PTCH2 cDNA ORF clone, Bubalus bubalis(water buffalo)

The following PTCH2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PTCH2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBu68868 XM_025288854.1
Latest version!
Bubalus bubalis patched 2 (PTCH2), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OBu10278 XM_006052576.1
Latest version!
Bubalus bubalis patched 2 (PTCH2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OBu10279 XM_006052577.1
Latest version!
Bubalus bubalis patched 2 (PTCH2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OBu10280 XM_006052578.1
Latest version!
Bubalus bubalis patched 2 (PTCH2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OBu10281 XM_006052579.1
Latest version!
Bubalus bubalis patched 2 (PTCH2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OBu10282 XM_006052580.1
Latest version!
Bubalus bubalis patched 2 (PTCH2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OBu68862 XM_006052576.2
Latest version!
Bubalus bubalis patched 2 (PTCH2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBu68863 XM_006052577.2
Latest version!
Bubalus bubalis patched 2 (PTCH2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBu68864 XM_006052578.2
Latest version!
Bubalus bubalis patched 2 (PTCH2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBu68865 XM_025288853.1
Latest version!
Bubalus bubalis patched 2 (PTCH2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBu68866 XM_006052579.2
Latest version!
Bubalus bubalis patched 2 (PTCH2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBu68867 XM_006052580.2
Latest version!
Bubalus bubalis patched 2 (PTCH2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBu68868
    Clone ID Related Accession (Same CDS sequence) XM_025288854.1
    Accession Version XM_025288854.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2436bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product protein patched homolog 2 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037550.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGGCTCGGC CGCCACCTCT CGGGGAGCTG CCCCCGGGCT ACACACCCCC AGCTCGACCT 
    GCAACACCCC AGATCCTAGC TGGGAGCCTG AAGGCTCCGC TCTGGCTCCG TGCTTACTTC
    CAGGGCCTGC TCTTCTCTTT GGGCTGCGGG ATCCAGAGAC ACTGTGGCAA AGTGCTCTTC
    CTGGGACTGT TGGCCTTTGG GGCCCTAGCA CTGGGTCTCC GCGTGGCCAT CATTGAGACA
    GACCTAGAAC AGCTCTGGGT GGAAGTGGGC AGCCGGGTGA GCCAGGAACT GGAATACACC
    AAGGAGAAGC TGGGGGAGGA GGCTGCGTAT ACCTCCCAGA TGTTGATACA GACCCCGCGT
    CAGGAGGGGG AGAACGTCCT CACGCCCGAG GCACTTGACC TCCACCTCCA GGCAGCCCTC
    ACCGCCAGTA AAGTGCAAGT ATCCCTCTAT GGAAAGTCCT GGGATCTGAA CAAAATCTGC
    TACAAGTCAG GAGTTCCCCT AATTGAAAAT GGAATGATTG AGCGGATGAT TGAAAAGCTG
    TTTCCCTGCG TGATCCTCAC CCCCCTCGAC TGCTTCTGGG AGGGAGCCAA ACTCCAAGGG
    GGCTCTGCCT ACTTGCCGGG CCGCCCAGAC ATCCAGTGGA CCAACCTGGA CCCAGAGCAG
    CTGCTTGAGG AGCTGGGCCC CTTTGCCTCC CTTGAGGGCT TCCGGGAGCT GCTAGACAAG
    GCACAGGTGG GCCAGGCCTA CGTGGGTCGG CCCTGTCTGC ACCCTGACGA CCTCCACTGC
    CCACCTAGTG CCCCTAACCA TCACAGCAAG CAGGCTCCCA ACGTGGCTCA GGAGTTGAGT
    GGGGGCTGCC ATGGCTTCTC CCACAAGTTT ATGCACTGGC AGGAGGAGCT GCTGCTGGGA
    GGCATGGCCA GAGACCCCCA AGGACAGCTG CTGAGGGCAG AGGCCCTGCA GAGCACCTTC
    CTGCTAATGA GCCCCCGCCA GCTCTACGAG CACTTCCGAG GTGACTACCA GACGCATGAC
    ATCGGCTGGA GCGAGGAGCA GGCCGGCACG GTCCTTCAGG CCTGGCAGCG ACGCTTTGTG
    CAGCTGGCCC AGGAGGCCCT GCCCGAGAAC GCATCCCAGC AGATTCACGC CTTCTCCTCT
    ACCACCCTGG ATGACATCCT GCACGCCTTC TCTGAAGTCA GCGCTGCTCG CGTGGCGGGG
    GGCTACCTGC TCATGCTAGC CTATGCCTGT GTGACGATGC TGCGGTGGGA CTGTGCTCAG
    TCCCAGGGTG CAGTGGGCCT CGCTGGTGTG CTGCTGGTGG CCCTGGCAGT GGCCTCGGGC
    CTGGGGCTCT GTGCCCTGTT GGGCATCGCC TTCAACGCTG CCACTACCCA GGTGCTGCCT
    TTCTTGGCAC TGGGCATCGG CGTGGATGAC ATATTCCTGC TGGCACACGC CTTCACAGAG
    GCTCCACCTG GCACCCCTCT CCAGGAGCGC ACGGGGGAGT GTCTGCGGCG CACAGGCACC
    GGTGTCACAC TCACATCCGT CAACAATATG GTCGCCTTCT TCATGGCCGC CCTAGTCCCC
    ATCCCTGCAC TTCGGGCCTT CTCCTTGCAG GCAGCCATAG TGGTGGGCTG CAACTTTGCA
    GCCGTGATGC TTGTCTTCCC AGCGGTCCTC AGCCTGGACC TGCAGCGGCG CCACTGCCGG
    CGCCTGGATG TGCTCTGCTG CTTCTCTAGC CCCTGCTCTG CTCAGGTGAT TCAGATTCTG
    CCCCAGGAGC TGGGGAACGG GACGGTACCA GTGGGCGTTG CCCACCTGAC TGCCACGGTT
    CAAGCCTTTG CCCACTGTGA AGCCAGGAGC CAACATGTGG TCACCATCTT GCCTCCCCAA
    GCTCACCTGG TGCCGCCACC TTCTGACCCT TTGGGCTCTG AGCTCTTCAG CCCAGGAGGG
    TCCACACGGG ACCTTCTAGG GCAGGAGGAG GGGACAGGGC AGAAGGCAGC CTGCAAGTCC
    CTGCCCTGTG CCCGCTGGAA TCTTGCCCAT TTCGCCCGCT CTCAGTTTGC ACCCTTGTTG
    CTCCAGTCCC CCACCAAGCT TAGTCACCTT TCCCCACAGG CTGTAGTACT GGTACTTTTT
    GGGGCTCTCC TGGGCCTGAG CCTCTATGGA GCGACCTTGG TGCAGGACGG TCTGGCCCTG
    ACTGATGTGG TGCCGCGTGG CACCAAGGAG CATGCCTTCC TGAGCGCCCA GCTCAGCGCT
    TCAGTTCCCT CAAGGCCGTG CTGCCCCCAC CGGCCACCCA GGCACCCCGC ACCTGGCTGC
    ACTACTACCG CAGCTGGCTA CAGGGAATCC AGGCTGCGTT TGACCAGGAC TGGGCTTCTG
    GGCGCATCAC CCGCCACTCA TACCGCAATG GCTCTGAGGA TGGGGCCCTG GCATACAAGC
    TGCTTGTCCA GACCGGGGAT GCCCAGGAGC CTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025144639.1
    CDS438..2873
    Translation

    Target ORF information:

    RefSeq Version XM_025288854.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis patched 2 (PTCH2), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025288854.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGGCTCGGC CGCCACCTCT CGGGGAGCTG CCCCCGGGCT ACACACCCCC AGCTCGACCT 
    GCAACACCCC AGATCCTAGC TGGGAGCCTG AAGGCTCCGC TCTGGCTCCG TGCTTACTTC
    CAGGGCCTGC TCTTCTCTTT GGGCTGCGGG ATCCAGAGAC ACTGTGGCAA AGTGCTCTTC
    CTGGGACTGT TGGCCTTTGG GGCCCTAGCA CTGGGTCTCC GCGTGGCCAT CATTGAGACA
    GACCTAGAAC AGCTCTGGGT GGAAGTGGGC AGCCGGGTGA GCCAGGAACT GGAATACACC
    AAGGAGAAGC TGGGGGAGGA GGCTGCGTAT ACCTCCCAGA TGTTGATACA GACCCCGCGT
    CAGGAGGGGG AGAACGTCCT CACGCCCGAG GCACTTGACC TCCACCTCCA GGCAGCCCTC
    ACCGCCAGTA AAGTGCAAGT ATCCCTCTAT GGAAAGTCCT GGGATCTGAA CAAAATCTGC
    TACAAGTCAG GAGTTCCCCT AATTGAAAAT GGAATGATTG AGCGGATGAT TGAAAAGCTG
    TTTCCCTGCG TGATCCTCAC CCCCCTCGAC TGCTTCTGGG AGGGAGCCAA ACTCCAAGGG
    GGCTCTGCCT ACTTGCCGGG CCGCCCAGAC ATCCAGTGGA CCAACCTGGA CCCAGAGCAG
    CTGCTTGAGG AGCTGGGCCC CTTTGCCTCC CTTGAGGGCT TCCGGGAGCT GCTAGACAAG
    GCACAGGTGG GCCAGGCCTA CGTGGGTCGG CCCTGTCTGC ACCCTGACGA CCTCCACTGC
    CCACCTAGTG CCCCTAACCA TCACAGCAAG CAGGCTCCCA ACGTGGCTCA GGAGTTGAGT
    GGGGGCTGCC ATGGCTTCTC CCACAAGTTT ATGCACTGGC AGGAGGAGCT GCTGCTGGGA
    GGCATGGCCA GAGACCCCCA AGGACAGCTG CTGAGGGCAG AGGCCCTGCA GAGCACCTTC
    CTGCTAATGA GCCCCCGCCA GCTCTACGAG CACTTCCGAG GTGACTACCA GACGCATGAC
    ATCGGCTGGA GCGAGGAGCA GGCCGGCACG GTCCTTCAGG CCTGGCAGCG ACGCTTTGTG
    CAGCTGGCCC AGGAGGCCCT GCCCGAGAAC GCATCCCAGC AGATTCACGC CTTCTCCTCT
    ACCACCCTGG ATGACATCCT GCACGCCTTC TCTGAAGTCA GCGCTGCTCG CGTGGCGGGG
    GGCTACCTGC TCATGCTAGC CTATGCCTGT GTGACGATGC TGCGGTGGGA CTGTGCTCAG
    TCCCAGGGTG CAGTGGGCCT CGCTGGTGTG CTGCTGGTGG CCCTGGCAGT GGCCTCGGGC
    CTGGGGCTCT GTGCCCTGTT GGGCATCGCC TTCAACGCTG CCACTACCCA GGTGCTGCCT
    TTCTTGGCAC TGGGCATCGG CGTGGATGAC ATATTCCTGC TGGCACACGC CTTCACAGAG
    GCTCCACCTG GCACCCCTCT CCAGGAGCGC ACGGGGGAGT GTCTGCGGCG CACAGGCACC
    GGTGTCACAC TCACATCCGT CAACAATATG GTCGCCTTCT TCATGGCCGC CCTAGTCCCC
    ATCCCTGCAC TTCGGGCCTT CTCCTTGCAG GCAGCCATAG TGGTGGGCTG CAACTTTGCA
    GCCGTGATGC TTGTCTTCCC AGCGGTCCTC AGCCTGGACC TGCAGCGGCG CCACTGCCGG
    CGCCTGGATG TGCTCTGCTG CTTCTCTAGC CCCTGCTCTG CTCAGGTGAT TCAGATTCTG
    CCCCAGGAGC TGGGGAACGG GACGGTACCA GTGGGCGTTG CCCACCTGAC TGCCACGGTT
    CAAGCCTTTG CCCACTGTGA AGCCAGGAGC CAACATGTGG TCACCATCTT GCCTCCCCAA
    GCTCACCTGG TGCCGCCACC TTCTGACCCT TTGGGCTCTG AGCTCTTCAG CCCAGGAGGG
    TCCACACGGG ACCTTCTAGG GCAGGAGGAG GGGACAGGGC AGAAGGCAGC CTGCAAGTCC
    CTGCCCTGTG CCCGCTGGAA TCTTGCCCAT TTCGCCCGCT CTCAGTTTGC ACCCTTGTTG
    CTCCAGTCCC CCACCAAGCT TAGTCACCTT TCCCCACAGG CTGTAGTACT GGTACTTTTT
    GGGGCTCTCC TGGGCCTGAG CCTCTATGGA GCGACCTTGG TGCAGGACGG TCTGGCCCTG
    ACTGATGTGG TGCCGCGTGG CACCAAGGAG CATGCCTTCC TGAGCGCCCA GCTCAGCGCT
    TCAGTTCCCT CAAGGCCGTG CTGCCCCCAC CGGCCACCCA GGCACCCCGC ACCTGGCTGC
    ACTACTACCG CAGCTGGCTA CAGGGAATCC AGGCTGCGTT TGACCAGGAC TGGGCTTCTG
    GGCGCATCAC CCGCCACTCA TACCGCAATG GCTCTGAGGA TGGGGCCCTG GCATACAAGC
    TGCTTGTCCA GACCGGGGAT GCCCAGGAGC CTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu10278
    Clone ID Related Accession (Same CDS sequence) XM_006052576.1
    Accession Version XM_006052576.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3771bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2014-03-11
    Organism Bubalus bubalis(water buffalo)
    Product protein patched homolog 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005784468.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCTCGGC CGCCACCTCT CGGGGAGCTG CCCCCGGGCT ACACACCCCC AGCTCGACCT 
    GCAACACCCC AGATCCTAGC TGGGAGCCTG AAGGCTCCGC TCTGGCTCCG TGCTTACTTC
    CAGGGCCTGC TCTTCTCTTT GGGCTGCGGG ATCCAGAGAC ACTGTGGCAA AGTGCTCTTC
    CTGGGACTGT TGGCCTTTGG GGCCCTAGCA CTGGGTCTCC GCGTGGCCAT CATTGAGACA
    GACCTAGAAC AGCTCTGGGT GGAAGTGGGC AGCCGGGTGA GCCAGGAACT GGAATACACC
    AAGGAGAAGC TGGGGGAGGA GGCTGCGTAT ACCTCCCAGA TGTTGATACA GACCCCGCGT
    CAGGAGGGGG AGAACGTCCT CACGCCCGAG GCACTTGACC TCCACCTCCA GGCAGCCCTC
    ACCGCCAGTA AAGTGCAAGT ATCCCTCTAT GGAAAGTCCT GGGATCTGAA CAAAATCTGC
    TACAAGTCAG GAGTTCCCCT AATTGAAAAT GGAATGATTG AGCGGATGAT TGAAAAGCTG
    TTTCCCTGCG TGATCCTCAC CCCCCTCGAC TGCTTCTGGG AGGGAGCCAA ACTCCAAGGG
    GGCTCTGCCT ACTTGCCGGG CCGCCCAGAC ATCCAGTGGA CCAACCTGGA CCCAGAGCAG
    CTGCTTGAGG AGCTGGGCCC CTTTGCCTCC CTTGAGGGCT TCCGGGAGCT GCTAGACAAG
    GCACAGGTGG GCCAGGCCTA CGTGGGTCGG CCCTGTCTGC ACCCTGACGA CCTCCACTGC
    CCACCTAGTG CCCCTAACCA TCACAGCAAG CAGGCTCCCA ACGTGGCTCA GGAGTTGAGT
    GGGGGCTGCC ATGGCTTCTC CCACAAGTTT ATGCACTGGC AGGAGGAGCT GCTGCTGGGA
    GGCATGGCCA GAGACCCCCA AGGACAGCTG CTGAGGGCAG AGGCCCTGCA GAGCACCTTC
    CTGCTAATGA GCCCCCGCCA GCTCTACGAG CACTTCCGAG GTGACTACCA GACGCATGAC
    ATCGGCTGGA GCGAGGAGCA GGCCGGCACG GTCCTTCAGG CCTGGCAGCG ACGCTTTGTG
    CAGCTGGCCC AGGAGGCCCT GCCCGAGAAC GCATCCCAGC AGATTCACGC CTTCTCCTCT
    ACCACCCTGG ATGACATCCT GCACGCCTTC TCTGAAGTCA GCGCTGCTCG CGTGGCGGGG
    GGCTACCTGC TCATGCTAGC CTATGCCTGT GTGACGATGC TGCGGTGGGA CTGTGCTCAG
    TCCCAGGGTG CAGTGGGCCT CGCTGGTGTG CTGCTGGTGG CCCTGGCAGT GGCCTCGGGC
    CTGGGGCTCT GTGCCCTGTT GGGCATCGCC TTCAACGCTG CCACTACCCA GGTGCTGCCT
    TTCTTGGCAC TGGGCATCGG CGTGGATGAC ATATTCCTGC TGGCACACGC CTTCACAGAG
    GCTCCACCTG GCACCCCTCT CCAGGAGCGC ACGGGGGAGT GTCTGCGGCG CACAGGCACC
    GGTGTCACAC TCACATCCGT CAACAATATG GTCGCCTTCT TCATGGCCGC CCTAGTCCCC
    ATCCCTGCAC TTCGGGCCTT CTCCTTGCAG GCAGCCATAG TGGTGGGCTG CAACTTTGCA
    GCCGTGATGC TTGTCTTCCC AGCGGTCCTC AGCCTGGACC TGCAGCGGCG CCACTGCCGG
    CGCCTGGATG TGCTCTGCTG CTTCTCTAGC CCCTGCTCTG CTCGGGTGAT TCAGATTCTG
    CCCCAGGAGC TGGGGAACGG GACGGTACCA GTGGGCGTTG CCCACCTGAC TGCCACGGTT
    CAAGCCTTTG CCCACTGTGA AGCCAGGAGC CAACATGTGG TCACCATCTT GCCTCCCCAA
    GCTCACCTGG TGCCGCCACC TTCTGACCCT TTGGGCTCTG AGCTCTTCAG CCCAGGAGGG
    TCCACACGGG ACCTTCTAGG GCAGGAGGAG GGGACAGGGC AGAAGGCAGC CTGCAAGTCC
    CTGCCCTGTG CCCGCTGGAA TCTTGCCCAT TTCGCCCGCT CTCAGTTTGC ACCCTTGTTG
    CTCCAGTCCC CCACCAAGCT TAGTCACCTT TCCCCACAGG CTGTAGTACT GGTACTTTTT
    GGGGCTCTCC TGGGCCTGAG CCTCTATGGA GCGACCTTGG TGCAGGACGG TCTGGCCCTG
    ACTGATGTGG TGCCGCGTGG CACCAAGGAG CATGCCTTCC TGAGCGCCCA GCTCAGGTAC
    TTCTCTCTGT ACGAGGTGGC TCTGGTGACA CAGGGTGGCT TTGACTACGC CCACTCCCAA
    CGCGCCCTCT TTGATCTGCA CCAGCGCTTC AGTTCCCTCA AGGCCGTGCT GCCCCCACCG
    GCCACCCAGG CACCCCGCAC CTGGCTGCAC TACTACCGCA GCTGGCTACA GGGAATCCAG
    GCTGCATTTG ACCAGGACTG GGCTTCTGGG CGCATCACCC GCCACTCATA CCGCAATGGC
    TCTGAGGATG GGGCCCTGGC ATACAAGCTG CTTGTCCAGA CCGGGGATGC CCAGGAGCCT
    CTAGATTTCA GCCAGCTGAC CACCAAGAAG CTGGTGGACA AGGAAGGGCT GATCCCACCC
    GAGCTCTTCT ACATGGGGCT GACCGTGTGG GTGAGCAGTG ACCCCCTGGG TCTGGCAGCC
    TCACAGGCCA ACTTCTATCC CCCACCTCCC GAGTGGCTGC ATGACAAGTA CGATACCACG
    GGGGAGAATC TTCGCAGTGA GTTCTGGGGG AGCCCGAACT GCCTGGGCCC AAGCCCACCC
    AGGCCCTCCC CTGAAGCCCT GCCCACTGCT GCCAGAGCTC ACCGGCCGCC CCTCCCTCTC
    CCTCCGACCC CTCCCTCCAC AGTCCCGGCG GCCCAGCCCT TGGAGTTTGC CCAGTTCCCC
    TTCCTCCTGC GCGGCCTCCA GAAGACTGCA GACTTCGTGG AGGCCATCGA GGGGGCCCGG
    GCAGCGTGTG CCGAGGCCGG CCAGGCCGGG GTGCATGCCT ACCCCAGCGG CTCCCCCTTC
    CTCTTCTGGG AGCAGTACCT GAGCCTGCGG CGCTGCTTCC TGCTGGCGGT CTGCATCCTG
    CTGCTGTGTA CTTTCCTCGT CTGTGCCCTG CTGCTGCTCA ACCCCTGGAC GGCTGTGCTC
    ATAGTACTGG TCCTGGCGAT GATGACTGTG GAGCTCTTTG GCATCATGGG TTTCCTGGGC
    ATCAAGCTGA GTGCCATCCC GGTGGTGATT CTTGTGGCCT CCATAGGCAT CGGTGTCGAG
    TTCACTGTCC ACGTGGCTCT GGGCTTCCTG ACCGCTCAGG GTAGCCGGAA CCTGCGGGCT
    GCCCGGGCCC TGGAGCGCAC ATTTGCCCCA GTGACCGATG GGGCCATCTC CACGTTGCTA
    GGTCTGCTCA TGCTTGCTGG CTCCAACTTT GACTTCATCG TAAGGTACTT CTTTGTGGTG
    CTGACAATAC TTACACTCCT GGGCCTCCTC CACGGGCTCG TGCTGCTGCC GGTACTGCTG
    TCCATCCTGG GCCCCCCACC AGAGGTGGTA CAGATGTACA AGGAGAGCAC AGAGGTCCTG
    AGCCCCCCAG CTCCACAGGG AGGAGGGCTT AGGTGGGGGG TACCCTCCAC CCTGCCCCAG
    AGCTTTGCCA GAGTGACTAC CTCCATGACT GTGGCCCTCC ACCCACCCCC ACTGCCTGGT
    GCCTACATCC ACCCAGCCTC TGAGGAGCCT ACTTGGTCAC CTGCTGCCAC ACCAGCTGCC
    AATGGCCCCA GCAACCTCGG GTCTAGGGGA CTGTGTCCAG CCACGGGGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006052638.1
    CDS1801..5571
    Translation

    Target ORF information:

    RefSeq Version XM_006052576.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis patched 2 (PTCH2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006052576.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCTCGGC CGCCACCTCT CGGGGAGCTG CCCCCGGGCT ACACACCCCC AGCTCGACCT 
    GCAACACCCC AGATCCTAGC TGGGAGCCTG AAGGCTCCGC TCTGGCTCCG TGCTTACTTC
    CAGGGCCTGC TCTTCTCTTT GGGCTGCGGG ATCCAGAGAC ACTGTGGCAA AGTGCTCTTC
    CTGGGACTGT TGGCCTTTGG GGCCCTAGCA CTGGGTCTCC GCGTGGCCAT CATTGAGACA
    GACCTAGAAC AGCTCTGGGT GGAAGTGGGC AGCCGGGTGA GCCAGGAACT GGAATACACC
    AAGGAGAAGC TGGGGGAGGA GGCTGCGTAT ACCTCCCAGA TGTTGATACA GACCCCGCGT
    CAGGAGGGGG AGAACGTCCT CACGCCCGAG GCACTTGACC TCCACCTCCA GGCAGCCCTC
    ACCGCCAGTA AAGTGCAAGT ATCCCTCTAT GGAAAGTCCT GGGATCTGAA CAAAATCTGC
    TACAAGTCAG GAGTTCCCCT AATTGAAAAT GGAATGATTG AGCGGATGAT TGAAAAGCTG
    TTTCCCTGCG TGATCCTCAC CCCCCTCGAC TGCTTCTGGG AGGGAGCCAA ACTCCAAGGG
    GGCTCTGCCT ACTTGCCGGG CCGCCCAGAC ATCCAGTGGA CCAACCTGGA CCCAGAGCAG
    CTGCTTGAGG AGCTGGGCCC CTTTGCCTCC CTTGAGGGCT TCCGGGAGCT GCTAGACAAG
    GCACAGGTGG GCCAGGCCTA CGTGGGTCGG CCCTGTCTGC ACCCTGACGA CCTCCACTGC
    CCACCTAGTG CCCCTAACCA TCACAGCAAG CAGGCTCCCA ACGTGGCTCA GGAGTTGAGT
    GGGGGCTGCC ATGGCTTCTC CCACAAGTTT ATGCACTGGC AGGAGGAGCT GCTGCTGGGA
    GGCATGGCCA GAGACCCCCA AGGACAGCTG CTGAGGGCAG AGGCCCTGCA GAGCACCTTC
    CTGCTAATGA GCCCCCGCCA GCTCTACGAG CACTTCCGAG GTGACTACCA GACGCATGAC
    ATCGGCTGGA GCGAGGAGCA GGCCGGCACG GTCCTTCAGG CCTGGCAGCG ACGCTTTGTG
    CAGCTGGCCC AGGAGGCCCT GCCCGAGAAC GCATCCCAGC AGATTCACGC CTTCTCCTCT
    ACCACCCTGG ATGACATCCT GCACGCCTTC TCTGAAGTCA GCGCTGCTCG CGTGGCGGGG
    GGCTACCTGC TCATGCTAGC CTATGCCTGT GTGACGATGC TGCGGTGGGA CTGTGCTCAG
    TCCCAGGGTG CAGTGGGCCT CGCTGGTGTG CTGCTGGTGG CCCTGGCAGT GGCCTCGGGC
    CTGGGGCTCT GTGCCCTGTT GGGCATCGCC TTCAACGCTG CCACTACCCA GGTGCTGCCT
    TTCTTGGCAC TGGGCATCGG CGTGGATGAC ATATTCCTGC TGGCACACGC CTTCACAGAG
    GCTCCACCTG GCACCCCTCT CCAGGAGCGC ACGGGGGAGT GTCTGCGGCG CACAGGCACC
    GGTGTCACAC TCACATCCGT CAACAATATG GTCGCCTTCT TCATGGCCGC CCTAGTCCCC
    ATCCCTGCAC TTCGGGCCTT CTCCTTGCAG GCAGCCATAG TGGTGGGCTG CAACTTTGCA
    GCCGTGATGC TTGTCTTCCC AGCGGTCCTC AGCCTGGACC TGCAGCGGCG CCACTGCCGG
    CGCCTGGATG TGCTCTGCTG CTTCTCTAGC CCCTGCTCTG CTCGGGTGAT TCAGATTCTG
    CCCCAGGAGC TGGGGAACGG GACGGTACCA GTGGGCGTTG CCCACCTGAC TGCCACGGTT
    CAAGCCTTTG CCCACTGTGA AGCCAGGAGC CAACATGTGG TCACCATCTT GCCTCCCCAA
    GCTCACCTGG TGCCGCCACC TTCTGACCCT TTGGGCTCTG AGCTCTTCAG CCCAGGAGGG
    TCCACACGGG ACCTTCTAGG GCAGGAGGAG GGGACAGGGC AGAAGGCAGC CTGCAAGTCC
    CTGCCCTGTG CCCGCTGGAA TCTTGCCCAT TTCGCCCGCT CTCAGTTTGC ACCCTTGTTG
    CTCCAGTCCC CCACCAAGCT TAGTCACCTT TCCCCACAGG CTGTAGTACT GGTACTTTTT
    GGGGCTCTCC TGGGCCTGAG CCTCTATGGA GCGACCTTGG TGCAGGACGG TCTGGCCCTG
    ACTGATGTGG TGCCGCGTGG CACCAAGGAG CATGCCTTCC TGAGCGCCCA GCTCAGGTAC
    TTCTCTCTGT ACGAGGTGGC TCTGGTGACA CAGGGTGGCT TTGACTACGC CCACTCCCAA
    CGCGCCCTCT TTGATCTGCA CCAGCGCTTC AGTTCCCTCA AGGCCGTGCT GCCCCCACCG
    GCCACCCAGG CACCCCGCAC CTGGCTGCAC TACTACCGCA GCTGGCTACA GGGAATCCAG
    GCTGCATTTG ACCAGGACTG GGCTTCTGGG CGCATCACCC GCCACTCATA CCGCAATGGC
    TCTGAGGATG GGGCCCTGGC ATACAAGCTG CTTGTCCAGA CCGGGGATGC CCAGGAGCCT
    CTAGATTTCA GCCAGCTGAC CACCAAGAAG CTGGTGGACA AGGAAGGGCT GATCCCACCC
    GAGCTCTTCT ACATGGGGCT GACCGTGTGG GTGAGCAGTG ACCCCCTGGG TCTGGCAGCC
    TCACAGGCCA ACTTCTATCC CCCACCTCCC GAGTGGCTGC ATGACAAGTA CGATACCACG
    GGGGAGAATC TTCGCAGTGA GTTCTGGGGG AGCCCGAACT GCCTGGGCCC AAGCCCACCC
    AGGCCCTCCC CTGAAGCCCT GCCCACTGCT GCCAGAGCTC ACCGGCCGCC CCTCCCTCTC
    CCTCCGACCC CTCCCTCCAC AGTCCCGGCG GCCCAGCCCT TGGAGTTTGC CCAGTTCCCC
    TTCCTCCTGC GCGGCCTCCA GAAGACTGCA GACTTCGTGG AGGCCATCGA GGGGGCCCGG
    GCAGCGTGTG CCGAGGCCGG CCAGGCCGGG GTGCATGCCT ACCCCAGCGG CTCCCCCTTC
    CTCTTCTGGG AGCAGTACCT GAGCCTGCGG CGCTGCTTCC TGCTGGCGGT CTGCATCCTG
    CTGCTGTGTA CTTTCCTCGT CTGTGCCCTG CTGCTGCTCA ACCCCTGGAC GGCTGTGCTC
    ATAGTACTGG TCCTGGCGAT GATGACTGTG GAGCTCTTTG GCATCATGGG TTTCCTGGGC
    ATCAAGCTGA GTGCCATCCC GGTGGTGATT CTTGTGGCCT CCATAGGCAT CGGTGTCGAG
    TTCACTGTCC ACGTGGCTCT GGGCTTCCTG ACCGCTCAGG GTAGCCGGAA CCTGCGGGCT
    GCCCGGGCCC TGGAGCGCAC ATTTGCCCCA GTGACCGATG GGGCCATCTC CACGTTGCTA
    GGTCTGCTCA TGCTTGCTGG CTCCAACTTT GACTTCATCG TAAGGTACTT CTTTGTGGTG
    CTGACAATAC TTACACTCCT GGGCCTCCTC CACGGGCTCG TGCTGCTGCC GGTACTGCTG
    TCCATCCTGG GCCCCCCACC AGAGGTGGTA CAGATGTACA AGGAGAGCAC AGAGGTCCTG
    AGCCCCCCAG CTCCACAGGG AGGAGGGCTT AGGTGGGGGG TACCCTCCAC CCTGCCCCAG
    AGCTTTGCCA GAGTGACTAC CTCCATGACT GTGGCCCTCC ACCCACCCCC ACTGCCTGGT
    GCCTACATCC ACCCAGCCTC TGAGGAGCCT ACTTGGTCAC CTGCTGCCAC ACCAGCTGCC
    AATGGCCCCA GCAACCTCGG GTCTAGGGGA CTGTGTCCAG CCACGGGGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu10279
    Clone ID Related Accession (Same CDS sequence) XM_006052577.1
    Accession Version XM_006052577.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3750bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2014-03-11
    Organism Bubalus bubalis(water buffalo)
    Product protein patched homolog 2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005784468.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCTCGGC CGCCACCTCT CGGGGAGCTG CCCCCGGGCT ACACACCCCC AGCTCGACCT 
    GCAACACCCC AGATCCTAGC TGGGAGCCTG AAGGCTCCGC TCTGGCTCCG TGCTTACTTC
    CAGGGCCTGC TCTTCTCTTT GGGCTGCGGG ATCCAGAGAC ACTGTGGCAA AGTGCTCTTC
    CTGGGACTGT TGGCCTTTGG GGCCCTAGCA CTGGGTCTCC GCGTGGCCAT CATTGAGACA
    GACCTAGAAC AGCTCTGGGT GGAAGTGGGC AGCCGGGTGA GCCAGGAACT GGAATACACC
    AAGGAGAAGC TGGGGGAGGA GGCTGCGTAT ACCTCCCAGA TGTTGATACA GACCCCGCGT
    CAGGAGGGGG AGAACGTCCT CACGCCCGAG GCACTTGACC TCCACCTCCA GGCAGCCCTC
    ACCGCCAGTA AAGTGCAAGT ATCCCTCTAT GGAAAGTCCT GGGATCTGAA CAAAATCTGC
    TACAAGTCAG GAGTTCCCCT AATTGAAAAT GGAATGATTG AGCGGATGAT TGAAAAGCTG
    TTTCCCTGCG TGATCCTCAC CCCCCTCGAC TGCTTCTGGG AGGGAGCCAA ACTCCAAGGG
    GGCTCTGCCT ACTTGCCGGG CCGCCCAGAC ATCCAGTGGA CCAACCTGGA CCCAGAGCAG
    CTGCTTGAGG AGCTGGGCCC CTTTGCCTCC CTTGAGGGCT TCCGGGAGCT GCTAGACAAG
    GCACAGGTGG GCCAGGCCTA CGTGGGTCGG CCCTGTCTGC ACCCTGACGA CCTCCACTGC
    CCACCTAGTG CCCCTAACCA TCACAGCAAG CAGGCTCCCA ACGTGGCTCA GGAGTTGAGT
    GGGGGCTGCC ATGGCTTCTC CCACAAGTTT ATGCACTGGC AGGAGGAGCT GCTGCTGGGA
    GGCATGGCCA GAGACCCCCA AGGACAGCTG CTGAGGGCAG AGGCCCTGCA GAGCACCTTC
    CTGCTAATGA GCCCCCGCCA GCTCTACGAG CACTTCCGAG GTGACTACCA GACGCATGAC
    ATCGGCTGGA GCGAGGAGCA GGCCGGCACG GTCCTTCAGG CCTGGCAGCG ACGCTTTGTG
    CAGCTGGCCC AGGAGGCCCT GCCCGAGAAC GCATCCCAGC AGATTCACGC CTTCTCCTCT
    ACCACCCTGG ATGACATCCT GCACGCCTTC TCTGAAGTCA GCGCTGCTCG CGTGGCGGGG
    GGCTACCTGC TCATGCTAGC CTATGCCTGT GTGACGATGC TGCGGTGGGA CTGTGCTCAG
    TCCCAGGGTG CAGTGGGCCT CGCTGGTGTG CTGCTGGTGG CCCTGGCAGT GGCCTCGGGC
    CTGGGGCTCT GTGCCCTGTT GGGCATCGCC TTCAACGCTG CCACTACCCA GGTGCTGCCT
    TTCTTGGCAC TGGGCATCGG CGTGGATGAC ATATTCCTGC TGGCACACGC CTTCACAGAG
    GCTCCACCTG GCACCCCTCT CCAGGAGCGC ACGGGGGAGT GTCTGCGGCG CACAGGCACC
    GGTGTCACAC TCACATCCGT CAACAATATG GTCGCCTTCT TCATGGCCGC CCTAGTCCCC
    ATCCCTGCAC TTCGGGCCTT CTCCTTGCAG GCAGCCATAG TGGTGGGCTG CAACTTTGCA
    GCCGTGATGC TTGTCTTCCC AGCGGTCCTC AGCCTGGACC TGCAGCGGCG CCACTGCCGG
    CGCCTGGATG TGCTCTGCTG CTTCTCTAGC CCCTGCTCTG CTCGGGTGAT TCAGATTCTG
    CCCCAGGAGC TGGGGAACGG GACGGTACCA GTGGGCGTTG CCCACCTGAC TGCCACGGTT
    CAAGCCTTTG CCCACTGTGA AGCCAGGAGC CAACATGTGG TCACCATCTT GCCTCCCCAA
    GCTCACCTGG TGCCGCCACC TTCTGACCCT TTGGGCTCTG AGCTCTTCAG CCCAGGAGGG
    TCCACACGGG ACCTTCTAGG GCAGGAGGAG GGGACAGGGC AGAAGGCAGC CTGCAAGTCC
    CTGCCCTGTG CCCGCTGGAA TCTTGCCCAT TTCGCCCGCT CTCAGTTTGC ACCCTTGTTG
    CTCCAGTCCC CCACCAAGGC TGTAGTACTG GTACTTTTTG GGGCTCTCCT GGGCCTGAGC
    CTCTATGGAG CGACCTTGGT GCAGGACGGT CTGGCCCTGA CTGATGTGGT GCCGCGTGGC
    ACCAAGGAGC ATGCCTTCCT GAGCGCCCAG CTCAGGTACT TCTCTCTGTA CGAGGTGGCT
    CTGGTGACAC AGGGTGGCTT TGACTACGCC CACTCCCAAC GCGCCCTCTT TGATCTGCAC
    CAGCGCTTCA GTTCCCTCAA GGCCGTGCTG CCCCCACCGG CCACCCAGGC ACCCCGCACC
    TGGCTGCACT ACTACCGCAG CTGGCTACAG GGAATCCAGG CTGCATTTGA CCAGGACTGG
    GCTTCTGGGC GCATCACCCG CCACTCATAC CGCAATGGCT CTGAGGATGG GGCCCTGGCA
    TACAAGCTGC TTGTCCAGAC CGGGGATGCC CAGGAGCCTC TAGATTTCAG CCAGCTGACC
    ACCAAGAAGC TGGTGGACAA GGAAGGGCTG ATCCCACCCG AGCTCTTCTA CATGGGGCTG
    ACCGTGTGGG TGAGCAGTGA CCCCCTGGGT CTGGCAGCCT CACAGGCCAA CTTCTATCCC
    CCACCTCCCG AGTGGCTGCA TGACAAGTAC GATACCACGG GGGAGAATCT TCGCAGTGAG
    TTCTGGGGGA GCCCGAACTG CCTGGGCCCA AGCCCACCCA GGCCCTCCCC TGAAGCCCTG
    CCCACTGCTG CCAGAGCTCA CCGGCCGCCC CTCCCTCTCC CTCCGACCCC TCCCTCCACA
    GTCCCGGCGG CCCAGCCCTT GGAGTTTGCC CAGTTCCCCT TCCTCCTGCG CGGCCTCCAG
    AAGACTGCAG ACTTCGTGGA GGCCATCGAG GGGGCCCGGG CAGCGTGTGC CGAGGCCGGC
    CAGGCCGGGG TGCATGCCTA CCCCAGCGGC TCCCCCTTCC TCTTCTGGGA GCAGTACCTG
    AGCCTGCGGC GCTGCTTCCT GCTGGCGGTC TGCATCCTGC TGCTGTGTAC TTTCCTCGTC
    TGTGCCCTGC TGCTGCTCAA CCCCTGGACG GCTGTGCTCA TAGTACTGGT CCTGGCGATG
    ATGACTGTGG AGCTCTTTGG CATCATGGGT TTCCTGGGCA TCAAGCTGAG TGCCATCCCG
    GTGGTGATTC TTGTGGCCTC CATAGGCATC GGTGTCGAGT TCACTGTCCA CGTGGCTCTG
    GGCTTCCTGA CCGCTCAGGG TAGCCGGAAC CTGCGGGCTG CCCGGGCCCT GGAGCGCACA
    TTTGCCCCAG TGACCGATGG GGCCATCTCC ACGTTGCTAG GTCTGCTCAT GCTTGCTGGC
    TCCAACTTTG ACTTCATCGT AAGGTACTTC TTTGTGGTGC TGACAATACT TACACTCCTG
    GGCCTCCTCC ACGGGCTCGT GCTGCTGCCG GTACTGCTGT CCATCCTGGG CCCCCCACCA
    GAGGTGGTAC AGATGTACAA GGAGAGCACA GAGGTCCTGA GCCCCCCAGC TCCACAGGGA
    GGAGGGCTTA GGTGGGGGGT ACCCTCCACC CTGCCCCAGA GCTTTGCCAG AGTGACTACC
    TCCATGACTG TGGCCCTCCA CCCACCCCCA CTGCCTGGTG CCTACATCCA CCCAGCCTCT
    GAGGAGCCTA CTTGGTCACC TGCTGCCACA CCAGCTGCCA ATGGCCCCAG CAACCTCGGG
    TCTAGGGGAC TGTGTCCAGC CACGGGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006052639.1
    CDS1799..5548
    Translation

    Target ORF information:

    RefSeq Version XM_006052577.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis patched 2 (PTCH2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006052577.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCTCGGC CGCCACCTCT CGGGGAGCTG CCCCCGGGCT ACACACCCCC AGCTCGACCT 
    GCAACACCCC AGATCCTAGC TGGGAGCCTG AAGGCTCCGC TCTGGCTCCG TGCTTACTTC
    CAGGGCCTGC TCTTCTCTTT GGGCTGCGGG ATCCAGAGAC ACTGTGGCAA AGTGCTCTTC
    CTGGGACTGT TGGCCTTTGG GGCCCTAGCA CTGGGTCTCC GCGTGGCCAT CATTGAGACA
    GACCTAGAAC AGCTCTGGGT GGAAGTGGGC AGCCGGGTGA GCCAGGAACT GGAATACACC
    AAGGAGAAGC TGGGGGAGGA GGCTGCGTAT ACCTCCCAGA TGTTGATACA GACCCCGCGT
    CAGGAGGGGG AGAACGTCCT CACGCCCGAG GCACTTGACC TCCACCTCCA GGCAGCCCTC
    ACCGCCAGTA AAGTGCAAGT ATCCCTCTAT GGAAAGTCCT GGGATCTGAA CAAAATCTGC
    TACAAGTCAG GAGTTCCCCT AATTGAAAAT GGAATGATTG AGCGGATGAT TGAAAAGCTG
    TTTCCCTGCG TGATCCTCAC CCCCCTCGAC TGCTTCTGGG AGGGAGCCAA ACTCCAAGGG
    GGCTCTGCCT ACTTGCCGGG CCGCCCAGAC ATCCAGTGGA CCAACCTGGA CCCAGAGCAG
    CTGCTTGAGG AGCTGGGCCC CTTTGCCTCC CTTGAGGGCT TCCGGGAGCT GCTAGACAAG
    GCACAGGTGG GCCAGGCCTA CGTGGGTCGG CCCTGTCTGC ACCCTGACGA CCTCCACTGC
    CCACCTAGTG CCCCTAACCA TCACAGCAAG CAGGCTCCCA ACGTGGCTCA GGAGTTGAGT
    GGGGGCTGCC ATGGCTTCTC CCACAAGTTT ATGCACTGGC AGGAGGAGCT GCTGCTGGGA
    GGCATGGCCA GAGACCCCCA AGGACAGCTG CTGAGGGCAG AGGCCCTGCA GAGCACCTTC
    CTGCTAATGA GCCCCCGCCA GCTCTACGAG CACTTCCGAG GTGACTACCA GACGCATGAC
    ATCGGCTGGA GCGAGGAGCA GGCCGGCACG GTCCTTCAGG CCTGGCAGCG ACGCTTTGTG
    CAGCTGGCCC AGGAGGCCCT GCCCGAGAAC GCATCCCAGC AGATTCACGC CTTCTCCTCT
    ACCACCCTGG ATGACATCCT GCACGCCTTC TCTGAAGTCA GCGCTGCTCG CGTGGCGGGG
    GGCTACCTGC TCATGCTAGC CTATGCCTGT GTGACGATGC TGCGGTGGGA CTGTGCTCAG
    TCCCAGGGTG CAGTGGGCCT CGCTGGTGTG CTGCTGGTGG CCCTGGCAGT GGCCTCGGGC
    CTGGGGCTCT GTGCCCTGTT GGGCATCGCC TTCAACGCTG CCACTACCCA GGTGCTGCCT
    TTCTTGGCAC TGGGCATCGG CGTGGATGAC ATATTCCTGC TGGCACACGC CTTCACAGAG
    GCTCCACCTG GCACCCCTCT CCAGGAGCGC ACGGGGGAGT GTCTGCGGCG CACAGGCACC
    GGTGTCACAC TCACATCCGT CAACAATATG GTCGCCTTCT TCATGGCCGC CCTAGTCCCC
    ATCCCTGCAC TTCGGGCCTT CTCCTTGCAG GCAGCCATAG TGGTGGGCTG CAACTTTGCA
    GCCGTGATGC TTGTCTTCCC AGCGGTCCTC AGCCTGGACC TGCAGCGGCG CCACTGCCGG
    CGCCTGGATG TGCTCTGCTG CTTCTCTAGC CCCTGCTCTG CTCGGGTGAT TCAGATTCTG
    CCCCAGGAGC TGGGGAACGG GACGGTACCA GTGGGCGTTG CCCACCTGAC TGCCACGGTT
    CAAGCCTTTG CCCACTGTGA AGCCAGGAGC CAACATGTGG TCACCATCTT GCCTCCCCAA
    GCTCACCTGG TGCCGCCACC TTCTGACCCT TTGGGCTCTG AGCTCTTCAG CCCAGGAGGG
    TCCACACGGG ACCTTCTAGG GCAGGAGGAG GGGACAGGGC AGAAGGCAGC CTGCAAGTCC
    CTGCCCTGTG CCCGCTGGAA TCTTGCCCAT TTCGCCCGCT CTCAGTTTGC ACCCTTGTTG
    CTCCAGTCCC CCACCAAGGC TGTAGTACTG GTACTTTTTG GGGCTCTCCT GGGCCTGAGC
    CTCTATGGAG CGACCTTGGT GCAGGACGGT CTGGCCCTGA CTGATGTGGT GCCGCGTGGC
    ACCAAGGAGC ATGCCTTCCT GAGCGCCCAG CTCAGGTACT TCTCTCTGTA CGAGGTGGCT
    CTGGTGACAC AGGGTGGCTT TGACTACGCC CACTCCCAAC GCGCCCTCTT TGATCTGCAC
    CAGCGCTTCA GTTCCCTCAA GGCCGTGCTG CCCCCACCGG CCACCCAGGC ACCCCGCACC
    TGGCTGCACT ACTACCGCAG CTGGCTACAG GGAATCCAGG CTGCATTTGA CCAGGACTGG
    GCTTCTGGGC GCATCACCCG CCACTCATAC CGCAATGGCT CTGAGGATGG GGCCCTGGCA
    TACAAGCTGC TTGTCCAGAC CGGGGATGCC CAGGAGCCTC TAGATTTCAG CCAGCTGACC
    ACCAAGAAGC TGGTGGACAA GGAAGGGCTG ATCCCACCCG AGCTCTTCTA CATGGGGCTG
    ACCGTGTGGG TGAGCAGTGA CCCCCTGGGT CTGGCAGCCT CACAGGCCAA CTTCTATCCC
    CCACCTCCCG AGTGGCTGCA TGACAAGTAC GATACCACGG GGGAGAATCT TCGCAGTGAG
    TTCTGGGGGA GCCCGAACTG CCTGGGCCCA AGCCCACCCA GGCCCTCCCC TGAAGCCCTG
    CCCACTGCTG CCAGAGCTCA CCGGCCGCCC CTCCCTCTCC CTCCGACCCC TCCCTCCACA
    GTCCCGGCGG CCCAGCCCTT GGAGTTTGCC CAGTTCCCCT TCCTCCTGCG CGGCCTCCAG
    AAGACTGCAG ACTTCGTGGA GGCCATCGAG GGGGCCCGGG CAGCGTGTGC CGAGGCCGGC
    CAGGCCGGGG TGCATGCCTA CCCCAGCGGC TCCCCCTTCC TCTTCTGGGA GCAGTACCTG
    AGCCTGCGGC GCTGCTTCCT GCTGGCGGTC TGCATCCTGC TGCTGTGTAC TTTCCTCGTC
    TGTGCCCTGC TGCTGCTCAA CCCCTGGACG GCTGTGCTCA TAGTACTGGT CCTGGCGATG
    ATGACTGTGG AGCTCTTTGG CATCATGGGT TTCCTGGGCA TCAAGCTGAG TGCCATCCCG
    GTGGTGATTC TTGTGGCCTC CATAGGCATC GGTGTCGAGT TCACTGTCCA CGTGGCTCTG
    GGCTTCCTGA CCGCTCAGGG TAGCCGGAAC CTGCGGGCTG CCCGGGCCCT GGAGCGCACA
    TTTGCCCCAG TGACCGATGG GGCCATCTCC ACGTTGCTAG GTCTGCTCAT GCTTGCTGGC
    TCCAACTTTG ACTTCATCGT AAGGTACTTC TTTGTGGTGC TGACAATACT TACACTCCTG
    GGCCTCCTCC ACGGGCTCGT GCTGCTGCCG GTACTGCTGT CCATCCTGGG CCCCCCACCA
    GAGGTGGTAC AGATGTACAA GGAGAGCACA GAGGTCCTGA GCCCCCCAGC TCCACAGGGA
    GGAGGGCTTA GGTGGGGGGT ACCCTCCACC CTGCCCCAGA GCTTTGCCAG AGTGACTACC
    TCCATGACTG TGGCCCTCCA CCCACCCCCA CTGCCTGGTG CCTACATCCA CCCAGCCTCT
    GAGGAGCCTA CTTGGTCACC TGCTGCCACA CCAGCTGCCA ATGGCCCCAG CAACCTCGGG
    TCTAGGGGAC TGTGTCCAGC CACGGGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu10280
    Clone ID Related Accession (Same CDS sequence) XM_006052578.1
    Accession Version XM_006052578.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3750bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2014-03-11
    Organism Bubalus bubalis(water buffalo)
    Product protein patched homolog 2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005784468.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCTCGGC CGCCACCTCT CGGGGAGCTG CCCCCGGGCT ACACACCCCC AGCTCGACCT 
    GCAACACCCC AGATCCTAGC TGGGAGCCTG AAGGCTCCGC TCTGGCTCCG TGCTTACTTC
    CAGGGCCTGC TCTTCTCTTT GGGCTGCGGG ATCCAGAGAC ACTGTGGCAA AGTGCTCTTC
    CTGGGACTGT TGGCCTTTGG GGCCCTAGCA CTGGGTCTCC GCGTGGCCAT CATTGAGACA
    GACCTAGAAC AGCTCTGGGT GGAAGTGGGC AGCCGGGTGA GCCAGGAACT GGAATACACC
    AAGGAGAAGC TGGGGGAGGA GGCTGCGTAT ACCTCCCAGA TGTTGATACA GACCCCGCGT
    CAGGAGGGGG AGAACGTCCT CACGCCCGAG GCACTTGACC TCCACCTCCA GGCAGCCCTC
    ACCGCCAGTA AAGTGTCCTG GGATCTGAAC AAAATCTGCT ACAAGTCAGG AGTTCCCCTA
    ATTGAAAATG GAATGATTGA GCGGATGATT GAAAAGCTGT TTCCCTGCGT GATCCTCACC
    CCCCTCGACT GCTTCTGGGA GGGAGCCAAA CTCCAAGGGG GCTCTGCCTA CTTGCCGGGC
    CGCCCAGACA TCCAGTGGAC CAACCTGGAC CCAGAGCAGC TGCTTGAGGA GCTGGGCCCC
    TTTGCCTCCC TTGAGGGCTT CCGGGAGCTG CTAGACAAGG CACAGGTGGG CCAGGCCTAC
    GTGGGTCGGC CCTGTCTGCA CCCTGACGAC CTCCACTGCC CACCTAGTGC CCCTAACCAT
    CACAGCAAGC AGGCTCCCAA CGTGGCTCAG GAGTTGAGTG GGGGCTGCCA TGGCTTCTCC
    CACAAGTTTA TGCACTGGCA GGAGGAGCTG CTGCTGGGAG GCATGGCCAG AGACCCCCAA
    GGACAGCTGC TGAGGGCAGA GGCCCTGCAG AGCACCTTCC TGCTAATGAG CCCCCGCCAG
    CTCTACGAGC ACTTCCGAGG TGACTACCAG ACGCATGACA TCGGCTGGAG CGAGGAGCAG
    GCCGGCACGG TCCTTCAGGC CTGGCAGCGA CGCTTTGTGC AGCTGGCCCA GGAGGCCCTG
    CCCGAGAACG CATCCCAGCA GATTCACGCC TTCTCCTCTA CCACCCTGGA TGACATCCTG
    CACGCCTTCT CTGAAGTCAG CGCTGCTCGC GTGGCGGGGG GCTACCTGCT CATGCTAGCC
    TATGCCTGTG TGACGATGCT GCGGTGGGAC TGTGCTCAGT CCCAGGGTGC AGTGGGCCTC
    GCTGGTGTGC TGCTGGTGGC CCTGGCAGTG GCCTCGGGCC TGGGGCTCTG TGCCCTGTTG
    GGCATCGCCT TCAACGCTGC CACTACCCAG GTGCTGCCTT TCTTGGCACT GGGCATCGGC
    GTGGATGACA TATTCCTGCT GGCACACGCC TTCACAGAGG CTCCACCTGG CACCCCTCTC
    CAGGAGCGCA CGGGGGAGTG TCTGCGGCGC ACAGGCACCG GTGTCACACT CACATCCGTC
    AACAATATGG TCGCCTTCTT CATGGCCGCC CTAGTCCCCA TCCCTGCACT TCGGGCCTTC
    TCCTTGCAGG CAGCCATAGT GGTGGGCTGC AACTTTGCAG CCGTGATGCT TGTCTTCCCA
    GCGGTCCTCA GCCTGGACCT GCAGCGGCGC CACTGCCGGC GCCTGGATGT GCTCTGCTGC
    TTCTCTAGCC CCTGCTCTGC TCGGGTGATT CAGATTCTGC CCCAGGAGCT GGGGAACGGG
    ACGGTACCAG TGGGCGTTGC CCACCTGACT GCCACGGTTC AAGCCTTTGC CCACTGTGAA
    GCCAGGAGCC AACATGTGGT CACCATCTTG CCTCCCCAAG CTCACCTGGT GCCGCCACCT
    TCTGACCCTT TGGGCTCTGA GCTCTTCAGC CCAGGAGGGT CCACACGGGA CCTTCTAGGG
    CAGGAGGAGG GGACAGGGCA GAAGGCAGCC TGCAAGTCCC TGCCCTGTGC CCGCTGGAAT
    CTTGCCCATT TCGCCCGCTC TCAGTTTGCA CCCTTGTTGC TCCAGTCCCC CACCAAGCTT
    AGTCACCTTT CCCCACAGGC TGTAGTACTG GTACTTTTTG GGGCTCTCCT GGGCCTGAGC
    CTCTATGGAG CGACCTTGGT GCAGGACGGT CTGGCCCTGA CTGATGTGGT GCCGCGTGGC
    ACCAAGGAGC ATGCCTTCCT GAGCGCCCAG CTCAGGTACT TCTCTCTGTA CGAGGTGGCT
    CTGGTGACAC AGGGTGGCTT TGACTACGCC CACTCCCAAC GCGCCCTCTT TGATCTGCAC
    CAGCGCTTCA GTTCCCTCAA GGCCGTGCTG CCCCCACCGG CCACCCAGGC ACCCCGCACC
    TGGCTGCACT ACTACCGCAG CTGGCTACAG GGAATCCAGG CTGCATTTGA CCAGGACTGG
    GCTTCTGGGC GCATCACCCG CCACTCATAC CGCAATGGCT CTGAGGATGG GGCCCTGGCA
    TACAAGCTGC TTGTCCAGAC CGGGGATGCC CAGGAGCCTC TAGATTTCAG CCAGCTGACC
    ACCAAGAAGC TGGTGGACAA GGAAGGGCTG ATCCCACCCG AGCTCTTCTA CATGGGGCTG
    ACCGTGTGGG TGAGCAGTGA CCCCCTGGGT CTGGCAGCCT CACAGGCCAA CTTCTATCCC
    CCACCTCCCG AGTGGCTGCA TGACAAGTAC GATACCACGG GGGAGAATCT TCGCAGTGAG
    TTCTGGGGGA GCCCGAACTG CCTGGGCCCA AGCCCACCCA GGCCCTCCCC TGAAGCCCTG
    CCCACTGCTG CCAGAGCTCA CCGGCCGCCC CTCCCTCTCC CTCCGACCCC TCCCTCCACA
    GTCCCGGCGG CCCAGCCCTT GGAGTTTGCC CAGTTCCCCT TCCTCCTGCG CGGCCTCCAG
    AAGACTGCAG ACTTCGTGGA GGCCATCGAG GGGGCCCGGG CAGCGTGTGC CGAGGCCGGC
    CAGGCCGGGG TGCATGCCTA CCCCAGCGGC TCCCCCTTCC TCTTCTGGGA GCAGTACCTG
    AGCCTGCGGC GCTGCTTCCT GCTGGCGGTC TGCATCCTGC TGCTGTGTAC TTTCCTCGTC
    TGTGCCCTGC TGCTGCTCAA CCCCTGGACG GCTGTGCTCA TAGTACTGGT CCTGGCGATG
    ATGACTGTGG AGCTCTTTGG CATCATGGGT TTCCTGGGCA TCAAGCTGAG TGCCATCCCG
    GTGGTGATTC TTGTGGCCTC CATAGGCATC GGTGTCGAGT TCACTGTCCA CGTGGCTCTG
    GGCTTCCTGA CCGCTCAGGG TAGCCGGAAC CTGCGGGCTG CCCGGGCCCT GGAGCGCACA
    TTTGCCCCAG TGACCGATGG GGCCATCTCC ACGTTGCTAG GTCTGCTCAT GCTTGCTGGC
    TCCAACTTTG ACTTCATCGT AAGGTACTTC TTTGTGGTGC TGACAATACT TACACTCCTG
    GGCCTCCTCC ACGGGCTCGT GCTGCTGCCG GTACTGCTGT CCATCCTGGG CCCCCCACCA
    GAGGTGGTAC AGATGTACAA GGAGAGCACA GAGGTCCTGA GCCCCCCAGC TCCACAGGGA
    GGAGGGCTTA GGTGGGGGGT ACCCTCCACC CTGCCCCAGA GCTTTGCCAG AGTGACTACC
    TCCATGACTG TGGCCCTCCA CCCACCCCCA CTGCCTGGTG CCTACATCCA CCCAGCCTCT
    GAGGAGCCTA CTTGGTCACC TGCTGCCACA CCAGCTGCCA ATGGCCCCAG CAACCTCGGG
    TCTAGGGGAC TGTGTCCAGC CACGGGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006052640.1
    CDS1801..5550
    Translation

    Target ORF information:

    RefSeq Version XM_006052578.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis patched 2 (PTCH2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006052578.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCTCGGC CGCCACCTCT CGGGGAGCTG CCCCCGGGCT ACACACCCCC AGCTCGACCT 
    GCAACACCCC AGATCCTAGC TGGGAGCCTG AAGGCTCCGC TCTGGCTCCG TGCTTACTTC
    CAGGGCCTGC TCTTCTCTTT GGGCTGCGGG ATCCAGAGAC ACTGTGGCAA AGTGCTCTTC
    CTGGGACTGT TGGCCTTTGG GGCCCTAGCA CTGGGTCTCC GCGTGGCCAT CATTGAGACA
    GACCTAGAAC AGCTCTGGGT GGAAGTGGGC AGCCGGGTGA GCCAGGAACT GGAATACACC
    AAGGAGAAGC TGGGGGAGGA GGCTGCGTAT ACCTCCCAGA TGTTGATACA GACCCCGCGT
    CAGGAGGGGG AGAACGTCCT CACGCCCGAG GCACTTGACC TCCACCTCCA GGCAGCCCTC
    ACCGCCAGTA AAGTGTCCTG GGATCTGAAC AAAATCTGCT ACAAGTCAGG AGTTCCCCTA
    ATTGAAAATG GAATGATTGA GCGGATGATT GAAAAGCTGT TTCCCTGCGT GATCCTCACC
    CCCCTCGACT GCTTCTGGGA GGGAGCCAAA CTCCAAGGGG GCTCTGCCTA CTTGCCGGGC
    CGCCCAGACA TCCAGTGGAC CAACCTGGAC CCAGAGCAGC TGCTTGAGGA GCTGGGCCCC
    TTTGCCTCCC TTGAGGGCTT CCGGGAGCTG CTAGACAAGG CACAGGTGGG CCAGGCCTAC
    GTGGGTCGGC CCTGTCTGCA CCCTGACGAC CTCCACTGCC CACCTAGTGC CCCTAACCAT
    CACAGCAAGC AGGCTCCCAA CGTGGCTCAG GAGTTGAGTG GGGGCTGCCA TGGCTTCTCC
    CACAAGTTTA TGCACTGGCA GGAGGAGCTG CTGCTGGGAG GCATGGCCAG AGACCCCCAA
    GGACAGCTGC TGAGGGCAGA GGCCCTGCAG AGCACCTTCC TGCTAATGAG CCCCCGCCAG
    CTCTACGAGC ACTTCCGAGG TGACTACCAG ACGCATGACA TCGGCTGGAG CGAGGAGCAG
    GCCGGCACGG TCCTTCAGGC CTGGCAGCGA CGCTTTGTGC AGCTGGCCCA GGAGGCCCTG
    CCCGAGAACG CATCCCAGCA GATTCACGCC TTCTCCTCTA CCACCCTGGA TGACATCCTG
    CACGCCTTCT CTGAAGTCAG CGCTGCTCGC GTGGCGGGGG GCTACCTGCT CATGCTAGCC
    TATGCCTGTG TGACGATGCT GCGGTGGGAC TGTGCTCAGT CCCAGGGTGC AGTGGGCCTC
    GCTGGTGTGC TGCTGGTGGC CCTGGCAGTG GCCTCGGGCC TGGGGCTCTG TGCCCTGTTG
    GGCATCGCCT TCAACGCTGC CACTACCCAG GTGCTGCCTT TCTTGGCACT GGGCATCGGC
    GTGGATGACA TATTCCTGCT GGCACACGCC TTCACAGAGG CTCCACCTGG CACCCCTCTC
    CAGGAGCGCA CGGGGGAGTG TCTGCGGCGC ACAGGCACCG GTGTCACACT CACATCCGTC
    AACAATATGG TCGCCTTCTT CATGGCCGCC CTAGTCCCCA TCCCTGCACT TCGGGCCTTC
    TCCTTGCAGG CAGCCATAGT GGTGGGCTGC AACTTTGCAG CCGTGATGCT TGTCTTCCCA
    GCGGTCCTCA GCCTGGACCT GCAGCGGCGC CACTGCCGGC GCCTGGATGT GCTCTGCTGC
    TTCTCTAGCC CCTGCTCTGC TCGGGTGATT CAGATTCTGC CCCAGGAGCT GGGGAACGGG
    ACGGTACCAG TGGGCGTTGC CCACCTGACT GCCACGGTTC AAGCCTTTGC CCACTGTGAA
    GCCAGGAGCC AACATGTGGT CACCATCTTG CCTCCCCAAG CTCACCTGGT GCCGCCACCT
    TCTGACCCTT TGGGCTCTGA GCTCTTCAGC CCAGGAGGGT CCACACGGGA CCTTCTAGGG
    CAGGAGGAGG GGACAGGGCA GAAGGCAGCC TGCAAGTCCC TGCCCTGTGC CCGCTGGAAT
    CTTGCCCATT TCGCCCGCTC TCAGTTTGCA CCCTTGTTGC TCCAGTCCCC CACCAAGCTT
    AGTCACCTTT CCCCACAGGC TGTAGTACTG GTACTTTTTG GGGCTCTCCT GGGCCTGAGC
    CTCTATGGAG CGACCTTGGT GCAGGACGGT CTGGCCCTGA CTGATGTGGT GCCGCGTGGC
    ACCAAGGAGC ATGCCTTCCT GAGCGCCCAG CTCAGGTACT TCTCTCTGTA CGAGGTGGCT
    CTGGTGACAC AGGGTGGCTT TGACTACGCC CACTCCCAAC GCGCCCTCTT TGATCTGCAC
    CAGCGCTTCA GTTCCCTCAA GGCCGTGCTG CCCCCACCGG CCACCCAGGC ACCCCGCACC
    TGGCTGCACT ACTACCGCAG CTGGCTACAG GGAATCCAGG CTGCATTTGA CCAGGACTGG
    GCTTCTGGGC GCATCACCCG CCACTCATAC CGCAATGGCT CTGAGGATGG GGCCCTGGCA
    TACAAGCTGC TTGTCCAGAC CGGGGATGCC CAGGAGCCTC TAGATTTCAG CCAGCTGACC
    ACCAAGAAGC TGGTGGACAA GGAAGGGCTG ATCCCACCCG AGCTCTTCTA CATGGGGCTG
    ACCGTGTGGG TGAGCAGTGA CCCCCTGGGT CTGGCAGCCT CACAGGCCAA CTTCTATCCC
    CCACCTCCCG AGTGGCTGCA TGACAAGTAC GATACCACGG GGGAGAATCT TCGCAGTGAG
    TTCTGGGGGA GCCCGAACTG CCTGGGCCCA AGCCCACCCA GGCCCTCCCC TGAAGCCCTG
    CCCACTGCTG CCAGAGCTCA CCGGCCGCCC CTCCCTCTCC CTCCGACCCC TCCCTCCACA
    GTCCCGGCGG CCCAGCCCTT GGAGTTTGCC CAGTTCCCCT TCCTCCTGCG CGGCCTCCAG
    AAGACTGCAG ACTTCGTGGA GGCCATCGAG GGGGCCCGGG CAGCGTGTGC CGAGGCCGGC
    CAGGCCGGGG TGCATGCCTA CCCCAGCGGC TCCCCCTTCC TCTTCTGGGA GCAGTACCTG
    AGCCTGCGGC GCTGCTTCCT GCTGGCGGTC TGCATCCTGC TGCTGTGTAC TTTCCTCGTC
    TGTGCCCTGC TGCTGCTCAA CCCCTGGACG GCTGTGCTCA TAGTACTGGT CCTGGCGATG
    ATGACTGTGG AGCTCTTTGG CATCATGGGT TTCCTGGGCA TCAAGCTGAG TGCCATCCCG
    GTGGTGATTC TTGTGGCCTC CATAGGCATC GGTGTCGAGT TCACTGTCCA CGTGGCTCTG
    GGCTTCCTGA CCGCTCAGGG TAGCCGGAAC CTGCGGGCTG CCCGGGCCCT GGAGCGCACA
    TTTGCCCCAG TGACCGATGG GGCCATCTCC ACGTTGCTAG GTCTGCTCAT GCTTGCTGGC
    TCCAACTTTG ACTTCATCGT AAGGTACTTC TTTGTGGTGC TGACAATACT TACACTCCTG
    GGCCTCCTCC ACGGGCTCGT GCTGCTGCCG GTACTGCTGT CCATCCTGGG CCCCCCACCA
    GAGGTGGTAC AGATGTACAA GGAGAGCACA GAGGTCCTGA GCCCCCCAGC TCCACAGGGA
    GGAGGGCTTA GGTGGGGGGT ACCCTCCACC CTGCCCCAGA GCTTTGCCAG AGTGACTACC
    TCCATGACTG TGGCCCTCCA CCCACCCCCA CTGCCTGGTG CCTACATCCA CCCAGCCTCT
    GAGGAGCCTA CTTGGTCACC TGCTGCCACA CCAGCTGCCA ATGGCCCCAG CAACCTCGGG
    TCTAGGGGAC TGTGTCCAGC CACGGGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu10281
    Clone ID Related Accession (Same CDS sequence) XM_006052579.1
    Accession Version XM_006052579.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3645bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2014-03-11
    Organism Bubalus bubalis(water buffalo)
    Product protein patched homolog 2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005784468.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCTCGGC CGCCACCTCT CGGGGAGCTG CCCCCGGGCT ACACACCCCC AGCTCGACCT 
    GCAACACCCC AGATCCTAGC TGGGAGCCTG AAGGCTCCGC TCTGGCTCCG TGCTTACTTC
    CAGGGCCTGC TCTTCTCTTT GGGCTGCGGG ATCCAGAGAC ACTGTGGCAA AGTGCTCTTC
    CTGGGACTGT TGGCCTTTGG GGCCCTAGCA CTGGGTCTCC GCGTGGCCAT CATTGAGACA
    GACCTAGAAC AGCTCTGGGT GGAAGTGGGC AGCCGGGTGA GCCAGGAACT GGAATACACC
    AAGGAGAAGC TGGGGGAGGA GGCTGCGTAT ACCTCCCAGA TGTTGATACA GACCCCGCGT
    CAGGAGGGGG AGAACGTCCT CACGCCCGAG GCACTTGACC TCCACCTCCA GGCAGCCCTC
    ACCGCCAGTA AAGTGCAAGT ATCCCTCTAT GGAAAGTCCT GGGATCTGAA CAAAATCTGC
    TACAAGTCAG GAGTTCCCCT AATTGAAAAT GGAATGATTG AGCGGATGAT TGAAAAGCTG
    TTTCCCTGCG TGATCCTCAC CCCCCTCGAC TGCTTCTGGG AGGGAGCCAA ACTCCAAGGG
    GGCTCTGCCT ACTTGCCGGG CCGCCCAGAC ATCCAGTGGA CCAACCTGGA CCCAGAGCAG
    CTGCTTGAGG AGCTGGGCCC CTTTGCCTCC CTTGAGGGCT TCCGGGAGCT GCTAGACAAG
    GCACAGGTGG GCCAGGCCTA CGTGGGTCGG CCCTGTCTGC ACCCTGACGA CCTCCACTGC
    CCACCTAGTG CCCCTAACCA TCACAGCAAG CAGGCTCCCA ACGTGGCTCA GGAGTTGAGT
    GGGGGCTGCC ATGGCTTCTC CCACAAGTTT ATGCACTGGC AGGAGGAGCT GCTGCTGGGA
    GGCATGGCCA GAGACCCCCA AGGACAGCTG CTGAGGGCAG AGGCCCTGCA GAGCACCTTC
    CTGCTAATGA GCCCCCGCCA GCTCTACGAG CACTTCCGAG GTGACTACCA GACGCATGAC
    ATCGGCTGGA GCGAGGAGCA GGCCGGCACG GTCCTTCAGG CCTGGCAGCG ACGCTTTGTG
    CAGCTGGCCC AGGAGGCCCT GCCCGAGAAC GCATCCCAGC AGATTCACGC CTTCTCCTCT
    ACCACCCTGG ATGACATCCT GCACGCCTTC TCTGAAGTCA GCGCTGCTCG CGTGGCGGGG
    GGCTACCTGC TCATGCTAGC CTATGCCTGT GTGACGATGC TGCGGTGGGA CTGTGCTCAG
    TCCCAGGGTG CAGTGGGCCT CGCTGGTGTG CTGCTGGTGG CCCTGGCAGT GGCCTCGGGC
    CTGGGGCTCT GTGCCCTGTT GGGCATCGCC TTCAACGCTG CCACTACCCA GGTGCTGCCT
    TTCTTGGCAC TGGGCATCGG CGTGGATGAC ATATTCCTGC TGGCACACGC CTTCACAGAG
    GCTCCACCTG GCACCCCTCT CCAGGAGCGC ACGGGGGAGT GTCTGCGGCG CACAGGCACC
    GGTGTCACAC TCACATCCGT CAACAATATG GTCGCCTTCT TCATGGCCGC CCTAGTCCCC
    ATCCCTGCAC TTCGGGCCTT CTCCTTGCAG GCAGCCATAG TGGTGGGCTG CAACTTTGCA
    GCCGTGATGC TTGTCTTCCC AGCGGTCCTC AGCCTGGACC TGCAGCGGCG CCACTGCCGG
    CGCCTGGATG TGCTCTGCTG CTTCTCTAGC CCCTGCTCTG CTCGGGTGAT TCAGATTCTG
    CCCCAGGAGC TGGGGAACGG GACGGTACCA GTGGGCGTTG CCCACCTGAC TGCCACGGTT
    CAAGCCTTTG CCCACTGTGA AGCCAGGAGC CAACATGTGG TCACCATCTT GCCTCCCCAA
    GCTCACCTGG TGCCGCCACC TTCTGACCCT TTGGGCTCTG AGCTCTTCAG CCCAGGAGGG
    TCCACACGGG ACCTTCTAGG GCAGGAGGAG GGGACAGGGC AGAAGGCAGC CTGCAAGTCC
    CTGCCCTGTG CCCGCTGGAA TCTTGCCCAT TTCGCCCGCT CTCAGTTTGC ACCCTTGTTG
    CTCCAGTCCC CCACCAAGCT TAGTCACCTT TCCCCACAGG CTGTAGTACT GGTACTTTTT
    GGGGCTCTCC TGGGCCTGAG CCTCTATGGA GCGACCTTGG TGCAGGACGG TCTGGCCCTG
    ACTGATGTGG TGCCGCGTGG CACCAAGGAG CATGCCTTCC TGAGCGCCCA GCTCAGGTAC
    TTCTCTCTGT ACGAGGTGGC TCTGGTGACA CAGGGTGGCT TTGACTACGC CCACTCCCAA
    CGCGCCCTCT TTGATCTGCA CCAGCGCTTC AGTTCCCTCA AGGCCGTGCT GCCCCCACCG
    GCCACCCAGG CACCCCGCAC CTGGCTGCAC TACTACCGCA GCTGGCTACA GGGAATCCAG
    GCTGCATTTG ACCAGGACTG GGCTTCTGGG CGCATCACCC GCCACTCATA CCGCAATGGC
    TCTGAGGATG GGGCCCTGGC ATACAAGCTG CTTGTCCAGA CCGGGGATGC CCAGGAGCCT
    CTAGATTTCA GCCAGCTGAC CACCAAGAAG CTGGTGGACA AGGAAGGGCT GATCCCACCC
    GAGCTCTTCT ACATGGGGCT GACCGTGTGG GTGAGCAGTG ACCCCCTGGG TCTGGCAGCC
    TCACAGGCCA ACTTCTATCC CCCACCTCCC GAGTGGCTGC ATGACAAGTA CGATACCACG
    GGGGAGAATC TTCGCATCCC GGCGGCCCAG CCCTTGGAGT TTGCCCAGTT CCCCTTCCTC
    CTGCGCGGCC TCCAGAAGAC TGCAGACTTC GTGGAGGCCA TCGAGGGGGC CCGGGCAGCG
    TGTGCCGAGG CCGGCCAGGC CGGGGTGCAT GCCTACCCCA GCGGCTCCCC CTTCCTCTTC
    TGGGAGCAGT ACCTGAGCCT GCGGCGCTGC TTCCTGCTGG CGGTCTGCAT CCTGCTGCTG
    TGTACTTTCC TCGTCTGTGC CCTGCTGCTG CTCAACCCCT GGACGGCTGT GCTCATAGTA
    CTGGTCCTGG CGATGATGAC TGTGGAGCTC TTTGGCATCA TGGGTTTCCT GGGCATCAAG
    CTGAGTGCCA TCCCGGTGGT GATTCTTGTG GCCTCCATAG GCATCGGTGT CGAGTTCACT
    GTCCACGTGG CTCTGGGCTT CCTGACCGCT CAGGGTAGCC GGAACCTGCG GGCTGCCCGG
    GCCCTGGAGC GCACATTTGC CCCAGTGACC GATGGGGCCA TCTCCACGTT GCTAGGTCTG
    CTCATGCTTG CTGGCTCCAA CTTTGACTTC ATCGTAAGGT ACTTCTTTGT GGTGCTGACA
    ATACTTACAC TCCTGGGCCT CCTCCACGGG CTCGTGCTGC TGCCGGTACT GCTGTCCATC
    CTGGGCCCCC CACCAGAGGT GGTACAGATG TACAAGGAGA GCACAGAGGT CCTGAGCCCC
    CCAGCTCCAC AGGGAGGAGG GCTTAGGTGG GGGGTACCCT CCACCCTGCC CCAGAGCTTT
    GCCAGAGTGA CTACCTCCAT GACTGTGGCC CTCCACCCAC CCCCACTGCC TGGTGCCTAC
    ATCCACCCAG CCTCTGAGGA GCCTACTTGG TCACCTGCTG CCACACCAGC TGCCAATGGC
    CCCAGCAACC TCGGGTCTAG GGGACTGTGT CCAGCCACGG GGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006052641.1
    CDS1798..5442
    Translation

    Target ORF information:

    RefSeq Version XM_006052579.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis patched 2 (PTCH2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006052579.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCTCGGC CGCCACCTCT CGGGGAGCTG CCCCCGGGCT ACACACCCCC AGCTCGACCT 
    GCAACACCCC AGATCCTAGC TGGGAGCCTG AAGGCTCCGC TCTGGCTCCG TGCTTACTTC
    CAGGGCCTGC TCTTCTCTTT GGGCTGCGGG ATCCAGAGAC ACTGTGGCAA AGTGCTCTTC
    CTGGGACTGT TGGCCTTTGG GGCCCTAGCA CTGGGTCTCC GCGTGGCCAT CATTGAGACA
    GACCTAGAAC AGCTCTGGGT GGAAGTGGGC AGCCGGGTGA GCCAGGAACT GGAATACACC
    AAGGAGAAGC TGGGGGAGGA GGCTGCGTAT ACCTCCCAGA TGTTGATACA GACCCCGCGT
    CAGGAGGGGG AGAACGTCCT CACGCCCGAG GCACTTGACC TCCACCTCCA GGCAGCCCTC
    ACCGCCAGTA AAGTGCAAGT ATCCCTCTAT GGAAAGTCCT GGGATCTGAA CAAAATCTGC
    TACAAGTCAG GAGTTCCCCT AATTGAAAAT GGAATGATTG AGCGGATGAT TGAAAAGCTG
    TTTCCCTGCG TGATCCTCAC CCCCCTCGAC TGCTTCTGGG AGGGAGCCAA ACTCCAAGGG
    GGCTCTGCCT ACTTGCCGGG CCGCCCAGAC ATCCAGTGGA CCAACCTGGA CCCAGAGCAG
    CTGCTTGAGG AGCTGGGCCC CTTTGCCTCC CTTGAGGGCT TCCGGGAGCT GCTAGACAAG
    GCACAGGTGG GCCAGGCCTA CGTGGGTCGG CCCTGTCTGC ACCCTGACGA CCTCCACTGC
    CCACCTAGTG CCCCTAACCA TCACAGCAAG CAGGCTCCCA ACGTGGCTCA GGAGTTGAGT
    GGGGGCTGCC ATGGCTTCTC CCACAAGTTT ATGCACTGGC AGGAGGAGCT GCTGCTGGGA
    GGCATGGCCA GAGACCCCCA AGGACAGCTG CTGAGGGCAG AGGCCCTGCA GAGCACCTTC
    CTGCTAATGA GCCCCCGCCA GCTCTACGAG CACTTCCGAG GTGACTACCA GACGCATGAC
    ATCGGCTGGA GCGAGGAGCA GGCCGGCACG GTCCTTCAGG CCTGGCAGCG ACGCTTTGTG
    CAGCTGGCCC AGGAGGCCCT GCCCGAGAAC GCATCCCAGC AGATTCACGC CTTCTCCTCT
    ACCACCCTGG ATGACATCCT GCACGCCTTC TCTGAAGTCA GCGCTGCTCG CGTGGCGGGG
    GGCTACCTGC TCATGCTAGC CTATGCCTGT GTGACGATGC TGCGGTGGGA CTGTGCTCAG
    TCCCAGGGTG CAGTGGGCCT CGCTGGTGTG CTGCTGGTGG CCCTGGCAGT GGCCTCGGGC
    CTGGGGCTCT GTGCCCTGTT GGGCATCGCC TTCAACGCTG CCACTACCCA GGTGCTGCCT
    TTCTTGGCAC TGGGCATCGG CGTGGATGAC ATATTCCTGC TGGCACACGC CTTCACAGAG
    GCTCCACCTG GCACCCCTCT CCAGGAGCGC ACGGGGGAGT GTCTGCGGCG CACAGGCACC
    GGTGTCACAC TCACATCCGT CAACAATATG GTCGCCTTCT TCATGGCCGC CCTAGTCCCC
    ATCCCTGCAC TTCGGGCCTT CTCCTTGCAG GCAGCCATAG TGGTGGGCTG CAACTTTGCA
    GCCGTGATGC TTGTCTTCCC AGCGGTCCTC AGCCTGGACC TGCAGCGGCG CCACTGCCGG
    CGCCTGGATG TGCTCTGCTG CTTCTCTAGC CCCTGCTCTG CTCGGGTGAT TCAGATTCTG
    CCCCAGGAGC TGGGGAACGG GACGGTACCA GTGGGCGTTG CCCACCTGAC TGCCACGGTT
    CAAGCCTTTG CCCACTGTGA AGCCAGGAGC CAACATGTGG TCACCATCTT GCCTCCCCAA
    GCTCACCTGG TGCCGCCACC TTCTGACCCT TTGGGCTCTG AGCTCTTCAG CCCAGGAGGG
    TCCACACGGG ACCTTCTAGG GCAGGAGGAG GGGACAGGGC AGAAGGCAGC CTGCAAGTCC
    CTGCCCTGTG CCCGCTGGAA TCTTGCCCAT TTCGCCCGCT CTCAGTTTGC ACCCTTGTTG
    CTCCAGTCCC CCACCAAGCT TAGTCACCTT TCCCCACAGG CTGTAGTACT GGTACTTTTT
    GGGGCTCTCC TGGGCCTGAG CCTCTATGGA GCGACCTTGG TGCAGGACGG TCTGGCCCTG
    ACTGATGTGG TGCCGCGTGG CACCAAGGAG CATGCCTTCC TGAGCGCCCA GCTCAGGTAC
    TTCTCTCTGT ACGAGGTGGC TCTGGTGACA CAGGGTGGCT TTGACTACGC CCACTCCCAA
    CGCGCCCTCT TTGATCTGCA CCAGCGCTTC AGTTCCCTCA AGGCCGTGCT GCCCCCACCG
    GCCACCCAGG CACCCCGCAC CTGGCTGCAC TACTACCGCA GCTGGCTACA GGGAATCCAG
    GCTGCATTTG ACCAGGACTG GGCTTCTGGG CGCATCACCC GCCACTCATA CCGCAATGGC
    TCTGAGGATG GGGCCCTGGC ATACAAGCTG CTTGTCCAGA CCGGGGATGC CCAGGAGCCT
    CTAGATTTCA GCCAGCTGAC CACCAAGAAG CTGGTGGACA AGGAAGGGCT GATCCCACCC
    GAGCTCTTCT ACATGGGGCT GACCGTGTGG GTGAGCAGTG ACCCCCTGGG TCTGGCAGCC
    TCACAGGCCA ACTTCTATCC CCCACCTCCC GAGTGGCTGC ATGACAAGTA CGATACCACG
    GGGGAGAATC TTCGCATCCC GGCGGCCCAG CCCTTGGAGT TTGCCCAGTT CCCCTTCCTC
    CTGCGCGGCC TCCAGAAGAC TGCAGACTTC GTGGAGGCCA TCGAGGGGGC CCGGGCAGCG
    TGTGCCGAGG CCGGCCAGGC CGGGGTGCAT GCCTACCCCA GCGGCTCCCC CTTCCTCTTC
    TGGGAGCAGT ACCTGAGCCT GCGGCGCTGC TTCCTGCTGG CGGTCTGCAT CCTGCTGCTG
    TGTACTTTCC TCGTCTGTGC CCTGCTGCTG CTCAACCCCT GGACGGCTGT GCTCATAGTA
    CTGGTCCTGG CGATGATGAC TGTGGAGCTC TTTGGCATCA TGGGTTTCCT GGGCATCAAG
    CTGAGTGCCA TCCCGGTGGT GATTCTTGTG GCCTCCATAG GCATCGGTGT CGAGTTCACT
    GTCCACGTGG CTCTGGGCTT CCTGACCGCT CAGGGTAGCC GGAACCTGCG GGCTGCCCGG
    GCCCTGGAGC GCACATTTGC CCCAGTGACC GATGGGGCCA TCTCCACGTT GCTAGGTCTG
    CTCATGCTTG CTGGCTCCAA CTTTGACTTC ATCGTAAGGT ACTTCTTTGT GGTGCTGACA
    ATACTTACAC TCCTGGGCCT CCTCCACGGG CTCGTGCTGC TGCCGGTACT GCTGTCCATC
    CTGGGCCCCC CACCAGAGGT GGTACAGATG TACAAGGAGA GCACAGAGGT CCTGAGCCCC
    CCAGCTCCAC AGGGAGGAGG GCTTAGGTGG GGGGTACCCT CCACCCTGCC CCAGAGCTTT
    GCCAGAGTGA CTACCTCCAT GACTGTGGCC CTCCACCCAC CCCCACTGCC TGGTGCCTAC
    ATCCACCCAG CCTCTGAGGA GCCTACTTGG TCACCTGCTG CCACACCAGC TGCCAATGGC
    CCCAGCAACC TCGGGTCTAG GGGACTGTGT CCAGCCACGG GGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu10282
    Clone ID Related Accession (Same CDS sequence) XM_006052580.1
    Accession Version XM_006052580.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3624bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2014-03-11
    Organism Bubalus bubalis(water buffalo)
    Product protein patched homolog 2 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005784468.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCTCGGC CGCCACCTCT CGGGGAGCTG CCCCCGGGCT ACACACCCCC AGCTCGACCT 
    GCAACACCCC AGATCCTAGC TGGGAGCCTG AAGGCTCCGC TCTGGCTCCG TGCTTACTTC
    CAGGGCCTGC TCTTCTCTTT GGGCTGCGGG ATCCAGAGAC ACTGTGGCAA AGTGCTCTTC
    CTGGGACTGT TGGCCTTTGG GGCCCTAGCA CTGGGTCTCC GCGTGGCCAT CATTGAGACA
    GACCTAGAAC AGCTCTGGGT GGAAGTGGGC AGCCGGGTGA GCCAGGAACT GGAATACACC
    AAGGAGAAGC TGGGGGAGGA GGCTGCGTAT ACCTCCCAGA TGTTGATACA GACCCCGCGT
    CAGGAGGGGG AGAACGTCCT CACGCCCGAG GCACTTGACC TCCACCTCCA GGCAGCCCTC
    ACCGCCAGTA AAGTGCAAGT ATCCCTCTAT GGAAAGTCCT GGGATCTGAA CAAAATCTGC
    TACAAGTCAG GAGTTCCCCT AATTGAAAAT GGAATGATTG AGCGGATGAT TGAAAAGCTG
    TTTCCCTGCG TGATCCTCAC CCCCCTCGAC TGCTTCTGGG AGGGAGCCAA ACTCCAAGGG
    GGCTCTGCCT ACTTGCCGGG CCGCCCAGAC ATCCAGTGGA CCAACCTGGA CCCAGAGCAG
    CTGCTTGAGG AGCTGGGCCC CTTTGCCTCC CTTGAGGGCT TCCGGGAGCT GCTAGACAAG
    GCACAGGTGG GCCAGGCCTA CGTGGGTCGG CCCTGTCTGC ACCCTGACGA CCTCCACTGC
    CCACCTAGTG CCCCTAACCA TCACAGCAAG CAGGCTCCCA ACGTGGCTCA GGAGTTGAGT
    GGGGGCTGCC ATGGCTTCTC CCACAAGTTT ATGCACTGGC AGGAGGAGCT GCTGCTGGGA
    GGCATGGCCA GAGACCCCCA AGGACAGCTG CTGAGGGCAG AGGCCCTGCA GAGCACCTTC
    CTGCTAATGA GCCCCCGCCA GCTCTACGAG CACTTCCGAG GTGACTACCA GACGCATGAC
    ATCGGCTGGA GCGAGGAGCA GGCCGGCACG GTCCTTCAGG CCTGGCAGCG ACGCTTTGTG
    CAGCTGGCCC AGGAGGCCCT GCCCGAGAAC GCATCCCAGC AGATTCACGC CTTCTCCTCT
    ACCACCCTGG ATGACATCCT GCACGCCTTC TCTGAAGTCA GCGCTGCTCG CGTGGCGGGG
    GGCTACCTGC TCATGCTAGC CTATGCCTGT GTGACGATGC TGCGGTGGGA CTGTGCTCAG
    TCCCAGGGTG CAGTGGGCCT CGCTGGTGTG CTGCTGGTGG CCCTGGCAGT GGCCTCGGGC
    CTGGGGCTCT GTGCCCTGTT GGGCATCGCC TTCAACGCTG CCACTACCCA GGTGCTGCCT
    TTCTTGGCAC TGGGCATCGG CGTGGATGAC ATATTCCTGC TGGCACACGC CTTCACAGAG
    GCTCCACCTG GCACCCCTCT CCAGGAGCGC ACGGGGGAGT GTCTGCGGCG CACAGGCACC
    GGTGTCACAC TCACATCCGT CAACAATATG GTCGCCTTCT TCATGGCCGC CCTAGTCCCC
    ATCCCTGCAC TTCGGGCCTT CTCCTTGCAG GCAGCCATAG TGGTGGGCTG CAACTTTGCA
    GCCGTGATGC TTGTCTTCCC AGCGGTCCTC AGCCTGGACC TGCAGCGGCG CCACTGCCGG
    CGCCTGGATG TGCTCTGCTG CTTCTCTAGC CCCTGCTCTG CTCGGGTGAT TCAGATTCTG
    CCCCAGGAGC TGGGGAACGG GACGGTACCA GTGGGCGTTG CCCACCTGAC TGCCACGGTT
    CAAGCCTTTG CCCACTGTGA AGCCAGGAGC CAACATGTGG TCACCATCTT GCCTCCCCAA
    GCTCACCTGG TGCCGCCACC TTCTGACCCT TTGGGCTCTG AGCTCTTCAG CCCAGGAGGG
    TCCACACGGG ACCTTCTAGG GCAGGAGGAG GGGACAGGGC AGAAGGCAGC CTGCAAGTCC
    CTGCCCTGTG CCCGCTGGAA TCTTGCCCAT TTCGCCCGCT CTCAGTTTGC ACCCTTGTTG
    CTCCAGTCCC CCACCAAGGC TGTAGTACTG GTACTTTTTG GGGCTCTCCT GGGCCTGAGC
    CTCTATGGAG CGACCTTGGT GCAGGACGGT CTGGCCCTGA CTGATGTGGT GCCGCGTGGC
    ACCAAGGAGC ATGCCTTCCT GAGCGCCCAG CTCAGGTACT TCTCTCTGTA CGAGGTGGCT
    CTGGTGACAC AGGGTGGCTT TGACTACGCC CACTCCCAAC GCGCCCTCTT TGATCTGCAC
    CAGCGCTTCA GTTCCCTCAA GGCCGTGCTG CCCCCACCGG CCACCCAGGC ACCCCGCACC
    TGGCTGCACT ACTACCGCAG CTGGCTACAG GGAATCCAGG CTGCATTTGA CCAGGACTGG
    GCTTCTGGGC GCATCACCCG CCACTCATAC CGCAATGGCT CTGAGGATGG GGCCCTGGCA
    TACAAGCTGC TTGTCCAGAC CGGGGATGCC CAGGAGCCTC TAGATTTCAG CCAGCTGACC
    ACCAAGAAGC TGGTGGACAA GGAAGGGCTG ATCCCACCCG AGCTCTTCTA CATGGGGCTG
    ACCGTGTGGG TGAGCAGTGA CCCCCTGGGT CTGGCAGCCT CACAGGCCAA CTTCTATCCC
    CCACCTCCCG AGTGGCTGCA TGACAAGTAC GATACCACGG GGGAGAATCT TCGCATCCCG
    GCGGCCCAGC CCTTGGAGTT TGCCCAGTTC CCCTTCCTCC TGCGCGGCCT CCAGAAGACT
    GCAGACTTCG TGGAGGCCAT CGAGGGGGCC CGGGCAGCGT GTGCCGAGGC CGGCCAGGCC
    GGGGTGCATG CCTACCCCAG CGGCTCCCCC TTCCTCTTCT GGGAGCAGTA CCTGAGCCTG
    CGGCGCTGCT TCCTGCTGGC GGTCTGCATC CTGCTGCTGT GTACTTTCCT CGTCTGTGCC
    CTGCTGCTGC TCAACCCCTG GACGGCTGTG CTCATAGTAC TGGTCCTGGC GATGATGACT
    GTGGAGCTCT TTGGCATCAT GGGTTTCCTG GGCATCAAGC TGAGTGCCAT CCCGGTGGTG
    ATTCTTGTGG CCTCCATAGG CATCGGTGTC GAGTTCACTG TCCACGTGGC TCTGGGCTTC
    CTGACCGCTC AGGGTAGCCG GAACCTGCGG GCTGCCCGGG CCCTGGAGCG CACATTTGCC
    CCAGTGACCG ATGGGGCCAT CTCCACGTTG CTAGGTCTGC TCATGCTTGC TGGCTCCAAC
    TTTGACTTCA TCGTAAGGTA CTTCTTTGTG GTGCTGACAA TACTTACACT CCTGGGCCTC
    CTCCACGGGC TCGTGCTGCT GCCGGTACTG CTGTCCATCC TGGGCCCCCC ACCAGAGGTG
    GTACAGATGT ACAAGGAGAG CACAGAGGTC CTGAGCCCCC CAGCTCCACA GGGAGGAGGG
    CTTAGGTGGG GGGTACCCTC CACCCTGCCC CAGAGCTTTG CCAGAGTGAC TACCTCCATG
    ACTGTGGCCC TCCACCCACC CCCACTGCCT GGTGCCTACA TCCACCCAGC CTCTGAGGAG
    CCTACTTGGT CACCTGCTGC CACACCAGCT GCCAATGGCC CCAGCAACCT CGGGTCTAGG
    GGACTGTGTC CAGCCACGGG GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006052642.1
    CDS1796..5419
    Translation

    Target ORF information:

    RefSeq Version XM_006052580.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis patched 2 (PTCH2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006052580.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCTCGGC CGCCACCTCT CGGGGAGCTG CCCCCGGGCT ACACACCCCC AGCTCGACCT 
    GCAACACCCC AGATCCTAGC TGGGAGCCTG AAGGCTCCGC TCTGGCTCCG TGCTTACTTC
    CAGGGCCTGC TCTTCTCTTT GGGCTGCGGG ATCCAGAGAC ACTGTGGCAA AGTGCTCTTC
    CTGGGACTGT TGGCCTTTGG GGCCCTAGCA CTGGGTCTCC GCGTGGCCAT CATTGAGACA
    GACCTAGAAC AGCTCTGGGT GGAAGTGGGC AGCCGGGTGA GCCAGGAACT GGAATACACC
    AAGGAGAAGC TGGGGGAGGA GGCTGCGTAT ACCTCCCAGA TGTTGATACA GACCCCGCGT
    CAGGAGGGGG AGAACGTCCT CACGCCCGAG GCACTTGACC TCCACCTCCA GGCAGCCCTC
    ACCGCCAGTA AAGTGCAAGT ATCCCTCTAT GGAAAGTCCT GGGATCTGAA CAAAATCTGC
    TACAAGTCAG GAGTTCCCCT AATTGAAAAT GGAATGATTG AGCGGATGAT TGAAAAGCTG
    TTTCCCTGCG TGATCCTCAC CCCCCTCGAC TGCTTCTGGG AGGGAGCCAA ACTCCAAGGG
    GGCTCTGCCT ACTTGCCGGG CCGCCCAGAC ATCCAGTGGA CCAACCTGGA CCCAGAGCAG
    CTGCTTGAGG AGCTGGGCCC CTTTGCCTCC CTTGAGGGCT TCCGGGAGCT GCTAGACAAG
    GCACAGGTGG GCCAGGCCTA CGTGGGTCGG CCCTGTCTGC ACCCTGACGA CCTCCACTGC
    CCACCTAGTG CCCCTAACCA TCACAGCAAG CAGGCTCCCA ACGTGGCTCA GGAGTTGAGT
    GGGGGCTGCC ATGGCTTCTC CCACAAGTTT ATGCACTGGC AGGAGGAGCT GCTGCTGGGA
    GGCATGGCCA GAGACCCCCA AGGACAGCTG CTGAGGGCAG AGGCCCTGCA GAGCACCTTC
    CTGCTAATGA GCCCCCGCCA GCTCTACGAG CACTTCCGAG GTGACTACCA GACGCATGAC
    ATCGGCTGGA GCGAGGAGCA GGCCGGCACG GTCCTTCAGG CCTGGCAGCG ACGCTTTGTG
    CAGCTGGCCC AGGAGGCCCT GCCCGAGAAC GCATCCCAGC AGATTCACGC CTTCTCCTCT
    ACCACCCTGG ATGACATCCT GCACGCCTTC TCTGAAGTCA GCGCTGCTCG CGTGGCGGGG
    GGCTACCTGC TCATGCTAGC CTATGCCTGT GTGACGATGC TGCGGTGGGA CTGTGCTCAG
    TCCCAGGGTG CAGTGGGCCT CGCTGGTGTG CTGCTGGTGG CCCTGGCAGT GGCCTCGGGC
    CTGGGGCTCT GTGCCCTGTT GGGCATCGCC TTCAACGCTG CCACTACCCA GGTGCTGCCT
    TTCTTGGCAC TGGGCATCGG CGTGGATGAC ATATTCCTGC TGGCACACGC CTTCACAGAG
    GCTCCACCTG GCACCCCTCT CCAGGAGCGC ACGGGGGAGT GTCTGCGGCG CACAGGCACC
    GGTGTCACAC TCACATCCGT CAACAATATG GTCGCCTTCT TCATGGCCGC CCTAGTCCCC
    ATCCCTGCAC TTCGGGCCTT CTCCTTGCAG GCAGCCATAG TGGTGGGCTG CAACTTTGCA
    GCCGTGATGC TTGTCTTCCC AGCGGTCCTC AGCCTGGACC TGCAGCGGCG CCACTGCCGG
    CGCCTGGATG TGCTCTGCTG CTTCTCTAGC CCCTGCTCTG CTCGGGTGAT TCAGATTCTG
    CCCCAGGAGC TGGGGAACGG GACGGTACCA GTGGGCGTTG CCCACCTGAC TGCCACGGTT
    CAAGCCTTTG CCCACTGTGA AGCCAGGAGC CAACATGTGG TCACCATCTT GCCTCCCCAA
    GCTCACCTGG TGCCGCCACC TTCTGACCCT TTGGGCTCTG AGCTCTTCAG CCCAGGAGGG
    TCCACACGGG ACCTTCTAGG GCAGGAGGAG GGGACAGGGC AGAAGGCAGC CTGCAAGTCC
    CTGCCCTGTG CCCGCTGGAA TCTTGCCCAT TTCGCCCGCT CTCAGTTTGC ACCCTTGTTG
    CTCCAGTCCC CCACCAAGGC TGTAGTACTG GTACTTTTTG GGGCTCTCCT GGGCCTGAGC
    CTCTATGGAG CGACCTTGGT GCAGGACGGT CTGGCCCTGA CTGATGTGGT GCCGCGTGGC
    ACCAAGGAGC ATGCCTTCCT GAGCGCCCAG CTCAGGTACT TCTCTCTGTA CGAGGTGGCT
    CTGGTGACAC AGGGTGGCTT TGACTACGCC CACTCCCAAC GCGCCCTCTT TGATCTGCAC
    CAGCGCTTCA GTTCCCTCAA GGCCGTGCTG CCCCCACCGG CCACCCAGGC ACCCCGCACC
    TGGCTGCACT ACTACCGCAG CTGGCTACAG GGAATCCAGG CTGCATTTGA CCAGGACTGG
    GCTTCTGGGC GCATCACCCG CCACTCATAC CGCAATGGCT CTGAGGATGG GGCCCTGGCA
    TACAAGCTGC TTGTCCAGAC CGGGGATGCC CAGGAGCCTC TAGATTTCAG CCAGCTGACC
    ACCAAGAAGC TGGTGGACAA GGAAGGGCTG ATCCCACCCG AGCTCTTCTA CATGGGGCTG
    ACCGTGTGGG TGAGCAGTGA CCCCCTGGGT CTGGCAGCCT CACAGGCCAA CTTCTATCCC
    CCACCTCCCG AGTGGCTGCA TGACAAGTAC GATACCACGG GGGAGAATCT TCGCATCCCG
    GCGGCCCAGC CCTTGGAGTT TGCCCAGTTC CCCTTCCTCC TGCGCGGCCT CCAGAAGACT
    GCAGACTTCG TGGAGGCCAT CGAGGGGGCC CGGGCAGCGT GTGCCGAGGC CGGCCAGGCC
    GGGGTGCATG CCTACCCCAG CGGCTCCCCC TTCCTCTTCT GGGAGCAGTA CCTGAGCCTG
    CGGCGCTGCT TCCTGCTGGC GGTCTGCATC CTGCTGCTGT GTACTTTCCT CGTCTGTGCC
    CTGCTGCTGC TCAACCCCTG GACGGCTGTG CTCATAGTAC TGGTCCTGGC GATGATGACT
    GTGGAGCTCT TTGGCATCAT GGGTTTCCTG GGCATCAAGC TGAGTGCCAT CCCGGTGGTG
    ATTCTTGTGG CCTCCATAGG CATCGGTGTC GAGTTCACTG TCCACGTGGC TCTGGGCTTC
    CTGACCGCTC AGGGTAGCCG GAACCTGCGG GCTGCCCGGG CCCTGGAGCG CACATTTGCC
    CCAGTGACCG ATGGGGCCAT CTCCACGTTG CTAGGTCTGC TCATGCTTGC TGGCTCCAAC
    TTTGACTTCA TCGTAAGGTA CTTCTTTGTG GTGCTGACAA TACTTACACT CCTGGGCCTC
    CTCCACGGGC TCGTGCTGCT GCCGGTACTG CTGTCCATCC TGGGCCCCCC ACCAGAGGTG
    GTACAGATGT ACAAGGAGAG CACAGAGGTC CTGAGCCCCC CAGCTCCACA GGGAGGAGGG
    CTTAGGTGGG GGGTACCCTC CACCCTGCCC CAGAGCTTTG CCAGAGTGAC TACCTCCATG
    ACTGTGGCCC TCCACCCACC CCCACTGCCT GGTGCCTACA TCCACCCAGC CTCTGAGGAG
    CCTACTTGGT CACCTGCTGC CACACCAGCT GCCAATGGCC CCAGCAACCT CGGGTCTAGG
    GGACTGTGTC CAGCCACGGG GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu68862
    Clone ID Related Accession (Same CDS sequence) XM_006052576.2
    Accession Version XM_006052576.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3771bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product protein patched homolog 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037550.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006052576.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCTCGGC CGCCACCTCT CGGGGAGCTG CCCCCGGGCT ACACACCCCC AGCTCGACCT 
    GCAACACCCC AGATCCTAGC TGGGAGCCTG AAGGCTCCGC TCTGGCTCCG TGCTTACTTC
    CAGGGCCTGC TCTTCTCTTT GGGCTGCGGG ATCCAGAGAC ACTGTGGCAA AGTGCTCTTC
    CTGGGACTGT TGGCCTTTGG GGCCCTAGCA CTGGGTCTCC GCGTGGCCAT CATTGAGACA
    GACCTAGAAC AGCTCTGGGT GGAAGTGGGC AGCCGGGTGA GCCAGGAACT GGAATACACC
    AAGGAGAAGC TGGGGGAGGA GGCTGCGTAT ACCTCCCAGA TGTTGATACA GACCCCGCGT
    CAGGAGGGGG AGAACGTCCT CACGCCCGAG GCACTTGACC TCCACCTCCA GGCAGCCCTC
    ACCGCCAGTA AAGTGCAAGT ATCCCTCTAT GGAAAGTCCT GGGATCTGAA CAAAATCTGC
    TACAAGTCAG GAGTTCCCCT AATTGAAAAT GGAATGATTG AGCGGATGAT TGAAAAGCTG
    TTTCCCTGCG TGATCCTCAC CCCCCTCGAC TGCTTCTGGG AGGGAGCCAA ACTCCAAGGG
    GGCTCTGCCT ACTTGCCGGG CCGCCCAGAC ATCCAGTGGA CCAACCTGGA CCCAGAGCAG
    CTGCTTGAGG AGCTGGGCCC CTTTGCCTCC CTTGAGGGCT TCCGGGAGCT GCTAGACAAG
    GCACAGGTGG GCCAGGCCTA CGTGGGTCGG CCCTGTCTGC ACCCTGACGA CCTCCACTGC
    CCACCTAGTG CCCCTAACCA TCACAGCAAG CAGGCTCCCA ACGTGGCTCA GGAGTTGAGT
    GGGGGCTGCC ATGGCTTCTC CCACAAGTTT ATGCACTGGC AGGAGGAGCT GCTGCTGGGA
    GGCATGGCCA GAGACCCCCA AGGACAGCTG CTGAGGGCAG AGGCCCTGCA GAGCACCTTC
    CTGCTAATGA GCCCCCGCCA GCTCTACGAG CACTTCCGAG GTGACTACCA GACGCATGAC
    ATCGGCTGGA GCGAGGAGCA GGCCGGCACG GTCCTTCAGG CCTGGCAGCG ACGCTTTGTG
    CAGCTGGCCC AGGAGGCCCT GCCCGAGAAC GCATCCCAGC AGATTCACGC CTTCTCCTCT
    ACCACCCTGG ATGACATCCT GCACGCCTTC TCTGAAGTCA GCGCTGCTCG CGTGGCGGGG
    GGCTACCTGC TCATGCTAGC CTATGCCTGT GTGACGATGC TGCGGTGGGA CTGTGCTCAG
    TCCCAGGGTG CAGTGGGCCT CGCTGGTGTG CTGCTGGTGG CCCTGGCAGT GGCCTCGGGC
    CTGGGGCTCT GTGCCCTGTT GGGCATCGCC TTCAACGCTG CCACTACCCA GGTGCTGCCT
    TTCTTGGCAC TGGGCATCGG CGTGGATGAC ATATTCCTGC TGGCACACGC CTTCACAGAG
    GCTCCACCTG GCACCCCTCT CCAGGAGCGC ACGGGGGAGT GTCTGCGGCG CACAGGCACC
    GGTGTCACAC TCACATCCGT CAACAATATG GTCGCCTTCT TCATGGCCGC CCTAGTCCCC
    ATCCCTGCAC TTCGGGCCTT CTCCTTGCAG GCAGCCATAG TGGTGGGCTG CAACTTTGCA
    GCCGTGATGC TTGTCTTCCC AGCGGTCCTC AGCCTGGACC TGCAGCGGCG CCACTGCCGG
    CGCCTGGATG TGCTCTGCTG CTTCTCTAGC CCCTGCTCTG CTCAGGTGAT TCAGATTCTG
    CCCCAGGAGC TGGGGAACGG GACGGTACCA GTGGGCGTTG CCCACCTGAC TGCCACGGTT
    CAAGCCTTTG CCCACTGTGA AGCCAGGAGC CAACATGTGG TCACCATCTT GCCTCCCCAA
    GCTCACCTGG TGCCGCCACC TTCTGACCCT TTGGGCTCTG AGCTCTTCAG CCCAGGAGGG
    TCCACACGGG ACCTTCTAGG GCAGGAGGAG GGGACAGGGC AGAAGGCAGC CTGCAAGTCC
    CTGCCCTGTG CCCGCTGGAA TCTTGCCCAT TTCGCCCGCT CTCAGTTTGC ACCCTTGTTG
    CTCCAGTCCC CCACCAAGCT TAGTCACCTT TCCCCACAGG CTGTAGTACT GGTACTTTTT
    GGGGCTCTCC TGGGCCTGAG CCTCTATGGA GCGACCTTGG TGCAGGACGG TCTGGCCCTG
    ACTGATGTGG TGCCGCGTGG CACCAAGGAG CATGCCTTCC TGAGCGCCCA GCTCAGGTAC
    TTCTCTCTGT ACGAGGTGGC TCTGGTGACA CAGGGTGGCT TTGACTACGC CCACTCCCAA
    CGCGCCCTCT TTGATCTGCA CCAGCGCTTC AGTTCCCTCA AGGCCGTGCT GCCCCCACCG
    GCCACCCAGG CACCCCGCAC CTGGCTGCAC TACTACCGCA GCTGGCTACA GGGAATCCAG
    GCTGCGTTTG ACCAGGACTG GGCTTCTGGG CGCATCACCC GCCACTCATA CCGCAATGGC
    TCTGAGGATG GGGCCCTGGC ATACAAGCTG CTTGTCCAGA CCGGGGATGC CCAGGAGCCT
    CTAGATTTCA GCCAGCTGAC CACCAAGAAG CTGGTGGACA AGGAAGGGCT GATCCCACCC
    GAGCTCTTCT ACATGGGGCT GACCGTGTGG GTGAGCAGTG ACCCCCTGGG TCTGGCAGCC
    TCACAGGCCA ACTTCTATCC CCCACCTCCT GAGTGGCTGC ATGACAAGTA CGATACCACG
    GGGGAGAATC TTCGCAGTGA GTTCTGGGGG AGCCCGAACT GCCTGGGCCC AAGCCCACCC
    AGGCCCTCCC CTGAAGCCCT GCCCACTGCT GCCAGAGCTC ACCGGCCGCC CCTCCCTCTC
    CCTCCGACCC CTCCCTCCAC AGTCCCGGCG GCCCAGCCCT TGGAGTTTGC CCAGTTCCCC
    TTCCTCCTGC GCGGCCTCCA GAAGACTGCA GACTTCGTGG AGGCCATCGA GGGGGCCCGG
    GCAGCGTGTG CCGAGGCCGG CCAGGCCGGG GTGCATGCCT ACCCCAGCGG CTCCCCCTTC
    CTCTTCTGGG AGCAGTACCT GAGCCTGCGG CGCTGCTTCC TGCTAGCGGT CTGCATCCTG
    CTGCTGTGTA CTTTCCTCGT CTGTGCCCTG CTGCTGCTCA ACCCCTGGAC GGCTGTGCTC
    ATAGTACTGG TCCTGGCGAT GATGACTGTG GAGCTCTTTG GCATCATGGG TTTCCTGGGC
    ATCAAGCTGA GTGCCATCCC GGTGGTGATT CTTGTGGCCT CCATAGGCAT CGGTGTCGAG
    TTCACTGTCC ACGTGGCTCT GGGCTTCCTG ACCGCTCAGG GTAGCCGGAA CCTGCGGGCT
    GCCCGGGCCC TGGAGCGCAC ATTTGCCCCA GTGACCGATG GGGCCATCTC CACGTTGCTA
    GGTCTGCTCA TGCTTGCTGG CTCCAACTTT GACTTCATCG TAAGGTACTT CTTTGTGGTG
    CTGACAATAC TTACACTCCT GGGCCTCCTC CACGGGCTCG TGCTGCTGCC GGTACTGCTG
    TCCATCCTGG GCCCCCCACC AGAGGTGGTA CAGATGTACA AGGAGAGCAC AGAGGTCCTG
    AGCCCCCCAG CTCCACAGGG AGGAGGGCTT AGGTGGGGGG TACCCTCCAC CCTGCCCCAG
    AGCTTTGCCA GAGTGACTAC CTCCATGACT GTGGCCCTCC ACCCACCCCC ACTGCCTGGT
    GCCTACATCC ACCCAGCCTC TGAGGAGCCT ACTTGGTCAC CTGCTGCCAC ACCAGCTGCC
    AATGGCCCCA GCAACCTCGG GTCTAGGGGA CTGTGTCCAG CCACGGGGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006052638.2
    CDS433..4203
    Translation

    Target ORF information:

    RefSeq Version XM_006052576.2
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis patched 2 (PTCH2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006052576.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCTCGGC CGCCACCTCT CGGGGAGCTG CCCCCGGGCT ACACACCCCC AGCTCGACCT 
    GCAACACCCC AGATCCTAGC TGGGAGCCTG AAGGCTCCGC TCTGGCTCCG TGCTTACTTC
    CAGGGCCTGC TCTTCTCTTT GGGCTGCGGG ATCCAGAGAC ACTGTGGCAA AGTGCTCTTC
    CTGGGACTGT TGGCCTTTGG GGCCCTAGCA CTGGGTCTCC GCGTGGCCAT CATTGAGACA
    GACCTAGAAC AGCTCTGGGT GGAAGTGGGC AGCCGGGTGA GCCAGGAACT GGAATACACC
    AAGGAGAAGC TGGGGGAGGA GGCTGCGTAT ACCTCCCAGA TGTTGATACA GACCCCGCGT
    CAGGAGGGGG AGAACGTCCT CACGCCCGAG GCACTTGACC TCCACCTCCA GGCAGCCCTC
    ACCGCCAGTA AAGTGCAAGT ATCCCTCTAT GGAAAGTCCT GGGATCTGAA CAAAATCTGC
    TACAAGTCAG GAGTTCCCCT AATTGAAAAT GGAATGATTG AGCGGATGAT TGAAAAGCTG
    TTTCCCTGCG TGATCCTCAC CCCCCTCGAC TGCTTCTGGG AGGGAGCCAA ACTCCAAGGG
    GGCTCTGCCT ACTTGCCGGG CCGCCCAGAC ATCCAGTGGA CCAACCTGGA CCCAGAGCAG
    CTGCTTGAGG AGCTGGGCCC CTTTGCCTCC CTTGAGGGCT TCCGGGAGCT GCTAGACAAG
    GCACAGGTGG GCCAGGCCTA CGTGGGTCGG CCCTGTCTGC ACCCTGACGA CCTCCACTGC
    CCACCTAGTG CCCCTAACCA TCACAGCAAG CAGGCTCCCA ACGTGGCTCA GGAGTTGAGT
    GGGGGCTGCC ATGGCTTCTC CCACAAGTTT ATGCACTGGC AGGAGGAGCT GCTGCTGGGA
    GGCATGGCCA GAGACCCCCA AGGACAGCTG CTGAGGGCAG AGGCCCTGCA GAGCACCTTC
    CTGCTAATGA GCCCCCGCCA GCTCTACGAG CACTTCCGAG GTGACTACCA GACGCATGAC
    ATCGGCTGGA GCGAGGAGCA GGCCGGCACG GTCCTTCAGG CCTGGCAGCG ACGCTTTGTG
    CAGCTGGCCC AGGAGGCCCT GCCCGAGAAC GCATCCCAGC AGATTCACGC CTTCTCCTCT
    ACCACCCTGG ATGACATCCT GCACGCCTTC TCTGAAGTCA GCGCTGCTCG CGTGGCGGGG
    GGCTACCTGC TCATGCTAGC CTATGCCTGT GTGACGATGC TGCGGTGGGA CTGTGCTCAG
    TCCCAGGGTG CAGTGGGCCT CGCTGGTGTG CTGCTGGTGG CCCTGGCAGT GGCCTCGGGC
    CTGGGGCTCT GTGCCCTGTT GGGCATCGCC TTCAACGCTG CCACTACCCA GGTGCTGCCT
    TTCTTGGCAC TGGGCATCGG CGTGGATGAC ATATTCCTGC TGGCACACGC CTTCACAGAG
    GCTCCACCTG GCACCCCTCT CCAGGAGCGC ACGGGGGAGT GTCTGCGGCG CACAGGCACC
    GGTGTCACAC TCACATCCGT CAACAATATG GTCGCCTTCT TCATGGCCGC CCTAGTCCCC
    ATCCCTGCAC TTCGGGCCTT CTCCTTGCAG GCAGCCATAG TGGTGGGCTG CAACTTTGCA
    GCCGTGATGC TTGTCTTCCC AGCGGTCCTC AGCCTGGACC TGCAGCGGCG CCACTGCCGG
    CGCCTGGATG TGCTCTGCTG CTTCTCTAGC CCCTGCTCTG CTCAGGTGAT TCAGATTCTG
    CCCCAGGAGC TGGGGAACGG GACGGTACCA GTGGGCGTTG CCCACCTGAC TGCCACGGTT
    CAAGCCTTTG CCCACTGTGA AGCCAGGAGC CAACATGTGG TCACCATCTT GCCTCCCCAA
    GCTCACCTGG TGCCGCCACC TTCTGACCCT TTGGGCTCTG AGCTCTTCAG CCCAGGAGGG
    TCCACACGGG ACCTTCTAGG GCAGGAGGAG GGGACAGGGC AGAAGGCAGC CTGCAAGTCC
    CTGCCCTGTG CCCGCTGGAA TCTTGCCCAT TTCGCCCGCT CTCAGTTTGC ACCCTTGTTG
    CTCCAGTCCC CCACCAAGCT TAGTCACCTT TCCCCACAGG CTGTAGTACT GGTACTTTTT
    GGGGCTCTCC TGGGCCTGAG CCTCTATGGA GCGACCTTGG TGCAGGACGG TCTGGCCCTG
    ACTGATGTGG TGCCGCGTGG CACCAAGGAG CATGCCTTCC TGAGCGCCCA GCTCAGGTAC
    TTCTCTCTGT ACGAGGTGGC TCTGGTGACA CAGGGTGGCT TTGACTACGC CCACTCCCAA
    CGCGCCCTCT TTGATCTGCA CCAGCGCTTC AGTTCCCTCA AGGCCGTGCT GCCCCCACCG
    GCCACCCAGG CACCCCGCAC CTGGCTGCAC TACTACCGCA GCTGGCTACA GGGAATCCAG
    GCTGCGTTTG ACCAGGACTG GGCTTCTGGG CGCATCACCC GCCACTCATA CCGCAATGGC
    TCTGAGGATG GGGCCCTGGC ATACAAGCTG CTTGTCCAGA CCGGGGATGC CCAGGAGCCT
    CTAGATTTCA GCCAGCTGAC CACCAAGAAG CTGGTGGACA AGGAAGGGCT GATCCCACCC
    GAGCTCTTCT ACATGGGGCT GACCGTGTGG GTGAGCAGTG ACCCCCTGGG TCTGGCAGCC
    TCACAGGCCA ACTTCTATCC CCCACCTCCT GAGTGGCTGC ATGACAAGTA CGATACCACG
    GGGGAGAATC TTCGCAGTGA GTTCTGGGGG AGCCCGAACT GCCTGGGCCC AAGCCCACCC
    AGGCCCTCCC CTGAAGCCCT GCCCACTGCT GCCAGAGCTC ACCGGCCGCC CCTCCCTCTC
    CCTCCGACCC CTCCCTCCAC AGTCCCGGCG GCCCAGCCCT TGGAGTTTGC CCAGTTCCCC
    TTCCTCCTGC GCGGCCTCCA GAAGACTGCA GACTTCGTGG AGGCCATCGA GGGGGCCCGG
    GCAGCGTGTG CCGAGGCCGG CCAGGCCGGG GTGCATGCCT ACCCCAGCGG CTCCCCCTTC
    CTCTTCTGGG AGCAGTACCT GAGCCTGCGG CGCTGCTTCC TGCTAGCGGT CTGCATCCTG
    CTGCTGTGTA CTTTCCTCGT CTGTGCCCTG CTGCTGCTCA ACCCCTGGAC GGCTGTGCTC
    ATAGTACTGG TCCTGGCGAT GATGACTGTG GAGCTCTTTG GCATCATGGG TTTCCTGGGC
    ATCAAGCTGA GTGCCATCCC GGTGGTGATT CTTGTGGCCT CCATAGGCAT CGGTGTCGAG
    TTCACTGTCC ACGTGGCTCT GGGCTTCCTG ACCGCTCAGG GTAGCCGGAA CCTGCGGGCT
    GCCCGGGCCC TGGAGCGCAC ATTTGCCCCA GTGACCGATG GGGCCATCTC CACGTTGCTA
    GGTCTGCTCA TGCTTGCTGG CTCCAACTTT GACTTCATCG TAAGGTACTT CTTTGTGGTG
    CTGACAATAC TTACACTCCT GGGCCTCCTC CACGGGCTCG TGCTGCTGCC GGTACTGCTG
    TCCATCCTGG GCCCCCCACC AGAGGTGGTA CAGATGTACA AGGAGAGCAC AGAGGTCCTG
    AGCCCCCCAG CTCCACAGGG AGGAGGGCTT AGGTGGGGGG TACCCTCCAC CCTGCCCCAG
    AGCTTTGCCA GAGTGACTAC CTCCATGACT GTGGCCCTCC ACCCACCCCC ACTGCCTGGT
    GCCTACATCC ACCCAGCCTC TGAGGAGCCT ACTTGGTCAC CTGCTGCCAC ACCAGCTGCC
    AATGGCCCCA GCAACCTCGG GTCTAGGGGA CTGTGTCCAG CCACGGGGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu68863
    Clone ID Related Accession (Same CDS sequence) XM_006052577.2
    Accession Version XM_006052577.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3750bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product protein patched homolog 2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037550.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006052577.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCTCGGC CGCCACCTCT CGGGGAGCTG CCCCCGGGCT ACACACCCCC AGCTCGACCT 
    GCAACACCCC AGATCCTAGC TGGGAGCCTG AAGGCTCCGC TCTGGCTCCG TGCTTACTTC
    CAGGGCCTGC TCTTCTCTTT GGGCTGCGGG ATCCAGAGAC ACTGTGGCAA AGTGCTCTTC
    CTGGGACTGT TGGCCTTTGG GGCCCTAGCA CTGGGTCTCC GCGTGGCCAT CATTGAGACA
    GACCTAGAAC AGCTCTGGGT GGAAGTGGGC AGCCGGGTGA GCCAGGAACT GGAATACACC
    AAGGAGAAGC TGGGGGAGGA GGCTGCGTAT ACCTCCCAGA TGTTGATACA GACCCCGCGT
    CAGGAGGGGG AGAACGTCCT CACGCCCGAG GCACTTGACC TCCACCTCCA GGCAGCCCTC
    ACCGCCAGTA AAGTGCAAGT ATCCCTCTAT GGAAAGTCCT GGGATCTGAA CAAAATCTGC
    TACAAGTCAG GAGTTCCCCT AATTGAAAAT GGAATGATTG AGCGGATGAT TGAAAAGCTG
    TTTCCCTGCG TGATCCTCAC CCCCCTCGAC TGCTTCTGGG AGGGAGCCAA ACTCCAAGGG
    GGCTCTGCCT ACTTGCCGGG CCGCCCAGAC ATCCAGTGGA CCAACCTGGA CCCAGAGCAG
    CTGCTTGAGG AGCTGGGCCC CTTTGCCTCC CTTGAGGGCT TCCGGGAGCT GCTAGACAAG
    GCACAGGTGG GCCAGGCCTA CGTGGGTCGG CCCTGTCTGC ACCCTGACGA CCTCCACTGC
    CCACCTAGTG CCCCTAACCA TCACAGCAAG CAGGCTCCCA ACGTGGCTCA GGAGTTGAGT
    GGGGGCTGCC ATGGCTTCTC CCACAAGTTT ATGCACTGGC AGGAGGAGCT GCTGCTGGGA
    GGCATGGCCA GAGACCCCCA AGGACAGCTG CTGAGGGCAG AGGCCCTGCA GAGCACCTTC
    CTGCTAATGA GCCCCCGCCA GCTCTACGAG CACTTCCGAG GTGACTACCA GACGCATGAC
    ATCGGCTGGA GCGAGGAGCA GGCCGGCACG GTCCTTCAGG CCTGGCAGCG ACGCTTTGTG
    CAGCTGGCCC AGGAGGCCCT GCCCGAGAAC GCATCCCAGC AGATTCACGC CTTCTCCTCT
    ACCACCCTGG ATGACATCCT GCACGCCTTC TCTGAAGTCA GCGCTGCTCG CGTGGCGGGG
    GGCTACCTGC TCATGCTAGC CTATGCCTGT GTGACGATGC TGCGGTGGGA CTGTGCTCAG
    TCCCAGGGTG CAGTGGGCCT CGCTGGTGTG CTGCTGGTGG CCCTGGCAGT GGCCTCGGGC
    CTGGGGCTCT GTGCCCTGTT GGGCATCGCC TTCAACGCTG CCACTACCCA GGTGCTGCCT
    TTCTTGGCAC TGGGCATCGG CGTGGATGAC ATATTCCTGC TGGCACACGC CTTCACAGAG
    GCTCCACCTG GCACCCCTCT CCAGGAGCGC ACGGGGGAGT GTCTGCGGCG CACAGGCACC
    GGTGTCACAC TCACATCCGT CAACAATATG GTCGCCTTCT TCATGGCCGC CCTAGTCCCC
    ATCCCTGCAC TTCGGGCCTT CTCCTTGCAG GCAGCCATAG TGGTGGGCTG CAACTTTGCA
    GCCGTGATGC TTGTCTTCCC AGCGGTCCTC AGCCTGGACC TGCAGCGGCG CCACTGCCGG
    CGCCTGGATG TGCTCTGCTG CTTCTCTAGC CCCTGCTCTG CTCAGGTGAT TCAGATTCTG
    CCCCAGGAGC TGGGGAACGG GACGGTACCA GTGGGCGTTG CCCACCTGAC TGCCACGGTT
    CAAGCCTTTG CCCACTGTGA AGCCAGGAGC CAACATGTGG TCACCATCTT GCCTCCCCAA
    GCTCACCTGG TGCCGCCACC TTCTGACCCT TTGGGCTCTG AGCTCTTCAG CCCAGGAGGG
    TCCACACGGG ACCTTCTAGG GCAGGAGGAG GGGACAGGGC AGAAGGCAGC CTGCAAGTCC
    CTGCCCTGTG CCCGCTGGAA TCTTGCCCAT TTCGCCCGCT CTCAGTTTGC ACCCTTGTTG
    CTCCAGTCCC CCACCAAGGC TGTAGTACTG GTACTTTTTG GGGCTCTCCT GGGCCTGAGC
    CTCTATGGAG CGACCTTGGT GCAGGACGGT CTGGCCCTGA CTGATGTGGT GCCGCGTGGC
    ACCAAGGAGC ATGCCTTCCT GAGCGCCCAG CTCAGGTACT TCTCTCTGTA CGAGGTGGCT
    CTGGTGACAC AGGGTGGCTT TGACTACGCC CACTCCCAAC GCGCCCTCTT TGATCTGCAC
    CAGCGCTTCA GTTCCCTCAA GGCCGTGCTG CCCCCACCGG CCACCCAGGC ACCCCGCACC
    TGGCTGCACT ACTACCGCAG CTGGCTACAG GGAATCCAGG CTGCGTTTGA CCAGGACTGG
    GCTTCTGGGC GCATCACCCG CCACTCATAC CGCAATGGCT CTGAGGATGG GGCCCTGGCA
    TACAAGCTGC TTGTCCAGAC CGGGGATGCC CAGGAGCCTC TAGATTTCAG CCAGCTGACC
    ACCAAGAAGC TGGTGGACAA GGAAGGGCTG ATCCCACCCG AGCTCTTCTA CATGGGGCTG
    ACCGTGTGGG TGAGCAGTGA CCCCCTGGGT CTGGCAGCCT CACAGGCCAA CTTCTATCCC
    CCACCTCCTG AGTGGCTGCA TGACAAGTAC GATACCACGG GGGAGAATCT TCGCAGTGAG
    TTCTGGGGGA GCCCGAACTG CCTGGGCCCA AGCCCACCCA GGCCCTCCCC TGAAGCCCTG
    CCCACTGCTG CCAGAGCTCA CCGGCCGCCC CTCCCTCTCC CTCCGACCCC TCCCTCCACA
    GTCCCGGCGG CCCAGCCCTT GGAGTTTGCC CAGTTCCCCT TCCTCCTGCG CGGCCTCCAG
    AAGACTGCAG ACTTCGTGGA GGCCATCGAG GGGGCCCGGG CAGCGTGTGC CGAGGCCGGC
    CAGGCCGGGG TGCATGCCTA CCCCAGCGGC TCCCCCTTCC TCTTCTGGGA GCAGTACCTG
    AGCCTGCGGC GCTGCTTCCT GCTAGCGGTC TGCATCCTGC TGCTGTGTAC TTTCCTCGTC
    TGTGCCCTGC TGCTGCTCAA CCCCTGGACG GCTGTGCTCA TAGTACTGGT CCTGGCGATG
    ATGACTGTGG AGCTCTTTGG CATCATGGGT TTCCTGGGCA TCAAGCTGAG TGCCATCCCG
    GTGGTGATTC TTGTGGCCTC CATAGGCATC GGTGTCGAGT TCACTGTCCA CGTGGCTCTG
    GGCTTCCTGA CCGCTCAGGG TAGCCGGAAC CTGCGGGCTG CCCGGGCCCT GGAGCGCACA
    TTTGCCCCAG TGACCGATGG GGCCATCTCC ACGTTGCTAG GTCTGCTCAT GCTTGCTGGC
    TCCAACTTTG ACTTCATCGT AAGGTACTTC TTTGTGGTGC TGACAATACT TACACTCCTG
    GGCCTCCTCC ACGGGCTCGT GCTGCTGCCG GTACTGCTGT CCATCCTGGG CCCCCCACCA
    GAGGTGGTAC AGATGTACAA GGAGAGCACA GAGGTCCTGA GCCCCCCAGC TCCACAGGGA
    GGAGGGCTTA GGTGGGGGGT ACCCTCCACC CTGCCCCAGA GCTTTGCCAG AGTGACTACC
    TCCATGACTG TGGCCCTCCA CCCACCCCCA CTGCCTGGTG CCTACATCCA CCCAGCCTCT
    GAGGAGCCTA CTTGGTCACC TGCTGCCACA CCAGCTGCCA ATGGCCCCAG CAACCTCGGG
    TCTAGGGGAC TGTGTCCAGC CACGGGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006052639.2
    CDS430..4179
    Translation

    Target ORF information:

    RefSeq Version XM_006052577.2
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis patched 2 (PTCH2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006052577.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCTCGGC CGCCACCTCT CGGGGAGCTG CCCCCGGGCT ACACACCCCC AGCTCGACCT 
    GCAACACCCC AGATCCTAGC TGGGAGCCTG AAGGCTCCGC TCTGGCTCCG TGCTTACTTC
    CAGGGCCTGC TCTTCTCTTT GGGCTGCGGG ATCCAGAGAC ACTGTGGCAA AGTGCTCTTC
    CTGGGACTGT TGGCCTTTGG GGCCCTAGCA CTGGGTCTCC GCGTGGCCAT CATTGAGACA
    GACCTAGAAC AGCTCTGGGT GGAAGTGGGC AGCCGGGTGA GCCAGGAACT GGAATACACC
    AAGGAGAAGC TGGGGGAGGA GGCTGCGTAT ACCTCCCAGA TGTTGATACA GACCCCGCGT
    CAGGAGGGGG AGAACGTCCT CACGCCCGAG GCACTTGACC TCCACCTCCA GGCAGCCCTC
    ACCGCCAGTA AAGTGCAAGT ATCCCTCTAT GGAAAGTCCT GGGATCTGAA CAAAATCTGC
    TACAAGTCAG GAGTTCCCCT AATTGAAAAT GGAATGATTG AGCGGATGAT TGAAAAGCTG
    TTTCCCTGCG TGATCCTCAC CCCCCTCGAC TGCTTCTGGG AGGGAGCCAA ACTCCAAGGG
    GGCTCTGCCT ACTTGCCGGG CCGCCCAGAC ATCCAGTGGA CCAACCTGGA CCCAGAGCAG
    CTGCTTGAGG AGCTGGGCCC CTTTGCCTCC CTTGAGGGCT TCCGGGAGCT GCTAGACAAG
    GCACAGGTGG GCCAGGCCTA CGTGGGTCGG CCCTGTCTGC ACCCTGACGA CCTCCACTGC
    CCACCTAGTG CCCCTAACCA TCACAGCAAG CAGGCTCCCA ACGTGGCTCA GGAGTTGAGT
    GGGGGCTGCC ATGGCTTCTC CCACAAGTTT ATGCACTGGC AGGAGGAGCT GCTGCTGGGA
    GGCATGGCCA GAGACCCCCA AGGACAGCTG CTGAGGGCAG AGGCCCTGCA GAGCACCTTC
    CTGCTAATGA GCCCCCGCCA GCTCTACGAG CACTTCCGAG GTGACTACCA GACGCATGAC
    ATCGGCTGGA GCGAGGAGCA GGCCGGCACG GTCCTTCAGG CCTGGCAGCG ACGCTTTGTG
    CAGCTGGCCC AGGAGGCCCT GCCCGAGAAC GCATCCCAGC AGATTCACGC CTTCTCCTCT
    ACCACCCTGG ATGACATCCT GCACGCCTTC TCTGAAGTCA GCGCTGCTCG CGTGGCGGGG
    GGCTACCTGC TCATGCTAGC CTATGCCTGT GTGACGATGC TGCGGTGGGA CTGTGCTCAG
    TCCCAGGGTG CAGTGGGCCT CGCTGGTGTG CTGCTGGTGG CCCTGGCAGT GGCCTCGGGC
    CTGGGGCTCT GTGCCCTGTT GGGCATCGCC TTCAACGCTG CCACTACCCA GGTGCTGCCT
    TTCTTGGCAC TGGGCATCGG CGTGGATGAC ATATTCCTGC TGGCACACGC CTTCACAGAG
    GCTCCACCTG GCACCCCTCT CCAGGAGCGC ACGGGGGAGT GTCTGCGGCG CACAGGCACC
    GGTGTCACAC TCACATCCGT CAACAATATG GTCGCCTTCT TCATGGCCGC CCTAGTCCCC
    ATCCCTGCAC TTCGGGCCTT CTCCTTGCAG GCAGCCATAG TGGTGGGCTG CAACTTTGCA
    GCCGTGATGC TTGTCTTCCC AGCGGTCCTC AGCCTGGACC TGCAGCGGCG CCACTGCCGG
    CGCCTGGATG TGCTCTGCTG CTTCTCTAGC CCCTGCTCTG CTCAGGTGAT TCAGATTCTG
    CCCCAGGAGC TGGGGAACGG GACGGTACCA GTGGGCGTTG CCCACCTGAC TGCCACGGTT
    CAAGCCTTTG CCCACTGTGA AGCCAGGAGC CAACATGTGG TCACCATCTT GCCTCCCCAA
    GCTCACCTGG TGCCGCCACC TTCTGACCCT TTGGGCTCTG AGCTCTTCAG CCCAGGAGGG
    TCCACACGGG ACCTTCTAGG GCAGGAGGAG GGGACAGGGC AGAAGGCAGC CTGCAAGTCC
    CTGCCCTGTG CCCGCTGGAA TCTTGCCCAT TTCGCCCGCT CTCAGTTTGC ACCCTTGTTG
    CTCCAGTCCC CCACCAAGGC TGTAGTACTG GTACTTTTTG GGGCTCTCCT GGGCCTGAGC
    CTCTATGGAG CGACCTTGGT GCAGGACGGT CTGGCCCTGA CTGATGTGGT GCCGCGTGGC
    ACCAAGGAGC ATGCCTTCCT GAGCGCCCAG CTCAGGTACT TCTCTCTGTA CGAGGTGGCT
    CTGGTGACAC AGGGTGGCTT TGACTACGCC CACTCCCAAC GCGCCCTCTT TGATCTGCAC
    CAGCGCTTCA GTTCCCTCAA GGCCGTGCTG CCCCCACCGG CCACCCAGGC ACCCCGCACC
    TGGCTGCACT ACTACCGCAG CTGGCTACAG GGAATCCAGG CTGCGTTTGA CCAGGACTGG
    GCTTCTGGGC GCATCACCCG CCACTCATAC CGCAATGGCT CTGAGGATGG GGCCCTGGCA
    TACAAGCTGC TTGTCCAGAC CGGGGATGCC CAGGAGCCTC TAGATTTCAG CCAGCTGACC
    ACCAAGAAGC TGGTGGACAA GGAAGGGCTG ATCCCACCCG AGCTCTTCTA CATGGGGCTG
    ACCGTGTGGG TGAGCAGTGA CCCCCTGGGT CTGGCAGCCT CACAGGCCAA CTTCTATCCC
    CCACCTCCTG AGTGGCTGCA TGACAAGTAC GATACCACGG GGGAGAATCT TCGCAGTGAG
    TTCTGGGGGA GCCCGAACTG CCTGGGCCCA AGCCCACCCA GGCCCTCCCC TGAAGCCCTG
    CCCACTGCTG CCAGAGCTCA CCGGCCGCCC CTCCCTCTCC CTCCGACCCC TCCCTCCACA
    GTCCCGGCGG CCCAGCCCTT GGAGTTTGCC CAGTTCCCCT TCCTCCTGCG CGGCCTCCAG
    AAGACTGCAG ACTTCGTGGA GGCCATCGAG GGGGCCCGGG CAGCGTGTGC CGAGGCCGGC
    CAGGCCGGGG TGCATGCCTA CCCCAGCGGC TCCCCCTTCC TCTTCTGGGA GCAGTACCTG
    AGCCTGCGGC GCTGCTTCCT GCTAGCGGTC TGCATCCTGC TGCTGTGTAC TTTCCTCGTC
    TGTGCCCTGC TGCTGCTCAA CCCCTGGACG GCTGTGCTCA TAGTACTGGT CCTGGCGATG
    ATGACTGTGG AGCTCTTTGG CATCATGGGT TTCCTGGGCA TCAAGCTGAG TGCCATCCCG
    GTGGTGATTC TTGTGGCCTC CATAGGCATC GGTGTCGAGT TCACTGTCCA CGTGGCTCTG
    GGCTTCCTGA CCGCTCAGGG TAGCCGGAAC CTGCGGGCTG CCCGGGCCCT GGAGCGCACA
    TTTGCCCCAG TGACCGATGG GGCCATCTCC ACGTTGCTAG GTCTGCTCAT GCTTGCTGGC
    TCCAACTTTG ACTTCATCGT AAGGTACTTC TTTGTGGTGC TGACAATACT TACACTCCTG
    GGCCTCCTCC ACGGGCTCGT GCTGCTGCCG GTACTGCTGT CCATCCTGGG CCCCCCACCA
    GAGGTGGTAC AGATGTACAA GGAGAGCACA GAGGTCCTGA GCCCCCCAGC TCCACAGGGA
    GGAGGGCTTA GGTGGGGGGT ACCCTCCACC CTGCCCCAGA GCTTTGCCAG AGTGACTACC
    TCCATGACTG TGGCCCTCCA CCCACCCCCA CTGCCTGGTG CCTACATCCA CCCAGCCTCT
    GAGGAGCCTA CTTGGTCACC TGCTGCCACA CCAGCTGCCA ATGGCCCCAG CAACCTCGGG
    TCTAGGGGAC TGTGTCCAGC CACGGGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu68864
    Clone ID Related Accession (Same CDS sequence) XM_006052578.2
    Accession Version XM_006052578.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3750bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product protein patched homolog 2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037550.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006052578.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCTCGGC CGCCACCTCT CGGGGAGCTG CCCCCGGGCT ACACACCCCC AGCTCGACCT 
    GCAACACCCC AGATCCTAGC TGGGAGCCTG AAGGCTCCGC TCTGGCTCCG TGCTTACTTC
    CAGGGCCTGC TCTTCTCTTT GGGCTGCGGG ATCCAGAGAC ACTGTGGCAA AGTGCTCTTC
    CTGGGACTGT TGGCCTTTGG GGCCCTAGCA CTGGGTCTCC GCGTGGCCAT CATTGAGACA
    GACCTAGAAC AGCTCTGGGT GGAAGTGGGC AGCCGGGTGA GCCAGGAACT GGAATACACC
    AAGGAGAAGC TGGGGGAGGA GGCTGCGTAT ACCTCCCAGA TGTTGATACA GACCCCGCGT
    CAGGAGGGGG AGAACGTCCT CACGCCCGAG GCACTTGACC TCCACCTCCA GGCAGCCCTC
    ACCGCCAGTA AAGTGTCCTG GGATCTGAAC AAAATCTGCT ACAAGTCAGG AGTTCCCCTA
    ATTGAAAATG GAATGATTGA GCGGATGATT GAAAAGCTGT TTCCCTGCGT GATCCTCACC
    CCCCTCGACT GCTTCTGGGA GGGAGCCAAA CTCCAAGGGG GCTCTGCCTA CTTGCCGGGC
    CGCCCAGACA TCCAGTGGAC CAACCTGGAC CCAGAGCAGC TGCTTGAGGA GCTGGGCCCC
    TTTGCCTCCC TTGAGGGCTT CCGGGAGCTG CTAGACAAGG CACAGGTGGG CCAGGCCTAC
    GTGGGTCGGC CCTGTCTGCA CCCTGACGAC CTCCACTGCC CACCTAGTGC CCCTAACCAT
    CACAGCAAGC AGGCTCCCAA CGTGGCTCAG GAGTTGAGTG GGGGCTGCCA TGGCTTCTCC
    CACAAGTTTA TGCACTGGCA GGAGGAGCTG CTGCTGGGAG GCATGGCCAG AGACCCCCAA
    GGACAGCTGC TGAGGGCAGA GGCCCTGCAG AGCACCTTCC TGCTAATGAG CCCCCGCCAG
    CTCTACGAGC ACTTCCGAGG TGACTACCAG ACGCATGACA TCGGCTGGAG CGAGGAGCAG
    GCCGGCACGG TCCTTCAGGC CTGGCAGCGA CGCTTTGTGC AGCTGGCCCA GGAGGCCCTG
    CCCGAGAACG CATCCCAGCA GATTCACGCC TTCTCCTCTA CCACCCTGGA TGACATCCTG
    CACGCCTTCT CTGAAGTCAG CGCTGCTCGC GTGGCGGGGG GCTACCTGCT CATGCTAGCC
    TATGCCTGTG TGACGATGCT GCGGTGGGAC TGTGCTCAGT CCCAGGGTGC AGTGGGCCTC
    GCTGGTGTGC TGCTGGTGGC CCTGGCAGTG GCCTCGGGCC TGGGGCTCTG TGCCCTGTTG
    GGCATCGCCT TCAACGCTGC CACTACCCAG GTGCTGCCTT TCTTGGCACT GGGCATCGGC
    GTGGATGACA TATTCCTGCT GGCACACGCC TTCACAGAGG CTCCACCTGG CACCCCTCTC
    CAGGAGCGCA CGGGGGAGTG TCTGCGGCGC ACAGGCACCG GTGTCACACT CACATCCGTC
    AACAATATGG TCGCCTTCTT CATGGCCGCC CTAGTCCCCA TCCCTGCACT TCGGGCCTTC
    TCCTTGCAGG CAGCCATAGT GGTGGGCTGC AACTTTGCAG CCGTGATGCT TGTCTTCCCA
    GCGGTCCTCA GCCTGGACCT GCAGCGGCGC CACTGCCGGC GCCTGGATGT GCTCTGCTGC
    TTCTCTAGCC CCTGCTCTGC TCAGGTGATT CAGATTCTGC CCCAGGAGCT GGGGAACGGG
    ACGGTACCAG TGGGCGTTGC CCACCTGACT GCCACGGTTC AAGCCTTTGC CCACTGTGAA
    GCCAGGAGCC AACATGTGGT CACCATCTTG CCTCCCCAAG CTCACCTGGT GCCGCCACCT
    TCTGACCCTT TGGGCTCTGA GCTCTTCAGC CCAGGAGGGT CCACACGGGA CCTTCTAGGG
    CAGGAGGAGG GGACAGGGCA GAAGGCAGCC TGCAAGTCCC TGCCCTGTGC CCGCTGGAAT
    CTTGCCCATT TCGCCCGCTC TCAGTTTGCA CCCTTGTTGC TCCAGTCCCC CACCAAGCTT
    AGTCACCTTT CCCCACAGGC TGTAGTACTG GTACTTTTTG GGGCTCTCCT GGGCCTGAGC
    CTCTATGGAG CGACCTTGGT GCAGGACGGT CTGGCCCTGA CTGATGTGGT GCCGCGTGGC
    ACCAAGGAGC ATGCCTTCCT GAGCGCCCAG CTCAGGTACT TCTCTCTGTA CGAGGTGGCT
    CTGGTGACAC AGGGTGGCTT TGACTACGCC CACTCCCAAC GCGCCCTCTT TGATCTGCAC
    CAGCGCTTCA GTTCCCTCAA GGCCGTGCTG CCCCCACCGG CCACCCAGGC ACCCCGCACC
    TGGCTGCACT ACTACCGCAG CTGGCTACAG GGAATCCAGG CTGCGTTTGA CCAGGACTGG
    GCTTCTGGGC GCATCACCCG CCACTCATAC CGCAATGGCT CTGAGGATGG GGCCCTGGCA
    TACAAGCTGC TTGTCCAGAC CGGGGATGCC CAGGAGCCTC TAGATTTCAG CCAGCTGACC
    ACCAAGAAGC TGGTGGACAA GGAAGGGCTG ATCCCACCCG AGCTCTTCTA CATGGGGCTG
    ACCGTGTGGG TGAGCAGTGA CCCCCTGGGT CTGGCAGCCT CACAGGCCAA CTTCTATCCC
    CCACCTCCTG AGTGGCTGCA TGACAAGTAC GATACCACGG GGGAGAATCT TCGCAGTGAG
    TTCTGGGGGA GCCCGAACTG CCTGGGCCCA AGCCCACCCA GGCCCTCCCC TGAAGCCCTG
    CCCACTGCTG CCAGAGCTCA CCGGCCGCCC CTCCCTCTCC CTCCGACCCC TCCCTCCACA
    GTCCCGGCGG CCCAGCCCTT GGAGTTTGCC CAGTTCCCCT TCCTCCTGCG CGGCCTCCAG
    AAGACTGCAG ACTTCGTGGA GGCCATCGAG GGGGCCCGGG CAGCGTGTGC CGAGGCCGGC
    CAGGCCGGGG TGCATGCCTA CCCCAGCGGC TCCCCCTTCC TCTTCTGGGA GCAGTACCTG
    AGCCTGCGGC GCTGCTTCCT GCTAGCGGTC TGCATCCTGC TGCTGTGTAC TTTCCTCGTC
    TGTGCCCTGC TGCTGCTCAA CCCCTGGACG GCTGTGCTCA TAGTACTGGT CCTGGCGATG
    ATGACTGTGG AGCTCTTTGG CATCATGGGT TTCCTGGGCA TCAAGCTGAG TGCCATCCCG
    GTGGTGATTC TTGTGGCCTC CATAGGCATC GGTGTCGAGT TCACTGTCCA CGTGGCTCTG
    GGCTTCCTGA CCGCTCAGGG TAGCCGGAAC CTGCGGGCTG CCCGGGCCCT GGAGCGCACA
    TTTGCCCCAG TGACCGATGG GGCCATCTCC ACGTTGCTAG GTCTGCTCAT GCTTGCTGGC
    TCCAACTTTG ACTTCATCGT AAGGTACTTC TTTGTGGTGC TGACAATACT TACACTCCTG
    GGCCTCCTCC ACGGGCTCGT GCTGCTGCCG GTACTGCTGT CCATCCTGGG CCCCCCACCA
    GAGGTGGTAC AGATGTACAA GGAGAGCACA GAGGTCCTGA GCCCCCCAGC TCCACAGGGA
    GGAGGGCTTA GGTGGGGGGT ACCCTCCACC CTGCCCCAGA GCTTTGCCAG AGTGACTACC
    TCCATGACTG TGGCCCTCCA CCCACCCCCA CTGCCTGGTG CCTACATCCA CCCAGCCTCT
    GAGGAGCCTA CTTGGTCACC TGCTGCCACA CCAGCTGCCA ATGGCCCCAG CAACCTCGGG
    TCTAGGGGAC TGTGTCCAGC CACGGGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006052640.2
    CDS434..4183
    Translation

    Target ORF information:

    RefSeq Version XM_006052578.2
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis patched 2 (PTCH2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006052578.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCTCGGC CGCCACCTCT CGGGGAGCTG CCCCCGGGCT ACACACCCCC AGCTCGACCT 
    GCAACACCCC AGATCCTAGC TGGGAGCCTG AAGGCTCCGC TCTGGCTCCG TGCTTACTTC
    CAGGGCCTGC TCTTCTCTTT GGGCTGCGGG ATCCAGAGAC ACTGTGGCAA AGTGCTCTTC
    CTGGGACTGT TGGCCTTTGG GGCCCTAGCA CTGGGTCTCC GCGTGGCCAT CATTGAGACA
    GACCTAGAAC AGCTCTGGGT GGAAGTGGGC AGCCGGGTGA GCCAGGAACT GGAATACACC
    AAGGAGAAGC TGGGGGAGGA GGCTGCGTAT ACCTCCCAGA TGTTGATACA GACCCCGCGT
    CAGGAGGGGG AGAACGTCCT CACGCCCGAG GCACTTGACC TCCACCTCCA GGCAGCCCTC
    ACCGCCAGTA AAGTGTCCTG GGATCTGAAC AAAATCTGCT ACAAGTCAGG AGTTCCCCTA
    ATTGAAAATG GAATGATTGA GCGGATGATT GAAAAGCTGT TTCCCTGCGT GATCCTCACC
    CCCCTCGACT GCTTCTGGGA GGGAGCCAAA CTCCAAGGGG GCTCTGCCTA CTTGCCGGGC
    CGCCCAGACA TCCAGTGGAC CAACCTGGAC CCAGAGCAGC TGCTTGAGGA GCTGGGCCCC
    TTTGCCTCCC TTGAGGGCTT CCGGGAGCTG CTAGACAAGG CACAGGTGGG CCAGGCCTAC
    GTGGGTCGGC CCTGTCTGCA CCCTGACGAC CTCCACTGCC CACCTAGTGC CCCTAACCAT
    CACAGCAAGC AGGCTCCCAA CGTGGCTCAG GAGTTGAGTG GGGGCTGCCA TGGCTTCTCC
    CACAAGTTTA TGCACTGGCA GGAGGAGCTG CTGCTGGGAG GCATGGCCAG AGACCCCCAA
    GGACAGCTGC TGAGGGCAGA GGCCCTGCAG AGCACCTTCC TGCTAATGAG CCCCCGCCAG
    CTCTACGAGC ACTTCCGAGG TGACTACCAG ACGCATGACA TCGGCTGGAG CGAGGAGCAG
    GCCGGCACGG TCCTTCAGGC CTGGCAGCGA CGCTTTGTGC AGCTGGCCCA GGAGGCCCTG
    CCCGAGAACG CATCCCAGCA GATTCACGCC TTCTCCTCTA CCACCCTGGA TGACATCCTG
    CACGCCTTCT CTGAAGTCAG CGCTGCTCGC GTGGCGGGGG GCTACCTGCT CATGCTAGCC
    TATGCCTGTG TGACGATGCT GCGGTGGGAC TGTGCTCAGT CCCAGGGTGC AGTGGGCCTC
    GCTGGTGTGC TGCTGGTGGC CCTGGCAGTG GCCTCGGGCC TGGGGCTCTG TGCCCTGTTG
    GGCATCGCCT TCAACGCTGC CACTACCCAG GTGCTGCCTT TCTTGGCACT GGGCATCGGC
    GTGGATGACA TATTCCTGCT GGCACACGCC TTCACAGAGG CTCCACCTGG CACCCCTCTC
    CAGGAGCGCA CGGGGGAGTG TCTGCGGCGC ACAGGCACCG GTGTCACACT CACATCCGTC
    AACAATATGG TCGCCTTCTT CATGGCCGCC CTAGTCCCCA TCCCTGCACT TCGGGCCTTC
    TCCTTGCAGG CAGCCATAGT GGTGGGCTGC AACTTTGCAG CCGTGATGCT TGTCTTCCCA
    GCGGTCCTCA GCCTGGACCT GCAGCGGCGC CACTGCCGGC GCCTGGATGT GCTCTGCTGC
    TTCTCTAGCC CCTGCTCTGC TCAGGTGATT CAGATTCTGC CCCAGGAGCT GGGGAACGGG
    ACGGTACCAG TGGGCGTTGC CCACCTGACT GCCACGGTTC AAGCCTTTGC CCACTGTGAA
    GCCAGGAGCC AACATGTGGT CACCATCTTG CCTCCCCAAG CTCACCTGGT GCCGCCACCT
    TCTGACCCTT TGGGCTCTGA GCTCTTCAGC CCAGGAGGGT CCACACGGGA CCTTCTAGGG
    CAGGAGGAGG GGACAGGGCA GAAGGCAGCC TGCAAGTCCC TGCCCTGTGC CCGCTGGAAT
    CTTGCCCATT TCGCCCGCTC TCAGTTTGCA CCCTTGTTGC TCCAGTCCCC CACCAAGCTT
    AGTCACCTTT CCCCACAGGC TGTAGTACTG GTACTTTTTG GGGCTCTCCT GGGCCTGAGC
    CTCTATGGAG CGACCTTGGT GCAGGACGGT CTGGCCCTGA CTGATGTGGT GCCGCGTGGC
    ACCAAGGAGC ATGCCTTCCT GAGCGCCCAG CTCAGGTACT TCTCTCTGTA CGAGGTGGCT
    CTGGTGACAC AGGGTGGCTT TGACTACGCC CACTCCCAAC GCGCCCTCTT TGATCTGCAC
    CAGCGCTTCA GTTCCCTCAA GGCCGTGCTG CCCCCACCGG CCACCCAGGC ACCCCGCACC
    TGGCTGCACT ACTACCGCAG CTGGCTACAG GGAATCCAGG CTGCGTTTGA CCAGGACTGG
    GCTTCTGGGC GCATCACCCG CCACTCATAC CGCAATGGCT CTGAGGATGG GGCCCTGGCA
    TACAAGCTGC TTGTCCAGAC CGGGGATGCC CAGGAGCCTC TAGATTTCAG CCAGCTGACC
    ACCAAGAAGC TGGTGGACAA GGAAGGGCTG ATCCCACCCG AGCTCTTCTA CATGGGGCTG
    ACCGTGTGGG TGAGCAGTGA CCCCCTGGGT CTGGCAGCCT CACAGGCCAA CTTCTATCCC
    CCACCTCCTG AGTGGCTGCA TGACAAGTAC GATACCACGG GGGAGAATCT TCGCAGTGAG
    TTCTGGGGGA GCCCGAACTG CCTGGGCCCA AGCCCACCCA GGCCCTCCCC TGAAGCCCTG
    CCCACTGCTG CCAGAGCTCA CCGGCCGCCC CTCCCTCTCC CTCCGACCCC TCCCTCCACA
    GTCCCGGCGG CCCAGCCCTT GGAGTTTGCC CAGTTCCCCT TCCTCCTGCG CGGCCTCCAG
    AAGACTGCAG ACTTCGTGGA GGCCATCGAG GGGGCCCGGG CAGCGTGTGC CGAGGCCGGC
    CAGGCCGGGG TGCATGCCTA CCCCAGCGGC TCCCCCTTCC TCTTCTGGGA GCAGTACCTG
    AGCCTGCGGC GCTGCTTCCT GCTAGCGGTC TGCATCCTGC TGCTGTGTAC TTTCCTCGTC
    TGTGCCCTGC TGCTGCTCAA CCCCTGGACG GCTGTGCTCA TAGTACTGGT CCTGGCGATG
    ATGACTGTGG AGCTCTTTGG CATCATGGGT TTCCTGGGCA TCAAGCTGAG TGCCATCCCG
    GTGGTGATTC TTGTGGCCTC CATAGGCATC GGTGTCGAGT TCACTGTCCA CGTGGCTCTG
    GGCTTCCTGA CCGCTCAGGG TAGCCGGAAC CTGCGGGCTG CCCGGGCCCT GGAGCGCACA
    TTTGCCCCAG TGACCGATGG GGCCATCTCC ACGTTGCTAG GTCTGCTCAT GCTTGCTGGC
    TCCAACTTTG ACTTCATCGT AAGGTACTTC TTTGTGGTGC TGACAATACT TACACTCCTG
    GGCCTCCTCC ACGGGCTCGT GCTGCTGCCG GTACTGCTGT CCATCCTGGG CCCCCCACCA
    GAGGTGGTAC AGATGTACAA GGAGAGCACA GAGGTCCTGA GCCCCCCAGC TCCACAGGGA
    GGAGGGCTTA GGTGGGGGGT ACCCTCCACC CTGCCCCAGA GCTTTGCCAG AGTGACTACC
    TCCATGACTG TGGCCCTCCA CCCACCCCCA CTGCCTGGTG CCTACATCCA CCCAGCCTCT
    GAGGAGCCTA CTTGGTCACC TGCTGCCACA CCAGCTGCCA ATGGCCCCAG CAACCTCGGG
    TCTAGGGGAC TGTGTCCAGC CACGGGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu68865
    Clone ID Related Accession (Same CDS sequence) XM_025288853.1
    Accession Version XM_025288853.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3720bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product protein patched homolog 2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037550.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCTCGGC CGCCACCTCT CGGGGAGCTG CCCCCGGGCT ACACACCCCC AGCTCGACCT 
    GCAACACCCC AGGGCCTGCT CTTCTCTTTG GGCTGCGGGA TCCAGAGACA CTGTGGCAAA
    GTGCTCTTCC TGGGACTGTT GGCCTTTGGG GCCCTAGCAC TGGGTCTCCG CGTGGCCATC
    ATTGAGACAG ACCTAGAACA GCTCTGGGTG GAAGTGGGCA GCCGGGTGAG CCAGGAACTG
    GAATACACCA AGGAGAAGCT GGGGGAGGAG GCTGCGTATA CCTCCCAGAT GTTGATACAG
    ACCCCGCGTC AGGAGGGGGA GAACGTCCTC ACGCCCGAGG CACTTGACCT CCACCTCCAG
    GCAGCCCTCA CCGCCAGTAA AGTGCAAGTA TCCCTCTATG GAAAGTCCTG GGATCTGAAC
    AAAATCTGCT ACAAGTCAGG AGTTCCCCTA ATTGAAAATG GAATGATTGA GCGGATGATT
    GAAAAGCTGT TTCCCTGCGT GATCCTCACC CCCCTCGACT GCTTCTGGGA GGGAGCCAAA
    CTCCAAGGGG GCTCTGCCTA CTTGCCGGGC CGCCCAGACA TCCAGTGGAC CAACCTGGAC
    CCAGAGCAGC TGCTTGAGGA GCTGGGCCCC TTTGCCTCCC TTGAGGGCTT CCGGGAGCTG
    CTAGACAAGG CACAGGTGGG CCAGGCCTAC GTGGGTCGGC CCTGTCTGCA CCCTGACGAC
    CTCCACTGCC CACCTAGTGC CCCTAACCAT CACAGCAAGC AGGCTCCCAA CGTGGCTCAG
    GAGTTGAGTG GGGGCTGCCA TGGCTTCTCC CACAAGTTTA TGCACTGGCA GGAGGAGCTG
    CTGCTGGGAG GCATGGCCAG AGACCCCCAA GGACAGCTGC TGAGGGCAGA GGCCCTGCAG
    AGCACCTTCC TGCTAATGAG CCCCCGCCAG CTCTACGAGC ACTTCCGAGG TGACTACCAG
    ACGCATGACA TCGGCTGGAG CGAGGAGCAG GCCGGCACGG TCCTTCAGGC CTGGCAGCGA
    CGCTTTGTGC AGCTGGCCCA GGAGGCCCTG CCCGAGAACG CATCCCAGCA GATTCACGCC
    TTCTCCTCTA CCACCCTGGA TGACATCCTG CACGCCTTCT CTGAAGTCAG CGCTGCTCGC
    GTGGCGGGGG GCTACCTGCT CATGCTAGCC TATGCCTGTG TGACGATGCT GCGGTGGGAC
    TGTGCTCAGT CCCAGGGTGC AGTGGGCCTC GCTGGTGTGC TGCTGGTGGC CCTGGCAGTG
    GCCTCGGGCC TGGGGCTCTG TGCCCTGTTG GGCATCGCCT TCAACGCTGC CACTACCCAG
    GTGCTGCCTT TCTTGGCACT GGGCATCGGC GTGGATGACA TATTCCTGCT GGCACACGCC
    TTCACAGAGG CTCCACCTGG CACCCCTCTC CAGGAGCGCA CGGGGGAGTG TCTGCGGCGC
    ACAGGCACCG GTGTCACACT CACATCCGTC AACAATATGG TCGCCTTCTT CATGGCCGCC
    CTAGTCCCCA TCCCTGCACT TCGGGCCTTC TCCTTGCAGG CAGCCATAGT GGTGGGCTGC
    AACTTTGCAG CCGTGATGCT TGTCTTCCCA GCGGTCCTCA GCCTGGACCT GCAGCGGCGC
    CACTGCCGGC GCCTGGATGT GCTCTGCTGC TTCTCTAGCC CCTGCTCTGC TCAGGTGATT
    CAGATTCTGC CCCAGGAGCT GGGGAACGGG ACGGTACCAG TGGGCGTTGC CCACCTGACT
    GCCACGGTTC AAGCCTTTGC CCACTGTGAA GCCAGGAGCC AACATGTGGT CACCATCTTG
    CCTCCCCAAG CTCACCTGGT GCCGCCACCT TCTGACCCTT TGGGCTCTGA GCTCTTCAGC
    CCAGGAGGGT CCACACGGGA CCTTCTAGGG CAGGAGGAGG GGACAGGGCA GAAGGCAGCC
    TGCAAGTCCC TGCCCTGTGC CCGCTGGAAT CTTGCCCATT TCGCCCGCTC TCAGTTTGCA
    CCCTTGTTGC TCCAGTCCCC CACCAAGCTT AGTCACCTTT CCCCACAGGC TGTAGTACTG
    GTACTTTTTG GGGCTCTCCT GGGCCTGAGC CTCTATGGAG CGACCTTGGT GCAGGACGGT
    CTGGCCCTGA CTGATGTGGT GCCGCGTGGC ACCAAGGAGC ATGCCTTCCT GAGCGCCCAG
    CTCAGGTACT TCTCTCTGTA CGAGGTGGCT CTGGTGACAC AGGGTGGCTT TGACTACGCC
    CACTCCCAAC GCGCCCTCTT TGATCTGCAC CAGCGCTTCA GTTCCCTCAA GGCCGTGCTG
    CCCCCACCGG CCACCCAGGC ACCCCGCACC TGGCTGCACT ACTACCGCAG CTGGCTACAG
    GGAATCCAGG CTGCGTTTGA CCAGGACTGG GCTTCTGGGC GCATCACCCG CCACTCATAC
    CGCAATGGCT CTGAGGATGG GGCCCTGGCA TACAAGCTGC TTGTCCAGAC CGGGGATGCC
    CAGGAGCCTC TAGATTTCAG CCAGCTGACC ACCAAGAAGC TGGTGGACAA GGAAGGGCTG
    ATCCCACCCG AGCTCTTCTA CATGGGGCTG ACCGTGTGGG TGAGCAGTGA CCCCCTGGGT
    CTGGCAGCCT CACAGGCCAA CTTCTATCCC CCACCTCCTG AGTGGCTGCA TGACAAGTAC
    GATACCACGG GGGAGAATCT TCGCAGTGAG TTCTGGGGGA GCCCGAACTG CCTGGGCCCA
    AGCCCACCCA GGCCCTCCCC TGAAGCCCTG CCCACTGCTG CCAGAGCTCA CCGGCCGCCC
    CTCCCTCTCC CTCCGACCCC TCCCTCCACA GTCCCGGCGG CCCAGCCCTT GGAGTTTGCC
    CAGTTCCCCT TCCTCCTGCG CGGCCTCCAG AAGACTGCAG ACTTCGTGGA GGCCATCGAG
    GGGGCCCGGG CAGCGTGTGC CGAGGCCGGC CAGGCCGGGG TGCATGCCTA CCCCAGCGGC
    TCCCCCTTCC TCTTCTGGGA GCAGTACCTG AGCCTGCGGC GCTGCTTCCT GCTAGCGGTC
    TGCATCCTGC TGCTGTGTAC TTTCCTCGTC TGTGCCCTGC TGCTGCTCAA CCCCTGGACG
    GCTGTGCTCA TAGTACTGGT CCTGGCGATG ATGACTGTGG AGCTCTTTGG CATCATGGGT
    TTCCTGGGCA TCAAGCTGAG TGCCATCCCG GTGGTGATTC TTGTGGCCTC CATAGGCATC
    GGTGTCGAGT TCACTGTCCA CGTGGCTCTG GGCTTCCTGA CCGCTCAGGG TAGCCGGAAC
    CTGCGGGCTG CCCGGGCCCT GGAGCGCACA TTTGCCCCAG TGACCGATGG GGCCATCTCC
    ACGTTGCTAG GTCTGCTCAT GCTTGCTGGC TCCAACTTTG ACTTCATCGT AAGGTACTTC
    TTTGTGGTGC TGACAATACT TACACTCCTG GGCCTCCTCC ACGGGCTCGT GCTGCTGCCG
    GTACTGCTGT CCATCCTGGG CCCCCCACCA GAGGTGGTAC AGATGTACAA GGAGAGCACA
    GAGGTCCTGA GCCCCCCAGC TCCACAGGGA GGAGGGCTTA GGTGGGGGGT ACCCTCCACC
    CTGCCCCAGA GCTTTGCCAG AGTGACTACC TCCATGACTG TGGCCCTCCA CCCACCCCCA
    CTGCCTGGTG CCTACATCCA CCCAGCCTCT GAGGAGCCTA CTTGGTCACC TGCTGCCACA
    CCAGCTGCCA ATGGCCCCAG CAACCTCGGG TCTAGGGGAC TGTGTCCAGC CACGGGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025144638.1
    CDS433..4152
    Translation

    Target ORF information:

    RefSeq Version XM_025288853.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis patched 2 (PTCH2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025288853.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCTCGGC CGCCACCTCT CGGGGAGCTG CCCCCGGGCT ACACACCCCC AGCTCGACCT 
    GCAACACCCC AGGGCCTGCT CTTCTCTTTG GGCTGCGGGA TCCAGAGACA CTGTGGCAAA
    GTGCTCTTCC TGGGACTGTT GGCCTTTGGG GCCCTAGCAC TGGGTCTCCG CGTGGCCATC
    ATTGAGACAG ACCTAGAACA GCTCTGGGTG GAAGTGGGCA GCCGGGTGAG CCAGGAACTG
    GAATACACCA AGGAGAAGCT GGGGGAGGAG GCTGCGTATA CCTCCCAGAT GTTGATACAG
    ACCCCGCGTC AGGAGGGGGA GAACGTCCTC ACGCCCGAGG CACTTGACCT CCACCTCCAG
    GCAGCCCTCA CCGCCAGTAA AGTGCAAGTA TCCCTCTATG GAAAGTCCTG GGATCTGAAC
    AAAATCTGCT ACAAGTCAGG AGTTCCCCTA ATTGAAAATG GAATGATTGA GCGGATGATT
    GAAAAGCTGT TTCCCTGCGT GATCCTCACC CCCCTCGACT GCTTCTGGGA GGGAGCCAAA
    CTCCAAGGGG GCTCTGCCTA CTTGCCGGGC CGCCCAGACA TCCAGTGGAC CAACCTGGAC
    CCAGAGCAGC TGCTTGAGGA GCTGGGCCCC TTTGCCTCCC TTGAGGGCTT CCGGGAGCTG
    CTAGACAAGG CACAGGTGGG CCAGGCCTAC GTGGGTCGGC CCTGTCTGCA CCCTGACGAC
    CTCCACTGCC CACCTAGTGC CCCTAACCAT CACAGCAAGC AGGCTCCCAA CGTGGCTCAG
    GAGTTGAGTG GGGGCTGCCA TGGCTTCTCC CACAAGTTTA TGCACTGGCA GGAGGAGCTG
    CTGCTGGGAG GCATGGCCAG AGACCCCCAA GGACAGCTGC TGAGGGCAGA GGCCCTGCAG
    AGCACCTTCC TGCTAATGAG CCCCCGCCAG CTCTACGAGC ACTTCCGAGG TGACTACCAG
    ACGCATGACA TCGGCTGGAG CGAGGAGCAG GCCGGCACGG TCCTTCAGGC CTGGCAGCGA
    CGCTTTGTGC AGCTGGCCCA GGAGGCCCTG CCCGAGAACG CATCCCAGCA GATTCACGCC
    TTCTCCTCTA CCACCCTGGA TGACATCCTG CACGCCTTCT CTGAAGTCAG CGCTGCTCGC
    GTGGCGGGGG GCTACCTGCT CATGCTAGCC TATGCCTGTG TGACGATGCT GCGGTGGGAC
    TGTGCTCAGT CCCAGGGTGC AGTGGGCCTC GCTGGTGTGC TGCTGGTGGC CCTGGCAGTG
    GCCTCGGGCC TGGGGCTCTG TGCCCTGTTG GGCATCGCCT TCAACGCTGC CACTACCCAG
    GTGCTGCCTT TCTTGGCACT GGGCATCGGC GTGGATGACA TATTCCTGCT GGCACACGCC
    TTCACAGAGG CTCCACCTGG CACCCCTCTC CAGGAGCGCA CGGGGGAGTG TCTGCGGCGC
    ACAGGCACCG GTGTCACACT CACATCCGTC AACAATATGG TCGCCTTCTT CATGGCCGCC
    CTAGTCCCCA TCCCTGCACT TCGGGCCTTC TCCTTGCAGG CAGCCATAGT GGTGGGCTGC
    AACTTTGCAG CCGTGATGCT TGTCTTCCCA GCGGTCCTCA GCCTGGACCT GCAGCGGCGC
    CACTGCCGGC GCCTGGATGT GCTCTGCTGC TTCTCTAGCC CCTGCTCTGC TCAGGTGATT
    CAGATTCTGC CCCAGGAGCT GGGGAACGGG ACGGTACCAG TGGGCGTTGC CCACCTGACT
    GCCACGGTTC AAGCCTTTGC CCACTGTGAA GCCAGGAGCC AACATGTGGT CACCATCTTG
    CCTCCCCAAG CTCACCTGGT GCCGCCACCT TCTGACCCTT TGGGCTCTGA GCTCTTCAGC
    CCAGGAGGGT CCACACGGGA CCTTCTAGGG CAGGAGGAGG GGACAGGGCA GAAGGCAGCC
    TGCAAGTCCC TGCCCTGTGC CCGCTGGAAT CTTGCCCATT TCGCCCGCTC TCAGTTTGCA
    CCCTTGTTGC TCCAGTCCCC CACCAAGCTT AGTCACCTTT CCCCACAGGC TGTAGTACTG
    GTACTTTTTG GGGCTCTCCT GGGCCTGAGC CTCTATGGAG CGACCTTGGT GCAGGACGGT
    CTGGCCCTGA CTGATGTGGT GCCGCGTGGC ACCAAGGAGC ATGCCTTCCT GAGCGCCCAG
    CTCAGGTACT TCTCTCTGTA CGAGGTGGCT CTGGTGACAC AGGGTGGCTT TGACTACGCC
    CACTCCCAAC GCGCCCTCTT TGATCTGCAC CAGCGCTTCA GTTCCCTCAA GGCCGTGCTG
    CCCCCACCGG CCACCCAGGC ACCCCGCACC TGGCTGCACT ACTACCGCAG CTGGCTACAG
    GGAATCCAGG CTGCGTTTGA CCAGGACTGG GCTTCTGGGC GCATCACCCG CCACTCATAC
    CGCAATGGCT CTGAGGATGG GGCCCTGGCA TACAAGCTGC TTGTCCAGAC CGGGGATGCC
    CAGGAGCCTC TAGATTTCAG CCAGCTGACC ACCAAGAAGC TGGTGGACAA GGAAGGGCTG
    ATCCCACCCG AGCTCTTCTA CATGGGGCTG ACCGTGTGGG TGAGCAGTGA CCCCCTGGGT
    CTGGCAGCCT CACAGGCCAA CTTCTATCCC CCACCTCCTG AGTGGCTGCA TGACAAGTAC
    GATACCACGG GGGAGAATCT TCGCAGTGAG TTCTGGGGGA GCCCGAACTG CCTGGGCCCA
    AGCCCACCCA GGCCCTCCCC TGAAGCCCTG CCCACTGCTG CCAGAGCTCA CCGGCCGCCC
    CTCCCTCTCC CTCCGACCCC TCCCTCCACA GTCCCGGCGG CCCAGCCCTT GGAGTTTGCC
    CAGTTCCCCT TCCTCCTGCG CGGCCTCCAG AAGACTGCAG ACTTCGTGGA GGCCATCGAG
    GGGGCCCGGG CAGCGTGTGC CGAGGCCGGC CAGGCCGGGG TGCATGCCTA CCCCAGCGGC
    TCCCCCTTCC TCTTCTGGGA GCAGTACCTG AGCCTGCGGC GCTGCTTCCT GCTAGCGGTC
    TGCATCCTGC TGCTGTGTAC TTTCCTCGTC TGTGCCCTGC TGCTGCTCAA CCCCTGGACG
    GCTGTGCTCA TAGTACTGGT CCTGGCGATG ATGACTGTGG AGCTCTTTGG CATCATGGGT
    TTCCTGGGCA TCAAGCTGAG TGCCATCCCG GTGGTGATTC TTGTGGCCTC CATAGGCATC
    GGTGTCGAGT TCACTGTCCA CGTGGCTCTG GGCTTCCTGA CCGCTCAGGG TAGCCGGAAC
    CTGCGGGCTG CCCGGGCCCT GGAGCGCACA TTTGCCCCAG TGACCGATGG GGCCATCTCC
    ACGTTGCTAG GTCTGCTCAT GCTTGCTGGC TCCAACTTTG ACTTCATCGT AAGGTACTTC
    TTTGTGGTGC TGACAATACT TACACTCCTG GGCCTCCTCC ACGGGCTCGT GCTGCTGCCG
    GTACTGCTGT CCATCCTGGG CCCCCCACCA GAGGTGGTAC AGATGTACAA GGAGAGCACA
    GAGGTCCTGA GCCCCCCAGC TCCACAGGGA GGAGGGCTTA GGTGGGGGGT ACCCTCCACC
    CTGCCCCAGA GCTTTGCCAG AGTGACTACC TCCATGACTG TGGCCCTCCA CCCACCCCCA
    CTGCCTGGTG CCTACATCCA CCCAGCCTCT GAGGAGCCTA CTTGGTCACC TGCTGCCACA
    CCAGCTGCCA ATGGCCCCAG CAACCTCGGG TCTAGGGGAC TGTGTCCAGC CACGGGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu68866
    Clone ID Related Accession (Same CDS sequence) XM_006052579.2
    Accession Version XM_006052579.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3645bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product protein patched homolog 2 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037550.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006052579.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCTCGGC CGCCACCTCT CGGGGAGCTG CCCCCGGGCT ACACACCCCC AGCTCGACCT 
    GCAACACCCC AGATCCTAGC TGGGAGCCTG AAGGCTCCGC TCTGGCTCCG TGCTTACTTC
    CAGGGCCTGC TCTTCTCTTT GGGCTGCGGG ATCCAGAGAC ACTGTGGCAA AGTGCTCTTC
    CTGGGACTGT TGGCCTTTGG GGCCCTAGCA CTGGGTCTCC GCGTGGCCAT CATTGAGACA
    GACCTAGAAC AGCTCTGGGT GGAAGTGGGC AGCCGGGTGA GCCAGGAACT GGAATACACC
    AAGGAGAAGC TGGGGGAGGA GGCTGCGTAT ACCTCCCAGA TGTTGATACA GACCCCGCGT
    CAGGAGGGGG AGAACGTCCT CACGCCCGAG GCACTTGACC TCCACCTCCA GGCAGCCCTC
    ACCGCCAGTA AAGTGCAAGT ATCCCTCTAT GGAAAGTCCT GGGATCTGAA CAAAATCTGC
    TACAAGTCAG GAGTTCCCCT AATTGAAAAT GGAATGATTG AGCGGATGAT TGAAAAGCTG
    TTTCCCTGCG TGATCCTCAC CCCCCTCGAC TGCTTCTGGG AGGGAGCCAA ACTCCAAGGG
    GGCTCTGCCT ACTTGCCGGG CCGCCCAGAC ATCCAGTGGA CCAACCTGGA CCCAGAGCAG
    CTGCTTGAGG AGCTGGGCCC CTTTGCCTCC CTTGAGGGCT TCCGGGAGCT GCTAGACAAG
    GCACAGGTGG GCCAGGCCTA CGTGGGTCGG CCCTGTCTGC ACCCTGACGA CCTCCACTGC
    CCACCTAGTG CCCCTAACCA TCACAGCAAG CAGGCTCCCA ACGTGGCTCA GGAGTTGAGT
    GGGGGCTGCC ATGGCTTCTC CCACAAGTTT ATGCACTGGC AGGAGGAGCT GCTGCTGGGA
    GGCATGGCCA GAGACCCCCA AGGACAGCTG CTGAGGGCAG AGGCCCTGCA GAGCACCTTC
    CTGCTAATGA GCCCCCGCCA GCTCTACGAG CACTTCCGAG GTGACTACCA GACGCATGAC
    ATCGGCTGGA GCGAGGAGCA GGCCGGCACG GTCCTTCAGG CCTGGCAGCG ACGCTTTGTG
    CAGCTGGCCC AGGAGGCCCT GCCCGAGAAC GCATCCCAGC AGATTCACGC CTTCTCCTCT
    ACCACCCTGG ATGACATCCT GCACGCCTTC TCTGAAGTCA GCGCTGCTCG CGTGGCGGGG
    GGCTACCTGC TCATGCTAGC CTATGCCTGT GTGACGATGC TGCGGTGGGA CTGTGCTCAG
    TCCCAGGGTG CAGTGGGCCT CGCTGGTGTG CTGCTGGTGG CCCTGGCAGT GGCCTCGGGC
    CTGGGGCTCT GTGCCCTGTT GGGCATCGCC TTCAACGCTG CCACTACCCA GGTGCTGCCT
    TTCTTGGCAC TGGGCATCGG CGTGGATGAC ATATTCCTGC TGGCACACGC CTTCACAGAG
    GCTCCACCTG GCACCCCTCT CCAGGAGCGC ACGGGGGAGT GTCTGCGGCG CACAGGCACC
    GGTGTCACAC TCACATCCGT CAACAATATG GTCGCCTTCT TCATGGCCGC CCTAGTCCCC
    ATCCCTGCAC TTCGGGCCTT CTCCTTGCAG GCAGCCATAG TGGTGGGCTG CAACTTTGCA
    GCCGTGATGC TTGTCTTCCC AGCGGTCCTC AGCCTGGACC TGCAGCGGCG CCACTGCCGG
    CGCCTGGATG TGCTCTGCTG CTTCTCTAGC CCCTGCTCTG CTCAGGTGAT TCAGATTCTG
    CCCCAGGAGC TGGGGAACGG GACGGTACCA GTGGGCGTTG CCCACCTGAC TGCCACGGTT
    CAAGCCTTTG CCCACTGTGA AGCCAGGAGC CAACATGTGG TCACCATCTT GCCTCCCCAA
    GCTCACCTGG TGCCGCCACC TTCTGACCCT TTGGGCTCTG AGCTCTTCAG CCCAGGAGGG
    TCCACACGGG ACCTTCTAGG GCAGGAGGAG GGGACAGGGC AGAAGGCAGC CTGCAAGTCC
    CTGCCCTGTG CCCGCTGGAA TCTTGCCCAT TTCGCCCGCT CTCAGTTTGC ACCCTTGTTG
    CTCCAGTCCC CCACCAAGCT TAGTCACCTT TCCCCACAGG CTGTAGTACT GGTACTTTTT
    GGGGCTCTCC TGGGCCTGAG CCTCTATGGA GCGACCTTGG TGCAGGACGG TCTGGCCCTG
    ACTGATGTGG TGCCGCGTGG CACCAAGGAG CATGCCTTCC TGAGCGCCCA GCTCAGGTAC
    TTCTCTCTGT ACGAGGTGGC TCTGGTGACA CAGGGTGGCT TTGACTACGC CCACTCCCAA
    CGCGCCCTCT TTGATCTGCA CCAGCGCTTC AGTTCCCTCA AGGCCGTGCT GCCCCCACCG
    GCCACCCAGG CACCCCGCAC CTGGCTGCAC TACTACCGCA GCTGGCTACA GGGAATCCAG
    GCTGCGTTTG ACCAGGACTG GGCTTCTGGG CGCATCACCC GCCACTCATA CCGCAATGGC
    TCTGAGGATG GGGCCCTGGC ATACAAGCTG CTTGTCCAGA CCGGGGATGC CCAGGAGCCT
    CTAGATTTCA GCCAGCTGAC CACCAAGAAG CTGGTGGACA AGGAAGGGCT GATCCCACCC
    GAGCTCTTCT ACATGGGGCT GACCGTGTGG GTGAGCAGTG ACCCCCTGGG TCTGGCAGCC
    TCACAGGCCA ACTTCTATCC CCCACCTCCT GAGTGGCTGC ATGACAAGTA CGATACCACG
    GGGGAGAATC TTCGCATCCC GGCGGCCCAG CCCTTGGAGT TTGCCCAGTT CCCCTTCCTC
    CTGCGCGGCC TCCAGAAGAC TGCAGACTTC GTGGAGGCCA TCGAGGGGGC CCGGGCAGCG
    TGTGCCGAGG CCGGCCAGGC CGGGGTGCAT GCCTACCCCA GCGGCTCCCC CTTCCTCTTC
    TGGGAGCAGT ACCTGAGCCT GCGGCGCTGC TTCCTGCTAG CGGTCTGCAT CCTGCTGCTG
    TGTACTTTCC TCGTCTGTGC CCTGCTGCTG CTCAACCCCT GGACGGCTGT GCTCATAGTA
    CTGGTCCTGG CGATGATGAC TGTGGAGCTC TTTGGCATCA TGGGTTTCCT GGGCATCAAG
    CTGAGTGCCA TCCCGGTGGT GATTCTTGTG GCCTCCATAG GCATCGGTGT CGAGTTCACT
    GTCCACGTGG CTCTGGGCTT CCTGACCGCT CAGGGTAGCC GGAACCTGCG GGCTGCCCGG
    GCCCTGGAGC GCACATTTGC CCCAGTGACC GATGGGGCCA TCTCCACGTT GCTAGGTCTG
    CTCATGCTTG CTGGCTCCAA CTTTGACTTC ATCGTAAGGT ACTTCTTTGT GGTGCTGACA
    ATACTTACAC TCCTGGGCCT CCTCCACGGG CTCGTGCTGC TGCCGGTACT GCTGTCCATC
    CTGGGCCCCC CACCAGAGGT GGTACAGATG TACAAGGAGA GCACAGAGGT CCTGAGCCCC
    CCAGCTCCAC AGGGAGGAGG GCTTAGGTGG GGGGTACCCT CCACCCTGCC CCAGAGCTTT
    GCCAGAGTGA CTACCTCCAT GACTGTGGCC CTCCACCCAC CCCCACTGCC TGGTGCCTAC
    ATCCACCCAG CCTCTGAGGA GCCTACTTGG TCACCTGCTG CCACACCAGC TGCCAATGGC
    CCCAGCAACC TCGGGTCTAG GGGACTGTGT CCAGCCACGG GGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006052641.2
    CDS429..4073
    Translation

    Target ORF information:

    RefSeq Version XM_006052579.2
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis patched 2 (PTCH2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006052579.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCTCGGC CGCCACCTCT CGGGGAGCTG CCCCCGGGCT ACACACCCCC AGCTCGACCT 
    GCAACACCCC AGATCCTAGC TGGGAGCCTG AAGGCTCCGC TCTGGCTCCG TGCTTACTTC
    CAGGGCCTGC TCTTCTCTTT GGGCTGCGGG ATCCAGAGAC ACTGTGGCAA AGTGCTCTTC
    CTGGGACTGT TGGCCTTTGG GGCCCTAGCA CTGGGTCTCC GCGTGGCCAT CATTGAGACA
    GACCTAGAAC AGCTCTGGGT GGAAGTGGGC AGCCGGGTGA GCCAGGAACT GGAATACACC
    AAGGAGAAGC TGGGGGAGGA GGCTGCGTAT ACCTCCCAGA TGTTGATACA GACCCCGCGT
    CAGGAGGGGG AGAACGTCCT CACGCCCGAG GCACTTGACC TCCACCTCCA GGCAGCCCTC
    ACCGCCAGTA AAGTGCAAGT ATCCCTCTAT GGAAAGTCCT GGGATCTGAA CAAAATCTGC
    TACAAGTCAG GAGTTCCCCT AATTGAAAAT GGAATGATTG AGCGGATGAT TGAAAAGCTG
    TTTCCCTGCG TGATCCTCAC CCCCCTCGAC TGCTTCTGGG AGGGAGCCAA ACTCCAAGGG
    GGCTCTGCCT ACTTGCCGGG CCGCCCAGAC ATCCAGTGGA CCAACCTGGA CCCAGAGCAG
    CTGCTTGAGG AGCTGGGCCC CTTTGCCTCC CTTGAGGGCT TCCGGGAGCT GCTAGACAAG
    GCACAGGTGG GCCAGGCCTA CGTGGGTCGG CCCTGTCTGC ACCCTGACGA CCTCCACTGC
    CCACCTAGTG CCCCTAACCA TCACAGCAAG CAGGCTCCCA ACGTGGCTCA GGAGTTGAGT
    GGGGGCTGCC ATGGCTTCTC CCACAAGTTT ATGCACTGGC AGGAGGAGCT GCTGCTGGGA
    GGCATGGCCA GAGACCCCCA AGGACAGCTG CTGAGGGCAG AGGCCCTGCA GAGCACCTTC
    CTGCTAATGA GCCCCCGCCA GCTCTACGAG CACTTCCGAG GTGACTACCA GACGCATGAC
    ATCGGCTGGA GCGAGGAGCA GGCCGGCACG GTCCTTCAGG CCTGGCAGCG ACGCTTTGTG
    CAGCTGGCCC AGGAGGCCCT GCCCGAGAAC GCATCCCAGC AGATTCACGC CTTCTCCTCT
    ACCACCCTGG ATGACATCCT GCACGCCTTC TCTGAAGTCA GCGCTGCTCG CGTGGCGGGG
    GGCTACCTGC TCATGCTAGC CTATGCCTGT GTGACGATGC TGCGGTGGGA CTGTGCTCAG
    TCCCAGGGTG CAGTGGGCCT CGCTGGTGTG CTGCTGGTGG CCCTGGCAGT GGCCTCGGGC
    CTGGGGCTCT GTGCCCTGTT GGGCATCGCC TTCAACGCTG CCACTACCCA GGTGCTGCCT
    TTCTTGGCAC TGGGCATCGG CGTGGATGAC ATATTCCTGC TGGCACACGC CTTCACAGAG
    GCTCCACCTG GCACCCCTCT CCAGGAGCGC ACGGGGGAGT GTCTGCGGCG CACAGGCACC
    GGTGTCACAC TCACATCCGT CAACAATATG GTCGCCTTCT TCATGGCCGC CCTAGTCCCC
    ATCCCTGCAC TTCGGGCCTT CTCCTTGCAG GCAGCCATAG TGGTGGGCTG CAACTTTGCA
    GCCGTGATGC TTGTCTTCCC AGCGGTCCTC AGCCTGGACC TGCAGCGGCG CCACTGCCGG
    CGCCTGGATG TGCTCTGCTG CTTCTCTAGC CCCTGCTCTG CTCAGGTGAT TCAGATTCTG
    CCCCAGGAGC TGGGGAACGG GACGGTACCA GTGGGCGTTG CCCACCTGAC TGCCACGGTT
    CAAGCCTTTG CCCACTGTGA AGCCAGGAGC CAACATGTGG TCACCATCTT GCCTCCCCAA
    GCTCACCTGG TGCCGCCACC TTCTGACCCT TTGGGCTCTG AGCTCTTCAG CCCAGGAGGG
    TCCACACGGG ACCTTCTAGG GCAGGAGGAG GGGACAGGGC AGAAGGCAGC CTGCAAGTCC
    CTGCCCTGTG CCCGCTGGAA TCTTGCCCAT TTCGCCCGCT CTCAGTTTGC ACCCTTGTTG
    CTCCAGTCCC CCACCAAGCT TAGTCACCTT TCCCCACAGG CTGTAGTACT GGTACTTTTT
    GGGGCTCTCC TGGGCCTGAG CCTCTATGGA GCGACCTTGG TGCAGGACGG TCTGGCCCTG
    ACTGATGTGG TGCCGCGTGG CACCAAGGAG CATGCCTTCC TGAGCGCCCA GCTCAGGTAC
    TTCTCTCTGT ACGAGGTGGC TCTGGTGACA CAGGGTGGCT TTGACTACGC CCACTCCCAA
    CGCGCCCTCT TTGATCTGCA CCAGCGCTTC AGTTCCCTCA AGGCCGTGCT GCCCCCACCG
    GCCACCCAGG CACCCCGCAC CTGGCTGCAC TACTACCGCA GCTGGCTACA GGGAATCCAG
    GCTGCGTTTG ACCAGGACTG GGCTTCTGGG CGCATCACCC GCCACTCATA CCGCAATGGC
    TCTGAGGATG GGGCCCTGGC ATACAAGCTG CTTGTCCAGA CCGGGGATGC CCAGGAGCCT
    CTAGATTTCA GCCAGCTGAC CACCAAGAAG CTGGTGGACA AGGAAGGGCT GATCCCACCC
    GAGCTCTTCT ACATGGGGCT GACCGTGTGG GTGAGCAGTG ACCCCCTGGG TCTGGCAGCC
    TCACAGGCCA ACTTCTATCC CCCACCTCCT GAGTGGCTGC ATGACAAGTA CGATACCACG
    GGGGAGAATC TTCGCATCCC GGCGGCCCAG CCCTTGGAGT TTGCCCAGTT CCCCTTCCTC
    CTGCGCGGCC TCCAGAAGAC TGCAGACTTC GTGGAGGCCA TCGAGGGGGC CCGGGCAGCG
    TGTGCCGAGG CCGGCCAGGC CGGGGTGCAT GCCTACCCCA GCGGCTCCCC CTTCCTCTTC
    TGGGAGCAGT ACCTGAGCCT GCGGCGCTGC TTCCTGCTAG CGGTCTGCAT CCTGCTGCTG
    TGTACTTTCC TCGTCTGTGC CCTGCTGCTG CTCAACCCCT GGACGGCTGT GCTCATAGTA
    CTGGTCCTGG CGATGATGAC TGTGGAGCTC TTTGGCATCA TGGGTTTCCT GGGCATCAAG
    CTGAGTGCCA TCCCGGTGGT GATTCTTGTG GCCTCCATAG GCATCGGTGT CGAGTTCACT
    GTCCACGTGG CTCTGGGCTT CCTGACCGCT CAGGGTAGCC GGAACCTGCG GGCTGCCCGG
    GCCCTGGAGC GCACATTTGC CCCAGTGACC GATGGGGCCA TCTCCACGTT GCTAGGTCTG
    CTCATGCTTG CTGGCTCCAA CTTTGACTTC ATCGTAAGGT ACTTCTTTGT GGTGCTGACA
    ATACTTACAC TCCTGGGCCT CCTCCACGGG CTCGTGCTGC TGCCGGTACT GCTGTCCATC
    CTGGGCCCCC CACCAGAGGT GGTACAGATG TACAAGGAGA GCACAGAGGT CCTGAGCCCC
    CCAGCTCCAC AGGGAGGAGG GCTTAGGTGG GGGGTACCCT CCACCCTGCC CCAGAGCTTT
    GCCAGAGTGA CTACCTCCAT GACTGTGGCC CTCCACCCAC CCCCACTGCC TGGTGCCTAC
    ATCCACCCAG CCTCTGAGGA GCCTACTTGG TCACCTGCTG CCACACCAGC TGCCAATGGC
    CCCAGCAACC TCGGGTCTAG GGGACTGTGT CCAGCCACGG GGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu68867
    Clone ID Related Accession (Same CDS sequence) XM_006052580.2
    Accession Version XM_006052580.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3624bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product protein patched homolog 2 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037550.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006052580.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCTCGGC CGCCACCTCT CGGGGAGCTG CCCCCGGGCT ACACACCCCC AGCTCGACCT 
    GCAACACCCC AGATCCTAGC TGGGAGCCTG AAGGCTCCGC TCTGGCTCCG TGCTTACTTC
    CAGGGCCTGC TCTTCTCTTT GGGCTGCGGG ATCCAGAGAC ACTGTGGCAA AGTGCTCTTC
    CTGGGACTGT TGGCCTTTGG GGCCCTAGCA CTGGGTCTCC GCGTGGCCAT CATTGAGACA
    GACCTAGAAC AGCTCTGGGT GGAAGTGGGC AGCCGGGTGA GCCAGGAACT GGAATACACC
    AAGGAGAAGC TGGGGGAGGA GGCTGCGTAT ACCTCCCAGA TGTTGATACA GACCCCGCGT
    CAGGAGGGGG AGAACGTCCT CACGCCCGAG GCACTTGACC TCCACCTCCA GGCAGCCCTC
    ACCGCCAGTA AAGTGCAAGT ATCCCTCTAT GGAAAGTCCT GGGATCTGAA CAAAATCTGC
    TACAAGTCAG GAGTTCCCCT AATTGAAAAT GGAATGATTG AGCGGATGAT TGAAAAGCTG
    TTTCCCTGCG TGATCCTCAC CCCCCTCGAC TGCTTCTGGG AGGGAGCCAA ACTCCAAGGG
    GGCTCTGCCT ACTTGCCGGG CCGCCCAGAC ATCCAGTGGA CCAACCTGGA CCCAGAGCAG
    CTGCTTGAGG AGCTGGGCCC CTTTGCCTCC CTTGAGGGCT TCCGGGAGCT GCTAGACAAG
    GCACAGGTGG GCCAGGCCTA CGTGGGTCGG CCCTGTCTGC ACCCTGACGA CCTCCACTGC
    CCACCTAGTG CCCCTAACCA TCACAGCAAG CAGGCTCCCA ACGTGGCTCA GGAGTTGAGT
    GGGGGCTGCC ATGGCTTCTC CCACAAGTTT ATGCACTGGC AGGAGGAGCT GCTGCTGGGA
    GGCATGGCCA GAGACCCCCA AGGACAGCTG CTGAGGGCAG AGGCCCTGCA GAGCACCTTC
    CTGCTAATGA GCCCCCGCCA GCTCTACGAG CACTTCCGAG GTGACTACCA GACGCATGAC
    ATCGGCTGGA GCGAGGAGCA GGCCGGCACG GTCCTTCAGG CCTGGCAGCG ACGCTTTGTG
    CAGCTGGCCC AGGAGGCCCT GCCCGAGAAC GCATCCCAGC AGATTCACGC CTTCTCCTCT
    ACCACCCTGG ATGACATCCT GCACGCCTTC TCTGAAGTCA GCGCTGCTCG CGTGGCGGGG
    GGCTACCTGC TCATGCTAGC CTATGCCTGT GTGACGATGC TGCGGTGGGA CTGTGCTCAG
    TCCCAGGGTG CAGTGGGCCT CGCTGGTGTG CTGCTGGTGG CCCTGGCAGT GGCCTCGGGC
    CTGGGGCTCT GTGCCCTGTT GGGCATCGCC TTCAACGCTG CCACTACCCA GGTGCTGCCT
    TTCTTGGCAC TGGGCATCGG CGTGGATGAC ATATTCCTGC TGGCACACGC CTTCACAGAG
    GCTCCACCTG GCACCCCTCT CCAGGAGCGC ACGGGGGAGT GTCTGCGGCG CACAGGCACC
    GGTGTCACAC TCACATCCGT CAACAATATG GTCGCCTTCT TCATGGCCGC CCTAGTCCCC
    ATCCCTGCAC TTCGGGCCTT CTCCTTGCAG GCAGCCATAG TGGTGGGCTG CAACTTTGCA
    GCCGTGATGC TTGTCTTCCC AGCGGTCCTC AGCCTGGACC TGCAGCGGCG CCACTGCCGG
    CGCCTGGATG TGCTCTGCTG CTTCTCTAGC CCCTGCTCTG CTCAGGTGAT TCAGATTCTG
    CCCCAGGAGC TGGGGAACGG GACGGTACCA GTGGGCGTTG CCCACCTGAC TGCCACGGTT
    CAAGCCTTTG CCCACTGTGA AGCCAGGAGC CAACATGTGG TCACCATCTT GCCTCCCCAA
    GCTCACCTGG TGCCGCCACC TTCTGACCCT TTGGGCTCTG AGCTCTTCAG CCCAGGAGGG
    TCCACACGGG ACCTTCTAGG GCAGGAGGAG GGGACAGGGC AGAAGGCAGC CTGCAAGTCC
    CTGCCCTGTG CCCGCTGGAA TCTTGCCCAT TTCGCCCGCT CTCAGTTTGC ACCCTTGTTG
    CTCCAGTCCC CCACCAAGGC TGTAGTACTG GTACTTTTTG GGGCTCTCCT GGGCCTGAGC
    CTCTATGGAG CGACCTTGGT GCAGGACGGT CTGGCCCTGA CTGATGTGGT GCCGCGTGGC
    ACCAAGGAGC ATGCCTTCCT GAGCGCCCAG CTCAGGTACT TCTCTCTGTA CGAGGTGGCT
    CTGGTGACAC AGGGTGGCTT TGACTACGCC CACTCCCAAC GCGCCCTCTT TGATCTGCAC
    CAGCGCTTCA GTTCCCTCAA GGCCGTGCTG CCCCCACCGG CCACCCAGGC ACCCCGCACC
    TGGCTGCACT ACTACCGCAG CTGGCTACAG GGAATCCAGG CTGCGTTTGA CCAGGACTGG
    GCTTCTGGGC GCATCACCCG CCACTCATAC CGCAATGGCT CTGAGGATGG GGCCCTGGCA
    TACAAGCTGC TTGTCCAGAC CGGGGATGCC CAGGAGCCTC TAGATTTCAG CCAGCTGACC
    ACCAAGAAGC TGGTGGACAA GGAAGGGCTG ATCCCACCCG AGCTCTTCTA CATGGGGCTG
    ACCGTGTGGG TGAGCAGTGA CCCCCTGGGT CTGGCAGCCT CACAGGCCAA CTTCTATCCC
    CCACCTCCTG AGTGGCTGCA TGACAAGTAC GATACCACGG GGGAGAATCT TCGCATCCCG
    GCGGCCCAGC CCTTGGAGTT TGCCCAGTTC CCCTTCCTCC TGCGCGGCCT CCAGAAGACT
    GCAGACTTCG TGGAGGCCAT CGAGGGGGCC CGGGCAGCGT GTGCCGAGGC CGGCCAGGCC
    GGGGTGCATG CCTACCCCAG CGGCTCCCCC TTCCTCTTCT GGGAGCAGTA CCTGAGCCTG
    CGGCGCTGCT TCCTGCTAGC GGTCTGCATC CTGCTGCTGT GTACTTTCCT CGTCTGTGCC
    CTGCTGCTGC TCAACCCCTG GACGGCTGTG CTCATAGTAC TGGTCCTGGC GATGATGACT
    GTGGAGCTCT TTGGCATCAT GGGTTTCCTG GGCATCAAGC TGAGTGCCAT CCCGGTGGTG
    ATTCTTGTGG CCTCCATAGG CATCGGTGTC GAGTTCACTG TCCACGTGGC TCTGGGCTTC
    CTGACCGCTC AGGGTAGCCG GAACCTGCGG GCTGCCCGGG CCCTGGAGCG CACATTTGCC
    CCAGTGACCG ATGGGGCCAT CTCCACGTTG CTAGGTCTGC TCATGCTTGC TGGCTCCAAC
    TTTGACTTCA TCGTAAGGTA CTTCTTTGTG GTGCTGACAA TACTTACACT CCTGGGCCTC
    CTCCACGGGC TCGTGCTGCT GCCGGTACTG CTGTCCATCC TGGGCCCCCC ACCAGAGGTG
    GTACAGATGT ACAAGGAGAG CACAGAGGTC CTGAGCCCCC CAGCTCCACA GGGAGGAGGG
    CTTAGGTGGG GGGTACCCTC CACCCTGCCC CAGAGCTTTG CCAGAGTGAC TACCTCCATG
    ACTGTGGCCC TCCACCCACC CCCACTGCCT GGTGCCTACA TCCACCCAGC CTCTGAGGAG
    CCTACTTGGT CACCTGCTGC CACACCAGCT GCCAATGGCC CCAGCAACCT CGGGTCTAGG
    GGACTGTGTC CAGCCACGGG GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006052642.2
    CDS1..3624
    Translation

    Target ORF information:

    RefSeq Version XM_006052580.2
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis patched 2 (PTCH2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006052580.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCTCGGC CGCCACCTCT CGGGGAGCTG CCCCCGGGCT ACACACCCCC AGCTCGACCT 
    GCAACACCCC AGATCCTAGC TGGGAGCCTG AAGGCTCCGC TCTGGCTCCG TGCTTACTTC
    CAGGGCCTGC TCTTCTCTTT GGGCTGCGGG ATCCAGAGAC ACTGTGGCAA AGTGCTCTTC
    CTGGGACTGT TGGCCTTTGG GGCCCTAGCA CTGGGTCTCC GCGTGGCCAT CATTGAGACA
    GACCTAGAAC AGCTCTGGGT GGAAGTGGGC AGCCGGGTGA GCCAGGAACT GGAATACACC
    AAGGAGAAGC TGGGGGAGGA GGCTGCGTAT ACCTCCCAGA TGTTGATACA GACCCCGCGT
    CAGGAGGGGG AGAACGTCCT CACGCCCGAG GCACTTGACC TCCACCTCCA GGCAGCCCTC
    ACCGCCAGTA AAGTGCAAGT ATCCCTCTAT GGAAAGTCCT GGGATCTGAA CAAAATCTGC
    TACAAGTCAG GAGTTCCCCT AATTGAAAAT GGAATGATTG AGCGGATGAT TGAAAAGCTG
    TTTCCCTGCG TGATCCTCAC CCCCCTCGAC TGCTTCTGGG AGGGAGCCAA ACTCCAAGGG
    GGCTCTGCCT ACTTGCCGGG CCGCCCAGAC ATCCAGTGGA CCAACCTGGA CCCAGAGCAG
    CTGCTTGAGG AGCTGGGCCC CTTTGCCTCC CTTGAGGGCT TCCGGGAGCT GCTAGACAAG
    GCACAGGTGG GCCAGGCCTA CGTGGGTCGG CCCTGTCTGC ACCCTGACGA CCTCCACTGC
    CCACCTAGTG CCCCTAACCA TCACAGCAAG CAGGCTCCCA ACGTGGCTCA GGAGTTGAGT
    GGGGGCTGCC ATGGCTTCTC CCACAAGTTT ATGCACTGGC AGGAGGAGCT GCTGCTGGGA
    GGCATGGCCA GAGACCCCCA AGGACAGCTG CTGAGGGCAG AGGCCCTGCA GAGCACCTTC
    CTGCTAATGA GCCCCCGCCA GCTCTACGAG CACTTCCGAG GTGACTACCA GACGCATGAC
    ATCGGCTGGA GCGAGGAGCA GGCCGGCACG GTCCTTCAGG CCTGGCAGCG ACGCTTTGTG
    CAGCTGGCCC AGGAGGCCCT GCCCGAGAAC GCATCCCAGC AGATTCACGC CTTCTCCTCT
    ACCACCCTGG ATGACATCCT GCACGCCTTC TCTGAAGTCA GCGCTGCTCG CGTGGCGGGG
    GGCTACCTGC TCATGCTAGC CTATGCCTGT GTGACGATGC TGCGGTGGGA CTGTGCTCAG
    TCCCAGGGTG CAGTGGGCCT CGCTGGTGTG CTGCTGGTGG CCCTGGCAGT GGCCTCGGGC
    CTGGGGCTCT GTGCCCTGTT GGGCATCGCC TTCAACGCTG CCACTACCCA GGTGCTGCCT
    TTCTTGGCAC TGGGCATCGG CGTGGATGAC ATATTCCTGC TGGCACACGC CTTCACAGAG
    GCTCCACCTG GCACCCCTCT CCAGGAGCGC ACGGGGGAGT GTCTGCGGCG CACAGGCACC
    GGTGTCACAC TCACATCCGT CAACAATATG GTCGCCTTCT TCATGGCCGC CCTAGTCCCC
    ATCCCTGCAC TTCGGGCCTT CTCCTTGCAG GCAGCCATAG TGGTGGGCTG CAACTTTGCA
    GCCGTGATGC TTGTCTTCCC AGCGGTCCTC AGCCTGGACC TGCAGCGGCG CCACTGCCGG
    CGCCTGGATG TGCTCTGCTG CTTCTCTAGC CCCTGCTCTG CTCAGGTGAT TCAGATTCTG
    CCCCAGGAGC TGGGGAACGG GACGGTACCA GTGGGCGTTG CCCACCTGAC TGCCACGGTT
    CAAGCCTTTG CCCACTGTGA AGCCAGGAGC CAACATGTGG TCACCATCTT GCCTCCCCAA
    GCTCACCTGG TGCCGCCACC TTCTGACCCT TTGGGCTCTG AGCTCTTCAG CCCAGGAGGG
    TCCACACGGG ACCTTCTAGG GCAGGAGGAG GGGACAGGGC AGAAGGCAGC CTGCAAGTCC
    CTGCCCTGTG CCCGCTGGAA TCTTGCCCAT TTCGCCCGCT CTCAGTTTGC ACCCTTGTTG
    CTCCAGTCCC CCACCAAGGC TGTAGTACTG GTACTTTTTG GGGCTCTCCT GGGCCTGAGC
    CTCTATGGAG CGACCTTGGT GCAGGACGGT CTGGCCCTGA CTGATGTGGT GCCGCGTGGC
    ACCAAGGAGC ATGCCTTCCT GAGCGCCCAG CTCAGGTACT TCTCTCTGTA CGAGGTGGCT
    CTGGTGACAC AGGGTGGCTT TGACTACGCC CACTCCCAAC GCGCCCTCTT TGATCTGCAC
    CAGCGCTTCA GTTCCCTCAA GGCCGTGCTG CCCCCACCGG CCACCCAGGC ACCCCGCACC
    TGGCTGCACT ACTACCGCAG CTGGCTACAG GGAATCCAGG CTGCGTTTGA CCAGGACTGG
    GCTTCTGGGC GCATCACCCG CCACTCATAC CGCAATGGCT CTGAGGATGG GGCCCTGGCA
    TACAAGCTGC TTGTCCAGAC CGGGGATGCC CAGGAGCCTC TAGATTTCAG CCAGCTGACC
    ACCAAGAAGC TGGTGGACAA GGAAGGGCTG ATCCCACCCG AGCTCTTCTA CATGGGGCTG
    ACCGTGTGGG TGAGCAGTGA CCCCCTGGGT CTGGCAGCCT CACAGGCCAA CTTCTATCCC
    CCACCTCCTG AGTGGCTGCA TGACAAGTAC GATACCACGG GGGAGAATCT TCGCATCCCG
    GCGGCCCAGC CCTTGGAGTT TGCCCAGTTC CCCTTCCTCC TGCGCGGCCT CCAGAAGACT
    GCAGACTTCG TGGAGGCCAT CGAGGGGGCC CGGGCAGCGT GTGCCGAGGC CGGCCAGGCC
    GGGGTGCATG CCTACCCCAG CGGCTCCCCC TTCCTCTTCT GGGAGCAGTA CCTGAGCCTG
    CGGCGCTGCT TCCTGCTAGC GGTCTGCATC CTGCTGCTGT GTACTTTCCT CGTCTGTGCC
    CTGCTGCTGC TCAACCCCTG GACGGCTGTG CTCATAGTAC TGGTCCTGGC GATGATGACT
    GTGGAGCTCT TTGGCATCAT GGGTTTCCTG GGCATCAAGC TGAGTGCCAT CCCGGTGGTG
    ATTCTTGTGG CCTCCATAGG CATCGGTGTC GAGTTCACTG TCCACGTGGC TCTGGGCTTC
    CTGACCGCTC AGGGTAGCCG GAACCTGCGG GCTGCCCGGG CCCTGGAGCG CACATTTGCC
    CCAGTGACCG ATGGGGCCAT CTCCACGTTG CTAGGTCTGC TCATGCTTGC TGGCTCCAAC
    TTTGACTTCA TCGTAAGGTA CTTCTTTGTG GTGCTGACAA TACTTACACT CCTGGGCCTC
    CTCCACGGGC TCGTGCTGCT GCCGGTACTG CTGTCCATCC TGGGCCCCCC ACCAGAGGTG
    GTACAGATGT ACAAGGAGAG CACAGAGGTC CTGAGCCCCC CAGCTCCACA GGGAGGAGGG
    CTTAGGTGGG GGGTACCCTC CACCCTGCCC CAGAGCTTTG CCAGAGTGAC TACCTCCATG
    ACTGTGGCCC TCCACCCACC CCCACTGCCT GGTGCCTACA TCCACCCAGC CTCTGAGGAG
    CCTACTTGGT CACCTGCTGC CACACCAGCT GCCAATGGCC CCAGCAACCT CGGGTCTAGG
    GGACTGTGTC CAGCCACGGG GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.