HMGCR cDNA ORF clone, Bubalus bubalis(water buffalo)

The following HMGCR gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HMGCR cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBu28003 XM_006074029.2
Latest version!
Bubalus bubalis 3-hydroxy-3-methylglutaryl-CoA reductase (HMGCR), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OBu28003 XM_006074030.2
Latest version!
Bubalus bubalis 3-hydroxy-3-methylglutaryl-CoA reductase (HMGCR), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OBu28003 XM_006074032.1
Latest version!
Bubalus bubalis 3-hydroxy-3-methylglutaryl-CoA reductase (HMGCR), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OBu28003 XM_025296388.1
Latest version!
Bubalus bubalis 3-hydroxy-3-methylglutaryl-CoA reductase (HMGCR), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OBu28003 XM_006074031.2
Latest version!
Bubalus bubalis 3-hydroxy-3-methylglutaryl-CoA reductase (HMGCR), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OBu45465 XM_025296389.1
Latest version!
Bubalus bubalis 3-hydroxy-3-methylglutaryl-CoA reductase (HMGCR), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
View Old Accession Versions >>
OBu28003 XM_006074029.1 Bubalus bubalis 3-hydroxy-3-methylglutaryl-CoA reductase (HMGCR), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OBu28003 XM_006074030.1 Bubalus bubalis 3-hydroxy-3-methylglutaryl-CoA reductase (HMGCR), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OBu28003 XM_006074031.1 Bubalus bubalis 3-hydroxy-3-methylglutaryl-CoA reductase (HMGCR), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBu28003
    Clone ID Related Accession (Same CDS sequence) XM_006074030.1 , XM_006074029.2 , XM_006074029.1 , XM_006074032.1 , XM_006074031.2 , XM_006074030.2 , XM_025296388.1 , XM_006074031.1
    Accession Version XM_006074030.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2667bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2014-03-11
    Organism Bubalus bubalis(water buffalo)
    Product 3-hydroxy-3-methylglutaryl-coenzyme A reductase isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785570.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGTTGTCAA GACTTTTCCG AATGCACGGC CTCTTTGTGG CCTCCCATCC CTGGGAAGTC 
    ATAGTGGGGA CAGTGACGCT GACCATCTGC ATGATGTCCA TGAACATGTT TACTGGCAAC
    AATAAGATCT GTGGTTGGAA TTATGAATGT CCGAAGCTTG AAGAGGATGT TTTGAGCAGC
    GACATCATAA TTCTGACAAT AACACGATGC ATAGCAATAT TGTATATTTA CTTCCAGTTC
    CAGAATTTAC GCCAACTTGG ATCAAAATAT ATTTTGGGTA TTGCTGGGCT CTTCACAATT
    TTCTCCAGTT TCGTATTCAG TACAGTTGTC ATTCATGTCT TAGATAAAGA ATTGACAGGT
    TTGAATGAAG CTTTGCCCTT TTTCCTGCTG CTGGTCGACC TTTCTAGAGC AAGTGCGCTA
    GCCAAGTTCG CCCTCAGTTC TAACTCACAG GATGAAGTGA GGGAGAACAT TGCTCGTGGG
    ATGGCGATTT TAGGTCCCAC CTTCACCCTG GATGCTCTTG TAGAATGTCT CGTGATTGGA
    GTTGGTACCA TGTCAGGGGT GCGTCAGCTC GAAATCATGT GCTGCTTTGG CTGCATGTCG
    GTTCTCGCCA ACTACTTCGT GTTCATGACT TTCTTCCCAG CTTGTGTGTC CTTGGTTCTG
    GAGCTTTCTC GGGAAAGCCG CGAGGGCCGT CCAATCTGGC AGCTCAGCCA TTTTGCCCGA
    GTCTTAGAAG AAGAAGAAAA TAAGCCAAAT CCTGTTACTC AGAGGGTTAA GATGATTATG
    TCTCTAGGCT TGGTTCTTGT TCACGCTCAC AGTCGCTGGA TAGCTGATCC TTCTCCTCAA
    AACAGCACAG CAGACAATTC TAAGGTCTCT TTAGGATTGG ATGAAAATGT GTCCAAGAGA
    ATTGAACCGA GTGTTTCCCT CTGGCAGTTT TATCTCTCTA AAATGATCAG CATGGATATT
    GAACAAGTTA TTACCCTAAG TTTAGCTCTC CTTCTGGCTG TCAAGTATAT CTTCTTTGAA
    CAAGCAGAGA CAGAATCGAC ACTCTCGTTA AAAAATCCTA TCACATCTCC TGTAGTGACC
    CAAAAGAAAG TCACAGACGA TTGTTGTAGA CGTGACCCTG TACTGGTCAG AAATGACCAG
    AAATTCCATG CAATGGAGGA GGAGGCCAGG AAAAACAGAG AAAGAAAAGT TGAGGTTATA
    AAACCCTTAC TGGCAGAAAA TGACACCTCA AACAGAGCTA CATTTGTGGT GGGGAACTCC
    TCCTTACTCA GTACTTCACT GGAACTAGAG ACACAGGAAC CTGAAATTGA ACTTCCCGTG
    GAGCCTCGGC CTAATGAAGA GTGTCTGCAG ATTCTTGAGA ATGCAGAGAA AGGTGCAAAA
    TTCCTTAGTG ATGCTGAGAT CATCCAGTTG GTCAATGCAA AGCATATCCC AGCTTACAAA
    TTGGAAACTC TGATGGAAAC CCATGAGCGA GGTGTATCTA TTCGCCGACA GCTACTTTCC
    AAAAAACTTC CAGAGCCTTC TTCTCTCCAG TATCTACCTT ACAGGGACTA TAATTACTCC
    TTGGTGATGG GAGCTTGCTG TGAGAATGTT ATTGGATATA TGCCCATCCC TGTTGGAGTG
    GCAGGACCTC TGTGCTTGGA TGGAAAAGAA TTTCAGGTTC CAATGGCAAC AACAGAAGGC
    TGTCTCGTGG CCAGCACTAA TAGAGGCTGC AGAGCAATAG GCCTTGGTGG AGGTGCCAGC
    AGCCGGGTCC TTGCAGACGG GATGACTCGT GGCCCAGTGG TGCGTTTTCC CCGTGCTTGT
    GACTCTGCAG AAGTGAAGGC CTGGCTTGAG ACGCCTGAAG GGTTCACAGT GATAAAGGAA
    GCATTCGACA GCACCAGCAG GTTTGCACGT CTACAGAAAC TGCATATGAG TGTGGCCGGA
    CGCAACCTTT ACATCCGTTT TCAGTCCAGG TCAGGTGATG CCATGGGGAT GAACATGATT
    TCAAAGGGTA CAGAGAAAGC ACTTTCCAAA CTTCAGGAGT ATTTCCCCGA AATGCAGATT
    CTGGCAGTTA GTGGGAACTA TTGTACAGAC AAGAAACCTG CGGCCATCAA CTGGATAGAG
    GGACGAGGAA AGTCTGTGGT CTGTGAAGCT GTCATTCCAG CCAAGGTTGT CAGAGAAGTA
    TTAAAGACTA CAACAGAGGC TATGATTGAG GTCAACATTA ATAAAAACCT GGTGGGCTCT
    GCCATGGCTG GGAGCATCGG CGGCTACAAC GCCCACGCAG CAAACATCGT AACTGCCATC
    TACATTGCCT GTGGACAGGA TGCAGCACAG AATGTTGGTA GTTCGAACTG TATTACTTTA
    ATGGAAGCAA GCGGTCCCAC GAACGAAGAC CTGTATATCA GCTGCACCAT GCCATCTATA
    GAAATAGGAA CCGTGGGCGG GGGGACCAAC TTGCTCCCTC AGCAGGCCTG CCTGCAGATG
    CTAGGCGTCC AAGGAGCGTG CAGAGACAAC CCCGGGGAAA ACGCGCGGCA GCTGGCCCGA
    ATCGTGTGCG GCACGGTGAT GGCGGGGGAG CTGTCCCTGA TGGCAGCACT GGCAGCAGGC
    CATCTGGTCA GAAGTCACAT GATCCACAAC AGGTCAAAGA TAAATTTGCA AGACCTCCAA
    GGAACTTGCA CTAAGAAGGC AGCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006074092.1
    CDS128..2794
    Translation

    Target ORF information:

    RefSeq Version XM_006074030.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis 3-hydroxy-3-methylglutaryl-CoA reductase (HMGCR), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006074030.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGTTGTCAA GACTTTTCCG AATGCACGGC CTCTTTGTGG CCTCCCATCC CTGGGAAGTC 
    ATAGTGGGGA CAGTGACGCT GACCATCTGC ATGATGTCCA TGAACATGTT TACTGGCAAC
    AATAAGATCT GTGGTTGGAA TTATGAATGT CCGAAGCTTG AAGAGGATGT TTTGAGCAGC
    GACATCATAA TTCTGACAAT AACACGATGC ATAGCAATAT TGTATATTTA CTTCCAGTTC
    CAGAATTTAC GCCAACTTGG ATCAAAATAT ATTTTGGGTA TTGCTGGGCT CTTCACAATT
    TTCTCCAGTT TCGTATTCAG TACAGTTGTC ATTCATGTCT TAGATAAAGA ATTGACAGGT
    TTGAATGAAG CTTTGCCCTT TTTCCTGCTG CTGGTCGACC TTTCTAGAGC AAGTGCGCTA
    GCCAAGTTCG CCCTCAGTTC TAACTCACAG GATGAAGTGA GGGAGAACAT TGCTCGTGGG
    ATGGCGATTT TAGGTCCCAC CTTCACCCTG GATGCTCTTG TAGAATGTCT CGTGATTGGA
    GTTGGTACCA TGTCAGGGGT GCGTCAGCTC GAAATCATGT GCTGCTTTGG CTGCATGTCG
    GTTCTCGCCA ACTACTTCGT GTTCATGACT TTCTTCCCAG CTTGTGTGTC CTTGGTTCTG
    GAGCTTTCTC GGGAAAGCCG CGAGGGCCGT CCAATCTGGC AGCTCAGCCA TTTTGCCCGA
    GTCTTAGAAG AAGAAGAAAA TAAGCCAAAT CCTGTTACTC AGAGGGTTAA GATGATTATG
    TCTCTAGGCT TGGTTCTTGT TCACGCTCAC AGTCGCTGGA TAGCTGATCC TTCTCCTCAA
    AACAGCACAG CAGACAATTC TAAGGTCTCT TTAGGATTGG ATGAAAATGT GTCCAAGAGA
    ATTGAACCGA GTGTTTCCCT CTGGCAGTTT TATCTCTCTA AAATGATCAG CATGGATATT
    GAACAAGTTA TTACCCTAAG TTTAGCTCTC CTTCTGGCTG TCAAGTATAT CTTCTTTGAA
    CAAGCAGAGA CAGAATCGAC ACTCTCGTTA AAAAATCCTA TCACATCTCC TGTAGTGACC
    CAAAAGAAAG TCACAGACGA TTGTTGTAGA CGTGACCCTG TACTGGTCAG AAATGACCAG
    AAATTCCATG CAATGGAGGA GGAGGCCAGG AAAAACAGAG AAAGAAAAGT TGAGGTTATA
    AAACCCTTAC TGGCAGAAAA TGACACCTCA AACAGAGCTA CATTTGTGGT GGGGAACTCC
    TCCTTACTCA GTACTTCACT GGAACTAGAG ACACAGGAAC CTGAAATTGA ACTTCCCGTG
    GAGCCTCGGC CTAATGAAGA GTGTCTGCAG ATTCTTGAGA ATGCAGAGAA AGGTGCAAAA
    TTCCTTAGTG ATGCTGAGAT CATCCAGTTG GTCAATGCAA AGCATATCCC AGCTTACAAA
    TTGGAAACTC TGATGGAAAC CCATGAGCGA GGTGTATCTA TTCGCCGACA GCTACTTTCC
    AAAAAACTTC CAGAGCCTTC TTCTCTCCAG TATCTACCTT ACAGGGACTA TAATTACTCC
    TTGGTGATGG GAGCTTGCTG TGAGAATGTT ATTGGATATA TGCCCATCCC TGTTGGAGTG
    GCAGGACCTC TGTGCTTGGA TGGAAAAGAA TTTCAGGTTC CAATGGCAAC AACAGAAGGC
    TGTCTCGTGG CCAGCACTAA TAGAGGCTGC AGAGCAATAG GCCTTGGTGG AGGTGCCAGC
    AGCCGGGTCC TTGCAGACGG GATGACTCGT GGCCCAGTGG TGCGTTTTCC CCGTGCTTGT
    GACTCTGCAG AAGTGAAGGC CTGGCTTGAG ACGCCTGAAG GGTTCACAGT GATAAAGGAA
    GCATTCGACA GCACCAGCAG GTTTGCACGT CTACAGAAAC TGCATATGAG TGTGGCCGGA
    CGCAACCTTT ACATCCGTTT TCAGTCCAGG TCAGGTGATG CCATGGGGAT GAACATGATT
    TCAAAGGGTA CAGAGAAAGC ACTTTCCAAA CTTCAGGAGT ATTTCCCCGA AATGCAGATT
    CTGGCAGTTA GTGGGAACTA TTGTACAGAC AAGAAACCTG CGGCCATCAA CTGGATAGAG
    GGACGAGGAA AGTCTGTGGT CTGTGAAGCT GTCATTCCAG CCAAGGTTGT CAGAGAAGTA
    TTAAAGACTA CAACAGAGGC TATGATTGAG GTCAACATTA ATAAAAACCT GGTGGGCTCT
    GCCATGGCTG GGAGCATCGG CGGCTACAAC GCCCACGCAG CAAACATCGT AACTGCCATC
    TACATTGCCT GTGGACAGGA TGCAGCACAG AATGTTGGTA GTTCGAACTG TATTACTTTA
    ATGGAAGCAA GCGGTCCCAC GAACGAAGAC CTGTATATCA GCTGCACCAT GCCATCTATA
    GAAATAGGAA CCGTGGGCGG GGGGACCAAC TTGCTCCCTC AGCAGGCCTG CCTGCAGATG
    CTAGGCGTCC AAGGAGCGTG CAGAGACAAC CCCGGGGAAA ACGCGCGGCA GCTGGCCCGA
    ATCGTGTGCG GCACGGTGAT GGCGGGGGAG CTGTCCCTGA TGGCAGCACT GGCAGCAGGC
    CATCTGGTCA GAAGTCACAT GATCCACAAC AGGTCAAAGA TAAATTTGCA AGACCTCCAA
    GGAACTTGCA CTAAGAAGGC AGCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu28003
    Clone ID Related Accession (Same CDS sequence) XM_006074030.1 , XM_006074029.2 , XM_006074029.1 , XM_006074032.1 , XM_006074031.2 , XM_006074030.2 , XM_025296388.1 , XM_006074031.1
    Accession Version XM_006074029.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2667bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product 3-hydroxy-3-methylglutaryl-Coenzyme A reductase isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037555.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006074029.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGTTGTCAA GACTTTTCCG AATGCACGGC CTCTTTGTGG CCTCCCATCC CTGGGAAGTC 
    ATAGTGGGGA CAGTGACGCT GACCATCTGC ATGATGTCCA TGAACATGTT TACTGGCAAC
    AATAAGATCT GTGGTTGGAA TTATGAATGT CCGAAGCTTG AAGAGGATGT TTTGAGCAGC
    GACATCATAA TTCTGACAAT AACACGATGC ATAGCAATAT TGTATATTTA CTTCCAGTTC
    CAGAATTTAC GCCAACTTGG ATCAAAATAT ATTTTGGGTA TTGCTGGGCT CTTCACAATT
    TTCTCCAGTT TCGTATTCAG TACAGTTGTC ATTCATGTCT TAGATAAAGA ATTGACAGGT
    TTGAATGAAG CTTTGCCCTT TTTCCTGCTG CTGGTCGACC TTTCTAGAGC AAGTGCGCTA
    GCCAAGTTCG CCCTCAGTTC TAACTCACAG GATGAAGTGA GGGAGAACAT TGCTCGTGGG
    ATGGCGATTT TAGGTCCCAC CTTCACCCTG GATGCTCTTG TAGAATGTCT CGTGATTGGA
    GTTGGTACCA TGTCAGGGGT GCGTCAGCTC GAAATCATGT GCTGCTTTGG CTGCATGTCG
    GTTCTCGCCA ACTACTTCGT GTTCATGACT TTCTTCCCAG CTTGTGTGTC CTTGGTTCTG
    GAGCTTTCTC GGGAAAGCCG CGAGGGCCGT CCAATCTGGC AGCTCAGCCA TTTTGCCCGA
    GTCTTAGAAG AAGAAGAAAA TAAGCCAAAT CCTGTTACTC AGAGGGTTAA GATGATTATG
    TCTCTAGGCT TGGTTCTTGT TCACGCTCAC AGTCGCTGGA TAGCTGATCC TTCTCCTCAA
    AACAGCACAG CAGACAATTC TAAGGTCTCT TTAGGATTGG ATGAAAATGT GTCCAAGAGA
    ATTGAACCGA GTGTTTCCCT CTGGCAGTTT TATCTCTCTA AAATGATCAG CATGGATATT
    GAACAAGTTA TTACCCTAAG TTTAGCTCTC CTTCTGGCTG TCAAGTATAT CTTCTTTGAA
    CAAGCAGAGA CAGAATCGAC ACTCTCGTTA AAAAATCCTA TCACATCTCC TGTAGTGACC
    CAAAAGAAAG TCACAGACGA TTGTTGTAGA CGTGACCCTG TACTGGTCAG AAATGACCAG
    AAATTCCATG CAATGGAGGA GGAGGCCAGG AAAAACAGAG AAAGAAAAGT TGAGGTTATA
    AAACCCTTAC TGGCAGAAAA TGACACCTCA AACAGAGCTA CATTTGTGGT GGGGAACTCC
    TCCTTACTCA GTACTTCACT GGAACTAGAG ACACAGGAAC CTGAAATTGA ACTTCCCGTG
    GAGCCTCGGC CTAATGAAGA GTGTCTGCAG ATTCTTGAGA ATGCAGAGAA AGGTGCAAAA
    TTCCTTAGTG ATGCTGAGAT CATCCAGTTG GTCAATGCAA AGCATATCCC AGCTTACAAA
    TTGGAAACTC TGATGGAAAC CCATGAGCGA GGTGTATCTA TTCGCCGACA GCTACTTTCC
    AAAAAACTTC CAGAGCCTTC TTCTCTCCAG TATCTACCTT ACAGGGACTA TAATTACTCC
    TTGGTGATGG GAGCTTGCTG TGAGAATGTT ATTGGATATA TGCCCATCCC TGTTGGAGTG
    GCAGGACCTC TGTGCTTGGA TGGAAAAGAA TTTCAGGTTC CAATGGCAAC AACAGAAGGC
    TGTCTCGTGG CCAGCACTAA TAGAGGCTGC AGAGCAATAG GCCTTGGTGG AGGTGCCAGC
    AGCCGGGTCC TTGCAGACGG GATGACTCGT GGCCCAGTGG TGCGTTTTCC CCGTGCTTGT
    GACTCTGCAG AAGTGAAGGC CTGGCTTGAG ACGCCTGAAG GGTTCACAGT GATAAAGGAA
    GCATTCGACA GCACCAGCAG GTTTGCACGT CTACAGAAAC TGCATATGAG TGTGGCCGGA
    CGCAACCTTT ACATCCGTTT TCAGTCCAGG TCAGGTGATG CCATGGGGAT GAACATGATT
    TCAAAGGGTA CAGAGAAAGC ACTTTCCAAA CTTCAGGAGT ATTTCCCCGA AATGCAGATT
    CTGGCAGTTA GTGGGAACTA TTGTACAGAC AAGAAACCTG CGGCCATCAA CTGGATAGAG
    GGACGAGGAA AGTCTGTGGT CTGTGAAGCT GTCATTCCAG CCAAGGTTGT CAGAGAAGTA
    TTAAAGACTA CAACAGAGGC TATGATTGAG GTCAACATTA ATAAAAACCT GGTGGGCTCT
    GCCATGGCTG GGAGCATCGG CGGCTACAAC GCCCACGCAG CAAACATCGT AACTGCCATC
    TACATTGCCT GTGGACAGGA TGCAGCACAG AATGTTGGTA GTTCGAACTG TATTACTTTA
    ATGGAAGCAA GCGGTCCCAC GAACGAAGAC CTGTATATCA GCTGCACCAT GCCATCTATA
    GAAATAGGAA CCGTGGGCGG GGGGACCAAC TTGCTCCCTC AGCAGGCCTG CCTGCAGATG
    CTAGGCGTCC AAGGAGCGTG CAGAGACAAC CCCGGGGAAA ACGCGCGGCA GCTGGCCCGA
    ATCGTGTGCG GCACGGTGAT GGCGGGGGAG CTGTCCCTGA TGGCAGCACT GGCAGCAGGC
    CATCTGGTCA GAAGTCACAT GATCCACAAC AGGTCAAAGA TAAATTTGCA AGACCTCCAA
    GGAACTTGCA CTAAGAAGGC AGCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006074091.1
    CDS156..2822
    Translation

    Target ORF information:

    RefSeq Version XM_006074029.2
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis 3-hydroxy-3-methylglutaryl-CoA reductase (HMGCR), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006074029.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGTTGTCAA GACTTTTCCG AATGCACGGC CTCTTTGTGG CCTCCCATCC CTGGGAAGTC 
    ATAGTGGGGA CAGTGACGCT GACCATCTGC ATGATGTCCA TGAACATGTT TACTGGCAAC
    AATAAGATCT GTGGTTGGAA TTATGAATGT CCGAAGCTTG AAGAGGATGT TTTGAGCAGC
    GACATCATAA TTCTGACAAT AACACGATGC ATAGCAATAT TGTATATTTA CTTCCAGTTC
    CAGAATTTAC GCCAACTTGG ATCAAAATAT ATTTTGGGTA TTGCTGGGCT CTTCACAATT
    TTCTCCAGTT TCGTATTCAG TACAGTTGTC ATTCATGTCT TAGATAAAGA ATTGACAGGT
    TTGAATGAAG CTTTGCCCTT TTTCCTGCTG CTGGTCGACC TTTCTAGAGC AAGTGCGCTA
    GCCAAGTTCG CCCTCAGTTC TAACTCACAG GATGAAGTGA GGGAGAACAT TGCTCGTGGG
    ATGGCGATTT TAGGTCCCAC CTTCACCCTG GATGCTCTTG TAGAATGTCT CGTGATTGGA
    GTTGGTACCA TGTCAGGGGT GCGTCAGCTC GAAATCATGT GCTGCTTTGG CTGCATGTCG
    GTTCTCGCCA ACTACTTCGT GTTCATGACT TTCTTCCCAG CTTGTGTGTC CTTGGTTCTG
    GAGCTTTCTC GGGAAAGCCG CGAGGGCCGT CCAATCTGGC AGCTCAGCCA TTTTGCCCGA
    GTCTTAGAAG AAGAAGAAAA TAAGCCAAAT CCTGTTACTC AGAGGGTTAA GATGATTATG
    TCTCTAGGCT TGGTTCTTGT TCACGCTCAC AGTCGCTGGA TAGCTGATCC TTCTCCTCAA
    AACAGCACAG CAGACAATTC TAAGGTCTCT TTAGGATTGG ATGAAAATGT GTCCAAGAGA
    ATTGAACCGA GTGTTTCCCT CTGGCAGTTT TATCTCTCTA AAATGATCAG CATGGATATT
    GAACAAGTTA TTACCCTAAG TTTAGCTCTC CTTCTGGCTG TCAAGTATAT CTTCTTTGAA
    CAAGCAGAGA CAGAATCGAC ACTCTCGTTA AAAAATCCTA TCACATCTCC TGTAGTGACC
    CAAAAGAAAG TCACAGACGA TTGTTGTAGA CGTGACCCTG TACTGGTCAG AAATGACCAG
    AAATTCCATG CAATGGAGGA GGAGGCCAGG AAAAACAGAG AAAGAAAAGT TGAGGTTATA
    AAACCCTTAC TGGCAGAAAA TGACACCTCA AACAGAGCTA CATTTGTGGT GGGGAACTCC
    TCCTTACTCA GTACTTCACT GGAACTAGAG ACACAGGAAC CTGAAATTGA ACTTCCCGTG
    GAGCCTCGGC CTAATGAAGA GTGTCTGCAG ATTCTTGAGA ATGCAGAGAA AGGTGCAAAA
    TTCCTTAGTG ATGCTGAGAT CATCCAGTTG GTCAATGCAA AGCATATCCC AGCTTACAAA
    TTGGAAACTC TGATGGAAAC CCATGAGCGA GGTGTATCTA TTCGCCGACA GCTACTTTCC
    AAAAAACTTC CAGAGCCTTC TTCTCTCCAG TATCTACCTT ACAGGGACTA TAATTACTCC
    TTGGTGATGG GAGCTTGCTG TGAGAATGTT ATTGGATATA TGCCCATCCC TGTTGGAGTG
    GCAGGACCTC TGTGCTTGGA TGGAAAAGAA TTTCAGGTTC CAATGGCAAC AACAGAAGGC
    TGTCTCGTGG CCAGCACTAA TAGAGGCTGC AGAGCAATAG GCCTTGGTGG AGGTGCCAGC
    AGCCGGGTCC TTGCAGACGG GATGACTCGT GGCCCAGTGG TGCGTTTTCC CCGTGCTTGT
    GACTCTGCAG AAGTGAAGGC CTGGCTTGAG ACGCCTGAAG GGTTCACAGT GATAAAGGAA
    GCATTCGACA GCACCAGCAG GTTTGCACGT CTACAGAAAC TGCATATGAG TGTGGCCGGA
    CGCAACCTTT ACATCCGTTT TCAGTCCAGG TCAGGTGATG CCATGGGGAT GAACATGATT
    TCAAAGGGTA CAGAGAAAGC ACTTTCCAAA CTTCAGGAGT ATTTCCCCGA AATGCAGATT
    CTGGCAGTTA GTGGGAACTA TTGTACAGAC AAGAAACCTG CGGCCATCAA CTGGATAGAG
    GGACGAGGAA AGTCTGTGGT CTGTGAAGCT GTCATTCCAG CCAAGGTTGT CAGAGAAGTA
    TTAAAGACTA CAACAGAGGC TATGATTGAG GTCAACATTA ATAAAAACCT GGTGGGCTCT
    GCCATGGCTG GGAGCATCGG CGGCTACAAC GCCCACGCAG CAAACATCGT AACTGCCATC
    TACATTGCCT GTGGACAGGA TGCAGCACAG AATGTTGGTA GTTCGAACTG TATTACTTTA
    ATGGAAGCAA GCGGTCCCAC GAACGAAGAC CTGTATATCA GCTGCACCAT GCCATCTATA
    GAAATAGGAA CCGTGGGCGG GGGGACCAAC TTGCTCCCTC AGCAGGCCTG CCTGCAGATG
    CTAGGCGTCC AAGGAGCGTG CAGAGACAAC CCCGGGGAAA ACGCGCGGCA GCTGGCCCGA
    ATCGTGTGCG GCACGGTGAT GGCGGGGGAG CTGTCCCTGA TGGCAGCACT GGCAGCAGGC
    CATCTGGTCA GAAGTCACAT GATCCACAAC AGGTCAAAGA TAAATTTGCA AGACCTCCAA
    GGAACTTGCA CTAAGAAGGC AGCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu28003
    Clone ID Related Accession (Same CDS sequence) XM_006074030.1 , XM_006074029.2 , XM_006074029.1 , XM_006074032.1 , XM_006074031.2 , XM_006074030.2 , XM_025296388.1 , XM_006074031.1
    Accession Version XM_006074029.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2667bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2014-03-11
    Organism Bubalus bubalis(water buffalo)
    Product 3-hydroxy-3-methylglutaryl-coenzyme A reductase isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785570.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGTTGTCAA GACTTTTCCG AATGCACGGC CTCTTTGTGG CCTCCCATCC CTGGGAAGTC 
    ATAGTGGGGA CAGTGACGCT GACCATCTGC ATGATGTCCA TGAACATGTT TACTGGCAAC
    AATAAGATCT GTGGTTGGAA TTATGAATGT CCGAAGCTTG AAGAGGATGT TTTGAGCAGC
    GACATCATAA TTCTGACAAT AACACGATGC ATAGCAATAT TGTATATTTA CTTCCAGTTC
    CAGAATTTAC GCCAACTTGG ATCAAAATAT ATTTTGGGTA TTGCTGGGCT CTTCACAATT
    TTCTCCAGTT TCGTATTCAG TACAGTTGTC ATTCATGTCT TAGATAAAGA ATTGACAGGT
    TTGAATGAAG CTTTGCCCTT TTTCCTGCTG CTGGTCGACC TTTCTAGAGC AAGTGCGCTA
    GCCAAGTTCG CCCTCAGTTC TAACTCACAG GATGAAGTGA GGGAGAACAT TGCTCGTGGG
    ATGGCGATTT TAGGTCCCAC CTTCACCCTG GATGCTCTTG TAGAATGTCT CGTGATTGGA
    GTTGGTACCA TGTCAGGGGT GCGTCAGCTC GAAATCATGT GCTGCTTTGG CTGCATGTCG
    GTTCTCGCCA ACTACTTCGT GTTCATGACT TTCTTCCCAG CTTGTGTGTC CTTGGTTCTG
    GAGCTTTCTC GGGAAAGCCG CGAGGGCCGT CCAATCTGGC AGCTCAGCCA TTTTGCCCGA
    GTCTTAGAAG AAGAAGAAAA TAAGCCAAAT CCTGTTACTC AGAGGGTTAA GATGATTATG
    TCTCTAGGCT TGGTTCTTGT TCACGCTCAC AGTCGCTGGA TAGCTGATCC TTCTCCTCAA
    AACAGCACAG CAGACAATTC TAAGGTCTCT TTAGGATTGG ATGAAAATGT GTCCAAGAGA
    ATTGAACCGA GTGTTTCCCT CTGGCAGTTT TATCTCTCTA AAATGATCAG CATGGATATT
    GAACAAGTTA TTACCCTAAG TTTAGCTCTC CTTCTGGCTG TCAAGTATAT CTTCTTTGAA
    CAAGCAGAGA CAGAATCGAC ACTCTCGTTA AAAAATCCTA TCACATCTCC TGTAGTGACC
    CAAAAGAAAG TCACAGACGA TTGTTGTAGA CGTGACCCTG TACTGGTCAG AAATGACCAG
    AAATTCCATG CAATGGAGGA GGAGGCCAGG AAAAACAGAG AAAGAAAAGT TGAGGTTATA
    AAACCCTTAC TGGCAGAAAA TGACACCTCA AACAGAGCTA CATTTGTGGT GGGGAACTCC
    TCCTTACTCA GTACTTCACT GGAACTAGAG ACACAGGAAC CTGAAATTGA ACTTCCCGTG
    GAGCCTCGGC CTAATGAAGA GTGTCTGCAG ATTCTTGAGA ATGCAGAGAA AGGTGCAAAA
    TTCCTTAGTG ATGCTGAGAT CATCCAGTTG GTCAATGCAA AGCATATCCC AGCTTACAAA
    TTGGAAACTC TGATGGAAAC CCATGAGCGA GGTGTATCTA TTCGCCGACA GCTACTTTCC
    AAAAAACTTC CAGAGCCTTC TTCTCTCCAG TATCTACCTT ACAGGGACTA TAATTACTCC
    TTGGTGATGG GAGCTTGCTG TGAGAATGTT ATTGGATATA TGCCCATCCC TGTTGGAGTG
    GCAGGACCTC TGTGCTTGGA TGGAAAAGAA TTTCAGGTTC CAATGGCAAC AACAGAAGGC
    TGTCTCGTGG CCAGCACTAA TAGAGGCTGC AGAGCAATAG GCCTTGGTGG AGGTGCCAGC
    AGCCGGGTCC TTGCAGACGG GATGACTCGT GGCCCAGTGG TGCGTTTTCC CCGTGCTTGT
    GACTCTGCAG AAGTGAAGGC CTGGCTTGAG ACGCCTGAAG GGTTCACAGT GATAAAGGAA
    GCATTCGACA GCACCAGCAG GTTTGCACGT CTACAGAAAC TGCATATGAG TGTGGCCGGA
    CGCAACCTTT ACATCCGTTT TCAGTCCAGG TCAGGTGATG CCATGGGGAT GAACATGATT
    TCAAAGGGTA CAGAGAAAGC ACTTTCCAAA CTTCAGGAGT ATTTCCCCGA AATGCAGATT
    CTGGCAGTTA GTGGGAACTA TTGTACAGAC AAGAAACCTG CGGCCATCAA CTGGATAGAG
    GGACGAGGAA AGTCTGTGGT CTGTGAAGCT GTCATTCCAG CCAAGGTTGT CAGAGAAGTA
    TTAAAGACTA CAACAGAGGC TATGATTGAG GTCAACATTA ATAAAAACCT GGTGGGCTCT
    GCCATGGCTG GGAGCATCGG CGGCTACAAC GCCCACGCAG CAAACATCGT AACTGCCATC
    TACATTGCCT GTGGACAGGA TGCAGCACAG AATGTTGGTA GTTCGAACTG TATTACTTTA
    ATGGAAGCAA GCGGTCCCAC GAACGAAGAC CTGTATATCA GCTGCACCAT GCCATCTATA
    GAAATAGGAA CCGTGGGCGG GGGGACCAAC TTGCTCCCTC AGCAGGCCTG CCTGCAGATG
    CTAGGCGTCC AAGGAGCGTG CAGAGACAAC CCCGGGGAAA ACGCGCGGCA GCTGGCCCGA
    ATCGTGTGCG GCACGGTGAT GGCGGGGGAG CTGTCCCTGA TGGCAGCACT GGCAGCAGGC
    CATCTGGTCA GAAGTCACAT GATCCACAAC AGGTCAAAGA TAAATTTGCA AGACCTCCAA
    GGAACTTGCA CTAAGAAGGC AGCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006074091.1
    CDS126..2792
    Translation

    Target ORF information:

    RefSeq Version XM_006074029.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis 3-hydroxy-3-methylglutaryl-CoA reductase (HMGCR), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006074029.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGTTGTCAA GACTTTTCCG AATGCACGGC CTCTTTGTGG CCTCCCATCC CTGGGAAGTC 
    ATAGTGGGGA CAGTGACGCT GACCATCTGC ATGATGTCCA TGAACATGTT TACTGGCAAC
    AATAAGATCT GTGGTTGGAA TTATGAATGT CCGAAGCTTG AAGAGGATGT TTTGAGCAGC
    GACATCATAA TTCTGACAAT AACACGATGC ATAGCAATAT TGTATATTTA CTTCCAGTTC
    CAGAATTTAC GCCAACTTGG ATCAAAATAT ATTTTGGGTA TTGCTGGGCT CTTCACAATT
    TTCTCCAGTT TCGTATTCAG TACAGTTGTC ATTCATGTCT TAGATAAAGA ATTGACAGGT
    TTGAATGAAG CTTTGCCCTT TTTCCTGCTG CTGGTCGACC TTTCTAGAGC AAGTGCGCTA
    GCCAAGTTCG CCCTCAGTTC TAACTCACAG GATGAAGTGA GGGAGAACAT TGCTCGTGGG
    ATGGCGATTT TAGGTCCCAC CTTCACCCTG GATGCTCTTG TAGAATGTCT CGTGATTGGA
    GTTGGTACCA TGTCAGGGGT GCGTCAGCTC GAAATCATGT GCTGCTTTGG CTGCATGTCG
    GTTCTCGCCA ACTACTTCGT GTTCATGACT TTCTTCCCAG CTTGTGTGTC CTTGGTTCTG
    GAGCTTTCTC GGGAAAGCCG CGAGGGCCGT CCAATCTGGC AGCTCAGCCA TTTTGCCCGA
    GTCTTAGAAG AAGAAGAAAA TAAGCCAAAT CCTGTTACTC AGAGGGTTAA GATGATTATG
    TCTCTAGGCT TGGTTCTTGT TCACGCTCAC AGTCGCTGGA TAGCTGATCC TTCTCCTCAA
    AACAGCACAG CAGACAATTC TAAGGTCTCT TTAGGATTGG ATGAAAATGT GTCCAAGAGA
    ATTGAACCGA GTGTTTCCCT CTGGCAGTTT TATCTCTCTA AAATGATCAG CATGGATATT
    GAACAAGTTA TTACCCTAAG TTTAGCTCTC CTTCTGGCTG TCAAGTATAT CTTCTTTGAA
    CAAGCAGAGA CAGAATCGAC ACTCTCGTTA AAAAATCCTA TCACATCTCC TGTAGTGACC
    CAAAAGAAAG TCACAGACGA TTGTTGTAGA CGTGACCCTG TACTGGTCAG AAATGACCAG
    AAATTCCATG CAATGGAGGA GGAGGCCAGG AAAAACAGAG AAAGAAAAGT TGAGGTTATA
    AAACCCTTAC TGGCAGAAAA TGACACCTCA AACAGAGCTA CATTTGTGGT GGGGAACTCC
    TCCTTACTCA GTACTTCACT GGAACTAGAG ACACAGGAAC CTGAAATTGA ACTTCCCGTG
    GAGCCTCGGC CTAATGAAGA GTGTCTGCAG ATTCTTGAGA ATGCAGAGAA AGGTGCAAAA
    TTCCTTAGTG ATGCTGAGAT CATCCAGTTG GTCAATGCAA AGCATATCCC AGCTTACAAA
    TTGGAAACTC TGATGGAAAC CCATGAGCGA GGTGTATCTA TTCGCCGACA GCTACTTTCC
    AAAAAACTTC CAGAGCCTTC TTCTCTCCAG TATCTACCTT ACAGGGACTA TAATTACTCC
    TTGGTGATGG GAGCTTGCTG TGAGAATGTT ATTGGATATA TGCCCATCCC TGTTGGAGTG
    GCAGGACCTC TGTGCTTGGA TGGAAAAGAA TTTCAGGTTC CAATGGCAAC AACAGAAGGC
    TGTCTCGTGG CCAGCACTAA TAGAGGCTGC AGAGCAATAG GCCTTGGTGG AGGTGCCAGC
    AGCCGGGTCC TTGCAGACGG GATGACTCGT GGCCCAGTGG TGCGTTTTCC CCGTGCTTGT
    GACTCTGCAG AAGTGAAGGC CTGGCTTGAG ACGCCTGAAG GGTTCACAGT GATAAAGGAA
    GCATTCGACA GCACCAGCAG GTTTGCACGT CTACAGAAAC TGCATATGAG TGTGGCCGGA
    CGCAACCTTT ACATCCGTTT TCAGTCCAGG TCAGGTGATG CCATGGGGAT GAACATGATT
    TCAAAGGGTA CAGAGAAAGC ACTTTCCAAA CTTCAGGAGT ATTTCCCCGA AATGCAGATT
    CTGGCAGTTA GTGGGAACTA TTGTACAGAC AAGAAACCTG CGGCCATCAA CTGGATAGAG
    GGACGAGGAA AGTCTGTGGT CTGTGAAGCT GTCATTCCAG CCAAGGTTGT CAGAGAAGTA
    TTAAAGACTA CAACAGAGGC TATGATTGAG GTCAACATTA ATAAAAACCT GGTGGGCTCT
    GCCATGGCTG GGAGCATCGG CGGCTACAAC GCCCACGCAG CAAACATCGT AACTGCCATC
    TACATTGCCT GTGGACAGGA TGCAGCACAG AATGTTGGTA GTTCGAACTG TATTACTTTA
    ATGGAAGCAA GCGGTCCCAC GAACGAAGAC CTGTATATCA GCTGCACCAT GCCATCTATA
    GAAATAGGAA CCGTGGGCGG GGGGACCAAC TTGCTCCCTC AGCAGGCCTG CCTGCAGATG
    CTAGGCGTCC AAGGAGCGTG CAGAGACAAC CCCGGGGAAA ACGCGCGGCA GCTGGCCCGA
    ATCGTGTGCG GCACGGTGAT GGCGGGGGAG CTGTCCCTGA TGGCAGCACT GGCAGCAGGC
    CATCTGGTCA GAAGTCACAT GATCCACAAC AGGTCAAAGA TAAATTTGCA AGACCTCCAA
    GGAACTTGCA CTAAGAAGGC AGCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu28003
    Clone ID Related Accession (Same CDS sequence) XM_006074030.1 , XM_006074029.2 , XM_006074029.1 , XM_006074032.1 , XM_006074031.2 , XM_006074030.2 , XM_025296388.1 , XM_006074031.1
    Accession Version XM_006074032.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2667bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2014-03-11
    Organism Bubalus bubalis(water buffalo)
    Product 3-hydroxy-3-methylglutaryl-coenzyme A reductase isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785570.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGTTGTCAA GACTTTTCCG AATGCACGGC CTCTTTGTGG CCTCCCATCC CTGGGAAGTC 
    ATAGTGGGGA CAGTGACGCT GACCATCTGC ATGATGTCCA TGAACATGTT TACTGGCAAC
    AATAAGATCT GTGGTTGGAA TTATGAATGT CCGAAGCTTG AAGAGGATGT TTTGAGCAGC
    GACATCATAA TTCTGACAAT AACACGATGC ATAGCAATAT TGTATATTTA CTTCCAGTTC
    CAGAATTTAC GCCAACTTGG ATCAAAATAT ATTTTGGGTA TTGCTGGGCT CTTCACAATT
    TTCTCCAGTT TCGTATTCAG TACAGTTGTC ATTCATGTCT TAGATAAAGA ATTGACAGGT
    TTGAATGAAG CTTTGCCCTT TTTCCTGCTG CTGGTCGACC TTTCTAGAGC AAGTGCGCTA
    GCCAAGTTCG CCCTCAGTTC TAACTCACAG GATGAAGTGA GGGAGAACAT TGCTCGTGGG
    ATGGCGATTT TAGGTCCCAC CTTCACCCTG GATGCTCTTG TAGAATGTCT CGTGATTGGA
    GTTGGTACCA TGTCAGGGGT GCGTCAGCTC GAAATCATGT GCTGCTTTGG CTGCATGTCG
    GTTCTCGCCA ACTACTTCGT GTTCATGACT TTCTTCCCAG CTTGTGTGTC CTTGGTTCTG
    GAGCTTTCTC GGGAAAGCCG CGAGGGCCGT CCAATCTGGC AGCTCAGCCA TTTTGCCCGA
    GTCTTAGAAG AAGAAGAAAA TAAGCCAAAT CCTGTTACTC AGAGGGTTAA GATGATTATG
    TCTCTAGGCT TGGTTCTTGT TCACGCTCAC AGTCGCTGGA TAGCTGATCC TTCTCCTCAA
    AACAGCACAG CAGACAATTC TAAGGTCTCT TTAGGATTGG ATGAAAATGT GTCCAAGAGA
    ATTGAACCGA GTGTTTCCCT CTGGCAGTTT TATCTCTCTA AAATGATCAG CATGGATATT
    GAACAAGTTA TTACCCTAAG TTTAGCTCTC CTTCTGGCTG TCAAGTATAT CTTCTTTGAA
    CAAGCAGAGA CAGAATCGAC ACTCTCGTTA AAAAATCCTA TCACATCTCC TGTAGTGACC
    CAAAAGAAAG TCACAGACGA TTGTTGTAGA CGTGACCCTG TACTGGTCAG AAATGACCAG
    AAATTCCATG CAATGGAGGA GGAGGCCAGG AAAAACAGAG AAAGAAAAGT TGAGGTTATA
    AAACCCTTAC TGGCAGAAAA TGACACCTCA AACAGAGCTA CATTTGTGGT GGGGAACTCC
    TCCTTACTCA GTACTTCACT GGAACTAGAG ACACAGGAAC CTGAAATTGA ACTTCCCGTG
    GAGCCTCGGC CTAATGAAGA GTGTCTGCAG ATTCTTGAGA ATGCAGAGAA AGGTGCAAAA
    TTCCTTAGTG ATGCTGAGAT CATCCAGTTG GTCAATGCAA AGCATATCCC AGCTTACAAA
    TTGGAAACTC TGATGGAAAC CCATGAGCGA GGTGTATCTA TTCGCCGACA GCTACTTTCC
    AAAAAACTTC CAGAGCCTTC TTCTCTCCAG TATCTACCTT ACAGGGACTA TAATTACTCC
    TTGGTGATGG GAGCTTGCTG TGAGAATGTT ATTGGATATA TGCCCATCCC TGTTGGAGTG
    GCAGGACCTC TGTGCTTGGA TGGAAAAGAA TTTCAGGTTC CAATGGCAAC AACAGAAGGC
    TGTCTCGTGG CCAGCACTAA TAGAGGCTGC AGAGCAATAG GCCTTGGTGG AGGTGCCAGC
    AGCCGGGTCC TTGCAGACGG GATGACTCGT GGCCCAGTGG TGCGTTTTCC CCGTGCTTGT
    GACTCTGCAG AAGTGAAGGC CTGGCTTGAG ACGCCTGAAG GGTTCACAGT GATAAAGGAA
    GCATTCGACA GCACCAGCAG GTTTGCACGT CTACAGAAAC TGCATATGAG TGTGGCCGGA
    CGCAACCTTT ACATCCGTTT TCAGTCCAGG TCAGGTGATG CCATGGGGAT GAACATGATT
    TCAAAGGGTA CAGAGAAAGC ACTTTCCAAA CTTCAGGAGT ATTTCCCCGA AATGCAGATT
    CTGGCAGTTA GTGGGAACTA TTGTACAGAC AAGAAACCTG CGGCCATCAA CTGGATAGAG
    GGACGAGGAA AGTCTGTGGT CTGTGAAGCT GTCATTCCAG CCAAGGTTGT CAGAGAAGTA
    TTAAAGACTA CAACAGAGGC TATGATTGAG GTCAACATTA ATAAAAACCT GGTGGGCTCT
    GCCATGGCTG GGAGCATCGG CGGCTACAAC GCCCACGCAG CAAACATCGT AACTGCCATC
    TACATTGCCT GTGGACAGGA TGCAGCACAG AATGTTGGTA GTTCGAACTG TATTACTTTA
    ATGGAAGCAA GCGGTCCCAC GAACGAAGAC CTGTATATCA GCTGCACCAT GCCATCTATA
    GAAATAGGAA CCGTGGGCGG GGGGACCAAC TTGCTCCCTC AGCAGGCCTG CCTGCAGATG
    CTAGGCGTCC AAGGAGCGTG CAGAGACAAC CCCGGGGAAA ACGCGCGGCA GCTGGCCCGA
    ATCGTGTGCG GCACGGTGAT GGCGGGGGAG CTGTCCCTGA TGGCAGCACT GGCAGCAGGC
    CATCTGGTCA GAAGTCACAT GATCCACAAC AGGTCAAAGA TAAATTTGCA AGACCTCCAA
    GGAACTTGCA CTAAGAAGGC AGCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006074094.1
    CDS47..2713
    Translation

    Target ORF information:

    RefSeq Version XM_006074032.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis 3-hydroxy-3-methylglutaryl-CoA reductase (HMGCR), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006074032.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGTTGTCAA GACTTTTCCG AATGCACGGC CTCTTTGTGG CCTCCCATCC CTGGGAAGTC 
    ATAGTGGGGA CAGTGACGCT GACCATCTGC ATGATGTCCA TGAACATGTT TACTGGCAAC
    AATAAGATCT GTGGTTGGAA TTATGAATGT CCGAAGCTTG AAGAGGATGT TTTGAGCAGC
    GACATCATAA TTCTGACAAT AACACGATGC ATAGCAATAT TGTATATTTA CTTCCAGTTC
    CAGAATTTAC GCCAACTTGG ATCAAAATAT ATTTTGGGTA TTGCTGGGCT CTTCACAATT
    TTCTCCAGTT TCGTATTCAG TACAGTTGTC ATTCATGTCT TAGATAAAGA ATTGACAGGT
    TTGAATGAAG CTTTGCCCTT TTTCCTGCTG CTGGTCGACC TTTCTAGAGC AAGTGCGCTA
    GCCAAGTTCG CCCTCAGTTC TAACTCACAG GATGAAGTGA GGGAGAACAT TGCTCGTGGG
    ATGGCGATTT TAGGTCCCAC CTTCACCCTG GATGCTCTTG TAGAATGTCT CGTGATTGGA
    GTTGGTACCA TGTCAGGGGT GCGTCAGCTC GAAATCATGT GCTGCTTTGG CTGCATGTCG
    GTTCTCGCCA ACTACTTCGT GTTCATGACT TTCTTCCCAG CTTGTGTGTC CTTGGTTCTG
    GAGCTTTCTC GGGAAAGCCG CGAGGGCCGT CCAATCTGGC AGCTCAGCCA TTTTGCCCGA
    GTCTTAGAAG AAGAAGAAAA TAAGCCAAAT CCTGTTACTC AGAGGGTTAA GATGATTATG
    TCTCTAGGCT TGGTTCTTGT TCACGCTCAC AGTCGCTGGA TAGCTGATCC TTCTCCTCAA
    AACAGCACAG CAGACAATTC TAAGGTCTCT TTAGGATTGG ATGAAAATGT GTCCAAGAGA
    ATTGAACCGA GTGTTTCCCT CTGGCAGTTT TATCTCTCTA AAATGATCAG CATGGATATT
    GAACAAGTTA TTACCCTAAG TTTAGCTCTC CTTCTGGCTG TCAAGTATAT CTTCTTTGAA
    CAAGCAGAGA CAGAATCGAC ACTCTCGTTA AAAAATCCTA TCACATCTCC TGTAGTGACC
    CAAAAGAAAG TCACAGACGA TTGTTGTAGA CGTGACCCTG TACTGGTCAG AAATGACCAG
    AAATTCCATG CAATGGAGGA GGAGGCCAGG AAAAACAGAG AAAGAAAAGT TGAGGTTATA
    AAACCCTTAC TGGCAGAAAA TGACACCTCA AACAGAGCTA CATTTGTGGT GGGGAACTCC
    TCCTTACTCA GTACTTCACT GGAACTAGAG ACACAGGAAC CTGAAATTGA ACTTCCCGTG
    GAGCCTCGGC CTAATGAAGA GTGTCTGCAG ATTCTTGAGA ATGCAGAGAA AGGTGCAAAA
    TTCCTTAGTG ATGCTGAGAT CATCCAGTTG GTCAATGCAA AGCATATCCC AGCTTACAAA
    TTGGAAACTC TGATGGAAAC CCATGAGCGA GGTGTATCTA TTCGCCGACA GCTACTTTCC
    AAAAAACTTC CAGAGCCTTC TTCTCTCCAG TATCTACCTT ACAGGGACTA TAATTACTCC
    TTGGTGATGG GAGCTTGCTG TGAGAATGTT ATTGGATATA TGCCCATCCC TGTTGGAGTG
    GCAGGACCTC TGTGCTTGGA TGGAAAAGAA TTTCAGGTTC CAATGGCAAC AACAGAAGGC
    TGTCTCGTGG CCAGCACTAA TAGAGGCTGC AGAGCAATAG GCCTTGGTGG AGGTGCCAGC
    AGCCGGGTCC TTGCAGACGG GATGACTCGT GGCCCAGTGG TGCGTTTTCC CCGTGCTTGT
    GACTCTGCAG AAGTGAAGGC CTGGCTTGAG ACGCCTGAAG GGTTCACAGT GATAAAGGAA
    GCATTCGACA GCACCAGCAG GTTTGCACGT CTACAGAAAC TGCATATGAG TGTGGCCGGA
    CGCAACCTTT ACATCCGTTT TCAGTCCAGG TCAGGTGATG CCATGGGGAT GAACATGATT
    TCAAAGGGTA CAGAGAAAGC ACTTTCCAAA CTTCAGGAGT ATTTCCCCGA AATGCAGATT
    CTGGCAGTTA GTGGGAACTA TTGTACAGAC AAGAAACCTG CGGCCATCAA CTGGATAGAG
    GGACGAGGAA AGTCTGTGGT CTGTGAAGCT GTCATTCCAG CCAAGGTTGT CAGAGAAGTA
    TTAAAGACTA CAACAGAGGC TATGATTGAG GTCAACATTA ATAAAAACCT GGTGGGCTCT
    GCCATGGCTG GGAGCATCGG CGGCTACAAC GCCCACGCAG CAAACATCGT AACTGCCATC
    TACATTGCCT GTGGACAGGA TGCAGCACAG AATGTTGGTA GTTCGAACTG TATTACTTTA
    ATGGAAGCAA GCGGTCCCAC GAACGAAGAC CTGTATATCA GCTGCACCAT GCCATCTATA
    GAAATAGGAA CCGTGGGCGG GGGGACCAAC TTGCTCCCTC AGCAGGCCTG CCTGCAGATG
    CTAGGCGTCC AAGGAGCGTG CAGAGACAAC CCCGGGGAAA ACGCGCGGCA GCTGGCCCGA
    ATCGTGTGCG GCACGGTGAT GGCGGGGGAG CTGTCCCTGA TGGCAGCACT GGCAGCAGGC
    CATCTGGTCA GAAGTCACAT GATCCACAAC AGGTCAAAGA TAAATTTGCA AGACCTCCAA
    GGAACTTGCA CTAAGAAGGC AGCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu28003
    Clone ID Related Accession (Same CDS sequence) XM_006074030.1 , XM_006074029.2 , XM_006074029.1 , XM_006074032.1 , XM_006074031.2 , XM_006074030.2 , XM_025296388.1 , XM_006074031.1
    Accession Version XM_006074031.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2667bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product 3-hydroxy-3-methylglutaryl-Coenzyme A reductase isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037555.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006074031.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGTTGTCAA GACTTTTCCG AATGCACGGC CTCTTTGTGG CCTCCCATCC CTGGGAAGTC 
    ATAGTGGGGA CAGTGACGCT GACCATCTGC ATGATGTCCA TGAACATGTT TACTGGCAAC
    AATAAGATCT GTGGTTGGAA TTATGAATGT CCGAAGCTTG AAGAGGATGT TTTGAGCAGC
    GACATCATAA TTCTGACAAT AACACGATGC ATAGCAATAT TGTATATTTA CTTCCAGTTC
    CAGAATTTAC GCCAACTTGG ATCAAAATAT ATTTTGGGTA TTGCTGGGCT CTTCACAATT
    TTCTCCAGTT TCGTATTCAG TACAGTTGTC ATTCATGTCT TAGATAAAGA ATTGACAGGT
    TTGAATGAAG CTTTGCCCTT TTTCCTGCTG CTGGTCGACC TTTCTAGAGC AAGTGCGCTA
    GCCAAGTTCG CCCTCAGTTC TAACTCACAG GATGAAGTGA GGGAGAACAT TGCTCGTGGG
    ATGGCGATTT TAGGTCCCAC CTTCACCCTG GATGCTCTTG TAGAATGTCT CGTGATTGGA
    GTTGGTACCA TGTCAGGGGT GCGTCAGCTC GAAATCATGT GCTGCTTTGG CTGCATGTCG
    GTTCTCGCCA ACTACTTCGT GTTCATGACT TTCTTCCCAG CTTGTGTGTC CTTGGTTCTG
    GAGCTTTCTC GGGAAAGCCG CGAGGGCCGT CCAATCTGGC AGCTCAGCCA TTTTGCCCGA
    GTCTTAGAAG AAGAAGAAAA TAAGCCAAAT CCTGTTACTC AGAGGGTTAA GATGATTATG
    TCTCTAGGCT TGGTTCTTGT TCACGCTCAC AGTCGCTGGA TAGCTGATCC TTCTCCTCAA
    AACAGCACAG CAGACAATTC TAAGGTCTCT TTAGGATTGG ATGAAAATGT GTCCAAGAGA
    ATTGAACCGA GTGTTTCCCT CTGGCAGTTT TATCTCTCTA AAATGATCAG CATGGATATT
    GAACAAGTTA TTACCCTAAG TTTAGCTCTC CTTCTGGCTG TCAAGTATAT CTTCTTTGAA
    CAAGCAGAGA CAGAATCGAC ACTCTCGTTA AAAAATCCTA TCACATCTCC TGTAGTGACC
    CAAAAGAAAG TCACAGACGA TTGTTGTAGA CGTGACCCTG TACTGGTCAG AAATGACCAG
    AAATTCCATG CAATGGAGGA GGAGGCCAGG AAAAACAGAG AAAGAAAAGT TGAGGTTATA
    AAACCCTTAC TGGCAGAAAA TGACACCTCA AACAGAGCTA CATTTGTGGT GGGGAACTCC
    TCCTTACTCA GTACTTCACT GGAACTAGAG ACACAGGAAC CTGAAATTGA ACTTCCCGTG
    GAGCCTCGGC CTAATGAAGA GTGTCTGCAG ATTCTTGAGA ATGCAGAGAA AGGTGCAAAA
    TTCCTTAGTG ATGCTGAGAT CATCCAGTTG GTCAATGCAA AGCATATCCC AGCTTACAAA
    TTGGAAACTC TGATGGAAAC CCATGAGCGA GGTGTATCTA TTCGCCGACA GCTACTTTCC
    AAAAAACTTC CAGAGCCTTC TTCTCTCCAG TATCTACCTT ACAGGGACTA TAATTACTCC
    TTGGTGATGG GAGCTTGCTG TGAGAATGTT ATTGGATATA TGCCCATCCC TGTTGGAGTG
    GCAGGACCTC TGTGCTTGGA TGGAAAAGAA TTTCAGGTTC CAATGGCAAC AACAGAAGGC
    TGTCTCGTGG CCAGCACTAA TAGAGGCTGC AGAGCAATAG GCCTTGGTGG AGGTGCCAGC
    AGCCGGGTCC TTGCAGACGG GATGACTCGT GGCCCAGTGG TGCGTTTTCC CCGTGCTTGT
    GACTCTGCAG AAGTGAAGGC CTGGCTTGAG ACGCCTGAAG GGTTCACAGT GATAAAGGAA
    GCATTCGACA GCACCAGCAG GTTTGCACGT CTACAGAAAC TGCATATGAG TGTGGCCGGA
    CGCAACCTTT ACATCCGTTT TCAGTCCAGG TCAGGTGATG CCATGGGGAT GAACATGATT
    TCAAAGGGTA CAGAGAAAGC ACTTTCCAAA CTTCAGGAGT ATTTCCCCGA AATGCAGATT
    CTGGCAGTTA GTGGGAACTA TTGTACAGAC AAGAAACCTG CGGCCATCAA CTGGATAGAG
    GGACGAGGAA AGTCTGTGGT CTGTGAAGCT GTCATTCCAG CCAAGGTTGT CAGAGAAGTA
    TTAAAGACTA CAACAGAGGC TATGATTGAG GTCAACATTA ATAAAAACCT GGTGGGCTCT
    GCCATGGCTG GGAGCATCGG CGGCTACAAC GCCCACGCAG CAAACATCGT AACTGCCATC
    TACATTGCCT GTGGACAGGA TGCAGCACAG AATGTTGGTA GTTCGAACTG TATTACTTTA
    ATGGAAGCAA GCGGTCCCAC GAACGAAGAC CTGTATATCA GCTGCACCAT GCCATCTATA
    GAAATAGGAA CCGTGGGCGG GGGGACCAAC TTGCTCCCTC AGCAGGCCTG CCTGCAGATG
    CTAGGCGTCC AAGGAGCGTG CAGAGACAAC CCCGGGGAAA ACGCGCGGCA GCTGGCCCGA
    ATCGTGTGCG GCACGGTGAT GGCGGGGGAG CTGTCCCTGA TGGCAGCACT GGCAGCAGGC
    CATCTGGTCA GAAGTCACAT GATCCACAAC AGGTCAAAGA TAAATTTGCA AGACCTCCAA
    GGAACTTGCA CTAAGAAGGC AGCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006074093.1
    CDS1352..4018
    Translation

    Target ORF information:

    RefSeq Version XM_006074031.2
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis 3-hydroxy-3-methylglutaryl-CoA reductase (HMGCR), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006074031.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGTTGTCAA GACTTTTCCG AATGCACGGC CTCTTTGTGG CCTCCCATCC CTGGGAAGTC 
    ATAGTGGGGA CAGTGACGCT GACCATCTGC ATGATGTCCA TGAACATGTT TACTGGCAAC
    AATAAGATCT GTGGTTGGAA TTATGAATGT CCGAAGCTTG AAGAGGATGT TTTGAGCAGC
    GACATCATAA TTCTGACAAT AACACGATGC ATAGCAATAT TGTATATTTA CTTCCAGTTC
    CAGAATTTAC GCCAACTTGG ATCAAAATAT ATTTTGGGTA TTGCTGGGCT CTTCACAATT
    TTCTCCAGTT TCGTATTCAG TACAGTTGTC ATTCATGTCT TAGATAAAGA ATTGACAGGT
    TTGAATGAAG CTTTGCCCTT TTTCCTGCTG CTGGTCGACC TTTCTAGAGC AAGTGCGCTA
    GCCAAGTTCG CCCTCAGTTC TAACTCACAG GATGAAGTGA GGGAGAACAT TGCTCGTGGG
    ATGGCGATTT TAGGTCCCAC CTTCACCCTG GATGCTCTTG TAGAATGTCT CGTGATTGGA
    GTTGGTACCA TGTCAGGGGT GCGTCAGCTC GAAATCATGT GCTGCTTTGG CTGCATGTCG
    GTTCTCGCCA ACTACTTCGT GTTCATGACT TTCTTCCCAG CTTGTGTGTC CTTGGTTCTG
    GAGCTTTCTC GGGAAAGCCG CGAGGGCCGT CCAATCTGGC AGCTCAGCCA TTTTGCCCGA
    GTCTTAGAAG AAGAAGAAAA TAAGCCAAAT CCTGTTACTC AGAGGGTTAA GATGATTATG
    TCTCTAGGCT TGGTTCTTGT TCACGCTCAC AGTCGCTGGA TAGCTGATCC TTCTCCTCAA
    AACAGCACAG CAGACAATTC TAAGGTCTCT TTAGGATTGG ATGAAAATGT GTCCAAGAGA
    ATTGAACCGA GTGTTTCCCT CTGGCAGTTT TATCTCTCTA AAATGATCAG CATGGATATT
    GAACAAGTTA TTACCCTAAG TTTAGCTCTC CTTCTGGCTG TCAAGTATAT CTTCTTTGAA
    CAAGCAGAGA CAGAATCGAC ACTCTCGTTA AAAAATCCTA TCACATCTCC TGTAGTGACC
    CAAAAGAAAG TCACAGACGA TTGTTGTAGA CGTGACCCTG TACTGGTCAG AAATGACCAG
    AAATTCCATG CAATGGAGGA GGAGGCCAGG AAAAACAGAG AAAGAAAAGT TGAGGTTATA
    AAACCCTTAC TGGCAGAAAA TGACACCTCA AACAGAGCTA CATTTGTGGT GGGGAACTCC
    TCCTTACTCA GTACTTCACT GGAACTAGAG ACACAGGAAC CTGAAATTGA ACTTCCCGTG
    GAGCCTCGGC CTAATGAAGA GTGTCTGCAG ATTCTTGAGA ATGCAGAGAA AGGTGCAAAA
    TTCCTTAGTG ATGCTGAGAT CATCCAGTTG GTCAATGCAA AGCATATCCC AGCTTACAAA
    TTGGAAACTC TGATGGAAAC CCATGAGCGA GGTGTATCTA TTCGCCGACA GCTACTTTCC
    AAAAAACTTC CAGAGCCTTC TTCTCTCCAG TATCTACCTT ACAGGGACTA TAATTACTCC
    TTGGTGATGG GAGCTTGCTG TGAGAATGTT ATTGGATATA TGCCCATCCC TGTTGGAGTG
    GCAGGACCTC TGTGCTTGGA TGGAAAAGAA TTTCAGGTTC CAATGGCAAC AACAGAAGGC
    TGTCTCGTGG CCAGCACTAA TAGAGGCTGC AGAGCAATAG GCCTTGGTGG AGGTGCCAGC
    AGCCGGGTCC TTGCAGACGG GATGACTCGT GGCCCAGTGG TGCGTTTTCC CCGTGCTTGT
    GACTCTGCAG AAGTGAAGGC CTGGCTTGAG ACGCCTGAAG GGTTCACAGT GATAAAGGAA
    GCATTCGACA GCACCAGCAG GTTTGCACGT CTACAGAAAC TGCATATGAG TGTGGCCGGA
    CGCAACCTTT ACATCCGTTT TCAGTCCAGG TCAGGTGATG CCATGGGGAT GAACATGATT
    TCAAAGGGTA CAGAGAAAGC ACTTTCCAAA CTTCAGGAGT ATTTCCCCGA AATGCAGATT
    CTGGCAGTTA GTGGGAACTA TTGTACAGAC AAGAAACCTG CGGCCATCAA CTGGATAGAG
    GGACGAGGAA AGTCTGTGGT CTGTGAAGCT GTCATTCCAG CCAAGGTTGT CAGAGAAGTA
    TTAAAGACTA CAACAGAGGC TATGATTGAG GTCAACATTA ATAAAAACCT GGTGGGCTCT
    GCCATGGCTG GGAGCATCGG CGGCTACAAC GCCCACGCAG CAAACATCGT AACTGCCATC
    TACATTGCCT GTGGACAGGA TGCAGCACAG AATGTTGGTA GTTCGAACTG TATTACTTTA
    ATGGAAGCAA GCGGTCCCAC GAACGAAGAC CTGTATATCA GCTGCACCAT GCCATCTATA
    GAAATAGGAA CCGTGGGCGG GGGGACCAAC TTGCTCCCTC AGCAGGCCTG CCTGCAGATG
    CTAGGCGTCC AAGGAGCGTG CAGAGACAAC CCCGGGGAAA ACGCGCGGCA GCTGGCCCGA
    ATCGTGTGCG GCACGGTGAT GGCGGGGGAG CTGTCCCTGA TGGCAGCACT GGCAGCAGGC
    CATCTGGTCA GAAGTCACAT GATCCACAAC AGGTCAAAGA TAAATTTGCA AGACCTCCAA
    GGAACTTGCA CTAAGAAGGC AGCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu28003
    Clone ID Related Accession (Same CDS sequence) XM_006074030.1 , XM_006074029.2 , XM_006074029.1 , XM_006074032.1 , XM_006074031.2 , XM_006074030.2 , XM_025296388.1 , XM_006074031.1
    Accession Version XM_006074030.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2667bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product 3-hydroxy-3-methylglutaryl-Coenzyme A reductase isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037555.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006074030.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGTTGTCAA GACTTTTCCG AATGCACGGC CTCTTTGTGG CCTCCCATCC CTGGGAAGTC 
    ATAGTGGGGA CAGTGACGCT GACCATCTGC ATGATGTCCA TGAACATGTT TACTGGCAAC
    AATAAGATCT GTGGTTGGAA TTATGAATGT CCGAAGCTTG AAGAGGATGT TTTGAGCAGC
    GACATCATAA TTCTGACAAT AACACGATGC ATAGCAATAT TGTATATTTA CTTCCAGTTC
    CAGAATTTAC GCCAACTTGG ATCAAAATAT ATTTTGGGTA TTGCTGGGCT CTTCACAATT
    TTCTCCAGTT TCGTATTCAG TACAGTTGTC ATTCATGTCT TAGATAAAGA ATTGACAGGT
    TTGAATGAAG CTTTGCCCTT TTTCCTGCTG CTGGTCGACC TTTCTAGAGC AAGTGCGCTA
    GCCAAGTTCG CCCTCAGTTC TAACTCACAG GATGAAGTGA GGGAGAACAT TGCTCGTGGG
    ATGGCGATTT TAGGTCCCAC CTTCACCCTG GATGCTCTTG TAGAATGTCT CGTGATTGGA
    GTTGGTACCA TGTCAGGGGT GCGTCAGCTC GAAATCATGT GCTGCTTTGG CTGCATGTCG
    GTTCTCGCCA ACTACTTCGT GTTCATGACT TTCTTCCCAG CTTGTGTGTC CTTGGTTCTG
    GAGCTTTCTC GGGAAAGCCG CGAGGGCCGT CCAATCTGGC AGCTCAGCCA TTTTGCCCGA
    GTCTTAGAAG AAGAAGAAAA TAAGCCAAAT CCTGTTACTC AGAGGGTTAA GATGATTATG
    TCTCTAGGCT TGGTTCTTGT TCACGCTCAC AGTCGCTGGA TAGCTGATCC TTCTCCTCAA
    AACAGCACAG CAGACAATTC TAAGGTCTCT TTAGGATTGG ATGAAAATGT GTCCAAGAGA
    ATTGAACCGA GTGTTTCCCT CTGGCAGTTT TATCTCTCTA AAATGATCAG CATGGATATT
    GAACAAGTTA TTACCCTAAG TTTAGCTCTC CTTCTGGCTG TCAAGTATAT CTTCTTTGAA
    CAAGCAGAGA CAGAATCGAC ACTCTCGTTA AAAAATCCTA TCACATCTCC TGTAGTGACC
    CAAAAGAAAG TCACAGACGA TTGTTGTAGA CGTGACCCTG TACTGGTCAG AAATGACCAG
    AAATTCCATG CAATGGAGGA GGAGGCCAGG AAAAACAGAG AAAGAAAAGT TGAGGTTATA
    AAACCCTTAC TGGCAGAAAA TGACACCTCA AACAGAGCTA CATTTGTGGT GGGGAACTCC
    TCCTTACTCA GTACTTCACT GGAACTAGAG ACACAGGAAC CTGAAATTGA ACTTCCCGTG
    GAGCCTCGGC CTAATGAAGA GTGTCTGCAG ATTCTTGAGA ATGCAGAGAA AGGTGCAAAA
    TTCCTTAGTG ATGCTGAGAT CATCCAGTTG GTCAATGCAA AGCATATCCC AGCTTACAAA
    TTGGAAACTC TGATGGAAAC CCATGAGCGA GGTGTATCTA TTCGCCGACA GCTACTTTCC
    AAAAAACTTC CAGAGCCTTC TTCTCTCCAG TATCTACCTT ACAGGGACTA TAATTACTCC
    TTGGTGATGG GAGCTTGCTG TGAGAATGTT ATTGGATATA TGCCCATCCC TGTTGGAGTG
    GCAGGACCTC TGTGCTTGGA TGGAAAAGAA TTTCAGGTTC CAATGGCAAC AACAGAAGGC
    TGTCTCGTGG CCAGCACTAA TAGAGGCTGC AGAGCAATAG GCCTTGGTGG AGGTGCCAGC
    AGCCGGGTCC TTGCAGACGG GATGACTCGT GGCCCAGTGG TGCGTTTTCC CCGTGCTTGT
    GACTCTGCAG AAGTGAAGGC CTGGCTTGAG ACGCCTGAAG GGTTCACAGT GATAAAGGAA
    GCATTCGACA GCACCAGCAG GTTTGCACGT CTACAGAAAC TGCATATGAG TGTGGCCGGA
    CGCAACCTTT ACATCCGTTT TCAGTCCAGG TCAGGTGATG CCATGGGGAT GAACATGATT
    TCAAAGGGTA CAGAGAAAGC ACTTTCCAAA CTTCAGGAGT ATTTCCCCGA AATGCAGATT
    CTGGCAGTTA GTGGGAACTA TTGTACAGAC AAGAAACCTG CGGCCATCAA CTGGATAGAG
    GGACGAGGAA AGTCTGTGGT CTGTGAAGCT GTCATTCCAG CCAAGGTTGT CAGAGAAGTA
    TTAAAGACTA CAACAGAGGC TATGATTGAG GTCAACATTA ATAAAAACCT GGTGGGCTCT
    GCCATGGCTG GGAGCATCGG CGGCTACAAC GCCCACGCAG CAAACATCGT AACTGCCATC
    TACATTGCCT GTGGACAGGA TGCAGCACAG AATGTTGGTA GTTCGAACTG TATTACTTTA
    ATGGAAGCAA GCGGTCCCAC GAACGAAGAC CTGTATATCA GCTGCACCAT GCCATCTATA
    GAAATAGGAA CCGTGGGCGG GGGGACCAAC TTGCTCCCTC AGCAGGCCTG CCTGCAGATG
    CTAGGCGTCC AAGGAGCGTG CAGAGACAAC CCCGGGGAAA ACGCGCGGCA GCTGGCCCGA
    ATCGTGTGCG GCACGGTGAT GGCGGGGGAG CTGTCCCTGA TGGCAGCACT GGCAGCAGGC
    CATCTGGTCA GAAGTCACAT GATCCACAAC AGGTCAAAGA TAAATTTGCA AGACCTCCAA
    GGAACTTGCA CTAAGAAGGC AGCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006074092.1
    CDS153..2819
    Translation

    Target ORF information:

    RefSeq Version XM_006074030.2
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis 3-hydroxy-3-methylglutaryl-CoA reductase (HMGCR), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006074030.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGTTGTCAA GACTTTTCCG AATGCACGGC CTCTTTGTGG CCTCCCATCC CTGGGAAGTC 
    ATAGTGGGGA CAGTGACGCT GACCATCTGC ATGATGTCCA TGAACATGTT TACTGGCAAC
    AATAAGATCT GTGGTTGGAA TTATGAATGT CCGAAGCTTG AAGAGGATGT TTTGAGCAGC
    GACATCATAA TTCTGACAAT AACACGATGC ATAGCAATAT TGTATATTTA CTTCCAGTTC
    CAGAATTTAC GCCAACTTGG ATCAAAATAT ATTTTGGGTA TTGCTGGGCT CTTCACAATT
    TTCTCCAGTT TCGTATTCAG TACAGTTGTC ATTCATGTCT TAGATAAAGA ATTGACAGGT
    TTGAATGAAG CTTTGCCCTT TTTCCTGCTG CTGGTCGACC TTTCTAGAGC AAGTGCGCTA
    GCCAAGTTCG CCCTCAGTTC TAACTCACAG GATGAAGTGA GGGAGAACAT TGCTCGTGGG
    ATGGCGATTT TAGGTCCCAC CTTCACCCTG GATGCTCTTG TAGAATGTCT CGTGATTGGA
    GTTGGTACCA TGTCAGGGGT GCGTCAGCTC GAAATCATGT GCTGCTTTGG CTGCATGTCG
    GTTCTCGCCA ACTACTTCGT GTTCATGACT TTCTTCCCAG CTTGTGTGTC CTTGGTTCTG
    GAGCTTTCTC GGGAAAGCCG CGAGGGCCGT CCAATCTGGC AGCTCAGCCA TTTTGCCCGA
    GTCTTAGAAG AAGAAGAAAA TAAGCCAAAT CCTGTTACTC AGAGGGTTAA GATGATTATG
    TCTCTAGGCT TGGTTCTTGT TCACGCTCAC AGTCGCTGGA TAGCTGATCC TTCTCCTCAA
    AACAGCACAG CAGACAATTC TAAGGTCTCT TTAGGATTGG ATGAAAATGT GTCCAAGAGA
    ATTGAACCGA GTGTTTCCCT CTGGCAGTTT TATCTCTCTA AAATGATCAG CATGGATATT
    GAACAAGTTA TTACCCTAAG TTTAGCTCTC CTTCTGGCTG TCAAGTATAT CTTCTTTGAA
    CAAGCAGAGA CAGAATCGAC ACTCTCGTTA AAAAATCCTA TCACATCTCC TGTAGTGACC
    CAAAAGAAAG TCACAGACGA TTGTTGTAGA CGTGACCCTG TACTGGTCAG AAATGACCAG
    AAATTCCATG CAATGGAGGA GGAGGCCAGG AAAAACAGAG AAAGAAAAGT TGAGGTTATA
    AAACCCTTAC TGGCAGAAAA TGACACCTCA AACAGAGCTA CATTTGTGGT GGGGAACTCC
    TCCTTACTCA GTACTTCACT GGAACTAGAG ACACAGGAAC CTGAAATTGA ACTTCCCGTG
    GAGCCTCGGC CTAATGAAGA GTGTCTGCAG ATTCTTGAGA ATGCAGAGAA AGGTGCAAAA
    TTCCTTAGTG ATGCTGAGAT CATCCAGTTG GTCAATGCAA AGCATATCCC AGCTTACAAA
    TTGGAAACTC TGATGGAAAC CCATGAGCGA GGTGTATCTA TTCGCCGACA GCTACTTTCC
    AAAAAACTTC CAGAGCCTTC TTCTCTCCAG TATCTACCTT ACAGGGACTA TAATTACTCC
    TTGGTGATGG GAGCTTGCTG TGAGAATGTT ATTGGATATA TGCCCATCCC TGTTGGAGTG
    GCAGGACCTC TGTGCTTGGA TGGAAAAGAA TTTCAGGTTC CAATGGCAAC AACAGAAGGC
    TGTCTCGTGG CCAGCACTAA TAGAGGCTGC AGAGCAATAG GCCTTGGTGG AGGTGCCAGC
    AGCCGGGTCC TTGCAGACGG GATGACTCGT GGCCCAGTGG TGCGTTTTCC CCGTGCTTGT
    GACTCTGCAG AAGTGAAGGC CTGGCTTGAG ACGCCTGAAG GGTTCACAGT GATAAAGGAA
    GCATTCGACA GCACCAGCAG GTTTGCACGT CTACAGAAAC TGCATATGAG TGTGGCCGGA
    CGCAACCTTT ACATCCGTTT TCAGTCCAGG TCAGGTGATG CCATGGGGAT GAACATGATT
    TCAAAGGGTA CAGAGAAAGC ACTTTCCAAA CTTCAGGAGT ATTTCCCCGA AATGCAGATT
    CTGGCAGTTA GTGGGAACTA TTGTACAGAC AAGAAACCTG CGGCCATCAA CTGGATAGAG
    GGACGAGGAA AGTCTGTGGT CTGTGAAGCT GTCATTCCAG CCAAGGTTGT CAGAGAAGTA
    TTAAAGACTA CAACAGAGGC TATGATTGAG GTCAACATTA ATAAAAACCT GGTGGGCTCT
    GCCATGGCTG GGAGCATCGG CGGCTACAAC GCCCACGCAG CAAACATCGT AACTGCCATC
    TACATTGCCT GTGGACAGGA TGCAGCACAG AATGTTGGTA GTTCGAACTG TATTACTTTA
    ATGGAAGCAA GCGGTCCCAC GAACGAAGAC CTGTATATCA GCTGCACCAT GCCATCTATA
    GAAATAGGAA CCGTGGGCGG GGGGACCAAC TTGCTCCCTC AGCAGGCCTG CCTGCAGATG
    CTAGGCGTCC AAGGAGCGTG CAGAGACAAC CCCGGGGAAA ACGCGCGGCA GCTGGCCCGA
    ATCGTGTGCG GCACGGTGAT GGCGGGGGAG CTGTCCCTGA TGGCAGCACT GGCAGCAGGC
    CATCTGGTCA GAAGTCACAT GATCCACAAC AGGTCAAAGA TAAATTTGCA AGACCTCCAA
    GGAACTTGCA CTAAGAAGGC AGCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu28003
    Clone ID Related Accession (Same CDS sequence) XM_006074030.1 , XM_006074029.2 , XM_006074029.1 , XM_006074032.1 , XM_006074031.2 , XM_006074030.2 , XM_025296388.1 , XM_006074031.1
    Accession Version XM_025296388.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2667bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product 3-hydroxy-3-methylglutaryl-Coenzyme A reductase isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037555.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGTTGTCAA GACTTTTCCG AATGCACGGC CTCTTTGTGG CCTCCCATCC CTGGGAAGTC 
    ATAGTGGGGA CAGTGACGCT GACCATCTGC ATGATGTCCA TGAACATGTT TACTGGCAAC
    AATAAGATCT GTGGTTGGAA TTATGAATGT CCGAAGCTTG AAGAGGATGT TTTGAGCAGC
    GACATCATAA TTCTGACAAT AACACGATGC ATAGCAATAT TGTATATTTA CTTCCAGTTC
    CAGAATTTAC GCCAACTTGG ATCAAAATAT ATTTTGGGTA TTGCTGGGCT CTTCACAATT
    TTCTCCAGTT TCGTATTCAG TACAGTTGTC ATTCATGTCT TAGATAAAGA ATTGACAGGT
    TTGAATGAAG CTTTGCCCTT TTTCCTGCTG CTGGTCGACC TTTCTAGAGC AAGTGCGCTA
    GCCAAGTTCG CCCTCAGTTC TAACTCACAG GATGAAGTGA GGGAGAACAT TGCTCGTGGG
    ATGGCGATTT TAGGTCCCAC CTTCACCCTG GATGCTCTTG TAGAATGTCT CGTGATTGGA
    GTTGGTACCA TGTCAGGGGT GCGTCAGCTC GAAATCATGT GCTGCTTTGG CTGCATGTCG
    GTTCTCGCCA ACTACTTCGT GTTCATGACT TTCTTCCCAG CTTGTGTGTC CTTGGTTCTG
    GAGCTTTCTC GGGAAAGCCG CGAGGGCCGT CCAATCTGGC AGCTCAGCCA TTTTGCCCGA
    GTCTTAGAAG AAGAAGAAAA TAAGCCAAAT CCTGTTACTC AGAGGGTTAA GATGATTATG
    TCTCTAGGCT TGGTTCTTGT TCACGCTCAC AGTCGCTGGA TAGCTGATCC TTCTCCTCAA
    AACAGCACAG CAGACAATTC TAAGGTCTCT TTAGGATTGG ATGAAAATGT GTCCAAGAGA
    ATTGAACCGA GTGTTTCCCT CTGGCAGTTT TATCTCTCTA AAATGATCAG CATGGATATT
    GAACAAGTTA TTACCCTAAG TTTAGCTCTC CTTCTGGCTG TCAAGTATAT CTTCTTTGAA
    CAAGCAGAGA CAGAATCGAC ACTCTCGTTA AAAAATCCTA TCACATCTCC TGTAGTGACC
    CAAAAGAAAG TCACAGACGA TTGTTGTAGA CGTGACCCTG TACTGGTCAG AAATGACCAG
    AAATTCCATG CAATGGAGGA GGAGGCCAGG AAAAACAGAG AAAGAAAAGT TGAGGTTATA
    AAACCCTTAC TGGCAGAAAA TGACACCTCA AACAGAGCTA CATTTGTGGT GGGGAACTCC
    TCCTTACTCA GTACTTCACT GGAACTAGAG ACACAGGAAC CTGAAATTGA ACTTCCCGTG
    GAGCCTCGGC CTAATGAAGA GTGTCTGCAG ATTCTTGAGA ATGCAGAGAA AGGTGCAAAA
    TTCCTTAGTG ATGCTGAGAT CATCCAGTTG GTCAATGCAA AGCATATCCC AGCTTACAAA
    TTGGAAACTC TGATGGAAAC CCATGAGCGA GGTGTATCTA TTCGCCGACA GCTACTTTCC
    AAAAAACTTC CAGAGCCTTC TTCTCTCCAG TATCTACCTT ACAGGGACTA TAATTACTCC
    TTGGTGATGG GAGCTTGCTG TGAGAATGTT ATTGGATATA TGCCCATCCC TGTTGGAGTG
    GCAGGACCTC TGTGCTTGGA TGGAAAAGAA TTTCAGGTTC CAATGGCAAC AACAGAAGGC
    TGTCTCGTGG CCAGCACTAA TAGAGGCTGC AGAGCAATAG GCCTTGGTGG AGGTGCCAGC
    AGCCGGGTCC TTGCAGACGG GATGACTCGT GGCCCAGTGG TGCGTTTTCC CCGTGCTTGT
    GACTCTGCAG AAGTGAAGGC CTGGCTTGAG ACGCCTGAAG GGTTCACAGT GATAAAGGAA
    GCATTCGACA GCACCAGCAG GTTTGCACGT CTACAGAAAC TGCATATGAG TGTGGCCGGA
    CGCAACCTTT ACATCCGTTT TCAGTCCAGG TCAGGTGATG CCATGGGGAT GAACATGATT
    TCAAAGGGTA CAGAGAAAGC ACTTTCCAAA CTTCAGGAGT ATTTCCCCGA AATGCAGATT
    CTGGCAGTTA GTGGGAACTA TTGTACAGAC AAGAAACCTG CGGCCATCAA CTGGATAGAG
    GGACGAGGAA AGTCTGTGGT CTGTGAAGCT GTCATTCCAG CCAAGGTTGT CAGAGAAGTA
    TTAAAGACTA CAACAGAGGC TATGATTGAG GTCAACATTA ATAAAAACCT GGTGGGCTCT
    GCCATGGCTG GGAGCATCGG CGGCTACAAC GCCCACGCAG CAAACATCGT AACTGCCATC
    TACATTGCCT GTGGACAGGA TGCAGCACAG AATGTTGGTA GTTCGAACTG TATTACTTTA
    ATGGAAGCAA GCGGTCCCAC GAACGAAGAC CTGTATATCA GCTGCACCAT GCCATCTATA
    GAAATAGGAA CCGTGGGCGG GGGGACCAAC TTGCTCCCTC AGCAGGCCTG CCTGCAGATG
    CTAGGCGTCC AAGGAGCGTG CAGAGACAAC CCCGGGGAAA ACGCGCGGCA GCTGGCCCGA
    ATCGTGTGCG GCACGGTGAT GGCGGGGGAG CTGTCCCTGA TGGCAGCACT GGCAGCAGGC
    CATCTGGTCA GAAGTCACAT GATCCACAAC AGGTCAAAGA TAAATTTGCA AGACCTCCAA
    GGAACTTGCA CTAAGAAGGC AGCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025152173.1
    CDS223..2889
    Translation

    Target ORF information:

    RefSeq Version XM_025296388.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis 3-hydroxy-3-methylglutaryl-CoA reductase (HMGCR), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025296388.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGTTGTCAA GACTTTTCCG AATGCACGGC CTCTTTGTGG CCTCCCATCC CTGGGAAGTC 
    ATAGTGGGGA CAGTGACGCT GACCATCTGC ATGATGTCCA TGAACATGTT TACTGGCAAC
    AATAAGATCT GTGGTTGGAA TTATGAATGT CCGAAGCTTG AAGAGGATGT TTTGAGCAGC
    GACATCATAA TTCTGACAAT AACACGATGC ATAGCAATAT TGTATATTTA CTTCCAGTTC
    CAGAATTTAC GCCAACTTGG ATCAAAATAT ATTTTGGGTA TTGCTGGGCT CTTCACAATT
    TTCTCCAGTT TCGTATTCAG TACAGTTGTC ATTCATGTCT TAGATAAAGA ATTGACAGGT
    TTGAATGAAG CTTTGCCCTT TTTCCTGCTG CTGGTCGACC TTTCTAGAGC AAGTGCGCTA
    GCCAAGTTCG CCCTCAGTTC TAACTCACAG GATGAAGTGA GGGAGAACAT TGCTCGTGGG
    ATGGCGATTT TAGGTCCCAC CTTCACCCTG GATGCTCTTG TAGAATGTCT CGTGATTGGA
    GTTGGTACCA TGTCAGGGGT GCGTCAGCTC GAAATCATGT GCTGCTTTGG CTGCATGTCG
    GTTCTCGCCA ACTACTTCGT GTTCATGACT TTCTTCCCAG CTTGTGTGTC CTTGGTTCTG
    GAGCTTTCTC GGGAAAGCCG CGAGGGCCGT CCAATCTGGC AGCTCAGCCA TTTTGCCCGA
    GTCTTAGAAG AAGAAGAAAA TAAGCCAAAT CCTGTTACTC AGAGGGTTAA GATGATTATG
    TCTCTAGGCT TGGTTCTTGT TCACGCTCAC AGTCGCTGGA TAGCTGATCC TTCTCCTCAA
    AACAGCACAG CAGACAATTC TAAGGTCTCT TTAGGATTGG ATGAAAATGT GTCCAAGAGA
    ATTGAACCGA GTGTTTCCCT CTGGCAGTTT TATCTCTCTA AAATGATCAG CATGGATATT
    GAACAAGTTA TTACCCTAAG TTTAGCTCTC CTTCTGGCTG TCAAGTATAT CTTCTTTGAA
    CAAGCAGAGA CAGAATCGAC ACTCTCGTTA AAAAATCCTA TCACATCTCC TGTAGTGACC
    CAAAAGAAAG TCACAGACGA TTGTTGTAGA CGTGACCCTG TACTGGTCAG AAATGACCAG
    AAATTCCATG CAATGGAGGA GGAGGCCAGG AAAAACAGAG AAAGAAAAGT TGAGGTTATA
    AAACCCTTAC TGGCAGAAAA TGACACCTCA AACAGAGCTA CATTTGTGGT GGGGAACTCC
    TCCTTACTCA GTACTTCACT GGAACTAGAG ACACAGGAAC CTGAAATTGA ACTTCCCGTG
    GAGCCTCGGC CTAATGAAGA GTGTCTGCAG ATTCTTGAGA ATGCAGAGAA AGGTGCAAAA
    TTCCTTAGTG ATGCTGAGAT CATCCAGTTG GTCAATGCAA AGCATATCCC AGCTTACAAA
    TTGGAAACTC TGATGGAAAC CCATGAGCGA GGTGTATCTA TTCGCCGACA GCTACTTTCC
    AAAAAACTTC CAGAGCCTTC TTCTCTCCAG TATCTACCTT ACAGGGACTA TAATTACTCC
    TTGGTGATGG GAGCTTGCTG TGAGAATGTT ATTGGATATA TGCCCATCCC TGTTGGAGTG
    GCAGGACCTC TGTGCTTGGA TGGAAAAGAA TTTCAGGTTC CAATGGCAAC AACAGAAGGC
    TGTCTCGTGG CCAGCACTAA TAGAGGCTGC AGAGCAATAG GCCTTGGTGG AGGTGCCAGC
    AGCCGGGTCC TTGCAGACGG GATGACTCGT GGCCCAGTGG TGCGTTTTCC CCGTGCTTGT
    GACTCTGCAG AAGTGAAGGC CTGGCTTGAG ACGCCTGAAG GGTTCACAGT GATAAAGGAA
    GCATTCGACA GCACCAGCAG GTTTGCACGT CTACAGAAAC TGCATATGAG TGTGGCCGGA
    CGCAACCTTT ACATCCGTTT TCAGTCCAGG TCAGGTGATG CCATGGGGAT GAACATGATT
    TCAAAGGGTA CAGAGAAAGC ACTTTCCAAA CTTCAGGAGT ATTTCCCCGA AATGCAGATT
    CTGGCAGTTA GTGGGAACTA TTGTACAGAC AAGAAACCTG CGGCCATCAA CTGGATAGAG
    GGACGAGGAA AGTCTGTGGT CTGTGAAGCT GTCATTCCAG CCAAGGTTGT CAGAGAAGTA
    TTAAAGACTA CAACAGAGGC TATGATTGAG GTCAACATTA ATAAAAACCT GGTGGGCTCT
    GCCATGGCTG GGAGCATCGG CGGCTACAAC GCCCACGCAG CAAACATCGT AACTGCCATC
    TACATTGCCT GTGGACAGGA TGCAGCACAG AATGTTGGTA GTTCGAACTG TATTACTTTA
    ATGGAAGCAA GCGGTCCCAC GAACGAAGAC CTGTATATCA GCTGCACCAT GCCATCTATA
    GAAATAGGAA CCGTGGGCGG GGGGACCAAC TTGCTCCCTC AGCAGGCCTG CCTGCAGATG
    CTAGGCGTCC AAGGAGCGTG CAGAGACAAC CCCGGGGAAA ACGCGCGGCA GCTGGCCCGA
    ATCGTGTGCG GCACGGTGAT GGCGGGGGAG CTGTCCCTGA TGGCAGCACT GGCAGCAGGC
    CATCTGGTCA GAAGTCACAT GATCCACAAC AGGTCAAAGA TAAATTTGCA AGACCTCCAA
    GGAACTTGCA CTAAGAAGGC AGCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu28003
    Clone ID Related Accession (Same CDS sequence) XM_006074030.1 , XM_006074029.2 , XM_006074029.1 , XM_006074032.1 , XM_006074031.2 , XM_006074030.2 , XM_025296388.1 , XM_006074031.1
    Accession Version XM_006074031.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2667bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2014-03-11
    Organism Bubalus bubalis(water buffalo)
    Product 3-hydroxy-3-methylglutaryl-coenzyme A reductase isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785570.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGTTGTCAA GACTTTTCCG AATGCACGGC CTCTTTGTGG CCTCCCATCC CTGGGAAGTC 
    ATAGTGGGGA CAGTGACGCT GACCATCTGC ATGATGTCCA TGAACATGTT TACTGGCAAC
    AATAAGATCT GTGGTTGGAA TTATGAATGT CCGAAGCTTG AAGAGGATGT TTTGAGCAGC
    GACATCATAA TTCTGACAAT AACACGATGC ATAGCAATAT TGTATATTTA CTTCCAGTTC
    CAGAATTTAC GCCAACTTGG ATCAAAATAT ATTTTGGGTA TTGCTGGGCT CTTCACAATT
    TTCTCCAGTT TCGTATTCAG TACAGTTGTC ATTCATGTCT TAGATAAAGA ATTGACAGGT
    TTGAATGAAG CTTTGCCCTT TTTCCTGCTG CTGGTCGACC TTTCTAGAGC AAGTGCGCTA
    GCCAAGTTCG CCCTCAGTTC TAACTCACAG GATGAAGTGA GGGAGAACAT TGCTCGTGGG
    ATGGCGATTT TAGGTCCCAC CTTCACCCTG GATGCTCTTG TAGAATGTCT CGTGATTGGA
    GTTGGTACCA TGTCAGGGGT GCGTCAGCTC GAAATCATGT GCTGCTTTGG CTGCATGTCG
    GTTCTCGCCA ACTACTTCGT GTTCATGACT TTCTTCCCAG CTTGTGTGTC CTTGGTTCTG
    GAGCTTTCTC GGGAAAGCCG CGAGGGCCGT CCAATCTGGC AGCTCAGCCA TTTTGCCCGA
    GTCTTAGAAG AAGAAGAAAA TAAGCCAAAT CCTGTTACTC AGAGGGTTAA GATGATTATG
    TCTCTAGGCT TGGTTCTTGT TCACGCTCAC AGTCGCTGGA TAGCTGATCC TTCTCCTCAA
    AACAGCACAG CAGACAATTC TAAGGTCTCT TTAGGATTGG ATGAAAATGT GTCCAAGAGA
    ATTGAACCGA GTGTTTCCCT CTGGCAGTTT TATCTCTCTA AAATGATCAG CATGGATATT
    GAACAAGTTA TTACCCTAAG TTTAGCTCTC CTTCTGGCTG TCAAGTATAT CTTCTTTGAA
    CAAGCAGAGA CAGAATCGAC ACTCTCGTTA AAAAATCCTA TCACATCTCC TGTAGTGACC
    CAAAAGAAAG TCACAGACGA TTGTTGTAGA CGTGACCCTG TACTGGTCAG AAATGACCAG
    AAATTCCATG CAATGGAGGA GGAGGCCAGG AAAAACAGAG AAAGAAAAGT TGAGGTTATA
    AAACCCTTAC TGGCAGAAAA TGACACCTCA AACAGAGCTA CATTTGTGGT GGGGAACTCC
    TCCTTACTCA GTACTTCACT GGAACTAGAG ACACAGGAAC CTGAAATTGA ACTTCCCGTG
    GAGCCTCGGC CTAATGAAGA GTGTCTGCAG ATTCTTGAGA ATGCAGAGAA AGGTGCAAAA
    TTCCTTAGTG ATGCTGAGAT CATCCAGTTG GTCAATGCAA AGCATATCCC AGCTTACAAA
    TTGGAAACTC TGATGGAAAC CCATGAGCGA GGTGTATCTA TTCGCCGACA GCTACTTTCC
    AAAAAACTTC CAGAGCCTTC TTCTCTCCAG TATCTACCTT ACAGGGACTA TAATTACTCC
    TTGGTGATGG GAGCTTGCTG TGAGAATGTT ATTGGATATA TGCCCATCCC TGTTGGAGTG
    GCAGGACCTC TGTGCTTGGA TGGAAAAGAA TTTCAGGTTC CAATGGCAAC AACAGAAGGC
    TGTCTCGTGG CCAGCACTAA TAGAGGCTGC AGAGCAATAG GCCTTGGTGG AGGTGCCAGC
    AGCCGGGTCC TTGCAGACGG GATGACTCGT GGCCCAGTGG TGCGTTTTCC CCGTGCTTGT
    GACTCTGCAG AAGTGAAGGC CTGGCTTGAG ACGCCTGAAG GGTTCACAGT GATAAAGGAA
    GCATTCGACA GCACCAGCAG GTTTGCACGT CTACAGAAAC TGCATATGAG TGTGGCCGGA
    CGCAACCTTT ACATCCGTTT TCAGTCCAGG TCAGGTGATG CCATGGGGAT GAACATGATT
    TCAAAGGGTA CAGAGAAAGC ACTTTCCAAA CTTCAGGAGT ATTTCCCCGA AATGCAGATT
    CTGGCAGTTA GTGGGAACTA TTGTACAGAC AAGAAACCTG CGGCCATCAA CTGGATAGAG
    GGACGAGGAA AGTCTGTGGT CTGTGAAGCT GTCATTCCAG CCAAGGTTGT CAGAGAAGTA
    TTAAAGACTA CAACAGAGGC TATGATTGAG GTCAACATTA ATAAAAACCT GGTGGGCTCT
    GCCATGGCTG GGAGCATCGG CGGCTACAAC GCCCACGCAG CAAACATCGT AACTGCCATC
    TACATTGCCT GTGGACAGGA TGCAGCACAG AATGTTGGTA GTTCGAACTG TATTACTTTA
    ATGGAAGCAA GCGGTCCCAC GAACGAAGAC CTGTATATCA GCTGCACCAT GCCATCTATA
    GAAATAGGAA CCGTGGGCGG GGGGACCAAC TTGCTCCCTC AGCAGGCCTG CCTGCAGATG
    CTAGGCGTCC AAGGAGCGTG CAGAGACAAC CCCGGGGAAA ACGCGCGGCA GCTGGCCCGA
    ATCGTGTGCG GCACGGTGAT GGCGGGGGAG CTGTCCCTGA TGGCAGCACT GGCAGCAGGC
    CATCTGGTCA GAAGTCACAT GATCCACAAC AGGTCAAAGA TAAATTTGCA AGACCTCCAA
    GGAACTTGCA CTAAGAAGGC AGCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006074093.1
    CDS115..2781
    Translation

    Target ORF information:

    RefSeq Version XM_006074031.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis 3-hydroxy-3-methylglutaryl-CoA reductase (HMGCR), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006074031.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGTTGTCAA GACTTTTCCG AATGCACGGC CTCTTTGTGG CCTCCCATCC CTGGGAAGTC 
    ATAGTGGGGA CAGTGACGCT GACCATCTGC ATGATGTCCA TGAACATGTT TACTGGCAAC
    AATAAGATCT GTGGTTGGAA TTATGAATGT CCGAAGCTTG AAGAGGATGT TTTGAGCAGC
    GACATCATAA TTCTGACAAT AACACGATGC ATAGCAATAT TGTATATTTA CTTCCAGTTC
    CAGAATTTAC GCCAACTTGG ATCAAAATAT ATTTTGGGTA TTGCTGGGCT CTTCACAATT
    TTCTCCAGTT TCGTATTCAG TACAGTTGTC ATTCATGTCT TAGATAAAGA ATTGACAGGT
    TTGAATGAAG CTTTGCCCTT TTTCCTGCTG CTGGTCGACC TTTCTAGAGC AAGTGCGCTA
    GCCAAGTTCG CCCTCAGTTC TAACTCACAG GATGAAGTGA GGGAGAACAT TGCTCGTGGG
    ATGGCGATTT TAGGTCCCAC CTTCACCCTG GATGCTCTTG TAGAATGTCT CGTGATTGGA
    GTTGGTACCA TGTCAGGGGT GCGTCAGCTC GAAATCATGT GCTGCTTTGG CTGCATGTCG
    GTTCTCGCCA ACTACTTCGT GTTCATGACT TTCTTCCCAG CTTGTGTGTC CTTGGTTCTG
    GAGCTTTCTC GGGAAAGCCG CGAGGGCCGT CCAATCTGGC AGCTCAGCCA TTTTGCCCGA
    GTCTTAGAAG AAGAAGAAAA TAAGCCAAAT CCTGTTACTC AGAGGGTTAA GATGATTATG
    TCTCTAGGCT TGGTTCTTGT TCACGCTCAC AGTCGCTGGA TAGCTGATCC TTCTCCTCAA
    AACAGCACAG CAGACAATTC TAAGGTCTCT TTAGGATTGG ATGAAAATGT GTCCAAGAGA
    ATTGAACCGA GTGTTTCCCT CTGGCAGTTT TATCTCTCTA AAATGATCAG CATGGATATT
    GAACAAGTTA TTACCCTAAG TTTAGCTCTC CTTCTGGCTG TCAAGTATAT CTTCTTTGAA
    CAAGCAGAGA CAGAATCGAC ACTCTCGTTA AAAAATCCTA TCACATCTCC TGTAGTGACC
    CAAAAGAAAG TCACAGACGA TTGTTGTAGA CGTGACCCTG TACTGGTCAG AAATGACCAG
    AAATTCCATG CAATGGAGGA GGAGGCCAGG AAAAACAGAG AAAGAAAAGT TGAGGTTATA
    AAACCCTTAC TGGCAGAAAA TGACACCTCA AACAGAGCTA CATTTGTGGT GGGGAACTCC
    TCCTTACTCA GTACTTCACT GGAACTAGAG ACACAGGAAC CTGAAATTGA ACTTCCCGTG
    GAGCCTCGGC CTAATGAAGA GTGTCTGCAG ATTCTTGAGA ATGCAGAGAA AGGTGCAAAA
    TTCCTTAGTG ATGCTGAGAT CATCCAGTTG GTCAATGCAA AGCATATCCC AGCTTACAAA
    TTGGAAACTC TGATGGAAAC CCATGAGCGA GGTGTATCTA TTCGCCGACA GCTACTTTCC
    AAAAAACTTC CAGAGCCTTC TTCTCTCCAG TATCTACCTT ACAGGGACTA TAATTACTCC
    TTGGTGATGG GAGCTTGCTG TGAGAATGTT ATTGGATATA TGCCCATCCC TGTTGGAGTG
    GCAGGACCTC TGTGCTTGGA TGGAAAAGAA TTTCAGGTTC CAATGGCAAC AACAGAAGGC
    TGTCTCGTGG CCAGCACTAA TAGAGGCTGC AGAGCAATAG GCCTTGGTGG AGGTGCCAGC
    AGCCGGGTCC TTGCAGACGG GATGACTCGT GGCCCAGTGG TGCGTTTTCC CCGTGCTTGT
    GACTCTGCAG AAGTGAAGGC CTGGCTTGAG ACGCCTGAAG GGTTCACAGT GATAAAGGAA
    GCATTCGACA GCACCAGCAG GTTTGCACGT CTACAGAAAC TGCATATGAG TGTGGCCGGA
    CGCAACCTTT ACATCCGTTT TCAGTCCAGG TCAGGTGATG CCATGGGGAT GAACATGATT
    TCAAAGGGTA CAGAGAAAGC ACTTTCCAAA CTTCAGGAGT ATTTCCCCGA AATGCAGATT
    CTGGCAGTTA GTGGGAACTA TTGTACAGAC AAGAAACCTG CGGCCATCAA CTGGATAGAG
    GGACGAGGAA AGTCTGTGGT CTGTGAAGCT GTCATTCCAG CCAAGGTTGT CAGAGAAGTA
    TTAAAGACTA CAACAGAGGC TATGATTGAG GTCAACATTA ATAAAAACCT GGTGGGCTCT
    GCCATGGCTG GGAGCATCGG CGGCTACAAC GCCCACGCAG CAAACATCGT AACTGCCATC
    TACATTGCCT GTGGACAGGA TGCAGCACAG AATGTTGGTA GTTCGAACTG TATTACTTTA
    ATGGAAGCAA GCGGTCCCAC GAACGAAGAC CTGTATATCA GCTGCACCAT GCCATCTATA
    GAAATAGGAA CCGTGGGCGG GGGGACCAAC TTGCTCCCTC AGCAGGCCTG CCTGCAGATG
    CTAGGCGTCC AAGGAGCGTG CAGAGACAAC CCCGGGGAAA ACGCGCGGCA GCTGGCCCGA
    ATCGTGTGCG GCACGGTGAT GGCGGGGGAG CTGTCCCTGA TGGCAGCACT GGCAGCAGGC
    CATCTGGTCA GAAGTCACAT GATCCACAAC AGGTCAAAGA TAAATTTGCA AGACCTCCAA
    GGAACTTGCA CTAAGAAGGC AGCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu45465
    Clone ID Related Accession (Same CDS sequence) XM_025296389.1
    Accession Version XM_025296389.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2508bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product 3-hydroxy-3-methylglutaryl-Coenzyme A reductase isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037555.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGTTGTCAA GACTTTTCCG AATGCACGGC CTCTTTGTGG CCTCCCATCC CTGGGAAGTC 
    ATAGTGGGGA CAGTGACGCT GACCATCTGC ATGATGTCCA TGAACATGTT TACTGGCAAC
    AATAAGATCT GTGGTTGGAA TTATGAATGT CCGAAGCTTG AAGAGGATGT TTTGAGCAGC
    GACATCATAA TTCTGACAAT AACACGATGC ATAGCAATAT TGTATATTTA CTTCCAGTTC
    CAGAATTTAC GCCAACTTGG ATCAAAATAT ATTTTGGGTA TTGCTGGGCT CTTCACAATT
    TTCTCCAGTT TCGTATTCAG TACAGTTGTC ATTCATGTCT TAGATAAAGA ATTGACAGGT
    TTGAATGAAG CTTTGCCCTT TTTCCTGCTG CTGGTCGACC TTTCTAGAGC AAGTGCGCTA
    GCCAAGTTCG CCCTCAGTTC TAACTCACAG GATGAAGTGA GGGAGAACAT TGCTCGTGGG
    ATGGCGATTT TAGGTCCCAC CTTCACCCTG GATGCTCTTG TAGAATGTCT CGTGATTGGA
    GTTGGTACCA TGTCAGGGGT GCGTCAGCTC GAAATCATGT GCTGCTTTGG CTGCATGTCG
    GTTCTCGCCA ACTACTTCGT GTTCATGACT TTCTTCCCAG CTTGTGTGTC CTTGGTTCTG
    GAGCTTTCTC GGGAAAGCCG CGAGGGCCGT CCAATCTGGC AGCTCAGCCA TTTTGCCCGA
    GTCTTAGAAG AAGAAGAAAA TAAGCCAAAT CCTGTTACTC AGAGGGTTAA GATGATTATG
    TCTCTAGGCT TGGTTCTTGT TCACGCTCAC AGTCGCTGGA TAGCTGATCC TTCTCCTCAA
    AACAGCACAG CAGACAATTC TAAGGTCTCT TTAGGATTGG ATGAAAATGT GTCCAAGAGA
    ATTGAACCGA GTGTTTCCCT CTGGCAGTTT TATCTCTCTA AAATGATCAG CATGGATATT
    GAACAAGTTA TTACCCTAAG TTTAGCTCTC CTTCTGGCTG TCAAGTATAT CTTCTTTGAA
    CAAGCAGAGA CAGAATCGAC ACTCTCGTTA AAAAATCCTA TCACATCTCC TGTAGTGACC
    CAAAAGAAAG TCACAGACGA TTGTTGTAGA CGTGACCCTG TACTGGTCAG AAATGACCAG
    AAATTCCATG CAATGGAGGA GGAGGCCAGG AAAAACAGAG AAAGAAAAGT TGAGGTTATA
    AAACCCTTAC TGGCAGAAAA TGACACCTCA AACAGAGCTA CATTTGTGGT GGGGAACTCC
    TCCTTACTCA GTACTTCACT GGAACTAGAG ACACAGGAAC CTGAAATTGA ACTTCCCGTG
    GAGCCTCGGC CTAATGAAGA GTGTCTGCAG ATTCTTGAGA ATGCAGAGAA AGGTGCAAAA
    TTCCTTAGTG ATGCTGAGAT CATCCAGTTG GTCAATGCAA AGCATATCCC AGCTTACAAA
    TTGGAAACTC TGATGGAAAC CCATGAGCGA GGTGTATCTA TTCGCCGACA GCTACTTTCC
    AAAAAACTTC CAGAGCCTTC TTCTCTCCAG TATCTACCTT ACAGGGACTA TAATTACTCC
    TTGCTTGGTG GAGGTGCCAG CAGCCGGGTC CTTGCAGACG GGATGACTCG TGGCCCAGTG
    GTGCGTTTTC CCCGTGCTTG TGACTCTGCA GAAGTGAAGG CCTGGCTTGA GACGCCTGAA
    GGGTTCACAG TGATAAAGGA AGCATTCGAC AGCACCAGCA GGTTTGCACG TCTACAGAAA
    CTGCATATGA GTGTGGCCGG ACGCAACCTT TACATCCGTT TTCAGTCCAG GTCAGGTGAT
    GCCATGGGGA TGAACATGAT TTCAAAGGGT ACAGAGAAAG CACTTTCCAA ACTTCAGGAG
    TATTTCCCCG AAATGCAGAT TCTGGCAGTT AGTGGGAACT ATTGTACAGA CAAGAAACCT
    GCGGCCATCA ACTGGATAGA GGGACGAGGA AAGTCTGTGG TCTGTGAAGC TGTCATTCCA
    GCCAAGGTTG TCAGAGAAGT ATTAAAGACT ACAACAGAGG CTATGATTGA GGTCAACATT
    AATAAAAACC TGGTGGGCTC TGCCATGGCT GGGAGCATCG GCGGCTACAA CGCCCACGCA
    GCAAACATCG TAACTGCCAT CTACATTGCC TGTGGACAGG ATGCAGCACA GAATGTTGGT
    AGTTCGAACT GTATTACTTT AATGGAAGCA AGCGGTCCCA CGAACGAAGA CCTGTATATC
    AGCTGCACCA TGCCATCTAT AGAAATAGGA ACCGTGGGCG GGGGGACCAA CTTGCTCCCT
    CAGCAGGCCT GCCTGCAGAT GCTAGGCGTC CAAGGAGCGT GCAGAGACAA CCCCGGGGAA
    AACGCGCGGC AGCTGGCCCG AATCGTGTGC GGCACGGTGA TGGCGGGGGA GCTGTCCCTG
    ATGGCAGCAC TGGCAGCAGG CCATCTGGTC AGAAGTCACA TGATCCACAA CAGGTCAAAG
    ATAAATTTGC AAGACCTCCA AGGAACTTGC ACTAAGAAGG CAGCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025152174.1
    CDS157..2664
    Translation

    Target ORF information:

    RefSeq Version XM_025296389.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis 3-hydroxy-3-methylglutaryl-CoA reductase (HMGCR), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025296389.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGTTGTCAA GACTTTTCCG AATGCACGGC CTCTTTGTGG CCTCCCATCC CTGGGAAGTC 
    ATAGTGGGGA CAGTGACGCT GACCATCTGC ATGATGTCCA TGAACATGTT TACTGGCAAC
    AATAAGATCT GTGGTTGGAA TTATGAATGT CCGAAGCTTG AAGAGGATGT TTTGAGCAGC
    GACATCATAA TTCTGACAAT AACACGATGC ATAGCAATAT TGTATATTTA CTTCCAGTTC
    CAGAATTTAC GCCAACTTGG ATCAAAATAT ATTTTGGGTA TTGCTGGGCT CTTCACAATT
    TTCTCCAGTT TCGTATTCAG TACAGTTGTC ATTCATGTCT TAGATAAAGA ATTGACAGGT
    TTGAATGAAG CTTTGCCCTT TTTCCTGCTG CTGGTCGACC TTTCTAGAGC AAGTGCGCTA
    GCCAAGTTCG CCCTCAGTTC TAACTCACAG GATGAAGTGA GGGAGAACAT TGCTCGTGGG
    ATGGCGATTT TAGGTCCCAC CTTCACCCTG GATGCTCTTG TAGAATGTCT CGTGATTGGA
    GTTGGTACCA TGTCAGGGGT GCGTCAGCTC GAAATCATGT GCTGCTTTGG CTGCATGTCG
    GTTCTCGCCA ACTACTTCGT GTTCATGACT TTCTTCCCAG CTTGTGTGTC CTTGGTTCTG
    GAGCTTTCTC GGGAAAGCCG CGAGGGCCGT CCAATCTGGC AGCTCAGCCA TTTTGCCCGA
    GTCTTAGAAG AAGAAGAAAA TAAGCCAAAT CCTGTTACTC AGAGGGTTAA GATGATTATG
    TCTCTAGGCT TGGTTCTTGT TCACGCTCAC AGTCGCTGGA TAGCTGATCC TTCTCCTCAA
    AACAGCACAG CAGACAATTC TAAGGTCTCT TTAGGATTGG ATGAAAATGT GTCCAAGAGA
    ATTGAACCGA GTGTTTCCCT CTGGCAGTTT TATCTCTCTA AAATGATCAG CATGGATATT
    GAACAAGTTA TTACCCTAAG TTTAGCTCTC CTTCTGGCTG TCAAGTATAT CTTCTTTGAA
    CAAGCAGAGA CAGAATCGAC ACTCTCGTTA AAAAATCCTA TCACATCTCC TGTAGTGACC
    CAAAAGAAAG TCACAGACGA TTGTTGTAGA CGTGACCCTG TACTGGTCAG AAATGACCAG
    AAATTCCATG CAATGGAGGA GGAGGCCAGG AAAAACAGAG AAAGAAAAGT TGAGGTTATA
    AAACCCTTAC TGGCAGAAAA TGACACCTCA AACAGAGCTA CATTTGTGGT GGGGAACTCC
    TCCTTACTCA GTACTTCACT GGAACTAGAG ACACAGGAAC CTGAAATTGA ACTTCCCGTG
    GAGCCTCGGC CTAATGAAGA GTGTCTGCAG ATTCTTGAGA ATGCAGAGAA AGGTGCAAAA
    TTCCTTAGTG ATGCTGAGAT CATCCAGTTG GTCAATGCAA AGCATATCCC AGCTTACAAA
    TTGGAAACTC TGATGGAAAC CCATGAGCGA GGTGTATCTA TTCGCCGACA GCTACTTTCC
    AAAAAACTTC CAGAGCCTTC TTCTCTCCAG TATCTACCTT ACAGGGACTA TAATTACTCC
    TTGCTTGGTG GAGGTGCCAG CAGCCGGGTC CTTGCAGACG GGATGACTCG TGGCCCAGTG
    GTGCGTTTTC CCCGTGCTTG TGACTCTGCA GAAGTGAAGG CCTGGCTTGA GACGCCTGAA
    GGGTTCACAG TGATAAAGGA AGCATTCGAC AGCACCAGCA GGTTTGCACG TCTACAGAAA
    CTGCATATGA GTGTGGCCGG ACGCAACCTT TACATCCGTT TTCAGTCCAG GTCAGGTGAT
    GCCATGGGGA TGAACATGAT TTCAAAGGGT ACAGAGAAAG CACTTTCCAA ACTTCAGGAG
    TATTTCCCCG AAATGCAGAT TCTGGCAGTT AGTGGGAACT ATTGTACAGA CAAGAAACCT
    GCGGCCATCA ACTGGATAGA GGGACGAGGA AAGTCTGTGG TCTGTGAAGC TGTCATTCCA
    GCCAAGGTTG TCAGAGAAGT ATTAAAGACT ACAACAGAGG CTATGATTGA GGTCAACATT
    AATAAAAACC TGGTGGGCTC TGCCATGGCT GGGAGCATCG GCGGCTACAA CGCCCACGCA
    GCAAACATCG TAACTGCCAT CTACATTGCC TGTGGACAGG ATGCAGCACA GAATGTTGGT
    AGTTCGAACT GTATTACTTT AATGGAAGCA AGCGGTCCCA CGAACGAAGA CCTGTATATC
    AGCTGCACCA TGCCATCTAT AGAAATAGGA ACCGTGGGCG GGGGGACCAA CTTGCTCCCT
    CAGCAGGCCT GCCTGCAGAT GCTAGGCGTC CAAGGAGCGT GCAGAGACAA CCCCGGGGAA
    AACGCGCGGC AGCTGGCCCG AATCGTGTGC GGCACGGTGA TGGCGGGGGA GCTGTCCCTG
    ATGGCAGCAC TGGCAGCAGG CCATCTGGTC AGAAGTCACA TGATCCACAA CAGGTCAAAG
    ATAAATTTGC AAGACCTCCA AGGAACTTGC ACTAAGAAGG CAGCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.