PPFIA4 cDNA ORF clone, Bubalus bubalis(water buffalo)

The following PPFIA4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PPFIA4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBu41789 XM_025277803.1
Latest version!
Bubalus bubalis PTPRF interacting protein alpha 4 (PPFIA4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBu41790 XM_025277804.1
Latest version!
Bubalus bubalis PTPRF interacting protein alpha 4 (PPFIA4), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBu41791 XM_025277805.1
Latest version!
Bubalus bubalis PTPRF interacting protein alpha 4 (PPFIA4), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBu41792 XM_025277806.1
Latest version!
Bubalus bubalis PTPRF interacting protein alpha 4 (PPFIA4), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBu41793 XM_025277807.1
Latest version!
Bubalus bubalis PTPRF interacting protein alpha 4 (PPFIA4), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBu41794 XM_025277810.1
Latest version!
Bubalus bubalis PTPRF interacting protein alpha 4 (PPFIA4), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBu41794 XM_025277808.1
Latest version!
Bubalus bubalis PTPRF interacting protein alpha 4 (PPFIA4), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBu41794 XM_025277809.1
Latest version!
Bubalus bubalis PTPRF interacting protein alpha 4 (PPFIA4), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBu41794 XM_025277811.1
Latest version!
Bubalus bubalis PTPRF interacting protein alpha 4 (PPFIA4), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBu41795 XM_025277812.1
Latest version!
Bubalus bubalis PTPRF interacting protein alpha 4 (PPFIA4), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBu41796 XM_025277813.1
Latest version!
Bubalus bubalis PTPRF interacting protein alpha 4 (PPFIA4), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBu41797 XM_025277814.1
Latest version!
Bubalus bubalis PTPRF interacting protein alpha 4 (PPFIA4), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBu41798 XM_025277815.1
Latest version!
Bubalus bubalis PTPRF interacting protein alpha 4 (PPFIA4), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBu41799 XM_025277817.1
Latest version!
Bubalus bubalis PTPRF interacting protein alpha 4 (PPFIA4), transcript variant X16, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBu41799 XM_025277816.1
Latest version!
Bubalus bubalis PTPRF interacting protein alpha 4 (PPFIA4), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBu26664 XM_006072413.1
Latest version!
Bubalus bubalis protein tyrosine phosphatase, receptor type, f polypeptide (PTPRF), interacting protein (liprin), alpha 4 (PPFIA4), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBu41789
    Clone ID Related Accession (Same CDS sequence) XM_025277803.1
    Accession Version XM_025277803.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3894bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product liprin-alpha-4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_020228958.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGAGGCAG GGCTCCCTTG GGCCCTAAAA CTTGACTGGC GTTGGGTAAA AATCTGGCTC 
    CCCGCCACCT CACGAGATAC TCTCCCGGCC GAGCCAAGAT GGCGCGCAGC CCCAAGTGGC
    TCCACGTCAC GAGGGAGGGA GCGCCGGGGC GGGGGCGTGG CCTCGAGGGG GCCGGCTAGC
    CCCGCCCTTG AGGTGGGGCC GGGGGCCGTC CGCAGAAGGC GAGGGCGGGG CGCTTCGGCT
    CGGGAAGGCT GCGGCTGCCT CGGCGCCGGC TCCGCTCGCG AGGACAAGTC CGGCGCCTCC
    AGGCCGGGCC GGCTTCCTGC GCAGAGCCAA GCTGCCCGGC CTTCGGAGTT TACCACCTTA
    ACCCGGGAGC TGAGTATGTG TCGGGAGCAG CTTCTAGAGA GGGAGGAAGA GATATCAGAG
    CTGAAAGCAG AGCGGAATAA CACGAGGTTG CTTCTGGAAC ATCTGGAGTG CCTGGTGTCC
    CGCCATGAGC GGTCACTGAG GATGACTGTG GTGAAGCGTC AGGCCCAGTC ACCCTCAGGG
    GTCTCCAGTG AGGTGGAGGT GCTGAAGGCT CTCAAGTCAC TGTTTGAGCA CCACAAGGCC
    CTGGATGAGA AGGTGCGAGA GCGGCTCCGG GTGGCCCTCG AGAGAGTGAC CACTTTGGAG
    GAACAGCTGG CGTGTGCCCA CCAGCAGGTG TCTTCCCTAC AGCAGGGGTC AGGGGTTCGG
    GATGGAGTGG CCGAAGAGGA GGGGACTGTG GAGCTGGGAT CAAAAGGCCT GTGGAAGGAG
    GACACAGGCC AGGTTGAGGA GCTGCAGGAG CTTCTGGAGA AGCAGAACTT TGAGTTGAGC
    CAGGCCCGGG AGCGACTGGT CACCCTGACA GCAACTGTGG CTGAACTGGA GGAGGACCTG
    GGCACAGCCC GCCGGGACCT CATCAAGTCG GAGGAACTGA GCAGCAAACA CCAGCGGGAC
    CTCCGGGAAG CTCTAGCCCA GAAAGAGGAC ATGGAGGAGC GGATCACCAC CCTGGAGAAG
    CGCTACCTGG CTGCTCAGCG TGAGGCTACA TCAATACATG ACCTCAATGA CAAACTGGAG
    AACGAGCTAG CCAATAGGGA ATCCTTGCAT CGCCAGTGTG AGGAGAAAGC CCGATATCTG
    CAGGAGCTAC TGGAGGTAGC CGAGCAGAAA CTTCAGCAGA CAATGCGCAA GGCCGAGACG
    CTGCCAGAGG TGGAGGCTGA GCTGGCCCAA AGAGTTGCGG CCCTCACCAA GGCTGAAGAG
    CGACATGGCA ACACTGAGGA GCACCTCCGA CAGCTGGAGG GACAGCTGGA GGAGAAGAAC
    CAAGAGCTGG CGCGGGTGCG CCAGCGGGAG AGGATGAATG AAGCCCACAA CAAGCGCCTG
    TCAGACACTG TGGACCGGCT GCTTATCGAG GCGAAGGAGC GCCTGCAGCT CCACCTCAGG
    GAGCGCATGG TGGCCCTGGA GGAGAAGAAC ACATTGATCC AAGAGCTGGA GACCTCCCAG
    CGGCAGATTG AAGAGCAGCA CCATCACAAG GGCCGCCTGT CTGAAGAGAT TGAGAAACTG
    CGCCAAGAGG TGGACCAACT GAAGGGTCGA GGAGGACCAT TTGTGGATGG CATCCACTCC
    AGGGCGTACA CGGGCAGTGC AATGGATGTG CGGTTCTCCC TGAGCACAAC TGCCCACACA
    TCTCAAGGTC TGCGTCGTTG TTACTCAGCA CGGCGGGAAC CGCCTGCCAA GGACTGGGAG
    CCATCTCCAC GGCCTGCGGT GCTGGTCCCG ACAACCACCC CTGCCTTTGA CAGTGACCGT
    GAGATCTCTG ATGTGGATGA GGATGAACCA GGGGGTCTGG TGGGCTCCAT GGACGTTGTC
    TCCCCCAGCA GCCACTCAGA CGCCCAGACC CTGGCCATGA TGCTGCAGGA GCAGCTGGAT
    GCTATCAACG AGGAGATCAG GATGATCCAG GAAGAGAAGG AGTCCACAGA GCTCCGTGCT
    GAGGAGCTGG AAACTCGAGT GACTAGTGGC AGCATGGAGG CCCTAGACCT GACCCAGCTG
    CACAAACGCG GTTCCATCCC CACCTCTCTG ACCGCCCTGT CCCTGGCCAG CGCGTCCCCT
    CCACTTAGCG GCCGTGCCAC ACCTAAGCTC ACCTCCCGCA GTGCTGCCCA GGACCTGGAT
    CGGATGGGGG TCATGACCTT GCCCAGTGAC TTAAGAAAGC ATAGGAGGAA GCTGCTGTCA
    CCAGTGTCTT GGGAAGAGAA CCGAGAGGAT AAAGCCACCA TAAAATGTGA GACTTCCCCT
    CCTTCCTCAC CCAGGACACT GCAGCTAGAG AAGCTTGGCC TCCCTGCCCT GAGCCAGGAA
    GAAGGCAAGA GTGCTTTGGA GGATCCAGGC AGCAACCCCA GCAGCAGCAA CAGCAGCCAG
    GACTCCTTGC ACAAGGGTGC CAAGCGCAAG GGCATCAAAT CATCCATCGG CCGCCTGTTT
    GGGAAGAAGG AGAAAGGCAG GCTGATCCAG CCGAGCCGGG ATGGACCCAC AGGCCATGTT
    GTACTACTAA CAGACTCTGA GCTCGGTCTG CAGGAGCCCA TGGTGCCTGG CAAGCTGGGA
    ACCCAGGCAG AAAAGGACCG GAGGCTGAAG AAGAAACACC AGCTGCTTGA AGATGCCCGG
    AGAAAAGGAA TGCCCTTTGC CCAGTGGGAC GGCCCTACTG TGGTCTCCTG GCTAGAGCTC
    TGGGTGGGGA TGCCTGCTTG GTATGTGGCA GCCTGCCGGG CCAACGTCAA GAGTGGCGCC
    ATCATGTCAG CCCTATCGGA CACAGAGATC CAGCGAGAAA TTGGCATCAG CAATGCCCTG
    CACCGGCTCA AGCTCCGGCT GGCCATCCAG GAGATGGTGT CGCTGACCAG CCCCTCTGCC
    CCGCCCACCT CCAGGACTTC TTCTGGGAAT GTCTGGGTCA CCCACGAAGA GATGGAAACT
    CTGGCAACGT CCACTAAAAC AGACAGTGAG GAGGGCAGCT GGGCTCAGAC CCTGGTCTAT
    GGGGATATGA ACCACGAGTG GATTGGGAAC CAGTGGCTCC CCAGCCTGGG GCTCCCCCAG
    TACCGCAGCT ACTTCATGGA GTGCCTCGTG GACGCTCGCA TGCTGGATCA CCTCACCAAG
    AAGGACTTGC GGGTCCACCT GAAGATGGTG GACAGCTCCC ACCGAACCAG TCTTCAGTAT
    GGCATCATGT GCCTGAAGAG GCTGAATTAT GACCGGAAGG AGCTGGAGAA GCGGCGGGAG
    GAGAGCCAGC ATGAGATCAA GGATGTGCTG GTCTGGACCA ATGACCAGGT AGTTCATTGG
    GTCCAGTCTA TTGGGCTCCG AGACTACGCA GGAAACCTCC ATGAGAGTGG TGTGCATGGC
    GCTTTGCTGG CCCTGGATGA GAACTTTGAC CACAACACAC TGGCCCTGGT TCTCCAGATA
    CCCACACAAA ATACCCAGGC ACGCCAAGTG TTGGAGAGAG AGTTCAACAA CCTGTTGGCT
    TTGGGCACAG ACCGGAAGCT GGATGATGGG GAAGACAAGG CGCTACGCCG CGCGCCCTCC
    TGGAGGAAAC GCTTCCGCCC GCGGGACCCC CACGGCCGCG CCTCGCTCAG CGCCGCGGCC
    GACACGCTCC CGGCAACCTT CCGCGCGTCC ACCGTGGGGC CCGTGCCGCC TCCAGCATCC
    CCGCGGAAGA AGATCACGCC TGAAGGTCTG GACCGCTCAC TCCACCGGAG ACCAGGGTGC
    AGAACCAAGG GCTGGGGTGG CCCTTCCAAA GAAAGGGCGC TTCAGACTTC CTTGCTTGGG
    TTATTGGGCC TTCTGGTTTC TGTCCGTCTC TTGGTTGCAC TAGATGGTCT ACGTCTGCTT
    TTGGCATCCC CCCTCCCGCC ATTTTCTCTC CCCCAACTCA TCCGCACCTT GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025133588.1
    CDS563..4456
    Translation

    Target ORF information:

    RefSeq Version XM_025277803.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis PTPRF interacting protein alpha 4 (PPFIA4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025277803.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGAGGCAG GGCTCCCTTG GGCCCTAAAA CTTGACTGGC GTTGGGTAAA AATCTGGCTC 
    CCCGCCACCT CACGAGATAC TCTCCCGGCC GAGCCAAGAT GGCGCGCAGC CCCAAGTGGC
    TCCACGTCAC GAGGGAGGGA GCGCCGGGGC GGGGGCGTGG CCTCGAGGGG GCCGGCTAGC
    CCCGCCCTTG AGGTGGGGCC GGGGGCCGTC CGCAGAAGGC GAGGGCGGGG CGCTTCGGCT
    CGGGAAGGCT GCGGCTGCCT CGGCGCCGGC TCCGCTCGCG AGGACAAGTC CGGCGCCTCC
    AGGCCGGGCC GGCTTCCTGC GCAGAGCCAA GCTGCCCGGC CTTCGGAGTT TACCACCTTA
    ACCCGGGAGC TGAGTATGTG TCGGGAGCAG CTTCTAGAGA GGGAGGAAGA GATATCAGAG
    CTGAAAGCAG AGCGGAATAA CACGAGGTTG CTTCTGGAAC ATCTGGAGTG CCTGGTGTCC
    CGCCATGAGC GGTCACTGAG GATGACTGTG GTGAAGCGTC AGGCCCAGTC ACCCTCAGGG
    GTCTCCAGTG AGGTGGAGGT GCTGAAGGCT CTCAAGTCAC TGTTTGAGCA CCACAAGGCC
    CTGGATGAGA AGGTGCGAGA GCGGCTCCGG GTGGCCCTCG AGAGAGTGAC CACTTTGGAG
    GAACAGCTGG CGTGTGCCCA CCAGCAGGTG TCTTCCCTAC AGCAGGGGTC AGGGGTTCGG
    GATGGAGTGG CCGAAGAGGA GGGGACTGTG GAGCTGGGAT CAAAAGGCCT GTGGAAGGAG
    GACACAGGCC AGGTTGAGGA GCTGCAGGAG CTTCTGGAGA AGCAGAACTT TGAGTTGAGC
    CAGGCCCGGG AGCGACTGGT CACCCTGACA GCAACTGTGG CTGAACTGGA GGAGGACCTG
    GGCACAGCCC GCCGGGACCT CATCAAGTCG GAGGAACTGA GCAGCAAACA CCAGCGGGAC
    CTCCGGGAAG CTCTAGCCCA GAAAGAGGAC ATGGAGGAGC GGATCACCAC CCTGGAGAAG
    CGCTACCTGG CTGCTCAGCG TGAGGCTACA TCAATACATG ACCTCAATGA CAAACTGGAG
    AACGAGCTAG CCAATAGGGA ATCCTTGCAT CGCCAGTGTG AGGAGAAAGC CCGATATCTG
    CAGGAGCTAC TGGAGGTAGC CGAGCAGAAA CTTCAGCAGA CAATGCGCAA GGCCGAGACG
    CTGCCAGAGG TGGAGGCTGA GCTGGCCCAA AGAGTTGCGG CCCTCACCAA GGCTGAAGAG
    CGACATGGCA ACACTGAGGA GCACCTCCGA CAGCTGGAGG GACAGCTGGA GGAGAAGAAC
    CAAGAGCTGG CGCGGGTGCG CCAGCGGGAG AGGATGAATG AAGCCCACAA CAAGCGCCTG
    TCAGACACTG TGGACCGGCT GCTTATCGAG GCGAAGGAGC GCCTGCAGCT CCACCTCAGG
    GAGCGCATGG TGGCCCTGGA GGAGAAGAAC ACATTGATCC AAGAGCTGGA GACCTCCCAG
    CGGCAGATTG AAGAGCAGCA CCATCACAAG GGCCGCCTGT CTGAAGAGAT TGAGAAACTG
    CGCCAAGAGG TGGACCAACT GAAGGGTCGA GGAGGACCAT TTGTGGATGG CATCCACTCC
    AGGGCGTACA CGGGCAGTGC AATGGATGTG CGGTTCTCCC TGAGCACAAC TGCCCACACA
    TCTCAAGGTC TGCGTCGTTG TTACTCAGCA CGGCGGGAAC CGCCTGCCAA GGACTGGGAG
    CCATCTCCAC GGCCTGCGGT GCTGGTCCCG ACAACCACCC CTGCCTTTGA CAGTGACCGT
    GAGATCTCTG ATGTGGATGA GGATGAACCA GGGGGTCTGG TGGGCTCCAT GGACGTTGTC
    TCCCCCAGCA GCCACTCAGA CGCCCAGACC CTGGCCATGA TGCTGCAGGA GCAGCTGGAT
    GCTATCAACG AGGAGATCAG GATGATCCAG GAAGAGAAGG AGTCCACAGA GCTCCGTGCT
    GAGGAGCTGG AAACTCGAGT GACTAGTGGC AGCATGGAGG CCCTAGACCT GACCCAGCTG
    CACAAACGCG GTTCCATCCC CACCTCTCTG ACCGCCCTGT CCCTGGCCAG CGCGTCCCCT
    CCACTTAGCG GCCGTGCCAC ACCTAAGCTC ACCTCCCGCA GTGCTGCCCA GGACCTGGAT
    CGGATGGGGG TCATGACCTT GCCCAGTGAC TTAAGAAAGC ATAGGAGGAA GCTGCTGTCA
    CCAGTGTCTT GGGAAGAGAA CCGAGAGGAT AAAGCCACCA TAAAATGTGA GACTTCCCCT
    CCTTCCTCAC CCAGGACACT GCAGCTAGAG AAGCTTGGCC TCCCTGCCCT GAGCCAGGAA
    GAAGGCAAGA GTGCTTTGGA GGATCCAGGC AGCAACCCCA GCAGCAGCAA CAGCAGCCAG
    GACTCCTTGC ACAAGGGTGC CAAGCGCAAG GGCATCAAAT CATCCATCGG CCGCCTGTTT
    GGGAAGAAGG AGAAAGGCAG GCTGATCCAG CCGAGCCGGG ATGGACCCAC AGGCCATGTT
    GTACTACTAA CAGACTCTGA GCTCGGTCTG CAGGAGCCCA TGGTGCCTGG CAAGCTGGGA
    ACCCAGGCAG AAAAGGACCG GAGGCTGAAG AAGAAACACC AGCTGCTTGA AGATGCCCGG
    AGAAAAGGAA TGCCCTTTGC CCAGTGGGAC GGCCCTACTG TGGTCTCCTG GCTAGAGCTC
    TGGGTGGGGA TGCCTGCTTG GTATGTGGCA GCCTGCCGGG CCAACGTCAA GAGTGGCGCC
    ATCATGTCAG CCCTATCGGA CACAGAGATC CAGCGAGAAA TTGGCATCAG CAATGCCCTG
    CACCGGCTCA AGCTCCGGCT GGCCATCCAG GAGATGGTGT CGCTGACCAG CCCCTCTGCC
    CCGCCCACCT CCAGGACTTC TTCTGGGAAT GTCTGGGTCA CCCACGAAGA GATGGAAACT
    CTGGCAACGT CCACTAAAAC AGACAGTGAG GAGGGCAGCT GGGCTCAGAC CCTGGTCTAT
    GGGGATATGA ACCACGAGTG GATTGGGAAC CAGTGGCTCC CCAGCCTGGG GCTCCCCCAG
    TACCGCAGCT ACTTCATGGA GTGCCTCGTG GACGCTCGCA TGCTGGATCA CCTCACCAAG
    AAGGACTTGC GGGTCCACCT GAAGATGGTG GACAGCTCCC ACCGAACCAG TCTTCAGTAT
    GGCATCATGT GCCTGAAGAG GCTGAATTAT GACCGGAAGG AGCTGGAGAA GCGGCGGGAG
    GAGAGCCAGC ATGAGATCAA GGATGTGCTG GTCTGGACCA ATGACCAGGT AGTTCATTGG
    GTCCAGTCTA TTGGGCTCCG AGACTACGCA GGAAACCTCC ATGAGAGTGG TGTGCATGGC
    GCTTTGCTGG CCCTGGATGA GAACTTTGAC CACAACACAC TGGCCCTGGT TCTCCAGATA
    CCCACACAAA ATACCCAGGC ACGCCAAGTG TTGGAGAGAG AGTTCAACAA CCTGTTGGCT
    TTGGGCACAG ACCGGAAGCT GGATGATGGG GAAGACAAGG CGCTACGCCG CGCGCCCTCC
    TGGAGGAAAC GCTTCCGCCC GCGGGACCCC CACGGCCGCG CCTCGCTCAG CGCCGCGGCC
    GACACGCTCC CGGCAACCTT CCGCGCGTCC ACCGTGGGGC CCGTGCCGCC TCCAGCATCC
    CCGCGGAAGA AGATCACGCC TGAAGGTCTG GACCGCTCAC TCCACCGGAG ACCAGGGTGC
    AGAACCAAGG GCTGGGGTGG CCCTTCCAAA GAAAGGGCGC TTCAGACTTC CTTGCTTGGG
    TTATTGGGCC TTCTGGTTTC TGTCCGTCTC TTGGTTGCAC TAGATGGTCT ACGTCTGCTT
    TTGGCATCCC CCCTCCCGCC ATTTTCTCTC CCCCAACTCA TCCGCACCTT GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu41790
    Clone ID Related Accession (Same CDS sequence) XM_025277804.1
    Accession Version XM_025277804.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3891bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product liprin-alpha-4 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_020228958.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGAGGCAG GGCTCCCTTG GGCCCTAAAA CTTGACTGGC GTTGGGTAAA AATCTGGCTC 
    CCCGCCACCT CACGAGATAC TCTCCCGGCC GAGCCAAGAT GGCGCGCAGC CCCAAGTGGC
    TCCACGTCAC GAGGGAGGGA GCGCCGGGGC GGGGGCGTGG CCTCGAGGGG GCCGGCTAGC
    CCCGCCCTTG AGGTGGGGCC GGGGGCCGTC CGCAGAAGGC GAGGGCGGGG CGCTTCGGCT
    CGGGAAGGCT GCGGCTGCCT CGGCGCCGGC TCCGCTCGCG AGGACAAGTC CGGCGCCTCC
    AGGCCGGGCC GGCTTCCTGC GCAGAGCCAA GCTGCCCGGC CTTCGGAGTT TACCACCTTA
    ACCCGGGAGC TGAGTATGTG TCGGGAGCAG CTTCTAGAGA GGGAGGAAGA GATATCAGAG
    CTGAAAGCAG AGCGGAATAA CACGAGGTTG CTTCTGGAAC ATCTGGAGTG CCTGGTGTCC
    CGCCATGAGC GGTCACTGAG GATGACTGTG GTGAAGCGTC AGGCCCAGTC ACCCTCAGGG
    GTCTCCAGTG AGGTGGAGGT GCTGAAGGCT CTCAAGTCAC TGTTTGAGCA CCACAAGGCC
    CTGGATGAGA AGGTGCGAGA GCGGCTCCGG GTGGCCCTCG AGAGAGTGAC CACTTTGGAG
    GAACAGCTGG CGTGTGCCCA CCAGCAGGTG TCTTCCCTAC AGCAGGGGTC AGGGGTTCGG
    GATGGAGTGG CCGAAGAGGA GGGGACTGTG GAGCTGGGAT CAAAAGGCCT GTGGAAGGAC
    ACAGGCCAGG TTGAGGAGCT GCAGGAGCTT CTGGAGAAGC AGAACTTTGA GTTGAGCCAG
    GCCCGGGAGC GACTGGTCAC CCTGACAGCA ACTGTGGCTG AACTGGAGGA GGACCTGGGC
    ACAGCCCGCC GGGACCTCAT CAAGTCGGAG GAACTGAGCA GCAAACACCA GCGGGACCTC
    CGGGAAGCTC TAGCCCAGAA AGAGGACATG GAGGAGCGGA TCACCACCCT GGAGAAGCGC
    TACCTGGCTG CTCAGCGTGA GGCTACATCA ATACATGACC TCAATGACAA ACTGGAGAAC
    GAGCTAGCCA ATAGGGAATC CTTGCATCGC CAGTGTGAGG AGAAAGCCCG ATATCTGCAG
    GAGCTACTGG AGGTAGCCGA GCAGAAACTT CAGCAGACAA TGCGCAAGGC CGAGACGCTG
    CCAGAGGTGG AGGCTGAGCT GGCCCAAAGA GTTGCGGCCC TCACCAAGGC TGAAGAGCGA
    CATGGCAACA CTGAGGAGCA CCTCCGACAG CTGGAGGGAC AGCTGGAGGA GAAGAACCAA
    GAGCTGGCGC GGGTGCGCCA GCGGGAGAGG ATGAATGAAG CCCACAACAA GCGCCTGTCA
    GACACTGTGG ACCGGCTGCT TATCGAGGCG AAGGAGCGCC TGCAGCTCCA CCTCAGGGAG
    CGCATGGTGG CCCTGGAGGA GAAGAACACA TTGATCCAAG AGCTGGAGAC CTCCCAGCGG
    CAGATTGAAG AGCAGCACCA TCACAAGGGC CGCCTGTCTG AAGAGATTGA GAAACTGCGC
    CAAGAGGTGG ACCAACTGAA GGGTCGAGGA GGACCATTTG TGGATGGCAT CCACTCCAGG
    GCGTACACGG GCAGTGCAAT GGATGTGCGG TTCTCCCTGA GCACAACTGC CCACACATCT
    CAAGGTCTGC GTCGTTGTTA CTCAGCACGG CGGGAACCGC CTGCCAAGGA CTGGGAGCCA
    TCTCCACGGC CTGCGGTGCT GGTCCCGACA ACCACCCCTG CCTTTGACAG TGACCGTGAG
    ATCTCTGATG TGGATGAGGA TGAACCAGGG GGTCTGGTGG GCTCCATGGA CGTTGTCTCC
    CCCAGCAGCC ACTCAGACGC CCAGACCCTG GCCATGATGC TGCAGGAGCA GCTGGATGCT
    ATCAACGAGG AGATCAGGAT GATCCAGGAA GAGAAGGAGT CCACAGAGCT CCGTGCTGAG
    GAGCTGGAAA CTCGAGTGAC TAGTGGCAGC ATGGAGGCCC TAGACCTGAC CCAGCTGCAC
    AAACGCGGTT CCATCCCCAC CTCTCTGACC GCCCTGTCCC TGGCCAGCGC GTCCCCTCCA
    CTTAGCGGCC GTGCCACACC TAAGCTCACC TCCCGCAGTG CTGCCCAGGA CCTGGATCGG
    ATGGGGGTCA TGACCTTGCC CAGTGACTTA AGAAAGCATA GGAGGAAGCT GCTGTCACCA
    GTGTCTTGGG AAGAGAACCG AGAGGATAAA GCCACCATAA AATGTGAGAC TTCCCCTCCT
    TCCTCACCCA GGACACTGCA GCTAGAGAAG CTTGGCCTCC CTGCCCTGAG CCAGGAAGAA
    GGCAAGAGTG CTTTGGAGGA TCCAGGCAGC AACCCCAGCA GCAGCAACAG CAGCCAGGAC
    TCCTTGCACA AGGGTGCCAA GCGCAAGGGC ATCAAATCAT CCATCGGCCG CCTGTTTGGG
    AAGAAGGAGA AAGGCAGGCT GATCCAGCCG AGCCGGGATG GACCCACAGG CCATGTTGTA
    CTACTAACAG ACTCTGAGCT CGGTCTGCAG GAGCCCATGG TGCCTGGCAA GCTGGGAACC
    CAGGCAGAAA AGGACCGGAG GCTGAAGAAG AAACACCAGC TGCTTGAAGA TGCCCGGAGA
    AAAGGAATGC CCTTTGCCCA GTGGGACGGC CCTACTGTGG TCTCCTGGCT AGAGCTCTGG
    GTGGGGATGC CTGCTTGGTA TGTGGCAGCC TGCCGGGCCA ACGTCAAGAG TGGCGCCATC
    ATGTCAGCCC TATCGGACAC AGAGATCCAG CGAGAAATTG GCATCAGCAA TGCCCTGCAC
    CGGCTCAAGC TCCGGCTGGC CATCCAGGAG ATGGTGTCGC TGACCAGCCC CTCTGCCCCG
    CCCACCTCCA GGACTTCTTC TGGGAATGTC TGGGTCACCC ACGAAGAGAT GGAAACTCTG
    GCAACGTCCA CTAAAACAGA CAGTGAGGAG GGCAGCTGGG CTCAGACCCT GGTCTATGGG
    GATATGAACC ACGAGTGGAT TGGGAACCAG TGGCTCCCCA GCCTGGGGCT CCCCCAGTAC
    CGCAGCTACT TCATGGAGTG CCTCGTGGAC GCTCGCATGC TGGATCACCT CACCAAGAAG
    GACTTGCGGG TCCACCTGAA GATGGTGGAC AGCTCCCACC GAACCAGTCT TCAGTATGGC
    ATCATGTGCC TGAAGAGGCT GAATTATGAC CGGAAGGAGC TGGAGAAGCG GCGGGAGGAG
    AGCCAGCATG AGATCAAGGA TGTGCTGGTC TGGACCAATG ACCAGGTAGT TCATTGGGTC
    CAGTCTATTG GGCTCCGAGA CTACGCAGGA AACCTCCATG AGAGTGGTGT GCATGGCGCT
    TTGCTGGCCC TGGATGAGAA CTTTGACCAC AACACACTGG CCCTGGTTCT CCAGATACCC
    ACACAAAATA CCCAGGCACG CCAAGTGTTG GAGAGAGAGT TCAACAACCT GTTGGCTTTG
    GGCACAGACC GGAAGCTGGA TGATGGGGAA GACAAGGCGC TACGCCGCGC GCCCTCCTGG
    AGGAAACGCT TCCGCCCGCG GGACCCCCAC GGCCGCGCCT CGCTCAGCGC CGCGGCCGAC
    ACGCTCCCGG CAACCTTCCG CGCGTCCACC GTGGGGCCCG TGCCGCCTCC AGCATCCCCG
    CGGAAGAAGA TCACGCCTGA AGGTCTGGAC CGCTCACTCC ACCGGAGACC AGGGTGCAGA
    ACCAAGGGCT GGGGTGGCCC TTCCAAAGAA AGGGCGCTTC AGACTTCCTT GCTTGGGTTA
    TTGGGCCTTC TGGTTTCTGT CCGTCTCTTG GTTGCACTAG ATGGTCTACG TCTGCTTTTG
    GCATCCCCCC TCCCGCCATT TTCTCTCCCC CAACTCATCC GCACCTTGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025133589.1
    CDS564..4454
    Translation

    Target ORF information:

    RefSeq Version XM_025277804.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis PTPRF interacting protein alpha 4 (PPFIA4), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025277804.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGAGGCAG GGCTCCCTTG GGCCCTAAAA CTTGACTGGC GTTGGGTAAA AATCTGGCTC 
    CCCGCCACCT CACGAGATAC TCTCCCGGCC GAGCCAAGAT GGCGCGCAGC CCCAAGTGGC
    TCCACGTCAC GAGGGAGGGA GCGCCGGGGC GGGGGCGTGG CCTCGAGGGG GCCGGCTAGC
    CCCGCCCTTG AGGTGGGGCC GGGGGCCGTC CGCAGAAGGC GAGGGCGGGG CGCTTCGGCT
    CGGGAAGGCT GCGGCTGCCT CGGCGCCGGC TCCGCTCGCG AGGACAAGTC CGGCGCCTCC
    AGGCCGGGCC GGCTTCCTGC GCAGAGCCAA GCTGCCCGGC CTTCGGAGTT TACCACCTTA
    ACCCGGGAGC TGAGTATGTG TCGGGAGCAG CTTCTAGAGA GGGAGGAAGA GATATCAGAG
    CTGAAAGCAG AGCGGAATAA CACGAGGTTG CTTCTGGAAC ATCTGGAGTG CCTGGTGTCC
    CGCCATGAGC GGTCACTGAG GATGACTGTG GTGAAGCGTC AGGCCCAGTC ACCCTCAGGG
    GTCTCCAGTG AGGTGGAGGT GCTGAAGGCT CTCAAGTCAC TGTTTGAGCA CCACAAGGCC
    CTGGATGAGA AGGTGCGAGA GCGGCTCCGG GTGGCCCTCG AGAGAGTGAC CACTTTGGAG
    GAACAGCTGG CGTGTGCCCA CCAGCAGGTG TCTTCCCTAC AGCAGGGGTC AGGGGTTCGG
    GATGGAGTGG CCGAAGAGGA GGGGACTGTG GAGCTGGGAT CAAAAGGCCT GTGGAAGGAC
    ACAGGCCAGG TTGAGGAGCT GCAGGAGCTT CTGGAGAAGC AGAACTTTGA GTTGAGCCAG
    GCCCGGGAGC GACTGGTCAC CCTGACAGCA ACTGTGGCTG AACTGGAGGA GGACCTGGGC
    ACAGCCCGCC GGGACCTCAT CAAGTCGGAG GAACTGAGCA GCAAACACCA GCGGGACCTC
    CGGGAAGCTC TAGCCCAGAA AGAGGACATG GAGGAGCGGA TCACCACCCT GGAGAAGCGC
    TACCTGGCTG CTCAGCGTGA GGCTACATCA ATACATGACC TCAATGACAA ACTGGAGAAC
    GAGCTAGCCA ATAGGGAATC CTTGCATCGC CAGTGTGAGG AGAAAGCCCG ATATCTGCAG
    GAGCTACTGG AGGTAGCCGA GCAGAAACTT CAGCAGACAA TGCGCAAGGC CGAGACGCTG
    CCAGAGGTGG AGGCTGAGCT GGCCCAAAGA GTTGCGGCCC TCACCAAGGC TGAAGAGCGA
    CATGGCAACA CTGAGGAGCA CCTCCGACAG CTGGAGGGAC AGCTGGAGGA GAAGAACCAA
    GAGCTGGCGC GGGTGCGCCA GCGGGAGAGG ATGAATGAAG CCCACAACAA GCGCCTGTCA
    GACACTGTGG ACCGGCTGCT TATCGAGGCG AAGGAGCGCC TGCAGCTCCA CCTCAGGGAG
    CGCATGGTGG CCCTGGAGGA GAAGAACACA TTGATCCAAG AGCTGGAGAC CTCCCAGCGG
    CAGATTGAAG AGCAGCACCA TCACAAGGGC CGCCTGTCTG AAGAGATTGA GAAACTGCGC
    CAAGAGGTGG ACCAACTGAA GGGTCGAGGA GGACCATTTG TGGATGGCAT CCACTCCAGG
    GCGTACACGG GCAGTGCAAT GGATGTGCGG TTCTCCCTGA GCACAACTGC CCACACATCT
    CAAGGTCTGC GTCGTTGTTA CTCAGCACGG CGGGAACCGC CTGCCAAGGA CTGGGAGCCA
    TCTCCACGGC CTGCGGTGCT GGTCCCGACA ACCACCCCTG CCTTTGACAG TGACCGTGAG
    ATCTCTGATG TGGATGAGGA TGAACCAGGG GGTCTGGTGG GCTCCATGGA CGTTGTCTCC
    CCCAGCAGCC ACTCAGACGC CCAGACCCTG GCCATGATGC TGCAGGAGCA GCTGGATGCT
    ATCAACGAGG AGATCAGGAT GATCCAGGAA GAGAAGGAGT CCACAGAGCT CCGTGCTGAG
    GAGCTGGAAA CTCGAGTGAC TAGTGGCAGC ATGGAGGCCC TAGACCTGAC CCAGCTGCAC
    AAACGCGGTT CCATCCCCAC CTCTCTGACC GCCCTGTCCC TGGCCAGCGC GTCCCCTCCA
    CTTAGCGGCC GTGCCACACC TAAGCTCACC TCCCGCAGTG CTGCCCAGGA CCTGGATCGG
    ATGGGGGTCA TGACCTTGCC CAGTGACTTA AGAAAGCATA GGAGGAAGCT GCTGTCACCA
    GTGTCTTGGG AAGAGAACCG AGAGGATAAA GCCACCATAA AATGTGAGAC TTCCCCTCCT
    TCCTCACCCA GGACACTGCA GCTAGAGAAG CTTGGCCTCC CTGCCCTGAG CCAGGAAGAA
    GGCAAGAGTG CTTTGGAGGA TCCAGGCAGC AACCCCAGCA GCAGCAACAG CAGCCAGGAC
    TCCTTGCACA AGGGTGCCAA GCGCAAGGGC ATCAAATCAT CCATCGGCCG CCTGTTTGGG
    AAGAAGGAGA AAGGCAGGCT GATCCAGCCG AGCCGGGATG GACCCACAGG CCATGTTGTA
    CTACTAACAG ACTCTGAGCT CGGTCTGCAG GAGCCCATGG TGCCTGGCAA GCTGGGAACC
    CAGGCAGAAA AGGACCGGAG GCTGAAGAAG AAACACCAGC TGCTTGAAGA TGCCCGGAGA
    AAAGGAATGC CCTTTGCCCA GTGGGACGGC CCTACTGTGG TCTCCTGGCT AGAGCTCTGG
    GTGGGGATGC CTGCTTGGTA TGTGGCAGCC TGCCGGGCCA ACGTCAAGAG TGGCGCCATC
    ATGTCAGCCC TATCGGACAC AGAGATCCAG CGAGAAATTG GCATCAGCAA TGCCCTGCAC
    CGGCTCAAGC TCCGGCTGGC CATCCAGGAG ATGGTGTCGC TGACCAGCCC CTCTGCCCCG
    CCCACCTCCA GGACTTCTTC TGGGAATGTC TGGGTCACCC ACGAAGAGAT GGAAACTCTG
    GCAACGTCCA CTAAAACAGA CAGTGAGGAG GGCAGCTGGG CTCAGACCCT GGTCTATGGG
    GATATGAACC ACGAGTGGAT TGGGAACCAG TGGCTCCCCA GCCTGGGGCT CCCCCAGTAC
    CGCAGCTACT TCATGGAGTG CCTCGTGGAC GCTCGCATGC TGGATCACCT CACCAAGAAG
    GACTTGCGGG TCCACCTGAA GATGGTGGAC AGCTCCCACC GAACCAGTCT TCAGTATGGC
    ATCATGTGCC TGAAGAGGCT GAATTATGAC CGGAAGGAGC TGGAGAAGCG GCGGGAGGAG
    AGCCAGCATG AGATCAAGGA TGTGCTGGTC TGGACCAATG ACCAGGTAGT TCATTGGGTC
    CAGTCTATTG GGCTCCGAGA CTACGCAGGA AACCTCCATG AGAGTGGTGT GCATGGCGCT
    TTGCTGGCCC TGGATGAGAA CTTTGACCAC AACACACTGG CCCTGGTTCT CCAGATACCC
    ACACAAAATA CCCAGGCACG CCAAGTGTTG GAGAGAGAGT TCAACAACCT GTTGGCTTTG
    GGCACAGACC GGAAGCTGGA TGATGGGGAA GACAAGGCGC TACGCCGCGC GCCCTCCTGG
    AGGAAACGCT TCCGCCCGCG GGACCCCCAC GGCCGCGCCT CGCTCAGCGC CGCGGCCGAC
    ACGCTCCCGG CAACCTTCCG CGCGTCCACC GTGGGGCCCG TGCCGCCTCC AGCATCCCCG
    CGGAAGAAGA TCACGCCTGA AGGTCTGGAC CGCTCACTCC ACCGGAGACC AGGGTGCAGA
    ACCAAGGGCT GGGGTGGCCC TTCCAAAGAA AGGGCGCTTC AGACTTCCTT GCTTGGGTTA
    TTGGGCCTTC TGGTTTCTGT CCGTCTCTTG GTTGCACTAG ATGGTCTACG TCTGCTTTTG
    GCATCCCCCC TCCCGCCATT TTCTCTCCCC CAACTCATCC GCACCTTGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu41791
    Clone ID Related Accession (Same CDS sequence) XM_025277805.1
    Accession Version XM_025277805.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3867bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product liprin-alpha-4 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_020228958.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGAGGCAG GGCTCCCTTG GGCCCTAAAA CTTGACTGGC GTTGGGTAAA AATCTGGCTC 
    CCCGCCACCT CACGAGATAC TCTCCCGGCC GAGCCAAGAT GGCGCGCAGC CCCAAGTGGC
    TCCACGTCAC GAGGGAGGGA GCGCCGGGGC GGGGGCGTGG CCTCGAGGGG GCCGGCTAGC
    CCCGCCCTTG AGGTGGGGCC GGGGGCCGTC CGCAGAAGGC GAGGGCGGGG CGCTTCGGCT
    CGGGAAGGCT GCGGCTGCCT CGGCGCCGGC TCCGCTCGCG AGGACAAGTC CGGCGCCTCC
    AGGCCGGGCC GGCTTCCTGC GCAGAGCCAA GCTGCCCGGC CTTCGGAGTT TACCACCTTA
    ACCCGGGAGC TGAGTATGTG TCGGGAGCAG CTTCTAGAGA GGGAGGAAGA GATATCAGAG
    CTGAAAGCAG AGCGGAATAA CACGAGGTTG CTTCTGGAAC ATCTGGAGTG CCTGGTGTCC
    CGCCATGAGC GGTCACTGAG GATGACTGTG GTGAAGCGTC AGGCCCAGTC ACCCTCAGGG
    GTCTCCAGTG AGGTGGAGGT GCTGAAGGCT CTCAAGTCAC TGTTTGAGCA CCACAAGGCC
    CTGGATGAGA AGGTGCGAGA GCGGCTCCGG GTGGCCCTCG AGAGAGTGAC CACTTTGGAG
    GAACAGCTGG CGTGTGCCCA CCAGCAGGTG TCTTCCCTAC AGCAGGGGTC AGGGGTTCGG
    GATGGAGTGG CCGAAGAGGA GGGGACTGTG GAGCTGGGAT CAAAAGGCCT GTGGAAGGAG
    GACACAGGCC AGGTTGAGGA GCTGCAGGAG CTTCTGGAGA AGCAGAACTT TGAGTTGAGC
    CAGGCCCGGG AGCGACTGGT CACCCTGACA GCAACTGTGG CTGAACTGGA GGAGGACCTG
    GGCACAGCCC GCCGGGACCT CATCAAGTCG GAGGAACTGA GCAGCAAACA CCAGCGGGAC
    CTCCGGGAAG CTCTAGCCCA GAAAGAGGAC ATGGAGGAGC GGATCACCAC CCTGGAGAAG
    CGCTACCTGG CTGCTCAGCG TGAGGCTACA TCAATACATG ACCTCAATGA CAAACTGGAG
    AACGAGCTAG CCAATAGGGA ATCCTTGCAT CGCCAGTGTG AGGAGAAAGC CCGATATCTG
    CAGGAGCTAC TGGAGGTAGC CGAGCAGAAA CTTCAGCAGA CAATGCGCAA GGCCGAGACG
    CTGCCAGAGG TGGAGGCTGA GCTGGCCCAA AGAGTTGCGG CCCTCACCAA GGCTGAAGAG
    CGACATGGCA ACACTGAGGA GCACCTCCGA CAGCTGGAGG GACAGCTGGA GGAGAAGAAC
    CAAGAGCTGG CGCGGGTGCG CCAGCGGGAG AGGATGAATG AAGCCCACAA CAAGCGCCTG
    TCAGACACTG TGGACCGGCT GCTTATCGAG GCGAAGGAGC GCCTGCAGCT CCACCTCAGG
    GAGCGCATGG TGGCCCTGGA GGAGAAGAAC ACATTGATCC AAGAGCTGGA GACCTCCCAG
    CGGCAGATTG AAGAGCAGCA CCATCACAAG GGCCGCCTGT CTGAAGAGAT TGAGAAACTG
    CGCCAAGAGG TGGACCAACT GAAGGGTCGA GGAGGACCAT TTGTGGATGG CATCCACTCC
    AGGGCGTACA CGGGCAGTGC AATGGATGTG CGGTTCTCCC TGAGCACAAC TGCCCACACA
    TCTCAAGGTC TGCGTCGTTG TTACTCAGCA CGGCGGGAAC CGCCTGCCAA GGACTGGGAG
    CCATCTCCAC GGCCTGCGGT GCTGGTCCCG ACAACCACCC CTGCCTTTGA CAGTGACCGT
    GAGATCTCTG ATGTGGATGA GGATGAACCA GGGGGTCTGG TGGGCTCCAT GGACGTTGTC
    TCCCCCAGCA GCCACTCAGA CGCCCAGACC CTGGCCATGA TGCTGCAGGA GCAGCTGGAT
    GCTATCAACG AGGAGATCAG GATGATCCAG GAAGAGAAGG AGTCCACAGA GCTCCGTGCT
    GAGGAGCTGG AAACTCGAGT GACTAGTGGC AGCATGGAGG CCCTAGACCT GACCCAGCTG
    CACAAACGCG GTTCCATCCC CACCTCTCTG ACCGCCCTGT CCCTGGCCAG CGCGTCCCCT
    CCACTTAGCG GCCGTGCCAC ACCTAAGCTC ACCTCCCGCA GTGCTGCCCA GGACCTGGAT
    CGGATGGGGG TCATGACCTT GCCCAGTGAC TTAAGAAAGC ATAGGAGGAA GCTGCTGTCA
    CCAGTGTCTT GGGAAGAGAA CCGAGAGGAT AAAGCCACCA TAAAATGTGA GACTTCCCCT
    CCTTCCTCAC CCAGGACACT GCAGCTAGAG AAGCTTGGCC TCCCTGCCCT GAGCCAGGAA
    GAAGGCAAGA GTGCTTTGGA GGATCCAGGC AGCAACCCCA GCAGCAGCAA CAGCAGCCAG
    GACTCCTTGC ACAAGGGTGC CAAGCGCAAG GGCATCAAAT CATCCATCGG CCGCCTGTTT
    GGGAAGAAGG AGAAAGGCAG GCTGATCCAG CCGAGCCGGG ATGGACCCAC AGGCCATGTT
    GTACTACTAA CAGACTCTGA GCTCGGTCTG CAGGAGCCCA TGGTGCCTGG CAAGCTGGGA
    ACCCAGGCAG AAAAGGACCG GAGGCTGAAG AAGAAACACC AGCTGCTTGA AGATGCCCGG
    AGAAAAGGAA TGCCCTTTGC CCAGTGGGAC GGCCCTACTG TGGTCTCCTG GCTAGAGCTC
    TGGGTGGGGA TGCCTGCTTG GTATGTGGCA GCCTGCCGGG CCAACGTCAA GAGTGGCGCC
    ATCATGTCAG CCCTATCGGA CACAGAGATC CAGCGAGAAA TTGGCATCAG CAATGCCCTG
    CACCGGCTCA AGCTCCGGCT GGCCATCCAG GAGATGGTGT CGCTGACCAG CCCCTCTGCC
    CCGCCCACCT CCAGGACTTC TTCTGGGAAT GTCTGGGTCA CCCACGAAGA GATGGAAACT
    CTGGCAACGT CCACTAAAAC AACCCTGGTC TATGGGGATA TGAACCACGA GTGGATTGGG
    AACCAGTGGC TCCCCAGCCT GGGGCTCCCC CAGTACCGCA GCTACTTCAT GGAGTGCCTC
    GTGGACGCTC GCATGCTGGA TCACCTCACC AAGAAGGACT TGCGGGTCCA CCTGAAGATG
    GTGGACAGCT CCCACCGAAC CAGTCTTCAG TATGGCATCA TGTGCCTGAA GAGGCTGAAT
    TATGACCGGA AGGAGCTGGA GAAGCGGCGG GAGGAGAGCC AGCATGAGAT CAAGGATGTG
    CTGGTCTGGA CCAATGACCA GGTAGTTCAT TGGGTCCAGT CTATTGGGCT CCGAGACTAC
    GCAGGAAACC TCCATGAGAG TGGTGTGCAT GGCGCTTTGC TGGCCCTGGA TGAGAACTTT
    GACCACAACA CACTGGCCCT GGTTCTCCAG ATACCCACAC AAAATACCCA GGCACGCCAA
    GTGTTGGAGA GAGAGTTCAA CAACCTGTTG GCTTTGGGCA CAGACCGGAA GCTGGATGAT
    GGGGAAGACA AGGCGCTACG CCGCGCGCCC TCCTGGAGGA AACGCTTCCG CCCGCGGGAC
    CCCCACGGCC GCGCCTCGCT CAGCGCCGCG GCCGACACGC TCCCGGCAAC CTTCCGCGCG
    TCCACCGTGG GGCCCGTGCC GCCTCCAGCA TCCCCGCGGA AGAAGATCAC GCCTGAAGGT
    CTGGACCGCT CACTCCACCG GAGACCAGGG TGCAGAACCA AGGGCTGGGG TGGCCCTTCC
    AAAGAAAGGG CGCTTCAGAC TTCCTTGCTT GGGTTATTGG GCCTTCTGGT TTCTGTCCGT
    CTCTTGGTTG CACTAGATGG TCTACGTCTG CTTTTGGCAT CCCCCCTCCC GCCATTTTCT
    CTCCCCCAAC TCATCCGCAC CTTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025133590.1
    CDS563..4429
    Translation

    Target ORF information:

    RefSeq Version XM_025277805.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis PTPRF interacting protein alpha 4 (PPFIA4), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025277805.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGAGGCAG GGCTCCCTTG GGCCCTAAAA CTTGACTGGC GTTGGGTAAA AATCTGGCTC 
    CCCGCCACCT CACGAGATAC TCTCCCGGCC GAGCCAAGAT GGCGCGCAGC CCCAAGTGGC
    TCCACGTCAC GAGGGAGGGA GCGCCGGGGC GGGGGCGTGG CCTCGAGGGG GCCGGCTAGC
    CCCGCCCTTG AGGTGGGGCC GGGGGCCGTC CGCAGAAGGC GAGGGCGGGG CGCTTCGGCT
    CGGGAAGGCT GCGGCTGCCT CGGCGCCGGC TCCGCTCGCG AGGACAAGTC CGGCGCCTCC
    AGGCCGGGCC GGCTTCCTGC GCAGAGCCAA GCTGCCCGGC CTTCGGAGTT TACCACCTTA
    ACCCGGGAGC TGAGTATGTG TCGGGAGCAG CTTCTAGAGA GGGAGGAAGA GATATCAGAG
    CTGAAAGCAG AGCGGAATAA CACGAGGTTG CTTCTGGAAC ATCTGGAGTG CCTGGTGTCC
    CGCCATGAGC GGTCACTGAG GATGACTGTG GTGAAGCGTC AGGCCCAGTC ACCCTCAGGG
    GTCTCCAGTG AGGTGGAGGT GCTGAAGGCT CTCAAGTCAC TGTTTGAGCA CCACAAGGCC
    CTGGATGAGA AGGTGCGAGA GCGGCTCCGG GTGGCCCTCG AGAGAGTGAC CACTTTGGAG
    GAACAGCTGG CGTGTGCCCA CCAGCAGGTG TCTTCCCTAC AGCAGGGGTC AGGGGTTCGG
    GATGGAGTGG CCGAAGAGGA GGGGACTGTG GAGCTGGGAT CAAAAGGCCT GTGGAAGGAG
    GACACAGGCC AGGTTGAGGA GCTGCAGGAG CTTCTGGAGA AGCAGAACTT TGAGTTGAGC
    CAGGCCCGGG AGCGACTGGT CACCCTGACA GCAACTGTGG CTGAACTGGA GGAGGACCTG
    GGCACAGCCC GCCGGGACCT CATCAAGTCG GAGGAACTGA GCAGCAAACA CCAGCGGGAC
    CTCCGGGAAG CTCTAGCCCA GAAAGAGGAC ATGGAGGAGC GGATCACCAC CCTGGAGAAG
    CGCTACCTGG CTGCTCAGCG TGAGGCTACA TCAATACATG ACCTCAATGA CAAACTGGAG
    AACGAGCTAG CCAATAGGGA ATCCTTGCAT CGCCAGTGTG AGGAGAAAGC CCGATATCTG
    CAGGAGCTAC TGGAGGTAGC CGAGCAGAAA CTTCAGCAGA CAATGCGCAA GGCCGAGACG
    CTGCCAGAGG TGGAGGCTGA GCTGGCCCAA AGAGTTGCGG CCCTCACCAA GGCTGAAGAG
    CGACATGGCA ACACTGAGGA GCACCTCCGA CAGCTGGAGG GACAGCTGGA GGAGAAGAAC
    CAAGAGCTGG CGCGGGTGCG CCAGCGGGAG AGGATGAATG AAGCCCACAA CAAGCGCCTG
    TCAGACACTG TGGACCGGCT GCTTATCGAG GCGAAGGAGC GCCTGCAGCT CCACCTCAGG
    GAGCGCATGG TGGCCCTGGA GGAGAAGAAC ACATTGATCC AAGAGCTGGA GACCTCCCAG
    CGGCAGATTG AAGAGCAGCA CCATCACAAG GGCCGCCTGT CTGAAGAGAT TGAGAAACTG
    CGCCAAGAGG TGGACCAACT GAAGGGTCGA GGAGGACCAT TTGTGGATGG CATCCACTCC
    AGGGCGTACA CGGGCAGTGC AATGGATGTG CGGTTCTCCC TGAGCACAAC TGCCCACACA
    TCTCAAGGTC TGCGTCGTTG TTACTCAGCA CGGCGGGAAC CGCCTGCCAA GGACTGGGAG
    CCATCTCCAC GGCCTGCGGT GCTGGTCCCG ACAACCACCC CTGCCTTTGA CAGTGACCGT
    GAGATCTCTG ATGTGGATGA GGATGAACCA GGGGGTCTGG TGGGCTCCAT GGACGTTGTC
    TCCCCCAGCA GCCACTCAGA CGCCCAGACC CTGGCCATGA TGCTGCAGGA GCAGCTGGAT
    GCTATCAACG AGGAGATCAG GATGATCCAG GAAGAGAAGG AGTCCACAGA GCTCCGTGCT
    GAGGAGCTGG AAACTCGAGT GACTAGTGGC AGCATGGAGG CCCTAGACCT GACCCAGCTG
    CACAAACGCG GTTCCATCCC CACCTCTCTG ACCGCCCTGT CCCTGGCCAG CGCGTCCCCT
    CCACTTAGCG GCCGTGCCAC ACCTAAGCTC ACCTCCCGCA GTGCTGCCCA GGACCTGGAT
    CGGATGGGGG TCATGACCTT GCCCAGTGAC TTAAGAAAGC ATAGGAGGAA GCTGCTGTCA
    CCAGTGTCTT GGGAAGAGAA CCGAGAGGAT AAAGCCACCA TAAAATGTGA GACTTCCCCT
    CCTTCCTCAC CCAGGACACT GCAGCTAGAG AAGCTTGGCC TCCCTGCCCT GAGCCAGGAA
    GAAGGCAAGA GTGCTTTGGA GGATCCAGGC AGCAACCCCA GCAGCAGCAA CAGCAGCCAG
    GACTCCTTGC ACAAGGGTGC CAAGCGCAAG GGCATCAAAT CATCCATCGG CCGCCTGTTT
    GGGAAGAAGG AGAAAGGCAG GCTGATCCAG CCGAGCCGGG ATGGACCCAC AGGCCATGTT
    GTACTACTAA CAGACTCTGA GCTCGGTCTG CAGGAGCCCA TGGTGCCTGG CAAGCTGGGA
    ACCCAGGCAG AAAAGGACCG GAGGCTGAAG AAGAAACACC AGCTGCTTGA AGATGCCCGG
    AGAAAAGGAA TGCCCTTTGC CCAGTGGGAC GGCCCTACTG TGGTCTCCTG GCTAGAGCTC
    TGGGTGGGGA TGCCTGCTTG GTATGTGGCA GCCTGCCGGG CCAACGTCAA GAGTGGCGCC
    ATCATGTCAG CCCTATCGGA CACAGAGATC CAGCGAGAAA TTGGCATCAG CAATGCCCTG
    CACCGGCTCA AGCTCCGGCT GGCCATCCAG GAGATGGTGT CGCTGACCAG CCCCTCTGCC
    CCGCCCACCT CCAGGACTTC TTCTGGGAAT GTCTGGGTCA CCCACGAAGA GATGGAAACT
    CTGGCAACGT CCACTAAAAC AACCCTGGTC TATGGGGATA TGAACCACGA GTGGATTGGG
    AACCAGTGGC TCCCCAGCCT GGGGCTCCCC CAGTACCGCA GCTACTTCAT GGAGTGCCTC
    GTGGACGCTC GCATGCTGGA TCACCTCACC AAGAAGGACT TGCGGGTCCA CCTGAAGATG
    GTGGACAGCT CCCACCGAAC CAGTCTTCAG TATGGCATCA TGTGCCTGAA GAGGCTGAAT
    TATGACCGGA AGGAGCTGGA GAAGCGGCGG GAGGAGAGCC AGCATGAGAT CAAGGATGTG
    CTGGTCTGGA CCAATGACCA GGTAGTTCAT TGGGTCCAGT CTATTGGGCT CCGAGACTAC
    GCAGGAAACC TCCATGAGAG TGGTGTGCAT GGCGCTTTGC TGGCCCTGGA TGAGAACTTT
    GACCACAACA CACTGGCCCT GGTTCTCCAG ATACCCACAC AAAATACCCA GGCACGCCAA
    GTGTTGGAGA GAGAGTTCAA CAACCTGTTG GCTTTGGGCA CAGACCGGAA GCTGGATGAT
    GGGGAAGACA AGGCGCTACG CCGCGCGCCC TCCTGGAGGA AACGCTTCCG CCCGCGGGAC
    CCCCACGGCC GCGCCTCGCT CAGCGCCGCG GCCGACACGC TCCCGGCAAC CTTCCGCGCG
    TCCACCGTGG GGCCCGTGCC GCCTCCAGCA TCCCCGCGGA AGAAGATCAC GCCTGAAGGT
    CTGGACCGCT CACTCCACCG GAGACCAGGG TGCAGAACCA AGGGCTGGGG TGGCCCTTCC
    AAAGAAAGGG CGCTTCAGAC TTCCTTGCTT GGGTTATTGG GCCTTCTGGT TTCTGTCCGT
    CTCTTGGTTG CACTAGATGG TCTACGTCTG CTTTTGGCAT CCCCCCTCCC GCCATTTTCT
    CTCCCCCAAC TCATCCGCAC CTTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu41792
    Clone ID Related Accession (Same CDS sequence) XM_025277806.1
    Accession Version XM_025277806.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3831bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product liprin-alpha-4 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_020228958.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGAGGCAG GGCTCCCTTG GGCCCTAAAA CTTGACTGGC GTTGGGTAAA AATCTGGCTC 
    CCCGCCACCT CACGAGATAC TCTCCCGGCC GAGCCAAGAT GGCGCGCAGC CCCAAGTGGC
    TCCACGTCAC GAGGGAGGGA GCGCCGGGGC GGGGGCGTGG CCTCGAGGGG GCCGGCTAGC
    CCCGCCCTTG AGGTGGGGCC GGGGGCCGTC CGCAGAAGGC GAGGGCGGGG CGCTTCGGCT
    CGGGAAGGCT GCGGCTGCCT CGGCGCCGGC TCCGCTCGCG AGGACAAGTC CGGCGCCTCC
    AGGCCGGGCC GGCTTCCTGC GCAGAGCCAA GCTGCCCGGC CTTCGGAGTT TACCACCTTA
    ACCCGGGAGC TGAGTATGTG TCGGGAGCAG CTTCTAGAGA GGGAGGAAGA GATATCAGAG
    CTGAAAGCAG AGCGGAATAA CACGAGGTTG CTTCTGGAAC ATCTGGAGTG CCTGGTGTCC
    CGCCATGAGC GGTCACTGAG GATGACTGTG GTGAAGCGTC AGGCCCAGTC ACCCTCAGGG
    GTCTCCAGTG AGGTGGAGGT GCTGAAGGCT CTCAAGTCAC TGTTTGAGCA CCACAAGGCC
    CTGGATGAGA AGGTGCGAGA GCGGCTCCGG GTGGCCCTCG AGAGAGTGAC CACTTTGGAG
    GAACAGCTGG CGTGTGCCCA CCAGCAGGTG TCTTCCCTAC AGCAGGGGTC AGGGGTTCGG
    GATGGAGTGG CCGAAGAGGA GGGGACTGTG GAGCTGGGAT CAAAAGGCCT GTGGAAGGAG
    GACACAGGCC AGGTTGAGGA GCTGCAGGAG CTTCTGGAGA AGCAGAACTT TGAGTTGAGC
    CAGGCCCGGG AGCGACTGGT CACCCTGACA GCAACTGTGG CTGAACTGGA GGAGGACCTG
    GGCACAGCCC GCCGGGACCT CATCAAGTCG GAGGAACTGA GCAGCAAACA CCAGCGGGAC
    CTCCGGGAAG CTCTAGCCCA GAAAGAGGAC ATGGAGGAGC GGATCACCAC CCTGGAGAAG
    CGCTACCTGG CTGCTCAGCG TGAGGCTACA TCAATACATG ACCTCAATGA CAAACTGGAG
    AACGAGCTAG CCAATAGGGA ATCCTTGCAT CGCCAGTGTG AGGAGAAAGC CCGATATCTG
    CAGGAGCTAC TGGAGGTAGC CGAGCAGAAA CTTCAGCAGA CAATGCGCAA GGCCGAGACG
    CTGCCAGAGG TGGAGGCTGA GCTGGCCCAA AGAGTTGCGG CCCTCACCAA GGCTGAAGAG
    CGACATGGCA ACACTGAGGA GCACCTCCGA CAGCTGGAGG GACAGCTGGA GGAGAAGAAC
    CAAGAGCTGG CGCGGGTGCG CCAGCGGGAG AGGATGAATG AAGCCCACAA CAAGCGCCTG
    TCAGACACTG TGGACCGGCT GCTTATCGAG GCGAAGGAGC GCCTGCAGCT CCACCTCAGG
    GAGCGCATGG TGGCCCTGGA GGAGAAGGGC CGCCTGTCTG AAGAGATTGA GAAACTGCGC
    CAAGAGGTGG ACCAACTGAA GGGTCGAGGA GGACCATTTG TGGATGGCAT CCACTCCAGG
    GCGTACACGG GCAGTGCAAT GGATGTGCGG TTCTCCCTGA GCACAACTGC CCACACATCT
    CAAGGTCTGC GTCGTTGTTA CTCAGCACGG CGGGAACCGC CTGCCAAGGA CTGGGAGCCA
    TCTCCACGGC CTGCGGTGCT GGTCCCGACA ACCACCCCTG CCTTTGACAG TGACCGTGAG
    ATCTCTGATG TGGATGAGGA TGAACCAGGG GGTCTGGTGG GCTCCATGGA CGTTGTCTCC
    CCCAGCAGCC ACTCAGACGC CCAGACCCTG GCCATGATGC TGCAGGAGCA GCTGGATGCT
    ATCAACGAGG AGATCAGGAT GATCCAGGAA GAGAAGGAGT CCACAGAGCT CCGTGCTGAG
    GAGCTGGAAA CTCGAGTGAC TAGTGGCAGC ATGGAGGCCC TAGACCTGAC CCAGCTGCAC
    AAACGCGGTT CCATCCCCAC CTCTCTGACC GCCCTGTCCC TGGCCAGCGC GTCCCCTCCA
    CTTAGCGGCC GTGCCACACC TAAGCTCACC TCCCGCAGTG CTGCCCAGGA CCTGGATCGG
    ATGGGGGTCA TGACCTTGCC CAGTGACTTA AGAAAGCATA GGAGGAAGCT GCTGTCACCA
    GTGTCTTGGG AAGAGAACCG AGAGGATAAA GCCACCATAA AATGTGAGAC TTCCCCTCCT
    TCCTCACCCA GGACACTGCA GCTAGAGAAG CTTGGCCTCC CTGCCCTGAG CCAGGAAGAA
    GGCAAGAGTG CTTTGGAGGA TCCAGGCAGC AACCCCAGCA GCAGCAACAG CAGCCAGGAC
    TCCTTGCACA AGGGTGCCAA GCGCAAGGGC ATCAAATCAT CCATCGGCCG CCTGTTTGGG
    AAGAAGGAGA AAGGCAGGCT GATCCAGCCG AGCCGGGATG GACCCACAGG CCATGTTGTA
    CTACTAACAG ACTCTGAGCT CGGTCTGCAG GAGCCCATGG TGCCTGGCAA GCTGGGAACC
    CAGGCAGAAA AGGACCGGAG GCTGAAGAAG AAACACCAGC TGCTTGAAGA TGCCCGGAGA
    AAAGGAATGC CCTTTGCCCA GTGGGACGGC CCTACTGTGG TCTCCTGGCT AGAGCTCTGG
    GTGGGGATGC CTGCTTGGTA TGTGGCAGCC TGCCGGGCCA ACGTCAAGAG TGGCGCCATC
    ATGTCAGCCC TATCGGACAC AGAGATCCAG CGAGAAATTG GCATCAGCAA TGCCCTGCAC
    CGGCTCAAGC TCCGGCTGGC CATCCAGGAG ATGGTGTCGC TGACCAGCCC CTCTGCCCCG
    CCCACCTCCA GGACTTCTTC TGGGAATGTC TGGGTCACCC ACGAAGAGAT GGAAACTCTG
    GCAACGTCCA CTAAAACAGA CAGTGAGGAG GGCAGCTGGG CTCAGACCCT GGTCTATGGG
    GATATGAACC ACGAGTGGAT TGGGAACCAG TGGCTCCCCA GCCTGGGGCT CCCCCAGTAC
    CGCAGCTACT TCATGGAGTG CCTCGTGGAC GCTCGCATGC TGGATCACCT CACCAAGAAG
    GACTTGCGGG TCCACCTGAA GATGGTGGAC AGCTCCCACC GAACCAGTCT TCAGTATGGC
    ATCATGTGCC TGAAGAGGCT GAATTATGAC CGGAAGGAGC TGGAGAAGCG GCGGGAGGAG
    AGCCAGCATG AGATCAAGGA TGTGCTGGTC TGGACCAATG ACCAGGTAGT TCATTGGGTC
    CAGTCTATTG GGCTCCGAGA CTACGCAGGA AACCTCCATG AGAGTGGTGT GCATGGCGCT
    TTGCTGGCCC TGGATGAGAA CTTTGACCAC AACACACTGG CCCTGGTTCT CCAGATACCC
    ACACAAAATA CCCAGGCACG CCAAGTGTTG GAGAGAGAGT TCAACAACCT GTTGGCTTTG
    GGCACAGACC GGAAGCTGGA TGATGGGGAA GACAAGGCGC TACGCCGCGC GCCCTCCTGG
    AGGAAACGCT TCCGCCCGCG GGACCCCCAC GGCCGCGCCT CGCTCAGCGC CGCGGCCGAC
    ACGCTCCCGG CAACCTTCCG CGCGTCCACC GTGGGGCCCG TGCCGCCTCC AGCATCCCCG
    CGGAAGAAGA TCACGCCTGA AGGTCTGGAC CGCTCACTCC ACCGGAGACC AGGGTGCAGA
    ACCAAGGGCT GGGGTGGCCC TTCCAAAGAA AGGGCGCTTC AGACTTCCTT GCTTGGGTTA
    TTGGGCCTTC TGGTTTCTGT CCGTCTCTTG GTTGCACTAG ATGGTCTACG TCTGCTTTTG
    GCATCCCCCC TCCCGCCATT TTCTCTCCCC CAACTCATCC GCACCTTGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025133591.1
    CDS564..4394
    Translation

    Target ORF information:

    RefSeq Version XM_025277806.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis PTPRF interacting protein alpha 4 (PPFIA4), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025277806.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGAGGCAG GGCTCCCTTG GGCCCTAAAA CTTGACTGGC GTTGGGTAAA AATCTGGCTC 
    CCCGCCACCT CACGAGATAC TCTCCCGGCC GAGCCAAGAT GGCGCGCAGC CCCAAGTGGC
    TCCACGTCAC GAGGGAGGGA GCGCCGGGGC GGGGGCGTGG CCTCGAGGGG GCCGGCTAGC
    CCCGCCCTTG AGGTGGGGCC GGGGGCCGTC CGCAGAAGGC GAGGGCGGGG CGCTTCGGCT
    CGGGAAGGCT GCGGCTGCCT CGGCGCCGGC TCCGCTCGCG AGGACAAGTC CGGCGCCTCC
    AGGCCGGGCC GGCTTCCTGC GCAGAGCCAA GCTGCCCGGC CTTCGGAGTT TACCACCTTA
    ACCCGGGAGC TGAGTATGTG TCGGGAGCAG CTTCTAGAGA GGGAGGAAGA GATATCAGAG
    CTGAAAGCAG AGCGGAATAA CACGAGGTTG CTTCTGGAAC ATCTGGAGTG CCTGGTGTCC
    CGCCATGAGC GGTCACTGAG GATGACTGTG GTGAAGCGTC AGGCCCAGTC ACCCTCAGGG
    GTCTCCAGTG AGGTGGAGGT GCTGAAGGCT CTCAAGTCAC TGTTTGAGCA CCACAAGGCC
    CTGGATGAGA AGGTGCGAGA GCGGCTCCGG GTGGCCCTCG AGAGAGTGAC CACTTTGGAG
    GAACAGCTGG CGTGTGCCCA CCAGCAGGTG TCTTCCCTAC AGCAGGGGTC AGGGGTTCGG
    GATGGAGTGG CCGAAGAGGA GGGGACTGTG GAGCTGGGAT CAAAAGGCCT GTGGAAGGAG
    GACACAGGCC AGGTTGAGGA GCTGCAGGAG CTTCTGGAGA AGCAGAACTT TGAGTTGAGC
    CAGGCCCGGG AGCGACTGGT CACCCTGACA GCAACTGTGG CTGAACTGGA GGAGGACCTG
    GGCACAGCCC GCCGGGACCT CATCAAGTCG GAGGAACTGA GCAGCAAACA CCAGCGGGAC
    CTCCGGGAAG CTCTAGCCCA GAAAGAGGAC ATGGAGGAGC GGATCACCAC CCTGGAGAAG
    CGCTACCTGG CTGCTCAGCG TGAGGCTACA TCAATACATG ACCTCAATGA CAAACTGGAG
    AACGAGCTAG CCAATAGGGA ATCCTTGCAT CGCCAGTGTG AGGAGAAAGC CCGATATCTG
    CAGGAGCTAC TGGAGGTAGC CGAGCAGAAA CTTCAGCAGA CAATGCGCAA GGCCGAGACG
    CTGCCAGAGG TGGAGGCTGA GCTGGCCCAA AGAGTTGCGG CCCTCACCAA GGCTGAAGAG
    CGACATGGCA ACACTGAGGA GCACCTCCGA CAGCTGGAGG GACAGCTGGA GGAGAAGAAC
    CAAGAGCTGG CGCGGGTGCG CCAGCGGGAG AGGATGAATG AAGCCCACAA CAAGCGCCTG
    TCAGACACTG TGGACCGGCT GCTTATCGAG GCGAAGGAGC GCCTGCAGCT CCACCTCAGG
    GAGCGCATGG TGGCCCTGGA GGAGAAGGGC CGCCTGTCTG AAGAGATTGA GAAACTGCGC
    CAAGAGGTGG ACCAACTGAA GGGTCGAGGA GGACCATTTG TGGATGGCAT CCACTCCAGG
    GCGTACACGG GCAGTGCAAT GGATGTGCGG TTCTCCCTGA GCACAACTGC CCACACATCT
    CAAGGTCTGC GTCGTTGTTA CTCAGCACGG CGGGAACCGC CTGCCAAGGA CTGGGAGCCA
    TCTCCACGGC CTGCGGTGCT GGTCCCGACA ACCACCCCTG CCTTTGACAG TGACCGTGAG
    ATCTCTGATG TGGATGAGGA TGAACCAGGG GGTCTGGTGG GCTCCATGGA CGTTGTCTCC
    CCCAGCAGCC ACTCAGACGC CCAGACCCTG GCCATGATGC TGCAGGAGCA GCTGGATGCT
    ATCAACGAGG AGATCAGGAT GATCCAGGAA GAGAAGGAGT CCACAGAGCT CCGTGCTGAG
    GAGCTGGAAA CTCGAGTGAC TAGTGGCAGC ATGGAGGCCC TAGACCTGAC CCAGCTGCAC
    AAACGCGGTT CCATCCCCAC CTCTCTGACC GCCCTGTCCC TGGCCAGCGC GTCCCCTCCA
    CTTAGCGGCC GTGCCACACC TAAGCTCACC TCCCGCAGTG CTGCCCAGGA CCTGGATCGG
    ATGGGGGTCA TGACCTTGCC CAGTGACTTA AGAAAGCATA GGAGGAAGCT GCTGTCACCA
    GTGTCTTGGG AAGAGAACCG AGAGGATAAA GCCACCATAA AATGTGAGAC TTCCCCTCCT
    TCCTCACCCA GGACACTGCA GCTAGAGAAG CTTGGCCTCC CTGCCCTGAG CCAGGAAGAA
    GGCAAGAGTG CTTTGGAGGA TCCAGGCAGC AACCCCAGCA GCAGCAACAG CAGCCAGGAC
    TCCTTGCACA AGGGTGCCAA GCGCAAGGGC ATCAAATCAT CCATCGGCCG CCTGTTTGGG
    AAGAAGGAGA AAGGCAGGCT GATCCAGCCG AGCCGGGATG GACCCACAGG CCATGTTGTA
    CTACTAACAG ACTCTGAGCT CGGTCTGCAG GAGCCCATGG TGCCTGGCAA GCTGGGAACC
    CAGGCAGAAA AGGACCGGAG GCTGAAGAAG AAACACCAGC TGCTTGAAGA TGCCCGGAGA
    AAAGGAATGC CCTTTGCCCA GTGGGACGGC CCTACTGTGG TCTCCTGGCT AGAGCTCTGG
    GTGGGGATGC CTGCTTGGTA TGTGGCAGCC TGCCGGGCCA ACGTCAAGAG TGGCGCCATC
    ATGTCAGCCC TATCGGACAC AGAGATCCAG CGAGAAATTG GCATCAGCAA TGCCCTGCAC
    CGGCTCAAGC TCCGGCTGGC CATCCAGGAG ATGGTGTCGC TGACCAGCCC CTCTGCCCCG
    CCCACCTCCA GGACTTCTTC TGGGAATGTC TGGGTCACCC ACGAAGAGAT GGAAACTCTG
    GCAACGTCCA CTAAAACAGA CAGTGAGGAG GGCAGCTGGG CTCAGACCCT GGTCTATGGG
    GATATGAACC ACGAGTGGAT TGGGAACCAG TGGCTCCCCA GCCTGGGGCT CCCCCAGTAC
    CGCAGCTACT TCATGGAGTG CCTCGTGGAC GCTCGCATGC TGGATCACCT CACCAAGAAG
    GACTTGCGGG TCCACCTGAA GATGGTGGAC AGCTCCCACC GAACCAGTCT TCAGTATGGC
    ATCATGTGCC TGAAGAGGCT GAATTATGAC CGGAAGGAGC TGGAGAAGCG GCGGGAGGAG
    AGCCAGCATG AGATCAAGGA TGTGCTGGTC TGGACCAATG ACCAGGTAGT TCATTGGGTC
    CAGTCTATTG GGCTCCGAGA CTACGCAGGA AACCTCCATG AGAGTGGTGT GCATGGCGCT
    TTGCTGGCCC TGGATGAGAA CTTTGACCAC AACACACTGG CCCTGGTTCT CCAGATACCC
    ACACAAAATA CCCAGGCACG CCAAGTGTTG GAGAGAGAGT TCAACAACCT GTTGGCTTTG
    GGCACAGACC GGAAGCTGGA TGATGGGGAA GACAAGGCGC TACGCCGCGC GCCCTCCTGG
    AGGAAACGCT TCCGCCCGCG GGACCCCCAC GGCCGCGCCT CGCTCAGCGC CGCGGCCGAC
    ACGCTCCCGG CAACCTTCCG CGCGTCCACC GTGGGGCCCG TGCCGCCTCC AGCATCCCCG
    CGGAAGAAGA TCACGCCTGA AGGTCTGGAC CGCTCACTCC ACCGGAGACC AGGGTGCAGA
    ACCAAGGGCT GGGGTGGCCC TTCCAAAGAA AGGGCGCTTC AGACTTCCTT GCTTGGGTTA
    TTGGGCCTTC TGGTTTCTGT CCGTCTCTTG GTTGCACTAG ATGGTCTACG TCTGCTTTTG
    GCATCCCCCC TCCCGCCATT TTCTCTCCCC CAACTCATCC GCACCTTGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu41793
    Clone ID Related Accession (Same CDS sequence) XM_025277807.1
    Accession Version XM_025277807.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3804bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product liprin-alpha-4 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_020228958.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGAGGCAG GGCTCCCTTG GGCCCTAAAA CTTGACTGGC GTTGGGTAAA AATCTGGCTC 
    CCCGCCACCT CACGAGATAC TCTCCCGGCC GAGCCAAGAT GGCGCGCAGC CCCAAGTGGC
    TCCACGTCAC GAGGGAGGGA GCGCCGGGGC GGGGGCGTGG CCTCGAGGGG GCCGGCTAGC
    CCCGCCCTTG AGGTGGGGCC GGGGGCCGTC CGCAGAAGGC GAGGGCGGGG CGCTTCGGCT
    CGGGAAGGCT GCGGCTGCCT CGGCGCCGGC TCCGCTCGCG AGGACAAGTC CGGCGCCTCC
    AGGCCGGGCC GGCTTCCTGC GCAGAGCCAA GCTGCCCGGC CTTCGGAGTT TACCACCTTA
    ACCCGGGAGC TGAGTATGTG TCGGGAGCAG CTTCTAGAGA GGGAGGAAGA GATATCAGAG
    CTGAAAGCAG AGCGGAATAA CACGAGGTTG CTTCTGGAAC ATCTGGAGTG CCTGGTGTCC
    CGCCATGAGC GGTCACTGAG GATGACTGTG GTGAAGCGTC AGGCCCAGTC ACCCTCAGGG
    GTCTCCAGTG AGGTGGAGGT GCTGAAGGCT CTCAAGTCAC TGTTTGAGCA CCACAAGGCC
    CTGGATGAGA AGGTGCGAGA GCGGCTCCGG GTGGCCCTCG AGAGAGTGAC CACTTTGGAG
    GAACAGCTGG CGTGTGCCCA CCAGCAGGTG TCTTCCCTAC AGCAGGGGTC AGGGGTTCGG
    GATGGAGTGG CCGAAGAGGA GGGGACTGTG GAGCTGGGAT CAAAAGGCCT GTGGAAGGAG
    GACACAGGCC AGGTTGAGGA GCTGCAGGAG CTTCTGGAGA AGCAGAACTT TGAGTTGAGC
    CAGGCCCGGG AGCGACTGGT CACCCTGACA GCAACTGTGG CTGAACTGGA GGAGGACCTG
    GGCACAGCCC GCCGGGACCT CATCAAGTCG GAGGAACTGA GCAGCAAACA CCAGCGGGAC
    CTCCGGGAAG CTCTAGCCCA GAAAGAGGAC ATGGAGGAGC GGATCACCAC CCTGGAGAAG
    CGCTACCTGG CTGCTCAGCG TGAGGCTACA TCAATACATG ACCTCAATGA CAAACTGGAG
    AACGAGCTAG CCAATAGGGA ATCCTTGCAT CGCCAGTGTG AGGAGAAAGC CCGATATCTG
    CAGGAGCTAC TGGAGGTAGC CGAGCAGAAA CTTCAGCAGA CAATGCGCAA GGCCGAGACG
    CTGCCAGAGG TGGAGGCTGA GCTGGCCCAA AGAGTTGCGG CCCTCACCAA GGCTGAAGAG
    CGACATGGCA ACACTGAGGA GCACCTCCGA CAGCTGGAGG GACAGCTGGA GGAGAAGAAC
    CAAGAGCTGG CGCGGGTGCG CCAGCGGGAG AGGATGAATG AAGCCCACAA CAAGCGCCTG
    TCAGACACTG TGGACCGGCT GCTTATCGAG GCGAAGGAGC GCCTGCAGCT CCACCTCAGG
    GAGCGCATGG TGGCCCTGGA GGAGAAGAAC ACATTGATCC AAGAGCTGGA GACCTCCCAG
    CGGCAGATTG AAGAGCAGCA CCATCACAAG GGCCGCCTGT CTGAAGAGAT TGAGAAACTG
    CGCCAAGAGG TGGACCAACT GAAGGGTCGA GGAGGACCAT TTGTGGATGG CATCCACTCC
    AGGGCGTACA CGGGCAGTGC AATGGATGTG CGGTTCTCCC TGAGCACAAC TGCCCACACA
    TCTCAAGGTC TGCGTCGTTG TTACTCAGCA CGGCGGGAAC CGCCTGCCAA GGACTGGGAG
    CCATCTCCAC GGCCTGCGGT GCTGGTCCCG ACAACCACCC CTGCCTTTGA CAGTGACCGT
    GAGATCTCTG ATGTGGATGA GGATGAACCA GGGGGTCTGG TGGGCTCCAT GGACGTTGTC
    TCCCCCAGCA GCCACTCAGA CGCCCAGACC CTGGCCATGA TGCTGCAGGA GCAGCTGGAT
    GCTATCAACG AGGAGATCAG GATGATCCAG GAAGAGAAGG AGTCCACAGA GCTCCGTGCT
    GAGGAGCTGG AAACTCGAGT GACTAGTGGC AGCATGGAGG CCCTAGACCT GACCCAGCTG
    CACAAACGCG GTTCCATCCC CACCTCTCTG ACCGCCCTGT CCCTGGCCAG CGCGTCCCCT
    CCACTTAGCG GCCGTGCCAC ACCTAAGCTC ACCTCCCGCA GTGCTGCCCA GGACCTGGAT
    CGGATGGGGG TCATGACCTT GCCCAGTGAC TTAAGAAAGC ATAGGAGGAA GCTGCTGTCA
    CCAGTGTCTT GGGAAGAGAA CCGAGAGGAT AAAGCCACCA TAAAATGTGA GACTTCCCCT
    CCTTCCTCAC CCAGGACACT GCAGCTAGAG AAGCTTGGCC TCCCTGCCCT GAGCCAGGAA
    GAAGGCAAGA GTGCTTTGGA GGATCCAGGC AGCAACCCCA GCAGCAGCAA CAGCAGCCAG
    GACTCCTTGC ACAAGGGTGC CAAGCGCAAG GGCATCAAAT CATCCATCGG CCGCCTGTTT
    GGGAAGAAGG AGAAAGGCAG GCTGATCCAG CCGAGCCGGG ATGGACCCAC AGGCCATGTT
    GTACTACTAA CAGACTCTGA GCTCGGTCTG CAGGAGCCCA TGGTGCCTGG CAAGCTGGGA
    ACCCAGGCAG AAAAGGACCG GAGGCTGAAG AAGAAACACC AGCTGCTTGA AGATGCCCGG
    AGAAAAGGAA TGCCCTTTGC CCAGTGGGAC GGCCCTACTG TGGTCTCCTG GCTAGAGCTC
    TGGGTGGGGA TGCCTGCTTG GTATGTGGCA GCCTGCCGGG CCAACGTCAA GAGTGGCGCC
    ATCATGTCAG CCCTATCGGA CACAGAGATC CAGCGAGAAA TTGGCATCAG CAATGCCCTG
    CACCGGCTCA AGCTCCGGCT GGCCATCCAG GAGATGGTGT CGCTGACCAG CCCCTCTGCC
    CCGCCCACCT CCAGGACTAC CCTGGTCTAT GGGGATATGA ACCACGAGTG GATTGGGAAC
    CAGTGGCTCC CCAGCCTGGG GCTCCCCCAG TACCGCAGCT ACTTCATGGA GTGCCTCGTG
    GACGCTCGCA TGCTGGATCA CCTCACCAAG AAGGACTTGC GGGTCCACCT GAAGATGGTG
    GACAGCTCCC ACCGAACCAG TCTTCAGTAT GGCATCATGT GCCTGAAGAG GCTGAATTAT
    GACCGGAAGG AGCTGGAGAA GCGGCGGGAG GAGAGCCAGC ATGAGATCAA GGATGTGCTG
    GTCTGGACCA ATGACCAGGT AGTTCATTGG GTCCAGTCTA TTGGGCTCCG AGACTACGCA
    GGAAACCTCC ATGAGAGTGG TGTGCATGGC GCTTTGCTGG CCCTGGATGA GAACTTTGAC
    CACAACACAC TGGCCCTGGT TCTCCAGATA CCCACACAAA ATACCCAGGC ACGCCAAGTG
    TTGGAGAGAG AGTTCAACAA CCTGTTGGCT TTGGGCACAG ACCGGAAGCT GGATGATGGG
    GAAGACAAGG CGCTACGCCG CGCGCCCTCC TGGAGGAAAC GCTTCCGCCC GCGGGACCCC
    CACGGCCGCG CCTCGCTCAG CGCCGCGGCC GACACGCTCC CGGCAACCTT CCGCGCGTCC
    ACCGTGGGGC CCGTGCCGCC TCCAGCATCC CCGCGGAAGA AGATCACGCC TGAAGGTCTG
    GACCGCTCAC TCCACCGGAG ACCAGGGTGC AGAACCAAGG GCTGGGGTGG CCCTTCCAAA
    GAAAGGGCGC TTCAGACTTC CTTGCTTGGG TTATTGGGCC TTCTGGTTTC TGTCCGTCTC
    TTGGTTGCAC TAGATGGTCT ACGTCTGCTT TTGGCATCCC CCCTCCCGCC ATTTTCTCTC
    CCCCAACTCA TCCGCACCTT GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025133592.1
    CDS563..4366
    Translation

    Target ORF information:

    RefSeq Version XM_025277807.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis PTPRF interacting protein alpha 4 (PPFIA4), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025277807.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGAGGCAG GGCTCCCTTG GGCCCTAAAA CTTGACTGGC GTTGGGTAAA AATCTGGCTC 
    CCCGCCACCT CACGAGATAC TCTCCCGGCC GAGCCAAGAT GGCGCGCAGC CCCAAGTGGC
    TCCACGTCAC GAGGGAGGGA GCGCCGGGGC GGGGGCGTGG CCTCGAGGGG GCCGGCTAGC
    CCCGCCCTTG AGGTGGGGCC GGGGGCCGTC CGCAGAAGGC GAGGGCGGGG CGCTTCGGCT
    CGGGAAGGCT GCGGCTGCCT CGGCGCCGGC TCCGCTCGCG AGGACAAGTC CGGCGCCTCC
    AGGCCGGGCC GGCTTCCTGC GCAGAGCCAA GCTGCCCGGC CTTCGGAGTT TACCACCTTA
    ACCCGGGAGC TGAGTATGTG TCGGGAGCAG CTTCTAGAGA GGGAGGAAGA GATATCAGAG
    CTGAAAGCAG AGCGGAATAA CACGAGGTTG CTTCTGGAAC ATCTGGAGTG CCTGGTGTCC
    CGCCATGAGC GGTCACTGAG GATGACTGTG GTGAAGCGTC AGGCCCAGTC ACCCTCAGGG
    GTCTCCAGTG AGGTGGAGGT GCTGAAGGCT CTCAAGTCAC TGTTTGAGCA CCACAAGGCC
    CTGGATGAGA AGGTGCGAGA GCGGCTCCGG GTGGCCCTCG AGAGAGTGAC CACTTTGGAG
    GAACAGCTGG CGTGTGCCCA CCAGCAGGTG TCTTCCCTAC AGCAGGGGTC AGGGGTTCGG
    GATGGAGTGG CCGAAGAGGA GGGGACTGTG GAGCTGGGAT CAAAAGGCCT GTGGAAGGAG
    GACACAGGCC AGGTTGAGGA GCTGCAGGAG CTTCTGGAGA AGCAGAACTT TGAGTTGAGC
    CAGGCCCGGG AGCGACTGGT CACCCTGACA GCAACTGTGG CTGAACTGGA GGAGGACCTG
    GGCACAGCCC GCCGGGACCT CATCAAGTCG GAGGAACTGA GCAGCAAACA CCAGCGGGAC
    CTCCGGGAAG CTCTAGCCCA GAAAGAGGAC ATGGAGGAGC GGATCACCAC CCTGGAGAAG
    CGCTACCTGG CTGCTCAGCG TGAGGCTACA TCAATACATG ACCTCAATGA CAAACTGGAG
    AACGAGCTAG CCAATAGGGA ATCCTTGCAT CGCCAGTGTG AGGAGAAAGC CCGATATCTG
    CAGGAGCTAC TGGAGGTAGC CGAGCAGAAA CTTCAGCAGA CAATGCGCAA GGCCGAGACG
    CTGCCAGAGG TGGAGGCTGA GCTGGCCCAA AGAGTTGCGG CCCTCACCAA GGCTGAAGAG
    CGACATGGCA ACACTGAGGA GCACCTCCGA CAGCTGGAGG GACAGCTGGA GGAGAAGAAC
    CAAGAGCTGG CGCGGGTGCG CCAGCGGGAG AGGATGAATG AAGCCCACAA CAAGCGCCTG
    TCAGACACTG TGGACCGGCT GCTTATCGAG GCGAAGGAGC GCCTGCAGCT CCACCTCAGG
    GAGCGCATGG TGGCCCTGGA GGAGAAGAAC ACATTGATCC AAGAGCTGGA GACCTCCCAG
    CGGCAGATTG AAGAGCAGCA CCATCACAAG GGCCGCCTGT CTGAAGAGAT TGAGAAACTG
    CGCCAAGAGG TGGACCAACT GAAGGGTCGA GGAGGACCAT TTGTGGATGG CATCCACTCC
    AGGGCGTACA CGGGCAGTGC AATGGATGTG CGGTTCTCCC TGAGCACAAC TGCCCACACA
    TCTCAAGGTC TGCGTCGTTG TTACTCAGCA CGGCGGGAAC CGCCTGCCAA GGACTGGGAG
    CCATCTCCAC GGCCTGCGGT GCTGGTCCCG ACAACCACCC CTGCCTTTGA CAGTGACCGT
    GAGATCTCTG ATGTGGATGA GGATGAACCA GGGGGTCTGG TGGGCTCCAT GGACGTTGTC
    TCCCCCAGCA GCCACTCAGA CGCCCAGACC CTGGCCATGA TGCTGCAGGA GCAGCTGGAT
    GCTATCAACG AGGAGATCAG GATGATCCAG GAAGAGAAGG AGTCCACAGA GCTCCGTGCT
    GAGGAGCTGG AAACTCGAGT GACTAGTGGC AGCATGGAGG CCCTAGACCT GACCCAGCTG
    CACAAACGCG GTTCCATCCC CACCTCTCTG ACCGCCCTGT CCCTGGCCAG CGCGTCCCCT
    CCACTTAGCG GCCGTGCCAC ACCTAAGCTC ACCTCCCGCA GTGCTGCCCA GGACCTGGAT
    CGGATGGGGG TCATGACCTT GCCCAGTGAC TTAAGAAAGC ATAGGAGGAA GCTGCTGTCA
    CCAGTGTCTT GGGAAGAGAA CCGAGAGGAT AAAGCCACCA TAAAATGTGA GACTTCCCCT
    CCTTCCTCAC CCAGGACACT GCAGCTAGAG AAGCTTGGCC TCCCTGCCCT GAGCCAGGAA
    GAAGGCAAGA GTGCTTTGGA GGATCCAGGC AGCAACCCCA GCAGCAGCAA CAGCAGCCAG
    GACTCCTTGC ACAAGGGTGC CAAGCGCAAG GGCATCAAAT CATCCATCGG CCGCCTGTTT
    GGGAAGAAGG AGAAAGGCAG GCTGATCCAG CCGAGCCGGG ATGGACCCAC AGGCCATGTT
    GTACTACTAA CAGACTCTGA GCTCGGTCTG CAGGAGCCCA TGGTGCCTGG CAAGCTGGGA
    ACCCAGGCAG AAAAGGACCG GAGGCTGAAG AAGAAACACC AGCTGCTTGA AGATGCCCGG
    AGAAAAGGAA TGCCCTTTGC CCAGTGGGAC GGCCCTACTG TGGTCTCCTG GCTAGAGCTC
    TGGGTGGGGA TGCCTGCTTG GTATGTGGCA GCCTGCCGGG CCAACGTCAA GAGTGGCGCC
    ATCATGTCAG CCCTATCGGA CACAGAGATC CAGCGAGAAA TTGGCATCAG CAATGCCCTG
    CACCGGCTCA AGCTCCGGCT GGCCATCCAG GAGATGGTGT CGCTGACCAG CCCCTCTGCC
    CCGCCCACCT CCAGGACTAC CCTGGTCTAT GGGGATATGA ACCACGAGTG GATTGGGAAC
    CAGTGGCTCC CCAGCCTGGG GCTCCCCCAG TACCGCAGCT ACTTCATGGA GTGCCTCGTG
    GACGCTCGCA TGCTGGATCA CCTCACCAAG AAGGACTTGC GGGTCCACCT GAAGATGGTG
    GACAGCTCCC ACCGAACCAG TCTTCAGTAT GGCATCATGT GCCTGAAGAG GCTGAATTAT
    GACCGGAAGG AGCTGGAGAA GCGGCGGGAG GAGAGCCAGC ATGAGATCAA GGATGTGCTG
    GTCTGGACCA ATGACCAGGT AGTTCATTGG GTCCAGTCTA TTGGGCTCCG AGACTACGCA
    GGAAACCTCC ATGAGAGTGG TGTGCATGGC GCTTTGCTGG CCCTGGATGA GAACTTTGAC
    CACAACACAC TGGCCCTGGT TCTCCAGATA CCCACACAAA ATACCCAGGC ACGCCAAGTG
    TTGGAGAGAG AGTTCAACAA CCTGTTGGCT TTGGGCACAG ACCGGAAGCT GGATGATGGG
    GAAGACAAGG CGCTACGCCG CGCGCCCTCC TGGAGGAAAC GCTTCCGCCC GCGGGACCCC
    CACGGCCGCG CCTCGCTCAG CGCCGCGGCC GACACGCTCC CGGCAACCTT CCGCGCGTCC
    ACCGTGGGGC CCGTGCCGCC TCCAGCATCC CCGCGGAAGA AGATCACGCC TGAAGGTCTG
    GACCGCTCAC TCCACCGGAG ACCAGGGTGC AGAACCAAGG GCTGGGGTGG CCCTTCCAAA
    GAAAGGGCGC TTCAGACTTC CTTGCTTGGG TTATTGGGCC TTCTGGTTTC TGTCCGTCTC
    TTGGTTGCAC TAGATGGTCT ACGTCTGCTT TTGGCATCCC CCCTCCCGCC ATTTTCTCTC
    CCCCAACTCA TCCGCACCTT GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu41794
    Clone ID Related Accession (Same CDS sequence) XM_025277808.1 , XM_025277811.1 , XM_025277809.1 , XM_025277810.1
    Accession Version XM_025277808.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3783bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product liprin-alpha-4 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_020228958.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGTGTGAAG TGATGCCCAC AATCAACGAG GGAGACCCCC TGGGGCCTCC CCATGGAGCC 
    GATGCTGACG CCAACTTTGA GCAGCTGATG GTGAACATGC TGGATGAGCG GGAGAAGTTG
    CTAGAGTCCC TTCGGGAGAG TCAGGAGACT TTGCTGGCGA CACAGAGCCG GCTCCAGGAT
    GCCCTGCATG AGCGAGACCA GCTCCAGCGC CACCTTAACT CTGCCCTCCC CCAGGAGTTT
    ACCACCTTAA CCCGGGAGCT GAGTATGTGT CGGGAGCAGC TTCTAGAGAG GGAGGAAGAG
    ATATCAGAGC TGAAAGCAGA GCGGAATAAC ACGAGGTTGC TTCTGGAACA TCTGGAGTGC
    CTGGTGTCCC GCCATGAGCG GTCACTGAGG ATGACTGTGG TGAAGCGTCA GGCCCAGTCA
    CCCTCAGGGG TCTCCAGTGA GGTGGAGGTG CTGAAGGCTC TCAAGTCACT GTTTGAGCAC
    CACAAGGCCC TGGATGAGAA GGTGCGAGAG CGGCTCCGGG TGGCCCTCGA GAGAGTGACC
    ACTTTGGAGG AACAGCTGGC GTGTGCCCAC CAGCAGGTGT CTTCCCTACA GCAGGGGTCA
    GGGGTTCGGG ATGGAGTGGC CGAAGAGGAG GGGACTGTGG AGCTGGGATC AAAAGGCCTG
    TGGAAGGAGG ACACAGGCCA GGTTGAGGAG CTGCAGGAGC TTCTGGAGAA GCAGAACTTT
    GAGTTGAGCC AGGCCCGGGA GCGACTGGTC ACCCTGACAG CAACTGTGGC TGAACTGGAG
    GAGGACCTGG GCACAGCCCG CCGGGACCTC ATCAAGTCGG AGGAACTGAG CAGCAAACAC
    CAGCGGGACC TCCGGGAAGC TCTAGCCCAG AAAGAGGACA TGGAGGAGCG GATCACCACC
    CTGGAGAAGC GCTACCTGGC TGCTCAGCGT GAGGCTACAT CAATACATGA CCTCAATGAC
    AAACTGGAGA ACGAGCTAGC CAATAGGGAA TCCTTGCATC GCCAGTGTGA GGAGAAAGCC
    CGATATCTGC AGGAGCTACT GGAGGTAGCC GAGCAGAAAC TTCAGCAGAC AATGCGCAAG
    GCCGAGACGC TGCCAGAGGT GGAGGCTGAG CTGGCCCAAA GAGTTGCGGC CCTCACCAAG
    GCTGAAGAGC GACATGGCAA CACTGAGGAG CACCTCCGAC AGCTGGAGGG ACAGCTGGAG
    GAGAAGAACC AAGAGCTGGC GCGGGTGCGC CAGCGGGAGA GGATGAATGA AGCCCACAAC
    AAGCGCCTGT CAGACACTGT GGACCGGCTG CTTATCGAGG CGAAGGAGCG CCTGCAGCTC
    CACCTCAGGG AGCGCATGGT GGCCCTGGAG GAGAAGAACA CATTGATCCA AGAGCTGGAG
    ACCTCCCAGC GGCAGATTGA AGAGCAGCAC CATCACAAGG GCCGCCTGTC TGAAGAGATT
    GAGAAACTGC GCCAAGAGGT GGACCAACTG AAGGGTCGAG GAGGACCATT TGTGGATGGC
    ATCCACTCCA GGGCGTACAC GGGCAGTGCA ATGGATGTGC GGTTCTCCCT GAGCACAACT
    GCCCACACAT CTCAAGGTCT GCGTCGTTGT TACTCAGCAC GGCGGGAACC GCCTGCCAAG
    GACTGGGAGC CATCTCCACG GCCTGCGGTG CTGGTCCCGA CAACCACCCC TGCCTTTGAC
    AGTGACCGTG AGATCTCTGA TGTGGATGAG GATGAACCAG GGGGTCTGGT GGGCTCCATG
    GACGTTGTCT CCCCCAGCAG CCACTCAGAC GCCCAGACCC TGGCCATGAT GCTGCAGGAG
    CAGCTGGATG CTATCAACGA GGAGATCAGG ATGATCCAGG AAGAGAAGGA GTCCACAGAG
    CTCCGTGCTG AGGAGCTGGA AACTCGAGTG ACTAGTGGCA GCATGGAGGC CCTAGACCTG
    ACCCAGCTGC ACAAACGCGG TTCCATCCCC ACCTCTCTGA CCGCCCTGTC CCTGGCCAGC
    GCGTCCCCTC CACTTAGCGG CCGTGCCACA CCTAAGCTCA CCTCCCGCAG TGCTGCCCAG
    GACCTGGATC GGATGGGGGT CATGACCTTG CCCAGTGACT TAAGAAAGCA TAGGAGGAAG
    CTGCTGTCAC CAGTGTCTTG GGAAGAGAAC CGAGAGGATA AAGCCACCAT AAAATGTGAG
    ACTTCCCCTC CTTCCTCACC CAGGACACTG CAGCTAGAGA AGCTTGGCCT CCCTGCCCTG
    AGCCAGGAAG AAGGCAAGAG TGCTTTGGAG GATCCAGGCA GCAACCCCAG CAGCAGCAAC
    AGCAGCCAGG ACTCCTTGCA CAAGGGTGCC AAGCGCAAGG GCATCAAATC ATCCATCGGC
    CGCCTGTTTG GGAAGAAGGA GAAAGGCAGG CTGATCCAGC CGAGCCGGGA TGGACCCACA
    GGCCATGTTG TACTACTAAC AGACTCTGAG CTCGGTCTGC AGGAGCCCAT GGTGCCTGGC
    AAGCTGGGAA CCCAGGCAGA AAAGGACCGG AGGCTGAAGA AGAAACACCA GCTGCTTGAA
    GATGCCCGGA GAAAAGGAAT GCCCTTTGCC CAGTGGGACG GCCCTACTGT GGTCTCCTGG
    CTAGAGCTCT GGGTGGGGAT GCCTGCTTGG TATGTGGCAG CCTGCCGGGC CAACGTCAAG
    AGTGGCGCCA TCATGTCAGC CCTATCGGAC ACAGAGATCC AGCGAGAAAT TGGCATCAGC
    AATGCCCTGC ACCGGCTCAA GCTCCGGCTG GCCATCCAGG AGATGGTGTC GCTGACCAGC
    CCCTCTGCCC CGCCCACCTC CAGGACTTCT TCTGGGAATG TCTGGGTCAC CCACGAAGAG
    ATGGAAACTC TGGCAACGTC CACTAAAACA GACAGTGAGG AGGGCAGCTG GGCTCAGACC
    CTGGTCTATG GGGATATGAA CCACGAGTGG ATTGGGAACC AGTGGCTCCC CAGCCTGGGG
    CTCCCCCAGT ACCGCAGCTA CTTCATGGAG TGCCTCGTGG ACGCTCGCAT GCTGGATCAC
    CTCACCAAGA AGGACTTGCG GGTCCACCTG AAGATGGTGG ACAGCTCCCA CCGAACCAGT
    CTTCAGTATG GCATCATGTG CCTGAAGAGG CTGAATTATG ACCGGAAGGA GCTGGAGAAG
    CGGCGGGAGG AGAGCCAGCA TGAGATCAAG GATGTGCTGG TCTGGACCAA TGACCAGGTA
    GTTCATTGGG TCCAGTCTAT TGGGCTCCGA GACTACGCAG GAAACCTCCA TGAGAGTGGT
    GTGCATGGCG CTTTGCTGGC CCTGGATGAG AACTTTGACC ACAACACACT GGCCCTGGTT
    CTCCAGATAC CCACACAAAA TACCCAGGCA CGCCAAGTGT TGGAGAGAGA GTTCAACAAC
    CTGTTGGCTT TGGGCACAGA CCGGAAGCTG GATGATGGGG AAGACAAGGC GCTACGCCGC
    GCGCCCTCCT GGAGGAAACG CTTCCGCCCG CGGGACCCCC ACGGCCGCGC CTCGCTCAGC
    GCCGCGGCCG ACACGCTCCC GGCAACCTTC CGCGCGTCCA CCGTGGGGCC CGTGCCGCCT
    CCAGCATCCC CGCGGAAGAA GATCACGCCT GAAGGTCTGG ACCGCTCACT CCACCGGAGA
    CCAGGGTGCA GAACCAAGGG CTGGGGTGGC CCTTCCAAAG AAAGGGCGCT TCAGACTTCC
    TTGCTTGGGT TATTGGGCCT TCTGGTTTCT GTCCGTCTCT TGGTTGCACT AGATGGTCTA
    CGTCTGCTTT TGGCATCCCC CCTCCCGCCA TTTTCTCTCC CCCAACTCAT CCGCACCTTG
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025133593.1
    CDS487..4269
    Translation

    Target ORF information:

    RefSeq Version XM_025277808.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis PTPRF interacting protein alpha 4 (PPFIA4), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025277808.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGTGTGAAG TGATGCCCAC AATCAACGAG GGAGACCCCC TGGGGCCTCC CCATGGAGCC 
    GATGCTGACG CCAACTTTGA GCAGCTGATG GTGAACATGC TGGATGAGCG GGAGAAGTTG
    CTAGAGTCCC TTCGGGAGAG TCAGGAGACT TTGCTGGCGA CACAGAGCCG GCTCCAGGAT
    GCCCTGCATG AGCGAGACCA GCTCCAGCGC CACCTTAACT CTGCCCTCCC CCAGGAGTTT
    ACCACCTTAA CCCGGGAGCT GAGTATGTGT CGGGAGCAGC TTCTAGAGAG GGAGGAAGAG
    ATATCAGAGC TGAAAGCAGA GCGGAATAAC ACGAGGTTGC TTCTGGAACA TCTGGAGTGC
    CTGGTGTCCC GCCATGAGCG GTCACTGAGG ATGACTGTGG TGAAGCGTCA GGCCCAGTCA
    CCCTCAGGGG TCTCCAGTGA GGTGGAGGTG CTGAAGGCTC TCAAGTCACT GTTTGAGCAC
    CACAAGGCCC TGGATGAGAA GGTGCGAGAG CGGCTCCGGG TGGCCCTCGA GAGAGTGACC
    ACTTTGGAGG AACAGCTGGC GTGTGCCCAC CAGCAGGTGT CTTCCCTACA GCAGGGGTCA
    GGGGTTCGGG ATGGAGTGGC CGAAGAGGAG GGGACTGTGG AGCTGGGATC AAAAGGCCTG
    TGGAAGGAGG ACACAGGCCA GGTTGAGGAG CTGCAGGAGC TTCTGGAGAA GCAGAACTTT
    GAGTTGAGCC AGGCCCGGGA GCGACTGGTC ACCCTGACAG CAACTGTGGC TGAACTGGAG
    GAGGACCTGG GCACAGCCCG CCGGGACCTC ATCAAGTCGG AGGAACTGAG CAGCAAACAC
    CAGCGGGACC TCCGGGAAGC TCTAGCCCAG AAAGAGGACA TGGAGGAGCG GATCACCACC
    CTGGAGAAGC GCTACCTGGC TGCTCAGCGT GAGGCTACAT CAATACATGA CCTCAATGAC
    AAACTGGAGA ACGAGCTAGC CAATAGGGAA TCCTTGCATC GCCAGTGTGA GGAGAAAGCC
    CGATATCTGC AGGAGCTACT GGAGGTAGCC GAGCAGAAAC TTCAGCAGAC AATGCGCAAG
    GCCGAGACGC TGCCAGAGGT GGAGGCTGAG CTGGCCCAAA GAGTTGCGGC CCTCACCAAG
    GCTGAAGAGC GACATGGCAA CACTGAGGAG CACCTCCGAC AGCTGGAGGG ACAGCTGGAG
    GAGAAGAACC AAGAGCTGGC GCGGGTGCGC CAGCGGGAGA GGATGAATGA AGCCCACAAC
    AAGCGCCTGT CAGACACTGT GGACCGGCTG CTTATCGAGG CGAAGGAGCG CCTGCAGCTC
    CACCTCAGGG AGCGCATGGT GGCCCTGGAG GAGAAGAACA CATTGATCCA AGAGCTGGAG
    ACCTCCCAGC GGCAGATTGA AGAGCAGCAC CATCACAAGG GCCGCCTGTC TGAAGAGATT
    GAGAAACTGC GCCAAGAGGT GGACCAACTG AAGGGTCGAG GAGGACCATT TGTGGATGGC
    ATCCACTCCA GGGCGTACAC GGGCAGTGCA ATGGATGTGC GGTTCTCCCT GAGCACAACT
    GCCCACACAT CTCAAGGTCT GCGTCGTTGT TACTCAGCAC GGCGGGAACC GCCTGCCAAG
    GACTGGGAGC CATCTCCACG GCCTGCGGTG CTGGTCCCGA CAACCACCCC TGCCTTTGAC
    AGTGACCGTG AGATCTCTGA TGTGGATGAG GATGAACCAG GGGGTCTGGT GGGCTCCATG
    GACGTTGTCT CCCCCAGCAG CCACTCAGAC GCCCAGACCC TGGCCATGAT GCTGCAGGAG
    CAGCTGGATG CTATCAACGA GGAGATCAGG ATGATCCAGG AAGAGAAGGA GTCCACAGAG
    CTCCGTGCTG AGGAGCTGGA AACTCGAGTG ACTAGTGGCA GCATGGAGGC CCTAGACCTG
    ACCCAGCTGC ACAAACGCGG TTCCATCCCC ACCTCTCTGA CCGCCCTGTC CCTGGCCAGC
    GCGTCCCCTC CACTTAGCGG CCGTGCCACA CCTAAGCTCA CCTCCCGCAG TGCTGCCCAG
    GACCTGGATC GGATGGGGGT CATGACCTTG CCCAGTGACT TAAGAAAGCA TAGGAGGAAG
    CTGCTGTCAC CAGTGTCTTG GGAAGAGAAC CGAGAGGATA AAGCCACCAT AAAATGTGAG
    ACTTCCCCTC CTTCCTCACC CAGGACACTG CAGCTAGAGA AGCTTGGCCT CCCTGCCCTG
    AGCCAGGAAG AAGGCAAGAG TGCTTTGGAG GATCCAGGCA GCAACCCCAG CAGCAGCAAC
    AGCAGCCAGG ACTCCTTGCA CAAGGGTGCC AAGCGCAAGG GCATCAAATC ATCCATCGGC
    CGCCTGTTTG GGAAGAAGGA GAAAGGCAGG CTGATCCAGC CGAGCCGGGA TGGACCCACA
    GGCCATGTTG TACTACTAAC AGACTCTGAG CTCGGTCTGC AGGAGCCCAT GGTGCCTGGC
    AAGCTGGGAA CCCAGGCAGA AAAGGACCGG AGGCTGAAGA AGAAACACCA GCTGCTTGAA
    GATGCCCGGA GAAAAGGAAT GCCCTTTGCC CAGTGGGACG GCCCTACTGT GGTCTCCTGG
    CTAGAGCTCT GGGTGGGGAT GCCTGCTTGG TATGTGGCAG CCTGCCGGGC CAACGTCAAG
    AGTGGCGCCA TCATGTCAGC CCTATCGGAC ACAGAGATCC AGCGAGAAAT TGGCATCAGC
    AATGCCCTGC ACCGGCTCAA GCTCCGGCTG GCCATCCAGG AGATGGTGTC GCTGACCAGC
    CCCTCTGCCC CGCCCACCTC CAGGACTTCT TCTGGGAATG TCTGGGTCAC CCACGAAGAG
    ATGGAAACTC TGGCAACGTC CACTAAAACA GACAGTGAGG AGGGCAGCTG GGCTCAGACC
    CTGGTCTATG GGGATATGAA CCACGAGTGG ATTGGGAACC AGTGGCTCCC CAGCCTGGGG
    CTCCCCCAGT ACCGCAGCTA CTTCATGGAG TGCCTCGTGG ACGCTCGCAT GCTGGATCAC
    CTCACCAAGA AGGACTTGCG GGTCCACCTG AAGATGGTGG ACAGCTCCCA CCGAACCAGT
    CTTCAGTATG GCATCATGTG CCTGAAGAGG CTGAATTATG ACCGGAAGGA GCTGGAGAAG
    CGGCGGGAGG AGAGCCAGCA TGAGATCAAG GATGTGCTGG TCTGGACCAA TGACCAGGTA
    GTTCATTGGG TCCAGTCTAT TGGGCTCCGA GACTACGCAG GAAACCTCCA TGAGAGTGGT
    GTGCATGGCG CTTTGCTGGC CCTGGATGAG AACTTTGACC ACAACACACT GGCCCTGGTT
    CTCCAGATAC CCACACAAAA TACCCAGGCA CGCCAAGTGT TGGAGAGAGA GTTCAACAAC
    CTGTTGGCTT TGGGCACAGA CCGGAAGCTG GATGATGGGG AAGACAAGGC GCTACGCCGC
    GCGCCCTCCT GGAGGAAACG CTTCCGCCCG CGGGACCCCC ACGGCCGCGC CTCGCTCAGC
    GCCGCGGCCG ACACGCTCCC GGCAACCTTC CGCGCGTCCA CCGTGGGGCC CGTGCCGCCT
    CCAGCATCCC CGCGGAAGAA GATCACGCCT GAAGGTCTGG ACCGCTCACT CCACCGGAGA
    CCAGGGTGCA GAACCAAGGG CTGGGGTGGC CCTTCCAAAG AAAGGGCGCT TCAGACTTCC
    TTGCTTGGGT TATTGGGCCT TCTGGTTTCT GTCCGTCTCT TGGTTGCACT AGATGGTCTA
    CGTCTGCTTT TGGCATCCCC CCTCCCGCCA TTTTCTCTCC CCCAACTCAT CCGCACCTTG
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu41794
    Clone ID Related Accession (Same CDS sequence) XM_025277808.1 , XM_025277811.1 , XM_025277809.1 , XM_025277810.1
    Accession Version XM_025277811.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3783bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product liprin-alpha-4 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_020228958.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGTGTGAAG TGATGCCCAC AATCAACGAG GGAGACCCCC TGGGGCCTCC CCATGGAGCC 
    GATGCTGACG CCAACTTTGA GCAGCTGATG GTGAACATGC TGGATGAGCG GGAGAAGTTG
    CTAGAGTCCC TTCGGGAGAG TCAGGAGACT TTGCTGGCGA CACAGAGCCG GCTCCAGGAT
    GCCCTGCATG AGCGAGACCA GCTCCAGCGC CACCTTAACT CTGCCCTCCC CCAGGAGTTT
    ACCACCTTAA CCCGGGAGCT GAGTATGTGT CGGGAGCAGC TTCTAGAGAG GGAGGAAGAG
    ATATCAGAGC TGAAAGCAGA GCGGAATAAC ACGAGGTTGC TTCTGGAACA TCTGGAGTGC
    CTGGTGTCCC GCCATGAGCG GTCACTGAGG ATGACTGTGG TGAAGCGTCA GGCCCAGTCA
    CCCTCAGGGG TCTCCAGTGA GGTGGAGGTG CTGAAGGCTC TCAAGTCACT GTTTGAGCAC
    CACAAGGCCC TGGATGAGAA GGTGCGAGAG CGGCTCCGGG TGGCCCTCGA GAGAGTGACC
    ACTTTGGAGG AACAGCTGGC GTGTGCCCAC CAGCAGGTGT CTTCCCTACA GCAGGGGTCA
    GGGGTTCGGG ATGGAGTGGC CGAAGAGGAG GGGACTGTGG AGCTGGGATC AAAAGGCCTG
    TGGAAGGAGG ACACAGGCCA GGTTGAGGAG CTGCAGGAGC TTCTGGAGAA GCAGAACTTT
    GAGTTGAGCC AGGCCCGGGA GCGACTGGTC ACCCTGACAG CAACTGTGGC TGAACTGGAG
    GAGGACCTGG GCACAGCCCG CCGGGACCTC ATCAAGTCGG AGGAACTGAG CAGCAAACAC
    CAGCGGGACC TCCGGGAAGC TCTAGCCCAG AAAGAGGACA TGGAGGAGCG GATCACCACC
    CTGGAGAAGC GCTACCTGGC TGCTCAGCGT GAGGCTACAT CAATACATGA CCTCAATGAC
    AAACTGGAGA ACGAGCTAGC CAATAGGGAA TCCTTGCATC GCCAGTGTGA GGAGAAAGCC
    CGATATCTGC AGGAGCTACT GGAGGTAGCC GAGCAGAAAC TTCAGCAGAC AATGCGCAAG
    GCCGAGACGC TGCCAGAGGT GGAGGCTGAG CTGGCCCAAA GAGTTGCGGC CCTCACCAAG
    GCTGAAGAGC GACATGGCAA CACTGAGGAG CACCTCCGAC AGCTGGAGGG ACAGCTGGAG
    GAGAAGAACC AAGAGCTGGC GCGGGTGCGC CAGCGGGAGA GGATGAATGA AGCCCACAAC
    AAGCGCCTGT CAGACACTGT GGACCGGCTG CTTATCGAGG CGAAGGAGCG CCTGCAGCTC
    CACCTCAGGG AGCGCATGGT GGCCCTGGAG GAGAAGAACA CATTGATCCA AGAGCTGGAG
    ACCTCCCAGC GGCAGATTGA AGAGCAGCAC CATCACAAGG GCCGCCTGTC TGAAGAGATT
    GAGAAACTGC GCCAAGAGGT GGACCAACTG AAGGGTCGAG GAGGACCATT TGTGGATGGC
    ATCCACTCCA GGGCGTACAC GGGCAGTGCA ATGGATGTGC GGTTCTCCCT GAGCACAACT
    GCCCACACAT CTCAAGGTCT GCGTCGTTGT TACTCAGCAC GGCGGGAACC GCCTGCCAAG
    GACTGGGAGC CATCTCCACG GCCTGCGGTG CTGGTCCCGA CAACCACCCC TGCCTTTGAC
    AGTGACCGTG AGATCTCTGA TGTGGATGAG GATGAACCAG GGGGTCTGGT GGGCTCCATG
    GACGTTGTCT CCCCCAGCAG CCACTCAGAC GCCCAGACCC TGGCCATGAT GCTGCAGGAG
    CAGCTGGATG CTATCAACGA GGAGATCAGG ATGATCCAGG AAGAGAAGGA GTCCACAGAG
    CTCCGTGCTG AGGAGCTGGA AACTCGAGTG ACTAGTGGCA GCATGGAGGC CCTAGACCTG
    ACCCAGCTGC ACAAACGCGG TTCCATCCCC ACCTCTCTGA CCGCCCTGTC CCTGGCCAGC
    GCGTCCCCTC CACTTAGCGG CCGTGCCACA CCTAAGCTCA CCTCCCGCAG TGCTGCCCAG
    GACCTGGATC GGATGGGGGT CATGACCTTG CCCAGTGACT TAAGAAAGCA TAGGAGGAAG
    CTGCTGTCAC CAGTGTCTTG GGAAGAGAAC CGAGAGGATA AAGCCACCAT AAAATGTGAG
    ACTTCCCCTC CTTCCTCACC CAGGACACTG CAGCTAGAGA AGCTTGGCCT CCCTGCCCTG
    AGCCAGGAAG AAGGCAAGAG TGCTTTGGAG GATCCAGGCA GCAACCCCAG CAGCAGCAAC
    AGCAGCCAGG ACTCCTTGCA CAAGGGTGCC AAGCGCAAGG GCATCAAATC ATCCATCGGC
    CGCCTGTTTG GGAAGAAGGA GAAAGGCAGG CTGATCCAGC CGAGCCGGGA TGGACCCACA
    GGCCATGTTG TACTACTAAC AGACTCTGAG CTCGGTCTGC AGGAGCCCAT GGTGCCTGGC
    AAGCTGGGAA CCCAGGCAGA AAAGGACCGG AGGCTGAAGA AGAAACACCA GCTGCTTGAA
    GATGCCCGGA GAAAAGGAAT GCCCTTTGCC CAGTGGGACG GCCCTACTGT GGTCTCCTGG
    CTAGAGCTCT GGGTGGGGAT GCCTGCTTGG TATGTGGCAG CCTGCCGGGC CAACGTCAAG
    AGTGGCGCCA TCATGTCAGC CCTATCGGAC ACAGAGATCC AGCGAGAAAT TGGCATCAGC
    AATGCCCTGC ACCGGCTCAA GCTCCGGCTG GCCATCCAGG AGATGGTGTC GCTGACCAGC
    CCCTCTGCCC CGCCCACCTC CAGGACTTCT TCTGGGAATG TCTGGGTCAC CCACGAAGAG
    ATGGAAACTC TGGCAACGTC CACTAAAACA GACAGTGAGG AGGGCAGCTG GGCTCAGACC
    CTGGTCTATG GGGATATGAA CCACGAGTGG ATTGGGAACC AGTGGCTCCC CAGCCTGGGG
    CTCCCCCAGT ACCGCAGCTA CTTCATGGAG TGCCTCGTGG ACGCTCGCAT GCTGGATCAC
    CTCACCAAGA AGGACTTGCG GGTCCACCTG AAGATGGTGG ACAGCTCCCA CCGAACCAGT
    CTTCAGTATG GCATCATGTG CCTGAAGAGG CTGAATTATG ACCGGAAGGA GCTGGAGAAG
    CGGCGGGAGG AGAGCCAGCA TGAGATCAAG GATGTGCTGG TCTGGACCAA TGACCAGGTA
    GTTCATTGGG TCCAGTCTAT TGGGCTCCGA GACTACGCAG GAAACCTCCA TGAGAGTGGT
    GTGCATGGCG CTTTGCTGGC CCTGGATGAG AACTTTGACC ACAACACACT GGCCCTGGTT
    CTCCAGATAC CCACACAAAA TACCCAGGCA CGCCAAGTGT TGGAGAGAGA GTTCAACAAC
    CTGTTGGCTT TGGGCACAGA CCGGAAGCTG GATGATGGGG AAGACAAGGC GCTACGCCGC
    GCGCCCTCCT GGAGGAAACG CTTCCGCCCG CGGGACCCCC ACGGCCGCGC CTCGCTCAGC
    GCCGCGGCCG ACACGCTCCC GGCAACCTTC CGCGCGTCCA CCGTGGGGCC CGTGCCGCCT
    CCAGCATCCC CGCGGAAGAA GATCACGCCT GAAGGTCTGG ACCGCTCACT CCACCGGAGA
    CCAGGGTGCA GAACCAAGGG CTGGGGTGGC CCTTCCAAAG AAAGGGCGCT TCAGACTTCC
    TTGCTTGGGT TATTGGGCCT TCTGGTTTCT GTCCGTCTCT TGGTTGCACT AGATGGTCTA
    CGTCTGCTTT TGGCATCCCC CCTCCCGCCA TTTTCTCTCC CCCAACTCAT CCGCACCTTG
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025133596.1
    CDS285..4067
    Translation

    Target ORF information:

    RefSeq Version XM_025277811.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis PTPRF interacting protein alpha 4 (PPFIA4), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025277811.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGTGTGAAG TGATGCCCAC AATCAACGAG GGAGACCCCC TGGGGCCTCC CCATGGAGCC 
    GATGCTGACG CCAACTTTGA GCAGCTGATG GTGAACATGC TGGATGAGCG GGAGAAGTTG
    CTAGAGTCCC TTCGGGAGAG TCAGGAGACT TTGCTGGCGA CACAGAGCCG GCTCCAGGAT
    GCCCTGCATG AGCGAGACCA GCTCCAGCGC CACCTTAACT CTGCCCTCCC CCAGGAGTTT
    ACCACCTTAA CCCGGGAGCT GAGTATGTGT CGGGAGCAGC TTCTAGAGAG GGAGGAAGAG
    ATATCAGAGC TGAAAGCAGA GCGGAATAAC ACGAGGTTGC TTCTGGAACA TCTGGAGTGC
    CTGGTGTCCC GCCATGAGCG GTCACTGAGG ATGACTGTGG TGAAGCGTCA GGCCCAGTCA
    CCCTCAGGGG TCTCCAGTGA GGTGGAGGTG CTGAAGGCTC TCAAGTCACT GTTTGAGCAC
    CACAAGGCCC TGGATGAGAA GGTGCGAGAG CGGCTCCGGG TGGCCCTCGA GAGAGTGACC
    ACTTTGGAGG AACAGCTGGC GTGTGCCCAC CAGCAGGTGT CTTCCCTACA GCAGGGGTCA
    GGGGTTCGGG ATGGAGTGGC CGAAGAGGAG GGGACTGTGG AGCTGGGATC AAAAGGCCTG
    TGGAAGGAGG ACACAGGCCA GGTTGAGGAG CTGCAGGAGC TTCTGGAGAA GCAGAACTTT
    GAGTTGAGCC AGGCCCGGGA GCGACTGGTC ACCCTGACAG CAACTGTGGC TGAACTGGAG
    GAGGACCTGG GCACAGCCCG CCGGGACCTC ATCAAGTCGG AGGAACTGAG CAGCAAACAC
    CAGCGGGACC TCCGGGAAGC TCTAGCCCAG AAAGAGGACA TGGAGGAGCG GATCACCACC
    CTGGAGAAGC GCTACCTGGC TGCTCAGCGT GAGGCTACAT CAATACATGA CCTCAATGAC
    AAACTGGAGA ACGAGCTAGC CAATAGGGAA TCCTTGCATC GCCAGTGTGA GGAGAAAGCC
    CGATATCTGC AGGAGCTACT GGAGGTAGCC GAGCAGAAAC TTCAGCAGAC AATGCGCAAG
    GCCGAGACGC TGCCAGAGGT GGAGGCTGAG CTGGCCCAAA GAGTTGCGGC CCTCACCAAG
    GCTGAAGAGC GACATGGCAA CACTGAGGAG CACCTCCGAC AGCTGGAGGG ACAGCTGGAG
    GAGAAGAACC AAGAGCTGGC GCGGGTGCGC CAGCGGGAGA GGATGAATGA AGCCCACAAC
    AAGCGCCTGT CAGACACTGT GGACCGGCTG CTTATCGAGG CGAAGGAGCG CCTGCAGCTC
    CACCTCAGGG AGCGCATGGT GGCCCTGGAG GAGAAGAACA CATTGATCCA AGAGCTGGAG
    ACCTCCCAGC GGCAGATTGA AGAGCAGCAC CATCACAAGG GCCGCCTGTC TGAAGAGATT
    GAGAAACTGC GCCAAGAGGT GGACCAACTG AAGGGTCGAG GAGGACCATT TGTGGATGGC
    ATCCACTCCA GGGCGTACAC GGGCAGTGCA ATGGATGTGC GGTTCTCCCT GAGCACAACT
    GCCCACACAT CTCAAGGTCT GCGTCGTTGT TACTCAGCAC GGCGGGAACC GCCTGCCAAG
    GACTGGGAGC CATCTCCACG GCCTGCGGTG CTGGTCCCGA CAACCACCCC TGCCTTTGAC
    AGTGACCGTG AGATCTCTGA TGTGGATGAG GATGAACCAG GGGGTCTGGT GGGCTCCATG
    GACGTTGTCT CCCCCAGCAG CCACTCAGAC GCCCAGACCC TGGCCATGAT GCTGCAGGAG
    CAGCTGGATG CTATCAACGA GGAGATCAGG ATGATCCAGG AAGAGAAGGA GTCCACAGAG
    CTCCGTGCTG AGGAGCTGGA AACTCGAGTG ACTAGTGGCA GCATGGAGGC CCTAGACCTG
    ACCCAGCTGC ACAAACGCGG TTCCATCCCC ACCTCTCTGA CCGCCCTGTC CCTGGCCAGC
    GCGTCCCCTC CACTTAGCGG CCGTGCCACA CCTAAGCTCA CCTCCCGCAG TGCTGCCCAG
    GACCTGGATC GGATGGGGGT CATGACCTTG CCCAGTGACT TAAGAAAGCA TAGGAGGAAG
    CTGCTGTCAC CAGTGTCTTG GGAAGAGAAC CGAGAGGATA AAGCCACCAT AAAATGTGAG
    ACTTCCCCTC CTTCCTCACC CAGGACACTG CAGCTAGAGA AGCTTGGCCT CCCTGCCCTG
    AGCCAGGAAG AAGGCAAGAG TGCTTTGGAG GATCCAGGCA GCAACCCCAG CAGCAGCAAC
    AGCAGCCAGG ACTCCTTGCA CAAGGGTGCC AAGCGCAAGG GCATCAAATC ATCCATCGGC
    CGCCTGTTTG GGAAGAAGGA GAAAGGCAGG CTGATCCAGC CGAGCCGGGA TGGACCCACA
    GGCCATGTTG TACTACTAAC AGACTCTGAG CTCGGTCTGC AGGAGCCCAT GGTGCCTGGC
    AAGCTGGGAA CCCAGGCAGA AAAGGACCGG AGGCTGAAGA AGAAACACCA GCTGCTTGAA
    GATGCCCGGA GAAAAGGAAT GCCCTTTGCC CAGTGGGACG GCCCTACTGT GGTCTCCTGG
    CTAGAGCTCT GGGTGGGGAT GCCTGCTTGG TATGTGGCAG CCTGCCGGGC CAACGTCAAG
    AGTGGCGCCA TCATGTCAGC CCTATCGGAC ACAGAGATCC AGCGAGAAAT TGGCATCAGC
    AATGCCCTGC ACCGGCTCAA GCTCCGGCTG GCCATCCAGG AGATGGTGTC GCTGACCAGC
    CCCTCTGCCC CGCCCACCTC CAGGACTTCT TCTGGGAATG TCTGGGTCAC CCACGAAGAG
    ATGGAAACTC TGGCAACGTC CACTAAAACA GACAGTGAGG AGGGCAGCTG GGCTCAGACC
    CTGGTCTATG GGGATATGAA CCACGAGTGG ATTGGGAACC AGTGGCTCCC CAGCCTGGGG
    CTCCCCCAGT ACCGCAGCTA CTTCATGGAG TGCCTCGTGG ACGCTCGCAT GCTGGATCAC
    CTCACCAAGA AGGACTTGCG GGTCCACCTG AAGATGGTGG ACAGCTCCCA CCGAACCAGT
    CTTCAGTATG GCATCATGTG CCTGAAGAGG CTGAATTATG ACCGGAAGGA GCTGGAGAAG
    CGGCGGGAGG AGAGCCAGCA TGAGATCAAG GATGTGCTGG TCTGGACCAA TGACCAGGTA
    GTTCATTGGG TCCAGTCTAT TGGGCTCCGA GACTACGCAG GAAACCTCCA TGAGAGTGGT
    GTGCATGGCG CTTTGCTGGC CCTGGATGAG AACTTTGACC ACAACACACT GGCCCTGGTT
    CTCCAGATAC CCACACAAAA TACCCAGGCA CGCCAAGTGT TGGAGAGAGA GTTCAACAAC
    CTGTTGGCTT TGGGCACAGA CCGGAAGCTG GATGATGGGG AAGACAAGGC GCTACGCCGC
    GCGCCCTCCT GGAGGAAACG CTTCCGCCCG CGGGACCCCC ACGGCCGCGC CTCGCTCAGC
    GCCGCGGCCG ACACGCTCCC GGCAACCTTC CGCGCGTCCA CCGTGGGGCC CGTGCCGCCT
    CCAGCATCCC CGCGGAAGAA GATCACGCCT GAAGGTCTGG ACCGCTCACT CCACCGGAGA
    CCAGGGTGCA GAACCAAGGG CTGGGGTGGC CCTTCCAAAG AAAGGGCGCT TCAGACTTCC
    TTGCTTGGGT TATTGGGCCT TCTGGTTTCT GTCCGTCTCT TGGTTGCACT AGATGGTCTA
    CGTCTGCTTT TGGCATCCCC CCTCCCGCCA TTTTCTCTCC CCCAACTCAT CCGCACCTTG
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu41794
    Clone ID Related Accession (Same CDS sequence) XM_025277808.1 , XM_025277811.1 , XM_025277809.1 , XM_025277810.1
    Accession Version XM_025277809.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3783bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product liprin-alpha-4 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_020228958.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGTGTGAAG TGATGCCCAC AATCAACGAG GGAGACCCCC TGGGGCCTCC CCATGGAGCC 
    GATGCTGACG CCAACTTTGA GCAGCTGATG GTGAACATGC TGGATGAGCG GGAGAAGTTG
    CTAGAGTCCC TTCGGGAGAG TCAGGAGACT TTGCTGGCGA CACAGAGCCG GCTCCAGGAT
    GCCCTGCATG AGCGAGACCA GCTCCAGCGC CACCTTAACT CTGCCCTCCC CCAGGAGTTT
    ACCACCTTAA CCCGGGAGCT GAGTATGTGT CGGGAGCAGC TTCTAGAGAG GGAGGAAGAG
    ATATCAGAGC TGAAAGCAGA GCGGAATAAC ACGAGGTTGC TTCTGGAACA TCTGGAGTGC
    CTGGTGTCCC GCCATGAGCG GTCACTGAGG ATGACTGTGG TGAAGCGTCA GGCCCAGTCA
    CCCTCAGGGG TCTCCAGTGA GGTGGAGGTG CTGAAGGCTC TCAAGTCACT GTTTGAGCAC
    CACAAGGCCC TGGATGAGAA GGTGCGAGAG CGGCTCCGGG TGGCCCTCGA GAGAGTGACC
    ACTTTGGAGG AACAGCTGGC GTGTGCCCAC CAGCAGGTGT CTTCCCTACA GCAGGGGTCA
    GGGGTTCGGG ATGGAGTGGC CGAAGAGGAG GGGACTGTGG AGCTGGGATC AAAAGGCCTG
    TGGAAGGAGG ACACAGGCCA GGTTGAGGAG CTGCAGGAGC TTCTGGAGAA GCAGAACTTT
    GAGTTGAGCC AGGCCCGGGA GCGACTGGTC ACCCTGACAG CAACTGTGGC TGAACTGGAG
    GAGGACCTGG GCACAGCCCG CCGGGACCTC ATCAAGTCGG AGGAACTGAG CAGCAAACAC
    CAGCGGGACC TCCGGGAAGC TCTAGCCCAG AAAGAGGACA TGGAGGAGCG GATCACCACC
    CTGGAGAAGC GCTACCTGGC TGCTCAGCGT GAGGCTACAT CAATACATGA CCTCAATGAC
    AAACTGGAGA ACGAGCTAGC CAATAGGGAA TCCTTGCATC GCCAGTGTGA GGAGAAAGCC
    CGATATCTGC AGGAGCTACT GGAGGTAGCC GAGCAGAAAC TTCAGCAGAC AATGCGCAAG
    GCCGAGACGC TGCCAGAGGT GGAGGCTGAG CTGGCCCAAA GAGTTGCGGC CCTCACCAAG
    GCTGAAGAGC GACATGGCAA CACTGAGGAG CACCTCCGAC AGCTGGAGGG ACAGCTGGAG
    GAGAAGAACC AAGAGCTGGC GCGGGTGCGC CAGCGGGAGA GGATGAATGA AGCCCACAAC
    AAGCGCCTGT CAGACACTGT GGACCGGCTG CTTATCGAGG CGAAGGAGCG CCTGCAGCTC
    CACCTCAGGG AGCGCATGGT GGCCCTGGAG GAGAAGAACA CATTGATCCA AGAGCTGGAG
    ACCTCCCAGC GGCAGATTGA AGAGCAGCAC CATCACAAGG GCCGCCTGTC TGAAGAGATT
    GAGAAACTGC GCCAAGAGGT GGACCAACTG AAGGGTCGAG GAGGACCATT TGTGGATGGC
    ATCCACTCCA GGGCGTACAC GGGCAGTGCA ATGGATGTGC GGTTCTCCCT GAGCACAACT
    GCCCACACAT CTCAAGGTCT GCGTCGTTGT TACTCAGCAC GGCGGGAACC GCCTGCCAAG
    GACTGGGAGC CATCTCCACG GCCTGCGGTG CTGGTCCCGA CAACCACCCC TGCCTTTGAC
    AGTGACCGTG AGATCTCTGA TGTGGATGAG GATGAACCAG GGGGTCTGGT GGGCTCCATG
    GACGTTGTCT CCCCCAGCAG CCACTCAGAC GCCCAGACCC TGGCCATGAT GCTGCAGGAG
    CAGCTGGATG CTATCAACGA GGAGATCAGG ATGATCCAGG AAGAGAAGGA GTCCACAGAG
    CTCCGTGCTG AGGAGCTGGA AACTCGAGTG ACTAGTGGCA GCATGGAGGC CCTAGACCTG
    ACCCAGCTGC ACAAACGCGG TTCCATCCCC ACCTCTCTGA CCGCCCTGTC CCTGGCCAGC
    GCGTCCCCTC CACTTAGCGG CCGTGCCACA CCTAAGCTCA CCTCCCGCAG TGCTGCCCAG
    GACCTGGATC GGATGGGGGT CATGACCTTG CCCAGTGACT TAAGAAAGCA TAGGAGGAAG
    CTGCTGTCAC CAGTGTCTTG GGAAGAGAAC CGAGAGGATA AAGCCACCAT AAAATGTGAG
    ACTTCCCCTC CTTCCTCACC CAGGACACTG CAGCTAGAGA AGCTTGGCCT CCCTGCCCTG
    AGCCAGGAAG AAGGCAAGAG TGCTTTGGAG GATCCAGGCA GCAACCCCAG CAGCAGCAAC
    AGCAGCCAGG ACTCCTTGCA CAAGGGTGCC AAGCGCAAGG GCATCAAATC ATCCATCGGC
    CGCCTGTTTG GGAAGAAGGA GAAAGGCAGG CTGATCCAGC CGAGCCGGGA TGGACCCACA
    GGCCATGTTG TACTACTAAC AGACTCTGAG CTCGGTCTGC AGGAGCCCAT GGTGCCTGGC
    AAGCTGGGAA CCCAGGCAGA AAAGGACCGG AGGCTGAAGA AGAAACACCA GCTGCTTGAA
    GATGCCCGGA GAAAAGGAAT GCCCTTTGCC CAGTGGGACG GCCCTACTGT GGTCTCCTGG
    CTAGAGCTCT GGGTGGGGAT GCCTGCTTGG TATGTGGCAG CCTGCCGGGC CAACGTCAAG
    AGTGGCGCCA TCATGTCAGC CCTATCGGAC ACAGAGATCC AGCGAGAAAT TGGCATCAGC
    AATGCCCTGC ACCGGCTCAA GCTCCGGCTG GCCATCCAGG AGATGGTGTC GCTGACCAGC
    CCCTCTGCCC CGCCCACCTC CAGGACTTCT TCTGGGAATG TCTGGGTCAC CCACGAAGAG
    ATGGAAACTC TGGCAACGTC CACTAAAACA GACAGTGAGG AGGGCAGCTG GGCTCAGACC
    CTGGTCTATG GGGATATGAA CCACGAGTGG ATTGGGAACC AGTGGCTCCC CAGCCTGGGG
    CTCCCCCAGT ACCGCAGCTA CTTCATGGAG TGCCTCGTGG ACGCTCGCAT GCTGGATCAC
    CTCACCAAGA AGGACTTGCG GGTCCACCTG AAGATGGTGG ACAGCTCCCA CCGAACCAGT
    CTTCAGTATG GCATCATGTG CCTGAAGAGG CTGAATTATG ACCGGAAGGA GCTGGAGAAG
    CGGCGGGAGG AGAGCCAGCA TGAGATCAAG GATGTGCTGG TCTGGACCAA TGACCAGGTA
    GTTCATTGGG TCCAGTCTAT TGGGCTCCGA GACTACGCAG GAAACCTCCA TGAGAGTGGT
    GTGCATGGCG CTTTGCTGGC CCTGGATGAG AACTTTGACC ACAACACACT GGCCCTGGTT
    CTCCAGATAC CCACACAAAA TACCCAGGCA CGCCAAGTGT TGGAGAGAGA GTTCAACAAC
    CTGTTGGCTT TGGGCACAGA CCGGAAGCTG GATGATGGGG AAGACAAGGC GCTACGCCGC
    GCGCCCTCCT GGAGGAAACG CTTCCGCCCG CGGGACCCCC ACGGCCGCGC CTCGCTCAGC
    GCCGCGGCCG ACACGCTCCC GGCAACCTTC CGCGCGTCCA CCGTGGGGCC CGTGCCGCCT
    CCAGCATCCC CGCGGAAGAA GATCACGCCT GAAGGTCTGG ACCGCTCACT CCACCGGAGA
    CCAGGGTGCA GAACCAAGGG CTGGGGTGGC CCTTCCAAAG AAAGGGCGCT TCAGACTTCC
    TTGCTTGGGT TATTGGGCCT TCTGGTTTCT GTCCGTCTCT TGGTTGCACT AGATGGTCTA
    CGTCTGCTTT TGGCATCCCC CCTCCCGCCA TTTTCTCTCC CCCAACTCAT CCGCACCTTG
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025133594.1
    CDS488..4270
    Translation

    Target ORF information:

    RefSeq Version XM_025277809.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis PTPRF interacting protein alpha 4 (PPFIA4), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025277809.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGTGTGAAG TGATGCCCAC AATCAACGAG GGAGACCCCC TGGGGCCTCC CCATGGAGCC 
    GATGCTGACG CCAACTTTGA GCAGCTGATG GTGAACATGC TGGATGAGCG GGAGAAGTTG
    CTAGAGTCCC TTCGGGAGAG TCAGGAGACT TTGCTGGCGA CACAGAGCCG GCTCCAGGAT
    GCCCTGCATG AGCGAGACCA GCTCCAGCGC CACCTTAACT CTGCCCTCCC CCAGGAGTTT
    ACCACCTTAA CCCGGGAGCT GAGTATGTGT CGGGAGCAGC TTCTAGAGAG GGAGGAAGAG
    ATATCAGAGC TGAAAGCAGA GCGGAATAAC ACGAGGTTGC TTCTGGAACA TCTGGAGTGC
    CTGGTGTCCC GCCATGAGCG GTCACTGAGG ATGACTGTGG TGAAGCGTCA GGCCCAGTCA
    CCCTCAGGGG TCTCCAGTGA GGTGGAGGTG CTGAAGGCTC TCAAGTCACT GTTTGAGCAC
    CACAAGGCCC TGGATGAGAA GGTGCGAGAG CGGCTCCGGG TGGCCCTCGA GAGAGTGACC
    ACTTTGGAGG AACAGCTGGC GTGTGCCCAC CAGCAGGTGT CTTCCCTACA GCAGGGGTCA
    GGGGTTCGGG ATGGAGTGGC CGAAGAGGAG GGGACTGTGG AGCTGGGATC AAAAGGCCTG
    TGGAAGGAGG ACACAGGCCA GGTTGAGGAG CTGCAGGAGC TTCTGGAGAA GCAGAACTTT
    GAGTTGAGCC AGGCCCGGGA GCGACTGGTC ACCCTGACAG CAACTGTGGC TGAACTGGAG
    GAGGACCTGG GCACAGCCCG CCGGGACCTC ATCAAGTCGG AGGAACTGAG CAGCAAACAC
    CAGCGGGACC TCCGGGAAGC TCTAGCCCAG AAAGAGGACA TGGAGGAGCG GATCACCACC
    CTGGAGAAGC GCTACCTGGC TGCTCAGCGT GAGGCTACAT CAATACATGA CCTCAATGAC
    AAACTGGAGA ACGAGCTAGC CAATAGGGAA TCCTTGCATC GCCAGTGTGA GGAGAAAGCC
    CGATATCTGC AGGAGCTACT GGAGGTAGCC GAGCAGAAAC TTCAGCAGAC AATGCGCAAG
    GCCGAGACGC TGCCAGAGGT GGAGGCTGAG CTGGCCCAAA GAGTTGCGGC CCTCACCAAG
    GCTGAAGAGC GACATGGCAA CACTGAGGAG CACCTCCGAC AGCTGGAGGG ACAGCTGGAG
    GAGAAGAACC AAGAGCTGGC GCGGGTGCGC CAGCGGGAGA GGATGAATGA AGCCCACAAC
    AAGCGCCTGT CAGACACTGT GGACCGGCTG CTTATCGAGG CGAAGGAGCG CCTGCAGCTC
    CACCTCAGGG AGCGCATGGT GGCCCTGGAG GAGAAGAACA CATTGATCCA AGAGCTGGAG
    ACCTCCCAGC GGCAGATTGA AGAGCAGCAC CATCACAAGG GCCGCCTGTC TGAAGAGATT
    GAGAAACTGC GCCAAGAGGT GGACCAACTG AAGGGTCGAG GAGGACCATT TGTGGATGGC
    ATCCACTCCA GGGCGTACAC GGGCAGTGCA ATGGATGTGC GGTTCTCCCT GAGCACAACT
    GCCCACACAT CTCAAGGTCT GCGTCGTTGT TACTCAGCAC GGCGGGAACC GCCTGCCAAG
    GACTGGGAGC CATCTCCACG GCCTGCGGTG CTGGTCCCGA CAACCACCCC TGCCTTTGAC
    AGTGACCGTG AGATCTCTGA TGTGGATGAG GATGAACCAG GGGGTCTGGT GGGCTCCATG
    GACGTTGTCT CCCCCAGCAG CCACTCAGAC GCCCAGACCC TGGCCATGAT GCTGCAGGAG
    CAGCTGGATG CTATCAACGA GGAGATCAGG ATGATCCAGG AAGAGAAGGA GTCCACAGAG
    CTCCGTGCTG AGGAGCTGGA AACTCGAGTG ACTAGTGGCA GCATGGAGGC CCTAGACCTG
    ACCCAGCTGC ACAAACGCGG TTCCATCCCC ACCTCTCTGA CCGCCCTGTC CCTGGCCAGC
    GCGTCCCCTC CACTTAGCGG CCGTGCCACA CCTAAGCTCA CCTCCCGCAG TGCTGCCCAG
    GACCTGGATC GGATGGGGGT CATGACCTTG CCCAGTGACT TAAGAAAGCA TAGGAGGAAG
    CTGCTGTCAC CAGTGTCTTG GGAAGAGAAC CGAGAGGATA AAGCCACCAT AAAATGTGAG
    ACTTCCCCTC CTTCCTCACC CAGGACACTG CAGCTAGAGA AGCTTGGCCT CCCTGCCCTG
    AGCCAGGAAG AAGGCAAGAG TGCTTTGGAG GATCCAGGCA GCAACCCCAG CAGCAGCAAC
    AGCAGCCAGG ACTCCTTGCA CAAGGGTGCC AAGCGCAAGG GCATCAAATC ATCCATCGGC
    CGCCTGTTTG GGAAGAAGGA GAAAGGCAGG CTGATCCAGC CGAGCCGGGA TGGACCCACA
    GGCCATGTTG TACTACTAAC AGACTCTGAG CTCGGTCTGC AGGAGCCCAT GGTGCCTGGC
    AAGCTGGGAA CCCAGGCAGA AAAGGACCGG AGGCTGAAGA AGAAACACCA GCTGCTTGAA
    GATGCCCGGA GAAAAGGAAT GCCCTTTGCC CAGTGGGACG GCCCTACTGT GGTCTCCTGG
    CTAGAGCTCT GGGTGGGGAT GCCTGCTTGG TATGTGGCAG CCTGCCGGGC CAACGTCAAG
    AGTGGCGCCA TCATGTCAGC CCTATCGGAC ACAGAGATCC AGCGAGAAAT TGGCATCAGC
    AATGCCCTGC ACCGGCTCAA GCTCCGGCTG GCCATCCAGG AGATGGTGTC GCTGACCAGC
    CCCTCTGCCC CGCCCACCTC CAGGACTTCT TCTGGGAATG TCTGGGTCAC CCACGAAGAG
    ATGGAAACTC TGGCAACGTC CACTAAAACA GACAGTGAGG AGGGCAGCTG GGCTCAGACC
    CTGGTCTATG GGGATATGAA CCACGAGTGG ATTGGGAACC AGTGGCTCCC CAGCCTGGGG
    CTCCCCCAGT ACCGCAGCTA CTTCATGGAG TGCCTCGTGG ACGCTCGCAT GCTGGATCAC
    CTCACCAAGA AGGACTTGCG GGTCCACCTG AAGATGGTGG ACAGCTCCCA CCGAACCAGT
    CTTCAGTATG GCATCATGTG CCTGAAGAGG CTGAATTATG ACCGGAAGGA GCTGGAGAAG
    CGGCGGGAGG AGAGCCAGCA TGAGATCAAG GATGTGCTGG TCTGGACCAA TGACCAGGTA
    GTTCATTGGG TCCAGTCTAT TGGGCTCCGA GACTACGCAG GAAACCTCCA TGAGAGTGGT
    GTGCATGGCG CTTTGCTGGC CCTGGATGAG AACTTTGACC ACAACACACT GGCCCTGGTT
    CTCCAGATAC CCACACAAAA TACCCAGGCA CGCCAAGTGT TGGAGAGAGA GTTCAACAAC
    CTGTTGGCTT TGGGCACAGA CCGGAAGCTG GATGATGGGG AAGACAAGGC GCTACGCCGC
    GCGCCCTCCT GGAGGAAACG CTTCCGCCCG CGGGACCCCC ACGGCCGCGC CTCGCTCAGC
    GCCGCGGCCG ACACGCTCCC GGCAACCTTC CGCGCGTCCA CCGTGGGGCC CGTGCCGCCT
    CCAGCATCCC CGCGGAAGAA GATCACGCCT GAAGGTCTGG ACCGCTCACT CCACCGGAGA
    CCAGGGTGCA GAACCAAGGG CTGGGGTGGC CCTTCCAAAG AAAGGGCGCT TCAGACTTCC
    TTGCTTGGGT TATTGGGCCT TCTGGTTTCT GTCCGTCTCT TGGTTGCACT AGATGGTCTA
    CGTCTGCTTT TGGCATCCCC CCTCCCGCCA TTTTCTCTCC CCCAACTCAT CCGCACCTTG
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu41794
    Clone ID Related Accession (Same CDS sequence) XM_025277808.1 , XM_025277811.1 , XM_025277809.1 , XM_025277810.1
    Accession Version XM_025277810.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3783bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product liprin-alpha-4 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_020228958.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGTGTGAAG TGATGCCCAC AATCAACGAG GGAGACCCCC TGGGGCCTCC CCATGGAGCC 
    GATGCTGACG CCAACTTTGA GCAGCTGATG GTGAACATGC TGGATGAGCG GGAGAAGTTG
    CTAGAGTCCC TTCGGGAGAG TCAGGAGACT TTGCTGGCGA CACAGAGCCG GCTCCAGGAT
    GCCCTGCATG AGCGAGACCA GCTCCAGCGC CACCTTAACT CTGCCCTCCC CCAGGAGTTT
    ACCACCTTAA CCCGGGAGCT GAGTATGTGT CGGGAGCAGC TTCTAGAGAG GGAGGAAGAG
    ATATCAGAGC TGAAAGCAGA GCGGAATAAC ACGAGGTTGC TTCTGGAACA TCTGGAGTGC
    CTGGTGTCCC GCCATGAGCG GTCACTGAGG ATGACTGTGG TGAAGCGTCA GGCCCAGTCA
    CCCTCAGGGG TCTCCAGTGA GGTGGAGGTG CTGAAGGCTC TCAAGTCACT GTTTGAGCAC
    CACAAGGCCC TGGATGAGAA GGTGCGAGAG CGGCTCCGGG TGGCCCTCGA GAGAGTGACC
    ACTTTGGAGG AACAGCTGGC GTGTGCCCAC CAGCAGGTGT CTTCCCTACA GCAGGGGTCA
    GGGGTTCGGG ATGGAGTGGC CGAAGAGGAG GGGACTGTGG AGCTGGGATC AAAAGGCCTG
    TGGAAGGAGG ACACAGGCCA GGTTGAGGAG CTGCAGGAGC TTCTGGAGAA GCAGAACTTT
    GAGTTGAGCC AGGCCCGGGA GCGACTGGTC ACCCTGACAG CAACTGTGGC TGAACTGGAG
    GAGGACCTGG GCACAGCCCG CCGGGACCTC ATCAAGTCGG AGGAACTGAG CAGCAAACAC
    CAGCGGGACC TCCGGGAAGC TCTAGCCCAG AAAGAGGACA TGGAGGAGCG GATCACCACC
    CTGGAGAAGC GCTACCTGGC TGCTCAGCGT GAGGCTACAT CAATACATGA CCTCAATGAC
    AAACTGGAGA ACGAGCTAGC CAATAGGGAA TCCTTGCATC GCCAGTGTGA GGAGAAAGCC
    CGATATCTGC AGGAGCTACT GGAGGTAGCC GAGCAGAAAC TTCAGCAGAC AATGCGCAAG
    GCCGAGACGC TGCCAGAGGT GGAGGCTGAG CTGGCCCAAA GAGTTGCGGC CCTCACCAAG
    GCTGAAGAGC GACATGGCAA CACTGAGGAG CACCTCCGAC AGCTGGAGGG ACAGCTGGAG
    GAGAAGAACC AAGAGCTGGC GCGGGTGCGC CAGCGGGAGA GGATGAATGA AGCCCACAAC
    AAGCGCCTGT CAGACACTGT GGACCGGCTG CTTATCGAGG CGAAGGAGCG CCTGCAGCTC
    CACCTCAGGG AGCGCATGGT GGCCCTGGAG GAGAAGAACA CATTGATCCA AGAGCTGGAG
    ACCTCCCAGC GGCAGATTGA AGAGCAGCAC CATCACAAGG GCCGCCTGTC TGAAGAGATT
    GAGAAACTGC GCCAAGAGGT GGACCAACTG AAGGGTCGAG GAGGACCATT TGTGGATGGC
    ATCCACTCCA GGGCGTACAC GGGCAGTGCA ATGGATGTGC GGTTCTCCCT GAGCACAACT
    GCCCACACAT CTCAAGGTCT GCGTCGTTGT TACTCAGCAC GGCGGGAACC GCCTGCCAAG
    GACTGGGAGC CATCTCCACG GCCTGCGGTG CTGGTCCCGA CAACCACCCC TGCCTTTGAC
    AGTGACCGTG AGATCTCTGA TGTGGATGAG GATGAACCAG GGGGTCTGGT GGGCTCCATG
    GACGTTGTCT CCCCCAGCAG CCACTCAGAC GCCCAGACCC TGGCCATGAT GCTGCAGGAG
    CAGCTGGATG CTATCAACGA GGAGATCAGG ATGATCCAGG AAGAGAAGGA GTCCACAGAG
    CTCCGTGCTG AGGAGCTGGA AACTCGAGTG ACTAGTGGCA GCATGGAGGC CCTAGACCTG
    ACCCAGCTGC ACAAACGCGG TTCCATCCCC ACCTCTCTGA CCGCCCTGTC CCTGGCCAGC
    GCGTCCCCTC CACTTAGCGG CCGTGCCACA CCTAAGCTCA CCTCCCGCAG TGCTGCCCAG
    GACCTGGATC GGATGGGGGT CATGACCTTG CCCAGTGACT TAAGAAAGCA TAGGAGGAAG
    CTGCTGTCAC CAGTGTCTTG GGAAGAGAAC CGAGAGGATA AAGCCACCAT AAAATGTGAG
    ACTTCCCCTC CTTCCTCACC CAGGACACTG CAGCTAGAGA AGCTTGGCCT CCCTGCCCTG
    AGCCAGGAAG AAGGCAAGAG TGCTTTGGAG GATCCAGGCA GCAACCCCAG CAGCAGCAAC
    AGCAGCCAGG ACTCCTTGCA CAAGGGTGCC AAGCGCAAGG GCATCAAATC ATCCATCGGC
    CGCCTGTTTG GGAAGAAGGA GAAAGGCAGG CTGATCCAGC CGAGCCGGGA TGGACCCACA
    GGCCATGTTG TACTACTAAC AGACTCTGAG CTCGGTCTGC AGGAGCCCAT GGTGCCTGGC
    AAGCTGGGAA CCCAGGCAGA AAAGGACCGG AGGCTGAAGA AGAAACACCA GCTGCTTGAA
    GATGCCCGGA GAAAAGGAAT GCCCTTTGCC CAGTGGGACG GCCCTACTGT GGTCTCCTGG
    CTAGAGCTCT GGGTGGGGAT GCCTGCTTGG TATGTGGCAG CCTGCCGGGC CAACGTCAAG
    AGTGGCGCCA TCATGTCAGC CCTATCGGAC ACAGAGATCC AGCGAGAAAT TGGCATCAGC
    AATGCCCTGC ACCGGCTCAA GCTCCGGCTG GCCATCCAGG AGATGGTGTC GCTGACCAGC
    CCCTCTGCCC CGCCCACCTC CAGGACTTCT TCTGGGAATG TCTGGGTCAC CCACGAAGAG
    ATGGAAACTC TGGCAACGTC CACTAAAACA GACAGTGAGG AGGGCAGCTG GGCTCAGACC
    CTGGTCTATG GGGATATGAA CCACGAGTGG ATTGGGAACC AGTGGCTCCC CAGCCTGGGG
    CTCCCCCAGT ACCGCAGCTA CTTCATGGAG TGCCTCGTGG ACGCTCGCAT GCTGGATCAC
    CTCACCAAGA AGGACTTGCG GGTCCACCTG AAGATGGTGG ACAGCTCCCA CCGAACCAGT
    CTTCAGTATG GCATCATGTG CCTGAAGAGG CTGAATTATG ACCGGAAGGA GCTGGAGAAG
    CGGCGGGAGG AGAGCCAGCA TGAGATCAAG GATGTGCTGG TCTGGACCAA TGACCAGGTA
    GTTCATTGGG TCCAGTCTAT TGGGCTCCGA GACTACGCAG GAAACCTCCA TGAGAGTGGT
    GTGCATGGCG CTTTGCTGGC CCTGGATGAG AACTTTGACC ACAACACACT GGCCCTGGTT
    CTCCAGATAC CCACACAAAA TACCCAGGCA CGCCAAGTGT TGGAGAGAGA GTTCAACAAC
    CTGTTGGCTT TGGGCACAGA CCGGAAGCTG GATGATGGGG AAGACAAGGC GCTACGCCGC
    GCGCCCTCCT GGAGGAAACG CTTCCGCCCG CGGGACCCCC ACGGCCGCGC CTCGCTCAGC
    GCCGCGGCCG ACACGCTCCC GGCAACCTTC CGCGCGTCCA CCGTGGGGCC CGTGCCGCCT
    CCAGCATCCC CGCGGAAGAA GATCACGCCT GAAGGTCTGG ACCGCTCACT CCACCGGAGA
    CCAGGGTGCA GAACCAAGGG CTGGGGTGGC CCTTCCAAAG AAAGGGCGCT TCAGACTTCC
    TTGCTTGGGT TATTGGGCCT TCTGGTTTCT GTCCGTCTCT TGGTTGCACT AGATGGTCTA
    CGTCTGCTTT TGGCATCCCC CCTCCCGCCA TTTTCTCTCC CCCAACTCAT CCGCACCTTG
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025133595.1
    CDS292..4074
    Translation

    Target ORF information:

    RefSeq Version XM_025277810.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis PTPRF interacting protein alpha 4 (PPFIA4), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025277810.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGTGTGAAG TGATGCCCAC AATCAACGAG GGAGACCCCC TGGGGCCTCC CCATGGAGCC 
    GATGCTGACG CCAACTTTGA GCAGCTGATG GTGAACATGC TGGATGAGCG GGAGAAGTTG
    CTAGAGTCCC TTCGGGAGAG TCAGGAGACT TTGCTGGCGA CACAGAGCCG GCTCCAGGAT
    GCCCTGCATG AGCGAGACCA GCTCCAGCGC CACCTTAACT CTGCCCTCCC CCAGGAGTTT
    ACCACCTTAA CCCGGGAGCT GAGTATGTGT CGGGAGCAGC TTCTAGAGAG GGAGGAAGAG
    ATATCAGAGC TGAAAGCAGA GCGGAATAAC ACGAGGTTGC TTCTGGAACA TCTGGAGTGC
    CTGGTGTCCC GCCATGAGCG GTCACTGAGG ATGACTGTGG TGAAGCGTCA GGCCCAGTCA
    CCCTCAGGGG TCTCCAGTGA GGTGGAGGTG CTGAAGGCTC TCAAGTCACT GTTTGAGCAC
    CACAAGGCCC TGGATGAGAA GGTGCGAGAG CGGCTCCGGG TGGCCCTCGA GAGAGTGACC
    ACTTTGGAGG AACAGCTGGC GTGTGCCCAC CAGCAGGTGT CTTCCCTACA GCAGGGGTCA
    GGGGTTCGGG ATGGAGTGGC CGAAGAGGAG GGGACTGTGG AGCTGGGATC AAAAGGCCTG
    TGGAAGGAGG ACACAGGCCA GGTTGAGGAG CTGCAGGAGC TTCTGGAGAA GCAGAACTTT
    GAGTTGAGCC AGGCCCGGGA GCGACTGGTC ACCCTGACAG CAACTGTGGC TGAACTGGAG
    GAGGACCTGG GCACAGCCCG CCGGGACCTC ATCAAGTCGG AGGAACTGAG CAGCAAACAC
    CAGCGGGACC TCCGGGAAGC TCTAGCCCAG AAAGAGGACA TGGAGGAGCG GATCACCACC
    CTGGAGAAGC GCTACCTGGC TGCTCAGCGT GAGGCTACAT CAATACATGA CCTCAATGAC
    AAACTGGAGA ACGAGCTAGC CAATAGGGAA TCCTTGCATC GCCAGTGTGA GGAGAAAGCC
    CGATATCTGC AGGAGCTACT GGAGGTAGCC GAGCAGAAAC TTCAGCAGAC AATGCGCAAG
    GCCGAGACGC TGCCAGAGGT GGAGGCTGAG CTGGCCCAAA GAGTTGCGGC CCTCACCAAG
    GCTGAAGAGC GACATGGCAA CACTGAGGAG CACCTCCGAC AGCTGGAGGG ACAGCTGGAG
    GAGAAGAACC AAGAGCTGGC GCGGGTGCGC CAGCGGGAGA GGATGAATGA AGCCCACAAC
    AAGCGCCTGT CAGACACTGT GGACCGGCTG CTTATCGAGG CGAAGGAGCG CCTGCAGCTC
    CACCTCAGGG AGCGCATGGT GGCCCTGGAG GAGAAGAACA CATTGATCCA AGAGCTGGAG
    ACCTCCCAGC GGCAGATTGA AGAGCAGCAC CATCACAAGG GCCGCCTGTC TGAAGAGATT
    GAGAAACTGC GCCAAGAGGT GGACCAACTG AAGGGTCGAG GAGGACCATT TGTGGATGGC
    ATCCACTCCA GGGCGTACAC GGGCAGTGCA ATGGATGTGC GGTTCTCCCT GAGCACAACT
    GCCCACACAT CTCAAGGTCT GCGTCGTTGT TACTCAGCAC GGCGGGAACC GCCTGCCAAG
    GACTGGGAGC CATCTCCACG GCCTGCGGTG CTGGTCCCGA CAACCACCCC TGCCTTTGAC
    AGTGACCGTG AGATCTCTGA TGTGGATGAG GATGAACCAG GGGGTCTGGT GGGCTCCATG
    GACGTTGTCT CCCCCAGCAG CCACTCAGAC GCCCAGACCC TGGCCATGAT GCTGCAGGAG
    CAGCTGGATG CTATCAACGA GGAGATCAGG ATGATCCAGG AAGAGAAGGA GTCCACAGAG
    CTCCGTGCTG AGGAGCTGGA AACTCGAGTG ACTAGTGGCA GCATGGAGGC CCTAGACCTG
    ACCCAGCTGC ACAAACGCGG TTCCATCCCC ACCTCTCTGA CCGCCCTGTC CCTGGCCAGC
    GCGTCCCCTC CACTTAGCGG CCGTGCCACA CCTAAGCTCA CCTCCCGCAG TGCTGCCCAG
    GACCTGGATC GGATGGGGGT CATGACCTTG CCCAGTGACT TAAGAAAGCA TAGGAGGAAG
    CTGCTGTCAC CAGTGTCTTG GGAAGAGAAC CGAGAGGATA AAGCCACCAT AAAATGTGAG
    ACTTCCCCTC CTTCCTCACC CAGGACACTG CAGCTAGAGA AGCTTGGCCT CCCTGCCCTG
    AGCCAGGAAG AAGGCAAGAG TGCTTTGGAG GATCCAGGCA GCAACCCCAG CAGCAGCAAC
    AGCAGCCAGG ACTCCTTGCA CAAGGGTGCC AAGCGCAAGG GCATCAAATC ATCCATCGGC
    CGCCTGTTTG GGAAGAAGGA GAAAGGCAGG CTGATCCAGC CGAGCCGGGA TGGACCCACA
    GGCCATGTTG TACTACTAAC AGACTCTGAG CTCGGTCTGC AGGAGCCCAT GGTGCCTGGC
    AAGCTGGGAA CCCAGGCAGA AAAGGACCGG AGGCTGAAGA AGAAACACCA GCTGCTTGAA
    GATGCCCGGA GAAAAGGAAT GCCCTTTGCC CAGTGGGACG GCCCTACTGT GGTCTCCTGG
    CTAGAGCTCT GGGTGGGGAT GCCTGCTTGG TATGTGGCAG CCTGCCGGGC CAACGTCAAG
    AGTGGCGCCA TCATGTCAGC CCTATCGGAC ACAGAGATCC AGCGAGAAAT TGGCATCAGC
    AATGCCCTGC ACCGGCTCAA GCTCCGGCTG GCCATCCAGG AGATGGTGTC GCTGACCAGC
    CCCTCTGCCC CGCCCACCTC CAGGACTTCT TCTGGGAATG TCTGGGTCAC CCACGAAGAG
    ATGGAAACTC TGGCAACGTC CACTAAAACA GACAGTGAGG AGGGCAGCTG GGCTCAGACC
    CTGGTCTATG GGGATATGAA CCACGAGTGG ATTGGGAACC AGTGGCTCCC CAGCCTGGGG
    CTCCCCCAGT ACCGCAGCTA CTTCATGGAG TGCCTCGTGG ACGCTCGCAT GCTGGATCAC
    CTCACCAAGA AGGACTTGCG GGTCCACCTG AAGATGGTGG ACAGCTCCCA CCGAACCAGT
    CTTCAGTATG GCATCATGTG CCTGAAGAGG CTGAATTATG ACCGGAAGGA GCTGGAGAAG
    CGGCGGGAGG AGAGCCAGCA TGAGATCAAG GATGTGCTGG TCTGGACCAA TGACCAGGTA
    GTTCATTGGG TCCAGTCTAT TGGGCTCCGA GACTACGCAG GAAACCTCCA TGAGAGTGGT
    GTGCATGGCG CTTTGCTGGC CCTGGATGAG AACTTTGACC ACAACACACT GGCCCTGGTT
    CTCCAGATAC CCACACAAAA TACCCAGGCA CGCCAAGTGT TGGAGAGAGA GTTCAACAAC
    CTGTTGGCTT TGGGCACAGA CCGGAAGCTG GATGATGGGG AAGACAAGGC GCTACGCCGC
    GCGCCCTCCT GGAGGAAACG CTTCCGCCCG CGGGACCCCC ACGGCCGCGC CTCGCTCAGC
    GCCGCGGCCG ACACGCTCCC GGCAACCTTC CGCGCGTCCA CCGTGGGGCC CGTGCCGCCT
    CCAGCATCCC CGCGGAAGAA GATCACGCCT GAAGGTCTGG ACCGCTCACT CCACCGGAGA
    CCAGGGTGCA GAACCAAGGG CTGGGGTGGC CCTTCCAAAG AAAGGGCGCT TCAGACTTCC
    TTGCTTGGGT TATTGGGCCT TCTGGTTTCT GTCCGTCTCT TGGTTGCACT AGATGGTCTA
    CGTCTGCTTT TGGCATCCCC CCTCCCGCCA TTTTCTCTCC CCCAACTCAT CCGCACCTTG
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu41795
    Clone ID Related Accession (Same CDS sequence) XM_025277812.1
    Accession Version XM_025277812.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3735bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product liprin-alpha-4 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_020228958.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGAGGCAG GGCTCCCTTG GGCCCTAAAA CTTGACTGGC GTTGGGTAAA AATCTGGCTC 
    CCCGCCACCT CACGAGATAC TCTCCCGGCC GAGCCAAGAT GGCGCGCAGC CCCAAGTGGC
    TCCACGTCAC GAGGGAGGGA GCGCCGGGGC GGGGGCGTGG CCTCGAGGGG GCCGGCTAGC
    CCCGCCCTTG AGGTGGGGCC GGGGGCCGTC CGCAGAAGGC GAGGGCGGGG CGCTTCGGCT
    CGGGAAGGCT GCGGCTGCCT CGGCGCCGGC TCCGCTCGCG AGGACAAGTC CGGCGCCTCC
    AGGCCGGGCC GGCTTCCTGC GCAGAGCCAA GCTGCCCGGC CTTCGGAGTT TACCACCTTA
    ACCCGGGAGC TGAGTATGTG TCGGGAGCAG CTTCTAGAGA GGGAGGAAGA GATATCAGAG
    CTGAAAGCAG AGCGGAATAA CACGAGGTTG CTTCTGGAAC ATCTGGAGTG CCTGGTGTCC
    CGCCATGAGC GGTCACTGAG GATGACTGTG GTGAAGCGTC AGGCCCAGTC ACCCTCAGGG
    GTCTCCAGTG AGGTGGAGGT GCTGAAGGCT CTCAAGTCAC TGTTTGAGCA CCACAAGGCC
    CTGGATGAGA AGGTGCGAGA GCGGCTCCGG GTGGCCCTCG AGAGAGTGAC CACTTTGGAG
    GAACAGCTGG CGTGTGCCCA CCAGCAGGTG TCTTCCCTAC AGCAGGGGTC AGGGGTTCGG
    GATGGAGTGG CCGAAGAGGA GGGGACTGTG GAGCTGGGAT CAAAAGGCCT GTGGAAGGAG
    GACACAGGCC AGGTTGAGGA GCTGCAGGAG CTTCTGGAGA AGCAGAACTT TGAGTTGAGC
    CAGGCCCGGG AGCGACTGGT CACCCTGACA GCAACTGTGG CTGAACTGGA GGAGGACCTG
    GGCACAGCCC GCCGGGACCT CATCAAGTCG GAGGAACTGA GCAGCAAACA CCAGCGGGAC
    CTCCGGGAAG CTCTAGCCCA GAAAGAGGAC ATGGAGGAGC GGATCACCAC CCTGGAGAAG
    CGCTACCTGG CTGCTCAGCG TGAGGCTACA TCAATACATG ACCTCAATGA CAAACTGGAG
    AACGAGCTAG CCAATAGGGA ATCCTTGCAT CGCCAGTGTG AGGAGAAAGC CCGATATCTG
    CAGGAGCTAC TGGAGGTAGC CGAGCAGAAA CTTCAGCAGA CAATGCGCAA GGCCGAGACG
    CTGCCAGAGG TGGAGGCTGA GCTGGCCCAA AGAGTTGCGG CCCTCACCAA GGCTGAAGAG
    CGACATGGCA ACACTGAGGA GCACCTCCGA CAGCTGGAGG GACAGCTGGA GGAGAAGAAC
    CAAGAGCTGG CGCGGGTGCG CCAGCGGGAG AGGATGAATG AAGCCCACAA CAAGCGCCTG
    TCAGACACTG TGGACCGGCT GCTTATCGAG GCGAAGGAGC GCCTGCAGCT CCACCTCAGG
    GAGCGCATGG TGGCCCTGGA GGAGAAGAAC ACATTGATCC AAGAGCTGGA GACCTCCCAG
    CGGCAGATTG AAGAGCAGCA CCATCACAAG GGCCGCCTGT CTGAAGAGAT TGAGAAACTG
    CGCCAAGAGG TGGACCAACT GAAGGGTCGA GGAGGACCAT TTGTGGATGG CATCCACTCC
    AGGGCGTACA CGGGCAGTGC AATGGATGTG CGGTTCTCCC TGAGCACAAC TGCCCACACA
    TCTCAAGGTC TGCGTCGTTG TTACTCAGCA CGGCGGGAAC CGCCTGCCAA GGACTGGGAG
    CCATCTCCAC GGCCTGCGGT GCTGGTCCCG ACAACCACCC CTGCCTTTGA CAGTGACCGT
    GAGATCTCTG ATGTGGATGA GGATGAACCA GGGGGTCTGG TGGGCTCCAT GGACGTTGTC
    TCCCCCAGCA GCCACTCAGA CGCCCAGACC CTGGCCATGA TGCTGCAGGA GCAGCTGGAT
    GCTATCAACG AGGAGATCAG GATGATCCAG GAAGAGAAGG AGTCCACAGA GCTCCGTGCT
    GAGGAGCTGG AAACTCGAGT GACTAGTGGC AGCATGGAGG CCCTAGACCT GACCCAGCTG
    CACAAACGCG GTTCCATCCC CACCTCTCTG ACCGCCCTGT CCCTGGCCAG CGCGTCCCCT
    CCACTTAGCG GCCGTGCCAC ACCTAAGCTC ACCTCCCGCA GTGCTGCCCA GGACCTGGAT
    CGGATGGGGG TCATGACCTT GCCCAGTGAC TTAAGAAAGC ATAGGAGGAA GCTGCTGTCA
    CCAGTGTCTT GGGAAGAGAA CCGAGAGGAT AAAGCCACCA TAAAATGTGA GACTTCCCCT
    CCTTCCTCAC CCAGGACACT GCAGCTAGAG AAGCTTGGCC TCCCTGCCCT GAGCCAGGAA
    GAAGGCAAGA GTGCTTTGGA GGATCCAGGC AGCAACCCCA GCAGCAGCAA CAGCAGCCAG
    GACTCCTTGC ACAAGGGTGC CAAGCGCAAG GGCATCAAAT CATCCATCGG CCGCCTGTTT
    GGGAAGAAGG AGAAAGGCAG GCTGATCCAG CCGAGCCGGG ATGGACCCAC AGGCCATGTT
    GTACTACTAA CAGACTCTGA GCTCGGTCTG CAGGAGCCCA TGGTGCCTGG CAAGCTGGGA
    ACCCAGGCAG AAAAGGACCG GAGGCTGAAG AAGAAACACC AGCTGCTTGA AGATGCCCGG
    AGAAAAGGAA TGCCCTTTGC CCAGTGGGAC GGCCCTACTG TGGTCTCCTG GCTAGAGCTC
    TGGGTGGGGA TGCCTGCTTG GTATGTGGCA GCCTGCCGGG CCAACGTCAA GAGTGGCGCC
    ATCATGTCAG CCCTATCGGA CACAGAGATC CAGCGAGAAA TTGGCATCAG CAATGCCCTG
    CACCGGCTCA AGCTCCGGCT GGCCATCCAG GAGATGGTGT CGCTGACCAG CCCCTCTGCC
    CCGCCCACCT CCAGGACTTC TTCTGGGAAT GTCTGGGTCA CCCACGAAGA GATGGAAACT
    CTGGCAACGT CCACTAAAAC AGACAGTGAG GAGGGCAGCT GGGCTCAGAC CCTGGTCTAT
    GGGGATATGA ACCACGAGTG GATTGGGAAC CAGTGGCTCC CCAGCCTGGG GCTCCCCCAG
    TACCGCAGCT ACTTCATGGA GTGCCTCGTG GACGCTCGCA TGCTGGATCA CCTCACCAAG
    AAGGACTTGC GGGTCCACCT GAAGATGGTG GACAGCTCCC ACCGAACCAG TCTTCAGTAT
    GGCATCATGT GCCTGAAGAG GCTGAATTAT GACCGGAAGG AGCTGGAGAA GCGGCGGGAG
    GAGAGCCAGC ATGAGATCAA GGATGTGCTG GTCTGGACCA ATGACCAGGT AGTTCATTGG
    GTCCAGTCTA TTGGGCTCCG AGACTACGCA GGAAACCTCC ATGAGAGTGG TGTGCATGGC
    GCTTTGCTGG CCCTGGATGA GAACTTTGAC CACAACACAC TGGCCCTGGT TCTCCAGATA
    CCCACACAAA ATACCCAGGC ACGCCAAGTG TTGGAGAGAG AGTTCAACAA CCTGTTGGCT
    TTGGGCACAG ACCGGAAGCT GGATGATGGG GAAGACAAGG CGCTACGCCG CGCGCCCTCC
    TGGAGGAAAC GCTTCCGCCC GCGGGACCCC CACGGCCGCG CCTCGCTCAG CGCCGCGGCC
    GACACGCTCC CGGCAACCTT CCGCGCGTCC ACCGTGGGGC CCGTGCCGCC TCCAGCATCC
    CCGCGGAAGA AGATCACGCC TGAAGCTCGC TCCCACTATC TCTACGGACA CATGCTCTCC
    GCCTTCCGGG ACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025133597.1
    CDS564..4298
    Translation

    Target ORF information:

    RefSeq Version XM_025277812.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis PTPRF interacting protein alpha 4 (PPFIA4), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025277812.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGAGGCAG GGCTCCCTTG GGCCCTAAAA CTTGACTGGC GTTGGGTAAA AATCTGGCTC 
    CCCGCCACCT CACGAGATAC TCTCCCGGCC GAGCCAAGAT GGCGCGCAGC CCCAAGTGGC
    TCCACGTCAC GAGGGAGGGA GCGCCGGGGC GGGGGCGTGG CCTCGAGGGG GCCGGCTAGC
    CCCGCCCTTG AGGTGGGGCC GGGGGCCGTC CGCAGAAGGC GAGGGCGGGG CGCTTCGGCT
    CGGGAAGGCT GCGGCTGCCT CGGCGCCGGC TCCGCTCGCG AGGACAAGTC CGGCGCCTCC
    AGGCCGGGCC GGCTTCCTGC GCAGAGCCAA GCTGCCCGGC CTTCGGAGTT TACCACCTTA
    ACCCGGGAGC TGAGTATGTG TCGGGAGCAG CTTCTAGAGA GGGAGGAAGA GATATCAGAG
    CTGAAAGCAG AGCGGAATAA CACGAGGTTG CTTCTGGAAC ATCTGGAGTG CCTGGTGTCC
    CGCCATGAGC GGTCACTGAG GATGACTGTG GTGAAGCGTC AGGCCCAGTC ACCCTCAGGG
    GTCTCCAGTG AGGTGGAGGT GCTGAAGGCT CTCAAGTCAC TGTTTGAGCA CCACAAGGCC
    CTGGATGAGA AGGTGCGAGA GCGGCTCCGG GTGGCCCTCG AGAGAGTGAC CACTTTGGAG
    GAACAGCTGG CGTGTGCCCA CCAGCAGGTG TCTTCCCTAC AGCAGGGGTC AGGGGTTCGG
    GATGGAGTGG CCGAAGAGGA GGGGACTGTG GAGCTGGGAT CAAAAGGCCT GTGGAAGGAG
    GACACAGGCC AGGTTGAGGA GCTGCAGGAG CTTCTGGAGA AGCAGAACTT TGAGTTGAGC
    CAGGCCCGGG AGCGACTGGT CACCCTGACA GCAACTGTGG CTGAACTGGA GGAGGACCTG
    GGCACAGCCC GCCGGGACCT CATCAAGTCG GAGGAACTGA GCAGCAAACA CCAGCGGGAC
    CTCCGGGAAG CTCTAGCCCA GAAAGAGGAC ATGGAGGAGC GGATCACCAC CCTGGAGAAG
    CGCTACCTGG CTGCTCAGCG TGAGGCTACA TCAATACATG ACCTCAATGA CAAACTGGAG
    AACGAGCTAG CCAATAGGGA ATCCTTGCAT CGCCAGTGTG AGGAGAAAGC CCGATATCTG
    CAGGAGCTAC TGGAGGTAGC CGAGCAGAAA CTTCAGCAGA CAATGCGCAA GGCCGAGACG
    CTGCCAGAGG TGGAGGCTGA GCTGGCCCAA AGAGTTGCGG CCCTCACCAA GGCTGAAGAG
    CGACATGGCA ACACTGAGGA GCACCTCCGA CAGCTGGAGG GACAGCTGGA GGAGAAGAAC
    CAAGAGCTGG CGCGGGTGCG CCAGCGGGAG AGGATGAATG AAGCCCACAA CAAGCGCCTG
    TCAGACACTG TGGACCGGCT GCTTATCGAG GCGAAGGAGC GCCTGCAGCT CCACCTCAGG
    GAGCGCATGG TGGCCCTGGA GGAGAAGAAC ACATTGATCC AAGAGCTGGA GACCTCCCAG
    CGGCAGATTG AAGAGCAGCA CCATCACAAG GGCCGCCTGT CTGAAGAGAT TGAGAAACTG
    CGCCAAGAGG TGGACCAACT GAAGGGTCGA GGAGGACCAT TTGTGGATGG CATCCACTCC
    AGGGCGTACA CGGGCAGTGC AATGGATGTG CGGTTCTCCC TGAGCACAAC TGCCCACACA
    TCTCAAGGTC TGCGTCGTTG TTACTCAGCA CGGCGGGAAC CGCCTGCCAA GGACTGGGAG
    CCATCTCCAC GGCCTGCGGT GCTGGTCCCG ACAACCACCC CTGCCTTTGA CAGTGACCGT
    GAGATCTCTG ATGTGGATGA GGATGAACCA GGGGGTCTGG TGGGCTCCAT GGACGTTGTC
    TCCCCCAGCA GCCACTCAGA CGCCCAGACC CTGGCCATGA TGCTGCAGGA GCAGCTGGAT
    GCTATCAACG AGGAGATCAG GATGATCCAG GAAGAGAAGG AGTCCACAGA GCTCCGTGCT
    GAGGAGCTGG AAACTCGAGT GACTAGTGGC AGCATGGAGG CCCTAGACCT GACCCAGCTG
    CACAAACGCG GTTCCATCCC CACCTCTCTG ACCGCCCTGT CCCTGGCCAG CGCGTCCCCT
    CCACTTAGCG GCCGTGCCAC ACCTAAGCTC ACCTCCCGCA GTGCTGCCCA GGACCTGGAT
    CGGATGGGGG TCATGACCTT GCCCAGTGAC TTAAGAAAGC ATAGGAGGAA GCTGCTGTCA
    CCAGTGTCTT GGGAAGAGAA CCGAGAGGAT AAAGCCACCA TAAAATGTGA GACTTCCCCT
    CCTTCCTCAC CCAGGACACT GCAGCTAGAG AAGCTTGGCC TCCCTGCCCT GAGCCAGGAA
    GAAGGCAAGA GTGCTTTGGA GGATCCAGGC AGCAACCCCA GCAGCAGCAA CAGCAGCCAG
    GACTCCTTGC ACAAGGGTGC CAAGCGCAAG GGCATCAAAT CATCCATCGG CCGCCTGTTT
    GGGAAGAAGG AGAAAGGCAG GCTGATCCAG CCGAGCCGGG ATGGACCCAC AGGCCATGTT
    GTACTACTAA CAGACTCTGA GCTCGGTCTG CAGGAGCCCA TGGTGCCTGG CAAGCTGGGA
    ACCCAGGCAG AAAAGGACCG GAGGCTGAAG AAGAAACACC AGCTGCTTGA AGATGCCCGG
    AGAAAAGGAA TGCCCTTTGC CCAGTGGGAC GGCCCTACTG TGGTCTCCTG GCTAGAGCTC
    TGGGTGGGGA TGCCTGCTTG GTATGTGGCA GCCTGCCGGG CCAACGTCAA GAGTGGCGCC
    ATCATGTCAG CCCTATCGGA CACAGAGATC CAGCGAGAAA TTGGCATCAG CAATGCCCTG
    CACCGGCTCA AGCTCCGGCT GGCCATCCAG GAGATGGTGT CGCTGACCAG CCCCTCTGCC
    CCGCCCACCT CCAGGACTTC TTCTGGGAAT GTCTGGGTCA CCCACGAAGA GATGGAAACT
    CTGGCAACGT CCACTAAAAC AGACAGTGAG GAGGGCAGCT GGGCTCAGAC CCTGGTCTAT
    GGGGATATGA ACCACGAGTG GATTGGGAAC CAGTGGCTCC CCAGCCTGGG GCTCCCCCAG
    TACCGCAGCT ACTTCATGGA GTGCCTCGTG GACGCTCGCA TGCTGGATCA CCTCACCAAG
    AAGGACTTGC GGGTCCACCT GAAGATGGTG GACAGCTCCC ACCGAACCAG TCTTCAGTAT
    GGCATCATGT GCCTGAAGAG GCTGAATTAT GACCGGAAGG AGCTGGAGAA GCGGCGGGAG
    GAGAGCCAGC ATGAGATCAA GGATGTGCTG GTCTGGACCA ATGACCAGGT AGTTCATTGG
    GTCCAGTCTA TTGGGCTCCG AGACTACGCA GGAAACCTCC ATGAGAGTGG TGTGCATGGC
    GCTTTGCTGG CCCTGGATGA GAACTTTGAC CACAACACAC TGGCCCTGGT TCTCCAGATA
    CCCACACAAA ATACCCAGGC ACGCCAAGTG TTGGAGAGAG AGTTCAACAA CCTGTTGGCT
    TTGGGCACAG ACCGGAAGCT GGATGATGGG GAAGACAAGG CGCTACGCCG CGCGCCCTCC
    TGGAGGAAAC GCTTCCGCCC GCGGGACCCC CACGGCCGCG CCTCGCTCAG CGCCGCGGCC
    GACACGCTCC CGGCAACCTT CCGCGCGTCC ACCGTGGGGC CCGTGCCGCC TCCAGCATCC
    CCGCGGAAGA AGATCACGCC TGAAGCTCGC TCCCACTATC TCTACGGACA CATGCTCTCC
    GCCTTCCGGG ACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu41796
    Clone ID Related Accession (Same CDS sequence) XM_025277813.1
    Accession Version XM_025277813.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3624bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product liprin-alpha-4 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_020228958.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGTGTGAAG TGATGCCCAC AATCAACGAG GGAGACCCCC TGGGGCCTCC CCATGGAGCC 
    GATGCTGACG CCAACTTTGA GCAGCTGATG GTGAACATGC TGGATGAGCG GGAGAAGTTG
    CTAGAGTCCC TTCGGGAGAG TCAGGAGACT TTGCTGGCGA CACAGAGCCG GCTCCAGGAT
    GCCCTGCATG AGCGAGACCA GCTCCAGCGC CACCTTAACT CTGCCCTCCC CCAGGAGTTT
    ACCACCTTAA CCCGGGAGCT GAGTATGTGT CGGGAGCAGC TTCTAGAGAG GGAGGAAGAG
    ATATCAGAGC TGAAAGCAGA GCGGAATAAC ACGAGGTTGC TTCTGGAACA TCTGGAGTGC
    CTGGTGTCCC GCCATGAGCG GTCACTGAGG ATGACTGTGG TGAAGCGTCA GGCCCAGTCA
    CCCTCAGGGG TCTCCAGTGA GGTGGAGGTG CTGAAGGCTC TCAAGTCACT GTTTGAGCAC
    CACAAGGCCC TGGATGAGAA GGTGCGAGAG CGGCTCCGGG TGGCCCTCGA GAGAGTGACC
    ACTTTGGAGG AACAGCTGGC GTGTGCCCAC CAGCAGGTGT CTTCCCTACA GCAGGGGTCA
    GGGGTTCGGG ATGGAGTGGC CGAAGAGGAG GGGACTGTGG AGCTGGGATC AAAAGGCCTG
    TGGAAGGAGG ACACAGGCCA GGTTGAGGAG CTGCAGGAGC TTCTGGAGAA GCAGAACTTT
    GAGTTGAGCC AGGCCCGGGA GCGACTGGTC ACCCTGACAG CAACTGTGGC TGAACTGGAG
    GAGGACCTGG GCACAGCCCG CCGGGACCTC ATCAAGTCGG AGGAACTGAG CAGCAAACAC
    CAGCGGGACC TCCGGGAAGC TCTAGCCCAG AAAGAGGACA TGGAGGAGCG GATCACCACC
    CTGGAGAAGC GCTACCTGGC TGCTCAGCGT GAGGCTACAT CAATACATGA CCTCAATGAC
    AAACTGGAGA ACGAGCTAGC CAATAGGGAA TCCTTGCATC GCCAGTGTGA GGAGAAAGCC
    CGATATCTGC AGGAGCTACT GGAGGTAGCC GAGCAGAAAC TTCAGCAGAC AATGCGCAAG
    GCCGAGACGC TGCCAGAGGT GGAGGCTGAG CTGGCCCAAA GAGTTGCGGC CCTCACCAAG
    GCTGAAGAGC GACATGGCAA CACTGAGGAG CACCTCCGAC AGCTGGAGGG ACAGCTGGAG
    GAGAAGAACC AAGAGCTGGC GCGGGTGCGC CAGCGGGAGA GGATGAATGA AGCCCACAAC
    AAGCGCCTGT CAGACACTGT GGACCGGCTG CTTATCGAGG CGAAGGAGCG CCTGCAGCTC
    CACCTCAGGG AGCGCATGGT GGCCCTGGAG GAGAAGAACA CATTGATCCA AGAGCTGGAG
    ACCTCCCAGC GGCAGATTGA AGAGCAGCAC CATCACAAGG GCCGCCTGTC TGAAGAGATT
    GAGAAACTGC GCCAAGAGGT GGACCAACTG AAGGGTCGAG GAGGACCATT TGTGGATGGC
    ATCCACTCCA GGGCGTACAC GGGCAGTGCA ATGGATGTGC GGTTCTCCCT GAGCACAACT
    GCCCACACAT CTCAAGGTCT GCGTCGTTGT TACTCAGCAC GGCGGGAACC GCCTGCCAAG
    GACTGGGAGC CATCTCCACG GCCTGCGGTG CTGGTCCCGA CAACCACCCC TGCCTTTGAC
    AGTGACCGTG AGATCTCTGA TGTGGATGAG GATGAACCAG GGGGTCTGGT GGGCTCCATG
    GACGTTGTCT CCCCCAGCAG CCACTCAGAC GCCCAGACCC TGGCCATGAT GCTGCAGGAG
    CAGCTGGATG CTATCAACGA GGAGATCAGG ATGATCCAGG AAGAGAAGGA GTCCACAGAG
    CTCCGTGCTG AGGAGCTGGA AACTCGAGTG ACTAGTGGCA GCATGGAGGC CCTAGACCTG
    ACCCAGCTGC ACAAACGCGG TTCCATCCCC ACCTCTCTGA CCGCCCTGTC CCTGGCCAGC
    GCGTCCCCTC CACTTAGCGG CCGTGCCACA CCTAAGCTCA CCTCCCGCAG TGCTGCCCAG
    GACCTGGATC GGATGGGGGT CATGACCTTG CCCAGTGACT TAAGAAAGCA TAGGAGGAAG
    CTGCTGTCAC CAGTGTCTTG GGAAGAGAAC CGAGAGGATA AAGCCACCAT AAAATGTGAG
    ACTTCCCCTC CTTCCTCACC CAGGACACTG CAGCTAGAGA AGCTTGGCCT CCCTGCCCTG
    AGCCAGGAAG AAGGCAAGAG TGCTTTGGAG GATCCAGGCA GCAACCCCAG CAGCAGCAAC
    AGCAGCCAGG ACTCCTTGCA CAAGGGTGCC AAGCGCAAGG GCATCAAATC ATCCATCGGC
    CGCCTGTTTG GGAAGAAGGA GAAAGGCAGG CTGATCCAGC CGAGCCGGGA TGGACCCACA
    GGCCATGTTG TACTACTAAC AGACTCTGAG CTCGGTCTGC AGGAGCCCAT GGTGCCTGGC
    AAGCTGGGAA CCCAGGCAGA AAAGGACCGG AGGCTGAAGA AGAAACACCA GCTGCTTGAA
    GATGCCCGGA GAAAAGGAAT GCCCTTTGCC CAGTGGGACG GCCCTACTGT GGTCTCCTGG
    CTAGAGCTCT GGGTGGGGAT GCCTGCTTGG TATGTGGCAG CCTGCCGGGC CAACGTCAAG
    AGTGGCGCCA TCATGTCAGC CCTATCGGAC ACAGAGATCC AGCGAGAAAT TGGCATCAGC
    AATGCCCTGC ACCGGCTCAA GCTCCGGCTG GCCATCCAGG AGATGGTGTC GCTGACCAGC
    CCCTCTGCCC CGCCCACCTC CAGGACTTCT TCTGGGAATG TCTGGGTCAC CCACGAAGAG
    ATGGAAACTC TGGCAACGTC CACTAAAACA GACAGTGAGG AGGGCAGCTG GGCTCAGACC
    CTGGTCTATG GGGATATGAA CCACGAGTGG ATTGGGAACC AGTGGCTCCC CAGCCTGGGG
    CTCCCCCAGT ACCGCAGCTA CTTCATGGAG TGCCTCGTGG ACGCTCGCAT GCTGGATCAC
    CTCACCAAGA AGGACTTGCG GGTCCACCTG AAGATGGTGG ACAGCTCCCA CCGAACCAGT
    CTTCAGTATG GCATCATGTG CCTGAAGAGG CTGAATTATG ACCGGAAGGA GCTGGAGAAG
    CGGCGGGAGG AGAGCCAGCA TGAGATCAAG GATGTGCTGG TCTGGACCAA TGACCAGGTA
    GTTCATTGGG TCCAGTCTAT TGGGCTCCGA GACTACGCAG GAAACCTCCA TGAGAGTGGT
    GTGCATGGCG CTTTGCTGGC CCTGGATGAG AACTTTGACC ACAACACACT GGCCCTGGTT
    CTCCAGATAC CCACACAAAA TACCCAGGCA CGCCAAGTGT TGGAGAGAGA GTTCAACAAC
    CTGTTGGCTT TGGGCACAGA CCGGAAGCTG GATGATGGGG AAGACAAGGC GCTACGCCGC
    GCGCCCTCCT GGAGGAAACG CTTCCGCCCG CGGGACCCCC ACGGCCGCGC CTCGCTCAGC
    GCCGCGGCCG ACACGCTCCC GGCAACCTTC CGCGCGTCCA CCGTGGGGCC CGTGCCGCCT
    CCAGCATCCC CGCGGAAGAA GATCACGCCT GAAGCTCGCT CCCACTATCT CTACGGACAC
    ATGCTCTCCG CCTTCCGGGA CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025133598.1
    CDS487..4110
    Translation

    Target ORF information:

    RefSeq Version XM_025277813.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis PTPRF interacting protein alpha 4 (PPFIA4), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025277813.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGTGTGAAG TGATGCCCAC AATCAACGAG GGAGACCCCC TGGGGCCTCC CCATGGAGCC 
    GATGCTGACG CCAACTTTGA GCAGCTGATG GTGAACATGC TGGATGAGCG GGAGAAGTTG
    CTAGAGTCCC TTCGGGAGAG TCAGGAGACT TTGCTGGCGA CACAGAGCCG GCTCCAGGAT
    GCCCTGCATG AGCGAGACCA GCTCCAGCGC CACCTTAACT CTGCCCTCCC CCAGGAGTTT
    ACCACCTTAA CCCGGGAGCT GAGTATGTGT CGGGAGCAGC TTCTAGAGAG GGAGGAAGAG
    ATATCAGAGC TGAAAGCAGA GCGGAATAAC ACGAGGTTGC TTCTGGAACA TCTGGAGTGC
    CTGGTGTCCC GCCATGAGCG GTCACTGAGG ATGACTGTGG TGAAGCGTCA GGCCCAGTCA
    CCCTCAGGGG TCTCCAGTGA GGTGGAGGTG CTGAAGGCTC TCAAGTCACT GTTTGAGCAC
    CACAAGGCCC TGGATGAGAA GGTGCGAGAG CGGCTCCGGG TGGCCCTCGA GAGAGTGACC
    ACTTTGGAGG AACAGCTGGC GTGTGCCCAC CAGCAGGTGT CTTCCCTACA GCAGGGGTCA
    GGGGTTCGGG ATGGAGTGGC CGAAGAGGAG GGGACTGTGG AGCTGGGATC AAAAGGCCTG
    TGGAAGGAGG ACACAGGCCA GGTTGAGGAG CTGCAGGAGC TTCTGGAGAA GCAGAACTTT
    GAGTTGAGCC AGGCCCGGGA GCGACTGGTC ACCCTGACAG CAACTGTGGC TGAACTGGAG
    GAGGACCTGG GCACAGCCCG CCGGGACCTC ATCAAGTCGG AGGAACTGAG CAGCAAACAC
    CAGCGGGACC TCCGGGAAGC TCTAGCCCAG AAAGAGGACA TGGAGGAGCG GATCACCACC
    CTGGAGAAGC GCTACCTGGC TGCTCAGCGT GAGGCTACAT CAATACATGA CCTCAATGAC
    AAACTGGAGA ACGAGCTAGC CAATAGGGAA TCCTTGCATC GCCAGTGTGA GGAGAAAGCC
    CGATATCTGC AGGAGCTACT GGAGGTAGCC GAGCAGAAAC TTCAGCAGAC AATGCGCAAG
    GCCGAGACGC TGCCAGAGGT GGAGGCTGAG CTGGCCCAAA GAGTTGCGGC CCTCACCAAG
    GCTGAAGAGC GACATGGCAA CACTGAGGAG CACCTCCGAC AGCTGGAGGG ACAGCTGGAG
    GAGAAGAACC AAGAGCTGGC GCGGGTGCGC CAGCGGGAGA GGATGAATGA AGCCCACAAC
    AAGCGCCTGT CAGACACTGT GGACCGGCTG CTTATCGAGG CGAAGGAGCG CCTGCAGCTC
    CACCTCAGGG AGCGCATGGT GGCCCTGGAG GAGAAGAACA CATTGATCCA AGAGCTGGAG
    ACCTCCCAGC GGCAGATTGA AGAGCAGCAC CATCACAAGG GCCGCCTGTC TGAAGAGATT
    GAGAAACTGC GCCAAGAGGT GGACCAACTG AAGGGTCGAG GAGGACCATT TGTGGATGGC
    ATCCACTCCA GGGCGTACAC GGGCAGTGCA ATGGATGTGC GGTTCTCCCT GAGCACAACT
    GCCCACACAT CTCAAGGTCT GCGTCGTTGT TACTCAGCAC GGCGGGAACC GCCTGCCAAG
    GACTGGGAGC CATCTCCACG GCCTGCGGTG CTGGTCCCGA CAACCACCCC TGCCTTTGAC
    AGTGACCGTG AGATCTCTGA TGTGGATGAG GATGAACCAG GGGGTCTGGT GGGCTCCATG
    GACGTTGTCT CCCCCAGCAG CCACTCAGAC GCCCAGACCC TGGCCATGAT GCTGCAGGAG
    CAGCTGGATG CTATCAACGA GGAGATCAGG ATGATCCAGG AAGAGAAGGA GTCCACAGAG
    CTCCGTGCTG AGGAGCTGGA AACTCGAGTG ACTAGTGGCA GCATGGAGGC CCTAGACCTG
    ACCCAGCTGC ACAAACGCGG TTCCATCCCC ACCTCTCTGA CCGCCCTGTC CCTGGCCAGC
    GCGTCCCCTC CACTTAGCGG CCGTGCCACA CCTAAGCTCA CCTCCCGCAG TGCTGCCCAG
    GACCTGGATC GGATGGGGGT CATGACCTTG CCCAGTGACT TAAGAAAGCA TAGGAGGAAG
    CTGCTGTCAC CAGTGTCTTG GGAAGAGAAC CGAGAGGATA AAGCCACCAT AAAATGTGAG
    ACTTCCCCTC CTTCCTCACC CAGGACACTG CAGCTAGAGA AGCTTGGCCT CCCTGCCCTG
    AGCCAGGAAG AAGGCAAGAG TGCTTTGGAG GATCCAGGCA GCAACCCCAG CAGCAGCAAC
    AGCAGCCAGG ACTCCTTGCA CAAGGGTGCC AAGCGCAAGG GCATCAAATC ATCCATCGGC
    CGCCTGTTTG GGAAGAAGGA GAAAGGCAGG CTGATCCAGC CGAGCCGGGA TGGACCCACA
    GGCCATGTTG TACTACTAAC AGACTCTGAG CTCGGTCTGC AGGAGCCCAT GGTGCCTGGC
    AAGCTGGGAA CCCAGGCAGA AAAGGACCGG AGGCTGAAGA AGAAACACCA GCTGCTTGAA
    GATGCCCGGA GAAAAGGAAT GCCCTTTGCC CAGTGGGACG GCCCTACTGT GGTCTCCTGG
    CTAGAGCTCT GGGTGGGGAT GCCTGCTTGG TATGTGGCAG CCTGCCGGGC CAACGTCAAG
    AGTGGCGCCA TCATGTCAGC CCTATCGGAC ACAGAGATCC AGCGAGAAAT TGGCATCAGC
    AATGCCCTGC ACCGGCTCAA GCTCCGGCTG GCCATCCAGG AGATGGTGTC GCTGACCAGC
    CCCTCTGCCC CGCCCACCTC CAGGACTTCT TCTGGGAATG TCTGGGTCAC CCACGAAGAG
    ATGGAAACTC TGGCAACGTC CACTAAAACA GACAGTGAGG AGGGCAGCTG GGCTCAGACC
    CTGGTCTATG GGGATATGAA CCACGAGTGG ATTGGGAACC AGTGGCTCCC CAGCCTGGGG
    CTCCCCCAGT ACCGCAGCTA CTTCATGGAG TGCCTCGTGG ACGCTCGCAT GCTGGATCAC
    CTCACCAAGA AGGACTTGCG GGTCCACCTG AAGATGGTGG ACAGCTCCCA CCGAACCAGT
    CTTCAGTATG GCATCATGTG CCTGAAGAGG CTGAATTATG ACCGGAAGGA GCTGGAGAAG
    CGGCGGGAGG AGAGCCAGCA TGAGATCAAG GATGTGCTGG TCTGGACCAA TGACCAGGTA
    GTTCATTGGG TCCAGTCTAT TGGGCTCCGA GACTACGCAG GAAACCTCCA TGAGAGTGGT
    GTGCATGGCG CTTTGCTGGC CCTGGATGAG AACTTTGACC ACAACACACT GGCCCTGGTT
    CTCCAGATAC CCACACAAAA TACCCAGGCA CGCCAAGTGT TGGAGAGAGA GTTCAACAAC
    CTGTTGGCTT TGGGCACAGA CCGGAAGCTG GATGATGGGG AAGACAAGGC GCTACGCCGC
    GCGCCCTCCT GGAGGAAACG CTTCCGCCCG CGGGACCCCC ACGGCCGCGC CTCGCTCAGC
    GCCGCGGCCG ACACGCTCCC GGCAACCTTC CGCGCGTCCA CCGTGGGGCC CGTGCCGCCT
    CCAGCATCCC CGCGGAAGAA GATCACGCCT GAAGCTCGCT CCCACTATCT CTACGGACAC
    ATGCTCTCCG CCTTCCGGGA CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu41797
    Clone ID Related Accession (Same CDS sequence) XM_025277814.1
    Accession Version XM_025277814.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3597bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product liprin-alpha-4 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_020228958.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGTGTGAAG TGATGCCCAC AATCAACGAG GGAGACCCCC TGGGGCCTCC CCATGGAGCC 
    GATGCTGACG CCAACTTTGA GCAGCTGATG GTGAACATGC TGGATGAGCG GGAGAAGTTG
    CTAGAGTCCC TTCGGGAGAG TCAGGAGACT TTGCTGGCGA CACAGAGCCG GCTCCAGGAT
    GCCCTGCATG AGCGAGACCA GCTCCAGCGC CACCTTAACT CTGCCCTCCC CCAGGAGTTT
    ACCACCTTAA CCCGGGAGCT GAGTATGTGT CGGGAGCAGC TTCTAGAGAG GGAGGAAGAG
    ATATCAGAGC TGAAAGCAGA GCGGAATAAC ACGAGGTTGC TTCTGGAACA TCTGGAGTGC
    CTGGTGTCCC GCCATGAGCG GTCACTGAGG ATGACTGTGG TGAAGCGTCA GGCCCAGTCA
    CCCTCAGGGG TCTCCAGTGA GGTGGAGGTG CTGAAGGCTC TCAAGTCACT GTTTGAGCAC
    CACAAGGCCC TGGATGAGAA GGTGCGAGAG CGGCTCCGGG TGGCCCTCGA GAGAGTGACC
    ACTTTGGAGG AACAGCTGGC GTGTGCCCAC CAGCAGGTGT CTTCCCTACA GCAGGGGTCA
    GGGGTTCGGG ATGGAGTGGC CGAAGAGGAG GGGACTGTGG AGCTGGGATC AAAAGGCCTG
    TGGAAGGAGG ACACAGGCCA GGTTGAGGAG CTGCAGGAGC TTCTGGAGAA GCAGAACTTT
    GAGTTGAGCC AGGCCCGGGA GCGACTGGTC ACCCTGACAG CAACTGTGGC TGAACTGGAG
    GAGGACCTGG GCACAGCCCG CCGGGACCTC ATCAAGTCGG AGGAACTGAG CAGCAAACAC
    CAGCGGGACC TCCGGGAAGC TCTAGCCCAG AAAGAGGACA TGGAGGAGCG GATCACCACC
    CTGGAGAAGC GCTACCTGGC TGCTCAGCGT GAGGCTACAT CAATACATGA CCTCAATGAC
    AAACTGGAGA ACGAGCTAGC CAATAGGGAA TCCTTGCATC GCCAGTGTGA GGAGAAAGCC
    CGATATCTGC AGGAGCTACT GGAGGTAGCC GAGCAGAAAC TTCAGCAGAC AATGCGCAAG
    GCCGAGACGC TGCCAGAGGT GGAGGCTGAG CTGGCCCAAA GAGTTGCGGC CCTCACCAAG
    GCTGAAGAGC GACATGGCAA CACTGAGGAG CACCTCCGAC AGCTGGAGGG ACAGCTGGAG
    GAGAAGAACC AAGAGCTGGC GCGGGTGCGC CAGCGGGAGA GGATGAATGA AGCCCACAAC
    AAGCGCCTGT CAGACACTGT GGACCGGCTG CTTATCGAGG CGAAGGAGCG CCTGCAGCTC
    CACCTCAGGG AGCGCATGGT GGCCCTGGAG GAGAAGAACA CATTGATCCA AGAGCTGGAG
    ACCTCCCAGC GGCAGATTGA AGAGCAGCAC CATCACAAGG GCCGCCTGTC TGAAGAGATT
    GAGAAACTGC GCCAAGAGGT GGACCAACTG AAGGGTCGAG GAGGACCATT TGTGGATGGC
    ATCCACTCCA GGGCGTACAC GGGCAGTGCA ATGGATGTGC GGTTCTCCCT GAGCACAACT
    GCCCACACAT CTCAAGGTCT GCGTCGTTGT TACTCAGCAC GGCGGGAACC GCCTGCCAAG
    GACTGGGAGC CATCTCCACG GCCTGCGGTG CTGGTCCCGA CAACCACCCC TGCCTTTGAC
    AGTGACCGTG AGATCTCTGA TGTGGATGAG GATGAACCAG GGGGTCTGGT GGGCTCCATG
    GACGTTGTCT CCCCCAGCAG CCACTCAGAC GCCCAGACCC TGGCCATGAT GCTGCAGGAG
    CAGCTGGATG CTATCAACGA GGAGATCAGG ATGATCCAGG AAGAGAAGGA GTCCACAGAG
    CTCCGTGCTG AGGAGCTGGA AACTCGAGTG ACTAGTGGCA GCATGGAGGC CCTAGACCTG
    ACCCAGCTGC ACAAACGCGG TTCCATCCCC ACCTCTCTGA CCGCCCTGTC CCTGGCCAGC
    GCGTCCCCTC CACTTAGCGG CCGTGCCACA CCTAAGCTCA CCTCCCGCAG TGCTGCCCAG
    GACCTGGATC GGATGGGGGT CATGACCTTG CCCAGTGACT TAAGAAAGCA TAGGAGGAAG
    CTGCTGTCAC CAGTGTCTTG GGAAGAGAAC CGAGAGGATA AAGCCACCAT AAAATGTGAG
    ACTTCCCCTC CTTCCTCACC CAGGACACTG CAGCTAGAGA AGCTTGGCCT CCCTGCCCTG
    AGCCAGGAAG AAGGCAAGAG TGCTTTGGAG GATCCAGGCA GCAACCCCAG CAGCAGCAAC
    AGCAGCCAGG ACTCCTTGCA CAAGGGTGCC AAGCGCAAGG GCATCAAATC ATCCATCGGC
    CGCCTGTTTG GGAAGAAGGA GAAAGGCAGG CTGATCCAGC CGAGCCGGGA TGGACCCACA
    GGCCATGTTG TACTACTAAC AGACTCTGAG CTCGGTCTGC AGGAGCCCAT GGTGCCTGGC
    AAGCTGGGAA CCCAGGCAGA AAAGGACCGG AGGCTGAAGA AGAAACACCA GCTGCTTGAA
    GATGCCCGGA GAAAAGGAAT GCCCTTTGCC CAGTGGGACG GCCCTACTGT GGTCTCCTGG
    CTAGAGCTCT GGGTGGGGAT GCCTGCTTGG TATGTGGCAG CCTGCCGGGC CAACGTCAAG
    AGTGGCGCCA TCATGTCAGC CCTATCGGAC ACAGAGATCC AGCGAGAAAT TGGCATCAGC
    AATGCCCTGC ACCGGCTCAA GCTCCGGCTG GCCATCCAGG AGATGGTGTC GCTGACCAGC
    CCCTCTGCCC CGCCCACCTC CAGGACTTCT TCTGGGAATG TCTGGGTCAC CCACGAAGAG
    ATGGAAACTC TGGCAACGTC CACTAAAACA ACCCTGGTCT ATGGGGATAT GAACCACGAG
    TGGATTGGGA ACCAGTGGCT CCCCAGCCTG GGGCTCCCCC AGTACCGCAG CTACTTCATG
    GAGTGCCTCG TGGACGCTCG CATGCTGGAT CACCTCACCA AGAAGGACTT GCGGGTCCAC
    CTGAAGATGG TGGACAGCTC CCACCGAACC AGTCTTCAGT ATGGCATCAT GTGCCTGAAG
    AGGCTGAATT ATGACCGGAA GGAGCTGGAG AAGCGGCGGG AGGAGAGCCA GCATGAGATC
    AAGGATGTGC TGGTCTGGAC CAATGACCAG GTAGTTCATT GGGTCCAGTC TATTGGGCTC
    CGAGACTACG CAGGAAACCT CCATGAGAGT GGTGTGCATG GCGCTTTGCT GGCCCTGGAT
    GAGAACTTTG ACCACAACAC ACTGGCCCTG GTTCTCCAGA TACCCACACA AAATACCCAG
    GCACGCCAAG TGTTGGAGAG AGAGTTCAAC AACCTGTTGG CTTTGGGCAC AGACCGGAAG
    CTGGATGATG GGGAAGACAA GGCGCTACGC CGCGCGCCCT CCTGGAGGAA ACGCTTCCGC
    CCGCGGGACC CCCACGGCCG CGCCTCGCTC AGCGCCGCGG CCGACACGCT CCCGGCAACC
    TTCCGCGCGT CCACCGTGGG GCCCGTGCCG CCTCCAGCAT CCCCGCGGAA GAAGATCACG
    CCTGAAGCTC GCTCCCACTA TCTCTACGGA CACATGCTCT CCGCCTTCCG GGACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025133599.1
    CDS487..4083
    Translation

    Target ORF information:

    RefSeq Version XM_025277814.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis PTPRF interacting protein alpha 4 (PPFIA4), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025277814.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGTGTGAAG TGATGCCCAC AATCAACGAG GGAGACCCCC TGGGGCCTCC CCATGGAGCC 
    GATGCTGACG CCAACTTTGA GCAGCTGATG GTGAACATGC TGGATGAGCG GGAGAAGTTG
    CTAGAGTCCC TTCGGGAGAG TCAGGAGACT TTGCTGGCGA CACAGAGCCG GCTCCAGGAT
    GCCCTGCATG AGCGAGACCA GCTCCAGCGC CACCTTAACT CTGCCCTCCC CCAGGAGTTT
    ACCACCTTAA CCCGGGAGCT GAGTATGTGT CGGGAGCAGC TTCTAGAGAG GGAGGAAGAG
    ATATCAGAGC TGAAAGCAGA GCGGAATAAC ACGAGGTTGC TTCTGGAACA TCTGGAGTGC
    CTGGTGTCCC GCCATGAGCG GTCACTGAGG ATGACTGTGG TGAAGCGTCA GGCCCAGTCA
    CCCTCAGGGG TCTCCAGTGA GGTGGAGGTG CTGAAGGCTC TCAAGTCACT GTTTGAGCAC
    CACAAGGCCC TGGATGAGAA GGTGCGAGAG CGGCTCCGGG TGGCCCTCGA GAGAGTGACC
    ACTTTGGAGG AACAGCTGGC GTGTGCCCAC CAGCAGGTGT CTTCCCTACA GCAGGGGTCA
    GGGGTTCGGG ATGGAGTGGC CGAAGAGGAG GGGACTGTGG AGCTGGGATC AAAAGGCCTG
    TGGAAGGAGG ACACAGGCCA GGTTGAGGAG CTGCAGGAGC TTCTGGAGAA GCAGAACTTT
    GAGTTGAGCC AGGCCCGGGA GCGACTGGTC ACCCTGACAG CAACTGTGGC TGAACTGGAG
    GAGGACCTGG GCACAGCCCG CCGGGACCTC ATCAAGTCGG AGGAACTGAG CAGCAAACAC
    CAGCGGGACC TCCGGGAAGC TCTAGCCCAG AAAGAGGACA TGGAGGAGCG GATCACCACC
    CTGGAGAAGC GCTACCTGGC TGCTCAGCGT GAGGCTACAT CAATACATGA CCTCAATGAC
    AAACTGGAGA ACGAGCTAGC CAATAGGGAA TCCTTGCATC GCCAGTGTGA GGAGAAAGCC
    CGATATCTGC AGGAGCTACT GGAGGTAGCC GAGCAGAAAC TTCAGCAGAC AATGCGCAAG
    GCCGAGACGC TGCCAGAGGT GGAGGCTGAG CTGGCCCAAA GAGTTGCGGC CCTCACCAAG
    GCTGAAGAGC GACATGGCAA CACTGAGGAG CACCTCCGAC AGCTGGAGGG ACAGCTGGAG
    GAGAAGAACC AAGAGCTGGC GCGGGTGCGC CAGCGGGAGA GGATGAATGA AGCCCACAAC
    AAGCGCCTGT CAGACACTGT GGACCGGCTG CTTATCGAGG CGAAGGAGCG CCTGCAGCTC
    CACCTCAGGG AGCGCATGGT GGCCCTGGAG GAGAAGAACA CATTGATCCA AGAGCTGGAG
    ACCTCCCAGC GGCAGATTGA AGAGCAGCAC CATCACAAGG GCCGCCTGTC TGAAGAGATT
    GAGAAACTGC GCCAAGAGGT GGACCAACTG AAGGGTCGAG GAGGACCATT TGTGGATGGC
    ATCCACTCCA GGGCGTACAC GGGCAGTGCA ATGGATGTGC GGTTCTCCCT GAGCACAACT
    GCCCACACAT CTCAAGGTCT GCGTCGTTGT TACTCAGCAC GGCGGGAACC GCCTGCCAAG
    GACTGGGAGC CATCTCCACG GCCTGCGGTG CTGGTCCCGA CAACCACCCC TGCCTTTGAC
    AGTGACCGTG AGATCTCTGA TGTGGATGAG GATGAACCAG GGGGTCTGGT GGGCTCCATG
    GACGTTGTCT CCCCCAGCAG CCACTCAGAC GCCCAGACCC TGGCCATGAT GCTGCAGGAG
    CAGCTGGATG CTATCAACGA GGAGATCAGG ATGATCCAGG AAGAGAAGGA GTCCACAGAG
    CTCCGTGCTG AGGAGCTGGA AACTCGAGTG ACTAGTGGCA GCATGGAGGC CCTAGACCTG
    ACCCAGCTGC ACAAACGCGG TTCCATCCCC ACCTCTCTGA CCGCCCTGTC CCTGGCCAGC
    GCGTCCCCTC CACTTAGCGG CCGTGCCACA CCTAAGCTCA CCTCCCGCAG TGCTGCCCAG
    GACCTGGATC GGATGGGGGT CATGACCTTG CCCAGTGACT TAAGAAAGCA TAGGAGGAAG
    CTGCTGTCAC CAGTGTCTTG GGAAGAGAAC CGAGAGGATA AAGCCACCAT AAAATGTGAG
    ACTTCCCCTC CTTCCTCACC CAGGACACTG CAGCTAGAGA AGCTTGGCCT CCCTGCCCTG
    AGCCAGGAAG AAGGCAAGAG TGCTTTGGAG GATCCAGGCA GCAACCCCAG CAGCAGCAAC
    AGCAGCCAGG ACTCCTTGCA CAAGGGTGCC AAGCGCAAGG GCATCAAATC ATCCATCGGC
    CGCCTGTTTG GGAAGAAGGA GAAAGGCAGG CTGATCCAGC CGAGCCGGGA TGGACCCACA
    GGCCATGTTG TACTACTAAC AGACTCTGAG CTCGGTCTGC AGGAGCCCAT GGTGCCTGGC
    AAGCTGGGAA CCCAGGCAGA AAAGGACCGG AGGCTGAAGA AGAAACACCA GCTGCTTGAA
    GATGCCCGGA GAAAAGGAAT GCCCTTTGCC CAGTGGGACG GCCCTACTGT GGTCTCCTGG
    CTAGAGCTCT GGGTGGGGAT GCCTGCTTGG TATGTGGCAG CCTGCCGGGC CAACGTCAAG
    AGTGGCGCCA TCATGTCAGC CCTATCGGAC ACAGAGATCC AGCGAGAAAT TGGCATCAGC
    AATGCCCTGC ACCGGCTCAA GCTCCGGCTG GCCATCCAGG AGATGGTGTC GCTGACCAGC
    CCCTCTGCCC CGCCCACCTC CAGGACTTCT TCTGGGAATG TCTGGGTCAC CCACGAAGAG
    ATGGAAACTC TGGCAACGTC CACTAAAACA ACCCTGGTCT ATGGGGATAT GAACCACGAG
    TGGATTGGGA ACCAGTGGCT CCCCAGCCTG GGGCTCCCCC AGTACCGCAG CTACTTCATG
    GAGTGCCTCG TGGACGCTCG CATGCTGGAT CACCTCACCA AGAAGGACTT GCGGGTCCAC
    CTGAAGATGG TGGACAGCTC CCACCGAACC AGTCTTCAGT ATGGCATCAT GTGCCTGAAG
    AGGCTGAATT ATGACCGGAA GGAGCTGGAG AAGCGGCGGG AGGAGAGCCA GCATGAGATC
    AAGGATGTGC TGGTCTGGAC CAATGACCAG GTAGTTCATT GGGTCCAGTC TATTGGGCTC
    CGAGACTACG CAGGAAACCT CCATGAGAGT GGTGTGCATG GCGCTTTGCT GGCCCTGGAT
    GAGAACTTTG ACCACAACAC ACTGGCCCTG GTTCTCCAGA TACCCACACA AAATACCCAG
    GCACGCCAAG TGTTGGAGAG AGAGTTCAAC AACCTGTTGG CTTTGGGCAC AGACCGGAAG
    CTGGATGATG GGGAAGACAA GGCGCTACGC CGCGCGCCCT CCTGGAGGAA ACGCTTCCGC
    CCGCGGGACC CCCACGGCCG CGCCTCGCTC AGCGCCGCGG CCGACACGCT CCCGGCAACC
    TTCCGCGCGT CCACCGTGGG GCCCGTGCCG CCTCCAGCAT CCCCGCGGAA GAAGATCACG
    CCTGAAGCTC GCTCCCACTA TCTCTACGGA CACATGCTCT CCGCCTTCCG GGACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu41798
    Clone ID Related Accession (Same CDS sequence) XM_025277815.1
    Accession Version XM_025277815.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3570bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product liprin-alpha-4 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_020228958.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGAGGCAG GGCTCCCTTG GGCCCTAAAA CTTGACTGGC GTTGGGTAAA AATCTGGCTC 
    CCCGCCACCT CACGAGATAC TCTCCCGGCC GAGCCAAGAT GGCGCGCAGC CCCAAGTGGC
    TCCACGTCAC GAGGGAGGGA GCGCCGGGGC GGGGGCGTGG CCTCGAGGGG GCCGGCTAGC
    CCCGCCCTTG AGGTGGGGCC GGGGGCCGTC CGCAGAAGGC GAGGGCGGGG CGCTTCGGCT
    CGGGAAGGCT GCGGCTGCCT CGGCGCCGGC TCCGCTCGCG AGGACAAGTC CGGCGCCTCC
    AGGCCGGGCC GGCTTCCTGC GCAGAGCCAA GCTGCCCGGC CTTCGGAGTT TACCACCTTA
    ACCCGGGAGC TGAGTATGTG TCGGGAGCAG CTTCTAGAGA GGGAGGAAGA GATATCAGAG
    CTGAAAGCAG AGCGGAATAA CACGAGGTTG CTTCTGGAAC ATCTGGAGTG CCTGGTGTCC
    CGCCATGAGC GGTCACTGAG GATGACTGTG GTGAAGCGTC AGGCCCAGTC ACCCTCAGGG
    GTCTCCAGTG AGGTGGAGGT GCTGAAGGCT CTCAAGTCAC TGTTTGAGCA CCACAAGGCC
    CTGGATGAGA AGGTGCGAGA GCGGCTCCGG GTGGCCCTCG AGAGAGTGAC CACTTTGGAG
    GAACAGCTGG CGTGTGCCCA CCAGCAGGTG TCTTCCCTAC AGCAGGGGTC AGGGGTTCGG
    GATGGAGTGG CCGAAGAGGA GGGGACTGTG GAGCTGGGAT CAAAAGGCCT GTGGAAGGAG
    GACACAGGCC AGGTTGAGGA GCTGCAGGAG CTTCTGGAGA AGCAGAACTT TGAGTTGAGC
    CAGGCCCGGG AGCGACTGGT CACCCTGACA GCAACTGTGG CTGAACTGGA GGAGGACCTG
    GGCACAGCCC GCCGGGACCT CATCAAGTCG GAGGAACTGA GCAGCAAACA CCAGCGGGAC
    CTCCGGGAAG CTCTAGCCCA GAAAGAGGAC ATGGAGGAGC GGATCACCAC CCTGGAGAAG
    CGCTACCTGG CTGCTCAGCG TGAGGCTACA TCAATACATG ACCTCAATGA CAAACTGGAG
    AACGAGCTAG CCAATAGGGA ATCCTTGCAT CGCCAGTGTG AGGAGAAAGC CCGATATCTG
    CAGGAGCTAC TGGAGGTAGC CGAGCAGAAA CTTCAGCAGA CAATGCGCAA GGCCGAGACG
    CTGCCAGAGG TGGAGGCTGA GCTGGCCCAA AGAGTTGCGG CCCTCACCAA GGCTGAAGAG
    CGACATGGCA ACACTGAGGA GCACCTCCGA CAGCTGGAGG GACAGCTGGA GGAGAAGAAC
    CAAGAGCTGG CGCGGGTGCG CCAGCGGGAG AGGATGAATG AAGCCCACAA CAAGCGCCTG
    TCAGACACTG TGGACCGGCT GCTTATCGAG GCGAAGGAGC GCCTGCAGCT CCACCTCAGG
    GAGCGCATGG TGGCCCTGGA GGAGAAGAAC ACATTGATCC AAGAGCTGGA GACCTCCCAG
    CGGCAGATTG AAGAGCAGCA CCATCACAAG GGCCGCCTGT CTGAAGAGAT TGAGAAACTG
    CGCCAAGAGG TGGACCAACT GAAGGGTCGA GGAGGACCAT TTGTGGATGG CATCCACTCC
    AGGGCGTACA CGGGCAGTGC AATGGATGTG CGGTTCTCCC TGAGCACAAC TGCCCACACA
    TCTCAAGGTC TGCGTCGTTG TTACTCAGCA CGGCGGGAAC CGCCTGCCAA GGACTGGGAG
    CCATCTCCAC GGCCTGCGGT GCTGGTCCCG ACAACCACCC CTGCCTTTGA CAGTGACCGT
    GAGATCTCTG ATGTGGATGA GGATGAACCA GGGGGTCTGG TGGGCTCCAT GGACGTTGTC
    TCCCCCAGCA GCCACTCAGA CGCCCAGACC CTGGCCATGA TGCTGCAGGA GCAGCTGGAT
    GCTATCAACG AGGAGATCAG GATGATCCAG GAAGAGAAGG AGTCCACAGA GCTCCGTGCT
    GAGGAGCTGG AAACTCGAGT GACTAGTGGC AGCATGGAGG CCCTAGACCT GACCCAGCTG
    CACAAACGCG GTTCCATCCC CACCTCTCTG ACCGCCCTGT CCCTGGCCAG CGCGTCCCCT
    CCACTTAGCG GCCGTGCCAC ACCTAAGCTC ACCTCCCGCA GTGCTGCCCA GGACCTGGAT
    CGGATGGGGG TCATGACCTT GCCCAGTGAC TTAAGAAAGC ATAGGAGGAA GCTGCTGTCA
    CCAGTGTCTT GGGAAGAGAA CCGAGAGGAT AAAGCCACCA TAAAATGTGA GACTTCCCCT
    CCTTCCTCAC CCAGGACACT GCAGCTAGAG AAGCTTGGCC TCCCTGCCCT GAGCCAGGAA
    GAAGGCAAGA GTGCTTTGGA GGATCCAGGC AGCAACCCCA GCAGCAGCAA CAGCAGCCAG
    GACTCCTTGC ACAAGGGTGC CAAGCGCAAG GGCATCAAAT CATCCATCGG CCGCCTGTTT
    GGGAAGAAGG AGAAAGGCAG GCTGATCCAG CCGAGCCGGG ATGGACCCAC AGGCCATGTT
    GTACTACTAA CAGACTCTGA GCTCGGTCTG CAGGAGCCCA TGGTGCCTGG CAAGCTGGGA
    ACCCAGGCAG AAAAGGACCG GAGGCTGAAG AAGAAACACC AGCTGCTTGA AGATGCCCGG
    AGAAAAGGAA TGCCCTTTGC CCAGTGGGAC GGCCCTACTG TGGTCTCCTG GCTAGAGCTC
    TGGGTGGGGA TGCCTGCTTG GTATGTGGCA GCCTGCCGGG CCAACGTCAA GAGTGGCGCC
    ATCATGTCAG CCCTATCGGA CACAGAGATC CAGCGAGAAA TTGGCATCAG CAATGCCCTG
    CACCGGCTCA AGCTCCGGCT GGCCATCCAG GAGATGGTGT CGCTGACCAG CCCCTCTGCC
    CCGCCCACCT CCAGGACTTC TTCTGGGAAT GTCTGGGTCA CCCACGAAGA GATGGAAACT
    CTGGCAACGT CCACTAAAAC AGACAGTGAG GAGGGCAGCT GGGCTCAGAC CCTGGTCTAT
    GGGGATATGA ACCACGAGTG GATTGGGAAC CAGTGGCTCC CCAGCCTGGG GCTCCCCCAG
    TACCGCAGCT ACTTCATGGA GTGCCTCGTG GACGCTCGCA TGCTGGATCA CCTCACCAAG
    AAGGACTTGC GGGTCCACCT GAAGATGGTG GACAGCTCCC ACCGAACCAG TCTTCAGTAT
    GGCATCATGT GCCTGAAGAG GCTGAATTAT GACCGGAAGG AGCTGGAGAA GCGGCGGGAG
    GAGAGCCAGC ATGAGATCAA GGATGTGCTG GTCTGGACCA ATGACCAGGT AGTTCATTGG
    GTCCAGTCTA TTGGGCTCCG AGACTACGCA GGAAACCTCC ATGAGAGTGG TGTGCATGGC
    GCTTTGCTGG CCCTGGATGA GAACTTTGAC CACAACACAC TGGCCCTGGT TCTCCAGATA
    CCCACACAAA ATACCCAGGC ACGCCAAGTG TTGGAGAGAG AGTTCAACAA CCTGTTGGCT
    TTGGGCACAG ACCGGAAGCT GGATGATGGA AAGGTGCCCC TAGAACAGGC ACTACCTGCC
    CTCCCAGGCA GGGACGAAGG GCGGCCGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025133600.1
    CDS564..4133
    Translation

    Target ORF information:

    RefSeq Version XM_025277815.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis PTPRF interacting protein alpha 4 (PPFIA4), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025277815.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGAGGCAG GGCTCCCTTG GGCCCTAAAA CTTGACTGGC GTTGGGTAAA AATCTGGCTC 
    CCCGCCACCT CACGAGATAC TCTCCCGGCC GAGCCAAGAT GGCGCGCAGC CCCAAGTGGC
    TCCACGTCAC GAGGGAGGGA GCGCCGGGGC GGGGGCGTGG CCTCGAGGGG GCCGGCTAGC
    CCCGCCCTTG AGGTGGGGCC GGGGGCCGTC CGCAGAAGGC GAGGGCGGGG CGCTTCGGCT
    CGGGAAGGCT GCGGCTGCCT CGGCGCCGGC TCCGCTCGCG AGGACAAGTC CGGCGCCTCC
    AGGCCGGGCC GGCTTCCTGC GCAGAGCCAA GCTGCCCGGC CTTCGGAGTT TACCACCTTA
    ACCCGGGAGC TGAGTATGTG TCGGGAGCAG CTTCTAGAGA GGGAGGAAGA GATATCAGAG
    CTGAAAGCAG AGCGGAATAA CACGAGGTTG CTTCTGGAAC ATCTGGAGTG CCTGGTGTCC
    CGCCATGAGC GGTCACTGAG GATGACTGTG GTGAAGCGTC AGGCCCAGTC ACCCTCAGGG
    GTCTCCAGTG AGGTGGAGGT GCTGAAGGCT CTCAAGTCAC TGTTTGAGCA CCACAAGGCC
    CTGGATGAGA AGGTGCGAGA GCGGCTCCGG GTGGCCCTCG AGAGAGTGAC CACTTTGGAG
    GAACAGCTGG CGTGTGCCCA CCAGCAGGTG TCTTCCCTAC AGCAGGGGTC AGGGGTTCGG
    GATGGAGTGG CCGAAGAGGA GGGGACTGTG GAGCTGGGAT CAAAAGGCCT GTGGAAGGAG
    GACACAGGCC AGGTTGAGGA GCTGCAGGAG CTTCTGGAGA AGCAGAACTT TGAGTTGAGC
    CAGGCCCGGG AGCGACTGGT CACCCTGACA GCAACTGTGG CTGAACTGGA GGAGGACCTG
    GGCACAGCCC GCCGGGACCT CATCAAGTCG GAGGAACTGA GCAGCAAACA CCAGCGGGAC
    CTCCGGGAAG CTCTAGCCCA GAAAGAGGAC ATGGAGGAGC GGATCACCAC CCTGGAGAAG
    CGCTACCTGG CTGCTCAGCG TGAGGCTACA TCAATACATG ACCTCAATGA CAAACTGGAG
    AACGAGCTAG CCAATAGGGA ATCCTTGCAT CGCCAGTGTG AGGAGAAAGC CCGATATCTG
    CAGGAGCTAC TGGAGGTAGC CGAGCAGAAA CTTCAGCAGA CAATGCGCAA GGCCGAGACG
    CTGCCAGAGG TGGAGGCTGA GCTGGCCCAA AGAGTTGCGG CCCTCACCAA GGCTGAAGAG
    CGACATGGCA ACACTGAGGA GCACCTCCGA CAGCTGGAGG GACAGCTGGA GGAGAAGAAC
    CAAGAGCTGG CGCGGGTGCG CCAGCGGGAG AGGATGAATG AAGCCCACAA CAAGCGCCTG
    TCAGACACTG TGGACCGGCT GCTTATCGAG GCGAAGGAGC GCCTGCAGCT CCACCTCAGG
    GAGCGCATGG TGGCCCTGGA GGAGAAGAAC ACATTGATCC AAGAGCTGGA GACCTCCCAG
    CGGCAGATTG AAGAGCAGCA CCATCACAAG GGCCGCCTGT CTGAAGAGAT TGAGAAACTG
    CGCCAAGAGG TGGACCAACT GAAGGGTCGA GGAGGACCAT TTGTGGATGG CATCCACTCC
    AGGGCGTACA CGGGCAGTGC AATGGATGTG CGGTTCTCCC TGAGCACAAC TGCCCACACA
    TCTCAAGGTC TGCGTCGTTG TTACTCAGCA CGGCGGGAAC CGCCTGCCAA GGACTGGGAG
    CCATCTCCAC GGCCTGCGGT GCTGGTCCCG ACAACCACCC CTGCCTTTGA CAGTGACCGT
    GAGATCTCTG ATGTGGATGA GGATGAACCA GGGGGTCTGG TGGGCTCCAT GGACGTTGTC
    TCCCCCAGCA GCCACTCAGA CGCCCAGACC CTGGCCATGA TGCTGCAGGA GCAGCTGGAT
    GCTATCAACG AGGAGATCAG GATGATCCAG GAAGAGAAGG AGTCCACAGA GCTCCGTGCT
    GAGGAGCTGG AAACTCGAGT GACTAGTGGC AGCATGGAGG CCCTAGACCT GACCCAGCTG
    CACAAACGCG GTTCCATCCC CACCTCTCTG ACCGCCCTGT CCCTGGCCAG CGCGTCCCCT
    CCACTTAGCG GCCGTGCCAC ACCTAAGCTC ACCTCCCGCA GTGCTGCCCA GGACCTGGAT
    CGGATGGGGG TCATGACCTT GCCCAGTGAC TTAAGAAAGC ATAGGAGGAA GCTGCTGTCA
    CCAGTGTCTT GGGAAGAGAA CCGAGAGGAT AAAGCCACCA TAAAATGTGA GACTTCCCCT
    CCTTCCTCAC CCAGGACACT GCAGCTAGAG AAGCTTGGCC TCCCTGCCCT GAGCCAGGAA
    GAAGGCAAGA GTGCTTTGGA GGATCCAGGC AGCAACCCCA GCAGCAGCAA CAGCAGCCAG
    GACTCCTTGC ACAAGGGTGC CAAGCGCAAG GGCATCAAAT CATCCATCGG CCGCCTGTTT
    GGGAAGAAGG AGAAAGGCAG GCTGATCCAG CCGAGCCGGG ATGGACCCAC AGGCCATGTT
    GTACTACTAA CAGACTCTGA GCTCGGTCTG CAGGAGCCCA TGGTGCCTGG CAAGCTGGGA
    ACCCAGGCAG AAAAGGACCG GAGGCTGAAG AAGAAACACC AGCTGCTTGA AGATGCCCGG
    AGAAAAGGAA TGCCCTTTGC CCAGTGGGAC GGCCCTACTG TGGTCTCCTG GCTAGAGCTC
    TGGGTGGGGA TGCCTGCTTG GTATGTGGCA GCCTGCCGGG CCAACGTCAA GAGTGGCGCC
    ATCATGTCAG CCCTATCGGA CACAGAGATC CAGCGAGAAA TTGGCATCAG CAATGCCCTG
    CACCGGCTCA AGCTCCGGCT GGCCATCCAG GAGATGGTGT CGCTGACCAG CCCCTCTGCC
    CCGCCCACCT CCAGGACTTC TTCTGGGAAT GTCTGGGTCA CCCACGAAGA GATGGAAACT
    CTGGCAACGT CCACTAAAAC AGACAGTGAG GAGGGCAGCT GGGCTCAGAC CCTGGTCTAT
    GGGGATATGA ACCACGAGTG GATTGGGAAC CAGTGGCTCC CCAGCCTGGG GCTCCCCCAG
    TACCGCAGCT ACTTCATGGA GTGCCTCGTG GACGCTCGCA TGCTGGATCA CCTCACCAAG
    AAGGACTTGC GGGTCCACCT GAAGATGGTG GACAGCTCCC ACCGAACCAG TCTTCAGTAT
    GGCATCATGT GCCTGAAGAG GCTGAATTAT GACCGGAAGG AGCTGGAGAA GCGGCGGGAG
    GAGAGCCAGC ATGAGATCAA GGATGTGCTG GTCTGGACCA ATGACCAGGT AGTTCATTGG
    GTCCAGTCTA TTGGGCTCCG AGACTACGCA GGAAACCTCC ATGAGAGTGG TGTGCATGGC
    GCTTTGCTGG CCCTGGATGA GAACTTTGAC CACAACACAC TGGCCCTGGT TCTCCAGATA
    CCCACACAAA ATACCCAGGC ACGCCAAGTG TTGGAGAGAG AGTTCAACAA CCTGTTGGCT
    TTGGGCACAG ACCGGAAGCT GGATGATGGA AAGGTGCCCC TAGAACAGGC ACTACCTGCC
    CTCCCAGGCA GGGACGAAGG GCGGCCGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu41799
    Clone ID Related Accession (Same CDS sequence) XM_025277817.1 , XM_025277816.1
    Accession Version XM_025277817.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3519bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product liprin-alpha-4 isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_020228958.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGTGTCGGG AGCAGCTTCT AGAGAGGGAG GAAGAGATAT CAGAGCTGAA AGCAGAGCGG 
    AATAACACGA GGTTGCTTCT GGAACATCTG GAGTGCCTGG TGTCCCGCCA TGAGCGGTCA
    CTGAGGATGA CTGTGGTGAA GCGTCAGGCC CAGTCACCCT CAGGGGTCTC CAGTGAGGTG
    GAGGTGCTGA AGGCTCTCAA GTCACTGTTT GAGCACCACA AGGCCCTGGA TGAGAAGGTG
    CGAGAGCGGC TCCGGGTGGC CCTCGAGAGA GTGACCACTT TGGAGGAACA GCTGGCGTGT
    GCCCACCAGC AGGTGTCTTC CCTACAGCAG GGGTCAGGGG TTCGGGATGG AGTGGCCGAA
    GAGGAGGGGA CTGTGGAGCT GGGATCAAAA GGCCTGTGGA AGGAGGACAC AGGCCAGGTT
    GAGGAGCTGC AGGAGCTTCT GGAGAAGCAG AACTTTGAGT TGAGCCAGGC CCGGGAGCGA
    CTGGTCACCC TGACAGCAAC TGTGGCTGAA CTGGAGGAGG ACCTGGGCAC AGCCCGCCGG
    GACCTCATCA AGTCGGAGGA ACTGAGCAGC AAACACCAGC GGGACCTCCG GGAAGCTCTA
    GCCCAGAAAG AGGACATGGA GGAGCGGATC ACCACCCTGG AGAAGCGCTA CCTGGCTGCT
    CAGCGTGAGG CTACATCAAT ACATGACCTC AATGACAAAC TGGAGAACGA GCTAGCCAAT
    AGGGAATCCT TGCATCGCCA GTGTGAGGAG AAAGCCCGAT ATCTGCAGGA GCTACTGGAG
    GTAGCCGAGC AGAAACTTCA GCAGACAATG CGCAAGGCCG AGACGCTGCC AGAGGTGGAG
    GCTGAGCTGG CCCAAAGAGT TGCGGCCCTC ACCAAGGCTG AAGAGCGACA TGGCAACACT
    GAGGAGCACC TCCGACAGCT GGAGGGACAG CTGGAGGAGA AGAACCAAGA GCTGGCGCGG
    GTGCGCCAGC GGGAGAGGAT GAATGAAGCC CACAACAAGC GCCTGTCAGA CACTGTGGAC
    CGGCTGCTTA TCGAGGCGAA GGAGCGCCTG CAGCTCCACC TCAGGGAGCG CATGGTGGCC
    CTGGAGGAGA AGAACACATT GATCCAAGAG CTGGAGACCT CCCAGCGGCA GATTGAAGAG
    CAGCACCATC ACAAGGGCCG CCTGTCTGAA GAGATTGAGA AACTGCGCCA AGAGGTGGAC
    CAACTGAAGG GTCGAGGAGG ACCATTTGTG GATGGCATCC ACTCCAGGGC GTACACGGGC
    AGTGCAATGG ATGTGCGGTT CTCCCTGAGC ACAACTGCCC ACACATCTCA AGGTCTGCGT
    CGTTGTTACT CAGCACGGCG GGAACCGCCT GCCAAGGACT GGGAGCCATC TCCACGGCCT
    GCGGTGCTGG TCCCGACAAC CACCCCTGCC TTTGACAGTG ACCGTGAGAT CTCTGATGTG
    GATGAGGATG AACCAGGGGG TCTGGTGGGC TCCATGGACG TTGTCTCCCC CAGCAGCCAC
    TCAGACGCCC AGACCCTGGC CATGATGCTG CAGGAGCAGC TGGATGCTAT CAACGAGGAG
    ATCAGGATGA TCCAGGAAGA GAAGGAGTCC ACAGAGCTCC GTGCTGAGGA GCTGGAAACT
    CGAGTGACTA GTGGCAGCAT GGAGGCCCTA GACCTGACCC AGCTGCACAA ACGCGGTTCC
    ATCCCCACCT CTCTGACCGC CCTGTCCCTG GCCAGCGCGT CCCCTCCACT TAGCGGCCGT
    GCCACACCTA AGCTCACCTC CCGCAGTGCT GCCCAGGACC TGGATCGGAT GGGGGTCATG
    ACCTTGCCCA GTGACTTAAG AAAGCATAGG AGGAAGCTGC TGTCACCAGT GTCTTGGGAA
    GAGAACCGAG AGGATAAAGC CACCATAAAA TGTGAGACTT CCCCTCCTTC CTCACCCAGG
    ACACTGCAGC TAGAGAAGCT TGGCCTCCCT GCCCTGAGCC AGGAAGAAGG CAAGAGTGCT
    TTGGAGGATC CAGGCAGCAA CCCCAGCAGC AGCAACAGCA GCCAGGACTC CTTGCACAAG
    GGTGCCAAGC GCAAGGGCAT CAAATCATCC ATCGGCCGCC TGTTTGGGAA GAAGGAGAAA
    GGCAGGCTGA TCCAGCCGAG CCGGGATGGA CCCACAGGCC ATGTTGTACT ACTAACAGAC
    TCTGAGCTCG GTCTGCAGGA GCCCATGGTG CCTGGCAAGC TGGGAACCCA GGCAGAAAAG
    GACCGGAGGC TGAAGAAGAA ACACCAGCTG CTTGAAGATG CCCGGAGAAA AGGAATGCCC
    TTTGCCCAGT GGGACGGCCC TACTGTGGTC TCCTGGCTAG AGCTCTGGGT GGGGATGCCT
    GCTTGGTATG TGGCAGCCTG CCGGGCCAAC GTCAAGAGTG GCGCCATCAT GTCAGCCCTA
    TCGGACACAG AGATCCAGCG AGAAATTGGC ATCAGCAATG CCCTGCACCG GCTCAAGCTC
    CGGCTGGCCA TCCAGGAGAT GGTGTCGCTG ACCAGCCCCT CTGCCCCGCC CACCTCCAGG
    ACTTCTTCTG GGAATGTCTG GGTCACCCAC GAAGAGATGG AAACTCTGGC AACGTCCACT
    AAAACAGACA GTGAGGAGGG CAGCTGGGCT CAGACCCTGG TCTATGGGGA TATGAACCAC
    GAGTGGATTG GGAACCAGTG GCTCCCCAGC CTGGGGCTCC CCCAGTACCG CAGCTACTTC
    ATGGAGTGCC TCGTGGACGC TCGCATGCTG GATCACCTCA CCAAGAAGGA CTTGCGGGTC
    CACCTGAAGA TGGTGGACAG CTCCCACCGA ACCAGTCTTC AGTATGGCAT CATGTGCCTG
    AAGAGGCTGA ATTATGACCG GAAGGAGCTG GAGAAGCGGC GGGAGGAGAG CCAGCATGAG
    ATCAAGGATG TGCTGGTCTG GACCAATGAC CAGGTAGTTC ATTGGGTCCA GTCTATTGGG
    CTCCGAGACT ACGCAGGAAA CCTCCATGAG AGTGGTGTGC ATGGCGCTTT GCTGGCCCTG
    GATGAGAACT TTGACCACAA CACACTGGCC CTGGTTCTCC AGATACCCAC ACAAAATACC
    CAGGCACGCC AAGTGTTGGA GAGAGAGTTC AACAACCTGT TGGCTTTGGG CACAGACCGG
    AAGCTGGATG ATGGGGAAGA CAAGGCGCTA CGCCGCGCGC CCTCCTGGAG GAAACGCTTC
    CGCCCGCGGG ACCCCCACGG CCGCGCCTCG CTCAGCGCCG CGGCCGACAC GCTCCCGGCA
    ACCTTCCGCG CGTCCACCGT GGGGCCCGTG CCGCCTCCAG CATCCCCGCG GAAGAAGATC
    ACGCCTGAAG GTCTGGACCG CTCACTCCAC CGGAGACCAG GGTGCAGAAC CAAGGGCTGG
    GGTGGCCCTT CCAAAGAAAG GGCGCTTCAG ACTTCCTTGC TTGGGTTATT GGGCCTTCTG
    GTTTCTGTCC GTCTCTTGGT TGCACTAGAT GGTCTACGTC TGCTTTTGGC ATCCCCCCTC
    CCGCCATTTT CTCTCCCCCA ACTCATCCGC ACCTTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025133602.1
    CDS169..3687
    Translation

    Target ORF information:

    RefSeq Version XM_025277817.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis PTPRF interacting protein alpha 4 (PPFIA4), transcript variant X16, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025277817.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGTGTCGGG AGCAGCTTCT AGAGAGGGAG GAAGAGATAT CAGAGCTGAA AGCAGAGCGG 
    AATAACACGA GGTTGCTTCT GGAACATCTG GAGTGCCTGG TGTCCCGCCA TGAGCGGTCA
    CTGAGGATGA CTGTGGTGAA GCGTCAGGCC CAGTCACCCT CAGGGGTCTC CAGTGAGGTG
    GAGGTGCTGA AGGCTCTCAA GTCACTGTTT GAGCACCACA AGGCCCTGGA TGAGAAGGTG
    CGAGAGCGGC TCCGGGTGGC CCTCGAGAGA GTGACCACTT TGGAGGAACA GCTGGCGTGT
    GCCCACCAGC AGGTGTCTTC CCTACAGCAG GGGTCAGGGG TTCGGGATGG AGTGGCCGAA
    GAGGAGGGGA CTGTGGAGCT GGGATCAAAA GGCCTGTGGA AGGAGGACAC AGGCCAGGTT
    GAGGAGCTGC AGGAGCTTCT GGAGAAGCAG AACTTTGAGT TGAGCCAGGC CCGGGAGCGA
    CTGGTCACCC TGACAGCAAC TGTGGCTGAA CTGGAGGAGG ACCTGGGCAC AGCCCGCCGG
    GACCTCATCA AGTCGGAGGA ACTGAGCAGC AAACACCAGC GGGACCTCCG GGAAGCTCTA
    GCCCAGAAAG AGGACATGGA GGAGCGGATC ACCACCCTGG AGAAGCGCTA CCTGGCTGCT
    CAGCGTGAGG CTACATCAAT ACATGACCTC AATGACAAAC TGGAGAACGA GCTAGCCAAT
    AGGGAATCCT TGCATCGCCA GTGTGAGGAG AAAGCCCGAT ATCTGCAGGA GCTACTGGAG
    GTAGCCGAGC AGAAACTTCA GCAGACAATG CGCAAGGCCG AGACGCTGCC AGAGGTGGAG
    GCTGAGCTGG CCCAAAGAGT TGCGGCCCTC ACCAAGGCTG AAGAGCGACA TGGCAACACT
    GAGGAGCACC TCCGACAGCT GGAGGGACAG CTGGAGGAGA AGAACCAAGA GCTGGCGCGG
    GTGCGCCAGC GGGAGAGGAT GAATGAAGCC CACAACAAGC GCCTGTCAGA CACTGTGGAC
    CGGCTGCTTA TCGAGGCGAA GGAGCGCCTG CAGCTCCACC TCAGGGAGCG CATGGTGGCC
    CTGGAGGAGA AGAACACATT GATCCAAGAG CTGGAGACCT CCCAGCGGCA GATTGAAGAG
    CAGCACCATC ACAAGGGCCG CCTGTCTGAA GAGATTGAGA AACTGCGCCA AGAGGTGGAC
    CAACTGAAGG GTCGAGGAGG ACCATTTGTG GATGGCATCC ACTCCAGGGC GTACACGGGC
    AGTGCAATGG ATGTGCGGTT CTCCCTGAGC ACAACTGCCC ACACATCTCA AGGTCTGCGT
    CGTTGTTACT CAGCACGGCG GGAACCGCCT GCCAAGGACT GGGAGCCATC TCCACGGCCT
    GCGGTGCTGG TCCCGACAAC CACCCCTGCC TTTGACAGTG ACCGTGAGAT CTCTGATGTG
    GATGAGGATG AACCAGGGGG TCTGGTGGGC TCCATGGACG TTGTCTCCCC CAGCAGCCAC
    TCAGACGCCC AGACCCTGGC CATGATGCTG CAGGAGCAGC TGGATGCTAT CAACGAGGAG
    ATCAGGATGA TCCAGGAAGA GAAGGAGTCC ACAGAGCTCC GTGCTGAGGA GCTGGAAACT
    CGAGTGACTA GTGGCAGCAT GGAGGCCCTA GACCTGACCC AGCTGCACAA ACGCGGTTCC
    ATCCCCACCT CTCTGACCGC CCTGTCCCTG GCCAGCGCGT CCCCTCCACT TAGCGGCCGT
    GCCACACCTA AGCTCACCTC CCGCAGTGCT GCCCAGGACC TGGATCGGAT GGGGGTCATG
    ACCTTGCCCA GTGACTTAAG AAAGCATAGG AGGAAGCTGC TGTCACCAGT GTCTTGGGAA
    GAGAACCGAG AGGATAAAGC CACCATAAAA TGTGAGACTT CCCCTCCTTC CTCACCCAGG
    ACACTGCAGC TAGAGAAGCT TGGCCTCCCT GCCCTGAGCC AGGAAGAAGG CAAGAGTGCT
    TTGGAGGATC CAGGCAGCAA CCCCAGCAGC AGCAACAGCA GCCAGGACTC CTTGCACAAG
    GGTGCCAAGC GCAAGGGCAT CAAATCATCC ATCGGCCGCC TGTTTGGGAA GAAGGAGAAA
    GGCAGGCTGA TCCAGCCGAG CCGGGATGGA CCCACAGGCC ATGTTGTACT ACTAACAGAC
    TCTGAGCTCG GTCTGCAGGA GCCCATGGTG CCTGGCAAGC TGGGAACCCA GGCAGAAAAG
    GACCGGAGGC TGAAGAAGAA ACACCAGCTG CTTGAAGATG CCCGGAGAAA AGGAATGCCC
    TTTGCCCAGT GGGACGGCCC TACTGTGGTC TCCTGGCTAG AGCTCTGGGT GGGGATGCCT
    GCTTGGTATG TGGCAGCCTG CCGGGCCAAC GTCAAGAGTG GCGCCATCAT GTCAGCCCTA
    TCGGACACAG AGATCCAGCG AGAAATTGGC ATCAGCAATG CCCTGCACCG GCTCAAGCTC
    CGGCTGGCCA TCCAGGAGAT GGTGTCGCTG ACCAGCCCCT CTGCCCCGCC CACCTCCAGG
    ACTTCTTCTG GGAATGTCTG GGTCACCCAC GAAGAGATGG AAACTCTGGC AACGTCCACT
    AAAACAGACA GTGAGGAGGG CAGCTGGGCT CAGACCCTGG TCTATGGGGA TATGAACCAC
    GAGTGGATTG GGAACCAGTG GCTCCCCAGC CTGGGGCTCC CCCAGTACCG CAGCTACTTC
    ATGGAGTGCC TCGTGGACGC TCGCATGCTG GATCACCTCA CCAAGAAGGA CTTGCGGGTC
    CACCTGAAGA TGGTGGACAG CTCCCACCGA ACCAGTCTTC AGTATGGCAT CATGTGCCTG
    AAGAGGCTGA ATTATGACCG GAAGGAGCTG GAGAAGCGGC GGGAGGAGAG CCAGCATGAG
    ATCAAGGATG TGCTGGTCTG GACCAATGAC CAGGTAGTTC ATTGGGTCCA GTCTATTGGG
    CTCCGAGACT ACGCAGGAAA CCTCCATGAG AGTGGTGTGC ATGGCGCTTT GCTGGCCCTG
    GATGAGAACT TTGACCACAA CACACTGGCC CTGGTTCTCC AGATACCCAC ACAAAATACC
    CAGGCACGCC AAGTGTTGGA GAGAGAGTTC AACAACCTGT TGGCTTTGGG CACAGACCGG
    AAGCTGGATG ATGGGGAAGA CAAGGCGCTA CGCCGCGCGC CCTCCTGGAG GAAACGCTTC
    CGCCCGCGGG ACCCCCACGG CCGCGCCTCG CTCAGCGCCG CGGCCGACAC GCTCCCGGCA
    ACCTTCCGCG CGTCCACCGT GGGGCCCGTG CCGCCTCCAG CATCCCCGCG GAAGAAGATC
    ACGCCTGAAG GTCTGGACCG CTCACTCCAC CGGAGACCAG GGTGCAGAAC CAAGGGCTGG
    GGTGGCCCTT CCAAAGAAAG GGCGCTTCAG ACTTCCTTGC TTGGGTTATT GGGCCTTCTG
    GTTTCTGTCC GTCTCTTGGT TGCACTAGAT GGTCTACGTC TGCTTTTGGC ATCCCCCCTC
    CCGCCATTTT CTCTCCCCCA ACTCATCCGC ACCTTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu41799
    Clone ID Related Accession (Same CDS sequence) XM_025277817.1 , XM_025277816.1
    Accession Version XM_025277816.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3519bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product liprin-alpha-4 isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_020228958.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGTGTCGGG AGCAGCTTCT AGAGAGGGAG GAAGAGATAT CAGAGCTGAA AGCAGAGCGG 
    AATAACACGA GGTTGCTTCT GGAACATCTG GAGTGCCTGG TGTCCCGCCA TGAGCGGTCA
    CTGAGGATGA CTGTGGTGAA GCGTCAGGCC CAGTCACCCT CAGGGGTCTC CAGTGAGGTG
    GAGGTGCTGA AGGCTCTCAA GTCACTGTTT GAGCACCACA AGGCCCTGGA TGAGAAGGTG
    CGAGAGCGGC TCCGGGTGGC CCTCGAGAGA GTGACCACTT TGGAGGAACA GCTGGCGTGT
    GCCCACCAGC AGGTGTCTTC CCTACAGCAG GGGTCAGGGG TTCGGGATGG AGTGGCCGAA
    GAGGAGGGGA CTGTGGAGCT GGGATCAAAA GGCCTGTGGA AGGAGGACAC AGGCCAGGTT
    GAGGAGCTGC AGGAGCTTCT GGAGAAGCAG AACTTTGAGT TGAGCCAGGC CCGGGAGCGA
    CTGGTCACCC TGACAGCAAC TGTGGCTGAA CTGGAGGAGG ACCTGGGCAC AGCCCGCCGG
    GACCTCATCA AGTCGGAGGA ACTGAGCAGC AAACACCAGC GGGACCTCCG GGAAGCTCTA
    GCCCAGAAAG AGGACATGGA GGAGCGGATC ACCACCCTGG AGAAGCGCTA CCTGGCTGCT
    CAGCGTGAGG CTACATCAAT ACATGACCTC AATGACAAAC TGGAGAACGA GCTAGCCAAT
    AGGGAATCCT TGCATCGCCA GTGTGAGGAG AAAGCCCGAT ATCTGCAGGA GCTACTGGAG
    GTAGCCGAGC AGAAACTTCA GCAGACAATG CGCAAGGCCG AGACGCTGCC AGAGGTGGAG
    GCTGAGCTGG CCCAAAGAGT TGCGGCCCTC ACCAAGGCTG AAGAGCGACA TGGCAACACT
    GAGGAGCACC TCCGACAGCT GGAGGGACAG CTGGAGGAGA AGAACCAAGA GCTGGCGCGG
    GTGCGCCAGC GGGAGAGGAT GAATGAAGCC CACAACAAGC GCCTGTCAGA CACTGTGGAC
    CGGCTGCTTA TCGAGGCGAA GGAGCGCCTG CAGCTCCACC TCAGGGAGCG CATGGTGGCC
    CTGGAGGAGA AGAACACATT GATCCAAGAG CTGGAGACCT CCCAGCGGCA GATTGAAGAG
    CAGCACCATC ACAAGGGCCG CCTGTCTGAA GAGATTGAGA AACTGCGCCA AGAGGTGGAC
    CAACTGAAGG GTCGAGGAGG ACCATTTGTG GATGGCATCC ACTCCAGGGC GTACACGGGC
    AGTGCAATGG ATGTGCGGTT CTCCCTGAGC ACAACTGCCC ACACATCTCA AGGTCTGCGT
    CGTTGTTACT CAGCACGGCG GGAACCGCCT GCCAAGGACT GGGAGCCATC TCCACGGCCT
    GCGGTGCTGG TCCCGACAAC CACCCCTGCC TTTGACAGTG ACCGTGAGAT CTCTGATGTG
    GATGAGGATG AACCAGGGGG TCTGGTGGGC TCCATGGACG TTGTCTCCCC CAGCAGCCAC
    TCAGACGCCC AGACCCTGGC CATGATGCTG CAGGAGCAGC TGGATGCTAT CAACGAGGAG
    ATCAGGATGA TCCAGGAAGA GAAGGAGTCC ACAGAGCTCC GTGCTGAGGA GCTGGAAACT
    CGAGTGACTA GTGGCAGCAT GGAGGCCCTA GACCTGACCC AGCTGCACAA ACGCGGTTCC
    ATCCCCACCT CTCTGACCGC CCTGTCCCTG GCCAGCGCGT CCCCTCCACT TAGCGGCCGT
    GCCACACCTA AGCTCACCTC CCGCAGTGCT GCCCAGGACC TGGATCGGAT GGGGGTCATG
    ACCTTGCCCA GTGACTTAAG AAAGCATAGG AGGAAGCTGC TGTCACCAGT GTCTTGGGAA
    GAGAACCGAG AGGATAAAGC CACCATAAAA TGTGAGACTT CCCCTCCTTC CTCACCCAGG
    ACACTGCAGC TAGAGAAGCT TGGCCTCCCT GCCCTGAGCC AGGAAGAAGG CAAGAGTGCT
    TTGGAGGATC CAGGCAGCAA CCCCAGCAGC AGCAACAGCA GCCAGGACTC CTTGCACAAG
    GGTGCCAAGC GCAAGGGCAT CAAATCATCC ATCGGCCGCC TGTTTGGGAA GAAGGAGAAA
    GGCAGGCTGA TCCAGCCGAG CCGGGATGGA CCCACAGGCC ATGTTGTACT ACTAACAGAC
    TCTGAGCTCG GTCTGCAGGA GCCCATGGTG CCTGGCAAGC TGGGAACCCA GGCAGAAAAG
    GACCGGAGGC TGAAGAAGAA ACACCAGCTG CTTGAAGATG CCCGGAGAAA AGGAATGCCC
    TTTGCCCAGT GGGACGGCCC TACTGTGGTC TCCTGGCTAG AGCTCTGGGT GGGGATGCCT
    GCTTGGTATG TGGCAGCCTG CCGGGCCAAC GTCAAGAGTG GCGCCATCAT GTCAGCCCTA
    TCGGACACAG AGATCCAGCG AGAAATTGGC ATCAGCAATG CCCTGCACCG GCTCAAGCTC
    CGGCTGGCCA TCCAGGAGAT GGTGTCGCTG ACCAGCCCCT CTGCCCCGCC CACCTCCAGG
    ACTTCTTCTG GGAATGTCTG GGTCACCCAC GAAGAGATGG AAACTCTGGC AACGTCCACT
    AAAACAGACA GTGAGGAGGG CAGCTGGGCT CAGACCCTGG TCTATGGGGA TATGAACCAC
    GAGTGGATTG GGAACCAGTG GCTCCCCAGC CTGGGGCTCC CCCAGTACCG CAGCTACTTC
    ATGGAGTGCC TCGTGGACGC TCGCATGCTG GATCACCTCA CCAAGAAGGA CTTGCGGGTC
    CACCTGAAGA TGGTGGACAG CTCCCACCGA ACCAGTCTTC AGTATGGCAT CATGTGCCTG
    AAGAGGCTGA ATTATGACCG GAAGGAGCTG GAGAAGCGGC GGGAGGAGAG CCAGCATGAG
    ATCAAGGATG TGCTGGTCTG GACCAATGAC CAGGTAGTTC ATTGGGTCCA GTCTATTGGG
    CTCCGAGACT ACGCAGGAAA CCTCCATGAG AGTGGTGTGC ATGGCGCTTT GCTGGCCCTG
    GATGAGAACT TTGACCACAA CACACTGGCC CTGGTTCTCC AGATACCCAC ACAAAATACC
    CAGGCACGCC AAGTGTTGGA GAGAGAGTTC AACAACCTGT TGGCTTTGGG CACAGACCGG
    AAGCTGGATG ATGGGGAAGA CAAGGCGCTA CGCCGCGCGC CCTCCTGGAG GAAACGCTTC
    CGCCCGCGGG ACCCCCACGG CCGCGCCTCG CTCAGCGCCG CGGCCGACAC GCTCCCGGCA
    ACCTTCCGCG CGTCCACCGT GGGGCCCGTG CCGCCTCCAG CATCCCCGCG GAAGAAGATC
    ACGCCTGAAG GTCTGGACCG CTCACTCCAC CGGAGACCAG GGTGCAGAAC CAAGGGCTGG
    GGTGGCCCTT CCAAAGAAAG GGCGCTTCAG ACTTCCTTGC TTGGGTTATT GGGCCTTCTG
    GTTTCTGTCC GTCTCTTGGT TGCACTAGAT GGTCTACGTC TGCTTTTGGC ATCCCCCCTC
    CCGCCATTTT CTCTCCCCCA ACTCATCCGC ACCTTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025133601.1
    CDS300..3818
    Translation

    Target ORF information:

    RefSeq Version XM_025277816.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis PTPRF interacting protein alpha 4 (PPFIA4), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025277816.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGTGTCGGG AGCAGCTTCT AGAGAGGGAG GAAGAGATAT CAGAGCTGAA AGCAGAGCGG 
    AATAACACGA GGTTGCTTCT GGAACATCTG GAGTGCCTGG TGTCCCGCCA TGAGCGGTCA
    CTGAGGATGA CTGTGGTGAA GCGTCAGGCC CAGTCACCCT CAGGGGTCTC CAGTGAGGTG
    GAGGTGCTGA AGGCTCTCAA GTCACTGTTT GAGCACCACA AGGCCCTGGA TGAGAAGGTG
    CGAGAGCGGC TCCGGGTGGC CCTCGAGAGA GTGACCACTT TGGAGGAACA GCTGGCGTGT
    GCCCACCAGC AGGTGTCTTC CCTACAGCAG GGGTCAGGGG TTCGGGATGG AGTGGCCGAA
    GAGGAGGGGA CTGTGGAGCT GGGATCAAAA GGCCTGTGGA AGGAGGACAC AGGCCAGGTT
    GAGGAGCTGC AGGAGCTTCT GGAGAAGCAG AACTTTGAGT TGAGCCAGGC CCGGGAGCGA
    CTGGTCACCC TGACAGCAAC TGTGGCTGAA CTGGAGGAGG ACCTGGGCAC AGCCCGCCGG
    GACCTCATCA AGTCGGAGGA ACTGAGCAGC AAACACCAGC GGGACCTCCG GGAAGCTCTA
    GCCCAGAAAG AGGACATGGA GGAGCGGATC ACCACCCTGG AGAAGCGCTA CCTGGCTGCT
    CAGCGTGAGG CTACATCAAT ACATGACCTC AATGACAAAC TGGAGAACGA GCTAGCCAAT
    AGGGAATCCT TGCATCGCCA GTGTGAGGAG AAAGCCCGAT ATCTGCAGGA GCTACTGGAG
    GTAGCCGAGC AGAAACTTCA GCAGACAATG CGCAAGGCCG AGACGCTGCC AGAGGTGGAG
    GCTGAGCTGG CCCAAAGAGT TGCGGCCCTC ACCAAGGCTG AAGAGCGACA TGGCAACACT
    GAGGAGCACC TCCGACAGCT GGAGGGACAG CTGGAGGAGA AGAACCAAGA GCTGGCGCGG
    GTGCGCCAGC GGGAGAGGAT GAATGAAGCC CACAACAAGC GCCTGTCAGA CACTGTGGAC
    CGGCTGCTTA TCGAGGCGAA GGAGCGCCTG CAGCTCCACC TCAGGGAGCG CATGGTGGCC
    CTGGAGGAGA AGAACACATT GATCCAAGAG CTGGAGACCT CCCAGCGGCA GATTGAAGAG
    CAGCACCATC ACAAGGGCCG CCTGTCTGAA GAGATTGAGA AACTGCGCCA AGAGGTGGAC
    CAACTGAAGG GTCGAGGAGG ACCATTTGTG GATGGCATCC ACTCCAGGGC GTACACGGGC
    AGTGCAATGG ATGTGCGGTT CTCCCTGAGC ACAACTGCCC ACACATCTCA AGGTCTGCGT
    CGTTGTTACT CAGCACGGCG GGAACCGCCT GCCAAGGACT GGGAGCCATC TCCACGGCCT
    GCGGTGCTGG TCCCGACAAC CACCCCTGCC TTTGACAGTG ACCGTGAGAT CTCTGATGTG
    GATGAGGATG AACCAGGGGG TCTGGTGGGC TCCATGGACG TTGTCTCCCC CAGCAGCCAC
    TCAGACGCCC AGACCCTGGC CATGATGCTG CAGGAGCAGC TGGATGCTAT CAACGAGGAG
    ATCAGGATGA TCCAGGAAGA GAAGGAGTCC ACAGAGCTCC GTGCTGAGGA GCTGGAAACT
    CGAGTGACTA GTGGCAGCAT GGAGGCCCTA GACCTGACCC AGCTGCACAA ACGCGGTTCC
    ATCCCCACCT CTCTGACCGC CCTGTCCCTG GCCAGCGCGT CCCCTCCACT TAGCGGCCGT
    GCCACACCTA AGCTCACCTC CCGCAGTGCT GCCCAGGACC TGGATCGGAT GGGGGTCATG
    ACCTTGCCCA GTGACTTAAG AAAGCATAGG AGGAAGCTGC TGTCACCAGT GTCTTGGGAA
    GAGAACCGAG AGGATAAAGC CACCATAAAA TGTGAGACTT CCCCTCCTTC CTCACCCAGG
    ACACTGCAGC TAGAGAAGCT TGGCCTCCCT GCCCTGAGCC AGGAAGAAGG CAAGAGTGCT
    TTGGAGGATC CAGGCAGCAA CCCCAGCAGC AGCAACAGCA GCCAGGACTC CTTGCACAAG
    GGTGCCAAGC GCAAGGGCAT CAAATCATCC ATCGGCCGCC TGTTTGGGAA GAAGGAGAAA
    GGCAGGCTGA TCCAGCCGAG CCGGGATGGA CCCACAGGCC ATGTTGTACT ACTAACAGAC
    TCTGAGCTCG GTCTGCAGGA GCCCATGGTG CCTGGCAAGC TGGGAACCCA GGCAGAAAAG
    GACCGGAGGC TGAAGAAGAA ACACCAGCTG CTTGAAGATG CCCGGAGAAA AGGAATGCCC
    TTTGCCCAGT GGGACGGCCC TACTGTGGTC TCCTGGCTAG AGCTCTGGGT GGGGATGCCT
    GCTTGGTATG TGGCAGCCTG CCGGGCCAAC GTCAAGAGTG GCGCCATCAT GTCAGCCCTA
    TCGGACACAG AGATCCAGCG AGAAATTGGC ATCAGCAATG CCCTGCACCG GCTCAAGCTC
    CGGCTGGCCA TCCAGGAGAT GGTGTCGCTG ACCAGCCCCT CTGCCCCGCC CACCTCCAGG
    ACTTCTTCTG GGAATGTCTG GGTCACCCAC GAAGAGATGG AAACTCTGGC AACGTCCACT
    AAAACAGACA GTGAGGAGGG CAGCTGGGCT CAGACCCTGG TCTATGGGGA TATGAACCAC
    GAGTGGATTG GGAACCAGTG GCTCCCCAGC CTGGGGCTCC CCCAGTACCG CAGCTACTTC
    ATGGAGTGCC TCGTGGACGC TCGCATGCTG GATCACCTCA CCAAGAAGGA CTTGCGGGTC
    CACCTGAAGA TGGTGGACAG CTCCCACCGA ACCAGTCTTC AGTATGGCAT CATGTGCCTG
    AAGAGGCTGA ATTATGACCG GAAGGAGCTG GAGAAGCGGC GGGAGGAGAG CCAGCATGAG
    ATCAAGGATG TGCTGGTCTG GACCAATGAC CAGGTAGTTC ATTGGGTCCA GTCTATTGGG
    CTCCGAGACT ACGCAGGAAA CCTCCATGAG AGTGGTGTGC ATGGCGCTTT GCTGGCCCTG
    GATGAGAACT TTGACCACAA CACACTGGCC CTGGTTCTCC AGATACCCAC ACAAAATACC
    CAGGCACGCC AAGTGTTGGA GAGAGAGTTC AACAACCTGT TGGCTTTGGG CACAGACCGG
    AAGCTGGATG ATGGGGAAGA CAAGGCGCTA CGCCGCGCGC CCTCCTGGAG GAAACGCTTC
    CGCCCGCGGG ACCCCCACGG CCGCGCCTCG CTCAGCGCCG CGGCCGACAC GCTCCCGGCA
    ACCTTCCGCG CGTCCACCGT GGGGCCCGTG CCGCCTCCAG CATCCCCGCG GAAGAAGATC
    ACGCCTGAAG GTCTGGACCG CTCACTCCAC CGGAGACCAG GGTGCAGAAC CAAGGGCTGG
    GGTGGCCCTT CCAAAGAAAG GGCGCTTCAG ACTTCCTTGC TTGGGTTATT GGGCCTTCTG
    GTTTCTGTCC GTCTCTTGGT TGCACTAGAT GGTCTACGTC TGCTTTTGGC ATCCCCCCTC
    CCGCCATTTT CTCTCCCCCA ACTCATCCGC ACCTTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu26664
    Clone ID Related Accession (Same CDS sequence) XM_006072413.1
    Accession Version XM_006072413.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3609bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2014-03-11
    Organism Bubalus bubalis(water buffalo)
    Product liprin-alpha-4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785489.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGTGTGAAG TGATGCCCAC AATCAACGAG GGAGACCCCC TGGGGCCTCC CCATGGAGCC 
    GATGCTGACG CCAACTTTGA GCAGCTGATG GTGAACATGC TGGATGAGCG GGAGAAGTTG
    CTAGAGTCCC TTCGGGAGAG TCAGGAGACT TTGCTGGCGA CACAGAGCCG GCTCCAGGAT
    GCCCTGCATG AGCGAGACCA GCTCCAGCGC CACCTTAACT CTGCCCTCCC CCAGGAGTTT
    ACCACCTTAA CCCGGGAGCT GAGTATGTGT CGGGAGCAGC TTCTAGAGAG GGAGGAAGAG
    ATATCAGAGC TGAAAGCAGA GCGGAATAAC ACGAGGTTGC TTCTGGAACA TCTGGAGTGC
    CTGGTGTCCC GCCATGAGCG GTCACTGAGG ATGACTGTGG TGAAGCGTCA GGCCCAGTCA
    CCCTCAGGGG TCTCCAGTGA GGTGGAGGTG CTGAAGGCTC TCAAGTCACT GTTTGAGCAC
    CACAAGGCCC TGGATGAGAA GGTACCTAGG CTAGCCGACA GCCTGCATCC ACCCCCGATG
    CAACCATGGG TACAATACAA TACAGTCCAG TCCACCCCCA GTCTCTTCCC TGGAGCCTCC
    ATGCATCCCT TCTCTGAGAT TTGTGTATCC TCTAGCTCCC TGTCTCTGGA GGAGGACACA
    GGCCAGGTTG AGGAGCTGCA GGAGCTTCTG GAGAAGCAGA ACTTTGAGTT GAGCCAGGCC
    CGGGAGCGAC TGGTCACCCT GACAGCAACT GTGGCTGAAC TGGAGGAGGA CCTGGGCACA
    GCCCGCCGGG ACCTCATCAA GTCGGAGGAA CTGAGCAGCA AACACCAGCG GGACCTCCGG
    GAAGCTCTAG CCCAGAAAGA GGACATGGAG GAGCGGATCA CCACCCTGGA GAAGCGCTAC
    CTGGCTGCTC AGCGTGAGGC TACATCAATA CATGACCTCA ATGACAAACT GGAGAACGAG
    CTAGCCAATA GGGAATCCTT GCATCGCCAG TGTGAGGAGA AAGCCCGATA TCTGCAGGAG
    CTACTGGAGG TAGCCGAGCA GAAACTTCAG CAGACAATGC GCAAGGCCGA GACGCTGCCA
    GAGGTGGAGG CTGAGCTGGC CCAAAGAGTT GCGGCCCTCA CCAAGGCTGA AGAGCGACAT
    GGCAACACTG AGGAGCACCT CCGACAGCTG GAGGGACAGC TGGAGGAGAA GAACCAAGAG
    CTGGCGCGGG TGCGCCAGCG GGAGAGGATG AATGAAGCCC ACAACAAGCG CCTGTCAGAC
    ACTGTGGACC GGCTGCTTAT CGAGGCGAAG GAGCGCCTGC AGCTCCACCT CAGGGAGCGC
    ATGGTGGCCC TGGAGGAGAA GAACACATTG ATCCAAGAGC TGGAGACCTC CCAGCGGCAG
    ATTGAAGAGC AGCACCATCA CAAGGGCCGC CTGTCTGAAG AGATTGAGAA ACTGCGCCAA
    GAGGTGGACC AACTGAAGGG TCGAGGAGGA CCATTTGTGG ATGGCATCCA CTCCAGGGCG
    TACACGGGCA GTGCAATGGA TGTGCGGTTC TCCCTGAGCA CAACTGCCCA CACATCTCAA
    GGTCTGCGTC GTTGTTACTC AGCACGGCGG GAACCGCCTG CCAAGGACTG GGAGCCATCT
    CCACGGCCTG CGGTGCTGGT CCCGACAACC ACCCCTGCCT TTGACAGTGA CCGTGAGATC
    TCTGATGTGG ATGAGGATGA ACCAGGGGGT CTGGTGGGCT CCATGGACGT TGTCTCCCCC
    AGCAGCCACT CAGACGCCCA GACCCTGGCC ATGATGCTGC AGGAGCAGCT GGATGCTATC
    AACGAGGAGA TCAGGATGAT CCAGGAAGAG AAGGAGTCCA CAGAGCTCCG TGCTGAGGAG
    CTGGAAACTC GAGTGACTAG TGGCAGCATG GAGGCCCTAG ACCTGACCCA GCTGCACAAA
    CGCGGTTCCA TCCCCACCTC TCTGACCGCC CTGTCCCTGG CCAGCGCGTC CCCTCCACTT
    AGCGGCCGTG CCACACCTAA GCTTACCTCC CGCAGTGCTG CCCAGGACCT GGATCGGATG
    GGGGTCATGA CCTTGCCCAG TGACTTAAGA AAGCATAGGA GGAAGCTGCT GTCACCAGTG
    TCTTGGGAAG AGAACCGAGA GGATAAAGCC ACCATAAAAT GTGAGACTTC CCCTCCTTCC
    TCACCCAGGA CACTGCAGCT AGAGAAGCTT GGCCTCCCTG CCCTGAGCCA GGAAGAAGGC
    AAGAGTGCTT TGGAGGATCC AGGCAGCAAC CCCAGCAGCA GCAACAGCAG CCAGGACTCC
    TTGCACAAGG GTGCCAAGCG CAAGGGCATC AAATCATCCA TCGGCCGCCT GTTTGGGAAG
    AAGGAGAAAG GCAGGCTGAT CCAGCCGAGC CGGGATGGAC CCACAGGCCA TGTTGTACTA
    CTAACAGACT CTGAGCTCGG TCTGCAGGAG CCCATGGTGC CTGGCAAGCT GGGAACCCAG
    GCAGAAAAGG ACCGGAGGCT GAAGAAGAAA CACCAGCTGC TTGAAGATGC CCGGAGAAAA
    GGAATGCCCT TTGCCCAGTG GGACGGCCCT ACTGTGGTCT CCTGGCTAGA GCTCTGGGTG
    GGGATGCCTG CTTGGTATGT GGCAGCCTGC CGGGCCAACG TCAAGAGTGG CGCCATCATG
    TCAGCCCTAT CGGACACAGA GATCCAGCGA GAAATTGGCA TCAGCAATGC CCTGCACCGG
    CTCAAGCTCC GGCTGGCCAT CCAGGAGATG GTGTCGCTGA CCAGCCCCTC TGCCCCGCCC
    ACCTCCAGGA CTTCTTCTGG GAATGTCTGG GTCACCCACG AAGAGATGGA AACTCTGGCA
    ACGTCCACTA AAACAGACAG TGAGGAGGGC AGCTGGGCTC AGACCCTGGT CTATGGGGAT
    ATGAACCACG AGTGGATTGG GAACCAGTGG CTCCCCAGCC TGGGGCTCCC CCAGTACCGC
    AGCTACTTCA TGGAGTGCCT CGTGGACGCT CGCATGCTGG ATCACCTCAC CAAGAAGGAC
    TTGCGGGTCC ACCTGAAGAT GGTGGACAGC TCCCACCGAA CCAGTCTTCA GTATGGCATC
    ATGTGCCTGA AGAGGCTGAA TTATGACCGG AAGGAGCTGG AGAAGCGGCG GGAGGAGAGC
    CAGCATGAGA TCAAGGATGT GCTGGTCTGG ACCAATGACC AGGTAGTTCA TTGGGTCCAG
    TCTATTGGGC TCCGAGACTA CGCAGGAAAC CTCCATGAGA GTGGTGTGCA TGGCGCTTTG
    CTGGCCCTGG ATGAGAACTT TGACCACAAC ACACTGGCCC TGGTTCTCCA GATACCCACA
    CAAAATACCC AGGCACGCCA AGTGTTGGAG AGAGAGTTCA ACAACCTGTT GGCTTTGGGC
    ACAGACCGGA AGCTGGATGA TGGGGAAGAC AAGGCGCTAC GCCGCGCGCC CTCCTGGAGG
    AAACGCTTCC GCCCGCGGGA CCCCCACGGC CGCGCCTCGC TCAGCGCCGC GGCCGACACG
    CTCCCGGCAA CCTTCCGCGC GTCCACCGTG GGGCCCGTGC CGCCTTCAGC ATCCCCGCGG
    AAGAAGATCA CGCCTGAAGC TCGCTCCCAC TATCTCTACG GACACATGCT CTCCGCCTTC
    CGGGACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006072475.1
    CDS241..3849
    Translation

    Target ORF information:

    RefSeq Version XM_006072413.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis protein tyrosine phosphatase, receptor type, f polypeptide (PTPRF), interacting protein (liprin), alpha 4 (PPFIA4), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006072413.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGTGTGAAG TGATGCCCAC AATCAACGAG GGAGACCCCC TGGGGCCTCC CCATGGAGCC 
    GATGCTGACG CCAACTTTGA GCAGCTGATG GTGAACATGC TGGATGAGCG GGAGAAGTTG
    CTAGAGTCCC TTCGGGAGAG TCAGGAGACT TTGCTGGCGA CACAGAGCCG GCTCCAGGAT
    GCCCTGCATG AGCGAGACCA GCTCCAGCGC CACCTTAACT CTGCCCTCCC CCAGGAGTTT
    ACCACCTTAA CCCGGGAGCT GAGTATGTGT CGGGAGCAGC TTCTAGAGAG GGAGGAAGAG
    ATATCAGAGC TGAAAGCAGA GCGGAATAAC ACGAGGTTGC TTCTGGAACA TCTGGAGTGC
    CTGGTGTCCC GCCATGAGCG GTCACTGAGG ATGACTGTGG TGAAGCGTCA GGCCCAGTCA
    CCCTCAGGGG TCTCCAGTGA GGTGGAGGTG CTGAAGGCTC TCAAGTCACT GTTTGAGCAC
    CACAAGGCCC TGGATGAGAA GGTACCTAGG CTAGCCGACA GCCTGCATCC ACCCCCGATG
    CAACCATGGG TACAATACAA TACAGTCCAG TCCACCCCCA GTCTCTTCCC TGGAGCCTCC
    ATGCATCCCT TCTCTGAGAT TTGTGTATCC TCTAGCTCCC TGTCTCTGGA GGAGGACACA
    GGCCAGGTTG AGGAGCTGCA GGAGCTTCTG GAGAAGCAGA ACTTTGAGTT GAGCCAGGCC
    CGGGAGCGAC TGGTCACCCT GACAGCAACT GTGGCTGAAC TGGAGGAGGA CCTGGGCACA
    GCCCGCCGGG ACCTCATCAA GTCGGAGGAA CTGAGCAGCA AACACCAGCG GGACCTCCGG
    GAAGCTCTAG CCCAGAAAGA GGACATGGAG GAGCGGATCA CCACCCTGGA GAAGCGCTAC
    CTGGCTGCTC AGCGTGAGGC TACATCAATA CATGACCTCA ATGACAAACT GGAGAACGAG
    CTAGCCAATA GGGAATCCTT GCATCGCCAG TGTGAGGAGA AAGCCCGATA TCTGCAGGAG
    CTACTGGAGG TAGCCGAGCA GAAACTTCAG CAGACAATGC GCAAGGCCGA GACGCTGCCA
    GAGGTGGAGG CTGAGCTGGC CCAAAGAGTT GCGGCCCTCA CCAAGGCTGA AGAGCGACAT
    GGCAACACTG AGGAGCACCT CCGACAGCTG GAGGGACAGC TGGAGGAGAA GAACCAAGAG
    CTGGCGCGGG TGCGCCAGCG GGAGAGGATG AATGAAGCCC ACAACAAGCG CCTGTCAGAC
    ACTGTGGACC GGCTGCTTAT CGAGGCGAAG GAGCGCCTGC AGCTCCACCT CAGGGAGCGC
    ATGGTGGCCC TGGAGGAGAA GAACACATTG ATCCAAGAGC TGGAGACCTC CCAGCGGCAG
    ATTGAAGAGC AGCACCATCA CAAGGGCCGC CTGTCTGAAG AGATTGAGAA ACTGCGCCAA
    GAGGTGGACC AACTGAAGGG TCGAGGAGGA CCATTTGTGG ATGGCATCCA CTCCAGGGCG
    TACACGGGCA GTGCAATGGA TGTGCGGTTC TCCCTGAGCA CAACTGCCCA CACATCTCAA
    GGTCTGCGTC GTTGTTACTC AGCACGGCGG GAACCGCCTG CCAAGGACTG GGAGCCATCT
    CCACGGCCTG CGGTGCTGGT CCCGACAACC ACCCCTGCCT TTGACAGTGA CCGTGAGATC
    TCTGATGTGG ATGAGGATGA ACCAGGGGGT CTGGTGGGCT CCATGGACGT TGTCTCCCCC
    AGCAGCCACT CAGACGCCCA GACCCTGGCC ATGATGCTGC AGGAGCAGCT GGATGCTATC
    AACGAGGAGA TCAGGATGAT CCAGGAAGAG AAGGAGTCCA CAGAGCTCCG TGCTGAGGAG
    CTGGAAACTC GAGTGACTAG TGGCAGCATG GAGGCCCTAG ACCTGACCCA GCTGCACAAA
    CGCGGTTCCA TCCCCACCTC TCTGACCGCC CTGTCCCTGG CCAGCGCGTC CCCTCCACTT
    AGCGGCCGTG CCACACCTAA GCTTACCTCC CGCAGTGCTG CCCAGGACCT GGATCGGATG
    GGGGTCATGA CCTTGCCCAG TGACTTAAGA AAGCATAGGA GGAAGCTGCT GTCACCAGTG
    TCTTGGGAAG AGAACCGAGA GGATAAAGCC ACCATAAAAT GTGAGACTTC CCCTCCTTCC
    TCACCCAGGA CACTGCAGCT AGAGAAGCTT GGCCTCCCTG CCCTGAGCCA GGAAGAAGGC
    AAGAGTGCTT TGGAGGATCC AGGCAGCAAC CCCAGCAGCA GCAACAGCAG CCAGGACTCC
    TTGCACAAGG GTGCCAAGCG CAAGGGCATC AAATCATCCA TCGGCCGCCT GTTTGGGAAG
    AAGGAGAAAG GCAGGCTGAT CCAGCCGAGC CGGGATGGAC CCACAGGCCA TGTTGTACTA
    CTAACAGACT CTGAGCTCGG TCTGCAGGAG CCCATGGTGC CTGGCAAGCT GGGAACCCAG
    GCAGAAAAGG ACCGGAGGCT GAAGAAGAAA CACCAGCTGC TTGAAGATGC CCGGAGAAAA
    GGAATGCCCT TTGCCCAGTG GGACGGCCCT ACTGTGGTCT CCTGGCTAGA GCTCTGGGTG
    GGGATGCCTG CTTGGTATGT GGCAGCCTGC CGGGCCAACG TCAAGAGTGG CGCCATCATG
    TCAGCCCTAT CGGACACAGA GATCCAGCGA GAAATTGGCA TCAGCAATGC CCTGCACCGG
    CTCAAGCTCC GGCTGGCCAT CCAGGAGATG GTGTCGCTGA CCAGCCCCTC TGCCCCGCCC
    ACCTCCAGGA CTTCTTCTGG GAATGTCTGG GTCACCCACG AAGAGATGGA AACTCTGGCA
    ACGTCCACTA AAACAGACAG TGAGGAGGGC AGCTGGGCTC AGACCCTGGT CTATGGGGAT
    ATGAACCACG AGTGGATTGG GAACCAGTGG CTCCCCAGCC TGGGGCTCCC CCAGTACCGC
    AGCTACTTCA TGGAGTGCCT CGTGGACGCT CGCATGCTGG ATCACCTCAC CAAGAAGGAC
    TTGCGGGTCC ACCTGAAGAT GGTGGACAGC TCCCACCGAA CCAGTCTTCA GTATGGCATC
    ATGTGCCTGA AGAGGCTGAA TTATGACCGG AAGGAGCTGG AGAAGCGGCG GGAGGAGAGC
    CAGCATGAGA TCAAGGATGT GCTGGTCTGG ACCAATGACC AGGTAGTTCA TTGGGTCCAG
    TCTATTGGGC TCCGAGACTA CGCAGGAAAC CTCCATGAGA GTGGTGTGCA TGGCGCTTTG
    CTGGCCCTGG ATGAGAACTT TGACCACAAC ACACTGGCCC TGGTTCTCCA GATACCCACA
    CAAAATACCC AGGCACGCCA AGTGTTGGAG AGAGAGTTCA ACAACCTGTT GGCTTTGGGC
    ACAGACCGGA AGCTGGATGA TGGGGAAGAC AAGGCGCTAC GCCGCGCGCC CTCCTGGAGG
    AAACGCTTCC GCCCGCGGGA CCCCCACGGC CGCGCCTCGC TCAGCGCCGC GGCCGACACG
    CTCCCGGCAA CCTTCCGCGC GTCCACCGTG GGGCCCGTGC CGCCTTCAGC ATCCCCGCGG
    AAGAAGATCA CGCCTGAAGC TCGCTCCCAC TATCTCTACG GACACATGCT CTCCGCCTTC
    CGGGACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.