTTG 
ATGACAGGTG ACCAGAATAG CACTCCTGTG AAGCCCTGTT ACCTCAGTAT CGTGGAAAAT 
GAGCAACCTT TAAATAGCCC TGTCCAGAAG GACACTCTCA CTACCATCGA CTCATCCAGT 
CAGCCTAACC CTTTGCCTTT ACCTTTAACT GAAATTGAAA CCTTGGTGCC TAGAGTCAAA 
GAAGTGAAAT CTGCTCAGGA GACTCCTGAA AGTTCTTTGG CTGGAAGTCC TGATACTGAA 
TCTCCAGTGT TAGTGAATGA CTATGAAGCA GAATCTGGTA ACATAAGTCA AAAATCTGAT 
GAAGAAGACT TTGTGAAAGT TGAAGATTTA CCACTTAAAC TGACAATATA TTCAGAGGCA 
GATCTAAGGA AGAAAATGGC CGAAGAAGAA CAGAAAAACC ACTTATCTGG TGAAATATGT 
GCAATGCAGA CTGAAGAATT AGCTGGGAAT TCTCAGACAC TAAAAGAACC TGAGCAAGTA 
TGTGCTCAGT CTGCCATCTT AAGATGGAAA CCCTTACCTG TGCCTTTTAA ACTGTTGGTG 
GTGTCATCAG GGAATTATTG TAGCAAAAAA CAGTGGGAGC CCAAAGTACA TGAGATGTCT 
CCAGAGGCTC AGCTAACTCT TTCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025135225.1
CDS	392..7018
Translation	MATGGGPFEEGVNDQDLPNWSNESVDDRLNNMDWGSQQKKANRSSEKNKRKFGVESDKRVTNDISPESSPGVGRRRTKTSLTFPHSRYMTQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDATVSPQKREMIGSAQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIREYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLQKILARENEEEDVRTIDSAVGSGSVAESTSLNIDVRSEASDATEASFSLRIRPCVEDKLGNSASQAQVSDTDVTTSPKGKGDRPPQHDRELRPNRKCSRKRGFPSKARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSEKVAETTPGHHTVPGRQPAHSPLHQRVPIRETTGSLSGVSITSELNEELNDLIQHFHNQLRDSQPPAVPDNRRQAESLSLTREISQSRNPSVSEHLPDEKVQLFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSFVPSSASPQRSVDQRSTTTAPSAPVVLTPVVNGESNNLTPSVPYPAASLVSQNQNENEGHLNPTEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENTKEEETEESEYDSEHENSEPVTNIRNPQVAATWNEVNSNSNAQCVSNNRDGRAVNSNCEINNRSAANIRALNMPPLSDCRYNREGEQEMPVAQGHEEEEEEEEEAEDEALSGASLSSHSSSLIIETPEDAEFEHKIQKLMAAKLKLRHLQNLVAMVQDDDAANQGVTSVEDFFPAEDTKQNANNTRGNANKTQKDAGVNEKAREKFYEAKLQQQQRELKQLQEERKKLIEIQEKIQELQKACPNLQLSAASVGNCPTKTHMPAVTSSPTVNENEASTNKSGFEPHDSSVVDNEVWSDMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETASPVAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGIIRTDEEEEEEEQDASSNDNFHMYPPNSANHNSYNVKETKNRWKNNRPFSADGNYRPLAKTRQQNISMQRQENLRWVSELSYVEEKEQWQEQINQLKKQLDFSVNICQTLMQDQQTLSCLLQTLLTSPYSVMPSSVASPQVHLIMHQLNQCYTQLTWQQNNVQRLKQMLSELMRQQNQHPEKPASKEGGSGTSHPPSPSLFCPFNFPTQPVNLFNLPGFTNFSSFAPSMNFSPLFPSNFGDFSQNISTPTEQQQPLAQNPSGKTEYMAFPKPFESSSSIGAEKQRNQKQPEEEVENSRTPWFYDQEGEVEKPFLKTGFAVSVEKTTNSNRKNQLDTSRRRRQFDEVSLESFNSMLDPVDPTTVTKTFKSRKASAQASLASKDKTPKSKSKKRHSTQLKSRVKNIGYESASVSSTCEPCKSRNRHSAQTEEPVQAKVLSRKNHEQLEKVIRCSRSTEISSETGSDFSMFEALRDTIYSEVATLISQNESRPHFLIELFHELQLLNTDYLRQRALYALQDIVSRHISENHEKEGENIKSVNSGTWIASNSELTPSESLATTDDETFEKNFERETHKISEQNDADNASVSSTCEPFATDVLGDTVIHLDQALARMRDYTHMKMEAESSPDVRCTCRIIEDDDGAAATTTISNNLEETPVIENHSSQQPVSEVATVPCPRIDTQQLDRQIKAIMKEVIPFLKEHMDEVCSSQLLTSVRRMVLVLTQQNNESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSVTVKQRCKRKIEAAGVIQSYAKEAKRILEGDHSSPAGEIDDEDKDKDETETVKQTQTSEVYDGDGPKNVRSDVSDQEDDEESEECPVSINLSKAETQALTNYGSGEDENEDEEIEEFEEGPVDVQTSLQANTETTEENEHDDQVPQHDFEKSSESKNVPSEQEPTTSKDFLLMTGDQNSTPVKPCYLSIVENEQPLNSPVQKDTLTTIDSSSQPNPLPLPLTEIETLVPRVKEVKSAQETPESSLAGSPDTESPVLVNDYEAESGNISQKSDEEDFVKVEDLPLKLTIYSEADLRKKMAEEEQKNHLSGEICAMQTEELAGNSQTLKEPEQVCAQSAILRWKPLPVPFKLLVVSSGNYCSKKQWEPKVHEMSPEAQLTLS

Target ORF information:

RefSeq Version	XM_025279440.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis pericentriolar material 1 (PCM1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025279440.1

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
AGCAATGAGA GTGTTGATGA CCGACTCAAC AATATGGATT GGGGCAGCCA GCAGAAGAAG 
GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA 
ACTAACGATA TTTCTCCGGA GTCATCACCG GGAGTTGGAA GACGAAGAAC AAAGACTTCA 
CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TCCCCGAGCA GGCAGAACTA 
GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCTCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCCAC TGTAAGTCCT 
CAAAAGAGAG AAATGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAGATTCGTG AGTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGACCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTCACT 
CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT CCAGAAGATT 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCGGT CTGAGGCTTC AGATGCCACG 
GAGGCATCTT TTAGTCTGAG AATTCGGCCG TGCGTTGAGG ACAAACTAGG GAATTCAGCT 
TCACAAGCAC AGGTTTCAGA CACTGACGTT ACTACAAGTC CAAAAGGGAA AGGTGACAGA 
CCTCCTCAGC ATGACAGGGA GCTGAGGCCT AATAGGAAAT GTAGCCGAAA ACGTGGATTT 
CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATGGAAG AGATAGAAAA TTTGAAGAAA 
CAACATGATT TATTAAAAAG GATGTTACAG CAGCAGGAGC AGCTGAGAGC TCTACAAGGA 
CGACAAGCTG CTCTGCTTGC CCTGCAGCAT AAAGCTGAGC AGGCTATAGC TGTGATGGAT 
GATTCTGAAA AGGTTGCGGA GACAACTCCT GGCCATCATA CTGTACCAGG CAGACAGCCG 
GCTCATTCAC CTTTGCATCA GAGAGTACCC ATAAGAGAAA CTACAGGTAG CCTATCTGGA 
GTCAGTATCA CCTCTGAACT AAACGAAGAA TTGAATGATT TAATTCAGCA TTTTCATAAT 
CAGCTTCGTG ATTCTCAGCC TCCAGCTGTT CCAGACAATA GAAGACAAGC AGAAAGTCTT 
TCATTAACTA GGGAGATTTC TCAGAGCAGA AATCCTTCAG TTTCAGAGCA TTTACCTGAT 
GAGAAAGTCC AACTTTTTAG CAAGATGAGA GTGTTACAAG AAAAGAAACA AAAAATGGAC 
AAACTTCTTG GAGAACTTCA TACACTTCGA GATCAGCATT TGAACAATTC ATCATTTGTG 
CCTTCTTCAG CCTCTCCACA AAGGAGTGTG GATCAGAGAA GTACAACCAC AGCTCCTTCT 
GCTCCTGTAG TCTTAACACC AGTGGTCAAT GGAGAATCCA ATAACCTCAC GCCATCTGTT 
CCTTATCCTG CTGCTTCTCT AGTTTCTCAG AATCAGAATG AAAATGAAGG CCACCTAAAT 
CCAACTGAAA AACTCCAGAA GTTAAATGAA GTTCGAAAGA GGTTGAATGA ACTAAGAGAA 
TTAGTTCATT ATTATGAGCA AACATCAGAT ATGATGACAG ATGCTGTGAA TGAAAACACA 
AAAGAAGAAG AAACTGAAGA ATCAGAATAT GATTCGGAGC ATGAAAACTC GGAACCTGTT 
ACTAACATTC GAAATCCACA AGTGGCTGCC ACCTGGAACG AAGTAAATAG TAATTCTAAT 
GCACAGTGTG TTTCCAACAA TAGAGATGGG CGAGCAGTTA ATTCTAACTG TGAAATTAAC 
AACAGGTCTG CTGCCAACAT AAGGGCTCTA AACATGCCTC CTCTTTCAGA TTGTCGATAC 
AATAGAGAAG GAGAACAGGA GATGCCTGTT GCACAAGGTC ATGAGGAGGA GGAGGAGGAG 
GAGGAGGAAG CAGAAGATGA GGCCCTCAGT GGAGCTTCAC TGTCTAGTCA CAGCAGCAGC 
CTGATCATTG AGACTCCTGA AGATGCTGAA TTTGAACATA AGATCCAGAA GCTTATGGCT 
GCAAAACTGA AACTTAGACA CTTACAGAAC CTTGTAGCCA TGGTGCAGGA CGATGATGCA 
GCCAATCAAG GAGTTACTTC TGTGGAGGAT TTCTTCCCAG CAGAAGACAC CAAACAAAAT 
GCAAATAACA CTAGAGGAAA TGCCAATAAA ACACAGAAAG ATGCTGGAGT AAATGAAAAG 
GCAAGGGAGA AATTTTATGA GGCTAAACTA CAGCAGCAAC AGAGAGAACT CAAACAATTG 
CAGGAAGAAA GAAAGAAACT GATTGAGATT CAAGAGAAAA TTCAAGAATT GCAAAAGGCA 
TGTCCTAACC TACAGCTGTC AGCTGCTAGT GTGGGTAATT GTCCCACAAA AACACATATG 
CCAGCTGTTA CTTCATCCCC AACCGTTAAT GAAAATGAAG CCAGTACAAA CAAATCTGGT 
TTTGAGCCTC ATGATTCTTC AGTGGTAGAT AATGAGGTAT GGTCAGATAT GCGAAGACAT 
GAAATGTTAA GGGAGGAGCT ACGACAGAGA AGAAAGCAGC TGGAAGCTCT GATGGCTGAA 
CATCAGAGGA GGCAAGGTCT GGCTGAAACT GCATCTCCAG TGGCTGTTTC ACTGAGAAGT 
GATGGATCTG AGAACCTGTG TACACCTCAG CAAAGCAGAA CAGAAAAAAC AATGGCAACT 
TGGGGAGGTT CTACCCAGTG TGCCCTAGAT GAAGAAGGAG ATGAAGATGG TTACCTTTCT 
GAAGGAATTA TTCGGACAGA CGAAGAGGAG GAAGAGGAGG AGCAAGATGC CAGTTCCAAT 
GATAACTTTC ATATGTATCC TCCAAACAGT GCAAATCATA ATTCTTACAA TGTAAAGGAA 
ACTAAAAATA GGTGGAAGAA CAATCGCCCT TTTTCAGCAG ATGGAAATTA TCGTCCTTTA 
GCCAAGACAA GACAGCAGAA TATCAGCATG CAGCGTCAGG AAAACCTTCG CTGGGTGTCC 
GAACTCTCTT ATGTAGAAGA GAAAGAACAA TGGCAAGAAC AAATTAATCA GCTAAAGAAA 
CAACTTGATT TCAGTGTCAA TATTTGTCAG ACTTTAATGC AAGACCAGCA GACTCTGTCT 
TGTCTGCTAC AAACTCTTCT CACGAGTCCT TATAGTGTTA TGCCCAGCAG TGTTGCATCC 
CCGCAAGTGC ACCTTATAAT GCACCAGTTG AATCAGTGCT ATACCCAGCT AACGTGGCAG 
CAGAACAATG TCCAGAGGTT GAAACAAATG CTCAGTGAAC TCATGCGCCA ACAAAATCAG 
CATCCAGAAA AACCTGCAAG CAAGGAAGGA GGCAGTGGTA CATCACACCC TCCTTCTCCC 
AGTTTATTTT GTCCTTTCAA CTTTCCAACT CAGCCTGTAA ATCTGTTTAA CCTACCGGGG 
TTTACTAATT TTTCGTCATT TGCACCAAGT ATGAATTTCA GCCCTTTATT TCCTTCTAAT 
TTTGGAGATT TTTCTCAAAA TATTTCTACA CCCACTGAAC AGCAGCAACC GTTAGCCCAG 
AATCCTTCAG GGAAAACGGA ATACATGGCT TTTCCAAAAC CTTTTGAAAG TAGTTCTTCT 
ATTGGAGCGG AAAAACAAAG GAATCAAAAA CAGCCTGAAG AGGAGGTGGA GAACAGTAGG 
ACACCATGGT TTTATGACCA AGAAGGTGAA GTAGAAAAGC CATTTCTCAA GACTGGATTT 
GCAGTGTCTG TAGAGAAAAC AACAAATAGT AATCGCAAAA ATCAACTAGA TACCAGTAGG 
AGAAGACGCC AGTTTGATGA AGTGTCCTTA GAAAGCTTTA ACAGTATGCT TGACCCAGTA 
GATCCAACAA CAGTAACTAA AACATTCAAG TCAAGAAAAG CATCTGCACA GGCCAGCCTG 
GCATCTAAAG ATAAAACTCC CAAATCAAAG AGTAAGAAGA GGCATTCTAC TCAGCTGAAA 
AGCAGAGTGA AAAATATCGG GTATGAAAGT GCCAGTGTGT CTAGCACATG TGAACCTTGC 
AAAAGTAGAA ACAGACATTC AGCCCAAACT GAAGAGCCTG TTCAAGCAAA AGTACTCAGC 
AGAAAGAATC ATGAGCAGCT GGAAAAAGTC ATAAGATGTA GTAGGTCTAC AGAAATATCT 
TCAGAAACCG GGAGTGATTT TTCCATGTTC GAAGCTTTGC GGGATACCAT TTATTCTGAA 
GTAGCTACGT TGATTTCTCA AAATGAATCT CGTCCACATT TTCTTATTGA ACTCTTCCAT 
GAGCTTCAGC TACTTAATAC AGACTATTTG AGACAGAGGG CCTTATATGC ATTGCAGGAC 
ATAGTATCCA GGCATATTTC TGAGAACCAT GAAAAAGAAG GAGAAAATAT AAAGTCCGTG 
AATTCTGGTA CTTGGATAGC ATCCAACTCA GAACTTACTC CCAGTGAAAG CCTTGCTACT 
ACTGATGATG AAACTTTTGA GAAGAACTTT GAGAGAGAAA CACATAAAAT AAGTGAGCAA 
AATGATGCTG ATAATGCTAG TGTATCTTCA ACTTGTGAGC CTTTTGCAAC AGATGTTCTG 
GGCGACACTG TGATTCACTT AGATCAGGCA TTAGCCAGAA TGAGAGACTA TACGCATATG 
AAGATGGAGG CTGAAAGTAG TCCAGACGTC AGGTGCACCT GCAGGATCAT TGAGGATGAC 
GATGGTGCTG CTGCCACAAC CACGATTAGT AATAATTTAG AAGAAACTCC TGTTATTGAA 
AATCATAGTT CACAGCAACC CGTGAGTGAA GTTGCTACCG TCCCGTGTCC TAGAATTGAT 
ACTCAGCAAC TGGACCGGCA AATTAAAGCA ATTATGAAAG AAGTCATTCC TTTTTTGAAG 
GAGCACATGG ATGAAGTATG TTCTTCTCAA CTTCTAACCT CAGTAAGACG CATGGTTTTG 
GTCCTTACTC AGCAAAACAA TGAGAGCAAA GAGTTTGTAA AGTTCTTTCA TAAGCAGCTT 
GGAAGTATAT TACAGGATTC ACTCGCAAAA TTTGCTGGCA GAAAACTGAA GGATTGTGGA 
GAAGATCTTC TTGTAGAGAT ATCTGAAGTG TTATTTAATG AATTGGCTTT CTTTAAGCTC 
ATGCAAGATT TGGATAATAA TAGTGTAACT GTTAAACAGA GATGCAAAAG GAAAATAGAA 
GCTGCTGGAG TGATACAGTC TTATGCAAAA GAGGCCAAAA GGATTCTTGA AGGAGATCAC 
AGTTCACCTG CTGGAGAAAT TGATGATGAA GACAAAGACA AGGATGAGAC GGAAACAGTT 
AAGCAGACTC AAACGTCTGA GGTTTATGAT GGTGACGGCC CCAAAAATGT GAGATCTGAT 
GTTTCTGATC AAGAGGATGA TGAAGAAAGT GAAGAATGTC CAGTGTCTAT TAATTTGTCC 
AAAGCTGAAA CTCAGGCTTT GACTAATTAC GGAAGTGGAG AAGATGAGAA TGAAGATGAA 
GAAATAGAAG AATTTGAAGA GGGACCTGTA GATGTCCAGA CGTCACTTCA GGCTAACACT 
GAAACTACAG AAGAAAATGA ACACGATGAT CAGGTTCCAC AGCATGATTT TGAAAAATCA 
TCAGAAAGCA AAAATGTCCC ATCAGAACAA GAACCCACTA CTAGTAAAGA CTTTCTTTTG 
ATGACAGGTG ACCAGAATAG CACTCCTGTG AAGCCCTGTT ACCTCAGTAT CGTGGAAAAT 
GAGCAACCTT TAAATAGCCC TGTCCAGAAG GACACTCTCA CTACCATCGA CTCATCCAGT 
CAGCCTAACC CTTTGCCTTT ACCTTTAACT GAAATTGAAA CCTTGGTGCC TAGAGTCAAA 
GAAGTGAAAT CTGCTCAGGA GACTCCTGAA AGTTCTTTGG CTGGAAGTCC TGATACTGAA 
TCTCCAGTGT TAGTGAATGA CTATGAAGCA GAATCTGGTA ACATAAGTCA AAAATCTGAT 
GAAGAAGACT TTGTGAAAGT TGAAGATTTA CCACTTAAAC TGACAATATA TTCAGAGGCA 
GATCTAAGGA AGAAAATGGC CGAAGAAGAA CAGAAAAACC ACTTATCTGG TGAAATATGT 
GCAATGCAGA CTGAAGAATT AGCTGGGAAT TCTCAGACAC TAAAAGAACC TGAGCAAGTA 
TGTGCTCAGT CTGCCATCTT AAGATGGAAA CCCTTACCTG TGCCTTTTAA ACTGTTGGTG 
GTGTCATCAG GGAATTATTG TAGCAAAAAA CAGTGGGAGC CCAAAGTACA TGAGATGTCT 
CCAGAGGCTC AGCTAACTCT TTCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( XM_025279446.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025135231.1 Bubalus bubalis pericentriolar material 1 (PCM1), transcript variant X7, mRNA.

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CloneID	OBu42295
Clone ID Related Accession (Same CDS sequence)	XM_025279446.1
Accession Version	XM_025279446.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6624bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	pericentriolar material 1 protein isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037545.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG GAGGAGGTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
AGCAATGAGA GTGTTGATGA CCGACTCAAC AATATGGATT GGGGCAGCCA GCAGAAGAAG 
GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA 
ACTAACGATA TTTCTCCGGA GTCATCACCG GGAGTTGGAA GACGAAGAAC AAAGACTTCA 
CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TCCCCGAGCA GGCAGAACTA 
GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCTCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCCAC TGTAAGTCCT 
CAAAAGAGAG AAATGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAGATTCGTG AGTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGACCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTCACT 
CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT CCAGAAGATT 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCGGT CTGAGGCTTC AGATGCCACG 
GAGGCATCTT TTAGTCTGAG AATTCGGCCG TGCGTTGAGG ACAAACTAGG GAATTCAGCT 
TCACAAGCAC AGGTTTCAGA CACTGACGTT ACTACAAGTC CAAAAGGGAA AGGTGACAGA 
CCTCCTCAGC ATGACAGGGA GCTGAGGCCT AATAGGAAAT GTAGCCGAAA ACGTGGATTT 
CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATGGAAG AGATAGAAAA TTTGAAGAAA 
CAACATGATT TATTAAAAAG GATGTTACAG CAGCAGGAGC AGCTGAGAGC TCTACAAGGA 
CGACAAGCTG CTCTGCTTGC CCTGCAGCAT AAAGCTGAGC AGGCTATAGC TGTGATGGAT 
GATTCTGAAA AGGTTGCGGA GACAACTCCT GGCCATCATA CTGTACCAGG CAGACAGCCG 
GCTCATTCAC CTTTGCATCA GAGAGTACCC ATAAGAGAAA CTACAGGTAG CCTATCTGGA 
GTCAGTATCA CCTCTGAACT AAACGAAGAA TTGAATGATT TAATTCAGCA TTTTCATAAT 
CAGCTTCGTG ATTCTCAGCC TCCAGCTGTT CCAGACAATA GAAGACAAGC AGAAAGTCTT 
TCATTAACTA GGGAGATTTC TCAGAGCAGA AATCCTTCAG TTTCAGAGCA TTTACCTGAT 
GAGAAAGTCC AACTTTTTAG CAAGATGAGA GTGTTACAAG AAAAGAAACA AAAAATGGAC 
AAACTTCTTG GAGAACTTCA TACACTTCGA GATCAGCATT TGAACAATTC ATCATTTGTG 
CCTTCTTCAG CCTCTCCACA AAGGAGTGTG GATCAGAGAA GTACAACCAC AGCTCCTTCT 
GCTCCTGTAG TCTTAACACC AGTGGTCAAT GGAGAATCCA ATAACCTCAC GCCATCTGTT 
CCTTATCCTG CTGCTTCTCT AGTTTCTCAG AATCAGAATG AAAATGAAGG CCACCTAAAT 
CCAACTGAAA AACTCCAGAA GTTAAATGAA GTTCGAAAGA GGTTGAATGA ACTAAGAGAA 
TTAGTTCATT ATTATGAGCA AACATCAGAT ATGATGACAG ATGCTGTGAA TGAAAACACA 
AAAGAAGAAG AAACTGAAGA ATCAGAATAT GATTCGGAGC ATGAAAACTC GGAACCTGTT 
ACTAACATTC GAAATCCACA AGTGGCTGCC ACCTGGAACG AAGTAAATAG TAATTCTAAT 
GCACAGTGTG TTTCCAACAA TAGAGATGGG CGAGCAGTTA ATTCTAACTG TGAAATTAAC 
AACAGGTCTG CTGCCAACAT AAGGGCTCTA AACATGCCTC CTCTTTCAGC AGATTGTCGA 
TACAATAGAG AAGGAGAACA GGAGATGCCT GTTGCACAAG GTCATGAGGA GGAGGAGGAG 
GAGGAGGAGG AAGCAGAAGA TGAGGCCCTC AGTGGAGCTT CACTGTCTAG TCACAGCAGC 
AGCCTGATCA TTGAGACTCC TGAAGATGCT GAATTTGAAC ATAAGATCCA GAAGCTTATG 
GCTGCAAAAC TGAAACTTAG ACACTTACAG AACCTTGTAG CCATGGTGCA GGACGATGAT 
GCAGCCAATC AAGGAGTTAC TTCTGTGGAG GATTTCTTCC CAGCAGAAGA CACCAAACAA 
AATGCAAATA ACACTAGAGG AAATGCCAAT AAAACACAGA AAGATGCTGG AGTAAATGAA 
AAGGCAAGGG AGAAATTTTA TGAGGCTAAA CTACAGCAGC AACAGAGAGA ACTCAAACAA 
TTGCAGGAAG AAAGAAAGAA ACTGATTGAG ATTCAAGAGA AAATTCAAGA ATTGCAAAAG 
GCATGTCCTA ACCTACAGCT GTCAGCTGCT AGTGTGGGTA ATTGTCCCAC AAAAACACAT 
ATGCCAGCTG TTACTTCATC CCCAACCGTT AATGAAAATG AAGCCAGTAC AAACAAATCT 
GGTTTTGAGC CTCATGATTC TTCAGTGGTA GATAATGAGG TATGGTCAGA TATGCGAAGA 
CATGAAATGT TAAGGGAGGA GCTACGACAG AGAAGAAAGC AGCTGGAAGC TCTGATGGCT 
GAACATCAGA GGAGGCAAGG TCTGGCTGAA ACTGCATCTC CAGTGGCTGT TTCACTGAGA 
AGTGATGGAT CTGAGAACCT GTGTACACCT CAGCAAAGCA GAACAGAAAA AACAATGGCA 
ACTTGGGGAG GTTCTACCCA GTGTGCCCTA GATGAAGAAG GAGATGAAGA TGGTTACCTT 
TCTGAAGGAA TTATTCGGAC AGACGAAGAG GAGGAAGAGG AGGAGCAAGA TGCCAGTTCC 
AATGATAACT TTCATATGTA TCCTCCAAAC AGTGCAAATC ATAATTCTTA CAATGTAAAG 
GAAACTAAAA ATAGGTGGAA GAACAATCGC CCTTTTTCAG CAGATGGAAA TTATCGTCCT 
TTAGCCAAGA CAAGACAGCA GAATATCAGC ATGCAGCGTC AGGAAAACCT TCGCTGGGTG 
TCCGAACTCT CTTATGTAGA AGAGAAAGAA CAATGGCAAG AACAAATTAA TCAGCTAAAG 
AAACAACTTG ATTTCAGTGT CAATATTTGT CAGACTTTAA TGCAAGACCA GCAGACTCTG 
TCTTGTCTGC TACAAACTCT TCTCACGAGT CCTTATAGTG TTATGCCCAG CAGTGTTGCA 
TCCCCGCAAG TGCACCTTAT AATGCACCAG TTGAATCAGT GCTATACCCA GCTAACGTGG 
CAGCAGAACA ATGTCCAGAG GTTGAAACAA ATGCTCAGTG AACTCATGCG CCAACAAAAT 
CAGCATCCAG AAAAACCTGC AAGCAAGGAA GGAGGCAGTG GTACATCACA CCCTCCTTCT 
CCCAGTTTAT TTTGTCCTTT CAACTTTCCA ACTCAGCCTG TAAATCTGTT TAACCTACCG 
GGGTTTACTA ATTTTTCGTC ATTTGCACCA AGTATGAATT TCAGCCCTTT ATTTCCTTCT 
AATTTTGGAG ATTTTTCTCA AAATATTTCT ACACCCACTG AACAGCAGCA ACCGTTAGCC 
CAGAATCCTT CAGGGAAAAC GGAATACATG GCTTTTCCAA AACCTTTTGA AAGTAGTTCT 
TCTATTGGAG CGGAAAAACA AAGGAATCAA AAACAGCCTG AAGAGGAGGT GGAGAACAGT 
AGGACACCAT GGTTTTATGA CCAAGAAGGT GAAGTAGAAA AGCCATTTCT CAAGACTGGA 
TTTGCAGTGT CTGTAGAGAA AACAACAAAT AGTAATCGCA AAAATCAACT AGATACCAGT 
AGGAGAAGAC GCCAGTTTGA TGAAGTGTCC TTAGAAAGCT TTAACAGTAT GCTTGACCCA 
GTAGATCCAA CAACAGTAAC TAAAACATTC AAGTCAAGAA AAGCATCTGC ACAGGCCAGC 
CTGGCATCTA AAGATAAAAC TCCCAAATCA AAGAGTAAGA AGAGGCATTC TACTCAGCTG 
AAAAGCAGAG TGAAAAATAT CGGGTATGAA AGTGCCAGTG TGTCTAGCAC ATGTGAACCT 
TGCAAAAGTA GAAACAGACA TTCAGCCCAA ACTGAAGAGC CTGTTCAAGC AAAAGTACTC 
AGCAGAAAGA ATCATGAGCA GCTGGAAAAA GTCATAAGAT GTAGTAGGTC TACAGAAATA 
TCTTCAGCAC ATGCTAGGAG AATACTACAG CAGTCTAACA GAAATGCATG CAATGAAGCG 
CCAGAAACCG GGAGTGATTT TTCCATGTTC GAAGCTTTGC GGGATACCAT TTATTCTGAA 
GTAGCTACGT TGATTTCTCA AAATGAATCT CGTCCACATT TTCTTATTGA ACTCTTCCAT 
GAGCTTCAGC TACTTAATAC AGACTATTTG AGACAGAGGG CCTTATATGC ATTGCAGGAC 
ATAGTATCCA GGCATATTTC TGAGAACCAT GAAAAAGAAG GAGAAAATAT AAAGTCCGTG 
AATTCTGGTA CTTGGATAGC ATCCAACTCA GAACTTACTC CCAGTGAAAG CCTTGCTACT 
ACTGATGATG AAACTTTTGA GAAGAACTTT GAGAGAGAAA CACATAAAAT AAGTGAGCAA 
AATGATGCTG ATAATGCTAG TGTATCTTCA ACTTGTGAGC CTTTTGCAAC AGATGTTCTG 
GGCGACACTG TGATTCACTT AGATCAGGCA TTAGCCAGAA TGAGAGACTA TACGCATATG 
AAGATGGAGG CTGAAAGTAG TCCAGACGTC AGGTGCACCT GCAGGATCAT TGAGGATGAC 
GATGGTGCTG CTGCCACAAC CACGATTAGT AATAATTTAG AAGAAACTCC TGTTATTGAA 
AATCATAGTT CACAGCAACC CGTGAGTGAA GTTGCTACCG TCCCGTGTCC TAGAATTGAT 
ACTCAGCAAC TGGACCGGCA AATTAAAGCA ATTATGAAAG AAGTCATTCC TTTTTTGAAG 
GAGCACATGG ATGAAGTATG TTCTTCTCAA CTTCTAACCT CAGTAAGACG CATGGTTTTG 
GTCCTTACTC AGCAAAACAA TGAGAGCAAA GAGTTTGTAA AGTTCTTTCA TAAGCAGCTT 
GGAAGTATAT TACAGGATTC ACTCGCAAAA TTTGCTGGCA GAAAACTGAA GGATTGTGGA 
GAAGATCTTC TTGTAGAGAT ATCTGAAGTG TTATTTAATG AATTGGCTTT CTTTAAGCTC 
ATGCAAGATT TGGATAATAA TAGTGTAACT GTTAAACAGA GATGCAAAAG GAAAATAGAA 
GCTGCTGGAG TGATACAGTC TTATGCAAAA GAGGACAAGG ATGAGACGGA AACAGTTAAG 
CAGACTCAAA CGTCTGAGGT TTATGATGGT GACGGCCCCA AAAATGTGAG ATCTGATGTT 
TCTGATCAAG AGGATGATGA AGAAAGTGAA GAATGTCCAG TGTCTATTAA TTTGTCCAAA 
GCTGAAACTC AGGCTTTGAC TAATTACGGA AGTGGAGAAG ATGAGAATGA AGATGAAGAA 
ATAGAAGAAT TTGAAGAGGG ACCTGTAGAT GTCCAGACGT CACTTCAGGC TAACACTGAA 
ACTACAGAAG AAAATGAACA CGATGATCAG GTTCCACAGC ATGATTTTGA AAAATCATCA 
GAAAGCAAAA ATGTCCCATC AGAACAAGAA CCCACTACTA GTAAAGACTT TCTTTTGATG 
ACAGGTGACC AGAATAGCAC TCCTGTGAAG CCCTGTTACC TCAGTATCGT GGAAAATGAG 
CAACCTTTAA ATAGCCCTGT CCAGAAGGAC ACTCTCACTA CCATCGACTC ATCCAGTCAG 
CCTAACCCTT TGCCTTTACC TTTAACTGAA ATTGAAACCT TGGTGCCTAG AGTCAAAGAA 
GTGAAATCTG CTCAGGAGAC TCCTGAAAGT TCTTTGGCTG GAAGTCCTGA TACTGAATCT 
CCAGTGTTAG TGAATGACTA TGAAGCAGAA TCTGGTAACA TAAGTCAAAA ATCTGATGAA 
GAAGACTTTG TGAAAGTTGA AGATTTACCA CTTAAACTGA CAATATATTC AGAGGCAGAT 
CTAAGGAAGA AAATGGCCGA AGAAGAACAG AAAAACCACT TATCTGGTGA AATATGTGCA 
ATGCAGACTG AAGAATTAGC TGGGAATTCT CAGACACTAA AAGAACCTGA GCAAGTATGT 
GCTCAGTCTG CCATCTTAAG ATGGAAACCC TTACCTGTGC CTTTTAAACT GTTGGTGGTG 
TCATCAGGGA ATTATTGTAG CAAAAAACAG TGGGAGCCCA AAGTACATGA GATGTCTCCA 
GAGGCTCAGC TAACTCTTTC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025135231.1
CDS	392..7015
Translation	MATGGGPFEEGVNDQDLPNWSNESVDDRLNNMDWGSQQKKANRSSEKNKRKFGVESDKRVTNDISPESSPGVGRRRTKTSLTFPHSRYMTQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDATVSPQKREMIGSAQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIREYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLQKILARENEEEDVRTIDSAVGSGSVAESTSLNIDVRSEASDATEASFSLRIRPCVEDKLGNSASQAQVSDTDVTTSPKGKGDRPPQHDRELRPNRKCSRKRGFPSKARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSEKVAETTPGHHTVPGRQPAHSPLHQRVPIRETTGSLSGVSITSELNEELNDLIQHFHNQLRDSQPPAVPDNRRQAESLSLTREISQSRNPSVSEHLPDEKVQLFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSFVPSSASPQRSVDQRSTTTAPSAPVVLTPVVNGESNNLTPSVPYPAASLVSQNQNENEGHLNPTEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENTKEEETEESEYDSEHENSEPVTNIRNPQVAATWNEVNSNSNAQCVSNNRDGRAVNSNCEINNRSAANIRALNMPPLSADCRYNREGEQEMPVAQGHEEEEEEEEEAEDEALSGASLSSHSSSLIIETPEDAEFEHKIQKLMAAKLKLRHLQNLVAMVQDDDAANQGVTSVEDFFPAEDTKQNANNTRGNANKTQKDAGVNEKAREKFYEAKLQQQQRELKQLQEERKKLIEIQEKIQELQKACPNLQLSAASVGNCPTKTHMPAVTSSPTVNENEASTNKSGFEPHDSSVVDNEVWSDMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETASPVAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGIIRTDEEEEEEEQDASSNDNFHMYPPNSANHNSYNVKETKNRWKNNRPFSADGNYRPLAKTRQQNISMQRQENLRWVSELSYVEEKEQWQEQINQLKKQLDFSVNICQTLMQDQQTLSCLLQTLLTSPYSVMPSSVASPQVHLIMHQLNQCYTQLTWQQNNVQRLKQMLSELMRQQNQHPEKPASKEGGSGTSHPPSPSLFCPFNFPTQPVNLFNLPGFTNFSSFAPSMNFSPLFPSNFGDFSQNISTPTEQQQPLAQNPSGKTEYMAFPKPFESSSSIGAEKQRNQKQPEEEVENSRTPWFYDQEGEVEKPFLKTGFAVSVEKTTNSNRKNQLDTSRRRRQFDEVSLESFNSMLDPVDPTTVTKTFKSRKASAQASLASKDKTPKSKSKKRHSTQLKSRVKNIGYESASVSSTCEPCKSRNRHSAQTEEPVQAKVLSRKNHEQLEKVIRCSRSTEISSAHARRILQQSNRNACNEAPETGSDFSMFEALRDTIYSEVATLISQNESRPHFLIELFHELQLLNTDYLRQRALYALQDIVSRHISENHEKEGENIKSVNSGTWIASNSELTPSESLATTDDETFEKNFERETHKISEQNDADNASVSSTCEPFATDVLGDTVIHLDQALARMRDYTHMKMEAESSPDVRCTCRIIEDDDGAAATTTISNNLEETPVIENHSSQQPVSEVATVPCPRIDTQQLDRQIKAIMKEVIPFLKEHMDEVCSSQLLTSVRRMVLVLTQQNNESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSVTVKQRCKRKIEAAGVIQSYAKEDKDETETVKQTQTSEVYDGDGPKNVRSDVSDQEDDEESEECPVSINLSKAETQALTNYGSGEDENEDEEIEEFEEGPVDVQTSLQANTETTEENEHDDQVPQHDFEKSSESKNVPSEQEPTTSKDFLLMTGDQNSTPVKPCYLSIVENEQPLNSPVQKDTLTTIDSSSQPNPLPLPLTEIETLVPRVKEVKSAQETPESSLAGSPDTESPVLVNDYEAESGNISQKSDEEDFVKVEDLPLKLTIYSEADLRKKMAEEEQKNHLSGEICAMQTEELAGNSQTLKEPEQVCAQSAILRWKPLPVPFKLLVVSSGNYCSKKQWEPKVHEMSPEAQLTLS

Target ORF information:

RefSeq Version	XM_025279446.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis pericentriolar material 1 (PCM1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025279446.1

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
AGCAATGAGA GTGTTGATGA CCGACTCAAC AATATGGATT GGGGCAGCCA GCAGAAGAAG 
GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA 
ACTAACGATA TTTCTCCGGA GTCATCACCG GGAGTTGGAA GACGAAGAAC AAAGACTTCA 
CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TCCCCGAGCA GGCAGAACTA 
GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCTCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCCAC TGTAAGTCCT 
CAAAAGAGAG AAATGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAGATTCGTG AGTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGACCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTCACT 
CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT CCAGAAGATT 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCGGT CTGAGGCTTC AGATGCCACG 
GAGGCATCTT TTAGTCTGAG AATTCGGCCG TGCGTTGAGG ACAAACTAGG GAATTCAGCT 
TCACAAGCAC AGGTTTCAGA CACTGACGTT ACTACAAGTC CAAAAGGGAA AGGTGACAGA 
CCTCCTCAGC ATGACAGGGA GCTGAGGCCT AATAGGAAAT GTAGCCGAAA ACGTGGATTT 
CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATGGAAG AGATAGAAAA TTTGAAGAAA 
CAACATGATT TATTAAAAAG GATGTTACAG CAGCAGGAGC AGCTGAGAGC TCTACAAGGA 
CGACAAGCTG CTCTGCTTGC CCTGCAGCAT AAAGCTGAGC AGGCTATAGC TGTGATGGAT 
GATTCTGAAA AGGTTGCGGA GACAACTCCT GGCCATCATA CTGTACCAGG CAGACAGCCG 
GCTCATTCAC CTTTGCATCA GAGAGTACCC ATAAGAGAAA CTACAGGTAG CCTATCTGGA 
GTCAGTATCA CCTCTGAACT AAACGAAGAA TTGAATGATT TAATTCAGCA TTTTCATAAT 
CAGCTTCGTG ATTCTCAGCC TCCAGCTGTT CCAGACAATA GAAGACAAGC AGAAAGTCTT 
TCATTAACTA GGGAGATTTC TCAGAGCAGA AATCCTTCAG TTTCAGAGCA TTTACCTGAT 
GAGAAAGTCC AACTTTTTAG CAAGATGAGA GTGTTACAAG AAAAGAAACA AAAAATGGAC 
AAACTTCTTG GAGAACTTCA TACACTTCGA GATCAGCATT TGAACAATTC ATCATTTGTG 
CCTTCTTCAG CCTCTCCACA AAGGAGTGTG GATCAGAGAA GTACAACCAC AGCTCCTTCT 
GCTCCTGTAG TCTTAACACC AGTGGTCAAT GGAGAATCCA ATAACCTCAC GCCATCTGTT 
CCTTATCCTG CTGCTTCTCT AGTTTCTCAG AATCAGAATG AAAATGAAGG CCACCTAAAT 
CCAACTGAAA AACTCCAGAA GTTAAATGAA GTTCGAAAGA GGTTGAATGA ACTAAGAGAA 
TTAGTTCATT ATTATGAGCA AACATCAGAT ATGATGACAG ATGCTGTGAA TGAAAACACA 
AAAGAAGAAG AAACTGAAGA ATCAGAATAT GATTCGGAGC ATGAAAACTC GGAACCTGTT 
ACTAACATTC GAAATCCACA AGTGGCTGCC ACCTGGAACG AAGTAAATAG TAATTCTAAT 
GCACAGTGTG TTTCCAACAA TAGAGATGGG CGAGCAGTTA ATTCTAACTG TGAAATTAAC 
AACAGGTCTG CTGCCAACAT AAGGGCTCTA AACATGCCTC CTCTTTCAGC AGATTGTCGA 
TACAATAGAG AAGGAGAACA GGAGATGCCT GTTGCACAAG GTCATGAGGA GGAGGAGGAG 
GAGGAGGAGG AAGCAGAAGA TGAGGCCCTC AGTGGAGCTT CACTGTCTAG TCACAGCAGC 
AGCCTGATCA TTGAGACTCC TGAAGATGCT GAATTTGAAC ATAAGATCCA GAAGCTTATG 
GCTGCAAAAC TGAAACTTAG ACACTTACAG AACCTTGTAG CCATGGTGCA GGACGATGAT 
GCAGCCAATC AAGGAGTTAC TTCTGTGGAG GATTTCTTCC CAGCAGAAGA CACCAAACAA 
AATGCAAATA ACACTAGAGG AAATGCCAAT AAAACACAGA AAGATGCTGG AGTAAATGAA 
AAGGCAAGGG AGAAATTTTA TGAGGCTAAA CTACAGCAGC AACAGAGAGA ACTCAAACAA 
TTGCAGGAAG AAAGAAAGAA ACTGATTGAG ATTCAAGAGA AAATTCAAGA ATTGCAAAAG 
GCATGTCCTA ACCTACAGCT GTCAGCTGCT AGTGTGGGTA ATTGTCCCAC AAAAACACAT 
ATGCCAGCTG TTACTTCATC CCCAACCGTT AATGAAAATG AAGCCAGTAC AAACAAATCT 
GGTTTTGAGC CTCATGATTC TTCAGTGGTA GATAATGAGG TATGGTCAGA TATGCGAAGA 
CATGAAATGT TAAGGGAGGA GCTACGACAG AGAAGAAAGC AGCTGGAAGC TCTGATGGCT 
GAACATCAGA GGAGGCAAGG TCTGGCTGAA ACTGCATCTC CAGTGGCTGT TTCACTGAGA 
AGTGATGGAT CTGAGAACCT GTGTACACCT CAGCAAAGCA GAACAGAAAA AACAATGGCA 
ACTTGGGGAG GTTCTACCCA GTGTGCCCTA GATGAAGAAG GAGATGAAGA TGGTTACCTT 
TCTGAAGGAA TTATTCGGAC AGACGAAGAG GAGGAAGAGG AGGAGCAAGA TGCCAGTTCC 
AATGATAACT TTCATATGTA TCCTCCAAAC AGTGCAAATC ATAATTCTTA CAATGTAAAG 
GAAACTAAAA ATAGGTGGAA GAACAATCGC CCTTTTTCAG CAGATGGAAA TTATCGTCCT 
TTAGCCAAGA CAAGACAGCA GAATATCAGC ATGCAGCGTC AGGAAAACCT TCGCTGGGTG 
TCCGAACTCT CTTATGTAGA AGAGAAAGAA CAATGGCAAG AACAAATTAA TCAGCTAAAG 
AAACAACTTG ATTTCAGTGT CAATATTTGT CAGACTTTAA TGCAAGACCA GCAGACTCTG 
TCTTGTCTGC TACAAACTCT TCTCACGAGT CCTTATAGTG TTATGCCCAG CAGTGTTGCA 
TCCCCGCAAG TGCACCTTAT AATGCACCAG TTGAATCAGT GCTATACCCA GCTAACGTGG 
CAGCAGAACA ATGTCCAGAG GTTGAAACAA ATGCTCAGTG AACTCATGCG CCAACAAAAT 
CAGCATCCAG AAAAACCTGC AAGCAAGGAA GGAGGCAGTG GTACATCACA CCCTCCTTCT 
CCCAGTTTAT TTTGTCCTTT CAACTTTCCA ACTCAGCCTG TAAATCTGTT TAACCTACCG 
GGGTTTACTA ATTTTTCGTC ATTTGCACCA AGTATGAATT TCAGCCCTTT ATTTCCTTCT 
AATTTTGGAG ATTTTTCTCA AAATATTTCT ACACCCACTG AACAGCAGCA ACCGTTAGCC 
CAGAATCCTT CAGGGAAAAC GGAATACATG GCTTTTCCAA AACCTTTTGA AAGTAGTTCT 
TCTATTGGAG CGGAAAAACA AAGGAATCAA AAACAGCCTG AAGAGGAGGT GGAGAACAGT 
AGGACACCAT GGTTTTATGA CCAAGAAGGT GAAGTAGAAA AGCCATTTCT CAAGACTGGA 
TTTGCAGTGT CTGTAGAGAA AACAACAAAT AGTAATCGCA AAAATCAACT AGATACCAGT 
AGGAGAAGAC GCCAGTTTGA TGAAGTGTCC TTAGAAAGCT TTAACAGTAT GCTTGACCCA 
GTAGATCCAA CAACAGTAAC TAAAACATTC AAGTCAAGAA AAGCATCTGC ACAGGCCAGC 
CTGGCATCTA AAGATAAAAC TCCCAAATCA AAGAGTAAGA AGAGGCATTC TACTCAGCTG 
AAAAGCAGAG TGAAAAATAT CGGGTATGAA AGTGCCAGTG TGTCTAGCAC ATGTGAACCT 
TGCAAAAGTA GAAACAGACA TTCAGCCCAA ACTGAAGAGC CTGTTCAAGC AAAAGTACTC 
AGCAGAAAGA ATCATGAGCA GCTGGAAAAA GTCATAAGAT GTAGTAGGTC TACAGAAATA 
TCTTCAGCAC ATGCTAGGAG AATACTACAG CAGTCTAACA GAAATGCATG CAATGAAGCG 
CCAGAAACCG GGAGTGATTT TTCCATGTTC GAAGCTTTGC GGGATACCAT TTATTCTGAA 
GTAGCTACGT TGATTTCTCA AAATGAATCT CGTCCACATT TTCTTATTGA ACTCTTCCAT 
GAGCTTCAGC TACTTAATAC AGACTATTTG AGACAGAGGG CCTTATATGC ATTGCAGGAC 
ATAGTATCCA GGCATATTTC TGAGAACCAT GAAAAAGAAG GAGAAAATAT AAAGTCCGTG 
AATTCTGGTA CTTGGATAGC ATCCAACTCA GAACTTACTC CCAGTGAAAG CCTTGCTACT 
ACTGATGATG AAACTTTTGA GAAGAACTTT GAGAGAGAAA CACATAAAAT AAGTGAGCAA 
AATGATGCTG ATAATGCTAG TGTATCTTCA ACTTGTGAGC CTTTTGCAAC AGATGTTCTG 
GGCGACACTG TGATTCACTT AGATCAGGCA TTAGCCAGAA TGAGAGACTA TACGCATATG 
AAGATGGAGG CTGAAAGTAG TCCAGACGTC AGGTGCACCT GCAGGATCAT TGAGGATGAC 
GATGGTGCTG CTGCCACAAC CACGATTAGT AATAATTTAG AAGAAACTCC TGTTATTGAA 
AATCATAGTT CACAGCAACC CGTGAGTGAA GTTGCTACCG TCCCGTGTCC TAGAATTGAT 
ACTCAGCAAC TGGACCGGCA AATTAAAGCA ATTATGAAAG AAGTCATTCC TTTTTTGAAG 
GAGCACATGG ATGAAGTATG TTCTTCTCAA CTTCTAACCT CAGTAAGACG CATGGTTTTG 
GTCCTTACTC AGCAAAACAA TGAGAGCAAA GAGTTTGTAA AGTTCTTTCA TAAGCAGCTT 
GGAAGTATAT TACAGGATTC ACTCGCAAAA TTTGCTGGCA GAAAACTGAA GGATTGTGGA 
GAAGATCTTC TTGTAGAGAT ATCTGAAGTG TTATTTAATG AATTGGCTTT CTTTAAGCTC 
ATGCAAGATT TGGATAATAA TAGTGTAACT GTTAAACAGA GATGCAAAAG GAAAATAGAA 
GCTGCTGGAG TGATACAGTC TTATGCAAAA GAGGACAAGG ATGAGACGGA AACAGTTAAG 
CAGACTCAAA CGTCTGAGGT TTATGATGGT GACGGCCCCA AAAATGTGAG ATCTGATGTT 
TCTGATCAAG AGGATGATGA AGAAAGTGAA GAATGTCCAG TGTCTATTAA TTTGTCCAAA 
GCTGAAACTC AGGCTTTGAC TAATTACGGA AGTGGAGAAG ATGAGAATGA AGATGAAGAA 
ATAGAAGAAT TTGAAGAGGG ACCTGTAGAT GTCCAGACGT CACTTCAGGC TAACACTGAA 
ACTACAGAAG AAAATGAACA CGATGATCAG GTTCCACAGC ATGATTTTGA AAAATCATCA 
GAAAGCAAAA ATGTCCCATC AGAACAAGAA CCCACTACTA GTAAAGACTT TCTTTTGATG 
ACAGGTGACC AGAATAGCAC TCCTGTGAAG CCCTGTTACC TCAGTATCGT GGAAAATGAG 
CAACCTTTAA ATAGCCCTGT CCAGAAGGAC ACTCTCACTA CCATCGACTC ATCCAGTCAG 
CCTAACCCTT TGCCTTTACC TTTAACTGAA ATTGAAACCT TGGTGCCTAG AGTCAAAGAA 
GTGAAATCTG CTCAGGAGAC TCCTGAAAGT TCTTTGGCTG GAAGTCCTGA TACTGAATCT 
CCAGTGTTAG TGAATGACTA TGAAGCAGAA TCTGGTAACA TAAGTCAAAA ATCTGATGAA 
GAAGACTTTG TGAAAGTTGA AGATTTACCA CTTAAACTGA CAATATATTC AGAGGCAGAT 
CTAAGGAAGA AAATGGCCGA AGAAGAACAG AAAAACCACT TATCTGGTGA AATATGTGCA 
ATGCAGACTG AAGAATTAGC TGGGAATTCT CAGACACTAA AAGAACCTGA GCAAGTATGT 
GCTCAGTCTG CCATCTTAAG ATGGAAACCC TTACCTGTGC CTTTTAAACT GTTGGTGGTG 
TCATCAGGGA ATTATTGTAG CAAAAAACAG TGGGAGCCCA AAGTACATGA GATGTCTCCA 
GAGGCTCAGC TAACTCTTTC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( XM_025279455.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025135240.1 Bubalus bubalis pericentriolar material 1 (PCM1), transcript variant X8, mRNA.

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CloneID	OBu42296
Clone ID Related Accession (Same CDS sequence)	XM_025279455.1
Accession Version	XM_025279455.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6588bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	pericentriolar material 1 protein isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037545.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG GAGGAGGTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
AGCAATGAGA GTGTTGATGA CCGACTCAAC AATATGGATT GGGGCAGCCA GCAGAAGAAG 
GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA 
ACTAACGATA TTTCTCCGGA GTCATCACCG GGAGTTGGAA GACGAAGAAC AAAGACTTCA 
CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TCCCCGAGCA GGCAGAACTA 
GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGATTAATAC TAACAAGAGC 
AAAGATGCCA CTGTAAGTCC TCAAAAGAGA GAAATGATTG GATCAGCACA GTGTAAAGAG 
TTGTTTGCTT CTGCTTTAAG TAATGACCTT TTGCAAAACT GTCAAGTCTC TGAAGAAGAT 
GGAAGAGGAG AACCTGCAAT GGAGAGCAGC CAGATTGTAA GCAGGCTTGT TCAGATTCGT 
GAGTATATTA CTAAAGCTAG TTCCATGCGG GAAGACCTTG TAGAGAAAAA TGAAAGATCT 
GCTAATGTTG AGCGCCTCAC TCATCTAATA GATCACCTTA AAGAACAAGA AAAGTCATAT 
ATGAAATTTC TCCAGAAGAT TCTTGCTAGA GAAAATGAGG AGGAGGATGT TCGGACTATA 
GATTCAGCTG TGGGATCTGG TTCTGTAGCT GAGAGCACAT CGCTAAACAT AGATGTGCGG 
TCTGAGGCTT CAGATGCCAC GGAGGCATCT TTTAGTCTGA GAATTCGGCC GTGCGTTGAG 
GACAAACTAG GGAATTCAGC TTCACAAGCA CAGGTTTCAG ACACTGACGT TACTACAAGT 
CCAAAAGGGA AAGGTGACAG ACCTCCTCAG CATGACAGGG AGCTGAGGCC TAATAGGAAA 
TGTAGCCGAA AACGTGGATT TCCCTCAAAG GCCAGAGATC CTCAGCAGGA GCCTATGGAA 
GAGATAGAAA ATTTGAAGAA ACAACATGAT TTATTAAAAA GGATGTTACA GCAGCAGGAG 
CAGCTGAGAG CTCTACAAGG ACGACAAGCT GCTCTGCTTG CCCTGCAGCA TAAAGCTGAG 
CAGGCTATAG CTGTGATGGA TGATTCTGAA AAGGTTGCGG AGACAACTCC TGGCCATCAT 
ACTGTACCAG GCAGACAGCC GGCTCATTCA CCTTTGCATC AGAGAGTACC CATAAGAGAA 
ACTACAGGTA GCCTATCTGG AGTCAGTATC ACCTCTGAAC TAAACGAAGA ATTGAATGAT 
TTAATTCAGC ATTTTCATAA TCAGCTTCGT GATTCTCAGC CTCCAGCTGT TCCAGACAAT 
AGAAGACAAG CAGAAAGTCT TTCATTAACT AGGGAGATTT CTCAGAGCAG AAATCCTTCA 
GTTTCAGAGC ATTTACCTGA TGAGAAAGTC CAACTTTTTA GCAAGATGAG AGTGTTACAA 
GAAAAGAAAC AAAAAATGGA CAAACTTCTT GGAGAACTTC ATACACTTCG AGATCAGCAT 
TTGAACAATT CATCATTTGT GCCTTCTTCA GCCTCTCCAC AAAGGAGTGT GGATCAGAGA 
AGTACAACCA CAGCTCCTTC TGCTCCTGTA GTCTTAACAC CAGTGGTCAA TGGAGAATCC 
AATAACCTCA CGCCATCTGT TCCTTATCCT GCTGCTTCTC TAGTTTCTCA GAATCAGAAT 
GAAAATGAAG GCCACCTAAA TCCAACTGAA AAACTCCAGA AGTTAAATGA AGTTCGAAAG 
AGGTTGAATG AACTAAGAGA ATTAGTTCAT TATTATGAGC AAACATCAGA TATGATGACA 
GATGCTGTGA ATGAAAACAC AAAAGAAGAA GAAACTGAAG AATCAGAATA TGATTCGGAG 
CATGAAAACT CGGAACCTGT TACTAACATT CGAAATCCAC AAGTGGCTGC CACCTGGAAC 
GAAGTAAATA GTAATTCTAA TGCACAGTGT GTTTCCAACA ATAGAGATGG GCGAGCAGTT 
AATTCTAACT GTGAAATTAA CAACAGGTCT GCTGCCAACA TAAGGGCTCT AAACATGCCT 
CCTCTTTCAG CAGATTGTCG ATACAATAGA GAAGGAGAAC AGGAGATGCC TGTTGCACAA 
GGTCATGAGG AGGAGGAGGA GGAGGAGGAG GAAGCAGAAG ATGAGGCCCT CAGTGGAGCT 
TCACTGTCTA GTCACAGCAG CAGCCTGATC ATTGAGACTC CTGAAGATGC TGAATTTGAA 
CATAAGATCC AGAAGCTTAT GGCTGCAAAA CTGAAACTTA GACACTTACA GAACCTTGTA 
GCCATGGTGC AGGACGATGA TGCAGCCAAT CAAGGAGTTA CTTCTGTGGA GGATTTCTTC 
CCAGCAGAAG ACACCAAACA AAATGCAAAT AACACTAGAG GAAATGCCAA TAAAACACAG 
AAAGATGCTG GAGTAAATGA AAAGGCAAGG GAGAAATTTT ATGAGGCTAA ACTACAGCAG 
CAACAGAGAG AACTCAAACA ATTGCAGGAA GAAAGAAAGA AACTGATTGA GATTCAAGAG 
AAAATTCAAG AATTGCAAAA GGCATGTCCT AACCTACAGC TGTCAGCTGC TAGTGTGGGT 
AATTGTCCCA CAAAAACACA TATGCCAGCT GTTACTTCAT CCCCAACCGT TAATGAAAAT 
GAAGCCAGTA CAAACAAATC TGGTTTTGAG CCTCATGATT CTTCAGTGGT AGATAATGAG 
GTATGGTCAG ATATGCGAAG ACATGAAATG TTAAGGGAGG AGCTACGACA GAGAAGAAAG 
CAGCTGGAAG CTCTGATGGC TGAACATCAG AGGAGGCAAG GTCTGGCTGA AACTGCATCT 
CCAGTGGCTG TTTCACTGAG AAGTGATGGA TCTGAGAACC TGTGTACACC TCAGCAAAGC 
AGAACAGAAA AAACAATGGC AACTTGGGGA GGTTCTACCC AGTGTGCCCT AGATGAAGAA 
GGAGATGAAG ATGGTTACCT TTCTGAAGGA ATTATTCGGA CAGACGAAGA GGAGGAAGAG 
GAGGAGCAAG ATGCCAGTTC CAATGATAAC TTTCATATGT ATCCTCCAAA CAGTGCAAAT 
CATAATTCTT ACAATGTAAA GGAAACTAAA AATAGGTGGA AGAACAATCG CCCTTTTTCA 
GCAGATGGAA ATTATCGTCC TTTAGCCAAG ACAAGACAGC AGAATATCAG CATGCAGCGT 
CAGGAAAACC TTCGCTGGGT GTCCGAACTC TCTTATGTAG AAGAGAAAGA ACAATGGCAA 
GAACAAATTA ATCAGCTAAA GAAACAACTT GATTTCAGTG TCAATATTTG TCAGACTTTA 
ATGCAAGACC AGCAGACTCT GTCTTGTCTG CTACAAACTC TTCTCACGAG TCCTTATAGT 
GTTATGCCCA GCAGTGTTGC ATCCCCGCAA GTGCACCTTA TAATGCACCA GTTGAATCAG 
TGCTATACCC AGCTAACGTG GCAGCAGAAC AATGTCCAGA GGTTGAAACA AATGCTCAGT 
GAACTCATGC GCCAACAAAA TCAGCATCCA GAAAAACCTG CAAGCAAGGA AGGAGGCAGT 
GGTACATCAC ACCCTCCTTC TCCCAGTTTA TTTTGTCCTT TCAACTTTCC AACTCAGCCT 
GTAAATCTGT TTAACCTACC GGGGTTTACT AATTTTTCGT CATTTGCACC AAGTATGAAT 
TTCAGCCCTT TATTTCCTTC TAATTTTGGA GATTTTTCTC AAAATATTTC TACACCCACT 
GAACAGCAGC AACCGTTAGC CCAGAATCCT TCAGGGAAAA CGGAATACAT GGCTTTTCCA 
AAACCTTTTG AAAGTAGTTC TTCTATTGGA GCGGAAAAAC AAAGGAATCA AAAACAGCCT 
GAAGAGGAGG TGGAGAACAG TAGGACACCA TGGTTTTATG ACCAAGAAGG TGAAGTAGAA 
AAGCCATTTC TCAAGACTGG ATTTGCAGTG TCTGTAGAGA AAACAACAAA TAGTAATCGC 
AAAAATCAAC TAGATACCAG TAGGAGAAGA CGCCAGTTTG ATGAAGTGTC CTTAGAAAGC 
TTTAACAGTA TGCTTGACCC AGTAGATCCA ACAACAGTAA CTAAAACATT CAAGTCAAGA 
AAAGCATCTG CACAGGCCAG CCTGGCATCT AAAGATAAAA CTCCCAAATC AAAGAGTAAG 
AAGAGGCATT CTACTCAGCT GAAAAGCAGA GTGAAAAATA TCGGGTATGA AAGTGCCAGT 
GTGTCTAGCA CATGTGAACC TTGCAAAAGT AGAAACAGAC ATTCAGCCCA AACTGAAGAG 
CCTGTTCAAG CAAAAGTACT CAGCAGAAAG AATCATGAGC AGCTGGAAAA AGTCATAAGA 
TGTAGTAGGT CTACAGAAAT ATCTTCAGCA CATGCTAGGA GAATACTACA GCAGTCTAAC 
AGAAATGCAT GCAATGAAGC GCCAGAAACC GGGAGTGATT TTTCCATGTT CGAAGCTTTG 
CGGGATACCA TTTATTCTGA AGTAGCTACG TTGATTTCTC AAAATGAATC TCGTCCACAT 
TTTCTTATTG AACTCTTCCA TGAGCTTCAG CTACTTAATA CAGACTATTT GAGACAGAGG 
GCCTTATATG CATTGCAGGA CATAGTATCC AGGCATATTT CTGAGAACCA TGAAAAAGAA 
GGAGAAAATA TAAAGTCCGT GAATTCTGGT ACTTGGATAG CATCCAACTC AGAACTTACT 
CCCAGTGAAA GCCTTGCTAC TACTGATGAT GAAACTTTTG AGAAGAACTT TGAGAGAGAA 
ACACATAAAA TAAGTGAGCA AAATGATGCT GATAATGCTA GTGTATCTTC AACTTGTGAG 
CCTTTTGCAA CAGATGTTCT GGGCGACACT GTGATTCACT TAGATCAGGC ATTAGCCAGA 
ATGAGAGACT ATACGCATAT GAAGATGGAG GCTGAAAGTA GTCCAGACGT CAGGTGCACC 
TGCAGGATCA TTGAGGATGA CGATGGTGCT GCTGCCACAA CCACGATTAG TAATAATTTA 
GAAGAAACTC CTGTTATTGA AAATCATAGT TCACAGCAAC CCGTGAGTGA AGTTGCTACC 
GTCCCGTGTC CTAGAATTGA TACTCAGCAA CTGGACCGGC AAATTAAAGC AATTATGAAA 
GAAGTCATTC CTTTTTTGAA GGAGCACATG GATGAAGTAT GTTCTTCTCA ACTTCTAACC 
TCAGTAAGAC GCATGGTTTT GGTCCTTACT CAGCAAAACA ATGAGAGCAA AGAGTTTGTA 
AAGTTCTTTC ATAAGCAGCT TGGAAGTATA TTACAGGATT CACTCGCAAA ATTTGCTGGC 
AGAAAACTGA AGGATTGTGG AGAAGATCTT CTTGTAGAGA TATCTGAAGT GTTATTTAAT 
GAATTGGCTT TCTTTAAGCT CATGCAAGAT TTGGATAATA ATAGTGTAAC TGTTAAACAG 
AGATGCAAAA GGAAAATAGA AGCTGCTGGA GTGATACAGT CTTATGCAAA AGAGGCCAAA 
AGGATTCTTG AAGGAGATCA CAGTTCACCT GCTGGAGAAA TTGATGATGA AGACAAAGAC 
AAGGATGAGA CGGAAACAGT TAAGCAGACT CAAACGTCTG AGGTTTATGA TGGTGACGGC 
CCCAAAAATG TGAGATCTGA TGTTTCTGAT CAAGAGGATG ATGAAGAAAG TGAAGAATGT 
CCAGTGTCTA TTAATTTGTC CAAAGCTGAA ACTCAGGCTT TGACTAATTA CGGAAGTGGA 
GAAGATGAGA ATGAAGATGA AGAAATAGAA GAATTTGAAG AGGGACCTGT AGATGTCCAG 
ACGTCACTTC AGGCTAACAC TGAAACTACA GAAGAAAATG AACACGATGA TCAGGTTCCA 
CAGCATGATT TTGAAAAATC ATCAGAAAGC AAAAATGTCC CATCAGAACA AGAACCCACT 
ACTAGTAAAG ACTTTCTTTT GATGACAGGT GACCAGAATA GCACTCCTGT GAAGCCCTGT 
TACCTCAGTA TCGTGGAAAA TGAGCAACCT TTAAATAGCC CTGTCCAGAA GGACACTCTC 
ACTACCATCG ACTCATCCAG TCAGCCTAAC CCTTTGCCTT TACCTTTAAC TGAAATTGAA 
ACCTTGGTGC CTAGAGTCAA AGAAGTGAAA TCTGCTCAGG AGACTCCTGA AAGTTCTTTG 
GCTGGAAGTC CTGATACTGA ATCTCCAGTG TTAGTGAATG ACTATGAAGC AGAATCTGGT 
AACATAAGTC AAAAATCTGA TGAAGAAGAC TTTGTGAAAG TTGAAGATTT ACCACTTAAA 
CTGACAATAT ATTCAGAGGC AGATCTAAGG AAGAAAATGG CCGAAGAAGA ACAGAAAAAC 
CACTTATCTG GTGAAATATG TGCAATGCAG ACTGAAGAAT TAGCTGGGAA TTCTCAGACA 
CTAAAAGAAC CTGAGCAAGT ATGTGCTCAG TCTGCCATCT TAAGATGGAA ACCCTTACCT 
GTGCCTTTTA AACTGTTGGT GGTGTCATCA GGGAATTATT GTAGCAAAAA ACAGTGGGAG 
CCCAAAGTAC ATGAGATGTC TCCAGAGGCT CAGCTAACTC TTTCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025135240.1
CDS	392..6979
Translation	MATGGGPFEEGVNDQDLPNWSNESVDDRLNNMDWGSQQKKANRSSEKNKRKFGVESDKRVTNDISPESSPGVGRRRTKTSLTFPHSRYMTQMSVPEQAELEKLKQRINFSDLDQINTNKSKDATVSPQKREMIGSAQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIREYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLQKILARENEEEDVRTIDSAVGSGSVAESTSLNIDVRSEASDATEASFSLRIRPCVEDKLGNSASQAQVSDTDVTTSPKGKGDRPPQHDRELRPNRKCSRKRGFPSKARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSEKVAETTPGHHTVPGRQPAHSPLHQRVPIRETTGSLSGVSITSELNEELNDLIQHFHNQLRDSQPPAVPDNRRQAESLSLTREISQSRNPSVSEHLPDEKVQLFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSFVPSSASPQRSVDQRSTTTAPSAPVVLTPVVNGESNNLTPSVPYPAASLVSQNQNENEGHLNPTEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENTKEEETEESEYDSEHENSEPVTNIRNPQVAATWNEVNSNSNAQCVSNNRDGRAVNSNCEINNRSAANIRALNMPPLSADCRYNREGEQEMPVAQGHEEEEEEEEEAEDEALSGASLSSHSSSLIIETPEDAEFEHKIQKLMAAKLKLRHLQNLVAMVQDDDAANQGVTSVEDFFPAEDTKQNANNTRGNANKTQKDAGVNEKAREKFYEAKLQQQQRELKQLQEERKKLIEIQEKIQELQKACPNLQLSAASVGNCPTKTHMPAVTSSPTVNENEASTNKSGFEPHDSSVVDNEVWSDMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETASPVAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGIIRTDEEEEEEEQDASSNDNFHMYPPNSANHNSYNVKETKNRWKNNRPFSADGNYRPLAKTRQQNISMQRQENLRWVSELSYVEEKEQWQEQINQLKKQLDFSVNICQTLMQDQQTLSCLLQTLLTSPYSVMPSSVASPQVHLIMHQLNQCYTQLTWQQNNVQRLKQMLSELMRQQNQHPEKPASKEGGSGTSHPPSPSLFCPFNFPTQPVNLFNLPGFTNFSSFAPSMNFSPLFPSNFGDFSQNISTPTEQQQPLAQNPSGKTEYMAFPKPFESSSSIGAEKQRNQKQPEEEVENSRTPWFYDQEGEVEKPFLKTGFAVSVEKTTNSNRKNQLDTSRRRRQFDEVSLESFNSMLDPVDPTTVTKTFKSRKASAQASLASKDKTPKSKSKKRHSTQLKSRVKNIGYESASVSSTCEPCKSRNRHSAQTEEPVQAKVLSRKNHEQLEKVIRCSRSTEISSAHARRILQQSNRNACNEAPETGSDFSMFEALRDTIYSEVATLISQNESRPHFLIELFHELQLLNTDYLRQRALYALQDIVSRHISENHEKEGENIKSVNSGTWIASNSELTPSESLATTDDETFEKNFERETHKISEQNDADNASVSSTCEPFATDVLGDTVIHLDQALARMRDYTHMKMEAESSPDVRCTCRIIEDDDGAAATTTISNNLEETPVIENHSSQQPVSEVATVPCPRIDTQQLDRQIKAIMKEVIPFLKEHMDEVCSSQLLTSVRRMVLVLTQQNNESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSVTVKQRCKRKIEAAGVIQSYAKEAKRILEGDHSSPAGEIDDEDKDKDETETVKQTQTSEVYDGDGPKNVRSDVSDQEDDEESEECPVSINLSKAETQALTNYGSGEDENEDEEIEEFEEGPVDVQTSLQANTETTEENEHDDQVPQHDFEKSSESKNVPSEQEPTTSKDFLLMTGDQNSTPVKPCYLSIVENEQPLNSPVQKDTLTTIDSSSQPNPLPLPLTEIETLVPRVKEVKSAQETPESSLAGSPDTESPVLVNDYEAESGNISQKSDEEDFVKVEDLPLKLTIYSEADLRKKMAEEEQKNHLSGEICAMQTEELAGNSQTLKEPEQVCAQSAILRWKPLPVPFKLLVVSSGNYCSKKQWEPKVHEMSPEAQLTLS

Target ORF information:

RefSeq Version	XM_025279455.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis pericentriolar material 1 (PCM1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025279455.1

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
AGCAATGAGA GTGTTGATGA CCGACTCAAC AATATGGATT GGGGCAGCCA GCAGAAGAAG 
GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA 
ACTAACGATA TTTCTCCGGA GTCATCACCG GGAGTTGGAA GACGAAGAAC AAAGACTTCA 
CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TCCCCGAGCA GGCAGAACTA 
GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGATTAATAC TAACAAGAGC 
AAAGATGCCA CTGTAAGTCC TCAAAAGAGA GAAATGATTG GATCAGCACA GTGTAAAGAG 
TTGTTTGCTT CTGCTTTAAG TAATGACCTT TTGCAAAACT GTCAAGTCTC TGAAGAAGAT 
GGAAGAGGAG AACCTGCAAT GGAGAGCAGC CAGATTGTAA GCAGGCTTGT TCAGATTCGT 
GAGTATATTA CTAAAGCTAG TTCCATGCGG GAAGACCTTG TAGAGAAAAA TGAAAGATCT 
GCTAATGTTG AGCGCCTCAC TCATCTAATA GATCACCTTA AAGAACAAGA AAAGTCATAT 
ATGAAATTTC TCCAGAAGAT TCTTGCTAGA GAAAATGAGG AGGAGGATGT TCGGACTATA 
GATTCAGCTG TGGGATCTGG TTCTGTAGCT GAGAGCACAT CGCTAAACAT AGATGTGCGG 
TCTGAGGCTT CAGATGCCAC GGAGGCATCT TTTAGTCTGA GAATTCGGCC GTGCGTTGAG 
GACAAACTAG GGAATTCAGC TTCACAAGCA CAGGTTTCAG ACACTGACGT TACTACAAGT 
CCAAAAGGGA AAGGTGACAG ACCTCCTCAG CATGACAGGG AGCTGAGGCC TAATAGGAAA 
TGTAGCCGAA AACGTGGATT TCCCTCAAAG GCCAGAGATC CTCAGCAGGA GCCTATGGAA 
GAGATAGAAA ATTTGAAGAA ACAACATGAT TTATTAAAAA GGATGTTACA GCAGCAGGAG 
CAGCTGAGAG CTCTACAAGG ACGACAAGCT GCTCTGCTTG CCCTGCAGCA TAAAGCTGAG 
CAGGCTATAG CTGTGATGGA TGATTCTGAA AAGGTTGCGG AGACAACTCC TGGCCATCAT 
ACTGTACCAG GCAGACAGCC GGCTCATTCA CCTTTGCATC AGAGAGTACC CATAAGAGAA 
ACTACAGGTA GCCTATCTGG AGTCAGTATC ACCTCTGAAC TAAACGAAGA ATTGAATGAT 
TTAATTCAGC ATTTTCATAA TCAGCTTCGT GATTCTCAGC CTCCAGCTGT TCCAGACAAT 
AGAAGACAAG CAGAAAGTCT TTCATTAACT AGGGAGATTT CTCAGAGCAG AAATCCTTCA 
GTTTCAGAGC ATTTACCTGA TGAGAAAGTC CAACTTTTTA GCAAGATGAG AGTGTTACAA 
GAAAAGAAAC AAAAAATGGA CAAACTTCTT GGAGAACTTC ATACACTTCG AGATCAGCAT 
TTGAACAATT CATCATTTGT GCCTTCTTCA GCCTCTCCAC AAAGGAGTGT GGATCAGAGA 
AGTACAACCA CAGCTCCTTC TGCTCCTGTA GTCTTAACAC CAGTGGTCAA TGGAGAATCC 
AATAACCTCA CGCCATCTGT TCCTTATCCT GCTGCTTCTC TAGTTTCTCA GAATCAGAAT 
GAAAATGAAG GCCACCTAAA TCCAACTGAA AAACTCCAGA AGTTAAATGA AGTTCGAAAG 
AGGTTGAATG AACTAAGAGA ATTAGTTCAT TATTATGAGC AAACATCAGA TATGATGACA 
GATGCTGTGA ATGAAAACAC AAAAGAAGAA GAAACTGAAG AATCAGAATA TGATTCGGAG 
CATGAAAACT CGGAACCTGT TACTAACATT CGAAATCCAC AAGTGGCTGC CACCTGGAAC 
GAAGTAAATA GTAATTCTAA TGCACAGTGT GTTTCCAACA ATAGAGATGG GCGAGCAGTT 
AATTCTAACT GTGAAATTAA CAACAGGTCT GCTGCCAACA TAAGGGCTCT AAACATGCCT 
CCTCTTTCAG CAGATTGTCG ATACAATAGA GAAGGAGAAC AGGAGATGCC TGTTGCACAA 
GGTCATGAGG AGGAGGAGGA GGAGGAGGAG GAAGCAGAAG ATGAGGCCCT CAGTGGAGCT 
TCACTGTCTA GTCACAGCAG CAGCCTGATC ATTGAGACTC CTGAAGATGC TGAATTTGAA 
CATAAGATCC AGAAGCTTAT GGCTGCAAAA CTGAAACTTA GACACTTACA GAACCTTGTA 
GCCATGGTGC AGGACGATGA TGCAGCCAAT CAAGGAGTTA CTTCTGTGGA GGATTTCTTC 
CCAGCAGAAG ACACCAAACA AAATGCAAAT AACACTAGAG GAAATGCCAA TAAAACACAG 
AAAGATGCTG GAGTAAATGA AAAGGCAAGG GAGAAATTTT ATGAGGCTAA ACTACAGCAG 
CAACAGAGAG AACTCAAACA ATTGCAGGAA GAAAGAAAGA AACTGATTGA GATTCAAGAG 
AAAATTCAAG AATTGCAAAA GGCATGTCCT AACCTACAGC TGTCAGCTGC TAGTGTGGGT 
AATTGTCCCA CAAAAACACA TATGCCAGCT GTTACTTCAT CCCCAACCGT TAATGAAAAT 
GAAGCCAGTA CAAACAAATC TGGTTTTGAG CCTCATGATT CTTCAGTGGT AGATAATGAG 
GTATGGTCAG ATATGCGAAG ACATGAAATG TTAAGGGAGG AGCTACGACA GAGAAGAAAG 
CAGCTGGAAG CTCTGATGGC TGAACATCAG AGGAGGCAAG GTCTGGCTGA AACTGCATCT 
CCAGTGGCTG TTTCACTGAG AAGTGATGGA TCTGAGAACC TGTGTACACC TCAGCAAAGC 
AGAACAGAAA AAACAATGGC AACTTGGGGA GGTTCTACCC AGTGTGCCCT AGATGAAGAA 
GGAGATGAAG ATGGTTACCT TTCTGAAGGA ATTATTCGGA CAGACGAAGA GGAGGAAGAG 
GAGGAGCAAG ATGCCAGTTC CAATGATAAC TTTCATATGT ATCCTCCAAA CAGTGCAAAT 
CATAATTCTT ACAATGTAAA GGAAACTAAA AATAGGTGGA AGAACAATCG CCCTTTTTCA 
GCAGATGGAA ATTATCGTCC TTTAGCCAAG ACAAGACAGC AGAATATCAG CATGCAGCGT 
CAGGAAAACC TTCGCTGGGT GTCCGAACTC TCTTATGTAG AAGAGAAAGA ACAATGGCAA 
GAACAAATTA ATCAGCTAAA GAAACAACTT GATTTCAGTG TCAATATTTG TCAGACTTTA 
ATGCAAGACC AGCAGACTCT GTCTTGTCTG CTACAAACTC TTCTCACGAG TCCTTATAGT 
GTTATGCCCA GCAGTGTTGC ATCCCCGCAA GTGCACCTTA TAATGCACCA GTTGAATCAG 
TGCTATACCC AGCTAACGTG GCAGCAGAAC AATGTCCAGA GGTTGAAACA AATGCTCAGT 
GAACTCATGC GCCAACAAAA TCAGCATCCA GAAAAACCTG CAAGCAAGGA AGGAGGCAGT 
GGTACATCAC ACCCTCCTTC TCCCAGTTTA TTTTGTCCTT TCAACTTTCC AACTCAGCCT 
GTAAATCTGT TTAACCTACC GGGGTTTACT AATTTTTCGT CATTTGCACC AAGTATGAAT 
TTCAGCCCTT TATTTCCTTC TAATTTTGGA GATTTTTCTC AAAATATTTC TACACCCACT 
GAACAGCAGC AACCGTTAGC CCAGAATCCT TCAGGGAAAA CGGAATACAT GGCTTTTCCA 
AAACCTTTTG AAAGTAGTTC TTCTATTGGA GCGGAAAAAC AAAGGAATCA AAAACAGCCT 
GAAGAGGAGG TGGAGAACAG TAGGACACCA TGGTTTTATG ACCAAGAAGG TGAAGTAGAA 
AAGCCATTTC TCAAGACTGG ATTTGCAGTG TCTGTAGAGA AAACAACAAA TAGTAATCGC 
AAAAATCAAC TAGATACCAG TAGGAGAAGA CGCCAGTTTG ATGAAGTGTC CTTAGAAAGC 
TTTAACAGTA TGCTTGACCC AGTAGATCCA ACAACAGTAA CTAAAACATT CAAGTCAAGA 
AAAGCATCTG CACAGGCCAG CCTGGCATCT AAAGATAAAA CTCCCAAATC AAAGAGTAAG 
AAGAGGCATT CTACTCAGCT GAAAAGCAGA GTGAAAAATA TCGGGTATGA AAGTGCCAGT 
GTGTCTAGCA CATGTGAACC TTGCAAAAGT AGAAACAGAC ATTCAGCCCA AACTGAAGAG 
CCTGTTCAAG CAAAAGTACT CAGCAGAAAG AATCATGAGC AGCTGGAAAA AGTCATAAGA 
TGTAGTAGGT CTACAGAAAT ATCTTCAGCA CATGCTAGGA GAATACTACA GCAGTCTAAC 
AGAAATGCAT GCAATGAAGC GCCAGAAACC GGGAGTGATT TTTCCATGTT CGAAGCTTTG 
CGGGATACCA TTTATTCTGA AGTAGCTACG TTGATTTCTC AAAATGAATC TCGTCCACAT 
TTTCTTATTG AACTCTTCCA TGAGCTTCAG CTACTTAATA CAGACTATTT GAGACAGAGG 
GCCTTATATG CATTGCAGGA CATAGTATCC AGGCATATTT CTGAGAACCA TGAAAAAGAA 
GGAGAAAATA TAAAGTCCGT GAATTCTGGT ACTTGGATAG CATCCAACTC AGAACTTACT 
CCCAGTGAAA GCCTTGCTAC TACTGATGAT GAAACTTTTG AGAAGAACTT TGAGAGAGAA 
ACACATAAAA TAAGTGAGCA AAATGATGCT GATAATGCTA GTGTATCTTC AACTTGTGAG 
CCTTTTGCAA CAGATGTTCT GGGCGACACT GTGATTCACT TAGATCAGGC ATTAGCCAGA 
ATGAGAGACT ATACGCATAT GAAGATGGAG GCTGAAAGTA GTCCAGACGT CAGGTGCACC 
TGCAGGATCA TTGAGGATGA CGATGGTGCT GCTGCCACAA CCACGATTAG TAATAATTTA 
GAAGAAACTC CTGTTATTGA AAATCATAGT TCACAGCAAC CCGTGAGTGA AGTTGCTACC 
GTCCCGTGTC CTAGAATTGA TACTCAGCAA CTGGACCGGC AAATTAAAGC AATTATGAAA 
GAAGTCATTC CTTTTTTGAA GGAGCACATG GATGAAGTAT GTTCTTCTCA ACTTCTAACC 
TCAGTAAGAC GCATGGTTTT GGTCCTTACT CAGCAAAACA ATGAGAGCAA AGAGTTTGTA 
AAGTTCTTTC ATAAGCAGCT TGGAAGTATA TTACAGGATT CACTCGCAAA ATTTGCTGGC 
AGAAAACTGA AGGATTGTGG AGAAGATCTT CTTGTAGAGA TATCTGAAGT GTTATTTAAT 
GAATTGGCTT TCTTTAAGCT CATGCAAGAT TTGGATAATA ATAGTGTAAC TGTTAAACAG 
AGATGCAAAA GGAAAATAGA AGCTGCTGGA GTGATACAGT CTTATGCAAA AGAGGCCAAA 
AGGATTCTTG AAGGAGATCA CAGTTCACCT GCTGGAGAAA TTGATGATGA AGACAAAGAC 
AAGGATGAGA CGGAAACAGT TAAGCAGACT CAAACGTCTG AGGTTTATGA TGGTGACGGC 
CCCAAAAATG TGAGATCTGA TGTTTCTGAT CAAGAGGATG ATGAAGAAAG TGAAGAATGT 
CCAGTGTCTA TTAATTTGTC CAAAGCTGAA ACTCAGGCTT TGACTAATTA CGGAAGTGGA 
GAAGATGAGA ATGAAGATGA AGAAATAGAA GAATTTGAAG AGGGACCTGT AGATGTCCAG 
ACGTCACTTC AGGCTAACAC TGAAACTACA GAAGAAAATG AACACGATGA TCAGGTTCCA 
CAGCATGATT TTGAAAAATC ATCAGAAAGC AAAAATGTCC CATCAGAACA AGAACCCACT 
ACTAGTAAAG ACTTTCTTTT GATGACAGGT GACCAGAATA GCACTCCTGT GAAGCCCTGT 
TACCTCAGTA TCGTGGAAAA TGAGCAACCT TTAAATAGCC CTGTCCAGAA GGACACTCTC 
ACTACCATCG ACTCATCCAG TCAGCCTAAC CCTTTGCCTT TACCTTTAAC TGAAATTGAA 
ACCTTGGTGC CTAGAGTCAA AGAAGTGAAA TCTGCTCAGG AGACTCCTGA AAGTTCTTTG 
GCTGGAAGTC CTGATACTGA ATCTCCAGTG TTAGTGAATG ACTATGAAGC AGAATCTGGT 
AACATAAGTC AAAAATCTGA TGAAGAAGAC TTTGTGAAAG TTGAAGATTT ACCACTTAAA 
CTGACAATAT ATTCAGAGGC AGATCTAAGG AAGAAAATGG CCGAAGAAGA ACAGAAAAAC 
CACTTATCTG GTGAAATATG TGCAATGCAG ACTGAAGAAT TAGCTGGGAA TTCTCAGACA 
CTAAAAGAAC CTGAGCAAGT ATGTGCTCAG TCTGCCATCT TAAGATGGAA ACCCTTACCT 
GTGCCTTTTA AACTGTTGGT GGTGTCATCA GGGAATTATT GTAGCAAAAA ACAGTGGGAG 
CCCAAAGTAC ATGAGATGTC TCCAGAGGCT CAGCTAACTC TTTCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( XM_025279460.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025135245.1 Bubalus bubalis pericentriolar material 1 (PCM1), transcript variant X9, mRNA.

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CloneID	OBu42297
Clone ID Related Accession (Same CDS sequence)	XM_025279460.1
Accession Version	XM_025279460.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6558bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	pericentriolar material 1 protein isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037545.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG GAGGAGGTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
AGCAATGAGA GTGTTGATGA CCGACTCAAC AATATGGATT GGGGCAGCCA GCAGAAGAAG 
GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA 
ACTAACGATA TTTCTCCGGA GTCATCACCG GGAGTTGGAA GACGAAGAAC AAAGACTTCA 
CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TCCCCGAGCA GGCAGAACTA 
GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCTCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCCAC TGTAAGTCCT 
CAAAAGAGAG AAATGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAGATTCGTG AGTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGACCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTCACT 
CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT CCAGAAGATT 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCGGT CTGAGGCTTC AGATGCCACG 
GAGGCATCTT TTAGTCTGAG AATTCGGCCG TGCGTTGAGG ACAAACTAGG GAATTCAGCT 
TCACAAGCAC AGGTTTCAGA CACTGACGTT ACTACAAGTC CAAAAGGGAA AGGTGACAGA 
CCTCCTCAGC ATGACAGGGA GCTGAGGCCT AATAGGAAAT GTAGCCGAAA ACGTGGATTT 
CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATGGAAG AGATAGAAAA TTTGAAGAAA 
CAACATGATT TATTAAAAAG GATGTTACAG CAGCAGGAGC AGCTGAGAGC TCTACAAGGA 
CGACAAGCTG CTCTGCTTGC CCTGCAGCAT AAAGCTGAGC AGGCTATAGC TGTGATGGAT 
GATTCTGAAA AGGTTGCGGA GACAACTCCT GGCCATCATA CTGTACCAGG CAGACAGCCG 
GCTCATTCAC CTTTGCATCA GAGAGTACCC ATAAGAGAAA CTACAGGTAG CCTATCTGGA 
GTCAGTATCA CCTCTGAACT AAACGAAGAA TTGAATGATT TAATTCAGCA TTTTCATAAT 
CAGCTTCGTG ATTCTCAGCC TCCAGCTGTT CCAGACAATA GAAGACAAGC AGAAAGTCTT 
TCATTAACTA GGGAGATTTC TCAGAGCAGA AATCCTTCAG TTTCAGAGCA TTTACCTGAT 
GAGAAAGTCC AACTTTTTAG CAAGATGAGA GTGTTACAAG AAAAGAAACA AAAAATGGAC 
AAACTTCTTG GAGAACTTCA TACACTTCGA GATCAGCATT TGAACAATTC ATCATTTGTG 
CCTTCTTCAG CCTCTCCACA AAGGAGTGTG GATCAGAGAA GTACAACCAC AGCTCCTTCT 
GCTCCTGTAG TCTTAACACC AGTGGTCAAT GGAGAATCCA ATAACCTCAC GCCATCTGTT 
CCTTATCCTG CTGCTTCTCT AGTTTCTCAG AATCAGAATG AAAATGAAGG CCACCTAAAT 
CCAACTGAAA AACTCCAGAA GTTAAATGAA GTTCGAAAGA GGTTGAATGA ACTAAGAGAA 
TTAGTTCATT ATTATGAGCA AACATCAGAT ATGATGACAG ATGCTGTGAA TGAAAACACA 
AAAGAAGAAG AAACTGAAGA ATCAGAATAT GATTCGGAGC ATGAAAACTC GGAACCTGTT 
ACTAACATTC GAAATCCACA AGTGGCTGCC ACCTGGAACG AAGTAAATAG TAATTCTAAT 
GCACAGTGTG TTTCCAACAA TAGAGATGGG CGAGCAGTTA ATTCTAACTG TGAAATTAAC 
AACAGGTCTG CTGCCAACAT AAGGGCTCTA AACATGCCTC CTCTTTCAGC AGATTGTCGA 
TACAATAGAG AAGGAGAACA GGAGATGCCT GTTGCACAAG GTCATGAGGA GGAGGAGGAG 
GAGGAGGAGG AAGCAGAAGA TGAGGCCCTC AGTGGAGCTT CACTGTCTAG TCACAGCAGC 
AGCCTGATCA TTGAGACTCC TGAAGATGCT GAATTTGAAC ATAAGATCCA GAAGCTTATG 
GCTGCAAAAC TGAAACTTAG ACACTTACAG AACCTTGTAG CCATGGTGCA GGACGATGAT 
GCAGCCAATC AAGGAGTTAC TTCTGTGGAG GATTTCTTCC CAGCAGAAGA CACCAAACAA 
AATGCAAATA ACACTAGAGG AAATGCCAAT AAAACACAGA AAGATGCTGG AGTAAATGAA 
AAGGCAAGGG AGAAATTTTA TGAGGCTAAA CTACAGCAGC AACAGAGAGA ACTCAAACAA 
TTGCAGGAAG AAAGAAAGAA ACTGATTGAG ATTCAAGAGA AAATTCAAGA ATTGCAAAAG 
GCATGTCCTA ACCTACAGCT GTCAGCTGCT AGTGTGGGTA ATTGTCCCAC AAAAACACAT 
ATGCCAGCTG TTACTTCATC CCCAACCGTT AATGAAAATG AAGCCAGTAC AAACAAATCT 
GGTTTTGAGC CTCATGATTC TTCAGTGGTA GATAATGAGG TATGGTCAGA TATGCGAAGA 
CATGAAATGT TAAGGGAGGA GCTACGACAG AGAAGAAAGC AGCTGGAAGC TCTGATGGCT 
GAACATCAGA GGAGGCAAGG TCTGGCTGAA ACTGCATCTC CAGTGGCTGT TTCACTGAGA 
AGTGATGGAT CTGAGAACCT GTGTACACCT CAGCAAAGCA GAACAGAAAA AACAATGGCA 
ACTTGGGGAG GTTCTACCCA GTGTGCCCTA GATGAAGAAG GAGATGAAGA TGGTTACCTT 
TCTGAAGGAA TTATTCGGAC AGACGAAGAG GAGGAAGAGG AGGAGCAAGA TGCCAGTTCC 
AATGATAACT TTCATATGTA TCCTCCAAAC AGTGCAAATC ATAATTCTTA CAATGTAAAG 
GAAACTAAAA ATAGGTGGAA GAACAATCGC CCTTTTTCAG CAGATGGAAA TTATCGTCCT 
TTAGCCAAGA CAAGACAGCA GAATATCAGC ATGCAGCGTC AGGAAAACCT TCGCTGGGTG 
TCCGAACTCT CTTATGTAGA AGAGAAAGAA CAATGGCAAG AACAAATTAA TCAGCTAAAG 
AAACAACTTG ATTTCAGTGT CAATATTTGT CAGACTTTAA TGCAAGACCA GCAGACTCTG 
TCTTGTCTGC TACAAACTCT TCTCACGAGT CCTTATAGTG TTATGCCCAG CAGTGTTGCA 
TCCCCGCAAG TGCACCTTAT AATGCACCAG TTGAATCAGT GCTATACCCA GCTAACGTGG 
CAGCAGAACA ATGTCCAGAG GTTGAAACAA ATGCTCAGTG AACTCATGCG CCAACAAAAT 
CAGCATCCAG AAAAACCTGC AAGCAAGGAA GGAGGCAGTG GTACATCACA CCCTCCTTCT 
CCCAGTTTAT TTTGTCCTTT CAACTTTCCA ACTCAGCCTG TAAATCTGTT TAACCTACCG 
GGGTTTACTA ATTTTTCGTC ATTTGCACCA AGTATGAATT TCAGCCCTTT ATTTCCTTCT 
AATTTTGGAG ATTTTTCTCA AAATATTTCT ACACCCACTG AACAGCAGCA ACCGTTAGCC 
CAGAATCCTT CAGGGAAAAC GGAATACATG GCTTTTCCAA AACCTTTTGA AAGTAGTTCT 
TCTATTGGAG CGGAAAAACA AAGGAATCAA AAACAGCCTG AAGAGGAGGT GGAGAACAGT 
AGGACACCAT GGTTTTATGA CCAAGAAGGT GAAGTAGAAA AGCCATTTCT CAAGACTGGA 
TTTGCAGTGT CTGTAGAGAA AACAACAAAT AGTAATCGCA AAAATCAACT AGATACCAGT 
AGGAGAAGAC GCCAGTTTGA TGAAGTGTCC TTAGAAAGCT TTAACAGTAT GCTTGACCCA 
GTAGATCCAA CAACAGTAAC TAAAACATTC AAGTCAAGAA AAGCATCTGC ACAGGCCAGC 
CTGGCATCTA AAGATAAAAC TCCCAAATCA AAGAGTAAGA AGAGGCATTC TACTCAGCTG 
AAAAGCAGAG TGAAAAATAT CGGGTATGAA AGTGCCAGTG TGTCTAGCAC ATGTGAACCT 
TGCAAAAGTA GAAACAGACA TTCAGCCCAA ACTGAAGAGC CTGTTCAAGC AAAAGTACTC 
AGCAGAAAGA ATCATGAGCA GCTGGAAAAA GTCATAAGAT GTAGTAGGTC TACAGAAATA 
TCTTCAGCAC ATGCTAGGAG AATACTACAG CAGTCTAACA GAAATGCATG CAATGAAGCG 
CCAGAAACCG GGAGTGATTT TTCCATGTTC GAAGCTTTGC GGGATACCAT TTATTCTGAA 
GTAGCTACGT TGATTTCTCA AAATGAATCT CGTCCACATT TTCTTATTGA ACTCTTCCAT 
GAGCTTCAGC TACTTAATAC AGACTATTTG AGACAGAGGG CCTTATATGC ATTGCAGGAC 
ATAGTATCCA GGCATATTTC TGAGAACCAT GAAAAAGAAG GAGAAAATAT AAAGTCCGTG 
AATTCTGGTA CTTGGATAGC ATCCAACTCA GAACTTACTC CCAGTGAAAG CCTTGCTACT 
ACTGATGATG AAACTTTTGA GAAGAACTTT GAGAGAGAAA CACATAAAAT AAGTGAGCAA 
AATGATGCTG ATAATGCTAG TGTATCTTCA ACTTGTGAGC CTTTTGCAAC AGATGTTCTG 
GGCGACACTG TGATTCACTT AGATCAGGCA TTAGCCAGAA TGAGAGACTA TACGCATATG 
AAGATGGAGG CTGAAAGTAG TCCAGACGTC AGGTGCACCT GCAGGATCAT TGAGGATGAC 
GATGGTGCTG CTGCCACAAC CACGATTAGT AATAATTTAG AAGAAACTCC TGTTATTGAA 
AATCATAGTT CACAGCAACC CGTGAGTGAA GTTGCTACCG TCCCGTGTCC TAGAATTGAT 
ACTCAGCAAC TGGACCGGCA AATTAAAGCA ATTATGAAAG AAGTCATTCC TTTTTTGAAG 
GAGCACATGG ATGAAGTATG TTCTTCTCAA CTTCTAACCT CAGTAAGACG CATGGTTTTG 
GTCCTTACTC AGCAAAACAA TGAGAGCAAA GAGTTTGTAA AGTTCTTTCA TAAGCAGCTT 
GGAAGTATAT TACAGGATTC ACTCGCAAAA TTTGCTGGCA GAAAACTGAA GGATTGTGGA 
GAAGATCTTC TTGTAGAGAT ATCTGAAGTG TTATTTAATG AATTGGCTTT CTTTAAGCTC 
ATGCAAGATT TGGATAATAA TAGTGTAACT GTTAAACAGA GATGCAAAAG GAAAATAGAA 
GCTGCTGGAG TGATACAGTC TTATGCAAAA GAGGCCAAAA GGATTCTTGA AGGAGATCAC 
AGTTCACCTG CTGGAGAAAT TGATGATGAA GACAAAGACA AGGATGAGAC GGAAACAGTT 
AAGCAGACTC AAACGTCTGA GGTTTATGAT GGTGACGGCC CCAAAAATGT GAGATCTGAT 
GTTTCTGATC AAGAGGATGA TGAAGAAAGT GAAGAATGTC CAGTGTCTAT TAATTTGTCC 
AAAGCTGAAA CTCAGGCTTT GACTAATTAC GGAAGTGGAG AAGATGAGAA TGAAGATGAA 
GAAATAGAAG AATTTGAAGA GGGACCTGTA GATGTCCAGA CGTCACTTCA GGCTAACACT 
GAAACTACAG AAGAAAATGA ACACGATGAT CAGGTTCCAC AGCATGATTT TGAAAAATCA 
TCAGAAAGCA AAAATGTCCC ATCAGAACAA GAACCCACTA CTAGTAAAGA CTTTCTTTTG 
ATGACAGGTG ACCAGAATAG CACTCCTGTG AAGCCCTGTT ACCTCAGTAT CGTGGAAAAT 
GAGCAACCTT TAAATAGCCC TGTCCAGAAG GACACTCTCA CTACCATCGA CTCATCCAGT 
CAGCCTAACC CTTTGCCTTT ACCTTTAACT GAAATTGAAA CCTTGGTGCC TAGAGTCAAA 
GAAGTGAAAT CTGCTCAGGA GACTCCTGAA AGTTCTTTGG CTGGAAGTCC TGATACTGAA 
TCTCCAGTGT TAGTGAATGA CTATGAAGCA GAATCTGGTA ACATAAGTCA AAAATCTGAT 
GAAGAAGACT TTGTGAAAGT TGAAGATTTA CCACTTAAAC TGACAATATA TTCAGAGGCA 
GATCTAAGGA AGAAAATGGC CGAAGAAGAA CAGAAAAACC ACTTATCTGG TGAAATATGT 
GCAATGCAGA CTGAAGAATT AGCTGGGAAT TCTCAGACAC TAAAAGAACC TGAAACAGTG 
GGAGCCCAAA GTACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025135245.1
CDS	392..6949
Translation	MATGGGPFEEGVNDQDLPNWSNESVDDRLNNMDWGSQQKKANRSSEKNKRKFGVESDKRVTNDISPESSPGVGRRRTKTSLTFPHSRYMTQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDATVSPQKREMIGSAQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIREYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLQKILARENEEEDVRTIDSAVGSGSVAESTSLNIDVRSEASDATEASFSLRIRPCVEDKLGNSASQAQVSDTDVTTSPKGKGDRPPQHDRELRPNRKCSRKRGFPSKARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSEKVAETTPGHHTVPGRQPAHSPLHQRVPIRETTGSLSGVSITSELNEELNDLIQHFHNQLRDSQPPAVPDNRRQAESLSLTREISQSRNPSVSEHLPDEKVQLFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSFVPSSASPQRSVDQRSTTTAPSAPVVLTPVVNGESNNLTPSVPYPAASLVSQNQNENEGHLNPTEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENTKEEETEESEYDSEHENSEPVTNIRNPQVAATWNEVNSNSNAQCVSNNRDGRAVNSNCEINNRSAANIRALNMPPLSADCRYNREGEQEMPVAQGHEEEEEEEEEAEDEALSGASLSSHSSSLIIETPEDAEFEHKIQKLMAAKLKLRHLQNLVAMVQDDDAANQGVTSVEDFFPAEDTKQNANNTRGNANKTQKDAGVNEKAREKFYEAKLQQQQRELKQLQEERKKLIEIQEKIQELQKACPNLQLSAASVGNCPTKTHMPAVTSSPTVNENEASTNKSGFEPHDSSVVDNEVWSDMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETASPVAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGIIRTDEEEEEEEQDASSNDNFHMYPPNSANHNSYNVKETKNRWKNNRPFSADGNYRPLAKTRQQNISMQRQENLRWVSELSYVEEKEQWQEQINQLKKQLDFSVNICQTLMQDQQTLSCLLQTLLTSPYSVMPSSVASPQVHLIMHQLNQCYTQLTWQQNNVQRLKQMLSELMRQQNQHPEKPASKEGGSGTSHPPSPSLFCPFNFPTQPVNLFNLPGFTNFSSFAPSMNFSPLFPSNFGDFSQNISTPTEQQQPLAQNPSGKTEYMAFPKPFESSSSIGAEKQRNQKQPEEEVENSRTPWFYDQEGEVEKPFLKTGFAVSVEKTTNSNRKNQLDTSRRRRQFDEVSLESFNSMLDPVDPTTVTKTFKSRKASAQASLASKDKTPKSKSKKRHSTQLKSRVKNIGYESASVSSTCEPCKSRNRHSAQTEEPVQAKVLSRKNHEQLEKVIRCSRSTEISSAHARRILQQSNRNACNEAPETGSDFSMFEALRDTIYSEVATLISQNESRPHFLIELFHELQLLNTDYLRQRALYALQDIVSRHISENHEKEGENIKSVNSGTWIASNSELTPSESLATTDDETFEKNFERETHKISEQNDADNASVSSTCEPFATDVLGDTVIHLDQALARMRDYTHMKMEAESSPDVRCTCRIIEDDDGAAATTTISNNLEETPVIENHSSQQPVSEVATVPCPRIDTQQLDRQIKAIMKEVIPFLKEHMDEVCSSQLLTSVRRMVLVLTQQNNESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSVTVKQRCKRKIEAAGVIQSYAKEAKRILEGDHSSPAGEIDDEDKDKDETETVKQTQTSEVYDGDGPKNVRSDVSDQEDDEESEECPVSINLSKAETQALTNYGSGEDENEDEEIEEFEEGPVDVQTSLQANTETTEENEHDDQVPQHDFEKSSESKNVPSEQEPTTSKDFLLMTGDQNSTPVKPCYLSIVENEQPLNSPVQKDTLTTIDSSSQPNPLPLPLTEIETLVPRVKEVKSAQETPESSLAGSPDTESPVLVNDYEAESGNISQKSDEEDFVKVEDLPLKLTIYSEADLRKKMAEEEQKNHLSGEICAMQTEELAGNSQTLKEPETVGAQST

Target ORF information:

RefSeq Version	XM_025279460.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis pericentriolar material 1 (PCM1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025279460.1

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
AGCAATGAGA GTGTTGATGA CCGACTCAAC AATATGGATT GGGGCAGCCA GCAGAAGAAG 
GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA 
ACTAACGATA TTTCTCCGGA GTCATCACCG GGAGTTGGAA GACGAAGAAC AAAGACTTCA 
CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TCCCCGAGCA GGCAGAACTA 
GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCTCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCCAC TGTAAGTCCT 
CAAAAGAGAG AAATGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAGATTCGTG AGTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGACCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTCACT 
CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT CCAGAAGATT 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCGGT CTGAGGCTTC AGATGCCACG 
GAGGCATCTT TTAGTCTGAG AATTCGGCCG TGCGTTGAGG ACAAACTAGG GAATTCAGCT 
TCACAAGCAC AGGTTTCAGA CACTGACGTT ACTACAAGTC CAAAAGGGAA AGGTGACAGA 
CCTCCTCAGC ATGACAGGGA GCTGAGGCCT AATAGGAAAT GTAGCCGAAA ACGTGGATTT 
CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATGGAAG AGATAGAAAA TTTGAAGAAA 
CAACATGATT TATTAAAAAG GATGTTACAG CAGCAGGAGC AGCTGAGAGC TCTACAAGGA 
CGACAAGCTG CTCTGCTTGC CCTGCAGCAT AAAGCTGAGC AGGCTATAGC TGTGATGGAT 
GATTCTGAAA AGGTTGCGGA GACAACTCCT GGCCATCATA CTGTACCAGG CAGACAGCCG 
GCTCATTCAC CTTTGCATCA GAGAGTACCC ATAAGAGAAA CTACAGGTAG CCTATCTGGA 
GTCAGTATCA CCTCTGAACT AAACGAAGAA TTGAATGATT TAATTCAGCA TTTTCATAAT 
CAGCTTCGTG ATTCTCAGCC TCCAGCTGTT CCAGACAATA GAAGACAAGC AGAAAGTCTT 
TCATTAACTA GGGAGATTTC TCAGAGCAGA AATCCTTCAG TTTCAGAGCA TTTACCTGAT 
GAGAAAGTCC AACTTTTTAG CAAGATGAGA GTGTTACAAG AAAAGAAACA AAAAATGGAC 
AAACTTCTTG GAGAACTTCA TACACTTCGA GATCAGCATT TGAACAATTC ATCATTTGTG 
CCTTCTTCAG CCTCTCCACA AAGGAGTGTG GATCAGAGAA GTACAACCAC AGCTCCTTCT 
GCTCCTGTAG TCTTAACACC AGTGGTCAAT GGAGAATCCA ATAACCTCAC GCCATCTGTT 
CCTTATCCTG CTGCTTCTCT AGTTTCTCAG AATCAGAATG AAAATGAAGG CCACCTAAAT 
CCAACTGAAA AACTCCAGAA GTTAAATGAA GTTCGAAAGA GGTTGAATGA ACTAAGAGAA 
TTAGTTCATT ATTATGAGCA AACATCAGAT ATGATGACAG ATGCTGTGAA TGAAAACACA 
AAAGAAGAAG AAACTGAAGA ATCAGAATAT GATTCGGAGC ATGAAAACTC GGAACCTGTT 
ACTAACATTC GAAATCCACA AGTGGCTGCC ACCTGGAACG AAGTAAATAG TAATTCTAAT 
GCACAGTGTG TTTCCAACAA TAGAGATGGG CGAGCAGTTA ATTCTAACTG TGAAATTAAC 
AACAGGTCTG CTGCCAACAT AAGGGCTCTA AACATGCCTC CTCTTTCAGC AGATTGTCGA 
TACAATAGAG AAGGAGAACA GGAGATGCCT GTTGCACAAG GTCATGAGGA GGAGGAGGAG 
GAGGAGGAGG AAGCAGAAGA TGAGGCCCTC AGTGGAGCTT CACTGTCTAG TCACAGCAGC 
AGCCTGATCA TTGAGACTCC TGAAGATGCT GAATTTGAAC ATAAGATCCA GAAGCTTATG 
GCTGCAAAAC TGAAACTTAG ACACTTACAG AACCTTGTAG CCATGGTGCA GGACGATGAT 
GCAGCCAATC AAGGAGTTAC TTCTGTGGAG GATTTCTTCC CAGCAGAAGA CACCAAACAA 
AATGCAAATA ACACTAGAGG AAATGCCAAT AAAACACAGA AAGATGCTGG AGTAAATGAA 
AAGGCAAGGG AGAAATTTTA TGAGGCTAAA CTACAGCAGC AACAGAGAGA ACTCAAACAA 
TTGCAGGAAG AAAGAAAGAA ACTGATTGAG ATTCAAGAGA AAATTCAAGA ATTGCAAAAG 
GCATGTCCTA ACCTACAGCT GTCAGCTGCT AGTGTGGGTA ATTGTCCCAC AAAAACACAT 
ATGCCAGCTG TTACTTCATC CCCAACCGTT AATGAAAATG AAGCCAGTAC AAACAAATCT 
GGTTTTGAGC CTCATGATTC TTCAGTGGTA GATAATGAGG TATGGTCAGA TATGCGAAGA 
CATGAAATGT TAAGGGAGGA GCTACGACAG AGAAGAAAGC AGCTGGAAGC TCTGATGGCT 
GAACATCAGA GGAGGCAAGG TCTGGCTGAA ACTGCATCTC CAGTGGCTGT TTCACTGAGA 
AGTGATGGAT CTGAGAACCT GTGTACACCT CAGCAAAGCA GAACAGAAAA AACAATGGCA 
ACTTGGGGAG GTTCTACCCA GTGTGCCCTA GATGAAGAAG GAGATGAAGA TGGTTACCTT 
TCTGAAGGAA TTATTCGGAC AGACGAAGAG GAGGAAGAGG AGGAGCAAGA TGCCAGTTCC 
AATGATAACT TTCATATGTA TCCTCCAAAC AGTGCAAATC ATAATTCTTA CAATGTAAAG 
GAAACTAAAA ATAGGTGGAA GAACAATCGC CCTTTTTCAG CAGATGGAAA TTATCGTCCT 
TTAGCCAAGA CAAGACAGCA GAATATCAGC ATGCAGCGTC AGGAAAACCT TCGCTGGGTG 
TCCGAACTCT CTTATGTAGA AGAGAAAGAA CAATGGCAAG AACAAATTAA TCAGCTAAAG 
AAACAACTTG ATTTCAGTGT CAATATTTGT CAGACTTTAA TGCAAGACCA GCAGACTCTG 
TCTTGTCTGC TACAAACTCT TCTCACGAGT CCTTATAGTG TTATGCCCAG CAGTGTTGCA 
TCCCCGCAAG TGCACCTTAT AATGCACCAG TTGAATCAGT GCTATACCCA GCTAACGTGG 
CAGCAGAACA ATGTCCAGAG GTTGAAACAA ATGCTCAGTG AACTCATGCG CCAACAAAAT 
CAGCATCCAG AAAAACCTGC AAGCAAGGAA GGAGGCAGTG GTACATCACA CCCTCCTTCT 
CCCAGTTTAT TTTGTCCTTT CAACTTTCCA ACTCAGCCTG TAAATCTGTT TAACCTACCG 
GGGTTTACTA ATTTTTCGTC ATTTGCACCA AGTATGAATT TCAGCCCTTT ATTTCCTTCT 
AATTTTGGAG ATTTTTCTCA AAATATTTCT ACACCCACTG AACAGCAGCA ACCGTTAGCC 
CAGAATCCTT CAGGGAAAAC GGAATACATG GCTTTTCCAA AACCTTTTGA AAGTAGTTCT 
TCTATTGGAG CGGAAAAACA AAGGAATCAA AAACAGCCTG AAGAGGAGGT GGAGAACAGT 
AGGACACCAT GGTTTTATGA CCAAGAAGGT GAAGTAGAAA AGCCATTTCT CAAGACTGGA 
TTTGCAGTGT CTGTAGAGAA AACAACAAAT AGTAATCGCA AAAATCAACT AGATACCAGT 
AGGAGAAGAC GCCAGTTTGA TGAAGTGTCC TTAGAAAGCT TTAACAGTAT GCTTGACCCA 
GTAGATCCAA CAACAGTAAC TAAAACATTC AAGTCAAGAA AAGCATCTGC ACAGGCCAGC 
CTGGCATCTA AAGATAAAAC TCCCAAATCA AAGAGTAAGA AGAGGCATTC TACTCAGCTG 
AAAAGCAGAG TGAAAAATAT CGGGTATGAA AGTGCCAGTG TGTCTAGCAC ATGTGAACCT 
TGCAAAAGTA GAAACAGACA TTCAGCCCAA ACTGAAGAGC CTGTTCAAGC AAAAGTACTC 
AGCAGAAAGA ATCATGAGCA GCTGGAAAAA GTCATAAGAT GTAGTAGGTC TACAGAAATA 
TCTTCAGCAC ATGCTAGGAG AATACTACAG CAGTCTAACA GAAATGCATG CAATGAAGCG 
CCAGAAACCG GGAGTGATTT TTCCATGTTC GAAGCTTTGC GGGATACCAT TTATTCTGAA 
GTAGCTACGT TGATTTCTCA AAATGAATCT CGTCCACATT TTCTTATTGA ACTCTTCCAT 
GAGCTTCAGC TACTTAATAC AGACTATTTG AGACAGAGGG CCTTATATGC ATTGCAGGAC 
ATAGTATCCA GGCATATTTC TGAGAACCAT GAAAAAGAAG GAGAAAATAT AAAGTCCGTG 
AATTCTGGTA CTTGGATAGC ATCCAACTCA GAACTTACTC CCAGTGAAAG CCTTGCTACT 
ACTGATGATG AAACTTTTGA GAAGAACTTT GAGAGAGAAA CACATAAAAT AAGTGAGCAA 
AATGATGCTG ATAATGCTAG TGTATCTTCA ACTTGTGAGC CTTTTGCAAC AGATGTTCTG 
GGCGACACTG TGATTCACTT AGATCAGGCA TTAGCCAGAA TGAGAGACTA TACGCATATG 
AAGATGGAGG CTGAAAGTAG TCCAGACGTC AGGTGCACCT GCAGGATCAT TGAGGATGAC 
GATGGTGCTG CTGCCACAAC CACGATTAGT AATAATTTAG AAGAAACTCC TGTTATTGAA 
AATCATAGTT CACAGCAACC CGTGAGTGAA GTTGCTACCG TCCCGTGTCC TAGAATTGAT 
ACTCAGCAAC TGGACCGGCA AATTAAAGCA ATTATGAAAG AAGTCATTCC TTTTTTGAAG 
GAGCACATGG ATGAAGTATG TTCTTCTCAA CTTCTAACCT CAGTAAGACG CATGGTTTTG 
GTCCTTACTC AGCAAAACAA TGAGAGCAAA GAGTTTGTAA AGTTCTTTCA TAAGCAGCTT 
GGAAGTATAT TACAGGATTC ACTCGCAAAA TTTGCTGGCA GAAAACTGAA GGATTGTGGA 
GAAGATCTTC TTGTAGAGAT ATCTGAAGTG TTATTTAATG AATTGGCTTT CTTTAAGCTC 
ATGCAAGATT TGGATAATAA TAGTGTAACT GTTAAACAGA GATGCAAAAG GAAAATAGAA 
GCTGCTGGAG TGATACAGTC TTATGCAAAA GAGGCCAAAA GGATTCTTGA AGGAGATCAC 
AGTTCACCTG CTGGAGAAAT TGATGATGAA GACAAAGACA AGGATGAGAC GGAAACAGTT 
AAGCAGACTC AAACGTCTGA GGTTTATGAT GGTGACGGCC CCAAAAATGT GAGATCTGAT 
GTTTCTGATC AAGAGGATGA TGAAGAAAGT GAAGAATGTC CAGTGTCTAT TAATTTGTCC 
AAAGCTGAAA CTCAGGCTTT GACTAATTAC GGAAGTGGAG AAGATGAGAA TGAAGATGAA 
GAAATAGAAG AATTTGAAGA GGGACCTGTA GATGTCCAGA CGTCACTTCA GGCTAACACT 
GAAACTACAG AAGAAAATGA ACACGATGAT CAGGTTCCAC AGCATGATTT TGAAAAATCA 
TCAGAAAGCA AAAATGTCCC ATCAGAACAA GAACCCACTA CTAGTAAAGA CTTTCTTTTG 
ATGACAGGTG ACCAGAATAG CACTCCTGTG AAGCCCTGTT ACCTCAGTAT CGTGGAAAAT 
GAGCAACCTT TAAATAGCCC TGTCCAGAAG GACACTCTCA CTACCATCGA CTCATCCAGT 
CAGCCTAACC CTTTGCCTTT ACCTTTAACT GAAATTGAAA CCTTGGTGCC TAGAGTCAAA 
GAAGTGAAAT CTGCTCAGGA GACTCCTGAA AGTTCTTTGG CTGGAAGTCC TGATACTGAA 
TCTCCAGTGT TAGTGAATGA CTATGAAGCA GAATCTGGTA ACATAAGTCA AAAATCTGAT 
GAAGAAGACT TTGTGAAAGT TGAAGATTTA CCACTTAAAC TGACAATATA TTCAGAGGCA 
GATCTAAGGA AGAAAATGGC CGAAGAAGAA CAGAAAAACC ACTTATCTGG TGAAATATGT 
GCAATGCAGA CTGAAGAATT AGCTGGGAAT TCTCAGACAC TAAAAGAACC TGAAACAGTG 
GGAGCCCAAA GTACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( XM_025279466.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025135251.1 Bubalus bubalis pericentriolar material 1 (PCM1), transcript variant X10, mRNA.

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CloneID	OBu42298
Clone ID Related Accession (Same CDS sequence)	XM_025279466.1
Accession Version	XM_025279466.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6543bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	pericentriolar material 1 protein isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037545.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG GAGGAGGTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
AGCAATGAGA GTGTTGATGA CCGACTCAAC AATATGGATT GGGGCAGCCA GCAGAAGAAG 
GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA 
ACTAACGATA TTTCTCCGGA GTCATCACCG GGAGTTGGAA GACGAAGAAC AAAGACTTCA 
CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TCCCCGAGCA GGCAGAACTA 
GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCTCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCCAC TGTAAGTCCT 
CAAAAGAGAG AAATGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAGATTCGTG AGTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGACCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTCACT 
CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT CCAGAAGATT 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCGGT CTGAGGCTTC AGATGCCACG 
GAGGCATCTT TTAGTCTGAG AATTCGGCCG TGCGTTGAGG ACAAACTAGG GAATTCAGCT 
TCACAAGCAC AGGTTTCAGA CACTGACGTT ACTACAAGTC CAAAAGGGAA AGGTGACAGA 
CCTCCTCAGC ATGACAGGGA GCTGAGGCCT AATAGGAAAT GTAGCCGAAA ACGTGGATTT 
CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATGGAAG AGATAGAAAA TTTGAAGAAA 
CAACATGATT TATTAAAAAG GATGTTACAG CAGCAGGAGC AGCTGAGAGC TCTACAAGGA 
CGACAAGCTG CTCTGCTTGC CCTGCAGCAT AAAGCTGAGC AGGCTATAGC TGTGATGGAT 
GATTCTGAAA AGGTTGCGGA GACAACTCCT GGCCATCATA CTGTACCAGG CAGACAGCCG 
GCTCATTCAC CTTTGCATCA GAGAGTACCC ATAAGAGAAA CTACAGGTAG CCTATCTGGA 
GTCAGTATCA CCTCTGAACT AAACGAAGAA TTGAATGATT TAATTCAGCA TTTTCATAAT 
CAGCTTCGTG ATTCTCAGCC TCCAGCTGTT CCAGACAATA GAAGACAAGC AGAAAGTCTT 
TCATTAACTA GGGAGATTTC TCAGAGCAGA AATCCTTCAG TTTCAGAGCA TTTACCTGAT 
GAGAAAGTCC AACTTTTTAG CAAGATGAGA GTGTTACAAG AAAAGAAACA AAAAATGGAC 
AAACTTCTTG GAGAACTTCA TACACTTCGA GATCAGCATT TGAACAATTC ATCATTTGTG 
CCTTCTTCAG CCTCTCCACA AAGGAGTGTG GATCAGAGAA GTACAACCAC AGCTCCTTCT 
GCTCCTGTAG TCTTAACACC AGTGGTCAAT GGAGAATCCA ATAACCTCAC GCCATCTGTT 
CCTTATCCTG CTGCTTCTCT AGTTTCTCAG AATCAGAATG AAAATGAAGG CCACCTAAAT 
CCAACTGAAA AACTCCAGAA GTTAAATGAA GTTCGAAAGA GGTTGAATGA ACTAAGAGAA 
TTAGTTCATT ATTATGAGCA AACATCAGAT ATGATGACAG ATGCTGTGAA TGAAAACACA 
AAAGAAGAAG AAACTGAAGA ATCAGAATAT GATTCGGAGC ATGAAAACTC GGAACCTGTT 
ACTAACATTC GAAATCCACA AGTGGCTGCC ACCTGGAACG AAGTAAATAG TAATTCTAAT 
GCACAGTGTG TTTCCAACAA TAGAGATGGG CGAGCAGTTA ATTCTAACTG TGAAATTAAC 
AACAGGTCTG CTGCCAACAT AAGGGCTCTA AACATGCCTC CTCTTTCAGC AGATTGTCGA 
TACAATAGAG AAGGAGAACA GGAGATGCCT GTTGCACAAG GTCATGAGGA GGAGGAGGAG 
GAGGAGGAGG AAGCAGAAGA TGAGGCCCTC AGTGGAGCTT CACTGTCTAG TCACAGCAGC 
AGCCTGATCA TTGAGACTCC TGAAGATGCT GAATTTGAAC ATAAGATCCA GAAGCTTATG 
GCTGCAAAAC TGAAACTTAG ACACTTACAG AACCTTGTAG CCATGGTGCA GGACGATGAT 
GCAGCCAATC AAGGAGTTAC TTCTGTGGAG GATTTCTTCC CAGCAGAAGA CACCAAACAA 
AATGCAAATA ACACTAGAGG AAATGCCAAT AAAACACAGA AAGATGCTGG AGTAAATGAA 
AAGGCAAGGG AGAAATTTTA TGAGGCTAAA CTACAGCAGC AACAGAGAGA ACTCAAACAA 
TTGCAGGAAG AAAGAAAGAA ACTGATTGAG ATTCAAGAGA AAATTCAAGA ATTGCAAAAG 
GCATGTCCTA ACCTACAGCT GTCAGCTGCT AGTGTGGGTA ATTGTCCCAC AAAAACACAT 
ATGCCAGCTG TTACTTCATC CCCAACCGTT AATGAAAATG AAGCCAGTAC AAACAAATCT 
GGTTTTGAGC CTCATGATTC TTCAGTGGTA GATAATGAGG TATGGTCAGA TATGCGAAGA 
CATGAAATGT TAAGGGAGGA GCTACGACAG AGAAGAAAGC AGCTGGAAGC TCTGATGGCT 
GAACATCAGA GGAGGCAAGG TCTGGCTGAA ACTGCATCTC CAGTGGCTGT TTCACTGAGA 
AGTGATGGAT CTGAGAACCT GTGTACACCT CAGCAAAGCA GAACAGAAAA AACAATGGCA 
ACTTGGGGAG GTTCTACCCA GTGTGCCCTA GATGAAGAAG GAGATGAAGA TGGTTACCTT 
TCTGAAGGAA TTATTCGGAC AGACGAAGAG GAGGAAGAGG AGGAGCAAGA TGCCAGTTCC 
AATGATAACT TTCATATGTA TCCTCCAAAC AGTGCAAATC ATAATTCTTA CAATGTAAAG 
GAAACTAAAA ATAGGTGGAA GAACAATCGC CCTTTTTCAG CAGATGGAAA TTATCGTCCT 
TTAGCCAAGA CAAGACAGCA GAATATCAGC ATGCAGCGTC AGGAAAACCT TCGCTGGGTG 
TCCGAACTCT CTTATGTAGA AGAGAAAGAA CAATGGCAAG AACAAATTAA TCAGCTAAAG 
AAACAACTTG ATTTCAGTGT CAATATTTGT CAGACTTTAA TGCAAGACCA GCAGACTCTG 
TCTTGTCTGC TACAAACTCT TCTCACGAGT CCTTATAGTG TTATGCCCAG CAGTGTTGCA 
TCCCCGCAAG TGCACCTTAT AATGCACCAG TTGAATCAGT GCTATACCCA GCTAACGTGG 
CAGCAGAACA ATGTCCAGAG GTTGAAACAA ATGCTCAGTG AACTCATGCG CCAACAAAAT 
CAGCATCCAG AAAAACCTGC AAGCAAGGAA GGAGGCAGTG GTACATCACA CCCTCCTTCT 
CCCAGTTTAT TTTGTCCTTT CAACTTTCCA ACTCAGCCTG TAAATCTGTT TAACCTACCG 
GGGTTTACTA ATTTTTCGTC ATTTGCACCA AGTATGAATT TCAGCCCTTT ATTTCCTTCT 
AATTTTGGAG ATTTTTCTCA AAATATTTCT ACACCCACTG AACAGCAGCA ACCGTTAGCC 
CAGAATCCTT CAGGGAAAAC GGAATACATG GCTTTTCCAA AACCTTTTGA AAGTAGTTCT 
TCTATTGGAG CGGAAAAACA AAGGAATCAA AAACAGCCTG AAGAGGAGGT GGAGAACAGT 
AGGACACCAT GGTTTTATGA CCAAGAAGGT GAAGTAGAAA AGCCATTTCT CAAGACTGGA 
TTTGCAGTGT CTGTAGAGAA AACAACAAAT AGTAATCGCA AAAATCAACT AGATACCAGT 
AGGAGAAGAC GCCAGTTTGA TGAAGTGTCC TTAGAAAGCT TTAACAGTAT GCTTGACCCA 
GTAGATCCAA CAACAGTAAC TAAAACATTC AAGTCAAGAA AAGCATCTGC ACAGGCCAGC 
CTGGCATCTA AAGATAAAAC TCCCAAATCA AAGAGTAAGA AGAGGCATTC TACTCAGCTG 
AAAAGCAGAG TGAAAAATAT CGGGTATGAA AGTGCCAGTG TGTCTAGCAC ATGTGAACCT 
TGCAAAAGTA GAAACAGACA TTCAGCCCAA ACTGAAGAGC CTGTTCAAGC AAAAGTACTC 
AGCAGAAAGA ATCATGAGCA GCTGGAAAAA GTCATAAGAT GTAGTAGGTC TACAGAAATA 
TCTTCAGCAC ATGCTAGGAG AATACTACAG CAGTCTAACA GAAATGCATG CAATGAAGCG 
CCAGAAACCG GGAGTGATTT TTCCATGTTC GAAGCTTTGC GGGATACCAT TTATTCTGAA 
GTAGCTACGT TGATTTCTCA AAATGAATCT CGTCCACATT TTCTTATTGA ACTCTTCCAT 
GAGCTTCAGC TACTTAATAC AGACTATTTG AGACAGAGGG CCTTATATGC ATTGCAGGAC 
ATAGTATCCA GGCATATTTC TGAGAACCAT GAAAAAGAAG GAGAAAATAT AAAGTCCGTG 
AATTCTGGTA CTTGGATAGC ATCCAACTCA GAACTTACTC CCAGTGAAAG CCTTGCTACT 
ACTGATGATG AAACTTTTGA GAAGAACTTT GAGAGAGAAA CACATAAAAT AAGTGAGCAA 
AATGATGCTG ATAATGCTAG TGTATCTTCA ACTTGTGAGC CTTTTGCAAC AGATGTTCTG 
GGCGACACTG TGATTCACTT AGATCAGGCA TTAGCCAGAA TGAGAGACTA TACGCATATG 
AAGATGGAGG CTGAAAGTAG TCCAGACGTC AGGTGCACCT GCAGGATCAT TGAGGATGAC 
GATGGTGCTG CTGCCACAAC CACGATTAGT AATAATTTAG AAGAAACTCC TGTTATTGAA 
AATCATAGTT CACAGCAACC CGTGAGTGAA GTTGCTACCG TCCCGTGTCC TAGAATTGAT 
ACTCAGCAAC TGGACCGGCA AATTAAAGCA ATTATGAAAG AAGTCATTCC TTTTTTGAAG 
GAGCACATGG ATGAAGTATG TTCTTCTCAA CTTCTAACCT CAGTAAGACG CATGGTTTTG 
GTCCTTACTC AGCAAAACAA TGAGAGCAAA GAGTTTGTAA AGTTCTTTCA TAAGCAGCTT 
GGAAGTATAT TACAGGATTC ACTCGCAAAA TTTGCTGGCA GAAAACTGAA GGATTGTGGA 
GAAGATCTTC TTGTAGAGAT ATCTGAAGTG TTATTTAATG AATTGGCTTT CTTTAAGCTC 
ATGCAAGATT TGGATAATAA TAGTGTAACT GTTAAACAGA GATGCAAAAG GAAAATAGAA 
GCTGCTGGAG TGATACAGTC TTATGCAAAA GAGGCCAAAA GGATTCTTGA AGGAGATCAC 
AGTTCACCTG CTGGAGAAAT TGATGATGAA GACAAAGACA AGGATGAGAC GGAAACAGTT 
AAGCAGACTC AAACGTCTGA GGTTTATGAT GGTGACGGCC CCAAAAATGT GAGATCTGAT 
GTTTCTGATC AAGAGGATGA TGAAGAAAGT GAAGAATGTC CAGTGTCTAT TAATTTGTCC 
AAAGCTGAAA CTCAGGCTTT GACTAATTAC GGAAGTGGAG AAGATGAGAA TGAAGATGAA 
GAAATAGAAG AATTTGAAGA GGGACCTGTA GATGTCCAGA CGTCACTTCA GGCTAACACT 
GAAACTACAG AAGAAAATGA ACACGATGAT CAGGTTCCAC AGCATGATTT TGAAAAATCA 
TCAGAAAGCA AAAATGTCCC ATCAGAACAA GAACCCACTA CTAGTAAAGA CTTTCTTTTG 
ATGACAGGTG ACCAGAATAG CACTCCTGTG AAGCCCTGTT ACCTCAGTAT CGTGGAAAAT 
GAGCAACCTT TAAATAGCCC TGTCCAGAAG GACACTCTCA CTACCATCGA CTCATCCAGT 
CAGCCTAACC CTTTGCCTTT ACCTTTAACT GAAATTGAAA CCTTGGTGCC TAGAGTCAAA 
GAAGTGAAAT CTGCTCAGGA GACTCCTGAA AGTTCTTTGG CTGGAAGTCC TGATACTGAA 
TCTCCAGTGT TAGTGAATGA CTATGAAGCA GAATCTGGTA ACATAAGTCA AAAATCTGAT 
GAAGAAGACT TTGTGAAAGT TGAAGATTTA CCACTTAAAC TGACAATATA TTCAGAGGCA 
GATCTAAGGA AGAAAATGGC CGAAGAAGAA CAGAAAAACC ACTTATCTGG TGAAATATGT 
GCAATGCAGA CTGAAGAATT AGCTGGGAAT TCTCAGACAC TAAAAGAACC TGAAATAGGG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025135251.1
CDS	392..6934
Translation	MATGGGPFEEGVNDQDLPNWSNESVDDRLNNMDWGSQQKKANRSSEKNKRKFGVESDKRVTNDISPESSPGVGRRRTKTSLTFPHSRYMTQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDATVSPQKREMIGSAQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIREYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLQKILARENEEEDVRTIDSAVGSGSVAESTSLNIDVRSEASDATEASFSLRIRPCVEDKLGNSASQAQVSDTDVTTSPKGKGDRPPQHDRELRPNRKCSRKRGFPSKARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSEKVAETTPGHHTVPGRQPAHSPLHQRVPIRETTGSLSGVSITSELNEELNDLIQHFHNQLRDSQPPAVPDNRRQAESLSLTREISQSRNPSVSEHLPDEKVQLFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSFVPSSASPQRSVDQRSTTTAPSAPVVLTPVVNGESNNLTPSVPYPAASLVSQNQNENEGHLNPTEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENTKEEETEESEYDSEHENSEPVTNIRNPQVAATWNEVNSNSNAQCVSNNRDGRAVNSNCEINNRSAANIRALNMPPLSADCRYNREGEQEMPVAQGHEEEEEEEEEAEDEALSGASLSSHSSSLIIETPEDAEFEHKIQKLMAAKLKLRHLQNLVAMVQDDDAANQGVTSVEDFFPAEDTKQNANNTRGNANKTQKDAGVNEKAREKFYEAKLQQQQRELKQLQEERKKLIEIQEKIQELQKACPNLQLSAASVGNCPTKTHMPAVTSSPTVNENEASTNKSGFEPHDSSVVDNEVWSDMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETASPVAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGIIRTDEEEEEEEQDASSNDNFHMYPPNSANHNSYNVKETKNRWKNNRPFSADGNYRPLAKTRQQNISMQRQENLRWVSELSYVEEKEQWQEQINQLKKQLDFSVNICQTLMQDQQTLSCLLQTLLTSPYSVMPSSVASPQVHLIMHQLNQCYTQLTWQQNNVQRLKQMLSELMRQQNQHPEKPASKEGGSGTSHPPSPSLFCPFNFPTQPVNLFNLPGFTNFSSFAPSMNFSPLFPSNFGDFSQNISTPTEQQQPLAQNPSGKTEYMAFPKPFESSSSIGAEKQRNQKQPEEEVENSRTPWFYDQEGEVEKPFLKTGFAVSVEKTTNSNRKNQLDTSRRRRQFDEVSLESFNSMLDPVDPTTVTKTFKSRKASAQASLASKDKTPKSKSKKRHSTQLKSRVKNIGYESASVSSTCEPCKSRNRHSAQTEEPVQAKVLSRKNHEQLEKVIRCSRSTEISSAHARRILQQSNRNACNEAPETGSDFSMFEALRDTIYSEVATLISQNESRPHFLIELFHELQLLNTDYLRQRALYALQDIVSRHISENHEKEGENIKSVNSGTWIASNSELTPSESLATTDDETFEKNFERETHKISEQNDADNASVSSTCEPFATDVLGDTVIHLDQALARMRDYTHMKMEAESSPDVRCTCRIIEDDDGAAATTTISNNLEETPVIENHSSQQPVSEVATVPCPRIDTQQLDRQIKAIMKEVIPFLKEHMDEVCSSQLLTSVRRMVLVLTQQNNESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSVTVKQRCKRKIEAAGVIQSYAKEAKRILEGDHSSPAGEIDDEDKDKDETETVKQTQTSEVYDGDGPKNVRSDVSDQEDDEESEECPVSINLSKAETQALTNYGSGEDENEDEEIEEFEEGPVDVQTSLQANTETTEENEHDDQVPQHDFEKSSESKNVPSEQEPTTSKDFLLMTGDQNSTPVKPCYLSIVENEQPLNSPVQKDTLTTIDSSSQPNPLPLPLTEIETLVPRVKEVKSAQETPESSLAGSPDTESPVLVNDYEAESGNISQKSDEEDFVKVEDLPLKLTIYSEADLRKKMAEEEQKNHLSGEICAMQTEELAGNSQTLKEPEIG

Target ORF information:

RefSeq Version	XM_025279466.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis pericentriolar material 1 (PCM1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025279466.1

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
AGCAATGAGA GTGTTGATGA CCGACTCAAC AATATGGATT GGGGCAGCCA GCAGAAGAAG 
GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA 
ACTAACGATA TTTCTCCGGA GTCATCACCG GGAGTTGGAA GACGAAGAAC AAAGACTTCA 
CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TCCCCGAGCA GGCAGAACTA 
GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCTCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCCAC TGTAAGTCCT 
CAAAAGAGAG AAATGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAGATTCGTG AGTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGACCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTCACT 
CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT CCAGAAGATT 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCGGT CTGAGGCTTC AGATGCCACG 
GAGGCATCTT TTAGTCTGAG AATTCGGCCG TGCGTTGAGG ACAAACTAGG GAATTCAGCT 
TCACAAGCAC AGGTTTCAGA CACTGACGTT ACTACAAGTC CAAAAGGGAA AGGTGACAGA 
CCTCCTCAGC ATGACAGGGA GCTGAGGCCT AATAGGAAAT GTAGCCGAAA ACGTGGATTT 
CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATGGAAG AGATAGAAAA TTTGAAGAAA 
CAACATGATT TATTAAAAAG GATGTTACAG CAGCAGGAGC AGCTGAGAGC TCTACAAGGA 
CGACAAGCTG CTCTGCTTGC CCTGCAGCAT AAAGCTGAGC AGGCTATAGC TGTGATGGAT 
GATTCTGAAA AGGTTGCGGA GACAACTCCT GGCCATCATA CTGTACCAGG CAGACAGCCG 
GCTCATTCAC CTTTGCATCA GAGAGTACCC ATAAGAGAAA CTACAGGTAG CCTATCTGGA 
GTCAGTATCA CCTCTGAACT AAACGAAGAA TTGAATGATT TAATTCAGCA TTTTCATAAT 
CAGCTTCGTG ATTCTCAGCC TCCAGCTGTT CCAGACAATA GAAGACAAGC AGAAAGTCTT 
TCATTAACTA GGGAGATTTC TCAGAGCAGA AATCCTTCAG TTTCAGAGCA TTTACCTGAT 
GAGAAAGTCC AACTTTTTAG CAAGATGAGA GTGTTACAAG AAAAGAAACA AAAAATGGAC 
AAACTTCTTG GAGAACTTCA TACACTTCGA GATCAGCATT TGAACAATTC ATCATTTGTG 
CCTTCTTCAG CCTCTCCACA AAGGAGTGTG GATCAGAGAA GTACAACCAC AGCTCCTTCT 
GCTCCTGTAG TCTTAACACC AGTGGTCAAT GGAGAATCCA ATAACCTCAC GCCATCTGTT 
CCTTATCCTG CTGCTTCTCT AGTTTCTCAG AATCAGAATG AAAATGAAGG CCACCTAAAT 
CCAACTGAAA AACTCCAGAA GTTAAATGAA GTTCGAAAGA GGTTGAATGA ACTAAGAGAA 
TTAGTTCATT ATTATGAGCA AACATCAGAT ATGATGACAG ATGCTGTGAA TGAAAACACA 
AAAGAAGAAG AAACTGAAGA ATCAGAATAT GATTCGGAGC ATGAAAACTC GGAACCTGTT 
ACTAACATTC GAAATCCACA AGTGGCTGCC ACCTGGAACG AAGTAAATAG TAATTCTAAT 
GCACAGTGTG TTTCCAACAA TAGAGATGGG CGAGCAGTTA ATTCTAACTG TGAAATTAAC 
AACAGGTCTG CTGCCAACAT AAGGGCTCTA AACATGCCTC CTCTTTCAGC AGATTGTCGA 
TACAATAGAG AAGGAGAACA GGAGATGCCT GTTGCACAAG GTCATGAGGA GGAGGAGGAG 
GAGGAGGAGG AAGCAGAAGA TGAGGCCCTC AGTGGAGCTT CACTGTCTAG TCACAGCAGC 
AGCCTGATCA TTGAGACTCC TGAAGATGCT GAATTTGAAC ATAAGATCCA GAAGCTTATG 
GCTGCAAAAC TGAAACTTAG ACACTTACAG AACCTTGTAG CCATGGTGCA GGACGATGAT 
GCAGCCAATC AAGGAGTTAC TTCTGTGGAG GATTTCTTCC CAGCAGAAGA CACCAAACAA 
AATGCAAATA ACACTAGAGG AAATGCCAAT AAAACACAGA AAGATGCTGG AGTAAATGAA 
AAGGCAAGGG AGAAATTTTA TGAGGCTAAA CTACAGCAGC AACAGAGAGA ACTCAAACAA 
TTGCAGGAAG AAAGAAAGAA ACTGATTGAG ATTCAAGAGA AAATTCAAGA ATTGCAAAAG 
GCATGTCCTA ACCTACAGCT GTCAGCTGCT AGTGTGGGTA ATTGTCCCAC AAAAACACAT 
ATGCCAGCTG TTACTTCATC CCCAACCGTT AATGAAAATG AAGCCAGTAC AAACAAATCT 
GGTTTTGAGC CTCATGATTC TTCAGTGGTA GATAATGAGG TATGGTCAGA TATGCGAAGA 
CATGAAATGT TAAGGGAGGA GCTACGACAG AGAAGAAAGC AGCTGGAAGC TCTGATGGCT 
GAACATCAGA GGAGGCAAGG TCTGGCTGAA ACTGCATCTC CAGTGGCTGT TTCACTGAGA 
AGTGATGGAT CTGAGAACCT GTGTACACCT CAGCAAAGCA GAACAGAAAA AACAATGGCA 
ACTTGGGGAG GTTCTACCCA GTGTGCCCTA GATGAAGAAG GAGATGAAGA TGGTTACCTT 
TCTGAAGGAA TTATTCGGAC AGACGAAGAG GAGGAAGAGG AGGAGCAAGA TGCCAGTTCC 
AATGATAACT TTCATATGTA TCCTCCAAAC AGTGCAAATC ATAATTCTTA CAATGTAAAG 
GAAACTAAAA ATAGGTGGAA GAACAATCGC CCTTTTTCAG CAGATGGAAA TTATCGTCCT 
TTAGCCAAGA CAAGACAGCA GAATATCAGC ATGCAGCGTC AGGAAAACCT TCGCTGGGTG 
TCCGAACTCT CTTATGTAGA AGAGAAAGAA CAATGGCAAG AACAAATTAA TCAGCTAAAG 
AAACAACTTG ATTTCAGTGT CAATATTTGT CAGACTTTAA TGCAAGACCA GCAGACTCTG 
TCTTGTCTGC TACAAACTCT TCTCACGAGT CCTTATAGTG TTATGCCCAG CAGTGTTGCA 
TCCCCGCAAG TGCACCTTAT AATGCACCAG TTGAATCAGT GCTATACCCA GCTAACGTGG 
CAGCAGAACA ATGTCCAGAG GTTGAAACAA ATGCTCAGTG AACTCATGCG CCAACAAAAT 
CAGCATCCAG AAAAACCTGC AAGCAAGGAA GGAGGCAGTG GTACATCACA CCCTCCTTCT 
CCCAGTTTAT TTTGTCCTTT CAACTTTCCA ACTCAGCCTG TAAATCTGTT TAACCTACCG 
GGGTTTACTA ATTTTTCGTC ATTTGCACCA AGTATGAATT TCAGCCCTTT ATTTCCTTCT 
AATTTTGGAG ATTTTTCTCA AAATATTTCT ACACCCACTG AACAGCAGCA ACCGTTAGCC 
CAGAATCCTT CAGGGAAAAC GGAATACATG GCTTTTCCAA AACCTTTTGA AAGTAGTTCT 
TCTATTGGAG CGGAAAAACA AAGGAATCAA AAACAGCCTG AAGAGGAGGT GGAGAACAGT 
AGGACACCAT GGTTTTATGA CCAAGAAGGT GAAGTAGAAA AGCCATTTCT CAAGACTGGA 
TTTGCAGTGT CTGTAGAGAA AACAACAAAT AGTAATCGCA AAAATCAACT AGATACCAGT 
AGGAGAAGAC GCCAGTTTGA TGAAGTGTCC TTAGAAAGCT TTAACAGTAT GCTTGACCCA 
GTAGATCCAA CAACAGTAAC TAAAACATTC AAGTCAAGAA AAGCATCTGC ACAGGCCAGC 
CTGGCATCTA AAGATAAAAC TCCCAAATCA AAGAGTAAGA AGAGGCATTC TACTCAGCTG 
AAAAGCAGAG TGAAAAATAT CGGGTATGAA AGTGCCAGTG TGTCTAGCAC ATGTGAACCT 
TGCAAAAGTA GAAACAGACA TTCAGCCCAA ACTGAAGAGC CTGTTCAAGC AAAAGTACTC 
AGCAGAAAGA ATCATGAGCA GCTGGAAAAA GTCATAAGAT GTAGTAGGTC TACAGAAATA 
TCTTCAGCAC ATGCTAGGAG AATACTACAG CAGTCTAACA GAAATGCATG CAATGAAGCG 
CCAGAAACCG GGAGTGATTT TTCCATGTTC GAAGCTTTGC GGGATACCAT TTATTCTGAA 
GTAGCTACGT TGATTTCTCA AAATGAATCT CGTCCACATT TTCTTATTGA ACTCTTCCAT 
GAGCTTCAGC TACTTAATAC AGACTATTTG AGACAGAGGG CCTTATATGC ATTGCAGGAC 
ATAGTATCCA GGCATATTTC TGAGAACCAT GAAAAAGAAG GAGAAAATAT AAAGTCCGTG 
AATTCTGGTA CTTGGATAGC ATCCAACTCA GAACTTACTC CCAGTGAAAG CCTTGCTACT 
ACTGATGATG AAACTTTTGA GAAGAACTTT GAGAGAGAAA CACATAAAAT AAGTGAGCAA 
AATGATGCTG ATAATGCTAG TGTATCTTCA ACTTGTGAGC CTTTTGCAAC AGATGTTCTG 
GGCGACACTG TGATTCACTT AGATCAGGCA TTAGCCAGAA TGAGAGACTA TACGCATATG 
AAGATGGAGG CTGAAAGTAG TCCAGACGTC AGGTGCACCT GCAGGATCAT TGAGGATGAC 
GATGGTGCTG CTGCCACAAC CACGATTAGT AATAATTTAG AAGAAACTCC TGTTATTGAA 
AATCATAGTT CACAGCAACC CGTGAGTGAA GTTGCTACCG TCCCGTGTCC TAGAATTGAT 
ACTCAGCAAC TGGACCGGCA AATTAAAGCA ATTATGAAAG AAGTCATTCC TTTTTTGAAG 
GAGCACATGG ATGAAGTATG TTCTTCTCAA CTTCTAACCT CAGTAAGACG CATGGTTTTG 
GTCCTTACTC AGCAAAACAA TGAGAGCAAA GAGTTTGTAA AGTTCTTTCA TAAGCAGCTT 
GGAAGTATAT TACAGGATTC ACTCGCAAAA TTTGCTGGCA GAAAACTGAA GGATTGTGGA 
GAAGATCTTC TTGTAGAGAT ATCTGAAGTG TTATTTAATG AATTGGCTTT CTTTAAGCTC 
ATGCAAGATT TGGATAATAA TAGTGTAACT GTTAAACAGA GATGCAAAAG GAAAATAGAA 
GCTGCTGGAG TGATACAGTC TTATGCAAAA GAGGCCAAAA GGATTCTTGA AGGAGATCAC 
AGTTCACCTG CTGGAGAAAT TGATGATGAA GACAAAGACA AGGATGAGAC GGAAACAGTT 
AAGCAGACTC AAACGTCTGA GGTTTATGAT GGTGACGGCC CCAAAAATGT GAGATCTGAT 
GTTTCTGATC AAGAGGATGA TGAAGAAAGT GAAGAATGTC CAGTGTCTAT TAATTTGTCC 
AAAGCTGAAA CTCAGGCTTT GACTAATTAC GGAAGTGGAG AAGATGAGAA TGAAGATGAA 
GAAATAGAAG AATTTGAAGA GGGACCTGTA GATGTCCAGA CGTCACTTCA GGCTAACACT 
GAAACTACAG AAGAAAATGA ACACGATGAT CAGGTTCCAC AGCATGATTT TGAAAAATCA 
TCAGAAAGCA AAAATGTCCC ATCAGAACAA GAACCCACTA CTAGTAAAGA CTTTCTTTTG 
ATGACAGGTG ACCAGAATAG CACTCCTGTG AAGCCCTGTT ACCTCAGTAT CGTGGAAAAT 
GAGCAACCTT TAAATAGCCC TGTCCAGAAG GACACTCTCA CTACCATCGA CTCATCCAGT 
CAGCCTAACC CTTTGCCTTT ACCTTTAACT GAAATTGAAA CCTTGGTGCC TAGAGTCAAA 
GAAGTGAAAT CTGCTCAGGA GACTCCTGAA AGTTCTTTGG CTGGAAGTCC TGATACTGAA 
TCTCCAGTGT TAGTGAATGA CTATGAAGCA GAATCTGGTA ACATAAGTCA AAAATCTGAT 
GAAGAAGACT TTGTGAAAGT TGAAGATTTA CCACTTAAAC TGACAATATA TTCAGAGGCA 
GATCTAAGGA AGAAAATGGC CGAAGAAGAA CAGAAAAACC ACTTATCTGG TGAAATATGT 
GCAATGCAGA CTGAAGAATT AGCTGGGAAT TCTCAGACAC TAAAAGAACC TGAAATAGGG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( XM_025279471.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025135256.1 Bubalus bubalis pericentriolar material 1 (PCM1), transcript variant X11, mRNA.

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CloneID	OBu42299
Clone ID Related Accession (Same CDS sequence)	XM_025279471.1
Accession Version	XM_025279471.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6540bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	pericentriolar material 1 protein isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037545.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG GAGGAGGTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
AGCAATGAGA GTGTTGATGA CCGACTCAAC AATATGGATT GGGGCAGCCA GCAGAAGAAG 
GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA 
ACTAACGATA TTTCTCCGGA GTCATCACCG GGAGTTGGAA GACGAAGAAC AAAGACTTCA 
CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TCCCCGAGCA GGCAGAACTA 
GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCTCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCCAC TGTAAGTCCT 
CAAAAGAGAG AAATGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAGATTCGTG AGTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGACCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTCACT 
CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT CCAGAAGATT 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCGGT CTGAGGCTTC AGATGCCACG 
GAGGCATCTT TTAGTCTGAG AATTCGGCCG TGCGTTGAGG ACAAACTAGG GAATTCAGCT 
TCACAAGCAC AGGTTTCAGA CACTGACGTT ACTACAAGTC CAAAAGGGAA AGGTGACAGA 
CCTCCTCAGC ATGACAGGGA GCTGAGGCCT AATAGGAAAT GTAGCCGAAA ACGTGGATTT 
CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATGGAAG AGATAGAAAA TTTGAAGAAA 
CAACATGATT TATTAAAAAG GATGTTACAG CAGCAGGAGC AGCTGAGAGC TCTACAAGGA 
CGACAAGCTG CTCTGCTTGC CCTGCAGCAT AAAGCTGAGC AGGCTATAGC TGTGATGGAT 
GATTCTGAAA AGGTTGCGGA GACAACTCCT GGCCATCATA CTGTACCAGG CAGACAGCCG 
GCTCATTCAC CTTTGCATCA GAGAGTACCC ATAAGAGAAA CTACAGGTAG CCTATCTGGA 
GTCAGTATCA CCTCTGAACT AAACGAAGAA TTGAATGATT TAATTCAGCA TTTTCATAAT 
CAGCTTCGTG ATTCTCAGCC TCCAGCTGTT CCAGACAATA GAAGACAAGC AGAAAGTCTT 
TCATTAACTA GGGAGATTTC TCAGAGCAGA AATCCTTCAG TTTCAGAGCA TTTACCTGAT 
GAGAAAGTCC AACTTTTTAG CAAGATGAGA GTGTTACAAG AAAAGAAACA AAAAATGGAC 
AAACTTCTTG GAGAACTTCA TACACTTCGA GATCAGCATT TGAACAATTC ATCATTTGTG 
CCTTCTTCAG CCTCTCCACA AAGGAGTGTG GATCAGAGAA GTACAACCAC AGCTCCTTCT 
GCTCCTGTAG TCTTAACACC AGTGGTCAAT GGAGAATCCA ATAACCTCAC GCCATCTGTT 
CCTTATCCTG CTGCTTCTCT AGTTTCTCAG AATCAGAATG AAAATGAAGG CCACCTAAAT 
CCAACTGAAA AACTCCAGAA GTTAAATGAA GTTCGAAAGA GGTTGAATGA ACTAAGAGAA 
TTAGTTCATT ATTATGAGCA AACATCAGAT ATGATGACAG ATGCTGTGAA TGAAAACACA 
AAAGAAGAAG AAACTGAAGA ATCAGAATAT GATTCGGAGC ATGAAAACTC GGAACCTGTT 
ACTAACATTC GAAATCCACA AGTGGCTGCC ACCTGGAACG AAGTAAATAG TAATTCTAAT 
GCACAGTGTG TTTCCAACAA TAGAGATGGG CGAGCAGTTA ATTCTAACTG TGAAATTAAC 
AACAGGTCTG CTGCCAACAT AAGGGCTCTA AACATGCCTC CTCTTTCAGC AGATTGTCGA 
TACAATAGAG AAGGAGAACA GGAGATGCCT GTTGCACAAG GTCATGAGGA GGAGGAGGAG 
GAGGAGGAGG AAGCAGAAGA TGAGGCCCTC AGTGGAGCTT CACTGTCTAG TCACAGCAGC 
AGCCTGATCA TTGAGACTCC TGAAGATGCT GAATTTGAAC ATAAGATCCA GAAGCTTATG 
GCTGCAAAAC TGAAACTTAG ACACTTACAG AACCTTGTAG CCATGGTGCA GGACGATGAT 
GCAGCCAATC AAGGAGTTAC TTCTGTGGAG GATTTCTTCC CAGCAGAAGA CACCAAACAA 
AATGCAAATA ACACTAGAGG AAATGCCAAT AAAACACAGA AAGATGCTGG AGTAAATGAA 
AAGGCAAGGG AGAAATTTTA TGAGGCTAAA CTACAGCAGC AACAGAGAGA ACTCAAACAA 
TTGCAGGAAG AAAGAAAGAA ACTGATTGAG ATTCAAGAGA AAATTCAAGA ATTGCAAAAG 
GCATGTCCTA ACCTACAGCT GTCAGCTGCT AGTGTGGGTA ATTGTCCCAC AAAAACACAT 
ATGCCAGCTG TTACTTCATC CCCAACCGTT AATGAAAATG AAGCCAGTAC AAACAAATCT 
GGTTTTGAGC CTCATGATTC TTCAGTGGTA GATAATGAGG TATGGTCAGA TATGCGAAGA 
CATGAAATGT TAAGGGAGGA GCTACGACAG AGAAGAAAGC AGCTGGAAGC TCTGATGGCT 
GAACATCAGA GGAGGCAAGG TCTGGCTGAA ACTGCATCTC CAGTGGCTGT TTCACTGAGA 
AGTGATGGAT CTGAGAACCT GTGTACACCT CAGCAAAGCA GAACAGAAAA AACAATGGCA 
ACTTGGGGAG GTTCTACCCA GTGTGCCCTA GATGAAGAAG GAGATGAAGA TGGTTACCTT 
TCTGAAGGAA TTATTCGGAC AGACGAAGAG GAGGAAGAGG AGGAGCAAGA TGCCAGTTCC 
AATGATAACT TTCATATGTA TCCTCCAAAC AGTGCAAATC ATAATTCTTA CAATGTAAAG 
GAAACTAAAA ATAGGTGGAA GAACAATCGC CCTTTTTCAG CAGATGGAAA TTATCGTCCT 
TTAGCCAAGA CAAGACAGCA GAATATCAGC ATGCAGCGTC AGGAAAACCT TCGCTGGGTG 
TCCGAACTCT CTTATGTAGA AGAGAAAGAA CAATGGCAAG AACAAATTAA TCAGCTAAAG 
AAACAACTTG ATTTCAGTGT CAATATTTGT CAGACTTTAA TGCAAGACCA GCAGACTCTG 
TCTTGTCTGC TACAAACTCT TCTCACGAGT CCTTATAGTG TTATGCCCAG CAGTGTTGCA 
TCCCCGCAAG TGCACCTTAT AATGCACCAG TTGAATCAGT GCTATACCCA GCTAACGTGG 
CAGCAGAACA ATGTCCAGAG GTTGAAACAA ATGCTCAGTG AACTCATGCG CCAACAAAAT 
CAGCATCCAG AAAAACCTGC AAGCAAGGAA GGAGGCAGTG GTACATCACA CCCTCCTTCT 
CCCAGTTTAT TTTGTCCTTT CAACTTTCCA ACTCAGCCTG TAAATCTGTT TAACCTACCG 
GGGTTTACTA ATTTTTCGTC ATTTGCACCA AGTATGAATT TCAGCCCTTT ATTTCCTTCT 
AATTTTGGAG ATTTTTCTCA AAATATTTCT ACACCCACTG AACAGCAGCA ACCGTTAGCC 
CAGAATCCTT CAGGGAAAAC GGAATACATG GCTTTTCCAA AACCTTTTGA AAGTAGTTCT 
TCTATTGGAG CGGAAAAACA AAGGAATCAA AAACAGCCTG AAGAGGAGGT GGAGAACAGT 
AGGACACCAT GGTTTTATGA CCAAGAAGGT GAAGTAGAAA AGCCATTTCT CAAGACTGGA 
TTTGCAGTGT CTGTAGAGAA AACAACAAAT AGTAATCGCA AAAATCAACT AGATACCAGT 
AGGAGAAGAC GCCAGTTTGA TGAAGTGTCC TTAGAAAGCT TTAACAGTAT GCTTGACCCA 
GTAGATCCAA CAACAGTAAC TAAAACATTC AAGTCAAGAA AAGCATCTGC ACAGGCCAGC 
CTGGCATCTA AAGATAAAAC TCCCAAATCA AAGAGTAAGA AGAGGCATTC TACTCAGCTG 
AAAAGCAGAG TGAAAAATAT CGGGTATGAA AGTGCCAGTG TGTCTAGCAC ATGTGAACCT 
TGCAAAAGTA GAAACAGACA TTCAGCCCAA ACTGAAGAGC CTGTTCAAGC AAAAGTACTC 
AGCAGAAAGA ATCATGAGCA GCTGGAAAAA GTCATAAGAT GTAGTAGGTC TACAGAAATA 
TCTTCAGCAC ATGCTAGGAG AATACTACAG CAGTCTAACA GAAATGCATG CAATGAAGCG 
CCAGAAACCG GGAGTGATTT TTCCATGTTC GAAGCTTTGC GGGATACCAT TTATTCTGAA 
GTAGCTACGT TGATTTCTCA AAATGAATCT CGTCCACATT TTCTTATTGA ACTCTTCCAT 
GAGCTTCAGC TACTTAATAC AGACTATTTG AGACAGAGGG CCTTATATGC ATTGCAGGAC 
ATAGTATCCA GGCATATTTC TGAGAACCAT GAAAAAGAAG GAGAAAATAT AAAGTCCGTG 
AATTCTGGTA CTTGGATAGC ATCCAACTCA GAACTTACTC CCAGTGAAAG CCTTGCTACT 
ACTGATGATG AAACTTTTGA GAAGAACTTT GAGAGAGAAA CACATAAAAT AAGTGAGCAA 
AATGATGCTG ATAATGCTAG TGTATCTTCA ACTTGTGAGC CTTTTGCAAC AGATGTTCTG 
GGCGACACTG TGATTCACTT AGATCAGGCA TTAGCCAGAA TGAGAGACTA TACGCATATG 
AAGATGGAGG CTGAAAGTAG TCCAGACGTC AGGTGCACCT GCAGGATCAT TGAGGATGAC 
GATGGTGCTG CTGCCACAAC CACGATTAGT AATAATTTAG AAGAAACTCC TGTTATTGAA 
AATCATAGTT CACAGCAACC CGTGAGTGAA GTTGCTACCG TCCCGTGTCC TAGAATTGAT 
ACTCAGCAAC TGGACCGGCA AATTAAAGCA ATTATGAAAG AAGTCATTCC TTTTTTGAAG 
GAGCACATGG ATGAAGTATG TTCTTCTCAA CTTCTAACCT CAGTAAGACG CATGGTTTTG 
GTCCTTACTC AGCAAAACAA TGAGAGCAAA GAGTTTGTAA AGTTCTTTCA TAAGCAGCTT 
GGAAGTATAT TACAGGATTC ACTCGCAAAA TTTGCTGGCA GAAAACTGAA GGATTGTGGA 
GAAGATCTTC TTGTAGAGAT ATCTGAAGTG TTATTTAATG AATTGGCTTT CTTTAAGCTC 
ATGCAAGATT TGGATAATAA TAGTGTAACT GTTAAACAGA GATGCAAAAG GAAAATAGAA 
GCTGCTGGAG TGATACAGTC TTATGCAAAA GAGGCCAAAA GGATTCTTGA AGGAGATCAC 
AGTTCACCTG CTGGAGAAAT TGATGATGAA GACAAAGACA AGGATGAGAC GGAAACAGTT 
AAGCAGACTC AAACGTCTGA GGTTTATGAT GGTGACGGCC CCAAAAATGT GAGATCTGAT 
GTTTCTGATC AAGAGGATGA TGAAGAAAGT GAAGAATGTC CAGTGTCTAT TAATTTGTCC 
AAAGCTGAAA CTCAGGCTTT GACTAATTAC GGAAGTGGAG AAGATGAGAA TGAAGATGAA 
GAAATAGAAG AATTTGAAGA GGGACCTGTA GATGTCCAGA CGTCACTTCA GGCTAACACT 
GAAACTACAG AAGAAAATGA ACACGATGAT CAGGTTCCAC AGCATGATTT TGAAAAATCA 
TCAGAAAGCA AAAATGTCCC ATCAGAACAA GAACCCACTA CTAGTAAAGG TGACCAGAAT 
AGCACTCCTG TGAAGCCCTG TTACCTCAGT ATCGTGGAAA ATGAGCAACC TTTAAATAGC 
CCTGTCCAGA AGGACACTCT CACTACCATC GACTCATCCA GTCAGCCTAA CCCTTTGCCT 
TTACCTTTAA CTGAAATTGA AACCTTGGTG CCTAGAGTCA AAGAAGTGAA ATCTGCTCAG 
GAGACTCCTG AAAGTTCTTT GGCTGGAAGT CCTGATACTG AATCTCCAGT GTTAGTGAAT 
GACTATGAAG CAGAATCTGG TAACATAAGT CAAAAATCTG ATGAAGAAGA CTTTGTGAAA 
GTTGAAGATT TACCACTTAA ACTGACAATA TATTCAGAGG CAGATCTAAG GAAGAAAATG 
GCCGAAGAAG AACAGAAAAA CCACTTATCT GGTGAAATAT GTGCAATGCA GACTGAAGAA 
TTAGCTGGGA ATTCTCAGAC ACTAAAAGAA CCTGAAACAG TGGGAGCCCA AAGTACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025135256.1
CDS	392..6931
Translation	MATGGGPFEEGVNDQDLPNWSNESVDDRLNNMDWGSQQKKANRSSEKNKRKFGVESDKRVTNDISPESSPGVGRRRTKTSLTFPHSRYMTQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDATVSPQKREMIGSAQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIREYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLQKILARENEEEDVRTIDSAVGSGSVAESTSLNIDVRSEASDATEASFSLRIRPCVEDKLGNSASQAQVSDTDVTTSPKGKGDRPPQHDRELRPNRKCSRKRGFPSKARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSEKVAETTPGHHTVPGRQPAHSPLHQRVPIRETTGSLSGVSITSELNEELNDLIQHFHNQLRDSQPPAVPDNRRQAESLSLTREISQSRNPSVSEHLPDEKVQLFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSFVPSSASPQRSVDQRSTTTAPSAPVVLTPVVNGESNNLTPSVPYPAASLVSQNQNENEGHLNPTEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENTKEEETEESEYDSEHENSEPVTNIRNPQVAATWNEVNSNSNAQCVSNNRDGRAVNSNCEINNRSAANIRALNMPPLSADCRYNREGEQEMPVAQGHEEEEEEEEEAEDEALSGASLSSHSSSLIIETPEDAEFEHKIQKLMAAKLKLRHLQNLVAMVQDDDAANQGVTSVEDFFPAEDTKQNANNTRGNANKTQKDAGVNEKAREKFYEAKLQQQQRELKQLQEERKKLIEIQEKIQELQKACPNLQLSAASVGNCPTKTHMPAVTSSPTVNENEASTNKSGFEPHDSSVVDNEVWSDMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETASPVAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGIIRTDEEEEEEEQDASSNDNFHMYPPNSANHNSYNVKETKNRWKNNRPFSADGNYRPLAKTRQQNISMQRQENLRWVSELSYVEEKEQWQEQINQLKKQLDFSVNICQTLMQDQQTLSCLLQTLLTSPYSVMPSSVASPQVHLIMHQLNQCYTQLTWQQNNVQRLKQMLSELMRQQNQHPEKPASKEGGSGTSHPPSPSLFCPFNFPTQPVNLFNLPGFTNFSSFAPSMNFSPLFPSNFGDFSQNISTPTEQQQPLAQNPSGKTEYMAFPKPFESSSSIGAEKQRNQKQPEEEVENSRTPWFYDQEGEVEKPFLKTGFAVSVEKTTNSNRKNQLDTSRRRRQFDEVSLESFNSMLDPVDPTTVTKTFKSRKASAQASLASKDKTPKSKSKKRHSTQLKSRVKNIGYESASVSSTCEPCKSRNRHSAQTEEPVQAKVLSRKNHEQLEKVIRCSRSTEISSAHARRILQQSNRNACNEAPETGSDFSMFEALRDTIYSEVATLISQNESRPHFLIELFHELQLLNTDYLRQRALYALQDIVSRHISENHEKEGENIKSVNSGTWIASNSELTPSESLATTDDETFEKNFERETHKISEQNDADNASVSSTCEPFATDVLGDTVIHLDQALARMRDYTHMKMEAESSPDVRCTCRIIEDDDGAAATTTISNNLEETPVIENHSSQQPVSEVATVPCPRIDTQQLDRQIKAIMKEVIPFLKEHMDEVCSSQLLTSVRRMVLVLTQQNNESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSVTVKQRCKRKIEAAGVIQSYAKEAKRILEGDHSSPAGEIDDEDKDKDETETVKQTQTSEVYDGDGPKNVRSDVSDQEDDEESEECPVSINLSKAETQALTNYGSGEDENEDEEIEEFEEGPVDVQTSLQANTETTEENEHDDQVPQHDFEKSSESKNVPSEQEPTTSKGDQNSTPVKPCYLSIVENEQPLNSPVQKDTLTTIDSSSQPNPLPLPLTEIETLVPRVKEVKSAQETPESSLAGSPDTESPVLVNDYEAESGNISQKSDEEDFVKVEDLPLKLTIYSEADLRKKMAEEEQKNHLSGEICAMQTEELAGNSQTLKEPETVGAQST

Target ORF information:

RefSeq Version	XM_025279471.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis pericentriolar material 1 (PCM1), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025279471.1

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
AGCAATGAGA GTGTTGATGA CCGACTCAAC AATATGGATT GGGGCAGCCA GCAGAAGAAG 
GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA 
ACTAACGATA TTTCTCCGGA GTCATCACCG GGAGTTGGAA GACGAAGAAC AAAGACTTCA 
CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TCCCCGAGCA GGCAGAACTA 
GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCTCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCCAC TGTAAGTCCT 
CAAAAGAGAG AAATGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAGATTCGTG AGTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGACCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTCACT 
CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT CCAGAAGATT 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCGGT CTGAGGCTTC AGATGCCACG 
GAGGCATCTT TTAGTCTGAG AATTCGGCCG TGCGTTGAGG ACAAACTAGG GAATTCAGCT 
TCACAAGCAC AGGTTTCAGA CACTGACGTT ACTACAAGTC CAAAAGGGAA AGGTGACAGA 
CCTCCTCAGC ATGACAGGGA GCTGAGGCCT AATAGGAAAT GTAGCCGAAA ACGTGGATTT 
CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATGGAAG AGATAGAAAA TTTGAAGAAA 
CAACATGATT TATTAAAAAG GATGTTACAG CAGCAGGAGC AGCTGAGAGC TCTACAAGGA 
CGACAAGCTG CTCTGCTTGC CCTGCAGCAT AAAGCTGAGC AGGCTATAGC TGTGATGGAT 
GATTCTGAAA AGGTTGCGGA GACAACTCCT GGCCATCATA CTGTACCAGG CAGACAGCCG 
GCTCATTCAC CTTTGCATCA GAGAGTACCC ATAAGAGAAA CTACAGGTAG CCTATCTGGA 
GTCAGTATCA CCTCTGAACT AAACGAAGAA TTGAATGATT TAATTCAGCA TTTTCATAAT 
CAGCTTCGTG ATTCTCAGCC TCCAGCTGTT CCAGACAATA GAAGACAAGC AGAAAGTCTT 
TCATTAACTA GGGAGATTTC TCAGAGCAGA AATCCTTCAG TTTCAGAGCA TTTACCTGAT 
GAGAAAGTCC AACTTTTTAG CAAGATGAGA GTGTTACAAG AAAAGAAACA AAAAATGGAC 
AAACTTCTTG GAGAACTTCA TACACTTCGA GATCAGCATT TGAACAATTC ATCATTTGTG 
CCTTCTTCAG CCTCTCCACA AAGGAGTGTG GATCAGAGAA GTACAACCAC AGCTCCTTCT 
GCTCCTGTAG TCTTAACACC AGTGGTCAAT GGAGAATCCA ATAACCTCAC GCCATCTGTT 
CCTTATCCTG CTGCTTCTCT AGTTTCTCAG AATCAGAATG AAAATGAAGG CCACCTAAAT 
CCAACTGAAA AACTCCAGAA GTTAAATGAA GTTCGAAAGA GGTTGAATGA ACTAAGAGAA 
TTAGTTCATT ATTATGAGCA AACATCAGAT ATGATGACAG ATGCTGTGAA TGAAAACACA 
AAAGAAGAAG AAACTGAAGA ATCAGAATAT GATTCGGAGC ATGAAAACTC GGAACCTGTT 
ACTAACATTC GAAATCCACA AGTGGCTGCC ACCTGGAACG AAGTAAATAG TAATTCTAAT 
GCACAGTGTG TTTCCAACAA TAGAGATGGG CGAGCAGTTA ATTCTAACTG TGAAATTAAC 
AACAGGTCTG CTGCCAACAT AAGGGCTCTA AACATGCCTC CTCTTTCAGC AGATTGTCGA 
TACAATAGAG AAGGAGAACA GGAGATGCCT GTTGCACAAG GTCATGAGGA GGAGGAGGAG 
GAGGAGGAGG AAGCAGAAGA TGAGGCCCTC AGTGGAGCTT CACTGTCTAG TCACAGCAGC 
AGCCTGATCA TTGAGACTCC TGAAGATGCT GAATTTGAAC ATAAGATCCA GAAGCTTATG 
GCTGCAAAAC TGAAACTTAG ACACTTACAG AACCTTGTAG CCATGGTGCA GGACGATGAT 
GCAGCCAATC AAGGAGTTAC TTCTGTGGAG GATTTCTTCC CAGCAGAAGA CACCAAACAA 
AATGCAAATA ACACTAGAGG AAATGCCAAT AAAACACAGA AAGATGCTGG AGTAAATGAA 
AAGGCAAGGG AGAAATTTTA TGAGGCTAAA CTACAGCAGC AACAGAGAGA ACTCAAACAA 
TTGCAGGAAG AAAGAAAGAA ACTGATTGAG ATTCAAGAGA AAATTCAAGA ATTGCAAAAG 
GCATGTCCTA ACCTACAGCT GTCAGCTGCT AGTGTGGGTA ATTGTCCCAC AAAAACACAT 
ATGCCAGCTG TTACTTCATC CCCAACCGTT AATGAAAATG AAGCCAGTAC AAACAAATCT 
GGTTTTGAGC CTCATGATTC TTCAGTGGTA GATAATGAGG TATGGTCAGA TATGCGAAGA 
CATGAAATGT TAAGGGAGGA GCTACGACAG AGAAGAAAGC AGCTGGAAGC TCTGATGGCT 
GAACATCAGA GGAGGCAAGG TCTGGCTGAA ACTGCATCTC CAGTGGCTGT TTCACTGAGA 
AGTGATGGAT CTGAGAACCT GTGTACACCT CAGCAAAGCA GAACAGAAAA AACAATGGCA 
ACTTGGGGAG GTTCTACCCA GTGTGCCCTA GATGAAGAAG GAGATGAAGA TGGTTACCTT 
TCTGAAGGAA TTATTCGGAC AGACGAAGAG GAGGAAGAGG AGGAGCAAGA TGCCAGTTCC 
AATGATAACT TTCATATGTA TCCTCCAAAC AGTGCAAATC ATAATTCTTA CAATGTAAAG 
GAAACTAAAA ATAGGTGGAA GAACAATCGC CCTTTTTCAG CAGATGGAAA TTATCGTCCT 
TTAGCCAAGA CAAGACAGCA GAATATCAGC ATGCAGCGTC AGGAAAACCT TCGCTGGGTG 
TCCGAACTCT CTTATGTAGA AGAGAAAGAA CAATGGCAAG AACAAATTAA TCAGCTAAAG 
AAACAACTTG ATTTCAGTGT CAATATTTGT CAGACTTTAA TGCAAGACCA GCAGACTCTG 
TCTTGTCTGC TACAAACTCT TCTCACGAGT CCTTATAGTG TTATGCCCAG CAGTGTTGCA 
TCCCCGCAAG TGCACCTTAT AATGCACCAG TTGAATCAGT GCTATACCCA GCTAACGTGG 
CAGCAGAACA ATGTCCAGAG GTTGAAACAA ATGCTCAGTG AACTCATGCG CCAACAAAAT 
CAGCATCCAG AAAAACCTGC AAGCAAGGAA GGAGGCAGTG GTACATCACA CCCTCCTTCT 
CCCAGTTTAT TTTGTCCTTT CAACTTTCCA ACTCAGCCTG TAAATCTGTT TAACCTACCG 
GGGTTTACTA ATTTTTCGTC ATTTGCACCA AGTATGAATT TCAGCCCTTT ATTTCCTTCT 
AATTTTGGAG ATTTTTCTCA AAATATTTCT ACACCCACTG AACAGCAGCA ACCGTTAGCC 
CAGAATCCTT CAGGGAAAAC GGAATACATG GCTTTTCCAA AACCTTTTGA AAGTAGTTCT 
TCTATTGGAG CGGAAAAACA AAGGAATCAA AAACAGCCTG AAGAGGAGGT GGAGAACAGT 
AGGACACCAT GGTTTTATGA CCAAGAAGGT GAAGTAGAAA AGCCATTTCT CAAGACTGGA 
TTTGCAGTGT CTGTAGAGAA AACAACAAAT AGTAATCGCA AAAATCAACT AGATACCAGT 
AGGAGAAGAC GCCAGTTTGA TGAAGTGTCC TTAGAAAGCT TTAACAGTAT GCTTGACCCA 
GTAGATCCAA CAACAGTAAC TAAAACATTC AAGTCAAGAA AAGCATCTGC ACAGGCCAGC 
CTGGCATCTA AAGATAAAAC TCCCAAATCA AAGAGTAAGA AGAGGCATTC TACTCAGCTG 
AAAAGCAGAG TGAAAAATAT CGGGTATGAA AGTGCCAGTG TGTCTAGCAC ATGTGAACCT 
TGCAAAAGTA GAAACAGACA TTCAGCCCAA ACTGAAGAGC CTGTTCAAGC AAAAGTACTC 
AGCAGAAAGA ATCATGAGCA GCTGGAAAAA GTCATAAGAT GTAGTAGGTC TACAGAAATA 
TCTTCAGCAC ATGCTAGGAG AATACTACAG CAGTCTAACA GAAATGCATG CAATGAAGCG 
CCAGAAACCG GGAGTGATTT TTCCATGTTC GAAGCTTTGC GGGATACCAT TTATTCTGAA 
GTAGCTACGT TGATTTCTCA AAATGAATCT CGTCCACATT TTCTTATTGA ACTCTTCCAT 
GAGCTTCAGC TACTTAATAC AGACTATTTG AGACAGAGGG CCTTATATGC ATTGCAGGAC 
ATAGTATCCA GGCATATTTC TGAGAACCAT GAAAAAGAAG GAGAAAATAT AAAGTCCGTG 
AATTCTGGTA CTTGGATAGC ATCCAACTCA GAACTTACTC CCAGTGAAAG CCTTGCTACT 
ACTGATGATG AAACTTTTGA GAAGAACTTT GAGAGAGAAA CACATAAAAT AAGTGAGCAA 
AATGATGCTG ATAATGCTAG TGTATCTTCA ACTTGTGAGC CTTTTGCAAC AGATGTTCTG 
GGCGACACTG TGATTCACTT AGATCAGGCA TTAGCCAGAA TGAGAGACTA TACGCATATG 
AAGATGGAGG CTGAAAGTAG TCCAGACGTC AGGTGCACCT GCAGGATCAT TGAGGATGAC 
GATGGTGCTG CTGCCACAAC CACGATTAGT AATAATTTAG AAGAAACTCC TGTTATTGAA 
AATCATAGTT CACAGCAACC CGTGAGTGAA GTTGCTACCG TCCCGTGTCC TAGAATTGAT 
ACTCAGCAAC TGGACCGGCA AATTAAAGCA ATTATGAAAG AAGTCATTCC TTTTTTGAAG 
GAGCACATGG ATGAAGTATG TTCTTCTCAA CTTCTAACCT CAGTAAGACG CATGGTTTTG 
GTCCTTACTC AGCAAAACAA TGAGAGCAAA GAGTTTGTAA AGTTCTTTCA TAAGCAGCTT 
GGAAGTATAT TACAGGATTC ACTCGCAAAA TTTGCTGGCA GAAAACTGAA GGATTGTGGA 
GAAGATCTTC TTGTAGAGAT ATCTGAAGTG TTATTTAATG AATTGGCTTT CTTTAAGCTC 
ATGCAAGATT TGGATAATAA TAGTGTAACT GTTAAACAGA GATGCAAAAG GAAAATAGAA 
GCTGCTGGAG TGATACAGTC TTATGCAAAA GAGGCCAAAA GGATTCTTGA AGGAGATCAC 
AGTTCACCTG CTGGAGAAAT TGATGATGAA GACAAAGACA AGGATGAGAC GGAAACAGTT 
AAGCAGACTC AAACGTCTGA GGTTTATGAT GGTGACGGCC CCAAAAATGT GAGATCTGAT 
GTTTCTGATC AAGAGGATGA TGAAGAAAGT GAAGAATGTC CAGTGTCTAT TAATTTGTCC 
AAAGCTGAAA CTCAGGCTTT GACTAATTAC GGAAGTGGAG AAGATGAGAA TGAAGATGAA 
GAAATAGAAG AATTTGAAGA GGGACCTGTA GATGTCCAGA CGTCACTTCA GGCTAACACT 
GAAACTACAG AAGAAAATGA ACACGATGAT CAGGTTCCAC AGCATGATTT TGAAAAATCA 
TCAGAAAGCA AAAATGTCCC ATCAGAACAA GAACCCACTA CTAGTAAAGG TGACCAGAAT 
AGCACTCCTG TGAAGCCCTG TTACCTCAGT ATCGTGGAAA ATGAGCAACC TTTAAATAGC 
CCTGTCCAGA AGGACACTCT CACTACCATC GACTCATCCA GTCAGCCTAA CCCTTTGCCT 
TTACCTTTAA CTGAAATTGA AACCTTGGTG CCTAGAGTCA AAGAAGTGAA ATCTGCTCAG 
GAGACTCCTG AAAGTTCTTT GGCTGGAAGT CCTGATACTG AATCTCCAGT GTTAGTGAAT 
GACTATGAAG CAGAATCTGG TAACATAAGT CAAAAATCTG ATGAAGAAGA CTTTGTGAAA 
GTTGAAGATT TACCACTTAA ACTGACAATA TATTCAGAGG CAGATCTAAG GAAGAAAATG 
GCCGAAGAAG AACAGAAAAA CCACTTATCT GGTGAAATAT GTGCAATGCA GACTGAAGAA 
TTAGCTGGGA ATTCTCAGAC ACTAAAAGAA CCTGAAACAG TGGGAGCCCA AAGTACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( XM_025279478.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025135263.1 Bubalus bubalis pericentriolar material 1 (PCM1), transcript variant X12, mRNA.

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CloneID	OBu42300
Clone ID Related Accession (Same CDS sequence)	XM_025279478.1
Accession Version	XM_025279478.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6498bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	pericentriolar material 1 protein isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037545.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG GAGGAGGTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
AGCAATGAGA GTGTTGATGA CCGACTCAAC AATATGGATT GGGGCAGCCA GCAGAAGAAG 
GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA 
ACTAACGATA TTTCTCCGGA GTCATCACCG GGAGTTGGAA GACGAAGAAC AAAGACTTCA 
CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TCCCCGAGCA GGCAGAACTA 
GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCTCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCCAC TGTAAGTCCT 
CAAAAGAGAG AAATGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAGATTCGTG AGTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGACCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTCACT 
CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT CCAGAAGATT 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCGGT CTGAGGCTTC AGATGCCACG 
GCCAGAGATC CTCAGCAGGA GCCTATGGAA GAGATAGAAA ATTTGAAGAA ACAACATGAT 
TTATTAAAAA GGATGTTACA GCAGCAGGAG CAGCTGAGAG CTCTACAAGG ACGACAAGCT 
GCTCTGCTTG CCCTGCAGCA TAAAGCTGAG CAGGCTATAG CTGTGATGGA TGATTCTGAA 
AAGGTTGCGG AGACAACTCC TGGCCATCAT ACTGTACCAG GCAGACAGCC GGCTCATTCA 
CCTTTGCATC AGAGAGTACC CATAAGAGAA ACTACAGGTA GCCTATCTGG AGTCAGTATC 
ACCTCTGAAC TAAACGAAGA ATTGAATGAT TTAATTCAGC ATTTTCATAA TCAGCTTCGT 
GATTCTCAGC CTCCAGCTGT TCCAGACAAT AGAAGACAAG CAGAAAGTCT TTCATTAACT 
AGGGAGATTT CTCAGAGCAG AAATCCTTCA GTTTCAGAGC ATTTACCTGA TGAGAAAGTC 
CAACTTTTTA GCAAGATGAG AGTGTTACAA GAAAAGAAAC AAAAAATGGA CAAACTTCTT 
GGAGAACTTC ATACACTTCG AGATCAGCAT TTGAACAATT CATCATTTGT GCCTTCTTCA 
GCCTCTCCAC AAAGGAGTGT GGATCAGAGA AGTACAACCA CAGCTCCTTC TGCTCCTGTA 
GTCTTAACAC CAGTGGTCAA TGGAGAATCC AATAACCTCA CGCCATCTGT TCCTTATCCT 
GCTGCTTCTC TAGTTTCTCA GAATCAGAAT GAAAATGAAG GCCACCTAAA TCCAACTGAA 
AAACTCCAGA AGTTAAATGA AGTTCGAAAG AGGTTGAATG AACTAAGAGA ATTAGTTCAT 
TATTATGAGC AAACATCAGA TATGATGACA GATGCTGTGA ATGAAAACAC AAAAGAAGAA 
GAAACTGAAG AATCAGAATA TGATTCGGAG CATGAAAACT CGGAACCTGT TACTAACATT 
CGAAATCCAC AAGTGGCTGC CACCTGGAAC GAAGTAAATA GTAATTCTAA TGCACAGTGT 
GTTTCCAACA ATAGAGATGG GCGAGCAGTT AATTCTAACT GTGAAATTAA CAACAGGTCT 
GCTGCCAACA TAAGGGCTCT AAACATGCCT CCTCTTTCAG CAGATTGTCG ATACAATAGA 
GAAGGAGAAC AGGAGATGCC TGTTGCACAA GGTCATGAGG AGGAGGAGGA GGAGGAGGAG 
GAAGCAGAAG ATGAGGCCCT CAGTGGAGCT TCACTGTCTA GTCACAGCAG CAGCCTGATC 
ATTGAGACTC CTGAAGATGC TGAATTTGAA CATAAGATCC AGAAGCTTAT GGCTGCAAAA 
CTGAAACTTA GACACTTACA GAACCTTGTA GCCATGGTGC AGGACGATGA TGCAGCCAAT 
CAAGGAGTTA CTTCTGTGGA GGATTTCTTC CCAGCAGAAG ACACCAAACA AAATGCAAAT 
AACACTAGAG GAAATGCCAA TAAAACACAG AAAGATGCTG GAGTAAATGA AAAGGCAAGG 
GAGAAATTTT ATGAGGCTAA ACTACAGCAG CAACAGAGAG AACTCAAACA ATTGCAGGAA 
GAAAGAAAGA AACTGATTGA GATTCAAGAG AAAATTCAAG AATTGCAAAA GGCATGTCCT 
AACCTACAGC TGTCAGCTGC TAGTGTGGGT AATTGTCCCA CAAAAACACA TATGCCAGCT 
GTTACTTCAT CCCCAACCGT TAATGAAAAT GAAGCCAGTA CAAACAAATC TGGTTTTGAG 
CCTCATGATT CTTCAGTGGT AGATAATGAG GTATGGTCAG ATATGCGAAG ACATGAAATG 
TTAAGGGAGG AGCTACGACA GAGAAGAAAG CAGCTGGAAG CTCTGATGGC TGAACATCAG 
AGGAGGCAAG GTCTGGCTGA AACTGCATCT CCAGTGGCTG TTTCACTGAG AAGTGATGGA 
TCTGAGAACC TGTGTACACC TCAGCAAAGC AGAACAGAAA AAACAATGGC AACTTGGGGA 
GGTTCTACCC AGTGTGCCCT AGATGAAGAA GGAGATGAAG ATGGTTACCT TTCTGAAGGA 
ATTATTCGGA CAGACGAAGA GGAGGAAGAG GAGGAGCAAG ATGCCAGTTC CAATGATAAC 
TTTCATATGT ATCCTCCAAA CAGTGCAAAT CATAATTCTT ACAATGTAAA GGAAACTAAA 
AATAGGTGGA AGAACAATCG CCCTTTTTCA GCAGATGGAA ATTATCGTCC TTTAGCCAAG 
ACAAGACAGC AGAATATCAG CATGCAGCGT CAGGAAAACC TTCGCTGGGT GTCCGAACTC 
TCTTATGTAG AAGAGAAAGA ACAATGGCAA GAACAAATTA ATCAGCTAAA GAAACAACTT 
GATTTCAGTG TCAATATTTG TCAGACTTTA ATGCAAGACC AGCAGACTCT GTCTTGTCTG 
CTACAAACTC TTCTCACGAG TCCTTATAGT GTTATGCCCA GCAGTGTTGC ATCCCCGCAA 
GTGCACCTTA TAATGCACCA GTTGAATCAG TGCTATACCC AGCTAACGTG GCAGCAGAAC 
AATGTCCAGA GGTTGAAACA AATGCTCAGT GAACTCATGC GCCAACAAAA TCAGCATCCA 
GAAAAACCTG CAAGCAAGGA AGGAGGCAGT GGTACATCAC ACCCTCCTTC TCCCAGTTTA 
TTTTGTCCTT TCAACTTTCC AACTCAGCCT GTAAATCTGT TTAACCTACC GGGGTTTACT 
AATTTTTCGT CATTTGCACC AAGTATGAAT TTCAGCCCTT TATTTCCTTC TAATTTTGGA 
GATTTTTCTC AAAATATTTC TACACCCACT GAACAGCAGC AACCGTTAGC CCAGAATCCT 
TCAGGGAAAA CGGAATACAT GGCTTTTCCA AAACCTTTTG AAAGTAGTTC TTCTATTGGA 
GCGGAAAAAC AAAGGAATCA AAAACAGCCT GAAGAGGAGG TGGAGAACAG TAGGACACCA 
TGGTTTTATG ACCAAGAAGG TGAAGTAGAA AAGCCATTTC TCAAGACTGG ATTTGCAGTG 
TCTGTAGAGA AAACAACAAA TAGTAATCGC AAAAATCAAC TAGATACCAG TAGGAGAAGA 
CGCCAGTTTG ATGAAGTGTC CTTAGAAAGC TTTAACAGTA TGCTTGACCC AGTAGATCCA 
ACAACAGTAA CTAAAACATT CAAGTCAAGA AAAGCATCTG CACAGGCCAG CCTGGCATCT 
AAAGATAAAA CTCCCAAATC AAAGAGTAAG AAGAGGCATT CTACTCAGCT GAAAAGCAGA 
GTGAAAAATA TCGGGTATGA AAGTGCCAGT GTGTCTAGCA CATGTGAACC TTGCAAAAGT 
AGAAACAGAC ATTCAGCCCA AACTGAAGAG CCTGTTCAAG CAAAAGTACT CAGCAGAAAG 
AATCATGAGC AGCTGGAAAA AGTCATAAGA TGTAGTAGGT CTACAGAAAT ATCTTCAGCA 
CATGCTAGGA GAATACTACA GCAGTCTAAC AGAAATGCAT GCAATGAAGC GCCAGAAACC 
GGGAGTGATT TTTCCATGTT CGAAGCTTTG CGGGATACCA TTTATTCTGA AGTAGCTACG 
TTGATTTCTC AAAATGAATC TCGTCCACAT TTTCTTATTG AACTCTTCCA TGAGCTTCAG 
CTACTTAATA CAGACTATTT GAGACAGAGG GCCTTATATG CATTGCAGGA CATAGTATCC 
AGGCATATTT CTGAGAACCA TGAAAAAGAA GGAGAAAATA TAAAGTCCGT GAATTCTGGT 
ACTTGGATAG CATCCAACTC AGAACTTACT CCCAGTGAAA GCCTTGCTAC TACTGATGAT 
GAAACTTTTG AGAAGAACTT TGAGAGAGAA ACACATAAAA TAAGTGAGCA AAATGATGCT 
GATAATGCTA GTGTATCTTC AACTTGTGAG CCTTTTGCAA CAGATGTTCT GGGCGACACT 
GTGATTCACT TAGATCAGGC ATTAGCCAGA ATGAGAGACT ATACGCATAT GAAGATGGAG 
GCTGAAAGTA GTCCAGACGT CAGGTGCACC TGCAGGATCA TTGAGGATGA CGATGGTGCT 
GCTGCCACAA CCACGATTAG TAATAATTTA GAAGAAACTC CTGTTATTGA AAATCATAGT 
TCACAGCAAC CCGTGAGTGA AGTTGCTACC GTCCCGTGTC CTAGAATTGA TACTCAGCAA 
CTGGACCGGC AAATTAAAGC AATTATGAAA GAAGTCATTC CTTTTTTGAA GGAGCACATG 
GATGAAGTAT GTTCTTCTCA ACTTCTAACC TCAGTAAGAC GCATGGTTTT GGTCCTTACT 
CAGCAAAACA ATGAGAGCAA AGAGTTTGTA AAGTTCTTTC ATAAGCAGCT TGGAAGTATA 
TTACAGGATT CACTCGCAAA ATTTGCTGGC AGAAAACTGA AGGATTGTGG AGAAGATCTT 
CTTGTAGAGA TATCTGAAGT GTTATTTAAT GAATTGGCTT TCTTTAAGCT CATGCAAGAT 
TTGGATAATA ATAGTGTAAC TGTTAAACAG AGATGCAAAA GGAAAATAGA AGCTGCTGGA 
GTGATACAGT CTTATGCAAA AGAGGCCAAA AGGATTCTTG AAGGAGATCA CAGTTCACCT 
GCTGGAGAAA TTGATGATGA AGACAAAGAC AAGGATGAGA CGGAAACAGT TAAGCAGACT 
CAAACGTCTG AGGTTTATGA TGGTGACGGC CCCAAAAATG TGAGATCTGA TGTTTCTGAT 
CAAGAGGATG ATGAAGAAAG TGAAGAATGT CCAGTGTCTA TTAATTTGTC CAAAGCTGAA 
ACTCAGGCTT TGACTAATTA CGGAAGTGGA GAAGATGAGA ATGAAGATGA AGAAATAGAA 
GAATTTGAAG AGGGACCTGT AGATGTCCAG ACGTCACTTC AGGCTAACAC TGAAACTACA 
GAAGAAAATG AACACGATGA TCAGGTTCCA CAGCATGATT TTGAAAAATC ATCAGAAAGC 
AAAAATGTCC CATCAGAACA AGAACCCACT ACTAGTAAAG ACTTTCTTTT GATGACAGGT 
GACCAGAATA GCACTCCTGT GAAGCCCTGT TACCTCAGTA TCGTGGAAAA TGAGCAACCT 
TTAAATAGCC CTGTCCAGAA GGACACTCTC ACTACCATCG ACTCATCCAG TCAGCCTAAC 
CCTTTGCCTT TACCTTTAAC TGAAATTGAA ACCTTGGTGC CTAGAGTCAA AGAAGTGAAA 
TCTGCTCAGG AGACTCCTGA AAGTTCTTTG GCTGGAAGTC CTGATACTGA ATCTCCAGTG 
TTAGTGAATG ACTATGAAGC AGAATCTGGT AACATAAGTC AAAAATCTGA TGAAGAAGAC 
TTTGTGAAAG TTGAAGATTT ACCACTTAAA CTGACAATAT ATTCAGAGGC AGATCTAAGG 
AAGAAAATGG CCGAAGAAGA ACAGAAAAAC CACTTATCTG GTGAAATATG TGCAATGCAG 
ACTGAAGAAT TAGCTGGGAA TTCTCAGACA CTAAAAGAAC CTGAGCAAGT ATGTGCTCAG 
TCTGCCATCT TAAGATGGAA ACCCTTACCT GTGCCTTTTA AACTGTTGGT GGTGTCATCA 
GGGAATTATT GTAGCAAAAA ACAGTGGGAG CCCAAAGTAC ATGAGATGTC TCCAGAGGCT 
CAGCTAACTC TTTCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025135263.1
CDS	392..6889
Translation	MATGGGPFEEGVNDQDLPNWSNESVDDRLNNMDWGSQQKKANRSSEKNKRKFGVESDKRVTNDISPESSPGVGRRRTKTSLTFPHSRYMTQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDATVSPQKREMIGSAQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIREYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLQKILARENEEEDVRTIDSAVGSGSVAESTSLNIDVRSEASDATARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSEKVAETTPGHHTVPGRQPAHSPLHQRVPIRETTGSLSGVSITSELNEELNDLIQHFHNQLRDSQPPAVPDNRRQAESLSLTREISQSRNPSVSEHLPDEKVQLFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSFVPSSASPQRSVDQRSTTTAPSAPVVLTPVVNGESNNLTPSVPYPAASLVSQNQNENEGHLNPTEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENTKEEETEESEYDSEHENSEPVTNIRNPQVAATWNEVNSNSNAQCVSNNRDGRAVNSNCEINNRSAANIRALNMPPLSADCRYNREGEQEMPVAQGHEEEEEEEEEAEDEALSGASLSSHSSSLIIETPEDAEFEHKIQKLMAAKLKLRHLQNLVAMVQDDDAANQGVTSVEDFFPAEDTKQNANNTRGNANKTQKDAGVNEKAREKFYEAKLQQQQRELKQLQEERKKLIEIQEKIQELQKACPNLQLSAASVGNCPTKTHMPAVTSSPTVNENEASTNKSGFEPHDSSVVDNEVWSDMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETASPVAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGIIRTDEEEEEEEQDASSNDNFHMYPPNSANHNSYNVKETKNRWKNNRPFSADGNYRPLAKTRQQNISMQRQENLRWVSELSYVEEKEQWQEQINQLKKQLDFSVNICQTLMQDQQTLSCLLQTLLTSPYSVMPSSVASPQVHLIMHQLNQCYTQLTWQQNNVQRLKQMLSELMRQQNQHPEKPASKEGGSGTSHPPSPSLFCPFNFPTQPVNLFNLPGFTNFSSFAPSMNFSPLFPSNFGDFSQNISTPTEQQQPLAQNPSGKTEYMAFPKPFESSSSIGAEKQRNQKQPEEEVENSRTPWFYDQEGEVEKPFLKTGFAVSVEKTTNSNRKNQLDTSRRRRQFDEVSLESFNSMLDPVDPTTVTKTFKSRKASAQASLASKDKTPKSKSKKRHSTQLKSRVKNIGYESASVSSTCEPCKSRNRHSAQTEEPVQAKVLSRKNHEQLEKVIRCSRSTEISSAHARRILQQSNRNACNEAPETGSDFSMFEALRDTIYSEVATLISQNESRPHFLIELFHELQLLNTDYLRQRALYALQDIVSRHISENHEKEGENIKSVNSGTWIASNSELTPSESLATTDDETFEKNFERETHKISEQNDADNASVSSTCEPFATDVLGDTVIHLDQALARMRDYTHMKMEAESSPDVRCTCRIIEDDDGAAATTTISNNLEETPVIENHSSQQPVSEVATVPCPRIDTQQLDRQIKAIMKEVIPFLKEHMDEVCSSQLLTSVRRMVLVLTQQNNESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSVTVKQRCKRKIEAAGVIQSYAKEAKRILEGDHSSPAGEIDDEDKDKDETETVKQTQTSEVYDGDGPKNVRSDVSDQEDDEESEECPVSINLSKAETQALTNYGSGEDENEDEEIEEFEEGPVDVQTSLQANTETTEENEHDDQVPQHDFEKSSESKNVPSEQEPTTSKDFLLMTGDQNSTPVKPCYLSIVENEQPLNSPVQKDTLTTIDSSSQPNPLPLPLTEIETLVPRVKEVKSAQETPESSLAGSPDTESPVLVNDYEAESGNISQKSDEEDFVKVEDLPLKLTIYSEADLRKKMAEEEQKNHLSGEICAMQTEELAGNSQTLKEPEQVCAQSAILRWKPLPVPFKLLVVSSGNYCSKKQWEPKVHEMSPEAQLTLS

Target ORF information:

RefSeq Version	XM_025279478.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis pericentriolar material 1 (PCM1), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025279478.1

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
AGCAATGAGA GTGTTGATGA CCGACTCAAC AATATGGATT GGGGCAGCCA GCAGAAGAAG 
GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA 
ACTAACGATA TTTCTCCGGA GTCATCACCG GGAGTTGGAA GACGAAGAAC AAAGACTTCA 
CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TCCCCGAGCA GGCAGAACTA 
GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCTCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCCAC TGTAAGTCCT 
CAAAAGAGAG AAATGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAGATTCGTG AGTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGACCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTCACT 
CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT CCAGAAGATT 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCGGT CTGAGGCTTC AGATGCCACG 
GCCAGAGATC CTCAGCAGGA GCCTATGGAA GAGATAGAAA ATTTGAAGAA ACAACATGAT 
TTATTAAAAA GGATGTTACA GCAGCAGGAG CAGCTGAGAG CTCTACAAGG ACGACAAGCT 
GCTCTGCTTG CCCTGCAGCA TAAAGCTGAG CAGGCTATAG CTGTGATGGA TGATTCTGAA 
AAGGTTGCGG AGACAACTCC TGGCCATCAT ACTGTACCAG GCAGACAGCC GGCTCATTCA 
CCTTTGCATC AGAGAGTACC CATAAGAGAA ACTACAGGTA GCCTATCTGG AGTCAGTATC 
ACCTCTGAAC TAAACGAAGA ATTGAATGAT TTAATTCAGC ATTTTCATAA TCAGCTTCGT 
GATTCTCAGC CTCCAGCTGT TCCAGACAAT AGAAGACAAG CAGAAAGTCT TTCATTAACT 
AGGGAGATTT CTCAGAGCAG AAATCCTTCA GTTTCAGAGC ATTTACCTGA TGAGAAAGTC 
CAACTTTTTA GCAAGATGAG AGTGTTACAA GAAAAGAAAC AAAAAATGGA CAAACTTCTT 
GGAGAACTTC ATACACTTCG AGATCAGCAT TTGAACAATT CATCATTTGT GCCTTCTTCA 
GCCTCTCCAC AAAGGAGTGT GGATCAGAGA AGTACAACCA CAGCTCCTTC TGCTCCTGTA 
GTCTTAACAC CAGTGGTCAA TGGAGAATCC AATAACCTCA CGCCATCTGT TCCTTATCCT 
GCTGCTTCTC TAGTTTCTCA GAATCAGAAT GAAAATGAAG GCCACCTAAA TCCAACTGAA 
AAACTCCAGA AGTTAAATGA AGTTCGAAAG AGGTTGAATG AACTAAGAGA ATTAGTTCAT 
TATTATGAGC AAACATCAGA TATGATGACA GATGCTGTGA ATGAAAACAC AAAAGAAGAA 
GAAACTGAAG AATCAGAATA TGATTCGGAG CATGAAAACT CGGAACCTGT TACTAACATT 
CGAAATCCAC AAGTGGCTGC CACCTGGAAC GAAGTAAATA GTAATTCTAA TGCACAGTGT 
GTTTCCAACA ATAGAGATGG GCGAGCAGTT AATTCTAACT GTGAAATTAA CAACAGGTCT 
GCTGCCAACA TAAGGGCTCT AAACATGCCT CCTCTTTCAG CAGATTGTCG ATACAATAGA 
GAAGGAGAAC AGGAGATGCC TGTTGCACAA GGTCATGAGG AGGAGGAGGA GGAGGAGGAG 
GAAGCAGAAG ATGAGGCCCT CAGTGGAGCT TCACTGTCTA GTCACAGCAG CAGCCTGATC 
ATTGAGACTC CTGAAGATGC TGAATTTGAA CATAAGATCC AGAAGCTTAT GGCTGCAAAA 
CTGAAACTTA GACACTTACA GAACCTTGTA GCCATGGTGC AGGACGATGA TGCAGCCAAT 
CAAGGAGTTA CTTCTGTGGA GGATTTCTTC CCAGCAGAAG ACACCAAACA AAATGCAAAT 
AACACTAGAG GAAATGCCAA TAAAACACAG AAAGATGCTG GAGTAAATGA AAAGGCAAGG 
GAGAAATTTT ATGAGGCTAA ACTACAGCAG CAACAGAGAG AACTCAAACA ATTGCAGGAA 
GAAAGAAAGA AACTGATTGA GATTCAAGAG AAAATTCAAG AATTGCAAAA GGCATGTCCT 
AACCTACAGC TGTCAGCTGC TAGTGTGGGT AATTGTCCCA CAAAAACACA TATGCCAGCT 
GTTACTTCAT CCCCAACCGT TAATGAAAAT GAAGCCAGTA CAAACAAATC TGGTTTTGAG 
CCTCATGATT CTTCAGTGGT AGATAATGAG GTATGGTCAG ATATGCGAAG ACATGAAATG 
TTAAGGGAGG AGCTACGACA GAGAAGAAAG CAGCTGGAAG CTCTGATGGC TGAACATCAG 
AGGAGGCAAG GTCTGGCTGA AACTGCATCT CCAGTGGCTG TTTCACTGAG AAGTGATGGA 
TCTGAGAACC TGTGTACACC TCAGCAAAGC AGAACAGAAA AAACAATGGC AACTTGGGGA 
GGTTCTACCC AGTGTGCCCT AGATGAAGAA GGAGATGAAG ATGGTTACCT TTCTGAAGGA 
ATTATTCGGA CAGACGAAGA GGAGGAAGAG GAGGAGCAAG ATGCCAGTTC CAATGATAAC 
TTTCATATGT ATCCTCCAAA CAGTGCAAAT CATAATTCTT ACAATGTAAA GGAAACTAAA 
AATAGGTGGA AGAACAATCG CCCTTTTTCA GCAGATGGAA ATTATCGTCC TTTAGCCAAG 
ACAAGACAGC AGAATATCAG CATGCAGCGT CAGGAAAACC TTCGCTGGGT GTCCGAACTC 
TCTTATGTAG AAGAGAAAGA ACAATGGCAA GAACAAATTA ATCAGCTAAA GAAACAACTT 
GATTTCAGTG TCAATATTTG TCAGACTTTA ATGCAAGACC AGCAGACTCT GTCTTGTCTG 
CTACAAACTC TTCTCACGAG TCCTTATAGT GTTATGCCCA GCAGTGTTGC ATCCCCGCAA 
GTGCACCTTA TAATGCACCA GTTGAATCAG TGCTATACCC AGCTAACGTG GCAGCAGAAC 
AATGTCCAGA GGTTGAAACA AATGCTCAGT GAACTCATGC GCCAACAAAA TCAGCATCCA 
GAAAAACCTG CAAGCAAGGA AGGAGGCAGT GGTACATCAC ACCCTCCTTC TCCCAGTTTA 
TTTTGTCCTT TCAACTTTCC AACTCAGCCT GTAAATCTGT TTAACCTACC GGGGTTTACT 
AATTTTTCGT CATTTGCACC AAGTATGAAT TTCAGCCCTT TATTTCCTTC TAATTTTGGA 
GATTTTTCTC AAAATATTTC TACACCCACT GAACAGCAGC AACCGTTAGC CCAGAATCCT 
TCAGGGAAAA CGGAATACAT GGCTTTTCCA AAACCTTTTG AAAGTAGTTC TTCTATTGGA 
GCGGAAAAAC AAAGGAATCA AAAACAGCCT GAAGAGGAGG TGGAGAACAG TAGGACACCA 
TGGTTTTATG ACCAAGAAGG TGAAGTAGAA AAGCCATTTC TCAAGACTGG ATTTGCAGTG 
TCTGTAGAGA AAACAACAAA TAGTAATCGC AAAAATCAAC TAGATACCAG TAGGAGAAGA 
CGCCAGTTTG ATGAAGTGTC CTTAGAAAGC TTTAACAGTA TGCTTGACCC AGTAGATCCA 
ACAACAGTAA CTAAAACATT CAAGTCAAGA AAAGCATCTG CACAGGCCAG CCTGGCATCT 
AAAGATAAAA CTCCCAAATC AAAGAGTAAG AAGAGGCATT CTACTCAGCT GAAAAGCAGA 
GTGAAAAATA TCGGGTATGA AAGTGCCAGT GTGTCTAGCA CATGTGAACC TTGCAAAAGT 
AGAAACAGAC ATTCAGCCCA AACTGAAGAG CCTGTTCAAG CAAAAGTACT CAGCAGAAAG 
AATCATGAGC AGCTGGAAAA AGTCATAAGA TGTAGTAGGT CTACAGAAAT ATCTTCAGCA 
CATGCTAGGA GAATACTACA GCAGTCTAAC AGAAATGCAT GCAATGAAGC GCCAGAAACC 
GGGAGTGATT TTTCCATGTT CGAAGCTTTG CGGGATACCA TTTATTCTGA AGTAGCTACG 
TTGATTTCTC AAAATGAATC TCGTCCACAT TTTCTTATTG AACTCTTCCA TGAGCTTCAG 
CTACTTAATA CAGACTATTT GAGACAGAGG GCCTTATATG CATTGCAGGA CATAGTATCC 
AGGCATATTT CTGAGAACCA TGAAAAAGAA GGAGAAAATA TAAAGTCCGT GAATTCTGGT 
ACTTGGATAG CATCCAACTC AGAACTTACT CCCAGTGAAA GCCTTGCTAC TACTGATGAT 
GAAACTTTTG AGAAGAACTT TGAGAGAGAA ACACATAAAA TAAGTGAGCA AAATGATGCT 
GATAATGCTA GTGTATCTTC AACTTGTGAG CCTTTTGCAA CAGATGTTCT GGGCGACACT 
GTGATTCACT TAGATCAGGC ATTAGCCAGA ATGAGAGACT ATACGCATAT GAAGATGGAG 
GCTGAAAGTA GTCCAGACGT CAGGTGCACC TGCAGGATCA TTGAGGATGA CGATGGTGCT 
GCTGCCACAA CCACGATTAG TAATAATTTA GAAGAAACTC CTGTTATTGA AAATCATAGT 
TCACAGCAAC CCGTGAGTGA AGTTGCTACC GTCCCGTGTC CTAGAATTGA TACTCAGCAA 
CTGGACCGGC AAATTAAAGC AATTATGAAA GAAGTCATTC CTTTTTTGAA GGAGCACATG 
GATGAAGTAT GTTCTTCTCA ACTTCTAACC TCAGTAAGAC GCATGGTTTT GGTCCTTACT 
CAGCAAAACA ATGAGAGCAA AGAGTTTGTA AAGTTCTTTC ATAAGCAGCT TGGAAGTATA 
TTACAGGATT CACTCGCAAA ATTTGCTGGC AGAAAACTGA AGGATTGTGG AGAAGATCTT 
CTTGTAGAGA TATCTGAAGT GTTATTTAAT GAATTGGCTT TCTTTAAGCT CATGCAAGAT 
TTGGATAATA ATAGTGTAAC TGTTAAACAG AGATGCAAAA GGAAAATAGA AGCTGCTGGA 
GTGATACAGT CTTATGCAAA AGAGGCCAAA AGGATTCTTG AAGGAGATCA CAGTTCACCT 
GCTGGAGAAA TTGATGATGA AGACAAAGAC AAGGATGAGA CGGAAACAGT TAAGCAGACT 
CAAACGTCTG AGGTTTATGA TGGTGACGGC CCCAAAAATG TGAGATCTGA TGTTTCTGAT 
CAAGAGGATG ATGAAGAAAG TGAAGAATGT CCAGTGTCTA TTAATTTGTC CAAAGCTGAA 
ACTCAGGCTT TGACTAATTA CGGAAGTGGA GAAGATGAGA ATGAAGATGA AGAAATAGAA 
GAATTTGAAG AGGGACCTGT AGATGTCCAG ACGTCACTTC AGGCTAACAC TGAAACTACA 
GAAGAAAATG AACACGATGA TCAGGTTCCA CAGCATGATT TTGAAAAATC ATCAGAAAGC 
AAAAATGTCC CATCAGAACA AGAACCCACT ACTAGTAAAG ACTTTCTTTT GATGACAGGT 
GACCAGAATA GCACTCCTGT GAAGCCCTGT TACCTCAGTA TCGTGGAAAA TGAGCAACCT 
TTAAATAGCC CTGTCCAGAA GGACACTCTC ACTACCATCG ACTCATCCAG TCAGCCTAAC 
CCTTTGCCTT TACCTTTAAC TGAAATTGAA ACCTTGGTGC CTAGAGTCAA AGAAGTGAAA 
TCTGCTCAGG AGACTCCTGA AAGTTCTTTG GCTGGAAGTC CTGATACTGA ATCTCCAGTG 
TTAGTGAATG ACTATGAAGC AGAATCTGGT AACATAAGTC AAAAATCTGA TGAAGAAGAC 
TTTGTGAAAG TTGAAGATTT ACCACTTAAA CTGACAATAT ATTCAGAGGC AGATCTAAGG 
AAGAAAATGG CCGAAGAAGA ACAGAAAAAC CACTTATCTG GTGAAATATG TGCAATGCAG 
ACTGAAGAAT TAGCTGGGAA TTCTCAGACA CTAAAAGAAC CTGAGCAAGT ATGTGCTCAG 
TCTGCCATCT TAAGATGGAA ACCCTTACCT GTGCCTTTTA AACTGTTGGT GGTGTCATCA 
GGGAATTATT GTAGCAAAAA ACAGTGGGAG CCCAAAGTAC ATGAGATGTC TCCAGAGGCT 
CAGCTAACTC TTTCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( XM_025279480.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025135265.1 Bubalus bubalis pericentriolar material 1 (PCM1), transcript variant X13, mRNA.

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CloneID	OBu42301
Clone ID Related Accession (Same CDS sequence)	XM_025279480.1
Accession Version	XM_025279480.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6480bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	pericentriolar material 1 protein isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037545.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG GAGGAGGTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
AGCAATGAGA GTGTTGATGA CCGACTCAAC AATATGGATT GGGGCAGCCA GCAGAAGAAG 
GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA 
ACTAACGATA TTTCTCCGGA GTCATCACCG GGAGTTGGAA GACGAAGAAC AAAGACTTCA 
CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TCCCCGAGCA GGCAGAACTA 
GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCTCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCCAC TGTAAGTCCT 
CAAAAGAGAG AAATGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAGATTCGTG AGTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGACCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTCACT 
CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT CCAGAAGATT 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCGGT CTGAGGCTTC AGATGCCACG 
GAGGCATCTT TTAGTCTGAG AATTCGGCCG TGCGTTGAGG ACAAACTAGG GAATTCAGCT 
TCACAAGCAC AGGTTTCAGA CACTGACGTT ACTACAAGTC CAAAAGGGAA AGGTGACAGA 
CCTCCTCAGC ATGACAGGGA GCTGAGGCCT AATAGGAAAT GTAGCCGAAA ACGTGGATTT 
CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATGGAAG AGATAGAAAA TTTGAAGAAA 
CAACATGATT TATTAAAAAG GATGTTACAG CAGCAGGAGC AGCTGAGAGC TCTACAAGGA 
CGACAAGCTG CTCTGCTTGC CCTGCAGCAT AAAGCTGAGC AGGCTATAGC TGTGATGGAT 
GATTCTGAAA AGGTTGCGGA GACAACTCCT GGCCATCATA CTGTACCAGG CAGACAGCCG 
GCTCATTCAC CTTTGCATCA GAGAGTACCC ATAAGAGAAA CTACAGGTAG CCTATCTGGA 
GTCAGTATCA CCTCTGAACT AAACGAAGAA TTGAATGATT TAATTCAGCA TTTTCATAAT 
CAGCTTCGTG ATTCTCAGCC TCCAGCTGTT CCAGACAATA GAAGACAAGC AGAAAGTCTT 
TCATTAACTA GGGAGATTTC TCAGAGCAGA AATCCTTCAG TTTCAGAGCA TTTACCTGAT 
GAGAAAGTCC AACTTTTTAG CAAGATGAGA GTGTTACAAG AAAAGAAACA AAAAATGGAC 
AAACTTCTTG GAGAACTTCA TACACTTCGA GATCAGCATT TGAACAATTC ATCATTTGTG 
CCTTCTTCAG CCTCTCCACA AAGGAGTGTG GATCAGAGAA GTACAACCAC AGCTCCTTCT 
GCTCCTGTAG TCTTAACACC AGTGGTCAAT GGAGAATCCA ATAACCTCAC GCCATCTGTT 
CCTTATCCTG CTGCTTCTCT AGTTTCTCAG AATCAGAATG AAAATGAAGG CCACCTAAAT 
CCAACTGAAA AACTCCAGAA GTTAAATGAA GTTCGAAAGA GGTTGAATGA ACTAAGAGAA 
TTAGTTCATT ATTATGAGCA AACATCAGAT ATGATGACAG ATGCTGTGAA TGAAAACACA 
AAAGAAGAAG AAACTGAAGA ATCAGAATAT GATTCGGAGC ATGAAAACTC GGAACCTGTT 
ACTAACATTC GAAATCCACA AGTGGCTGCC ACCTGGAACG AAGTAAATAG TAATTCTAAT 
GCACAGTGTG TTTCCAACAA TAGAGATGGG CGAGCAGTTA ATTCTAACTG TGAAATTAAC 
AACAGGTCTG CTGCCAACAT AAGGGCTCTA AACATGCCTC CTCTTTCAGA TTGTCGATAC 
AATAGAGAAG GAGAACAGGA GATGCCTGTT GCACAAGGTC ATGAGGAGGA GGAGGAGGAG 
GAGGAGGAAG CAGAAGATGA GGCCCTCAGT GGAGCTTCAC TGTCTAGTCA CAGCAGCAGC 
CTGATCATTG AGACTCCTGA AGATGCTGAA TTTGAACATA AGATCCAGAA GCTTATGGCT 
GCAAAACTGA AACTTAGACA CTTACAGAAC CTTGTAGCCA TGGTGCAGGA CGATGATGCA 
GCCAATCAAG GAGTTACTTC TGTGGAGGAT TTCTTCCCAG CAGAAGACAC CAAACAAAAT 
GCAAATAACA CTAGAGGAAA TGCCAATAAA ACACAGAAAG ATGCTGGAGT AAATGAAAAG 
GCAAGGGAGA AATTTTATGA GGCTAAACTA CAGCAGCAAC AGAGAGAACT CAAACAATTG 
CAGGAAGAAA GAAAGAAACT GATTGAGATT CAAGAGAAAA TTCAAGAATT GCAAAAGGCA 
TGTCCTAACC TACAGCTGTC AGCTGCTAGT GTGGGTAATT GTCCCACAAA AACACATATG 
CCAGCTGTTA CTTCATCCCC AACCGTTAAT GAAAATGAAG CCAGTACAAA CAAATCTGGT 
TTTGAGCCTC ATGATTCTTC AGTGGTAGAT AATGAGGTAT GGTCAGATAT GCGAAGACAT 
GAAATGTTAA GGGAGGAGCT ACGACAGAGA AGAAAGCAGC TGGAAGCTCT GATGGCTGAA 
CATCAGAGGA GGCAAGGTCT GGCTGAAACT GCATCTCCAG TGGCTGTTTC ACTGAGAAGT 
GATGGATCTG AGAACCTGTG TACACCTCAG CAAAGCAGAA CAGAAAAAAC AATGGCAACT 
TGGGGAGGTT CTACCCAGTG TGCCCTAGAT GAAGAAGGAG ATGAAGATGG TTACCTTTCT 
GAAGGAATTA TTCGGACAGA CGAAGAGGAG GAAGAGGAGG AGCAAGATGC CAGTTCCAAT 
GATAACTTTC ATATGTATCC TCCAAACAGT GCAAATCATA ATTCTTACAA TGTAAAGGAA 
ACTAAAAATA GGTGGAAGAA CAATCGCCCT TTTTCAGCAG ATGGAAATTA TCGTCCTTTA 
GCCAAGACAA GACAGCAGAA TATCAGCATG CAGCGTCAGG AAAACCTTCG CTGGGTGTCC 
GAACTCTCTT ATGTAGAAGA GAAAGAACAA TGGCAAGAAC AAATTAATCA GCTAAAGAAA 
CAACTTGATT TCAGTGTCAA TATTTGTCAG ACTTTAATGC AAGACCAGCA GACTCTGTCT 
TGTCTGCTAC AAACTCTTCT CACGAGTCCT TATAGTGTTA TGCCCAGCAG TGTTGCATCC 
CCGCAAGTGC ACCTTATAAT GCACCAGTTG AATCAGTGCT ATACCCAGCT AACGTGGCAG 
CAGAACAATG TCCAGAGGTT GAAACAAATG CTCAGTGAAC TCATGCGCCA ACAAAATCAG 
CATCCAGAAA AACCTGCAAG CAAGGAAGGA GGCAGTGGTA CATCACACCC TCCTTCTCCC 
AGTTTATTTT GTCCTTTCAA CTTTCCAACT CAGCCTGTAA ATCTGTTTAA CCTACCGGGG 
TTTACTAATT TTTCGTCATT TGCACCAAGT ATGAATTTCA GCCCTTTATT TCCTTCTAAT 
TTTGGAGATT TTTCTCAAAA TATTTCTACA CCCACTGAAC AGCAGCAACC GTTAGCCCAG 
AATCCTTCAG GGAAAACGGA ATACATGGCT TTTCCAAAAC CTTTTGAAAG TAGTTCTTCT 
ATTGGAGCGG AAAAACAAAG GAATCAAAAA CAGCCTGAAG AGGAGGTGGA GAACAGTAGG 
ACACCATGGT TTTATGACCA AGAAGGTGAA GTAGAAAAGC CATTTCTCAA GACTGGATTT 
GCAGTGTCTG TAGAGAAAAC AACAAATAGT AATCGCAAAA ATCAACTAGA TACCAGTAGG 
AGAAGACGCC AGTTTGATGA AGTGTCCTTA GAAAGCTTTA ACAGTATGCT TGACCCAGTA 
GATCCAACAA CAGTAACTAA AACATTCAAG TCAAGAAAAG CATCTGCACA GGCCAGCCTG 
GCATCTAAAG ATAAAACTCC CAAATCAAAG AGTAAGAAGA GGCATTCTAC TCAGCTGAAA 
AGCAGAGTGA AAAATATCGG GTATGAAAGT GCCAGTGTGT CTAGCACATG TGAACCTTGC 
AAAAGTAGAA ACAGACATTC AGCCCAAACT GAAGAGCCTG TTCAAGCAAA AGTACTCAGC 
AGAAAGAATC ATGAGCAGCT GGAAAAAGTC ATAAGATGTA GTAGGTCTAC AGAAATATCT 
TCAGAAACCG GGAGTGATTT TTCCATGTTC GAAGCTTTGC GGGATACCAT TTATTCTGAA 
GTAGCTACGT TGATTTCTCA AAATGAATCT CGTCCACATT TTCTTATTGA ACTCTTCCAT 
GAGCTTCAGC TACTTAATAC AGACTATTTG AGACAGAGGG CCTTATATGC ATTGCAGGAC 
ATAGTATCCA GGCATATTTC TGAGAACCAT GAAAAAGAAG GAGAAAATAT AAAGTCCGTG 
AATTCTGGTA CTTGGATAGC ATCCAACTCA GAACTTACTC CCAGTGAAAG CCTTGCTACT 
ACTGATGATG AAACTTTTGA GAAGAACTTT GAGAGAGAAA CACATAAAAT AAGTGAGCAA 
AATGATGCTG ATAATGCTAG TGTATCTTCA ACTTGTGAGC CTTTTGCAAC AGATGTTCTG 
GGCGACACTG TGATTCACTT AGATCAGGCA TTAGCCAGAA TGAGAGACTA TACGCATATG 
AAGATGGAGG CTGAAAGTAG TCCAGACGTC AGGTGCACCT GCAGGATCAT TGAGGATGAC 
GATGGTGCTG CTGCCACAAC CACGATTAGT AATAATTTAG AAGAAACTCC TGTTATTGAA 
AATCATAGTT CACAGCAACC CGTGAGTGAA GTTGCTACCG TCCCGTGTCC TAGAATTGAT 
ACTCAGCAAC TGGACCGGCA AATTAAAGCA ATTATGAAAG AAGTCATTCC TTTTTTGAAG 
GAGCACATGG ATGAAGTATG TTCTTCTCAA CTTCTAACCT CAGTAAGACG CATGGTTTTG 
GTCCTTACTC AGCAAAACAA TGAGAGCAAA GAGTTTGTAA AGTTCTTTCA TAAGCAGCTT 
GGAAGTATAT TACAGGATTC ACTCGCAAAA TTTGCTGGCA GAAAACTGAA GGATTGTGGA 
GAAGATCTTC TTGTAGAGAT ATCTGAAGTG TTATTTAATG AATTGGCTTT CTTTAAGCTC 
ATGCAAGATT TGGATAATAA TAGTGTAACT GTTAAACAGA GATGCAAAAG GAAAATAGAA 
GCTGCTGGAG TGATACAGTC TTATGCAAAA GAGGCCAAAA GGATTCTTGA AGGAGATCAC 
AGTTCACCTG CTGGAGAAAT TGATGATGAA GACAAAGACA AGGATGAGAC GGAAACAGTT 
AAGCAGACTC AAACGTCTGA GGTTTATGAT GGTGACGGCC CCAAAAATGT GAGATCTGAT 
GTTTCTGATC AAGAGGATGA TGAAGAAAGT GAAGAATGTC CAGTGTCTAT TAATTTGTCC 
AAAGCTGAAA CTCAGGCTTT GACTAATTAC GGAAGTGGAG AAGATGAGAA TGAAGATGAA 
GAAATAGAAG AATTTGAAGA GGGACCTGTA GATGTCCAGA CGTCACTTCA GGCTAACACT 
GAAACTACAG AAGAAAATGA ACACGATGAT CAGGTTCCAC AGCATGATTT TGAAAAATCA 
TCAGAAAGCA AAAATGTCCC ATCAGAACAA GAACCCACTA CTAGTAAAGG TGACCAGAAT 
AGCACTCCTG TGAAGCCCTG TTACCTCAGT ATCGTGGAAA ATGAGCAACC TTTAAATAGC 
CCTGTCCAGA AGGACACTCT CACTACCATC GACTCATCCA GTCAGCCTAA CCCTTTGCCT 
TTACCTTTAA CTGAAATTGA AACCTTGGTG CCTAGAGTCA AAGAAGTGAA ATCTGCTCAG 
GAGACTCCTG AAAGTTCTTT GGCTGGAAGT CCTGATACTG AATCTCCAGT GTTAGTGAAT 
GACTATGAAG CAGAATCTGG TAACATAAGT CAAAAATCTG ATGAAGAAGA CTTTGTGAAA 
GTTGAAGATT TACCACTTAA ACTGACAATA TATTCAGAGG CAGATCTAAG GAAGAAAATG 
GCCGAAGAAG AACAGAAAAA CCACTTATCT GGTGAAATAT GTGCAATGCA GACTGAAGAA 
TTAGCTGGGA ATTCTCAGAC ACTAAAAGAA CCTGAAACAG TGGGAGCCCA AAGTACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025135265.1
CDS	392..6871
Translation	MATGGGPFEEGVNDQDLPNWSNESVDDRLNNMDWGSQQKKANRSSEKNKRKFGVESDKRVTNDISPESSPGVGRRRTKTSLTFPHSRYMTQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDATVSPQKREMIGSAQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIREYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLQKILARENEEEDVRTIDSAVGSGSVAESTSLNIDVRSEASDATEASFSLRIRPCVEDKLGNSASQAQVSDTDVTTSPKGKGDRPPQHDRELRPNRKCSRKRGFPSKARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSEKVAETTPGHHTVPGRQPAHSPLHQRVPIRETTGSLSGVSITSELNEELNDLIQHFHNQLRDSQPPAVPDNRRQAESLSLTREISQSRNPSVSEHLPDEKVQLFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSFVPSSASPQRSVDQRSTTTAPSAPVVLTPVVNGESNNLTPSVPYPAASLVSQNQNENEGHLNPTEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENTKEEETEESEYDSEHENSEPVTNIRNPQVAATWNEVNSNSNAQCVSNNRDGRAVNSNCEINNRSAANIRALNMPPLSDCRYNREGEQEMPVAQGHEEEEEEEEEAEDEALSGASLSSHSSSLIIETPEDAEFEHKIQKLMAAKLKLRHLQNLVAMVQDDDAANQGVTSVEDFFPAEDTKQNANNTRGNANKTQKDAGVNEKAREKFYEAKLQQQQRELKQLQEERKKLIEIQEKIQELQKACPNLQLSAASVGNCPTKTHMPAVTSSPTVNENEASTNKSGFEPHDSSVVDNEVWSDMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETASPVAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGIIRTDEEEEEEEQDASSNDNFHMYPPNSANHNSYNVKETKNRWKNNRPFSADGNYRPLAKTRQQNISMQRQENLRWVSELSYVEEKEQWQEQINQLKKQLDFSVNICQTLMQDQQTLSCLLQTLLTSPYSVMPSSVASPQVHLIMHQLNQCYTQLTWQQNNVQRLKQMLSELMRQQNQHPEKPASKEGGSGTSHPPSPSLFCPFNFPTQPVNLFNLPGFTNFSSFAPSMNFSPLFPSNFGDFSQNISTPTEQQQPLAQNPSGKTEYMAFPKPFESSSSIGAEKQRNQKQPEEEVENSRTPWFYDQEGEVEKPFLKTGFAVSVEKTTNSNRKNQLDTSRRRRQFDEVSLESFNSMLDPVDPTTVTKTFKSRKASAQASLASKDKTPKSKSKKRHSTQLKSRVKNIGYESASVSSTCEPCKSRNRHSAQTEEPVQAKVLSRKNHEQLEKVIRCSRSTEISSETGSDFSMFEALRDTIYSEVATLISQNESRPHFLIELFHELQLLNTDYLRQRALYALQDIVSRHISENHEKEGENIKSVNSGTWIASNSELTPSESLATTDDETFEKNFERETHKISEQNDADNASVSSTCEPFATDVLGDTVIHLDQALARMRDYTHMKMEAESSPDVRCTCRIIEDDDGAAATTTISNNLEETPVIENHSSQQPVSEVATVPCPRIDTQQLDRQIKAIMKEVIPFLKEHMDEVCSSQLLTSVRRMVLVLTQQNNESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSVTVKQRCKRKIEAAGVIQSYAKEAKRILEGDHSSPAGEIDDEDKDKDETETVKQTQTSEVYDGDGPKNVRSDVSDQEDDEESEECPVSINLSKAETQALTNYGSGEDENEDEEIEEFEEGPVDVQTSLQANTETTEENEHDDQVPQHDFEKSSESKNVPSEQEPTTSKGDQNSTPVKPCYLSIVENEQPLNSPVQKDTLTTIDSSSQPNPLPLPLTEIETLVPRVKEVKSAQETPESSLAGSPDTESPVLVNDYEAESGNISQKSDEEDFVKVEDLPLKLTIYSEADLRKKMAEEEQKNHLSGEICAMQTEELAGNSQTLKEPETVGAQST

Target ORF information:

RefSeq Version	XM_025279480.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis pericentriolar material 1 (PCM1), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025279480.1

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
AGCAATGAGA GTGTTGATGA CCGACTCAAC AATATGGATT GGGGCAGCCA GCAGAAGAAG 
GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA 
ACTAACGATA TTTCTCCGGA GTCATCACCG GGAGTTGGAA GACGAAGAAC AAAGACTTCA 
CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TCCCCGAGCA GGCAGAACTA 
GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCTCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCCAC TGTAAGTCCT 
CAAAAGAGAG AAATGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAGATTCGTG AGTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGACCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTCACT 
CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT CCAGAAGATT 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCGGT CTGAGGCTTC AGATGCCACG 
GAGGCATCTT TTAGTCTGAG AATTCGGCCG TGCGTTGAGG ACAAACTAGG GAATTCAGCT 
TCACAAGCAC AGGTTTCAGA CACTGACGTT ACTACAAGTC CAAAAGGGAA AGGTGACAGA 
CCTCCTCAGC ATGACAGGGA GCTGAGGCCT AATAGGAAAT GTAGCCGAAA ACGTGGATTT 
CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATGGAAG AGATAGAAAA TTTGAAGAAA 
CAACATGATT TATTAAAAAG GATGTTACAG CAGCAGGAGC AGCTGAGAGC TCTACAAGGA 
CGACAAGCTG CTCTGCTTGC CCTGCAGCAT AAAGCTGAGC AGGCTATAGC TGTGATGGAT 
GATTCTGAAA AGGTTGCGGA GACAACTCCT GGCCATCATA CTGTACCAGG CAGACAGCCG 
GCTCATTCAC CTTTGCATCA GAGAGTACCC ATAAGAGAAA CTACAGGTAG CCTATCTGGA 
GTCAGTATCA CCTCTGAACT AAACGAAGAA TTGAATGATT TAATTCAGCA TTTTCATAAT 
CAGCTTCGTG ATTCTCAGCC TCCAGCTGTT CCAGACAATA GAAGACAAGC AGAAAGTCTT 
TCATTAACTA GGGAGATTTC TCAGAGCAGA AATCCTTCAG TTTCAGAGCA TTTACCTGAT 
GAGAAAGTCC AACTTTTTAG CAAGATGAGA GTGTTACAAG AAAAGAAACA AAAAATGGAC 
AAACTTCTTG GAGAACTTCA TACACTTCGA GATCAGCATT TGAACAATTC ATCATTTGTG 
CCTTCTTCAG CCTCTCCACA AAGGAGTGTG GATCAGAGAA GTACAACCAC AGCTCCTTCT 
GCTCCTGTAG TCTTAACACC AGTGGTCAAT GGAGAATCCA ATAACCTCAC GCCATCTGTT 
CCTTATCCTG CTGCTTCTCT AGTTTCTCAG AATCAGAATG AAAATGAAGG CCACCTAAAT 
CCAACTGAAA AACTCCAGAA GTTAAATGAA GTTCGAAAGA GGTTGAATGA ACTAAGAGAA 
TTAGTTCATT ATTATGAGCA AACATCAGAT ATGATGACAG ATGCTGTGAA TGAAAACACA 
AAAGAAGAAG AAACTGAAGA ATCAGAATAT GATTCGGAGC ATGAAAACTC GGAACCTGTT 
ACTAACATTC GAAATCCACA AGTGGCTGCC ACCTGGAACG AAGTAAATAG TAATTCTAAT 
GCACAGTGTG TTTCCAACAA TAGAGATGGG CGAGCAGTTA ATTCTAACTG TGAAATTAAC 
AACAGGTCTG CTGCCAACAT AAGGGCTCTA AACATGCCTC CTCTTTCAGA TTGTCGATAC 
AATAGAGAAG GAGAACAGGA GATGCCTGTT GCACAAGGTC ATGAGGAGGA GGAGGAGGAG 
GAGGAGGAAG CAGAAGATGA GGCCCTCAGT GGAGCTTCAC TGTCTAGTCA CAGCAGCAGC 
CTGATCATTG AGACTCCTGA AGATGCTGAA TTTGAACATA AGATCCAGAA GCTTATGGCT 
GCAAAACTGA AACTTAGACA CTTACAGAAC CTTGTAGCCA TGGTGCAGGA CGATGATGCA 
GCCAATCAAG GAGTTACTTC TGTGGAGGAT TTCTTCCCAG CAGAAGACAC CAAACAAAAT 
GCAAATAACA CTAGAGGAAA TGCCAATAAA ACACAGAAAG ATGCTGGAGT AAATGAAAAG 
GCAAGGGAGA AATTTTATGA GGCTAAACTA CAGCAGCAAC AGAGAGAACT CAAACAATTG 
CAGGAAGAAA GAAAGAAACT GATTGAGATT CAAGAGAAAA TTCAAGAATT GCAAAAGGCA 
TGTCCTAACC TACAGCTGTC AGCTGCTAGT GTGGGTAATT GTCCCACAAA AACACATATG 
CCAGCTGTTA CTTCATCCCC AACCGTTAAT GAAAATGAAG CCAGTACAAA CAAATCTGGT 
TTTGAGCCTC ATGATTCTTC AGTGGTAGAT AATGAGGTAT GGTCAGATAT GCGAAGACAT 
GAAATGTTAA GGGAGGAGCT ACGACAGAGA AGAAAGCAGC TGGAAGCTCT GATGGCTGAA 
CATCAGAGGA GGCAAGGTCT GGCTGAAACT GCATCTCCAG TGGCTGTTTC ACTGAGAAGT 
GATGGATCTG AGAACCTGTG TACACCTCAG CAAAGCAGAA CAGAAAAAAC AATGGCAACT 
TGGGGAGGTT CTACCCAGTG TGCCCTAGAT GAAGAAGGAG ATGAAGATGG TTACCTTTCT 
GAAGGAATTA TTCGGACAGA CGAAGAGGAG GAAGAGGAGG AGCAAGATGC CAGTTCCAAT 
GATAACTTTC ATATGTATCC TCCAAACAGT GCAAATCATA ATTCTTACAA TGTAAAGGAA 
ACTAAAAATA GGTGGAAGAA CAATCGCCCT TTTTCAGCAG ATGGAAATTA TCGTCCTTTA 
GCCAAGACAA GACAGCAGAA TATCAGCATG CAGCGTCAGG AAAACCTTCG CTGGGTGTCC 
GAACTCTCTT ATGTAGAAGA GAAAGAACAA TGGCAAGAAC AAATTAATCA GCTAAAGAAA 
CAACTTGATT TCAGTGTCAA TATTTGTCAG ACTTTAATGC AAGACCAGCA GACTCTGTCT 
TGTCTGCTAC AAACTCTTCT CACGAGTCCT TATAGTGTTA TGCCCAGCAG TGTTGCATCC 
CCGCAAGTGC ACCTTATAAT GCACCAGTTG AATCAGTGCT ATACCCAGCT AACGTGGCAG 
CAGAACAATG TCCAGAGGTT GAAACAAATG CTCAGTGAAC TCATGCGCCA ACAAAATCAG 
CATCCAGAAA AACCTGCAAG CAAGGAAGGA GGCAGTGGTA CATCACACCC TCCTTCTCCC 
AGTTTATTTT GTCCTTTCAA CTTTCCAACT CAGCCTGTAA ATCTGTTTAA CCTACCGGGG 
TTTACTAATT TTTCGTCATT TGCACCAAGT ATGAATTTCA GCCCTTTATT TCCTTCTAAT 
TTTGGAGATT TTTCTCAAAA TATTTCTACA CCCACTGAAC AGCAGCAACC GTTAGCCCAG 
AATCCTTCAG GGAAAACGGA ATACATGGCT TTTCCAAAAC CTTTTGAAAG TAGTTCTTCT 
ATTGGAGCGG AAAAACAAAG GAATCAAAAA CAGCCTGAAG AGGAGGTGGA GAACAGTAGG 
ACACCATGGT TTTATGACCA AGAAGGTGAA GTAGAAAAGC CATTTCTCAA GACTGGATTT 
GCAGTGTCTG TAGAGAAAAC AACAAATAGT AATCGCAAAA ATCAACTAGA TACCAGTAGG 
AGAAGACGCC AGTTTGATGA AGTGTCCTTA GAAAGCTTTA ACAGTATGCT TGACCCAGTA 
GATCCAACAA CAGTAACTAA AACATTCAAG TCAAGAAAAG CATCTGCACA GGCCAGCCTG 
GCATCTAAAG ATAAAACTCC CAAATCAAAG AGTAAGAAGA GGCATTCTAC TCAGCTGAAA 
AGCAGAGTGA AAAATATCGG GTATGAAAGT GCCAGTGTGT CTAGCACATG TGAACCTTGC 
AAAAGTAGAA ACAGACATTC AGCCCAAACT GAAGAGCCTG TTCAAGCAAA AGTACTCAGC 
AGAAAGAATC ATGAGCAGCT GGAAAAAGTC ATAAGATGTA GTAGGTCTAC AGAAATATCT 
TCAGAAACCG GGAGTGATTT TTCCATGTTC GAAGCTTTGC GGGATACCAT TTATTCTGAA 
GTAGCTACGT TGATTTCTCA AAATGAATCT CGTCCACATT TTCTTATTGA ACTCTTCCAT 
GAGCTTCAGC TACTTAATAC AGACTATTTG AGACAGAGGG CCTTATATGC ATTGCAGGAC 
ATAGTATCCA GGCATATTTC TGAGAACCAT GAAAAAGAAG GAGAAAATAT AAAGTCCGTG 
AATTCTGGTA CTTGGATAGC ATCCAACTCA GAACTTACTC CCAGTGAAAG CCTTGCTACT 
ACTGATGATG AAACTTTTGA GAAGAACTTT GAGAGAGAAA CACATAAAAT AAGTGAGCAA 
AATGATGCTG ATAATGCTAG TGTATCTTCA ACTTGTGAGC CTTTTGCAAC AGATGTTCTG 
GGCGACACTG TGATTCACTT AGATCAGGCA TTAGCCAGAA TGAGAGACTA TACGCATATG 
AAGATGGAGG CTGAAAGTAG TCCAGACGTC AGGTGCACCT GCAGGATCAT TGAGGATGAC 
GATGGTGCTG CTGCCACAAC CACGATTAGT AATAATTTAG AAGAAACTCC TGTTATTGAA 
AATCATAGTT CACAGCAACC CGTGAGTGAA GTTGCTACCG TCCCGTGTCC TAGAATTGAT 
ACTCAGCAAC TGGACCGGCA AATTAAAGCA ATTATGAAAG AAGTCATTCC TTTTTTGAAG 
GAGCACATGG ATGAAGTATG TTCTTCTCAA CTTCTAACCT CAGTAAGACG CATGGTTTTG 
GTCCTTACTC AGCAAAACAA TGAGAGCAAA GAGTTTGTAA AGTTCTTTCA TAAGCAGCTT 
GGAAGTATAT TACAGGATTC ACTCGCAAAA TTTGCTGGCA GAAAACTGAA GGATTGTGGA 
GAAGATCTTC TTGTAGAGAT ATCTGAAGTG TTATTTAATG AATTGGCTTT CTTTAAGCTC 
ATGCAAGATT TGGATAATAA TAGTGTAACT GTTAAACAGA GATGCAAAAG GAAAATAGAA 
GCTGCTGGAG TGATACAGTC TTATGCAAAA GAGGCCAAAA GGATTCTTGA AGGAGATCAC 
AGTTCACCTG CTGGAGAAAT TGATGATGAA GACAAAGACA AGGATGAGAC GGAAACAGTT 
AAGCAGACTC AAACGTCTGA GGTTTATGAT GGTGACGGCC CCAAAAATGT GAGATCTGAT 
GTTTCTGATC AAGAGGATGA TGAAGAAAGT GAAGAATGTC CAGTGTCTAT TAATTTGTCC 
AAAGCTGAAA CTCAGGCTTT GACTAATTAC GGAAGTGGAG AAGATGAGAA TGAAGATGAA 
GAAATAGAAG AATTTGAAGA GGGACCTGTA GATGTCCAGA CGTCACTTCA GGCTAACACT 
GAAACTACAG AAGAAAATGA ACACGATGAT CAGGTTCCAC AGCATGATTT TGAAAAATCA 
TCAGAAAGCA AAAATGTCCC ATCAGAACAA GAACCCACTA CTAGTAAAGG TGACCAGAAT 
AGCACTCCTG TGAAGCCCTG TTACCTCAGT ATCGTGGAAA ATGAGCAACC TTTAAATAGC 
CCTGTCCAGA AGGACACTCT CACTACCATC GACTCATCCA GTCAGCCTAA CCCTTTGCCT 
TTACCTTTAA CTGAAATTGA AACCTTGGTG CCTAGAGTCA AAGAAGTGAA ATCTGCTCAG 
GAGACTCCTG AAAGTTCTTT GGCTGGAAGT CCTGATACTG AATCTCCAGT GTTAGTGAAT 
GACTATGAAG CAGAATCTGG TAACATAAGT CAAAAATCTG ATGAAGAAGA CTTTGTGAAA 
GTTGAAGATT TACCACTTAA ACTGACAATA TATTCAGAGG CAGATCTAAG GAAGAAAATG 
GCCGAAGAAG AACAGAAAAA CCACTTATCT GGTGAAATAT GTGCAATGCA GACTGAAGAA 
TTAGCTGGGA ATTCTCAGAC ACTAAAAGAA CCTGAAACAG TGGGAGCCCA AAGTACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( XM_025279484.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025135269.1 Bubalus bubalis pericentriolar material 1 (PCM1), transcript variant X14, mRNA.

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CloneID	OBu42302
Clone ID Related Accession (Same CDS sequence)	XM_025279484.1
Accession Version	XM_025279484.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6465bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	pericentriolar material 1 protein isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037545.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG GAGGAGGTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
AGCAATGAGA GTGTTGATGA CCGACTCAAC AATATGGATT GGGGCAGCCA GCAGAAGAAG 
GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA 
ACTAACGATA TTTCTCCGGA GTCATCACCG GGAGTTGGAA GACGAAGAAC AAAGACTTCA 
CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TCCCCGAGCA GGCAGAACTA 
GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCTCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCCAC TGTAAGTCCT 
CAAAAGAGAG AAATGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAGATTCGTG AGTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGACCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTCACT 
CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT CCAGAAGATT 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCGGT CTGAGGCTTC AGATGCCACG 
GAGGCATCTT TTAGTCTGAG AATTCGGCCG TGCGTTGAGG ACAAACTAGG GAATTCAGCT 
TCACAAGCAC AGGTTTCAGA CACTGACGTT ACTACAAGTC CAAAAGGGAA AGGTGACAGA 
CCTCCTCAGC ATGACAGGGA GCTGAGGCCT AATAGGAAAT GTAGCCGAAA ACGTGGATTT 
CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATGGAAG AGATAGAAAA TTTGAAGAAA 
CAACATGATT TATTAAAAAG GATGTTACAG CAGCAGGAGC AGCTGAGAGC TCTACAAGGA 
CGACAAGCTG CTCTGCTTGC CCTGCAGCAT AAAGCTGAGC AGGCTATAGC TGTGATGGAT 
GATTCTGAAA AGGTTGCGGA GACAACTCCT GGCCATCATA CTGTACCAGG CAGACAGCCG 
GCTCATTCAC CTTTGCATCA GAGAGTACCC ATAAGAGAAA CTACAGGTAG CCTATCTGGA 
GTCAGTATCA CCTCTGAACT AAACGAAGAA TTGAATGATT TAATTCAGCA TTTTCATAAT 
CAGCTTCGTG ATTCTCAGCC TCCAGCTGTT CCAGACAATA GAAGACAAGC AGAAAGTCTT 
TCATTAACTA GGGAGATTTC TCAGAGCAGA AATCCTTCAG TTTCAGAGCA TTTACCTGAT 
GAGAAAGTCC AACTTTTTAG CAAGATGAGA GTGTTACAAG AAAAGAAACA AAAAATGGAC 
AAACTTCTTG GAGAACTTCA TACACTTCGA GATCAGCATT TGAACAATTC ATCATTTGTG 
CCTTCTTCAG CCTCTCCACA AAGGAGTGTG GATCAGAGAA GTACAACCAC AGCTCCTTCT 
GCTCCTGTAG TCTTAACACC AGTGGTCAAT GGAGAATCCA ATAACCTCAC GCCATCTGTT 
CCTTATCCTG CTGCTTCTCT AGTTTCTCAG AATCAGAATG AAAATGAAGG CCACCTAAAT 
CCAACTGAAA AACTCCAGAA GTTAAATGAA GTTCGAAAGA GGTTGAATGA ACTAAGAGAA 
TTAGTTCATT ATTATGAGCA AACATCAGAT ATGATGACAG ATGCTGTGAA TGAAAACACA 
AAAGAAGAAG AAACTGAAGA ATCAGAATAT GATTCGGAGC ATGAAAACTC GGAACCTGTT 
ACTAACATTC GAAATCCACA AGTGGCTGCC ACCTGGAACG AAGTAAATAG TAATTCTAAT 
GCACAGTGTG TTTCCAACAA TAGAGATGGG CGAGCAGTTA ATTCTAACTG TGAAATTAAC 
AACAGGTCTG CTGCCAACAT AAGGGCTCTA AACATGCCTC CTCTTTCAGC AGATTGTCGA 
TACAATAGAG AAGGAGAACA GGAGATGCCT GTTGCACAAG GTCATGAGGA GGAGGAGGAG 
GAGGAGGAGG AAGCAGAAGA TGAGGCCCTC AGTGGAGCTT CACTGTCTAG TCACAGCAGC 
AGCCTGATCA TTGAGACTCC TGAAGATGCT GAATTTGAAC ATAAGATCCA GAAGCTTATG 
GCTGCAAAAC TGAAACTTAG ACACTTACAG AACCTTGTAG CCATGGTGCA GGACGATGAT 
GCAGCCAATC AAGGAGTTAC TTCTGTGGAG GATTTCTTCC CAGCAGAAGA CACCAAACAA 
AATGCAAATA ACACTAGAGG AAATGCCAAT AAAACACAGA AAGATGCTGG AGTAAATGAA 
AAGGCAAGGG AGAAATTTTA TGAGGCTAAA CTACAGCAGC AACAGAGAGA ACTCAAACAA 
TTGCAGGAAG AAAGAAAGAA ACTGATTGAG ATTCAAGAGA AAATTCAAGA ATTGCAAAAG 
GCATGTCCTA ACCTACAGCT GTCAGCTGCT AGTGTGGGTA ATTGTCCCAC AAAAACACAT 
ATGCCAGCTG TTACTTCATC CCCAACCGTT AATGAAAATG AAGCCAGTAC AAACAAATCT 
GGTTTTGAGC CTCATGATTC TTCAGTGGTA GATAATGAGG TATGGTCAGA TATGCGAAGA 
CATGAAATGT TAAGGGAGGA GCTACGACAG AGAAGAAAGC AGCTGGAAGC TCTGATGGCT 
GAACATCAGA GGAGGCAAGG TCTGGCTGAA ACTGCATCTC CAGTGGCTGT TTCACTGAGA 
AGTGATGGAT CTGAGAACCT GTGTACACCT CAGCAAAGCA GAACAGAAAA AACAATGGCA 
ACTTGGGGAG GTTCTACCCA GTGTGCCCTA GATGAAGAAG GAGATGAAGA TGGTTACCTT 
TCTGAAGGAA TTATTCGGAC AGACGAAGAG GAGGAAGAGG AGGAGCAAGA TGCCAGTTCC 
AATGATAACT TTCATATGTA TCCTCCAAAC AGTGCAAATC ATAATTCTTA CAATGTAAAG 
GAAACTAAAA ATAGGTGGAA GAACAATCGC CCTTTTTCAG CAGATGGAAA TTATCGTCCT 
TTAGCCAAGA CAAGACAGCA GAATATCAGC ATGCAGCGTC AGGAAAACCT TCGCTGGGTG 
TCCGAACTCT CTTATGTAGA AGAGAAAGAA CAATGGCAAG AACAAATTAA TCAGCTAAAG 
AAACAACTTG ATTTCAGTGT CAATATTTGT CAGACTTTAA TGCAAGACCA GCAGACTCTG 
TCTTGTCTGC TACAAACTCT TCTCACGAGT CCTTATAGTG TTATGCCCAG CAGTGTTGCA 
TCCCCGCAAG TGCACCTTAT AATGCACCAG TTGAATCAGT GCTATACCCA GCTAACGTGG 
CAGCAGAACA ATGTCCAGAG GTTGAAACAA ATGCTCAGTG AACTCATGCG CCAACAAAAT 
CAGCATCCAG AAAAACCTGC AAGCAAGGAA GGAGGCAGTG GTACATCACA CCCTCCTTCT 
CCCAGTTTAT TTTGTCCTTT CAACTTTCCA ACTCAGCCTG TAAATCTGTT TAACCTACCG 
GGGTTTACTA ATTTTTCGTC ATTTGCACCA AGTATGAATT TCAGCCCTTT ATTTCCTTCT 
AATTTTGGAG ATTTTTCTCA AAATATTTCT ACACCCACTG AACAGCAGCA ACCGTTAGCC 
CAGAATCCTT CAGGGAAAAC GGAATACATG GCTTTTCCAA AACCTTTTGA AAGTAGTTCT 
TCTATTGGAG CGGAAAAACA AAGGAATCAA AAACAGCCTG AAGAGGAGGT GGAGAACAGT 
AGGACACCAT GGTTTTATGA CCAAGAAGGT GAAGTAGAAA AGCCATTTCT CAAGACTGGA 
TTTGCAGTGT CTGTAGAGAA AACAACAAAT AGTAATCGCA AAAATCAACT AGATACCAGT 
AGGAGAAGAC GCCAGTTTGA TGAAGTGTCC TTAGAAAGCT TTAACAGTAT GCTTGACCCA 
GTAGATCCAA CAACAGTAAC TAAAACATTC AAGTCAAGAA AAGCATCTGC ACAGGCCAGC 
CTGGCATCTA AAGATAAAAC TCCCAAATCA AAGAGTAAGA AGAGGCATTC TACTCAGCTG 
AAAAGCAGAG TGAAAAATAT CGAAACCGGG AGTGATTTTT CCATGTTCGA AGCTTTGCGG 
GATACCATTT ATTCTGAAGT AGCTACGTTG ATTTCTCAAA ATGAATCTCG TCCACATTTT 
CTTATTGAAC TCTTCCATGA GCTTCAGCTA CTTAATACAG ACTATTTGAG ACAGAGGGCC 
TTATATGCAT TGCAGGACAT AGTATCCAGG CATATTTCTG AGAACCATGA AAAAGAAGGA 
GAAAATATAA AGTCCGTGAA TTCTGGTACT TGGATAGCAT CCAACTCAGA ACTTACTCCC 
AGTGAAAGCC TTGCTACTAC TGATGATGAA ACTTTTGAGA AGAACTTTGA GAGAGAAACA 
CATAAAATAA GTGAGCAAAA TGATGCTGAT AATGCTAGTG TATCTTCAAC TTGTGAGCCT 
TTTGCAACAG ATGTTCTGGG CGACACTGTG ATTCACTTAG ATCAGGCATT AGCCAGAATG 
AGAGACTATA CGCATATGAA GATGGAGGCT GAAAGTAGTC CAGACGTCAG GTGCACCTGC 
AGGATCATTG AGGATGACGA TGGTGCTGCT GCCACAACCA CGATTAGTAA TAATTTAGAA 
GAAACTCCTG TTATTGAAAA TCATAGTTCA CAGCAACCCG TGAGTGAAGT TGCTACCGTC 
CCGTGTCCTA GAATTGATAC TCAGCAACTG GACCGGCAAA TTAAAGCAAT TATGAAAGAA 
GTCATTCCTT TTTTGAAGGA GCACATGGAT GAAGTATGTT CTTCTCAACT TCTAACCTCA 
GTAAGACGCA TGGTTTTGGT CCTTACTCAG CAAAACAATG AGAGCAAAGA GTTTGTAAAG 
TTCTTTCATA AGCAGCTTGG AAGTATATTA CAGGATTCAC TCGCAAAATT TGCTGGCAGA 
AAACTGAAGG ATTGTGGAGA AGATCTTCTT GTAGAGATAT CTGAAGTGTT ATTTAATGAA 
TTGGCTTTCT TTAAGCTCAT GCAAGATTTG GATAATAATA GTGTAACTGT TAAACAGAGA 
TGCAAAAGGA AAATAGAAGC TGCTGGAGTG ATACAGTCTT ATGCAAAAGA GGCCAAAAGG 
ATTCTTGAAG GAGATCACAG TTCACCTGCT GGAGAAATTG ATGATGAAGA CAAAGACAAG 
GATGAGACGG AAACAGTTAA GCAGACTCAA ACGTCTGAGG TTTATGATGG TGACGGCCCC 
AAAAATGTGA GATCTGATGT TTCTGATCAA GAGGATGATG AAGAAAGTGA AGAATGTCCA 
GTGTCTATTA ATTTGTCCAA AGCTGAAACT CAGGCTTTGA CTAATTACGG AAGTGGAGAA 
GATGAGAATG AAGATGAAGA AATAGAAGAA TTTGAAGAGG GACCTGTAGA TGTCCAGACG 
TCACTTCAGG CTAACACTGA AACTACAGAA GAAAATGAAC ACGATGATCA GGTTCCACAG 
CATGATTTTG AAAAATCATC AGAAAGCAAA AATGTCCCAT CAGAACAAGA ACCCACTACT 
AGTAAAGACT TTCTTTTGAT GACAGGTGAC CAGAATAGCA CTCCTGTGAA GCCCTGTTAC 
CTCAGTATCG TGGAAAATGA GCAACCTTTA AATAGCCCTG TCCAGAAGGA CACTCTCACT 
ACCATCGACT CATCCAGTCA GCCTAACCCT TTGCCTTTAC CTTTAACTGA AATTGAAACC 
TTGGTGCCTA GAGTCAAAGA AGTGAAATCT GCTCAGGAGA CTCCTGAAAG TTCTTTGGCT 
GGAAGTCCTG ATACTGAATC TCCAGTGTTA GTGAATGACT ATGAAGCAGA ATCTGGTAAC 
ATAAGTCAAA AATCTGATGA AGAAGACTTT GTGAAAGTTG AAGATTTACC ACTTAAACTG 
ACAATATATT CAGAGGCAGA TCTAAGGAAG AAAATGGCCG AAGAAGAACA GAAAAACCAC 
TTATCTGGTG AAATATGTGC AATGCAGACT GAAGAATTAG CTGGGAATTC TCAGACACTA 
AAAGAACCTG AGCAAGTATG TGCTCAGTCT GCCATCTTAA GATGGAAACC CTTACCTGTG 
CCTTTTAAAC TGTTGGTGGT GTCATCAGGG AATTATTGTA GCAAAAAACA GTGGGAGCCC 
AAAGTACATG AGATGTCTCC AGAGGCTCAG CTAACTCTTT CTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025135269.1
CDS	392..6856
Translation	MATGGGPFEEGVNDQDLPNWSNESVDDRLNNMDWGSQQKKANRSSEKNKRKFGVESDKRVTNDISPESSPGVGRRRTKTSLTFPHSRYMTQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDATVSPQKREMIGSAQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIREYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLQKILARENEEEDVRTIDSAVGSGSVAESTSLNIDVRSEASDATEASFSLRIRPCVEDKLGNSASQAQVSDTDVTTSPKGKGDRPPQHDRELRPNRKCSRKRGFPSKARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSEKVAETTPGHHTVPGRQPAHSPLHQRVPIRETTGSLSGVSITSELNEELNDLIQHFHNQLRDSQPPAVPDNRRQAESLSLTREISQSRNPSVSEHLPDEKVQLFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSFVPSSASPQRSVDQRSTTTAPSAPVVLTPVVNGESNNLTPSVPYPAASLVSQNQNENEGHLNPTEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENTKEEETEESEYDSEHENSEPVTNIRNPQVAATWNEVNSNSNAQCVSNNRDGRAVNSNCEINNRSAANIRALNMPPLSADCRYNREGEQEMPVAQGHEEEEEEEEEAEDEALSGASLSSHSSSLIIETPEDAEFEHKIQKLMAAKLKLRHLQNLVAMVQDDDAANQGVTSVEDFFPAEDTKQNANNTRGNANKTQKDAGVNEKAREKFYEAKLQQQQRELKQLQEERKKLIEIQEKIQELQKACPNLQLSAASVGNCPTKTHMPAVTSSPTVNENEASTNKSGFEPHDSSVVDNEVWSDMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETASPVAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGIIRTDEEEEEEEQDASSNDNFHMYPPNSANHNSYNVKETKNRWKNNRPFSADGNYRPLAKTRQQNISMQRQENLRWVSELSYVEEKEQWQEQINQLKKQLDFSVNICQTLMQDQQTLSCLLQTLLTSPYSVMPSSVASPQVHLIMHQLNQCYTQLTWQQNNVQRLKQMLSELMRQQNQHPEKPASKEGGSGTSHPPSPSLFCPFNFPTQPVNLFNLPGFTNFSSFAPSMNFSPLFPSNFGDFSQNISTPTEQQQPLAQNPSGKTEYMAFPKPFESSSSIGAEKQRNQKQPEEEVENSRTPWFYDQEGEVEKPFLKTGFAVSVEKTTNSNRKNQLDTSRRRRQFDEVSLESFNSMLDPVDPTTVTKTFKSRKASAQASLASKDKTPKSKSKKRHSTQLKSRVKNIETGSDFSMFEALRDTIYSEVATLISQNESRPHFLIELFHELQLLNTDYLRQRALYALQDIVSRHISENHEKEGENIKSVNSGTWIASNSELTPSESLATTDDETFEKNFERETHKISEQNDADNASVSSTCEPFATDVLGDTVIHLDQALARMRDYTHMKMEAESSPDVRCTCRIIEDDDGAAATTTISNNLEETPVIENHSSQQPVSEVATVPCPRIDTQQLDRQIKAIMKEVIPFLKEHMDEVCSSQLLTSVRRMVLVLTQQNNESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSVTVKQRCKRKIEAAGVIQSYAKEAKRILEGDHSSPAGEIDDEDKDKDETETVKQTQTSEVYDGDGPKNVRSDVSDQEDDEESEECPVSINLSKAETQALTNYGSGEDENEDEEIEEFEEGPVDVQTSLQANTETTEENEHDDQVPQHDFEKSSESKNVPSEQEPTTSKDFLLMTGDQNSTPVKPCYLSIVENEQPLNSPVQKDTLTTIDSSSQPNPLPLPLTEIETLVPRVKEVKSAQETPESSLAGSPDTESPVLVNDYEAESGNISQKSDEEDFVKVEDLPLKLTIYSEADLRKKMAEEEQKNHLSGEICAMQTEELAGNSQTLKEPEQVCAQSAILRWKPLPVPFKLLVVSSGNYCSKKQWEPKVHEMSPEAQLTLS

Target ORF information:

RefSeq Version	XM_025279484.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis pericentriolar material 1 (PCM1), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025279484.1

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
AGCAATGAGA GTGTTGATGA CCGACTCAAC AATATGGATT GGGGCAGCCA GCAGAAGAAG 
GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA 
ACTAACGATA TTTCTCCGGA GTCATCACCG GGAGTTGGAA GACGAAGAAC AAAGACTTCA 
CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TCCCCGAGCA GGCAGAACTA 
GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCTCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCCAC TGTAAGTCCT 
CAAAAGAGAG AAATGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAGATTCGTG AGTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGACCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTCACT 
CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT CCAGAAGATT 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCGGT CTGAGGCTTC AGATGCCACG 
GAGGCATCTT TTAGTCTGAG AATTCGGCCG TGCGTTGAGG ACAAACTAGG GAATTCAGCT 
TCACAAGCAC AGGTTTCAGA CACTGACGTT ACTACAAGTC CAAAAGGGAA AGGTGACAGA 
CCTCCTCAGC ATGACAGGGA GCTGAGGCCT AATAGGAAAT GTAGCCGAAA ACGTGGATTT 
CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATGGAAG AGATAGAAAA TTTGAAGAAA 
CAACATGATT TATTAAAAAG GATGTTACAG CAGCAGGAGC AGCTGAGAGC TCTACAAGGA 
CGACAAGCTG CTCTGCTTGC CCTGCAGCAT AAAGCTGAGC AGGCTATAGC TGTGATGGAT 
GATTCTGAAA AGGTTGCGGA GACAACTCCT GGCCATCATA CTGTACCAGG CAGACAGCCG 
GCTCATTCAC CTTTGCATCA GAGAGTACCC ATAAGAGAAA CTACAGGTAG CCTATCTGGA 
GTCAGTATCA CCTCTGAACT AAACGAAGAA TTGAATGATT TAATTCAGCA TTTTCATAAT 
CAGCTTCGTG ATTCTCAGCC TCCAGCTGTT CCAGACAATA GAAGACAAGC AGAAAGTCTT 
TCATTAACTA GGGAGATTTC TCAGAGCAGA AATCCTTCAG TTTCAGAGCA TTTACCTGAT 
GAGAAAGTCC AACTTTTTAG CAAGATGAGA GTGTTACAAG AAAAGAAACA AAAAATGGAC 
AAACTTCTTG GAGAACTTCA TACACTTCGA GATCAGCATT TGAACAATTC ATCATTTGTG 
CCTTCTTCAG CCTCTCCACA AAGGAGTGTG GATCAGAGAA GTACAACCAC AGCTCCTTCT 
GCTCCTGTAG TCTTAACACC AGTGGTCAAT GGAGAATCCA ATAACCTCAC GCCATCTGTT 
CCTTATCCTG CTGCTTCTCT AGTTTCTCAG AATCAGAATG AAAATGAAGG CCACCTAAAT 
CCAACTGAAA AACTCCAGAA GTTAAATGAA GTTCGAAAGA GGTTGAATGA ACTAAGAGAA 
TTAGTTCATT ATTATGAGCA AACATCAGAT ATGATGACAG ATGCTGTGAA TGAAAACACA 
AAAGAAGAAG AAACTGAAGA ATCAGAATAT GATTCGGAGC ATGAAAACTC GGAACCTGTT 
ACTAACATTC GAAATCCACA AGTGGCTGCC ACCTGGAACG AAGTAAATAG TAATTCTAAT 
GCACAGTGTG TTTCCAACAA TAGAGATGGG CGAGCAGTTA ATTCTAACTG TGAAATTAAC 
AACAGGTCTG CTGCCAACAT AAGGGCTCTA AACATGCCTC CTCTTTCAGC AGATTGTCGA 
TACAATAGAG AAGGAGAACA GGAGATGCCT GTTGCACAAG GTCATGAGGA GGAGGAGGAG 
GAGGAGGAGG AAGCAGAAGA TGAGGCCCTC AGTGGAGCTT CACTGTCTAG TCACAGCAGC 
AGCCTGATCA TTGAGACTCC TGAAGATGCT GAATTTGAAC ATAAGATCCA GAAGCTTATG 
GCTGCAAAAC TGAAACTTAG ACACTTACAG AACCTTGTAG CCATGGTGCA GGACGATGAT 
GCAGCCAATC AAGGAGTTAC TTCTGTGGAG GATTTCTTCC CAGCAGAAGA CACCAAACAA 
AATGCAAATA ACACTAGAGG AAATGCCAAT AAAACACAGA AAGATGCTGG AGTAAATGAA 
AAGGCAAGGG AGAAATTTTA TGAGGCTAAA CTACAGCAGC AACAGAGAGA ACTCAAACAA 
TTGCAGGAAG AAAGAAAGAA ACTGATTGAG ATTCAAGAGA AAATTCAAGA ATTGCAAAAG 
GCATGTCCTA ACCTACAGCT GTCAGCTGCT AGTGTGGGTA ATTGTCCCAC AAAAACACAT 
ATGCCAGCTG TTACTTCATC CCCAACCGTT AATGAAAATG AAGCCAGTAC AAACAAATCT 
GGTTTTGAGC CTCATGATTC TTCAGTGGTA GATAATGAGG TATGGTCAGA TATGCGAAGA 
CATGAAATGT TAAGGGAGGA GCTACGACAG AGAAGAAAGC AGCTGGAAGC TCTGATGGCT 
GAACATCAGA GGAGGCAAGG TCTGGCTGAA ACTGCATCTC CAGTGGCTGT TTCACTGAGA 
AGTGATGGAT CTGAGAACCT GTGTACACCT CAGCAAAGCA GAACAGAAAA AACAATGGCA 
ACTTGGGGAG GTTCTACCCA GTGTGCCCTA GATGAAGAAG GAGATGAAGA TGGTTACCTT 
TCTGAAGGAA TTATTCGGAC AGACGAAGAG GAGGAAGAGG AGGAGCAAGA TGCCAGTTCC 
AATGATAACT TTCATATGTA TCCTCCAAAC AGTGCAAATC ATAATTCTTA CAATGTAAAG 
GAAACTAAAA ATAGGTGGAA GAACAATCGC CCTTTTTCAG CAGATGGAAA TTATCGTCCT 
TTAGCCAAGA CAAGACAGCA GAATATCAGC ATGCAGCGTC AGGAAAACCT TCGCTGGGTG 
TCCGAACTCT CTTATGTAGA AGAGAAAGAA CAATGGCAAG AACAAATTAA TCAGCTAAAG 
AAACAACTTG ATTTCAGTGT CAATATTTGT CAGACTTTAA TGCAAGACCA GCAGACTCTG 
TCTTGTCTGC TACAAACTCT TCTCACGAGT CCTTATAGTG TTATGCCCAG CAGTGTTGCA 
TCCCCGCAAG TGCACCTTAT AATGCACCAG TTGAATCAGT GCTATACCCA GCTAACGTGG 
CAGCAGAACA ATGTCCAGAG GTTGAAACAA ATGCTCAGTG AACTCATGCG CCAACAAAAT 
CAGCATCCAG AAAAACCTGC AAGCAAGGAA GGAGGCAGTG GTACATCACA CCCTCCTTCT 
CCCAGTTTAT TTTGTCCTTT CAACTTTCCA ACTCAGCCTG TAAATCTGTT TAACCTACCG 
GGGTTTACTA ATTTTTCGTC ATTTGCACCA AGTATGAATT TCAGCCCTTT ATTTCCTTCT 
AATTTTGGAG ATTTTTCTCA AAATATTTCT ACACCCACTG AACAGCAGCA ACCGTTAGCC 
CAGAATCCTT CAGGGAAAAC GGAATACATG GCTTTTCCAA AACCTTTTGA AAGTAGTTCT 
TCTATTGGAG CGGAAAAACA AAGGAATCAA AAACAGCCTG AAGAGGAGGT GGAGAACAGT 
AGGACACCAT GGTTTTATGA CCAAGAAGGT GAAGTAGAAA AGCCATTTCT CAAGACTGGA 
TTTGCAGTGT CTGTAGAGAA AACAACAAAT AGTAATCGCA AAAATCAACT AGATACCAGT 
AGGAGAAGAC GCCAGTTTGA TGAAGTGTCC TTAGAAAGCT TTAACAGTAT GCTTGACCCA 
GTAGATCCAA CAACAGTAAC TAAAACATTC AAGTCAAGAA AAGCATCTGC ACAGGCCAGC 
CTGGCATCTA AAGATAAAAC TCCCAAATCA AAGAGTAAGA AGAGGCATTC TACTCAGCTG 
AAAAGCAGAG TGAAAAATAT CGAAACCGGG AGTGATTTTT CCATGTTCGA AGCTTTGCGG 
GATACCATTT ATTCTGAAGT AGCTACGTTG ATTTCTCAAA ATGAATCTCG TCCACATTTT 
CTTATTGAAC TCTTCCATGA GCTTCAGCTA CTTAATACAG ACTATTTGAG ACAGAGGGCC 
TTATATGCAT TGCAGGACAT AGTATCCAGG CATATTTCTG AGAACCATGA AAAAGAAGGA 
GAAAATATAA AGTCCGTGAA TTCTGGTACT TGGATAGCAT CCAACTCAGA ACTTACTCCC 
AGTGAAAGCC TTGCTACTAC TGATGATGAA ACTTTTGAGA AGAACTTTGA GAGAGAAACA 
CATAAAATAA GTGAGCAAAA TGATGCTGAT AATGCTAGTG TATCTTCAAC TTGTGAGCCT 
TTTGCAACAG ATGTTCTGGG CGACACTGTG ATTCACTTAG ATCAGGCATT AGCCAGAATG 
AGAGACTATA CGCATATGAA GATGGAGGCT GAAAGTAGTC CAGACGTCAG GTGCACCTGC 
AGGATCATTG AGGATGACGA TGGTGCTGCT GCCACAACCA CGATTAGTAA TAATTTAGAA 
GAAACTCCTG TTATTGAAAA TCATAGTTCA CAGCAACCCG TGAGTGAAGT TGCTACCGTC 
CCGTGTCCTA GAATTGATAC TCAGCAACTG GACCGGCAAA TTAAAGCAAT TATGAAAGAA 
GTCATTCCTT TTTTGAAGGA GCACATGGAT GAAGTATGTT CTTCTCAACT TCTAACCTCA 
GTAAGACGCA TGGTTTTGGT CCTTACTCAG CAAAACAATG AGAGCAAAGA GTTTGTAAAG 
TTCTTTCATA AGCAGCTTGG AAGTATATTA CAGGATTCAC TCGCAAAATT TGCTGGCAGA 
AAACTGAAGG ATTGTGGAGA AGATCTTCTT GTAGAGATAT CTGAAGTGTT ATTTAATGAA 
TTGGCTTTCT TTAAGCTCAT GCAAGATTTG GATAATAATA GTGTAACTGT TAAACAGAGA 
TGCAAAAGGA AAATAGAAGC TGCTGGAGTG ATACAGTCTT ATGCAAAAGA GGCCAAAAGG 
ATTCTTGAAG GAGATCACAG TTCACCTGCT GGAGAAATTG ATGATGAAGA CAAAGACAAG 
GATGAGACGG AAACAGTTAA GCAGACTCAA ACGTCTGAGG TTTATGATGG TGACGGCCCC 
AAAAATGTGA GATCTGATGT TTCTGATCAA GAGGATGATG AAGAAAGTGA AGAATGTCCA 
GTGTCTATTA ATTTGTCCAA AGCTGAAACT CAGGCTTTGA CTAATTACGG AAGTGGAGAA 
GATGAGAATG AAGATGAAGA AATAGAAGAA TTTGAAGAGG GACCTGTAGA TGTCCAGACG 
TCACTTCAGG CTAACACTGA AACTACAGAA GAAAATGAAC ACGATGATCA GGTTCCACAG 
CATGATTTTG AAAAATCATC AGAAAGCAAA AATGTCCCAT CAGAACAAGA ACCCACTACT 
AGTAAAGACT TTCTTTTGAT GACAGGTGAC CAGAATAGCA CTCCTGTGAA GCCCTGTTAC 
CTCAGTATCG TGGAAAATGA GCAACCTTTA AATAGCCCTG TCCAGAAGGA CACTCTCACT 
ACCATCGACT CATCCAGTCA GCCTAACCCT TTGCCTTTAC CTTTAACTGA AATTGAAACC 
TTGGTGCCTA GAGTCAAAGA AGTGAAATCT GCTCAGGAGA CTCCTGAAAG TTCTTTGGCT 
GGAAGTCCTG ATACTGAATC TCCAGTGTTA GTGAATGACT ATGAAGCAGA ATCTGGTAAC 
ATAAGTCAAA AATCTGATGA AGAAGACTTT GTGAAAGTTG AAGATTTACC ACTTAAACTG 
ACAATATATT CAGAGGCAGA TCTAAGGAAG AAAATGGCCG AAGAAGAACA GAAAAACCAC 
TTATCTGGTG AAATATGTGC AATGCAGACT GAAGAATTAG CTGGGAATTC TCAGACACTA 
AAAGAACCTG AGCAAGTATG TGCTCAGTCT GCCATCTTAA GATGGAAACC CTTACCTGTG 
CCTTTTAAAC TGTTGGTGGT GTCATCAGGG AATTATTGTA GCAAAAAACA GTGGGAGCCC 
AAAGTACATG AGATGTCTCC AGAGGCTCAG CTAACTCTTT CTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( XM_025279487.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025135272.1 Bubalus bubalis pericentriolar material 1 (PCM1), transcript variant X15, mRNA.

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CloneID	OBu42303
Clone ID Related Accession (Same CDS sequence)	XM_025279487.1
Accession Version	XM_025279487.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6462bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	pericentriolar material 1 protein isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037545.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG GAGGAGGTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
AGCAATGAGA GTGTTGATGA CCGACTCAAC AATATGGATT GGGGCAGCCA GCAGAAGAAG 
GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA 
ACTAACGATA TTTCTCCGGA GTCATCACCG GGAGTTGGAA GACGAAGAAC AAAGACTTCA 
CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TCCCCGAGCA GGCAGAACTA 
GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCTCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCCAC TGTAAGTCCT 
CAAAAGAGAG AAATGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAGATTCGTG AGTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGACCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTCACT 
CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT CCAGAAGATT 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCGGT CTGAGGCTTC AGATGCCACG 
GAGGCATCTT TTAGTCTGAG AATTCGGCCG TGCGTTGAGG ACAAACTAGG GAATTCAGCT 
TCACAAGCAC AGGTTTCAGA CACTGACGTT ACTACAAGTC CAAAAGGGAA AGGTGACAGA 
CCTCCTCAGC ATGACAGGGA GCTGAGGCCT AATAGGAAAT GTAGCCGAAA ACGTGGATTT 
CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATGGAAG AGATAGAAAA TTTGAAGAAA 
CAACATGATT TATTAAAAAG GATGTTACAG CAGCAGGAGC AGCTGAGAGC TCTACAAGGA 
CGACAAGCTG CTCTGCTTGC CCTGCAGCAT AAAGCTGAGC AGGCTATAGC TGTGATGGAT 
GATTCTGAAA AGGTTGCGGA GACAACTCCT GGCCATCATA CTGTACCAGG CAGACAGCCG 
GCTCATTCAC CTTTGCATCA GAGAGTACCC ATAAGAGAAA CTACAGGTAG CCTATCTGGA 
GTCAGTATCA CCTCTGAACT AAACGAAGAA TTGAATGATT TAATTCAGCA TTTTCATAAT 
CAGCTTCGTG ATTCTCAGCC TCCAGCTGTT CCAGACAATA GAAGACAAGC AGAAAGTCTT 
TCATTAACTA GGGAGATTTC TCAGAGCAGA AATCCTTCAG TTTCAGAGCA TTTACCTGAT 
GAGAAAGTCC AACTTTTTAG CAAGATGAGA GTGTTACAAG AAAAGAAACA AAAAATGGAC 
AAACTTCTTG GAGAACTTCA TACACTTCGA GATCAGCATT TGAACAATTC ATCATTTGTG 
CCTTCTTCAG CCTCTCCACA AAGGAGTGTG GATCAGAGAA GTACAACCAC AGCTCCTTCT 
GCTCCTGTAG TCTTAACACC AGTGGTCAAT GGAGAATCCA ATAACCTCAC GCCATCTGTT 
CCTTATCCTG CTGCTTCTCT AGTTTCTCAG AATCAGAATG AAAATGAAGG CCACCTAAAT 
CCAACTGAAA AACTCCAGAA GTTAAATGAA GTTCGAAAGA GGTTGAATGA ACTAAGAGAA 
TTAGTTCATT ATTATGAGCA AACATCAGAT ATGATGACAG ATGCTGTGAA TGAAAACACA 
AAAGAAGAAG AAACTGAAGA ATCAGAATAT GATTCGGAGC ATGAAAACTC GGAACCTGTT 
ACTAACATTC GAAATCCACA AGTGGCTGCC ACCTGGAACG AAGTAAATAG TAATTCTAAT 
GCACAGTGTG TTTCCAACAA TAGAGATGGG CGAGCAGTTA ATTCTAACTG TGAAATTAAC 
AACAGGTCTG CTGCCAACAT AAGGGCTCTA AACATGCCTC CTCTTTCAGA TTGTCGATAC 
AATAGAGAAG GAGAACAGGA GATGCCTGTT GCACAAGGTC ATGAGGAGGA GGAGGAGGAG 
GAGGAGGAAG CAGAAGATGA GGCCCTCAGT GGAGCTTCAC TGTCTAGTCA CAGCAGCAGC 
CTGATCATTG AGACTCCTGA AGATGCTGAA TTTGAACATA AGATCCAGAA GCTTATGGCT 
GCAAAACTGA AACTTAGACA CTTACAGAAC CTTGTAGCCA TGGTGCAGGA CGATGATGCA 
GCCAATCAAG GAGTTACTTC TGTGGAGGAT TTCTTCCCAG CAGAAGACAC CAAACAAAAT 
GCAAATAACA CTAGAGGAAA TGCCAATAAA ACACAGAAAG ATGCTGGAGT AAATGAAAAG 
GCAAGGGAGA AATTTTATGA GGCTAAACTA CAGCAGCAAC AGAGAGAACT CAAACAATTG 
CAGGAAGAAA GAAAGAAACT GATTGAGATT CAAGAGAAAA TTCAAGAATT GCAAAAGGCA 
TGTCCTAACC TACAGCTGTC AGCTGCTAGT GTGGGTAATT GTCCCACAAA AACACATATG 
CCAGCTGTTA CTTCATCCCC AACCGTTAAT GAAAATGAAG CCAGTACAAA CAAATCTGGT 
TTTGAGCCTC ATGATTCTTC AGTGGTAGAT AATGAGGTAT GGTCAGATAT GCGAAGACAT 
GAAATGTTAA GGGAGGAGCT ACGACAGAGA AGAAAGCAGC TGGAAGCTCT GATGGCTGAA 
CATCAGAGGA GGCAAGGTCT GGCTGAAACT GCATCTCCAG TGGCTGTTTC ACTGAGAAGT 
GATGGATCTG AGAACCTGTG TACACCTCAG CAAAGCAGAA CAGAAAAAAC AATGGCAACT 
TGGGGAGGTT CTACCCAGTG TGCCCTAGAT GAAGAAGGAG ATGAAGATGG TTACCTTTCT 
GAAGGAATTA TTCGGACAGA CGAAGAGGAG GAAGAGGAGG AGCAAGATGC CAGTTCCAAT 
GATAACTTTC ATATGTATCC TCCAAACAGT GCAAATCATA ATTCTTACAA TGTAAAGGAA 
ACTAAAAATA GGTGGAAGAA CAATCGCCCT TTTTCAGCAG ATGGAAATTA TCGTCCTTTA 
GCCAAGACAA GACAGCAGAA TATCAGCATG CAGCGTCAGG AAAACCTTCG CTGGGTGTCC 
GAACTCTCTT ATGTAGAAGA GAAAGAACAA TGGCAAGAAC AAATTAATCA GCTAAAGAAA 
CAACTTGATT TCAGTGTCAA TATTTGTCAG ACTTTAATGC AAGACCAGCA GACTCTGTCT 
TGTCTGCTAC AAACTCTTCT CACGAGTCCT TATAGTGTTA TGCCCAGCAG TGTTGCATCC 
CCGCAAGTGC ACCTTATAAT GCACCAGTTG AATCAGTGCT ATACCCAGCT AACGTGGCAG 
CAGAACAATG TCCAGAGGTT GAAACAAATG CTCAGTGAAC TCATGCGCCA ACAAAATCAG 
CATCCAGAAA AACCTGCAAG CAAGGAAGGA GGCAGTGGTA CATCACACCC TCCTTCTCCC 
AGTTTATTTT GTCCTTTCAA CTTTCCAACT CAGCCTGTAA ATCTGTTTAA CCTACCGGGG 
TTTACTAATT TTTCGTCATT TGCACCAAGT ATGAATTTCA GCCCTTTATT TCCTTCTAAT 
TTTGGAGATT TTTCTCAAAA TATTTCTACA CCCACTGAAC AGCAGCAACC GTTAGCCCAG 
AATCCTTCAG GGAAAACGGA ATACATGGCT TTTCCAAAAC CTTTTGAAAG TAGTTCTTCT 
ATTGGAGCGG AAAAACAAAG GAATCAAAAA CAGCCTGAAG AGGAGGTGGA GAACAGTAGG 
ACACCATGGT TTTATGACCA AGAAGGTGAA GTAGAAAAGC CATTTCTCAA GACTGGATTT 
GCAGTGTCTG TAGAGAAAAC AACAAATAGT AATCGCAAAA ATCAACTAGA TACCAGTAGG 
AGAAGACGCC AGTTTGATGA AGTGTCCTTA GAAAGCTTTA ACAGTATGCT TGACCCAGTA 
GATCCAACAA CAGTAACTAA AACATTCAAG TCAAGAAAAG CATCTGCACA GGCCAGCCTG 
GCATCTAAAG ATAAAACTCC CAAATCAAAG AGTAAGAAGA GGCATTCTAC TCAGCTGAAA 
AGCAGAGTGA AAAATATCGA AACCGGGAGT GATTTTTCCA TGTTCGAAGC TTTGCGGGAT 
ACCATTTATT CTGAAGTAGC TACGTTGATT TCTCAAAATG AATCTCGTCC ACATTTTCTT 
ATTGAACTCT TCCATGAGCT TCAGCTACTT AATACAGACT ATTTGAGACA GAGGGCCTTA 
TATGCATTGC AGGACATAGT ATCCAGGCAT ATTTCTGAGA ACCATGAAAA AGAAGGAGAA 
AATATAAAGT CCGTGAATTC TGGTACTTGG ATAGCATCCA ACTCAGAACT TACTCCCAGT 
GAAAGCCTTG CTACTACTGA TGATGAAACT TTTGAGAAGA ACTTTGAGAG AGAAACACAT 
AAAATAAGTG AGCAAAATGA TGCTGATAAT GCTAGTGTAT CTTCAACTTG TGAGCCTTTT 
GCAACAGATG TTCTGGGCGA CACTGTGATT CACTTAGATC AGGCATTAGC CAGAATGAGA 
GACTATACGC ATATGAAGAT GGAGGCTGAA AGTAGTCCAG ACGTCAGGTG CACCTGCAGG 
ATCATTGAGG ATGACGATGG TGCTGCTGCC ACAACCACGA TTAGTAATAA TTTAGAAGAA 
ACTCCTGTTA TTGAAAATCA TAGTTCACAG CAACCCGTGA GTGAAGTTGC TACCGTCCCG 
TGTCCTAGAA TTGATACTCA GCAACTGGAC CGGCAAATTA AAGCAATTAT GAAAGAAGTC 
ATTCCTTTTT TGAAGGAGCA CATGGATGAA GTATGTTCTT CTCAACTTCT AACCTCAGTA 
AGACGCATGG TTTTGGTCCT TACTCAGCAA AACAATGAGA GCAAAGAGTT TGTAAAGTTC 
TTTCATAAGC AGCTTGGAAG TATATTACAG GATTCACTCG CAAAATTTGC TGGCAGAAAA 
CTGAAGGATT GTGGAGAAGA TCTTCTTGTA GAGATATCTG AAGTGTTATT TAATGAATTG 
GCTTTCTTTA AGCTCATGCA AGATTTGGAT AATAATAGTG TAACTGTTAA ACAGAGATGC 
AAAAGGAAAA TAGAAGCTGC TGGAGTGATA CAGTCTTATG CAAAAGAGGC CAAAAGGATT 
CTTGAAGGAG ATCACAGTTC ACCTGCTGGA GAAATTGATG ATGAAGACAA AGACAAGGAT 
GAGACGGAAA CAGTTAAGCA GACTCAAACG TCTGAGGTTT ATGATGGTGA CGGCCCCAAA 
AATGTGAGAT CTGATGTTTC TGATCAAGAG GATGATGAAG AAAGTGAAGA ATGTCCAGTG 
TCTATTAATT TGTCCAAAGC TGAAACTCAG GCTTTGACTA ATTACGGAAG TGGAGAAGAT 
GAGAATGAAG ATGAAGAAAT AGAAGAATTT GAAGAGGGAC CTGTAGATGT CCAGACGTCA 
CTTCAGGCTA ACACTGAAAC TACAGAAGAA AATGAACACG ATGATCAGGT TCCACAGCAT 
GATTTTGAAA AATCATCAGA AAGCAAAAAT GTCCCATCAG AACAAGAACC CACTACTAGT 
AAAGACTTTC TTTTGATGAC AGGTGACCAG AATAGCACTC CTGTGAAGCC CTGTTACCTC 
AGTATCGTGG AAAATGAGCA ACCTTTAAAT AGCCCTGTCC AGAAGGACAC TCTCACTACC 
ATCGACTCAT CCAGTCAGCC TAACCCTTTG CCTTTACCTT TAACTGAAAT TGAAACCTTG 
GTGCCTAGAG TCAAAGAAGT GAAATCTGCT CAGGAGACTC CTGAAAGTTC TTTGGCTGGA 
AGTCCTGATA CTGAATCTCC AGTGTTAGTG AATGACTATG AAGCAGAATC TGGTAACATA 
AGTCAAAAAT CTGATGAAGA AGACTTTGTG AAAGTTGAAG ATTTACCACT TAAACTGACA 
ATATATTCAG AGGCAGATCT AAGGAAGAAA ATGGCCGAAG AAGAACAGAA AAACCACTTA 
TCTGGTGAAA TATGTGCAAT GCAGACTGAA GAATTAGCTG GGAATTCTCA GACACTAAAA 
GAACCTGAGC AAGTATGTGC TCAGTCTGCC ATCTTAAGAT GGAAACCCTT ACCTGTGCCT 
TTTAAACTGT TGGTGGTGTC ATCAGGGAAT TATTGTAGCA AAAAACAGTG GGAGCCCAAA 
GTACATGAGA TGTCTCCAGA GGCTCAGCTA ACTCTTTCTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025135272.1
CDS	392..6853
Translation	MATGGGPFEEGVNDQDLPNWSNESVDDRLNNMDWGSQQKKANRSSEKNKRKFGVESDKRVTNDISPESSPGVGRRRTKTSLTFPHSRYMTQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDATVSPQKREMIGSAQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIREYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLQKILARENEEEDVRTIDSAVGSGSVAESTSLNIDVRSEASDATEASFSLRIRPCVEDKLGNSASQAQVSDTDVTTSPKGKGDRPPQHDRELRPNRKCSRKRGFPSKARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSEKVAETTPGHHTVPGRQPAHSPLHQRVPIRETTGSLSGVSITSELNEELNDLIQHFHNQLRDSQPPAVPDNRRQAESLSLTREISQSRNPSVSEHLPDEKVQLFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSFVPSSASPQRSVDQRSTTTAPSAPVVLTPVVNGESNNLTPSVPYPAASLVSQNQNENEGHLNPTEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENTKEEETEESEYDSEHENSEPVTNIRNPQVAATWNEVNSNSNAQCVSNNRDGRAVNSNCEINNRSAANIRALNMPPLSDCRYNREGEQEMPVAQGHEEEEEEEEEAEDEALSGASLSSHSSSLIIETPEDAEFEHKIQKLMAAKLKLRHLQNLVAMVQDDDAANQGVTSVEDFFPAEDTKQNANNTRGNANKTQKDAGVNEKAREKFYEAKLQQQQRELKQLQEERKKLIEIQEKIQELQKACPNLQLSAASVGNCPTKTHMPAVTSSPTVNENEASTNKSGFEPHDSSVVDNEVWSDMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETASPVAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGIIRTDEEEEEEEQDASSNDNFHMYPPNSANHNSYNVKETKNRWKNNRPFSADGNYRPLAKTRQQNISMQRQENLRWVSELSYVEEKEQWQEQINQLKKQLDFSVNICQTLMQDQQTLSCLLQTLLTSPYSVMPSSVASPQVHLIMHQLNQCYTQLTWQQNNVQRLKQMLSELMRQQNQHPEKPASKEGGSGTSHPPSPSLFCPFNFPTQPVNLFNLPGFTNFSSFAPSMNFSPLFPSNFGDFSQNISTPTEQQQPLAQNPSGKTEYMAFPKPFESSSSIGAEKQRNQKQPEEEVENSRTPWFYDQEGEVEKPFLKTGFAVSVEKTTNSNRKNQLDTSRRRRQFDEVSLESFNSMLDPVDPTTVTKTFKSRKASAQASLASKDKTPKSKSKKRHSTQLKSRVKNIETGSDFSMFEALRDTIYSEVATLISQNESRPHFLIELFHELQLLNTDYLRQRALYALQDIVSRHISENHEKEGENIKSVNSGTWIASNSELTPSESLATTDDETFEKNFERETHKISEQNDADNASVSSTCEPFATDVLGDTVIHLDQALARMRDYTHMKMEAESSPDVRCTCRIIEDDDGAAATTTISNNLEETPVIENHSSQQPVSEVATVPCPRIDTQQLDRQIKAIMKEVIPFLKEHMDEVCSSQLLTSVRRMVLVLTQQNNESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSVTVKQRCKRKIEAAGVIQSYAKEAKRILEGDHSSPAGEIDDEDKDKDETETVKQTQTSEVYDGDGPKNVRSDVSDQEDDEESEECPVSINLSKAETQALTNYGSGEDENEDEEIEEFEEGPVDVQTSLQANTETTEENEHDDQVPQHDFEKSSESKNVPSEQEPTTSKDFLLMTGDQNSTPVKPCYLSIVENEQPLNSPVQKDTLTTIDSSSQPNPLPLPLTEIETLVPRVKEVKSAQETPESSLAGSPDTESPVLVNDYEAESGNISQKSDEEDFVKVEDLPLKLTIYSEADLRKKMAEEEQKNHLSGEICAMQTEELAGNSQTLKEPEQVCAQSAILRWKPLPVPFKLLVVSSGNYCSKKQWEPKVHEMSPEAQLTLS

Target ORF information:

RefSeq Version	XM_025279487.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis pericentriolar material 1 (PCM1), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025279487.1

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
AGCAATGAGA GTGTTGATGA CCGACTCAAC AATATGGATT GGGGCAGCCA GCAGAAGAAG 
GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA 
ACTAACGATA TTTCTCCGGA GTCATCACCG GGAGTTGGAA GACGAAGAAC AAAGACTTCA 
CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TCCCCGAGCA GGCAGAACTA 
GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCTCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCCAC TGTAAGTCCT 
CAAAAGAGAG AAATGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAGATTCGTG AGTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGACCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTCACT 
CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT CCAGAAGATT 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCGGT CTGAGGCTTC AGATGCCACG 
GAGGCATCTT TTAGTCTGAG AATTCGGCCG TGCGTTGAGG ACAAACTAGG GAATTCAGCT 
TCACAAGCAC AGGTTTCAGA CACTGACGTT ACTACAAGTC CAAAAGGGAA AGGTGACAGA 
CCTCCTCAGC ATGACAGGGA GCTGAGGCCT AATAGGAAAT GTAGCCGAAA ACGTGGATTT 
CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATGGAAG AGATAGAAAA TTTGAAGAAA 
CAACATGATT TATTAAAAAG GATGTTACAG CAGCAGGAGC AGCTGAGAGC TCTACAAGGA 
CGACAAGCTG CTCTGCTTGC CCTGCAGCAT AAAGCTGAGC AGGCTATAGC TGTGATGGAT 
GATTCTGAAA AGGTTGCGGA GACAACTCCT GGCCATCATA CTGTACCAGG CAGACAGCCG 
GCTCATTCAC CTTTGCATCA GAGAGTACCC ATAAGAGAAA CTACAGGTAG CCTATCTGGA 
GTCAGTATCA CCTCTGAACT AAACGAAGAA TTGAATGATT TAATTCAGCA TTTTCATAAT 
CAGCTTCGTG ATTCTCAGCC TCCAGCTGTT CCAGACAATA GAAGACAAGC AGAAAGTCTT 
TCATTAACTA GGGAGATTTC TCAGAGCAGA AATCCTTCAG TTTCAGAGCA TTTACCTGAT 
GAGAAAGTCC AACTTTTTAG CAAGATGAGA GTGTTACAAG AAAAGAAACA AAAAATGGAC 
AAACTTCTTG GAGAACTTCA TACACTTCGA GATCAGCATT TGAACAATTC ATCATTTGTG 
CCTTCTTCAG CCTCTCCACA AAGGAGTGTG GATCAGAGAA GTACAACCAC AGCTCCTTCT 
GCTCCTGTAG TCTTAACACC AGTGGTCAAT GGAGAATCCA ATAACCTCAC GCCATCTGTT 
CCTTATCCTG CTGCTTCTCT AGTTTCTCAG AATCAGAATG AAAATGAAGG CCACCTAAAT 
CCAACTGAAA AACTCCAGAA GTTAAATGAA GTTCGAAAGA GGTTGAATGA ACTAAGAGAA 
TTAGTTCATT ATTATGAGCA AACATCAGAT ATGATGACAG ATGCTGTGAA TGAAAACACA 
AAAGAAGAAG AAACTGAAGA ATCAGAATAT GATTCGGAGC ATGAAAACTC GGAACCTGTT 
ACTAACATTC GAAATCCACA AGTGGCTGCC ACCTGGAACG AAGTAAATAG TAATTCTAAT 
GCACAGTGTG TTTCCAACAA TAGAGATGGG CGAGCAGTTA ATTCTAACTG TGAAATTAAC 
AACAGGTCTG CTGCCAACAT AAGGGCTCTA AACATGCCTC CTCTTTCAGA TTGTCGATAC 
AATAGAGAAG GAGAACAGGA GATGCCTGTT GCACAAGGTC ATGAGGAGGA GGAGGAGGAG 
GAGGAGGAAG CAGAAGATGA GGCCCTCAGT GGAGCTTCAC TGTCTAGTCA CAGCAGCAGC 
CTGATCATTG AGACTCCTGA AGATGCTGAA TTTGAACATA AGATCCAGAA GCTTATGGCT 
GCAAAACTGA AACTTAGACA CTTACAGAAC CTTGTAGCCA TGGTGCAGGA CGATGATGCA 
GCCAATCAAG GAGTTACTTC TGTGGAGGAT TTCTTCCCAG CAGAAGACAC CAAACAAAAT 
GCAAATAACA CTAGAGGAAA TGCCAATAAA ACACAGAAAG ATGCTGGAGT AAATGAAAAG 
GCAAGGGAGA AATTTTATGA GGCTAAACTA CAGCAGCAAC AGAGAGAACT CAAACAATTG 
CAGGAAGAAA GAAAGAAACT GATTGAGATT CAAGAGAAAA TTCAAGAATT GCAAAAGGCA 
TGTCCTAACC TACAGCTGTC AGCTGCTAGT GTGGGTAATT GTCCCACAAA AACACATATG 
CCAGCTGTTA CTTCATCCCC AACCGTTAAT GAAAATGAAG CCAGTACAAA CAAATCTGGT 
TTTGAGCCTC ATGATTCTTC AGTGGTAGAT AATGAGGTAT GGTCAGATAT GCGAAGACAT 
GAAATGTTAA GGGAGGAGCT ACGACAGAGA AGAAAGCAGC TGGAAGCTCT GATGGCTGAA 
CATCAGAGGA GGCAAGGTCT GGCTGAAACT GCATCTCCAG TGGCTGTTTC ACTGAGAAGT 
GATGGATCTG AGAACCTGTG TACACCTCAG CAAAGCAGAA CAGAAAAAAC AATGGCAACT 
TGGGGAGGTT CTACCCAGTG TGCCCTAGAT GAAGAAGGAG ATGAAGATGG TTACCTTTCT 
GAAGGAATTA TTCGGACAGA CGAAGAGGAG GAAGAGGAGG AGCAAGATGC CAGTTCCAAT 
GATAACTTTC ATATGTATCC TCCAAACAGT GCAAATCATA ATTCTTACAA TGTAAAGGAA 
ACTAAAAATA GGTGGAAGAA CAATCGCCCT TTTTCAGCAG ATGGAAATTA TCGTCCTTTA 
GCCAAGACAA GACAGCAGAA TATCAGCATG CAGCGTCAGG AAAACCTTCG CTGGGTGTCC 
GAACTCTCTT ATGTAGAAGA GAAAGAACAA TGGCAAGAAC AAATTAATCA GCTAAAGAAA 
CAACTTGATT TCAGTGTCAA TATTTGTCAG ACTTTAATGC AAGACCAGCA GACTCTGTCT 
TGTCTGCTAC AAACTCTTCT CACGAGTCCT TATAGTGTTA TGCCCAGCAG TGTTGCATCC 
CCGCAAGTGC ACCTTATAAT GCACCAGTTG AATCAGTGCT ATACCCAGCT AACGTGGCAG 
CAGAACAATG TCCAGAGGTT GAAACAAATG CTCAGTGAAC TCATGCGCCA ACAAAATCAG 
CATCCAGAAA AACCTGCAAG CAAGGAAGGA GGCAGTGGTA CATCACACCC TCCTTCTCCC 
AGTTTATTTT GTCCTTTCAA CTTTCCAACT CAGCCTGTAA ATCTGTTTAA CCTACCGGGG 
TTTACTAATT TTTCGTCATT TGCACCAAGT ATGAATTTCA GCCCTTTATT TCCTTCTAAT 
TTTGGAGATT TTTCTCAAAA TATTTCTACA CCCACTGAAC AGCAGCAACC GTTAGCCCAG 
AATCCTTCAG GGAAAACGGA ATACATGGCT TTTCCAAAAC CTTTTGAAAG TAGTTCTTCT 
ATTGGAGCGG AAAAACAAAG GAATCAAAAA CAGCCTGAAG AGGAGGTGGA GAACAGTAGG 
ACACCATGGT TTTATGACCA AGAAGGTGAA GTAGAAAAGC CATTTCTCAA GACTGGATTT 
GCAGTGTCTG TAGAGAAAAC AACAAATAGT AATCGCAAAA ATCAACTAGA TACCAGTAGG 
AGAAGACGCC AGTTTGATGA AGTGTCCTTA GAAAGCTTTA ACAGTATGCT TGACCCAGTA 
GATCCAACAA CAGTAACTAA AACATTCAAG TCAAGAAAAG CATCTGCACA GGCCAGCCTG 
GCATCTAAAG ATAAAACTCC CAAATCAAAG AGTAAGAAGA GGCATTCTAC TCAGCTGAAA 
AGCAGAGTGA AAAATATCGA AACCGGGAGT GATTTTTCCA TGTTCGAAGC TTTGCGGGAT 
ACCATTTATT CTGAAGTAGC TACGTTGATT TCTCAAAATG AATCTCGTCC ACATTTTCTT 
ATTGAACTCT TCCATGAGCT TCAGCTACTT AATACAGACT ATTTGAGACA GAGGGCCTTA 
TATGCATTGC AGGACATAGT ATCCAGGCAT ATTTCTGAGA ACCATGAAAA AGAAGGAGAA 
AATATAAAGT CCGTGAATTC TGGTACTTGG ATAGCATCCA ACTCAGAACT TACTCCCAGT 
GAAAGCCTTG CTACTACTGA TGATGAAACT TTTGAGAAGA ACTTTGAGAG AGAAACACAT 
AAAATAAGTG AGCAAAATGA TGCTGATAAT GCTAGTGTAT CTTCAACTTG TGAGCCTTTT 
GCAACAGATG TTCTGGGCGA CACTGTGATT CACTTAGATC AGGCATTAGC CAGAATGAGA 
GACTATACGC ATATGAAGAT GGAGGCTGAA AGTAGTCCAG ACGTCAGGTG CACCTGCAGG 
ATCATTGAGG ATGACGATGG TGCTGCTGCC ACAACCACGA TTAGTAATAA TTTAGAAGAA 
ACTCCTGTTA TTGAAAATCA TAGTTCACAG CAACCCGTGA GTGAAGTTGC TACCGTCCCG 
TGTCCTAGAA TTGATACTCA GCAACTGGAC CGGCAAATTA AAGCAATTAT GAAAGAAGTC 
ATTCCTTTTT TGAAGGAGCA CATGGATGAA GTATGTTCTT CTCAACTTCT AACCTCAGTA 
AGACGCATGG TTTTGGTCCT TACTCAGCAA AACAATGAGA GCAAAGAGTT TGTAAAGTTC 
TTTCATAAGC AGCTTGGAAG TATATTACAG GATTCACTCG CAAAATTTGC TGGCAGAAAA 
CTGAAGGATT GTGGAGAAGA TCTTCTTGTA GAGATATCTG AAGTGTTATT TAATGAATTG 
GCTTTCTTTA AGCTCATGCA AGATTTGGAT AATAATAGTG TAACTGTTAA ACAGAGATGC 
AAAAGGAAAA TAGAAGCTGC TGGAGTGATA CAGTCTTATG CAAAAGAGGC CAAAAGGATT 
CTTGAAGGAG ATCACAGTTC ACCTGCTGGA GAAATTGATG ATGAAGACAA AGACAAGGAT 
GAGACGGAAA CAGTTAAGCA GACTCAAACG TCTGAGGTTT ATGATGGTGA CGGCCCCAAA 
AATGTGAGAT CTGATGTTTC TGATCAAGAG GATGATGAAG AAAGTGAAGA ATGTCCAGTG 
TCTATTAATT TGTCCAAAGC TGAAACTCAG GCTTTGACTA ATTACGGAAG TGGAGAAGAT 
GAGAATGAAG ATGAAGAAAT AGAAGAATTT GAAGAGGGAC CTGTAGATGT CCAGACGTCA 
CTTCAGGCTA ACACTGAAAC TACAGAAGAA AATGAACACG ATGATCAGGT TCCACAGCAT 
GATTTTGAAA AATCATCAGA AAGCAAAAAT GTCCCATCAG AACAAGAACC CACTACTAGT 
AAAGACTTTC TTTTGATGAC AGGTGACCAG AATAGCACTC CTGTGAAGCC CTGTTACCTC 
AGTATCGTGG AAAATGAGCA ACCTTTAAAT AGCCCTGTCC AGAAGGACAC TCTCACTACC 
ATCGACTCAT CCAGTCAGCC TAACCCTTTG CCTTTACCTT TAACTGAAAT TGAAACCTTG 
GTGCCTAGAG TCAAAGAAGT GAAATCTGCT CAGGAGACTC CTGAAAGTTC TTTGGCTGGA 
AGTCCTGATA CTGAATCTCC AGTGTTAGTG AATGACTATG AAGCAGAATC TGGTAACATA 
AGTCAAAAAT CTGATGAAGA AGACTTTGTG AAAGTTGAAG ATTTACCACT TAAACTGACA 
ATATATTCAG AGGCAGATCT AAGGAAGAAA ATGGCCGAAG AAGAACAGAA AAACCACTTA 
TCTGGTGAAA TATGTGCAAT GCAGACTGAA GAATTAGCTG GGAATTCTCA GACACTAAAA 
GAACCTGAGC AAGTATGTGC TCAGTCTGCC ATCTTAAGAT GGAAACCCTT ACCTGTGCCT 
TTTAAACTGT TGGTGGTGTC ATCAGGGAAT TATTGTAGCA AAAAACAGTG GGAGCCCAAA 
GTACATGAGA TGTCTCCAGA GGCTCAGCTA ACTCTTTCTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( XM_025279491.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025135276.1 Bubalus bubalis pericentriolar material 1 (PCM1), transcript variant X16, mRNA.

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CloneID	OBu42304
Clone ID Related Accession (Same CDS sequence)	XM_025279491.1
Accession Version	XM_025279491.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6381bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	pericentriolar material 1 protein isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037545.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG GAGGAGGTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
AGCAATGAGA GTGTTGATGA CCGACTCAAC AATATGGATT GGGGCAGCCA GCAGAAGAAG 
GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA 
ACTAACGATA TTTCTCCGGA GTCATCACCG GGAGTTGGAA GACGAAGAAC AAAGACTTCA 
CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TCCCCGAGCA GGCAGAACTA 
GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCTCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCCAC TGTAAGTCCT 
CAAAAGAGAG AAATGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAGATTCGTG AGTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGACCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTCACT 
CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT CCAGAAGATT 
CTTGCCAGAG ATCCTCAGCA GGAGCCTATG GAAGAGATAG AAAATTTGAA GAAACAACAT 
GATTTATTAA AAAGGATGTT ACAGCAGCAG GAGCAGCTGA GAGCTCTACA AGGACGACAA 
GCTGCTCTGC TTGCCCTGCA GCATAAAGCT GAGCAGGCTA TAGCTGTGAT GGATGATTCT 
GAAAAGGTTG CGGAGACAAC TCCTGGCCAT CATACTGTAC CAGGCAGACA GCCGGCTCAT 
TCACCTTTGC ATCAGAGAGT ACCCATAAGA GAAACTACAG GTAGCCTATC TGGAGTCAGT 
ATCACCTCTG AACTAAACGA AGAATTGAAT GATTTAATTC AGCATTTTCA TAATCAGCTT 
CGTGATTCTC AGCCTCCAGC TGTTCCAGAC AATAGAAGAC AAGCAGAAAG TCTTTCATTA 
ACTAGGGAGA TTTCTCAGAG CAGAAATCCT TCAGTTTCAG AGCATTTACC TGATGAGAAA 
GTCCAACTTT TTAGCAAGAT GAGAGTGTTA CAAGAAAAGA AACAAAAAAT GGACAAACTT 
CTTGGAGAAC TTCATACACT TCGAGATCAG CATTTGAACA ATTCATCATT TGTGCCTTCT 
TCAGCCTCTC CACAAAGGAG TGTGGATCAG AGAAGTACAA CCACAGCTCC TTCTGCTCCT 
GTAGTCTTAA CACCAGTGGT CAATGGAGAA TCCAATAACC TCACGCCATC TGTTCCTTAT 
CCTGCTGCTT CTCTAGTTTC TCAGAATCAG AATGAAAATG AAGGCCACCT AAATCCAACT 
GAAAAACTCC AGAAGTTAAA TGAAGTTCGA AAGAGGTTGA ATGAACTAAG AGAATTAGTT 
CATTATTATG AGCAAACATC AGATATGATG ACAGATGCTG TGAATGAAAA CACAAAAGAA 
GAAGAAACTG AAGAATCAGA ATATGATTCG GAGCATGAAA ACTCGGAACC TGTTACTAAC 
ATTCGAAATC CACAAGTGGC TGCCACCTGG AACGAAGTAA ATAGTAATTC TAATGCACAG 
TGTGTTTCCA ACAATAGAGA TGGGCGAGCA GTTAATTCTA ACTGTGAAAT TAACAACAGG 
TCTGCTGCCA ACATAAGGGC TCTAAACATG CCTCCTCTTT CAGCAGATTG TCGATACAAT 
AGAGAAGGAG AACAGGAGAT GCCTGTTGCA CAAGGTCATG AGGAGGAGGA GGAGGAGGAG 
GAGGAAGCAG AAGATGAGGC CCTCAGTGGA GCTTCACTGT CTAGTCACAG CAGCAGCCTG 
ATCATTGAGA CTCCTGAAGA TGCTGAATTT GAACATAAGA TCCAGAAGCT TATGGCTGCA 
AAACTGAAAC TTAGACACTT ACAGAACCTT GTAGCCATGG TGCAGGACGA TGATGCAGCC 
AATCAAGGAG TTACTTCTGT GGAGGATTTC TTCCCAGCAG AAGACACCAA ACAAAATGCA 
AATAACACTA GAGGAAATGC CAATAAAACA CAGAAAGATG CTGGAGTAAA TGAAAAGGCA 
AGGGAGAAAT TTTATGAGGC TAAACTACAG CAGCAACAGA GAGAACTCAA ACAATTGCAG 
GAAGAAAGAA AGAAACTGAT TGAGATTCAA GAGAAAATTC AAGAATTGCA AAAGGCATGT 
CCTAACCTAC AGCTGTCAGC TGCTAGTGTG GGTAATTGTC CCACAAAAAC ACATATGCCA 
GCTGTTACTT CATCCCCAAC CGTTAATGAA AATGAAGCCA GTACAAACAA ATCTGGTTTT 
GAGCCTCATG ATTCTTCAGT GGTAGATAAT GAGGTATGGT CAGATATGCG AAGACATGAA 
ATGTTAAGGG AGGAGCTACG ACAGAGAAGA AAGCAGCTGG AAGCTCTGAT GGCTGAACAT 
CAGAGGAGGC AAGGTCTGGC TGAAACTGCA TCTCCAGTGG CTGTTTCACT GAGAAGTGAT 
GGATCTGAGA ACCTGTGTAC ACCTCAGCAA AGCAGAACAG AAAAAACAAT GGCAACTTGG 
GGAGGTTCTA CCCAGTGTGC CCTAGATGAA GAAGGAGATG AAGATGGTTA CCTTTCTGAA 
GGAATTATTC GGACAGACGA AGAGGAGGAA GAGGAGGAGC AAGATGCCAG TTCCAATGAT 
AACTTTCATA TGTATCCTCC AAACAGTGCA AATCATAATT CTTACAATGT AAAGGAAACT 
AAAAATAGGT GGAAGAACAA TCGCCCTTTT TCAGCAGATG GAAATTATCG TCCTTTAGCC 
AAGACAAGAC AGCAGAATAT CAGCATGCAG CGTCAGGAAA ACCTTCGCTG GGTGTCCGAA 
CTCTCTTATG TAGAAGAGAA AGAACAATGG CAAGAACAAA TTAATCAGCT AAAGAAACAA 
CTTGATTTCA GTGTCAATAT TTGTCAGACT TTAATGCAAG ACCAGCAGAC TCTGTCTTGT 
CTGCTACAAA CTCTTCTCAC GAGTCCTTAT AGTGTTATGC CCAGCAGTGT TGCATCCCCG 
CAAGTGCACC TTATAATGCA CCAGTTGAAT CAGTGCTATA CCCAGCTAAC GTGGCAGCAG 
AACAATGTCC AGAGGTTGAA ACAAATGCTC AGTGAACTCA TGCGCCAACA AAATCAGCAT 
CCAGAAAAAC CTGCAAGCAA GGAAGGAGGC AGTGGTACAT CACACCCTCC TTCTCCCAGT 
TTATTTTGTC CTTTCAACTT TCCAACTCAG CCTGTAAATC TGTTTAACCT ACCGGGGTTT 
ACTAATTTTT CGTCATTTGC ACCAAGTATG AATTTCAGCC CTTTATTTCC TTCTAATTTT 
GGAGATTTTT CTCAAAATAT TTCTACACCC ACTGAACAGC AGCAACCGTT AGCCCAGAAT 
CCTTCAGGGA AAACGGAATA CATGGCTTTT CCAAAACCTT TTGAAAGTAG TTCTTCTATT 
GGAGCGGAAA AACAAAGGAA TCAAAAACAG CCTGAAGAGG AGGTGGAGAA CAGTAGGACA 
CCATGGTTTT ATGACCAAGA AGGTGAAGTA GAAAAGCCAT TTCTCAAGAC TGGATTTGCA 
GTGTCTGTAG AGAAAACAAC AAATAGTAAT CGCAAAAATC AACTAGATAC CAGTAGGAGA 
AGACGCCAGT TTGATGAAGT GTCCTTAGAA AGCTTTAACA GTATGCTTGA CCCAGTAGAT 
CCAACAACAG TAACTAAAAC ATTCAAGTCA AGAAAAGCAT CTGCACAGGC CAGCCTGGCA 
TCTAAAGATA AAACTCCCAA ATCAAAGAGT AAGAAGAGGC ATTCTACTCA GCTGAAAAGC 
AGAGTGAAAA ATATCGGGTA TGAAAGTGCC AGTGTGTCTA GCACATGTGA ACCTTGCAAA 
AGTAGAAACA GACATTCAGC CCAAACTGAA GAGCCTGTTC AAGCAAAAGT ACTCAGCAGA 
AAGAATCATG AGCAGCTGGA AAAAGTCATA AGATGTAGTA GGTCTACAGA AATATCTTCA 
GCACATGCTA GGAGAATACT ACAGCAGTCT AACAGAAATG CATGCAATGA AGCGCCAGAA 
ACCGGGAGTG ATTTTTCCAT GTTCGAAGCT TTGCGGGATA CCATTTATTC TGAAGTAGCT 
ACGTTGATTT CTCAAAATGA ATCTCGTCCA CATTTTCTTA TTGAACTCTT CCATGAGCTT 
CAGCTACTTA ATACAGACTA TTTGAGACAG AGGGCCTTAT ATGCATTGCA GGACATAGTA 
TCCAGGCATA TTTCTGAGAA CCATGAAAAA GAAGGAGAAA ATATAAAGTC CGTGAATTCT 
GGTACTTGGA TAGCATCCAA CTCAGAACTT ACTCCCAGTG AAAGCCTTGC TACTACTGAT 
GATGAAACTT TTGAGAAGAA CTTTGAGAGA GAAACACATA AAATAAGTGA GCAAAATGAT 
GCTGATAATG CTAGTGTATC TTCAACTTGT GAGCCTTTTG CAACAGATGT TCTGGGCGAC 
ACTGTGATTC ACTTAGATCA GGCATTAGCC AGAATGAGAG ACTATACGCA TATGAAGATG 
GAGGCTGAAA GTAGTCCAGA CGTCAGGTGC ACCTGCAGGA TCATTGAGGA TGACGATGGT 
GCTGCTGCCA CAACCACGAT TAGTAATAAT TTAGAAGAAA CTCCTGTTAT TGAAAATCAT 
AGTTCACAGC AACCCGTGAG TGAAGTTGCT ACCGTCCCGT GTCCTAGAAT TGATACTCAG 
CAACTGGACC GGCAAATTAA AGCAATTATG AAAGAAGTCA TTCCTTTTTT GAAGGAGCAC 
ATGGATGAAG TATGTTCTTC TCAACTTCTA ACCTCAGTAA GACGCATGGT TTTGGTCCTT 
ACTCAGCAAA ACAATGAGAG CAAAGAGTTT GTAAAGTTCT TTCATAAGCA GCTTGGAAGT 
ATATTACAGG ATTCACTCGC AAAATTTGCT GGCAGAAAAC TGAAGGATTG TGGAGAAGAT 
CTTCTTGTAG AGATATCTGA AGTGTTATTT AATGAATTGG CTTTCTTTAA GCTCATGCAA 
GATTTGGATA ATAATAGTGT AACTGTTAAA CAGAGATGCA AAAGGAAAAT AGAAGCTGCT 
GGAGTGATAC AGTCTTATGC AAAAGAGGCC AAAAGGATTC TTGAAGGAGA TCACAGTTCA 
CCTGCTGGAG AAATTGATGA TGAAGACAAA GACAAGGATG AGACGGAAAC AGTTAAGCAG 
ACTCAAACGT CTGAGGTTTA TGATGGTGAC GGCCCCAAAA ATGTGAGATC TGATGTTTCT 
GATCAAGAGG ATGATGAAGA AAGTGAAGAA TGTCCAGTGT CTATTAATTT GTCCAAAGCT 
GAAACTCAGG CTTTGACTAA TTACGGAAGT GGAGAAGATG AGAATGAAGA TGAAGAAATA 
GAAGAATTTG AAGAGGGACC TGTAGATGTC CAGACGTCAC TTCAGGCTAA CACTGAAACT 
ACAGAAGAAA ATGAACACGA TGATCAGGTT CCACAGCATG ATTTTGAAAA ATCATCAGAA 
AGCAAAAATG TCCCATCAGA ACAAGAACCC ACTACTAGTA AAGACTTTCT TTTGATGACA 
GGTGACCAGA ATAGCACTCC TGTGAAGCCC TGTTACCTCA GTATCGTGGA AAATGAGCAA 
CCTTTAAATA GCCCTGTCCA GAAGGACACT CTCACTACCA TCGACTCATC CAGTCAGCCT 
AACCCTTTGC CTTTACCTTT AACTGAAATT GAAACCTTGG TGCCTAGAGT CAAAGAAGTG 
AAATCTGCTC AGGAGACTCC TGAAAGTTCT TTGGCTGGAA GTCCTGATAC TGAATCTCCA 
GTGTTAGTGA ATGACTATGA AGCAGAATCT GGTAACATAA GTCAAAAATC TGATGAAGAA 
GACTTTGTGA AAGTTGAAGA TTTACCACTT AAACTGACAA TATATTCAGA GGCAGATCTA 
AGGAAGAAAA TGGCCGAAGA AGAACAGAAA AACCACTTAT CTGGTGAAAT ATGTGCAATG 
CAGACTGAAG AATTAGCTGG GAATTCTCAG ACACTAAAAG AACCTGAGCA AGTATGTGCT 
CAGTCTGCCA TCTTAAGATG GAAACCCTTA CCTGTGCCTT TTAAACTGTT GGTGGTGTCA 
TCAGGGAATT ATTGTAGCAA AAAACAGTGG GAGCCCAAAG TACATGAGAT GTCTCCAGAG 
GCTCAGCTAA CTCTTTCTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025135276.1
CDS	392..6772
Translation	MATGGGPFEEGVNDQDLPNWSNESVDDRLNNMDWGSQQKKANRSSEKNKRKFGVESDKRVTNDISPESSPGVGRRRTKTSLTFPHSRYMTQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDATVSPQKREMIGSAQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIREYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLQKILARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSEKVAETTPGHHTVPGRQPAHSPLHQRVPIRETTGSLSGVSITSELNEELNDLIQHFHNQLRDSQPPAVPDNRRQAESLSLTREISQSRNPSVSEHLPDEKVQLFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSFVPSSASPQRSVDQRSTTTAPSAPVVLTPVVNGESNNLTPSVPYPAASLVSQNQNENEGHLNPTEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENTKEEETEESEYDSEHENSEPVTNIRNPQVAATWNEVNSNSNAQCVSNNRDGRAVNSNCEINNRSAANIRALNMPPLSADCRYNREGEQEMPVAQGHEEEEEEEEEAEDEALSGASLSSHSSSLIIETPEDAEFEHKIQKLMAAKLKLRHLQNLVAMVQDDDAANQGVTSVEDFFPAEDTKQNANNTRGNANKTQKDAGVNEKAREKFYEAKLQQQQRELKQLQEERKKLIEIQEKIQELQKACPNLQLSAASVGNCPTKTHMPAVTSSPTVNENEASTNKSGFEPHDSSVVDNEVWSDMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETASPVAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGIIRTDEEEEEEEQDASSNDNFHMYPPNSANHNSYNVKETKNRWKNNRPFSADGNYRPLAKTRQQNISMQRQENLRWVSELSYVEEKEQWQEQINQLKKQLDFSVNICQTLMQDQQTLSCLLQTLLTSPYSVMPSSVASPQVHLIMHQLNQCYTQLTWQQNNVQRLKQMLSELMRQQNQHPEKPASKEGGSGTSHPPSPSLFCPFNFPTQPVNLFNLPGFTNFSSFAPSMNFSPLFPSNFGDFSQNISTPTEQQQPLAQNPSGKTEYMAFPKPFESSSSIGAEKQRNQKQPEEEVENSRTPWFYDQEGEVEKPFLKTGFAVSVEKTTNSNRKNQLDTSRRRRQFDEVSLESFNSMLDPVDPTTVTKTFKSRKASAQASLASKDKTPKSKSKKRHSTQLKSRVKNIGYESASVSSTCEPCKSRNRHSAQTEEPVQAKVLSRKNHEQLEKVIRCSRSTEISSAHARRILQQSNRNACNEAPETGSDFSMFEALRDTIYSEVATLISQNESRPHFLIELFHELQLLNTDYLRQRALYALQDIVSRHISENHEKEGENIKSVNSGTWIASNSELTPSESLATTDDETFEKNFERETHKISEQNDADNASVSSTCEPFATDVLGDTVIHLDQALARMRDYTHMKMEAESSPDVRCTCRIIEDDDGAAATTTISNNLEETPVIENHSSQQPVSEVATVPCPRIDTQQLDRQIKAIMKEVIPFLKEHMDEVCSSQLLTSVRRMVLVLTQQNNESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSVTVKQRCKRKIEAAGVIQSYAKEAKRILEGDHSSPAGEIDDEDKDKDETETVKQTQTSEVYDGDGPKNVRSDVSDQEDDEESEECPVSINLSKAETQALTNYGSGEDENEDEEIEEFEEGPVDVQTSLQANTETTEENEHDDQVPQHDFEKSSESKNVPSEQEPTTSKDFLLMTGDQNSTPVKPCYLSIVENEQPLNSPVQKDTLTTIDSSSQPNPLPLPLTEIETLVPRVKEVKSAQETPESSLAGSPDTESPVLVNDYEAESGNISQKSDEEDFVKVEDLPLKLTIYSEADLRKKMAEEEQKNHLSGEICAMQTEELAGNSQTLKEPEQVCAQSAILRWKPLPVPFKLLVVSSGNYCSKKQWEPKVHEMSPEAQLTLS

Target ORF information:

RefSeq Version	XM_025279491.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis pericentriolar material 1 (PCM1), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025279491.1

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
AGCAATGAGA GTGTTGATGA CCGACTCAAC AATATGGATT GGGGCAGCCA GCAGAAGAAG 
GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA 
ACTAACGATA TTTCTCCGGA GTCATCACCG GGAGTTGGAA GACGAAGAAC AAAGACTTCA 
CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TCCCCGAGCA GGCAGAACTA 
GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCTCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCCAC TGTAAGTCCT 
CAAAAGAGAG AAATGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAGATTCGTG AGTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGACCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTCACT 
CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT CCAGAAGATT 
CTTGCCAGAG ATCCTCAGCA GGAGCCTATG GAAGAGATAG AAAATTTGAA GAAACAACAT 
GATTTATTAA AAAGGATGTT ACAGCAGCAG GAGCAGCTGA GAGCTCTACA AGGACGACAA 
GCTGCTCTGC TTGCCCTGCA GCATAAAGCT GAGCAGGCTA TAGCTGTGAT GGATGATTCT 
GAAAAGGTTG CGGAGACAAC TCCTGGCCAT CATACTGTAC CAGGCAGACA GCCGGCTCAT 
TCACCTTTGC ATCAGAGAGT ACCCATAAGA GAAACTACAG GTAGCCTATC TGGAGTCAGT 
ATCACCTCTG AACTAAACGA AGAATTGAAT GATTTAATTC AGCATTTTCA TAATCAGCTT 
CGTGATTCTC AGCCTCCAGC TGTTCCAGAC AATAGAAGAC AAGCAGAAAG TCTTTCATTA 
ACTAGGGAGA TTTCTCAGAG CAGAAATCCT TCAGTTTCAG AGCATTTACC TGATGAGAAA 
GTCCAACTTT TTAGCAAGAT GAGAGTGTTA CAAGAAAAGA AACAAAAAAT GGACAAACTT 
CTTGGAGAAC TTCATACACT TCGAGATCAG CATTTGAACA ATTCATCATT TGTGCCTTCT 
TCAGCCTCTC CACAAAGGAG TGTGGATCAG AGAAGTACAA CCACAGCTCC TTCTGCTCCT 
GTAGTCTTAA CACCAGTGGT CAATGGAGAA TCCAATAACC TCACGCCATC TGTTCCTTAT 
CCTGCTGCTT CTCTAGTTTC TCAGAATCAG AATGAAAATG AAGGCCACCT AAATCCAACT 
GAAAAACTCC AGAAGTTAAA TGAAGTTCGA AAGAGGTTGA ATGAACTAAG AGAATTAGTT 
CATTATTATG AGCAAACATC AGATATGATG ACAGATGCTG TGAATGAAAA CACAAAAGAA 
GAAGAAACTG AAGAATCAGA ATATGATTCG GAGCATGAAA ACTCGGAACC TGTTACTAAC 
ATTCGAAATC CACAAGTGGC TGCCACCTGG AACGAAGTAA ATAGTAATTC TAATGCACAG 
TGTGTTTCCA ACAATAGAGA TGGGCGAGCA GTTAATTCTA ACTGTGAAAT TAACAACAGG 
TCTGCTGCCA ACATAAGGGC TCTAAACATG CCTCCTCTTT CAGCAGATTG TCGATACAAT 
AGAGAAGGAG AACAGGAGAT GCCTGTTGCA CAAGGTCATG AGGAGGAGGA GGAGGAGGAG 
GAGGAAGCAG AAGATGAGGC CCTCAGTGGA GCTTCACTGT CTAGTCACAG CAGCAGCCTG 
ATCATTGAGA CTCCTGAAGA TGCTGAATTT GAACATAAGA TCCAGAAGCT TATGGCTGCA 
AAACTGAAAC TTAGACACTT ACAGAACCTT GTAGCCATGG TGCAGGACGA TGATGCAGCC 
AATCAAGGAG TTACTTCTGT GGAGGATTTC TTCCCAGCAG AAGACACCAA ACAAAATGCA 
AATAACACTA GAGGAAATGC CAATAAAACA CAGAAAGATG CTGGAGTAAA TGAAAAGGCA 
AGGGAGAAAT TTTATGAGGC TAAACTACAG CAGCAACAGA GAGAACTCAA ACAATTGCAG 
GAAGAAAGAA AGAAACTGAT TGAGATTCAA GAGAAAATTC AAGAATTGCA AAAGGCATGT 
CCTAACCTAC AGCTGTCAGC TGCTAGTGTG GGTAATTGTC CCACAAAAAC ACATATGCCA 
GCTGTTACTT CATCCCCAAC CGTTAATGAA AATGAAGCCA GTACAAACAA ATCTGGTTTT 
GAGCCTCATG ATTCTTCAGT GGTAGATAAT GAGGTATGGT CAGATATGCG AAGACATGAA 
ATGTTAAGGG AGGAGCTACG ACAGAGAAGA AAGCAGCTGG AAGCTCTGAT GGCTGAACAT 
CAGAGGAGGC AAGGTCTGGC TGAAACTGCA TCTCCAGTGG CTGTTTCACT GAGAAGTGAT 
GGATCTGAGA ACCTGTGTAC ACCTCAGCAA AGCAGAACAG AAAAAACAAT GGCAACTTGG 
GGAGGTTCTA CCCAGTGTGC CCTAGATGAA GAAGGAGATG AAGATGGTTA CCTTTCTGAA 
GGAATTATTC GGACAGACGA AGAGGAGGAA GAGGAGGAGC AAGATGCCAG TTCCAATGAT 
AACTTTCATA TGTATCCTCC AAACAGTGCA AATCATAATT CTTACAATGT AAAGGAAACT 
AAAAATAGGT GGAAGAACAA TCGCCCTTTT TCAGCAGATG GAAATTATCG TCCTTTAGCC 
AAGACAAGAC AGCAGAATAT CAGCATGCAG CGTCAGGAAA ACCTTCGCTG GGTGTCCGAA 
CTCTCTTATG TAGAAGAGAA AGAACAATGG CAAGAACAAA TTAATCAGCT AAAGAAACAA 
CTTGATTTCA GTGTCAATAT TTGTCAGACT TTAATGCAAG ACCAGCAGAC TCTGTCTTGT 
CTGCTACAAA CTCTTCTCAC GAGTCCTTAT AGTGTTATGC CCAGCAGTGT TGCATCCCCG 
CAAGTGCACC TTATAATGCA CCAGTTGAAT CAGTGCTATA CCCAGCTAAC GTGGCAGCAG 
AACAATGTCC AGAGGTTGAA ACAAATGCTC AGTGAACTCA TGCGCCAACA AAATCAGCAT 
CCAGAAAAAC CTGCAAGCAA GGAAGGAGGC AGTGGTACAT CACACCCTCC TTCTCCCAGT 
TTATTTTGTC CTTTCAACTT TCCAACTCAG CCTGTAAATC TGTTTAACCT ACCGGGGTTT 
ACTAATTTTT CGTCATTTGC ACCAAGTATG AATTTCAGCC CTTTATTTCC TTCTAATTTT 
GGAGATTTTT CTCAAAATAT TTCTACACCC ACTGAACAGC AGCAACCGTT AGCCCAGAAT 
CCTTCAGGGA AAACGGAATA CATGGCTTTT CCAAAACCTT TTGAAAGTAG TTCTTCTATT 
GGAGCGGAAA AACAAAGGAA TCAAAAACAG CCTGAAGAGG AGGTGGAGAA CAGTAGGACA 
CCATGGTTTT ATGACCAAGA AGGTGAAGTA GAAAAGCCAT TTCTCAAGAC TGGATTTGCA 
GTGTCTGTAG AGAAAACAAC AAATAGTAAT CGCAAAAATC AACTAGATAC CAGTAGGAGA 
AGACGCCAGT TTGATGAAGT GTCCTTAGAA AGCTTTAACA GTATGCTTGA CCCAGTAGAT 
CCAACAACAG TAACTAAAAC ATTCAAGTCA AGAAAAGCAT CTGCACAGGC CAGCCTGGCA 
TCTAAAGATA AAACTCCCAA ATCAAAGAGT AAGAAGAGGC ATTCTACTCA GCTGAAAAGC 
AGAGTGAAAA ATATCGGGTA TGAAAGTGCC AGTGTGTCTA GCACATGTGA ACCTTGCAAA 
AGTAGAAACA GACATTCAGC CCAAACTGAA GAGCCTGTTC AAGCAAAAGT ACTCAGCAGA 
AAGAATCATG AGCAGCTGGA AAAAGTCATA AGATGTAGTA GGTCTACAGA AATATCTTCA 
GCACATGCTA GGAGAATACT ACAGCAGTCT AACAGAAATG CATGCAATGA AGCGCCAGAA 
ACCGGGAGTG ATTTTTCCAT GTTCGAAGCT TTGCGGGATA CCATTTATTC TGAAGTAGCT 
ACGTTGATTT CTCAAAATGA ATCTCGTCCA CATTTTCTTA TTGAACTCTT CCATGAGCTT 
CAGCTACTTA ATACAGACTA TTTGAGACAG AGGGCCTTAT ATGCATTGCA GGACATAGTA 
TCCAGGCATA TTTCTGAGAA CCATGAAAAA GAAGGAGAAA ATATAAAGTC CGTGAATTCT 
GGTACTTGGA TAGCATCCAA CTCAGAACTT ACTCCCAGTG AAAGCCTTGC TACTACTGAT 
GATGAAACTT TTGAGAAGAA CTTTGAGAGA GAAACACATA AAATAAGTGA GCAAAATGAT 
GCTGATAATG CTAGTGTATC TTCAACTTGT GAGCCTTTTG CAACAGATGT TCTGGGCGAC 
ACTGTGATTC ACTTAGATCA GGCATTAGCC AGAATGAGAG ACTATACGCA TATGAAGATG 
GAGGCTGAAA GTAGTCCAGA CGTCAGGTGC ACCTGCAGGA TCATTGAGGA TGACGATGGT 
GCTGCTGCCA CAACCACGAT TAGTAATAAT TTAGAAGAAA CTCCTGTTAT TGAAAATCAT 
AGTTCACAGC AACCCGTGAG TGAAGTTGCT ACCGTCCCGT GTCCTAGAAT TGATACTCAG 
CAACTGGACC GGCAAATTAA AGCAATTATG AAAGAAGTCA TTCCTTTTTT GAAGGAGCAC 
ATGGATGAAG TATGTTCTTC TCAACTTCTA ACCTCAGTAA GACGCATGGT TTTGGTCCTT 
ACTCAGCAAA ACAATGAGAG CAAAGAGTTT GTAAAGTTCT TTCATAAGCA GCTTGGAAGT 
ATATTACAGG ATTCACTCGC AAAATTTGCT GGCAGAAAAC TGAAGGATTG TGGAGAAGAT 
CTTCTTGTAG AGATATCTGA AGTGTTATTT AATGAATTGG CTTTCTTTAA GCTCATGCAA 
GATTTGGATA ATAATAGTGT AACTGTTAAA CAGAGATGCA AAAGGAAAAT AGAAGCTGCT 
GGAGTGATAC AGTCTTATGC AAAAGAGGCC AAAAGGATTC TTGAAGGAGA TCACAGTTCA 
CCTGCTGGAG AAATTGATGA TGAAGACAAA GACAAGGATG AGACGGAAAC AGTTAAGCAG 
ACTCAAACGT CTGAGGTTTA TGATGGTGAC GGCCCCAAAA ATGTGAGATC TGATGTTTCT 
GATCAAGAGG ATGATGAAGA AAGTGAAGAA TGTCCAGTGT CTATTAATTT GTCCAAAGCT 
GAAACTCAGG CTTTGACTAA TTACGGAAGT GGAGAAGATG AGAATGAAGA TGAAGAAATA 
GAAGAATTTG AAGAGGGACC TGTAGATGTC CAGACGTCAC TTCAGGCTAA CACTGAAACT 
ACAGAAGAAA ATGAACACGA TGATCAGGTT CCACAGCATG ATTTTGAAAA ATCATCAGAA 
AGCAAAAATG TCCCATCAGA ACAAGAACCC ACTACTAGTA AAGACTTTCT TTTGATGACA 
GGTGACCAGA ATAGCACTCC TGTGAAGCCC TGTTACCTCA GTATCGTGGA AAATGAGCAA 
CCTTTAAATA GCCCTGTCCA GAAGGACACT CTCACTACCA TCGACTCATC CAGTCAGCCT 
AACCCTTTGC CTTTACCTTT AACTGAAATT GAAACCTTGG TGCCTAGAGT CAAAGAAGTG 
AAATCTGCTC AGGAGACTCC TGAAAGTTCT TTGGCTGGAA GTCCTGATAC TGAATCTCCA 
GTGTTAGTGA ATGACTATGA AGCAGAATCT GGTAACATAA GTCAAAAATC TGATGAAGAA 
GACTTTGTGA AAGTTGAAGA TTTACCACTT AAACTGACAA TATATTCAGA GGCAGATCTA 
AGGAAGAAAA TGGCCGAAGA AGAACAGAAA AACCACTTAT CTGGTGAAAT ATGTGCAATG 
CAGACTGAAG AATTAGCTGG GAATTCTCAG ACACTAAAAG AACCTGAGCA AGTATGTGCT 
CAGTCTGCCA TCTTAAGATG GAAACCCTTA CCTGTGCCTT TTAAACTGTT GGTGGTGTCA 
TCAGGGAATT ATTGTAGCAA AAAACAGTGG GAGCCCAAAG TACATGAGAT GTCTCCAGAG 
GCTCAGCTAA CTCTTTCTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( XM_025279498.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025135283.1 Bubalus bubalis pericentriolar material 1 (PCM1), transcript variant X17, mRNA.

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CloneID	OBu42305
Clone ID Related Accession (Same CDS sequence)	XM_025279498.1
Accession Version	XM_025279498.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6318bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	pericentriolar material 1 protein isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037545.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG GAGGAGGTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
AGCAATGAGA GTGTTGATGA CCGACTCAAC AATATGGATT GGGGCAGCCA GCAGAAGAAG 
GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA 
ACTAACGATA TTTCTCCGGA GTCATCACCG GGAGTTGGAA GACGAAGAAC AAAGACTTCA 
CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TCCCCGAGCA GGCAGAACTA 
GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCTCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCCAC TGTAAGTCCT 
CAAAAGAGAG AAATGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAGATTCGTG AGTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGACCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTCACT 
CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT CCAGAAGATT 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCGGT CTGAGGCTTC AGATGCCACG 
GAGGCATCTT TTAGTCTGAG AATTCGGCCG TGCGTTGAGG ACAAACTAGG GAATTCAGCT 
TCACAAGCAC AGGTTTCAGA CACTGACGTT ACTACAAGTC CAAAAGGGAA AGGTGACAGA 
CCTCCTCAGC ATGACAGGGA GCTGAGGCCT AATAGGAAAT GTAGCCGAAA ACGTGGATTT 
CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATGGAAG AGATAGAAAA TTTGAAGAAA 
CAACATGATT TATTAAAAAG GATGTTACAG CAGCAGGAGC AGCTGAGAGC TCTACAAGGA 
CGACAAGCTG CTCTGCTTGC CCTGCAGCAT AAAGCTGAGC AGGCTATAGC TGTGATGGAT 
GATTCTGAAA AGGTTGCGGA GACAACTCCT GGCCATCATA CTGTACCAGG CAGACAGCCG 
GCTCATTCAC CTTTGCATCA GAGAGTACCC ATAAGAGAAA CTACAGGTAG CCTATCTGGA 
GTCAGTATCA CCTCTGAACT AAACGAAGAA TTGAATGATT TAATTCAGCA TTTTCATAAT 
CAGCTTCGTG ATTCTCAGCC TCCAGCTGTT CCAGACAATA GAAGACAAGC AGAAAGTCTT 
TCATTAACTA GGGAGATTTC TCAGAGCAGA AATCCTTCAG TTTCAGAGCA TTTACCTGAT 
GAGAAAGTCC AACTTTTTAG CAAGATGAGA GTGTTACAAG AAAAGAAACA AAAAATGGAC 
AAACTTCTTG GAGAACTTCA TACACTTCGA GATCAGCATT TGAACAATTC ATCATTTGTG 
CCTTCTTCAG CCTCTCCACA AAGGAGTGTG GATCAGAGAA GTACAACCAC AGCTCCTTCT 
GCTCCTGTAG TCTTAACACC AGTGGTCAAT GGAGAATCCA ATAACCTCAC GCCATCTGTT 
CCTTATCCTG CTGCTTCTCT AGTTTCTCAG AATCAGAATG AAAATGAAGG CCACCTAAAT 
CCAACTGAAA AACTCCAGAA GTTAAATGAA GTTCGAAAGA GGTTGAATGA ACTAAGAGAA 
TTAGTTCATT ATTATGAGCA AACATCAGAT ATGATGACAG ATGCTGTGAA TGAAAACACA 
AAAGAAGAAG AAACTGAAGA ATCAGAATAT GATTCGGAGC ATGAAAACTC GGAACCTGTT 
ACTAACATTC GAAATCCACA AGTGGCTGCC ACCTGGAACG AAGTAAATAG TAATTCTAAT 
GCACAGTGTG TTTCCAACAA TAGAGATGGG CGAGCAGTTA ATTCTAACTG TGAAATTAAC 
AACAGGTCTG CTGCCAACAT AAGGGCTCTA AACATGCCTC CTCTTTCAGC AGATTGTCGA 
TACAATAGAG AAGGAGAACA GGAGATGCCT GTTGCACAAG GTCATGAGGA GGAGGAGGAG 
GAGGAGGAGG AAGCAGAAGA TGAGGCCCTC AGTGGAGCTT CACTGTCTAG TCACAGCAGC 
AGCCTGATCA TTGAGACTCC TGAAGATGCT GAATTTGAAC ATAAGATCCA GAAGCTTATG 
GCTGCAAAAC TGAAACTTAG ACACTTACAG AACCTTGTAG CCATGGTGCA GGACGATGAT 
GCAGCCAATC AAGGAGTTAC TTCTGTGGAG GATTTCTTCC CAGCAGAAGA CACCAAACAA 
AATGCAAATA ACACTAGAGG AAATGCCAAT AAAACACAGA AAGATGCTGG AGTAAATGAA 
AAGGCAAGGG AGAAATTTTA TGAGGCTAAA CTACAGCAGC AACAGAGAGA ACTCAAACAA 
TTGCAGGAAG AAAGAAAGAA ACTGATTGAG ATTCAAGAGA AAATTCAAGA ATTGCAAAAG 
GCATGTCCTA ACCTACAGCT GTCAGCTGCT AGTGTGGGTA ATTGTCCCAC AAAAACACAT 
ATGCCAGCTG TTACTTCATC CCCAACCGTT AATGAAAATG AAGCCAGTAC AAACAAATCT 
GGTTTTGAGC CTCATGATTC TTCAGTGGTA GATAATGAGG TATGGTCAGA TATGCGAAGA 
CATGAAATGT TAAGGGAGGA GCTACGACAG AGAAGAAAGC AGCTGGAAGC TCTGATGGCT 
GAACATCAGA GGAGGCAAGG TCTGGCTGAA ACTGCATCTC CAGTGGCTGT TTCACTGAGA 
AGTGATGGAT CTGAGAACCT GTGTACACCT CAGCAAAGCA GAACAGAAAA AACAATGGCA 
ACTTGGGGAG GTTCTACCCA GTGTGCCCTA GATGAAGAAG GAGATGAAGA TGGTTACCTT 
TCTGAAGGAA TTATTCGGAC AGACGAAGAG GAGGAAGAGG AGGAGCAAGA TGCCAGTTCC 
AATGATAACT TTCATATGTA TCCTCCAAAC AGTGCAAATC ATAATTCTTA CAATGTAAAG 
GAAACTAAAA ATAGGTGGAA GAACAATCGC CCTTTTTCAG CAGATGGAAA TTATCGTCCT 
TTAGCCAAGA CAAGACAGCA GAATATCAGC ATGCAGCGTC AGGAAAACCT TCGCTGGGTG 
TCCGAACTCT CTTATGTAGA AGAGAAAGAA CAATGGCAAG AACAAATTAA TCAGCTAAAG 
AAACAACTTG ATTTCAGTGT CAATATTTGT CAGACTTTAA TGCAAGACCA GCAGACTCTG 
TCTTGTCTGC TACAAACTCT TCTCACGAGT CCTTATAGTG TTATGCCCAG CAGTGTTGCA 
TCCCCGCAAG TGCACCTTAT AATGCACCAG TTGAATCAGT GCTATACCCA GCTAACGTGG 
CAGCAGAACA ATGTCCAGAG GTTGAAACAA ATGCTCAGTG AACTCATGCG CCAACAAAAT 
CAGCATCCAG AAAAACCTGC AAGCAAGGAA GGAGGCAGTG GTACATCACA CCCTCCTTCT 
CCCAGTTTAT TTTGTCCTTT CAACTTTCCA ACTCAGCCTG TAAATCTGTT TAACCTACCG 
GGGTTTACTA ATTTTTCGTC ATTTGCACCA AGTATGAATT TCAGCCCTTT ATTTCCTTCT 
AATTTTGGAG ATTTTTCTCA AAATATTTCT ACACCCACTG AACAGCAGCA ACCGTTAGCC 
CAGAATCCTT CAGGGAAAAC GGAATACATG GCTTTTCCAA AACCTTTTGA AAGTAGTTCT 
TCTATTGGAG CGGAAAAACA AAGGAATCAA AAACAGCCTG AAGAGGAGGT GGAGAACAGT 
AGGACACCAT GGTTTTATGA CCAAGAAGGT GAAGTAGAAA AGCCATTTCT CAAGACTGGA 
TTTGCAGTGT CTGTAGAGAA AACAACAAAT AGTAATCGCA AAAATCAACT AGATACCAGT 
AGGAGAAGAC GCCAGTTTGA TGAAGTGTCC TTAGAAAGCT TTAACAGTAT GCTTGACCCA 
GTAGATCCAA CAACAGTAAC TAAAACATTC AAGTCAAGAA AAGCATCTGC ACAGGCCAGC 
CTGGCATCTA AAGATAAAAC TCCCAAATCA AAGAGTAAGA AGAGGCATTC TACTCAGCTG 
AAAAGCAGAG TGAAAAATAT CGAAACCGGG AGTGATTTTT CCATGTTCGA AGCTTTGCGG 
GATACCATTT ATTCTGAAGT AGCTACGTTG ATTTCTCAAA ATGAATCTCG TCCACATTTT 
CTTATTGAAC TCTTCCATGA GCTTCAGCTA CTTAATACAG ACTATTTGAG ACAGAGGGCC 
TTATATGCAT TGCAGGACAT AGTATCCAGG CATATTTCTG AGAACCATGA AAAAGAAGGA 
GAAAATATAA AGTCCGTGAA TTCTGGTACT TGGATAGCAT CCAACTCAGA ACTTACTCCC 
AGTGAAAGCC TTGCTACTAC TGATGATGAA ACTTTTGAGA AGAACTTTGA GAGAGAAACA 
CATAAAATAA GTGAGCAAAA TGATGCTGAT AATGCTAGTG TATCTTCAAC TTGTGAGCCT 
TTTGCAACAG ATGTTCTGGG CGACACTGTG ATTCACTTAG ATCAGGCATT AGCCAGAATG 
AGAGACTATA CGCATATGAA GATGGAGGCT GAAAGTAGTC CAGACGTCAG GTGCACCTGC 
AGGATCATTG AGGATGACGA TGGTGCTGCT GCCACAACCA CGATTAGTAA TAATTTAGAA 
GAAACTCCTG TTATTGAAAA TCATAGTTCA CAGCAACCCG TGAGTGAAGT TGCTACCGTC 
CCGTGTCCTA GAATTGATAC TCAGCAACTG GACCGGCAAA TTAAAGCAAT TATGAAAGAA 
GTCATTCCTT TTTTGAAGGA GCACATGGAT GAAGTATGTT CTTCTCAACT TCTAACCTCA 
GTAAGACGCA TGGTTTTGGT CCTTACTCAG CAAAACAATG AGAGCAAAGA GTTTGTAAAG 
TTCTTTCATA AGCAGCTTGG AAGTATATTA CAGGATTCAC TCGCAAAATT TGCTGGCAGA 
AAACTGAAGG ATTGTGGAGA AGATCTTCTT GTAGAGATAT CTGAAGTGTT ATTTAATGAA 
TTGGCTTTCT TTAAGCTCAT GCAAGATTTG GATAATAATA GTGTAACTGT TAAACAGAGA 
TGCAAAAGGA AAATAGAAGC TGCTGGAGTG ATACAGTCTT ATGCAAAAGA GGCCAAAAGG 
ATTCTTGAAG GAGATCACAG TTCACCTGCT GGAGAAATTG ATGATGAAGA CAAAGACAAG 
GATGAGACGG AAACAGTTAA GCAGACTCAA ACGTCTGAGG TTTATGATGG TGACGGCCCC 
AAAAATGTGA GATCTGATGT TTCTGATCAA GAGGATGATG AAGAAAGTGA AGAATGTCCA 
GTGTCTATTA ATTTGTCCAA AGCTGAAACT CAGGCTTTGA CTAATTACGG AAGTGGAGAA 
GATGAGAATG AAGATGAAGA AATAGAAGAA TTTGAAGAGG GACCTGTAGA TGTCCAGACG 
TCACTTCAGG CTAACACTGA AACTACAGAA GAAAATGAAC ACGATGATCA GGTTCCACAG 
CATGATTTTG AAAAATCATC AGAAAGCAAA AATGTCCCAT CAGAACAAGA ACCCACTACT 
AGTAAAGGTG ACCAGAATAG CACTCCTGTG AAGCCCTGTT ACCTCAGTAT CGTGGAAAAT 
GAGCAACCTT TAAATAGCCC TGTCCAGAAG GACACTCTCA CTACCATCGA CTCATCCAGT 
CAGCCTAACC CTTTGCCTTT ACCTTTAACT GAAATTGAAA CCTTGGTGCC TAGAGTCAAA 
GAAGTGAAAT CTGCTCAGGA GACTCCTGAA AGTTCTTTGG CTGGAAGTCC TGATACTGAA 
TCTCCAGTGT TAGTGAATGA CTATGAAGCA GAATCTGGTA ACATAAGTCA AAAATCTGAT 
GAAGAAGACT TTGTGAAAGT TGAAGATTTA CCACTTAAAC TGACAATATA TTCAGAGGCA 
GATCTAAGGA AGAAAATGGC CGAAGAAGAA CAGAAAAACC ACTTATCTGG TGAAATATGT 
GCAATGCAGA CTGAAGAATT AGCTGGGAAT TCTCAGACAC TAAAAGAACC TGAAACAGTG 
GGAGCCCAAA GTACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025135283.1
CDS	392..6709
Translation	MATGGGPFEEGVNDQDLPNWSNESVDDRLNNMDWGSQQKKANRSSEKNKRKFGVESDKRVTNDISPESSPGVGRRRTKTSLTFPHSRYMTQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDATVSPQKREMIGSAQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIREYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLQKILARENEEEDVRTIDSAVGSGSVAESTSLNIDVRSEASDATEASFSLRIRPCVEDKLGNSASQAQVSDTDVTTSPKGKGDRPPQHDRELRPNRKCSRKRGFPSKARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSEKVAETTPGHHTVPGRQPAHSPLHQRVPIRETTGSLSGVSITSELNEELNDLIQHFHNQLRDSQPPAVPDNRRQAESLSLTREISQSRNPSVSEHLPDEKVQLFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSFVPSSASPQRSVDQRSTTTAPSAPVVLTPVVNGESNNLTPSVPYPAASLVSQNQNENEGHLNPTEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENTKEEETEESEYDSEHENSEPVTNIRNPQVAATWNEVNSNSNAQCVSNNRDGRAVNSNCEINNRSAANIRALNMPPLSADCRYNREGEQEMPVAQGHEEEEEEEEEAEDEALSGASLSSHSSSLIIETPEDAEFEHKIQKLMAAKLKLRHLQNLVAMVQDDDAANQGVTSVEDFFPAEDTKQNANNTRGNANKTQKDAGVNEKAREKFYEAKLQQQQRELKQLQEERKKLIEIQEKIQELQKACPNLQLSAASVGNCPTKTHMPAVTSSPTVNENEASTNKSGFEPHDSSVVDNEVWSDMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETASPVAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGIIRTDEEEEEEEQDASSNDNFHMYPPNSANHNSYNVKETKNRWKNNRPFSADGNYRPLAKTRQQNISMQRQENLRWVSELSYVEEKEQWQEQINQLKKQLDFSVNICQTLMQDQQTLSCLLQTLLTSPYSVMPSSVASPQVHLIMHQLNQCYTQLTWQQNNVQRLKQMLSELMRQQNQHPEKPASKEGGSGTSHPPSPSLFCPFNFPTQPVNLFNLPGFTNFSSFAPSMNFSPLFPSNFGDFSQNISTPTEQQQPLAQNPSGKTEYMAFPKPFESSSSIGAEKQRNQKQPEEEVENSRTPWFYDQEGEVEKPFLKTGFAVSVEKTTNSNRKNQLDTSRRRRQFDEVSLESFNSMLDPVDPTTVTKTFKSRKASAQASLASKDKTPKSKSKKRHSTQLKSRVKNIETGSDFSMFEALRDTIYSEVATLISQNESRPHFLIELFHELQLLNTDYLRQRALYALQDIVSRHISENHEKEGENIKSVNSGTWIASNSELTPSESLATTDDETFEKNFERETHKISEQNDADNASVSSTCEPFATDVLGDTVIHLDQALARMRDYTHMKMEAESSPDVRCTCRIIEDDDGAAATTTISNNLEETPVIENHSSQQPVSEVATVPCPRIDTQQLDRQIKAIMKEVIPFLKEHMDEVCSSQLLTSVRRMVLVLTQQNNESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSVTVKQRCKRKIEAAGVIQSYAKEAKRILEGDHSSPAGEIDDEDKDKDETETVKQTQTSEVYDGDGPKNVRSDVSDQEDDEESEECPVSINLSKAETQALTNYGSGEDENEDEEIEEFEEGPVDVQTSLQANTETTEENEHDDQVPQHDFEKSSESKNVPSEQEPTTSKGDQNSTPVKPCYLSIVENEQPLNSPVQKDTLTTIDSSSQPNPLPLPLTEIETLVPRVKEVKSAQETPESSLAGSPDTESPVLVNDYEAESGNISQKSDEEDFVKVEDLPLKLTIYSEADLRKKMAEEEQKNHLSGEICAMQTEELAGNSQTLKEPETVGAQST

Target ORF information:

RefSeq Version	XM_025279498.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis pericentriolar material 1 (PCM1), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025279498.1

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
AGCAATGAGA GTGTTGATGA CCGACTCAAC AATATGGATT GGGGCAGCCA GCAGAAGAAG 
GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA 
ACTAACGATA TTTCTCCGGA GTCATCACCG GGAGTTGGAA GACGAAGAAC AAAGACTTCA 
CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TCCCCGAGCA GGCAGAACTA 
GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCTCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCCAC TGTAAGTCCT 
CAAAAGAGAG AAATGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAGATTCGTG AGTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGACCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTCACT 
CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT CCAGAAGATT 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCGGT CTGAGGCTTC AGATGCCACG 
GAGGCATCTT TTAGTCTGAG AATTCGGCCG TGCGTTGAGG ACAAACTAGG GAATTCAGCT 
TCACAAGCAC AGGTTTCAGA CACTGACGTT ACTACAAGTC CAAAAGGGAA AGGTGACAGA 
CCTCCTCAGC ATGACAGGGA GCTGAGGCCT AATAGGAAAT GTAGCCGAAA ACGTGGATTT 
CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATGGAAG AGATAGAAAA TTTGAAGAAA 
CAACATGATT TATTAAAAAG GATGTTACAG CAGCAGGAGC AGCTGAGAGC TCTACAAGGA 
CGACAAGCTG CTCTGCTTGC CCTGCAGCAT AAAGCTGAGC AGGCTATAGC TGTGATGGAT 
GATTCTGAAA AGGTTGCGGA GACAACTCCT GGCCATCATA CTGTACCAGG CAGACAGCCG 
GCTCATTCAC CTTTGCATCA GAGAGTACCC ATAAGAGAAA CTACAGGTAG CCTATCTGGA 
GTCAGTATCA CCTCTGAACT AAACGAAGAA TTGAATGATT TAATTCAGCA TTTTCATAAT 
CAGCTTCGTG ATTCTCAGCC TCCAGCTGTT CCAGACAATA GAAGACAAGC AGAAAGTCTT 
TCATTAACTA GGGAGATTTC TCAGAGCAGA AATCCTTCAG TTTCAGAGCA TTTACCTGAT 
GAGAAAGTCC AACTTTTTAG CAAGATGAGA GTGTTACAAG AAAAGAAACA AAAAATGGAC 
AAACTTCTTG GAGAACTTCA TACACTTCGA GATCAGCATT TGAACAATTC ATCATTTGTG 
CCTTCTTCAG CCTCTCCACA AAGGAGTGTG GATCAGAGAA GTACAACCAC AGCTCCTTCT 
GCTCCTGTAG TCTTAACACC AGTGGTCAAT GGAGAATCCA ATAACCTCAC GCCATCTGTT 
CCTTATCCTG CTGCTTCTCT AGTTTCTCAG AATCAGAATG AAAATGAAGG CCACCTAAAT 
CCAACTGAAA AACTCCAGAA GTTAAATGAA GTTCGAAAGA GGTTGAATGA ACTAAGAGAA 
TTAGTTCATT ATTATGAGCA AACATCAGAT ATGATGACAG ATGCTGTGAA TGAAAACACA 
AAAGAAGAAG AAACTGAAGA ATCAGAATAT GATTCGGAGC ATGAAAACTC GGAACCTGTT 
ACTAACATTC GAAATCCACA AGTGGCTGCC ACCTGGAACG AAGTAAATAG TAATTCTAAT 
GCACAGTGTG TTTCCAACAA TAGAGATGGG CGAGCAGTTA ATTCTAACTG TGAAATTAAC 
AACAGGTCTG CTGCCAACAT AAGGGCTCTA AACATGCCTC CTCTTTCAGC AGATTGTCGA 
TACAATAGAG AAGGAGAACA GGAGATGCCT GTTGCACAAG GTCATGAGGA GGAGGAGGAG 
GAGGAGGAGG AAGCAGAAGA TGAGGCCCTC AGTGGAGCTT CACTGTCTAG TCACAGCAGC 
AGCCTGATCA TTGAGACTCC TGAAGATGCT GAATTTGAAC ATAAGATCCA GAAGCTTATG 
GCTGCAAAAC TGAAACTTAG ACACTTACAG AACCTTGTAG CCATGGTGCA GGACGATGAT 
GCAGCCAATC AAGGAGTTAC TTCTGTGGAG GATTTCTTCC CAGCAGAAGA CACCAAACAA 
AATGCAAATA ACACTAGAGG AAATGCCAAT AAAACACAGA AAGATGCTGG AGTAAATGAA 
AAGGCAAGGG AGAAATTTTA TGAGGCTAAA CTACAGCAGC AACAGAGAGA ACTCAAACAA 
TTGCAGGAAG AAAGAAAGAA ACTGATTGAG ATTCAAGAGA AAATTCAAGA ATTGCAAAAG 
GCATGTCCTA ACCTACAGCT GTCAGCTGCT AGTGTGGGTA ATTGTCCCAC AAAAACACAT 
ATGCCAGCTG TTACTTCATC CCCAACCGTT AATGAAAATG AAGCCAGTAC AAACAAATCT 
GGTTTTGAGC CTCATGATTC TTCAGTGGTA GATAATGAGG TATGGTCAGA TATGCGAAGA 
CATGAAATGT TAAGGGAGGA GCTACGACAG AGAAGAAAGC AGCTGGAAGC TCTGATGGCT 
GAACATCAGA GGAGGCAAGG TCTGGCTGAA ACTGCATCTC CAGTGGCTGT TTCACTGAGA 
AGTGATGGAT CTGAGAACCT GTGTACACCT CAGCAAAGCA GAACAGAAAA AACAATGGCA 
ACTTGGGGAG GTTCTACCCA GTGTGCCCTA GATGAAGAAG GAGATGAAGA TGGTTACCTT 
TCTGAAGGAA TTATTCGGAC AGACGAAGAG GAGGAAGAGG AGGAGCAAGA TGCCAGTTCC 
AATGATAACT TTCATATGTA TCCTCCAAAC AGTGCAAATC ATAATTCTTA CAATGTAAAG 
GAAACTAAAA ATAGGTGGAA GAACAATCGC CCTTTTTCAG CAGATGGAAA TTATCGTCCT 
TTAGCCAAGA CAAGACAGCA GAATATCAGC ATGCAGCGTC AGGAAAACCT TCGCTGGGTG 
TCCGAACTCT CTTATGTAGA AGAGAAAGAA CAATGGCAAG AACAAATTAA TCAGCTAAAG 
AAACAACTTG ATTTCAGTGT CAATATTTGT CAGACTTTAA TGCAAGACCA GCAGACTCTG 
TCTTGTCTGC TACAAACTCT TCTCACGAGT CCTTATAGTG TTATGCCCAG CAGTGTTGCA 
TCCCCGCAAG TGCACCTTAT AATGCACCAG TTGAATCAGT GCTATACCCA GCTAACGTGG 
CAGCAGAACA ATGTCCAGAG GTTGAAACAA ATGCTCAGTG AACTCATGCG CCAACAAAAT 
CAGCATCCAG AAAAACCTGC AAGCAAGGAA GGAGGCAGTG GTACATCACA CCCTCCTTCT 
CCCAGTTTAT TTTGTCCTTT CAACTTTCCA ACTCAGCCTG TAAATCTGTT TAACCTACCG 
GGGTTTACTA ATTTTTCGTC ATTTGCACCA AGTATGAATT TCAGCCCTTT ATTTCCTTCT 
AATTTTGGAG ATTTTTCTCA AAATATTTCT ACACCCACTG AACAGCAGCA ACCGTTAGCC 
CAGAATCCTT CAGGGAAAAC GGAATACATG GCTTTTCCAA AACCTTTTGA AAGTAGTTCT 
TCTATTGGAG CGGAAAAACA AAGGAATCAA AAACAGCCTG AAGAGGAGGT GGAGAACAGT 
AGGACACCAT GGTTTTATGA CCAAGAAGGT GAAGTAGAAA AGCCATTTCT CAAGACTGGA 
TTTGCAGTGT CTGTAGAGAA AACAACAAAT AGTAATCGCA AAAATCAACT AGATACCAGT 
AGGAGAAGAC GCCAGTTTGA TGAAGTGTCC TTAGAAAGCT TTAACAGTAT GCTTGACCCA 
GTAGATCCAA CAACAGTAAC TAAAACATTC AAGTCAAGAA AAGCATCTGC ACAGGCCAGC 
CTGGCATCTA AAGATAAAAC TCCCAAATCA AAGAGTAAGA AGAGGCATTC TACTCAGCTG 
AAAAGCAGAG TGAAAAATAT CGAAACCGGG AGTGATTTTT CCATGTTCGA AGCTTTGCGG 
GATACCATTT ATTCTGAAGT AGCTACGTTG ATTTCTCAAA ATGAATCTCG TCCACATTTT 
CTTATTGAAC TCTTCCATGA GCTTCAGCTA CTTAATACAG ACTATTTGAG ACAGAGGGCC 
TTATATGCAT TGCAGGACAT AGTATCCAGG CATATTTCTG AGAACCATGA AAAAGAAGGA 
GAAAATATAA AGTCCGTGAA TTCTGGTACT TGGATAGCAT CCAACTCAGA ACTTACTCCC 
AGTGAAAGCC TTGCTACTAC TGATGATGAA ACTTTTGAGA AGAACTTTGA GAGAGAAACA 
CATAAAATAA GTGAGCAAAA TGATGCTGAT AATGCTAGTG TATCTTCAAC TTGTGAGCCT 
TTTGCAACAG ATGTTCTGGG CGACACTGTG ATTCACTTAG ATCAGGCATT AGCCAGAATG 
AGAGACTATA CGCATATGAA GATGGAGGCT GAAAGTAGTC CAGACGTCAG GTGCACCTGC 
AGGATCATTG AGGATGACGA TGGTGCTGCT GCCACAACCA CGATTAGTAA TAATTTAGAA 
GAAACTCCTG TTATTGAAAA TCATAGTTCA CAGCAACCCG TGAGTGAAGT TGCTACCGTC 
CCGTGTCCTA GAATTGATAC TCAGCAACTG GACCGGCAAA TTAAAGCAAT TATGAAAGAA 
GTCATTCCTT TTTTGAAGGA GCACATGGAT GAAGTATGTT CTTCTCAACT TCTAACCTCA 
GTAAGACGCA TGGTTTTGGT CCTTACTCAG CAAAACAATG AGAGCAAAGA GTTTGTAAAG 
TTCTTTCATA AGCAGCTTGG AAGTATATTA CAGGATTCAC TCGCAAAATT TGCTGGCAGA 
AAACTGAAGG ATTGTGGAGA AGATCTTCTT GTAGAGATAT CTGAAGTGTT ATTTAATGAA 
TTGGCTTTCT TTAAGCTCAT GCAAGATTTG GATAATAATA GTGTAACTGT TAAACAGAGA 
TGCAAAAGGA AAATAGAAGC TGCTGGAGTG ATACAGTCTT ATGCAAAAGA GGCCAAAAGG 
ATTCTTGAAG GAGATCACAG TTCACCTGCT GGAGAAATTG ATGATGAAGA CAAAGACAAG 
GATGAGACGG AAACAGTTAA GCAGACTCAA ACGTCTGAGG TTTATGATGG TGACGGCCCC 
AAAAATGTGA GATCTGATGT TTCTGATCAA GAGGATGATG AAGAAAGTGA AGAATGTCCA 
GTGTCTATTA ATTTGTCCAA AGCTGAAACT CAGGCTTTGA CTAATTACGG AAGTGGAGAA 
GATGAGAATG AAGATGAAGA AATAGAAGAA TTTGAAGAGG GACCTGTAGA TGTCCAGACG 
TCACTTCAGG CTAACACTGA AACTACAGAA GAAAATGAAC ACGATGATCA GGTTCCACAG 
CATGATTTTG AAAAATCATC AGAAAGCAAA AATGTCCCAT CAGAACAAGA ACCCACTACT 
AGTAAAGGTG ACCAGAATAG CACTCCTGTG AAGCCCTGTT ACCTCAGTAT CGTGGAAAAT 
GAGCAACCTT TAAATAGCCC TGTCCAGAAG GACACTCTCA CTACCATCGA CTCATCCAGT 
CAGCCTAACC CTTTGCCTTT ACCTTTAACT GAAATTGAAA CCTTGGTGCC TAGAGTCAAA 
GAAGTGAAAT CTGCTCAGGA GACTCCTGAA AGTTCTTTGG CTGGAAGTCC TGATACTGAA 
TCTCCAGTGT TAGTGAATGA CTATGAAGCA GAATCTGGTA ACATAAGTCA AAAATCTGAT 
GAAGAAGACT TTGTGAAAGT TGAAGATTTA CCACTTAAAC TGACAATATA TTCAGAGGCA 
GATCTAAGGA AGAAAATGGC CGAAGAAGAA CAGAAAAACC ACTTATCTGG TGAAATATGT 
GCAATGCAGA CTGAAGAATT AGCTGGGAAT TCTCAGACAC TAAAAGAACC TGAAACAGTG 
GGAGCCCAAA GTACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( XM_025279504.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025135289.1 Bubalus bubalis pericentriolar material 1 (PCM1), transcript variant X18, mRNA.

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CloneID	OBu42306
Clone ID Related Accession (Same CDS sequence)	XM_025279504.1
Accession Version	XM_025279504.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6315bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	pericentriolar material 1 protein isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037545.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG GAGGAGGTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
AGCAATGAGA GTGTTGATGA CCGACTCAAC AATATGGATT GGGGCAGCCA GCAGAAGAAG 
GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA 
ACTAACGATA TTTCTCCGGA GTCATCACCG GGAGTTGGAA GACGAAGAAC AAAGACTTCA 
CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TCCCCGAGCA GGCAGAACTA 
GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCTCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCCAC TGTAAGTCCT 
CAAAAGAGAG AAATGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAGATTCGTG AGTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGACCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTCACT 
CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT CCAGAAGATT 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCGGT CTGAGGCTTC AGATGCCACG 
GAGGCATCTT TTAGTCTGAG AATTCGGCCG TGCGTTGAGG ACAAACTAGG GAATTCAGCT 
TCACAAGCAC AGGTTTCAGA CACTGACGTT ACTACAAGTC CAAAAGGGAA AGGTGACAGA 
CCTCCTCAGC ATGACAGGGA GCTGAGGCCT AATAGGAAAT GTAGCCGAAA ACGTGGATTT 
CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATGGAAG AGATAGAAAA TTTGAAGAAA 
CAACATGATT TATTAAAAAG GATGTTACAG CAGCAGGAGC AGCTGAGAGC TCTACAAGGA 
CGACAAGCTG CTCTGCTTGC CCTGCAGCAT AAAGCTGAGC AGGCTATAGC TGTGATGGAT 
GATTCTGAAA AGGTTGCGGA GACAACTCCT GGCCATCATA CTGTACCAGG CAGACAGCCG 
GCTCATTCAC CTTTGCATCA GAGAGTACCC ATAAGAGAAA CTACAGGTAG CCTATCTGGA 
GTCAGTATCA CCTCTGAACT AAACGAAGAA TTGAATGATT TAATTCAGCA TTTTCATAAT 
CAGCTTCGTG ATTCTCAGCC TCCAGCTGTT CCAGACAATA GAAGACAAGC AGAAAGTCTT 
TCATTAACTA GGGAGATTTC TCAGAGCAGA AATCCTTCAG TTTCAGAGCA TTTACCTGAT 
GAGAAAGTCC AACTTTTTAG CAAGATGAGA GTGTTACAAG AAAAGAAACA AAAAATGGAC 
AAACTTCTTG GAGAACTTCA TACACTTCGA GATCAGCATT TGAACAATTC ATCATTTGTG 
CCTTCTTCAG CCTCTCCACA AAGGAGTGTG GATCAGAGAA GTACAACCAC AGCTCCTTCT 
GCTCCTGTAG TCTTAACACC AGTGGTCAAT GGAGAATCCA ATAACCTCAC GCCATCTGTT 
CCTTATCCTG CTGCTTCTCT AGTTTCTCAG AATCAGAATG AAAATGAAGG CCACCTAAAT 
CCAACTGAAA AACTCCAGAA GTTAAATGAA GTTCGAAAGA GGTTGAATGA ACTAAGAGAA 
TTAGTTCATT ATTATGAGCA AACATCAGAT ATGATGACAG ATGCTGTGAA TGAAAACACA 
AAAGAAGAAG AAACTGAAGA ATCAGAATAT GATTCGGAGC ATGAAAACTC GGAACCTGTT 
ACTAACATTC GAAATCCACA AGTGGCTGCC ACCTGGAACG AAGTAAATAG TAATTCTAAT 
GCACAGTGTG TTTCCAACAA TAGAGATGGG CGAGCAGTTA ATTCTAACTG TGAAATTAAC 
AACAGGTCTG CTGCCAACAT AAGGGCTCTA AACATGCCTC CTCTTTCAGA TTGTCGATAC 
AATAGAGAAG GAGAACAGGA GATGCCTGTT GCACAAGGTC ATGAGGAGGA GGAGGAGGAG 
GAGGAGGAAG CAGAAGATGA GGCCCTCAGT GGAGCTTCAC TGTCTAGTCA CAGCAGCAGC 
CTGATCATTG AGACTCCTGA AGATGCTGAA TTTGAACATA AGATCCAGAA GCTTATGGCT 
GCAAAACTGA AACTTAGACA CTTACAGAAC CTTGTAGCCA TGGTGCAGGA CGATGATGCA 
GCCAATCAAG GAGTTACTTC TGTGGAGGAT TTCTTCCCAG CAGAAGACAC CAAACAAAAT 
GCAAATAACA CTAGAGGAAA TGCCAATAAA ACACAGAAAG ATGCTGGAGT AAATGAAAAG 
GCAAGGGAGA AATTTTATGA GGCTAAACTA CAGCAGCAAC AGAGAGAACT CAAACAATTG 
CAGGAAGAAA GAAAGAAACT GATTGAGATT CAAGAGAAAA TTCAAGAATT GCAAAAGGCA 
TGTCCTAACC TACAGCTGTC AGCTGCTAGT GTGGGTAATT GTCCCACAAA AACACATATG 
CCAGCTGTTA CTTCATCCCC AACCGTTAAT GAAAATGAAG CCAGTACAAA CAAATCTGGT 
TTTGAGCCTC ATGATTCTTC AGTGGTAGAT AATGAGGTAT GGTCAGATAT GCGAAGACAT 
GAAATGTTAA GGGAGGAGCT ACGACAGAGA AGAAAGCAGC TGGAAGCTCT GATGGCTGAA 
CATCAGAGGA GGCAAGGTCT GGCTGAAACT GCATCTCCAG TGGCTGTTTC ACTGAGAAGT 
GATGGATCTG AGAACCTGTG TACACCTCAG CAAAGCAGAA CAGAAAAAAC AATGGCAACT 
TGGGGAGGTT CTACCCAGTG TGCCCTAGAT GAAGAAGGAG ATGAAGATGG TTACCTTTCT 
GAAGGAATTA TTCGGACAGA CGAAGAGGAG GAAGAGGAGG AGCAAGATGC CAGTTCCAAT 
GATAACTTTC ATATGTATCC TCCAAACAGT GCAAATCATA ATTCTTACAA TGTAAAGGAA 
ACTAAAAATA GGTGGAAGAA CAATCGCCCT TTTTCAGCAG ATGGAAATTA TCGTCCTTTA 
GCCAAGACAA GACAGCAGAA TATCAGCATG CAGCGTCAGG AAAACCTTCG CTGGGTGTCC 
GAACTCTCTT ATGTAGAAGA GAAAGAACAA TGGCAAGAAC AAATTAATCA GCTAAAGAAA 
CAACTTGATT TCAGTGTCAA TATTTGTCAG ACTTTAATGC AAGACCAGCA GACTCTGTCT 
TGTCTGCTAC AAACTCTTCT CACGAGTCCT TATAGTGTTA TGCCCAGCAG TGTTGCATCC 
CCGCAAGTGC ACCTTATAAT GCACCAGTTG AATCAGTGCT ATACCCAGCT AACGTGGCAG 
CAGAACAATG TCCAGAGGTT GAAACAAATG CTCAGTGAAC TCATGCGCCA ACAAAATCAG 
CATCCAGAAA AACCTGCAAG CAAGGAAGGA GGCAGTGGTA CATCACACCC TCCTTCTCCC 
AGTTTATTTT GTCCTTTCAA CTTTCCAACT CAGCCTGTAA ATCTGTTTAA CCTACCGGGG 
TTTACTAATT TTTCGTCATT TGCACCAAGT ATGAATTTCA GCCCTTTATT TCCTTCTAAT 
TTTGGAGATT TTTCTCAAAA TATTTCTACA CCCACTGAAC AGCAGCAACC GTTAGCCCAG 
AATCCTTCAG GGAAAACGGA ATACATGGCT TTTCCAAAAC CTTTTGAAAG TAGTTCTTCT 
ATTGGAGCGG AAAAACAAAG GAATCAAAAA CAGCCTGAAG AGGAGGTGGA GAACAGTAGG 
ACACCATGGT TTTATGACCA AGAAGGTGAA GTAGAAAAGC CATTTCTCAA GACTGGATTT 
GCAGTGTCTG TAGAGAAAAC AACAAATAGT AATCGCAAAA ATCAACTAGA TACCAGTAGG 
AGAAGACGCC AGTTTGATGA AGTGTCCTTA GAAAGCTTTA ACAGTATGCT TGACCCAGTA 
GATCCAACAA CAGTAACTAA AACATTCAAG TCAAGAAAAG CATCTGCACA GGCCAGCCTG 
GCATCTAAAG ATAAAACTCC CAAATCAAAG AGTAAGAAGA GGCATTCTAC TCAGCTGAAA 
AGCAGAGTGA AAAATATCGA AACCGGGAGT GATTTTTCCA TGTTCGAAGC TTTGCGGGAT 
ACCATTTATT CTGAAGTAGC TACGTTGATT TCTCAAAATG AATCTCGTCC ACATTTTCTT 
ATTGAACTCT TCCATGAGCT TCAGCTACTT AATACAGACT ATTTGAGACA GAGGGCCTTA 
TATGCATTGC AGGACATAGT ATCCAGGCAT ATTTCTGAGA ACCATGAAAA AGAAGGAGAA 
AATATAAAGT CCGTGAATTC TGGTACTTGG ATAGCATCCA ACTCAGAACT TACTCCCAGT 
GAAAGCCTTG CTACTACTGA TGATGAAACT TTTGAGAAGA ACTTTGAGAG AGAAACACAT 
AAAATAAGTG AGCAAAATGA TGCTGATAAT GCTAGTGTAT CTTCAACTTG TGAGCCTTTT 
GCAACAGATG TTCTGGGCGA CACTGTGATT CACTTAGATC AGGCATTAGC CAGAATGAGA 
GACTATACGC ATATGAAGAT GGAGGCTGAA AGTAGTCCAG ACGTCAGGTG CACCTGCAGG 
ATCATTGAGG ATGACGATGG TGCTGCTGCC ACAACCACGA TTAGTAATAA TTTAGAAGAA 
ACTCCTGTTA TTGAAAATCA TAGTTCACAG CAACCCGTGA GTGAAGTTGC TACCGTCCCG 
TGTCCTAGAA TTGATACTCA GCAACTGGAC CGGCAAATTA AAGCAATTAT GAAAGAAGTC 
ATTCCTTTTT TGAAGGAGCA CATGGATGAA GTATGTTCTT CTCAACTTCT AACCTCAGTA 
AGACGCATGG TTTTGGTCCT TACTCAGCAA AACAATGAGA GCAAAGAGTT TGTAAAGTTC 
TTTCATAAGC AGCTTGGAAG TATATTACAG GATTCACTCG CAAAATTTGC TGGCAGAAAA 
CTGAAGGATT GTGGAGAAGA TCTTCTTGTA GAGATATCTG AAGTGTTATT TAATGAATTG 
GCTTTCTTTA AGCTCATGCA AGATTTGGAT AATAATAGTG TAACTGTTAA ACAGAGATGC 
AAAAGGAAAA TAGAAGCTGC TGGAGTGATA CAGTCTTATG CAAAAGAGGC CAAAAGGATT 
CTTGAAGGAG ATCACAGTTC ACCTGCTGGA GAAATTGATG ATGAAGACAA AGACAAGGAT 
GAGACGGAAA CAGTTAAGCA GACTCAAACG TCTGAGGTTT ATGATGGTGA CGGCCCCAAA 
AATGTGAGAT CTGATGTTTC TGATCAAGAG GATGATGAAG AAAGTGAAGA ATGTCCAGTG 
TCTATTAATT TGTCCAAAGC TGAAACTCAG GCTTTGACTA ATTACGGAAG TGGAGAAGAT 
GAGAATGAAG ATGAAGAAAT AGAAGAATTT GAAGAGGGAC CTGTAGATGT CCAGACGTCA 
CTTCAGGCTA ACACTGAAAC TACAGAAGAA AATGAACACG ATGATCAGGT TCCACAGCAT 
GATTTTGAAA AATCATCAGA AAGCAAAAAT GTCCCATCAG AACAAGAACC CACTACTAGT 
AAAGGTGACC AGAATAGCAC TCCTGTGAAG CCCTGTTACC TCAGTATCGT GGAAAATGAG 
CAACCTTTAA ATAGCCCTGT CCAGAAGGAC ACTCTCACTA CCATCGACTC ATCCAGTCAG 
CCTAACCCTT TGCCTTTACC TTTAACTGAA ATTGAAACCT TGGTGCCTAG AGTCAAAGAA 
GTGAAATCTG CTCAGGAGAC TCCTGAAAGT TCTTTGGCTG GAAGTCCTGA TACTGAATCT 
CCAGTGTTAG TGAATGACTA TGAAGCAGAA TCTGGTAACA TAAGTCAAAA ATCTGATGAA 
GAAGACTTTG TGAAAGTTGA AGATTTACCA CTTAAACTGA CAATATATTC AGAGGCAGAT 
CTAAGGAAGA AAATGGCCGA AGAAGAACAG AAAAACCACT TATCTGGTGA AATATGTGCA 
ATGCAGACTG AAGAATTAGC TGGGAATTCT CAGACACTAA AAGAACCTGA AACAGTGGGA 
GCCCAAAGTA CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025135289.1
CDS	392..6706
Translation	MATGGGPFEEGVNDQDLPNWSNESVDDRLNNMDWGSQQKKANRSSEKNKRKFGVESDKRVTNDISPESSPGVGRRRTKTSLTFPHSRYMTQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDATVSPQKREMIGSAQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIREYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLQKILARENEEEDVRTIDSAVGSGSVAESTSLNIDVRSEASDATEASFSLRIRPCVEDKLGNSASQAQVSDTDVTTSPKGKGDRPPQHDRELRPNRKCSRKRGFPSKARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSEKVAETTPGHHTVPGRQPAHSPLHQRVPIRETTGSLSGVSITSELNEELNDLIQHFHNQLRDSQPPAVPDNRRQAESLSLTREISQSRNPSVSEHLPDEKVQLFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSFVPSSASPQRSVDQRSTTTAPSAPVVLTPVVNGESNNLTPSVPYPAASLVSQNQNENEGHLNPTEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENTKEEETEESEYDSEHENSEPVTNIRNPQVAATWNEVNSNSNAQCVSNNRDGRAVNSNCEINNRSAANIRALNMPPLSDCRYNREGEQEMPVAQGHEEEEEEEEEAEDEALSGASLSSHSSSLIIETPEDAEFEHKIQKLMAAKLKLRHLQNLVAMVQDDDAANQGVTSVEDFFPAEDTKQNANNTRGNANKTQKDAGVNEKAREKFYEAKLQQQQRELKQLQEERKKLIEIQEKIQELQKACPNLQLSAASVGNCPTKTHMPAVTSSPTVNENEASTNKSGFEPHDSSVVDNEVWSDMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETASPVAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGIIRTDEEEEEEEQDASSNDNFHMYPPNSANHNSYNVKETKNRWKNNRPFSADGNYRPLAKTRQQNISMQRQENLRWVSELSYVEEKEQWQEQINQLKKQLDFSVNICQTLMQDQQTLSCLLQTLLTSPYSVMPSSVASPQVHLIMHQLNQCYTQLTWQQNNVQRLKQMLSELMRQQNQHPEKPASKEGGSGTSHPPSPSLFCPFNFPTQPVNLFNLPGFTNFSSFAPSMNFSPLFPSNFGDFSQNISTPTEQQQPLAQNPSGKTEYMAFPKPFESSSSIGAEKQRNQKQPEEEVENSRTPWFYDQEGEVEKPFLKTGFAVSVEKTTNSNRKNQLDTSRRRRQFDEVSLESFNSMLDPVDPTTVTKTFKSRKASAQASLASKDKTPKSKSKKRHSTQLKSRVKNIETGSDFSMFEALRDTIYSEVATLISQNESRPHFLIELFHELQLLNTDYLRQRALYALQDIVSRHISENHEKEGENIKSVNSGTWIASNSELTPSESLATTDDETFEKNFERETHKISEQNDADNASVSSTCEPFATDVLGDTVIHLDQALARMRDYTHMKMEAESSPDVRCTCRIIEDDDGAAATTTISNNLEETPVIENHSSQQPVSEVATVPCPRIDTQQLDRQIKAIMKEVIPFLKEHMDEVCSSQLLTSVRRMVLVLTQQNNESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSVTVKQRCKRKIEAAGVIQSYAKEAKRILEGDHSSPAGEIDDEDKDKDETETVKQTQTSEVYDGDGPKNVRSDVSDQEDDEESEECPVSINLSKAETQALTNYGSGEDENEDEEIEEFEEGPVDVQTSLQANTETTEENEHDDQVPQHDFEKSSESKNVPSEQEPTTSKGDQNSTPVKPCYLSIVENEQPLNSPVQKDTLTTIDSSSQPNPLPLPLTEIETLVPRVKEVKSAQETPESSLAGSPDTESPVLVNDYEAESGNISQKSDEEDFVKVEDLPLKLTIYSEADLRKKMAEEEQKNHLSGEICAMQTEELAGNSQTLKEPETVGAQST

Target ORF information:

RefSeq Version	XM_025279504.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis pericentriolar material 1 (PCM1), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025279504.1

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
AGCAATGAGA GTGTTGATGA CCGACTCAAC AATATGGATT GGGGCAGCCA GCAGAAGAAG 
GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA 
ACTAACGATA TTTCTCCGGA GTCATCACCG GGAGTTGGAA GACGAAGAAC AAAGACTTCA 
CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TCCCCGAGCA GGCAGAACTA 
GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCTCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCCAC TGTAAGTCCT 
CAAAAGAGAG AAATGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAGATTCGTG AGTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGACCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTCACT 
CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT CCAGAAGATT 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCGGT CTGAGGCTTC AGATGCCACG 
GAGGCATCTT TTAGTCTGAG AATTCGGCCG TGCGTTGAGG ACAAACTAGG GAATTCAGCT 
TCACAAGCAC AGGTTTCAGA CACTGACGTT ACTACAAGTC CAAAAGGGAA AGGTGACAGA 
CCTCCTCAGC ATGACAGGGA GCTGAGGCCT AATAGGAAAT GTAGCCGAAA ACGTGGATTT 
CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATGGAAG AGATAGAAAA TTTGAAGAAA 
CAACATGATT TATTAAAAAG GATGTTACAG CAGCAGGAGC AGCTGAGAGC TCTACAAGGA 
CGACAAGCTG CTCTGCTTGC CCTGCAGCAT AAAGCTGAGC AGGCTATAGC TGTGATGGAT 
GATTCTGAAA AGGTTGCGGA GACAACTCCT GGCCATCATA CTGTACCAGG CAGACAGCCG 
GCTCATTCAC CTTTGCATCA GAGAGTACCC ATAAGAGAAA CTACAGGTAG CCTATCTGGA 
GTCAGTATCA CCTCTGAACT AAACGAAGAA TTGAATGATT TAATTCAGCA TTTTCATAAT 
CAGCTTCGTG ATTCTCAGCC TCCAGCTGTT CCAGACAATA GAAGACAAGC AGAAAGTCTT 
TCATTAACTA GGGAGATTTC TCAGAGCAGA AATCCTTCAG TTTCAGAGCA TTTACCTGAT 
GAGAAAGTCC AACTTTTTAG CAAGATGAGA GTGTTACAAG AAAAGAAACA AAAAATGGAC 
AAACTTCTTG GAGAACTTCA TACACTTCGA GATCAGCATT TGAACAATTC ATCATTTGTG 
CCTTCTTCAG CCTCTCCACA AAGGAGTGTG GATCAGAGAA GTACAACCAC AGCTCCTTCT 
GCTCCTGTAG TCTTAACACC AGTGGTCAAT GGAGAATCCA ATAACCTCAC GCCATCTGTT 
CCTTATCCTG CTGCTTCTCT AGTTTCTCAG AATCAGAATG AAAATGAAGG CCACCTAAAT 
CCAACTGAAA AACTCCAGAA GTTAAATGAA GTTCGAAAGA GGTTGAATGA ACTAAGAGAA 
TTAGTTCATT ATTATGAGCA AACATCAGAT ATGATGACAG ATGCTGTGAA TGAAAACACA 
AAAGAAGAAG AAACTGAAGA ATCAGAATAT GATTCGGAGC ATGAAAACTC GGAACCTGTT 
ACTAACATTC GAAATCCACA AGTGGCTGCC ACCTGGAACG AAGTAAATAG TAATTCTAAT 
GCACAGTGTG TTTCCAACAA TAGAGATGGG CGAGCAGTTA ATTCTAACTG TGAAATTAAC 
AACAGGTCTG CTGCCAACAT AAGGGCTCTA AACATGCCTC CTCTTTCAGA TTGTCGATAC 
AATAGAGAAG GAGAACAGGA GATGCCTGTT GCACAAGGTC ATGAGGAGGA GGAGGAGGAG 
GAGGAGGAAG CAGAAGATGA GGCCCTCAGT GGAGCTTCAC TGTCTAGTCA CAGCAGCAGC 
CTGATCATTG AGACTCCTGA AGATGCTGAA TTTGAACATA AGATCCAGAA GCTTATGGCT 
GCAAAACTGA AACTTAGACA CTTACAGAAC CTTGTAGCCA TGGTGCAGGA CGATGATGCA 
GCCAATCAAG GAGTTACTTC TGTGGAGGAT TTCTTCCCAG CAGAAGACAC CAAACAAAAT 
GCAAATAACA CTAGAGGAAA TGCCAATAAA ACACAGAAAG ATGCTGGAGT AAATGAAAAG 
GCAAGGGAGA AATTTTATGA GGCTAAACTA CAGCAGCAAC AGAGAGAACT CAAACAATTG 
CAGGAAGAAA GAAAGAAACT GATTGAGATT CAAGAGAAAA TTCAAGAATT GCAAAAGGCA 
TGTCCTAACC TACAGCTGTC AGCTGCTAGT GTGGGTAATT GTCCCACAAA AACACATATG 
CCAGCTGTTA CTTCATCCCC AACCGTTAAT GAAAATGAAG CCAGTACAAA CAAATCTGGT 
TTTGAGCCTC ATGATTCTTC AGTGGTAGAT AATGAGGTAT GGTCAGATAT GCGAAGACAT 
GAAATGTTAA GGGAGGAGCT ACGACAGAGA AGAAAGCAGC TGGAAGCTCT GATGGCTGAA 
CATCAGAGGA GGCAAGGTCT GGCTGAAACT GCATCTCCAG TGGCTGTTTC ACTGAGAAGT 
GATGGATCTG AGAACCTGTG TACACCTCAG CAAAGCAGAA CAGAAAAAAC AATGGCAACT 
TGGGGAGGTT CTACCCAGTG TGCCCTAGAT GAAGAAGGAG ATGAAGATGG TTACCTTTCT 
GAAGGAATTA TTCGGACAGA CGAAGAGGAG GAAGAGGAGG AGCAAGATGC CAGTTCCAAT 
GATAACTTTC ATATGTATCC TCCAAACAGT GCAAATCATA ATTCTTACAA TGTAAAGGAA 
ACTAAAAATA GGTGGAAGAA CAATCGCCCT TTTTCAGCAG ATGGAAATTA TCGTCCTTTA 
GCCAAGACAA GACAGCAGAA TATCAGCATG CAGCGTCAGG AAAACCTTCG CTGGGTGTCC 
GAACTCTCTT ATGTAGAAGA GAAAGAACAA TGGCAAGAAC AAATTAATCA GCTAAAGAAA 
CAACTTGATT TCAGTGTCAA TATTTGTCAG ACTTTAATGC AAGACCAGCA GACTCTGTCT 
TGTCTGCTAC AAACTCTTCT CACGAGTCCT TATAGTGTTA TGCCCAGCAG TGTTGCATCC 
CCGCAAGTGC ACCTTATAAT GCACCAGTTG AATCAGTGCT ATACCCAGCT AACGTGGCAG 
CAGAACAATG TCCAGAGGTT GAAACAAATG CTCAGTGAAC TCATGCGCCA ACAAAATCAG 
CATCCAGAAA AACCTGCAAG CAAGGAAGGA GGCAGTGGTA CATCACACCC TCCTTCTCCC 
AGTTTATTTT GTCCTTTCAA CTTTCCAACT CAGCCTGTAA ATCTGTTTAA CCTACCGGGG 
TTTACTAATT TTTCGTCATT TGCACCAAGT ATGAATTTCA GCCCTTTATT TCCTTCTAAT 
TTTGGAGATT TTTCTCAAAA TATTTCTACA CCCACTGAAC AGCAGCAACC GTTAGCCCAG 
AATCCTTCAG GGAAAACGGA ATACATGGCT TTTCCAAAAC CTTTTGAAAG TAGTTCTTCT 
ATTGGAGCGG AAAAACAAAG GAATCAAAAA CAGCCTGAAG AGGAGGTGGA GAACAGTAGG 
ACACCATGGT TTTATGACCA AGAAGGTGAA GTAGAAAAGC CATTTCTCAA GACTGGATTT 
GCAGTGTCTG TAGAGAAAAC AACAAATAGT AATCGCAAAA ATCAACTAGA TACCAGTAGG 
AGAAGACGCC AGTTTGATGA AGTGTCCTTA GAAAGCTTTA ACAGTATGCT TGACCCAGTA 
GATCCAACAA CAGTAACTAA AACATTCAAG TCAAGAAAAG CATCTGCACA GGCCAGCCTG 
GCATCTAAAG ATAAAACTCC CAAATCAAAG AGTAAGAAGA GGCATTCTAC TCAGCTGAAA 
AGCAGAGTGA AAAATATCGA AACCGGGAGT GATTTTTCCA TGTTCGAAGC TTTGCGGGAT 
ACCATTTATT CTGAAGTAGC TACGTTGATT TCTCAAAATG AATCTCGTCC ACATTTTCTT 
ATTGAACTCT TCCATGAGCT TCAGCTACTT AATACAGACT ATTTGAGACA GAGGGCCTTA 
TATGCATTGC AGGACATAGT ATCCAGGCAT ATTTCTGAGA ACCATGAAAA AGAAGGAGAA 
AATATAAAGT CCGTGAATTC TGGTACTTGG ATAGCATCCA ACTCAGAACT TACTCCCAGT 
GAAAGCCTTG CTACTACTGA TGATGAAACT TTTGAGAAGA ACTTTGAGAG AGAAACACAT 
AAAATAAGTG AGCAAAATGA TGCTGATAAT GCTAGTGTAT CTTCAACTTG TGAGCCTTTT 
GCAACAGATG TTCTGGGCGA CACTGTGATT CACTTAGATC AGGCATTAGC CAGAATGAGA 
GACTATACGC ATATGAAGAT GGAGGCTGAA AGTAGTCCAG ACGTCAGGTG CACCTGCAGG 
ATCATTGAGG ATGACGATGG TGCTGCTGCC ACAACCACGA TTAGTAATAA TTTAGAAGAA 
ACTCCTGTTA TTGAAAATCA TAGTTCACAG CAACCCGTGA GTGAAGTTGC TACCGTCCCG 
TGTCCTAGAA TTGATACTCA GCAACTGGAC CGGCAAATTA AAGCAATTAT GAAAGAAGTC 
ATTCCTTTTT TGAAGGAGCA CATGGATGAA GTATGTTCTT CTCAACTTCT AACCTCAGTA 
AGACGCATGG TTTTGGTCCT TACTCAGCAA AACAATGAGA GCAAAGAGTT TGTAAAGTTC 
TTTCATAAGC AGCTTGGAAG TATATTACAG GATTCACTCG CAAAATTTGC TGGCAGAAAA 
CTGAAGGATT GTGGAGAAGA TCTTCTTGTA GAGATATCTG AAGTGTTATT TAATGAATTG 
GCTTTCTTTA AGCTCATGCA AGATTTGGAT AATAATAGTG TAACTGTTAA ACAGAGATGC 
AAAAGGAAAA TAGAAGCTGC TGGAGTGATA CAGTCTTATG CAAAAGAGGC CAAAAGGATT 
CTTGAAGGAG ATCACAGTTC ACCTGCTGGA GAAATTGATG ATGAAGACAA AGACAAGGAT 
GAGACGGAAA CAGTTAAGCA GACTCAAACG TCTGAGGTTT ATGATGGTGA CGGCCCCAAA 
AATGTGAGAT CTGATGTTTC TGATCAAGAG GATGATGAAG AAAGTGAAGA ATGTCCAGTG 
TCTATTAATT TGTCCAAAGC TGAAACTCAG GCTTTGACTA ATTACGGAAG TGGAGAAGAT 
GAGAATGAAG ATGAAGAAAT AGAAGAATTT GAAGAGGGAC CTGTAGATGT CCAGACGTCA 
CTTCAGGCTA ACACTGAAAC TACAGAAGAA AATGAACACG ATGATCAGGT TCCACAGCAT 
GATTTTGAAA AATCATCAGA AAGCAAAAAT GTCCCATCAG AACAAGAACC CACTACTAGT 
AAAGGTGACC AGAATAGCAC TCCTGTGAAG CCCTGTTACC TCAGTATCGT GGAAAATGAG 
CAACCTTTAA ATAGCCCTGT CCAGAAGGAC ACTCTCACTA CCATCGACTC ATCCAGTCAG 
CCTAACCCTT TGCCTTTACC TTTAACTGAA ATTGAAACCT TGGTGCCTAG AGTCAAAGAA 
GTGAAATCTG CTCAGGAGAC TCCTGAAAGT TCTTTGGCTG GAAGTCCTGA TACTGAATCT 
CCAGTGTTAG TGAATGACTA TGAAGCAGAA TCTGGTAACA TAAGTCAAAA ATCTGATGAA 
GAAGACTTTG TGAAAGTTGA AGATTTACCA CTTAAACTGA CAATATATTC AGAGGCAGAT 
CTAAGGAAGA AAATGGCCGA AGAAGAACAG AAAAACCACT TATCTGGTGA AATATGTGCA 
ATGCAGACTG AAGAATTAGC TGGGAATTCT CAGACACTAA AAGAACCTGA AACAGTGGGA 
GCCCAAAGTA CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( XM_025279509.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025135294.1 Bubalus bubalis pericentriolar material 1 (PCM1), transcript variant X19, mRNA.

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CloneID	OBu42307
Clone ID Related Accession (Same CDS sequence)	XM_025279509.1
Accession Version	XM_025279509.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6291bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	pericentriolar material 1 protein isoform X18
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037545.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG GAGGAGGTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
AGCAATGAGA GTGTTGATGA CCGACTCAAC AATATGGATT GGGGCAGCCA GCAGAAGAAG 
GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA 
ACTAACGATA TTTCTCCGGA GTCATCACCG GGAGTTGGAA GACGAAGAAC AAAGACTTCA 
CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TCCCCGAGCA GGCAGAACTA 
GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCTCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCCAC TGTAAGTCCT 
CAAAAGAGAG AAATGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAGATTCGTG AGTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGACCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTCACT 
CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT CCAGAAGATT 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCGGT CTGAGGCTTC AGATGCCACG 
GCCAGAGATC CTCAGCAGGA GCCTATGGAA GAGATAGAAA ATTTGAAGAA ACAACATGAT 
TTATTAAAAA GGATGTTACA GCAGCAGGAG CAGCTGAGAG CTCTACAAGG ACGACAAGCT 
GCTCTGCTTG CCCTGCAGCA TAAAGCTGAG CAGGCTATAG CTGTGATGGA TGATTCTGAA 
AAGGTTGCGG AGACAACTCC TGGCCATCAT ACTGTACCAG GCAGACAGCC GGCTCATTCA 
CCTTTGCATC AGAGAGTACC CATAAGAGAA ACTACAGGTA GCCTATCTGG AGTCAGTATC 
ACCTCTGAAC TAAACGAAGA ATTGAATGAT TTAATTCAGC ATTTTCATAA TCAGCTTCGT 
GATTCTCAGC CTCCAGCTGT TCCAGACAAT AGAAGACAAG CAGAAAGTCT TTCATTAACT 
AGGGAGATTT CTCAGAGCAG AAATCCTTCA GTTTCAGAGC ATTTACCTGA TGAGAAAGTC 
CAACTTTTTA GCAAGATGAG AGTGTTACAA GAAAAGAAAC AAAAAATGGA CAAACTTCTT 
GGAGAACTTC ATACACTTCG AGATCAGCAT TTGAACAATT CATCATTTGT GCCTTCTTCA 
GCCTCTCCAC AAAGGAGTGT GGATCAGAGA AGTACAACCA CAGCTCCTTC TGCTCCTGTA 
GTCTTAACAC CAGTGGTCAA TGGAGAATCC AATAACCTCA CGCCATCTGT TCCTTATCCT 
GCTGCTTCTC TAGTTTCTCA GAATCAGAAT GAAAATGAAG GCCACCTAAA TCCAACTGAA 
AAACTCCAGA AGTTAAATGA AGTTCGAAAG AGGTTGAATG AACTAAGAGA ATTAGTTCAT 
TATTATGAGC AAACATCAGA TATGATGACA GATGCTGTGA ATGAAAACAC AAAAGAAGAA 
GAAACTGAAG AATCAGAATA TGATTCGGAG CATGAAAACT CGGAACCTGT TACTAACATT 
CGAAATCCAC AAGTGGCTGC CACCTGGAAC GAAGTAAATA GTAATTCTAA TGCACAGTGT 
GTTTCCAACA ATAGAGATGG GCGAGCAGTT AATTCTAACT GTGAAATTAA CAACAGGTCT 
GCTGCCAACA TAAGGGCTCT AAACATGCCT CCTCTTTCAG ATTGTCGATA CAATAGAGAA 
GGAGAACAGG AGATGCCTGT TGCACAAGGT CATGAGGAGG AGGAGGAGGA GGAGGAGGAA 
GCAGAAGATG AGGCCCTCAG TGGAGCTTCA CTGTCTAGTC ACAGCAGCAG CCTGATCATT 
GAGACTCCTG AAGATGCTGA ATTTGAACAT AAGATCCAGA AGCTTATGGC TGCAAAACTG 
AAACTTAGAC ACTTACAGAA CCTTGTAGCC ATGGTGCAGG ACGATGATGC AGCCAATCAA 
GGAGTTACTT CTGTGGAGGA TTTCTTCCCA GCAGAAGACA CCAAACAAAA TGCAAATAAC 
ACTAGAGGAA ATGCCAATAA AACACAGAAA GATGCTGGAG TAAATGAAAA GGCAAGGGAG 
AAATTTTATG AGGCTAAACT ACAGCAGCAA CAGAGAGAAC TCAAACAATT GCAGGAAGAA 
AGAAAGAAAC TGATTGAGAT TCAAGAGAAA ATTCAAGAAT TGCAAAAGGC ATGTCCTAAC 
CTACAGCTGT CAGCTGCTAG TGTGGGTAAT TGTCCCACAA AAACACATAT GCCAGCTGTT 
ACTTCATCCC CAACCGTTAA TGAAAATGAA GCCAGTACAA ACAAATCTGG TTTTGAGCCT 
CATGATTCTT CAGTGGTAGA TAATGAGGTA TGGTCAGATA TGCGAAGACA TGAAATGTTA 
AGGGAGGAGC TACGACAGAG AAGAAAGCAG CTGGAAGCTC TGATGGCTGA ACATCAGAGG 
AGGCAAGGTC TGGCTGAAAC TGCATCTCCA GTGGCTGTTT CACTGAGAAG TGATGGATCT 
GAGAACCTGT GTACACCTCA GCAAAGCAGA ACAGAAAAAA CAATGGCAAC TTGGGGAGGT 
TCTACCCAGT GTGCCCTAGA TGAAGAAGGA GATGAAGATG GTTACCTTTC TGAAGGAATT 
ATTCGGACAG ACGAAGAGGA GGAAGAGGAG GAGCAAGATG CCAGTTCCAA TGATAACTTT 
CATATGTATC CTCCAAACAG TGCAAATCAT AATTCTTACA ATGTAAAGGA AACTAAAAAT 
AGGTGGAAGA ACAATCGCCC TTTTTCAGCA GATGGAAATT ATCGTCCTTT AGCCAAGACA 
AGACAGCAGA ATATCAGCAT GCAGCGTCAG GAAAACCTTC GCTGGGTGTC CGAACTCTCT 
TATGTAGAAG AGAAAGAACA ATGGCAAGAA CAAATTAATC AGCTAAAGAA ACAACTTGAT 
TTCAGTGTCA ATATTTGTCA GACTTTAATG CAAGACCAGC AGACTCTGTC TTGTCTGCTA 
CAAACTCTTC TCACGAGTCC TTATAGTGTT ATGCCCAGCA GTGTTGCATC CCCGCAAGTG 
CACCTTATAA TGCACCAGTT GAATCAGTGC TATACCCAGC TAACGTGGCA GCAGAACAAT 
GTCCAGAGGT TGAAACAAAT GCTCAGTGAA CTCATGCGCC AACAAAATCA GCATCCAGAA 
AAACCTGCAA GCAAGGAAGG AGGCAGTGGT ACATCACACC CTCCTTCTCC CAGTTTATTT 
TGTCCTTTCA ACTTTCCAAC TCAGCCTGTA AATCTGTTTA ACCTACCGGG GTTTACTAAT 
TTTTCGTCAT TTGCACCAAG TATGAATTTC AGCCCTTTAT TTCCTTCTAA TTTTGGAGAT 
TTTTCTCAAA ATATTTCTAC ACCCACTGAA CAGCAGCAAC CGTTAGCCCA GAATCCTTCA 
GGGAAAACGG AATACATGGC TTTTCCAAAA CCTTTTGAAA GTAGTTCTTC TATTGGAGCG 
GAAAAACAAA GGAATCAAAA ACAGCCTGAA GAGGAGGTGG AGAACAGTAG GACACCATGG 
TTTTATGACC AAGAAGGTGA AGTAGAAAAG CCATTTCTCA AGACTGGATT TGCAGTGTCT 
GTAGAGAAAA CAACAAATAG TAATCGCAAA AATCAACTAG ATACCAGTAG GAGAAGACGC 
CAGTTTGATG AAGTGTCCTT AGAAAGCTTT AACAGTATGC TTGACCCAGT AGATCCAACA 
ACAGTAACTA AAACATTCAA GTCAAGAAAA GCATCTGCAC AGGCCAGCCT GGCATCTAAA 
GATAAAACTC CCAAATCAAA GAGTAAGAAG AGGCATTCTA CTCAGCTGAA AAGCAGAGTG 
AAAAATATCG GGTATGAAAG TGCCAGTGTG TCTAGCACAT GTGAACCTTG CAAAAGTAGA 
AACAGACATT CAGCCCAAAC TGAAGAGCCT GTTCAAGCAA AAGTACTCAG CAGAAAGAAT 
CATGAGCAGC TGGAAAAAGT CATAAGATGT AGTAGGTCTA CAGAAATATC TTCAGAAACC 
GGGAGTGATT TTTCCATGTT CGAAGCTTTG CGGGATACCA TTTATTCTGA AGTAGCTACG 
TTGATTTCTC AAAATGAATC TCGTCCACAT TTTCTTATTG AACTCTTCCA TGAGCTTCAG 
CTACTTAATA CAGACTATTT GAGACAGAGG GCCTTATATG CATTGCAGGA CATAGTATCC 
AGGCATATTT CTGAGAACCA TGAAAAAGAA GGAGAAAATA TAAAGTCCGT GAATTCTGGT 
ACTTGGATAG CATCCAACTC AGAACTTACT CCCAGTGAAA GCCTTGCTAC TACTGATGAT 
GAAACTTTTG AGAAGAACTT TGAGAGAGAA ACACATAAAA TAAGTGAGCA AAATGATGCT 
GATAATGCTA GTGTATCTTC AACTTGTGAG CCTTTTGCAA CAGATGTTCT GGGCGACACT 
GTGATTCACT TAGATCAGGC ATTAGCCAGA ATGAGAGACT ATACGCATAT GAAGATGGAG 
GCTGAAAGTA GTCCAGACGT CAGGTGCACC TGCAGGATCA TTGAGGATGA CGATGGTGCT 
GCTGCCACAA CCACGATTAG TAATAATTTA GAAGAAACTC CTGTTATTGA AAATCATAGT 
TCACAGCAAC CCGTGAGTGA AGTTGCTACC GTCCCGTGTC CTAGAATTGA TACTCAGCAA 
CTGGACCGGC AAATTAAAGC AATTATGAAA GAAGTCATTC CTTTTTTGAA GGAGCACATG 
GATGAAGTAT GTTCTTCTCA ACTTCTAACC TCAGTAAGAC GCATGGTTTT GGTCCTTACT 
CAGCAAAACA ATGAGAGCAA AGAGTTTGTA AAGTTCTTTC ATAAGCAGCT TGGAAGTATA 
TTACAGGATT CACTCGCAAA ATTTGCTGGC AGAAAACTGA AGGATTGTGG AGAAGATCTT 
CTTGTAGAGA TATCTGAAGT GTTATTTAAT GAATTGGCTT TCTTTAAGCT CATGCAAGAT 
TTGGATAATA ATAGTGTAAC TGTTAAACAG AGATGCAAAA GGAAAATAGA AGCTGCTGGA 
GTGATACAGT CTTATGCAAA AGAGGCCAAA AGGATTCTTG AAGGAGATCA CAGTTCACCT 
GCTGGAGAAA TTGATGATGA AGACAAAGAC AAGGATGAGA CGGAAACAGT TAAGCAGACT 
CAAACGTCTG AGGTTTATGA TGGTGACGGC CCCAAAAATG TGAGATCTGA TGTTTCTGAT 
CAAGAGGATG ATGAAGAAAG TGAAGAATGT CCAGTGTCTA TTAATTTGTC CAAAGCTGAA 
ACTCAGGCTT TGACTAATTA CGGAAGTGGA GAAGATGAGA ATGAAGATGA AGAAATAGAA 
GAATTTGAAG AGGGACCTGT AGATGTCCAG ACGTCACTTC AGGCTAACAC TGAAACTACA 
GAAGAAAATG AACACGATGA TCAGGTTCCA CAGCATGATT TTGAAAAATC ATCAGAAAGC 
AAAAATGTCC CATCAGAACA AGAACCCACT ACTAGTAAAG GTGACCAGAA TAGCACTCCT 
GTGAAGCCCT GTTACCTCAG TATCGTGGAA AATGAGCAAC CTTTAAATAG CCCTGTCCAG 
AAGGACACTC TCACTACCAT CGACTCATCC AGTCAGCCTA ACCCTTTGCC TTTACCTTTA 
ACTGAAATTG AAACCTTGGT GCCTAGAGTC AAAGAAGTGA AATCTGCTCA GGAGACTCCT 
GAAAGTTCTT TGGCTGGAAG TCCTGATACT GAATCTCCAG TGTTAGTGAA TGACTATGAA 
GCAGAATCTG GTAACATAAG TCAAAAATCT GATGAAGAAG ACTTTGTGAA AGTTGAAGAT 
TTACCACTTA AACTGACAAT ATATTCAGAG GCAGATCTAA GGAAGAAAAT GGCCGAAGAA 
GAACAGAAAA ACCACTTATC TGGTGAAATA TGTGCAATGC AGACTGAAGA ATTAGCTGGG 
AATTCTCAGA CACTAAAAGA ACCTGAAACA GTGGGAGCCC AAAGTACATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025135294.1
CDS	392..6682
Translation	MATGGGPFEEGVNDQDLPNWSNESVDDRLNNMDWGSQQKKANRSSEKNKRKFGVESDKRVTNDISPESSPGVGRRRTKTSLTFPHSRYMTQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDATVSPQKREMIGSAQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIREYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLQKILARENEEEDVRTIDSAVGSGSVAESTSLNIDVRSEASDATARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSEKVAETTPGHHTVPGRQPAHSPLHQRVPIRETTGSLSGVSITSELNEELNDLIQHFHNQLRDSQPPAVPDNRRQAESLSLTREISQSRNPSVSEHLPDEKVQLFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSFVPSSASPQRSVDQRSTTTAPSAPVVLTPVVNGESNNLTPSVPYPAASLVSQNQNENEGHLNPTEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENTKEEETEESEYDSEHENSEPVTNIRNPQVAATWNEVNSNSNAQCVSNNRDGRAVNSNCEINNRSAANIRALNMPPLSDCRYNREGEQEMPVAQGHEEEEEEEEEAEDEALSGASLSSHSSSLIIETPEDAEFEHKIQKLMAAKLKLRHLQNLVAMVQDDDAANQGVTSVEDFFPAEDTKQNANNTRGNANKTQKDAGVNEKAREKFYEAKLQQQQRELKQLQEERKKLIEIQEKIQELQKACPNLQLSAASVGNCPTKTHMPAVTSSPTVNENEASTNKSGFEPHDSSVVDNEVWSDMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETASPVAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGIIRTDEEEEEEEQDASSNDNFHMYPPNSANHNSYNVKETKNRWKNNRPFSADGNYRPLAKTRQQNISMQRQENLRWVSELSYVEEKEQWQEQINQLKKQLDFSVNICQTLMQDQQTLSCLLQTLLTSPYSVMPSSVASPQVHLIMHQLNQCYTQLTWQQNNVQRLKQMLSELMRQQNQHPEKPASKEGGSGTSHPPSPSLFCPFNFPTQPVNLFNLPGFTNFSSFAPSMNFSPLFPSNFGDFSQNISTPTEQQQPLAQNPSGKTEYMAFPKPFESSSSIGAEKQRNQKQPEEEVENSRTPWFYDQEGEVEKPFLKTGFAVSVEKTTNSNRKNQLDTSRRRRQFDEVSLESFNSMLDPVDPTTVTKTFKSRKASAQASLASKDKTPKSKSKKRHSTQLKSRVKNIGYESASVSSTCEPCKSRNRHSAQTEEPVQAKVLSRKNHEQLEKVIRCSRSTEISSETGSDFSMFEALRDTIYSEVATLISQNESRPHFLIELFHELQLLNTDYLRQRALYALQDIVSRHISENHEKEGENIKSVNSGTWIASNSELTPSESLATTDDETFEKNFERETHKISEQNDADNASVSSTCEPFATDVLGDTVIHLDQALARMRDYTHMKMEAESSPDVRCTCRIIEDDDGAAATTTISNNLEETPVIENHSSQQPVSEVATVPCPRIDTQQLDRQIKAIMKEVIPFLKEHMDEVCSSQLLTSVRRMVLVLTQQNNESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSVTVKQRCKRKIEAAGVIQSYAKEAKRILEGDHSSPAGEIDDEDKDKDETETVKQTQTSEVYDGDGPKNVRSDVSDQEDDEESEECPVSINLSKAETQALTNYGSGEDENEDEEIEEFEEGPVDVQTSLQANTETTEENEHDDQVPQHDFEKSSESKNVPSEQEPTTSKGDQNSTPVKPCYLSIVENEQPLNSPVQKDTLTTIDSSSQPNPLPLPLTEIETLVPRVKEVKSAQETPESSLAGSPDTESPVLVNDYEAESGNISQKSDEEDFVKVEDLPLKLTIYSEADLRKKMAEEEQKNHLSGEICAMQTEELAGNSQTLKEPETVGAQST

Target ORF information:

RefSeq Version	XM_025279509.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis pericentriolar material 1 (PCM1), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025279509.1

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
AGCAATGAGA GTGTTGATGA CCGACTCAAC AATATGGATT GGGGCAGCCA GCAGAAGAAG 
GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA 
ACTAACGATA TTTCTCCGGA GTCATCACCG GGAGTTGGAA GACGAAGAAC AAAGACTTCA 
CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TCCCCGAGCA GGCAGAACTA 
GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCTCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCCAC TGTAAGTCCT 
CAAAAGAGAG AAATGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAGATTCGTG AGTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGACCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTCACT 
CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT CCAGAAGATT 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCGGT CTGAGGCTTC AGATGCCACG 
GCCAGAGATC CTCAGCAGGA GCCTATGGAA GAGATAGAAA ATTTGAAGAA ACAACATGAT 
TTATTAAAAA GGATGTTACA GCAGCAGGAG CAGCTGAGAG CTCTACAAGG ACGACAAGCT 
GCTCTGCTTG CCCTGCAGCA TAAAGCTGAG CAGGCTATAG CTGTGATGGA TGATTCTGAA 
AAGGTTGCGG AGACAACTCC TGGCCATCAT ACTGTACCAG GCAGACAGCC GGCTCATTCA 
CCTTTGCATC AGAGAGTACC CATAAGAGAA ACTACAGGTA GCCTATCTGG AGTCAGTATC 
ACCTCTGAAC TAAACGAAGA ATTGAATGAT TTAATTCAGC ATTTTCATAA TCAGCTTCGT 
GATTCTCAGC CTCCAGCTGT TCCAGACAAT AGAAGACAAG CAGAAAGTCT TTCATTAACT 
AGGGAGATTT CTCAGAGCAG AAATCCTTCA GTTTCAGAGC ATTTACCTGA TGAGAAAGTC 
CAACTTTTTA GCAAGATGAG AGTGTTACAA GAAAAGAAAC AAAAAATGGA CAAACTTCTT 
GGAGAACTTC ATACACTTCG AGATCAGCAT TTGAACAATT CATCATTTGT GCCTTCTTCA 
GCCTCTCCAC AAAGGAGTGT GGATCAGAGA AGTACAACCA CAGCTCCTTC TGCTCCTGTA 
GTCTTAACAC CAGTGGTCAA TGGAGAATCC AATAACCTCA CGCCATCTGT TCCTTATCCT 
GCTGCTTCTC TAGTTTCTCA GAATCAGAAT GAAAATGAAG GCCACCTAAA TCCAACTGAA 
AAACTCCAGA AGTTAAATGA AGTTCGAAAG AGGTTGAATG AACTAAGAGA ATTAGTTCAT 
TATTATGAGC AAACATCAGA TATGATGACA GATGCTGTGA ATGAAAACAC AAAAGAAGAA 
GAAACTGAAG AATCAGAATA TGATTCGGAG CATGAAAACT CGGAACCTGT TACTAACATT 
CGAAATCCAC AAGTGGCTGC CACCTGGAAC GAAGTAAATA GTAATTCTAA TGCACAGTGT 
GTTTCCAACA ATAGAGATGG GCGAGCAGTT AATTCTAACT GTGAAATTAA CAACAGGTCT 
GCTGCCAACA TAAGGGCTCT AAACATGCCT CCTCTTTCAG ATTGTCGATA CAATAGAGAA 
GGAGAACAGG AGATGCCTGT TGCACAAGGT CATGAGGAGG AGGAGGAGGA GGAGGAGGAA 
GCAGAAGATG AGGCCCTCAG TGGAGCTTCA CTGTCTAGTC ACAGCAGCAG CCTGATCATT 
GAGACTCCTG AAGATGCTGA ATTTGAACAT AAGATCCAGA AGCTTATGGC TGCAAAACTG 
AAACTTAGAC ACTTACAGAA CCTTGTAGCC ATGGTGCAGG ACGATGATGC AGCCAATCAA 
GGAGTTACTT CTGTGGAGGA TTTCTTCCCA GCAGAAGACA CCAAACAAAA TGCAAATAAC 
ACTAGAGGAA ATGCCAATAA AACACAGAAA GATGCTGGAG TAAATGAAAA GGCAAGGGAG 
AAATTTTATG AGGCTAAACT ACAGCAGCAA CAGAGAGAAC TCAAACAATT GCAGGAAGAA 
AGAAAGAAAC TGATTGAGAT TCAAGAGAAA ATTCAAGAAT TGCAAAAGGC ATGTCCTAAC 
CTACAGCTGT CAGCTGCTAG TGTGGGTAAT TGTCCCACAA AAACACATAT GCCAGCTGTT 
ACTTCATCCC CAACCGTTAA TGAAAATGAA GCCAGTACAA ACAAATCTGG TTTTGAGCCT 
CATGATTCTT CAGTGGTAGA TAATGAGGTA TGGTCAGATA TGCGAAGACA TGAAATGTTA 
AGGGAGGAGC TACGACAGAG AAGAAAGCAG CTGGAAGCTC TGATGGCTGA ACATCAGAGG 
AGGCAAGGTC TGGCTGAAAC TGCATCTCCA GTGGCTGTTT CACTGAGAAG TGATGGATCT 
GAGAACCTGT GTACACCTCA GCAAAGCAGA ACAGAAAAAA CAATGGCAAC TTGGGGAGGT 
TCTACCCAGT GTGCCCTAGA TGAAGAAGGA GATGAAGATG GTTACCTTTC TGAAGGAATT 
ATTCGGACAG ACGAAGAGGA GGAAGAGGAG GAGCAAGATG CCAGTTCCAA TGATAACTTT 
CATATGTATC CTCCAAACAG TGCAAATCAT AATTCTTACA ATGTAAAGGA AACTAAAAAT 
AGGTGGAAGA ACAATCGCCC TTTTTCAGCA GATGGAAATT ATCGTCCTTT AGCCAAGACA 
AGACAGCAGA ATATCAGCAT GCAGCGTCAG GAAAACCTTC GCTGGGTGTC CGAACTCTCT 
TATGTAGAAG AGAAAGAACA ATGGCAAGAA CAAATTAATC AGCTAAAGAA ACAACTTGAT 
TTCAGTGTCA ATATTTGTCA GACTTTAATG CAAGACCAGC AGACTCTGTC TTGTCTGCTA 
CAAACTCTTC TCACGAGTCC TTATAGTGTT ATGCCCAGCA GTGTTGCATC CCCGCAAGTG 
CACCTTATAA TGCACCAGTT GAATCAGTGC TATACCCAGC TAACGTGGCA GCAGAACAAT 
GTCCAGAGGT TGAAACAAAT GCTCAGTGAA CTCATGCGCC AACAAAATCA GCATCCAGAA 
AAACCTGCAA GCAAGGAAGG AGGCAGTGGT ACATCACACC CTCCTTCTCC CAGTTTATTT 
TGTCCTTTCA ACTTTCCAAC TCAGCCTGTA AATCTGTTTA ACCTACCGGG GTTTACTAAT 
TTTTCGTCAT TTGCACCAAG TATGAATTTC AGCCCTTTAT TTCCTTCTAA TTTTGGAGAT 
TTTTCTCAAA ATATTTCTAC ACCCACTGAA CAGCAGCAAC CGTTAGCCCA GAATCCTTCA 
GGGAAAACGG AATACATGGC TTTTCCAAAA CCTTTTGAAA GTAGTTCTTC TATTGGAGCG 
GAAAAACAAA GGAATCAAAA ACAGCCTGAA GAGGAGGTGG AGAACAGTAG GACACCATGG 
TTTTATGACC AAGAAGGTGA AGTAGAAAAG CCATTTCTCA AGACTGGATT TGCAGTGTCT 
GTAGAGAAAA CAACAAATAG TAATCGCAAA AATCAACTAG ATACCAGTAG GAGAAGACGC 
CAGTTTGATG AAGTGTCCTT AGAAAGCTTT AACAGTATGC TTGACCCAGT AGATCCAACA 
ACAGTAACTA AAACATTCAA GTCAAGAAAA GCATCTGCAC AGGCCAGCCT GGCATCTAAA 
GATAAAACTC CCAAATCAAA GAGTAAGAAG AGGCATTCTA CTCAGCTGAA AAGCAGAGTG 
AAAAATATCG GGTATGAAAG TGCCAGTGTG TCTAGCACAT GTGAACCTTG CAAAAGTAGA 
AACAGACATT CAGCCCAAAC TGAAGAGCCT GTTCAAGCAA AAGTACTCAG CAGAAAGAAT 
CATGAGCAGC TGGAAAAAGT CATAAGATGT AGTAGGTCTA CAGAAATATC TTCAGAAACC 
GGGAGTGATT TTTCCATGTT CGAAGCTTTG CGGGATACCA TTTATTCTGA AGTAGCTACG 
TTGATTTCTC AAAATGAATC TCGTCCACAT TTTCTTATTG AACTCTTCCA TGAGCTTCAG 
CTACTTAATA CAGACTATTT GAGACAGAGG GCCTTATATG CATTGCAGGA CATAGTATCC 
AGGCATATTT CTGAGAACCA TGAAAAAGAA GGAGAAAATA TAAAGTCCGT GAATTCTGGT 
ACTTGGATAG CATCCAACTC AGAACTTACT CCCAGTGAAA GCCTTGCTAC TACTGATGAT 
GAAACTTTTG AGAAGAACTT TGAGAGAGAA ACACATAAAA TAAGTGAGCA AAATGATGCT 
GATAATGCTA GTGTATCTTC AACTTGTGAG CCTTTTGCAA CAGATGTTCT GGGCGACACT 
GTGATTCACT TAGATCAGGC ATTAGCCAGA ATGAGAGACT ATACGCATAT GAAGATGGAG 
GCTGAAAGTA GTCCAGACGT CAGGTGCACC TGCAGGATCA TTGAGGATGA CGATGGTGCT 
GCTGCCACAA CCACGATTAG TAATAATTTA GAAGAAACTC CTGTTATTGA AAATCATAGT 
TCACAGCAAC CCGTGAGTGA AGTTGCTACC GTCCCGTGTC CTAGAATTGA TACTCAGCAA 
CTGGACCGGC AAATTAAAGC AATTATGAAA GAAGTCATTC CTTTTTTGAA GGAGCACATG 
GATGAAGTAT GTTCTTCTCA ACTTCTAACC TCAGTAAGAC GCATGGTTTT GGTCCTTACT 
CAGCAAAACA ATGAGAGCAA AGAGTTTGTA AAGTTCTTTC ATAAGCAGCT TGGAAGTATA 
TTACAGGATT CACTCGCAAA ATTTGCTGGC AGAAAACTGA AGGATTGTGG AGAAGATCTT 
CTTGTAGAGA TATCTGAAGT GTTATTTAAT GAATTGGCTT TCTTTAAGCT CATGCAAGAT 
TTGGATAATA ATAGTGTAAC TGTTAAACAG AGATGCAAAA GGAAAATAGA AGCTGCTGGA 
GTGATACAGT CTTATGCAAA AGAGGCCAAA AGGATTCTTG AAGGAGATCA CAGTTCACCT 
GCTGGAGAAA TTGATGATGA AGACAAAGAC AAGGATGAGA CGGAAACAGT TAAGCAGACT 
CAAACGTCTG AGGTTTATGA TGGTGACGGC CCCAAAAATG TGAGATCTGA TGTTTCTGAT 
CAAGAGGATG ATGAAGAAAG TGAAGAATGT CCAGTGTCTA TTAATTTGTC CAAAGCTGAA 
ACTCAGGCTT TGACTAATTA CGGAAGTGGA GAAGATGAGA ATGAAGATGA AGAAATAGAA 
GAATTTGAAG AGGGACCTGT AGATGTCCAG ACGTCACTTC AGGCTAACAC TGAAACTACA 
GAAGAAAATG AACACGATGA TCAGGTTCCA CAGCATGATT TTGAAAAATC ATCAGAAAGC 
AAAAATGTCC CATCAGAACA AGAACCCACT ACTAGTAAAG GTGACCAGAA TAGCACTCCT 
GTGAAGCCCT GTTACCTCAG TATCGTGGAA AATGAGCAAC CTTTAAATAG CCCTGTCCAG 
AAGGACACTC TCACTACCAT CGACTCATCC AGTCAGCCTA ACCCTTTGCC TTTACCTTTA 
ACTGAAATTG AAACCTTGGT GCCTAGAGTC AAAGAAGTGA AATCTGCTCA GGAGACTCCT 
GAAAGTTCTT TGGCTGGAAG TCCTGATACT GAATCTCCAG TGTTAGTGAA TGACTATGAA 
GCAGAATCTG GTAACATAAG TCAAAAATCT GATGAAGAAG ACTTTGTGAA AGTTGAAGAT 
TTACCACTTA AACTGACAAT ATATTCAGAG GCAGATCTAA GGAAGAAAAT GGCCGAAGAA 
GAACAGAAAA ACCACTTATC TGGTGAAATA TGTGCAATGC AGACTGAAGA ATTAGCTGGG 
AATTCTCAGA CACTAAAAGA ACCTGAAACA GTGGGAGCCC AAAGTACATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( XM_025279513.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025135298.1 Bubalus bubalis pericentriolar material 1 (PCM1), transcript variant X20, mRNA.

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CloneID	OBu42308
Clone ID Related Accession (Same CDS sequence)	XM_025279513.1
Accession Version	XM_025279513.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6174bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	pericentriolar material 1 protein isoform X19
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037545.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG GAGGAGGTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
AGCAATGAGA GTGTTGATGA CCGACTCAAC AATATGGATT GGGGCAGCCA GCAGAAGAAG 
GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA 
ACTAACGATA TTTCTCCGGA GTCATCACCG GGAGTTGGAA GACGAAGAAC AAAGACTTCA 
CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TCCCCGAGCA GGCAGAACTA 
GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCTCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCCAC TGTAAGTCCT 
CAAAAGAGAG AAATGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAGATTCGTG AGTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGACCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTCACT 
CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT CCAGAAGATT 
CTTGCCAGAG ATCCTCAGCA GGAGCCTATG GAAGAGATAG AAAATTTGAA GAAACAACAT 
GATTTATTAA AAAGGATGTT ACAGCAGCAG GAGCAGCTGA GAGCTCTACA AGGACGACAA 
GCTGCTCTGC TTGCCCTGCA GCATAAAGCT GAGCAGGCTA TAGCTGTGAT GGATGATTCT 
GAAAAGGTTG CGGAGACAAC TCCTGGCCAT CATACTGTAC CAGGCAGACA GCCGGCTCAT 
TCACCTTTGC ATCAGAGAGT ACCCATAAGA GAAACTACAG GTAGCCTATC TGGAGTCAGT 
ATCACCTCTG AACTAAACGA AGAATTGAAT GATTTAATTC AGCATTTTCA TAATCAGCTT 
CGTGATTCTC AGCCTCCAGC TGTTCCAGAC AATAGAAGAC AAGCAGAAAG TCTTTCATTA 
ACTAGGGAGA TTTCTCAGAG CAGAAATCCT TCAGTTTCAG AGCATTTACC TGATGAGAAA 
GTCCAACTTT TTAGCAAGAT GAGAGTGTTA CAAGAAAAGA AACAAAAAAT GGACAAACTT 
CTTGGAGAAC TTCATACACT TCGAGATCAG CATTTGAACA ATTCATCATT TGTGCCTTCT 
TCAGCCTCTC CACAAAGGAG TGTGGATCAG AGAAGTACAA CCACAGCTCC TTCTGCTCCT 
GTAGTCTTAA CACCAGTGGT CAATGGAGAA TCCAATAACC TCACGCCATC TGTTCCTTAT 
CCTGCTGCTT CTCTAGTTTC TCAGAATCAG AATGAAAATG AAGGCCACCT AAATCCAACT 
GAAAAACTCC AGAAGTTAAA TGAAGTTCGA AAGAGGTTGA ATGAACTAAG AGAATTAGTT 
CATTATTATG AGCAAACATC AGATATGATG ACAGATGCTG TGAATGAAAA CACAAAAGAA 
GAAGAAACTG AAGAATCAGA ATATGATTCG GAGCATGAAA ACTCGGAACC TGTTACTAAC 
ATTCGAAATC CACAAGTGGC TGCCACCTGG AACGAAGTAA ATAGTAATTC TAATGCACAG 
TGTGTTTCCA ACAATAGAGA TGGGCGAGCA GTTAATTCTA ACTGTGAAAT TAACAACAGG 
TCTGCTGCCA ACATAAGGGC TCTAAACATG CCTCCTCTTT CAGATTGTCG ATACAATAGA 
GAAGGAGAAC AGGAGATGCC TGTTGCACAA GGTCATGAGG AGGAGGAGGA GGAGGAGGAG 
GAAGCAGAAG ATGAGGCCCT CAGTGGAGCT TCACTGTCTA GTCACAGCAG CAGCCTGATC 
ATTGAGACTC CTGAAGATGC TGAATTTGAA CATAAGATCC AGAAGCTTAT GGCTGCAAAA 
CTGAAACTTA GACACTTACA GAACCTTGTA GCCATGGTGC AGGACGATGA TGCAGCCAAT 
CAAGGAGTTA CTTCTGTGGA GGATTTCTTC CCAGCAGAAG ACACCAAACA AAATGCAAAT 
AACACTAGAG GAAATGCCAA TAAAACACAG AAAGATGCTG GAGTAAATGA AAAGGCAAGG 
GAGAAATTTT ATGAGGCTAA ACTACAGCAG CAACAGAGAG AACTCAAACA ATTGCAGGAA 
GAAAGAAAGA AACTGATTGA GATTCAAGAG AAAATTCAAG AATTGCAAAA GGCATGTCCT 
AACCTACAGC TGTCAGCTGC TAGTGTGGGT AATTGTCCCA CAAAAACACA TATGCCAGCT 
GTTACTTCAT CCCCAACCGT TAATGAAAAT GAAGCCAGTA CAAACAAATC TGGTTTTGAG 
CCTCATGATT CTTCAGTGGT AGATAATGAG GTATGGTCAG ATATGCGAAG ACATGAAATG 
TTAAGGGAGG AGCTACGACA GAGAAGAAAG CAGCTGGAAG CTCTGATGGC TGAACATCAG 
AGGAGGCAAG GTCTGGCTGA AACTGCATCT CCAGTGGCTG TTTCACTGAG AAGTGATGGA 
TCTGAGAACC TGTGTACACC TCAGCAAAGC AGAACAGAAA AAACAATGGC AACTTGGGGA 
GGTTCTACCC AGTGTGCCCT AGATGAAGAA GGAGATGAAG ATGGTTACCT TTCTGAAGGA 
ATTATTCGGA CAGACGAAGA GGAGGAAGAG GAGGAGCAAG ATGCCAGTTC CAATGATAAC 
TTTCATATGT ATCCTCCAAA CAGTGCAAAT CATAATTCTT ACAATGTAAA GGAAACTAAA 
AATAGGTGGA AGAACAATCG CCCTTTTTCA GCAGATGGAA ATTATCGTCC TTTAGCCAAG 
ACAAGACAGC AGAATATCAG CATGCAGCGT CAGGAAAACC TTCGCTGGGT GTCCGAACTC 
TCTTATGTAG AAGAGAAAGA ACAATGGCAA GAACAAATTA ATCAGCTAAA GAAACAACTT 
GATTTCAGTG TCAATATTTG TCAGACTTTA ATGCAAGACC AGCAGACTCT GTCTTGTCTG 
CTACAAACTC TTCTCACGAG TCCTTATAGT GTTATGCCCA GCAGTGTTGC ATCCCCGCAA 
GTGCACCTTA TAATGCACCA GTTGAATCAG TGCTATACCC AGCTAACGTG GCAGCAGAAC 
AATGTCCAGA GGTTGAAACA AATGCTCAGT GAACTCATGC GCCAACAAAA TCAGCATCCA 
GAAAAACCTG CAAGCAAGGA AGGAGGCAGT GGTACATCAC ACCCTCCTTC TCCCAGTTTA 
TTTTGTCCTT TCAACTTTCC AACTCAGCCT GTAAATCTGT TTAACCTACC GGGGTTTACT 
AATTTTTCGT CATTTGCACC AAGTATGAAT TTCAGCCCTT TATTTCCTTC TAATTTTGGA 
GATTTTTCTC AAAATATTTC TACACCCACT GAACAGCAGC AACCGTTAGC CCAGAATCCT 
TCAGGGAAAA CGGAATACAT GGCTTTTCCA AAACCTTTTG AAAGTAGTTC TTCTATTGGA 
GCGGAAAAAC AAAGGAATCA AAAACAGCCT GAAGAGGAGG TGGAGAACAG TAGGACACCA 
TGGTTTTATG ACCAAGAAGG TGAAGTAGAA AAGCCATTTC TCAAGACTGG ATTTGCAGTG 
TCTGTAGAGA AAACAACAAA TAGTAATCGC AAAAATCAAC TAGATACCAG TAGGAGAAGA 
CGCCAGTTTG ATGAAGTGTC CTTAGAAAGC TTTAACAGTA TGCTTGACCC AGTAGATCCA 
ACAACAGTAA CTAAAACATT CAAGTCAAGA AAAGCATCTG CACAGGCCAG CCTGGCATCT 
AAAGATAAAA CTCCCAAATC AAAGAGTAAG AAGAGGCATT CTACTCAGCT GAAAAGCAGA 
GTGAAAAATA TCGGGTATGA AAGTGCCAGT GTGTCTAGCA CATGTGAACC TTGCAAAAGT 
AGAAACAGAC ATTCAGCCCA AACTGAAGAG CCTGTTCAAG CAAAAGTACT CAGCAGAAAG 
AATCATGAGC AGCTGGAAAA AGTCATAAGA TGTAGTAGGT CTACAGAAAT ATCTTCAGAA 
ACCGGGAGTG ATTTTTCCAT GTTCGAAGCT TTGCGGGATA CCATTTATTC TGAAGTAGCT 
ACGTTGATTT CTCAAAATGA ATCTCGTCCA CATTTTCTTA TTGAACTCTT CCATGAGCTT 
CAGCTACTTA ATACAGACTA TTTGAGACAG AGGGCCTTAT ATGCATTGCA GGACATAGTA 
TCCAGGCATA TTTCTGAGAA CCATGAAAAA GAAGGAGAAA ATATAAAGTC CGTGAATTCT 
GGTACTTGGA TAGCATCCAA CTCAGAACTT ACTCCCAGTG AAAGCCTTGC TACTACTGAT 
GATGAAACTT TTGAGAAGAA CTTTGAGAGA GAAACACATA AAATAAGTGA GCAAAATGAT 
GCTGATAATG CTAGTGTATC TTCAACTTGT GAGCCTTTTG CAACAGATGT TCTGGGCGAC 
ACTGTGATTC ACTTAGATCA GGCATTAGCC AGAATGAGAG ACTATACGCA TATGAAGATG 
GAGGCTGAAA GTAGTCCAGA CGTCAGGTGC ACCTGCAGGA TCATTGAGGA TGACGATGGT 
GCTGCTGCCA CAACCACGAT TAGTAATAAT TTAGAAGAAA CTCCTGTTAT TGAAAATCAT 
AGTTCACAGC AACCCGTGAG TGAAGTTGCT ACCGTCCCGT GTCCTAGAAT TGATACTCAG 
CAACTGGACC GGCAAATTAA AGCAATTATG AAAGAAGTCA TTCCTTTTTT GAAGGAGCAC 
ATGGATGAAG TATGTTCTTC TCAACTTCTA ACCTCAGTAA GACGCATGGT TTTGGTCCTT 
ACTCAGCAAA ACAATGAGAG CAAAGAGTTT GTAAAGTTCT TTCATAAGCA GCTTGGAAGT 
ATATTACAGG ATTCACTCGC AAAATTTGCT GGCAGAAAAC TGAAGGATTG TGGAGAAGAT 
CTTCTTGTAG AGATATCTGA AGTGTTATTT AATGAATTGG CTTTCTTTAA GCTCATGCAA 
GATTTGGATA ATAATAGTGT AACTGTTAAA CAGAGATGCA AAAGGAAAAT AGAAGCTGCT 
GGAGTGATAC AGTCTTATGC AAAAGAGGCC AAAAGGATTC TTGAAGGAGA TCACAGTTCA 
CCTGCTGGAG AAATTGATGA TGAAGACAAA GACAAGGATG AGACGGAAAC AGTTAAGCAG 
ACTCAAACGT CTGAGGTTTA TGATGGTGAC GGCCCCAAAA ATGTGAGATC TGATGTTTCT 
GATCAAGAGG ATGATGAAGA AAGTGAAGAA TGTCCAGTGT CTATTAATTT GTCCAAAGCT 
GAAACTCAGG CTTTGACTAA TTACGGAAGT GGAGAAGATG AGAATGAAGA TGAAGAAATA 
GAAGAATTTG AAGAGGGACC TGTAGATGTC CAGACGTCAC TTCAGGCTAA CACTGAAACT 
ACAGAAGAAA ATGAACACGA TGATCAGGTT CCACAGCATG ATTTTGAAAA ATCATCAGAA 
AGCAAAAATG TCCCATCAGA ACAAGAACCC ACTACTAGTA AAGGTGACCA GAATAGCACT 
CCTGTGAAGC CCTGTTACCT CAGTATCGTG GAAAATGAGC AACCTTTAAA TAGCCCTGTC 
CAGAAGGACA CTCTCACTAC CATCGACTCA TCCAGTCAGC CTAACCCTTT GCCTTTACCT 
TTAACTGAAA TTGAAACCTT GGTGCCTAGA GTCAAAGAAG TGAAATCTGC TCAGGAGACT 
CCTGAAAGTT CTTTGGCTGG AAGTCCTGAT ACTGAATCTC CAGTGTTAGT GAATGACTAT 
GAAGCAGAAT CTGGTAACAT AAGTCAAAAA TCTGATGAAG AAGACTTTGT GAAAGTTGAA 
GATTTACCAC TTAAACTGAC AATATATTCA GAGGCAGATC TAAGGAAGAA AATGGCCGAA 
GAAGAACAGA AAAACCACTT ATCTGGTGAA ATATGTGCAA TGCAGACTGA AGAATTAGCT 
GGGAATTCTC AGACACTAAA AGAACCTGAA ACAGTGGGAG CCCAAAGTAC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025135298.1
CDS	392..6565
Translation	MATGGGPFEEGVNDQDLPNWSNESVDDRLNNMDWGSQQKKANRSSEKNKRKFGVESDKRVTNDISPESSPGVGRRRTKTSLTFPHSRYMTQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDATVSPQKREMIGSAQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIREYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLQKILARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSEKVAETTPGHHTVPGRQPAHSPLHQRVPIRETTGSLSGVSITSELNEELNDLIQHFHNQLRDSQPPAVPDNRRQAESLSLTREISQSRNPSVSEHLPDEKVQLFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSFVPSSASPQRSVDQRSTTTAPSAPVVLTPVVNGESNNLTPSVPYPAASLVSQNQNENEGHLNPTEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENTKEEETEESEYDSEHENSEPVTNIRNPQVAATWNEVNSNSNAQCVSNNRDGRAVNSNCEINNRSAANIRALNMPPLSDCRYNREGEQEMPVAQGHEEEEEEEEEAEDEALSGASLSSHSSSLIIETPEDAEFEHKIQKLMAAKLKLRHLQNLVAMVQDDDAANQGVTSVEDFFPAEDTKQNANNTRGNANKTQKDAGVNEKAREKFYEAKLQQQQRELKQLQEERKKLIEIQEKIQELQKACPNLQLSAASVGNCPTKTHMPAVTSSPTVNENEASTNKSGFEPHDSSVVDNEVWSDMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETASPVAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGIIRTDEEEEEEEQDASSNDNFHMYPPNSANHNSYNVKETKNRWKNNRPFSADGNYRPLAKTRQQNISMQRQENLRWVSELSYVEEKEQWQEQINQLKKQLDFSVNICQTLMQDQQTLSCLLQTLLTSPYSVMPSSVASPQVHLIMHQLNQCYTQLTWQQNNVQRLKQMLSELMRQQNQHPEKPASKEGGSGTSHPPSPSLFCPFNFPTQPVNLFNLPGFTNFSSFAPSMNFSPLFPSNFGDFSQNISTPTEQQQPLAQNPSGKTEYMAFPKPFESSSSIGAEKQRNQKQPEEEVENSRTPWFYDQEGEVEKPFLKTGFAVSVEKTTNSNRKNQLDTSRRRRQFDEVSLESFNSMLDPVDPTTVTKTFKSRKASAQASLASKDKTPKSKSKKRHSTQLKSRVKNIGYESASVSSTCEPCKSRNRHSAQTEEPVQAKVLSRKNHEQLEKVIRCSRSTEISSETGSDFSMFEALRDTIYSEVATLISQNESRPHFLIELFHELQLLNTDYLRQRALYALQDIVSRHISENHEKEGENIKSVNSGTWIASNSELTPSESLATTDDETFEKNFERETHKISEQNDADNASVSSTCEPFATDVLGDTVIHLDQALARMRDYTHMKMEAESSPDVRCTCRIIEDDDGAAATTTISNNLEETPVIENHSSQQPVSEVATVPCPRIDTQQLDRQIKAIMKEVIPFLKEHMDEVCSSQLLTSVRRMVLVLTQQNNESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSVTVKQRCKRKIEAAGVIQSYAKEAKRILEGDHSSPAGEIDDEDKDKDETETVKQTQTSEVYDGDGPKNVRSDVSDQEDDEESEECPVSINLSKAETQALTNYGSGEDENEDEEIEEFEEGPVDVQTSLQANTETTEENEHDDQVPQHDFEKSSESKNVPSEQEPTTSKGDQNSTPVKPCYLSIVENEQPLNSPVQKDTLTTIDSSSQPNPLPLPLTEIETLVPRVKEVKSAQETPESSLAGSPDTESPVLVNDYEAESGNISQKSDEEDFVKVEDLPLKLTIYSEADLRKKMAEEEQKNHLSGEICAMQTEELAGNSQTLKEPETVGAQST

Target ORF information:

RefSeq Version	XM_025279513.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis pericentriolar material 1 (PCM1), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025279513.1

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
AGCAATGAGA GTGTTGATGA CCGACTCAAC AATATGGATT GGGGCAGCCA GCAGAAGAAG 
GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA 
ACTAACGATA TTTCTCCGGA GTCATCACCG GGAGTTGGAA GACGAAGAAC AAAGACTTCA 
CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TCCCCGAGCA GGCAGAACTA 
GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCTCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCCAC TGTAAGTCCT 
CAAAAGAGAG AAATGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAGATTCGTG AGTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGACCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTCACT 
CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT CCAGAAGATT 
CTTGCCAGAG ATCCTCAGCA GGAGCCTATG GAAGAGATAG AAAATTTGAA GAAACAACAT 
GATTTATTAA AAAGGATGTT ACAGCAGCAG GAGCAGCTGA GAGCTCTACA AGGACGACAA 
GCTGCTCTGC TTGCCCTGCA GCATAAAGCT GAGCAGGCTA TAGCTGTGAT GGATGATTCT 
GAAAAGGTTG CGGAGACAAC TCCTGGCCAT CATACTGTAC CAGGCAGACA GCCGGCTCAT 
TCACCTTTGC ATCAGAGAGT ACCCATAAGA GAAACTACAG GTAGCCTATC TGGAGTCAGT 
ATCACCTCTG AACTAAACGA AGAATTGAAT GATTTAATTC AGCATTTTCA TAATCAGCTT 
CGTGATTCTC AGCCTCCAGC TGTTCCAGAC AATAGAAGAC AAGCAGAAAG TCTTTCATTA 
ACTAGGGAGA TTTCTCAGAG CAGAAATCCT TCAGTTTCAG AGCATTTACC TGATGAGAAA 
GTCCAACTTT TTAGCAAGAT GAGAGTGTTA CAAGAAAAGA AACAAAAAAT GGACAAACTT 
CTTGGAGAAC TTCATACACT TCGAGATCAG CATTTGAACA ATTCATCATT TGTGCCTTCT 
TCAGCCTCTC CACAAAGGAG TGTGGATCAG AGAAGTACAA CCACAGCTCC TTCTGCTCCT 
GTAGTCTTAA CACCAGTGGT CAATGGAGAA TCCAATAACC TCACGCCATC TGTTCCTTAT 
CCTGCTGCTT CTCTAGTTTC TCAGAATCAG AATGAAAATG AAGGCCACCT AAATCCAACT 
GAAAAACTCC AGAAGTTAAA TGAAGTTCGA AAGAGGTTGA ATGAACTAAG AGAATTAGTT 
CATTATTATG AGCAAACATC AGATATGATG ACAGATGCTG TGAATGAAAA CACAAAAGAA 
GAAGAAACTG AAGAATCAGA ATATGATTCG GAGCATGAAA ACTCGGAACC TGTTACTAAC 
ATTCGAAATC CACAAGTGGC TGCCACCTGG AACGAAGTAA ATAGTAATTC TAATGCACAG 
TGTGTTTCCA ACAATAGAGA TGGGCGAGCA GTTAATTCTA ACTGTGAAAT TAACAACAGG 
TCTGCTGCCA ACATAAGGGC TCTAAACATG CCTCCTCTTT CAGATTGTCG ATACAATAGA 
GAAGGAGAAC AGGAGATGCC TGTTGCACAA GGTCATGAGG AGGAGGAGGA GGAGGAGGAG 
GAAGCAGAAG ATGAGGCCCT CAGTGGAGCT TCACTGTCTA GTCACAGCAG CAGCCTGATC 
ATTGAGACTC CTGAAGATGC TGAATTTGAA CATAAGATCC AGAAGCTTAT GGCTGCAAAA 
CTGAAACTTA GACACTTACA GAACCTTGTA GCCATGGTGC AGGACGATGA TGCAGCCAAT 
CAAGGAGTTA CTTCTGTGGA GGATTTCTTC CCAGCAGAAG ACACCAAACA AAATGCAAAT 
AACACTAGAG GAAATGCCAA TAAAACACAG AAAGATGCTG GAGTAAATGA AAAGGCAAGG 
GAGAAATTTT ATGAGGCTAA ACTACAGCAG CAACAGAGAG AACTCAAACA ATTGCAGGAA 
GAAAGAAAGA AACTGATTGA GATTCAAGAG AAAATTCAAG AATTGCAAAA GGCATGTCCT 
AACCTACAGC TGTCAGCTGC TAGTGTGGGT AATTGTCCCA CAAAAACACA TATGCCAGCT 
GTTACTTCAT CCCCAACCGT TAATGAAAAT GAAGCCAGTA CAAACAAATC TGGTTTTGAG 
CCTCATGATT CTTCAGTGGT AGATAATGAG GTATGGTCAG ATATGCGAAG ACATGAAATG 
TTAAGGGAGG AGCTACGACA GAGAAGAAAG CAGCTGGAAG CTCTGATGGC TGAACATCAG 
AGGAGGCAAG GTCTGGCTGA AACTGCATCT CCAGTGGCTG TTTCACTGAG AAGTGATGGA 
TCTGAGAACC TGTGTACACC TCAGCAAAGC AGAACAGAAA AAACAATGGC AACTTGGGGA 
GGTTCTACCC AGTGTGCCCT AGATGAAGAA GGAGATGAAG ATGGTTACCT TTCTGAAGGA 
ATTATTCGGA CAGACGAAGA GGAGGAAGAG GAGGAGCAAG ATGCCAGTTC CAATGATAAC 
TTTCATATGT ATCCTCCAAA CAGTGCAAAT CATAATTCTT ACAATGTAAA GGAAACTAAA 
AATAGGTGGA AGAACAATCG CCCTTTTTCA GCAGATGGAA ATTATCGTCC TTTAGCCAAG 
ACAAGACAGC AGAATATCAG CATGCAGCGT CAGGAAAACC TTCGCTGGGT GTCCGAACTC 
TCTTATGTAG AAGAGAAAGA ACAATGGCAA GAACAAATTA ATCAGCTAAA GAAACAACTT 
GATTTCAGTG TCAATATTTG TCAGACTTTA ATGCAAGACC AGCAGACTCT GTCTTGTCTG 
CTACAAACTC TTCTCACGAG TCCTTATAGT GTTATGCCCA GCAGTGTTGC ATCCCCGCAA 
GTGCACCTTA TAATGCACCA GTTGAATCAG TGCTATACCC AGCTAACGTG GCAGCAGAAC 
AATGTCCAGA GGTTGAAACA AATGCTCAGT GAACTCATGC GCCAACAAAA TCAGCATCCA 
GAAAAACCTG CAAGCAAGGA AGGAGGCAGT GGTACATCAC ACCCTCCTTC TCCCAGTTTA 
TTTTGTCCTT TCAACTTTCC AACTCAGCCT GTAAATCTGT TTAACCTACC GGGGTTTACT 
AATTTTTCGT CATTTGCACC AAGTATGAAT TTCAGCCCTT TATTTCCTTC TAATTTTGGA 
GATTTTTCTC AAAATATTTC TACACCCACT GAACAGCAGC AACCGTTAGC CCAGAATCCT 
TCAGGGAAAA CGGAATACAT GGCTTTTCCA AAACCTTTTG AAAGTAGTTC TTCTATTGGA 
GCGGAAAAAC AAAGGAATCA AAAACAGCCT GAAGAGGAGG TGGAGAACAG TAGGACACCA 
TGGTTTTATG ACCAAGAAGG TGAAGTAGAA AAGCCATTTC TCAAGACTGG ATTTGCAGTG 
TCTGTAGAGA AAACAACAAA TAGTAATCGC AAAAATCAAC TAGATACCAG TAGGAGAAGA 
CGCCAGTTTG ATGAAGTGTC CTTAGAAAGC TTTAACAGTA TGCTTGACCC AGTAGATCCA 
ACAACAGTAA CTAAAACATT CAAGTCAAGA AAAGCATCTG CACAGGCCAG CCTGGCATCT 
AAAGATAAAA CTCCCAAATC AAAGAGTAAG AAGAGGCATT CTACTCAGCT GAAAAGCAGA 
GTGAAAAATA TCGGGTATGA AAGTGCCAGT GTGTCTAGCA CATGTGAACC TTGCAAAAGT 
AGAAACAGAC ATTCAGCCCA AACTGAAGAG CCTGTTCAAG CAAAAGTACT CAGCAGAAAG 
AATCATGAGC AGCTGGAAAA AGTCATAAGA TGTAGTAGGT CTACAGAAAT ATCTTCAGAA 
ACCGGGAGTG ATTTTTCCAT GTTCGAAGCT TTGCGGGATA CCATTTATTC TGAAGTAGCT 
ACGTTGATTT CTCAAAATGA ATCTCGTCCA CATTTTCTTA TTGAACTCTT CCATGAGCTT 
CAGCTACTTA ATACAGACTA TTTGAGACAG AGGGCCTTAT ATGCATTGCA GGACATAGTA 
TCCAGGCATA TTTCTGAGAA CCATGAAAAA GAAGGAGAAA ATATAAAGTC CGTGAATTCT 
GGTACTTGGA TAGCATCCAA CTCAGAACTT ACTCCCAGTG AAAGCCTTGC TACTACTGAT 
GATGAAACTT TTGAGAAGAA CTTTGAGAGA GAAACACATA AAATAAGTGA GCAAAATGAT 
GCTGATAATG CTAGTGTATC TTCAACTTGT GAGCCTTTTG CAACAGATGT TCTGGGCGAC 
ACTGTGATTC ACTTAGATCA GGCATTAGCC AGAATGAGAG ACTATACGCA TATGAAGATG 
GAGGCTGAAA GTAGTCCAGA CGTCAGGTGC ACCTGCAGGA TCATTGAGGA TGACGATGGT 
GCTGCTGCCA CAACCACGAT TAGTAATAAT TTAGAAGAAA CTCCTGTTAT TGAAAATCAT 
AGTTCACAGC AACCCGTGAG TGAAGTTGCT ACCGTCCCGT GTCCTAGAAT TGATACTCAG 
CAACTGGACC GGCAAATTAA AGCAATTATG AAAGAAGTCA TTCCTTTTTT GAAGGAGCAC 
ATGGATGAAG TATGTTCTTC TCAACTTCTA ACCTCAGTAA GACGCATGGT TTTGGTCCTT 
ACTCAGCAAA ACAATGAGAG CAAAGAGTTT GTAAAGTTCT TTCATAAGCA GCTTGGAAGT 
ATATTACAGG ATTCACTCGC AAAATTTGCT GGCAGAAAAC TGAAGGATTG TGGAGAAGAT 
CTTCTTGTAG AGATATCTGA AGTGTTATTT AATGAATTGG CTTTCTTTAA GCTCATGCAA 
GATTTGGATA ATAATAGTGT AACTGTTAAA CAGAGATGCA AAAGGAAAAT AGAAGCTGCT 
GGAGTGATAC AGTCTTATGC AAAAGAGGCC AAAAGGATTC TTGAAGGAGA TCACAGTTCA 
CCTGCTGGAG AAATTGATGA TGAAGACAAA GACAAGGATG AGACGGAAAC AGTTAAGCAG 
ACTCAAACGT CTGAGGTTTA TGATGGTGAC GGCCCCAAAA ATGTGAGATC TGATGTTTCT 
GATCAAGAGG ATGATGAAGA AAGTGAAGAA TGTCCAGTGT CTATTAATTT GTCCAAAGCT 
GAAACTCAGG CTTTGACTAA TTACGGAAGT GGAGAAGATG AGAATGAAGA TGAAGAAATA 
GAAGAATTTG AAGAGGGACC TGTAGATGTC CAGACGTCAC TTCAGGCTAA CACTGAAACT 
ACAGAAGAAA ATGAACACGA TGATCAGGTT CCACAGCATG ATTTTGAAAA ATCATCAGAA 
AGCAAAAATG TCCCATCAGA ACAAGAACCC ACTACTAGTA AAGGTGACCA GAATAGCACT 
CCTGTGAAGC CCTGTTACCT CAGTATCGTG GAAAATGAGC AACCTTTAAA TAGCCCTGTC 
CAGAAGGACA CTCTCACTAC CATCGACTCA TCCAGTCAGC CTAACCCTTT GCCTTTACCT 
TTAACTGAAA TTGAAACCTT GGTGCCTAGA GTCAAAGAAG TGAAATCTGC TCAGGAGACT 
CCTGAAAGTT CTTTGGCTGG AAGTCCTGAT ACTGAATCTC CAGTGTTAGT GAATGACTAT 
GAAGCAGAAT CTGGTAACAT AAGTCAAAAA TCTGATGAAG AAGACTTTGT GAAAGTTGAA 
GATTTACCAC TTAAACTGAC AATATATTCA GAGGCAGATC TAAGGAAGAA AATGGCCGAA 
GAAGAACAGA AAAACCACTT ATCTGGTGAA ATATGTGCAA TGCAGACTGA AGAATTAGCT 
GGGAATTCTC AGACACTAAA AGAACCTGAA ACAGTGGGAG CCCAAAGTAC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( XM_025279520.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025135305.1 Bubalus bubalis pericentriolar material 1 (PCM1), transcript variant X21, mRNA.

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CloneID	OBu42309
Clone ID Related Accession (Same CDS sequence)	XM_025279520.1
Accession Version	XM_025279520.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6129bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	pericentriolar material 1 protein isoform X20
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037545.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG GAGGAGGTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
AGCAATGAGA GTGTTGATGA CCGACTCAAC AATATGGATT GGGGCAGCCA GCAGAAGAAG 
GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA 
ACTAACGATA TTTCTCCGGA GTCATCACCG GGAGTTGGAA GACGAAGAAC AAAGACTTCA 
CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TCCCCGAGCA GGCAGAACTA 
GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCTCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCCAC TGTAAGTCCT 
CAAAAGAGAG AAATGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAGATTCGTG AGTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGACCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTCACT 
CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT CCAGAAGATT 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCGGT CTGAGGCTTC AGATGCCACG 
GCCAGAGATC CTCAGCAGGA GCCTATGGAA GAGATAGAAA ATTTGAAGAA ACAACATGAT 
TTATTAAAAA GGATGTTACA GCAGCAGGAG CAGCTGAGAG CTCTACAAGG ACGACAAGCT 
GCTCTGCTTG CCCTGCAGCA TAAAGCTGAG CAGGCTATAG CTGTGATGGA TGATTCTGAA 
AAGGTTGCGG AGACAACTCC TGGCCATCAT ACTGTACCAG GCAGACAGCC GGCTCATTCA 
CCTTTGCATC AGAGAGTACC CATAAGAGAA ACTACAGGTA GCCTATCTGG AGTCAGTATC 
ACCTCTGAAC TAAACGAAGA ATTGAATGAT TTAATTCAGC ATTTTCATAA TCAGCTTCGT 
GATTCTCAGC CTCCAGCTGT TCCAGACAAT AGAAGACAAG CAGAAAGTCT TTCATTAACT 
AGGGAGATTT CTCAGAGCAG AAATCCTTCA GTTTCAGAGC ATTTACCTGA TGAGAAAGTC 
CAACTTTTTA GCAAGATGAG AGTGTTACAA GAAAAGAAAC AAAAAATGGA CAAACTTCTT 
GGAGAACTTC ATACACTTCG AGATCAGCAT TTGAACAATT CATCATTTGT GCCTTCTTCA 
GCCTCTCCAC AAAGGAGTGT GGATCAGAGA AGTACAACCA CAGCTCCTTC TGCTCCTGTA 
GTCTTAACAC CAGTGGTCAA TGGAGAATCC AATAACCTCA CGCCATCTGT TCCTTATCCT 
GCTGCTTCTC TAGTTTCTCA GAATCAGAAT GAAAATGAAG GCCACCTAAA TCCAACTGAA 
AAACTCCAGA AGTTAAATGA AGTTCGAAAG AGGTTGAATG AACTAAGAGA ATTAGTTCAT 
TATTATGAGC AAACATCAGA TATGATGACA GATGCTGTGA ATGAAAACAC AAAAGAAGAA 
GAAACTGAAG AATCAGAATA TGATTCGGAG CATGAAAACT CGGAACCTGT TACTAACATT 
CGAAATCCAC AAGTGGCTGC CACCTGGAAC GAAGTAAATA GTAATTCTAA TGCACAGTGT 
GTTTCCAACA ATAGAGATGG GCGAGCAGTT AATTCTAACT GTGAAATTAA CAACAGGTCT 
GCTGCCAACA TAAGGGCTCT AAACATGCCT CCTCTTTCAG CAGATTGTCG ATACAATAGA 
GAAGGAGAAC AGGAGATGCC TGTTGCACAA GGTCATGAGG AGGAGGAGGA GGAGGAGGAG 
GAAGCAGAAG ATGAGGCCCT CAGTGGAGCT TCACTGTCTA GTCACAGCAG CAGCCTGATC 
ATTGAGACTC CTGAAGATGC TGAATTTGAA CATAAGATCC AGAAGCTTAT GGCTGCAAAA 
CTGAAACTTA GACACTTACA GAACCTTGTA GCCATGGTGC AGGACGATGA TGCAGCCAAT 
CAAGGAGTTA CTTCTGTGGA GGATTTCTTC CCAGCAGAAG ACACCAAACA AAATGCAAAT 
AACACTAGAG GAAATGCCAA TAAAACACAG AAAGATGCTG GAGTAAATGA AAAGGCAAGG 
GAGAAATTTT ATGAGGCTAA ACTACAGCAG CAACAGAGAG AACTCAAACA ATTGCAGGAA 
GAAAGAAAGA AACTGATTGA GATTCAAGAG AAAATTCAAG AATTGCAAAA GGCATGTCCT 
AACCTACAGC TGTCAGCTGC TAGTGTGGGT AATTGTCCCA CAAAAACACA TATGCCAGCT 
GTTACTTCAT CCCCAACCGT TAATGAAAAT GAAGCCAGTA CAAACAAATC TGGTTTTGAG 
CCTCATGATT CTTCAGTGGT AGATAATGAG GTATGGTCAG ATATGCGAAG ACATGAAATG 
TTAAGGGAGG AGCTACGACA GAGAAGAAAG CAGCTGGAAG CTCTGATGGC TGAACATCAG 
AGGAGGCAAG GTCTGGCTGA AACTGCATCT CCAGTGGCTG TTTCACTGAG AAGTGATGGA 
TCTGAGAACC TGTGTACACC TCAGCAAAGC AGAACAGAAA AAACAATGGC AACTTGGGGA 
GGTTCTACCC AGTGTGCCCT AGATGAAGAA GGAGATGAAG ATGGTTACCT TTCTGAAGGA 
ATTATTCGGA CAGACGAAGA GGAGGAAGAG GAGGAGCAAG ATGCCAGTTC CAATGATAAC 
TTTCATATGT ATCCTCCAAA CAGTGCAAAT CATAATTCTT ACAATGTAAA GGAAACTAAA 
AATAGGTGGA AGAACAATCG CCCTTTTTCA GCAGATGGAA ATTATCGTCC TTTAGCCAAG 
ACAAGACAGC AGAATATCAG CATGCAGCGT CAGGAAAACC TTCGCTGGGT GTCCGAACTC 
TCTTATGTAG AAGAGAAAGA ACAATGGCAA GAACAAATTA ATCAGCTAAA GAAACAACTT 
GATTTCAGTG TCAATATTTG TCAGACTTTA ATGCAAGACC AGCAGACTCT GTCTTGTCTG 
CTACAAACTC TTCTCACGAG TCCTTATAGT GTTATGCCCA GCAGTGTTGC ATCCCCGCAA 
GTGCACCTTA TAATGCACCA GTTGAATCAG TGCTATACCC AGCTAACGTG GCAGCAGAAC 
AATGTCCAGA GGTTGAAACA AATGCTCAGT GAACTCATGC GCCAACAAAA TCAGCATCCA 
GAAAAACCTG CAAGCAAGGA AGGAGGCAGT GGTACATCAC ACCCTCCTTC TCCCAGTTTA 
TTTTGTCCTT TCAACTTTCC AACTCAGCCT GTAAATCTGT TTAACCTACC GGGGTTTACT 
AATTTTTCGT CATTTGCACC AAGTATGAAT TTCAGCCCTT TATTTCCTTC TAATTTTGGA 
GATTTTTCTC AAAATATTTC TACACCCACT GAACAGCAGC AACCGTTAGC CCAGAATCCT 
TCAGGGAAAA CGGAATACAT GGCTTTTCCA AAACCTTTTG AAAGTAGTTC TTCTATTGGA 
GCGGAAAAAC AAAGGAATCA AAAACAGCCT GAAGAGGAGG TGGAGAACAG TAGGACACCA 
TGGTTTTATG ACCAAGAAGG TGAAGTAGAA AAGCCATTTC TCAAGACTGG ATTTGCAGTG 
TCTGTAGAGA AAACAACAAA TAGTAATCGC AAAAATCAAC TAGATACCAG TAGGAGAAGA 
CGCCAGTTTG ATGAAGTGTC CTTAGAAAGC TTTAACAGTA TGCTTGACCC AGTAGATCCA 
ACAACAGTAA CTAAAACATT CAAGTCAAGA AAAGCATCTG CACAGGCCAG CCTGGCATCT 
AAAGATAAAA CTCCCAAATC AAAGAGTAAG AAGAGGCATT CTACTCAGCT GAAAAGCAGA 
GTGAAAAATA TCGAAACCGG GAGTGATTTT TCCATGTTCG AAGCTTTGCG GGATACCATT 
TATTCTGAAG TAGCTACGTT GATTTCTCAA AATGAATCTC GTCCACATTT TCTTATTGAA 
CTCTTCCATG AGCTTCAGCT ACTTAATACA GACTATTTGA GACAGAGGGC CTTATATGCA 
TTGCAGGACA TAGTATCCAG GCATATTTCT GAGAACCATG AAAAAGAAGG AGAAAATATA 
AAGTCCGTGA ATTCTGGTAC TTGGATAGCA TCCAACTCAG AACTTACTCC CAGTGAAAGC 
CTTGCTACTA CTGATGATGA AACTTTTGAG AAGAACTTTG AGAGAGAAAC ACATAAAATA 
AGTGAGCAAA ATGATGCTGA TAATGCTAGT GTATCTTCAA CTTGTGAGCC TTTTGCAACA 
GATGTTCTGG GCGACACTGT GATTCACTTA GATCAGGCAT TAGCCAGAAT GAGAGACTAT 
ACGCATATGA AGATGGAGGC TGAAAGTAGT CCAGACGTCA GGTGCACCTG CAGGATCATT 
GAGGATGACG ATGGTGCTGC TGCCACAACC ACGATTAGTA ATAATTTAGA AGAAACTCCT 
GTTATTGAAA ATCATAGTTC ACAGCAACCC GTGAGTGAAG TTGCTACCGT CCCGTGTCCT 
AGAATTGATA CTCAGCAACT GGACCGGCAA ATTAAAGCAA TTATGAAAGA AGTCATTCCT 
TTTTTGAAGG AGCACATGGA TGAAGTATGT TCTTCTCAAC TTCTAACCTC AGTAAGACGC 
ATGGTTTTGG TCCTTACTCA GCAAAACAAT GAGAGCAAAG AGTTTGTAAA GTTCTTTCAT 
AAGCAGCTTG GAAGTATATT ACAGGATTCA CTCGCAAAAT TTGCTGGCAG AAAACTGAAG 
GATTGTGGAG AAGATCTTCT TGTAGAGATA TCTGAAGTGT TATTTAATGA ATTGGCTTTC 
TTTAAGCTCA TGCAAGATTT GGATAATAAT AGTGTAACTG TTAAACAGAG ATGCAAAAGG 
AAAATAGAAG CTGCTGGAGT GATACAGTCT TATGCAAAAG AGGCCAAAAG GATTCTTGAA 
GGAGATCACA GTTCACCTGC TGGAGAAATT GATGATGAAG ACAAAGACAA GGATGAGACG 
GAAACAGTTA AGCAGACTCA AACGTCTGAG GTTTATGATG GTGACGGCCC CAAAAATGTG 
AGATCTGATG TTTCTGATCA AGAGGATGAT GAAGAAAGTG AAGAATGTCC AGTGTCTATT 
AATTTGTCCA AAGCTGAAAC TCAGGCTTTG ACTAATTACG GAAGTGGAGA AGATGAGAAT 
GAAGATGAAG AAATAGAAGA ATTTGAAGAG GGACCTGTAG ATGTCCAGAC GTCACTTCAG 
GCTAACACTG AAACTACAGA AGAAAATGAA CACGATGATC AGGTTCCACA GCATGATTTT 
GAAAAATCAT CAGAAAGCAA AAATGTCCCA TCAGAACAAG AACCCACTAC TAGTAAAGGT 
GACCAGAATA GCACTCCTGT GAAGCCCTGT TACCTCAGTA TCGTGGAAAA TGAGCAACCT 
TTAAATAGCC CTGTCCAGAA GGACACTCTC ACTACCATCG ACTCATCCAG TCAGCCTAAC 
CCTTTGCCTT TACCTTTAAC TGAAATTGAA ACCTTGGTGC CTAGAGTCAA AGAAGTGAAA 
TCTGCTCAGG AGACTCCTGA AAGTTCTTTG GCTGGAAGTC CTGATACTGA ATCTCCAGTG 
TTAGTGAATG ACTATGAAGC AGAATCTGGT AACATAAGTC AAAAATCTGA TGAAGAAGAC 
TTTGTGAAAG TTGAAGATTT ACCACTTAAA CTGACAATAT ATTCAGAGGC AGATCTAAGG 
AAGAAAATGG CCGAAGAAGA ACAGAAAAAC CACTTATCTG GTGAAATATG TGCAATGCAG 
ACTGAAGAAT TAGCTGGGAA TTCTCAGACA CTAAAAGAAC CTGAAACAGT GGGAGCCCAA 
AGTACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025135305.1
CDS	392..6520
Translation	MATGGGPFEEGVNDQDLPNWSNESVDDRLNNMDWGSQQKKANRSSEKNKRKFGVESDKRVTNDISPESSPGVGRRRTKTSLTFPHSRYMTQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDATVSPQKREMIGSAQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIREYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLQKILARENEEEDVRTIDSAVGSGSVAESTSLNIDVRSEASDATARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSEKVAETTPGHHTVPGRQPAHSPLHQRVPIRETTGSLSGVSITSELNEELNDLIQHFHNQLRDSQPPAVPDNRRQAESLSLTREISQSRNPSVSEHLPDEKVQLFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSFVPSSASPQRSVDQRSTTTAPSAPVVLTPVVNGESNNLTPSVPYPAASLVSQNQNENEGHLNPTEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENTKEEETEESEYDSEHENSEPVTNIRNPQVAATWNEVNSNSNAQCVSNNRDGRAVNSNCEINNRSAANIRALNMPPLSADCRYNREGEQEMPVAQGHEEEEEEEEEAEDEALSGASLSSHSSSLIIETPEDAEFEHKIQKLMAAKLKLRHLQNLVAMVQDDDAANQGVTSVEDFFPAEDTKQNANNTRGNANKTQKDAGVNEKAREKFYEAKLQQQQRELKQLQEERKKLIEIQEKIQELQKACPNLQLSAASVGNCPTKTHMPAVTSSPTVNENEASTNKSGFEPHDSSVVDNEVWSDMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETASPVAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGIIRTDEEEEEEEQDASSNDNFHMYPPNSANHNSYNVKETKNRWKNNRPFSADGNYRPLAKTRQQNISMQRQENLRWVSELSYVEEKEQWQEQINQLKKQLDFSVNICQTLMQDQQTLSCLLQTLLTSPYSVMPSSVASPQVHLIMHQLNQCYTQLTWQQNNVQRLKQMLSELMRQQNQHPEKPASKEGGSGTSHPPSPSLFCPFNFPTQPVNLFNLPGFTNFSSFAPSMNFSPLFPSNFGDFSQNISTPTEQQQPLAQNPSGKTEYMAFPKPFESSSSIGAEKQRNQKQPEEEVENSRTPWFYDQEGEVEKPFLKTGFAVSVEKTTNSNRKNQLDTSRRRRQFDEVSLESFNSMLDPVDPTTVTKTFKSRKASAQASLASKDKTPKSKSKKRHSTQLKSRVKNIETGSDFSMFEALRDTIYSEVATLISQNESRPHFLIELFHELQLLNTDYLRQRALYALQDIVSRHISENHEKEGENIKSVNSGTWIASNSELTPSESLATTDDETFEKNFERETHKISEQNDADNASVSSTCEPFATDVLGDTVIHLDQALARMRDYTHMKMEAESSPDVRCTCRIIEDDDGAAATTTISNNLEETPVIENHSSQQPVSEVATVPCPRIDTQQLDRQIKAIMKEVIPFLKEHMDEVCSSQLLTSVRRMVLVLTQQNNESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSVTVKQRCKRKIEAAGVIQSYAKEAKRILEGDHSSPAGEIDDEDKDKDETETVKQTQTSEVYDGDGPKNVRSDVSDQEDDEESEECPVSINLSKAETQALTNYGSGEDENEDEEIEEFEEGPVDVQTSLQANTETTEENEHDDQVPQHDFEKSSESKNVPSEQEPTTSKGDQNSTPVKPCYLSIVENEQPLNSPVQKDTLTTIDSSSQPNPLPLPLTEIETLVPRVKEVKSAQETPESSLAGSPDTESPVLVNDYEAESGNISQKSDEEDFVKVEDLPLKLTIYSEADLRKKMAEEEQKNHLSGEICAMQTEELAGNSQTLKEPETVGAQST

Target ORF information:

RefSeq Version	XM_025279520.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis pericentriolar material 1 (PCM1), transcript variant X21, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025279520.1

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
AGCAATGAGA GTGTTGATGA CCGACTCAAC AATATGGATT GGGGCAGCCA GCAGAAGAAG 
GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA 
ACTAACGATA TTTCTCCGGA GTCATCACCG GGAGTTGGAA GACGAAGAAC AAAGACTTCA 
CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TCCCCGAGCA GGCAGAACTA 
GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCTCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCCAC TGTAAGTCCT 
CAAAAGAGAG AAATGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAGATTCGTG AGTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGACCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTCACT 
CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT CCAGAAGATT 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCGGT CTGAGGCTTC AGATGCCACG 
GCCAGAGATC CTCAGCAGGA GCCTATGGAA GAGATAGAAA ATTTGAAGAA ACAACATGAT 
TTATTAAAAA GGATGTTACA GCAGCAGGAG CAGCTGAGAG CTCTACAAGG ACGACAAGCT 
GCTCTGCTTG CCCTGCAGCA TAAAGCTGAG CAGGCTATAG CTGTGATGGA TGATTCTGAA 
AAGGTTGCGG AGACAACTCC TGGCCATCAT ACTGTACCAG GCAGACAGCC GGCTCATTCA 
CCTTTGCATC AGAGAGTACC CATAAGAGAA ACTACAGGTA GCCTATCTGG AGTCAGTATC 
ACCTCTGAAC TAAACGAAGA ATTGAATGAT TTAATTCAGC ATTTTCATAA TCAGCTTCGT 
GATTCTCAGC CTCCAGCTGT TCCAGACAAT AGAAGACAAG CAGAAAGTCT TTCATTAACT 
AGGGAGATTT CTCAGAGCAG AAATCCTTCA GTTTCAGAGC ATTTACCTGA TGAGAAAGTC 
CAACTTTTTA GCAAGATGAG AGTGTTACAA GAAAAGAAAC AAAAAATGGA CAAACTTCTT 
GGAGAACTTC ATACACTTCG AGATCAGCAT TTGAACAATT CATCATTTGT GCCTTCTTCA 
GCCTCTCCAC AAAGGAGTGT GGATCAGAGA AGTACAACCA CAGCTCCTTC TGCTCCTGTA 
GTCTTAACAC CAGTGGTCAA TGGAGAATCC AATAACCTCA CGCCATCTGT TCCTTATCCT 
GCTGCTTCTC TAGTTTCTCA GAATCAGAAT GAAAATGAAG GCCACCTAAA TCCAACTGAA 
AAACTCCAGA AGTTAAATGA AGTTCGAAAG AGGTTGAATG AACTAAGAGA ATTAGTTCAT 
TATTATGAGC AAACATCAGA TATGATGACA GATGCTGTGA ATGAAAACAC AAAAGAAGAA 
GAAACTGAAG AATCAGAATA TGATTCGGAG CATGAAAACT CGGAACCTGT TACTAACATT 
CGAAATCCAC AAGTGGCTGC CACCTGGAAC GAAGTAAATA GTAATTCTAA TGCACAGTGT 
GTTTCCAACA ATAGAGATGG GCGAGCAGTT AATTCTAACT GTGAAATTAA CAACAGGTCT 
GCTGCCAACA TAAGGGCTCT AAACATGCCT CCTCTTTCAG CAGATTGTCG ATACAATAGA 
GAAGGAGAAC AGGAGATGCC TGTTGCACAA GGTCATGAGG AGGAGGAGGA GGAGGAGGAG 
GAAGCAGAAG ATGAGGCCCT CAGTGGAGCT TCACTGTCTA GTCACAGCAG CAGCCTGATC 
ATTGAGACTC CTGAAGATGC TGAATTTGAA CATAAGATCC AGAAGCTTAT GGCTGCAAAA 
CTGAAACTTA GACACTTACA GAACCTTGTA GCCATGGTGC AGGACGATGA TGCAGCCAAT 
CAAGGAGTTA CTTCTGTGGA GGATTTCTTC CCAGCAGAAG ACACCAAACA AAATGCAAAT 
AACACTAGAG GAAATGCCAA TAAAACACAG AAAGATGCTG GAGTAAATGA AAAGGCAAGG 
GAGAAATTTT ATGAGGCTAA ACTACAGCAG CAACAGAGAG AACTCAAACA ATTGCAGGAA 
GAAAGAAAGA AACTGATTGA GATTCAAGAG AAAATTCAAG AATTGCAAAA GGCATGTCCT 
AACCTACAGC TGTCAGCTGC TAGTGTGGGT AATTGTCCCA CAAAAACACA TATGCCAGCT 
GTTACTTCAT CCCCAACCGT TAATGAAAAT GAAGCCAGTA CAAACAAATC TGGTTTTGAG 
CCTCATGATT CTTCAGTGGT AGATAATGAG GTATGGTCAG ATATGCGAAG ACATGAAATG 
TTAAGGGAGG AGCTACGACA GAGAAGAAAG CAGCTGGAAG CTCTGATGGC TGAACATCAG 
AGGAGGCAAG GTCTGGCTGA AACTGCATCT CCAGTGGCTG TTTCACTGAG AAGTGATGGA 
TCTGAGAACC TGTGTACACC TCAGCAAAGC AGAACAGAAA AAACAATGGC AACTTGGGGA 
GGTTCTACCC AGTGTGCCCT AGATGAAGAA GGAGATGAAG ATGGTTACCT TTCTGAAGGA 
ATTATTCGGA CAGACGAAGA GGAGGAAGAG GAGGAGCAAG ATGCCAGTTC CAATGATAAC 
TTTCATATGT ATCCTCCAAA CAGTGCAAAT CATAATTCTT ACAATGTAAA GGAAACTAAA 
AATAGGTGGA AGAACAATCG CCCTTTTTCA GCAGATGGAA ATTATCGTCC TTTAGCCAAG 
ACAAGACAGC AGAATATCAG CATGCAGCGT CAGGAAAACC TTCGCTGGGT GTCCGAACTC 
TCTTATGTAG AAGAGAAAGA ACAATGGCAA GAACAAATTA ATCAGCTAAA GAAACAACTT 
GATTTCAGTG TCAATATTTG TCAGACTTTA ATGCAAGACC AGCAGACTCT GTCTTGTCTG 
CTACAAACTC TTCTCACGAG TCCTTATAGT GTTATGCCCA GCAGTGTTGC ATCCCCGCAA 
GTGCACCTTA TAATGCACCA GTTGAATCAG TGCTATACCC AGCTAACGTG GCAGCAGAAC 
AATGTCCAGA GGTTGAAACA AATGCTCAGT GAACTCATGC GCCAACAAAA TCAGCATCCA 
GAAAAACCTG CAAGCAAGGA AGGAGGCAGT GGTACATCAC ACCCTCCTTC TCCCAGTTTA 
TTTTGTCCTT TCAACTTTCC AACTCAGCCT GTAAATCTGT TTAACCTACC GGGGTTTACT 
AATTTTTCGT CATTTGCACC AAGTATGAAT TTCAGCCCTT TATTTCCTTC TAATTTTGGA 
GATTTTTCTC AAAATATTTC TACACCCACT GAACAGCAGC AACCGTTAGC CCAGAATCCT 
TCAGGGAAAA CGGAATACAT GGCTTTTCCA AAACCTTTTG AAAGTAGTTC TTCTATTGGA 
GCGGAAAAAC AAAGGAATCA AAAACAGCCT GAAGAGGAGG TGGAGAACAG TAGGACACCA 
TGGTTTTATG ACCAAGAAGG TGAAGTAGAA AAGCCATTTC TCAAGACTGG ATTTGCAGTG 
TCTGTAGAGA AAACAACAAA TAGTAATCGC AAAAATCAAC TAGATACCAG TAGGAGAAGA 
CGCCAGTTTG ATGAAGTGTC CTTAGAAAGC TTTAACAGTA TGCTTGACCC AGTAGATCCA 
ACAACAGTAA CTAAAACATT CAAGTCAAGA AAAGCATCTG CACAGGCCAG CCTGGCATCT 
AAAGATAAAA CTCCCAAATC AAAGAGTAAG AAGAGGCATT CTACTCAGCT GAAAAGCAGA 
GTGAAAAATA TCGAAACCGG GAGTGATTTT TCCATGTTCG AAGCTTTGCG GGATACCATT 
TATTCTGAAG TAGCTACGTT GATTTCTCAA AATGAATCTC GTCCACATTT TCTTATTGAA 
CTCTTCCATG AGCTTCAGCT ACTTAATACA GACTATTTGA GACAGAGGGC CTTATATGCA 
TTGCAGGACA TAGTATCCAG GCATATTTCT GAGAACCATG AAAAAGAAGG AGAAAATATA 
AAGTCCGTGA ATTCTGGTAC TTGGATAGCA TCCAACTCAG AACTTACTCC CAGTGAAAGC 
CTTGCTACTA CTGATGATGA AACTTTTGAG AAGAACTTTG AGAGAGAAAC ACATAAAATA 
AGTGAGCAAA ATGATGCTGA TAATGCTAGT GTATCTTCAA CTTGTGAGCC TTTTGCAACA 
GATGTTCTGG GCGACACTGT GATTCACTTA GATCAGGCAT TAGCCAGAAT GAGAGACTAT 
ACGCATATGA AGATGGAGGC TGAAAGTAGT CCAGACGTCA GGTGCACCTG CAGGATCATT 
GAGGATGACG ATGGTGCTGC TGCCACAACC ACGATTAGTA ATAATTTAGA AGAAACTCCT 
GTTATTGAAA ATCATAGTTC ACAGCAACCC GTGAGTGAAG TTGCTACCGT CCCGTGTCCT 
AGAATTGATA CTCAGCAACT GGACCGGCAA ATTAAAGCAA TTATGAAAGA AGTCATTCCT 
TTTTTGAAGG AGCACATGGA TGAAGTATGT TCTTCTCAAC TTCTAACCTC AGTAAGACGC 
ATGGTTTTGG TCCTTACTCA GCAAAACAAT GAGAGCAAAG AGTTTGTAAA GTTCTTTCAT 
AAGCAGCTTG GAAGTATATT ACAGGATTCA CTCGCAAAAT TTGCTGGCAG AAAACTGAAG 
GATTGTGGAG AAGATCTTCT TGTAGAGATA TCTGAAGTGT TATTTAATGA ATTGGCTTTC 
TTTAAGCTCA TGCAAGATTT GGATAATAAT AGTGTAACTG TTAAACAGAG ATGCAAAAGG 
AAAATAGAAG CTGCTGGAGT GATACAGTCT TATGCAAAAG AGGCCAAAAG GATTCTTGAA 
GGAGATCACA GTTCACCTGC TGGAGAAATT GATGATGAAG ACAAAGACAA GGATGAGACG 
GAAACAGTTA AGCAGACTCA AACGTCTGAG GTTTATGATG GTGACGGCCC CAAAAATGTG 
AGATCTGATG TTTCTGATCA AGAGGATGAT GAAGAAAGTG AAGAATGTCC AGTGTCTATT 
AATTTGTCCA AAGCTGAAAC TCAGGCTTTG ACTAATTACG GAAGTGGAGA AGATGAGAAT 
GAAGATGAAG AAATAGAAGA ATTTGAAGAG GGACCTGTAG ATGTCCAGAC GTCACTTCAG 
GCTAACACTG AAACTACAGA AGAAAATGAA CACGATGATC AGGTTCCACA GCATGATTTT 
GAAAAATCAT CAGAAAGCAA AAATGTCCCA TCAGAACAAG AACCCACTAC TAGTAAAGGT 
GACCAGAATA GCACTCCTGT GAAGCCCTGT TACCTCAGTA TCGTGGAAAA TGAGCAACCT 
TTAAATAGCC CTGTCCAGAA GGACACTCTC ACTACCATCG ACTCATCCAG TCAGCCTAAC 
CCTTTGCCTT TACCTTTAAC TGAAATTGAA ACCTTGGTGC CTAGAGTCAA AGAAGTGAAA 
TCTGCTCAGG AGACTCCTGA AAGTTCTTTG GCTGGAAGTC CTGATACTGA ATCTCCAGTG 
TTAGTGAATG ACTATGAAGC AGAATCTGGT AACATAAGTC AAAAATCTGA TGAAGAAGAC 
TTTGTGAAAG TTGAAGATTT ACCACTTAAA CTGACAATAT ATTCAGAGGC AGATCTAAGG 
AAGAAAATGG CCGAAGAAGA ACAGAAAAAC CACTTATCTG GTGAAATATG TGCAATGCAG 
ACTGAAGAAT TAGCTGGGAA TTCTCAGACA CTAAAAGAAC CTGAAACAGT GGGAGCCCAA 
AGTACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( XM_025279526.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025135311.1 Bubalus bubalis pericentriolar material 1 (PCM1), transcript variant X22, mRNA.

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CloneID	OBu42310
Clone ID Related Accession (Same CDS sequence)	XM_025279526.1
Accession Version	XM_025279526.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6126bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	pericentriolar material 1 protein isoform X21
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037545.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG GAGGAGGTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
AGCAATGAGA GTGTTGATGA CCGACTCAAC AATATGGATT GGGGCAGCCA GCAGAAGAAG 
GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA 
ACTAACGATA TTTCTCCGGA GTCATCACCG GGAGTTGGAA GACGAAGAAC AAAGACTTCA 
CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TCCCCGAGCA GGCAGAACTA 
GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCTCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCCAC TGTAAGTCCT 
CAAAAGAGAG AAATGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAGATTCGTG AGTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGACCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTCACT 
CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT CCAGAAGATT 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCGGT CTGAGGCTTC AGATGCCACG 
GCCAGAGATC CTCAGCAGGA GCCTATGGAA GAGATAGAAA ATTTGAAGAA ACAACATGAT 
TTATTAAAAA GGATGTTACA GCAGCAGGAG CAGCTGAGAG CTCTACAAGG ACGACAAGCT 
GCTCTGCTTG CCCTGCAGCA TAAAGCTGAG CAGGCTATAG CTGTGATGGA TGATTCTGAA 
AAGGTTGCGG AGACAACTCC TGGCCATCAT ACTGTACCAG GCAGACAGCC GGCTCATTCA 
CCTTTGCATC AGAGAGTACC CATAAGAGAA ACTACAGGTA GCCTATCTGG AGTCAGTATC 
ACCTCTGAAC TAAACGAAGA ATTGAATGAT TTAATTCAGC ATTTTCATAA TCAGCTTCGT 
GATTCTCAGC CTCCAGCTGT TCCAGACAAT AGAAGACAAG CAGAAAGTCT TTCATTAACT 
AGGGAGATTT CTCAGAGCAG AAATCCTTCA GTTTCAGAGC ATTTACCTGA TGAGAAAGTC 
CAACTTTTTA GCAAGATGAG AGTGTTACAA GAAAAGAAAC AAAAAATGGA CAAACTTCTT 
GGAGAACTTC ATACACTTCG AGATCAGCAT TTGAACAATT CATCATTTGT GCCTTCTTCA 
GCCTCTCCAC AAAGGAGTGT GGATCAGAGA AGTACAACCA CAGCTCCTTC TGCTCCTGTA 
GTCTTAACAC CAGTGGTCAA TGGAGAATCC AATAACCTCA CGCCATCTGT TCCTTATCCT 
GCTGCTTCTC TAGTTTCTCA GAATCAGAAT GAAAATGAAG GCCACCTAAA TCCAACTGAA 
AAACTCCAGA AGTTAAATGA AGTTCGAAAG AGGTTGAATG AACTAAGAGA ATTAGTTCAT 
TATTATGAGC AAACATCAGA TATGATGACA GATGCTGTGA ATGAAAACAC AAAAGAAGAA 
GAAACTGAAG AATCAGAATA TGATTCGGAG CATGAAAACT CGGAACCTGT TACTAACATT 
CGAAATCCAC AAGTGGCTGC CACCTGGAAC GAAGTAAATA GTAATTCTAA TGCACAGTGT 
GTTTCCAACA ATAGAGATGG GCGAGCAGTT AATTCTAACT GTGAAATTAA CAACAGGTCT 
GCTGCCAACA TAAGGGCTCT AAACATGCCT CCTCTTTCAG ATTGTCGATA CAATAGAGAA 
GGAGAACAGG AGATGCCTGT TGCACAAGGT CATGAGGAGG AGGAGGAGGA GGAGGAGGAA 
GCAGAAGATG AGGCCCTCAG TGGAGCTTCA CTGTCTAGTC ACAGCAGCAG CCTGATCATT 
GAGACTCCTG AAGATGCTGA ATTTGAACAT AAGATCCAGA AGCTTATGGC TGCAAAACTG 
AAACTTAGAC ACTTACAGAA CCTTGTAGCC ATGGTGCAGG ACGATGATGC AGCCAATCAA 
GGAGTTACTT CTGTGGAGGA TTTCTTCCCA GCAGAAGACA CCAAACAAAA TGCAAATAAC 
ACTAGAGGAA ATGCCAATAA AACACAGAAA GATGCTGGAG TAAATGAAAA GGCAAGGGAG 
AAATTTTATG AGGCTAAACT ACAGCAGCAA CAGAGAGAAC TCAAACAATT GCAGGAAGAA 
AGAAAGAAAC TGATTGAGAT TCAAGAGAAA ATTCAAGAAT TGCAAAAGGC ATGTCCTAAC 
CTACAGCTGT CAGCTGCTAG TGTGGGTAAT TGTCCCACAA AAACACATAT GCCAGCTGTT 
ACTTCATCCC CAACCGTTAA TGAAAATGAA GCCAGTACAA ACAAATCTGG TTTTGAGCCT 
CATGATTCTT CAGTGGTAGA TAATGAGGTA TGGTCAGATA TGCGAAGACA TGAAATGTTA 
AGGGAGGAGC TACGACAGAG AAGAAAGCAG CTGGAAGCTC TGATGGCTGA ACATCAGAGG 
AGGCAAGGTC TGGCTGAAAC TGCATCTCCA GTGGCTGTTT CACTGAGAAG TGATGGATCT 
GAGAACCTGT GTACACCTCA GCAAAGCAGA ACAGAAAAAA CAATGGCAAC TTGGGGAGGT 
TCTACCCAGT GTGCCCTAGA TGAAGAAGGA GATGAAGATG GTTACCTTTC TGAAGGAATT 
ATTCGGACAG ACGAAGAGGA GGAAGAGGAG GAGCAAGATG CCAGTTCCAA TGATAACTTT 
CATATGTATC CTCCAAACAG TGCAAATCAT AATTCTTACA ATGTAAAGGA AACTAAAAAT 
AGGTGGAAGA ACAATCGCCC TTTTTCAGCA GATGGAAATT ATCGTCCTTT AGCCAAGACA 
AGACAGCAGA ATATCAGCAT GCAGCGTCAG GAAAACCTTC GCTGGGTGTC CGAACTCTCT 
TATGTAGAAG AGAAAGAACA ATGGCAAGAA CAAATTAATC AGCTAAAGAA ACAACTTGAT 
TTCAGTGTCA ATATTTGTCA GACTTTAATG CAAGACCAGC AGACTCTGTC TTGTCTGCTA 
CAAACTCTTC TCACGAGTCC TTATAGTGTT ATGCCCAGCA GTGTTGCATC CCCGCAAGTG 
CACCTTATAA TGCACCAGTT GAATCAGTGC TATACCCAGC TAACGTGGCA GCAGAACAAT 
GTCCAGAGGT TGAAACAAAT GCTCAGTGAA CTCATGCGCC AACAAAATCA GCATCCAGAA 
AAACCTGCAA GCAAGGAAGG AGGCAGTGGT ACATCACACC CTCCTTCTCC CAGTTTATTT 
TGTCCTTTCA ACTTTCCAAC TCAGCCTGTA AATCTGTTTA ACCTACCGGG GTTTACTAAT 
TTTTCGTCAT TTGCACCAAG TATGAATTTC AGCCCTTTAT TTCCTTCTAA TTTTGGAGAT 
TTTTCTCAAA ATATTTCTAC ACCCACTGAA CAGCAGCAAC CGTTAGCCCA GAATCCTTCA 
GGGAAAACGG AATACATGGC TTTTCCAAAA CCTTTTGAAA GTAGTTCTTC TATTGGAGCG 
GAAAAACAAA GGAATCAAAA ACAGCCTGAA GAGGAGGTGG AGAACAGTAG GACACCATGG 
TTTTATGACC AAGAAGGTGA AGTAGAAAAG CCATTTCTCA AGACTGGATT TGCAGTGTCT 
GTAGAGAAAA CAACAAATAG TAATCGCAAA AATCAACTAG ATACCAGTAG GAGAAGACGC 
CAGTTTGATG AAGTGTCCTT AGAAAGCTTT AACAGTATGC TTGACCCAGT AGATCCAACA 
ACAGTAACTA AAACATTCAA GTCAAGAAAA GCATCTGCAC AGGCCAGCCT GGCATCTAAA 
GATAAAACTC CCAAATCAAA GAGTAAGAAG AGGCATTCTA CTCAGCTGAA AAGCAGAGTG 
AAAAATATCG AAACCGGGAG TGATTTTTCC ATGTTCGAAG CTTTGCGGGA TACCATTTAT 
TCTGAAGTAG CTACGTTGAT TTCTCAAAAT GAATCTCGTC CACATTTTCT TATTGAACTC 
TTCCATGAGC TTCAGCTACT TAATACAGAC TATTTGAGAC AGAGGGCCTT ATATGCATTG 
CAGGACATAG TATCCAGGCA TATTTCTGAG AACCATGAAA AAGAAGGAGA AAATATAAAG 
TCCGTGAATT CTGGTACTTG GATAGCATCC AACTCAGAAC TTACTCCCAG TGAAAGCCTT 
GCTACTACTG ATGATGAAAC TTTTGAGAAG AACTTTGAGA GAGAAACACA TAAAATAAGT 
GAGCAAAATG ATGCTGATAA TGCTAGTGTA TCTTCAACTT GTGAGCCTTT TGCAACAGAT 
GTTCTGGGCG ACACTGTGAT TCACTTAGAT CAGGCATTAG CCAGAATGAG AGACTATACG 
CATATGAAGA TGGAGGCTGA AAGTAGTCCA GACGTCAGGT GCACCTGCAG GATCATTGAG 
GATGACGATG GTGCTGCTGC CACAACCACG ATTAGTAATA ATTTAGAAGA AACTCCTGTT 
ATTGAAAATC ATAGTTCACA GCAACCCGTG AGTGAAGTTG CTACCGTCCC GTGTCCTAGA 
ATTGATACTC AGCAACTGGA CCGGCAAATT AAAGCAATTA TGAAAGAAGT CATTCCTTTT 
TTGAAGGAGC ACATGGATGA AGTATGTTCT TCTCAACTTC TAACCTCAGT AAGACGCATG 
GTTTTGGTCC TTACTCAGCA AAACAATGAG AGCAAAGAGT TTGTAAAGTT CTTTCATAAG 
CAGCTTGGAA GTATATTACA GGATTCACTC GCAAAATTTG CTGGCAGAAA ACTGAAGGAT 
TGTGGAGAAG ATCTTCTTGT AGAGATATCT GAAGTGTTAT TTAATGAATT GGCTTTCTTT 
AAGCTCATGC AAGATTTGGA TAATAATAGT GTAACTGTTA AACAGAGATG CAAAAGGAAA 
ATAGAAGCTG CTGGAGTGAT ACAGTCTTAT GCAAAAGAGG CCAAAAGGAT TCTTGAAGGA 
GATCACAGTT CACCTGCTGG AGAAATTGAT GATGAAGACA AAGACAAGGA TGAGACGGAA 
ACAGTTAAGC AGACTCAAAC GTCTGAGGTT TATGATGGTG ACGGCCCCAA AAATGTGAGA 
TCTGATGTTT CTGATCAAGA GGATGATGAA GAAAGTGAAG AATGTCCAGT GTCTATTAAT 
TTGTCCAAAG CTGAAACTCA GGCTTTGACT AATTACGGAA GTGGAGAAGA TGAGAATGAA 
GATGAAGAAA TAGAAGAATT TGAAGAGGGA CCTGTAGATG TCCAGACGTC ACTTCAGGCT 
AACACTGAAA CTACAGAAGA AAATGAACAC GATGATCAGG TTCCACAGCA TGATTTTGAA 
AAATCATCAG AAAGCAAAAA TGTCCCATCA GAACAAGAAC CCACTACTAG TAAAGGTGAC 
CAGAATAGCA CTCCTGTGAA GCCCTGTTAC CTCAGTATCG TGGAAAATGA GCAACCTTTA 
AATAGCCCTG TCCAGAAGGA CACTCTCACT ACCATCGACT CATCCAGTCA GCCTAACCCT 
TTGCCTTTAC CTTTAACTGA AATTGAAACC TTGGTGCCTA GAGTCAAAGA AGTGAAATCT 
GCTCAGGAGA CTCCTGAAAG TTCTTTGGCT GGAAGTCCTG ATACTGAATC TCCAGTGTTA 
GTGAATGACT ATGAAGCAGA ATCTGGTAAC ATAAGTCAAA AATCTGATGA AGAAGACTTT 
GTGAAAGTTG AAGATTTACC ACTTAAACTG ACAATATATT CAGAGGCAGA TCTAAGGAAG 
AAAATGGCCG AAGAAGAACA GAAAAACCAC TTATCTGGTG AAATATGTGC AATGCAGACT 
GAAGAATTAG CTGGGAATTC TCAGACACTA AAAGAACCTG AAACAGTGGG AGCCCAAAGT 
ACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025135311.1
CDS	392..6517
Translation	MATGGGPFEEGVNDQDLPNWSNESVDDRLNNMDWGSQQKKANRSSEKNKRKFGVESDKRVTNDISPESSPGVGRRRTKTSLTFPHSRYMTQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDATVSPQKREMIGSAQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIREYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLQKILARENEEEDVRTIDSAVGSGSVAESTSLNIDVRSEASDATARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSEKVAETTPGHHTVPGRQPAHSPLHQRVPIRETTGSLSGVSITSELNEELNDLIQHFHNQLRDSQPPAVPDNRRQAESLSLTREISQSRNPSVSEHLPDEKVQLFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSFVPSSASPQRSVDQRSTTTAPSAPVVLTPVVNGESNNLTPSVPYPAASLVSQNQNENEGHLNPTEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENTKEEETEESEYDSEHENSEPVTNIRNPQVAATWNEVNSNSNAQCVSNNRDGRAVNSNCEINNRSAANIRALNMPPLSDCRYNREGEQEMPVAQGHEEEEEEEEEAEDEALSGASLSSHSSSLIIETPEDAEFEHKIQKLMAAKLKLRHLQNLVAMVQDDDAANQGVTSVEDFFPAEDTKQNANNTRGNANKTQKDAGVNEKAREKFYEAKLQQQQRELKQLQEERKKLIEIQEKIQELQKACPNLQLSAASVGNCPTKTHMPAVTSSPTVNENEASTNKSGFEPHDSSVVDNEVWSDMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETASPVAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGIIRTDEEEEEEEQDASSNDNFHMYPPNSANHNSYNVKETKNRWKNNRPFSADGNYRPLAKTRQQNISMQRQENLRWVSELSYVEEKEQWQEQINQLKKQLDFSVNICQTLMQDQQTLSCLLQTLLTSPYSVMPSSVASPQVHLIMHQLNQCYTQLTWQQNNVQRLKQMLSELMRQQNQHPEKPASKEGGSGTSHPPSPSLFCPFNFPTQPVNLFNLPGFTNFSSFAPSMNFSPLFPSNFGDFSQNISTPTEQQQPLAQNPSGKTEYMAFPKPFESSSSIGAEKQRNQKQPEEEVENSRTPWFYDQEGEVEKPFLKTGFAVSVEKTTNSNRKNQLDTSRRRRQFDEVSLESFNSMLDPVDPTTVTKTFKSRKASAQASLASKDKTPKSKSKKRHSTQLKSRVKNIETGSDFSMFEALRDTIYSEVATLISQNESRPHFLIELFHELQLLNTDYLRQRALYALQDIVSRHISENHEKEGENIKSVNSGTWIASNSELTPSESLATTDDETFEKNFERETHKISEQNDADNASVSSTCEPFATDVLGDTVIHLDQALARMRDYTHMKMEAESSPDVRCTCRIIEDDDGAAATTTISNNLEETPVIENHSSQQPVSEVATVPCPRIDTQQLDRQIKAIMKEVIPFLKEHMDEVCSSQLLTSVRRMVLVLTQQNNESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSVTVKQRCKRKIEAAGVIQSYAKEAKRILEGDHSSPAGEIDDEDKDKDETETVKQTQTSEVYDGDGPKNVRSDVSDQEDDEESEECPVSINLSKAETQALTNYGSGEDENEDEEIEEFEEGPVDVQTSLQANTETTEENEHDDQVPQHDFEKSSESKNVPSEQEPTTSKGDQNSTPVKPCYLSIVENEQPLNSPVQKDTLTTIDSSSQPNPLPLPLTEIETLVPRVKEVKSAQETPESSLAGSPDTESPVLVNDYEAESGNISQKSDEEDFVKVEDLPLKLTIYSEADLRKKMAEEEQKNHLSGEICAMQTEELAGNSQTLKEPETVGAQST

Target ORF information:

RefSeq Version	XM_025279526.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis pericentriolar material 1 (PCM1), transcript variant X22, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025279526.1

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
AGCAATGAGA GTGTTGATGA CCGACTCAAC AATATGGATT GGGGCAGCCA GCAGAAGAAG 
GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA 
ACTAACGATA TTTCTCCGGA GTCATCACCG GGAGTTGGAA GACGAAGAAC AAAGACTTCA 
CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TCCCCGAGCA GGCAGAACTA 
GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCTCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCCAC TGTAAGTCCT 
CAAAAGAGAG AAATGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAGATTCGTG AGTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGACCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTCACT 
CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT CCAGAAGATT 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCGGT CTGAGGCTTC AGATGCCACG 
GCCAGAGATC CTCAGCAGGA GCCTATGGAA GAGATAGAAA ATTTGAAGAA ACAACATGAT 
TTATTAAAAA GGATGTTACA GCAGCAGGAG CAGCTGAGAG CTCTACAAGG ACGACAAGCT 
GCTCTGCTTG CCCTGCAGCA TAAAGCTGAG CAGGCTATAG CTGTGATGGA TGATTCTGAA 
AAGGTTGCGG AGACAACTCC TGGCCATCAT ACTGTACCAG GCAGACAGCC GGCTCATTCA 
CCTTTGCATC AGAGAGTACC CATAAGAGAA ACTACAGGTA GCCTATCTGG AGTCAGTATC 
ACCTCTGAAC TAAACGAAGA ATTGAATGAT TTAATTCAGC ATTTTCATAA TCAGCTTCGT 
GATTCTCAGC CTCCAGCTGT TCCAGACAAT AGAAGACAAG CAGAAAGTCT TTCATTAACT 
AGGGAGATTT CTCAGAGCAG AAATCCTTCA GTTTCAGAGC ATTTACCTGA TGAGAAAGTC 
CAACTTTTTA GCAAGATGAG AGTGTTACAA GAAAAGAAAC AAAAAATGGA CAAACTTCTT 
GGAGAACTTC ATACACTTCG AGATCAGCAT TTGAACAATT CATCATTTGT GCCTTCTTCA 
GCCTCTCCAC AAAGGAGTGT GGATCAGAGA AGTACAACCA CAGCTCCTTC TGCTCCTGTA 
GTCTTAACAC CAGTGGTCAA TGGAGAATCC AATAACCTCA CGCCATCTGT TCCTTATCCT 
GCTGCTTCTC TAGTTTCTCA GAATCAGAAT GAAAATGAAG GCCACCTAAA TCCAACTGAA 
AAACTCCAGA AGTTAAATGA AGTTCGAAAG AGGTTGAATG AACTAAGAGA ATTAGTTCAT 
TATTATGAGC AAACATCAGA TATGATGACA GATGCTGTGA ATGAAAACAC AAAAGAAGAA 
GAAACTGAAG AATCAGAATA TGATTCGGAG CATGAAAACT CGGAACCTGT TACTAACATT 
CGAAATCCAC AAGTGGCTGC CACCTGGAAC GAAGTAAATA GTAATTCTAA TGCACAGTGT 
GTTTCCAACA ATAGAGATGG GCGAGCAGTT AATTCTAACT GTGAAATTAA CAACAGGTCT 
GCTGCCAACA TAAGGGCTCT AAACATGCCT CCTCTTTCAG ATTGTCGATA CAATAGAGAA 
GGAGAACAGG AGATGCCTGT TGCACAAGGT CATGAGGAGG AGGAGGAGGA GGAGGAGGAA 
GCAGAAGATG AGGCCCTCAG TGGAGCTTCA CTGTCTAGTC ACAGCAGCAG CCTGATCATT 
GAGACTCCTG AAGATGCTGA ATTTGAACAT AAGATCCAGA AGCTTATGGC TGCAAAACTG 
AAACTTAGAC ACTTACAGAA CCTTGTAGCC ATGGTGCAGG ACGATGATGC AGCCAATCAA 
GGAGTTACTT CTGTGGAGGA TTTCTTCCCA GCAGAAGACA CCAAACAAAA TGCAAATAAC 
ACTAGAGGAA ATGCCAATAA AACACAGAAA GATGCTGGAG TAAATGAAAA GGCAAGGGAG 
AAATTTTATG AGGCTAAACT ACAGCAGCAA CAGAGAGAAC TCAAACAATT GCAGGAAGAA 
AGAAAGAAAC TGATTGAGAT TCAAGAGAAA ATTCAAGAAT TGCAAAAGGC ATGTCCTAAC 
CTACAGCTGT CAGCTGCTAG TGTGGGTAAT TGTCCCACAA AAACACATAT GCCAGCTGTT 
ACTTCATCCC CAACCGTTAA TGAAAATGAA GCCAGTACAA ACAAATCTGG TTTTGAGCCT 
CATGATTCTT CAGTGGTAGA TAATGAGGTA TGGTCAGATA TGCGAAGACA TGAAATGTTA 
AGGGAGGAGC TACGACAGAG AAGAAAGCAG CTGGAAGCTC TGATGGCTGA ACATCAGAGG 
AGGCAAGGTC TGGCTGAAAC TGCATCTCCA GTGGCTGTTT CACTGAGAAG TGATGGATCT 
GAGAACCTGT GTACACCTCA GCAAAGCAGA ACAGAAAAAA CAATGGCAAC TTGGGGAGGT 
TCTACCCAGT GTGCCCTAGA TGAAGAAGGA GATGAAGATG GTTACCTTTC TGAAGGAATT 
ATTCGGACAG ACGAAGAGGA GGAAGAGGAG GAGCAAGATG CCAGTTCCAA TGATAACTTT 
CATATGTATC CTCCAAACAG TGCAAATCAT AATTCTTACA ATGTAAAGGA AACTAAAAAT 
AGGTGGAAGA ACAATCGCCC TTTTTCAGCA GATGGAAATT ATCGTCCTTT AGCCAAGACA 
AGACAGCAGA ATATCAGCAT GCAGCGTCAG GAAAACCTTC GCTGGGTGTC CGAACTCTCT 
TATGTAGAAG AGAAAGAACA ATGGCAAGAA CAAATTAATC AGCTAAAGAA ACAACTTGAT 
TTCAGTGTCA ATATTTGTCA GACTTTAATG CAAGACCAGC AGACTCTGTC TTGTCTGCTA 
CAAACTCTTC TCACGAGTCC TTATAGTGTT ATGCCCAGCA GTGTTGCATC CCCGCAAGTG 
CACCTTATAA TGCACCAGTT GAATCAGTGC TATACCCAGC TAACGTGGCA GCAGAACAAT 
GTCCAGAGGT TGAAACAAAT GCTCAGTGAA CTCATGCGCC AACAAAATCA GCATCCAGAA 
AAACCTGCAA GCAAGGAAGG AGGCAGTGGT ACATCACACC CTCCTTCTCC CAGTTTATTT 
TGTCCTTTCA ACTTTCCAAC TCAGCCTGTA AATCTGTTTA ACCTACCGGG GTTTACTAAT 
TTTTCGTCAT TTGCACCAAG TATGAATTTC AGCCCTTTAT TTCCTTCTAA TTTTGGAGAT 
TTTTCTCAAA ATATTTCTAC ACCCACTGAA CAGCAGCAAC CGTTAGCCCA GAATCCTTCA 
GGGAAAACGG AATACATGGC TTTTCCAAAA CCTTTTGAAA GTAGTTCTTC TATTGGAGCG 
GAAAAACAAA GGAATCAAAA ACAGCCTGAA GAGGAGGTGG AGAACAGTAG GACACCATGG 
TTTTATGACC AAGAAGGTGA AGTAGAAAAG CCATTTCTCA AGACTGGATT TGCAGTGTCT 
GTAGAGAAAA CAACAAATAG TAATCGCAAA AATCAACTAG ATACCAGTAG GAGAAGACGC 
CAGTTTGATG AAGTGTCCTT AGAAAGCTTT AACAGTATGC TTGACCCAGT AGATCCAACA 
ACAGTAACTA AAACATTCAA GTCAAGAAAA GCATCTGCAC AGGCCAGCCT GGCATCTAAA 
GATAAAACTC CCAAATCAAA GAGTAAGAAG AGGCATTCTA CTCAGCTGAA AAGCAGAGTG 
AAAAATATCG AAACCGGGAG TGATTTTTCC ATGTTCGAAG CTTTGCGGGA TACCATTTAT 
TCTGAAGTAG CTACGTTGAT TTCTCAAAAT GAATCTCGTC CACATTTTCT TATTGAACTC 
TTCCATGAGC TTCAGCTACT TAATACAGAC TATTTGAGAC AGAGGGCCTT ATATGCATTG 
CAGGACATAG TATCCAGGCA TATTTCTGAG AACCATGAAA AAGAAGGAGA AAATATAAAG 
TCCGTGAATT CTGGTACTTG GATAGCATCC AACTCAGAAC TTACTCCCAG TGAAAGCCTT 
GCTACTACTG ATGATGAAAC TTTTGAGAAG AACTTTGAGA GAGAAACACA TAAAATAAGT 
GAGCAAAATG ATGCTGATAA TGCTAGTGTA TCTTCAACTT GTGAGCCTTT TGCAACAGAT 
GTTCTGGGCG ACACTGTGAT TCACTTAGAT CAGGCATTAG CCAGAATGAG AGACTATACG 
CATATGAAGA TGGAGGCTGA AAGTAGTCCA GACGTCAGGT GCACCTGCAG GATCATTGAG 
GATGACGATG GTGCTGCTGC CACAACCACG ATTAGTAATA ATTTAGAAGA AACTCCTGTT 
ATTGAAAATC ATAGTTCACA GCAACCCGTG AGTGAAGTTG CTACCGTCCC GTGTCCTAGA 
ATTGATACTC AGCAACTGGA CCGGCAAATT AAAGCAATTA TGAAAGAAGT CATTCCTTTT 
TTGAAGGAGC ACATGGATGA AGTATGTTCT TCTCAACTTC TAACCTCAGT AAGACGCATG 
GTTTTGGTCC TTACTCAGCA AAACAATGAG AGCAAAGAGT TTGTAAAGTT CTTTCATAAG 
CAGCTTGGAA GTATATTACA GGATTCACTC GCAAAATTTG CTGGCAGAAA ACTGAAGGAT 
TGTGGAGAAG ATCTTCTTGT AGAGATATCT GAAGTGTTAT TTAATGAATT GGCTTTCTTT 
AAGCTCATGC AAGATTTGGA TAATAATAGT GTAACTGTTA AACAGAGATG CAAAAGGAAA 
ATAGAAGCTG CTGGAGTGAT ACAGTCTTAT GCAAAAGAGG CCAAAAGGAT TCTTGAAGGA 
GATCACAGTT CACCTGCTGG AGAAATTGAT GATGAAGACA AAGACAAGGA TGAGACGGAA 
ACAGTTAAGC AGACTCAAAC GTCTGAGGTT TATGATGGTG ACGGCCCCAA AAATGTGAGA 
TCTGATGTTT CTGATCAAGA GGATGATGAA GAAAGTGAAG AATGTCCAGT GTCTATTAAT 
TTGTCCAAAG CTGAAACTCA GGCTTTGACT AATTACGGAA GTGGAGAAGA TGAGAATGAA 
GATGAAGAAA TAGAAGAATT TGAAGAGGGA CCTGTAGATG TCCAGACGTC ACTTCAGGCT 
AACACTGAAA CTACAGAAGA AAATGAACAC GATGATCAGG TTCCACAGCA TGATTTTGAA 
AAATCATCAG AAAGCAAAAA TGTCCCATCA GAACAAGAAC CCACTACTAG TAAAGGTGAC 
CAGAATAGCA CTCCTGTGAA GCCCTGTTAC CTCAGTATCG TGGAAAATGA GCAACCTTTA 
AATAGCCCTG TCCAGAAGGA CACTCTCACT ACCATCGACT CATCCAGTCA GCCTAACCCT 
TTGCCTTTAC CTTTAACTGA AATTGAAACC TTGGTGCCTA GAGTCAAAGA AGTGAAATCT 
GCTCAGGAGA CTCCTGAAAG TTCTTTGGCT GGAAGTCCTG ATACTGAATC TCCAGTGTTA 
GTGAATGACT ATGAAGCAGA ATCTGGTAAC ATAAGTCAAA AATCTGATGA AGAAGACTTT 
GTGAAAGTTG AAGATTTACC ACTTAAACTG ACAATATATT CAGAGGCAGA TCTAAGGAAG 
AAAATGGCCG AAGAAGAACA GAAAAACCAC TTATCTGGTG AAATATGTGC AATGCAGACT 
GAAGAATTAG CTGGGAATTC TCAGACACTA AAAGAACCTG AAACAGTGGG AGCCCAAAGT 
ACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( XM_025279531.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025135316.1 Bubalus bubalis pericentriolar material 1 (PCM1), transcript variant X23, mRNA.

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CloneID	OBu42311
Clone ID Related Accession (Same CDS sequence)	XM_025279531.1
Accession Version	XM_025279531.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6177bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	pericentriolar material 1 protein isoform X22
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037545.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG GAGGAGGTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
AGCAATGAGA GTGTTGATGA CCGACTCAAC AATATGGATT GGGGCAGCCA GCAGAAGAAG 
GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA 
ACTAACGATA TTTCTCCGGA GTCATCACCG GGAGTTGGAA GACGAAGAAC AAAGACTTCA 
CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TCCCCGAGCA GGCAGAACTA 
GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCTCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCCAC TGTAAGTCCT 
CAAAAGAGAG AAATGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAGATTCGTG AGTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGACCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTCACT 
CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT CCAGAAGATT 
CTTGCCAGAG ATCCTCAGCA GGAGCCTATG GAAGAGATAG AAAATTTGAA GAAACAACAT 
GATTTATTAA AAAGGATGTT ACAGCAGCAG GAGCAGCTGA GAGCTCTACA AGGACGACAA 
GCTGCTCTGC TTGCCCTGCA GCATAAAGCT GAGCAGGCTA TAGCTGTGAT GGATGATTCT 
GAAAAGGTTG CGGAGACAAC TCCTGGCCAT CATACTGTAC CAGGCAGACA GCCGGCTCAT 
TCACCTTTGC ATCAGAGAGT ACCCATAAGA GAAACTACAG GTAGCCTATC TGGAGTCAGT 
ATCACCTCTG AACTAAACGA AGAATTGAAT GATTTAATTC AGCATTTTCA TAATCAGCTT 
CGTGATTCTC AGCCTCCAGC TGTTCCAGAC AATAGAAGAC AAGCAGAAAG TCTTTCATTA 
ACTAGGGAGA TTTCTCAGAG CAGAAATCCT TCAGTTTCAG AGCATTTACC TGATGAGAAA 
GTCCAACTTT TTAGCAAGAT GAGAGTGTTA CAAGAAAAGA AACAAAAAAT GGACAAACTT 
CTTGGAGAAC TTCATACACT TCGAGATCAG CATTTGAACA ATTCATCATT TGTGCCTTCT 
TCAGCCTCTC CACAAAGGAG TGTGGATCAG AGAAGTACAA CCACAGCTCC TTCTGCTCCT 
GTAGTCTTAA CACCAGTGGT CAATGGAGAA TCCAATAACC TCACGCCATC TGTTCCTTAT 
CCTGCTGCTT CTCTAGTTTC TCAGAATCAG AATGAAAATG AAGGCCACCT AAATCCAACT 
GAAAAACTCC AGAAGTTAAA TGAAGTTCGA AAGAGGTTGA ATGAACTAAG AGAATTAGTT 
CATTATTATG AGCAAACATC AGATATGATG ACAGATGCTG TGAATGAAAA CACAAAAGAA 
GAAGAAACTG AAGAATCAGA ATATGATTCG GAGCATGAAA ACTCGGAACC TGTTACTAAC 
ATTCGAAATC CACAAGTGGC TGCCACCTGG AACGAAGTAA ATAGTAATTC TAATGCACAG 
TGTGTTTCCA ACAATAGAGA TGGGCGAGCA GTTAATTCTA ACTGTGAAAT TAACAACAGG 
TCTGCTGCCA ACATAAGGGC TCTAAACATG CCTCCTCTTT CAGCAGATTG TCGATACAAT 
AGAGAAGGAG AACAGGAGAT GCCTGTTGCA CAAGGTCATG AGGAGGAGGA GGAGGAGGAG 
GAGGAAGCAG AAGATGAGGC CCTCAGTGGA GCTTCACTGT CTAGTCACAG CAGCAGCCTG 
ATCATTGAGA CTCCTGAAGA TGCTGAATTT GAACATAAGA TCCAGAAGCT TATGGCTGCA 
AAACTGAAAC TTAGACACTT ACAGAACCTT GTAGCCATGG TGCAGGACGA TGATGCAGCC 
AATCAAGGAG TTACTTCTGT GGAGGATTTC TTCCCAGCAG AAGACACCAA ACAAAATGCA 
AATAACACTA GAGGAAATGC CAATAAAACA CAGAAAGATG CTGGAGTAAA TGAAAAGGCA 
AGGGAGAAAT TTTATGAGGC TAAACTACAG CAGCAACAGA GAGAACTCAA ACAATTGCAG 
GAAGAAAGAA AGAAACTGAT TGAGATTCAA GAGAAAATTC AAGAATTGCA AAAGGCATGT 
CCTAACCTAC AGCTGTCAGC TGCTAGTGTG GGTAATTGTC CCACAAAAAC ACATATGCCA 
GCTGTTACTT CATCCCCAAC CGTTAATGAA AATGAAGCCA GTACAAACAA ATCTGGTTTT 
GAGCCTCATG ATTCTTCAGT GGTAGATAAT GAGGTATGGT CAGATATGCG AAGACATGAA 
ATGTTAAGGG AGGAGCTACG ACAGAGAAGA AAGCAGCTGG AAGCTCTGAT GGCTGAACAT 
CAGAGGAGGC AAGGTCTGGC TGAAACTGCA TCTCCAGTGG CTGTTTCACT GAGAAGTGAT 
GGATCTGAGA ACCTGTGTAC ACCTCAGCAA AGCAGAACAG AAAAAACAAT GGCAACTTGG 
GGAGGTTCTA CCCAGTGTGC CCTAGATGAA GAAGGAGATG AAGATGGTTA CCTTTCTGAA 
GGAATTATTC GGACAGACGA AGAGGAGGAA GAGGAGGAGC AAGATGCCAG TTCCAATGAT 
AACTTTCATA TGTATCCTCC AAACAGTGCA AATCATAATT CTTACAATGT AAAGGAAACT 
AAAAATAGGT GGAAGAACAA TCGCCCTTTT TCAGCAGATG GAAATTATCG TCCTTTAGCC 
AAGACAAGAC AGCAGAATAT CAGCATGCAG CGTCAGGAAA ACCTTCGCTG GGTGTCCGAA 
CTCTCTTATG TAGAAGAGAA AGAACAATGG CAAGAACAAA TTAATCAGCT AAAGAAACAA 
CTTGATTTCA GTGTCAATAT TTGTCAGACT TTAATGCAAG ACCAGCAGAC TCTGTCTTGT 
CTGCTACAAA CTCTTCTCAC GAGTCCTTAT AGTGTTATGC CCAGCAGTGT TGCATCCCCG 
CAAGTGCACC TTATAATGCA CCAGTTGAAT CAGTGCTATA CCCAGCTAAC GTGGCAGCAG 
AACAATGTCC AGAGGTTGAA ACAAATGCTC AGTGAACTCA TGCGCCAACA AAATCAGCAT 
CCAGAAAAAC CTGCAAGCAA GGAAGGAGGC AGTGGTACAT CACACCCTCC TTCTCCCAGT 
TTATTTTGTC CTTTCAACTT TCCAACTCAG CCTGTAAATC TGTTTAACCT ACCGGGGTTT 
ACTAATTTTT CGTCATTTGC ACCAAGTATG AATTTCAGCC CTTTATTTCC TTCTAATTTT 
GGAGATTTTT CTCAAAATAT TTCTACACCC ACTGAACAGC AGCAACCGTT AGCCCAGAAT 
CCTTCAGGGA AAACGGAATA CATGGCTTTT CCAAAACCTT TTGAAAGTAG TTCTTCTATT 
GGAGCGGAAA AACAAAGGAA TCAAAAACAG CCTGAAGAGG AGGTGGAGAA CAGTAGGACA 
CCATGGTTTT ATGACCAAGA AGGTGAAGTA GAAAAGCCAT TTCTCAAGAC TGGATTTGCA 
GTGTCTGTAG AGAAAACAAC AAATAGTAAT CGCAAAAATC AACTAGATAC CAGTAGGAGA 
AGACGCCAGT TTGATGAAGT GTCCTTAGAA AGCTTTAACA GTATGCTTGA CCCAGTAGAT 
CCAACAACAG TAACTAAAAC ATTCAAGTCA AGAAAAGCAT CTGCACAGGC CAGCCTGGCA 
TCTAAAGATA AAACTCCCAA ATCAAAGAGT AAGAAGAGGC ATTCTACTCA GCTGAAAAGC 
AGAGTGAAAA ATATCGGGTA TGAAAGTGCC AGTGTGTCTA GCACATGTGA ACCTTGCAAA 
AGTAGAAACA GACATTCAGC CCAAACTGAA GAGCCTGTTC AAGCAAAAGT ACTCAGCAGA 
AAGAATCATG AGCAGCTGGA AAAAGTCATA AGATGTAGTA GGTCTACAGA AATATCTTCA 
GAAACCGGGA GTGATTTTTC CATGTTCGAA GCTTTGCGGG ATACCATTTA TTCTGAAGTA 
GCTACGTTGA TTTCTCAAAA TGAATCTCGT CCACATTTTC TTATTGAACT CTTCCATGAG 
CTTCAGCTAC TTAATACAGA CTATTTGAGA CAGAGGGCCT TATATGCATT GCAGGACATA 
GTATCCAGGC ATATTTCTGA GAACCATGAA AAAGAAGGAG AAAATATAAA GTCCGTGAAT 
TCTGGTACTT GGATAGCATC CAACTCAGAA CTTACTCCCA GTGAAAGCCT TGCTACTACT 
GATGATGAAA CTTTTGAGAA GAACTTTGAG AGAGAAACAC ATAAAATAAG TGAGCAAAAT 
GATGCTGATA ATGCTAGTGT ATCTTCAACT TGTGAGCCTT TTGCAACAGA TGTTCTGGGC 
GACACTGTGA TTCACTTAGA TCAGGCATTA GCCAGAATGA GAGACTATAC GCATATGAAG 
ATGGAGGCTG AAAGTAGTCC AGACGTCAGG TGCACCTGCA GGATCATTGA GGATGACGAT 
GGTGCTGCTG CCACAACCAC GATTAGTAAT AATTTAGAAG AAACTCCTGT TATTGAAAAT 
CATAGTTCAC AGCAACCCGT GAGTGAAGTT GCTACCGTCC CGTGTCCTAG AATTGATACT 
CAGCAACTGG ACCGGCAAAT TAAAGCAATT ATGAAAGAAG TCATTCCTTT TTTGAAGGAG 
CACATGGATG AAGTATGTTC TTCTCAACTT CTAACCTCAG TAAGACGCAT GGTTTTGGTC 
CTTACTCAGC AAAACAATGA GAGCAAAGAG TTTGTAAAGT TCTTTCATAA GCAGCTTGGA 
AGTATATTAC AGGATTCACT CGCAAAATTT GCTGGCAGAA AACTGAAGGA TTGTGGAGAA 
GATCTTCTTG TAGAGATATC TGAAGTGTTA TTTAATGAAT TGGCTTTCTT TAAGCTCATG 
CAAGATTTGG ATAATAATAG TGTAACTGTT AAACAGAGAT GCAAAAGGAA AATAGAAGCT 
GCTGGAGTGA TACAGTCTTA TGCAAAAGAG GCCAAAAGGA TTCTTGAAGG AGATCACAGT 
TCACCTGCTG GAGAAATTGA TGATGAAGAC AAAGACAAGG ATGAGACGGA AACAGTTAAG 
CAGACTCAAA CGTCTGAGGT TTATGATGGT GACGGCCCCA AAAATGTGAG ATCTGATGTT 
TCTGATCAAG AGGATGATGA AGAAAGTGAA GAATGTCCAG TGTCTATTAA TTTGTCCAAA 
GCTGAAACTC AGGCTTTGAC TAATTACGGA AGTGGAGAAG ATGAGAATGA AGATGAAGAA 
ATAGAAGAAT TTGAAGAGGG ACCTGTAGAT GTCCAGACGT CACTTCAGGC TAACACTGAA 
ACTACAGAAG AAAATGAACA CGATGATCAG GTTCCACAGC ATGATTTTGA AAAATCATCA 
GAAAGCAAAA ATGTCCCATC AGAACAAGAA CCCACTACTA GTAAAGGTGA CCAGAATAGC 
ACTCCTGTGA AGCCCTGTTA CCTCAGTATC GTGGAAAATG AGCAACCTTT AAATAGCCCT 
GTCCAGAAGG ACACTCTCAC TACCATCGAC TCATCCAGTC AGCCTAACCC TTTGCCTTTA 
CCTTTAACTG AAATTGAAAC CTTGGTGCCT AGAGTCAAAG AAGTGAAATC TGCTCAGGAG 
ACTCCTGAAA GTTCTTTGGC TGGAAGTCCT GATACTGAAT CTCCAGTGTT AGTGAATGAC 
TATGAAGCAG AATCTGGTAA CATAAGTCAA AAATCTGATG AAGAAGACTT TGTGAAAGTT 
GAAGATTTAC CACTTAAACT GACAATATAT TCAGAGGCAG ATCTAAGGAA GAAAATGGCC 
GAAGAAGAAC AGAAAAACCA CTTATCTGGT GAAATATGTG CAATGCAGAC TGAAGAATTA 
GCTGGGAATT CTCAGACACT AAAAGAACCT GAAACAGTGG GAGCCCAAAG TACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025135316.1
CDS	392..6568
Translation	MATGGGPFEEGVNDQDLPNWSNESVDDRLNNMDWGSQQKKANRSSEKNKRKFGVESDKRVTNDISPESSPGVGRRRTKTSLTFPHSRYMTQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDATVSPQKREMIGSAQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIREYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLQKILARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSEKVAETTPGHHTVPGRQPAHSPLHQRVPIRETTGSLSGVSITSELNEELNDLIQHFHNQLRDSQPPAVPDNRRQAESLSLTREISQSRNPSVSEHLPDEKVQLFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSFVPSSASPQRSVDQRSTTTAPSAPVVLTPVVNGESNNLTPSVPYPAASLVSQNQNENEGHLNPTEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENTKEEETEESEYDSEHENSEPVTNIRNPQVAATWNEVNSNSNAQCVSNNRDGRAVNSNCEINNRSAANIRALNMPPLSADCRYNREGEQEMPVAQGHEEEEEEEEEAEDEALSGASLSSHSSSLIIETPEDAEFEHKIQKLMAAKLKLRHLQNLVAMVQDDDAANQGVTSVEDFFPAEDTKQNANNTRGNANKTQKDAGVNEKAREKFYEAKLQQQQRELKQLQEERKKLIEIQEKIQELQKACPNLQLSAASVGNCPTKTHMPAVTSSPTVNENEASTNKSGFEPHDSSVVDNEVWSDMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETASPVAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGIIRTDEEEEEEEQDASSNDNFHMYPPNSANHNSYNVKETKNRWKNNRPFSADGNYRPLAKTRQQNISMQRQENLRWVSELSYVEEKEQWQEQINQLKKQLDFSVNICQTLMQDQQTLSCLLQTLLTSPYSVMPSSVASPQVHLIMHQLNQCYTQLTWQQNNVQRLKQMLSELMRQQNQHPEKPASKEGGSGTSHPPSPSLFCPFNFPTQPVNLFNLPGFTNFSSFAPSMNFSPLFPSNFGDFSQNISTPTEQQQPLAQNPSGKTEYMAFPKPFESSSSIGAEKQRNQKQPEEEVENSRTPWFYDQEGEVEKPFLKTGFAVSVEKTTNSNRKNQLDTSRRRRQFDEVSLESFNSMLDPVDPTTVTKTFKSRKASAQASLASKDKTPKSKSKKRHSTQLKSRVKNIGYESASVSSTCEPCKSRNRHSAQTEEPVQAKVLSRKNHEQLEKVIRCSRSTEISSETGSDFSMFEALRDTIYSEVATLISQNESRPHFLIELFHELQLLNTDYLRQRALYALQDIVSRHISENHEKEGENIKSVNSGTWIASNSELTPSESLATTDDETFEKNFERETHKISEQNDADNASVSSTCEPFATDVLGDTVIHLDQALARMRDYTHMKMEAESSPDVRCTCRIIEDDDGAAATTTISNNLEETPVIENHSSQQPVSEVATVPCPRIDTQQLDRQIKAIMKEVIPFLKEHMDEVCSSQLLTSVRRMVLVLTQQNNESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSVTVKQRCKRKIEAAGVIQSYAKEAKRILEGDHSSPAGEIDDEDKDKDETETVKQTQTSEVYDGDGPKNVRSDVSDQEDDEESEECPVSINLSKAETQALTNYGSGEDENEDEEIEEFEEGPVDVQTSLQANTETTEENEHDDQVPQHDFEKSSESKNVPSEQEPTTSKGDQNSTPVKPCYLSIVENEQPLNSPVQKDTLTTIDSSSQPNPLPLPLTEIETLVPRVKEVKSAQETPESSLAGSPDTESPVLVNDYEAESGNISQKSDEEDFVKVEDLPLKLTIYSEADLRKKMAEEEQKNHLSGEICAMQTEELAGNSQTLKEPETVGAQST

Target ORF information:

RefSeq Version	XM_025279531.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis pericentriolar material 1 (PCM1), transcript variant X23, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025279531.1

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
AGCAATGAGA GTGTTGATGA CCGACTCAAC AATATGGATT GGGGCAGCCA GCAGAAGAAG 
GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA 
ACTAACGATA TTTCTCCGGA GTCATCACCG GGAGTTGGAA GACGAAGAAC AAAGACTTCA 
CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TCCCCGAGCA GGCAGAACTA 
GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCTCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCCAC TGTAAGTCCT 
CAAAAGAGAG AAATGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAGATTCGTG AGTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGACCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTCACT 
CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT CCAGAAGATT 
CTTGCCAGAG ATCCTCAGCA GGAGCCTATG GAAGAGATAG AAAATTTGAA GAAACAACAT 
GATTTATTAA AAAGGATGTT ACAGCAGCAG GAGCAGCTGA GAGCTCTACA AGGACGACAA 
GCTGCTCTGC TTGCCCTGCA GCATAAAGCT GAGCAGGCTA TAGCTGTGAT GGATGATTCT 
GAAAAGGTTG CGGAGACAAC TCCTGGCCAT CATACTGTAC CAGGCAGACA GCCGGCTCAT 
TCACCTTTGC ATCAGAGAGT ACCCATAAGA GAAACTACAG GTAGCCTATC TGGAGTCAGT 
ATCACCTCTG AACTAAACGA AGAATTGAAT GATTTAATTC AGCATTTTCA TAATCAGCTT 
CGTGATTCTC AGCCTCCAGC TGTTCCAGAC AATAGAAGAC AAGCAGAAAG TCTTTCATTA 
ACTAGGGAGA TTTCTCAGAG CAGAAATCCT TCAGTTTCAG AGCATTTACC TGATGAGAAA 
GTCCAACTTT TTAGCAAGAT GAGAGTGTTA CAAGAAAAGA AACAAAAAAT GGACAAACTT 
CTTGGAGAAC TTCATACACT TCGAGATCAG CATTTGAACA ATTCATCATT TGTGCCTTCT 
TCAGCCTCTC CACAAAGGAG TGTGGATCAG AGAAGTACAA CCACAGCTCC TTCTGCTCCT 
GTAGTCTTAA CACCAGTGGT CAATGGAGAA TCCAATAACC TCACGCCATC TGTTCCTTAT 
CCTGCTGCTT CTCTAGTTTC TCAGAATCAG AATGAAAATG AAGGCCACCT AAATCCAACT 
GAAAAACTCC AGAAGTTAAA TGAAGTTCGA AAGAGGTTGA ATGAACTAAG AGAATTAGTT 
CATTATTATG AGCAAACATC AGATATGATG ACAGATGCTG TGAATGAAAA CACAAAAGAA 
GAAGAAACTG AAGAATCAGA ATATGATTCG GAGCATGAAA ACTCGGAACC TGTTACTAAC 
ATTCGAAATC CACAAGTGGC TGCCACCTGG AACGAAGTAA ATAGTAATTC TAATGCACAG 
TGTGTTTCCA ACAATAGAGA TGGGCGAGCA GTTAATTCTA ACTGTGAAAT TAACAACAGG 
TCTGCTGCCA ACATAAGGGC TCTAAACATG CCTCCTCTTT CAGCAGATTG TCGATACAAT 
AGAGAAGGAG AACAGGAGAT GCCTGTTGCA CAAGGTCATG AGGAGGAGGA GGAGGAGGAG 
GAGGAAGCAG AAGATGAGGC CCTCAGTGGA GCTTCACTGT CTAGTCACAG CAGCAGCCTG 
ATCATTGAGA CTCCTGAAGA TGCTGAATTT GAACATAAGA TCCAGAAGCT TATGGCTGCA 
AAACTGAAAC TTAGACACTT ACAGAACCTT GTAGCCATGG TGCAGGACGA TGATGCAGCC 
AATCAAGGAG TTACTTCTGT GGAGGATTTC TTCCCAGCAG AAGACACCAA ACAAAATGCA 
AATAACACTA GAGGAAATGC CAATAAAACA CAGAAAGATG CTGGAGTAAA TGAAAAGGCA 
AGGGAGAAAT TTTATGAGGC TAAACTACAG CAGCAACAGA GAGAACTCAA ACAATTGCAG 
GAAGAAAGAA AGAAACTGAT TGAGATTCAA GAGAAAATTC AAGAATTGCA AAAGGCATGT 
CCTAACCTAC AGCTGTCAGC TGCTAGTGTG GGTAATTGTC CCACAAAAAC ACATATGCCA 
GCTGTTACTT CATCCCCAAC CGTTAATGAA AATGAAGCCA GTACAAACAA ATCTGGTTTT 
GAGCCTCATG ATTCTTCAGT GGTAGATAAT GAGGTATGGT CAGATATGCG AAGACATGAA 
ATGTTAAGGG AGGAGCTACG ACAGAGAAGA AAGCAGCTGG AAGCTCTGAT GGCTGAACAT 
CAGAGGAGGC AAGGTCTGGC TGAAACTGCA TCTCCAGTGG CTGTTTCACT GAGAAGTGAT 
GGATCTGAGA ACCTGTGTAC ACCTCAGCAA AGCAGAACAG AAAAAACAAT GGCAACTTGG 
GGAGGTTCTA CCCAGTGTGC CCTAGATGAA GAAGGAGATG AAGATGGTTA CCTTTCTGAA 
GGAATTATTC GGACAGACGA AGAGGAGGAA GAGGAGGAGC AAGATGCCAG TTCCAATGAT 
AACTTTCATA TGTATCCTCC AAACAGTGCA AATCATAATT CTTACAATGT AAAGGAAACT 
AAAAATAGGT GGAAGAACAA TCGCCCTTTT TCAGCAGATG GAAATTATCG TCCTTTAGCC 
AAGACAAGAC AGCAGAATAT CAGCATGCAG CGTCAGGAAA ACCTTCGCTG GGTGTCCGAA 
CTCTCTTATG TAGAAGAGAA AGAACAATGG CAAGAACAAA TTAATCAGCT AAAGAAACAA 
CTTGATTTCA GTGTCAATAT TTGTCAGACT TTAATGCAAG ACCAGCAGAC TCTGTCTTGT 
CTGCTACAAA CTCTTCTCAC GAGTCCTTAT AGTGTTATGC CCAGCAGTGT TGCATCCCCG 
CAAGTGCACC TTATAATGCA CCAGTTGAAT CAGTGCTATA CCCAGCTAAC GTGGCAGCAG 
AACAATGTCC AGAGGTTGAA ACAAATGCTC AGTGAACTCA TGCGCCAACA AAATCAGCAT 
CCAGAAAAAC CTGCAAGCAA GGAAGGAGGC AGTGGTACAT CACACCCTCC TTCTCCCAGT 
TTATTTTGTC CTTTCAACTT TCCAACTCAG CCTGTAAATC TGTTTAACCT ACCGGGGTTT 
ACTAATTTTT CGTCATTTGC ACCAAGTATG AATTTCAGCC CTTTATTTCC TTCTAATTTT 
GGAGATTTTT CTCAAAATAT TTCTACACCC ACTGAACAGC AGCAACCGTT AGCCCAGAAT 
CCTTCAGGGA AAACGGAATA CATGGCTTTT CCAAAACCTT TTGAAAGTAG TTCTTCTATT 
GGAGCGGAAA AACAAAGGAA TCAAAAACAG CCTGAAGAGG AGGTGGAGAA CAGTAGGACA 
CCATGGTTTT ATGACCAAGA AGGTGAAGTA GAAAAGCCAT TTCTCAAGAC TGGATTTGCA 
GTGTCTGTAG AGAAAACAAC AAATAGTAAT CGCAAAAATC AACTAGATAC CAGTAGGAGA 
AGACGCCAGT TTGATGAAGT GTCCTTAGAA AGCTTTAACA GTATGCTTGA CCCAGTAGAT 
CCAACAACAG TAACTAAAAC ATTCAAGTCA AGAAAAGCAT CTGCACAGGC CAGCCTGGCA 
TCTAAAGATA AAACTCCCAA ATCAAAGAGT AAGAAGAGGC ATTCTACTCA GCTGAAAAGC 
AGAGTGAAAA ATATCGGGTA TGAAAGTGCC AGTGTGTCTA GCACATGTGA ACCTTGCAAA 
AGTAGAAACA GACATTCAGC CCAAACTGAA GAGCCTGTTC AAGCAAAAGT ACTCAGCAGA 
AAGAATCATG AGCAGCTGGA AAAAGTCATA AGATGTAGTA GGTCTACAGA AATATCTTCA 
GAAACCGGGA GTGATTTTTC CATGTTCGAA GCTTTGCGGG ATACCATTTA TTCTGAAGTA 
GCTACGTTGA TTTCTCAAAA TGAATCTCGT CCACATTTTC TTATTGAACT CTTCCATGAG 
CTTCAGCTAC TTAATACAGA CTATTTGAGA CAGAGGGCCT TATATGCATT GCAGGACATA 
GTATCCAGGC ATATTTCTGA GAACCATGAA AAAGAAGGAG AAAATATAAA GTCCGTGAAT 
TCTGGTACTT GGATAGCATC CAACTCAGAA CTTACTCCCA GTGAAAGCCT TGCTACTACT 
GATGATGAAA CTTTTGAGAA GAACTTTGAG AGAGAAACAC ATAAAATAAG TGAGCAAAAT 
GATGCTGATA ATGCTAGTGT ATCTTCAACT TGTGAGCCTT TTGCAACAGA TGTTCTGGGC 
GACACTGTGA TTCACTTAGA TCAGGCATTA GCCAGAATGA GAGACTATAC GCATATGAAG 
ATGGAGGCTG AAAGTAGTCC AGACGTCAGG TGCACCTGCA GGATCATTGA GGATGACGAT 
GGTGCTGCTG CCACAACCAC GATTAGTAAT AATTTAGAAG AAACTCCTGT TATTGAAAAT 
CATAGTTCAC AGCAACCCGT GAGTGAAGTT GCTACCGTCC CGTGTCCTAG AATTGATACT 
CAGCAACTGG ACCGGCAAAT TAAAGCAATT ATGAAAGAAG TCATTCCTTT TTTGAAGGAG 
CACATGGATG AAGTATGTTC TTCTCAACTT CTAACCTCAG TAAGACGCAT GGTTTTGGTC 
CTTACTCAGC AAAACAATGA GAGCAAAGAG TTTGTAAAGT TCTTTCATAA GCAGCTTGGA 
AGTATATTAC AGGATTCACT CGCAAAATTT GCTGGCAGAA AACTGAAGGA TTGTGGAGAA 
GATCTTCTTG TAGAGATATC TGAAGTGTTA TTTAATGAAT TGGCTTTCTT TAAGCTCATG 
CAAGATTTGG ATAATAATAG TGTAACTGTT AAACAGAGAT GCAAAAGGAA AATAGAAGCT 
GCTGGAGTGA TACAGTCTTA TGCAAAAGAG GCCAAAAGGA TTCTTGAAGG AGATCACAGT 
TCACCTGCTG GAGAAATTGA TGATGAAGAC AAAGACAAGG ATGAGACGGA AACAGTTAAG 
CAGACTCAAA CGTCTGAGGT TTATGATGGT GACGGCCCCA AAAATGTGAG ATCTGATGTT 
TCTGATCAAG AGGATGATGA AGAAAGTGAA GAATGTCCAG TGTCTATTAA TTTGTCCAAA 
GCTGAAACTC AGGCTTTGAC TAATTACGGA AGTGGAGAAG ATGAGAATGA AGATGAAGAA 
ATAGAAGAAT TTGAAGAGGG ACCTGTAGAT GTCCAGACGT CACTTCAGGC TAACACTGAA 
ACTACAGAAG AAAATGAACA CGATGATCAG GTTCCACAGC ATGATTTTGA AAAATCATCA 
GAAAGCAAAA ATGTCCCATC AGAACAAGAA CCCACTACTA GTAAAGGTGA CCAGAATAGC 
ACTCCTGTGA AGCCCTGTTA CCTCAGTATC GTGGAAAATG AGCAACCTTT AAATAGCCCT 
GTCCAGAAGG ACACTCTCAC TACCATCGAC TCATCCAGTC AGCCTAACCC TTTGCCTTTA 
CCTTTAACTG AAATTGAAAC CTTGGTGCCT AGAGTCAAAG AAGTGAAATC TGCTCAGGAG 
ACTCCTGAAA GTTCTTTGGC TGGAAGTCCT GATACTGAAT CTCCAGTGTT AGTGAATGAC 
TATGAAGCAG AATCTGGTAA CATAAGTCAA AAATCTGATG AAGAAGACTT TGTGAAAGTT 
GAAGATTTAC CACTTAAACT GACAATATAT TCAGAGGCAG ATCTAAGGAA GAAAATGGCC 
GAAGAAGAAC AGAAAAACCA CTTATCTGGT GAAATATGTG CAATGCAGAC TGAAGAATTA 
GCTGGGAATT CTCAGACACT AAAAGAACCT GAAACAGTGG GAGCCCAAAG TACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( XM_025279539.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025135324.1 Bubalus bubalis pericentriolar material 1 (PCM1), transcript variant X24, mRNA.

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CloneID	OBu42312
Clone ID Related Accession (Same CDS sequence)	XM_025279539.1
Accession Version	XM_025279539.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6012bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	pericentriolar material 1 protein isoform X23
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037545.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG GAGGAGGTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
AGCAATGAGA GTGTTGATGA CCGACTCAAC AATATGGATT GGGGCAGCCA GCAGAAGAAG 
GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA 
ACTAACGATA TTTCTCCGGA GTCATCACCG GGAGTTGGAA GACGAAGAAC AAAGACTTCA 
CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TCCCCGAGCA GGCAGAACTA 
GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCTCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCCAC TGTAAGTCCT 
CAAAAGAGAG AAATGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAGATTCGTG AGTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGACCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTCACT 
CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT CCAGAAGATT 
CTTGCCAGAG ATCCTCAGCA GGAGCCTATG GAAGAGATAG AAAATTTGAA GAAACAACAT 
GATTTATTAA AAAGGATGTT ACAGCAGCAG GAGCAGCTGA GAGCTCTACA AGGACGACAA 
GCTGCTCTGC TTGCCCTGCA GCATAAAGCT GAGCAGGCTA TAGCTGTGAT GGATGATTCT 
GAAAAGGTTG CGGAGACAAC TCCTGGCCAT CATACTGTAC CAGGCAGACA GCCGGCTCAT 
TCACCTTTGC ATCAGAGAGT ACCCATAAGA GAAACTACAG GTAGCCTATC TGGAGTCAGT 
ATCACCTCTG AACTAAACGA AGAATTGAAT GATTTAATTC AGCATTTTCA TAATCAGCTT 
CGTGATTCTC AGCCTCCAGC TGTTCCAGAC AATAGAAGAC AAGCAGAAAG TCTTTCATTA 
ACTAGGGAGA TTTCTCAGAG CAGAAATCCT TCAGTTTCAG AGCATTTACC TGATGAGAAA 
GTCCAACTTT TTAGCAAGAT GAGAGTGTTA CAAGAAAAGA AACAAAAAAT GGACAAACTT 
CTTGGAGAAC TTCATACACT TCGAGATCAG CATTTGAACA ATTCATCATT TGTGCCTTCT 
TCAGCCTCTC CACAAAGGAG TGTGGATCAG AGAAGTACAA CCACAGCTCC TTCTGCTCCT 
GTAGTCTTAA CACCAGTGGT CAATGGAGAA TCCAATAACC TCACGCCATC TGTTCCTTAT 
CCTGCTGCTT CTCTAGTTTC TCAGAATCAG AATGAAAATG AAGGCCACCT AAATCCAACT 
GAAAAACTCC AGAAGTTAAA TGAAGTTCGA AAGAGGTTGA ATGAACTAAG AGAATTAGTT 
CATTATTATG AGCAAACATC AGATATGATG ACAGATGCTG TGAATGAAAA CACAAAAGAA 
GAAGAAACTG AAGAATCAGA ATATGATTCG GAGCATGAAA ACTCGGAACC TGTTACTAAC 
ATTCGAAATC CACAAGTGGC TGCCACCTGG AACGAAGTAA ATAGTAATTC TAATGCACAG 
TGTGTTTCCA ACAATAGAGA TGGGCGAGCA GTTAATTCTA ACTGTGAAAT TAACAACAGG 
TCTGCTGCCA ACATAAGGGC TCTAAACATG CCTCCTCTTT CAGCAGATTG TCGATACAAT 
AGAGAAGGAG AACAGGAGAT GCCTGTTGCA CAAGGTCATG AGGAGGAGGA GGAGGAGGAG 
GAGGAAGCAG AAGATGAGGC CCTCAGTGGA GCTTCACTGT CTAGTCACAG CAGCAGCCTG 
ATCATTGAGA CTCCTGAAGA TGCTGAATTT GAACATAAGA TCCAGAAGCT TATGGCTGCA 
AAACTGAAAC TTAGACACTT ACAGAACCTT GTAGCCATGG TGCAGGACGA TGATGCAGCC 
AATCAAGGAG TTACTTCTGT GGAGGATTTC TTCCCAGCAG AAGACACCAA ACAAAATGCA 
AATAACACTA GAGGAAATGC CAATAAAACA CAGAAAGATG CTGGAGTAAA TGAAAAGGCA 
AGGGAGAAAT TTTATGAGGC TAAACTACAG CAGCAACAGA GAGAACTCAA ACAATTGCAG 
GAAGAAAGAA AGAAACTGAT TGAGATTCAA GAGAAAATTC AAGAATTGCA AAAGGCATGT 
CCTAACCTAC AGCTGTCAGC TGCTAGTGTG GGTAATTGTC CCACAAAAAC ACATATGCCA 
GCTGTTACTT CATCCCCAAC CGTTAATGAA AATGAAGCCA GTACAAACAA ATCTGGTTTT 
GAGCCTCATG ATTCTTCAGT GGTAGATAAT GAGGTATGGT CAGATATGCG AAGACATGAA 
ATGTTAAGGG AGGAGCTACG ACAGAGAAGA AAGCAGCTGG AAGCTCTGAT GGCTGAACAT 
CAGAGGAGGC AAGGTCTGGC TGAAACTGCA TCTCCAGTGG CTGTTTCACT GAGAAGTGAT 
GGATCTGAGA ACCTGTGTAC ACCTCAGCAA AGCAGAACAG AAAAAACAAT GGCAACTTGG 
GGAGGTTCTA CCCAGTGTGC CCTAGATGAA GAAGGAGATG AAGATGGTTA CCTTTCTGAA 
GGAATTATTC GGACAGACGA AGAGGAGGAA GAGGAGGAGC AAGATGCCAG TTCCAATGAT 
AACTTTCATA TGTATCCTCC AAACAGTGCA AATCATAATT CTTACAATGT AAAGGAAACT 
AAAAATAGGT GGAAGAACAA TCGCCCTTTT TCAGCAGATG GAAATTATCG TCCTTTAGCC 
AAGACAAGAC AGCAGAATAT CAGCATGCAG CGTCAGGAAA ACCTTCGCTG GGTGTCCGAA 
CTCTCTTATG TAGAAGAGAA AGAACAATGG CAAGAACAAA TTAATCAGCT AAAGAAACAA 
CTTGATTTCA GTGTCAATAT TTGTCAGACT TTAATGCAAG ACCAGCAGAC TCTGTCTTGT 
CTGCTACAAA CTCTTCTCAC GAGTCCTTAT AGTGTTATGC CCAGCAGTGT TGCATCCCCG 
CAAGTGCACC TTATAATGCA CCAGTTGAAT CAGTGCTATA CCCAGCTAAC GTGGCAGCAG 
AACAATGTCC AGAGGTTGAA ACAAATGCTC AGTGAACTCA TGCGCCAACA AAATCAGCAT 
CCAGAAAAAC CTGCAAGCAA GGAAGGAGGC AGTGGTACAT CACACCCTCC TTCTCCCAGT 
TTATTTTGTC CTTTCAACTT TCCAACTCAG CCTGTAAATC TGTTTAACCT ACCGGGGTTT 
ACTAATTTTT CGTCATTTGC ACCAAGTATG AATTTCAGCC CTTTATTTCC TTCTAATTTT 
GGAGATTTTT CTCAAAATAT TTCTACACCC ACTGAACAGC AGCAACCGTT AGCCCAGAAT 
CCTTCAGGGA AAACGGAATA CATGGCTTTT CCAAAACCTT TTGAAAGTAG TTCTTCTATT 
GGAGCGGAAA AACAAAGGAA TCAAAAACAG CCTGAAGAGG AGGTGGAGAA CAGTAGGACA 
CCATGGTTTT ATGACCAAGA AGGTGAAGTA GAAAAGCCAT TTCTCAAGAC TGGATTTGCA 
GTGTCTGTAG AGAAAACAAC AAATAGTAAT CGCAAAAATC AACTAGATAC CAGTAGGAGA 
AGACGCCAGT TTGATGAAGT GTCCTTAGAA AGCTTTAACA GTATGCTTGA CCCAGTAGAT 
CCAACAACAG TAACTAAAAC ATTCAAGTCA AGAAAAGCAT CTGCACAGGC CAGCCTGGCA 
TCTAAAGATA AAACTCCCAA ATCAAAGAGT AAGAAGAGGC ATTCTACTCA GCTGAAAAGC 
AGAGTGAAAA ATATCGAAAC CGGGAGTGAT TTTTCCATGT TCGAAGCTTT GCGGGATACC 
ATTTATTCTG AAGTAGCTAC GTTGATTTCT CAAAATGAAT CTCGTCCACA TTTTCTTATT 
GAACTCTTCC ATGAGCTTCA GCTACTTAAT ACAGACTATT TGAGACAGAG GGCCTTATAT 
GCATTGCAGG ACATAGTATC CAGGCATATT TCTGAGAACC ATGAAAAAGA AGGAGAAAAT 
ATAAAGTCCG TGAATTCTGG TACTTGGATA GCATCCAACT CAGAACTTAC TCCCAGTGAA 
AGCCTTGCTA CTACTGATGA TGAAACTTTT GAGAAGAACT TTGAGAGAGA AACACATAAA 
ATAAGTGAGC AAAATGATGC TGATAATGCT AGTGTATCTT CAACTTGTGA GCCTTTTGCA 
ACAGATGTTC TGGGCGACAC TGTGATTCAC TTAGATCAGG CATTAGCCAG AATGAGAGAC 
TATACGCATA TGAAGATGGA GGCTGAAAGT AGTCCAGACG TCAGGTGCAC CTGCAGGATC 
ATTGAGGATG ACGATGGTGC TGCTGCCACA ACCACGATTA GTAATAATTT AGAAGAAACT 
CCTGTTATTG AAAATCATAG TTCACAGCAA CCCGTGAGTG AAGTTGCTAC CGTCCCGTGT 
CCTAGAATTG ATACTCAGCA ACTGGACCGG CAAATTAAAG CAATTATGAA AGAAGTCATT 
CCTTTTTTGA AGGAGCACAT GGATGAAGTA TGTTCTTCTC AACTTCTAAC CTCAGTAAGA 
CGCATGGTTT TGGTCCTTAC TCAGCAAAAC AATGAGAGCA AAGAGTTTGT AAAGTTCTTT 
CATAAGCAGC TTGGAAGTAT ATTACAGGAT TCACTCGCAA AATTTGCTGG CAGAAAACTG 
AAGGATTGTG GAGAAGATCT TCTTGTAGAG ATATCTGAAG TGTTATTTAA TGAATTGGCT 
TTCTTTAAGC TCATGCAAGA TTTGGATAAT AATAGTGTAA CTGTTAAACA GAGATGCAAA 
AGGAAAATAG AAGCTGCTGG AGTGATACAG TCTTATGCAA AAGAGGCCAA AAGGATTCTT 
GAAGGAGATC ACAGTTCACC TGCTGGAGAA ATTGATGATG AAGACAAAGA CAAGGATGAG 
ACGGAAACAG TTAAGCAGAC TCAAACGTCT GAGGTTTATG ATGGTGACGG CCCCAAAAAT 
GTGAGATCTG ATGTTTCTGA TCAAGAGGAT GATGAAGAAA GTGAAGAATG TCCAGTGTCT 
ATTAATTTGT CCAAAGCTGA AACTCAGGCT TTGACTAATT ACGGAAGTGG AGAAGATGAG 
AATGAAGATG AAGAAATAGA AGAATTTGAA GAGGGACCTG TAGATGTCCA GACGTCACTT 
CAGGCTAACA CTGAAACTAC AGAAGAAAAT GAACACGATG ATCAGGTTCC ACAGCATGAT 
TTTGAAAAAT CATCAGAAAG CAAAAATGTC CCATCAGAAC AAGAACCCAC TACTAGTAAA 
GGTGACCAGA ATAGCACTCC TGTGAAGCCC TGTTACCTCA GTATCGTGGA AAATGAGCAA 
CCTTTAAATA GCCCTGTCCA GAAGGACACT CTCACTACCA TCGACTCATC CAGTCAGCCT 
AACCCTTTGC CTTTACCTTT AACTGAAATT GAAACCTTGG TGCCTAGAGT CAAAGAAGTG 
AAATCTGCTC AGGAGACTCC TGAAAGTTCT TTGGCTGGAA GTCCTGATAC TGAATCTCCA 
GTGTTAGTGA ATGACTATGA AGCAGAATCT GGTAACATAA GTCAAAAATC TGATGAAGAA 
GACTTTGTGA AAGTTGAAGA TTTACCACTT AAACTGACAA TATATTCAGA GGCAGATCTA 
AGGAAGAAAA TGGCCGAAGA AGAACAGAAA AACCACTTAT CTGGTGAAAT ATGTGCAATG 
CAGACTGAAG AATTAGCTGG GAATTCTCAG ACACTAAAAG AACCTGAAAC AGTGGGAGCC 
CAAAGTACAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025135324.1
CDS	392..6403
Translation	MATGGGPFEEGVNDQDLPNWSNESVDDRLNNMDWGSQQKKANRSSEKNKRKFGVESDKRVTNDISPESSPGVGRRRTKTSLTFPHSRYMTQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDATVSPQKREMIGSAQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIREYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLQKILARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSEKVAETTPGHHTVPGRQPAHSPLHQRVPIRETTGSLSGVSITSELNEELNDLIQHFHNQLRDSQPPAVPDNRRQAESLSLTREISQSRNPSVSEHLPDEKVQLFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSFVPSSASPQRSVDQRSTTTAPSAPVVLTPVVNGESNNLTPSVPYPAASLVSQNQNENEGHLNPTEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENTKEEETEESEYDSEHENSEPVTNIRNPQVAATWNEVNSNSNAQCVSNNRDGRAVNSNCEINNRSAANIRALNMPPLSADCRYNREGEQEMPVAQGHEEEEEEEEEAEDEALSGASLSSHSSSLIIETPEDAEFEHKIQKLMAAKLKLRHLQNLVAMVQDDDAANQGVTSVEDFFPAEDTKQNANNTRGNANKTQKDAGVNEKAREKFYEAKLQQQQRELKQLQEERKKLIEIQEKIQELQKACPNLQLSAASVGNCPTKTHMPAVTSSPTVNENEASTNKSGFEPHDSSVVDNEVWSDMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETASPVAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGIIRTDEEEEEEEQDASSNDNFHMYPPNSANHNSYNVKETKNRWKNNRPFSADGNYRPLAKTRQQNISMQRQENLRWVSELSYVEEKEQWQEQINQLKKQLDFSVNICQTLMQDQQTLSCLLQTLLTSPYSVMPSSVASPQVHLIMHQLNQCYTQLTWQQNNVQRLKQMLSELMRQQNQHPEKPASKEGGSGTSHPPSPSLFCPFNFPTQPVNLFNLPGFTNFSSFAPSMNFSPLFPSNFGDFSQNISTPTEQQQPLAQNPSGKTEYMAFPKPFESSSSIGAEKQRNQKQPEEEVENSRTPWFYDQEGEVEKPFLKTGFAVSVEKTTNSNRKNQLDTSRRRRQFDEVSLESFNSMLDPVDPTTVTKTFKSRKASAQASLASKDKTPKSKSKKRHSTQLKSRVKNIETGSDFSMFEALRDTIYSEVATLISQNESRPHFLIELFHELQLLNTDYLRQRALYALQDIVSRHISENHEKEGENIKSVNSGTWIASNSELTPSESLATTDDETFEKNFERETHKISEQNDADNASVSSTCEPFATDVLGDTVIHLDQALARMRDYTHMKMEAESSPDVRCTCRIIEDDDGAAATTTISNNLEETPVIENHSSQQPVSEVATVPCPRIDTQQLDRQIKAIMKEVIPFLKEHMDEVCSSQLLTSVRRMVLVLTQQNNESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSVTVKQRCKRKIEAAGVIQSYAKEAKRILEGDHSSPAGEIDDEDKDKDETETVKQTQTSEVYDGDGPKNVRSDVSDQEDDEESEECPVSINLSKAETQALTNYGSGEDENEDEEIEEFEEGPVDVQTSLQANTETTEENEHDDQVPQHDFEKSSESKNVPSEQEPTTSKGDQNSTPVKPCYLSIVENEQPLNSPVQKDTLTTIDSSSQPNPLPLPLTEIETLVPRVKEVKSAQETPESSLAGSPDTESPVLVNDYEAESGNISQKSDEEDFVKVEDLPLKLTIYSEADLRKKMAEEEQKNHLSGEICAMQTEELAGNSQTLKEPETVGAQST

Target ORF information:

RefSeq Version	XM_025279539.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis pericentriolar material 1 (PCM1), transcript variant X24, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025279539.1

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
AGCAATGAGA GTGTTGATGA CCGACTCAAC AATATGGATT GGGGCAGCCA GCAGAAGAAG 
GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA 
ACTAACGATA TTTCTCCGGA GTCATCACCG GGAGTTGGAA GACGAAGAAC AAAGACTTCA 
CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TCCCCGAGCA GGCAGAACTA 
GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCTCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCCAC TGTAAGTCCT 
CAAAAGAGAG AAATGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAGATTCGTG AGTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGACCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTCACT 
CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT CCAGAAGATT 
CTTGCCAGAG ATCCTCAGCA GGAGCCTATG GAAGAGATAG AAAATTTGAA GAAACAACAT 
GATTTATTAA AAAGGATGTT ACAGCAGCAG GAGCAGCTGA GAGCTCTACA AGGACGACAA 
GCTGCTCTGC TTGCCCTGCA GCATAAAGCT GAGCAGGCTA TAGCTGTGAT GGATGATTCT 
GAAAAGGTTG CGGAGACAAC TCCTGGCCAT CATACTGTAC CAGGCAGACA GCCGGCTCAT 
TCACCTTTGC ATCAGAGAGT ACCCATAAGA GAAACTACAG GTAGCCTATC TGGAGTCAGT 
ATCACCTCTG AACTAAACGA AGAATTGAAT GATTTAATTC AGCATTTTCA TAATCAGCTT 
CGTGATTCTC AGCCTCCAGC TGTTCCAGAC AATAGAAGAC AAGCAGAAAG TCTTTCATTA 
ACTAGGGAGA TTTCTCAGAG CAGAAATCCT TCAGTTTCAG AGCATTTACC TGATGAGAAA 
GTCCAACTTT TTAGCAAGAT GAGAGTGTTA CAAGAAAAGA AACAAAAAAT GGACAAACTT 
CTTGGAGAAC TTCATACACT TCGAGATCAG CATTTGAACA ATTCATCATT TGTGCCTTCT 
TCAGCCTCTC CACAAAGGAG TGTGGATCAG AGAAGTACAA CCACAGCTCC TTCTGCTCCT 
GTAGTCTTAA CACCAGTGGT CAATGGAGAA TCCAATAACC TCACGCCATC TGTTCCTTAT 
CCTGCTGCTT CTCTAGTTTC TCAGAATCAG AATGAAAATG AAGGCCACCT AAATCCAACT 
GAAAAACTCC AGAAGTTAAA TGAAGTTCGA AAGAGGTTGA ATGAACTAAG AGAATTAGTT 
CATTATTATG AGCAAACATC AGATATGATG ACAGATGCTG TGAATGAAAA CACAAAAGAA 
GAAGAAACTG AAGAATCAGA ATATGATTCG GAGCATGAAA ACTCGGAACC TGTTACTAAC 
ATTCGAAATC CACAAGTGGC TGCCACCTGG AACGAAGTAA ATAGTAATTC TAATGCACAG 
TGTGTTTCCA ACAATAGAGA TGGGCGAGCA GTTAATTCTA ACTGTGAAAT TAACAACAGG 
TCTGCTGCCA ACATAAGGGC TCTAAACATG CCTCCTCTTT CAGCAGATTG TCGATACAAT 
AGAGAAGGAG AACAGGAGAT GCCTGTTGCA CAAGGTCATG AGGAGGAGGA GGAGGAGGAG 
GAGGAAGCAG AAGATGAGGC CCTCAGTGGA GCTTCACTGT CTAGTCACAG CAGCAGCCTG 
ATCATTGAGA CTCCTGAAGA TGCTGAATTT GAACATAAGA TCCAGAAGCT TATGGCTGCA 
AAACTGAAAC TTAGACACTT ACAGAACCTT GTAGCCATGG TGCAGGACGA TGATGCAGCC 
AATCAAGGAG TTACTTCTGT GGAGGATTTC TTCCCAGCAG AAGACACCAA ACAAAATGCA 
AATAACACTA GAGGAAATGC CAATAAAACA CAGAAAGATG CTGGAGTAAA TGAAAAGGCA 
AGGGAGAAAT TTTATGAGGC TAAACTACAG CAGCAACAGA GAGAACTCAA ACAATTGCAG 
GAAGAAAGAA AGAAACTGAT TGAGATTCAA GAGAAAATTC AAGAATTGCA AAAGGCATGT 
CCTAACCTAC AGCTGTCAGC TGCTAGTGTG GGTAATTGTC CCACAAAAAC ACATATGCCA 
GCTGTTACTT CATCCCCAAC CGTTAATGAA AATGAAGCCA GTACAAACAA ATCTGGTTTT 
GAGCCTCATG ATTCTTCAGT GGTAGATAAT GAGGTATGGT CAGATATGCG AAGACATGAA 
ATGTTAAGGG AGGAGCTACG ACAGAGAAGA AAGCAGCTGG AAGCTCTGAT GGCTGAACAT 
CAGAGGAGGC AAGGTCTGGC TGAAACTGCA TCTCCAGTGG CTGTTTCACT GAGAAGTGAT 
GGATCTGAGA ACCTGTGTAC ACCTCAGCAA AGCAGAACAG AAAAAACAAT GGCAACTTGG 
GGAGGTTCTA CCCAGTGTGC CCTAGATGAA GAAGGAGATG AAGATGGTTA CCTTTCTGAA 
GGAATTATTC GGACAGACGA AGAGGAGGAA GAGGAGGAGC AAGATGCCAG TTCCAATGAT 
AACTTTCATA TGTATCCTCC AAACAGTGCA AATCATAATT CTTACAATGT AAAGGAAACT 
AAAAATAGGT GGAAGAACAA TCGCCCTTTT TCAGCAGATG GAAATTATCG TCCTTTAGCC 
AAGACAAGAC AGCAGAATAT CAGCATGCAG CGTCAGGAAA ACCTTCGCTG GGTGTCCGAA 
CTCTCTTATG TAGAAGAGAA AGAACAATGG CAAGAACAAA TTAATCAGCT AAAGAAACAA 
CTTGATTTCA GTGTCAATAT TTGTCAGACT TTAATGCAAG ACCAGCAGAC TCTGTCTTGT 
CTGCTACAAA CTCTTCTCAC GAGTCCTTAT AGTGTTATGC CCAGCAGTGT TGCATCCCCG 
CAAGTGCACC TTATAATGCA CCAGTTGAAT CAGTGCTATA CCCAGCTAAC GTGGCAGCAG 
AACAATGTCC AGAGGTTGAA ACAAATGCTC AGTGAACTCA TGCGCCAACA AAATCAGCAT 
CCAGAAAAAC CTGCAAGCAA GGAAGGAGGC AGTGGTACAT CACACCCTCC TTCTCCCAGT 
TTATTTTGTC CTTTCAACTT TCCAACTCAG CCTGTAAATC TGTTTAACCT ACCGGGGTTT 
ACTAATTTTT CGTCATTTGC ACCAAGTATG AATTTCAGCC CTTTATTTCC TTCTAATTTT 
GGAGATTTTT CTCAAAATAT TTCTACACCC ACTGAACAGC AGCAACCGTT AGCCCAGAAT 
CCTTCAGGGA AAACGGAATA CATGGCTTTT CCAAAACCTT TTGAAAGTAG TTCTTCTATT 
GGAGCGGAAA AACAAAGGAA TCAAAAACAG CCTGAAGAGG AGGTGGAGAA CAGTAGGACA 
CCATGGTTTT ATGACCAAGA AGGTGAAGTA GAAAAGCCAT TTCTCAAGAC TGGATTTGCA 
GTGTCTGTAG AGAAAACAAC AAATAGTAAT CGCAAAAATC AACTAGATAC CAGTAGGAGA 
AGACGCCAGT TTGATGAAGT GTCCTTAGAA AGCTTTAACA GTATGCTTGA CCCAGTAGAT 
CCAACAACAG TAACTAAAAC ATTCAAGTCA AGAAAAGCAT CTGCACAGGC CAGCCTGGCA 
TCTAAAGATA AAACTCCCAA ATCAAAGAGT AAGAAGAGGC ATTCTACTCA GCTGAAAAGC 
AGAGTGAAAA ATATCGAAAC CGGGAGTGAT TTTTCCATGT TCGAAGCTTT GCGGGATACC 
ATTTATTCTG AAGTAGCTAC GTTGATTTCT CAAAATGAAT CTCGTCCACA TTTTCTTATT 
GAACTCTTCC ATGAGCTTCA GCTACTTAAT ACAGACTATT TGAGACAGAG GGCCTTATAT 
GCATTGCAGG ACATAGTATC CAGGCATATT TCTGAGAACC ATGAAAAAGA AGGAGAAAAT 
ATAAAGTCCG TGAATTCTGG TACTTGGATA GCATCCAACT CAGAACTTAC TCCCAGTGAA 
AGCCTTGCTA CTACTGATGA TGAAACTTTT GAGAAGAACT TTGAGAGAGA AACACATAAA 
ATAAGTGAGC AAAATGATGC TGATAATGCT AGTGTATCTT CAACTTGTGA GCCTTTTGCA 
ACAGATGTTC TGGGCGACAC TGTGATTCAC TTAGATCAGG CATTAGCCAG AATGAGAGAC 
TATACGCATA TGAAGATGGA GGCTGAAAGT AGTCCAGACG TCAGGTGCAC CTGCAGGATC 
ATTGAGGATG ACGATGGTGC TGCTGCCACA ACCACGATTA GTAATAATTT AGAAGAAACT 
CCTGTTATTG AAAATCATAG TTCACAGCAA CCCGTGAGTG AAGTTGCTAC CGTCCCGTGT 
CCTAGAATTG ATACTCAGCA ACTGGACCGG CAAATTAAAG CAATTATGAA AGAAGTCATT 
CCTTTTTTGA AGGAGCACAT GGATGAAGTA TGTTCTTCTC AACTTCTAAC CTCAGTAAGA 
CGCATGGTTT TGGTCCTTAC TCAGCAAAAC AATGAGAGCA AAGAGTTTGT AAAGTTCTTT 
CATAAGCAGC TTGGAAGTAT ATTACAGGAT TCACTCGCAA AATTTGCTGG CAGAAAACTG 
AAGGATTGTG GAGAAGATCT TCTTGTAGAG ATATCTGAAG TGTTATTTAA TGAATTGGCT 
TTCTTTAAGC TCATGCAAGA TTTGGATAAT AATAGTGTAA CTGTTAAACA GAGATGCAAA 
AGGAAAATAG AAGCTGCTGG AGTGATACAG TCTTATGCAA AAGAGGCCAA AAGGATTCTT 
GAAGGAGATC ACAGTTCACC TGCTGGAGAA ATTGATGATG AAGACAAAGA CAAGGATGAG 
ACGGAAACAG TTAAGCAGAC TCAAACGTCT GAGGTTTATG ATGGTGACGG CCCCAAAAAT 
GTGAGATCTG ATGTTTCTGA TCAAGAGGAT GATGAAGAAA GTGAAGAATG TCCAGTGTCT 
ATTAATTTGT CCAAAGCTGA AACTCAGGCT TTGACTAATT ACGGAAGTGG AGAAGATGAG 
AATGAAGATG AAGAAATAGA AGAATTTGAA GAGGGACCTG TAGATGTCCA GACGTCACTT 
CAGGCTAACA CTGAAACTAC AGAAGAAAAT GAACACGATG ATCAGGTTCC ACAGCATGAT 
TTTGAAAAAT CATCAGAAAG CAAAAATGTC CCATCAGAAC AAGAACCCAC TACTAGTAAA 
GGTGACCAGA ATAGCACTCC TGTGAAGCCC TGTTACCTCA GTATCGTGGA AAATGAGCAA 
CCTTTAAATA GCCCTGTCCA GAAGGACACT CTCACTACCA TCGACTCATC CAGTCAGCCT 
AACCCTTTGC CTTTACCTTT AACTGAAATT GAAACCTTGG TGCCTAGAGT CAAAGAAGTG 
AAATCTGCTC AGGAGACTCC TGAAAGTTCT TTGGCTGGAA GTCCTGATAC TGAATCTCCA 
GTGTTAGTGA ATGACTATGA AGCAGAATCT GGTAACATAA GTCAAAAATC TGATGAAGAA 
GACTTTGTGA AAGTTGAAGA TTTACCACTT AAACTGACAA TATATTCAGA GGCAGATCTA 
AGGAAGAAAA TGGCCGAAGA AGAACAGAAA AACCACTTAT CTGGTGAAAT ATGTGCAATG 
CAGACTGAAG AATTAGCTGG GAATTCTCAG ACACTAAAAG AACCTGAAAC AGTGGGAGCC 
CAAAGTACAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( XM_025279544.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025135329.1 Bubalus bubalis pericentriolar material 1 (PCM1), transcript variant X25, mRNA.

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CloneID	OBu42313
Clone ID Related Accession (Same CDS sequence)	XM_025279544.1
Accession Version	XM_025279544.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6009bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	pericentriolar material 1 protein isoform X24
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037545.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG GAGGAGGTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
AGCAATGAGA GTGTTGATGA CCGACTCAAC AATATGGATT GGGGCAGCCA GCAGAAGAAG 
GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA 
ACTAACGATA TTTCTCCGGA GTCATCACCG GGAGTTGGAA GACGAAGAAC AAAGACTTCA 
CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TCCCCGAGCA GGCAGAACTA 
GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCTCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCCAC TGTAAGTCCT 
CAAAAGAGAG AAATGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAGATTCGTG AGTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGACCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTCACT 
CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT CCAGAAGATT 
CTTGCCAGAG ATCCTCAGCA GGAGCCTATG GAAGAGATAG AAAATTTGAA GAAACAACAT 
GATTTATTAA AAAGGATGTT ACAGCAGCAG GAGCAGCTGA GAGCTCTACA AGGACGACAA 
GCTGCTCTGC TTGCCCTGCA GCATAAAGCT GAGCAGGCTA TAGCTGTGAT GGATGATTCT 
GAAAAGGTTG CGGAGACAAC TCCTGGCCAT CATACTGTAC CAGGCAGACA GCCGGCTCAT 
TCACCTTTGC ATCAGAGAGT ACCCATAAGA GAAACTACAG GTAGCCTATC TGGAGTCAGT 
ATCACCTCTG AACTAAACGA AGAATTGAAT GATTTAATTC AGCATTTTCA TAATCAGCTT 
CGTGATTCTC AGCCTCCAGC TGTTCCAGAC AATAGAAGAC AAGCAGAAAG TCTTTCATTA 
ACTAGGGAGA TTTCTCAGAG CAGAAATCCT TCAGTTTCAG AGCATTTACC TGATGAGAAA 
GTCCAACTTT TTAGCAAGAT GAGAGTGTTA CAAGAAAAGA AACAAAAAAT GGACAAACTT 
CTTGGAGAAC TTCATACACT TCGAGATCAG CATTTGAACA ATTCATCATT TGTGCCTTCT 
TCAGCCTCTC CACAAAGGAG TGTGGATCAG AGAAGTACAA CCACAGCTCC TTCTGCTCCT 
GTAGTCTTAA CACCAGTGGT CAATGGAGAA TCCAATAACC TCACGCCATC TGTTCCTTAT 
CCTGCTGCTT CTCTAGTTTC TCAGAATCAG AATGAAAATG AAGGCCACCT AAATCCAACT 
GAAAAACTCC AGAAGTTAAA TGAAGTTCGA AAGAGGTTGA ATGAACTAAG AGAATTAGTT 
CATTATTATG AGCAAACATC AGATATGATG ACAGATGCTG TGAATGAAAA CACAAAAGAA 
GAAGAAACTG AAGAATCAGA ATATGATTCG GAGCATGAAA ACTCGGAACC TGTTACTAAC 
ATTCGAAATC CACAAGTGGC TGCCACCTGG AACGAAGTAA ATAGTAATTC TAATGCACAG 
TGTGTTTCCA ACAATAGAGA TGGGCGAGCA GTTAATTCTA ACTGTGAAAT TAACAACAGG 
TCTGCTGCCA ACATAAGGGC TCTAAACATG CCTCCTCTTT CAGATTGTCG ATACAATAGA 
GAAGGAGAAC AGGAGATGCC TGTTGCACAA GGTCATGAGG AGGAGGAGGA GGAGGAGGAG 
GAAGCAGAAG ATGAGGCCCT CAGTGGAGCT TCACTGTCTA GTCACAGCAG CAGCCTGATC 
ATTGAGACTC CTGAAGATGC TGAATTTGAA CATAAGATCC AGAAGCTTAT GGCTGCAAAA 
CTGAAACTTA GACACTTACA GAACCTTGTA GCCATGGTGC AGGACGATGA TGCAGCCAAT 
CAAGGAGTTA CTTCTGTGGA GGATTTCTTC CCAGCAGAAG ACACCAAACA AAATGCAAAT 
AACACTAGAG GAAATGCCAA TAAAACACAG AAAGATGCTG GAGTAAATGA AAAGGCAAGG 
GAGAAATTTT ATGAGGCTAA ACTACAGCAG CAACAGAGAG AACTCAAACA ATTGCAGGAA 
GAAAGAAAGA AACTGATTGA GATTCAAGAG AAAATTCAAG AATTGCAAAA GGCATGTCCT 
AACCTACAGC TGTCAGCTGC TAGTGTGGGT AATTGTCCCA CAAAAACACA TATGCCAGCT 
GTTACTTCAT CCCCAACCGT TAATGAAAAT GAAGCCAGTA CAAACAAATC TGGTTTTGAG 
CCTCATGATT CTTCAGTGGT AGATAATGAG GTATGGTCAG ATATGCGAAG ACATGAAATG 
TTAAGGGAGG AGCTACGACA GAGAAGAAAG CAGCTGGAAG CTCTGATGGC TGAACATCAG 
AGGAGGCAAG GTCTGGCTGA AACTGCATCT CCAGTGGCTG TTTCACTGAG AAGTGATGGA 
TCTGAGAACC TGTGTACACC TCAGCAAAGC AGAACAGAAA AAACAATGGC AACTTGGGGA 
GGTTCTACCC AGTGTGCCCT AGATGAAGAA GGAGATGAAG ATGGTTACCT TTCTGAAGGA 
ATTATTCGGA CAGACGAAGA GGAGGAAGAG GAGGAGCAAG ATGCCAGTTC CAATGATAAC 
TTTCATATGT ATCCTCCAAA CAGTGCAAAT CATAATTCTT ACAATGTAAA GGAAACTAAA 
AATAGGTGGA AGAACAATCG CCCTTTTTCA GCAGATGGAA ATTATCGTCC TTTAGCCAAG 
ACAAGACAGC AGAATATCAG CATGCAGCGT CAGGAAAACC TTCGCTGGGT GTCCGAACTC 
TCTTATGTAG AAGAGAAAGA ACAATGGCAA GAACAAATTA ATCAGCTAAA GAAACAACTT 
GATTTCAGTG TCAATATTTG TCAGACTTTA ATGCAAGACC AGCAGACTCT GTCTTGTCTG 
CTACAAACTC TTCTCACGAG TCCTTATAGT GTTATGCCCA GCAGTGTTGC ATCCCCGCAA 
GTGCACCTTA TAATGCACCA GTTGAATCAG TGCTATACCC AGCTAACGTG GCAGCAGAAC 
AATGTCCAGA GGTTGAAACA AATGCTCAGT GAACTCATGC GCCAACAAAA TCAGCATCCA 
GAAAAACCTG CAAGCAAGGA AGGAGGCAGT GGTACATCAC ACCCTCCTTC TCCCAGTTTA 
TTTTGTCCTT TCAACTTTCC AACTCAGCCT GTAAATCTGT TTAACCTACC GGGGTTTACT 
AATTTTTCGT CATTTGCACC AAGTATGAAT TTCAGCCCTT TATTTCCTTC TAATTTTGGA 
GATTTTTCTC AAAATATTTC TACACCCACT GAACAGCAGC AACCGTTAGC CCAGAATCCT 
TCAGGGAAAA CGGAATACAT GGCTTTTCCA AAACCTTTTG AAAGTAGTTC TTCTATTGGA 
GCGGAAAAAC AAAGGAATCA AAAACAGCCT GAAGAGGAGG TGGAGAACAG TAGGACACCA 
TGGTTTTATG ACCAAGAAGG TGAAGTAGAA AAGCCATTTC TCAAGACTGG ATTTGCAGTG 
TCTGTAGAGA AAACAACAAA TAGTAATCGC AAAAATCAAC TAGATACCAG TAGGAGAAGA 
CGCCAGTTTG ATGAAGTGTC CTTAGAAAGC TTTAACAGTA TGCTTGACCC AGTAGATCCA 
ACAACAGTAA CTAAAACATT CAAGTCAAGA AAAGCATCTG CACAGGCCAG CCTGGCATCT 
AAAGATAAAA CTCCCAAATC AAAGAGTAAG AAGAGGCATT CTACTCAGCT GAAAAGCAGA 
GTGAAAAATA TCGAAACCGG GAGTGATTTT TCCATGTTCG AAGCTTTGCG GGATACCATT 
TATTCTGAAG TAGCTACGTT GATTTCTCAA AATGAATCTC GTCCACATTT TCTTATTGAA 
CTCTTCCATG AGCTTCAGCT ACTTAATACA GACTATTTGA GACAGAGGGC CTTATATGCA 
TTGCAGGACA TAGTATCCAG GCATATTTCT GAGAACCATG AAAAAGAAGG AGAAAATATA 
AAGTCCGTGA ATTCTGGTAC TTGGATAGCA TCCAACTCAG AACTTACTCC CAGTGAAAGC 
CTTGCTACTA CTGATGATGA AACTTTTGAG AAGAACTTTG AGAGAGAAAC ACATAAAATA 
AGTGAGCAAA ATGATGCTGA TAATGCTAGT GTATCTTCAA CTTGTGAGCC TTTTGCAACA 
GATGTTCTGG GCGACACTGT GATTCACTTA GATCAGGCAT TAGCCAGAAT GAGAGACTAT 
ACGCATATGA AGATGGAGGC TGAAAGTAGT CCAGACGTCA GGTGCACCTG CAGGATCATT 
GAGGATGACG ATGGTGCTGC TGCCACAACC ACGATTAGTA ATAATTTAGA AGAAACTCCT 
GTTATTGAAA ATCATAGTTC ACAGCAACCC GTGAGTGAAG TTGCTACCGT CCCGTGTCCT 
AGAATTGATA CTCAGCAACT GGACCGGCAA ATTAAAGCAA TTATGAAAGA AGTCATTCCT 
TTTTTGAAGG AGCACATGGA TGAAGTATGT TCTTCTCAAC TTCTAACCTC AGTAAGACGC 
ATGGTTTTGG TCCTTACTCA GCAAAACAAT GAGAGCAAAG AGTTTGTAAA GTTCTTTCAT 
AAGCAGCTTG GAAGTATATT ACAGGATTCA CTCGCAAAAT TTGCTGGCAG AAAACTGAAG 
GATTGTGGAG AAGATCTTCT TGTAGAGATA TCTGAAGTGT TATTTAATGA ATTGGCTTTC 
TTTAAGCTCA TGCAAGATTT GGATAATAAT AGTGTAACTG TTAAACAGAG ATGCAAAAGG 
AAAATAGAAG CTGCTGGAGT GATACAGTCT TATGCAAAAG AGGCCAAAAG GATTCTTGAA 
GGAGATCACA GTTCACCTGC TGGAGAAATT GATGATGAAG ACAAAGACAA GGATGAGACG 
GAAACAGTTA AGCAGACTCA AACGTCTGAG GTTTATGATG GTGACGGCCC CAAAAATGTG 
AGATCTGATG TTTCTGATCA AGAGGATGAT GAAGAAAGTG AAGAATGTCC AGTGTCTATT 
AATTTGTCCA AAGCTGAAAC TCAGGCTTTG ACTAATTACG GAAGTGGAGA AGATGAGAAT 
GAAGATGAAG AAATAGAAGA ATTTGAAGAG GGACCTGTAG ATGTCCAGAC GTCACTTCAG 
GCTAACACTG AAACTACAGA AGAAAATGAA CACGATGATC AGGTTCCACA GCATGATTTT 
GAAAAATCAT CAGAAAGCAA AAATGTCCCA TCAGAACAAG AACCCACTAC TAGTAAAGGT 
GACCAGAATA GCACTCCTGT GAAGCCCTGT TACCTCAGTA TCGTGGAAAA TGAGCAACCT 
TTAAATAGCC CTGTCCAGAA GGACACTCTC ACTACCATCG ACTCATCCAG TCAGCCTAAC 
CCTTTGCCTT TACCTTTAAC TGAAATTGAA ACCTTGGTGC CTAGAGTCAA AGAAGTGAAA 
TCTGCTCAGG AGACTCCTGA AAGTTCTTTG GCTGGAAGTC CTGATACTGA ATCTCCAGTG 
TTAGTGAATG ACTATGAAGC AGAATCTGGT AACATAAGTC AAAAATCTGA TGAAGAAGAC 
TTTGTGAAAG TTGAAGATTT ACCACTTAAA CTGACAATAT ATTCAGAGGC AGATCTAAGG 
AAGAAAATGG CCGAAGAAGA ACAGAAAAAC CACTTATCTG GTGAAATATG TGCAATGCAG 
ACTGAAGAAT TAGCTGGGAA TTCTCAGACA CTAAAAGAAC CTGAAACAGT GGGAGCCCAA 
AGTACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025135329.1
CDS	392..6400
Translation	MATGGGPFEEGVNDQDLPNWSNESVDDRLNNMDWGSQQKKANRSSEKNKRKFGVESDKRVTNDISPESSPGVGRRRTKTSLTFPHSRYMTQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDATVSPQKREMIGSAQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIREYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLQKILARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSEKVAETTPGHHTVPGRQPAHSPLHQRVPIRETTGSLSGVSITSELNEELNDLIQHFHNQLRDSQPPAVPDNRRQAESLSLTREISQSRNPSVSEHLPDEKVQLFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSFVPSSASPQRSVDQRSTTTAPSAPVVLTPVVNGESNNLTPSVPYPAASLVSQNQNENEGHLNPTEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENTKEEETEESEYDSEHENSEPVTNIRNPQVAATWNEVNSNSNAQCVSNNRDGRAVNSNCEINNRSAANIRALNMPPLSDCRYNREGEQEMPVAQGHEEEEEEEEEAEDEALSGASLSSHSSSLIIETPEDAEFEHKIQKLMAAKLKLRHLQNLVAMVQDDDAANQGVTSVEDFFPAEDTKQNANNTRGNANKTQKDAGVNEKAREKFYEAKLQQQQRELKQLQEERKKLIEIQEKIQELQKACPNLQLSAASVGNCPTKTHMPAVTSSPTVNENEASTNKSGFEPHDSSVVDNEVWSDMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETASPVAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGIIRTDEEEEEEEQDASSNDNFHMYPPNSANHNSYNVKETKNRWKNNRPFSADGNYRPLAKTRQQNISMQRQENLRWVSELSYVEEKEQWQEQINQLKKQLDFSVNICQTLMQDQQTLSCLLQTLLTSPYSVMPSSVASPQVHLIMHQLNQCYTQLTWQQNNVQRLKQMLSELMRQQNQHPEKPASKEGGSGTSHPPSPSLFCPFNFPTQPVNLFNLPGFTNFSSFAPSMNFSPLFPSNFGDFSQNISTPTEQQQPLAQNPSGKTEYMAFPKPFESSSSIGAEKQRNQKQPEEEVENSRTPWFYDQEGEVEKPFLKTGFAVSVEKTTNSNRKNQLDTSRRRRQFDEVSLESFNSMLDPVDPTTVTKTFKSRKASAQASLASKDKTPKSKSKKRHSTQLKSRVKNIETGSDFSMFEALRDTIYSEVATLISQNESRPHFLIELFHELQLLNTDYLRQRALYALQDIVSRHISENHEKEGENIKSVNSGTWIASNSELTPSESLATTDDETFEKNFERETHKISEQNDADNASVSSTCEPFATDVLGDTVIHLDQALARMRDYTHMKMEAESSPDVRCTCRIIEDDDGAAATTTISNNLEETPVIENHSSQQPVSEVATVPCPRIDTQQLDRQIKAIMKEVIPFLKEHMDEVCSSQLLTSVRRMVLVLTQQNNESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSVTVKQRCKRKIEAAGVIQSYAKEAKRILEGDHSSPAGEIDDEDKDKDETETVKQTQTSEVYDGDGPKNVRSDVSDQEDDEESEECPVSINLSKAETQALTNYGSGEDENEDEEIEEFEEGPVDVQTSLQANTETTEENEHDDQVPQHDFEKSSESKNVPSEQEPTTSKGDQNSTPVKPCYLSIVENEQPLNSPVQKDTLTTIDSSSQPNPLPLPLTEIETLVPRVKEVKSAQETPESSLAGSPDTESPVLVNDYEAESGNISQKSDEEDFVKVEDLPLKLTIYSEADLRKKMAEEEQKNHLSGEICAMQTEELAGNSQTLKEPETVGAQST

Target ORF information:

RefSeq Version	XM_025279544.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis pericentriolar material 1 (PCM1), transcript variant X25, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025279544.1

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
AGCAATGAGA GTGTTGATGA CCGACTCAAC AATATGGATT GGGGCAGCCA GCAGAAGAAG 
GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA 
ACTAACGATA TTTCTCCGGA GTCATCACCG GGAGTTGGAA GACGAAGAAC AAAGACTTCA 
CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TCCCCGAGCA GGCAGAACTA 
GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCTCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCCAC TGTAAGTCCT 
CAAAAGAGAG AAATGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAGATTCGTG AGTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGACCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTCACT 
CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT CCAGAAGATT 
CTTGCCAGAG ATCCTCAGCA GGAGCCTATG GAAGAGATAG AAAATTTGAA GAAACAACAT 
GATTTATTAA AAAGGATGTT ACAGCAGCAG GAGCAGCTGA GAGCTCTACA AGGACGACAA 
GCTGCTCTGC TTGCCCTGCA GCATAAAGCT GAGCAGGCTA TAGCTGTGAT GGATGATTCT 
GAAAAGGTTG CGGAGACAAC TCCTGGCCAT CATACTGTAC CAGGCAGACA GCCGGCTCAT 
TCACCTTTGC ATCAGAGAGT ACCCATAAGA GAAACTACAG GTAGCCTATC TGGAGTCAGT 
ATCACCTCTG AACTAAACGA AGAATTGAAT GATTTAATTC AGCATTTTCA TAATCAGCTT 
CGTGATTCTC AGCCTCCAGC TGTTCCAGAC AATAGAAGAC AAGCAGAAAG TCTTTCATTA 
ACTAGGGAGA TTTCTCAGAG CAGAAATCCT TCAGTTTCAG AGCATTTACC TGATGAGAAA 
GTCCAACTTT TTAGCAAGAT GAGAGTGTTA CAAGAAAAGA AACAAAAAAT GGACAAACTT 
CTTGGAGAAC TTCATACACT TCGAGATCAG CATTTGAACA ATTCATCATT TGTGCCTTCT 
TCAGCCTCTC CACAAAGGAG TGTGGATCAG AGAAGTACAA CCACAGCTCC TTCTGCTCCT 
GTAGTCTTAA CACCAGTGGT CAATGGAGAA TCCAATAACC TCACGCCATC TGTTCCTTAT 
CCTGCTGCTT CTCTAGTTTC TCAGAATCAG AATGAAAATG AAGGCCACCT AAATCCAACT 
GAAAAACTCC AGAAGTTAAA TGAAGTTCGA AAGAGGTTGA ATGAACTAAG AGAATTAGTT 
CATTATTATG AGCAAACATC AGATATGATG ACAGATGCTG TGAATGAAAA CACAAAAGAA 
GAAGAAACTG AAGAATCAGA ATATGATTCG GAGCATGAAA ACTCGGAACC TGTTACTAAC 
ATTCGAAATC CACAAGTGGC TGCCACCTGG AACGAAGTAA ATAGTAATTC TAATGCACAG 
TGTGTTTCCA ACAATAGAGA TGGGCGAGCA GTTAATTCTA ACTGTGAAAT TAACAACAGG 
TCTGCTGCCA ACATAAGGGC TCTAAACATG CCTCCTCTTT CAGATTGTCG ATACAATAGA 
GAAGGAGAAC AGGAGATGCC TGTTGCACAA GGTCATGAGG AGGAGGAGGA GGAGGAGGAG 
GAAGCAGAAG ATGAGGCCCT CAGTGGAGCT TCACTGTCTA GTCACAGCAG CAGCCTGATC 
ATTGAGACTC CTGAAGATGC TGAATTTGAA CATAAGATCC AGAAGCTTAT GGCTGCAAAA 
CTGAAACTTA GACACTTACA GAACCTTGTA GCCATGGTGC AGGACGATGA TGCAGCCAAT 
CAAGGAGTTA CTTCTGTGGA GGATTTCTTC CCAGCAGAAG ACACCAAACA AAATGCAAAT 
AACACTAGAG GAAATGCCAA TAAAACACAG AAAGATGCTG GAGTAAATGA AAAGGCAAGG 
GAGAAATTTT ATGAGGCTAA ACTACAGCAG CAACAGAGAG AACTCAAACA ATTGCAGGAA 
GAAAGAAAGA AACTGATTGA GATTCAAGAG AAAATTCAAG AATTGCAAAA GGCATGTCCT 
AACCTACAGC TGTCAGCTGC TAGTGTGGGT AATTGTCCCA CAAAAACACA TATGCCAGCT 
GTTACTTCAT CCCCAACCGT TAATGAAAAT GAAGCCAGTA CAAACAAATC TGGTTTTGAG 
CCTCATGATT CTTCAGTGGT AGATAATGAG GTATGGTCAG ATATGCGAAG ACATGAAATG 
TTAAGGGAGG AGCTACGACA GAGAAGAAAG CAGCTGGAAG CTCTGATGGC TGAACATCAG 
AGGAGGCAAG GTCTGGCTGA AACTGCATCT CCAGTGGCTG TTTCACTGAG AAGTGATGGA 
TCTGAGAACC TGTGTACACC TCAGCAAAGC AGAACAGAAA AAACAATGGC AACTTGGGGA 
GGTTCTACCC AGTGTGCCCT AGATGAAGAA GGAGATGAAG ATGGTTACCT TTCTGAAGGA 
ATTATTCGGA CAGACGAAGA GGAGGAAGAG GAGGAGCAAG ATGCCAGTTC CAATGATAAC 
TTTCATATGT ATCCTCCAAA CAGTGCAAAT CATAATTCTT ACAATGTAAA GGAAACTAAA 
AATAGGTGGA AGAACAATCG CCCTTTTTCA GCAGATGGAA ATTATCGTCC TTTAGCCAAG 
ACAAGACAGC AGAATATCAG CATGCAGCGT CAGGAAAACC TTCGCTGGGT GTCCGAACTC 
TCTTATGTAG AAGAGAAAGA ACAATGGCAA GAACAAATTA ATCAGCTAAA GAAACAACTT 
GATTTCAGTG TCAATATTTG TCAGACTTTA ATGCAAGACC AGCAGACTCT GTCTTGTCTG 
CTACAAACTC TTCTCACGAG TCCTTATAGT GTTATGCCCA GCAGTGTTGC ATCCCCGCAA 
GTGCACCTTA TAATGCACCA GTTGAATCAG TGCTATACCC AGCTAACGTG GCAGCAGAAC 
AATGTCCAGA GGTTGAAACA AATGCTCAGT GAACTCATGC GCCAACAAAA TCAGCATCCA 
GAAAAACCTG CAAGCAAGGA AGGAGGCAGT GGTACATCAC ACCCTCCTTC TCCCAGTTTA 
TTTTGTCCTT TCAACTTTCC AACTCAGCCT GTAAATCTGT TTAACCTACC GGGGTTTACT 
AATTTTTCGT CATTTGCACC AAGTATGAAT TTCAGCCCTT TATTTCCTTC TAATTTTGGA 
GATTTTTCTC AAAATATTTC TACACCCACT GAACAGCAGC AACCGTTAGC CCAGAATCCT 
TCAGGGAAAA CGGAATACAT GGCTTTTCCA AAACCTTTTG AAAGTAGTTC TTCTATTGGA 
GCGGAAAAAC AAAGGAATCA AAAACAGCCT GAAGAGGAGG TGGAGAACAG TAGGACACCA 
TGGTTTTATG ACCAAGAAGG TGAAGTAGAA AAGCCATTTC TCAAGACTGG ATTTGCAGTG 
TCTGTAGAGA AAACAACAAA TAGTAATCGC AAAAATCAAC TAGATACCAG TAGGAGAAGA 
CGCCAGTTTG ATGAAGTGTC CTTAGAAAGC TTTAACAGTA TGCTTGACCC AGTAGATCCA 
ACAACAGTAA CTAAAACATT CAAGTCAAGA AAAGCATCTG CACAGGCCAG CCTGGCATCT 
AAAGATAAAA CTCCCAAATC AAAGAGTAAG AAGAGGCATT CTACTCAGCT GAAAAGCAGA 
GTGAAAAATA TCGAAACCGG GAGTGATTTT TCCATGTTCG AAGCTTTGCG GGATACCATT 
TATTCTGAAG TAGCTACGTT GATTTCTCAA AATGAATCTC GTCCACATTT TCTTATTGAA 
CTCTTCCATG AGCTTCAGCT ACTTAATACA GACTATTTGA GACAGAGGGC CTTATATGCA 
TTGCAGGACA TAGTATCCAG GCATATTTCT GAGAACCATG AAAAAGAAGG AGAAAATATA 
AAGTCCGTGA ATTCTGGTAC TTGGATAGCA TCCAACTCAG AACTTACTCC CAGTGAAAGC 
CTTGCTACTA CTGATGATGA AACTTTTGAG AAGAACTTTG AGAGAGAAAC ACATAAAATA 
AGTGAGCAAA ATGATGCTGA TAATGCTAGT GTATCTTCAA CTTGTGAGCC TTTTGCAACA 
GATGTTCTGG GCGACACTGT GATTCACTTA GATCAGGCAT TAGCCAGAAT GAGAGACTAT 
ACGCATATGA AGATGGAGGC TGAAAGTAGT CCAGACGTCA GGTGCACCTG CAGGATCATT 
GAGGATGACG ATGGTGCTGC TGCCACAACC ACGATTAGTA ATAATTTAGA AGAAACTCCT 
GTTATTGAAA ATCATAGTTC ACAGCAACCC GTGAGTGAAG TTGCTACCGT CCCGTGTCCT 
AGAATTGATA CTCAGCAACT GGACCGGCAA ATTAAAGCAA TTATGAAAGA AGTCATTCCT 
TTTTTGAAGG AGCACATGGA TGAAGTATGT TCTTCTCAAC TTCTAACCTC AGTAAGACGC 
ATGGTTTTGG TCCTTACTCA GCAAAACAAT GAGAGCAAAG AGTTTGTAAA GTTCTTTCAT 
AAGCAGCTTG GAAGTATATT ACAGGATTCA CTCGCAAAAT TTGCTGGCAG AAAACTGAAG 
GATTGTGGAG AAGATCTTCT TGTAGAGATA TCTGAAGTGT TATTTAATGA ATTGGCTTTC 
TTTAAGCTCA TGCAAGATTT GGATAATAAT AGTGTAACTG TTAAACAGAG ATGCAAAAGG 
AAAATAGAAG CTGCTGGAGT GATACAGTCT TATGCAAAAG AGGCCAAAAG GATTCTTGAA 
GGAGATCACA GTTCACCTGC TGGAGAAATT GATGATGAAG ACAAAGACAA GGATGAGACG 
GAAACAGTTA AGCAGACTCA AACGTCTGAG GTTTATGATG GTGACGGCCC CAAAAATGTG 
AGATCTGATG TTTCTGATCA AGAGGATGAT GAAGAAAGTG AAGAATGTCC AGTGTCTATT 
AATTTGTCCA AAGCTGAAAC TCAGGCTTTG ACTAATTACG GAAGTGGAGA AGATGAGAAT 
GAAGATGAAG AAATAGAAGA ATTTGAAGAG GGACCTGTAG ATGTCCAGAC GTCACTTCAG 
GCTAACACTG AAACTACAGA AGAAAATGAA CACGATGATC AGGTTCCACA GCATGATTTT 
GAAAAATCAT CAGAAAGCAA AAATGTCCCA TCAGAACAAG AACCCACTAC TAGTAAAGGT 
GACCAGAATA GCACTCCTGT GAAGCCCTGT TACCTCAGTA TCGTGGAAAA TGAGCAACCT 
TTAAATAGCC CTGTCCAGAA GGACACTCTC ACTACCATCG ACTCATCCAG TCAGCCTAAC 
CCTTTGCCTT TACCTTTAAC TGAAATTGAA ACCTTGGTGC CTAGAGTCAA AGAAGTGAAA 
TCTGCTCAGG AGACTCCTGA AAGTTCTTTG GCTGGAAGTC CTGATACTGA ATCTCCAGTG 
TTAGTGAATG ACTATGAAGC AGAATCTGGT AACATAAGTC AAAAATCTGA TGAAGAAGAC 
TTTGTGAAAG TTGAAGATTT ACCACTTAAA CTGACAATAT ATTCAGAGGC AGATCTAAGG 
AAGAAAATGG CCGAAGAAGA ACAGAAAAAC CACTTATCTG GTGAAATATG TGCAATGCAG 
ACTGAAGAAT TAGCTGGGAA TTCTCAGACA CTAAAAGAAC CTGAAACAGT GGGAGCCCAA 
AGTACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( XM_006080134.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006080196.1 Bubalus bubalis pericentriolar material 1 (PCM1), transcript variant X1, mRNA.

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CloneID	OBu33062
Clone ID Related Accession (Same CDS sequence)	XM_006080134.1 , XM_006080135.1
Accession Version	XM_006080134.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6576bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	pericentriolar material 1 protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785878.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG GAGGAGGTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
AGCAATGAGA GTGTTGATGA CCGACTCAAC AATATGGATT GGGGCAGCCA GCAGAAGAAG 
GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA 
ACTAACGATA TTTCTCCGGA GTCATCACCG GGAGTTGGAA GACGAAGAAC AAAGACTTCA 
CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TCCCCGAGCA GGCAGAACTA 
GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGATTAATAC TAACAAGAGC 
AAAGATGCCA CTGTAAGTCC TCAAAAGAGA GAAATGATTG GATCAGCACA GTGTAAAGAG 
TTGTTTGCTT CTGCTTTAAG TAATGACCTT TTGCAAAACT GTCAAGTCTC TGAAGAAGAT 
GGAAGAGGAG AACCTGCAAT GGAGAGCAGC CAGATTGTAA GCAGGCTTGT TCAGATTCGT 
GAGTATATTA CTAAAGCTAG TTCCATGCGG GAAGACCTTG TAGAGAAAAA TGAAAGATCT 
GCTAATGTTG AGCGCCTCAC TCATCTAATA GATCACCTTA AAGAACAAGA AAAGTCATAT 
ATGAAATTTC TCCAGAAGAT TCTTGCTAGA GAAAATGAGG AGGAGGATGT TCGGACTATA 
GATTCAGCTG TGGGATCTGG TTCTGTAGCT GAGAGCACAT CGCTAAACAT AGATGTGCGG 
TCTGAGGCTT CAGATGCCAC GGAGGCATCT TTTAGTCTGA GAATTCGGCC GTGCGTTGAG 
GACAAACTAG GGAATTCAGC TTCACAAGCA CAGGTTTCAG ACACTGACGT TACTACAAGT 
CCAAAAGGGA AAGGTGACAG ACCTCCTCAG CATGACAGGG AGCTGAGGCC TAATAGGAAA 
TGTAGCCGAA AACGTGGATT TCCCTCAAAG GCCAGAGATC CTCAGCAGGA GCCTATGGAA 
GAGATAGAAA ATTTGAAGAA ACAACATGAT TTATTAAAAA GGATGTTACA GCAGCAGGAG 
CAGCTGAGAG CTCTACAAGG ACGACAAGCT GCTCTGCTTG CCCTGCAGCA TAAAGCTGAG 
CAGGCTATAG CTGTGATGGA TGATTCTGAA AAGGTTGCGG AGACAACTCC TGGCCATCAT 
ACTGTACCAG GCAGACAGCC GGCTCATTCA CCTTTGCATC AGAGAGTACC CATAAGAGAA 
ACTACAGGTA GCCTATCTGG AGTCAGTATC ACCTCTGAAC TAAACGAAGA ATTGAATGAT 
TTAATTCAGC ATTTTCATAA TCAGCTTCGT GATTCTCAGC CTCCAGCTGT TCCAGACAAT 
AGAAGACAAG CAGAAAGTCT TTCATTAACT AGGGAGATTT CTCAGAGCAG AAATCCTTCA 
GTTTCAGAGC ATTTACCTGA TGAGAAAGTC CAACTTTTTA GCAAGATGAG AGTGTTACAA 
GAAAAGAAAC AAAAAATGGA CAAACTTCTT GGAGAACTTC ATACACTTCG AGATCAGCAT 
TTGAACAATT CATCATTTGT GCCTTCTTCA GCCTCTCCAC AAAGGAGTGT GGATCAGAGA 
AGTACAACCA CAGCTCCTTC TGCTCCTGTA GTCTTAACAC CAGTGGTCAA TGGAGAATCC 
AATAACCTCA CGCCATCTGT TCCTTATCCT GCTGCTTCTC TAGTTTCTCA GAATCAGAAT 
GAAAATGAAG GCCACCTAAA TCCAACTGAA AAACTCCAGA AGTTAAATGA AGTTCGAAAG 
AGGTTGAATG AACTAAGAGA ATTAGTTCAT TATTATGAGC AAACATCAGA TATGATGACA 
GATGCTGTGA ATGAAAACAC AAAAGAAGAA GAAACTGAAG AATCAGAATA TGATTCGGAG 
CATGAAAACT CGGAACCTGT TACTAACATT CGAAATCCAC AAGTGGCTGC CACCTGGAAC 
GAAGTAAATA GTAATTCTAA TGCACAGTGT GTTTCCAACA ATAGAGATGG GCGAGCAGTT 
AATTCTAACT GTGAAATTAA CAACAGGTCT GCTGCCAACA TAAGGGCTCT AAACATGCCT 
CCTCTTTCAG CAGATTGTCG ATACAATAGA GAAGGAGAAC AGGAGATGCC TGTTGCACAA 
GGTCATGAGG AGGAGGAGGA GGAGGAGGAG GAAGCAGAAG ATGAGGCCCT CAGTGGAGCT 
TCACTGTCTA GTCACAGCAG CAGCCTGATC ATTGAGACTC CTGAAGATGC TGAATTTGAA 
CATAAGATCC AGAAGCTTAT GGCTGCAAAA CTGAAACTTA GACACTTACA GAACCTTGTA 
GCCATGGTGC AGGACGATGA TGCAGCCAAT CAAGGAGTTA CTTCTGTGGA GGATTTCTTC 
CCAGCAGAAG ACACCAAACA AAATGCAAAT AACACTAGAG GAAATGCCAA TAAAACACAG 
AAAGATGCTG GAGTAAATGA AAAGGCAAGG GAGAAATTTT ATGAGGCTAA ACTACAGCAG 
CAACAGAGAG AACTCAAACA ATTGCAGGAA GAAAGAAAGA AACTGATTGA GATTCAAGAG 
AAAATTCAAG AATTGCAAAA GGCATGTCCT AACCTACAGC TGTCAGCTGC TAGTGTGGGT 
AATTGTCCCA CAAAAACACA TATGCCAGCT GTTACTTCAT CCCCAACCGT TAATGAAAAT 
GAAGCCAGTA CAAACAAATC TGGTTTTGAG CCTCATGATT CTTCAGTGGT AGATAATGAG 
GTATGGTCAG ATATGCGAAG ACATGAAATG TTAAGGGAGG AGCTACGACA GAGAAGAAAG 
CAGCTGGAAG CTCTGATGGC TGAACATCAG AGGAGGCAAG GTCTGGCTGA AACTGCATCT 
CCAGTGGCTG TTTCACTGAG AAGTGATGGA TCTGAGAACC TGTGTACACC TCAGCAAAGC 
AGAACAGAAA AAACAATGGC AACTTGGGGA GGTTCTACCC AGTGTGCCCT AGATGAAGAA 
GGAGATGAAG ATGGTTACCT TTCTGAAGGA ATTATTCGGA CAGACGAAGA GGAGGAAGAG 
GAGGAGCAAG ATGCCAGTTC CAATGATAAC TTTCATATGT ATCCTCCAAA CAGTGCAAAT 
CATAATTCTT ACAATGTAAA GGAAACTAAA AATAGGTGGA AGAACAATCG CCCTTTTTCA 
GCAGATGGAA ATTATCGTCC TTTAGCCAAG ACAAGACAGC AGAATATCAG CATGCAGCGT 
CAGGAAAACC TTCGCTGGGT GTCCGAACTC TCTTATGTAG AAGAGAAAGA ACAATGGCAA 
GAACAAATTA ATCAGCTAAA GAAACAACTT GATTTCAGTG TCAATATTTG TCAGACTTTA 
ATGCAAGACC AGCAGACTCT GTCTTGTCTG CTACAAACTC TTCTCACGAG TCCTTATAGT 
GTTATGCCCA GCAGTGTTGC ATCCCCGCAA GTGCACCTTA TAATGCACCA GTTGAATCAG 
TGCTATACCC AGCTAACGTG GCAGCAGAAC AATGTCCAGA GGTTGAAACA AATGCTCAGT 
GAACTCATGC GCCAACAAAA TCAGCATCCA GAAAAACCTG CAAGCAAGGA AGGAGGCAGT 
GGTACATCAC ACCCTCCTTC TCCCAGTTTA TTTTGTCCTT TCAACTTTCC AACTCAGCCT 
GTAAATCTGT TTAACCTACC GGGGTTTACT AATTTTTCGT CATTTGCACC AAGTATGAAT 
TTCAGCCCTT TATTTCCTTC TAATTTTGGA GATTTTTCTC AAAATATTTC TACACCCACT 
GAACAGCAGC AACCGTTAGC CCAGAATCCT TCAGGGAAAA CGGAATACAT GGCTTTTCCA 
AAACCTTTTG AAAGTAGTTC TTCTATTGGA GCGGAAAAAC AAAGGAATCA AAAACAGCCT 
GAAGAGGAGG TGGAGAACAG TAGGACACCA TGGTTTTATG ACCAAGAAGG TGAAGTAGAA 
AAGCCATTTC TCAAGACTGG ATTTGCAGTG TCTGTAGAGA AAACAACAAA TAGTAATCGC 
AAAAATCAAC TAGATACCAG TAGGAGAAGA CGCCAGTTTG ATGAAGTGTC CTTAGAAAGC 
TTTAACAGTA TGCTTGACCC AGTAGATCCA ACAACAGTAA CTAAAACATT CAAGTCAAGA 
AAAGCATCTG CACAGGCCAG CCTGGCATCT AAAGATAAAA CTCCCAAATC AAAGAGTAAG 
AAGAGGCATT CTACTCAGCT GAAAAGCAGA GTGAAAAATA TCGGGTATGA AAGTGCCAGT 
GTGTCTAGCA CATGTGAACC TTGCAAAAGT AGAAACAGAC ATTCAGCCCA AACTGAAGAG 
CCTGTTCAAG CAAAAGTACT CAGCAGAAAG AATCATGAGC AGCTGGAAAA AGTCATAAGA 
TGTAGTAGGT CTACAGAAAT ATCTTCAGCA CATGCTAGGA GAATACTACA GCAGTCTAAC 
AGAAATGCAT GCAATGAAGC GCCAGAAACC GGGAGTGATT TTTCCATGTT CGAAGCTTTG 
CGGGATACCA TTTATTCTGA AGTAGCTACG TTGATTTCTC AAAATGAATC TCGTCCACAT 
TTTCTTATTG AACTCTTCCA TGAGCTTCAG CTACTTAATA CAGACTATTT GAGACAGAGG 
GCCTTATATG CATTGCAGGA CATAGTATCC AGGCATATTT CTGAGAACCA TGAAAAAGAA 
GGAGAAAATA TAAAGTCCGT GAATTCTGGT ACTTGGATAG CATCCAACTC AGAACTTACT 
CCCAGTGAAA GCCTTGCTAC TACTGATGAT GAAACTTTTG AGAAGAACTT TGAGAGAGAA 
ACACATAAAA TAAGTGAGCA AAATGATGCT GATAATGCTA GTGTATCTTC AACTTGTGAG 
CCTTTTGCAA CAGATGTTCT GGGCGACACT GTGATTCACT TAGATCAGGC ATTAGCCAGA 
ATGAGAGACT ATACGCATAT GAAGATGGAG GCTGAAAGTA GTCCAGACGT CAGGTGCACC 
TGCAGGATCA TTGAGGATGA CGATGGTGCT GCTGCCACAA CCACGATTAG TAATAATTTA 
GAAGAAACTC CTGTTATTGA AAATCATAGT TCACAGCAAC CCGTGAGTGA AGTTGCTACC 
GTCCCGTGTC CTAGAATTGA TACTCAGCAA CTGGACCGGC AAATTAAAGC AATTATGAAA 
GAAGTCATTC CTTTTTTGAA GGAGCACATG GATGAAGTAT GTTCTTCTCA ACTTCTAACC 
TCAGTAAGAC GCATGGTTTT GGTCCTTACT CAGCAAAACA ATGAGAGCAA AGAGTTTGTA 
AAGTTCTTTC ATAAGCAGCT TGGAAGTATA TTACAGGATT CACTCGCAAA ATTTGCTGGC 
AGAAAACTGA AGGATTGTGG AGAAGATCTT CTTGTAGAGA TATCTGAAGT GTTATTTAAT 
GAATTGGCTT TCTTTAAGCT CATGCAAGAT TTGGATAATA ATAGTGTAAC TGTTAAACAG 
AGATGCAAAA GGAAAATAGA AGCTGCTGGA GTGATACAGT CTTATGCAAA AGAGGCCAAA 
AGGATTCTTG AAGGAGATCA CAGTTCACCT GCTGGAGAAA TTGATGATGA AGACAAAGAC 
AAGGATGAGA CGGAAACAGT TAAGCAGACT CAAACGTCTG AGGTTTATGA TGGTGACGGC 
CCCAAAAATG TGAGATCTGA TGTTTCTGAT CAAGAGGATG ATGAAGAAAG TGAAGAATGT 
CCAGTGTCTA TTAATTTGTC CAAAGCTGAA ACTCAGGCTT TGACTAATTA CGGAAGTGGA 
GAAGATGAGA ATGAAGATGA AGAAATAGAA GAATTTGAAG AGGGACCTGT AGATGTCCAG 
ACGTCACTTC AGGCTAACAC TGAAACTACA GAAGAAAATG AACACGATGA TCAGGTTCCA 
CAGCATGATT TTGAAAAATC ATCAGAAAGC AAAAATGTCC CATCAGAACA AGAACCCACT 
ACTAGTAAAG GTGACCAGAA TAGCACTCCT GTGAAGCCCT GTTACCTCAG TATCGTGGAA 
AATGAGCAAC CTTTAAATAG CCCTGTCCAG AAGGACACTC TCACTACCAT CGACTCATCC 
AGTCAGCCTA ACCCTTTGCC TTTACCTTTA ACTGAAATTG AAACCTTGGT GCCTAGAGTC 
AAAGAAGTGA AATCTGCTCA GGAGACTCCT GAAAGTTCTT TGGCTGGAAG TCCTGATACT 
GAATCTCCAG TGTTAGTGAA TGACTATGAA GCAGAATCTG GTAACATAAG TCAAAAATCT 
GATGAAGAAG ACTTTGTGAA AGTTGAAGAT TTACCACTTA AACTGACAAT ATATTCAGAG 
GCAGATCTAA GGAAGAAAAT GGCCGAAGAA GAACAGAAAA ACCACTTATC TGGTGAAATA 
TGTGCAATGC AGACTGAAGA ATTAGCTGGG AATTCTCAGA CACTAAAAGA ACCTGAGCAA 
GTATGTGCTC AGTCTGCCAT CTTAAGATGG AAACCCTTAC CTGTGCCTTT TAAACTGTTG 
GTGGTGTCAT CAGGGAATTA TTGTAGCAAA GTAAGTTTCT TTTCTAATAA CTTTATATTA 
AAATTGAAAG TTTTAAATGG AAGAGAACAA TATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006080196.1
CDS	113..6688
Translation	MATGGGPFEEGVNDQDLPNWSNESVDDRLNNMDWGSQQKKANRSSEKNKRKFGVESDKRVTNDISPESSPGVGRRRTKTSLTFPHSRYMTQMSVPEQAELEKLKQRINFSDLDQINTNKSKDATVSPQKREMIGSAQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIREYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLQKILARENEEEDVRTIDSAVGSGSVAESTSLNIDVRSEASDATEASFSLRIRPCVEDKLGNSASQAQVSDTDVTTSPKGKGDRPPQHDRELRPNRKCSRKRGFPSKARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSEKVAETTPGHHTVPGRQPAHSPLHQRVPIRETTGSLSGVSITSELNEELNDLIQHFHNQLRDSQPPAVPDNRRQAESLSLTREISQSRNPSVSEHLPDEKVQLFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSFVPSSASPQRSVDQRSTTTAPSAPVVLTPVVNGESNNLTPSVPYPAASLVSQNQNENEGHLNPTEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENTKEEETEESEYDSEHENSEPVTNIRNPQVAATWNEVNSNSNAQCVSNNRDGRAVNSNCEINNRSAANIRALNMPPLSADCRYNREGEQEMPVAQGHEEEEEEEEEAEDEALSGASLSSHSSSLIIETPEDAEFEHKIQKLMAAKLKLRHLQNLVAMVQDDDAANQGVTSVEDFFPAEDTKQNANNTRGNANKTQKDAGVNEKAREKFYEAKLQQQQRELKQLQEERKKLIEIQEKIQELQKACPNLQLSAASVGNCPTKTHMPAVTSSPTVNENEASTNKSGFEPHDSSVVDNEVWSDMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETASPVAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGIIRTDEEEEEEEQDASSNDNFHMYPPNSANHNSYNVKETKNRWKNNRPFSADGNYRPLAKTRQQNISMQRQENLRWVSELSYVEEKEQWQEQINQLKKQLDFSVNICQTLMQDQQTLSCLLQTLLTSPYSVMPSSVASPQVHLIMHQLNQCYTQLTWQQNNVQRLKQMLSELMRQQNQHPEKPASKEGGSGTSHPPSPSLFCPFNFPTQPVNLFNLPGFTNFSSFAPSMNFSPLFPSNFGDFSQNISTPTEQQQPLAQNPSGKTEYMAFPKPFESSSSIGAEKQRNQKQPEEEVENSRTPWFYDQEGEVEKPFLKTGFAVSVEKTTNSNRKNQLDTSRRRRQFDEVSLESFNSMLDPVDPTTVTKTFKSRKASAQASLASKDKTPKSKSKKRHSTQLKSRVKNIGYESASVSSTCEPCKSRNRHSAQTEEPVQAKVLSRKNHEQLEKVIRCSRSTEISSAHARRILQQSNRNACNEAPETGSDFSMFEALRDTIYSEVATLISQNESRPHFLIELFHELQLLNTDYLRQRALYALQDIVSRHISENHEKEGENIKSVNSGTWIASNSELTPSESLATTDDETFEKNFERETHKISEQNDADNASVSSTCEPFATDVLGDTVIHLDQALARMRDYTHMKMEAESSPDVRCTCRIIEDDDGAAATTTISNNLEETPVIENHSSQQPVSEVATVPCPRIDTQQLDRQIKAIMKEVIPFLKEHMDEVCSSQLLTSVRRMVLVLTQQNNESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSVTVKQRCKRKIEAAGVIQSYAKEAKRILEGDHSSPAGEIDDEDKDKDETETVKQTQTSEVYDGDGPKNVRSDVSDQEDDEESEECPVSINLSKAETQALTNYGSGEDENEDEEIEEFEEGPVDVQTSLQANTETTEENEHDDQVPQHDFEKSSESKNVPSEQEPTTSKGDQNSTPVKPCYLSIVENEQPLNSPVQKDTLTTIDSSSQPNPLPLPLTEIETLVPRVKEVKSAQETPESSLAGSPDTESPVLVNDYEAESGNISQKSDEEDFVKVEDLPLKLTIYSEADLRKKMAEEEQKNHLSGEICAMQTEELAGNSQTLKEPEQVCAQSAILRWKPLPVPFKLLVVSSGNYCSKVSFFSNNFILKLKVLNGREQY

Target ORF information:

RefSeq Version	XM_006080134.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis pericentriolar material 1 (PCM1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006080134.1

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
AGCAATGAGA GTGTTGATGA CCGACTCAAC AATATGGATT GGGGCAGCCA GCAGAAGAAG 
GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA 
ACTAACGATA TTTCTCCGGA GTCATCACCG GGAGTTGGAA GACGAAGAAC AAAGACTTCA 
CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TCCCCGAGCA GGCAGAACTA 
GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGATTAATAC TAACAAGAGC 
AAAGATGCCA CTGTAAGTCC TCAAAAGAGA GAAATGATTG GATCAGCACA GTGTAAAGAG 
TTGTTTGCTT CTGCTTTAAG TAATGACCTT TTGCAAAACT GTCAAGTCTC TGAAGAAGAT 
GGAAGAGGAG AACCTGCAAT GGAGAGCAGC CAGATTGTAA GCAGGCTTGT TCAGATTCGT 
GAGTATATTA CTAAAGCTAG TTCCATGCGG GAAGACCTTG TAGAGAAAAA TGAAAGATCT 
GCTAATGTTG AGCGCCTCAC TCATCTAATA GATCACCTTA AAGAACAAGA AAAGTCATAT 
ATGAAATTTC TCCAGAAGAT TCTTGCTAGA GAAAATGAGG AGGAGGATGT TCGGACTATA 
GATTCAGCTG TGGGATCTGG TTCTGTAGCT GAGAGCACAT CGCTAAACAT AGATGTGCGG 
TCTGAGGCTT CAGATGCCAC GGAGGCATCT TTTAGTCTGA GAATTCGGCC GTGCGTTGAG 
GACAAACTAG GGAATTCAGC TTCACAAGCA CAGGTTTCAG ACACTGACGT TACTACAAGT 
CCAAAAGGGA AAGGTGACAG ACCTCCTCAG CATGACAGGG AGCTGAGGCC TAATAGGAAA 
TGTAGCCGAA AACGTGGATT TCCCTCAAAG GCCAGAGATC CTCAGCAGGA GCCTATGGAA 
GAGATAGAAA ATTTGAAGAA ACAACATGAT TTATTAAAAA GGATGTTACA GCAGCAGGAG 
CAGCTGAGAG CTCTACAAGG ACGACAAGCT GCTCTGCTTG CCCTGCAGCA TAAAGCTGAG 
CAGGCTATAG CTGTGATGGA TGATTCTGAA AAGGTTGCGG AGACAACTCC TGGCCATCAT 
ACTGTACCAG GCAGACAGCC GGCTCATTCA CCTTTGCATC AGAGAGTACC CATAAGAGAA 
ACTACAGGTA GCCTATCTGG AGTCAGTATC ACCTCTGAAC TAAACGAAGA ATTGAATGAT 
TTAATTCAGC ATTTTCATAA TCAGCTTCGT GATTCTCAGC CTCCAGCTGT TCCAGACAAT 
AGAAGACAAG CAGAAAGTCT TTCATTAACT AGGGAGATTT CTCAGAGCAG AAATCCTTCA 
GTTTCAGAGC ATTTACCTGA TGAGAAAGTC CAACTTTTTA GCAAGATGAG AGTGTTACAA 
GAAAAGAAAC AAAAAATGGA CAAACTTCTT GGAGAACTTC ATACACTTCG AGATCAGCAT 
TTGAACAATT CATCATTTGT GCCTTCTTCA GCCTCTCCAC AAAGGAGTGT GGATCAGAGA 
AGTACAACCA CAGCTCCTTC TGCTCCTGTA GTCTTAACAC CAGTGGTCAA TGGAGAATCC 
AATAACCTCA CGCCATCTGT TCCTTATCCT GCTGCTTCTC TAGTTTCTCA GAATCAGAAT 
GAAAATGAAG GCCACCTAAA TCCAACTGAA AAACTCCAGA AGTTAAATGA AGTTCGAAAG 
AGGTTGAATG AACTAAGAGA ATTAGTTCAT TATTATGAGC AAACATCAGA TATGATGACA 
GATGCTGTGA ATGAAAACAC AAAAGAAGAA GAAACTGAAG AATCAGAATA TGATTCGGAG 
CATGAAAACT CGGAACCTGT TACTAACATT CGAAATCCAC AAGTGGCTGC CACCTGGAAC 
GAAGTAAATA GTAATTCTAA TGCACAGTGT GTTTCCAACA ATAGAGATGG GCGAGCAGTT 
AATTCTAACT GTGAAATTAA CAACAGGTCT GCTGCCAACA TAAGGGCTCT AAACATGCCT 
CCTCTTTCAG CAGATTGTCG ATACAATAGA GAAGGAGAAC AGGAGATGCC TGTTGCACAA 
GGTCATGAGG AGGAGGAGGA GGAGGAGGAG GAAGCAGAAG ATGAGGCCCT CAGTGGAGCT 
TCACTGTCTA GTCACAGCAG CAGCCTGATC ATTGAGACTC CTGAAGATGC TGAATTTGAA 
CATAAGATCC AGAAGCTTAT GGCTGCAAAA CTGAAACTTA GACACTTACA GAACCTTGTA 
GCCATGGTGC AGGACGATGA TGCAGCCAAT CAAGGAGTTA CTTCTGTGGA GGATTTCTTC 
CCAGCAGAAG ACACCAAACA AAATGCAAAT AACACTAGAG GAAATGCCAA TAAAACACAG 
AAAGATGCTG GAGTAAATGA AAAGGCAAGG GAGAAATTTT ATGAGGCTAA ACTACAGCAG 
CAACAGAGAG AACTCAAACA ATTGCAGGAA GAAAGAAAGA AACTGATTGA GATTCAAGAG 
AAAATTCAAG AATTGCAAAA GGCATGTCCT AACCTACAGC TGTCAGCTGC TAGTGTGGGT 
AATTGTCCCA CAAAAACACA TATGCCAGCT GTTACTTCAT CCCCAACCGT TAATGAAAAT 
GAAGCCAGTA CAAACAAATC TGGTTTTGAG CCTCATGATT CTTCAGTGGT AGATAATGAG 
GTATGGTCAG ATATGCGAAG ACATGAAATG TTAAGGGAGG AGCTACGACA GAGAAGAAAG 
CAGCTGGAAG CTCTGATGGC TGAACATCAG AGGAGGCAAG GTCTGGCTGA AACTGCATCT 
CCAGTGGCTG TTTCACTGAG AAGTGATGGA TCTGAGAACC TGTGTACACC TCAGCAAAGC 
AGAACAGAAA AAACAATGGC AACTTGGGGA GGTTCTACCC AGTGTGCCCT AGATGAAGAA 
GGAGATGAAG ATGGTTACCT TTCTGAAGGA ATTATTCGGA CAGACGAAGA GGAGGAAGAG 
GAGGAGCAAG ATGCCAGTTC CAATGATAAC TTTCATATGT ATCCTCCAAA CAGTGCAAAT 
CATAATTCTT ACAATGTAAA GGAAACTAAA AATAGGTGGA AGAACAATCG CCCTTTTTCA 
GCAGATGGAA ATTATCGTCC TTTAGCCAAG ACAAGACAGC AGAATATCAG CATGCAGCGT 
CAGGAAAACC TTCGCTGGGT GTCCGAACTC TCTTATGTAG AAGAGAAAGA ACAATGGCAA 
GAACAAATTA ATCAGCTAAA GAAACAACTT GATTTCAGTG TCAATATTTG TCAGACTTTA 
ATGCAAGACC AGCAGACTCT GTCTTGTCTG CTACAAACTC TTCTCACGAG TCCTTATAGT 
GTTATGCCCA GCAGTGTTGC ATCCCCGCAA GTGCACCTTA TAATGCACCA GTTGAATCAG 
TGCTATACCC AGCTAACGTG GCAGCAGAAC AATGTCCAGA GGTTGAAACA AATGCTCAGT 
GAACTCATGC GCCAACAAAA TCAGCATCCA GAAAAACCTG CAAGCAAGGA AGGAGGCAGT 
GGTACATCAC ACCCTCCTTC TCCCAGTTTA TTTTGTCCTT TCAACTTTCC AACTCAGCCT 
GTAAATCTGT TTAACCTACC GGGGTTTACT AATTTTTCGT CATTTGCACC AAGTATGAAT 
TTCAGCCCTT TATTTCCTTC TAATTTTGGA GATTTTTCTC AAAATATTTC TACACCCACT 
GAACAGCAGC AACCGTTAGC CCAGAATCCT TCAGGGAAAA CGGAATACAT GGCTTTTCCA 
AAACCTTTTG AAAGTAGTTC TTCTATTGGA GCGGAAAAAC AAAGGAATCA AAAACAGCCT 
GAAGAGGAGG TGGAGAACAG TAGGACACCA TGGTTTTATG ACCAAGAAGG TGAAGTAGAA 
AAGCCATTTC TCAAGACTGG ATTTGCAGTG TCTGTAGAGA AAACAACAAA TAGTAATCGC 
AAAAATCAAC TAGATACCAG TAGGAGAAGA CGCCAGTTTG ATGAAGTGTC CTTAGAAAGC 
TTTAACAGTA TGCTTGACCC AGTAGATCCA ACAACAGTAA CTAAAACATT CAAGTCAAGA 
AAAGCATCTG CACAGGCCAG CCTGGCATCT AAAGATAAAA CTCCCAAATC AAAGAGTAAG 
AAGAGGCATT CTACTCAGCT GAAAAGCAGA GTGAAAAATA TCGGGTATGA AAGTGCCAGT 
GTGTCTAGCA CATGTGAACC TTGCAAAAGT AGAAACAGAC ATTCAGCCCA AACTGAAGAG 
CCTGTTCAAG CAAAAGTACT CAGCAGAAAG AATCATGAGC AGCTGGAAAA AGTCATAAGA 
TGTAGTAGGT CTACAGAAAT ATCTTCAGCA CATGCTAGGA GAATACTACA GCAGTCTAAC 
AGAAATGCAT GCAATGAAGC GCCAGAAACC GGGAGTGATT TTTCCATGTT CGAAGCTTTG 
CGGGATACCA TTTATTCTGA AGTAGCTACG TTGATTTCTC AAAATGAATC TCGTCCACAT 
TTTCTTATTG AACTCTTCCA TGAGCTTCAG CTACTTAATA CAGACTATTT GAGACAGAGG 
GCCTTATATG CATTGCAGGA CATAGTATCC AGGCATATTT CTGAGAACCA TGAAAAAGAA 
GGAGAAAATA TAAAGTCCGT GAATTCTGGT ACTTGGATAG CATCCAACTC AGAACTTACT 
CCCAGTGAAA GCCTTGCTAC TACTGATGAT GAAACTTTTG AGAAGAACTT TGAGAGAGAA 
ACACATAAAA TAAGTGAGCA AAATGATGCT GATAATGCTA GTGTATCTTC AACTTGTGAG 
CCTTTTGCAA CAGATGTTCT GGGCGACACT GTGATTCACT TAGATCAGGC ATTAGCCAGA 
ATGAGAGACT ATACGCATAT GAAGATGGAG GCTGAAAGTA GTCCAGACGT CAGGTGCACC 
TGCAGGATCA TTGAGGATGA CGATGGTGCT GCTGCCACAA CCACGATTAG TAATAATTTA 
GAAGAAACTC CTGTTATTGA AAATCATAGT TCACAGCAAC CCGTGAGTGA AGTTGCTACC 
GTCCCGTGTC CTAGAATTGA TACTCAGCAA CTGGACCGGC AAATTAAAGC AATTATGAAA 
GAAGTCATTC CTTTTTTGAA GGAGCACATG GATGAAGTAT GTTCTTCTCA ACTTCTAACC 
TCAGTAAGAC GCATGGTTTT GGTCCTTACT CAGCAAAACA ATGAGAGCAA AGAGTTTGTA 
AAGTTCTTTC ATAAGCAGCT TGGAAGTATA TTACAGGATT CACTCGCAAA ATTTGCTGGC 
AGAAAACTGA AGGATTGTGG AGAAGATCTT CTTGTAGAGA TATCTGAAGT GTTATTTAAT 
GAATTGGCTT TCTTTAAGCT CATGCAAGAT TTGGATAATA ATAGTGTAAC TGTTAAACAG 
AGATGCAAAA GGAAAATAGA AGCTGCTGGA GTGATACAGT CTTATGCAAA AGAGGCCAAA 
AGGATTCTTG AAGGAGATCA CAGTTCACCT GCTGGAGAAA TTGATGATGA AGACAAAGAC 
AAGGATGAGA CGGAAACAGT TAAGCAGACT CAAACGTCTG AGGTTTATGA TGGTGACGGC 
CCCAAAAATG TGAGATCTGA TGTTTCTGAT CAAGAGGATG ATGAAGAAAG TGAAGAATGT 
CCAGTGTCTA TTAATTTGTC CAAAGCTGAA ACTCAGGCTT TGACTAATTA CGGAAGTGGA 
GAAGATGAGA ATGAAGATGA AGAAATAGAA GAATTTGAAG AGGGACCTGT AGATGTCCAG 
ACGTCACTTC AGGCTAACAC TGAAACTACA GAAGAAAATG AACACGATGA TCAGGTTCCA 
CAGCATGATT TTGAAAAATC ATCAGAAAGC AAAAATGTCC CATCAGAACA AGAACCCACT 
ACTAGTAAAG GTGACCAGAA TAGCACTCCT GTGAAGCCCT GTTACCTCAG TATCGTGGAA 
AATGAGCAAC CTTTAAATAG CCCTGTCCAG AAGGACACTC TCACTACCAT CGACTCATCC 
AGTCAGCCTA ACCCTTTGCC TTTACCTTTA ACTGAAATTG AAACCTTGGT GCCTAGAGTC 
AAAGAAGTGA AATCTGCTCA GGAGACTCCT GAAAGTTCTT TGGCTGGAAG TCCTGATACT 
GAATCTCCAG TGTTAGTGAA TGACTATGAA GCAGAATCTG GTAACATAAG TCAAAAATCT 
GATGAAGAAG ACTTTGTGAA AGTTGAAGAT TTACCACTTA AACTGACAAT ATATTCAGAG 
GCAGATCTAA GGAAGAAAAT GGCCGAAGAA GAACAGAAAA ACCACTTATC TGGTGAAATA 
TGTGCAATGC AGACTGAAGA ATTAGCTGGG AATTCTCAGA CACTAAAAGA ACCTGAGCAA 
GTATGTGCTC AGTCTGCCAT CTTAAGATGG AAACCCTTAC CTGTGCCTTT TAAACTGTTG 
GTGGTGTCAT CAGGGAATTA TTGTAGCAAA GTAAGTTTCT TTTCTAATAA CTTTATATTA 
AAATTGAAAG TTTTAAATGG AAGAGAACAA TATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( XM_006080135.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006080197.1 Bubalus bubalis pericentriolar material 1 (PCM1), transcript variant X2, mRNA.

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CloneID	OBu33062
Clone ID Related Accession (Same CDS sequence)	XM_006080134.1 , XM_006080135.1
Accession Version	XM_006080135.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6576bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	pericentriolar material 1 protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785878.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG GAGGAGGTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
AGCAATGAGA GTGTTGATGA CCGACTCAAC AATATGGATT GGGGCAGCCA GCAGAAGAAG 
GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA 
ACTAACGATA TTTCTCCGGA GTCATCACCG GGAGTTGGAA GACGAAGAAC AAAGACTTCA 
CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TCCCCGAGCA GGCAGAACTA 
GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGATTAATAC TAACAAGAGC 
AAAGATGCCA CTGTAAGTCC TCAAAAGAGA GAAATGATTG GATCAGCACA GTGTAAAGAG 
TTGTTTGCTT CTGCTTTAAG TAATGACCTT TTGCAAAACT GTCAAGTCTC TGAAGAAGAT 
GGAAGAGGAG AACCTGCAAT GGAGAGCAGC CAGATTGTAA GCAGGCTTGT TCAGATTCGT 
GAGTATATTA CTAAAGCTAG TTCCATGCGG GAAGACCTTG TAGAGAAAAA TGAAAGATCT 
GCTAATGTTG AGCGCCTCAC TCATCTAATA GATCACCTTA AAGAACAAGA AAAGTCATAT 
ATGAAATTTC TCCAGAAGAT TCTTGCTAGA GAAAATGAGG AGGAGGATGT TCGGACTATA 
GATTCAGCTG TGGGATCTGG TTCTGTAGCT GAGAGCACAT CGCTAAACAT AGATGTGCGG 
TCTGAGGCTT CAGATGCCAC GGAGGCATCT TTTAGTCTGA GAATTCGGCC GTGCGTTGAG 
GACAAACTAG GGAATTCAGC TTCACAAGCA CAGGTTTCAG ACACTGACGT TACTACAAGT 
CCAAAAGGGA AAGGTGACAG ACCTCCTCAG CATGACAGGG AGCTGAGGCC TAATAGGAAA 
TGTAGCCGAA AACGTGGATT TCCCTCAAAG GCCAGAGATC CTCAGCAGGA GCCTATGGAA 
GAGATAGAAA ATTTGAAGAA ACAACATGAT TTATTAAAAA GGATGTTACA GCAGCAGGAG 
CAGCTGAGAG CTCTACAAGG ACGACAAGCT GCTCTGCTTG CCCTGCAGCA TAAAGCTGAG 
CAGGCTATAG CTGTGATGGA TGATTCTGAA AAGGTTGCGG AGACAACTCC TGGCCATCAT 
ACTGTACCAG GCAGACAGCC GGCTCATTCA CCTTTGCATC AGAGAGTACC CATAAGAGAA 
ACTACAGGTA GCCTATCTGG AGTCAGTATC ACCTCTGAAC TAAACGAAGA ATTGAATGAT 
TTAATTCAGC ATTTTCATAA TCAGCTTCGT GATTCTCAGC CTCCAGCTGT TCCAGACAAT 
AGAAGACAAG CAGAAAGTCT TTCATTAACT AGGGAGATTT CTCAGAGCAG AAATCCTTCA 
GTTTCAGAGC ATTTACCTGA TGAGAAAGTC CAACTTTTTA GCAAGATGAG AGTGTTACAA 
GAAAAGAAAC AAAAAATGGA CAAACTTCTT GGAGAACTTC ATACACTTCG AGATCAGCAT 
TTGAACAATT CATCATTTGT GCCTTCTTCA GCCTCTCCAC AAAGGAGTGT GGATCAGAGA 
AGTACAACCA CAGCTCCTTC TGCTCCTGTA GTCTTAACAC CAGTGGTCAA TGGAGAATCC 
AATAACCTCA CGCCATCTGT TCCTTATCCT GCTGCTTCTC TAGTTTCTCA GAATCAGAAT 
GAAAATGAAG GCCACCTAAA TCCAACTGAA AAACTCCAGA AGTTAAATGA AGTTCGAAAG 
AGGTTGAATG AACTAAGAGA ATTAGTTCAT TATTATGAGC AAACATCAGA TATGATGACA 
GATGCTGTGA ATGAAAACAC AAAAGAAGAA GAAACTGAAG AATCAGAATA TGATTCGGAG 
CATGAAAACT CGGAACCTGT TACTAACATT CGAAATCCAC AAGTGGCTGC CACCTGGAAC 
GAAGTAAATA GTAATTCTAA TGCACAGTGT GTTTCCAACA ATAGAGATGG GCGAGCAGTT 
AATTCTAACT GTGAAATTAA CAACAGGTCT GCTGCCAACA TAAGGGCTCT AAACATGCCT 
CCTCTTTCAG CAGATTGTCG ATACAATAGA GAAGGAGAAC AGGAGATGCC TGTTGCACAA 
GGTCATGAGG AGGAGGAGGA GGAGGAGGAG GAAGCAGAAG ATGAGGCCCT CAGTGGAGCT 
TCACTGTCTA GTCACAGCAG CAGCCTGATC ATTGAGACTC CTGAAGATGC TGAATTTGAA 
CATAAGATCC AGAAGCTTAT GGCTGCAAAA CTGAAACTTA GACACTTACA GAACCTTGTA 
GCCATGGTGC AGGACGATGA TGCAGCCAAT CAAGGAGTTA CTTCTGTGGA GGATTTCTTC 
CCAGCAGAAG ACACCAAACA AAATGCAAAT AACACTAGAG GAAATGCCAA TAAAACACAG 
AAAGATGCTG GAGTAAATGA AAAGGCAAGG GAGAAATTTT ATGAGGCTAA ACTACAGCAG 
CAACAGAGAG AACTCAAACA ATTGCAGGAA GAAAGAAAGA AACTGATTGA GATTCAAGAG 
AAAATTCAAG AATTGCAAAA GGCATGTCCT AACCTACAGC TGTCAGCTGC TAGTGTGGGT 
AATTGTCCCA CAAAAACACA TATGCCAGCT GTTACTTCAT CCCCAACCGT TAATGAAAAT 
GAAGCCAGTA CAAACAAATC TGGTTTTGAG CCTCATGATT CTTCAGTGGT AGATAATGAG 
GTATGGTCAG ATATGCGAAG ACATGAAATG TTAAGGGAGG AGCTACGACA GAGAAGAAAG 
CAGCTGGAAG CTCTGATGGC TGAACATCAG AGGAGGCAAG GTCTGGCTGA AACTGCATCT 
CCAGTGGCTG TTTCACTGAG AAGTGATGGA TCTGAGAACC TGTGTACACC TCAGCAAAGC 
AGAACAGAAA AAACAATGGC AACTTGGGGA GGTTCTACCC AGTGTGCCCT AGATGAAGAA 
GGAGATGAAG ATGGTTACCT TTCTGAAGGA ATTATTCGGA CAGACGAAGA GGAGGAAGAG 
GAGGAGCAAG ATGCCAGTTC CAATGATAAC TTTCATATGT ATCCTCCAAA CAGTGCAAAT 
CATAATTCTT ACAATGTAAA GGAAACTAAA AATAGGTGGA AGAACAATCG CCCTTTTTCA 
GCAGATGGAA ATTATCGTCC TTTAGCCAAG ACAAGACAGC AGAATATCAG CATGCAGCGT 
CAGGAAAACC TTCGCTGGGT GTCCGAACTC TCTTATGTAG AAGAGAAAGA ACAATGGCAA 
GAACAAATTA ATCAGCTAAA GAAACAACTT GATTTCAGTG TCAATATTTG TCAGACTTTA 
ATGCAAGACC AGCAGACTCT GTCTTGTCTG CTACAAACTC TTCTCACGAG TCCTTATAGT 
GTTATGCCCA GCAGTGTTGC ATCCCCGCAA GTGCACCTTA TAATGCACCA GTTGAATCAG 
TGCTATACCC AGCTAACGTG GCAGCAGAAC AATGTCCAGA GGTTGAAACA AATGCTCAGT 
GAACTCATGC GCCAACAAAA TCAGCATCCA GAAAAACCTG CAAGCAAGGA AGGAGGCAGT 
GGTACATCAC ACCCTCCTTC TCCCAGTTTA TTTTGTCCTT TCAACTTTCC AACTCAGCCT 
GTAAATCTGT TTAACCTACC GGGGTTTACT AATTTTTCGT CATTTGCACC AAGTATGAAT 
TTCAGCCCTT TATTTCCTTC TAATTTTGGA GATTTTTCTC AAAATATTTC TACACCCACT 
GAACAGCAGC AACCGTTAGC CCAGAATCCT TCAGGGAAAA CGGAATACAT GGCTTTTCCA 
AAACCTTTTG AAAGTAGTTC TTCTATTGGA GCGGAAAAAC AAAGGAATCA AAAACAGCCT 
GAAGAGGAGG TGGAGAACAG TAGGACACCA TGGTTTTATG ACCAAGAAGG TGAAGTAGAA 
AAGCCATTTC TCAAGACTGG ATTTGCAGTG TCTGTAGAGA AAACAACAAA TAGTAATCGC 
AAAAATCAAC TAGATACCAG TAGGAGAAGA CGCCAGTTTG ATGAAGTGTC CTTAGAAAGC 
TTTAACAGTA TGCTTGACCC AGTAGATCCA ACAACAGTAA CTAAAACATT CAAGTCAAGA 
AAAGCATCTG CACAGGCCAG CCTGGCATCT AAAGATAAAA CTCCCAAATC AAAGAGTAAG 
AAGAGGCATT CTACTCAGCT GAAAAGCAGA GTGAAAAATA TCGGGTATGA AAGTGCCAGT 
GTGTCTAGCA CATGTGAACC TTGCAAAAGT AGAAACAGAC ATTCAGCCCA AACTGAAGAG 
CCTGTTCAAG CAAAAGTACT CAGCAGAAAG AATCATGAGC AGCTGGAAAA AGTCATAAGA 
TGTAGTAGGT CTACAGAAAT ATCTTCAGCA CATGCTAGGA GAATACTACA GCAGTCTAAC 
AGAAATGCAT GCAATGAAGC GCCAGAAACC GGGAGTGATT TTTCCATGTT CGAAGCTTTG 
CGGGATACCA TTTATTCTGA AGTAGCTACG TTGATTTCTC AAAATGAATC TCGTCCACAT 
TTTCTTATTG AACTCTTCCA TGAGCTTCAG CTACTTAATA CAGACTATTT GAGACAGAGG 
GCCTTATATG CATTGCAGGA CATAGTATCC AGGCATATTT CTGAGAACCA TGAAAAAGAA 
GGAGAAAATA TAAAGTCCGT GAATTCTGGT ACTTGGATAG CATCCAACTC AGAACTTACT 
CCCAGTGAAA GCCTTGCTAC TACTGATGAT GAAACTTTTG AGAAGAACTT TGAGAGAGAA 
ACACATAAAA TAAGTGAGCA AAATGATGCT GATAATGCTA GTGTATCTTC AACTTGTGAG 
CCTTTTGCAA CAGATGTTCT GGGCGACACT GTGATTCACT TAGATCAGGC ATTAGCCAGA 
ATGAGAGACT ATACGCATAT GAAGATGGAG GCTGAAAGTA GTCCAGACGT CAGGTGCACC 
TGCAGGATCA TTGAGGATGA CGATGGTGCT GCTGCCACAA CCACGATTAG TAATAATTTA 
GAAGAAACTC CTGTTATTGA AAATCATAGT TCACAGCAAC CCGTGAGTGA AGTTGCTACC 
GTCCCGTGTC CTAGAATTGA TACTCAGCAA CTGGACCGGC AAATTAAAGC AATTATGAAA 
GAAGTCATTC CTTTTTTGAA GGAGCACATG GATGAAGTAT GTTCTTCTCA ACTTCTAACC 
TCAGTAAGAC GCATGGTTTT GGTCCTTACT CAGCAAAACA ATGAGAGCAA AGAGTTTGTA 
AAGTTCTTTC ATAAGCAGCT TGGAAGTATA TTACAGGATT CACTCGCAAA ATTTGCTGGC 
AGAAAACTGA AGGATTGTGG AGAAGATCTT CTTGTAGAGA TATCTGAAGT GTTATTTAAT 
GAATTGGCTT TCTTTAAGCT CATGCAAGAT TTGGATAATA ATAGTGTAAC TGTTAAACAG 
AGATGCAAAA GGAAAATAGA AGCTGCTGGA GTGATACAGT CTTATGCAAA AGAGGCCAAA 
AGGATTCTTG AAGGAGATCA CAGTTCACCT GCTGGAGAAA TTGATGATGA AGACAAAGAC 
AAGGATGAGA CGGAAACAGT TAAGCAGACT CAAACGTCTG AGGTTTATGA TGGTGACGGC 
CCCAAAAATG TGAGATCTGA TGTTTCTGAT CAAGAGGATG ATGAAGAAAG TGAAGAATGT 
CCAGTGTCTA TTAATTTGTC CAAAGCTGAA ACTCAGGCTT TGACTAATTA CGGAAGTGGA 
GAAGATGAGA ATGAAGATGA AGAAATAGAA GAATTTGAAG AGGGACCTGT AGATGTCCAG 
ACGTCACTTC AGGCTAACAC TGAAACTACA GAAGAAAATG AACACGATGA TCAGGTTCCA 
CAGCATGATT TTGAAAAATC ATCAGAAAGC AAAAATGTCC CATCAGAACA AGAACCCACT 
ACTAGTAAAG GTGACCAGAA TAGCACTCCT GTGAAGCCCT GTTACCTCAG TATCGTGGAA 
AATGAGCAAC CTTTAAATAG CCCTGTCCAG AAGGACACTC TCACTACCAT CGACTCATCC 
AGTCAGCCTA ACCCTTTGCC TTTACCTTTA ACTGAAATTG AAACCTTGGT GCCTAGAGTC 
AAAGAAGTGA AATCTGCTCA GGAGACTCCT GAAAGTTCTT TGGCTGGAAG TCCTGATACT 
GAATCTCCAG TGTTAGTGAA TGACTATGAA GCAGAATCTG GTAACATAAG TCAAAAATCT 
GATGAAGAAG ACTTTGTGAA AGTTGAAGAT TTACCACTTA AACTGACAAT ATATTCAGAG 
GCAGATCTAA GGAAGAAAAT GGCCGAAGAA GAACAGAAAA ACCACTTATC TGGTGAAATA 
TGTGCAATGC AGACTGAAGA ATTAGCTGGG AATTCTCAGA CACTAAAAGA ACCTGAGCAA 
GTATGTGCTC AGTCTGCCAT CTTAAGATGG AAACCCTTAC CTGTGCCTTT TAAACTGTTG 
GTGGTGTCAT CAGGGAATTA TTGTAGCAAA GTAAGTTTCT TTTCTAATAA CTTTATATTA 
AAATTGAAAG TTTTAAATGG AAGAGAACAA TATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006080197.1
CDS	216..6791
Translation	MATGGGPFEEGVNDQDLPNWSNESVDDRLNNMDWGSQQKKANRSSEKNKRKFGVESDKRVTNDISPESSPGVGRRRTKTSLTFPHSRYMTQMSVPEQAELEKLKQRINFSDLDQINTNKSKDATVSPQKREMIGSAQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIREYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLQKILARENEEEDVRTIDSAVGSGSVAESTSLNIDVRSEASDATEASFSLRIRPCVEDKLGNSASQAQVSDTDVTTSPKGKGDRPPQHDRELRPNRKCSRKRGFPSKARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSEKVAETTPGHHTVPGRQPAHSPLHQRVPIRETTGSLSGVSITSELNEELNDLIQHFHNQLRDSQPPAVPDNRRQAESLSLTREISQSRNPSVSEHLPDEKVQLFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSFVPSSASPQRSVDQRSTTTAPSAPVVLTPVVNGESNNLTPSVPYPAASLVSQNQNENEGHLNPTEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENTKEEETEESEYDSEHENSEPVTNIRNPQVAATWNEVNSNSNAQCVSNNRDGRAVNSNCEINNRSAANIRALNMPPLSADCRYNREGEQEMPVAQGHEEEEEEEEEAEDEALSGASLSSHSSSLIIETPEDAEFEHKIQKLMAAKLKLRHLQNLVAMVQDDDAANQGVTSVEDFFPAEDTKQNANNTRGNANKTQKDAGVNEKAREKFYEAKLQQQQRELKQLQEERKKLIEIQEKIQELQKACPNLQLSAASVGNCPTKTHMPAVTSSPTVNENEASTNKSGFEPHDSSVVDNEVWSDMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETASPVAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGIIRTDEEEEEEEQDASSNDNFHMYPPNSANHNSYNVKETKNRWKNNRPFSADGNYRPLAKTRQQNISMQRQENLRWVSELSYVEEKEQWQEQINQLKKQLDFSVNICQTLMQDQQTLSCLLQTLLTSPYSVMPSSVASPQVHLIMHQLNQCYTQLTWQQNNVQRLKQMLSELMRQQNQHPEKPASKEGGSGTSHPPSPSLFCPFNFPTQPVNLFNLPGFTNFSSFAPSMNFSPLFPSNFGDFSQNISTPTEQQQPLAQNPSGKTEYMAFPKPFESSSSIGAEKQRNQKQPEEEVENSRTPWFYDQEGEVEKPFLKTGFAVSVEKTTNSNRKNQLDTSRRRRQFDEVSLESFNSMLDPVDPTTVTKTFKSRKASAQASLASKDKTPKSKSKKRHSTQLKSRVKNIGYESASVSSTCEPCKSRNRHSAQTEEPVQAKVLSRKNHEQLEKVIRCSRSTEISSAHARRILQQSNRNACNEAPETGSDFSMFEALRDTIYSEVATLISQNESRPHFLIELFHELQLLNTDYLRQRALYALQDIVSRHISENHEKEGENIKSVNSGTWIASNSELTPSESLATTDDETFEKNFERETHKISEQNDADNASVSSTCEPFATDVLGDTVIHLDQALARMRDYTHMKMEAESSPDVRCTCRIIEDDDGAAATTTISNNLEETPVIENHSSQQPVSEVATVPCPRIDTQQLDRQIKAIMKEVIPFLKEHMDEVCSSQLLTSVRRMVLVLTQQNNESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSVTVKQRCKRKIEAAGVIQSYAKEAKRILEGDHSSPAGEIDDEDKDKDETETVKQTQTSEVYDGDGPKNVRSDVSDQEDDEESEECPVSINLSKAETQALTNYGSGEDENEDEEIEEFEEGPVDVQTSLQANTETTEENEHDDQVPQHDFEKSSESKNVPSEQEPTTSKGDQNSTPVKPCYLSIVENEQPLNSPVQKDTLTTIDSSSQPNPLPLPLTEIETLVPRVKEVKSAQETPESSLAGSPDTESPVLVNDYEAESGNISQKSDEEDFVKVEDLPLKLTIYSEADLRKKMAEEEQKNHLSGEICAMQTEELAGNSQTLKEPEQVCAQSAILRWKPLPVPFKLLVVSSGNYCSKVSFFSNNFILKLKVLNGREQY

Target ORF information:

RefSeq Version	XM_006080135.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis pericentriolar material 1 (PCM1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006080135.1

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
AGCAATGAGA GTGTTGATGA CCGACTCAAC AATATGGATT GGGGCAGCCA GCAGAAGAAG 
GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA 
ACTAACGATA TTTCTCCGGA GTCATCACCG GGAGTTGGAA GACGAAGAAC AAAGACTTCA 
CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TCCCCGAGCA GGCAGAACTA 
GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGATTAATAC TAACAAGAGC 
AAAGATGCCA CTGTAAGTCC TCAAAAGAGA GAAATGATTG GATCAGCACA GTGTAAAGAG 
TTGTTTGCTT CTGCTTTAAG TAATGACCTT TTGCAAAACT GTCAAGTCTC TGAAGAAGAT 
GGAAGAGGAG AACCTGCAAT GGAGAGCAGC CAGATTGTAA GCAGGCTTGT TCAGATTCGT 
GAGTATATTA CTAAAGCTAG TTCCATGCGG GAAGACCTTG TAGAGAAAAA TGAAAGATCT 
GCTAATGTTG AGCGCCTCAC TCATCTAATA GATCACCTTA AAGAACAAGA AAAGTCATAT 
ATGAAATTTC TCCAGAAGAT TCTTGCTAGA GAAAATGAGG AGGAGGATGT TCGGACTATA 
GATTCAGCTG TGGGATCTGG TTCTGTAGCT GAGAGCACAT CGCTAAACAT AGATGTGCGG 
TCTGAGGCTT CAGATGCCAC GGAGGCATCT TTTAGTCTGA GAATTCGGCC GTGCGTTGAG 
GACAAACTAG GGAATTCAGC TTCACAAGCA CAGGTTTCAG ACACTGACGT TACTACAAGT 
CCAAAAGGGA AAGGTGACAG ACCTCCTCAG CATGACAGGG AGCTGAGGCC TAATAGGAAA 
TGTAGCCGAA AACGTGGATT TCCCTCAAAG GCCAGAGATC CTCAGCAGGA GCCTATGGAA 
GAGATAGAAA ATTTGAAGAA ACAACATGAT TTATTAAAAA GGATGTTACA GCAGCAGGAG 
CAGCTGAGAG CTCTACAAGG ACGACAAGCT GCTCTGCTTG CCCTGCAGCA TAAAGCTGAG 
CAGGCTATAG CTGTGATGGA TGATTCTGAA AAGGTTGCGG AGACAACTCC TGGCCATCAT 
ACTGTACCAG GCAGACAGCC GGCTCATTCA CCTTTGCATC AGAGAGTACC CATAAGAGAA 
ACTACAGGTA GCCTATCTGG AGTCAGTATC ACCTCTGAAC TAAACGAAGA ATTGAATGAT 
TTAATTCAGC ATTTTCATAA TCAGCTTCGT GATTCTCAGC CTCCAGCTGT TCCAGACAAT 
AGAAGACAAG CAGAAAGTCT TTCATTAACT AGGGAGATTT CTCAGAGCAG AAATCCTTCA 
GTTTCAGAGC ATTTACCTGA TGAGAAAGTC CAACTTTTTA GCAAGATGAG AGTGTTACAA 
GAAAAGAAAC AAAAAATGGA CAAACTTCTT GGAGAACTTC ATACACTTCG AGATCAGCAT 
TTGAACAATT CATCATTTGT GCCTTCTTCA GCCTCTCCAC AAAGGAGTGT GGATCAGAGA 
AGTACAACCA CAGCTCCTTC TGCTCCTGTA GTCTTAACAC CAGTGGTCAA TGGAGAATCC 
AATAACCTCA CGCCATCTGT TCCTTATCCT GCTGCTTCTC TAGTTTCTCA GAATCAGAAT 
GAAAATGAAG GCCACCTAAA TCCAACTGAA AAACTCCAGA AGTTAAATGA AGTTCGAAAG 
AGGTTGAATG AACTAAGAGA ATTAGTTCAT TATTATGAGC AAACATCAGA TATGATGACA 
GATGCTGTGA ATGAAAACAC AAAAGAAGAA GAAACTGAAG AATCAGAATA TGATTCGGAG 
CATGAAAACT CGGAACCTGT TACTAACATT CGAAATCCAC AAGTGGCTGC CACCTGGAAC 
GAAGTAAATA GTAATTCTAA TGCACAGTGT GTTTCCAACA ATAGAGATGG GCGAGCAGTT 
AATTCTAACT GTGAAATTAA CAACAGGTCT GCTGCCAACA TAAGGGCTCT AAACATGCCT 
CCTCTTTCAG CAGATTGTCG ATACAATAGA GAAGGAGAAC AGGAGATGCC TGTTGCACAA 
GGTCATGAGG AGGAGGAGGA GGAGGAGGAG GAAGCAGAAG ATGAGGCCCT CAGTGGAGCT 
TCACTGTCTA GTCACAGCAG CAGCCTGATC ATTGAGACTC CTGAAGATGC TGAATTTGAA 
CATAAGATCC AGAAGCTTAT GGCTGCAAAA CTGAAACTTA GACACTTACA GAACCTTGTA 
GCCATGGTGC AGGACGATGA TGCAGCCAAT CAAGGAGTTA CTTCTGTGGA GGATTTCTTC 
CCAGCAGAAG ACACCAAACA AAATGCAAAT AACACTAGAG GAAATGCCAA TAAAACACAG 
AAAGATGCTG GAGTAAATGA AAAGGCAAGG GAGAAATTTT ATGAGGCTAA ACTACAGCAG 
CAACAGAGAG AACTCAAACA ATTGCAGGAA GAAAGAAAGA AACTGATTGA GATTCAAGAG 
AAAATTCAAG AATTGCAAAA GGCATGTCCT AACCTACAGC TGTCAGCTGC TAGTGTGGGT 
AATTGTCCCA CAAAAACACA TATGCCAGCT GTTACTTCAT CCCCAACCGT TAATGAAAAT 
GAAGCCAGTA CAAACAAATC TGGTTTTGAG CCTCATGATT CTTCAGTGGT AGATAATGAG 
GTATGGTCAG ATATGCGAAG ACATGAAATG TTAAGGGAGG AGCTACGACA GAGAAGAAAG 
CAGCTGGAAG CTCTGATGGC TGAACATCAG AGGAGGCAAG GTCTGGCTGA AACTGCATCT 
CCAGTGGCTG TTTCACTGAG AAGTGATGGA TCTGAGAACC TGTGTACACC TCAGCAAAGC 
AGAACAGAAA AAACAATGGC AACTTGGGGA GGTTCTACCC AGTGTGCCCT AGATGAAGAA 
GGAGATGAAG ATGGTTACCT TTCTGAAGGA ATTATTCGGA CAGACGAAGA GGAGGAAGAG 
GAGGAGCAAG ATGCCAGTTC CAATGATAAC TTTCATATGT ATCCTCCAAA CAGTGCAAAT 
CATAATTCTT ACAATGTAAA GGAAACTAAA AATAGGTGGA AGAACAATCG CCCTTTTTCA 
GCAGATGGAA ATTATCGTCC TTTAGCCAAG ACAAGACAGC AGAATATCAG CATGCAGCGT 
CAGGAAAACC TTCGCTGGGT GTCCGAACTC TCTTATGTAG AAGAGAAAGA ACAATGGCAA 
GAACAAATTA ATCAGCTAAA GAAACAACTT GATTTCAGTG TCAATATTTG TCAGACTTTA 
ATGCAAGACC AGCAGACTCT GTCTTGTCTG CTACAAACTC TTCTCACGAG TCCTTATAGT 
GTTATGCCCA GCAGTGTTGC ATCCCCGCAA GTGCACCTTA TAATGCACCA GTTGAATCAG 
TGCTATACCC AGCTAACGTG GCAGCAGAAC AATGTCCAGA GGTTGAAACA AATGCTCAGT 
GAACTCATGC GCCAACAAAA TCAGCATCCA GAAAAACCTG CAAGCAAGGA AGGAGGCAGT 
GGTACATCAC ACCCTCCTTC TCCCAGTTTA TTTTGTCCTT TCAACTTTCC AACTCAGCCT 
GTAAATCTGT TTAACCTACC GGGGTTTACT AATTTTTCGT CATTTGCACC AAGTATGAAT 
TTCAGCCCTT TATTTCCTTC TAATTTTGGA GATTTTTCTC AAAATATTTC TACACCCACT 
GAACAGCAGC AACCGTTAGC CCAGAATCCT TCAGGGAAAA CGGAATACAT GGCTTTTCCA 
AAACCTTTTG AAAGTAGTTC TTCTATTGGA GCGGAAAAAC AAAGGAATCA AAAACAGCCT 
GAAGAGGAGG TGGAGAACAG TAGGACACCA TGGTTTTATG ACCAAGAAGG TGAAGTAGAA 
AAGCCATTTC TCAAGACTGG ATTTGCAGTG TCTGTAGAGA AAACAACAAA TAGTAATCGC 
AAAAATCAAC TAGATACCAG TAGGAGAAGA CGCCAGTTTG ATGAAGTGTC CTTAGAAAGC 
TTTAACAGTA TGCTTGACCC AGTAGATCCA ACAACAGTAA CTAAAACATT CAAGTCAAGA 
AAAGCATCTG CACAGGCCAG CCTGGCATCT AAAGATAAAA CTCCCAAATC AAAGAGTAAG 
AAGAGGCATT CTACTCAGCT GAAAAGCAGA GTGAAAAATA TCGGGTATGA AAGTGCCAGT 
GTGTCTAGCA CATGTGAACC TTGCAAAAGT AGAAACAGAC ATTCAGCCCA AACTGAAGAG 
CCTGTTCAAG CAAAAGTACT CAGCAGAAAG AATCATGAGC AGCTGGAAAA AGTCATAAGA 
TGTAGTAGGT CTACAGAAAT ATCTTCAGCA CATGCTAGGA GAATACTACA GCAGTCTAAC 
AGAAATGCAT GCAATGAAGC GCCAGAAACC GGGAGTGATT TTTCCATGTT CGAAGCTTTG 
CGGGATACCA TTTATTCTGA AGTAGCTACG TTGATTTCTC AAAATGAATC TCGTCCACAT 
TTTCTTATTG AACTCTTCCA TGAGCTTCAG CTACTTAATA CAGACTATTT GAGACAGAGG 
GCCTTATATG CATTGCAGGA CATAGTATCC AGGCATATTT CTGAGAACCA TGAAAAAGAA 
GGAGAAAATA TAAAGTCCGT GAATTCTGGT ACTTGGATAG CATCCAACTC AGAACTTACT 
CCCAGTGAAA GCCTTGCTAC TACTGATGAT GAAACTTTTG AGAAGAACTT TGAGAGAGAA 
ACACATAAAA TAAGTGAGCA AAATGATGCT GATAATGCTA GTGTATCTTC AACTTGTGAG 
CCTTTTGCAA CAGATGTTCT GGGCGACACT GTGATTCACT TAGATCAGGC ATTAGCCAGA 
ATGAGAGACT ATACGCATAT GAAGATGGAG GCTGAAAGTA GTCCAGACGT CAGGTGCACC 
TGCAGGATCA TTGAGGATGA CGATGGTGCT GCTGCCACAA CCACGATTAG TAATAATTTA 
GAAGAAACTC CTGTTATTGA AAATCATAGT TCACAGCAAC CCGTGAGTGA AGTTGCTACC 
GTCCCGTGTC CTAGAATTGA TACTCAGCAA CTGGACCGGC AAATTAAAGC AATTATGAAA 
GAAGTCATTC CTTTTTTGAA GGAGCACATG GATGAAGTAT GTTCTTCTCA ACTTCTAACC 
TCAGTAAGAC GCATGGTTTT GGTCCTTACT CAGCAAAACA ATGAGAGCAA AGAGTTTGTA 
AAGTTCTTTC ATAAGCAGCT TGGAAGTATA TTACAGGATT CACTCGCAAA ATTTGCTGGC 
AGAAAACTGA AGGATTGTGG AGAAGATCTT CTTGTAGAGA TATCTGAAGT GTTATTTAAT 
GAATTGGCTT TCTTTAAGCT CATGCAAGAT TTGGATAATA ATAGTGTAAC TGTTAAACAG 
AGATGCAAAA GGAAAATAGA AGCTGCTGGA GTGATACAGT CTTATGCAAA AGAGGCCAAA 
AGGATTCTTG AAGGAGATCA CAGTTCACCT GCTGGAGAAA TTGATGATGA AGACAAAGAC 
AAGGATGAGA CGGAAACAGT TAAGCAGACT CAAACGTCTG AGGTTTATGA TGGTGACGGC 
CCCAAAAATG TGAGATCTGA TGTTTCTGAT CAAGAGGATG ATGAAGAAAG TGAAGAATGT 
CCAGTGTCTA TTAATTTGTC CAAAGCTGAA ACTCAGGCTT TGACTAATTA CGGAAGTGGA 
GAAGATGAGA ATGAAGATGA AGAAATAGAA GAATTTGAAG AGGGACCTGT AGATGTCCAG 
ACGTCACTTC AGGCTAACAC TGAAACTACA GAAGAAAATG AACACGATGA TCAGGTTCCA 
CAGCATGATT TTGAAAAATC ATCAGAAAGC AAAAATGTCC CATCAGAACA AGAACCCACT 
ACTAGTAAAG GTGACCAGAA TAGCACTCCT GTGAAGCCCT GTTACCTCAG TATCGTGGAA 
AATGAGCAAC CTTTAAATAG CCCTGTCCAG AAGGACACTC TCACTACCAT CGACTCATCC 
AGTCAGCCTA ACCCTTTGCC TTTACCTTTA ACTGAAATTG AAACCTTGGT GCCTAGAGTC 
AAAGAAGTGA AATCTGCTCA GGAGACTCCT GAAAGTTCTT TGGCTGGAAG TCCTGATACT 
GAATCTCCAG TGTTAGTGAA TGACTATGAA GCAGAATCTG GTAACATAAG TCAAAAATCT 
GATGAAGAAG ACTTTGTGAA AGTTGAAGAT TTACCACTTA AACTGACAAT ATATTCAGAG 
GCAGATCTAA GGAAGAAAAT GGCCGAAGAA GAACAGAAAA ACCACTTATC TGGTGAAATA 
TGTGCAATGC AGACTGAAGA ATTAGCTGGG AATTCTCAGA CACTAAAAGA ACCTGAGCAA 
GTATGTGCTC AGTCTGCCAT CTTAAGATGG AAACCCTTAC CTGTGCCTTT TAAACTGTTG 
GTGGTGTCAT CAGGGAATTA TTGTAGCAAA GTAAGTTTCT TTTCTAATAA CTTTATATTA 
AAATTGAAAG TTTTAAATGG AAGAGAACAA TATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( XM_006080136.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006080198.1 Bubalus bubalis pericentriolar material 1 (PCM1), transcript variant X3, mRNA.

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CloneID	OBu33063
Clone ID Related Accession (Same CDS sequence)	XM_006080136.1
Accession Version	XM_006080136.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6573bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	pericentriolar material 1 protein isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785878.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG GAGGAGGTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
AGCAATGAGA GTGTTGATGA CCGACTCAAC AATATGGATT GGGGCAGCCA GCAGAAGAAG 
GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA 
ACTAACGATA TTTCTCCGGA GTCATCACCG GGAGTTGGAA GACGAAGAAC AAAGACTTCA 
CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TCCCCGAGCA GGCAGAACTA 
GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGATTAATAC TAACAAGAGC 
AAAGATGCCA CTGTAAGTCC TCAAAAGAGA GAAATGATTG GATCAGCACA GTGTAAAGAG 
TTGTTTGCTT CTGCTTTAAG TAATGACCTT TTGCAAAACT GTCAAGTCTC TGAAGAAGAT 
GGAAGAGGAG AACCTGCAAT GGAGAGCAGC CAGATTGTAA GCAGGCTTGT TCAGATTCGT 
GAGTATATTA CTAAAGCTAG TTCCATGCGG GAAGACCTTG TAGAGAAAAA TGAAAGATCT 
GCTAATGTTG AGCGCCTCAC TCATCTAATA GATCACCTTA AAGAACAAGA AAAGTCATAT 
ATGAAATTTC TCCAGAAGAT TCTTGCTAGA GAAAATGAGG AGGAGGATGT TCGGACTATA 
GATTCAGCTG TGGGATCTGG TTCTGTAGCT GAGAGCACAT CGCTAAACAT AGATGTGCGG 
TCTGAGGCTT CAGATGCCAC GGAGGCATCT TTTAGTCTGA GAATTCGGCC GTGCGTTGAG 
GACAAACTAG GGAATTCAGC TTCACAAGCA CAGGTTTCAG ACACTGACGT TACTACAAGT 
CCAAAAGGGA AAGGTGACAG ACCTCCTCAG CATGACAGGG AGCTGAGGCC TAATAGGAAA 
TGTAGCCGAA AACGTGGATT TCCCTCAAAG GCCAGAGATC CTCAGCAGGA GCCTATGGAA 
GAGATAGAAA ATTTGAAGAA ACAACATGAT TTATTAAAAA GGATGTTACA GCAGCAGGAG 
CAGCTGAGAG CTCTACAAGG ACGACAAGCT GCTCTGCTTG CCCTGCAGCA TAAAGCTGAG 
CAGGCTATAG CTGTGATGGA TGATTCTGAA AAGGTTGCGG AGACAACTCC TGGCCATCAT 
ACTGTACCAG GCAGACAGCC GGCTCATTCA CCTTTGCATC AGAGAGTACC CATAAGAGAA 
ACTACAGGTA GCCTATCTGG AGTCAGTATC ACCTCTGAAC TAAACGAAGA ATTGAATGAT 
TTAATTCAGC ATTTTCATAA TCAGCTTCGT GATTCTCAGC CTCCAGCTGT TCCAGACAAT 
AGAAGACAAG CAGAAAGTCT TTCATTAACT AGGGAGATTT CTCAGAGCAG AAATCCTTCA 
GTTTCAGAGC ATTTACCTGA TGAGAAAGTC CAACTTTTTA GCAAGATGAG AGTGTTACAA 
GAAAAGAAAC AAAAAATGGA CAAACTTCTT GGAGAACTTC ATACACTTCG AGATCAGCAT 
TTGAACAATT CATCATTTGT GCCTTCTTCA GCCTCTCCAC AAAGGAGTGT GGATCAGAGA 
AGTACAACCA CAGCTCCTTC TGCTCCTGTA GTCTTAACAC CAGTGGTCAA TGGAGAATCC 
AATAACCTCA CGCCATCTGT TCCTTATCCT GCTGCTTCTC TAGTTTCTCA GAATCAGAAT 
GAAAATGAAG GCCACCTAAA TCCAACTGAA AAACTCCAGA AGTTAAATGA AGTTCGAAAG 
AGGTTGAATG AACTAAGAGA ATTAGTTCAT TATTATGAGC AAACATCAGA TATGATGACA 
GATGCTGTGA ATGAAAACAC AAAAGAAGAA GAAACTGAAG AATCAGAATA TGATTCGGAG 
CATGAAAACT CGGAACCTGT TACTAACATT CGAAATCCAC AAGTGGCTGC CACCTGGAAC 
GAAGTAAATA GTAATTCTAA TGCACAGTGT GTTTCCAACA ATAGAGATGG GCGAGCAGTT 
AATTCTAACT GTGAAATTAA CAACAGGTCT GCTGCCAACA TAAGGGCTCT AAACATGCCT 
CCTCTTTCAG ATTGTCGATA CAATAGAGAA GGAGAACAGG AGATGCCTGT TGCACAAGGT 
CATGAGGAGG AGGAGGAGGA GGAGGAGGAA GCAGAAGATG AGGCCCTCAG TGGAGCTTCA 
CTGTCTAGTC ACAGCAGCAG CCTGATCATT GAGACTCCTG AAGATGCTGA ATTTGAACAT 
AAGATCCAGA AGCTTATGGC TGCAAAACTG AAACTTAGAC ACTTACAGAA CCTTGTAGCC 
ATGGTGCAGG ACGATGATGC AGCCAATCAA GGAGTTACTT CTGTGGAGGA TTTCTTCCCA 
GCAGAAGACA CCAAACAAAA TGCAAATAAC ACTAGAGGAA ATGCCAATAA AACACAGAAA 
GATGCTGGAG TAAATGAAAA GGCAAGGGAG AAATTTTATG AGGCTAAACT ACAGCAGCAA 
CAGAGAGAAC TCAAACAATT GCAGGAAGAA AGAAAGAAAC TGATTGAGAT TCAAGAGAAA 
ATTCAAGAAT TGCAAAAGGC ATGTCCTAAC CTACAGCTGT CAGCTGCTAG TGTGGGTAAT 
TGTCCCACAA AAACACATAT GCCAGCTGTT ACTTCATCCC CAACCGTTAA TGAAAATGAA 
GCCAGTACAA ACAAATCTGG TTTTGAGCCT CATGATTCTT CAGTGGTAGA TAATGAGGTA 
TGGTCAGATA TGCGAAGACA TGAAATGTTA AGGGAGGAGC TACGACAGAG AAGAAAGCAG 
CTGGAAGCTC TGATGGCTGA ACATCAGAGG AGGCAAGGTC TGGCTGAAAC TGCATCTCCA 
GTGGCTGTTT CACTGAGAAG TGATGGATCT GAGAACCTGT GTACACCTCA GCAAAGCAGA 
ACAGAAAAAA CAATGGCAAC TTGGGGAGGT TCTACCCAGT GTGCCCTAGA TGAAGAAGGA 
GATGAAGATG GTTACCTTTC TGAAGGAATT ATTCGGACAG ACGAAGAGGA GGAAGAGGAG 
GAGCAAGATG CCAGTTCCAA TGATAACTTT CATATGTATC CTCCAAACAG TGCAAATCAT 
AATTCTTACA ATGTAAAGGA AACTAAAAAT AGGTGGAAGA ACAATCGCCC TTTTTCAGCA 
GATGGAAATT ATCGTCCTTT AGCCAAGACA AGACAGCAGA ATATCAGCAT GCAGCGTCAG 
GAAAACCTTC GCTGGGTGTC CGAACTCTCT TATGTAGAAG AGAAAGAACA ATGGCAAGAA 
CAAATTAATC AGCTAAAGAA ACAACTTGAT TTCAGTGTCA ATATTTGTCA GACTTTAATG 
CAAGACCAGC AGACTCTGTC TTGTCTGCTA CAAACTCTTC TCACGAGTCC TTATAGTGTT 
ATGCCCAGCA GTGTTGCATC CCCGCAAGTG CACCTTATAA TGCACCAGTT GAATCAGTGC 
TATACCCAGC TAACGTGGCA GCAGAACAAT GTCCAGAGGT TGAAACAAAT GCTCAGTGAA 
CTCATGCGCC AACAAAATCA GCATCCAGAA AAACCTGCAA GCAAGGAAGG AGGCAGTGGT 
ACATCACACC CTCCTTCTCC CAGTTTATTT TGTCCTTTCA ACTTTCCAAC TCAGCCTGTA 
AATCTGTTTA ACCTACCGGG GTTTACTAAT TTTTCGTCAT TTGCACCAAG TATGAATTTC 
AGCCCTTTAT TTCCTTCTAA TTTTGGAGAT TTTTCTCAAA ATATTTCTAC ACCCACTGAA 
CAGCAGCAAC CGTTAGCCCA GAATCCTTCA GGGAAAACGG AATACATGGC TTTTCCAAAA 
CCTTTTGAAA GTAGTTCTTC TATTGGAGCG GAAAAACAAA GGAATCAAAA ACAGCCTGAA 
GAGGAGGTGG AGAACAGTAG GACACCATGG TTTTATGACC AAGAAGGTGA AGTAGAAAAG 
CCATTTCTCA AGACTGGATT TGCAGTGTCT GTAGAGAAAA CAACAAATAG TAATCGCAAA 
AATCAACTAG ATACCAGTAG GAGAAGACGC CAGTTTGATG AAGTGTCCTT AGAAAGCTTT 
AACAGTATGC TTGACCCAGT AGATCCAACA ACAGTAACTA AAACATTCAA GTCAAGAAAA 
GCATCTGCAC AGGCCAGCCT GGCATCTAAA GATAAAACTC CCAAATCAAA GAGTAAGAAG 
AGGCATTCTA CTCAGCTGAA AAGCAGAGTG AAAAATATCG GGTATGAAAG TGCCAGTGTG 
TCTAGCACAT GTGAACCTTG CAAAAGTAGA AACAGACATT CAGCCCAAAC TGAAGAGCCT 
GTTCAAGCAA AAGTACTCAG CAGAAAGAAT CATGAGCAGC TGGAAAAAGT CATAAGATGT 
AGTAGGTCTA CAGAAATATC TTCAGCACAT GCTAGGAGAA TACTACAGCA GTCTAACAGA 
AATGCATGCA ATGAAGCGCC AGAAACCGGG AGTGATTTTT CCATGTTCGA AGCTTTGCGG 
GATACCATTT ATTCTGAAGT AGCTACGTTG ATTTCTCAAA ATGAATCTCG TCCACATTTT 
CTTATTGAAC TCTTCCATGA GCTTCAGCTA CTTAATACAG ACTATTTGAG ACAGAGGGCC 
TTATATGCAT TGCAGGACAT AGTATCCAGG CATATTTCTG AGAACCATGA AAAAGAAGGA 
GAAAATATAA AGTCCGTGAA TTCTGGTACT TGGATAGCAT CCAACTCAGA ACTTACTCCC 
AGTGAAAGCC TTGCTACTAC TGATGATGAA ACTTTTGAGA AGAACTTTGA GAGAGAAACA 
CATAAAATAA GTGAGCAAAA TGATGCTGAT AATGCTAGTG TATCTTCAAC TTGTGAGCCT 
TTTGCAACAG ATGTTCTGGG CGACACTGTG ATTCACTTAG ATCAGGCATT AGCCAGAATG 
AGAGACTATA CGCATATGAA GATGGAGGCT GAAAGTAGTC CAGACGTCAG GTGCACCTGC 
AGGATCATTG AGGATGACGA TGGTGCTGCT GCCACAACCA CGATTAGTAA TAATTTAGAA 
GAAACTCCTG TTATTGAAAA TCATAGTTCA CAGCAACCCG TGAGTGAAGT TGCTACCGTC 
CCGTGTCCTA GAATTGATAC TCAGCAACTG GACCGGCAAA TTAAAGCAAT TATGAAAGAA 
GTCATTCCTT TTTTGAAGGA GCACATGGAT GAAGTATGTT CTTCTCAACT TCTAACCTCA 
GTAAGACGCA TGGTTTTGGT CCTTACTCAG CAAAACAATG AGAGCAAAGA GTTTGTAAAG 
TTCTTTCATA AGCAGCTTGG AAGTATATTA CAGGATTCAC TCGCAAAATT TGCTGGCAGA 
AAACTGAAGG ATTGTGGAGA AGATCTTCTT GTAGAGATAT CTGAAGTGTT ATTTAATGAA 
TTGGCTTTCT TTAAGCTCAT GCAAGATTTG GATAATAATA GTGTAACTGT TAAACAGAGA 
TGCAAAAGGA AAATAGAAGC TGCTGGAGTG ATACAGTCTT ATGCAAAAGA GGCCAAAAGG 
ATTCTTGAAG GAGATCACAG TTCACCTGCT GGAGAAATTG ATGATGAAGA CAAAGACAAG 
GATGAGACGG AAACAGTTAA GCAGACTCAA ACGTCTGAGG TTTATGATGG TGACGGCCCC 
AAAAATGTGA GATCTGATGT TTCTGATCAA GAGGATGATG AAGAAAGTGA AGAATGTCCA 
GTGTCTATTA ATTTGTCCAA AGCTGAAACT CAGGCTTTGA CTAATTACGG AAGTGGAGAA 
GATGAGAATG AAGATGAAGA AATAGAAGAA TTTGAAGAGG GACCTGTAGA TGTCCAGACG 
TCACTTCAGG CTAACACTGA AACTACAGAA GAAAATGAAC ACGATGATCA GGTTCCACAG 
CATGATTTTG AAAAATCATC AGAAAGCAAA AATGTCCCAT CAGAACAAGA ACCCACTACT 
AGTAAAGGTG ACCAGAATAG CACTCCTGTG AAGCCCTGTT ACCTCAGTAT CGTGGAAAAT 
GAGCAACCTT TAAATAGCCC TGTCCAGAAG GACACTCTCA CTACCATCGA CTCATCCAGT 
CAGCCTAACC CTTTGCCTTT ACCTTTAACT GAAATTGAAA CCTTGGTGCC TAGAGTCAAA 
GAAGTGAAAT CTGCTCAGGA GACTCCTGAA AGTTCTTTGG CTGGAAGTCC TGATACTGAA 
TCTCCAGTGT TAGTGAATGA CTATGAAGCA GAATCTGGTA ACATAAGTCA AAAATCTGAT 
GAAGAAGACT TTGTGAAAGT TGAAGATTTA CCACTTAAAC TGACAATATA TTCAGAGGCA 
GATCTAAGGA AGAAAATGGC CGAAGAAGAA CAGAAAAACC ACTTATCTGG TGAAATATGT 
GCAATGCAGA CTGAAGAATT AGCTGGGAAT TCTCAGACAC TAAAAGAACC TGAGCAAGTA 
TGTGCTCAGT CTGCCATCTT AAGATGGAAA CCCTTACCTG TGCCTTTTAA ACTGTTGGTG 
GTGTCATCAG GGAATTATTG TAGCAAAGTA AGTTTCTTTT CTAATAACTT TATATTAAAA 
TTGAAAGTTT TAAATGGAAG AGAACAATAT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006080198.1
CDS	113..6685
Translation	MATGGGPFEEGVNDQDLPNWSNESVDDRLNNMDWGSQQKKANRSSEKNKRKFGVESDKRVTNDISPESSPGVGRRRTKTSLTFPHSRYMTQMSVPEQAELEKLKQRINFSDLDQINTNKSKDATVSPQKREMIGSAQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIREYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLQKILARENEEEDVRTIDSAVGSGSVAESTSLNIDVRSEASDATEASFSLRIRPCVEDKLGNSASQAQVSDTDVTTSPKGKGDRPPQHDRELRPNRKCSRKRGFPSKARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSEKVAETTPGHHTVPGRQPAHSPLHQRVPIRETTGSLSGVSITSELNEELNDLIQHFHNQLRDSQPPAVPDNRRQAESLSLTREISQSRNPSVSEHLPDEKVQLFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSFVPSSASPQRSVDQRSTTTAPSAPVVLTPVVNGESNNLTPSVPYPAASLVSQNQNENEGHLNPTEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENTKEEETEESEYDSEHENSEPVTNIRNPQVAATWNEVNSNSNAQCVSNNRDGRAVNSNCEINNRSAANIRALNMPPLSDCRYNREGEQEMPVAQGHEEEEEEEEEAEDEALSGASLSSHSSSLIIETPEDAEFEHKIQKLMAAKLKLRHLQNLVAMVQDDDAANQGVTSVEDFFPAEDTKQNANNTRGNANKTQKDAGVNEKAREKFYEAKLQQQQRELKQLQEERKKLIEIQEKIQELQKACPNLQLSAASVGNCPTKTHMPAVTSSPTVNENEASTNKSGFEPHDSSVVDNEVWSDMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETASPVAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGIIRTDEEEEEEEQDASSNDNFHMYPPNSANHNSYNVKETKNRWKNNRPFSADGNYRPLAKTRQQNISMQRQENLRWVSELSYVEEKEQWQEQINQLKKQLDFSVNICQTLMQDQQTLSCLLQTLLTSPYSVMPSSVASPQVHLIMHQLNQCYTQLTWQQNNVQRLKQMLSELMRQQNQHPEKPASKEGGSGTSHPPSPSLFCPFNFPTQPVNLFNLPGFTNFSSFAPSMNFSPLFPSNFGDFSQNISTPTEQQQPLAQNPSGKTEYMAFPKPFESSSSIGAEKQRNQKQPEEEVENSRTPWFYDQEGEVEKPFLKTGFAVSVEKTTNSNRKNQLDTSRRRRQFDEVSLESFNSMLDPVDPTTVTKTFKSRKASAQASLASKDKTPKSKSKKRHSTQLKSRVKNIGYESASVSSTCEPCKSRNRHSAQTEEPVQAKVLSRKNHEQLEKVIRCSRSTEISSAHARRILQQSNRNACNEAPETGSDFSMFEALRDTIYSEVATLISQNESRPHFLIELFHELQLLNTDYLRQRALYALQDIVSRHISENHEKEGENIKSVNSGTWIASNSELTPSESLATTDDETFEKNFERETHKISEQNDADNASVSSTCEPFATDVLGDTVIHLDQALARMRDYTHMKMEAESSPDVRCTCRIIEDDDGAAATTTISNNLEETPVIENHSSQQPVSEVATVPCPRIDTQQLDRQIKAIMKEVIPFLKEHMDEVCSSQLLTSVRRMVLVLTQQNNESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSVTVKQRCKRKIEAAGVIQSYAKEAKRILEGDHSSPAGEIDDEDKDKDETETVKQTQTSEVYDGDGPKNVRSDVSDQEDDEESEECPVSINLSKAETQALTNYGSGEDENEDEEIEEFEEGPVDVQTSLQANTETTEENEHDDQVPQHDFEKSSESKNVPSEQEPTTSKGDQNSTPVKPCYLSIVENEQPLNSPVQKDTLTTIDSSSQPNPLPLPLTEIETLVPRVKEVKSAQETPESSLAGSPDTESPVLVNDYEAESGNISQKSDEEDFVKVEDLPLKLTIYSEADLRKKMAEEEQKNHLSGEICAMQTEELAGNSQTLKEPEQVCAQSAILRWKPLPVPFKLLVVSSGNYCSKVSFFSNNFILKLKVLNGREQY

Target ORF information:

RefSeq Version	XM_006080136.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis pericentriolar material 1 (PCM1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006080136.1

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
AGCAATGAGA GTGTTGATGA CCGACTCAAC AATATGGATT GGGGCAGCCA GCAGAAGAAG 
GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA 
ACTAACGATA TTTCTCCGGA GTCATCACCG GGAGTTGGAA GACGAAGAAC AAAGACTTCA 
CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TCCCCGAGCA GGCAGAACTA 
GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGATTAATAC TAACAAGAGC 
AAAGATGCCA CTGTAAGTCC TCAAAAGAGA GAAATGATTG GATCAGCACA GTGTAAAGAG 
TTGTTTGCTT CTGCTTTAAG TAATGACCTT TTGCAAAACT GTCAAGTCTC TGAAGAAGAT 
GGAAGAGGAG AACCTGCAAT GGAGAGCAGC CAGATTGTAA GCAGGCTTGT TCAGATTCGT 
GAGTATATTA CTAAAGCTAG TTCCATGCGG GAAGACCTTG TAGAGAAAAA TGAAAGATCT 
GCTAATGTTG AGCGCCTCAC TCATCTAATA GATCACCTTA AAGAACAAGA AAAGTCATAT 
ATGAAATTTC TCCAGAAGAT TCTTGCTAGA GAAAATGAGG AGGAGGATGT TCGGACTATA 
GATTCAGCTG TGGGATCTGG TTCTGTAGCT GAGAGCACAT CGCTAAACAT AGATGTGCGG 
TCTGAGGCTT CAGATGCCAC GGAGGCATCT TTTAGTCTGA GAATTCGGCC GTGCGTTGAG 
GACAAACTAG GGAATTCAGC TTCACAAGCA CAGGTTTCAG ACACTGACGT TACTACAAGT 
CCAAAAGGGA AAGGTGACAG ACCTCCTCAG CATGACAGGG AGCTGAGGCC TAATAGGAAA 
TGTAGCCGAA AACGTGGATT TCCCTCAAAG GCCAGAGATC CTCAGCAGGA GCCTATGGAA 
GAGATAGAAA ATTTGAAGAA ACAACATGAT TTATTAAAAA GGATGTTACA GCAGCAGGAG 
CAGCTGAGAG CTCTACAAGG ACGACAAGCT GCTCTGCTTG CCCTGCAGCA TAAAGCTGAG 
CAGGCTATAG CTGTGATGGA TGATTCTGAA AAGGTTGCGG AGACAACTCC TGGCCATCAT 
ACTGTACCAG GCAGACAGCC GGCTCATTCA CCTTTGCATC AGAGAGTACC CATAAGAGAA 
ACTACAGGTA GCCTATCTGG AGTCAGTATC ACCTCTGAAC TAAACGAAGA ATTGAATGAT 
TTAATTCAGC ATTTTCATAA TCAGCTTCGT GATTCTCAGC CTCCAGCTGT TCCAGACAAT 
AGAAGACAAG CAGAAAGTCT TTCATTAACT AGGGAGATTT CTCAGAGCAG AAATCCTTCA 
GTTTCAGAGC ATTTACCTGA TGAGAAAGTC CAACTTTTTA GCAAGATGAG AGTGTTACAA 
GAAAAGAAAC AAAAAATGGA CAAACTTCTT GGAGAACTTC ATACACTTCG AGATCAGCAT 
TTGAACAATT CATCATTTGT GCCTTCTTCA GCCTCTCCAC AAAGGAGTGT GGATCAGAGA 
AGTACAACCA CAGCTCCTTC TGCTCCTGTA GTCTTAACAC CAGTGGTCAA TGGAGAATCC 
AATAACCTCA CGCCATCTGT TCCTTATCCT GCTGCTTCTC TAGTTTCTCA GAATCAGAAT 
GAAAATGAAG GCCACCTAAA TCCAACTGAA AAACTCCAGA AGTTAAATGA AGTTCGAAAG 
AGGTTGAATG AACTAAGAGA ATTAGTTCAT TATTATGAGC AAACATCAGA TATGATGACA 
GATGCTGTGA ATGAAAACAC AAAAGAAGAA GAAACTGAAG AATCAGAATA TGATTCGGAG 
CATGAAAACT CGGAACCTGT TACTAACATT CGAAATCCAC AAGTGGCTGC CACCTGGAAC 
GAAGTAAATA GTAATTCTAA TGCACAGTGT GTTTCCAACA ATAGAGATGG GCGAGCAGTT 
AATTCTAACT GTGAAATTAA CAACAGGTCT GCTGCCAACA TAAGGGCTCT AAACATGCCT 
CCTCTTTCAG ATTGTCGATA CAATAGAGAA GGAGAACAGG AGATGCCTGT TGCACAAGGT 
CATGAGGAGG AGGAGGAGGA GGAGGAGGAA GCAGAAGATG AGGCCCTCAG TGGAGCTTCA 
CTGTCTAGTC ACAGCAGCAG CCTGATCATT GAGACTCCTG AAGATGCTGA ATTTGAACAT 
AAGATCCAGA AGCTTATGGC TGCAAAACTG AAACTTAGAC ACTTACAGAA CCTTGTAGCC 
ATGGTGCAGG ACGATGATGC AGCCAATCAA GGAGTTACTT CTGTGGAGGA TTTCTTCCCA 
GCAGAAGACA CCAAACAAAA TGCAAATAAC ACTAGAGGAA ATGCCAATAA AACACAGAAA 
GATGCTGGAG TAAATGAAAA GGCAAGGGAG AAATTTTATG AGGCTAAACT ACAGCAGCAA 
CAGAGAGAAC TCAAACAATT GCAGGAAGAA AGAAAGAAAC TGATTGAGAT TCAAGAGAAA 
ATTCAAGAAT TGCAAAAGGC ATGTCCTAAC CTACAGCTGT CAGCTGCTAG TGTGGGTAAT 
TGTCCCACAA AAACACATAT GCCAGCTGTT ACTTCATCCC CAACCGTTAA TGAAAATGAA 
GCCAGTACAA ACAAATCTGG TTTTGAGCCT CATGATTCTT CAGTGGTAGA TAATGAGGTA 
TGGTCAGATA TGCGAAGACA TGAAATGTTA AGGGAGGAGC TACGACAGAG AAGAAAGCAG 
CTGGAAGCTC TGATGGCTGA ACATCAGAGG AGGCAAGGTC TGGCTGAAAC TGCATCTCCA 
GTGGCTGTTT CACTGAGAAG TGATGGATCT GAGAACCTGT GTACACCTCA GCAAAGCAGA 
ACAGAAAAAA CAATGGCAAC TTGGGGAGGT TCTACCCAGT GTGCCCTAGA TGAAGAAGGA 
GATGAAGATG GTTACCTTTC TGAAGGAATT ATTCGGACAG ACGAAGAGGA GGAAGAGGAG 
GAGCAAGATG CCAGTTCCAA TGATAACTTT CATATGTATC CTCCAAACAG TGCAAATCAT 
AATTCTTACA ATGTAAAGGA AACTAAAAAT AGGTGGAAGA ACAATCGCCC TTTTTCAGCA 
GATGGAAATT ATCGTCCTTT AGCCAAGACA AGACAGCAGA ATATCAGCAT GCAGCGTCAG 
GAAAACCTTC GCTGGGTGTC CGAACTCTCT TATGTAGAAG AGAAAGAACA ATGGCAAGAA 
CAAATTAATC AGCTAAAGAA ACAACTTGAT TTCAGTGTCA ATATTTGTCA GACTTTAATG 
CAAGACCAGC AGACTCTGTC TTGTCTGCTA CAAACTCTTC TCACGAGTCC TTATAGTGTT 
ATGCCCAGCA GTGTTGCATC CCCGCAAGTG CACCTTATAA TGCACCAGTT GAATCAGTGC 
TATACCCAGC TAACGTGGCA GCAGAACAAT GTCCAGAGGT TGAAACAAAT GCTCAGTGAA 
CTCATGCGCC AACAAAATCA GCATCCAGAA AAACCTGCAA GCAAGGAAGG AGGCAGTGGT 
ACATCACACC CTCCTTCTCC CAGTTTATTT TGTCCTTTCA ACTTTCCAAC TCAGCCTGTA 
AATCTGTTTA ACCTACCGGG GTTTACTAAT TTTTCGTCAT TTGCACCAAG TATGAATTTC 
AGCCCTTTAT TTCCTTCTAA TTTTGGAGAT TTTTCTCAAA ATATTTCTAC ACCCACTGAA 
CAGCAGCAAC CGTTAGCCCA GAATCCTTCA GGGAAAACGG AATACATGGC TTTTCCAAAA 
CCTTTTGAAA GTAGTTCTTC TATTGGAGCG GAAAAACAAA GGAATCAAAA ACAGCCTGAA 
GAGGAGGTGG AGAACAGTAG GACACCATGG TTTTATGACC AAGAAGGTGA AGTAGAAAAG 
CCATTTCTCA AGACTGGATT TGCAGTGTCT GTAGAGAAAA CAACAAATAG TAATCGCAAA 
AATCAACTAG ATACCAGTAG GAGAAGACGC CAGTTTGATG AAGTGTCCTT AGAAAGCTTT 
AACAGTATGC TTGACCCAGT AGATCCAACA ACAGTAACTA AAACATTCAA GTCAAGAAAA 
GCATCTGCAC AGGCCAGCCT GGCATCTAAA GATAAAACTC CCAAATCAAA GAGTAAGAAG 
AGGCATTCTA CTCAGCTGAA AAGCAGAGTG AAAAATATCG GGTATGAAAG TGCCAGTGTG 
TCTAGCACAT GTGAACCTTG CAAAAGTAGA AACAGACATT CAGCCCAAAC TGAAGAGCCT 
GTTCAAGCAA AAGTACTCAG CAGAAAGAAT CATGAGCAGC TGGAAAAAGT CATAAGATGT 
AGTAGGTCTA CAGAAATATC TTCAGCACAT GCTAGGAGAA TACTACAGCA GTCTAACAGA 
AATGCATGCA ATGAAGCGCC AGAAACCGGG AGTGATTTTT CCATGTTCGA AGCTTTGCGG 
GATACCATTT ATTCTGAAGT AGCTACGTTG ATTTCTCAAA ATGAATCTCG TCCACATTTT 
CTTATTGAAC TCTTCCATGA GCTTCAGCTA CTTAATACAG ACTATTTGAG ACAGAGGGCC 
TTATATGCAT TGCAGGACAT AGTATCCAGG CATATTTCTG AGAACCATGA AAAAGAAGGA 
GAAAATATAA AGTCCGTGAA TTCTGGTACT TGGATAGCAT CCAACTCAGA ACTTACTCCC 
AGTGAAAGCC TTGCTACTAC TGATGATGAA ACTTTTGAGA AGAACTTTGA GAGAGAAACA 
CATAAAATAA GTGAGCAAAA TGATGCTGAT AATGCTAGTG TATCTTCAAC TTGTGAGCCT 
TTTGCAACAG ATGTTCTGGG CGACACTGTG ATTCACTTAG ATCAGGCATT AGCCAGAATG 
AGAGACTATA CGCATATGAA GATGGAGGCT GAAAGTAGTC CAGACGTCAG GTGCACCTGC 
AGGATCATTG AGGATGACGA TGGTGCTGCT GCCACAACCA CGATTAGTAA TAATTTAGAA 
GAAACTCCTG TTATTGAAAA TCATAGTTCA CAGCAACCCG TGAGTGAAGT TGCTACCGTC 
CCGTGTCCTA GAATTGATAC TCAGCAACTG GACCGGCAAA TTAAAGCAAT TATGAAAGAA 
GTCATTCCTT TTTTGAAGGA GCACATGGAT GAAGTATGTT CTTCTCAACT TCTAACCTCA 
GTAAGACGCA TGGTTTTGGT CCTTACTCAG CAAAACAATG AGAGCAAAGA GTTTGTAAAG 
TTCTTTCATA AGCAGCTTGG AAGTATATTA CAGGATTCAC TCGCAAAATT TGCTGGCAGA 
AAACTGAAGG ATTGTGGAGA AGATCTTCTT GTAGAGATAT CTGAAGTGTT ATTTAATGAA 
TTGGCTTTCT TTAAGCTCAT GCAAGATTTG GATAATAATA GTGTAACTGT TAAACAGAGA 
TGCAAAAGGA AAATAGAAGC TGCTGGAGTG ATACAGTCTT ATGCAAAAGA GGCCAAAAGG 
ATTCTTGAAG GAGATCACAG TTCACCTGCT GGAGAAATTG ATGATGAAGA CAAAGACAAG 
GATGAGACGG AAACAGTTAA GCAGACTCAA ACGTCTGAGG TTTATGATGG TGACGGCCCC 
AAAAATGTGA GATCTGATGT TTCTGATCAA GAGGATGATG AAGAAAGTGA AGAATGTCCA 
GTGTCTATTA ATTTGTCCAA AGCTGAAACT CAGGCTTTGA CTAATTACGG AAGTGGAGAA 
GATGAGAATG AAGATGAAGA AATAGAAGAA TTTGAAGAGG GACCTGTAGA TGTCCAGACG 
TCACTTCAGG CTAACACTGA AACTACAGAA GAAAATGAAC ACGATGATCA GGTTCCACAG 
CATGATTTTG AAAAATCATC AGAAAGCAAA AATGTCCCAT CAGAACAAGA ACCCACTACT 
AGTAAAGGTG ACCAGAATAG CACTCCTGTG AAGCCCTGTT ACCTCAGTAT CGTGGAAAAT 
GAGCAACCTT TAAATAGCCC TGTCCAGAAG GACACTCTCA CTACCATCGA CTCATCCAGT 
CAGCCTAACC CTTTGCCTTT ACCTTTAACT GAAATTGAAA CCTTGGTGCC TAGAGTCAAA 
GAAGTGAAAT CTGCTCAGGA GACTCCTGAA AGTTCTTTGG CTGGAAGTCC TGATACTGAA 
TCTCCAGTGT TAGTGAATGA CTATGAAGCA GAATCTGGTA ACATAAGTCA AAAATCTGAT 
GAAGAAGACT TTGTGAAAGT TGAAGATTTA CCACTTAAAC TGACAATATA TTCAGAGGCA 
GATCTAAGGA AGAAAATGGC CGAAGAAGAA CAGAAAAACC ACTTATCTGG TGAAATATGT 
GCAATGCAGA CTGAAGAATT AGCTGGGAAT TCTCAGACAC TAAAAGAACC TGAGCAAGTA 
TGTGCTCAGT CTGCCATCTT AAGATGGAAA CCCTTACCTG TGCCTTTTAA ACTGTTGGTG 
GTGTCATCAG GGAATTATTG TAGCAAAGTA AGTTTCTTTT CTAATAACTT TATATTAAAA 
TTGAAAGTTT TAAATGGAAG AGAACAATAT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( XM_006080137.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006080199.1 Bubalus bubalis pericentriolar material 1 (PCM1), transcript variant X4, mRNA.

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CloneID	OBu33064
Clone ID Related Accession (Same CDS sequence)	XM_006080137.1
Accession Version	XM_006080137.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6519bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	pericentriolar material 1 protein isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785878.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG GAGGAGGTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
AGCAATGAGA GTGTTGATGA CCGACTCAAC AATATGGATT GGGGCAGCCA GCAGAAGAAG 
GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA 
ACTAACGATA TTTCTCCGGA GTCATCACCG GGAGTTGGAA GACGAAGAAC AAAGACTTCA 
CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TCCCCGAGCA GGCAGAACTA 
GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGATTAATAC TAACAAGAGC 
AAAGATGCCA CTGTAAGTCC TCAAAAGAGA GAAATGATTG GATCAGCACA GTGTAAAGAG 
TTGTTTGCTT CTGCTTTAAG TAATGACCTT TTGCAAAACT GTCAAGTCTC TGAAGAAGAT 
GGAAGAGGAG AACCTGCAAT GGAGAGCAGC CAGATTGTAA GCAGGCTTGT TCAGATTCGT 
GAGTATATTA CTAAAGCTAG TTCCATGCGG GAAGACCTTG TAGAGAAAAA TGAAAGATCT 
GCTAATGTTG AGCGCCTCAC TCATCTAATA GATCACCTTA AAGAACAAGA AAAGTCATAT 
ATGAAATTTC TCCAGAAGAT TCTTGCTAGA GAAAATGAGG AGGAGGATGT TCGGACTATA 
GATTCAGCTG TGGGATCTGG TTCTGTAGCT GAGAGCACAT CGCTAAACAT AGATGTGCGG 
TCTGAGGCTT CAGATGCCAC GGAGGCATCT TTTAGTCTGA GAATTCGGCC GTGCGTTGAG 
GACAAACTAG GGAATTCAGC TTCACAAGCA CAGGTTTCAG ACACTGACGT TACTACAAGT 
CCAAAAGGGA AAGGTGACAG ACCTCCTCAG CATGACAGGG AGCTGAGGCC TAATAGGAAA 
TGTAGCCGAA AACGTGGATT TCCCTCAAAG GCCAGAGATC CTCAGCAGGA GCCTATGGAA 
GAGATAGAAA ATTTGAAGAA ACAACATGAT TTATTAAAAA GGATGTTACA GCAGCAGGAG 
CAGCTGAGAG CTCTACAAGG ACGACAAGCT GCTCTGCTTG CCCTGCAGCA TAAAGCTGAG 
CAGGCTATAG CTGTGATGGA TGATTCTGAA AAGGTTGCGG AGACAACTCC TGGCCATCAT 
ACTGTACCAG GCAGACAGCC GGCTCATTCA CCTTTGCATC AGAGAGTACC CATAAGAGAA 
ACTACAGGTA GCCTATCTGG AGTCAGTATC ACCTCTGAAC TAAACGAAGA ATTGAATGAT 
TTAATTCAGC ATTTTCATAA TCAGCTTCGT GATTCTCAGC CTCCAGCTGT TCCAGACAAT 
AGAAGACAAG CAGAAAGTCT TTCATTAACT AGGGAGATTT CTCAGAGCAG AAATCCTTCA 
GTTTCAGAGC ATTTACCTGA TGAGAAAGTC CAACTTTTTA GCAAGATGAG AGTGTTACAA 
GAAAAGAAAC AAAAAATGGA CAAACTTCTT GGAGAACTTC ATACACTTCG AGATCAGCAT 
TTGAACAATT CATCATTTGT GCCTTCTTCA GCCTCTCCAC AAAGGAGTGT GGATCAGAGA 
AGTACAACCA CAGCTCCTTC TGCTCCTGTA GTCTTAACAC CAGTGGTCAA TGGAGAATCC 
AATAACCTCA CGCCATCTGT TCCTTATCCT GCTGCTTCTC TAGTTTCTCA GAATCAGAAT 
GAAAATGAAG GCCACCTAAA TCCAACTGAA AAACTCCAGA AGTTAAATGA AGTTCGAAAG 
AGGTTGAATG AACTAAGAGA ATTAGTTCAT TATTATGAGC AAACATCAGA TATGATGACA 
GATGCTGTGA ATGAAAACAC AAAAGAAGAA GAAACTGAAG AATCAGAATA TGATTCGGAG 
CATGAAAACT CGGAACCTGT TACTAACATT CGAAATCCAC AAGTGGCTGC CACCTGGAAC 
GAAGTAAATA GTAATTCTAA TGCACAGTGT GTTTCCAACA ATAGAGATGG GCGAGCAGTT 
AATTCTAACT GTGAAATTAA CAACAGGTCT GCTGCCAACA TAAGGGCTCT AAACATGCCT 
CCTCTTTCAG CAGATTGTCG ATACAATAGA GAAGGAGAAC AGGAGATGCC TGTTGCACAA 
GGTCATGAGG AGGAGGAGGA GGAGGAGGAG GAAGCAGAAG ATGAGGCCCT CAGTGGAGCT 
TCACTGTCTA GTCACAGCAG CAGCCTGATC ATTGAGACTC CTGAAGATGC TGAATTTGAA 
CATAAGATCC AGAAGCTTAT GGCTGCAAAA CTGAAACTTA GACACTTACA GAACCTTGTA 
GCCATGGTGC AGGACGATGA TGCAGCCAAT CAAGGAGTTA CTTCTGTGGA GGATTTCTTC 
CCAGCAGAAG ACACCAAACA AAATGCAAAT AACACTAGAG GAAATGCCAA TAAAACACAG 
AAAGATGCTG GAGTAAATGA AAAGGCAAGG GAGAAATTTT ATGAGGCTAA ACTACAGCAG 
CAACAGAGAG AACTCAAACA ATTGCAGGAA GAAAGAAAGA AACTGATTGA GATTCAAGAG 
AAAATTCAAG AATTGCAAAA GGCATGTCCT AACCTACAGC TGTCAGCTGC TAGTGTGGGT 
AATTGTCCCA CAAAAACACA TATGCCAGCT GTTACTTCAT CCCCAACCGT TAATGAAAAT 
GAAGCCAGTA CAAACAAATC TGGTTTTGAG CCTCATGATT CTTCAGTGGT AGATAATGAG 
GTATGGTCAG ATATGCGAAG ACATGAAATG TTAAGGGAGG AGCTACGACA GAGAAGAAAG 
CAGCTGGAAG CTCTGATGGC TGAACATCAG AGGAGGCAAG GTCTGGCTGA AACTGCATCT 
CCAGTGGCTG TTTCACTGAG AAGTGATGGA TCTGAGAACC TGTGTACACC TCAGCAAAGC 
AGAACAGAAA AAACAATGGC AACTTGGGGA GGTTCTACCC AGTGTGCCCT AGATGAAGAA 
GGAGATGAAG ATGGTTACCT TTCTGAAGGA ATTATTCGGA CAGACGAAGA GGAGGAAGAG 
GAGGAGCAAG ATGCCAGTTC CAATGATAAC TTTCATATGT ATCCTCCAAA CAGTGCAAAT 
CATAATTCTT ACAATGTAAA GGAAACTAAA AATAGGTGGA AGAACAATCG CCCTTTTTCA 
GCAGATGGAA ATTATCGTCC TTTAGCCAAG ACAAGACAGC AGAATATCAG CATGCAGCGT 
CAGGAAAACC TTCGCTGGGT GTCCGAACTC TCTTATGTAG AAGAGAAAGA ACAATGGCAA 
GAACAAATTA ATCAGCTAAA GAAACAACTT GATTTCAGTG TCAATATTTG TCAGACTTTA 
ATGCAAGACC AGCAGACTCT GTCTTGTCTG CTACAAACTC TTCTCACGAG TCCTTATAGT 
GTTATGCCCA GCAGTGTTGC ATCCCCGCAA GTGCACCTTA TAATGCACCA GTTGAATCAG 
TGCTATACCC AGCTAACGTG GCAGCAGAAC AATGTCCAGA GGTTGAAACA AATGCTCAGT 
GAACTCATGC GCCAACAAAA TCAGCATCCA GAAAAACCTG CAAGCAAGGA AGGAGGCAGT 
GGTACATCAC ACCCTCCTTC TCCCAGTTTA TTTTGTCCTT TCAACTTTCC AACTCAGCCT 
GTAAATCTGT TTAACCTACC GGGGTTTACT AATTTTTCGT CATTTGCACC AAGTATGAAT 
TTCAGCCCTT TATTTCCTTC TAATTTTGGA GATTTTTCTC AAAATATTTC TACACCCACT 
GAACAGCAGC AACCGTTAGC CCAGAATCCT TCAGGGAAAA CGGAATACAT GGCTTTTCCA 
AAACCTTTTG AAAGTAGTTC TTCTATTGGA GCGGAAAAAC AAAGGAATCA AAAACAGCCT 
GAAGAGGAGG TGGAGAACAG TAGGACACCA TGGTTTTATG ACCAAGAAGG TGAAGTAGAA 
AAGCCATTTC TCAAGACTGG ATTTGCAGTG TCTGTAGAGA AAACAACAAA TAGTAATCGC 
AAAAATCAAC TAGATACCAG TAGGAGAAGA CGCCAGTTTG ATGAAGTGTC CTTAGAAAGC 
TTTAACAGTA TGCTTGACCC AGTAGATCCA ACAACAGTAA CTAAAACATT CAAGTCAAGA 
AAAGCATCTG CACAGGCCAG CCTGGCATCT AAAGATAAAA CTCCCAAATC AAAGAGTAAG 
AAGAGGCATT CTACTCAGCT GAAAAGCAGA GTGAAAAATA TCGGGTATGA AAGTGCCAGT 
GTGTCTAGCA CATGTGAACC TTGCAAAAGT AGAAACAGAC ATTCAGCCCA AACTGAAGAG 
CCTGTTCAAG CAAAAGTACT CAGCAGAAAG AATCATGAGC AGCTGGAAAA AGTCATAAGA 
TGTAGTAGGT CTACAGAAAT ATCTTCAGAA ACCGGGAGTG ATTTTTCCAT GTTCGAAGCT 
TTGCGGGATA CCATTTATTC TGAAGTAGCT ACGTTGATTT CTCAAAATGA ATCTCGTCCA 
CATTTTCTTA TTGAACTCTT CCATGAGCTT CAGCTACTTA ATACAGACTA TTTGAGACAG 
AGGGCCTTAT ATGCATTGCA GGACATAGTA TCCAGGCATA TTTCTGAGAA CCATGAAAAA 
GAAGGAGAAA ATATAAAGTC CGTGAATTCT GGTACTTGGA TAGCATCCAA CTCAGAACTT 
ACTCCCAGTG AAAGCCTTGC TACTACTGAT GATGAAACTT TTGAGAAGAA CTTTGAGAGA 
GAAACACATA AAATAAGTGA GCAAAATGAT GCTGATAATG CTAGTGTATC TTCAACTTGT 
GAGCCTTTTG CAACAGATGT TCTGGGCGAC ACTGTGATTC ACTTAGATCA GGCATTAGCC 
AGAATGAGAG ACTATACGCA TATGAAGATG GAGGCTGAAA GTAGTCCAGA CGTCAGGTGC 
ACCTGCAGGA TCATTGAGGA TGACGATGGT GCTGCTGCCA CAACCACGAT TAGTAATAAT 
TTAGAAGAAA CTCCTGTTAT TGAAAATCAT AGTTCACAGC AACCCGTGAG TGAAGTTGCT 
ACCGTCCCGT GTCCTAGAAT TGATACTCAG CAACTGGACC GGCAAATTAA AGCAATTATG 
AAAGAAGTCA TTCCTTTTTT GAAGGAGCAC ATGGATGAAG TATGTTCTTC TCAACTTCTA 
ACCTCAGTAA GACGCATGGT TTTGGTCCTT ACTCAGCAAA ACAATGAGAG CAAAGAGTTT 
GTAAAGTTCT TTCATAAGCA GCTTGGAAGT ATATTACAGG ATTCACTCGC AAAATTTGCT 
GGCAGAAAAC TGAAGGATTG TGGAGAAGAT CTTCTTGTAG AGATATCTGA AGTGTTATTT 
AATGAATTGG CTTTCTTTAA GCTCATGCAA GATTTGGATA ATAATAGTGT AACTGTTAAA 
CAGAGATGCA AAAGGAAAAT AGAAGCTGCT GGAGTGATAC AGTCTTATGC AAAAGAGGCC 
AAAAGGATTC TTGAAGGAGA TCACAGTTCA CCTGCTGGAG AAATTGATGA TGAAGACAAA 
GACAAGGATG AGACGGAAAC AGTTAAGCAG ACTCAAACGT CTGAGGTTTA TGATGGTGAC 
GGCCCCAAAA ATGTGAGATC TGATGTTTCT GATCAAGAGG ATGATGAAGA AAGTGAAGAA 
TGTCCAGTGT CTATTAATTT GTCCAAAGCT GAAACTCAGG CTTTGACTAA TTACGGAAGT 
GGAGAAGATG AGAATGAAGA TGAAGAAATA GAAGAATTTG AAGAGGGACC TGTAGATGTC 
CAGACGTCAC TTCAGGCTAA CACTGAAACT ACAGAAGAAA ATGAACACGA TGATCAGGTT 
CCACAGCATG ATTTTGAAAA ATCATCAGAA AGCAAAAATG TCCCATCAGA ACAAGAACCC 
ACTACTAGTA AAGGTGACCA GAATAGCACT CCTGTGAAGC CCTGTTACCT CAGTATCGTG 
GAAAATGAGC AACCTTTAAA TAGCCCTGTC CAGAAGGACA CTCTCACTAC CATCGACTCA 
TCCAGTCAGC CTAACCCTTT GCCTTTACCT TTAACTGAAA TTGAAACCTT GGTGCCTAGA 
GTCAAAGAAG TGAAATCTGC TCAGGAGACT CCTGAAAGTT CTTTGGCTGG AAGTCCTGAT 
ACTGAATCTC CAGTGTTAGT GAATGACTAT GAAGCAGAAT CTGGTAACAT AAGTCAAAAA 
TCTGATGAAG AAGACTTTGT GAAAGTTGAA GATTTACCAC TTAAACTGAC AATATATTCA 
GAGGCAGATC TAAGGAAGAA AATGGCCGAA GAAGAACAGA AAAACCACTT ATCTGGTGAA 
ATATGTGCAA TGCAGACTGA AGAATTAGCT GGGAATTCTC AGACACTAAA AGAACCTGAG 
CAAGTATGTG CTCAGTCTGC CATCTTAAGA TGGAAACCCT TACCTGTGCC TTTTAAACTG 
TTGGTGGTGT CATCAGGGAA TTATTGTAGC AAAGTAAGTT TCTTTTCTAA TAACTTTATA 
TTAAAATTGA AAGTTTTAAA TGGAAGAGAA CAATATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006080199.1
CDS	113..6631
Translation	MATGGGPFEEGVNDQDLPNWSNESVDDRLNNMDWGSQQKKANRSSEKNKRKFGVESDKRVTNDISPESSPGVGRRRTKTSLTFPHSRYMTQMSVPEQAELEKLKQRINFSDLDQINTNKSKDATVSPQKREMIGSAQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIREYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLQKILARENEEEDVRTIDSAVGSGSVAESTSLNIDVRSEASDATEASFSLRIRPCVEDKLGNSASQAQVSDTDVTTSPKGKGDRPPQHDRELRPNRKCSRKRGFPSKARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSEKVAETTPGHHTVPGRQPAHSPLHQRVPIRETTGSLSGVSITSELNEELNDLIQHFHNQLRDSQPPAVPDNRRQAESLSLTREISQSRNPSVSEHLPDEKVQLFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSFVPSSASPQRSVDQRSTTTAPSAPVVLTPVVNGESNNLTPSVPYPAASLVSQNQNENEGHLNPTEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENTKEEETEESEYDSEHENSEPVTNIRNPQVAATWNEVNSNSNAQCVSNNRDGRAVNSNCEINNRSAANIRALNMPPLSADCRYNREGEQEMPVAQGHEEEEEEEEEAEDEALSGASLSSHSSSLIIETPEDAEFEHKIQKLMAAKLKLRHLQNLVAMVQDDDAANQGVTSVEDFFPAEDTKQNANNTRGNANKTQKDAGVNEKAREKFYEAKLQQQQRELKQLQEERKKLIEIQEKIQELQKACPNLQLSAASVGNCPTKTHMPAVTSSPTVNENEASTNKSGFEPHDSSVVDNEVWSDMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETASPVAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGIIRTDEEEEEEEQDASSNDNFHMYPPNSANHNSYNVKETKNRWKNNRPFSADGNYRPLAKTRQQNISMQRQENLRWVSELSYVEEKEQWQEQINQLKKQLDFSVNICQTLMQDQQTLSCLLQTLLTSPYSVMPSSVASPQVHLIMHQLNQCYTQLTWQQNNVQRLKQMLSELMRQQNQHPEKPASKEGGSGTSHPPSPSLFCPFNFPTQPVNLFNLPGFTNFSSFAPSMNFSPLFPSNFGDFSQNISTPTEQQQPLAQNPSGKTEYMAFPKPFESSSSIGAEKQRNQKQPEEEVENSRTPWFYDQEGEVEKPFLKTGFAVSVEKTTNSNRKNQLDTSRRRRQFDEVSLESFNSMLDPVDPTTVTKTFKSRKASAQASLASKDKTPKSKSKKRHSTQLKSRVKNIGYESASVSSTCEPCKSRNRHSAQTEEPVQAKVLSRKNHEQLEKVIRCSRSTEISSETGSDFSMFEALRDTIYSEVATLISQNESRPHFLIELFHELQLLNTDYLRQRALYALQDIVSRHISENHEKEGENIKSVNSGTWIASNSELTPSESLATTDDETFEKNFERETHKISEQNDADNASVSSTCEPFATDVLGDTVIHLDQALARMRDYTHMKMEAESSPDVRCTCRIIEDDDGAAATTTISNNLEETPVIENHSSQQPVSEVATVPCPRIDTQQLDRQIKAIMKEVIPFLKEHMDEVCSSQLLTSVRRMVLVLTQQNNESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSVTVKQRCKRKIEAAGVIQSYAKEAKRILEGDHSSPAGEIDDEDKDKDETETVKQTQTSEVYDGDGPKNVRSDVSDQEDDEESEECPVSINLSKAETQALTNYGSGEDENEDEEIEEFEEGPVDVQTSLQANTETTEENEHDDQVPQHDFEKSSESKNVPSEQEPTTSKGDQNSTPVKPCYLSIVENEQPLNSPVQKDTLTTIDSSSQPNPLPLPLTEIETLVPRVKEVKSAQETPESSLAGSPDTESPVLVNDYEAESGNISQKSDEEDFVKVEDLPLKLTIYSEADLRKKMAEEEQKNHLSGEICAMQTEELAGNSQTLKEPEQVCAQSAILRWKPLPVPFKLLVVSSGNYCSKVSFFSNNFILKLKVLNGREQY

Target ORF information:

RefSeq Version	XM_006080137.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis pericentriolar material 1 (PCM1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006080137.1

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
AGCAATGAGA GTGTTGATGA CCGACTCAAC AATATGGATT GGGGCAGCCA GCAGAAGAAG 
GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA 
ACTAACGATA TTTCTCCGGA GTCATCACCG GGAGTTGGAA GACGAAGAAC AAAGACTTCA 
CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TCCCCGAGCA GGCAGAACTA 
GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGATTAATAC TAACAAGAGC 
AAAGATGCCA CTGTAAGTCC TCAAAAGAGA GAAATGATTG GATCAGCACA GTGTAAAGAG 
TTGTTTGCTT CTGCTTTAAG TAATGACCTT TTGCAAAACT GTCAAGTCTC TGAAGAAGAT 
GGAAGAGGAG AACCTGCAAT GGAGAGCAGC CAGATTGTAA GCAGGCTTGT TCAGATTCGT 
GAGTATATTA CTAAAGCTAG TTCCATGCGG GAAGACCTTG TAGAGAAAAA TGAAAGATCT 
GCTAATGTTG AGCGCCTCAC TCATCTAATA GATCACCTTA AAGAACAAGA AAAGTCATAT 
ATGAAATTTC TCCAGAAGAT TCTTGCTAGA GAAAATGAGG AGGAGGATGT TCGGACTATA 
GATTCAGCTG TGGGATCTGG TTCTGTAGCT GAGAGCACAT CGCTAAACAT AGATGTGCGG 
TCTGAGGCTT CAGATGCCAC GGAGGCATCT TTTAGTCTGA GAATTCGGCC GTGCGTTGAG 
GACAAACTAG GGAATTCAGC TTCACAAGCA CAGGTTTCAG ACACTGACGT TACTACAAGT 
CCAAAAGGGA AAGGTGACAG ACCTCCTCAG CATGACAGGG AGCTGAGGCC TAATAGGAAA 
TGTAGCCGAA AACGTGGATT TCCCTCAAAG GCCAGAGATC CTCAGCAGGA GCCTATGGAA 
GAGATAGAAA ATTTGAAGAA ACAACATGAT TTATTAAAAA GGATGTTACA GCAGCAGGAG 
CAGCTGAGAG CTCTACAAGG ACGACAAGCT GCTCTGCTTG CCCTGCAGCA TAAAGCTGAG 
CAGGCTATAG CTGTGATGGA TGATTCTGAA AAGGTTGCGG AGACAACTCC TGGCCATCAT 
ACTGTACCAG GCAGACAGCC GGCTCATTCA CCTTTGCATC AGAGAGTACC CATAAGAGAA 
ACTACAGGTA GCCTATCTGG AGTCAGTATC ACCTCTGAAC TAAACGAAGA ATTGAATGAT 
TTAATTCAGC ATTTTCATAA TCAGCTTCGT GATTCTCAGC CTCCAGCTGT TCCAGACAAT 
AGAAGACAAG CAGAAAGTCT TTCATTAACT AGGGAGATTT CTCAGAGCAG AAATCCTTCA 
GTTTCAGAGC ATTTACCTGA TGAGAAAGTC CAACTTTTTA GCAAGATGAG AGTGTTACAA 
GAAAAGAAAC AAAAAATGGA CAAACTTCTT GGAGAACTTC ATACACTTCG AGATCAGCAT 
TTGAACAATT CATCATTTGT GCCTTCTTCA GCCTCTCCAC AAAGGAGTGT GGATCAGAGA 
AGTACAACCA CAGCTCCTTC TGCTCCTGTA GTCTTAACAC CAGTGGTCAA TGGAGAATCC 
AATAACCTCA CGCCATCTGT TCCTTATCCT GCTGCTTCTC TAGTTTCTCA GAATCAGAAT 
GAAAATGAAG GCCACCTAAA TCCAACTGAA AAACTCCAGA AGTTAAATGA AGTTCGAAAG 
AGGTTGAATG AACTAAGAGA ATTAGTTCAT TATTATGAGC AAACATCAGA TATGATGACA 
GATGCTGTGA ATGAAAACAC AAAAGAAGAA GAAACTGAAG AATCAGAATA TGATTCGGAG 
CATGAAAACT CGGAACCTGT TACTAACATT CGAAATCCAC AAGTGGCTGC CACCTGGAAC 
GAAGTAAATA GTAATTCTAA TGCACAGTGT GTTTCCAACA ATAGAGATGG GCGAGCAGTT 
AATTCTAACT GTGAAATTAA CAACAGGTCT GCTGCCAACA TAAGGGCTCT AAACATGCCT 
CCTCTTTCAG CAGATTGTCG ATACAATAGA GAAGGAGAAC AGGAGATGCC TGTTGCACAA 
GGTCATGAGG AGGAGGAGGA GGAGGAGGAG GAAGCAGAAG ATGAGGCCCT CAGTGGAGCT 
TCACTGTCTA GTCACAGCAG CAGCCTGATC ATTGAGACTC CTGAAGATGC TGAATTTGAA 
CATAAGATCC AGAAGCTTAT GGCTGCAAAA CTGAAACTTA GACACTTACA GAACCTTGTA 
GCCATGGTGC AGGACGATGA TGCAGCCAAT CAAGGAGTTA CTTCTGTGGA GGATTTCTTC 
CCAGCAGAAG ACACCAAACA AAATGCAAAT AACACTAGAG GAAATGCCAA TAAAACACAG 
AAAGATGCTG GAGTAAATGA AAAGGCAAGG GAGAAATTTT ATGAGGCTAA ACTACAGCAG 
CAACAGAGAG AACTCAAACA ATTGCAGGAA GAAAGAAAGA AACTGATTGA GATTCAAGAG 
AAAATTCAAG AATTGCAAAA GGCATGTCCT AACCTACAGC TGTCAGCTGC TAGTGTGGGT 
AATTGTCCCA CAAAAACACA TATGCCAGCT GTTACTTCAT CCCCAACCGT TAATGAAAAT 
GAAGCCAGTA CAAACAAATC TGGTTTTGAG CCTCATGATT CTTCAGTGGT AGATAATGAG 
GTATGGTCAG ATATGCGAAG ACATGAAATG TTAAGGGAGG AGCTACGACA GAGAAGAAAG 
CAGCTGGAAG CTCTGATGGC TGAACATCAG AGGAGGCAAG GTCTGGCTGA AACTGCATCT 
CCAGTGGCTG TTTCACTGAG AAGTGATGGA TCTGAGAACC TGTGTACACC TCAGCAAAGC 
AGAACAGAAA AAACAATGGC AACTTGGGGA GGTTCTACCC AGTGTGCCCT AGATGAAGAA 
GGAGATGAAG ATGGTTACCT TTCTGAAGGA ATTATTCGGA CAGACGAAGA GGAGGAAGAG 
GAGGAGCAAG ATGCCAGTTC CAATGATAAC TTTCATATGT ATCCTCCAAA CAGTGCAAAT 
CATAATTCTT ACAATGTAAA GGAAACTAAA AATAGGTGGA AGAACAATCG CCCTTTTTCA 
GCAGATGGAA ATTATCGTCC TTTAGCCAAG ACAAGACAGC AGAATATCAG CATGCAGCGT 
CAGGAAAACC TTCGCTGGGT GTCCGAACTC TCTTATGTAG AAGAGAAAGA ACAATGGCAA 
GAACAAATTA ATCAGCTAAA GAAACAACTT GATTTCAGTG TCAATATTTG TCAGACTTTA 
ATGCAAGACC AGCAGACTCT GTCTTGTCTG CTACAAACTC TTCTCACGAG TCCTTATAGT 
GTTATGCCCA GCAGTGTTGC ATCCCCGCAA GTGCACCTTA TAATGCACCA GTTGAATCAG 
TGCTATACCC AGCTAACGTG GCAGCAGAAC AATGTCCAGA GGTTGAAACA AATGCTCAGT 
GAACTCATGC GCCAACAAAA TCAGCATCCA GAAAAACCTG CAAGCAAGGA AGGAGGCAGT 
GGTACATCAC ACCCTCCTTC TCCCAGTTTA TTTTGTCCTT TCAACTTTCC AACTCAGCCT 
GTAAATCTGT TTAACCTACC GGGGTTTACT AATTTTTCGT CATTTGCACC AAGTATGAAT 
TTCAGCCCTT TATTTCCTTC TAATTTTGGA GATTTTTCTC AAAATATTTC TACACCCACT 
GAACAGCAGC AACCGTTAGC CCAGAATCCT TCAGGGAAAA CGGAATACAT GGCTTTTCCA 
AAACCTTTTG AAAGTAGTTC TTCTATTGGA GCGGAAAAAC AAAGGAATCA AAAACAGCCT 
GAAGAGGAGG TGGAGAACAG TAGGACACCA TGGTTTTATG ACCAAGAAGG TGAAGTAGAA 
AAGCCATTTC TCAAGACTGG ATTTGCAGTG TCTGTAGAGA AAACAACAAA TAGTAATCGC 
AAAAATCAAC TAGATACCAG TAGGAGAAGA CGCCAGTTTG ATGAAGTGTC CTTAGAAAGC 
TTTAACAGTA TGCTTGACCC AGTAGATCCA ACAACAGTAA CTAAAACATT CAAGTCAAGA 
AAAGCATCTG CACAGGCCAG CCTGGCATCT AAAGATAAAA CTCCCAAATC AAAGAGTAAG 
AAGAGGCATT CTACTCAGCT GAAAAGCAGA GTGAAAAATA TCGGGTATGA AAGTGCCAGT 
GTGTCTAGCA CATGTGAACC TTGCAAAAGT AGAAACAGAC ATTCAGCCCA AACTGAAGAG 
CCTGTTCAAG CAAAAGTACT CAGCAGAAAG AATCATGAGC AGCTGGAAAA AGTCATAAGA 
TGTAGTAGGT CTACAGAAAT ATCTTCAGAA ACCGGGAGTG ATTTTTCCAT GTTCGAAGCT 
TTGCGGGATA CCATTTATTC TGAAGTAGCT ACGTTGATTT CTCAAAATGA ATCTCGTCCA 
CATTTTCTTA TTGAACTCTT CCATGAGCTT CAGCTACTTA ATACAGACTA TTTGAGACAG 
AGGGCCTTAT ATGCATTGCA GGACATAGTA TCCAGGCATA TTTCTGAGAA CCATGAAAAA 
GAAGGAGAAA ATATAAAGTC CGTGAATTCT GGTACTTGGA TAGCATCCAA CTCAGAACTT 
ACTCCCAGTG AAAGCCTTGC TACTACTGAT GATGAAACTT TTGAGAAGAA CTTTGAGAGA 
GAAACACATA AAATAAGTGA GCAAAATGAT GCTGATAATG CTAGTGTATC TTCAACTTGT 
GAGCCTTTTG CAACAGATGT TCTGGGCGAC ACTGTGATTC ACTTAGATCA GGCATTAGCC 
AGAATGAGAG ACTATACGCA TATGAAGATG GAGGCTGAAA GTAGTCCAGA CGTCAGGTGC 
ACCTGCAGGA TCATTGAGGA TGACGATGGT GCTGCTGCCA CAACCACGAT TAGTAATAAT 
TTAGAAGAAA CTCCTGTTAT TGAAAATCAT AGTTCACAGC AACCCGTGAG TGAAGTTGCT 
ACCGTCCCGT GTCCTAGAAT TGATACTCAG CAACTGGACC GGCAAATTAA AGCAATTATG 
AAAGAAGTCA TTCCTTTTTT GAAGGAGCAC ATGGATGAAG TATGTTCTTC TCAACTTCTA 
ACCTCAGTAA GACGCATGGT TTTGGTCCTT ACTCAGCAAA ACAATGAGAG CAAAGAGTTT 
GTAAAGTTCT TTCATAAGCA GCTTGGAAGT ATATTACAGG ATTCACTCGC AAAATTTGCT 
GGCAGAAAAC TGAAGGATTG TGGAGAAGAT CTTCTTGTAG AGATATCTGA AGTGTTATTT 
AATGAATTGG CTTTCTTTAA GCTCATGCAA GATTTGGATA ATAATAGTGT AACTGTTAAA 
CAGAGATGCA AAAGGAAAAT AGAAGCTGCT GGAGTGATAC AGTCTTATGC AAAAGAGGCC 
AAAAGGATTC TTGAAGGAGA TCACAGTTCA CCTGCTGGAG AAATTGATGA TGAAGACAAA 
GACAAGGATG AGACGGAAAC AGTTAAGCAG ACTCAAACGT CTGAGGTTTA TGATGGTGAC 
GGCCCCAAAA ATGTGAGATC TGATGTTTCT GATCAAGAGG ATGATGAAGA AAGTGAAGAA 
TGTCCAGTGT CTATTAATTT GTCCAAAGCT GAAACTCAGG CTTTGACTAA TTACGGAAGT 
GGAGAAGATG AGAATGAAGA TGAAGAAATA GAAGAATTTG AAGAGGGACC TGTAGATGTC 
CAGACGTCAC TTCAGGCTAA CACTGAAACT ACAGAAGAAA ATGAACACGA TGATCAGGTT 
CCACAGCATG ATTTTGAAAA ATCATCAGAA AGCAAAAATG TCCCATCAGA ACAAGAACCC 
ACTACTAGTA AAGGTGACCA GAATAGCACT CCTGTGAAGC CCTGTTACCT CAGTATCGTG 
GAAAATGAGC AACCTTTAAA TAGCCCTGTC CAGAAGGACA CTCTCACTAC CATCGACTCA 
TCCAGTCAGC CTAACCCTTT GCCTTTACCT TTAACTGAAA TTGAAACCTT GGTGCCTAGA 
GTCAAAGAAG TGAAATCTGC TCAGGAGACT CCTGAAAGTT CTTTGGCTGG AAGTCCTGAT 
ACTGAATCTC CAGTGTTAGT GAATGACTAT GAAGCAGAAT CTGGTAACAT AAGTCAAAAA 
TCTGATGAAG AAGACTTTGT GAAAGTTGAA GATTTACCAC TTAAACTGAC AATATATTCA 
GAGGCAGATC TAAGGAAGAA AATGGCCGAA GAAGAACAGA AAAACCACTT ATCTGGTGAA 
ATATGTGCAA TGCAGACTGA AGAATTAGCT GGGAATTCTC AGACACTAAA AGAACCTGAG 
CAAGTATGTG CTCAGTCTGC CATCTTAAGA TGGAAACCCT TACCTGTGCC TTTTAAACTG 
TTGGTGGTGT CATCAGGGAA TTATTGTAGC AAAGTAAGTT TCTTTTCTAA TAACTTTATA 
TTAAAATTGA AAGTTTTAAA TGGAAGAGAA CAATATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( XM_006080138.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006080200.1 Bubalus bubalis pericentriolar material 1 (PCM1), transcript variant X5, mRNA.

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CloneID	OBu33065
Clone ID Related Accession (Same CDS sequence)	XM_006080138.1
Accession Version	XM_006080138.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6513bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	pericentriolar material 1 protein isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785878.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG GAGGAGGTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
AGCAATGAGA GTGTTGATGA CCGACTCAAC AATATGGATT GGGGCAGCCA GCAGAAGAAG 
GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA 
ACTAACGATA TTTCTCCGGA GTCATCACCG GGAGTTGGAA GACGAAGAAC AAAGACTTCA 
CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TCCCCGAGCA GGCAGAACTA 
GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGATTAATAC TAACAAGAGC 
AAAGATGCCA CTGTAAGTCC TCAAAAGAGA GAAATGATTG GATCAGCACA GTGTAAAGAG 
TTGTTTGCTT CTGCTTTAAG TAATGACCTT TTGCAAAACT GTCAAGTCTC TGAAGAAGAT 
GGAAGAGGAG AACCTGCAAT GGAGAGCAGC CAGATTGTAA GCAGGCTTGT TCAGATTCGT 
GAGTATATTA CTAAAGCTAG TTCCATGCGG GAAGACCTTG TAGAGAAAAA TGAAAGATCT 
GCTAATGTTG AGCGCCTCAC TCATCTAATA GATCACCTTA AAGAACAAGA AAAGTCATAT 
ATGAAATTTC TCCAGAAGAT TCTTGCTAGA GAAAATGAGG AGGAGGATGT TCGGACTATA 
GATTCAGCTG TGGGATCTGG TTCTGTAGCT GAGAGCACAT CGCTAAACAT AGATGTGCGG 
TCTGAGGCTT CAGATGCCAC GGAGGCATCT TTTAGTCTGA GAATTCGGCC GTGCGTTGAG 
GACAAACTAG GGAATTCAGC TTCACAAGCA CAGGTTTCAG ACACTGACGT TACTACAAGT 
CCAAAAGGGA AAGGTGACAG ACCTCCTCAG CATGACAGGG AGCTGAGGCC TAATAGGAAA 
TGTAGCCGAA AACGTGGATT TCCCTCAAAG GCCAGAGATC CTCAGCAGGA GCCTATGGAA 
GAGATAGAAA ATTTGAAGAA ACAACATGAT TTATTAAAAA GGATGTTACA GCAGCAGGAG 
CAGCTGAGAG CTCTACAAGG ACGACAAGCT GCTCTGCTTG CCCTGCAGCA TAAAGCTGAG 
CAGGCTATAG CTGTGATGGA TGATTCTGAA AAGGTTGCGG AGACAACTCC TGGCCATCAT 
ACTGTACCAG GCAGACAGCC GGCTCATTCA CCTTTGCATC AGAGAGTACC CATAAGAGAA 
ACTACAGGTA GCCTATCTGG AGTCAGTATC ACCTCTGAAC TAAACGAAGA ATTGAATGAT 
TTAATTCAGC ATTTTCATAA TCAGCTTCGT GATTCTCAGC CTCCAGCTGT TCCAGACAAT 
AGAAGACAAG CAGAAAGTCT TTCATTAACT AGGGAGATTT CTCAGAGCAG AAATCCTTCA 
GTTTCAGAGC ATTTACCTGA TGAGAAAGTC CAACTTTTTA GCAAGATGAG AGTGTTACAA 
GAAAAGAAAC AAAAAATGGA CAAACTTCTT GGAGAACTTC ATACACTTCG AGATCAGCAT 
TTGAACAATT CATCATTTGT GCCTTCTTCA GCCTCTCCAC AAAGGAGTGT GGATCAGAGA 
AGTACAACCA CAGCTCCTTC TGCTCCTGTA GTCTTAACAC CAGTGGTCAA TGGAGAATCC 
AATAACCTCA CGCCATCTGT TCCTTATCCT GCTGCTTCTC TAGTTTCTCA GAATCAGAAT 
GAAAATGAAG GCCACCTAAA TCCAACTGAA AAACTCCAGA AGTTAAATGA AGTTCGAAAG 
AGGTTGAATG AACTAAGAGA ATTAGTTCAT TATTATGAGC AAACATCAGA TATGATGACA 
GATGCTGTGA ATGAAAACAC AAAAGAAGAA GAAACTGAAG AATCAGAATA TGATTCGGAG 
CATGAAAACT CGGAACCTGT TACTAACATT CGAAATCCAC AAGTGGCTGC CACCTGGAAC 
GAAGTAAATA GTAATTCTAA TGCACAGTGT GTTTCCAACA ATAGAGATGG GCGAGCAGTT 
AATTCTAACT GTGAAATTAA CAACAGGTCT GCTGCCAACA TAAGGGCTCT AAACATGCCT 
CCTCTTTCAG CAGATTGTCG ATACAATAGA GAAGGAGAAC AGGAGATGCC TGTTGCACAA 
GGTCATGAGG AGGAGGAGGA GGAGGAGGAG GAAGCAGAAG ATGAGGCCCT CAGTGGAGCT 
TCACTGTCTA GTCACAGCAG CAGCCTGATC ATTGAGACTC CTGAAGATGC TGAATTTGAA 
CATAAGATCC AGAAGCTTAT GGCTGCAAAA CTGAAACTTA GACACTTACA GAACCTTGTA 
GCCATGGTGC AGGACGATGA TGCAGCCAAT CAAGGAGTTA CTTCTGTGGA GGATTTCTTC 
CCAGCAGAAG ACACCAAACA AAATGCAAAT AACACTAGAG GAAATGCCAA TAAAACACAG 
AAAGATGCTG GAGTAAATGA AAAGGCAAGG GAGAAATTTT ATGAGGCTAA ACTACAGCAG 
CAACAGAGAG AACTCAAACA ATTGCAGGAA GAAAGAAAGA AACTGATTGA GATTCAAGAG 
AAAATTCAAG AATTGCAAAA GGCATGTCCT AACCTACAGC TGTCAGCTGC TAGTGTGGGT 
AATTGTCCCA CAAAAACACA TATGCCAGCT GTTACTTCAT CCCCAACCGT TAATGAAAAT 
GAAGCCAGTA CAAACAAATC TGGTTTTGAG CCTCATGATT CTTCAGTGGT AGATAATGAG 
GTATGGTCAG ATATGCGAAG ACATGAAATG TTAAGGGAGG AGCTACGACA GAGAAGAAAG 
CAGCTGGAAG CTCTGATGGC TGAACATCAG AGGAGGCAAG GTCTGGCTGA AACTGCATCT 
CCAGTGGCTG TTTCACTGAG AAGTGATGGA TCTGAGAACC TGTGTACACC TCAGCAAAGC 
AGAACAGAAA AAACAATGGC AACTTGGGGA GGTTCTACCC AGTGTGCCCT AGATGAAGAA 
GGAGATGAAG ATGGTTACCT TTCTGAAGGA ATTATTCGGA CAGACGAAGA GGAGGAAGAG 
GAGGAGCAAG ATGCCAGTTC CAATGATAAC TTTCATATGT ATCCTCCAAA CAGTGCAAAT 
CATAATTCTT ACAATGTAAA GGAAACTAAA AATAGGTGGA AGAACAATCG CCCTTTTTCA 
GCAGATGGAA ATTATCGTCC TTTAGCCAAG ACAAGACAGC AGAATATCAG CATGCAGCGT 
CAGGAAAACC TTCGCTGGGT GTCCGAACTC TCTTATGTAG AAGAGAAAGA ACAATGGCAA 
GAACAAATTA ATCAGCTAAA GAAACAACTT GATTTCAGTG TCAATATTTG TCAGACTTTA 
ATGCAAGACC AGCAGACTCT GTCTTGTCTG CTACAAACTC TTCTCACGAG TCCTTATAGT 
GTTATGCCCA GCAGTGTTGC ATCCCCGCAA GTGCACCTTA TAATGCACCA GTTGAATCAG 
TGCTATACCC AGCTAACGTG GCAGCAGAAC AATGTCCAGA GGTTGAAACA AATGCTCAGT 
GAACTCATGC GCCAACAAAA TCAGCATCCA GAAAAACCTG CAAGCAAGGA AGGAGGCAGT 
GGTACATCAC ACCCTCCTTC TCCCAGTTTA TTTTGTCCTT TCAACTTTCC AACTCAGCCT 
GTAAATCTGT TTAACCTACC GGGGTTTACT AATTTTTCGT CATTTGCACC AAGTATGAAT 
TTCAGCCCTT TATTTCCTTC TAATTTTGGA GATTTTTCTC AAAATATTTC TACACCCACT 
GAACAGCAGC AACCGTTAGC CCAGAATCCT TCAGGGAAAA CGGAATACAT GGCTTTTCCA 
AAACCTTTTG AAAGTAGTTC TTCTATTGGA GCGGAAAAAC AAAGGAATCA AAAACAGCCT 
GAAGAGGAGG TGGAGAACAG TAGGACACCA TGGTTTTATG ACCAAGAAGG TGAAGTAGAA 
AAGCCATTTC TCAAGACTGG ATTTGCAGTG TCTGTAGAGA AAACAACAAA TAGTAATCGC 
AAAAATCAAC TAGATACCAG TAGGAGAAGA CGCCAGTTTG ATGAAGTGTC CTTAGAAAGC 
TTTAACAGTA TGCTTGACCC AGTAGATCCA ACAACAGTAA CTAAAACATT CAAGTCAAGA 
AAAGCATCTG CACAGGCCAG CCTGGCATCT AAAGATAAAA CTCCCAAATC AAAGAGTAAG 
AAGAGGCATT CTACTCAGCT GAAAAGCAGA GTGAAAAATA TCGGGTATGA AAGTGCCAGT 
GTGTCTAGCA CATGTGAACC TTGCAAAAGT AGAAACAGAC ATTCAGCCCA AACTGAAGAG 
CCTGTTCAAG CAAAAGTACT CAGCAGAAAG AATCATGAGC AGCTGGAAAA AGTCATAAGA 
TGTAGTAGGT CTACAGAAAT ATCTTCAGCA CATGCTAGGA GAATACTACA GCAGTCTAAC 
AGAAATGCAT GCAATGAAGC GCCAGAAACC GGGAGTGATT TTTCCATGTT CGAAGCTTTG 
CGGGATACCA TTTATTCTGA AGTAGCTACG TTGATTTCTC AAAATGAATC TCGTCCACAT 
TTTCTTATTG AACTCTTCCA TGAGCTTCAG CTACTTAATA CAGACTATTT GAGACAGAGG 
GCCTTATATG CATTGCAGGA CATAGTATCC AGGCATATTT CTGAGAACCA TGAAAAAGAA 
GGAGAAAATA TAAAGTCCGT GAATTCTGGT ACTTGGATAG CATCCAACTC AGAACTTACT 
CCCAGTGAAA GCCTTGCTAC TACTGATGAT GAAACTTTTG AGAAGAACTT TGAGAGAGAA 
ACACATAAAA TAAGTGAGCA AAATGATGCT GATAATGCTA GTGTATCTTC AACTTGTGAG 
CCTTTTGCAA CAGATGTTCT GGGCGACACT GTGATTCACT TAGATCAGGC ATTAGCCAGA 
ATGAGAGACT ATACGCATAT GAAGATGGAG GCTGAAAGTA GTCCAGACGT CAGGTGCACC 
TGCAGGATCA TTGAGGATGA CGATGGTGCT GCTGCCACAA CCACGATTAG TAATAATTTA 
GAAGAAACTC CTGTTATTGA AAATCATAGT TCACAGCAAC CCGTGAGTGA AGTTGCTACC 
GTCCCGTGTC CTAGAATTGA TACTCAGCAA CTGGACCGGC AAATTAAAGC AATTATGAAA 
GAAGTCATTC CTTTTTTGAA GGAGCACATG GATGAAGTAT GTTCTTCTCA ACTTCTAACC 
TCAGTAAGAC GCATGGTTTT GGTCCTTACT CAGCAAAACA ATGAGAGCAA AGAGTTTGTA 
AAGTTCTTTC ATAAGCAGCT TGGAAGTATA TTACAGGATT CACTCGCAAA ATTTGCTGGC 
AGAAAACTGA AGGATTGTGG AGAAGATCTT CTTGTAGAGA TATCTGAAGT GTTATTTAAT 
GAATTGGCTT TCTTTAAGCT CATGCAAGAT TTGGATAATA ATAGTGTAAC TGTTAAACAG 
AGATGCAAAA GGAAAATAGA AGCTGCTGGA GTGATACAGT CTTATGCAAA AGAGGACAAG 
GATGAGACGG AAACAGTTAA GCAGACTCAA ACGTCTGAGG TTTATGATGG TGACGGCCCC 
AAAAATGTGA GATCTGATGT TTCTGATCAA GAGGATGATG AAGAAAGTGA AGAATGTCCA 
GTGTCTATTA ATTTGTCCAA AGCTGAAACT CAGGCTTTGA CTAATTACGG AAGTGGAGAA 
GATGAGAATG AAGATGAAGA AATAGAAGAA TTTGAAGAGG GACCTGTAGA TGTCCAGACG 
TCACTTCAGG CTAACACTGA AACTACAGAA GAAAATGAAC ACGATGATCA GGTTCCACAG 
CATGATTTTG AAAAATCATC AGAAAGCAAA AATGTCCCAT CAGAACAAGA ACCCACTACT 
AGTAAAGGTG ACCAGAATAG CACTCCTGTG AAGCCCTGTT ACCTCAGTAT CGTGGAAAAT 
GAGCAACCTT TAAATAGCCC TGTCCAGAAG GACACTCTCA CTACCATCGA CTCATCCAGT 
CAGCCTAACC CTTTGCCTTT ACCTTTAACT GAAATTGAAA CCTTGGTGCC TAGAGTCAAA 
GAAGTGAAAT CTGCTCAGGA GACTCCTGAA AGTTCTTTGG CTGGAAGTCC TGATACTGAA 
TCTCCAGTGT TAGTGAATGA CTATGAAGCA GAATCTGGTA ACATAAGTCA AAAATCTGAT 
GAAGAAGACT TTGTGAAAGT TGAAGATTTA CCACTTAAAC TGACAATATA TTCAGAGGCA 
GATCTAAGGA AGAAAATGGC CGAAGAAGAA CAGAAAAACC ACTTATCTGG TGAAATATGT 
GCAATGCAGA CTGAAGAATT AGCTGGGAAT TCTCAGACAC TAAAAGAACC TGAGCAAGTA 
TGTGCTCAGT CTGCCATCTT AAGATGGAAA CCCTTACCTG TGCCTTTTAA ACTGTTGGTG 
GTGTCATCAG GGAATTATTG TAGCAAAGTA AGTTTCTTTT CTAATAACTT TATATTAAAA 
TTGAAAGTTT TAAATGGAAG AGAACAATAT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006080200.1
CDS	113..6625
Translation	MATGGGPFEEGVNDQDLPNWSNESVDDRLNNMDWGSQQKKANRSSEKNKRKFGVESDKRVTNDISPESSPGVGRRRTKTSLTFPHSRYMTQMSVPEQAELEKLKQRINFSDLDQINTNKSKDATVSPQKREMIGSAQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIREYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLQKILARENEEEDVRTIDSAVGSGSVAESTSLNIDVRSEASDATEASFSLRIRPCVEDKLGNSASQAQVSDTDVTTSPKGKGDRPPQHDRELRPNRKCSRKRGFPSKARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSEKVAETTPGHHTVPGRQPAHSPLHQRVPIRETTGSLSGVSITSELNEELNDLIQHFHNQLRDSQPPAVPDNRRQAESLSLTREISQSRNPSVSEHLPDEKVQLFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSFVPSSASPQRSVDQRSTTTAPSAPVVLTPVVNGESNNLTPSVPYPAASLVSQNQNENEGHLNPTEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENTKEEETEESEYDSEHENSEPVTNIRNPQVAATWNEVNSNSNAQCVSNNRDGRAVNSNCEINNRSAANIRALNMPPLSADCRYNREGEQEMPVAQGHEEEEEEEEEAEDEALSGASLSSHSSSLIIETPEDAEFEHKIQKLMAAKLKLRHLQNLVAMVQDDDAANQGVTSVEDFFPAEDTKQNANNTRGNANKTQKDAGVNEKAREKFYEAKLQQQQRELKQLQEERKKLIEIQEKIQELQKACPNLQLSAASVGNCPTKTHMPAVTSSPTVNENEASTNKSGFEPHDSSVVDNEVWSDMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETASPVAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGIIRTDEEEEEEEQDASSNDNFHMYPPNSANHNSYNVKETKNRWKNNRPFSADGNYRPLAKTRQQNISMQRQENLRWVSELSYVEEKEQWQEQINQLKKQLDFSVNICQTLMQDQQTLSCLLQTLLTSPYSVMPSSVASPQVHLIMHQLNQCYTQLTWQQNNVQRLKQMLSELMRQQNQHPEKPASKEGGSGTSHPPSPSLFCPFNFPTQPVNLFNLPGFTNFSSFAPSMNFSPLFPSNFGDFSQNISTPTEQQQPLAQNPSGKTEYMAFPKPFESSSSIGAEKQRNQKQPEEEVENSRTPWFYDQEGEVEKPFLKTGFAVSVEKTTNSNRKNQLDTSRRRRQFDEVSLESFNSMLDPVDPTTVTKTFKSRKASAQASLASKDKTPKSKSKKRHSTQLKSRVKNIGYESASVSSTCEPCKSRNRHSAQTEEPVQAKVLSRKNHEQLEKVIRCSRSTEISSAHARRILQQSNRNACNEAPETGSDFSMFEALRDTIYSEVATLISQNESRPHFLIELFHELQLLNTDYLRQRALYALQDIVSRHISENHEKEGENIKSVNSGTWIASNSELTPSESLATTDDETFEKNFERETHKISEQNDADNASVSSTCEPFATDVLGDTVIHLDQALARMRDYTHMKMEAESSPDVRCTCRIIEDDDGAAATTTISNNLEETPVIENHSSQQPVSEVATVPCPRIDTQQLDRQIKAIMKEVIPFLKEHMDEVCSSQLLTSVRRMVLVLTQQNNESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSVTVKQRCKRKIEAAGVIQSYAKEDKDETETVKQTQTSEVYDGDGPKNVRSDVSDQEDDEESEECPVSINLSKAETQALTNYGSGEDENEDEEIEEFEEGPVDVQTSLQANTETTEENEHDDQVPQHDFEKSSESKNVPSEQEPTTSKGDQNSTPVKPCYLSIVENEQPLNSPVQKDTLTTIDSSSQPNPLPLPLTEIETLVPRVKEVKSAQETPESSLAGSPDTESPVLVNDYEAESGNISQKSDEEDFVKVEDLPLKLTIYSEADLRKKMAEEEQKNHLSGEICAMQTEELAGNSQTLKEPEQVCAQSAILRWKPLPVPFKLLVVSSGNYCSKVSFFSNNFILKLKVLNGREQY

Target ORF information:

RefSeq Version	XM_006080138.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis pericentriolar material 1 (PCM1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006080138.1

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
AGCAATGAGA GTGTTGATGA CCGACTCAAC AATATGGATT GGGGCAGCCA GCAGAAGAAG 
GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA 
ACTAACGATA TTTCTCCGGA GTCATCACCG GGAGTTGGAA GACGAAGAAC AAAGACTTCA 
CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TCCCCGAGCA GGCAGAACTA 
GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGATTAATAC TAACAAGAGC 
AAAGATGCCA CTGTAAGTCC TCAAAAGAGA GAAATGATTG GATCAGCACA GTGTAAAGAG 
TTGTTTGCTT CTGCTTTAAG TAATGACCTT TTGCAAAACT GTCAAGTCTC TGAAGAAGAT 
GGAAGAGGAG AACCTGCAAT GGAGAGCAGC CAGATTGTAA GCAGGCTTGT TCAGATTCGT 
GAGTATATTA CTAAAGCTAG TTCCATGCGG GAAGACCTTG TAGAGAAAAA TGAAAGATCT 
GCTAATGTTG AGCGCCTCAC TCATCTAATA GATCACCTTA AAGAACAAGA AAAGTCATAT 
ATGAAATTTC TCCAGAAGAT TCTTGCTAGA GAAAATGAGG AGGAGGATGT TCGGACTATA 
GATTCAGCTG TGGGATCTGG TTCTGTAGCT GAGAGCACAT CGCTAAACAT AGATGTGCGG 
TCTGAGGCTT CAGATGCCAC GGAGGCATCT TTTAGTCTGA GAATTCGGCC GTGCGTTGAG 
GACAAACTAG GGAATTCAGC TTCACAAGCA CAGGTTTCAG ACACTGACGT TACTACAAGT 
CCAAAAGGGA AAGGTGACAG ACCTCCTCAG CATGACAGGG AGCTGAGGCC TAATAGGAAA 
TGTAGCCGAA AACGTGGATT TCCCTCAAAG GCCAGAGATC CTCAGCAGGA GCCTATGGAA 
GAGATAGAAA ATTTGAAGAA ACAACATGAT TTATTAAAAA GGATGTTACA GCAGCAGGAG 
CAGCTGAGAG CTCTACAAGG ACGACAAGCT GCTCTGCTTG CCCTGCAGCA TAAAGCTGAG 
CAGGCTATAG CTGTGATGGA TGATTCTGAA AAGGTTGCGG AGACAACTCC TGGCCATCAT 
ACTGTACCAG GCAGACAGCC GGCTCATTCA CCTTTGCATC AGAGAGTACC CATAAGAGAA 
ACTACAGGTA GCCTATCTGG AGTCAGTATC ACCTCTGAAC TAAACGAAGA ATTGAATGAT 
TTAATTCAGC ATTTTCATAA TCAGCTTCGT GATTCTCAGC CTCCAGCTGT TCCAGACAAT 
AGAAGACAAG CAGAAAGTCT TTCATTAACT AGGGAGATTT CTCAGAGCAG AAATCCTTCA 
GTTTCAGAGC ATTTACCTGA TGAGAAAGTC CAACTTTTTA GCAAGATGAG AGTGTTACAA 
GAAAAGAAAC AAAAAATGGA CAAACTTCTT GGAGAACTTC ATACACTTCG AGATCAGCAT 
TTGAACAATT CATCATTTGT GCCTTCTTCA GCCTCTCCAC AAAGGAGTGT GGATCAGAGA 
AGTACAACCA CAGCTCCTTC TGCTCCTGTA GTCTTAACAC CAGTGGTCAA TGGAGAATCC 
AATAACCTCA CGCCATCTGT TCCTTATCCT GCTGCTTCTC TAGTTTCTCA GAATCAGAAT 
GAAAATGAAG GCCACCTAAA TCCAACTGAA AAACTCCAGA AGTTAAATGA AGTTCGAAAG 
AGGTTGAATG AACTAAGAGA ATTAGTTCAT TATTATGAGC AAACATCAGA TATGATGACA 
GATGCTGTGA ATGAAAACAC AAAAGAAGAA GAAACTGAAG AATCAGAATA TGATTCGGAG 
CATGAAAACT CGGAACCTGT TACTAACATT CGAAATCCAC AAGTGGCTGC CACCTGGAAC 
GAAGTAAATA GTAATTCTAA TGCACAGTGT GTTTCCAACA ATAGAGATGG GCGAGCAGTT 
AATTCTAACT GTGAAATTAA CAACAGGTCT GCTGCCAACA TAAGGGCTCT AAACATGCCT 
CCTCTTTCAG CAGATTGTCG ATACAATAGA GAAGGAGAAC AGGAGATGCC TGTTGCACAA 
GGTCATGAGG AGGAGGAGGA GGAGGAGGAG GAAGCAGAAG ATGAGGCCCT CAGTGGAGCT 
TCACTGTCTA GTCACAGCAG CAGCCTGATC ATTGAGACTC CTGAAGATGC TGAATTTGAA 
CATAAGATCC AGAAGCTTAT GGCTGCAAAA CTGAAACTTA GACACTTACA GAACCTTGTA 
GCCATGGTGC AGGACGATGA TGCAGCCAAT CAAGGAGTTA CTTCTGTGGA GGATTTCTTC 
CCAGCAGAAG ACACCAAACA AAATGCAAAT AACACTAGAG GAAATGCCAA TAAAACACAG 
AAAGATGCTG GAGTAAATGA AAAGGCAAGG GAGAAATTTT ATGAGGCTAA ACTACAGCAG 
CAACAGAGAG AACTCAAACA ATTGCAGGAA GAAAGAAAGA AACTGATTGA GATTCAAGAG 
AAAATTCAAG AATTGCAAAA GGCATGTCCT AACCTACAGC TGTCAGCTGC TAGTGTGGGT 
AATTGTCCCA CAAAAACACA TATGCCAGCT GTTACTTCAT CCCCAACCGT TAATGAAAAT 
GAAGCCAGTA CAAACAAATC TGGTTTTGAG CCTCATGATT CTTCAGTGGT AGATAATGAG 
GTATGGTCAG ATATGCGAAG ACATGAAATG TTAAGGGAGG AGCTACGACA GAGAAGAAAG 
CAGCTGGAAG CTCTGATGGC TGAACATCAG AGGAGGCAAG GTCTGGCTGA AACTGCATCT 
CCAGTGGCTG TTTCACTGAG AAGTGATGGA TCTGAGAACC TGTGTACACC TCAGCAAAGC 
AGAACAGAAA AAACAATGGC AACTTGGGGA GGTTCTACCC AGTGTGCCCT AGATGAAGAA 
GGAGATGAAG ATGGTTACCT TTCTGAAGGA ATTATTCGGA CAGACGAAGA GGAGGAAGAG 
GAGGAGCAAG ATGCCAGTTC CAATGATAAC TTTCATATGT ATCCTCCAAA CAGTGCAAAT 
CATAATTCTT ACAATGTAAA GGAAACTAAA AATAGGTGGA AGAACAATCG CCCTTTTTCA 
GCAGATGGAA ATTATCGTCC TTTAGCCAAG ACAAGACAGC AGAATATCAG CATGCAGCGT 
CAGGAAAACC TTCGCTGGGT GTCCGAACTC TCTTATGTAG AAGAGAAAGA ACAATGGCAA 
GAACAAATTA ATCAGCTAAA GAAACAACTT GATTTCAGTG TCAATATTTG TCAGACTTTA 
ATGCAAGACC AGCAGACTCT GTCTTGTCTG CTACAAACTC TTCTCACGAG TCCTTATAGT 
GTTATGCCCA GCAGTGTTGC ATCCCCGCAA GTGCACCTTA TAATGCACCA GTTGAATCAG 
TGCTATACCC AGCTAACGTG GCAGCAGAAC AATGTCCAGA GGTTGAAACA AATGCTCAGT 
GAACTCATGC GCCAACAAAA TCAGCATCCA GAAAAACCTG CAAGCAAGGA AGGAGGCAGT 
GGTACATCAC ACCCTCCTTC TCCCAGTTTA TTTTGTCCTT TCAACTTTCC AACTCAGCCT 
GTAAATCTGT TTAACCTACC GGGGTTTACT AATTTTTCGT CATTTGCACC AAGTATGAAT 
TTCAGCCCTT TATTTCCTTC TAATTTTGGA GATTTTTCTC AAAATATTTC TACACCCACT 
GAACAGCAGC AACCGTTAGC CCAGAATCCT TCAGGGAAAA CGGAATACAT GGCTTTTCCA 
AAACCTTTTG AAAGTAGTTC TTCTATTGGA GCGGAAAAAC AAAGGAATCA AAAACAGCCT 
GAAGAGGAGG TGGAGAACAG TAGGACACCA TGGTTTTATG ACCAAGAAGG TGAAGTAGAA 
AAGCCATTTC TCAAGACTGG ATTTGCAGTG TCTGTAGAGA AAACAACAAA TAGTAATCGC 
AAAAATCAAC TAGATACCAG TAGGAGAAGA CGCCAGTTTG ATGAAGTGTC CTTAGAAAGC 
TTTAACAGTA TGCTTGACCC AGTAGATCCA ACAACAGTAA CTAAAACATT CAAGTCAAGA 
AAAGCATCTG CACAGGCCAG CCTGGCATCT AAAGATAAAA CTCCCAAATC AAAGAGTAAG 
AAGAGGCATT CTACTCAGCT GAAAAGCAGA GTGAAAAATA TCGGGTATGA AAGTGCCAGT 
GTGTCTAGCA CATGTGAACC TTGCAAAAGT AGAAACAGAC ATTCAGCCCA AACTGAAGAG 
CCTGTTCAAG CAAAAGTACT CAGCAGAAAG AATCATGAGC AGCTGGAAAA AGTCATAAGA 
TGTAGTAGGT CTACAGAAAT ATCTTCAGCA CATGCTAGGA GAATACTACA GCAGTCTAAC 
AGAAATGCAT GCAATGAAGC GCCAGAAACC GGGAGTGATT TTTCCATGTT CGAAGCTTTG 
CGGGATACCA TTTATTCTGA AGTAGCTACG TTGATTTCTC AAAATGAATC TCGTCCACAT 
TTTCTTATTG AACTCTTCCA TGAGCTTCAG CTACTTAATA CAGACTATTT GAGACAGAGG 
GCCTTATATG CATTGCAGGA CATAGTATCC AGGCATATTT CTGAGAACCA TGAAAAAGAA 
GGAGAAAATA TAAAGTCCGT GAATTCTGGT ACTTGGATAG CATCCAACTC AGAACTTACT 
CCCAGTGAAA GCCTTGCTAC TACTGATGAT GAAACTTTTG AGAAGAACTT TGAGAGAGAA 
ACACATAAAA TAAGTGAGCA AAATGATGCT GATAATGCTA GTGTATCTTC AACTTGTGAG 
CCTTTTGCAA CAGATGTTCT GGGCGACACT GTGATTCACT TAGATCAGGC ATTAGCCAGA 
ATGAGAGACT ATACGCATAT GAAGATGGAG GCTGAAAGTA GTCCAGACGT CAGGTGCACC 
TGCAGGATCA TTGAGGATGA CGATGGTGCT GCTGCCACAA CCACGATTAG TAATAATTTA 
GAAGAAACTC CTGTTATTGA AAATCATAGT TCACAGCAAC CCGTGAGTGA AGTTGCTACC 
GTCCCGTGTC CTAGAATTGA TACTCAGCAA CTGGACCGGC AAATTAAAGC AATTATGAAA 
GAAGTCATTC CTTTTTTGAA GGAGCACATG GATGAAGTAT GTTCTTCTCA ACTTCTAACC 
TCAGTAAGAC GCATGGTTTT GGTCCTTACT CAGCAAAACA ATGAGAGCAA AGAGTTTGTA 
AAGTTCTTTC ATAAGCAGCT TGGAAGTATA TTACAGGATT CACTCGCAAA ATTTGCTGGC 
AGAAAACTGA AGGATTGTGG AGAAGATCTT CTTGTAGAGA TATCTGAAGT GTTATTTAAT 
GAATTGGCTT TCTTTAAGCT CATGCAAGAT TTGGATAATA ATAGTGTAAC TGTTAAACAG 
AGATGCAAAA GGAAAATAGA AGCTGCTGGA GTGATACAGT CTTATGCAAA AGAGGACAAG 
GATGAGACGG AAACAGTTAA GCAGACTCAA ACGTCTGAGG TTTATGATGG TGACGGCCCC 
AAAAATGTGA GATCTGATGT TTCTGATCAA GAGGATGATG AAGAAAGTGA AGAATGTCCA 
GTGTCTATTA ATTTGTCCAA AGCTGAAACT CAGGCTTTGA CTAATTACGG AAGTGGAGAA 
GATGAGAATG AAGATGAAGA AATAGAAGAA TTTGAAGAGG GACCTGTAGA TGTCCAGACG 
TCACTTCAGG CTAACACTGA AACTACAGAA GAAAATGAAC ACGATGATCA GGTTCCACAG 
CATGATTTTG AAAAATCATC AGAAAGCAAA AATGTCCCAT CAGAACAAGA ACCCACTACT 
AGTAAAGGTG ACCAGAATAG CACTCCTGTG AAGCCCTGTT ACCTCAGTAT CGTGGAAAAT 
GAGCAACCTT TAAATAGCCC TGTCCAGAAG GACACTCTCA CTACCATCGA CTCATCCAGT 
CAGCCTAACC CTTTGCCTTT ACCTTTAACT GAAATTGAAA CCTTGGTGCC TAGAGTCAAA 
GAAGTGAAAT CTGCTCAGGA GACTCCTGAA AGTTCTTTGG CTGGAAGTCC TGATACTGAA 
TCTCCAGTGT TAGTGAATGA CTATGAAGCA GAATCTGGTA ACATAAGTCA AAAATCTGAT 
GAAGAAGACT TTGTGAAAGT TGAAGATTTA CCACTTAAAC TGACAATATA TTCAGAGGCA 
GATCTAAGGA AGAAAATGGC CGAAGAAGAA CAGAAAAACC ACTTATCTGG TGAAATATGT 
GCAATGCAGA CTGAAGAATT AGCTGGGAAT TCTCAGACAC TAAAAGAACC TGAGCAAGTA 
TGTGCTCAGT CTGCCATCTT AAGATGGAAA CCCTTACCTG TGCCTTTTAA ACTGTTGGTG 
GTGTCATCAG GGAATTATTG TAGCAAAGTA AGTTTCTTTT CTAATAACTT TATATTAAAA 
TTGAAAGTTT TAAATGGAAG AGAACAATAT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( XM_006080139.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006080201.1 Bubalus bubalis pericentriolar material 1 (PCM1), transcript variant X6, mRNA.

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CloneID	OBu33066
Clone ID Related Accession (Same CDS sequence)	XM_006080139.1
Accession Version	XM_006080139.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6471bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	pericentriolar material 1 protein isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785878.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG GAGGAGGTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
AGCAATGAGA GTGTTGATGA CCGACTCAAC AATATGGATT GGGGCAGCCA GCAGAAGAAG 
GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA 
ACTAACGATA TTTCTCCGGA GTCATCACCG GGAGTTGGAA GACGAAGAAC AAAGACTTCA 
CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TCCCCGAGCA GGCAGAACTA 
GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGATTAATAC TAACAAGAGC 
AAAGATGCCA CTGTAAGTCC TCAAAAGAGA GAAATGATTG GATCAGCACA GTGTAAAGAG 
TTGTTTGCTT CTGCTTTAAG TAATGACCTT TTGCAAAACT GTCAAGTCTC TGAAGAAGAT 
GGAAGAGGAG AACCTGCAAT GGAGAGCAGC CAGATTGTAA GCAGGCTTGT TCAGATTCGT 
GAGTATATTA CTAAAGCTAG TTCCATGCGG GAAGACCTTG TAGAGAAAAA TGAAAGATCT 
GCTAATGTTG AGCGCCTCAC TCATCTAATA GATCACCTTA AAGAACAAGA AAAGTCATAT 
ATGAAATTTC TCCAGAAGAT TCTTGCTAGA GAAAATGAGG AGGAGGATGT TCGGACTATA 
GATTCAGCTG TGGGATCTGG TTCTGTAGCT GAGAGCACAT CGCTAAACAT AGATGTGCGG 
TCTGAGGCTT CAGATGCCAC GGAGGCATCT TTTAGTCTGA GAATTCGGCC GTGCGTTGAG 
GACAAACTAG GGAATTCAGC TTCACAAGCA CAGGTTTCAG ACACTGACGT TACTACAAGT 
CCAAAAGGGA AAGGTGACAG ACCTCCTCAG CATGACAGGG AGCTGAGGCC TAATAGGAAA 
TGTAGCCGAA AACGTGGATT TCCCTCAAAG GCCAGAGATC CTCAGCAGGA GCCTATGGAA 
GAGATAGAAA ATTTGAAGAA ACAACATGAT TTATTAAAAA GGATGTTACA GCAGCAGGAG 
CAGCTGAGAG CTCTACAAGG ACGACAAGCT GCTCTGCTTG CCCTGCAGCA TAAAGCTGAG 
CAGGCTATAG CTGTGATGGA TGATTCTGAA AAGGTTGCGG AGACAACTCC TGGCCATCAT 
ACTGTACCAG GCAGACAGCC GGCTCATTCA CCTTTGCATC AGAGAGTACC CATAAGAGAA 
ACTACAGGTA GCCTATCTGG AGTCAGTATC ACCTCTGAAC TAAACGAAGA ATTGAATGAT 
TTAATTCAGC ATTTTCATAA TCAGCTTCGT GATTCTCAGC CTCCAGCTGT TCCAGACAAT 
AGAAGACAAG CAGAAAGTCT TTCATTAACT AGGGAGATTT CTCAGAGCAG AAATCCTTCA 
GTTTCAGAGC ATTTACCTGA TGAGAAAGTC CAACTTTTTA GCAAGATGAG AGTGTTACAA 
GAAAAGAAAC AAAAAATGGA CAAACTTCTT GGAGAACTTC ATACACTTCG AGATCAGCAT 
TTGAACAATT CATCATTTGT GCCTTCTTCA GCCTCTCCAC AAAGGAGTGT GGATCAGAGA 
AGTACAACCA CAGCTCCTTC TGCTCCTGTA GTCTTAACAC CAGTGGTCAA TGGAGAATCC 
AATAACCTCA CGCCATCTGT TCCTTATCCT GCTGCTTCTC TAGTTTCTCA GAATCAGAAT 
GAAAATGAAG GCCACCTAAA TCCAACTGAA AAACTCCAGA AGTTAAATGA AGTTCGAAAG 
AGGTTGAATG AACTAAGAGA ATTAGTTCAT TATTATGAGC AAACATCAGA TATGATGACA 
GATGCTGTGA ATGAAAACAC AAAAGAAGAA GAAACTGAAG AATCAGAATA TGATTCGGAG 
CATGAAAACT CGGAACCTGT TACTAACATT CGAAATCCAC AAGTGGCTGC CACCTGGAAC 
GAAGTAAATA GTAATTCTAA TGCACAGTGT GTTTCCAACA ATAGAGATGG GCGAGCAGTT 
AATTCTAACT GTGAAATTAA CAACAGGTCT GCTGCCAACA TAAGGGCTCT AAACATGCCT 
CCTCTTTCAG CAGATTGTCG ATACAATAGA GAAGGAGAAC AGGAGATGCC TGTTGCACAA 
GGTCATGAGG AGGAGGAGGA GGAGGAGGAG GAAGCAGAAG ATGAGGCCCT CAGTGGAGCT 
TCACTGTCTA GTCACAGCAG CAGCCTGATC ATTGAGACTC CTGAAGATGC TGAATTTGAA 
CATAAGATCC AGAAGCTTAT GGCTGCAAAA CTGAAACTTA GACACTTACA GAACCTTGTA 
GCCATGGTGC AGGACGATGA TGCAGCCAAT CAAGGAGTTA CTTCTGTGGA GGATTTCTTC 
CCAGCAGAAG ACACCAAACA AAATGCAAAT AACACTAGAG GAAATGCCAA TAAAACACAG 
AAAGATGCTG GAGTAAATGA AAAGGCAAGG GAGAAATTTT ATGAGGCTAA ACTACAGCAG 
CAACAGAGAG AACTCAAACA ATTGCAGGAA GAAAGAAAGA AACTGATTGA GATTCAAGAG 
AAAATTCAAG AATTGCAAAA GGCATGTCCT AACCTACAGC TGTCAGCTGC TAGTGTGGGT 
AATTGTCCCA CAAAAACACA TATGCCAGCT GTTACTTCAT CCCCAACCGT TAATGAAAAT 
GAAGCCAGTA CAAACAAATC TGGTTTTGAG CCTCATGATT CTTCAGTGGT AGATAATGAG 
GTATGGTCAG ATATGCGAAG ACATGAAATG TTAAGGGAGG AGCTACGACA GAGAAGAAAG 
CAGCTGGAAG CTCTGATGGC TGAACATCAG AGGAGGCAAG GTCTGGCTGA AACTGCATCT 
CCAGTGGCTG TTTCACTGAG AAGTGATGGA TCTGAGAACC TGTGTACACC TCAGCAAAGC 
AGAACAGAAA AAACAATGGC AACTTGGGGA GGTTCTACCC AGTGTGCCCT AGATGAAGAA 
GGAGATGAAG ATGGTTACCT TTCTGAAGGA ATTATTCGGA CAGACGAAGA GGAGGAAGAG 
GAGGAGCAAG ATGCCAGTTC CAATGATAAC TTTCATATGT ATCCTCCAAA CAGTGCAAAT 
CATAATTCTT ACAATGTAAA GGAAACTAAA AATAGGTGGA AGAACAATCG CCCTTTTTCA 
GCAGATGGAA ATTATCGTCC TTTAGCCAAG ACAAGACAGC AGAATATCAG CATGCAGCGT 
CAGGAAAACC TTCGCTGGGT GTCCGAACTC TCTTATGTAG AAGAGAAAGA ACAATGGCAA 
GAACAAATTA ATCAGCTAAA GAAACAACTT GATTTCAGTG TCAATATTTG TCAGACTTTA 
ATGCAAGACC AGCAGACTCT GTCTTGTCTG CTACAAACTC TTCTCACGAG TCCTTATAGT 
GTTATGCCCA GCAGTGTTGC ATCCCCGCAA GTGCACCTTA TAATGCACCA GTTGAATCAG 
TGCTATACCC AGCTAACGTG GCAGCAGAAC AATGTCCAGA GGTTGAAACA AATGCTCAGT 
GAACTCATGC GCCAACAAAA TCAGCATCCA GAAAAACCTG CAAGCAAGGA AGGAGGCAGT 
GGTACATCAC ACCCTCCTTC TCCCAGTTTA TTTTGTCCTT TCAACTTTCC AACTCAGCCT 
GTAAATCTGT TTAACCTACC GGGGTTTACT AATTTTTCGT CATTTGCACC AAGTATGAAT 
TTCAGCCCTT TATTTCCTTC TAATTTTGGA GATTTTTCTC AAAATATTTC TACACCCACT 
GAACAGCAGC AACCGTTAGC CCAGAATCCT TCAGGGAAAA CGGAATACAT GGCTTTTCCA 
AAACCTTTTG AAAGTAGTTC TTCTATTGGA GCGGAAAAAC AAAGGAATCA AAAACAGCCT 
GAAGAGGAGG TGGAGAACAG TAGGACACCA TGGTTTTATG ACCAAGAAGG TGAAGTAGAA 
AAGCCATTTC TCAAGACTGG ATTTGCAGTG TCTGTAGAGA AAACAACAAA TAGTAATCGC 
AAAAATCAAC TAGATACCAG TAGGAGAAGA CGCCAGTTTG ATGAAGTGTC CTTAGAAAGC 
TTTAACAGTA TGCTTGACCC AGTAGATCCA ACAACAGTAA CTAAAACATT CAAGTCAAGA 
AAAGCATCTG CACAGGCCAG CCTGGCATCT AAAGATAAAA CTCCCAAATC AAAGAGTAAG 
AAGAGGCATT CTACTCAGCT GAAAAGCAGA GTGAAAAATA TCGGGTATGA AAGTGCCAGT 
GTGTCTAGCA CATGTGAACC TTGCAAAAGT AGAAACAGAC ATTCAGCCCA AACTGAAGAG 
CCTGTTCAAG CAAAAGTACT CAGCAGAAAG AATCATGAGC AGCTGGAAAA AGTCATAAGA 
TGTAGTAGGT CTACAGAAAT ATCTTCAGCA CATGCTAGGA GAATACTACA GCAGTCTAAC 
AGAAATGCAT GCAATGAAGC GCCAGAAACC GGGAGTGATT TTTCCATGTT CGAAGCTTTG 
CGGGATACCA TTTATTCTGA AGTAGCTACG TTGATTTCTC AAAATGAATC TCGTCCACAT 
TTTCTTATTG AACTCTTCCA TGAGCTTCAG CTACTTAATA CAGACTATTT GAGACAGAGG 
GCCTTATATG CATTGCAGGA CATAGTATCC AGGCATATTT CTGAGAACCA TGAAAAAGAA 
GGAGAAAATA TAAAGTCCGT GAATTCTGGT ACTTGGATAG CATCCAACTC AGAACTTACT 
CCCAGTGAAA GCCTTGCTAC TACTGATGAT GAAACTTTTG AGAAGAACTT TGAGAGAGAA 
ACACATAAAA TAAGTGAGCA AAATGATGCT GATAATGCTA GTGTATCTTC AACTTGTGAG 
CCTTTTGCAA CAGATGTTCT GGGCGACACT GTGATTCACT TAGATCAGGC ATTAGCCAGA 
ATGAGAGACT ATACGCATAT GAAGATGGAG GCTGAAAGTA GTCCAGACGT CAGGTGCACC 
TGCAGGATCA TTGAGGATGA CGATGGTGCT GCTGCCACAA CCACGATTAG TAATAATTTA 
GAAGAAACTC CTGTTATTGA AAATCATAGT TCACAGCAAC CCGTGAGTGA AGTTGCTACC 
GTCCCGTGTC CTAGAATTGA TACTCAGCAA CTGGACCGGC AAATTAAAGC AATTATGAAA 
GAAGTCATTC CTTTTTTGAA GGAGCACATG GATGAAGTAT GTTCTTCTCA ACTTCTAACC 
TCAGTAAGAC GCATGGTTTT GGTCCTTACT CAGCAAAACA ATGAGAGCAA AGAGTTTGTA 
AAGTTCTTTC ATAAGCAGCT TGGAAGTATA TTACAGGATT CACTCGCAAA ATTTGCTGGC 
AGAAAACTGA AGGATTGTGG AGAAGATCTT CTTGTAGAGA TATCTGAAGT GTTATTTAAT 
GAATTGGCTT TCTTTAAGCT CATGCAAGAT TTGGATAATA ATAGTGTAAC TGTTAAACAG 
AGATGCAAAA GGAAAATAGA AGCTGCTGGA GTGATACAGT CTTATGCAAA AGAGGCCAAA 
AGGATTCTTG AAGGAGATCA CAGTTCACCT GCTGGAGAAA TTGATGATGA AGACAAAGAC 
AAGGATGAGA CGGAAACAGT TAAGCAGACT CAAACGTCTG AGGTTTATGA TGGTGACGGC 
CCCAAAAATG TGAGATCTGA TGTTTCTGAT CAAGAGGATG ATGAAGAAAG TGAAGAATGT 
CCAGTGTCTA TTAATTTGTC CAAAGCTGAA ACTCAGGCTT TGACTAATTA CGGAAGTGGA 
GAAGATGAGA ATGAAGATGA AGAAATAGAA GAATTTGAAG AGGGACCTGT AGATGTCCAG 
ACGTCACTTC AGGCTAACAC TGAAACTACA GAAGAAAATG AACACGATGA TCAGGTTCCA 
CAGCATGATT TTGAAAAATC ATCAGAAAGC AAAAATGTCC CATCAGAACA AGAACCCACT 
ACTAGTAAAG GTGACCAGAA TAGCACTCCT GTGAAGCCCT GTTACCTCAG TATCGTGGAA 
AATGAGCAAC CTTTAAATAG CCCTGTCCAG AAGGACACTC TCACTACCAT CGACTCATCC 
AGTCAGCCTA ACCCTTTGCC TTTACCTTTA ACTGAAATTG AAACCTTGGT GCCTAGAGTC 
AAAGAAGTGA AATCTGCTCA GGAGACTCCT GAAAGTTCTT TGGCTGGAAG TCCTGATACT 
GAATCTCCAG TGTTAGTGAA TGACTATGAA GCAGAATCTG GTAACATAAG TCAAAAATCT 
GATGAAGAAG ACTTTGTGAA AGTTGAAGAT TTACCACTTA AACTGACAAT ATATTCAGAG 
GCAGATCTAA GGAAGAAAAT GGCCGAAGAA GAACAGAAAA ACCACTTATC TGGTGAAATA 
TGTGCAATGC AGACTGAAGA ATTAGCTGGG AATTCTCAGA CACTAAAAGA ACCTGGGTCC 
CACCACATTT TTAGTTGCCT ATCCCTGCCT CCAAATGTCA GACCAAAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006080201.1
CDS	113..6583
Translation	MATGGGPFEEGVNDQDLPNWSNESVDDRLNNMDWGSQQKKANRSSEKNKRKFGVESDKRVTNDISPESSPGVGRRRTKTSLTFPHSRYMTQMSVPEQAELEKLKQRINFSDLDQINTNKSKDATVSPQKREMIGSAQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIREYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLQKILARENEEEDVRTIDSAVGSGSVAESTSLNIDVRSEASDATEASFSLRIRPCVEDKLGNSASQAQVSDTDVTTSPKGKGDRPPQHDRELRPNRKCSRKRGFPSKARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSEKVAETTPGHHTVPGRQPAHSPLHQRVPIRETTGSLSGVSITSELNEELNDLIQHFHNQLRDSQPPAVPDNRRQAESLSLTREISQSRNPSVSEHLPDEKVQLFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSFVPSSASPQRSVDQRSTTTAPSAPVVLTPVVNGESNNLTPSVPYPAASLVSQNQNENEGHLNPTEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENTKEEETEESEYDSEHENSEPVTNIRNPQVAATWNEVNSNSNAQCVSNNRDGRAVNSNCEINNRSAANIRALNMPPLSADCRYNREGEQEMPVAQGHEEEEEEEEEAEDEALSGASLSSHSSSLIIETPEDAEFEHKIQKLMAAKLKLRHLQNLVAMVQDDDAANQGVTSVEDFFPAEDTKQNANNTRGNANKTQKDAGVNEKAREKFYEAKLQQQQRELKQLQEERKKLIEIQEKIQELQKACPNLQLSAASVGNCPTKTHMPAVTSSPTVNENEASTNKSGFEPHDSSVVDNEVWSDMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETASPVAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGIIRTDEEEEEEEQDASSNDNFHMYPPNSANHNSYNVKETKNRWKNNRPFSADGNYRPLAKTRQQNISMQRQENLRWVSELSYVEEKEQWQEQINQLKKQLDFSVNICQTLMQDQQTLSCLLQTLLTSPYSVMPSSVASPQVHLIMHQLNQCYTQLTWQQNNVQRLKQMLSELMRQQNQHPEKPASKEGGSGTSHPPSPSLFCPFNFPTQPVNLFNLPGFTNFSSFAPSMNFSPLFPSNFGDFSQNISTPTEQQQPLAQNPSGKTEYMAFPKPFESSSSIGAEKQRNQKQPEEEVENSRTPWFYDQEGEVEKPFLKTGFAVSVEKTTNSNRKNQLDTSRRRRQFDEVSLESFNSMLDPVDPTTVTKTFKSRKASAQASLASKDKTPKSKSKKRHSTQLKSRVKNIGYESASVSSTCEPCKSRNRHSAQTEEPVQAKVLSRKNHEQLEKVIRCSRSTEISSAHARRILQQSNRNACNEAPETGSDFSMFEALRDTIYSEVATLISQNESRPHFLIELFHELQLLNTDYLRQRALYALQDIVSRHISENHEKEGENIKSVNSGTWIASNSELTPSESLATTDDETFEKNFERETHKISEQNDADNASVSSTCEPFATDVLGDTVIHLDQALARMRDYTHMKMEAESSPDVRCTCRIIEDDDGAAATTTISNNLEETPVIENHSSQQPVSEVATVPCPRIDTQQLDRQIKAIMKEVIPFLKEHMDEVCSSQLLTSVRRMVLVLTQQNNESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSVTVKQRCKRKIEAAGVIQSYAKEAKRILEGDHSSPAGEIDDEDKDKDETETVKQTQTSEVYDGDGPKNVRSDVSDQEDDEESEECPVSINLSKAETQALTNYGSGEDENEDEEIEEFEEGPVDVQTSLQANTETTEENEHDDQVPQHDFEKSSESKNVPSEQEPTTSKGDQNSTPVKPCYLSIVENEQPLNSPVQKDTLTTIDSSSQPNPLPLPLTEIETLVPRVKEVKSAQETPESSLAGSPDTESPVLVNDYEAESGNISQKSDEEDFVKVEDLPLKLTIYSEADLRKKMAEEEQKNHLSGEICAMQTEELAGNSQTLKEPGSHHIFSCLSLPPNVRPK

Target ORF information:

RefSeq Version	XM_006080139.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis pericentriolar material 1 (PCM1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006080139.1

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
AGCAATGAGA GTGTTGATGA CCGACTCAAC AATATGGATT GGGGCAGCCA GCAGAAGAAG 
GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA 
ACTAACGATA TTTCTCCGGA GTCATCACCG GGAGTTGGAA GACGAAGAAC AAAGACTTCA 
CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TCCCCGAGCA GGCAGAACTA 
GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGATTAATAC TAACAAGAGC 
AAAGATGCCA CTGTAAGTCC TCAAAAGAGA GAAATGATTG GATCAGCACA GTGTAAAGAG 
TTGTTTGCTT CTGCTTTAAG TAATGACCTT TTGCAAAACT GTCAAGTCTC TGAAGAAGAT 
GGAAGAGGAG AACCTGCAAT GGAGAGCAGC CAGATTGTAA GCAGGCTTGT TCAGATTCGT 
GAGTATATTA CTAAAGCTAG TTCCATGCGG GAAGACCTTG TAGAGAAAAA TGAAAGATCT 
GCTAATGTTG AGCGCCTCAC TCATCTAATA GATCACCTTA AAGAACAAGA AAAGTCATAT 
ATGAAATTTC TCCAGAAGAT TCTTGCTAGA GAAAATGAGG AGGAGGATGT TCGGACTATA 
GATTCAGCTG TGGGATCTGG TTCTGTAGCT GAGAGCACAT CGCTAAACAT AGATGTGCGG 
TCTGAGGCTT CAGATGCCAC GGAGGCATCT TTTAGTCTGA GAATTCGGCC GTGCGTTGAG 
GACAAACTAG GGAATTCAGC TTCACAAGCA CAGGTTTCAG ACACTGACGT TACTACAAGT 
CCAAAAGGGA AAGGTGACAG ACCTCCTCAG CATGACAGGG AGCTGAGGCC TAATAGGAAA 
TGTAGCCGAA AACGTGGATT TCCCTCAAAG GCCAGAGATC CTCAGCAGGA GCCTATGGAA 
GAGATAGAAA ATTTGAAGAA ACAACATGAT TTATTAAAAA GGATGTTACA GCAGCAGGAG 
CAGCTGAGAG CTCTACAAGG ACGACAAGCT GCTCTGCTTG CCCTGCAGCA TAAAGCTGAG 
CAGGCTATAG CTGTGATGGA TGATTCTGAA AAGGTTGCGG AGACAACTCC TGGCCATCAT 
ACTGTACCAG GCAGACAGCC GGCTCATTCA CCTTTGCATC AGAGAGTACC CATAAGAGAA 
ACTACAGGTA GCCTATCTGG AGTCAGTATC ACCTCTGAAC TAAACGAAGA ATTGAATGAT 
TTAATTCAGC ATTTTCATAA TCAGCTTCGT GATTCTCAGC CTCCAGCTGT TCCAGACAAT 
AGAAGACAAG CAGAAAGTCT TTCATTAACT AGGGAGATTT CTCAGAGCAG AAATCCTTCA 
GTTTCAGAGC ATTTACCTGA TGAGAAAGTC CAACTTTTTA GCAAGATGAG AGTGTTACAA 
GAAAAGAAAC AAAAAATGGA CAAACTTCTT GGAGAACTTC ATACACTTCG AGATCAGCAT 
TTGAACAATT CATCATTTGT GCCTTCTTCA GCCTCTCCAC AAAGGAGTGT GGATCAGAGA 
AGTACAACCA CAGCTCCTTC TGCTCCTGTA GTCTTAACAC CAGTGGTCAA TGGAGAATCC 
AATAACCTCA CGCCATCTGT TCCTTATCCT GCTGCTTCTC TAGTTTCTCA GAATCAGAAT 
GAAAATGAAG GCCACCTAAA TCCAACTGAA AAACTCCAGA AGTTAAATGA AGTTCGAAAG 
AGGTTGAATG AACTAAGAGA ATTAGTTCAT TATTATGAGC AAACATCAGA TATGATGACA 
GATGCTGTGA ATGAAAACAC AAAAGAAGAA GAAACTGAAG AATCAGAATA TGATTCGGAG 
CATGAAAACT CGGAACCTGT TACTAACATT CGAAATCCAC AAGTGGCTGC CACCTGGAAC 
GAAGTAAATA GTAATTCTAA TGCACAGTGT GTTTCCAACA ATAGAGATGG GCGAGCAGTT 
AATTCTAACT GTGAAATTAA CAACAGGTCT GCTGCCAACA TAAGGGCTCT AAACATGCCT 
CCTCTTTCAG CAGATTGTCG ATACAATAGA GAAGGAGAAC AGGAGATGCC TGTTGCACAA 
GGTCATGAGG AGGAGGAGGA GGAGGAGGAG GAAGCAGAAG ATGAGGCCCT CAGTGGAGCT 
TCACTGTCTA GTCACAGCAG CAGCCTGATC ATTGAGACTC CTGAAGATGC TGAATTTGAA 
CATAAGATCC AGAAGCTTAT GGCTGCAAAA CTGAAACTTA GACACTTACA GAACCTTGTA 
GCCATGGTGC AGGACGATGA TGCAGCCAAT CAAGGAGTTA CTTCTGTGGA GGATTTCTTC 
CCAGCAGAAG ACACCAAACA AAATGCAAAT AACACTAGAG GAAATGCCAA TAAAACACAG 
AAAGATGCTG GAGTAAATGA AAAGGCAAGG GAGAAATTTT ATGAGGCTAA ACTACAGCAG 
CAACAGAGAG AACTCAAACA ATTGCAGGAA GAAAGAAAGA AACTGATTGA GATTCAAGAG 
AAAATTCAAG AATTGCAAAA GGCATGTCCT AACCTACAGC TGTCAGCTGC TAGTGTGGGT 
AATTGTCCCA CAAAAACACA TATGCCAGCT GTTACTTCAT CCCCAACCGT TAATGAAAAT 
GAAGCCAGTA CAAACAAATC TGGTTTTGAG CCTCATGATT CTTCAGTGGT AGATAATGAG 
GTATGGTCAG ATATGCGAAG ACATGAAATG TTAAGGGAGG AGCTACGACA GAGAAGAAAG 
CAGCTGGAAG CTCTGATGGC TGAACATCAG AGGAGGCAAG GTCTGGCTGA AACTGCATCT 
CCAGTGGCTG TTTCACTGAG AAGTGATGGA TCTGAGAACC TGTGTACACC TCAGCAAAGC 
AGAACAGAAA AAACAATGGC AACTTGGGGA GGTTCTACCC AGTGTGCCCT AGATGAAGAA 
GGAGATGAAG ATGGTTACCT TTCTGAAGGA ATTATTCGGA CAGACGAAGA GGAGGAAGAG 
GAGGAGCAAG ATGCCAGTTC CAATGATAAC TTTCATATGT ATCCTCCAAA CAGTGCAAAT 
CATAATTCTT ACAATGTAAA GGAAACTAAA AATAGGTGGA AGAACAATCG CCCTTTTTCA 
GCAGATGGAA ATTATCGTCC TTTAGCCAAG ACAAGACAGC AGAATATCAG CATGCAGCGT 
CAGGAAAACC TTCGCTGGGT GTCCGAACTC TCTTATGTAG AAGAGAAAGA ACAATGGCAA 
GAACAAATTA ATCAGCTAAA GAAACAACTT GATTTCAGTG TCAATATTTG TCAGACTTTA 
ATGCAAGACC AGCAGACTCT GTCTTGTCTG CTACAAACTC TTCTCACGAG TCCTTATAGT 
GTTATGCCCA GCAGTGTTGC ATCCCCGCAA GTGCACCTTA TAATGCACCA GTTGAATCAG 
TGCTATACCC AGCTAACGTG GCAGCAGAAC AATGTCCAGA GGTTGAAACA AATGCTCAGT 
GAACTCATGC GCCAACAAAA TCAGCATCCA GAAAAACCTG CAAGCAAGGA AGGAGGCAGT 
GGTACATCAC ACCCTCCTTC TCCCAGTTTA TTTTGTCCTT TCAACTTTCC AACTCAGCCT 
GTAAATCTGT TTAACCTACC GGGGTTTACT AATTTTTCGT CATTTGCACC AAGTATGAAT 
TTCAGCCCTT TATTTCCTTC TAATTTTGGA GATTTTTCTC AAAATATTTC TACACCCACT 
GAACAGCAGC AACCGTTAGC CCAGAATCCT TCAGGGAAAA CGGAATACAT GGCTTTTCCA 
AAACCTTTTG AAAGTAGTTC TTCTATTGGA GCGGAAAAAC AAAGGAATCA AAAACAGCCT 
GAAGAGGAGG TGGAGAACAG TAGGACACCA TGGTTTTATG ACCAAGAAGG TGAAGTAGAA 
AAGCCATTTC TCAAGACTGG ATTTGCAGTG TCTGTAGAGA AAACAACAAA TAGTAATCGC 
AAAAATCAAC TAGATACCAG TAGGAGAAGA CGCCAGTTTG ATGAAGTGTC CTTAGAAAGC 
TTTAACAGTA TGCTTGACCC AGTAGATCCA ACAACAGTAA CTAAAACATT CAAGTCAAGA 
AAAGCATCTG CACAGGCCAG CCTGGCATCT AAAGATAAAA CTCCCAAATC AAAGAGTAAG 
AAGAGGCATT CTACTCAGCT GAAAAGCAGA GTGAAAAATA TCGGGTATGA AAGTGCCAGT 
GTGTCTAGCA CATGTGAACC TTGCAAAAGT AGAAACAGAC ATTCAGCCCA AACTGAAGAG 
CCTGTTCAAG CAAAAGTACT CAGCAGAAAG AATCATGAGC AGCTGGAAAA AGTCATAAGA 
TGTAGTAGGT CTACAGAAAT ATCTTCAGCA CATGCTAGGA GAATACTACA GCAGTCTAAC 
AGAAATGCAT GCAATGAAGC GCCAGAAACC GGGAGTGATT TTTCCATGTT CGAAGCTTTG 
CGGGATACCA TTTATTCTGA AGTAGCTACG TTGATTTCTC AAAATGAATC TCGTCCACAT 
TTTCTTATTG AACTCTTCCA TGAGCTTCAG CTACTTAATA CAGACTATTT GAGACAGAGG 
GCCTTATATG CATTGCAGGA CATAGTATCC AGGCATATTT CTGAGAACCA TGAAAAAGAA 
GGAGAAAATA TAAAGTCCGT GAATTCTGGT ACTTGGATAG CATCCAACTC AGAACTTACT 
CCCAGTGAAA GCCTTGCTAC TACTGATGAT GAAACTTTTG AGAAGAACTT TGAGAGAGAA 
ACACATAAAA TAAGTGAGCA AAATGATGCT GATAATGCTA GTGTATCTTC AACTTGTGAG 
CCTTTTGCAA CAGATGTTCT GGGCGACACT GTGATTCACT TAGATCAGGC ATTAGCCAGA 
ATGAGAGACT ATACGCATAT GAAGATGGAG GCTGAAAGTA GTCCAGACGT CAGGTGCACC 
TGCAGGATCA TTGAGGATGA CGATGGTGCT GCTGCCACAA CCACGATTAG TAATAATTTA 
GAAGAAACTC CTGTTATTGA AAATCATAGT TCACAGCAAC CCGTGAGTGA AGTTGCTACC 
GTCCCGTGTC CTAGAATTGA TACTCAGCAA CTGGACCGGC AAATTAAAGC AATTATGAAA 
GAAGTCATTC CTTTTTTGAA GGAGCACATG GATGAAGTAT GTTCTTCTCA ACTTCTAACC 
TCAGTAAGAC GCATGGTTTT GGTCCTTACT CAGCAAAACA ATGAGAGCAA AGAGTTTGTA 
AAGTTCTTTC ATAAGCAGCT TGGAAGTATA TTACAGGATT CACTCGCAAA ATTTGCTGGC 
AGAAAACTGA AGGATTGTGG AGAAGATCTT CTTGTAGAGA TATCTGAAGT GTTATTTAAT 
GAATTGGCTT TCTTTAAGCT CATGCAAGAT TTGGATAATA ATAGTGTAAC TGTTAAACAG 
AGATGCAAAA GGAAAATAGA AGCTGCTGGA GTGATACAGT CTTATGCAAA AGAGGCCAAA 
AGGATTCTTG AAGGAGATCA CAGTTCACCT GCTGGAGAAA TTGATGATGA AGACAAAGAC 
AAGGATGAGA CGGAAACAGT TAAGCAGACT CAAACGTCTG AGGTTTATGA TGGTGACGGC 
CCCAAAAATG TGAGATCTGA TGTTTCTGAT CAAGAGGATG ATGAAGAAAG TGAAGAATGT 
CCAGTGTCTA TTAATTTGTC CAAAGCTGAA ACTCAGGCTT TGACTAATTA CGGAAGTGGA 
GAAGATGAGA ATGAAGATGA AGAAATAGAA GAATTTGAAG AGGGACCTGT AGATGTCCAG 
ACGTCACTTC AGGCTAACAC TGAAACTACA GAAGAAAATG AACACGATGA TCAGGTTCCA 
CAGCATGATT TTGAAAAATC ATCAGAAAGC AAAAATGTCC CATCAGAACA AGAACCCACT 
ACTAGTAAAG GTGACCAGAA TAGCACTCCT GTGAAGCCCT GTTACCTCAG TATCGTGGAA 
AATGAGCAAC CTTTAAATAG CCCTGTCCAG AAGGACACTC TCACTACCAT CGACTCATCC 
AGTCAGCCTA ACCCTTTGCC TTTACCTTTA ACTGAAATTG AAACCTTGGT GCCTAGAGTC 
AAAGAAGTGA AATCTGCTCA GGAGACTCCT GAAAGTTCTT TGGCTGGAAG TCCTGATACT 
GAATCTCCAG TGTTAGTGAA TGACTATGAA GCAGAATCTG GTAACATAAG TCAAAAATCT 
GATGAAGAAG ACTTTGTGAA AGTTGAAGAT TTACCACTTA AACTGACAAT ATATTCAGAG 
GCAGATCTAA GGAAGAAAAT GGCCGAAGAA GAACAGAAAA ACCACTTATC TGGTGAAATA 
TGTGCAATGC AGACTGAAGA ATTAGCTGGG AATTCTCAGA CACTAAAAGA ACCTGGGTCC 
CACCACATTT TTAGTTGCCT ATCCCTGCCT CCAAATGTCA GACCAAAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( XM_006080140.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006080202.1 Bubalus bubalis pericentriolar material 1 (PCM1), transcript variant X7, mRNA.

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CloneID	OBu33067
Clone ID Related Accession (Same CDS sequence)	XM_006080140.1
Accession Version	XM_006080140.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6441bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	pericentriolar material 1 protein isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785878.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG GAGGAGGTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
AGCAATGAGA GTGTTGATGA CCGACTCAAC AATATGGATT GGGGCAGCCA GCAGAAGAAG 
GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA 
ACTAACGATA TTTCTCCGGA GTCATCACCG GGAGTTGGAA GACGAAGAAC AAAGACTTCA 
CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TCCCCGAGCA GGCAGAACTA 
GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGATTAATAC TAACAAGAGC 
AAAGATGCCA CTGTAAGTCC TCAAAAGAGA GAAATGATTG GATCAGCACA GTGTAAAGAG 
TTGTTTGCTT CTGCTTTAAG TAATGACCTT TTGCAAAACT GTCAAGTCTC TGAAGAAGAT 
GGAAGAGGAG AACCTGCAAT GGAGAGCAGC CAGATTGTAA GCAGGCTTGT TCAGATTCGT 
GAGTATATTA CTAAAGCTAG TTCCATGCGG GAAGACCTTG TAGAGAAAAA TGAAAGATCT 
GCTAATGTTG AGCGCCTCAC TCATCTAATA GATCACCTTA AAGAACAAGA AAAGTCATAT 
ATGAAATTTC TCCAGAAGAT TCTTGCTAGA GAAAATGAGG AGGAGGATGT TCGGACTATA 
GATTCAGCTG TGGGATCTGG TTCTGTAGCT GAGAGCACAT CGCTAAACAT AGATGTGCGG 
TCTGAGGCTT CAGATGCCAC GGAGGCATCT TTTAGTCTGA GAATTCGGCC GTGCGTTGAG 
GACAAACTAG GGAATTCAGC TTCACAAGCA CAGGTTTCAG ACACTGACGT TACTACAAGT 
CCAAAAGGGA AAGGTGACAG ACCTCCTCAG CATGACAGGG AGCTGAGGCC TAATAGGAAA 
TGTAGCCGAA AACGTGGATT TCCCTCAAAG GCCAGAGATC CTCAGCAGGA GCCTATGGAA 
GAGATAGAAA ATTTGAAGAA ACAACATGAT TTATTAAAAA GGATGTTACA GCAGCAGGAG 
CAGCTGAGAG CTCTACAAGG ACGACAAGCT GCTCTGCTTG CCCTGCAGCA TAAAGCTGAG 
CAGGCTATAG CTGTGATGGA TGATTCTGAA AAGGTTGCGG AGACAACTCC TGGCCATCAT 
ACTGTACCAG GCAGACAGCC GGCTCATTCA CCTTTGCATC AGAGAGTACC CATAAGAGAA 
ACTACAGGTA GCCTATCTGG AGTCAGTATC ACCTCTGAAC TAAACGAAGA ATTGAATGAT 
TTAATTCAGC ATTTTCATAA TCAGCTTCGT GATTCTCAGC CTCCAGCTGT TCCAGACAAT 
AGAAGACAAG CAGAAAGTCT TTCATTAACT AGGGAGATTT CTCAGAGCAG AAATCCTTCA 
GTTTCAGAGC ATTTACCTGA TGAGAAAGTC CAACTTTTTA GCAAGATGAG AGTGTTACAA 
GAAAAGAAAC AAAAAATGGA CAAACTTCTT GGAGAACTTC ATACACTTCG AGATCAGCAT 
TTGAACAATT CATCATTTGT GCCTTCTTCA GCCTCTCCAC AAAGGAGTGT GGATCAGAGA 
AGTACAACCA CAGCTCCTTC TGCTCCTGTA GTCTTAACAC CAGTGGTCAA TGGAGAATCC 
AATAACCTCA CGCCATCTGT TCCTTATCCT GCTGCTTCTC TAGTTTCTCA GAATCAGAAT 
GAAAATGAAG GCCACCTAAA TCCAACTGAA AAACTCCAGA AGTTAAATGA AGTTCGAAAG 
AGGTTGAATG AACTAAGAGA ATTAGTTCAT TATTATGAGC AAACATCAGA TATGATGACA 
GATGCTGTGA ATGAAAACAC AAAAGAAGAA GAAACTGAAG AATCAGAATA TGATTCGGAG 
CATGAAAACT CGGAACCTGT TACTAACATT CGAAATCCAC AAGTGGCTGC CACCTGGAAC 
GAAGTAAATA GTAATTCTAA TGCACAGTGT GTTTCCAACA ATAGAGATGG GCGAGCAGTT 
AATTCTAACT GTGAAATTAA CAACAGGTCT GCTGCCAACA TAAGGGCTCT AAACATGCCT 
CCTCTTTCAG CAGATTGTCG ATACAATAGA GAAGGAGAAC AGGAGATGCC TGTTGCACAA 
GGTCATGAGG AGGAGGAGGA GGAGGAGGAG GAAGCAGAAG ATGAGGCCCT CAGTGGAGCT 
TCACTGTCTA GTCACAGCAG CAGCCTGATC ATTGAGACTC CTGAAGATGC TGAATTTGAA 
CATAAGATCC AGAAGCTTAT GGCTGCAAAA CTGAAACTTA GACACTTACA GAACCTTGTA 
GCCATGGTGC AGGACGATGA TGCAGCCAAT CAAGGAGTTA CTTCTGTGGA GGATTTCTTC 
CCAGCAGAAG ACACCAAACA AAATGCAAAT AACACTAGAG GAAATGCCAA TAAAACACAG 
AAAGATGCTG GAGTAAATGA AAAGGCAAGG GAGAAATTTT ATGAGGCTAA ACTACAGCAG 
CAACAGAGAG AACTCAAACA ATTGCAGGAA GAAAGAAAGA AACTGATTGA GATTCAAGAG 
AAAATTCAAG AATTGCAAAA GGCATGTCCT AACCTACAGC TGTCAGCTGC TAGTGTGGGT 
AATTGTCCCA CAAAAACACA TATGCCAGCT GTTACTTCAT CCCCAACCGT TAATGAAAAT 
GAAGCCAGTA CAAACAAATC TGGTTTTGAG CCTCATGATT CTTCAGTGGT AGATAATGAG 
GTATGGTCAG ATATGCGAAG ACATGAAATG TTAAGGGAGG AGCTACGACA GAGAAGAAAG 
CAGCTGGAAG CTCTGATGGC TGAACATCAG AGGAGGCAAG GTCTGGCTGA AACTGCATCT 
CCAGTGGCTG TTTCACTGAG AAGTGATGGA TCTGAGAACC TGTGTACACC TCAGCAAAGC 
AGAACAGAAA AAACAATGGC AACTTGGGGA GGTTCTACCC AGTGTGCCCT AGATGAAGAA 
GGAGATGAAG ATGGTTACCT TTCTGAAGGA ATTATTCGGA CAGACGAAGA GGAGGAAGAG 
GAGGAGCAAG ATGCCAGTTC CAATGATAAC TTTCATATGT ATCCTCCAAA CAGTGCAAAT 
CATAATTCTT ACAATGTAAA GGAAACTAAA AATAGGTGGA AGAACAATCG CCCTTTTTCA 
GCAGATGGAA ATTATCGTCC TTTAGCCAAG ACAAGACAGC AGAATATCAG CATGCAGCGT 
CAGGAAAACC TTCGCTGGGT GTCCGAACTC TCTTATGTAG AAGAGAAAGA ACAATGGCAA 
GAACAAATTA ATCAGCTAAA GAAACAACTT GATTTCAGTG TCAATATTTG TCAGACTTTA 
ATGCAAGACC AGCAGACTCT GTCTTGTCTG CTACAAACTC TTCTCACGAG TCCTTATAGT 
GTTATGCCCA GCAGTGTTGC ATCCCCGCAA GTGCACCTTA TAATGCACCA GTTGAATCAG 
TGCTATACCC AGCTAACGTG GCAGCAGAAC AATGTCCAGA GGTTGAAACA AATGCTCAGT 
GAACTCATGC GCCAACAAAA TCAGCATCCA GAAAAACCTG CAAGCAAGGA AGGAGGCAGT 
GGTACATCAC ACCCTCCTTC TCCCAGTTTA TTTTGTCCTT TCAACTTTCC AACTCAGCCT 
GTAAATCTGT TTAACCTACC GGGGTTTACT AATTTTTCGT CATTTGCACC AAGTATGAAT 
TTCAGCCCTT TATTTCCTTC TAATTTTGGA GATTTTTCTC AAAATATTTC TACACCCACT 
GAACAGCAGC AACCGTTAGC CCAGAATCCT TCAGGGAAAA CGGAATACAT GGCTTTTCCA 
AAACCTTTTG AAAGTAGTTC TTCTATTGGA GCGGAAAAAC AAAGGAATCA AAAACAGCCT 
GAAGAGGAGG TGGAGAACAG TAGGACACCA TGGTTTTATG ACCAAGAAGG TGAAGTAGAA 
AAGCCATTTC TCAAGACTGG ATTTGCAGTG TCTGTAGAGA AAACAACAAA TAGTAATCGC 
AAAAATCAAC TAGATACCAG TAGGAGAAGA CGCCAGTTTG ATGAAGTGTC CTTAGAAAGC 
TTTAACAGTA TGCTTGACCC AGTAGATCCA ACAACAGTAA CTAAAACATT CAAGTCAAGA 
AAAGCATCTG CACAGGCCAG CCTGGCATCT AAAGATAAAA CTCCCAAATC AAAGAGTAAG 
AAGAGGCATT CTACTCAGCT GAAAAGCAGA GTGAAAAATA TCGGGTATGA AAGTGCCAGT 
GTGTCTAGCA CATGTGAACC TTGCAAAAGT AGAAACAGAC ATTCAGCCCA AACTGAAGAG 
CCTGTTCAAG CAAAAGTACT CAGCAGAAAG AATCATGAGC AGCTGGAAAA AGTCATAAGA 
TGTAGTAGGT CTACAGAAAT ATCTTCAGCA CATGCTAGGA GAATACTACA GCAGTCTAAC 
AGAAATGCAT GCAATGAAGC GCCAGAAACC GGGAGTGATT TTTCCATGTT CGAAGCTTTG 
CGGGATACCA TTTATTCTGA AGTAGCTACG TTGATTTCTC AAAATGAATC TCGTCCACAT 
TTTCTTATTG AACTCTTCCA TGAGCTTCAG CTACTTAATA CAGACTATTT GAGACAGAGG 
GCCTTATATG CATTGCAGGA CATAGTATCC AGGCATATTT CTGAGAACCA TGAAAAAGAA 
GGAGAAAATA TAAAGTCCGT GAATTCTGGT ACTTGGATAG CATCCAACTC AGAACTTACT 
CCCAGTGAAA GCCTTGCTAC TACTGATGAT GAAACTTTTG AGAAGAACTT TGAGAGAGAA 
ACACATAAAA TAAGTGAGCA AAATGATGCT GATAATGCTA GTGTATCTTC AACTTGTGAG 
CCTTTTGCAA CAGATGTTCT GGGCGACACT GTGATTCACT TAGATCAGGC ATTAGCCAGA 
ATGAGAGACT ATACGCATAT GAAGATGGAG GCTGAAAGTA GTCCAGACGT CAGGTGCACC 
TGCAGGATCA TTGAGGATGA CGATGGTGCT GCTGCCACAA CCACGATTAG TAATAATTTA 
GAAGAAACTC CTGTTATTGA AAATCATAGT TCACAGCAAC CCGTGAGTGA AGTTGCTACC 
GTCCCGTGTC CTAGAATTGA TACTCAGCAA CTGGACCGGC AAATTAAAGC AATTATGAAA 
GAAGTCATTC CTTTTTTGAA GGAGCACATG GATGAAGTAT GTTCTTCTCA ACTTCTAACC 
TCAGTAAGAC GCATGGTTTT GGTCCTTACT CAGCAAAACA ATGAGAGCAA AGAGTTTGTA 
AAGTTCTTTC ATAAGCAGCT TGGAAGTATA TTACAGGATT CACTCGCAAA ATTTGCTGGC 
AGAAAACTGA AGGATTGTGG AGAAGATCTT CTTGTAGAGA TATCTGAAGT GTTATTTAAT 
GAATTGGCTT TCTTTAAGCT CATGCAAGAT TTGGATAATA ATAGTGTAAC TGTTAAACAG 
AGATGCAAAA GGAAAATAGA AGCTGCTGGA GTGATACAGT CTTATGCAAA AGAGGCCAAA 
AGGATTCTTG AAGGAGATCA CAGTTCACCT GCTGGAGAAA TTGATGATGA AGACAAAGAC 
AAGGATGAGA CGGAAACAGT TAAGCAGACT CAAACGTCTG AGGTTTATGA TGGTGACGGC 
CCCAAAAATG TGAGATCTGA TGTTTCTGAT CAAGAGGATG ATGAAGAAAG TGAAGAATGT 
CCAGTGTCTA TTAATTTGTC CAAAGCTGAA ACTCAGGCTT TGACTAATTA CGGAAGTGGA 
GAAGATGAGA ATGAAGATGA AGAAATAGAA GAATTTGAAG AGGGACCTGT AGATGTCCAG 
ACGTCACTTC AGGCTAACAC TGAAACTACA GAAGAAAATG AACACGATGA TCAGGTTCCA 
CAGCATGATT TTGAAAAATC ATCAGAAAGC AAAAATGTCC CATCAGAACA AGAACCCACT 
ACTAGTAAAG GTGACCAGAA TAGCACTCCT GTGAAGCCCT GTTACCTCAG TATCGTGGAA 
AATGAGCAAC CTTTAAATAG CCCTGTCCAG AAGGACACTC TCACTACCAT CGACTCATCC 
AGTCAGCCTA ACCCTTTGCC TTTACCTTTA ACTGAAATTG AAACCTTGGT GCCTAGAGTC 
AAAGAAGTGA AATCTGCTCA GGAGACTCCT GAAAGTTCTT TGGCTGGAAG TCCTGATACT 
GAATCTCCAG TGTTAGTGAA TGACTATGAA GCAGAATCTG GTAACATAAG TCAAAAATCT 
GATGAAGAAG ACTTTGTGAA AGTTGAAGAT TTACCACTTA AACTGACAAT ATATTCAGAG 
GCAGATCTAA GGAAGAAAAT GGCCGAAGAA GAACAGAAAA ACCACTTATC TGGTGAAATA 
TGTGCAATGC AGACTGAAGA ATTAGCTGGG AATTCTCAGA CACTAAAAGA ACCTGAAACA 
GTGGGAGCCC AAAGTACATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006080202.1
CDS	113..6553
Translation	MATGGGPFEEGVNDQDLPNWSNESVDDRLNNMDWGSQQKKANRSSEKNKRKFGVESDKRVTNDISPESSPGVGRRRTKTSLTFPHSRYMTQMSVPEQAELEKLKQRINFSDLDQINTNKSKDATVSPQKREMIGSAQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIREYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLQKILARENEEEDVRTIDSAVGSGSVAESTSLNIDVRSEASDATEASFSLRIRPCVEDKLGNSASQAQVSDTDVTTSPKGKGDRPPQHDRELRPNRKCSRKRGFPSKARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSEKVAETTPGHHTVPGRQPAHSPLHQRVPIRETTGSLSGVSITSELNEELNDLIQHFHNQLRDSQPPAVPDNRRQAESLSLTREISQSRNPSVSEHLPDEKVQLFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSFVPSSASPQRSVDQRSTTTAPSAPVVLTPVVNGESNNLTPSVPYPAASLVSQNQNENEGHLNPTEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENTKEEETEESEYDSEHENSEPVTNIRNPQVAATWNEVNSNSNAQCVSNNRDGRAVNSNCEINNRSAANIRALNMPPLSADCRYNREGEQEMPVAQGHEEEEEEEEEAEDEALSGASLSSHSSSLIIETPEDAEFEHKIQKLMAAKLKLRHLQNLVAMVQDDDAANQGVTSVEDFFPAEDTKQNANNTRGNANKTQKDAGVNEKAREKFYEAKLQQQQRELKQLQEERKKLIEIQEKIQELQKACPNLQLSAASVGNCPTKTHMPAVTSSPTVNENEASTNKSGFEPHDSSVVDNEVWSDMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETASPVAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGIIRTDEEEEEEEQDASSNDNFHMYPPNSANHNSYNVKETKNRWKNNRPFSADGNYRPLAKTRQQNISMQRQENLRWVSELSYVEEKEQWQEQINQLKKQLDFSVNICQTLMQDQQTLSCLLQTLLTSPYSVMPSSVASPQVHLIMHQLNQCYTQLTWQQNNVQRLKQMLSELMRQQNQHPEKPASKEGGSGTSHPPSPSLFCPFNFPTQPVNLFNLPGFTNFSSFAPSMNFSPLFPSNFGDFSQNISTPTEQQQPLAQNPSGKTEYMAFPKPFESSSSIGAEKQRNQKQPEEEVENSRTPWFYDQEGEVEKPFLKTGFAVSVEKTTNSNRKNQLDTSRRRRQFDEVSLESFNSMLDPVDPTTVTKTFKSRKASAQASLASKDKTPKSKSKKRHSTQLKSRVKNIGYESASVSSTCEPCKSRNRHSAQTEEPVQAKVLSRKNHEQLEKVIRCSRSTEISSAHARRILQQSNRNACNEAPETGSDFSMFEALRDTIYSEVATLISQNESRPHFLIELFHELQLLNTDYLRQRALYALQDIVSRHISENHEKEGENIKSVNSGTWIASNSELTPSESLATTDDETFEKNFERETHKISEQNDADNASVSSTCEPFATDVLGDTVIHLDQALARMRDYTHMKMEAESSPDVRCTCRIIEDDDGAAATTTISNNLEETPVIENHSSQQPVSEVATVPCPRIDTQQLDRQIKAIMKEVIPFLKEHMDEVCSSQLLTSVRRMVLVLTQQNNESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSVTVKQRCKRKIEAAGVIQSYAKEAKRILEGDHSSPAGEIDDEDKDKDETETVKQTQTSEVYDGDGPKNVRSDVSDQEDDEESEECPVSINLSKAETQALTNYGSGEDENEDEEIEEFEEGPVDVQTSLQANTETTEENEHDDQVPQHDFEKSSESKNVPSEQEPTTSKGDQNSTPVKPCYLSIVENEQPLNSPVQKDTLTTIDSSSQPNPLPLPLTEIETLVPRVKEVKSAQETPESSLAGSPDTESPVLVNDYEAESGNISQKSDEEDFVKVEDLPLKLTIYSEADLRKKMAEEEQKNHLSGEICAMQTEELAGNSQTLKEPETVGAQST

Target ORF information:

RefSeq Version	XM_006080140.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis pericentriolar material 1 (PCM1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006080140.1

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
AGCAATGAGA GTGTTGATGA CCGACTCAAC AATATGGATT GGGGCAGCCA GCAGAAGAAG 
GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA 
ACTAACGATA TTTCTCCGGA GTCATCACCG GGAGTTGGAA GACGAAGAAC AAAGACTTCA 
CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TCCCCGAGCA GGCAGAACTA 
GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGATTAATAC TAACAAGAGC 
AAAGATGCCA CTGTAAGTCC TCAAAAGAGA GAAATGATTG GATCAGCACA GTGTAAAGAG 
TTGTTTGCTT CTGCTTTAAG TAATGACCTT TTGCAAAACT GTCAAGTCTC TGAAGAAGAT 
GGAAGAGGAG AACCTGCAAT GGAGAGCAGC CAGATTGTAA GCAGGCTTGT TCAGATTCGT 
GAGTATATTA CTAAAGCTAG TTCCATGCGG GAAGACCTTG TAGAGAAAAA TGAAAGATCT 
GCTAATGTTG AGCGCCTCAC TCATCTAATA GATCACCTTA AAGAACAAGA AAAGTCATAT 
ATGAAATTTC TCCAGAAGAT TCTTGCTAGA GAAAATGAGG AGGAGGATGT TCGGACTATA 
GATTCAGCTG TGGGATCTGG TTCTGTAGCT GAGAGCACAT CGCTAAACAT AGATGTGCGG 
TCTGAGGCTT CAGATGCCAC GGAGGCATCT TTTAGTCTGA GAATTCGGCC GTGCGTTGAG 
GACAAACTAG GGAATTCAGC TTCACAAGCA CAGGTTTCAG ACACTGACGT TACTACAAGT 
CCAAAAGGGA AAGGTGACAG ACCTCCTCAG CATGACAGGG AGCTGAGGCC TAATAGGAAA 
TGTAGCCGAA AACGTGGATT TCCCTCAAAG GCCAGAGATC CTCAGCAGGA GCCTATGGAA 
GAGATAGAAA ATTTGAAGAA ACAACATGAT TTATTAAAAA GGATGTTACA GCAGCAGGAG 
CAGCTGAGAG CTCTACAAGG ACGACAAGCT GCTCTGCTTG CCCTGCAGCA TAAAGCTGAG 
CAGGCTATAG CTGTGATGGA TGATTCTGAA AAGGTTGCGG AGACAACTCC TGGCCATCAT 
ACTGTACCAG GCAGACAGCC GGCTCATTCA CCTTTGCATC AGAGAGTACC CATAAGAGAA 
ACTACAGGTA GCCTATCTGG AGTCAGTATC ACCTCTGAAC TAAACGAAGA ATTGAATGAT 
TTAATTCAGC ATTTTCATAA TCAGCTTCGT GATTCTCAGC CTCCAGCTGT TCCAGACAAT 
AGAAGACAAG CAGAAAGTCT TTCATTAACT AGGGAGATTT CTCAGAGCAG AAATCCTTCA 
GTTTCAGAGC ATTTACCTGA TGAGAAAGTC CAACTTTTTA GCAAGATGAG AGTGTTACAA 
GAAAAGAAAC AAAAAATGGA CAAACTTCTT GGAGAACTTC ATACACTTCG AGATCAGCAT 
TTGAACAATT CATCATTTGT GCCTTCTTCA GCCTCTCCAC AAAGGAGTGT GGATCAGAGA 
AGTACAACCA CAGCTCCTTC TGCTCCTGTA GTCTTAACAC CAGTGGTCAA TGGAGAATCC 
AATAACCTCA CGCCATCTGT TCCTTATCCT GCTGCTTCTC TAGTTTCTCA GAATCAGAAT 
GAAAATGAAG GCCACCTAAA TCCAACTGAA AAACTCCAGA AGTTAAATGA AGTTCGAAAG 
AGGTTGAATG AACTAAGAGA ATTAGTTCAT TATTATGAGC AAACATCAGA TATGATGACA 
GATGCTGTGA ATGAAAACAC AAAAGAAGAA GAAACTGAAG AATCAGAATA TGATTCGGAG 
CATGAAAACT CGGAACCTGT TACTAACATT CGAAATCCAC AAGTGGCTGC CACCTGGAAC 
GAAGTAAATA GTAATTCTAA TGCACAGTGT GTTTCCAACA ATAGAGATGG GCGAGCAGTT 
AATTCTAACT GTGAAATTAA CAACAGGTCT GCTGCCAACA TAAGGGCTCT AAACATGCCT 
CCTCTTTCAG CAGATTGTCG ATACAATAGA GAAGGAGAAC AGGAGATGCC TGTTGCACAA 
GGTCATGAGG AGGAGGAGGA GGAGGAGGAG GAAGCAGAAG ATGAGGCCCT CAGTGGAGCT 
TCACTGTCTA GTCACAGCAG CAGCCTGATC ATTGAGACTC CTGAAGATGC TGAATTTGAA 
CATAAGATCC AGAAGCTTAT GGCTGCAAAA CTGAAACTTA GACACTTACA GAACCTTGTA 
GCCATGGTGC AGGACGATGA TGCAGCCAAT CAAGGAGTTA CTTCTGTGGA GGATTTCTTC 
CCAGCAGAAG ACACCAAACA AAATGCAAAT AACACTAGAG GAAATGCCAA TAAAACACAG 
AAAGATGCTG GAGTAAATGA AAAGGCAAGG GAGAAATTTT ATGAGGCTAA ACTACAGCAG 
CAACAGAGAG AACTCAAACA ATTGCAGGAA GAAAGAAAGA AACTGATTGA GATTCAAGAG 
AAAATTCAAG AATTGCAAAA GGCATGTCCT AACCTACAGC TGTCAGCTGC TAGTGTGGGT 
AATTGTCCCA CAAAAACACA TATGCCAGCT GTTACTTCAT CCCCAACCGT TAATGAAAAT 
GAAGCCAGTA CAAACAAATC TGGTTTTGAG CCTCATGATT CTTCAGTGGT AGATAATGAG 
GTATGGTCAG ATATGCGAAG ACATGAAATG TTAAGGGAGG AGCTACGACA GAGAAGAAAG 
CAGCTGGAAG CTCTGATGGC TGAACATCAG AGGAGGCAAG GTCTGGCTGA AACTGCATCT 
CCAGTGGCTG TTTCACTGAG AAGTGATGGA TCTGAGAACC TGTGTACACC TCAGCAAAGC 
AGAACAGAAA AAACAATGGC AACTTGGGGA GGTTCTACCC AGTGTGCCCT AGATGAAGAA 
GGAGATGAAG ATGGTTACCT TTCTGAAGGA ATTATTCGGA CAGACGAAGA GGAGGAAGAG 
GAGGAGCAAG ATGCCAGTTC CAATGATAAC TTTCATATGT ATCCTCCAAA CAGTGCAAAT 
CATAATTCTT ACAATGTAAA GGAAACTAAA AATAGGTGGA AGAACAATCG CCCTTTTTCA 
GCAGATGGAA ATTATCGTCC TTTAGCCAAG ACAAGACAGC AGAATATCAG CATGCAGCGT 
CAGGAAAACC TTCGCTGGGT GTCCGAACTC TCTTATGTAG AAGAGAAAGA ACAATGGCAA 
GAACAAATTA ATCAGCTAAA GAAACAACTT GATTTCAGTG TCAATATTTG TCAGACTTTA 
ATGCAAGACC AGCAGACTCT GTCTTGTCTG CTACAAACTC TTCTCACGAG TCCTTATAGT 
GTTATGCCCA GCAGTGTTGC ATCCCCGCAA GTGCACCTTA TAATGCACCA GTTGAATCAG 
TGCTATACCC AGCTAACGTG GCAGCAGAAC AATGTCCAGA GGTTGAAACA AATGCTCAGT 
GAACTCATGC GCCAACAAAA TCAGCATCCA GAAAAACCTG CAAGCAAGGA AGGAGGCAGT 
GGTACATCAC ACCCTCCTTC TCCCAGTTTA TTTTGTCCTT TCAACTTTCC AACTCAGCCT 
GTAAATCTGT TTAACCTACC GGGGTTTACT AATTTTTCGT CATTTGCACC AAGTATGAAT 
TTCAGCCCTT TATTTCCTTC TAATTTTGGA GATTTTTCTC AAAATATTTC TACACCCACT 
GAACAGCAGC AACCGTTAGC CCAGAATCCT TCAGGGAAAA CGGAATACAT GGCTTTTCCA 
AAACCTTTTG AAAGTAGTTC TTCTATTGGA GCGGAAAAAC AAAGGAATCA AAAACAGCCT 
GAAGAGGAGG TGGAGAACAG TAGGACACCA TGGTTTTATG ACCAAGAAGG TGAAGTAGAA 
AAGCCATTTC TCAAGACTGG ATTTGCAGTG TCTGTAGAGA AAACAACAAA TAGTAATCGC 
AAAAATCAAC TAGATACCAG TAGGAGAAGA CGCCAGTTTG ATGAAGTGTC CTTAGAAAGC 
TTTAACAGTA TGCTTGACCC AGTAGATCCA ACAACAGTAA CTAAAACATT CAAGTCAAGA 
AAAGCATCTG CACAGGCCAG CCTGGCATCT AAAGATAAAA CTCCCAAATC AAAGAGTAAG 
AAGAGGCATT CTACTCAGCT GAAAAGCAGA GTGAAAAATA TCGGGTATGA AAGTGCCAGT 
GTGTCTAGCA CATGTGAACC TTGCAAAAGT AGAAACAGAC ATTCAGCCCA AACTGAAGAG 
CCTGTTCAAG CAAAAGTACT CAGCAGAAAG AATCATGAGC AGCTGGAAAA AGTCATAAGA 
TGTAGTAGGT CTACAGAAAT ATCTTCAGCA CATGCTAGGA GAATACTACA GCAGTCTAAC 
AGAAATGCAT GCAATGAAGC GCCAGAAACC GGGAGTGATT TTTCCATGTT CGAAGCTTTG 
CGGGATACCA TTTATTCTGA AGTAGCTACG TTGATTTCTC AAAATGAATC TCGTCCACAT 
TTTCTTATTG AACTCTTCCA TGAGCTTCAG CTACTTAATA CAGACTATTT GAGACAGAGG 
GCCTTATATG CATTGCAGGA CATAGTATCC AGGCATATTT CTGAGAACCA TGAAAAAGAA 
GGAGAAAATA TAAAGTCCGT GAATTCTGGT ACTTGGATAG CATCCAACTC AGAACTTACT 
CCCAGTGAAA GCCTTGCTAC TACTGATGAT GAAACTTTTG AGAAGAACTT TGAGAGAGAA 
ACACATAAAA TAAGTGAGCA AAATGATGCT GATAATGCTA GTGTATCTTC AACTTGTGAG 
CCTTTTGCAA CAGATGTTCT GGGCGACACT GTGATTCACT TAGATCAGGC ATTAGCCAGA 
ATGAGAGACT ATACGCATAT GAAGATGGAG GCTGAAAGTA GTCCAGACGT CAGGTGCACC 
TGCAGGATCA TTGAGGATGA CGATGGTGCT GCTGCCACAA CCACGATTAG TAATAATTTA 
GAAGAAACTC CTGTTATTGA AAATCATAGT TCACAGCAAC CCGTGAGTGA AGTTGCTACC 
GTCCCGTGTC CTAGAATTGA TACTCAGCAA CTGGACCGGC AAATTAAAGC AATTATGAAA 
GAAGTCATTC CTTTTTTGAA GGAGCACATG GATGAAGTAT GTTCTTCTCA ACTTCTAACC 
TCAGTAAGAC GCATGGTTTT GGTCCTTACT CAGCAAAACA ATGAGAGCAA AGAGTTTGTA 
AAGTTCTTTC ATAAGCAGCT TGGAAGTATA TTACAGGATT CACTCGCAAA ATTTGCTGGC 
AGAAAACTGA AGGATTGTGG AGAAGATCTT CTTGTAGAGA TATCTGAAGT GTTATTTAAT 
GAATTGGCTT TCTTTAAGCT CATGCAAGAT TTGGATAATA ATAGTGTAAC TGTTAAACAG 
AGATGCAAAA GGAAAATAGA AGCTGCTGGA GTGATACAGT CTTATGCAAA AGAGGCCAAA 
AGGATTCTTG AAGGAGATCA CAGTTCACCT GCTGGAGAAA TTGATGATGA AGACAAAGAC 
AAGGATGAGA CGGAAACAGT TAAGCAGACT CAAACGTCTG AGGTTTATGA TGGTGACGGC 
CCCAAAAATG TGAGATCTGA TGTTTCTGAT CAAGAGGATG ATGAAGAAAG TGAAGAATGT 
CCAGTGTCTA TTAATTTGTC CAAAGCTGAA ACTCAGGCTT TGACTAATTA CGGAAGTGGA 
GAAGATGAGA ATGAAGATGA AGAAATAGAA GAATTTGAAG AGGGACCTGT AGATGTCCAG 
ACGTCACTTC AGGCTAACAC TGAAACTACA GAAGAAAATG AACACGATGA TCAGGTTCCA 
CAGCATGATT TTGAAAAATC ATCAGAAAGC AAAAATGTCC CATCAGAACA AGAACCCACT 
ACTAGTAAAG GTGACCAGAA TAGCACTCCT GTGAAGCCCT GTTACCTCAG TATCGTGGAA 
AATGAGCAAC CTTTAAATAG CCCTGTCCAG AAGGACACTC TCACTACCAT CGACTCATCC 
AGTCAGCCTA ACCCTTTGCC TTTACCTTTA ACTGAAATTG AAACCTTGGT GCCTAGAGTC 
AAAGAAGTGA AATCTGCTCA GGAGACTCCT GAAAGTTCTT TGGCTGGAAG TCCTGATACT 
GAATCTCCAG TGTTAGTGAA TGACTATGAA GCAGAATCTG GTAACATAAG TCAAAAATCT 
GATGAAGAAG ACTTTGTGAA AGTTGAAGAT TTACCACTTA AACTGACAAT ATATTCAGAG 
GCAGATCTAA GGAAGAAAAT GGCCGAAGAA GAACAGAAAA ACCACTTATC TGGTGAAATA 
TGTGCAATGC AGACTGAAGA ATTAGCTGGG AATTCTCAGA CACTAAAAGA ACCTGAAACA 
GTGGGAGCCC AAAGTACATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( XM_006080141.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006080203.1 Bubalus bubalis pericentriolar material 1 (PCM1), transcript variant X8, mRNA.

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CloneID	OBu33068
Clone ID Related Accession (Same CDS sequence)	XM_006080141.1
Accession Version	XM_006080141.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6387bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	pericentriolar material 1 protein isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785878.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG GAGGAGGTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
AGCAATGAGA GTGTTGATGA CCGACTCAAC AATATGGATT GGGGCAGCCA GCAGAAGAAG 
GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA 
ACTAACGATA TTTCTCCGGA GTCATCACCG GGAGTTGGAA GACGAAGAAC AAAGACTTCA 
CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TCCCCGAGCA GGCAGAACTA 
GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGATTAATAC TAACAAGAGC 
AAAGATGCCA CTGTAAGTCC TCAAAAGAGA GAAATGATTG GATCAGCACA GTGTAAAGAG 
TTGTTTGCTT CTGCTTTAAG TAATGACCTT TTGCAAAACT GTCAAGTCTC TGAAGAAGAT 
GGAAGAGGAG AACCTGCAAT GGAGAGCAGC CAGATTGTAA GCAGGCTTGT TCAGATTCGT 
GAGTATATTA CTAAAGCTAG TTCCATGCGG GAAGACCTTG TAGAGAAAAA TGAAAGATCT 
GCTAATGTTG AGCGCCTCAC TCATCTAATA GATCACCTTA AAGAACAAGA AAAGTCATAT 
ATGAAATTTC TCCAGAAGAT TCTTGCTAGA GAAAATGAGG AGGAGGATGT TCGGACTATA 
GATTCAGCTG TGGGATCTGG TTCTGTAGCT GAGAGCACAT CGCTAAACAT AGATGTGCGG 
TCTGAGGCTT CAGATGCCAC GGCCAGAGAT CCTCAGCAGG AGCCTATGGA AGAGATAGAA 
AATTTGAAGA AACAACATGA TTTATTAAAA AGGATGTTAC AGCAGCAGGA GCAGCTGAGA 
GCTCTACAAG GACGACAAGC TGCTCTGCTT GCCCTGCAGC ATAAAGCTGA GCAGGCTATA 
GCTGTGATGG ATGATTCTGA AAAGGTTGCG GAGACAACTC CTGGCCATCA TACTGTACCA 
GGCAGACAGC CGGCTCATTC ACCTTTGCAT CAGAGAGTAC CCATAAGAGA AACTACAGGT 
AGCCTATCTG GAGTCAGTAT CACCTCTGAA CTAAACGAAG AATTGAATGA TTTAATTCAG 
CATTTTCATA ATCAGCTTCG TGATTCTCAG CCTCCAGCTG TTCCAGACAA TAGAAGACAA 
GCAGAAAGTC TTTCATTAAC TAGGGAGATT TCTCAGAGCA GAAATCCTTC AGTTTCAGAG 
CATTTACCTG ATGAGAAAGT CCAACTTTTT AGCAAGATGA GAGTGTTACA AGAAAAGAAA 
CAAAAAATGG ACAAACTTCT TGGAGAACTT CATACACTTC GAGATCAGCA TTTGAACAAT 
TCATCATTTG TGCCTTCTTC AGCCTCTCCA CAAAGGAGTG TGGATCAGAG AAGTACAACC 
ACAGCTCCTT CTGCTCCTGT AGTCTTAACA CCAGTGGTCA ATGGAGAATC CAATAACCTC 
ACGCCATCTG TTCCTTATCC TGCTGCTTCT CTAGTTTCTC AGAATCAGAA TGAAAATGAA 
GGCCACCTAA ATCCAACTGA AAAACTCCAG AAGTTAAATG AAGTTCGAAA GAGGTTGAAT 
GAACTAAGAG AATTAGTTCA TTATTATGAG CAAACATCAG ATATGATGAC AGATGCTGTG 
AATGAAAACA CAAAAGAAGA AGAAACTGAA GAATCAGAAT ATGATTCGGA GCATGAAAAC 
TCGGAACCTG TTACTAACAT TCGAAATCCA CAAGTGGCTG CCACCTGGAA CGAAGTAAAT 
AGTAATTCTA ATGCACAGTG TGTTTCCAAC AATAGAGATG GGCGAGCAGT TAATTCTAAC 
TGTGAAATTA ACAACAGGTC TGCTGCCAAC ATAAGGGCTC TAAACATGCC TCCTCTTTCA 
GCAGATTGTC GATACAATAG AGAAGGAGAA CAGGAGATGC CTGTTGCACA AGGTCATGAG 
GAGGAGGAGG AGGAGGAGGA GGAAGCAGAA GATGAGGCCC TCAGTGGAGC TTCACTGTCT 
AGTCACAGCA GCAGCCTGAT CATTGAGACT CCTGAAGATG CTGAATTTGA ACATAAGATC 
CAGAAGCTTA TGGCTGCAAA ACTGAAACTT AGACACTTAC AGAACCTTGT AGCCATGGTG 
CAGGACGATG ATGCAGCCAA TCAAGGAGTT ACTTCTGTGG AGGATTTCTT CCCAGCAGAA 
GACACCAAAC AAAATGCAAA TAACACTAGA GGAAATGCCA ATAAAACACA GAAAGATGCT 
GGAGTAAATG AAAAGGCAAG GGAGAAATTT TATGAGGCTA AACTACAGCA GCAACAGAGA 
GAACTCAAAC AATTGCAGGA AGAAAGAAAG AAACTGATTG AGATTCAAGA GAAAATTCAA 
GAATTGCAAA AGGCATGTCC TAACCTACAG CTGTCAGCTG CTAGTGTGGG TAATTGTCCC 
ACAAAAACAC ATATGCCAGC TGTTACTTCA TCCCCAACCG TTAATGAAAA TGAAGCCAGT 
ACAAACAAAT CTGGTTTTGA GCCTCATGAT TCTTCAGTGG TAGATAATGA GGTATGGTCA 
GATATGCGAA GACATGAAAT GTTAAGGGAG GAGCTACGAC AGAGAAGAAA GCAGCTGGAA 
GCTCTGATGG CTGAACATCA GAGGAGGCAA GGTCTGGCTG AAACTGCATC TCCAGTGGCT 
GTTTCACTGA GAAGTGATGG ATCTGAGAAC CTGTGTACAC CTCAGCAAAG CAGAACAGAA 
AAAACAATGG CAACTTGGGG AGGTTCTACC CAGTGTGCCC TAGATGAAGA AGGAGATGAA 
GATGGTTACC TTTCTGAAGG AATTATTCGG ACAGACGAAG AGGAGGAAGA GGAGGAGCAA 
GATGCCAGTT CCAATGATAA CTTTCATATG TATCCTCCAA ACAGTGCAAA TCATAATTCT 
TACAATGTAA AGGAAACTAA AAATAGGTGG AAGAACAATC GCCCTTTTTC AGCAGATGGA 
AATTATCGTC CTTTAGCCAA GACAAGACAG CAGAATATCA GCATGCAGCG TCAGGAAAAC 
CTTCGCTGGG TGTCCGAACT CTCTTATGTA GAAGAGAAAG AACAATGGCA AGAACAAATT 
AATCAGCTAA AGAAACAACT TGATTTCAGT GTCAATATTT GTCAGACTTT AATGCAAGAC 
CAGCAGACTC TGTCTTGTCT GCTACAAACT CTTCTCACGA GTCCTTATAG TGTTATGCCC 
AGCAGTGTTG CATCCCCGCA AGTGCACCTT ATAATGCACC AGTTGAATCA GTGCTATACC 
CAGCTAACGT GGCAGCAGAA CAATGTCCAG AGGTTGAAAC AAATGCTCAG TGAACTCATG 
CGCCAACAAA ATCAGCATCC AGAAAAACCT GCAAGCAAGG AAGGAGGCAG TGGTACATCA 
CACCCTCCTT CTCCCAGTTT ATTTTGTCCT TTCAACTTTC CAACTCAGCC TGTAAATCTG 
TTTAACCTAC CGGGGTTTAC TAATTTTTCG TCATTTGCAC CAAGTATGAA TTTCAGCCCT 
TTATTTCCTT CTAATTTTGG AGATTTTTCT CAAAATATTT CTACACCCAC TGAACAGCAG 
CAACCGTTAG CCCAGAATCC TTCAGGGAAA ACGGAATACA TGGCTTTTCC AAAACCTTTT 
GAAAGTAGTT CTTCTATTGG AGCGGAAAAA CAAAGGAATC AAAAACAGCC TGAAGAGGAG 
GTGGAGAACA GTAGGACACC ATGGTTTTAT GACCAAGAAG GTGAAGTAGA AAAGCCATTT 
CTCAAGACTG GATTTGCAGT GTCTGTAGAG AAAACAACAA ATAGTAATCG CAAAAATCAA 
CTAGATACCA GTAGGAGAAG ACGCCAGTTT GATGAAGTGT CCTTAGAAAG CTTTAACAGT 
ATGCTTGACC CAGTAGATCC AACAACAGTA ACTAAAACAT TCAAGTCAAG AAAAGCATCT 
GCACAGGCCA GCCTGGCATC TAAAGATAAA ACTCCCAAAT CAAAGAGTAA GAAGAGGCAT 
TCTACTCAGC TGAAAAGCAG AGTGAAAAAT ATCGGGTATG AAAGTGCCAG TGTGTCTAGC 
ACATGTGAAC CTTGCAAAAG TAGAAACAGA CATTCAGCCC AAACTGAAGA GCCTGTTCAA 
GCAAAAGTAC TCAGCAGAAA GAATCATGAG CAGCTGGAAA AAGTCATAAG ATGTAGTAGG 
TCTACAGAAA TATCTTCAGC ACATGCTAGG AGAATACTAC AGCAGTCTAA CAGAAATGCA 
TGCAATGAAG CGCCAGAAAC CGGGAGTGAT TTTTCCATGT TCGAAGCTTT GCGGGATACC 
ATTTATTCTG AAGTAGCTAC GTTGATTTCT CAAAATGAAT CTCGTCCACA TTTTCTTATT 
GAACTCTTCC ATGAGCTTCA GCTACTTAAT ACAGACTATT TGAGACAGAG GGCCTTATAT 
GCATTGCAGG ACATAGTATC CAGGCATATT TCTGAGAACC ATGAAAAAGA AGGAGAAAAT 
ATAAAGTCCG TGAATTCTGG TACTTGGATA GCATCCAACT CAGAACTTAC TCCCAGTGAA 
AGCCTTGCTA CTACTGATGA TGAAACTTTT GAGAAGAACT TTGAGAGAGA AACACATAAA 
ATAAGTGAGC AAAATGATGC TGATAATGCT AGTGTATCTT CAACTTGTGA GCCTTTTGCA 
ACAGATGTTC TGGGCGACAC TGTGATTCAC TTAGATCAGG CATTAGCCAG AATGAGAGAC 
TATACGCATA TGAAGATGGA GGCTGAAAGT AGTCCAGACG TCAGGTGCAC CTGCAGGATC 
ATTGAGGATG ACGATGGTGC TGCTGCCACA ACCACGATTA GTAATAATTT AGAAGAAACT 
CCTGTTATTG AAAATCATAG TTCACAGCAA CCCGTGAGTG AAGTTGCTAC CGTCCCGTGT 
CCTAGAATTG ATACTCAGCA ACTGGACCGG CAAATTAAAG CAATTATGAA AGAAGTCATT 
CCTTTTTTGA AGGAGCACAT GGATGAAGTA TGTTCTTCTC AACTTCTAAC CTCAGTAAGA 
CGCATGGTTT TGGTCCTTAC TCAGCAAAAC AATGAGAGCA AAGAGTTTGT AAAGTTCTTT 
CATAAGCAGC TTGGAAGTAT ATTACAGGAT TCACTCGCAA AATTTGCTGG CAGAAAACTG 
AAGGATTGTG GAGAAGATCT TCTTGTAGAG ATATCTGAAG TGTTATTTAA TGAATTGGCT 
TTCTTTAAGC TCATGCAAGA TTTGGATAAT AATAGTGTAA CTGTTAAACA GAGATGCAAA 
AGGAAAATAG AAGCTGCTGG AGTGATACAG TCTTATGCAA AAGAGGCCAA AAGGATTCTT 
GAAGGAGATC ACAGTTCACC TGCTGGAGAA ATTGATGATG AAGACAAAGA CAAGGATGAG 
ACGGAAACAG TTAAGCAGAC TCAAACGTCT GAGGTTTATG ATGGTGACGG CCCCAAAAAT 
GTGAGATCTG ATGTTTCTGA TCAAGAGGAT GATGAAGAAA GTGAAGAATG TCCAGTGTCT 
ATTAATTTGT CCAAAGCTGA AACTCAGGCT TTGACTAATT ACGGAAGTGG AGAAGATGAG 
AATGAAGATG AAGAAATAGA AGAATTTGAA GAGGGACCTG TAGATGTCCA GACGTCACTT 
CAGGCTAACA CTGAAACTAC AGAAGAAAAT GAACACGATG ATCAGGTTCC ACAGCATGAT 
TTTGAAAAAT CATCAGAAAG CAAAAATGTC CCATCAGAAC AAGAACCCAC TACTAGTAAA 
GGTGACCAGA ATAGCACTCC TGTGAAGCCC TGTTACCTCA GTATCGTGGA AAATGAGCAA 
CCTTTAAATA GCCCTGTCCA GAAGGACACT CTCACTACCA TCGACTCATC CAGTCAGCCT 
AACCCTTTGC CTTTACCTTT AACTGAAATT GAAACCTTGG TGCCTAGAGT CAAAGAAGTG 
AAATCTGCTC AGGAGACTCC TGAAAGTTCT TTGGCTGGAA GTCCTGATAC TGAATCTCCA 
GTGTTAGTGA ATGACTATGA AGCAGAATCT GGTAACATAA GTCAAAAATC TGATGAAGAA 
GACTTTGTGA AAGTTGAAGA TTTACCACTT AAACTGACAA TATATTCAGA GGCAGATCTA 
AGGAAGAAAA TGGCCGAAGA AGAACAGAAA AACCACTTAT CTGGTGAAAT ATGTGCAATG 
CAGACTGAAG AATTAGCTGG GAATTCTCAG ACACTAAAAG AACCTGAGCA AGTATGTGCT 
CAGTCTGCCA TCTTAAGATG GAAACCCTTA CCTGTGCCTT TTAAACTGTT GGTGGTGTCA 
TCAGGGAATT ATTGTAGCAA AGTAAGTTTC TTTTCTAATA ACTTTATATT AAAATTGAAA 
GTTTTAAATG GAAGAGAACA ATATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006080203.1
CDS	113..6499
Translation	MATGGGPFEEGVNDQDLPNWSNESVDDRLNNMDWGSQQKKANRSSEKNKRKFGVESDKRVTNDISPESSPGVGRRRTKTSLTFPHSRYMTQMSVPEQAELEKLKQRINFSDLDQINTNKSKDATVSPQKREMIGSAQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIREYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLQKILARENEEEDVRTIDSAVGSGSVAESTSLNIDVRSEASDATARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSEKVAETTPGHHTVPGRQPAHSPLHQRVPIRETTGSLSGVSITSELNEELNDLIQHFHNQLRDSQPPAVPDNRRQAESLSLTREISQSRNPSVSEHLPDEKVQLFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSFVPSSASPQRSVDQRSTTTAPSAPVVLTPVVNGESNNLTPSVPYPAASLVSQNQNENEGHLNPTEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENTKEEETEESEYDSEHENSEPVTNIRNPQVAATWNEVNSNSNAQCVSNNRDGRAVNSNCEINNRSAANIRALNMPPLSADCRYNREGEQEMPVAQGHEEEEEEEEEAEDEALSGASLSSHSSSLIIETPEDAEFEHKIQKLMAAKLKLRHLQNLVAMVQDDDAANQGVTSVEDFFPAEDTKQNANNTRGNANKTQKDAGVNEKAREKFYEAKLQQQQRELKQLQEERKKLIEIQEKIQELQKACPNLQLSAASVGNCPTKTHMPAVTSSPTVNENEASTNKSGFEPHDSSVVDNEVWSDMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETASPVAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGIIRTDEEEEEEEQDASSNDNFHMYPPNSANHNSYNVKETKNRWKNNRPFSADGNYRPLAKTRQQNISMQRQENLRWVSELSYVEEKEQWQEQINQLKKQLDFSVNICQTLMQDQQTLSCLLQTLLTSPYSVMPSSVASPQVHLIMHQLNQCYTQLTWQQNNVQRLKQMLSELMRQQNQHPEKPASKEGGSGTSHPPSPSLFCPFNFPTQPVNLFNLPGFTNFSSFAPSMNFSPLFPSNFGDFSQNISTPTEQQQPLAQNPSGKTEYMAFPKPFESSSSIGAEKQRNQKQPEEEVENSRTPWFYDQEGEVEKPFLKTGFAVSVEKTTNSNRKNQLDTSRRRRQFDEVSLESFNSMLDPVDPTTVTKTFKSRKASAQASLASKDKTPKSKSKKRHSTQLKSRVKNIGYESASVSSTCEPCKSRNRHSAQTEEPVQAKVLSRKNHEQLEKVIRCSRSTEISSAHARRILQQSNRNACNEAPETGSDFSMFEALRDTIYSEVATLISQNESRPHFLIELFHELQLLNTDYLRQRALYALQDIVSRHISENHEKEGENIKSVNSGTWIASNSELTPSESLATTDDETFEKNFERETHKISEQNDADNASVSSTCEPFATDVLGDTVIHLDQALARMRDYTHMKMEAESSPDVRCTCRIIEDDDGAAATTTISNNLEETPVIENHSSQQPVSEVATVPCPRIDTQQLDRQIKAIMKEVIPFLKEHMDEVCSSQLLTSVRRMVLVLTQQNNESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSVTVKQRCKRKIEAAGVIQSYAKEAKRILEGDHSSPAGEIDDEDKDKDETETVKQTQTSEVYDGDGPKNVRSDVSDQEDDEESEECPVSINLSKAETQALTNYGSGEDENEDEEIEEFEEGPVDVQTSLQANTETTEENEHDDQVPQHDFEKSSESKNVPSEQEPTTSKGDQNSTPVKPCYLSIVENEQPLNSPVQKDTLTTIDSSSQPNPLPLPLTEIETLVPRVKEVKSAQETPESSLAGSPDTESPVLVNDYEAESGNISQKSDEEDFVKVEDLPLKLTIYSEADLRKKMAEEEQKNHLSGEICAMQTEELAGNSQTLKEPEQVCAQSAILRWKPLPVPFKLLVVSSGNYCSKVSFFSNNFILKLKVLNGREQY

Target ORF information:

RefSeq Version	XM_006080141.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis pericentriolar material 1 (PCM1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006080141.1

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
AGCAATGAGA GTGTTGATGA CCGACTCAAC AATATGGATT GGGGCAGCCA GCAGAAGAAG 
GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA 
ACTAACGATA TTTCTCCGGA GTCATCACCG GGAGTTGGAA GACGAAGAAC AAAGACTTCA 
CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TCCCCGAGCA GGCAGAACTA 
GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGATTAATAC TAACAAGAGC 
AAAGATGCCA CTGTAAGTCC TCAAAAGAGA GAAATGATTG GATCAGCACA GTGTAAAGAG 
TTGTTTGCTT CTGCTTTAAG TAATGACCTT TTGCAAAACT GTCAAGTCTC TGAAGAAGAT 
GGAAGAGGAG AACCTGCAAT GGAGAGCAGC CAGATTGTAA GCAGGCTTGT TCAGATTCGT 
GAGTATATTA CTAAAGCTAG TTCCATGCGG GAAGACCTTG TAGAGAAAAA TGAAAGATCT 
GCTAATGTTG AGCGCCTCAC TCATCTAATA GATCACCTTA AAGAACAAGA AAAGTCATAT 
ATGAAATTTC TCCAGAAGAT TCTTGCTAGA GAAAATGAGG AGGAGGATGT TCGGACTATA 
GATTCAGCTG TGGGATCTGG TTCTGTAGCT GAGAGCACAT CGCTAAACAT AGATGTGCGG 
TCTGAGGCTT CAGATGCCAC GGCCAGAGAT CCTCAGCAGG AGCCTATGGA AGAGATAGAA 
AATTTGAAGA AACAACATGA TTTATTAAAA AGGATGTTAC AGCAGCAGGA GCAGCTGAGA 
GCTCTACAAG GACGACAAGC TGCTCTGCTT GCCCTGCAGC ATAAAGCTGA GCAGGCTATA 
GCTGTGATGG ATGATTCTGA AAAGGTTGCG GAGACAACTC CTGGCCATCA TACTGTACCA 
GGCAGACAGC CGGCTCATTC ACCTTTGCAT CAGAGAGTAC CCATAAGAGA AACTACAGGT 
AGCCTATCTG GAGTCAGTAT CACCTCTGAA CTAAACGAAG AATTGAATGA TTTAATTCAG 
CATTTTCATA ATCAGCTTCG TGATTCTCAG CCTCCAGCTG TTCCAGACAA TAGAAGACAA 
GCAGAAAGTC TTTCATTAAC TAGGGAGATT TCTCAGAGCA GAAATCCTTC AGTTTCAGAG 
CATTTACCTG ATGAGAAAGT CCAACTTTTT AGCAAGATGA GAGTGTTACA AGAAAAGAAA 
CAAAAAATGG ACAAACTTCT TGGAGAACTT CATACACTTC GAGATCAGCA TTTGAACAAT 
TCATCATTTG TGCCTTCTTC AGCCTCTCCA CAAAGGAGTG TGGATCAGAG AAGTACAACC 
ACAGCTCCTT CTGCTCCTGT AGTCTTAACA CCAGTGGTCA ATGGAGAATC CAATAACCTC 
ACGCCATCTG TTCCTTATCC TGCTGCTTCT CTAGTTTCTC AGAATCAGAA TGAAAATGAA 
GGCCACCTAA ATCCAACTGA AAAACTCCAG AAGTTAAATG AAGTTCGAAA GAGGTTGAAT 
GAACTAAGAG AATTAGTTCA TTATTATGAG CAAACATCAG ATATGATGAC AGATGCTGTG 
AATGAAAACA CAAAAGAAGA AGAAACTGAA GAATCAGAAT ATGATTCGGA GCATGAAAAC 
TCGGAACCTG TTACTAACAT TCGAAATCCA CAAGTGGCTG CCACCTGGAA CGAAGTAAAT 
AGTAATTCTA ATGCACAGTG TGTTTCCAAC AATAGAGATG GGCGAGCAGT TAATTCTAAC 
TGTGAAATTA ACAACAGGTC TGCTGCCAAC ATAAGGGCTC TAAACATGCC TCCTCTTTCA 
GCAGATTGTC GATACAATAG AGAAGGAGAA CAGGAGATGC CTGTTGCACA AGGTCATGAG 
GAGGAGGAGG AGGAGGAGGA GGAAGCAGAA GATGAGGCCC TCAGTGGAGC TTCACTGTCT 
AGTCACAGCA GCAGCCTGAT CATTGAGACT CCTGAAGATG CTGAATTTGA ACATAAGATC 
CAGAAGCTTA TGGCTGCAAA ACTGAAACTT AGACACTTAC AGAACCTTGT AGCCATGGTG 
CAGGACGATG ATGCAGCCAA TCAAGGAGTT ACTTCTGTGG AGGATTTCTT CCCAGCAGAA 
GACACCAAAC AAAATGCAAA TAACACTAGA GGAAATGCCA ATAAAACACA GAAAGATGCT 
GGAGTAAATG AAAAGGCAAG GGAGAAATTT TATGAGGCTA AACTACAGCA GCAACAGAGA 
GAACTCAAAC AATTGCAGGA AGAAAGAAAG AAACTGATTG AGATTCAAGA GAAAATTCAA 
GAATTGCAAA AGGCATGTCC TAACCTACAG CTGTCAGCTG CTAGTGTGGG TAATTGTCCC 
ACAAAAACAC ATATGCCAGC TGTTACTTCA TCCCCAACCG TTAATGAAAA TGAAGCCAGT 
ACAAACAAAT CTGGTTTTGA GCCTCATGAT TCTTCAGTGG TAGATAATGA GGTATGGTCA 
GATATGCGAA GACATGAAAT GTTAAGGGAG GAGCTACGAC AGAGAAGAAA GCAGCTGGAA 
GCTCTGATGG CTGAACATCA GAGGAGGCAA GGTCTGGCTG AAACTGCATC TCCAGTGGCT 
GTTTCACTGA GAAGTGATGG ATCTGAGAAC CTGTGTACAC CTCAGCAAAG CAGAACAGAA 
AAAACAATGG CAACTTGGGG AGGTTCTACC CAGTGTGCCC TAGATGAAGA AGGAGATGAA 
GATGGTTACC TTTCTGAAGG AATTATTCGG ACAGACGAAG AGGAGGAAGA GGAGGAGCAA 
GATGCCAGTT CCAATGATAA CTTTCATATG TATCCTCCAA ACAGTGCAAA TCATAATTCT 
TACAATGTAA AGGAAACTAA AAATAGGTGG AAGAACAATC GCCCTTTTTC AGCAGATGGA 
AATTATCGTC CTTTAGCCAA GACAAGACAG CAGAATATCA GCATGCAGCG TCAGGAAAAC 
CTTCGCTGGG TGTCCGAACT CTCTTATGTA GAAGAGAAAG AACAATGGCA AGAACAAATT 
AATCAGCTAA AGAAACAACT TGATTTCAGT GTCAATATTT GTCAGACTTT AATGCAAGAC 
CAGCAGACTC TGTCTTGTCT GCTACAAACT CTTCTCACGA GTCCTTATAG TGTTATGCCC 
AGCAGTGTTG CATCCCCGCA AGTGCACCTT ATAATGCACC AGTTGAATCA GTGCTATACC 
CAGCTAACGT GGCAGCAGAA CAATGTCCAG AGGTTGAAAC AAATGCTCAG TGAACTCATG 
CGCCAACAAA ATCAGCATCC AGAAAAACCT GCAAGCAAGG AAGGAGGCAG TGGTACATCA 
CACCCTCCTT CTCCCAGTTT ATTTTGTCCT TTCAACTTTC CAACTCAGCC TGTAAATCTG 
TTTAACCTAC CGGGGTTTAC TAATTTTTCG TCATTTGCAC CAAGTATGAA TTTCAGCCCT 
TTATTTCCTT CTAATTTTGG AGATTTTTCT CAAAATATTT CTACACCCAC TGAACAGCAG 
CAACCGTTAG CCCAGAATCC TTCAGGGAAA ACGGAATACA TGGCTTTTCC AAAACCTTTT 
GAAAGTAGTT CTTCTATTGG AGCGGAAAAA CAAAGGAATC AAAAACAGCC TGAAGAGGAG 
GTGGAGAACA GTAGGACACC ATGGTTTTAT GACCAAGAAG GTGAAGTAGA AAAGCCATTT 
CTCAAGACTG GATTTGCAGT GTCTGTAGAG AAAACAACAA ATAGTAATCG CAAAAATCAA 
CTAGATACCA GTAGGAGAAG ACGCCAGTTT GATGAAGTGT CCTTAGAAAG CTTTAACAGT 
ATGCTTGACC CAGTAGATCC AACAACAGTA ACTAAAACAT TCAAGTCAAG AAAAGCATCT 
GCACAGGCCA GCCTGGCATC TAAAGATAAA ACTCCCAAAT CAAAGAGTAA GAAGAGGCAT 
TCTACTCAGC TGAAAAGCAG AGTGAAAAAT ATCGGGTATG AAAGTGCCAG TGTGTCTAGC 
ACATGTGAAC CTTGCAAAAG TAGAAACAGA CATTCAGCCC AAACTGAAGA GCCTGTTCAA 
GCAAAAGTAC TCAGCAGAAA GAATCATGAG CAGCTGGAAA AAGTCATAAG ATGTAGTAGG 
TCTACAGAAA TATCTTCAGC ACATGCTAGG AGAATACTAC AGCAGTCTAA CAGAAATGCA 
TGCAATGAAG CGCCAGAAAC CGGGAGTGAT TTTTCCATGT TCGAAGCTTT GCGGGATACC 
ATTTATTCTG AAGTAGCTAC GTTGATTTCT CAAAATGAAT CTCGTCCACA TTTTCTTATT 
GAACTCTTCC ATGAGCTTCA GCTACTTAAT ACAGACTATT TGAGACAGAG GGCCTTATAT 
GCATTGCAGG ACATAGTATC CAGGCATATT TCTGAGAACC ATGAAAAAGA AGGAGAAAAT 
ATAAAGTCCG TGAATTCTGG TACTTGGATA GCATCCAACT CAGAACTTAC TCCCAGTGAA 
AGCCTTGCTA CTACTGATGA TGAAACTTTT GAGAAGAACT TTGAGAGAGA AACACATAAA 
ATAAGTGAGC AAAATGATGC TGATAATGCT AGTGTATCTT CAACTTGTGA GCCTTTTGCA 
ACAGATGTTC TGGGCGACAC TGTGATTCAC TTAGATCAGG CATTAGCCAG AATGAGAGAC 
TATACGCATA TGAAGATGGA GGCTGAAAGT AGTCCAGACG TCAGGTGCAC CTGCAGGATC 
ATTGAGGATG ACGATGGTGC TGCTGCCACA ACCACGATTA GTAATAATTT AGAAGAAACT 
CCTGTTATTG AAAATCATAG TTCACAGCAA CCCGTGAGTG AAGTTGCTAC CGTCCCGTGT 
CCTAGAATTG ATACTCAGCA ACTGGACCGG CAAATTAAAG CAATTATGAA AGAAGTCATT 
CCTTTTTTGA AGGAGCACAT GGATGAAGTA TGTTCTTCTC AACTTCTAAC CTCAGTAAGA 
CGCATGGTTT TGGTCCTTAC TCAGCAAAAC AATGAGAGCA AAGAGTTTGT AAAGTTCTTT 
CATAAGCAGC TTGGAAGTAT ATTACAGGAT TCACTCGCAA AATTTGCTGG CAGAAAACTG 
AAGGATTGTG GAGAAGATCT TCTTGTAGAG ATATCTGAAG TGTTATTTAA TGAATTGGCT 
TTCTTTAAGC TCATGCAAGA TTTGGATAAT AATAGTGTAA CTGTTAAACA GAGATGCAAA 
AGGAAAATAG AAGCTGCTGG AGTGATACAG TCTTATGCAA AAGAGGCCAA AAGGATTCTT 
GAAGGAGATC ACAGTTCACC TGCTGGAGAA ATTGATGATG AAGACAAAGA CAAGGATGAG 
ACGGAAACAG TTAAGCAGAC TCAAACGTCT GAGGTTTATG ATGGTGACGG CCCCAAAAAT 
GTGAGATCTG ATGTTTCTGA TCAAGAGGAT GATGAAGAAA GTGAAGAATG TCCAGTGTCT 
ATTAATTTGT CCAAAGCTGA AACTCAGGCT TTGACTAATT ACGGAAGTGG AGAAGATGAG 
AATGAAGATG AAGAAATAGA AGAATTTGAA GAGGGACCTG TAGATGTCCA GACGTCACTT 
CAGGCTAACA CTGAAACTAC AGAAGAAAAT GAACACGATG ATCAGGTTCC ACAGCATGAT 
TTTGAAAAAT CATCAGAAAG CAAAAATGTC CCATCAGAAC AAGAACCCAC TACTAGTAAA 
GGTGACCAGA ATAGCACTCC TGTGAAGCCC TGTTACCTCA GTATCGTGGA AAATGAGCAA 
CCTTTAAATA GCCCTGTCCA GAAGGACACT CTCACTACCA TCGACTCATC CAGTCAGCCT 
AACCCTTTGC CTTTACCTTT AACTGAAATT GAAACCTTGG TGCCTAGAGT CAAAGAAGTG 
AAATCTGCTC AGGAGACTCC TGAAAGTTCT TTGGCTGGAA GTCCTGATAC TGAATCTCCA 
GTGTTAGTGA ATGACTATGA AGCAGAATCT GGTAACATAA GTCAAAAATC TGATGAAGAA 
GACTTTGTGA AAGTTGAAGA TTTACCACTT AAACTGACAA TATATTCAGA GGCAGATCTA 
AGGAAGAAAA TGGCCGAAGA AGAACAGAAA AACCACTTAT CTGGTGAAAT ATGTGCAATG 
CAGACTGAAG AATTAGCTGG GAATTCTCAG ACACTAAAAG AACCTGAGCA AGTATGTGCT 
CAGTCTGCCA TCTTAAGATG GAAACCCTTA CCTGTGCCTT TTAAACTGTT GGTGGTGTCA 
TCAGGGAATT ATTGTAGCAA AGTAAGTTTC TTTTCTAATA ACTTTATATT AAAATTGAAA 
GTTTTAAATG GAAGAGAACA ATATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	PCM1
Entrez Gene ID	102412163
Full Name	pericentriolar material 1
Gene Type	protein-coding
Organism	Bubalus bubalis(water buffalo)

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

PCM1 cDNA ORF clone, Bubalus bubalis(water buffalo)

Gene

Clones

gRNAs

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