ITSN1 gene cDNA ORF clone, Bubalus bubalis(water buffalo)

mRNA	Protein	Name
XM_025281349.1	XP_025137134.1	intersectin-1 isoform X6
XM_006048225.1	XP_006048287.1	intersectin-1 isoform X1
XM_025281353.1	XP_025137138.1	intersectin-1 isoform X7
XM_025281327.1	XP_025137112.1	intersectin-1 isoform X3
XM_006048225.2	XP_006048287.1	intersectin-1 isoform X4
XM_025281309.1	XP_025137094.1	intersectin-1 isoform X1
XM_025281333.1	XP_025137118.1	intersectin-1 isoform X3
XM_006048226.1	XP_006048288.1	intersectin-1 isoform X2
XM_025281314.1	XP_025137099.1	intersectin-1 isoform X2
XM_025281317.1	XP_025137102.1	intersectin-1 isoform X3
XM_025281323.1	XP_025137108.1	intersectin-1 isoform X3
XM_025281344.1	XP_025137129.1	intersectin-1 isoform X5

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBu06694	XM_006048225.2 Latest version!	Bubalus bubalis intersectin 1 (ITSN1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBu06695	XM_025281317.1 Latest version!	Bubalus bubalis intersectin 1 (ITSN1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBu06695	XM_006048226.1 Latest version!	Bubalus bubalis intersectin 1 (SH3 domain protein) (ITSN1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBu06695	XM_025281323.1 Latest version!	Bubalus bubalis intersectin 1 (ITSN1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBu06695	XM_025281333.1 Latest version!	Bubalus bubalis intersectin 1 (ITSN1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBu06695	XM_025281327.1 Latest version!	Bubalus bubalis intersectin 1 (ITSN1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBu42475	XM_025281309.1 Latest version!	Bubalus bubalis intersectin 1 (ITSN1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBu42476	XM_025281314.1 Latest version!	Bubalus bubalis intersectin 1 (ITSN1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBu42477	XM_025281344.1 Latest version!	Bubalus bubalis intersectin 1 (ITSN1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBu42478	XM_025281349.1 Latest version!	Bubalus bubalis intersectin 1 (ITSN1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBu42479	XM_025281353.1 Latest version!	Bubalus bubalis intersectin 1 (ITSN1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
View Old Accession Versions >>
OBu06694	XM_006048225.1	Bubalus bubalis intersectin 1 (SH3 domain protein) (ITSN1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
Hide Old Accession Versions >>

ITSN1 ( XM_006048225.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006048287.1 Bubalus bubalis intersectin 1 (SH3 domain protein) (ITSN1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBu06694
Clone ID Related Accession (Same CDS sequence)	XM_006048225.1 , XM_006048225.2
Accession Version	XM_006048225.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5151bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	intersectin-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005784098.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCAGT TTCCCACACC TTTTGGTGGC AGCCTGGATA TTTGGGCCAT AACTGTAGAG 
GAAAGAGCAA AGCATGATCA ACAGTTCCAT AGTTTAAAGC CAATATCTGG ATTTATTACT 
GGTGATCAAG CTAGAAACTT TTTTTTTCAA TCTGGGTTAC CTCAGCCTGT TTTAGCACAG 
ATATGGGCGC TCGCTGATAT GAATAATGAT GGAAGGATGG ATCAAGTGGA GTTTTCCATT 
GCTATGAAAC TCATCAAGCT GAAGCTCCAA GGATATCAGC TCCCCTCTGC ACTTCCCCCT 
GTCATGAAAC AGCAACCAGT TGCTATTTCT AGTGCACCAG CGTTTGGTAT GGGAGGTATT 
GCCAGCATGC CGCCGCTGAC CGCTGTTGCT CCTGTGCCAA TGGGCTCCAT TCCCGTTGTT 
GGCATGTCTC CCCCCCTAGT ATCATCTGTT CCTGCAGCAG GGGTGCCCCC CCTGGCCAAC 
GGGGCGCCCC CGGTCATACA GCCTCTGCCG GCATTTGCTC ATCCTGCTGC CACATTGCCA 
AAGAGTTCTT CCTTTAGTAG ATCTGGCCCA GGGTCGCAGT TAAACACAAA ATTACAGAAG 
GCACAATCAT TTGATGTGGC CAGCGTCCCT CCTACAGCTG AGTGGGCTGT TCCTCAGTCA 
TCCAGGCTGA AATACAGACA GCTCTTCAAT AGTCATGACA AAACCATGAG TGGACACCTA 
ACAGGTCCCC AAGCAAGAAC TATTCTTATG CAATCAAGTT TACCACAGGC TCAGCTGGCT 
TCAATATGGA ATCTTTCTGA CATTGATCAA GATGGAAAAC TCACAGCAGA AGAATTTATC 
CTGGCTATGC ACCTCATTGA TGTAGCTATG TCTGGTCAAC CATTGCCACC GGTCCTGCCC 
CCAGAATACA TTCCACCTTC CTTTAGAAGA GTTCGATCTG GTAGTGGTAT ATCTGTCATA 
ACCTCAACTT CGGTGGATCA GAGGTTGCCA GAGGAACCAG TTTTGGAAGA TGAACAGCAA 
CAACTAGAAA AGAAACTACC TGTAACCTTT GAAGATAAGA AGCGGGAGAA CTTTGAACGG 
GGCAACCTGG AGCTGGAGAA GCGGAGACAA GCGCTCTTGG AGCAGCAGCG TAAAGAGCAG 
GAGCGCCTGG CCCAGCTGGA GCGGGCAGAA CAGGAGAGGA AGGAGCGGGA GCGCCAGGAG 
CAGGAGCGCA AAAGACAACT GGAGCTGGAA AAGCAGCTAG AGAAGCAGCG GGAGCTGGAG 
CGGCAGAGGG AGGAGGAGCG AAGGAAGGAA ATCGAGAGGC GGGAGGCTGC GAAACGGGAG 
CTTGAAAGGC AAAGACAGCT TGAATGGGAA CGGAATCGAA GACAAGAACT ATTAAATCAA 
AGAAACAAAG AACAAGAAGA CATAGTTGTA CTGAAAGCAA AGAAAAAGAC TTTGGAATTT 
GAATTAGAAG CTCTAAATGA TAAAAAACAT CAATTAGAAG GAAAACTTCA GGATATCAGA 
TGTCGACTGA CAACCCAAAG GCAAGAAATT GAGAGCACAA ACAAATCTAG AGAGTTGAGA 
ATTGCAGAAA TCACCCACCT ACAACAGCAA TTACAGGAGT CCCAGCAAAT GCTTGGAAGA 
CTTATTCCAG AAAAACAGAT ACTCAATGAC CAATTGAAAC AAGTTCAGCA AAACAGTTTG 
CACAGGGATT CACTTCTCAC ACTCAAAAGA GCCTTAGAAG CGAAAGAACT GGCTCGGCAG 
CAGCTACGAG ACCAACTGGA TGAAGTGGAG AAAGAAACTC GGTCAAAATT ACAGGAGATT 
GATATTTTCA ACAATCAGCT GAAGGAACTT AGAGAAATAC ATAATAAGCA GCAACTCCAG 
AAACAGAAGT CCATAGAGGC TGAACGACTG AAACAGAAGG AACAAGAACG AAAGATCCTG 
GAATTAGAAA AACAAAAAGA AGAAGCCCAA AGACGAGCTC AGGAAAGGGA CCAGCAGTGG 
CTGGAGCGCG TGCATCAGGA GGATGAGCAG CAGCGGCCAC GAAAACCCCA CGAGGAGGAG 
AAGCTCAAAA GGGAAGAGAG CGTCAAAAAG AAGGACAGTG AGGAGAAGGG CAAACCGGAA 
ATGCAAGACA AGCTGAGTCG GCTTTTCCAC CAGCATCAAG AACCAGCTAA GCCCGCTGTC 
CAGGCTCCCT GGTCCACTGC AGAAAAAGGC CCACTTACGA TTTCTGCACA GGAGAATGTA 
AAAGTGGTGT ATTACCGAGC ACTTTATCCC TTTGAGTCCA GGAGTCACGA TGAAATCACG 
ATCCAGCCGG GAGATATAGT CATGGTGGAC GAAAGCCAAA CTGGAGAACC GGGATGGCTT 
GGAGGAGAAT TAAAAGGAAA GACGGGATGG TTCCCTGCAA ACTACGCAGA GAAAATCCCA 
GAAAACGAGG TTCCCGCCCC AGTCAAACCA GTGACTGAGG CGGCCTCCAC CCCTACACCC 
AAAGTGGCTG TGCGTGAGAC CCCCGCCCCT TCATCTGCAA CCCCTGCAGA GCCCTCCGAG 
ACCCCCAACA ACTGGGCCGA CTTCAGCTCC ACGTGGCCCA CCAGCACGAA TGAGAAACCA 
GAAACCGACA GCTGGGACGC ATGGGCAACC CAGCCCTCCC TCACCGTGCC AAGCGCCGGG 
CAGCTGAGGC AGAGGTCGGC CTTCACTCCA GCCACGGCCA CGGGCTCATC TCCGTCTCCT 
GTTCTAGGCC AGGGTGAAAA GGTGGAGGGG CTACAGGCGC AAGCCCTGTA TCCGTGGAGA 
GCCAAGAAAG ACAACCATTT AAATTTTAAC AAAAACGATG TCATCACCGT ACTGGAGCAG 
CAAGACATGT GGTGGTTTGG AGAAGTTCAA GGCCAGAAGG GTTGGTTCCC CAAGTCCTAC 
GTGAAACTCA TTTCAGGGCC CATAAGGAAA TCGACAAGCA TGGATTCTGG TTCCTCAGAA 
AGTCCTGCAA GTCTGAAGAG GGTAGCTTCC CCAGCAGCCA AGCCAGCTGT GTCGGGAGAA 
GAATTTATTG CCATGTACAC TTACGAGAGT TCTGAGCAAG GCGATTTAAC CTTTCAGCAA 
GGGGATGTGA TTTTGGTTAC CAAGAAAGAT GGTGACTGGT GGACAGGAAC AGTGGGCGAC 
AAGTCCGGCG TCTTCCCTTC TAACTATGTG AGGCTTAAAG ATTCAGAGGG CTCTGGAACT 
GCTGGGAAAA CAGGGAGTTT AGGAAAAAAA CCTGAAATCG CACAGGTCAT TGCCTCCTAC 
ACAGCTACAG GTCCTGAACA GCTTACCCTG GCCCCCGGTC AGCTGATCCT GATCCGGAAA 
AAGAACCCAG GTGGATGGTG GGAAGGAGAG CTACAGGCAC GTGGGAAAAA GCGCCAGATA 
GGCTGGTTTC CTGCTAATTA CGTCAAACTT TTAAGCCCTG GGACGAGCAA AATCACTCCA 
ACAGACCCAC CAAAGCCAAC AGCTTTCCCA ACAGTGTGCC AGGTGATCGG GATGTACGAC 
TACACCGCGC AGAACGACGA CGAGCTGGCC TTCAGCAAGG GCCAGGTCAT CAATGTCCTC 
AACAAGGAGG ACCCCGACTG GTGGAAGGGT GAAGTCCACG GGCAGGTGGG GCTCTTCCCG 
TCCAACTACG TGAAGCTGAC CACGGACACG GACCCCAGCC AGCAATGGTG TTCAGACTTA 
CACCTCTTGG ATATGCTGAC CCCAACTGAA AGAAAGCGCC AGGGGTACAT CCACGAGCTC 
ATCGTCACAG AGGAGAACTA TGTGAATGAC CTGCAGCTGG TCACAGAGAT CTTTCAGAAA 
CCCCTGATGG AGTCTGAGCT GCTGACAGAA AAAGAGGGTG CTATGATTTT TGTTAACTGG 
AAGGAGCTGA TTATGTGTAA TATCAAACTA CTGAAAGCGC TGAGGGTCCG CAAGAAGATG 
TCTGGGGAGA AGATGCCCGT GAAGATGATC GGAGACATCC TGACGGCCCA GCTGCCACAC 
ATGCAGCCCT ACATCCGCTT CTGCAGCTGC CAGCTCAACG GGGCCGCCTT GATCCAGCAG 
AAGACTGACG AGGCCCCAGA CTTCAAGGAG TTTGTTAAAA GGCTGGCCAT GGACCCACGC 
TGTAAGGGAA TGCCCCTGTC TAGTTTTATA CTGAAGCCTA TGCAACGAGT AACGAGATAC 
CCACTGATCA TTAAAAATAT CCTGGAAAAC ACTCCCGAAA ACCACCCAGA CCACAGCCAC 
CTGAAGCACG CCCTGGAGAA GGCAGAGGAG CTGTGCTCCC AGGTGAACGA AGGGGTGCGG 
GAGAAGGAGA ACTCGGACCG ACTGGAGTGG ATCCAGGCCC ACGTACTGTG TGAAGGCCTG 
TCAGAGCAAC TTGTGTTCAA TTCCGTGACC AACTGCCTGG GGCCGCGCAA GTTCCTGCAC 
AGTGGGAAGC TCTACAAGGC CAAGAGCAAC AAGGAGCTGT ATGGCTTCCT CTTCAATGAC 
TTCCTGCTGC TGACCCAAAT CATCAAGCCC CTGGGCTCCT CAGGCACCGA CAAAGTCTTC 
AGCCCCAAAT CAAACCTGCA GTATAAAATG TACAAAACAC CTATCTTCCT AAATGAGGTT 
CTAGTGAAGT TGCCCACTGA CCCTTCTGGG GATGAACCCA TCTTCCACAT TTCCCACATC 
GACCGAGTCT ACACCCTCCG AGCAGAGAGC ATAAACGAAA GGACTGCTTG GGTGCAGAAA 
ATCAAAGCAG CTTCTGAACT CTACATAGAG ACAGAGAAGA AGAAGCGGGA GAAGGCGTAT 
CTGGTCCGCT CCCAAAGAGC CACAGGCATC GGAAGGTTGA TGGTGAACGT GGTTGAAGGC 
ATTGAGCTGA AACCCTGTCG CTCCCATGGG AAGAGTAACC CATACTGCGA GGTGACCATG 
GGTTCCCAGT GTCACATCAC CAAGACGATC CAGGACACTC TGAATCCCAA GTGGAATTCC 
AACTGCCAGT TCTTCATCAG AGACTTGGAG CAAGAGGTGC TGTGCATCAC CGTGTTTGAG 
AGGGACCAGT TCTCGCCAGA TGATTTTTTG GGTCGGACGG AGATCCGCGT GGCCGACATC 
AAGAAGGACC AGGGCTCCAA GGGTCCAGTT ACGAAGTGTC TCCTGCTGCA CGAAGTTCCC 
ACGGGAGAGA TCGTGGTCCG CTTGGACCTG CAGTTGTTCG ATGAGCCGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006048287.1
CDS	288..5438
Translation	MAQFPTPFGGSLDIWAITVEERAKHDQQFHSLKPISGFITGDQARNFFFQSGLPQPVLAQIWALADMNNDGRMDQVEFSIAMKLIKLKLQGYQLPSALPPVMKQQPVAISSAPAFGMGGIASMPPLTAVAPVPMGSIPVVGMSPPLVSSVPAAGVPPLANGAPPVIQPLPAFAHPAATLPKSSSFSRSGPGSQLNTKLQKAQSFDVASVPPTAEWAVPQSSRLKYRQLFNSHDKTMSGHLTGPQARTILMQSSLPQAQLASIWNLSDIDQDGKLTAEEFILAMHLIDVAMSGQPLPPVLPPEYIPPSFRRVRSGSGISVITSTSVDQRLPEEPVLEDEQQQLEKKLPVTFEDKKRENFERGNLELEKRRQALLEQQRKEQERLAQLERAEQERKERERQEQERKRQLELEKQLEKQRELERQREEERRKEIERREAAKRELERQRQLEWERNRRQELLNQRNKEQEDIVVLKAKKKTLEFELEALNDKKHQLEGKLQDIRCRLTTQRQEIESTNKSRELRIAEITHLQQQLQESQQMLGRLIPEKQILNDQLKQVQQNSLHRDSLLTLKRALEAKELARQQLRDQLDEVEKETRSKLQEIDIFNNQLKELREIHNKQQLQKQKSIEAERLKQKEQERKILELEKQKEEAQRRAQERDQQWLERVHQEDEQQRPRKPHEEEKLKREESVKKKDSEEKGKPEMQDKLSRLFHQHQEPAKPAVQAPWSTAEKGPLTISAQENVKVVYYRALYPFESRSHDEITIQPGDIVMVDESQTGEPGWLGGELKGKTGWFPANYAEKIPENEVPAPVKPVTEAASTPTPKVAVRETPAPSSATPAEPSETPNNWADFSSTWPTSTNEKPETDSWDAWATQPSLTVPSAGQLRQRSAFTPATATGSSPSPVLGQGEKVEGLQAQALYPWRAKKDNHLNFNKNDVITVLEQQDMWWFGEVQGQKGWFPKSYVKLISGPIRKSTSMDSGSSESPASLKRVASPAAKPAVSGEEFIAMYTYESSEQGDLTFQQGDVILVTKKDGDWWTGTVGDKSGVFPSNYVRLKDSEGSGTAGKTGSLGKKPEIAQVIASYTATGPEQLTLAPGQLILIRKKNPGGWWEGELQARGKKRQIGWFPANYVKLLSPGTSKITPTDPPKPTAFPTVCQVIGMYDYTAQNDDELAFSKGQVINVLNKEDPDWWKGEVHGQVGLFPSNYVKLTTDTDPSQQWCSDLHLLDMLTPTERKRQGYIHELIVTEENYVNDLQLVTEIFQKPLMESELLTEKEGAMIFVNWKELIMCNIKLLKALRVRKKMSGEKMPVKMIGDILTAQLPHMQPYIRFCSCQLNGAALIQQKTDEAPDFKEFVKRLAMDPRCKGMPLSSFILKPMQRVTRYPLIIKNILENTPENHPDHSHLKHALEKAEELCSQVNEGVREKENSDRLEWIQAHVLCEGLSEQLVFNSVTNCLGPRKFLHSGKLYKAKSNKELYGFLFNDFLLLTQIIKPLGSSGTDKVFSPKSNLQYKMYKTPIFLNEVLVKLPTDPSGDEPIFHISHIDRVYTLRAESINERTAWVQKIKAASELYIETEKKKREKAYLVRSQRATGIGRLMVNVVEGIELKPCRSHGKSNPYCEVTMGSQCHITKTIQDTLNPKWNSNCQFFIRDLEQEVLCITVFERDQFSPDDFLGRTEIRVADIKKDQGSKGPVTKCLLLHEVPTGEIVVRLDLQLFDEP

Target ORF information:

RefSeq Version	XM_006048225.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis intersectin 1 (SH3 domain protein) (ITSN1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006048225.1

ORF Insert Sequence:

ATGGCTCAGT TTCCCACACC TTTTGGTGGC AGCCTGGATA TTTGGGCCAT AACTGTAGAG 
GAAAGAGCAA AGCATGATCA ACAGTTCCAT AGTTTAAAGC CAATATCTGG ATTTATTACT 
GGTGATCAAG CTAGAAACTT TTTTTTTCAA TCTGGGTTAC CTCAGCCTGT TTTAGCACAG 
ATATGGGCGC TCGCTGATAT GAATAATGAT GGAAGGATGG ATCAAGTGGA GTTTTCCATT 
GCTATGAAAC TCATCAAGCT GAAGCTCCAA GGATATCAGC TCCCCTCTGC ACTTCCCCCT 
GTCATGAAAC AGCAACCAGT TGCTATTTCT AGTGCACCAG CGTTTGGTAT GGGAGGTATT 
GCCAGCATGC CGCCGCTGAC CGCTGTTGCT CCTGTGCCAA TGGGCTCCAT TCCCGTTGTT 
GGCATGTCTC CCCCCCTAGT ATCATCTGTT CCTGCAGCAG GGGTGCCCCC CCTGGCCAAC 
GGGGCGCCCC CGGTCATACA GCCTCTGCCG GCATTTGCTC ATCCTGCTGC CACATTGCCA 
AAGAGTTCTT CCTTTAGTAG ATCTGGCCCA GGGTCGCAGT TAAACACAAA ATTACAGAAG 
GCACAATCAT TTGATGTGGC CAGCGTCCCT CCTACAGCTG AGTGGGCTGT TCCTCAGTCA 
TCCAGGCTGA AATACAGACA GCTCTTCAAT AGTCATGACA AAACCATGAG TGGACACCTA 
ACAGGTCCCC AAGCAAGAAC TATTCTTATG CAATCAAGTT TACCACAGGC TCAGCTGGCT 
TCAATATGGA ATCTTTCTGA CATTGATCAA GATGGAAAAC TCACAGCAGA AGAATTTATC 
CTGGCTATGC ACCTCATTGA TGTAGCTATG TCTGGTCAAC CATTGCCACC GGTCCTGCCC 
CCAGAATACA TTCCACCTTC CTTTAGAAGA GTTCGATCTG GTAGTGGTAT ATCTGTCATA 
ACCTCAACTT CGGTGGATCA GAGGTTGCCA GAGGAACCAG TTTTGGAAGA TGAACAGCAA 
CAACTAGAAA AGAAACTACC TGTAACCTTT GAAGATAAGA AGCGGGAGAA CTTTGAACGG 
GGCAACCTGG AGCTGGAGAA GCGGAGACAA GCGCTCTTGG AGCAGCAGCG TAAAGAGCAG 
GAGCGCCTGG CCCAGCTGGA GCGGGCAGAA CAGGAGAGGA AGGAGCGGGA GCGCCAGGAG 
CAGGAGCGCA AAAGACAACT GGAGCTGGAA AAGCAGCTAG AGAAGCAGCG GGAGCTGGAG 
CGGCAGAGGG AGGAGGAGCG AAGGAAGGAA ATCGAGAGGC GGGAGGCTGC GAAACGGGAG 
CTTGAAAGGC AAAGACAGCT TGAATGGGAA CGGAATCGAA GACAAGAACT ATTAAATCAA 
AGAAACAAAG AACAAGAAGA CATAGTTGTA CTGAAAGCAA AGAAAAAGAC TTTGGAATTT 
GAATTAGAAG CTCTAAATGA TAAAAAACAT CAATTAGAAG GAAAACTTCA GGATATCAGA 
TGTCGACTGA CAACCCAAAG GCAAGAAATT GAGAGCACAA ACAAATCTAG AGAGTTGAGA 
ATTGCAGAAA TCACCCACCT ACAACAGCAA TTACAGGAGT CCCAGCAAAT GCTTGGAAGA 
CTTATTCCAG AAAAACAGAT ACTCAATGAC CAATTGAAAC AAGTTCAGCA AAACAGTTTG 
CACAGGGATT CACTTCTCAC ACTCAAAAGA GCCTTAGAAG CGAAAGAACT GGCTCGGCAG 
CAGCTACGAG ACCAACTGGA TGAAGTGGAG AAAGAAACTC GGTCAAAATT ACAGGAGATT 
GATATTTTCA ACAATCAGCT GAAGGAACTT AGAGAAATAC ATAATAAGCA GCAACTCCAG 
AAACAGAAGT CCATAGAGGC TGAACGACTG AAACAGAAGG AACAAGAACG AAAGATCCTG 
GAATTAGAAA AACAAAAAGA AGAAGCCCAA AGACGAGCTC AGGAAAGGGA CCAGCAGTGG 
CTGGAGCGCG TGCATCAGGA GGATGAGCAG CAGCGGCCAC GAAAACCCCA CGAGGAGGAG 
AAGCTCAAAA GGGAAGAGAG CGTCAAAAAG AAGGACAGTG AGGAGAAGGG CAAACCGGAA 
ATGCAAGACA AGCTGAGTCG GCTTTTCCAC CAGCATCAAG AACCAGCTAA GCCCGCTGTC 
CAGGCTCCCT GGTCCACTGC AGAAAAAGGC CCACTTACGA TTTCTGCACA GGAGAATGTA 
AAAGTGGTGT ATTACCGAGC ACTTTATCCC TTTGAGTCCA GGAGTCACGA TGAAATCACG 
ATCCAGCCGG GAGATATAGT CATGGTGGAC GAAAGCCAAA CTGGAGAACC GGGATGGCTT 
GGAGGAGAAT TAAAAGGAAA GACGGGATGG TTCCCTGCAA ACTACGCAGA GAAAATCCCA 
GAAAACGAGG TTCCCGCCCC AGTCAAACCA GTGACTGAGG CGGCCTCCAC CCCTACACCC 
AAAGTGGCTG TGCGTGAGAC CCCCGCCCCT TCATCTGCAA CCCCTGCAGA GCCCTCCGAG 
ACCCCCAACA ACTGGGCCGA CTTCAGCTCC ACGTGGCCCA CCAGCACGAA TGAGAAACCA 
GAAACCGACA GCTGGGACGC ATGGGCAACC CAGCCCTCCC TCACCGTGCC AAGCGCCGGG 
CAGCTGAGGC AGAGGTCGGC CTTCACTCCA GCCACGGCCA CGGGCTCATC TCCGTCTCCT 
GTTCTAGGCC AGGGTGAAAA GGTGGAGGGG CTACAGGCGC AAGCCCTGTA TCCGTGGAGA 
GCCAAGAAAG ACAACCATTT AAATTTTAAC AAAAACGATG TCATCACCGT ACTGGAGCAG 
CAAGACATGT GGTGGTTTGG AGAAGTTCAA GGCCAGAAGG GTTGGTTCCC CAAGTCCTAC 
GTGAAACTCA TTTCAGGGCC CATAAGGAAA TCGACAAGCA TGGATTCTGG TTCCTCAGAA 
AGTCCTGCAA GTCTGAAGAG GGTAGCTTCC CCAGCAGCCA AGCCAGCTGT GTCGGGAGAA 
GAATTTATTG CCATGTACAC TTACGAGAGT TCTGAGCAAG GCGATTTAAC CTTTCAGCAA 
GGGGATGTGA TTTTGGTTAC CAAGAAAGAT GGTGACTGGT GGACAGGAAC AGTGGGCGAC 
AAGTCCGGCG TCTTCCCTTC TAACTATGTG AGGCTTAAAG ATTCAGAGGG CTCTGGAACT 
GCTGGGAAAA CAGGGAGTTT AGGAAAAAAA CCTGAAATCG CACAGGTCAT TGCCTCCTAC 
ACAGCTACAG GTCCTGAACA GCTTACCCTG GCCCCCGGTC AGCTGATCCT GATCCGGAAA 
AAGAACCCAG GTGGATGGTG GGAAGGAGAG CTACAGGCAC GTGGGAAAAA GCGCCAGATA 
GGCTGGTTTC CTGCTAATTA CGTCAAACTT TTAAGCCCTG GGACGAGCAA AATCACTCCA 
ACAGACCCAC CAAAGCCAAC AGCTTTCCCA ACAGTGTGCC AGGTGATCGG GATGTACGAC 
TACACCGCGC AGAACGACGA CGAGCTGGCC TTCAGCAAGG GCCAGGTCAT CAATGTCCTC 
AACAAGGAGG ACCCCGACTG GTGGAAGGGT GAAGTCCACG GGCAGGTGGG GCTCTTCCCG 
TCCAACTACG TGAAGCTGAC CACGGACACG GACCCCAGCC AGCAATGGTG TTCAGACTTA 
CACCTCTTGG ATATGCTGAC CCCAACTGAA AGAAAGCGCC AGGGGTACAT CCACGAGCTC 
ATCGTCACAG AGGAGAACTA TGTGAATGAC CTGCAGCTGG TCACAGAGAT CTTTCAGAAA 
CCCCTGATGG AGTCTGAGCT GCTGACAGAA AAAGAGGGTG CTATGATTTT TGTTAACTGG 
AAGGAGCTGA TTATGTGTAA TATCAAACTA CTGAAAGCGC TGAGGGTCCG CAAGAAGATG 
TCTGGGGAGA AGATGCCCGT GAAGATGATC GGAGACATCC TGACGGCCCA GCTGCCACAC 
ATGCAGCCCT ACATCCGCTT CTGCAGCTGC CAGCTCAACG GGGCCGCCTT GATCCAGCAG 
AAGACTGACG AGGCCCCAGA CTTCAAGGAG TTTGTTAAAA GGCTGGCCAT GGACCCACGC 
TGTAAGGGAA TGCCCCTGTC TAGTTTTATA CTGAAGCCTA TGCAACGAGT AACGAGATAC 
CCACTGATCA TTAAAAATAT CCTGGAAAAC ACTCCCGAAA ACCACCCAGA CCACAGCCAC 
CTGAAGCACG CCCTGGAGAA GGCAGAGGAG CTGTGCTCCC AGGTGAACGA AGGGGTGCGG 
GAGAAGGAGA ACTCGGACCG ACTGGAGTGG ATCCAGGCCC ACGTACTGTG TGAAGGCCTG 
TCAGAGCAAC TTGTGTTCAA TTCCGTGACC AACTGCCTGG GGCCGCGCAA GTTCCTGCAC 
AGTGGGAAGC TCTACAAGGC CAAGAGCAAC AAGGAGCTGT ATGGCTTCCT CTTCAATGAC 
TTCCTGCTGC TGACCCAAAT CATCAAGCCC CTGGGCTCCT CAGGCACCGA CAAAGTCTTC 
AGCCCCAAAT CAAACCTGCA GTATAAAATG TACAAAACAC CTATCTTCCT AAATGAGGTT 
CTAGTGAAGT TGCCCACTGA CCCTTCTGGG GATGAACCCA TCTTCCACAT TTCCCACATC 
GACCGAGTCT ACACCCTCCG AGCAGAGAGC ATAAACGAAA GGACTGCTTG GGTGCAGAAA 
ATCAAAGCAG CTTCTGAACT CTACATAGAG ACAGAGAAGA AGAAGCGGGA GAAGGCGTAT 
CTGGTCCGCT CCCAAAGAGC CACAGGCATC GGAAGGTTGA TGGTGAACGT GGTTGAAGGC 
ATTGAGCTGA AACCCTGTCG CTCCCATGGG AAGAGTAACC CATACTGCGA GGTGACCATG 
GGTTCCCAGT GTCACATCAC CAAGACGATC CAGGACACTC TGAATCCCAA GTGGAATTCC 
AACTGCCAGT TCTTCATCAG AGACTTGGAG CAAGAGGTGC TGTGCATCAC CGTGTTTGAG 
AGGGACCAGT TCTCGCCAGA TGATTTTTTG GGTCGGACGG AGATCCGCGT GGCCGACATC 
AAGAAGGACC AGGGCTCCAA GGGTCCAGTT ACGAAGTGTC TCCTGCTGCA CGAAGTTCCC 
ACGGGAGAGA TCGTGGTCCG CTTGGACCTG CAGTTGTTCG ATGAGCCGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITSN1 ( XM_006048225.2 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006048287.1 Bubalus bubalis intersectin 1 (ITSN1), transcript variant X7, mRNA.

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CloneID	OBu06694
Clone ID Related Accession (Same CDS sequence)	XM_006048225.1 , XM_006048225.2
Accession Version	XM_006048225.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5151bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	intersectin-1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037545.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006048225.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCAGT TTCCCACACC TTTTGGTGGC AGCCTGGATA TTTGGGCCAT AACTGTAGAG 
GAAAGAGCAA AGCATGATCA ACAGTTCCAT AGTTTAAAGC CAATATCTGG ATTTATTACT 
GGTGATCAAG CTAGAAACTT TTTTTTTCAA TCTGGGTTAC CTCAGCCTGT TTTAGCACAG 
ATATGGGCGC TCGCTGATAT GAATAATGAT GGAAGGATGG ATCAAGTGGA GTTTTCCATT 
GCTATGAAAC TCATCAAGCT GAAGCTCCAA GGATATCAGC TCCCCTCTGC ACTTCCCCCT 
GTCATGAAAC AGCAACCAGT TGCTATTTCT AGTGCACCAG CGTTTGGTAT GGGAGGTATT 
GCCAGCATGC CGCCGCTGAC CGCTGTTGCT CCTGTGCCAA TGGGCTCCAT TCCCGTTGTT 
GGCATGTCTC CCCCCCTAGT ATCATCTGTT CCTGCAGCAG GGGTGCCCCC CCTGGCCAAC 
GGGGCGCCCC CGGTCATACA GCCTCTGCCG GCATTTGCTC ATCCTGCTGC CACATTGCCA 
AAGAGTTCTT CCTTTAGTAG ATCTGGCCCA GGGTCGCAGT TAAACACAAA ATTACAGAAG 
GCACAATCAT TTGATGTGGC CAGCGTCCCT CCTACAGCTG AGTGGGCTGT TCCTCAGTCA 
TCCAGGCTGA AATACAGACA GCTCTTCAAT AGTCATGACA AAACCATGAG TGGACACCTA 
ACAGGTCCCC AAGCAAGAAC TATTCTTATG CAATCAAGTT TACCACAGGC TCAGCTGGCT 
TCAATATGGA ATCTTTCTGA CATTGATCAA GATGGAAAAC TCACAGCAGA AGAATTTATC 
CTGGCTATGC ACCTCATTGA TGTAGCTATG TCTGGTCAAC CATTGCCACC GGTCCTGCCC 
CCAGAATACA TTCCACCTTC CTTTAGAAGA GTTCGATCTG GTAGTGGTAT ATCTGTCATA 
ACCTCAACTT CGGTGGATCA GAGGTTGCCA GAGGAACCAG TTTTGGAAGA TGAACAGCAA 
CAACTAGAAA AGAAACTACC TGTAACCTTT GAAGATAAGA AGCGGGAGAA CTTTGAACGG 
GGCAACCTGG AGCTGGAGAA GCGGAGACAA GCGCTCTTGG AGCAGCAGCG TAAAGAGCAG 
GAGCGCCTGG CCCAGCTGGA GCGGGCAGAA CAGGAGAGGA AGGAGCGGGA GCGCCAGGAG 
CAGGAGCGCA AAAGACAACT GGAGCTGGAA AAGCAGCTAG AGAAGCAGCG GGAGCTGGAG 
CGGCAGAGGG AGGAGGAGCG AAGGAAGGAA ATCGAGAGGC GGGAGGCTGC GAAACGGGAG 
CTTGAAAGGC AAAGACAGCT TGAATGGGAA CGGAATCGAA GACAAGAACT ATTAAATCAA 
AGAAACAAAG AACAAGAAGA CATAGTTGTA CTGAAAGCAA AGAAAAAGAC TTTGGAATTT 
GAATTAGAAG CTCTAAATGA TAAAAAACAT CAATTAGAAG GAAAACTTCA GGATATCAGA 
TGTCGACTGA CAACCCAAAG GCAAGAAATT GAGAGCACAA ACAAATCTAG AGAGTTGAGA 
ATTGCAGAAA TCACCCACCT ACAACAGCAA TTACAGGAGT CCCAGCAAAT GCTTGGAAGA 
CTTATTCCAG AAAAACAGAT ACTCAATGAC CAATTGAAAC AAGTTCAGCA AAACAGTTTG 
CACAGGGATT CACTTCTCAC ACTCAAAAGA GCCTTAGAAG CGAAAGAACT GGCTCGGCAG 
CAGCTACGAG ACCAACTGGA TGAAGTGGAG AAAGAAACTC GGTCAAAATT ACAGGAGATT 
GATATTTTCA ACAATCAGCT GAAGGAACTT AGAGAAATAC ATAATAAGCA GCAACTCCAG 
AAACAGAAGT CCATAGAGGC TGAACGACTG AAACAGAAGG AACAAGAACG AAAGATCCTG 
GAATTAGAAA AACAAAAAGA AGAAGCCCAA AGACGAGCTC AGGAAAGGGA CCAGCAGTGG 
CTGGAGCGCG TGCATCAGGA GGATGAGCAG CAGCGGCCAC GAAAACCCCA CGAGGAGGAG 
AAGCTCAAAA GGGAAGAGAG CGTCAAAAAG AAGGACAGTG AGGAGAAGGG CAAACCGGAA 
ATGCAAGACA AGCTGAGTCG GCTTTTCCAC CAGCATCAAG AACCAGCTAA GCCCGCTGTC 
CAGGCTCCCT GGTCCACTGC AGAAAAAGGC CCACTTACGA TTTCTGCACA GGAGAATGTA 
AAAGTGGTGT ATTACCGAGC ACTTTATCCC TTTGAGTCCA GGAGTCACGA TGAAATCACG 
ATCCAGCCGG GAGATATAGT CATGGTGGAC GAAAGCCAAA CTGGAGAACC GGGATGGCTT 
GGAGGAGAAT TAAAAGGAAA GACGGGATGG TTCCCTGCAA ACTACGCAGA GAAAATCCCA 
GAAAACGAGG TTCCCGCCCC AGTCAAACCA GTGACTGAGG CGGCCTCCAC CCCTACACCC 
AAAGTGGCTG TGCGTGAGAC CCCCGCCCCT TCATCTGCAA CCCCTGCAGA GCCCTCCGAG 
ACCCCCAACA ACTGGGCCGA CTTCAGCTCC ACGTGGCCCA CCAGCACGAA TGAGAAACCA 
GAAACCGACA GCTGGGACGC ATGGGCAACC CAGCCCTCCC TCACCGTGCC AAGCGCCGGG 
CAGCTGAGGC AGAGGTCGGC CTTCACTCCA GCCACGGCCA CGGGCTCATC TCCGTCTCCT 
GTTCTAGGCC AGGGTGAAAA GGTGGAGGGG CTACAGGCGC AAGCCCTGTA TCCGTGGAGA 
GCCAAGAAAG ACAACCATTT AAATTTTAAC AAAAACGATG TCATCACCGT ACTGGAGCAG 
CAAGACATGT GGTGGTTTGG AGAAGTTCAA GGCCAGAAGG GTTGGTTCCC CAAGTCCTAC 
GTGAAACTCA TTTCAGGGCC CATAAGGAAA TCGACAAGCA TGGATTCTGG TTCCTCAGAA 
AGTCCTGCAA GTCTGAAGAG GGTAGCTTCC CCAGCAGCCA AGCCAGCTGT GTCGGGAGAA 
GAATTTATTG CCATGTACAC TTACGAGAGT TCTGAGCAAG GCGATTTAAC CTTTCAGCAA 
GGGGATGTGA TTTTGGTTAC CAAGAAAGAT GGTGACTGGT GGACAGGAAC AGTGGGCGAC 
AAGTCCGGCG TCTTCCCTTC TAACTATGTG AGGCTTAAAG ATTCAGAGGG CTCTGGAACT 
GCTGGGAAAA CAGGGAGTTT AGGAAAAAAA CCTGAAATCG CACAGGTCAT TGCCTCCTAC 
ACAGCTACAG GTCCTGAACA GCTTACCCTG GCCCCCGGTC AGCTGATCCT GATCCGGAAA 
AAGAACCCAG GTGGATGGTG GGAAGGAGAG CTACAGGCAC GTGGGAAAAA GCGCCAGATA 
GGCTGGTTTC CTGCTAATTA CGTCAAACTT TTAAGCCCTG GGACGAGCAA AATCACTCCA 
ACAGACCCAC CAAAGCCAAC AGCTTTCCCA ACAGTGTGCC AGGTGATCGG GATGTACGAC 
TACACCGCGC AGAACGACGA CGAGCTGGCC TTCAGCAAGG GCCAGGTCAT CAATGTCCTC 
AACAAGGAGG ACCCCGACTG GTGGAAGGGT GAAGTCCACG GGCAGGTGGG GCTCTTCCCG 
TCCAACTACG TGAAGCTGAC CACGGACACG GACCCCAGCC AGCAATGGTG TTCAGACTTA 
CACCTCTTGG ATATGCTGAC CCCAACTGAA AGAAAGCGCC AGGGGTACAT CCACGAGCTC 
ATCGTCACAG AGGAGAACTA TGTGAATGAC CTGCAGCTGG TCACAGAGAT CTTTCAGAAA 
CCCCTGATGG AGTCTGAGCT GCTGACAGAA AAAGAGGGTG CTATGATTTT TGTTAACTGG 
AAGGAGCTGA TTATGTGTAA TATCAAACTA CTGAAAGCGC TGAGGGTCCG CAAGAAGATG 
TCTGGGGAGA AGATGCCCGT GAAGATGATC GGAGACATCC TGACGGCCCA GCTGCCACAC 
ATGCAGCCCT ACATCCGCTT CTGCAGCTGC CAGCTCAACG GGGCCGCCTT GATCCAGCAG 
AAGACTGACG AGGCCCCAGA CTTCAAGGAG TTTGTTAAAA GGCTGGCCAT GGACCCACGC 
TGTAAGGGAA TGCCCCTGTC TAGTTTTATA CTGAAGCCTA TGCAACGAGT AACGAGATAC 
CCACTGATCA TTAAAAATAT CCTGGAAAAC ACTCCCGAAA ACCACCCAGA CCACAGCCAC 
CTGAAGCACG CCCTGGAGAA GGCAGAGGAG CTGTGCTCCC AGGTGAACGA AGGGGTGCGG 
GAGAAGGAGA ACTCGGACCG ACTGGAGTGG ATCCAGGCCC ACGTACTGTG TGAAGGCCTG 
TCAGAGCAAC TTGTGTTCAA TTCCGTGACC AACTGCCTGG GGCCGCGCAA GTTCCTGCAC 
AGTGGGAAGC TCTACAAGGC CAAGAGCAAC AAGGAGCTGT ATGGCTTCCT CTTCAATGAC 
TTCCTGCTGC TGACCCAAAT CATCAAGCCC CTGGGCTCCT CAGGCACCGA CAAAGTCTTC 
AGCCCCAAAT CAAACCTGCA GTATAAAATG TACAAAACAC CTATCTTCCT AAATGAGGTT 
CTAGTGAAGT TGCCCACTGA CCCTTCTGGG GATGAACCCA TCTTCCACAT TTCCCACATC 
GACCGAGTCT ACACCCTCCG AGCAGAGAGC ATAAACGAAA GGACTGCTTG GGTGCAGAAA 
ATCAAAGCAG CTTCTGAACT CTACATAGAG ACAGAGAAGA AGAAGCGGGA GAAGGCGTAT 
CTGGTCCGCT CCCAAAGAGC CACAGGCATC GGAAGGTTGA TGGTGAACGT GGTTGAAGGC 
ATTGAGCTGA AACCCTGTCG CTCCCATGGG AAGAGTAACC CATACTGCGA GGTGACCATG 
GGTTCCCAGT GTCACATCAC CAAGACGATC CAGGACACTC TGAATCCCAA GTGGAATTCC 
AACTGCCAGT TCTTCATCAG AGACTTGGAG CAAGAGGTGC TGTGCATCAC CGTGTTTGAG 
AGGGACCAGT TCTCGCCAGA TGATTTTTTG GGTCGGACGG AGATCCGCGT GGCCGACATC 
AAGAAGGACC AGGGCTCCAA GGGTCCAGTT ACGAAGTGTC TCCTGCTGCA CGAAGTTCCC 
ACGGGAGAGA TCGTGGTCCG CTTGGACCTG CAGTTGTTCG ATGAGCCGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006048287.1
CDS	301..5451
Translation	MAQFPTPFGGSLDIWAITVEERAKHDQQFHSLKPISGFITGDQARNFFFQSGLPQPVLAQIWALADMNNDGRMDQVEFSIAMKLIKLKLQGYQLPSALPPVMKQQPVAISSAPAFGMGGIASMPPLTAVAPVPMGSIPVVGMSPPLVSSVPAAGVPPLANGAPPVIQPLPAFAHPAATLPKSSSFSRSGPGSQLNTKLQKAQSFDVASVPPTAEWAVPQSSRLKYRQLFNSHDKTMSGHLTGPQARTILMQSSLPQAQLASIWNLSDIDQDGKLTAEEFILAMHLIDVAMSGQPLPPVLPPEYIPPSFRRVRSGSGISVITSTSVDQRLPEEPVLEDEQQQLEKKLPVTFEDKKRENFERGNLELEKRRQALLEQQRKEQERLAQLERAEQERKERERQEQERKRQLELEKQLEKQRELERQREEERRKEIERREAAKRELERQRQLEWERNRRQELLNQRNKEQEDIVVLKAKKKTLEFELEALNDKKHQLEGKLQDIRCRLTTQRQEIESTNKSRELRIAEITHLQQQLQESQQMLGRLIPEKQILNDQLKQVQQNSLHRDSLLTLKRALEAKELARQQLRDQLDEVEKETRSKLQEIDIFNNQLKELREIHNKQQLQKQKSIEAERLKQKEQERKILELEKQKEEAQRRAQERDQQWLERVHQEDEQQRPRKPHEEEKLKREESVKKKDSEEKGKPEMQDKLSRLFHQHQEPAKPAVQAPWSTAEKGPLTISAQENVKVVYYRALYPFESRSHDEITIQPGDIVMVDESQTGEPGWLGGELKGKTGWFPANYAEKIPENEVPAPVKPVTEAASTPTPKVAVRETPAPSSATPAEPSETPNNWADFSSTWPTSTNEKPETDSWDAWATQPSLTVPSAGQLRQRSAFTPATATGSSPSPVLGQGEKVEGLQAQALYPWRAKKDNHLNFNKNDVITVLEQQDMWWFGEVQGQKGWFPKSYVKLISGPIRKSTSMDSGSSESPASLKRVASPAAKPAVSGEEFIAMYTYESSEQGDLTFQQGDVILVTKKDGDWWTGTVGDKSGVFPSNYVRLKDSEGSGTAGKTGSLGKKPEIAQVIASYTATGPEQLTLAPGQLILIRKKNPGGWWEGELQARGKKRQIGWFPANYVKLLSPGTSKITPTDPPKPTAFPTVCQVIGMYDYTAQNDDELAFSKGQVINVLNKEDPDWWKGEVHGQVGLFPSNYVKLTTDTDPSQQWCSDLHLLDMLTPTERKRQGYIHELIVTEENYVNDLQLVTEIFQKPLMESELLTEKEGAMIFVNWKELIMCNIKLLKALRVRKKMSGEKMPVKMIGDILTAQLPHMQPYIRFCSCQLNGAALIQQKTDEAPDFKEFVKRLAMDPRCKGMPLSSFILKPMQRVTRYPLIIKNILENTPENHPDHSHLKHALEKAEELCSQVNEGVREKENSDRLEWIQAHVLCEGLSEQLVFNSVTNCLGPRKFLHSGKLYKAKSNKELYGFLFNDFLLLTQIIKPLGSSGTDKVFSPKSNLQYKMYKTPIFLNEVLVKLPTDPSGDEPIFHISHIDRVYTLRAESINERTAWVQKIKAASELYIETEKKKREKAYLVRSQRATGIGRLMVNVVEGIELKPCRSHGKSNPYCEVTMGSQCHITKTIQDTLNPKWNSNCQFFIRDLEQEVLCITVFERDQFSPDDFLGRTEIRVADIKKDQGSKGPVTKCLLLHEVPTGEIVVRLDLQLFDEP

Target ORF information:

RefSeq Version	XM_006048225.2
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis intersectin 1 (ITSN1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006048225.2

ORF Insert Sequence:

ATGGCTCAGT TTCCCACACC TTTTGGTGGC AGCCTGGATA TTTGGGCCAT AACTGTAGAG 
GAAAGAGCAA AGCATGATCA ACAGTTCCAT AGTTTAAAGC CAATATCTGG ATTTATTACT 
GGTGATCAAG CTAGAAACTT TTTTTTTCAA TCTGGGTTAC CTCAGCCTGT TTTAGCACAG 
ATATGGGCGC TCGCTGATAT GAATAATGAT GGAAGGATGG ATCAAGTGGA GTTTTCCATT 
GCTATGAAAC TCATCAAGCT GAAGCTCCAA GGATATCAGC TCCCCTCTGC ACTTCCCCCT 
GTCATGAAAC AGCAACCAGT TGCTATTTCT AGTGCACCAG CGTTTGGTAT GGGAGGTATT 
GCCAGCATGC CGCCGCTGAC CGCTGTTGCT CCTGTGCCAA TGGGCTCCAT TCCCGTTGTT 
GGCATGTCTC CCCCCCTAGT ATCATCTGTT CCTGCAGCAG GGGTGCCCCC CCTGGCCAAC 
GGGGCGCCCC CGGTCATACA GCCTCTGCCG GCATTTGCTC ATCCTGCTGC CACATTGCCA 
AAGAGTTCTT CCTTTAGTAG ATCTGGCCCA GGGTCGCAGT TAAACACAAA ATTACAGAAG 
GCACAATCAT TTGATGTGGC CAGCGTCCCT CCTACAGCTG AGTGGGCTGT TCCTCAGTCA 
TCCAGGCTGA AATACAGACA GCTCTTCAAT AGTCATGACA AAACCATGAG TGGACACCTA 
ACAGGTCCCC AAGCAAGAAC TATTCTTATG CAATCAAGTT TACCACAGGC TCAGCTGGCT 
TCAATATGGA ATCTTTCTGA CATTGATCAA GATGGAAAAC TCACAGCAGA AGAATTTATC 
CTGGCTATGC ACCTCATTGA TGTAGCTATG TCTGGTCAAC CATTGCCACC GGTCCTGCCC 
CCAGAATACA TTCCACCTTC CTTTAGAAGA GTTCGATCTG GTAGTGGTAT ATCTGTCATA 
ACCTCAACTT CGGTGGATCA GAGGTTGCCA GAGGAACCAG TTTTGGAAGA TGAACAGCAA 
CAACTAGAAA AGAAACTACC TGTAACCTTT GAAGATAAGA AGCGGGAGAA CTTTGAACGG 
GGCAACCTGG AGCTGGAGAA GCGGAGACAA GCGCTCTTGG AGCAGCAGCG TAAAGAGCAG 
GAGCGCCTGG CCCAGCTGGA GCGGGCAGAA CAGGAGAGGA AGGAGCGGGA GCGCCAGGAG 
CAGGAGCGCA AAAGACAACT GGAGCTGGAA AAGCAGCTAG AGAAGCAGCG GGAGCTGGAG 
CGGCAGAGGG AGGAGGAGCG AAGGAAGGAA ATCGAGAGGC GGGAGGCTGC GAAACGGGAG 
CTTGAAAGGC AAAGACAGCT TGAATGGGAA CGGAATCGAA GACAAGAACT ATTAAATCAA 
AGAAACAAAG AACAAGAAGA CATAGTTGTA CTGAAAGCAA AGAAAAAGAC TTTGGAATTT 
GAATTAGAAG CTCTAAATGA TAAAAAACAT CAATTAGAAG GAAAACTTCA GGATATCAGA 
TGTCGACTGA CAACCCAAAG GCAAGAAATT GAGAGCACAA ACAAATCTAG AGAGTTGAGA 
ATTGCAGAAA TCACCCACCT ACAACAGCAA TTACAGGAGT CCCAGCAAAT GCTTGGAAGA 
CTTATTCCAG AAAAACAGAT ACTCAATGAC CAATTGAAAC AAGTTCAGCA AAACAGTTTG 
CACAGGGATT CACTTCTCAC ACTCAAAAGA GCCTTAGAAG CGAAAGAACT GGCTCGGCAG 
CAGCTACGAG ACCAACTGGA TGAAGTGGAG AAAGAAACTC GGTCAAAATT ACAGGAGATT 
GATATTTTCA ACAATCAGCT GAAGGAACTT AGAGAAATAC ATAATAAGCA GCAACTCCAG 
AAACAGAAGT CCATAGAGGC TGAACGACTG AAACAGAAGG AACAAGAACG AAAGATCCTG 
GAATTAGAAA AACAAAAAGA AGAAGCCCAA AGACGAGCTC AGGAAAGGGA CCAGCAGTGG 
CTGGAGCGCG TGCATCAGGA GGATGAGCAG CAGCGGCCAC GAAAACCCCA CGAGGAGGAG 
AAGCTCAAAA GGGAAGAGAG CGTCAAAAAG AAGGACAGTG AGGAGAAGGG CAAACCGGAA 
ATGCAAGACA AGCTGAGTCG GCTTTTCCAC CAGCATCAAG AACCAGCTAA GCCCGCTGTC 
CAGGCTCCCT GGTCCACTGC AGAAAAAGGC CCACTTACGA TTTCTGCACA GGAGAATGTA 
AAAGTGGTGT ATTACCGAGC ACTTTATCCC TTTGAGTCCA GGAGTCACGA TGAAATCACG 
ATCCAGCCGG GAGATATAGT CATGGTGGAC GAAAGCCAAA CTGGAGAACC GGGATGGCTT 
GGAGGAGAAT TAAAAGGAAA GACGGGATGG TTCCCTGCAA ACTACGCAGA GAAAATCCCA 
GAAAACGAGG TTCCCGCCCC AGTCAAACCA GTGACTGAGG CGGCCTCCAC CCCTACACCC 
AAAGTGGCTG TGCGTGAGAC CCCCGCCCCT TCATCTGCAA CCCCTGCAGA GCCCTCCGAG 
ACCCCCAACA ACTGGGCCGA CTTCAGCTCC ACGTGGCCCA CCAGCACGAA TGAGAAACCA 
GAAACCGACA GCTGGGACGC ATGGGCAACC CAGCCCTCCC TCACCGTGCC AAGCGCCGGG 
CAGCTGAGGC AGAGGTCGGC CTTCACTCCA GCCACGGCCA CGGGCTCATC TCCGTCTCCT 
GTTCTAGGCC AGGGTGAAAA GGTGGAGGGG CTACAGGCGC AAGCCCTGTA TCCGTGGAGA 
GCCAAGAAAG ACAACCATTT AAATTTTAAC AAAAACGATG TCATCACCGT ACTGGAGCAG 
CAAGACATGT GGTGGTTTGG AGAAGTTCAA GGCCAGAAGG GTTGGTTCCC CAAGTCCTAC 
GTGAAACTCA TTTCAGGGCC CATAAGGAAA TCGACAAGCA TGGATTCTGG TTCCTCAGAA 
AGTCCTGCAA GTCTGAAGAG GGTAGCTTCC CCAGCAGCCA AGCCAGCTGT GTCGGGAGAA 
GAATTTATTG CCATGTACAC TTACGAGAGT TCTGAGCAAG GCGATTTAAC CTTTCAGCAA 
GGGGATGTGA TTTTGGTTAC CAAGAAAGAT GGTGACTGGT GGACAGGAAC AGTGGGCGAC 
AAGTCCGGCG TCTTCCCTTC TAACTATGTG AGGCTTAAAG ATTCAGAGGG CTCTGGAACT 
GCTGGGAAAA CAGGGAGTTT AGGAAAAAAA CCTGAAATCG CACAGGTCAT TGCCTCCTAC 
ACAGCTACAG GTCCTGAACA GCTTACCCTG GCCCCCGGTC AGCTGATCCT GATCCGGAAA 
AAGAACCCAG GTGGATGGTG GGAAGGAGAG CTACAGGCAC GTGGGAAAAA GCGCCAGATA 
GGCTGGTTTC CTGCTAATTA CGTCAAACTT TTAAGCCCTG GGACGAGCAA AATCACTCCA 
ACAGACCCAC CAAAGCCAAC AGCTTTCCCA ACAGTGTGCC AGGTGATCGG GATGTACGAC 
TACACCGCGC AGAACGACGA CGAGCTGGCC TTCAGCAAGG GCCAGGTCAT CAATGTCCTC 
AACAAGGAGG ACCCCGACTG GTGGAAGGGT GAAGTCCACG GGCAGGTGGG GCTCTTCCCG 
TCCAACTACG TGAAGCTGAC CACGGACACG GACCCCAGCC AGCAATGGTG TTCAGACTTA 
CACCTCTTGG ATATGCTGAC CCCAACTGAA AGAAAGCGCC AGGGGTACAT CCACGAGCTC 
ATCGTCACAG AGGAGAACTA TGTGAATGAC CTGCAGCTGG TCACAGAGAT CTTTCAGAAA 
CCCCTGATGG AGTCTGAGCT GCTGACAGAA AAAGAGGGTG CTATGATTTT TGTTAACTGG 
AAGGAGCTGA TTATGTGTAA TATCAAACTA CTGAAAGCGC TGAGGGTCCG CAAGAAGATG 
TCTGGGGAGA AGATGCCCGT GAAGATGATC GGAGACATCC TGACGGCCCA GCTGCCACAC 
ATGCAGCCCT ACATCCGCTT CTGCAGCTGC CAGCTCAACG GGGCCGCCTT GATCCAGCAG 
AAGACTGACG AGGCCCCAGA CTTCAAGGAG TTTGTTAAAA GGCTGGCCAT GGACCCACGC 
TGTAAGGGAA TGCCCCTGTC TAGTTTTATA CTGAAGCCTA TGCAACGAGT AACGAGATAC 
CCACTGATCA TTAAAAATAT CCTGGAAAAC ACTCCCGAAA ACCACCCAGA CCACAGCCAC 
CTGAAGCACG CCCTGGAGAA GGCAGAGGAG CTGTGCTCCC AGGTGAACGA AGGGGTGCGG 
GAGAAGGAGA ACTCGGACCG ACTGGAGTGG ATCCAGGCCC ACGTACTGTG TGAAGGCCTG 
TCAGAGCAAC TTGTGTTCAA TTCCGTGACC AACTGCCTGG GGCCGCGCAA GTTCCTGCAC 
AGTGGGAAGC TCTACAAGGC CAAGAGCAAC AAGGAGCTGT ATGGCTTCCT CTTCAATGAC 
TTCCTGCTGC TGACCCAAAT CATCAAGCCC CTGGGCTCCT CAGGCACCGA CAAAGTCTTC 
AGCCCCAAAT CAAACCTGCA GTATAAAATG TACAAAACAC CTATCTTCCT AAATGAGGTT 
CTAGTGAAGT TGCCCACTGA CCCTTCTGGG GATGAACCCA TCTTCCACAT TTCCCACATC 
GACCGAGTCT ACACCCTCCG AGCAGAGAGC ATAAACGAAA GGACTGCTTG GGTGCAGAAA 
ATCAAAGCAG CTTCTGAACT CTACATAGAG ACAGAGAAGA AGAAGCGGGA GAAGGCGTAT 
CTGGTCCGCT CCCAAAGAGC CACAGGCATC GGAAGGTTGA TGGTGAACGT GGTTGAAGGC 
ATTGAGCTGA AACCCTGTCG CTCCCATGGG AAGAGTAACC CATACTGCGA GGTGACCATG 
GGTTCCCAGT GTCACATCAC CAAGACGATC CAGGACACTC TGAATCCCAA GTGGAATTCC 
AACTGCCAGT TCTTCATCAG AGACTTGGAG CAAGAGGTGC TGTGCATCAC CGTGTTTGAG 
AGGGACCAGT TCTCGCCAGA TGATTTTTTG GGTCGGACGG AGATCCGCGT GGCCGACATC 
AAGAAGGACC AGGGCTCCAA GGGTCCAGTT ACGAAGTGTC TCCTGCTGCA CGAAGTTCCC 
ACGGGAGAGA TCGTGGTCCG CTTGGACCTG CAGTTGTTCG ATGAGCCGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITSN1 ( XM_025281327.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025137112.1 Bubalus bubalis intersectin 1 (ITSN1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OBu06695
Clone ID Related Accession (Same CDS sequence)	XM_025281327.1 , XM_025281333.1 , XM_006048226.1 , XM_025281317.1 , XM_025281323.1
Accession Version	XM_025281327.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5166bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	intersectin-1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037545.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCAGT TTCCCACACC TTTTGGTGGC AGCCTGGATA TTTGGGCCAT AACTGTAGAG 
GAAAGAGCAA AGCATGATCA ACAGTTCCAT AGTTTAAAGC CAATATCTGG ATTTATTACT 
GGTGATCAAG CTAGAAACTT TTTTTTTCAA TCTGGGTTAC CTCAGCCTGT TTTAGCACAG 
ATATGGGCGC TCGCTGATAT GAATAATGAT GGAAGGATGG ATCAAGTGGA GTTTTCCATT 
GCTATGAAAC TCATCAAGCT GAAGCTCCAA GGATATCAGC TCCCCTCTGC ACTTCCCCCT 
GTCATGAAAC AGCAACCAGT TGCTATTTCT AGTGCACCAG CGTTTGGTAT GGGAGGTATT 
GCCAGCATGC CGCCGCTGAC CGCTGTTGCT CCTGTGCCAA TGGGCTCCAT TCCCGTTGTT 
GGCATGTCTC CCCCCCTAGT ATCATCTGTT CCTGCAGCAG GGGTGCCCCC CCTGGCCAAC 
GGGGCGCCCC CGGTCATACA GCCTCTGCCG GCATTTGCTC ATCCTGCTGC CACATTGCCA 
AAGAGTTCTT CCTTTAGTAG ATCTGGCCCA GGGTCGCAGT TAAACACAAA ATTACAGAAG 
GCACAATCAT TTGATGTGGC CAGCGTCCCT CCTACAGCTG AGTGGGCTGT TCCTCAGTCA 
TCCAGGCTGA AATACAGACA GCTCTTCAAT AGTCATGACA AAACCATGAG TGGACACCTA 
ACAGGTCCCC AAGCAAGAAC TATTCTTATG CAATCAAGTT TACCACAGGC TCAGCTGGCT 
TCAATATGGA ATCTTTCTGA CATTGATCAA GATGGAAAAC TCACAGCAGA AGAATTTATC 
CTGGCTATGC ACCTCATTGA TGTAGCTATG TCTGGTCAAC CATTGCCACC GGTCCTGCCC 
CCAGAATACA TTCCACCTTC CTTTAGAAGA GTTCGATCTG GTAGTGGTAT ATCTGTCATA 
ACCTCAACTT CGGTGGATCA GAGGTTGCCA GAGGAACCAG TTTTGGAAGA TGAACAGCAA 
CAACTAGAAA AGAAACTACC TGTAACCTTT GAAGATAAGA AGCGGGAGAA CTTTGAACGG 
GGCAACCTGG AGCTGGAGAA GCGGAGACAA GCGCTCTTGG AGCAGCAGCG TAAAGAGCAG 
GAGCGCCTGG CCCAGCTGGA GCGGGCAGAA CAGGAGAGGA AGGAGCGGGA GCGCCAGGAG 
CAGGAGCGCA AAAGACAACT GGAGCTGGAA AAGCAGCTAG AGAAGCAGCG GGAGCTGGAG 
CGGCAGAGGG AGGAGGAGCG AAGGAAGGAA ATCGAGAGGC GGGAGGCTGC GAAACGGGAG 
CTTGAAAGGC AAAGACAGCT TGAATGGGAA CGGAATCGAA GACAAGAACT ATTAAATCAA 
AGAAACAAAG AACAAGAAGA CATAGTTGTA CTGAAAGCAA AGAAAAAGAC TTTGGAATTT 
GAATTAGAAG CTCTAAATGA TAAAAAACAT CAATTAGAAG GAAAACTTCA GGATATCAGA 
TGTCGACTGA CAACCCAAAG GCAAGAAATT GAGAGCACAA ACAAATCTAG AGAGTTGAGA 
ATTGCAGAAA TCACCCACCT ACAACAGCAA TTACAGGAGT CCCAGCAAAT GCTTGGAAGA 
CTTATTCCAG AAAAACAGAT ACTCAATGAC CAATTGAAAC AAGTTCAGCA AAACAGTTTG 
CACAGGGATT CACTTCTCAC ACTCAAAAGA GCCTTAGAAG CGAAAGAACT GGCTCGGCAG 
CAGCTACGAG ACCAACTGGA TGAAGTGGAG AAAGAAACTC GGTCAAAATT ACAGGAGATT 
GATATTTTCA ACAATCAGCT GAAGGAACTT AGAGAAATAC ATAATAAGCA GCAACTCCAG 
AAACAGAAGT CCATAGAGGC TGAACGACTG AAACAGAAGG AACAAGAACG AAAGATCCTG 
GAATTAGAAA AACAAAAAGA AGAAGCCCAA AGACGAGCTC AGGAAAGGGA CCAGCAGTGG 
CTGGAGCGCG TGCATCAGGA GGATGAGCAG CAGCGGCCAC GAAAACCCCA CGAGGAGGAG 
AAGCTCAAAA GGGAAGAGAG CGTCAAAAAG AAGGACAGTG AGGAGAAGGG CAAACCGGAA 
ATGCAAGACA AGCTGAGTCG GCTTTTCCAC CAGCATCAAG AACCAGCTAA GCCCGCTGTC 
CAGGCTCCCT GGTCCACTGC AGAAAAAGGC CCACTTACGA TTTCTGCACA GGAGAATGTA 
AAAGTGGTGT ATTACCGAGC ACTTTATCCC TTTGAGTCCA GGAGTCACGA TGAAATCACG 
ATCCAGCCGG GAGATATAGT CATGGTTAAA GGGGAATGGG TGGACGAAAG CCAAACTGGA 
GAACCGGGAT GGCTTGGAGG AGAATTAAAA GGAAAGACGG GATGGTTCCC TGCAAACTAC 
GCAGAGAAAA TCCCAGAAAA CGAGGTTCCC GCCCCAGTCA AACCAGTGAC TGAGGCGGCC 
TCCACCCCTA CACCCAAAGT GGCTGTGCGT GAGACCCCCG CCCCTTCATC TGCAACCCCT 
GCAGAGCCCT CCGAGACCCC CAACAACTGG GCCGACTTCA GCTCCACGTG GCCCACCAGC 
ACGAATGAGA AACCAGAAAC CGACAGCTGG GACGCATGGG CAACCCAGCC CTCCCTCACC 
GTGCCAAGCG CCGGGCAGCT GAGGCAGAGG TCGGCCTTCA CTCCAGCCAC GGCCACGGGC 
TCATCTCCGT CTCCTGTTCT AGGCCAGGGT GAAAAGGTGG AGGGGCTACA GGCGCAAGCC 
CTGTATCCGT GGAGAGCCAA GAAAGACAAC CATTTAAATT TTAACAAAAA CGATGTCATC 
ACCGTACTGG AGCAGCAAGA CATGTGGTGG TTTGGAGAAG TTCAAGGCCA GAAGGGTTGG 
TTCCCCAAGT CCTACGTGAA ACTCATTTCA GGGCCCATAA GGAAATCGAC AAGCATGGAT 
TCTGGTTCCT CAGAAAGTCC TGCAAGTCTG AAGAGGGTAG CTTCCCCAGC AGCCAAGCCA 
GCTGTGTCGG GAGAAGAATT TATTGCCATG TACACTTACG AGAGTTCTGA GCAAGGCGAT 
TTAACCTTTC AGCAAGGGGA TGTGATTTTG GTTACCAAGA AAGATGGTGA CTGGTGGACA 
GGAACAGTGG GCGACAAGTC CGGCGTCTTC CCTTCTAACT ATGTGAGGCT TAAAGATTCA 
GAGGGCTCTG GAACTGCTGG GAAAACAGGG AGTTTAGGAA AAAAACCTGA AATCGCACAG 
GTCATTGCCT CCTACACAGC TACAGGTCCT GAACAGCTTA CCCTGGCCCC CGGTCAGCTG 
ATCCTGATCC GGAAAAAGAA CCCAGGTGGA TGGTGGGAAG GAGAGCTACA GGCACGTGGG 
AAAAAGCGCC AGATAGGCTG GTTTCCTGCT AATTACGTCA AACTTTTAAG CCCTGGGACG 
AGCAAAATCA CTCCAACAGA CCCACCAAAG CCAACAGCTT TCCCAACAGT GTGCCAGGTG 
ATCGGGATGT ACGACTACAC CGCGCAGAAC GACGACGAGC TGGCCTTCAG CAAGGGCCAG 
GTCATCAATG TCCTCAACAA GGAGGACCCC GACTGGTGGA AGGGTGAAGT CCACGGGCAG 
GTGGGGCTCT TCCCGTCCAA CTACGTGAAG CTGACCACGG ACACGGACCC CAGCCAGCAA 
TGGTGTTCAG ACTTACACCT CTTGGATATG CTGACCCCAA CTGAAAGAAA GCGCCAGGGG 
TACATCCACG AGCTCATCGT CACAGAGGAG AACTATGTGA ATGACCTGCA GCTGGTCACA 
GAGATCTTTC AGAAACCCCT GATGGAGTCT GAGCTGCTGA CAGAAAAAGA GGGTGCTATG 
ATTTTTGTTA ACTGGAAGGA GCTGATTATG TGTAATATCA AACTACTGAA AGCGCTGAGG 
GTCCGCAAGA AGATGTCTGG GGAGAAGATG CCCGTGAAGA TGATCGGAGA CATCCTGACG 
GCCCAGCTGC CACACATGCA GCCCTACATC CGCTTCTGCA GCTGCCAGCT CAACGGGGCC 
GCCTTGATCC AGCAGAAGAC TGACGAGGCC CCAGACTTCA AGGAGTTTGT TAAAAGGCTG 
GCCATGGACC CACGCTGTAA GGGAATGCCC CTGTCTAGTT TTATACTGAA GCCTATGCAA 
CGAGTAACGA GATACCCACT GATCATTAAA AATATCCTGG AAAACACTCC CGAAAACCAC 
CCAGACCACA GCCACCTGAA GCACGCCCTG GAGAAGGCAG AGGAGCTGTG CTCCCAGGTG 
AACGAAGGGG TGCGGGAGAA GGAGAACTCG GACCGACTGG AGTGGATCCA GGCCCACGTA 
CTGTGTGAAG GCCTGTCAGA GCAACTTGTG TTCAATTCCG TGACCAACTG CCTGGGGCCG 
CGCAAGTTCC TGCACAGTGG GAAGCTCTAC AAGGCCAAGA GCAACAAGGA GCTGTATGGC 
TTCCTCTTCA ATGACTTCCT GCTGCTGACC CAAATCATCA AGCCCCTGGG CTCCTCAGGC 
ACCGACAAAG TCTTCAGCCC CAAATCAAAC CTGCAGTATA AAATGTACAA AACACCTATC 
TTCCTAAATG AGGTTCTAGT GAAGTTGCCC ACTGACCCTT CTGGGGATGA ACCCATCTTC 
CACATTTCCC ACATCGACCG AGTCTACACC CTCCGAGCAG AGAGCATAAA CGAAAGGACT 
GCTTGGGTGC AGAAAATCAA AGCAGCTTCT GAACTCTACA TAGAGACAGA GAAGAAGAAG 
CGGGAGAAGG CGTATCTGGT CCGCTCCCAA AGAGCCACAG GCATCGGAAG GTTGATGGTG 
AACGTGGTTG AAGGCATTGA GCTGAAACCC TGTCGCTCCC ATGGGAAGAG TAACCCATAC 
TGCGAGGTGA CCATGGGTTC CCAGTGTCAC ATCACCAAGA CGATCCAGGA CACTCTGAAT 
CCCAAGTGGA ATTCCAACTG CCAGTTCTTC ATCAGAGACT TGGAGCAAGA GGTGCTGTGC 
ATCACCGTGT TTGAGAGGGA CCAGTTCTCG CCAGATGATT TTTTGGGTCG GACGGAGATC 
CGCGTGGCCG ACATCAAGAA GGACCAGGGC TCCAAGGGTC CAGTTACGAA GTGTCTCCTG 
CTGCACGAAG TTCCCACGGG AGAGATCGTG GTCCGCTTGG ACCTGCAGTT GTTCGATGAG 
CCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025137112.1
CDS	377..5542
Translation	MAQFPTPFGGSLDIWAITVEERAKHDQQFHSLKPISGFITGDQARNFFFQSGLPQPVLAQIWALADMNNDGRMDQVEFSIAMKLIKLKLQGYQLPSALPPVMKQQPVAISSAPAFGMGGIASMPPLTAVAPVPMGSIPVVGMSPPLVSSVPAAGVPPLANGAPPVIQPLPAFAHPAATLPKSSSFSRSGPGSQLNTKLQKAQSFDVASVPPTAEWAVPQSSRLKYRQLFNSHDKTMSGHLTGPQARTILMQSSLPQAQLASIWNLSDIDQDGKLTAEEFILAMHLIDVAMSGQPLPPVLPPEYIPPSFRRVRSGSGISVITSTSVDQRLPEEPVLEDEQQQLEKKLPVTFEDKKRENFERGNLELEKRRQALLEQQRKEQERLAQLERAEQERKERERQEQERKRQLELEKQLEKQRELERQREEERRKEIERREAAKRELERQRQLEWERNRRQELLNQRNKEQEDIVVLKAKKKTLEFELEALNDKKHQLEGKLQDIRCRLTTQRQEIESTNKSRELRIAEITHLQQQLQESQQMLGRLIPEKQILNDQLKQVQQNSLHRDSLLTLKRALEAKELARQQLRDQLDEVEKETRSKLQEIDIFNNQLKELREIHNKQQLQKQKSIEAERLKQKEQERKILELEKQKEEAQRRAQERDQQWLERVHQEDEQQRPRKPHEEEKLKREESVKKKDSEEKGKPEMQDKLSRLFHQHQEPAKPAVQAPWSTAEKGPLTISAQENVKVVYYRALYPFESRSHDEITIQPGDIVMVKGEWVDESQTGEPGWLGGELKGKTGWFPANYAEKIPENEVPAPVKPVTEAASTPTPKVAVRETPAPSSATPAEPSETPNNWADFSSTWPTSTNEKPETDSWDAWATQPSLTVPSAGQLRQRSAFTPATATGSSPSPVLGQGEKVEGLQAQALYPWRAKKDNHLNFNKNDVITVLEQQDMWWFGEVQGQKGWFPKSYVKLISGPIRKSTSMDSGSSESPASLKRVASPAAKPAVSGEEFIAMYTYESSEQGDLTFQQGDVILVTKKDGDWWTGTVGDKSGVFPSNYVRLKDSEGSGTAGKTGSLGKKPEIAQVIASYTATGPEQLTLAPGQLILIRKKNPGGWWEGELQARGKKRQIGWFPANYVKLLSPGTSKITPTDPPKPTAFPTVCQVIGMYDYTAQNDDELAFSKGQVINVLNKEDPDWWKGEVHGQVGLFPSNYVKLTTDTDPSQQWCSDLHLLDMLTPTERKRQGYIHELIVTEENYVNDLQLVTEIFQKPLMESELLTEKEGAMIFVNWKELIMCNIKLLKALRVRKKMSGEKMPVKMIGDILTAQLPHMQPYIRFCSCQLNGAALIQQKTDEAPDFKEFVKRLAMDPRCKGMPLSSFILKPMQRVTRYPLIIKNILENTPENHPDHSHLKHALEKAEELCSQVNEGVREKENSDRLEWIQAHVLCEGLSEQLVFNSVTNCLGPRKFLHSGKLYKAKSNKELYGFLFNDFLLLTQIIKPLGSSGTDKVFSPKSNLQYKMYKTPIFLNEVLVKLPTDPSGDEPIFHISHIDRVYTLRAESINERTAWVQKIKAASELYIETEKKKREKAYLVRSQRATGIGRLMVNVVEGIELKPCRSHGKSNPYCEVTMGSQCHITKTIQDTLNPKWNSNCQFFIRDLEQEVLCITVFERDQFSPDDFLGRTEIRVADIKKDQGSKGPVTKCLLLHEVPTGEIVVRLDLQLFDEP

Target ORF information:

RefSeq Version	XM_025281327.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis intersectin 1 (ITSN1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025281327.1

ORF Insert Sequence:

ATGGCTCAGT TTCCCACACC TTTTGGTGGC AGCCTGGATA TTTGGGCCAT AACTGTAGAG 
GAAAGAGCAA AGCATGATCA ACAGTTCCAT AGTTTAAAGC CAATATCTGG ATTTATTACT 
GGTGATCAAG CTAGAAACTT TTTTTTTCAA TCTGGGTTAC CTCAGCCTGT TTTAGCACAG 
ATATGGGCGC TCGCTGATAT GAATAATGAT GGAAGGATGG ATCAAGTGGA GTTTTCCATT 
GCTATGAAAC TCATCAAGCT GAAGCTCCAA GGATATCAGC TCCCCTCTGC ACTTCCCCCT 
GTCATGAAAC AGCAACCAGT TGCTATTTCT AGTGCACCAG CGTTTGGTAT GGGAGGTATT 
GCCAGCATGC CGCCGCTGAC CGCTGTTGCT CCTGTGCCAA TGGGCTCCAT TCCCGTTGTT 
GGCATGTCTC CCCCCCTAGT ATCATCTGTT CCTGCAGCAG GGGTGCCCCC CCTGGCCAAC 
GGGGCGCCCC CGGTCATACA GCCTCTGCCG GCATTTGCTC ATCCTGCTGC CACATTGCCA 
AAGAGTTCTT CCTTTAGTAG ATCTGGCCCA GGGTCGCAGT TAAACACAAA ATTACAGAAG 
GCACAATCAT TTGATGTGGC CAGCGTCCCT CCTACAGCTG AGTGGGCTGT TCCTCAGTCA 
TCCAGGCTGA AATACAGACA GCTCTTCAAT AGTCATGACA AAACCATGAG TGGACACCTA 
ACAGGTCCCC AAGCAAGAAC TATTCTTATG CAATCAAGTT TACCACAGGC TCAGCTGGCT 
TCAATATGGA ATCTTTCTGA CATTGATCAA GATGGAAAAC TCACAGCAGA AGAATTTATC 
CTGGCTATGC ACCTCATTGA TGTAGCTATG TCTGGTCAAC CATTGCCACC GGTCCTGCCC 
CCAGAATACA TTCCACCTTC CTTTAGAAGA GTTCGATCTG GTAGTGGTAT ATCTGTCATA 
ACCTCAACTT CGGTGGATCA GAGGTTGCCA GAGGAACCAG TTTTGGAAGA TGAACAGCAA 
CAACTAGAAA AGAAACTACC TGTAACCTTT GAAGATAAGA AGCGGGAGAA CTTTGAACGG 
GGCAACCTGG AGCTGGAGAA GCGGAGACAA GCGCTCTTGG AGCAGCAGCG TAAAGAGCAG 
GAGCGCCTGG CCCAGCTGGA GCGGGCAGAA CAGGAGAGGA AGGAGCGGGA GCGCCAGGAG 
CAGGAGCGCA AAAGACAACT GGAGCTGGAA AAGCAGCTAG AGAAGCAGCG GGAGCTGGAG 
CGGCAGAGGG AGGAGGAGCG AAGGAAGGAA ATCGAGAGGC GGGAGGCTGC GAAACGGGAG 
CTTGAAAGGC AAAGACAGCT TGAATGGGAA CGGAATCGAA GACAAGAACT ATTAAATCAA 
AGAAACAAAG AACAAGAAGA CATAGTTGTA CTGAAAGCAA AGAAAAAGAC TTTGGAATTT 
GAATTAGAAG CTCTAAATGA TAAAAAACAT CAATTAGAAG GAAAACTTCA GGATATCAGA 
TGTCGACTGA CAACCCAAAG GCAAGAAATT GAGAGCACAA ACAAATCTAG AGAGTTGAGA 
ATTGCAGAAA TCACCCACCT ACAACAGCAA TTACAGGAGT CCCAGCAAAT GCTTGGAAGA 
CTTATTCCAG AAAAACAGAT ACTCAATGAC CAATTGAAAC AAGTTCAGCA AAACAGTTTG 
CACAGGGATT CACTTCTCAC ACTCAAAAGA GCCTTAGAAG CGAAAGAACT GGCTCGGCAG 
CAGCTACGAG ACCAACTGGA TGAAGTGGAG AAAGAAACTC GGTCAAAATT ACAGGAGATT 
GATATTTTCA ACAATCAGCT GAAGGAACTT AGAGAAATAC ATAATAAGCA GCAACTCCAG 
AAACAGAAGT CCATAGAGGC TGAACGACTG AAACAGAAGG AACAAGAACG AAAGATCCTG 
GAATTAGAAA AACAAAAAGA AGAAGCCCAA AGACGAGCTC AGGAAAGGGA CCAGCAGTGG 
CTGGAGCGCG TGCATCAGGA GGATGAGCAG CAGCGGCCAC GAAAACCCCA CGAGGAGGAG 
AAGCTCAAAA GGGAAGAGAG CGTCAAAAAG AAGGACAGTG AGGAGAAGGG CAAACCGGAA 
ATGCAAGACA AGCTGAGTCG GCTTTTCCAC CAGCATCAAG AACCAGCTAA GCCCGCTGTC 
CAGGCTCCCT GGTCCACTGC AGAAAAAGGC CCACTTACGA TTTCTGCACA GGAGAATGTA 
AAAGTGGTGT ATTACCGAGC ACTTTATCCC TTTGAGTCCA GGAGTCACGA TGAAATCACG 
ATCCAGCCGG GAGATATAGT CATGGTTAAA GGGGAATGGG TGGACGAAAG CCAAACTGGA 
GAACCGGGAT GGCTTGGAGG AGAATTAAAA GGAAAGACGG GATGGTTCCC TGCAAACTAC 
GCAGAGAAAA TCCCAGAAAA CGAGGTTCCC GCCCCAGTCA AACCAGTGAC TGAGGCGGCC 
TCCACCCCTA CACCCAAAGT GGCTGTGCGT GAGACCCCCG CCCCTTCATC TGCAACCCCT 
GCAGAGCCCT CCGAGACCCC CAACAACTGG GCCGACTTCA GCTCCACGTG GCCCACCAGC 
ACGAATGAGA AACCAGAAAC CGACAGCTGG GACGCATGGG CAACCCAGCC CTCCCTCACC 
GTGCCAAGCG CCGGGCAGCT GAGGCAGAGG TCGGCCTTCA CTCCAGCCAC GGCCACGGGC 
TCATCTCCGT CTCCTGTTCT AGGCCAGGGT GAAAAGGTGG AGGGGCTACA GGCGCAAGCC 
CTGTATCCGT GGAGAGCCAA GAAAGACAAC CATTTAAATT TTAACAAAAA CGATGTCATC 
ACCGTACTGG AGCAGCAAGA CATGTGGTGG TTTGGAGAAG TTCAAGGCCA GAAGGGTTGG 
TTCCCCAAGT CCTACGTGAA ACTCATTTCA GGGCCCATAA GGAAATCGAC AAGCATGGAT 
TCTGGTTCCT CAGAAAGTCC TGCAAGTCTG AAGAGGGTAG CTTCCCCAGC AGCCAAGCCA 
GCTGTGTCGG GAGAAGAATT TATTGCCATG TACACTTACG AGAGTTCTGA GCAAGGCGAT 
TTAACCTTTC AGCAAGGGGA TGTGATTTTG GTTACCAAGA AAGATGGTGA CTGGTGGACA 
GGAACAGTGG GCGACAAGTC CGGCGTCTTC CCTTCTAACT ATGTGAGGCT TAAAGATTCA 
GAGGGCTCTG GAACTGCTGG GAAAACAGGG AGTTTAGGAA AAAAACCTGA AATCGCACAG 
GTCATTGCCT CCTACACAGC TACAGGTCCT GAACAGCTTA CCCTGGCCCC CGGTCAGCTG 
ATCCTGATCC GGAAAAAGAA CCCAGGTGGA TGGTGGGAAG GAGAGCTACA GGCACGTGGG 
AAAAAGCGCC AGATAGGCTG GTTTCCTGCT AATTACGTCA AACTTTTAAG CCCTGGGACG 
AGCAAAATCA CTCCAACAGA CCCACCAAAG CCAACAGCTT TCCCAACAGT GTGCCAGGTG 
ATCGGGATGT ACGACTACAC CGCGCAGAAC GACGACGAGC TGGCCTTCAG CAAGGGCCAG 
GTCATCAATG TCCTCAACAA GGAGGACCCC GACTGGTGGA AGGGTGAAGT CCACGGGCAG 
GTGGGGCTCT TCCCGTCCAA CTACGTGAAG CTGACCACGG ACACGGACCC CAGCCAGCAA 
TGGTGTTCAG ACTTACACCT CTTGGATATG CTGACCCCAA CTGAAAGAAA GCGCCAGGGG 
TACATCCACG AGCTCATCGT CACAGAGGAG AACTATGTGA ATGACCTGCA GCTGGTCACA 
GAGATCTTTC AGAAACCCCT GATGGAGTCT GAGCTGCTGA CAGAAAAAGA GGGTGCTATG 
ATTTTTGTTA ACTGGAAGGA GCTGATTATG TGTAATATCA AACTACTGAA AGCGCTGAGG 
GTCCGCAAGA AGATGTCTGG GGAGAAGATG CCCGTGAAGA TGATCGGAGA CATCCTGACG 
GCCCAGCTGC CACACATGCA GCCCTACATC CGCTTCTGCA GCTGCCAGCT CAACGGGGCC 
GCCTTGATCC AGCAGAAGAC TGACGAGGCC CCAGACTTCA AGGAGTTTGT TAAAAGGCTG 
GCCATGGACC CACGCTGTAA GGGAATGCCC CTGTCTAGTT TTATACTGAA GCCTATGCAA 
CGAGTAACGA GATACCCACT GATCATTAAA AATATCCTGG AAAACACTCC CGAAAACCAC 
CCAGACCACA GCCACCTGAA GCACGCCCTG GAGAAGGCAG AGGAGCTGTG CTCCCAGGTG 
AACGAAGGGG TGCGGGAGAA GGAGAACTCG GACCGACTGG AGTGGATCCA GGCCCACGTA 
CTGTGTGAAG GCCTGTCAGA GCAACTTGTG TTCAATTCCG TGACCAACTG CCTGGGGCCG 
CGCAAGTTCC TGCACAGTGG GAAGCTCTAC AAGGCCAAGA GCAACAAGGA GCTGTATGGC 
TTCCTCTTCA ATGACTTCCT GCTGCTGACC CAAATCATCA AGCCCCTGGG CTCCTCAGGC 
ACCGACAAAG TCTTCAGCCC CAAATCAAAC CTGCAGTATA AAATGTACAA AACACCTATC 
TTCCTAAATG AGGTTCTAGT GAAGTTGCCC ACTGACCCTT CTGGGGATGA ACCCATCTTC 
CACATTTCCC ACATCGACCG AGTCTACACC CTCCGAGCAG AGAGCATAAA CGAAAGGACT 
GCTTGGGTGC AGAAAATCAA AGCAGCTTCT GAACTCTACA TAGAGACAGA GAAGAAGAAG 
CGGGAGAAGG CGTATCTGGT CCGCTCCCAA AGAGCCACAG GCATCGGAAG GTTGATGGTG 
AACGTGGTTG AAGGCATTGA GCTGAAACCC TGTCGCTCCC ATGGGAAGAG TAACCCATAC 
TGCGAGGTGA CCATGGGTTC CCAGTGTCAC ATCACCAAGA CGATCCAGGA CACTCTGAAT 
CCCAAGTGGA ATTCCAACTG CCAGTTCTTC ATCAGAGACT TGGAGCAAGA GGTGCTGTGC 
ATCACCGTGT TTGAGAGGGA CCAGTTCTCG CCAGATGATT TTTTGGGTCG GACGGAGATC 
CGCGTGGCCG ACATCAAGAA GGACCAGGGC TCCAAGGGTC CAGTTACGAA GTGTCTCCTG 
CTGCACGAAG TTCCCACGGG AGAGATCGTG GTCCGCTTGG ACCTGCAGTT GTTCGATGAG 
CCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITSN1 ( XM_025281333.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025137118.1 Bubalus bubalis intersectin 1 (ITSN1), transcript variant X6, mRNA.

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CloneID	OBu06695
Clone ID Related Accession (Same CDS sequence)	XM_025281327.1 , XM_025281333.1 , XM_006048226.1 , XM_025281317.1 , XM_025281323.1
Accession Version	XM_025281333.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5166bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	intersectin-1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037545.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCAGT TTCCCACACC TTTTGGTGGC AGCCTGGATA TTTGGGCCAT AACTGTAGAG 
GAAAGAGCAA AGCATGATCA ACAGTTCCAT AGTTTAAAGC CAATATCTGG ATTTATTACT 
GGTGATCAAG CTAGAAACTT TTTTTTTCAA TCTGGGTTAC CTCAGCCTGT TTTAGCACAG 
ATATGGGCGC TCGCTGATAT GAATAATGAT GGAAGGATGG ATCAAGTGGA GTTTTCCATT 
GCTATGAAAC TCATCAAGCT GAAGCTCCAA GGATATCAGC TCCCCTCTGC ACTTCCCCCT 
GTCATGAAAC AGCAACCAGT TGCTATTTCT AGTGCACCAG CGTTTGGTAT GGGAGGTATT 
GCCAGCATGC CGCCGCTGAC CGCTGTTGCT CCTGTGCCAA TGGGCTCCAT TCCCGTTGTT 
GGCATGTCTC CCCCCCTAGT ATCATCTGTT CCTGCAGCAG GGGTGCCCCC CCTGGCCAAC 
GGGGCGCCCC CGGTCATACA GCCTCTGCCG GCATTTGCTC ATCCTGCTGC CACATTGCCA 
AAGAGTTCTT CCTTTAGTAG ATCTGGCCCA GGGTCGCAGT TAAACACAAA ATTACAGAAG 
GCACAATCAT TTGATGTGGC CAGCGTCCCT CCTACAGCTG AGTGGGCTGT TCCTCAGTCA 
TCCAGGCTGA AATACAGACA GCTCTTCAAT AGTCATGACA AAACCATGAG TGGACACCTA 
ACAGGTCCCC AAGCAAGAAC TATTCTTATG CAATCAAGTT TACCACAGGC TCAGCTGGCT 
TCAATATGGA ATCTTTCTGA CATTGATCAA GATGGAAAAC TCACAGCAGA AGAATTTATC 
CTGGCTATGC ACCTCATTGA TGTAGCTATG TCTGGTCAAC CATTGCCACC GGTCCTGCCC 
CCAGAATACA TTCCACCTTC CTTTAGAAGA GTTCGATCTG GTAGTGGTAT ATCTGTCATA 
ACCTCAACTT CGGTGGATCA GAGGTTGCCA GAGGAACCAG TTTTGGAAGA TGAACAGCAA 
CAACTAGAAA AGAAACTACC TGTAACCTTT GAAGATAAGA AGCGGGAGAA CTTTGAACGG 
GGCAACCTGG AGCTGGAGAA GCGGAGACAA GCGCTCTTGG AGCAGCAGCG TAAAGAGCAG 
GAGCGCCTGG CCCAGCTGGA GCGGGCAGAA CAGGAGAGGA AGGAGCGGGA GCGCCAGGAG 
CAGGAGCGCA AAAGACAACT GGAGCTGGAA AAGCAGCTAG AGAAGCAGCG GGAGCTGGAG 
CGGCAGAGGG AGGAGGAGCG AAGGAAGGAA ATCGAGAGGC GGGAGGCTGC GAAACGGGAG 
CTTGAAAGGC AAAGACAGCT TGAATGGGAA CGGAATCGAA GACAAGAACT ATTAAATCAA 
AGAAACAAAG AACAAGAAGA CATAGTTGTA CTGAAAGCAA AGAAAAAGAC TTTGGAATTT 
GAATTAGAAG CTCTAAATGA TAAAAAACAT CAATTAGAAG GAAAACTTCA GGATATCAGA 
TGTCGACTGA CAACCCAAAG GCAAGAAATT GAGAGCACAA ACAAATCTAG AGAGTTGAGA 
ATTGCAGAAA TCACCCACCT ACAACAGCAA TTACAGGAGT CCCAGCAAAT GCTTGGAAGA 
CTTATTCCAG AAAAACAGAT ACTCAATGAC CAATTGAAAC AAGTTCAGCA AAACAGTTTG 
CACAGGGATT CACTTCTCAC ACTCAAAAGA GCCTTAGAAG CGAAAGAACT GGCTCGGCAG 
CAGCTACGAG ACCAACTGGA TGAAGTGGAG AAAGAAACTC GGTCAAAATT ACAGGAGATT 
GATATTTTCA ACAATCAGCT GAAGGAACTT AGAGAAATAC ATAATAAGCA GCAACTCCAG 
AAACAGAAGT CCATAGAGGC TGAACGACTG AAACAGAAGG AACAAGAACG AAAGATCCTG 
GAATTAGAAA AACAAAAAGA AGAAGCCCAA AGACGAGCTC AGGAAAGGGA CCAGCAGTGG 
CTGGAGCGCG TGCATCAGGA GGATGAGCAG CAGCGGCCAC GAAAACCCCA CGAGGAGGAG 
AAGCTCAAAA GGGAAGAGAG CGTCAAAAAG AAGGACAGTG AGGAGAAGGG CAAACCGGAA 
ATGCAAGACA AGCTGAGTCG GCTTTTCCAC CAGCATCAAG AACCAGCTAA GCCCGCTGTC 
CAGGCTCCCT GGTCCACTGC AGAAAAAGGC CCACTTACGA TTTCTGCACA GGAGAATGTA 
AAAGTGGTGT ATTACCGAGC ACTTTATCCC TTTGAGTCCA GGAGTCACGA TGAAATCACG 
ATCCAGCCGG GAGATATAGT CATGGTTAAA GGGGAATGGG TGGACGAAAG CCAAACTGGA 
GAACCGGGAT GGCTTGGAGG AGAATTAAAA GGAAAGACGG GATGGTTCCC TGCAAACTAC 
GCAGAGAAAA TCCCAGAAAA CGAGGTTCCC GCCCCAGTCA AACCAGTGAC TGAGGCGGCC 
TCCACCCCTA CACCCAAAGT GGCTGTGCGT GAGACCCCCG CCCCTTCATC TGCAACCCCT 
GCAGAGCCCT CCGAGACCCC CAACAACTGG GCCGACTTCA GCTCCACGTG GCCCACCAGC 
ACGAATGAGA AACCAGAAAC CGACAGCTGG GACGCATGGG CAACCCAGCC CTCCCTCACC 
GTGCCAAGCG CCGGGCAGCT GAGGCAGAGG TCGGCCTTCA CTCCAGCCAC GGCCACGGGC 
TCATCTCCGT CTCCTGTTCT AGGCCAGGGT GAAAAGGTGG AGGGGCTACA GGCGCAAGCC 
CTGTATCCGT GGAGAGCCAA GAAAGACAAC CATTTAAATT TTAACAAAAA CGATGTCATC 
ACCGTACTGG AGCAGCAAGA CATGTGGTGG TTTGGAGAAG TTCAAGGCCA GAAGGGTTGG 
TTCCCCAAGT CCTACGTGAA ACTCATTTCA GGGCCCATAA GGAAATCGAC AAGCATGGAT 
TCTGGTTCCT CAGAAAGTCC TGCAAGTCTG AAGAGGGTAG CTTCCCCAGC AGCCAAGCCA 
GCTGTGTCGG GAGAAGAATT TATTGCCATG TACACTTACG AGAGTTCTGA GCAAGGCGAT 
TTAACCTTTC AGCAAGGGGA TGTGATTTTG GTTACCAAGA AAGATGGTGA CTGGTGGACA 
GGAACAGTGG GCGACAAGTC CGGCGTCTTC CCTTCTAACT ATGTGAGGCT TAAAGATTCA 
GAGGGCTCTG GAACTGCTGG GAAAACAGGG AGTTTAGGAA AAAAACCTGA AATCGCACAG 
GTCATTGCCT CCTACACAGC TACAGGTCCT GAACAGCTTA CCCTGGCCCC CGGTCAGCTG 
ATCCTGATCC GGAAAAAGAA CCCAGGTGGA TGGTGGGAAG GAGAGCTACA GGCACGTGGG 
AAAAAGCGCC AGATAGGCTG GTTTCCTGCT AATTACGTCA AACTTTTAAG CCCTGGGACG 
AGCAAAATCA CTCCAACAGA CCCACCAAAG CCAACAGCTT TCCCAACAGT GTGCCAGGTG 
ATCGGGATGT ACGACTACAC CGCGCAGAAC GACGACGAGC TGGCCTTCAG CAAGGGCCAG 
GTCATCAATG TCCTCAACAA GGAGGACCCC GACTGGTGGA AGGGTGAAGT CCACGGGCAG 
GTGGGGCTCT TCCCGTCCAA CTACGTGAAG CTGACCACGG ACACGGACCC CAGCCAGCAA 
TGGTGTTCAG ACTTACACCT CTTGGATATG CTGACCCCAA CTGAAAGAAA GCGCCAGGGG 
TACATCCACG AGCTCATCGT CACAGAGGAG AACTATGTGA ATGACCTGCA GCTGGTCACA 
GAGATCTTTC AGAAACCCCT GATGGAGTCT GAGCTGCTGA CAGAAAAAGA GGGTGCTATG 
ATTTTTGTTA ACTGGAAGGA GCTGATTATG TGTAATATCA AACTACTGAA AGCGCTGAGG 
GTCCGCAAGA AGATGTCTGG GGAGAAGATG CCCGTGAAGA TGATCGGAGA CATCCTGACG 
GCCCAGCTGC CACACATGCA GCCCTACATC CGCTTCTGCA GCTGCCAGCT CAACGGGGCC 
GCCTTGATCC AGCAGAAGAC TGACGAGGCC CCAGACTTCA AGGAGTTTGT TAAAAGGCTG 
GCCATGGACC CACGCTGTAA GGGAATGCCC CTGTCTAGTT TTATACTGAA GCCTATGCAA 
CGAGTAACGA GATACCCACT GATCATTAAA AATATCCTGG AAAACACTCC CGAAAACCAC 
CCAGACCACA GCCACCTGAA GCACGCCCTG GAGAAGGCAG AGGAGCTGTG CTCCCAGGTG 
AACGAAGGGG TGCGGGAGAA GGAGAACTCG GACCGACTGG AGTGGATCCA GGCCCACGTA 
CTGTGTGAAG GCCTGTCAGA GCAACTTGTG TTCAATTCCG TGACCAACTG CCTGGGGCCG 
CGCAAGTTCC TGCACAGTGG GAAGCTCTAC AAGGCCAAGA GCAACAAGGA GCTGTATGGC 
TTCCTCTTCA ATGACTTCCT GCTGCTGACC CAAATCATCA AGCCCCTGGG CTCCTCAGGC 
ACCGACAAAG TCTTCAGCCC CAAATCAAAC CTGCAGTATA AAATGTACAA AACACCTATC 
TTCCTAAATG AGGTTCTAGT GAAGTTGCCC ACTGACCCTT CTGGGGATGA ACCCATCTTC 
CACATTTCCC ACATCGACCG AGTCTACACC CTCCGAGCAG AGAGCATAAA CGAAAGGACT 
GCTTGGGTGC AGAAAATCAA AGCAGCTTCT GAACTCTACA TAGAGACAGA GAAGAAGAAG 
CGGGAGAAGG CGTATCTGGT CCGCTCCCAA AGAGCCACAG GCATCGGAAG GTTGATGGTG 
AACGTGGTTG AAGGCATTGA GCTGAAACCC TGTCGCTCCC ATGGGAAGAG TAACCCATAC 
TGCGAGGTGA CCATGGGTTC CCAGTGTCAC ATCACCAAGA CGATCCAGGA CACTCTGAAT 
CCCAAGTGGA ATTCCAACTG CCAGTTCTTC ATCAGAGACT TGGAGCAAGA GGTGCTGTGC 
ATCACCGTGT TTGAGAGGGA CCAGTTCTCG CCAGATGATT TTTTGGGTCG GACGGAGATC 
CGCGTGGCCG ACATCAAGAA GGACCAGGGC TCCAAGGGTC CAGTTACGAA GTGTCTCCTG 
CTGCACGAAG TTCCCACGGG AGAGATCGTG GTCCGCTTGG ACCTGCAGTT GTTCGATGAG 
CCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025137118.1
CDS	216..5381
Translation	MAQFPTPFGGSLDIWAITVEERAKHDQQFHSLKPISGFITGDQARNFFFQSGLPQPVLAQIWALADMNNDGRMDQVEFSIAMKLIKLKLQGYQLPSALPPVMKQQPVAISSAPAFGMGGIASMPPLTAVAPVPMGSIPVVGMSPPLVSSVPAAGVPPLANGAPPVIQPLPAFAHPAATLPKSSSFSRSGPGSQLNTKLQKAQSFDVASVPPTAEWAVPQSSRLKYRQLFNSHDKTMSGHLTGPQARTILMQSSLPQAQLASIWNLSDIDQDGKLTAEEFILAMHLIDVAMSGQPLPPVLPPEYIPPSFRRVRSGSGISVITSTSVDQRLPEEPVLEDEQQQLEKKLPVTFEDKKRENFERGNLELEKRRQALLEQQRKEQERLAQLERAEQERKERERQEQERKRQLELEKQLEKQRELERQREEERRKEIERREAAKRELERQRQLEWERNRRQELLNQRNKEQEDIVVLKAKKKTLEFELEALNDKKHQLEGKLQDIRCRLTTQRQEIESTNKSRELRIAEITHLQQQLQESQQMLGRLIPEKQILNDQLKQVQQNSLHRDSLLTLKRALEAKELARQQLRDQLDEVEKETRSKLQEIDIFNNQLKELREIHNKQQLQKQKSIEAERLKQKEQERKILELEKQKEEAQRRAQERDQQWLERVHQEDEQQRPRKPHEEEKLKREESVKKKDSEEKGKPEMQDKLSRLFHQHQEPAKPAVQAPWSTAEKGPLTISAQENVKVVYYRALYPFESRSHDEITIQPGDIVMVKGEWVDESQTGEPGWLGGELKGKTGWFPANYAEKIPENEVPAPVKPVTEAASTPTPKVAVRETPAPSSATPAEPSETPNNWADFSSTWPTSTNEKPETDSWDAWATQPSLTVPSAGQLRQRSAFTPATATGSSPSPVLGQGEKVEGLQAQALYPWRAKKDNHLNFNKNDVITVLEQQDMWWFGEVQGQKGWFPKSYVKLISGPIRKSTSMDSGSSESPASLKRVASPAAKPAVSGEEFIAMYTYESSEQGDLTFQQGDVILVTKKDGDWWTGTVGDKSGVFPSNYVRLKDSEGSGTAGKTGSLGKKPEIAQVIASYTATGPEQLTLAPGQLILIRKKNPGGWWEGELQARGKKRQIGWFPANYVKLLSPGTSKITPTDPPKPTAFPTVCQVIGMYDYTAQNDDELAFSKGQVINVLNKEDPDWWKGEVHGQVGLFPSNYVKLTTDTDPSQQWCSDLHLLDMLTPTERKRQGYIHELIVTEENYVNDLQLVTEIFQKPLMESELLTEKEGAMIFVNWKELIMCNIKLLKALRVRKKMSGEKMPVKMIGDILTAQLPHMQPYIRFCSCQLNGAALIQQKTDEAPDFKEFVKRLAMDPRCKGMPLSSFILKPMQRVTRYPLIIKNILENTPENHPDHSHLKHALEKAEELCSQVNEGVREKENSDRLEWIQAHVLCEGLSEQLVFNSVTNCLGPRKFLHSGKLYKAKSNKELYGFLFNDFLLLTQIIKPLGSSGTDKVFSPKSNLQYKMYKTPIFLNEVLVKLPTDPSGDEPIFHISHIDRVYTLRAESINERTAWVQKIKAASELYIETEKKKREKAYLVRSQRATGIGRLMVNVVEGIELKPCRSHGKSNPYCEVTMGSQCHITKTIQDTLNPKWNSNCQFFIRDLEQEVLCITVFERDQFSPDDFLGRTEIRVADIKKDQGSKGPVTKCLLLHEVPTGEIVVRLDLQLFDEP

Target ORF information:

RefSeq Version	XM_025281333.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis intersectin 1 (ITSN1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025281333.1

ORF Insert Sequence:

ATGGCTCAGT TTCCCACACC TTTTGGTGGC AGCCTGGATA TTTGGGCCAT AACTGTAGAG 
GAAAGAGCAA AGCATGATCA ACAGTTCCAT AGTTTAAAGC CAATATCTGG ATTTATTACT 
GGTGATCAAG CTAGAAACTT TTTTTTTCAA TCTGGGTTAC CTCAGCCTGT TTTAGCACAG 
ATATGGGCGC TCGCTGATAT GAATAATGAT GGAAGGATGG ATCAAGTGGA GTTTTCCATT 
GCTATGAAAC TCATCAAGCT GAAGCTCCAA GGATATCAGC TCCCCTCTGC ACTTCCCCCT 
GTCATGAAAC AGCAACCAGT TGCTATTTCT AGTGCACCAG CGTTTGGTAT GGGAGGTATT 
GCCAGCATGC CGCCGCTGAC CGCTGTTGCT CCTGTGCCAA TGGGCTCCAT TCCCGTTGTT 
GGCATGTCTC CCCCCCTAGT ATCATCTGTT CCTGCAGCAG GGGTGCCCCC CCTGGCCAAC 
GGGGCGCCCC CGGTCATACA GCCTCTGCCG GCATTTGCTC ATCCTGCTGC CACATTGCCA 
AAGAGTTCTT CCTTTAGTAG ATCTGGCCCA GGGTCGCAGT TAAACACAAA ATTACAGAAG 
GCACAATCAT TTGATGTGGC CAGCGTCCCT CCTACAGCTG AGTGGGCTGT TCCTCAGTCA 
TCCAGGCTGA AATACAGACA GCTCTTCAAT AGTCATGACA AAACCATGAG TGGACACCTA 
ACAGGTCCCC AAGCAAGAAC TATTCTTATG CAATCAAGTT TACCACAGGC TCAGCTGGCT 
TCAATATGGA ATCTTTCTGA CATTGATCAA GATGGAAAAC TCACAGCAGA AGAATTTATC 
CTGGCTATGC ACCTCATTGA TGTAGCTATG TCTGGTCAAC CATTGCCACC GGTCCTGCCC 
CCAGAATACA TTCCACCTTC CTTTAGAAGA GTTCGATCTG GTAGTGGTAT ATCTGTCATA 
ACCTCAACTT CGGTGGATCA GAGGTTGCCA GAGGAACCAG TTTTGGAAGA TGAACAGCAA 
CAACTAGAAA AGAAACTACC TGTAACCTTT GAAGATAAGA AGCGGGAGAA CTTTGAACGG 
GGCAACCTGG AGCTGGAGAA GCGGAGACAA GCGCTCTTGG AGCAGCAGCG TAAAGAGCAG 
GAGCGCCTGG CCCAGCTGGA GCGGGCAGAA CAGGAGAGGA AGGAGCGGGA GCGCCAGGAG 
CAGGAGCGCA AAAGACAACT GGAGCTGGAA AAGCAGCTAG AGAAGCAGCG GGAGCTGGAG 
CGGCAGAGGG AGGAGGAGCG AAGGAAGGAA ATCGAGAGGC GGGAGGCTGC GAAACGGGAG 
CTTGAAAGGC AAAGACAGCT TGAATGGGAA CGGAATCGAA GACAAGAACT ATTAAATCAA 
AGAAACAAAG AACAAGAAGA CATAGTTGTA CTGAAAGCAA AGAAAAAGAC TTTGGAATTT 
GAATTAGAAG CTCTAAATGA TAAAAAACAT CAATTAGAAG GAAAACTTCA GGATATCAGA 
TGTCGACTGA CAACCCAAAG GCAAGAAATT GAGAGCACAA ACAAATCTAG AGAGTTGAGA 
ATTGCAGAAA TCACCCACCT ACAACAGCAA TTACAGGAGT CCCAGCAAAT GCTTGGAAGA 
CTTATTCCAG AAAAACAGAT ACTCAATGAC CAATTGAAAC AAGTTCAGCA AAACAGTTTG 
CACAGGGATT CACTTCTCAC ACTCAAAAGA GCCTTAGAAG CGAAAGAACT GGCTCGGCAG 
CAGCTACGAG ACCAACTGGA TGAAGTGGAG AAAGAAACTC GGTCAAAATT ACAGGAGATT 
GATATTTTCA ACAATCAGCT GAAGGAACTT AGAGAAATAC ATAATAAGCA GCAACTCCAG 
AAACAGAAGT CCATAGAGGC TGAACGACTG AAACAGAAGG AACAAGAACG AAAGATCCTG 
GAATTAGAAA AACAAAAAGA AGAAGCCCAA AGACGAGCTC AGGAAAGGGA CCAGCAGTGG 
CTGGAGCGCG TGCATCAGGA GGATGAGCAG CAGCGGCCAC GAAAACCCCA CGAGGAGGAG 
AAGCTCAAAA GGGAAGAGAG CGTCAAAAAG AAGGACAGTG AGGAGAAGGG CAAACCGGAA 
ATGCAAGACA AGCTGAGTCG GCTTTTCCAC CAGCATCAAG AACCAGCTAA GCCCGCTGTC 
CAGGCTCCCT GGTCCACTGC AGAAAAAGGC CCACTTACGA TTTCTGCACA GGAGAATGTA 
AAAGTGGTGT ATTACCGAGC ACTTTATCCC TTTGAGTCCA GGAGTCACGA TGAAATCACG 
ATCCAGCCGG GAGATATAGT CATGGTTAAA GGGGAATGGG TGGACGAAAG CCAAACTGGA 
GAACCGGGAT GGCTTGGAGG AGAATTAAAA GGAAAGACGG GATGGTTCCC TGCAAACTAC 
GCAGAGAAAA TCCCAGAAAA CGAGGTTCCC GCCCCAGTCA AACCAGTGAC TGAGGCGGCC 
TCCACCCCTA CACCCAAAGT GGCTGTGCGT GAGACCCCCG CCCCTTCATC TGCAACCCCT 
GCAGAGCCCT CCGAGACCCC CAACAACTGG GCCGACTTCA GCTCCACGTG GCCCACCAGC 
ACGAATGAGA AACCAGAAAC CGACAGCTGG GACGCATGGG CAACCCAGCC CTCCCTCACC 
GTGCCAAGCG CCGGGCAGCT GAGGCAGAGG TCGGCCTTCA CTCCAGCCAC GGCCACGGGC 
TCATCTCCGT CTCCTGTTCT AGGCCAGGGT GAAAAGGTGG AGGGGCTACA GGCGCAAGCC 
CTGTATCCGT GGAGAGCCAA GAAAGACAAC CATTTAAATT TTAACAAAAA CGATGTCATC 
ACCGTACTGG AGCAGCAAGA CATGTGGTGG TTTGGAGAAG TTCAAGGCCA GAAGGGTTGG 
TTCCCCAAGT CCTACGTGAA ACTCATTTCA GGGCCCATAA GGAAATCGAC AAGCATGGAT 
TCTGGTTCCT CAGAAAGTCC TGCAAGTCTG AAGAGGGTAG CTTCCCCAGC AGCCAAGCCA 
GCTGTGTCGG GAGAAGAATT TATTGCCATG TACACTTACG AGAGTTCTGA GCAAGGCGAT 
TTAACCTTTC AGCAAGGGGA TGTGATTTTG GTTACCAAGA AAGATGGTGA CTGGTGGACA 
GGAACAGTGG GCGACAAGTC CGGCGTCTTC CCTTCTAACT ATGTGAGGCT TAAAGATTCA 
GAGGGCTCTG GAACTGCTGG GAAAACAGGG AGTTTAGGAA AAAAACCTGA AATCGCACAG 
GTCATTGCCT CCTACACAGC TACAGGTCCT GAACAGCTTA CCCTGGCCCC CGGTCAGCTG 
ATCCTGATCC GGAAAAAGAA CCCAGGTGGA TGGTGGGAAG GAGAGCTACA GGCACGTGGG 
AAAAAGCGCC AGATAGGCTG GTTTCCTGCT AATTACGTCA AACTTTTAAG CCCTGGGACG 
AGCAAAATCA CTCCAACAGA CCCACCAAAG CCAACAGCTT TCCCAACAGT GTGCCAGGTG 
ATCGGGATGT ACGACTACAC CGCGCAGAAC GACGACGAGC TGGCCTTCAG CAAGGGCCAG 
GTCATCAATG TCCTCAACAA GGAGGACCCC GACTGGTGGA AGGGTGAAGT CCACGGGCAG 
GTGGGGCTCT TCCCGTCCAA CTACGTGAAG CTGACCACGG ACACGGACCC CAGCCAGCAA 
TGGTGTTCAG ACTTACACCT CTTGGATATG CTGACCCCAA CTGAAAGAAA GCGCCAGGGG 
TACATCCACG AGCTCATCGT CACAGAGGAG AACTATGTGA ATGACCTGCA GCTGGTCACA 
GAGATCTTTC AGAAACCCCT GATGGAGTCT GAGCTGCTGA CAGAAAAAGA GGGTGCTATG 
ATTTTTGTTA ACTGGAAGGA GCTGATTATG TGTAATATCA AACTACTGAA AGCGCTGAGG 
GTCCGCAAGA AGATGTCTGG GGAGAAGATG CCCGTGAAGA TGATCGGAGA CATCCTGACG 
GCCCAGCTGC CACACATGCA GCCCTACATC CGCTTCTGCA GCTGCCAGCT CAACGGGGCC 
GCCTTGATCC AGCAGAAGAC TGACGAGGCC CCAGACTTCA AGGAGTTTGT TAAAAGGCTG 
GCCATGGACC CACGCTGTAA GGGAATGCCC CTGTCTAGTT TTATACTGAA GCCTATGCAA 
CGAGTAACGA GATACCCACT GATCATTAAA AATATCCTGG AAAACACTCC CGAAAACCAC 
CCAGACCACA GCCACCTGAA GCACGCCCTG GAGAAGGCAG AGGAGCTGTG CTCCCAGGTG 
AACGAAGGGG TGCGGGAGAA GGAGAACTCG GACCGACTGG AGTGGATCCA GGCCCACGTA 
CTGTGTGAAG GCCTGTCAGA GCAACTTGTG TTCAATTCCG TGACCAACTG CCTGGGGCCG 
CGCAAGTTCC TGCACAGTGG GAAGCTCTAC AAGGCCAAGA GCAACAAGGA GCTGTATGGC 
TTCCTCTTCA ATGACTTCCT GCTGCTGACC CAAATCATCA AGCCCCTGGG CTCCTCAGGC 
ACCGACAAAG TCTTCAGCCC CAAATCAAAC CTGCAGTATA AAATGTACAA AACACCTATC 
TTCCTAAATG AGGTTCTAGT GAAGTTGCCC ACTGACCCTT CTGGGGATGA ACCCATCTTC 
CACATTTCCC ACATCGACCG AGTCTACACC CTCCGAGCAG AGAGCATAAA CGAAAGGACT 
GCTTGGGTGC AGAAAATCAA AGCAGCTTCT GAACTCTACA TAGAGACAGA GAAGAAGAAG 
CGGGAGAAGG CGTATCTGGT CCGCTCCCAA AGAGCCACAG GCATCGGAAG GTTGATGGTG 
AACGTGGTTG AAGGCATTGA GCTGAAACCC TGTCGCTCCC ATGGGAAGAG TAACCCATAC 
TGCGAGGTGA CCATGGGTTC CCAGTGTCAC ATCACCAAGA CGATCCAGGA CACTCTGAAT 
CCCAAGTGGA ATTCCAACTG CCAGTTCTTC ATCAGAGACT TGGAGCAAGA GGTGCTGTGC 
ATCACCGTGT TTGAGAGGGA CCAGTTCTCG CCAGATGATT TTTTGGGTCG GACGGAGATC 
CGCGTGGCCG ACATCAAGAA GGACCAGGGC TCCAAGGGTC CAGTTACGAA GTGTCTCCTG 
CTGCACGAAG TTCCCACGGG AGAGATCGTG GTCCGCTTGG ACCTGCAGTT GTTCGATGAG 
CCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITSN1 ( XM_006048226.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006048288.1 Bubalus bubalis intersectin 1 (SH3 domain protein) (ITSN1), transcript variant X2, mRNA.

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CloneID	OBu06695
Clone ID Related Accession (Same CDS sequence)	XM_025281327.1 , XM_025281333.1 , XM_006048226.1 , XM_025281317.1 , XM_025281323.1
Accession Version	XM_006048226.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5166bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	intersectin-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005784098.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCAGT TTCCCACACC TTTTGGTGGC AGCCTGGATA TTTGGGCCAT AACTGTAGAG 
GAAAGAGCAA AGCATGATCA ACAGTTCCAT AGTTTAAAGC CAATATCTGG ATTTATTACT 
GGTGATCAAG CTAGAAACTT TTTTTTTCAA TCTGGGTTAC CTCAGCCTGT TTTAGCACAG 
ATATGGGCGC TCGCTGATAT GAATAATGAT GGAAGGATGG ATCAAGTGGA GTTTTCCATT 
GCTATGAAAC TCATCAAGCT GAAGCTCCAA GGATATCAGC TCCCCTCTGC ACTTCCCCCT 
GTCATGAAAC AGCAACCAGT TGCTATTTCT AGTGCACCAG CGTTTGGTAT GGGAGGTATT 
GCCAGCATGC CGCCGCTGAC CGCTGTTGCT CCTGTGCCAA TGGGCTCCAT TCCCGTTGTT 
GGCATGTCTC CCCCCCTAGT ATCATCTGTT CCTGCAGCAG GGGTGCCCCC CCTGGCCAAC 
GGGGCGCCCC CGGTCATACA GCCTCTGCCG GCATTTGCTC ATCCTGCTGC CACATTGCCA 
AAGAGTTCTT CCTTTAGTAG ATCTGGCCCA GGGTCGCAGT TAAACACAAA ATTACAGAAG 
GCACAATCAT TTGATGTGGC CAGCGTCCCT CCTACAGCTG AGTGGGCTGT TCCTCAGTCA 
TCCAGGCTGA AATACAGACA GCTCTTCAAT AGTCATGACA AAACCATGAG TGGACACCTA 
ACAGGTCCCC AAGCAAGAAC TATTCTTATG CAATCAAGTT TACCACAGGC TCAGCTGGCT 
TCAATATGGA ATCTTTCTGA CATTGATCAA GATGGAAAAC TCACAGCAGA AGAATTTATC 
CTGGCTATGC ACCTCATTGA TGTAGCTATG TCTGGTCAAC CATTGCCACC GGTCCTGCCC 
CCAGAATACA TTCCACCTTC CTTTAGAAGA GTTCGATCTG GTAGTGGTAT ATCTGTCATA 
ACCTCAACTT CGGTGGATCA GAGGTTGCCA GAGGAACCAG TTTTGGAAGA TGAACAGCAA 
CAACTAGAAA AGAAACTACC TGTAACCTTT GAAGATAAGA AGCGGGAGAA CTTTGAACGG 
GGCAACCTGG AGCTGGAGAA GCGGAGACAA GCGCTCTTGG AGCAGCAGCG TAAAGAGCAG 
GAGCGCCTGG CCCAGCTGGA GCGGGCAGAA CAGGAGAGGA AGGAGCGGGA GCGCCAGGAG 
CAGGAGCGCA AAAGACAACT GGAGCTGGAA AAGCAGCTAG AGAAGCAGCG GGAGCTGGAG 
CGGCAGAGGG AGGAGGAGCG AAGGAAGGAA ATCGAGAGGC GGGAGGCTGC GAAACGGGAG 
CTTGAAAGGC AAAGACAGCT TGAATGGGAA CGGAATCGAA GACAAGAACT ATTAAATCAA 
AGAAACAAAG AACAAGAAGA CATAGTTGTA CTGAAAGCAA AGAAAAAGAC TTTGGAATTT 
GAATTAGAAG CTCTAAATGA TAAAAAACAT CAATTAGAAG GAAAACTTCA GGATATCAGA 
TGTCGACTGA CAACCCAAAG GCAAGAAATT GAGAGCACAA ACAAATCTAG AGAGTTGAGA 
ATTGCAGAAA TCACCCACCT ACAACAGCAA TTACAGGAGT CCCAGCAAAT GCTTGGAAGA 
CTTATTCCAG AAAAACAGAT ACTCAATGAC CAATTGAAAC AAGTTCAGCA AAACAGTTTG 
CACAGGGATT CACTTCTCAC ACTCAAAAGA GCCTTAGAAG CGAAAGAACT GGCTCGGCAG 
CAGCTACGAG ACCAACTGGA TGAAGTGGAG AAAGAAACTC GGTCAAAATT ACAGGAGATT 
GATATTTTCA ACAATCAGCT GAAGGAACTT AGAGAAATAC ATAATAAGCA GCAACTCCAG 
AAACAGAAGT CCATAGAGGC TGAACGACTG AAACAGAAGG AACAAGAACG AAAGATCCTG 
GAATTAGAAA AACAAAAAGA AGAAGCCCAA AGACGAGCTC AGGAAAGGGA CCAGCAGTGG 
CTGGAGCGCG TGCATCAGGA GGATGAGCAG CAGCGGCCAC GAAAACCCCA CGAGGAGGAG 
AAGCTCAAAA GGGAAGAGAG CGTCAAAAAG AAGGACAGTG AGGAGAAGGG CAAACCGGAA 
ATGCAAGACA AGCTGAGTCG GCTTTTCCAC CAGCATCAAG AACCAGCTAA GCCCGCTGTC 
CAGGCTCCCT GGTCCACTGC AGAAAAAGGC CCACTTACGA TTTCTGCACA GGAGAATGTA 
AAAGTGGTGT ATTACCGAGC ACTTTATCCC TTTGAGTCCA GGAGTCACGA TGAAATCACG 
ATCCAGCCGG GAGATATAGT CATGGTTAAA GGGGAATGGG TGGACGAAAG CCAAACTGGA 
GAACCGGGAT GGCTTGGAGG AGAATTAAAA GGAAAGACGG GATGGTTCCC TGCAAACTAC 
GCAGAGAAAA TCCCAGAAAA CGAGGTTCCC GCCCCAGTCA AACCAGTGAC TGAGGCGGCC 
TCCACCCCTA CACCCAAAGT GGCTGTGCGT GAGACCCCCG CCCCTTCATC TGCAACCCCT 
GCAGAGCCCT CCGAGACCCC CAACAACTGG GCCGACTTCA GCTCCACGTG GCCCACCAGC 
ACGAATGAGA AACCAGAAAC CGACAGCTGG GACGCATGGG CAACCCAGCC CTCCCTCACC 
GTGCCAAGCG CCGGGCAGCT GAGGCAGAGG TCGGCCTTCA CTCCAGCCAC GGCCACGGGC 
TCATCTCCGT CTCCTGTTCT AGGCCAGGGT GAAAAGGTGG AGGGGCTACA GGCGCAAGCC 
CTGTATCCGT GGAGAGCCAA GAAAGACAAC CATTTAAATT TTAACAAAAA CGATGTCATC 
ACCGTACTGG AGCAGCAAGA CATGTGGTGG TTTGGAGAAG TTCAAGGCCA GAAGGGTTGG 
TTCCCCAAGT CCTACGTGAA ACTCATTTCA GGGCCCATAA GGAAATCGAC AAGCATGGAT 
TCTGGTTCCT CAGAAAGTCC TGCAAGTCTG AAGAGGGTAG CTTCCCCAGC AGCCAAGCCA 
GCTGTGTCGG GAGAAGAATT TATTGCCATG TACACTTACG AGAGTTCTGA GCAAGGCGAT 
TTAACCTTTC AGCAAGGGGA TGTGATTTTG GTTACCAAGA AAGATGGTGA CTGGTGGACA 
GGAACAGTGG GCGACAAGTC CGGCGTCTTC CCTTCTAACT ATGTGAGGCT TAAAGATTCA 
GAGGGCTCTG GAACTGCTGG GAAAACAGGG AGTTTAGGAA AAAAACCTGA AATCGCACAG 
GTCATTGCCT CCTACACAGC TACAGGTCCT GAACAGCTTA CCCTGGCCCC CGGTCAGCTG 
ATCCTGATCC GGAAAAAGAA CCCAGGTGGA TGGTGGGAAG GAGAGCTACA GGCACGTGGG 
AAAAAGCGCC AGATAGGCTG GTTTCCTGCT AATTACGTCA AACTTTTAAG CCCTGGGACG 
AGCAAAATCA CTCCAACAGA CCCACCAAAG CCAACAGCTT TCCCAACAGT GTGCCAGGTG 
ATCGGGATGT ACGACTACAC CGCGCAGAAC GACGACGAGC TGGCCTTCAG CAAGGGCCAG 
GTCATCAATG TCCTCAACAA GGAGGACCCC GACTGGTGGA AGGGTGAAGT CCACGGGCAG 
GTGGGGCTCT TCCCGTCCAA CTACGTGAAG CTGACCACGG ACACGGACCC CAGCCAGCAA 
TGGTGTTCAG ACTTACACCT CTTGGATATG CTGACCCCAA CTGAAAGAAA GCGCCAGGGG 
TACATCCACG AGCTCATCGT CACAGAGGAG AACTATGTGA ATGACCTGCA GCTGGTCACA 
GAGATCTTTC AGAAACCCCT GATGGAGTCT GAGCTGCTGA CAGAAAAAGA GGGTGCTATG 
ATTTTTGTTA ACTGGAAGGA GCTGATTATG TGTAATATCA AACTACTGAA AGCGCTGAGG 
GTCCGCAAGA AGATGTCTGG GGAGAAGATG CCCGTGAAGA TGATCGGAGA CATCCTGACG 
GCCCAGCTGC CACACATGCA GCCCTACATC CGCTTCTGCA GCTGCCAGCT CAACGGGGCC 
GCCTTGATCC AGCAGAAGAC TGACGAGGCC CCAGACTTCA AGGAGTTTGT TAAAAGGCTG 
GCCATGGACC CACGCTGTAA GGGAATGCCC CTGTCTAGTT TTATACTGAA GCCTATGCAA 
CGAGTAACGA GATACCCACT GATCATTAAA AATATCCTGG AAAACACTCC CGAAAACCAC 
CCAGACCACA GCCACCTGAA GCACGCCCTG GAGAAGGCAG AGGAGCTGTG CTCCCAGGTG 
AACGAAGGGG TGCGGGAGAA GGAGAACTCG GACCGACTGG AGTGGATCCA GGCCCACGTA 
CTGTGTGAAG GCCTGTCAGA GCAACTTGTG TTCAATTCCG TGACCAACTG CCTGGGGCCG 
CGCAAGTTCC TGCACAGTGG GAAGCTCTAC AAGGCCAAGA GCAACAAGGA GCTGTATGGC 
TTCCTCTTCA ATGACTTCCT GCTGCTGACC CAAATCATCA AGCCCCTGGG CTCCTCAGGC 
ACCGACAAAG TCTTCAGCCC CAAATCAAAC CTGCAGTATA AAATGTACAA AACACCTATC 
TTCCTAAATG AGGTTCTAGT GAAGTTGCCC ACTGACCCTT CTGGGGATGA ACCCATCTTC 
CACATTTCCC ACATCGACCG AGTCTACACC CTCCGAGCAG AGAGCATAAA CGAAAGGACT 
GCTTGGGTGC AGAAAATCAA AGCAGCTTCT GAACTCTACA TAGAGACAGA GAAGAAGAAG 
CGGGAGAAGG CGTATCTGGT CCGCTCCCAA AGAGCCACAG GCATCGGAAG GTTGATGGTG 
AACGTGGTTG AAGGCATTGA GCTGAAACCC TGTCGCTCCC ATGGGAAGAG TAACCCATAC 
TGCGAGGTGA CCATGGGTTC CCAGTGTCAC ATCACCAAGA CGATCCAGGA CACTCTGAAT 
CCCAAGTGGA ATTCCAACTG CCAGTTCTTC ATCAGAGACT TGGAGCAAGA GGTGCTGTGC 
ATCACCGTGT TTGAGAGGGA CCAGTTCTCG CCAGATGATT TTTTGGGTCG GACGGAGATC 
CGCGTGGCCG ACATCAAGAA GGACCAGGGC TCCAAGGGTC CAGTTACGAA GTGTCTCCTG 
CTGCACGAAG TTCCCACGGG AGAGATCGTG GTCCGCTTGG ACCTGCAGTT GTTCGATGAG 
CCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006048288.1
CDS	58..5223
Translation	MAQFPTPFGGSLDIWAITVEERAKHDQQFHSLKPISGFITGDQARNFFFQSGLPQPVLAQIWALADMNNDGRMDQVEFSIAMKLIKLKLQGYQLPSALPPVMKQQPVAISSAPAFGMGGIASMPPLTAVAPVPMGSIPVVGMSPPLVSSVPAAGVPPLANGAPPVIQPLPAFAHPAATLPKSSSFSRSGPGSQLNTKLQKAQSFDVASVPPTAEWAVPQSSRLKYRQLFNSHDKTMSGHLTGPQARTILMQSSLPQAQLASIWNLSDIDQDGKLTAEEFILAMHLIDVAMSGQPLPPVLPPEYIPPSFRRVRSGSGISVITSTSVDQRLPEEPVLEDEQQQLEKKLPVTFEDKKRENFERGNLELEKRRQALLEQQRKEQERLAQLERAEQERKERERQEQERKRQLELEKQLEKQRELERQREEERRKEIERREAAKRELERQRQLEWERNRRQELLNQRNKEQEDIVVLKAKKKTLEFELEALNDKKHQLEGKLQDIRCRLTTQRQEIESTNKSRELRIAEITHLQQQLQESQQMLGRLIPEKQILNDQLKQVQQNSLHRDSLLTLKRALEAKELARQQLRDQLDEVEKETRSKLQEIDIFNNQLKELREIHNKQQLQKQKSIEAERLKQKEQERKILELEKQKEEAQRRAQERDQQWLERVHQEDEQQRPRKPHEEEKLKREESVKKKDSEEKGKPEMQDKLSRLFHQHQEPAKPAVQAPWSTAEKGPLTISAQENVKVVYYRALYPFESRSHDEITIQPGDIVMVKGEWVDESQTGEPGWLGGELKGKTGWFPANYAEKIPENEVPAPVKPVTEAASTPTPKVAVRETPAPSSATPAEPSETPNNWADFSSTWPTSTNEKPETDSWDAWATQPSLTVPSAGQLRQRSAFTPATATGSSPSPVLGQGEKVEGLQAQALYPWRAKKDNHLNFNKNDVITVLEQQDMWWFGEVQGQKGWFPKSYVKLISGPIRKSTSMDSGSSESPASLKRVASPAAKPAVSGEEFIAMYTYESSEQGDLTFQQGDVILVTKKDGDWWTGTVGDKSGVFPSNYVRLKDSEGSGTAGKTGSLGKKPEIAQVIASYTATGPEQLTLAPGQLILIRKKNPGGWWEGELQARGKKRQIGWFPANYVKLLSPGTSKITPTDPPKPTAFPTVCQVIGMYDYTAQNDDELAFSKGQVINVLNKEDPDWWKGEVHGQVGLFPSNYVKLTTDTDPSQQWCSDLHLLDMLTPTERKRQGYIHELIVTEENYVNDLQLVTEIFQKPLMESELLTEKEGAMIFVNWKELIMCNIKLLKALRVRKKMSGEKMPVKMIGDILTAQLPHMQPYIRFCSCQLNGAALIQQKTDEAPDFKEFVKRLAMDPRCKGMPLSSFILKPMQRVTRYPLIIKNILENTPENHPDHSHLKHALEKAEELCSQVNEGVREKENSDRLEWIQAHVLCEGLSEQLVFNSVTNCLGPRKFLHSGKLYKAKSNKELYGFLFNDFLLLTQIIKPLGSSGTDKVFSPKSNLQYKMYKTPIFLNEVLVKLPTDPSGDEPIFHISHIDRVYTLRAESINERTAWVQKIKAASELYIETEKKKREKAYLVRSQRATGIGRLMVNVVEGIELKPCRSHGKSNPYCEVTMGSQCHITKTIQDTLNPKWNSNCQFFIRDLEQEVLCITVFERDQFSPDDFLGRTEIRVADIKKDQGSKGPVTKCLLLHEVPTGEIVVRLDLQLFDEP

Target ORF information:

RefSeq Version	XM_006048226.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis intersectin 1 (SH3 domain protein) (ITSN1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006048226.1

ORF Insert Sequence:

ATGGCTCAGT TTCCCACACC TTTTGGTGGC AGCCTGGATA TTTGGGCCAT AACTGTAGAG 
GAAAGAGCAA AGCATGATCA ACAGTTCCAT AGTTTAAAGC CAATATCTGG ATTTATTACT 
GGTGATCAAG CTAGAAACTT TTTTTTTCAA TCTGGGTTAC CTCAGCCTGT TTTAGCACAG 
ATATGGGCGC TCGCTGATAT GAATAATGAT GGAAGGATGG ATCAAGTGGA GTTTTCCATT 
GCTATGAAAC TCATCAAGCT GAAGCTCCAA GGATATCAGC TCCCCTCTGC ACTTCCCCCT 
GTCATGAAAC AGCAACCAGT TGCTATTTCT AGTGCACCAG CGTTTGGTAT GGGAGGTATT 
GCCAGCATGC CGCCGCTGAC CGCTGTTGCT CCTGTGCCAA TGGGCTCCAT TCCCGTTGTT 
GGCATGTCTC CCCCCCTAGT ATCATCTGTT CCTGCAGCAG GGGTGCCCCC CCTGGCCAAC 
GGGGCGCCCC CGGTCATACA GCCTCTGCCG GCATTTGCTC ATCCTGCTGC CACATTGCCA 
AAGAGTTCTT CCTTTAGTAG ATCTGGCCCA GGGTCGCAGT TAAACACAAA ATTACAGAAG 
GCACAATCAT TTGATGTGGC CAGCGTCCCT CCTACAGCTG AGTGGGCTGT TCCTCAGTCA 
TCCAGGCTGA AATACAGACA GCTCTTCAAT AGTCATGACA AAACCATGAG TGGACACCTA 
ACAGGTCCCC AAGCAAGAAC TATTCTTATG CAATCAAGTT TACCACAGGC TCAGCTGGCT 
TCAATATGGA ATCTTTCTGA CATTGATCAA GATGGAAAAC TCACAGCAGA AGAATTTATC 
CTGGCTATGC ACCTCATTGA TGTAGCTATG TCTGGTCAAC CATTGCCACC GGTCCTGCCC 
CCAGAATACA TTCCACCTTC CTTTAGAAGA GTTCGATCTG GTAGTGGTAT ATCTGTCATA 
ACCTCAACTT CGGTGGATCA GAGGTTGCCA GAGGAACCAG TTTTGGAAGA TGAACAGCAA 
CAACTAGAAA AGAAACTACC TGTAACCTTT GAAGATAAGA AGCGGGAGAA CTTTGAACGG 
GGCAACCTGG AGCTGGAGAA GCGGAGACAA GCGCTCTTGG AGCAGCAGCG TAAAGAGCAG 
GAGCGCCTGG CCCAGCTGGA GCGGGCAGAA CAGGAGAGGA AGGAGCGGGA GCGCCAGGAG 
CAGGAGCGCA AAAGACAACT GGAGCTGGAA AAGCAGCTAG AGAAGCAGCG GGAGCTGGAG 
CGGCAGAGGG AGGAGGAGCG AAGGAAGGAA ATCGAGAGGC GGGAGGCTGC GAAACGGGAG 
CTTGAAAGGC AAAGACAGCT TGAATGGGAA CGGAATCGAA GACAAGAACT ATTAAATCAA 
AGAAACAAAG AACAAGAAGA CATAGTTGTA CTGAAAGCAA AGAAAAAGAC TTTGGAATTT 
GAATTAGAAG CTCTAAATGA TAAAAAACAT CAATTAGAAG GAAAACTTCA GGATATCAGA 
TGTCGACTGA CAACCCAAAG GCAAGAAATT GAGAGCACAA ACAAATCTAG AGAGTTGAGA 
ATTGCAGAAA TCACCCACCT ACAACAGCAA TTACAGGAGT CCCAGCAAAT GCTTGGAAGA 
CTTATTCCAG AAAAACAGAT ACTCAATGAC CAATTGAAAC AAGTTCAGCA AAACAGTTTG 
CACAGGGATT CACTTCTCAC ACTCAAAAGA GCCTTAGAAG CGAAAGAACT GGCTCGGCAG 
CAGCTACGAG ACCAACTGGA TGAAGTGGAG AAAGAAACTC GGTCAAAATT ACAGGAGATT 
GATATTTTCA ACAATCAGCT GAAGGAACTT AGAGAAATAC ATAATAAGCA GCAACTCCAG 
AAACAGAAGT CCATAGAGGC TGAACGACTG AAACAGAAGG AACAAGAACG AAAGATCCTG 
GAATTAGAAA AACAAAAAGA AGAAGCCCAA AGACGAGCTC AGGAAAGGGA CCAGCAGTGG 
CTGGAGCGCG TGCATCAGGA GGATGAGCAG CAGCGGCCAC GAAAACCCCA CGAGGAGGAG 
AAGCTCAAAA GGGAAGAGAG CGTCAAAAAG AAGGACAGTG AGGAGAAGGG CAAACCGGAA 
ATGCAAGACA AGCTGAGTCG GCTTTTCCAC CAGCATCAAG AACCAGCTAA GCCCGCTGTC 
CAGGCTCCCT GGTCCACTGC AGAAAAAGGC CCACTTACGA TTTCTGCACA GGAGAATGTA 
AAAGTGGTGT ATTACCGAGC ACTTTATCCC TTTGAGTCCA GGAGTCACGA TGAAATCACG 
ATCCAGCCGG GAGATATAGT CATGGTTAAA GGGGAATGGG TGGACGAAAG CCAAACTGGA 
GAACCGGGAT GGCTTGGAGG AGAATTAAAA GGAAAGACGG GATGGTTCCC TGCAAACTAC 
GCAGAGAAAA TCCCAGAAAA CGAGGTTCCC GCCCCAGTCA AACCAGTGAC TGAGGCGGCC 
TCCACCCCTA CACCCAAAGT GGCTGTGCGT GAGACCCCCG CCCCTTCATC TGCAACCCCT 
GCAGAGCCCT CCGAGACCCC CAACAACTGG GCCGACTTCA GCTCCACGTG GCCCACCAGC 
ACGAATGAGA AACCAGAAAC CGACAGCTGG GACGCATGGG CAACCCAGCC CTCCCTCACC 
GTGCCAAGCG CCGGGCAGCT GAGGCAGAGG TCGGCCTTCA CTCCAGCCAC GGCCACGGGC 
TCATCTCCGT CTCCTGTTCT AGGCCAGGGT GAAAAGGTGG AGGGGCTACA GGCGCAAGCC 
CTGTATCCGT GGAGAGCCAA GAAAGACAAC CATTTAAATT TTAACAAAAA CGATGTCATC 
ACCGTACTGG AGCAGCAAGA CATGTGGTGG TTTGGAGAAG TTCAAGGCCA GAAGGGTTGG 
TTCCCCAAGT CCTACGTGAA ACTCATTTCA GGGCCCATAA GGAAATCGAC AAGCATGGAT 
TCTGGTTCCT CAGAAAGTCC TGCAAGTCTG AAGAGGGTAG CTTCCCCAGC AGCCAAGCCA 
GCTGTGTCGG GAGAAGAATT TATTGCCATG TACACTTACG AGAGTTCTGA GCAAGGCGAT 
TTAACCTTTC AGCAAGGGGA TGTGATTTTG GTTACCAAGA AAGATGGTGA CTGGTGGACA 
GGAACAGTGG GCGACAAGTC CGGCGTCTTC CCTTCTAACT ATGTGAGGCT TAAAGATTCA 
GAGGGCTCTG GAACTGCTGG GAAAACAGGG AGTTTAGGAA AAAAACCTGA AATCGCACAG 
GTCATTGCCT CCTACACAGC TACAGGTCCT GAACAGCTTA CCCTGGCCCC CGGTCAGCTG 
ATCCTGATCC GGAAAAAGAA CCCAGGTGGA TGGTGGGAAG GAGAGCTACA GGCACGTGGG 
AAAAAGCGCC AGATAGGCTG GTTTCCTGCT AATTACGTCA AACTTTTAAG CCCTGGGACG 
AGCAAAATCA CTCCAACAGA CCCACCAAAG CCAACAGCTT TCCCAACAGT GTGCCAGGTG 
ATCGGGATGT ACGACTACAC CGCGCAGAAC GACGACGAGC TGGCCTTCAG CAAGGGCCAG 
GTCATCAATG TCCTCAACAA GGAGGACCCC GACTGGTGGA AGGGTGAAGT CCACGGGCAG 
GTGGGGCTCT TCCCGTCCAA CTACGTGAAG CTGACCACGG ACACGGACCC CAGCCAGCAA 
TGGTGTTCAG ACTTACACCT CTTGGATATG CTGACCCCAA CTGAAAGAAA GCGCCAGGGG 
TACATCCACG AGCTCATCGT CACAGAGGAG AACTATGTGA ATGACCTGCA GCTGGTCACA 
GAGATCTTTC AGAAACCCCT GATGGAGTCT GAGCTGCTGA CAGAAAAAGA GGGTGCTATG 
ATTTTTGTTA ACTGGAAGGA GCTGATTATG TGTAATATCA AACTACTGAA AGCGCTGAGG 
GTCCGCAAGA AGATGTCTGG GGAGAAGATG CCCGTGAAGA TGATCGGAGA CATCCTGACG 
GCCCAGCTGC CACACATGCA GCCCTACATC CGCTTCTGCA GCTGCCAGCT CAACGGGGCC 
GCCTTGATCC AGCAGAAGAC TGACGAGGCC CCAGACTTCA AGGAGTTTGT TAAAAGGCTG 
GCCATGGACC CACGCTGTAA GGGAATGCCC CTGTCTAGTT TTATACTGAA GCCTATGCAA 
CGAGTAACGA GATACCCACT GATCATTAAA AATATCCTGG AAAACACTCC CGAAAACCAC 
CCAGACCACA GCCACCTGAA GCACGCCCTG GAGAAGGCAG AGGAGCTGTG CTCCCAGGTG 
AACGAAGGGG TGCGGGAGAA GGAGAACTCG GACCGACTGG AGTGGATCCA GGCCCACGTA 
CTGTGTGAAG GCCTGTCAGA GCAACTTGTG TTCAATTCCG TGACCAACTG CCTGGGGCCG 
CGCAAGTTCC TGCACAGTGG GAAGCTCTAC AAGGCCAAGA GCAACAAGGA GCTGTATGGC 
TTCCTCTTCA ATGACTTCCT GCTGCTGACC CAAATCATCA AGCCCCTGGG CTCCTCAGGC 
ACCGACAAAG TCTTCAGCCC CAAATCAAAC CTGCAGTATA AAATGTACAA AACACCTATC 
TTCCTAAATG AGGTTCTAGT GAAGTTGCCC ACTGACCCTT CTGGGGATGA ACCCATCTTC 
CACATTTCCC ACATCGACCG AGTCTACACC CTCCGAGCAG AGAGCATAAA CGAAAGGACT 
GCTTGGGTGC AGAAAATCAA AGCAGCTTCT GAACTCTACA TAGAGACAGA GAAGAAGAAG 
CGGGAGAAGG CGTATCTGGT CCGCTCCCAA AGAGCCACAG GCATCGGAAG GTTGATGGTG 
AACGTGGTTG AAGGCATTGA GCTGAAACCC TGTCGCTCCC ATGGGAAGAG TAACCCATAC 
TGCGAGGTGA CCATGGGTTC CCAGTGTCAC ATCACCAAGA CGATCCAGGA CACTCTGAAT 
CCCAAGTGGA ATTCCAACTG CCAGTTCTTC ATCAGAGACT TGGAGCAAGA GGTGCTGTGC 
ATCACCGTGT TTGAGAGGGA CCAGTTCTCG CCAGATGATT TTTTGGGTCG GACGGAGATC 
CGCGTGGCCG ACATCAAGAA GGACCAGGGC TCCAAGGGTC CAGTTACGAA GTGTCTCCTG 
CTGCACGAAG TTCCCACGGG AGAGATCGTG GTCCGCTTGG ACCTGCAGTT GTTCGATGAG 
CCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITSN1 ( XM_025281317.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025137102.1 Bubalus bubalis intersectin 1 (ITSN1), transcript variant X3, mRNA.

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CloneID	OBu06695
Clone ID Related Accession (Same CDS sequence)	XM_025281327.1 , XM_025281333.1 , XM_006048226.1 , XM_025281317.1 , XM_025281323.1
Accession Version	XM_025281317.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5166bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	intersectin-1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037545.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCAGT TTCCCACACC TTTTGGTGGC AGCCTGGATA TTTGGGCCAT AACTGTAGAG 
GAAAGAGCAA AGCATGATCA ACAGTTCCAT AGTTTAAAGC CAATATCTGG ATTTATTACT 
GGTGATCAAG CTAGAAACTT TTTTTTTCAA TCTGGGTTAC CTCAGCCTGT TTTAGCACAG 
ATATGGGCGC TCGCTGATAT GAATAATGAT GGAAGGATGG ATCAAGTGGA GTTTTCCATT 
GCTATGAAAC TCATCAAGCT GAAGCTCCAA GGATATCAGC TCCCCTCTGC ACTTCCCCCT 
GTCATGAAAC AGCAACCAGT TGCTATTTCT AGTGCACCAG CGTTTGGTAT GGGAGGTATT 
GCCAGCATGC CGCCGCTGAC CGCTGTTGCT CCTGTGCCAA TGGGCTCCAT TCCCGTTGTT 
GGCATGTCTC CCCCCCTAGT ATCATCTGTT CCTGCAGCAG GGGTGCCCCC CCTGGCCAAC 
GGGGCGCCCC CGGTCATACA GCCTCTGCCG GCATTTGCTC ATCCTGCTGC CACATTGCCA 
AAGAGTTCTT CCTTTAGTAG ATCTGGCCCA GGGTCGCAGT TAAACACAAA ATTACAGAAG 
GCACAATCAT TTGATGTGGC CAGCGTCCCT CCTACAGCTG AGTGGGCTGT TCCTCAGTCA 
TCCAGGCTGA AATACAGACA GCTCTTCAAT AGTCATGACA AAACCATGAG TGGACACCTA 
ACAGGTCCCC AAGCAAGAAC TATTCTTATG CAATCAAGTT TACCACAGGC TCAGCTGGCT 
TCAATATGGA ATCTTTCTGA CATTGATCAA GATGGAAAAC TCACAGCAGA AGAATTTATC 
CTGGCTATGC ACCTCATTGA TGTAGCTATG TCTGGTCAAC CATTGCCACC GGTCCTGCCC 
CCAGAATACA TTCCACCTTC CTTTAGAAGA GTTCGATCTG GTAGTGGTAT ATCTGTCATA 
ACCTCAACTT CGGTGGATCA GAGGTTGCCA GAGGAACCAG TTTTGGAAGA TGAACAGCAA 
CAACTAGAAA AGAAACTACC TGTAACCTTT GAAGATAAGA AGCGGGAGAA CTTTGAACGG 
GGCAACCTGG AGCTGGAGAA GCGGAGACAA GCGCTCTTGG AGCAGCAGCG TAAAGAGCAG 
GAGCGCCTGG CCCAGCTGGA GCGGGCAGAA CAGGAGAGGA AGGAGCGGGA GCGCCAGGAG 
CAGGAGCGCA AAAGACAACT GGAGCTGGAA AAGCAGCTAG AGAAGCAGCG GGAGCTGGAG 
CGGCAGAGGG AGGAGGAGCG AAGGAAGGAA ATCGAGAGGC GGGAGGCTGC GAAACGGGAG 
CTTGAAAGGC AAAGACAGCT TGAATGGGAA CGGAATCGAA GACAAGAACT ATTAAATCAA 
AGAAACAAAG AACAAGAAGA CATAGTTGTA CTGAAAGCAA AGAAAAAGAC TTTGGAATTT 
GAATTAGAAG CTCTAAATGA TAAAAAACAT CAATTAGAAG GAAAACTTCA GGATATCAGA 
TGTCGACTGA CAACCCAAAG GCAAGAAATT GAGAGCACAA ACAAATCTAG AGAGTTGAGA 
ATTGCAGAAA TCACCCACCT ACAACAGCAA TTACAGGAGT CCCAGCAAAT GCTTGGAAGA 
CTTATTCCAG AAAAACAGAT ACTCAATGAC CAATTGAAAC AAGTTCAGCA AAACAGTTTG 
CACAGGGATT CACTTCTCAC ACTCAAAAGA GCCTTAGAAG CGAAAGAACT GGCTCGGCAG 
CAGCTACGAG ACCAACTGGA TGAAGTGGAG AAAGAAACTC GGTCAAAATT ACAGGAGATT 
GATATTTTCA ACAATCAGCT GAAGGAACTT AGAGAAATAC ATAATAAGCA GCAACTCCAG 
AAACAGAAGT CCATAGAGGC TGAACGACTG AAACAGAAGG AACAAGAACG AAAGATCCTG 
GAATTAGAAA AACAAAAAGA AGAAGCCCAA AGACGAGCTC AGGAAAGGGA CCAGCAGTGG 
CTGGAGCGCG TGCATCAGGA GGATGAGCAG CAGCGGCCAC GAAAACCCCA CGAGGAGGAG 
AAGCTCAAAA GGGAAGAGAG CGTCAAAAAG AAGGACAGTG AGGAGAAGGG CAAACCGGAA 
ATGCAAGACA AGCTGAGTCG GCTTTTCCAC CAGCATCAAG AACCAGCTAA GCCCGCTGTC 
CAGGCTCCCT GGTCCACTGC AGAAAAAGGC CCACTTACGA TTTCTGCACA GGAGAATGTA 
AAAGTGGTGT ATTACCGAGC ACTTTATCCC TTTGAGTCCA GGAGTCACGA TGAAATCACG 
ATCCAGCCGG GAGATATAGT CATGGTTAAA GGGGAATGGG TGGACGAAAG CCAAACTGGA 
GAACCGGGAT GGCTTGGAGG AGAATTAAAA GGAAAGACGG GATGGTTCCC TGCAAACTAC 
GCAGAGAAAA TCCCAGAAAA CGAGGTTCCC GCCCCAGTCA AACCAGTGAC TGAGGCGGCC 
TCCACCCCTA CACCCAAAGT GGCTGTGCGT GAGACCCCCG CCCCTTCATC TGCAACCCCT 
GCAGAGCCCT CCGAGACCCC CAACAACTGG GCCGACTTCA GCTCCACGTG GCCCACCAGC 
ACGAATGAGA AACCAGAAAC CGACAGCTGG GACGCATGGG CAACCCAGCC CTCCCTCACC 
GTGCCAAGCG CCGGGCAGCT GAGGCAGAGG TCGGCCTTCA CTCCAGCCAC GGCCACGGGC 
TCATCTCCGT CTCCTGTTCT AGGCCAGGGT GAAAAGGTGG AGGGGCTACA GGCGCAAGCC 
CTGTATCCGT GGAGAGCCAA GAAAGACAAC CATTTAAATT TTAACAAAAA CGATGTCATC 
ACCGTACTGG AGCAGCAAGA CATGTGGTGG TTTGGAGAAG TTCAAGGCCA GAAGGGTTGG 
TTCCCCAAGT CCTACGTGAA ACTCATTTCA GGGCCCATAA GGAAATCGAC AAGCATGGAT 
TCTGGTTCCT CAGAAAGTCC TGCAAGTCTG AAGAGGGTAG CTTCCCCAGC AGCCAAGCCA 
GCTGTGTCGG GAGAAGAATT TATTGCCATG TACACTTACG AGAGTTCTGA GCAAGGCGAT 
TTAACCTTTC AGCAAGGGGA TGTGATTTTG GTTACCAAGA AAGATGGTGA CTGGTGGACA 
GGAACAGTGG GCGACAAGTC CGGCGTCTTC CCTTCTAACT ATGTGAGGCT TAAAGATTCA 
GAGGGCTCTG GAACTGCTGG GAAAACAGGG AGTTTAGGAA AAAAACCTGA AATCGCACAG 
GTCATTGCCT CCTACACAGC TACAGGTCCT GAACAGCTTA CCCTGGCCCC CGGTCAGCTG 
ATCCTGATCC GGAAAAAGAA CCCAGGTGGA TGGTGGGAAG GAGAGCTACA GGCACGTGGG 
AAAAAGCGCC AGATAGGCTG GTTTCCTGCT AATTACGTCA AACTTTTAAG CCCTGGGACG 
AGCAAAATCA CTCCAACAGA CCCACCAAAG CCAACAGCTT TCCCAACAGT GTGCCAGGTG 
ATCGGGATGT ACGACTACAC CGCGCAGAAC GACGACGAGC TGGCCTTCAG CAAGGGCCAG 
GTCATCAATG TCCTCAACAA GGAGGACCCC GACTGGTGGA AGGGTGAAGT CCACGGGCAG 
GTGGGGCTCT TCCCGTCCAA CTACGTGAAG CTGACCACGG ACACGGACCC CAGCCAGCAA 
TGGTGTTCAG ACTTACACCT CTTGGATATG CTGACCCCAA CTGAAAGAAA GCGCCAGGGG 
TACATCCACG AGCTCATCGT CACAGAGGAG AACTATGTGA ATGACCTGCA GCTGGTCACA 
GAGATCTTTC AGAAACCCCT GATGGAGTCT GAGCTGCTGA CAGAAAAAGA GGGTGCTATG 
ATTTTTGTTA ACTGGAAGGA GCTGATTATG TGTAATATCA AACTACTGAA AGCGCTGAGG 
GTCCGCAAGA AGATGTCTGG GGAGAAGATG CCCGTGAAGA TGATCGGAGA CATCCTGACG 
GCCCAGCTGC CACACATGCA GCCCTACATC CGCTTCTGCA GCTGCCAGCT CAACGGGGCC 
GCCTTGATCC AGCAGAAGAC TGACGAGGCC CCAGACTTCA AGGAGTTTGT TAAAAGGCTG 
GCCATGGACC CACGCTGTAA GGGAATGCCC CTGTCTAGTT TTATACTGAA GCCTATGCAA 
CGAGTAACGA GATACCCACT GATCATTAAA AATATCCTGG AAAACACTCC CGAAAACCAC 
CCAGACCACA GCCACCTGAA GCACGCCCTG GAGAAGGCAG AGGAGCTGTG CTCCCAGGTG 
AACGAAGGGG TGCGGGAGAA GGAGAACTCG GACCGACTGG AGTGGATCCA GGCCCACGTA 
CTGTGTGAAG GCCTGTCAGA GCAACTTGTG TTCAATTCCG TGACCAACTG CCTGGGGCCG 
CGCAAGTTCC TGCACAGTGG GAAGCTCTAC AAGGCCAAGA GCAACAAGGA GCTGTATGGC 
TTCCTCTTCA ATGACTTCCT GCTGCTGACC CAAATCATCA AGCCCCTGGG CTCCTCAGGC 
ACCGACAAAG TCTTCAGCCC CAAATCAAAC CTGCAGTATA AAATGTACAA AACACCTATC 
TTCCTAAATG AGGTTCTAGT GAAGTTGCCC ACTGACCCTT CTGGGGATGA ACCCATCTTC 
CACATTTCCC ACATCGACCG AGTCTACACC CTCCGAGCAG AGAGCATAAA CGAAAGGACT 
GCTTGGGTGC AGAAAATCAA AGCAGCTTCT GAACTCTACA TAGAGACAGA GAAGAAGAAG 
CGGGAGAAGG CGTATCTGGT CCGCTCCCAA AGAGCCACAG GCATCGGAAG GTTGATGGTG 
AACGTGGTTG AAGGCATTGA GCTGAAACCC TGTCGCTCCC ATGGGAAGAG TAACCCATAC 
TGCGAGGTGA CCATGGGTTC CCAGTGTCAC ATCACCAAGA CGATCCAGGA CACTCTGAAT 
CCCAAGTGGA ATTCCAACTG CCAGTTCTTC ATCAGAGACT TGGAGCAAGA GGTGCTGTGC 
ATCACCGTGT TTGAGAGGGA CCAGTTCTCG CCAGATGATT TTTTGGGTCG GACGGAGATC 
CGCGTGGCCG ACATCAAGAA GGACCAGGGC TCCAAGGGTC CAGTTACGAA GTGTCTCCTG 
CTGCACGAAG TTCCCACGGG AGAGATCGTG GTCCGCTTGG ACCTGCAGTT GTTCGATGAG 
CCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025137102.1
CDS	302..5467
Translation	MAQFPTPFGGSLDIWAITVEERAKHDQQFHSLKPISGFITGDQARNFFFQSGLPQPVLAQIWALADMNNDGRMDQVEFSIAMKLIKLKLQGYQLPSALPPVMKQQPVAISSAPAFGMGGIASMPPLTAVAPVPMGSIPVVGMSPPLVSSVPAAGVPPLANGAPPVIQPLPAFAHPAATLPKSSSFSRSGPGSQLNTKLQKAQSFDVASVPPTAEWAVPQSSRLKYRQLFNSHDKTMSGHLTGPQARTILMQSSLPQAQLASIWNLSDIDQDGKLTAEEFILAMHLIDVAMSGQPLPPVLPPEYIPPSFRRVRSGSGISVITSTSVDQRLPEEPVLEDEQQQLEKKLPVTFEDKKRENFERGNLELEKRRQALLEQQRKEQERLAQLERAEQERKERERQEQERKRQLELEKQLEKQRELERQREEERRKEIERREAAKRELERQRQLEWERNRRQELLNQRNKEQEDIVVLKAKKKTLEFELEALNDKKHQLEGKLQDIRCRLTTQRQEIESTNKSRELRIAEITHLQQQLQESQQMLGRLIPEKQILNDQLKQVQQNSLHRDSLLTLKRALEAKELARQQLRDQLDEVEKETRSKLQEIDIFNNQLKELREIHNKQQLQKQKSIEAERLKQKEQERKILELEKQKEEAQRRAQERDQQWLERVHQEDEQQRPRKPHEEEKLKREESVKKKDSEEKGKPEMQDKLSRLFHQHQEPAKPAVQAPWSTAEKGPLTISAQENVKVVYYRALYPFESRSHDEITIQPGDIVMVKGEWVDESQTGEPGWLGGELKGKTGWFPANYAEKIPENEVPAPVKPVTEAASTPTPKVAVRETPAPSSATPAEPSETPNNWADFSSTWPTSTNEKPETDSWDAWATQPSLTVPSAGQLRQRSAFTPATATGSSPSPVLGQGEKVEGLQAQALYPWRAKKDNHLNFNKNDVITVLEQQDMWWFGEVQGQKGWFPKSYVKLISGPIRKSTSMDSGSSESPASLKRVASPAAKPAVSGEEFIAMYTYESSEQGDLTFQQGDVILVTKKDGDWWTGTVGDKSGVFPSNYVRLKDSEGSGTAGKTGSLGKKPEIAQVIASYTATGPEQLTLAPGQLILIRKKNPGGWWEGELQARGKKRQIGWFPANYVKLLSPGTSKITPTDPPKPTAFPTVCQVIGMYDYTAQNDDELAFSKGQVINVLNKEDPDWWKGEVHGQVGLFPSNYVKLTTDTDPSQQWCSDLHLLDMLTPTERKRQGYIHELIVTEENYVNDLQLVTEIFQKPLMESELLTEKEGAMIFVNWKELIMCNIKLLKALRVRKKMSGEKMPVKMIGDILTAQLPHMQPYIRFCSCQLNGAALIQQKTDEAPDFKEFVKRLAMDPRCKGMPLSSFILKPMQRVTRYPLIIKNILENTPENHPDHSHLKHALEKAEELCSQVNEGVREKENSDRLEWIQAHVLCEGLSEQLVFNSVTNCLGPRKFLHSGKLYKAKSNKELYGFLFNDFLLLTQIIKPLGSSGTDKVFSPKSNLQYKMYKTPIFLNEVLVKLPTDPSGDEPIFHISHIDRVYTLRAESINERTAWVQKIKAASELYIETEKKKREKAYLVRSQRATGIGRLMVNVVEGIELKPCRSHGKSNPYCEVTMGSQCHITKTIQDTLNPKWNSNCQFFIRDLEQEVLCITVFERDQFSPDDFLGRTEIRVADIKKDQGSKGPVTKCLLLHEVPTGEIVVRLDLQLFDEP

Target ORF information:

RefSeq Version	XM_025281317.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis intersectin 1 (ITSN1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025281317.1

ORF Insert Sequence:

ATGGCTCAGT TTCCCACACC TTTTGGTGGC AGCCTGGATA TTTGGGCCAT AACTGTAGAG 
GAAAGAGCAA AGCATGATCA ACAGTTCCAT AGTTTAAAGC CAATATCTGG ATTTATTACT 
GGTGATCAAG CTAGAAACTT TTTTTTTCAA TCTGGGTTAC CTCAGCCTGT TTTAGCACAG 
ATATGGGCGC TCGCTGATAT GAATAATGAT GGAAGGATGG ATCAAGTGGA GTTTTCCATT 
GCTATGAAAC TCATCAAGCT GAAGCTCCAA GGATATCAGC TCCCCTCTGC ACTTCCCCCT 
GTCATGAAAC AGCAACCAGT TGCTATTTCT AGTGCACCAG CGTTTGGTAT GGGAGGTATT 
GCCAGCATGC CGCCGCTGAC CGCTGTTGCT CCTGTGCCAA TGGGCTCCAT TCCCGTTGTT 
GGCATGTCTC CCCCCCTAGT ATCATCTGTT CCTGCAGCAG GGGTGCCCCC CCTGGCCAAC 
GGGGCGCCCC CGGTCATACA GCCTCTGCCG GCATTTGCTC ATCCTGCTGC CACATTGCCA 
AAGAGTTCTT CCTTTAGTAG ATCTGGCCCA GGGTCGCAGT TAAACACAAA ATTACAGAAG 
GCACAATCAT TTGATGTGGC CAGCGTCCCT CCTACAGCTG AGTGGGCTGT TCCTCAGTCA 
TCCAGGCTGA AATACAGACA GCTCTTCAAT AGTCATGACA AAACCATGAG TGGACACCTA 
ACAGGTCCCC AAGCAAGAAC TATTCTTATG CAATCAAGTT TACCACAGGC TCAGCTGGCT 
TCAATATGGA ATCTTTCTGA CATTGATCAA GATGGAAAAC TCACAGCAGA AGAATTTATC 
CTGGCTATGC ACCTCATTGA TGTAGCTATG TCTGGTCAAC CATTGCCACC GGTCCTGCCC 
CCAGAATACA TTCCACCTTC CTTTAGAAGA GTTCGATCTG GTAGTGGTAT ATCTGTCATA 
ACCTCAACTT CGGTGGATCA GAGGTTGCCA GAGGAACCAG TTTTGGAAGA TGAACAGCAA 
CAACTAGAAA AGAAACTACC TGTAACCTTT GAAGATAAGA AGCGGGAGAA CTTTGAACGG 
GGCAACCTGG AGCTGGAGAA GCGGAGACAA GCGCTCTTGG AGCAGCAGCG TAAAGAGCAG 
GAGCGCCTGG CCCAGCTGGA GCGGGCAGAA CAGGAGAGGA AGGAGCGGGA GCGCCAGGAG 
CAGGAGCGCA AAAGACAACT GGAGCTGGAA AAGCAGCTAG AGAAGCAGCG GGAGCTGGAG 
CGGCAGAGGG AGGAGGAGCG AAGGAAGGAA ATCGAGAGGC GGGAGGCTGC GAAACGGGAG 
CTTGAAAGGC AAAGACAGCT TGAATGGGAA CGGAATCGAA GACAAGAACT ATTAAATCAA 
AGAAACAAAG AACAAGAAGA CATAGTTGTA CTGAAAGCAA AGAAAAAGAC TTTGGAATTT 
GAATTAGAAG CTCTAAATGA TAAAAAACAT CAATTAGAAG GAAAACTTCA GGATATCAGA 
TGTCGACTGA CAACCCAAAG GCAAGAAATT GAGAGCACAA ACAAATCTAG AGAGTTGAGA 
ATTGCAGAAA TCACCCACCT ACAACAGCAA TTACAGGAGT CCCAGCAAAT GCTTGGAAGA 
CTTATTCCAG AAAAACAGAT ACTCAATGAC CAATTGAAAC AAGTTCAGCA AAACAGTTTG 
CACAGGGATT CACTTCTCAC ACTCAAAAGA GCCTTAGAAG CGAAAGAACT GGCTCGGCAG 
CAGCTACGAG ACCAACTGGA TGAAGTGGAG AAAGAAACTC GGTCAAAATT ACAGGAGATT 
GATATTTTCA ACAATCAGCT GAAGGAACTT AGAGAAATAC ATAATAAGCA GCAACTCCAG 
AAACAGAAGT CCATAGAGGC TGAACGACTG AAACAGAAGG AACAAGAACG AAAGATCCTG 
GAATTAGAAA AACAAAAAGA AGAAGCCCAA AGACGAGCTC AGGAAAGGGA CCAGCAGTGG 
CTGGAGCGCG TGCATCAGGA GGATGAGCAG CAGCGGCCAC GAAAACCCCA CGAGGAGGAG 
AAGCTCAAAA GGGAAGAGAG CGTCAAAAAG AAGGACAGTG AGGAGAAGGG CAAACCGGAA 
ATGCAAGACA AGCTGAGTCG GCTTTTCCAC CAGCATCAAG AACCAGCTAA GCCCGCTGTC 
CAGGCTCCCT GGTCCACTGC AGAAAAAGGC CCACTTACGA TTTCTGCACA GGAGAATGTA 
AAAGTGGTGT ATTACCGAGC ACTTTATCCC TTTGAGTCCA GGAGTCACGA TGAAATCACG 
ATCCAGCCGG GAGATATAGT CATGGTTAAA GGGGAATGGG TGGACGAAAG CCAAACTGGA 
GAACCGGGAT GGCTTGGAGG AGAATTAAAA GGAAAGACGG GATGGTTCCC TGCAAACTAC 
GCAGAGAAAA TCCCAGAAAA CGAGGTTCCC GCCCCAGTCA AACCAGTGAC TGAGGCGGCC 
TCCACCCCTA CACCCAAAGT GGCTGTGCGT GAGACCCCCG CCCCTTCATC TGCAACCCCT 
GCAGAGCCCT CCGAGACCCC CAACAACTGG GCCGACTTCA GCTCCACGTG GCCCACCAGC 
ACGAATGAGA AACCAGAAAC CGACAGCTGG GACGCATGGG CAACCCAGCC CTCCCTCACC 
GTGCCAAGCG CCGGGCAGCT GAGGCAGAGG TCGGCCTTCA CTCCAGCCAC GGCCACGGGC 
TCATCTCCGT CTCCTGTTCT AGGCCAGGGT GAAAAGGTGG AGGGGCTACA GGCGCAAGCC 
CTGTATCCGT GGAGAGCCAA GAAAGACAAC CATTTAAATT TTAACAAAAA CGATGTCATC 
ACCGTACTGG AGCAGCAAGA CATGTGGTGG TTTGGAGAAG TTCAAGGCCA GAAGGGTTGG 
TTCCCCAAGT CCTACGTGAA ACTCATTTCA GGGCCCATAA GGAAATCGAC AAGCATGGAT 
TCTGGTTCCT CAGAAAGTCC TGCAAGTCTG AAGAGGGTAG CTTCCCCAGC AGCCAAGCCA 
GCTGTGTCGG GAGAAGAATT TATTGCCATG TACACTTACG AGAGTTCTGA GCAAGGCGAT 
TTAACCTTTC AGCAAGGGGA TGTGATTTTG GTTACCAAGA AAGATGGTGA CTGGTGGACA 
GGAACAGTGG GCGACAAGTC CGGCGTCTTC CCTTCTAACT ATGTGAGGCT TAAAGATTCA 
GAGGGCTCTG GAACTGCTGG GAAAACAGGG AGTTTAGGAA AAAAACCTGA AATCGCACAG 
GTCATTGCCT CCTACACAGC TACAGGTCCT GAACAGCTTA CCCTGGCCCC CGGTCAGCTG 
ATCCTGATCC GGAAAAAGAA CCCAGGTGGA TGGTGGGAAG GAGAGCTACA GGCACGTGGG 
AAAAAGCGCC AGATAGGCTG GTTTCCTGCT AATTACGTCA AACTTTTAAG CCCTGGGACG 
AGCAAAATCA CTCCAACAGA CCCACCAAAG CCAACAGCTT TCCCAACAGT GTGCCAGGTG 
ATCGGGATGT ACGACTACAC CGCGCAGAAC GACGACGAGC TGGCCTTCAG CAAGGGCCAG 
GTCATCAATG TCCTCAACAA GGAGGACCCC GACTGGTGGA AGGGTGAAGT CCACGGGCAG 
GTGGGGCTCT TCCCGTCCAA CTACGTGAAG CTGACCACGG ACACGGACCC CAGCCAGCAA 
TGGTGTTCAG ACTTACACCT CTTGGATATG CTGACCCCAA CTGAAAGAAA GCGCCAGGGG 
TACATCCACG AGCTCATCGT CACAGAGGAG AACTATGTGA ATGACCTGCA GCTGGTCACA 
GAGATCTTTC AGAAACCCCT GATGGAGTCT GAGCTGCTGA CAGAAAAAGA GGGTGCTATG 
ATTTTTGTTA ACTGGAAGGA GCTGATTATG TGTAATATCA AACTACTGAA AGCGCTGAGG 
GTCCGCAAGA AGATGTCTGG GGAGAAGATG CCCGTGAAGA TGATCGGAGA CATCCTGACG 
GCCCAGCTGC CACACATGCA GCCCTACATC CGCTTCTGCA GCTGCCAGCT CAACGGGGCC 
GCCTTGATCC AGCAGAAGAC TGACGAGGCC CCAGACTTCA AGGAGTTTGT TAAAAGGCTG 
GCCATGGACC CACGCTGTAA GGGAATGCCC CTGTCTAGTT TTATACTGAA GCCTATGCAA 
CGAGTAACGA GATACCCACT GATCATTAAA AATATCCTGG AAAACACTCC CGAAAACCAC 
CCAGACCACA GCCACCTGAA GCACGCCCTG GAGAAGGCAG AGGAGCTGTG CTCCCAGGTG 
AACGAAGGGG TGCGGGAGAA GGAGAACTCG GACCGACTGG AGTGGATCCA GGCCCACGTA 
CTGTGTGAAG GCCTGTCAGA GCAACTTGTG TTCAATTCCG TGACCAACTG CCTGGGGCCG 
CGCAAGTTCC TGCACAGTGG GAAGCTCTAC AAGGCCAAGA GCAACAAGGA GCTGTATGGC 
TTCCTCTTCA ATGACTTCCT GCTGCTGACC CAAATCATCA AGCCCCTGGG CTCCTCAGGC 
ACCGACAAAG TCTTCAGCCC CAAATCAAAC CTGCAGTATA AAATGTACAA AACACCTATC 
TTCCTAAATG AGGTTCTAGT GAAGTTGCCC ACTGACCCTT CTGGGGATGA ACCCATCTTC 
CACATTTCCC ACATCGACCG AGTCTACACC CTCCGAGCAG AGAGCATAAA CGAAAGGACT 
GCTTGGGTGC AGAAAATCAA AGCAGCTTCT GAACTCTACA TAGAGACAGA GAAGAAGAAG 
CGGGAGAAGG CGTATCTGGT CCGCTCCCAA AGAGCCACAG GCATCGGAAG GTTGATGGTG 
AACGTGGTTG AAGGCATTGA GCTGAAACCC TGTCGCTCCC ATGGGAAGAG TAACCCATAC 
TGCGAGGTGA CCATGGGTTC CCAGTGTCAC ATCACCAAGA CGATCCAGGA CACTCTGAAT 
CCCAAGTGGA ATTCCAACTG CCAGTTCTTC ATCAGAGACT TGGAGCAAGA GGTGCTGTGC 
ATCACCGTGT TTGAGAGGGA CCAGTTCTCG CCAGATGATT TTTTGGGTCG GACGGAGATC 
CGCGTGGCCG ACATCAAGAA GGACCAGGGC TCCAAGGGTC CAGTTACGAA GTGTCTCCTG 
CTGCACGAAG TTCCCACGGG AGAGATCGTG GTCCGCTTGG ACCTGCAGTT GTTCGATGAG 
CCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITSN1 ( XM_025281323.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025137108.1 Bubalus bubalis intersectin 1 (ITSN1), transcript variant X4, mRNA.

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CloneID	OBu06695
Clone ID Related Accession (Same CDS sequence)	XM_025281327.1 , XM_025281333.1 , XM_006048226.1 , XM_025281317.1 , XM_025281323.1
Accession Version	XM_025281323.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5166bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	intersectin-1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037545.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCAGT TTCCCACACC TTTTGGTGGC AGCCTGGATA TTTGGGCCAT AACTGTAGAG 
GAAAGAGCAA AGCATGATCA ACAGTTCCAT AGTTTAAAGC CAATATCTGG ATTTATTACT 
GGTGATCAAG CTAGAAACTT TTTTTTTCAA TCTGGGTTAC CTCAGCCTGT TTTAGCACAG 
ATATGGGCGC TCGCTGATAT GAATAATGAT GGAAGGATGG ATCAAGTGGA GTTTTCCATT 
GCTATGAAAC TCATCAAGCT GAAGCTCCAA GGATATCAGC TCCCCTCTGC ACTTCCCCCT 
GTCATGAAAC AGCAACCAGT TGCTATTTCT AGTGCACCAG CGTTTGGTAT GGGAGGTATT 
GCCAGCATGC CGCCGCTGAC CGCTGTTGCT CCTGTGCCAA TGGGCTCCAT TCCCGTTGTT 
GGCATGTCTC CCCCCCTAGT ATCATCTGTT CCTGCAGCAG GGGTGCCCCC CCTGGCCAAC 
GGGGCGCCCC CGGTCATACA GCCTCTGCCG GCATTTGCTC ATCCTGCTGC CACATTGCCA 
AAGAGTTCTT CCTTTAGTAG ATCTGGCCCA GGGTCGCAGT TAAACACAAA ATTACAGAAG 
GCACAATCAT TTGATGTGGC CAGCGTCCCT CCTACAGCTG AGTGGGCTGT TCCTCAGTCA 
TCCAGGCTGA AATACAGACA GCTCTTCAAT AGTCATGACA AAACCATGAG TGGACACCTA 
ACAGGTCCCC AAGCAAGAAC TATTCTTATG CAATCAAGTT TACCACAGGC TCAGCTGGCT 
TCAATATGGA ATCTTTCTGA CATTGATCAA GATGGAAAAC TCACAGCAGA AGAATTTATC 
CTGGCTATGC ACCTCATTGA TGTAGCTATG TCTGGTCAAC CATTGCCACC GGTCCTGCCC 
CCAGAATACA TTCCACCTTC CTTTAGAAGA GTTCGATCTG GTAGTGGTAT ATCTGTCATA 
ACCTCAACTT CGGTGGATCA GAGGTTGCCA GAGGAACCAG TTTTGGAAGA TGAACAGCAA 
CAACTAGAAA AGAAACTACC TGTAACCTTT GAAGATAAGA AGCGGGAGAA CTTTGAACGG 
GGCAACCTGG AGCTGGAGAA GCGGAGACAA GCGCTCTTGG AGCAGCAGCG TAAAGAGCAG 
GAGCGCCTGG CCCAGCTGGA GCGGGCAGAA CAGGAGAGGA AGGAGCGGGA GCGCCAGGAG 
CAGGAGCGCA AAAGACAACT GGAGCTGGAA AAGCAGCTAG AGAAGCAGCG GGAGCTGGAG 
CGGCAGAGGG AGGAGGAGCG AAGGAAGGAA ATCGAGAGGC GGGAGGCTGC GAAACGGGAG 
CTTGAAAGGC AAAGACAGCT TGAATGGGAA CGGAATCGAA GACAAGAACT ATTAAATCAA 
AGAAACAAAG AACAAGAAGA CATAGTTGTA CTGAAAGCAA AGAAAAAGAC TTTGGAATTT 
GAATTAGAAG CTCTAAATGA TAAAAAACAT CAATTAGAAG GAAAACTTCA GGATATCAGA 
TGTCGACTGA CAACCCAAAG GCAAGAAATT GAGAGCACAA ACAAATCTAG AGAGTTGAGA 
ATTGCAGAAA TCACCCACCT ACAACAGCAA TTACAGGAGT CCCAGCAAAT GCTTGGAAGA 
CTTATTCCAG AAAAACAGAT ACTCAATGAC CAATTGAAAC AAGTTCAGCA AAACAGTTTG 
CACAGGGATT CACTTCTCAC ACTCAAAAGA GCCTTAGAAG CGAAAGAACT GGCTCGGCAG 
CAGCTACGAG ACCAACTGGA TGAAGTGGAG AAAGAAACTC GGTCAAAATT ACAGGAGATT 
GATATTTTCA ACAATCAGCT GAAGGAACTT AGAGAAATAC ATAATAAGCA GCAACTCCAG 
AAACAGAAGT CCATAGAGGC TGAACGACTG AAACAGAAGG AACAAGAACG AAAGATCCTG 
GAATTAGAAA AACAAAAAGA AGAAGCCCAA AGACGAGCTC AGGAAAGGGA CCAGCAGTGG 
CTGGAGCGCG TGCATCAGGA GGATGAGCAG CAGCGGCCAC GAAAACCCCA CGAGGAGGAG 
AAGCTCAAAA GGGAAGAGAG CGTCAAAAAG AAGGACAGTG AGGAGAAGGG CAAACCGGAA 
ATGCAAGACA AGCTGAGTCG GCTTTTCCAC CAGCATCAAG AACCAGCTAA GCCCGCTGTC 
CAGGCTCCCT GGTCCACTGC AGAAAAAGGC CCACTTACGA TTTCTGCACA GGAGAATGTA 
AAAGTGGTGT ATTACCGAGC ACTTTATCCC TTTGAGTCCA GGAGTCACGA TGAAATCACG 
ATCCAGCCGG GAGATATAGT CATGGTTAAA GGGGAATGGG TGGACGAAAG CCAAACTGGA 
GAACCGGGAT GGCTTGGAGG AGAATTAAAA GGAAAGACGG GATGGTTCCC TGCAAACTAC 
GCAGAGAAAA TCCCAGAAAA CGAGGTTCCC GCCCCAGTCA AACCAGTGAC TGAGGCGGCC 
TCCACCCCTA CACCCAAAGT GGCTGTGCGT GAGACCCCCG CCCCTTCATC TGCAACCCCT 
GCAGAGCCCT CCGAGACCCC CAACAACTGG GCCGACTTCA GCTCCACGTG GCCCACCAGC 
ACGAATGAGA AACCAGAAAC CGACAGCTGG GACGCATGGG CAACCCAGCC CTCCCTCACC 
GTGCCAAGCG CCGGGCAGCT GAGGCAGAGG TCGGCCTTCA CTCCAGCCAC GGCCACGGGC 
TCATCTCCGT CTCCTGTTCT AGGCCAGGGT GAAAAGGTGG AGGGGCTACA GGCGCAAGCC 
CTGTATCCGT GGAGAGCCAA GAAAGACAAC CATTTAAATT TTAACAAAAA CGATGTCATC 
ACCGTACTGG AGCAGCAAGA CATGTGGTGG TTTGGAGAAG TTCAAGGCCA GAAGGGTTGG 
TTCCCCAAGT CCTACGTGAA ACTCATTTCA GGGCCCATAA GGAAATCGAC AAGCATGGAT 
TCTGGTTCCT CAGAAAGTCC TGCAAGTCTG AAGAGGGTAG CTTCCCCAGC AGCCAAGCCA 
GCTGTGTCGG GAGAAGAATT TATTGCCATG TACACTTACG AGAGTTCTGA GCAAGGCGAT 
TTAACCTTTC AGCAAGGGGA TGTGATTTTG GTTACCAAGA AAGATGGTGA CTGGTGGACA 
GGAACAGTGG GCGACAAGTC CGGCGTCTTC CCTTCTAACT ATGTGAGGCT TAAAGATTCA 
GAGGGCTCTG GAACTGCTGG GAAAACAGGG AGTTTAGGAA AAAAACCTGA AATCGCACAG 
GTCATTGCCT CCTACACAGC TACAGGTCCT GAACAGCTTA CCCTGGCCCC CGGTCAGCTG 
ATCCTGATCC GGAAAAAGAA CCCAGGTGGA TGGTGGGAAG GAGAGCTACA GGCACGTGGG 
AAAAAGCGCC AGATAGGCTG GTTTCCTGCT AATTACGTCA AACTTTTAAG CCCTGGGACG 
AGCAAAATCA CTCCAACAGA CCCACCAAAG CCAACAGCTT TCCCAACAGT GTGCCAGGTG 
ATCGGGATGT ACGACTACAC CGCGCAGAAC GACGACGAGC TGGCCTTCAG CAAGGGCCAG 
GTCATCAATG TCCTCAACAA GGAGGACCCC GACTGGTGGA AGGGTGAAGT CCACGGGCAG 
GTGGGGCTCT TCCCGTCCAA CTACGTGAAG CTGACCACGG ACACGGACCC CAGCCAGCAA 
TGGTGTTCAG ACTTACACCT CTTGGATATG CTGACCCCAA CTGAAAGAAA GCGCCAGGGG 
TACATCCACG AGCTCATCGT CACAGAGGAG AACTATGTGA ATGACCTGCA GCTGGTCACA 
GAGATCTTTC AGAAACCCCT GATGGAGTCT GAGCTGCTGA CAGAAAAAGA GGGTGCTATG 
ATTTTTGTTA ACTGGAAGGA GCTGATTATG TGTAATATCA AACTACTGAA AGCGCTGAGG 
GTCCGCAAGA AGATGTCTGG GGAGAAGATG CCCGTGAAGA TGATCGGAGA CATCCTGACG 
GCCCAGCTGC CACACATGCA GCCCTACATC CGCTTCTGCA GCTGCCAGCT CAACGGGGCC 
GCCTTGATCC AGCAGAAGAC TGACGAGGCC CCAGACTTCA AGGAGTTTGT TAAAAGGCTG 
GCCATGGACC CACGCTGTAA GGGAATGCCC CTGTCTAGTT TTATACTGAA GCCTATGCAA 
CGAGTAACGA GATACCCACT GATCATTAAA AATATCCTGG AAAACACTCC CGAAAACCAC 
CCAGACCACA GCCACCTGAA GCACGCCCTG GAGAAGGCAG AGGAGCTGTG CTCCCAGGTG 
AACGAAGGGG TGCGGGAGAA GGAGAACTCG GACCGACTGG AGTGGATCCA GGCCCACGTA 
CTGTGTGAAG GCCTGTCAGA GCAACTTGTG TTCAATTCCG TGACCAACTG CCTGGGGCCG 
CGCAAGTTCC TGCACAGTGG GAAGCTCTAC AAGGCCAAGA GCAACAAGGA GCTGTATGGC 
TTCCTCTTCA ATGACTTCCT GCTGCTGACC CAAATCATCA AGCCCCTGGG CTCCTCAGGC 
ACCGACAAAG TCTTCAGCCC CAAATCAAAC CTGCAGTATA AAATGTACAA AACACCTATC 
TTCCTAAATG AGGTTCTAGT GAAGTTGCCC ACTGACCCTT CTGGGGATGA ACCCATCTTC 
CACATTTCCC ACATCGACCG AGTCTACACC CTCCGAGCAG AGAGCATAAA CGAAAGGACT 
GCTTGGGTGC AGAAAATCAA AGCAGCTTCT GAACTCTACA TAGAGACAGA GAAGAAGAAG 
CGGGAGAAGG CGTATCTGGT CCGCTCCCAA AGAGCCACAG GCATCGGAAG GTTGATGGTG 
AACGTGGTTG AAGGCATTGA GCTGAAACCC TGTCGCTCCC ATGGGAAGAG TAACCCATAC 
TGCGAGGTGA CCATGGGTTC CCAGTGTCAC ATCACCAAGA CGATCCAGGA CACTCTGAAT 
CCCAAGTGGA ATTCCAACTG CCAGTTCTTC ATCAGAGACT TGGAGCAAGA GGTGCTGTGC 
ATCACCGTGT TTGAGAGGGA CCAGTTCTCG CCAGATGATT TTTTGGGTCG GACGGAGATC 
CGCGTGGCCG ACATCAAGAA GGACCAGGGC TCCAAGGGTC CAGTTACGAA GTGTCTCCTG 
CTGCACGAAG TTCCCACGGG AGAGATCGTG GTCCGCTTGG ACCTGCAGTT GTTCGATGAG 
CCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025137108.1
CDS	466..5631
Translation	MAQFPTPFGGSLDIWAITVEERAKHDQQFHSLKPISGFITGDQARNFFFQSGLPQPVLAQIWALADMNNDGRMDQVEFSIAMKLIKLKLQGYQLPSALPPVMKQQPVAISSAPAFGMGGIASMPPLTAVAPVPMGSIPVVGMSPPLVSSVPAAGVPPLANGAPPVIQPLPAFAHPAATLPKSSSFSRSGPGSQLNTKLQKAQSFDVASVPPTAEWAVPQSSRLKYRQLFNSHDKTMSGHLTGPQARTILMQSSLPQAQLASIWNLSDIDQDGKLTAEEFILAMHLIDVAMSGQPLPPVLPPEYIPPSFRRVRSGSGISVITSTSVDQRLPEEPVLEDEQQQLEKKLPVTFEDKKRENFERGNLELEKRRQALLEQQRKEQERLAQLERAEQERKERERQEQERKRQLELEKQLEKQRELERQREEERRKEIERREAAKRELERQRQLEWERNRRQELLNQRNKEQEDIVVLKAKKKTLEFELEALNDKKHQLEGKLQDIRCRLTTQRQEIESTNKSRELRIAEITHLQQQLQESQQMLGRLIPEKQILNDQLKQVQQNSLHRDSLLTLKRALEAKELARQQLRDQLDEVEKETRSKLQEIDIFNNQLKELREIHNKQQLQKQKSIEAERLKQKEQERKILELEKQKEEAQRRAQERDQQWLERVHQEDEQQRPRKPHEEEKLKREESVKKKDSEEKGKPEMQDKLSRLFHQHQEPAKPAVQAPWSTAEKGPLTISAQENVKVVYYRALYPFESRSHDEITIQPGDIVMVKGEWVDESQTGEPGWLGGELKGKTGWFPANYAEKIPENEVPAPVKPVTEAASTPTPKVAVRETPAPSSATPAEPSETPNNWADFSSTWPTSTNEKPETDSWDAWATQPSLTVPSAGQLRQRSAFTPATATGSSPSPVLGQGEKVEGLQAQALYPWRAKKDNHLNFNKNDVITVLEQQDMWWFGEVQGQKGWFPKSYVKLISGPIRKSTSMDSGSSESPASLKRVASPAAKPAVSGEEFIAMYTYESSEQGDLTFQQGDVILVTKKDGDWWTGTVGDKSGVFPSNYVRLKDSEGSGTAGKTGSLGKKPEIAQVIASYTATGPEQLTLAPGQLILIRKKNPGGWWEGELQARGKKRQIGWFPANYVKLLSPGTSKITPTDPPKPTAFPTVCQVIGMYDYTAQNDDELAFSKGQVINVLNKEDPDWWKGEVHGQVGLFPSNYVKLTTDTDPSQQWCSDLHLLDMLTPTERKRQGYIHELIVTEENYVNDLQLVTEIFQKPLMESELLTEKEGAMIFVNWKELIMCNIKLLKALRVRKKMSGEKMPVKMIGDILTAQLPHMQPYIRFCSCQLNGAALIQQKTDEAPDFKEFVKRLAMDPRCKGMPLSSFILKPMQRVTRYPLIIKNILENTPENHPDHSHLKHALEKAEELCSQVNEGVREKENSDRLEWIQAHVLCEGLSEQLVFNSVTNCLGPRKFLHSGKLYKAKSNKELYGFLFNDFLLLTQIIKPLGSSGTDKVFSPKSNLQYKMYKTPIFLNEVLVKLPTDPSGDEPIFHISHIDRVYTLRAESINERTAWVQKIKAASELYIETEKKKREKAYLVRSQRATGIGRLMVNVVEGIELKPCRSHGKSNPYCEVTMGSQCHITKTIQDTLNPKWNSNCQFFIRDLEQEVLCITVFERDQFSPDDFLGRTEIRVADIKKDQGSKGPVTKCLLLHEVPTGEIVVRLDLQLFDEP

Target ORF information:

RefSeq Version	XM_025281323.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis intersectin 1 (ITSN1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025281323.1

ORF Insert Sequence:

ATGGCTCAGT TTCCCACACC TTTTGGTGGC AGCCTGGATA TTTGGGCCAT AACTGTAGAG 
GAAAGAGCAA AGCATGATCA ACAGTTCCAT AGTTTAAAGC CAATATCTGG ATTTATTACT 
GGTGATCAAG CTAGAAACTT TTTTTTTCAA TCTGGGTTAC CTCAGCCTGT TTTAGCACAG 
ATATGGGCGC TCGCTGATAT GAATAATGAT GGAAGGATGG ATCAAGTGGA GTTTTCCATT 
GCTATGAAAC TCATCAAGCT GAAGCTCCAA GGATATCAGC TCCCCTCTGC ACTTCCCCCT 
GTCATGAAAC AGCAACCAGT TGCTATTTCT AGTGCACCAG CGTTTGGTAT GGGAGGTATT 
GCCAGCATGC CGCCGCTGAC CGCTGTTGCT CCTGTGCCAA TGGGCTCCAT TCCCGTTGTT 
GGCATGTCTC CCCCCCTAGT ATCATCTGTT CCTGCAGCAG GGGTGCCCCC CCTGGCCAAC 
GGGGCGCCCC CGGTCATACA GCCTCTGCCG GCATTTGCTC ATCCTGCTGC CACATTGCCA 
AAGAGTTCTT CCTTTAGTAG ATCTGGCCCA GGGTCGCAGT TAAACACAAA ATTACAGAAG 
GCACAATCAT TTGATGTGGC CAGCGTCCCT CCTACAGCTG AGTGGGCTGT TCCTCAGTCA 
TCCAGGCTGA AATACAGACA GCTCTTCAAT AGTCATGACA AAACCATGAG TGGACACCTA 
ACAGGTCCCC AAGCAAGAAC TATTCTTATG CAATCAAGTT TACCACAGGC TCAGCTGGCT 
TCAATATGGA ATCTTTCTGA CATTGATCAA GATGGAAAAC TCACAGCAGA AGAATTTATC 
CTGGCTATGC ACCTCATTGA TGTAGCTATG TCTGGTCAAC CATTGCCACC GGTCCTGCCC 
CCAGAATACA TTCCACCTTC CTTTAGAAGA GTTCGATCTG GTAGTGGTAT ATCTGTCATA 
ACCTCAACTT CGGTGGATCA GAGGTTGCCA GAGGAACCAG TTTTGGAAGA TGAACAGCAA 
CAACTAGAAA AGAAACTACC TGTAACCTTT GAAGATAAGA AGCGGGAGAA CTTTGAACGG 
GGCAACCTGG AGCTGGAGAA GCGGAGACAA GCGCTCTTGG AGCAGCAGCG TAAAGAGCAG 
GAGCGCCTGG CCCAGCTGGA GCGGGCAGAA CAGGAGAGGA AGGAGCGGGA GCGCCAGGAG 
CAGGAGCGCA AAAGACAACT GGAGCTGGAA AAGCAGCTAG AGAAGCAGCG GGAGCTGGAG 
CGGCAGAGGG AGGAGGAGCG AAGGAAGGAA ATCGAGAGGC GGGAGGCTGC GAAACGGGAG 
CTTGAAAGGC AAAGACAGCT TGAATGGGAA CGGAATCGAA GACAAGAACT ATTAAATCAA 
AGAAACAAAG AACAAGAAGA CATAGTTGTA CTGAAAGCAA AGAAAAAGAC TTTGGAATTT 
GAATTAGAAG CTCTAAATGA TAAAAAACAT CAATTAGAAG GAAAACTTCA GGATATCAGA 
TGTCGACTGA CAACCCAAAG GCAAGAAATT GAGAGCACAA ACAAATCTAG AGAGTTGAGA 
ATTGCAGAAA TCACCCACCT ACAACAGCAA TTACAGGAGT CCCAGCAAAT GCTTGGAAGA 
CTTATTCCAG AAAAACAGAT ACTCAATGAC CAATTGAAAC AAGTTCAGCA AAACAGTTTG 
CACAGGGATT CACTTCTCAC ACTCAAAAGA GCCTTAGAAG CGAAAGAACT GGCTCGGCAG 
CAGCTACGAG ACCAACTGGA TGAAGTGGAG AAAGAAACTC GGTCAAAATT ACAGGAGATT 
GATATTTTCA ACAATCAGCT GAAGGAACTT AGAGAAATAC ATAATAAGCA GCAACTCCAG 
AAACAGAAGT CCATAGAGGC TGAACGACTG AAACAGAAGG AACAAGAACG AAAGATCCTG 
GAATTAGAAA AACAAAAAGA AGAAGCCCAA AGACGAGCTC AGGAAAGGGA CCAGCAGTGG 
CTGGAGCGCG TGCATCAGGA GGATGAGCAG CAGCGGCCAC GAAAACCCCA CGAGGAGGAG 
AAGCTCAAAA GGGAAGAGAG CGTCAAAAAG AAGGACAGTG AGGAGAAGGG CAAACCGGAA 
ATGCAAGACA AGCTGAGTCG GCTTTTCCAC CAGCATCAAG AACCAGCTAA GCCCGCTGTC 
CAGGCTCCCT GGTCCACTGC AGAAAAAGGC CCACTTACGA TTTCTGCACA GGAGAATGTA 
AAAGTGGTGT ATTACCGAGC ACTTTATCCC TTTGAGTCCA GGAGTCACGA TGAAATCACG 
ATCCAGCCGG GAGATATAGT CATGGTTAAA GGGGAATGGG TGGACGAAAG CCAAACTGGA 
GAACCGGGAT GGCTTGGAGG AGAATTAAAA GGAAAGACGG GATGGTTCCC TGCAAACTAC 
GCAGAGAAAA TCCCAGAAAA CGAGGTTCCC GCCCCAGTCA AACCAGTGAC TGAGGCGGCC 
TCCACCCCTA CACCCAAAGT GGCTGTGCGT GAGACCCCCG CCCCTTCATC TGCAACCCCT 
GCAGAGCCCT CCGAGACCCC CAACAACTGG GCCGACTTCA GCTCCACGTG GCCCACCAGC 
ACGAATGAGA AACCAGAAAC CGACAGCTGG GACGCATGGG CAACCCAGCC CTCCCTCACC 
GTGCCAAGCG CCGGGCAGCT GAGGCAGAGG TCGGCCTTCA CTCCAGCCAC GGCCACGGGC 
TCATCTCCGT CTCCTGTTCT AGGCCAGGGT GAAAAGGTGG AGGGGCTACA GGCGCAAGCC 
CTGTATCCGT GGAGAGCCAA GAAAGACAAC CATTTAAATT TTAACAAAAA CGATGTCATC 
ACCGTACTGG AGCAGCAAGA CATGTGGTGG TTTGGAGAAG TTCAAGGCCA GAAGGGTTGG 
TTCCCCAAGT CCTACGTGAA ACTCATTTCA GGGCCCATAA GGAAATCGAC AAGCATGGAT 
TCTGGTTCCT CAGAAAGTCC TGCAAGTCTG AAGAGGGTAG CTTCCCCAGC AGCCAAGCCA 
GCTGTGTCGG GAGAAGAATT TATTGCCATG TACACTTACG AGAGTTCTGA GCAAGGCGAT 
TTAACCTTTC AGCAAGGGGA TGTGATTTTG GTTACCAAGA AAGATGGTGA CTGGTGGACA 
GGAACAGTGG GCGACAAGTC CGGCGTCTTC CCTTCTAACT ATGTGAGGCT TAAAGATTCA 
GAGGGCTCTG GAACTGCTGG GAAAACAGGG AGTTTAGGAA AAAAACCTGA AATCGCACAG 
GTCATTGCCT CCTACACAGC TACAGGTCCT GAACAGCTTA CCCTGGCCCC CGGTCAGCTG 
ATCCTGATCC GGAAAAAGAA CCCAGGTGGA TGGTGGGAAG GAGAGCTACA GGCACGTGGG 
AAAAAGCGCC AGATAGGCTG GTTTCCTGCT AATTACGTCA AACTTTTAAG CCCTGGGACG 
AGCAAAATCA CTCCAACAGA CCCACCAAAG CCAACAGCTT TCCCAACAGT GTGCCAGGTG 
ATCGGGATGT ACGACTACAC CGCGCAGAAC GACGACGAGC TGGCCTTCAG CAAGGGCCAG 
GTCATCAATG TCCTCAACAA GGAGGACCCC GACTGGTGGA AGGGTGAAGT CCACGGGCAG 
GTGGGGCTCT TCCCGTCCAA CTACGTGAAG CTGACCACGG ACACGGACCC CAGCCAGCAA 
TGGTGTTCAG ACTTACACCT CTTGGATATG CTGACCCCAA CTGAAAGAAA GCGCCAGGGG 
TACATCCACG AGCTCATCGT CACAGAGGAG AACTATGTGA ATGACCTGCA GCTGGTCACA 
GAGATCTTTC AGAAACCCCT GATGGAGTCT GAGCTGCTGA CAGAAAAAGA GGGTGCTATG 
ATTTTTGTTA ACTGGAAGGA GCTGATTATG TGTAATATCA AACTACTGAA AGCGCTGAGG 
GTCCGCAAGA AGATGTCTGG GGAGAAGATG CCCGTGAAGA TGATCGGAGA CATCCTGACG 
GCCCAGCTGC CACACATGCA GCCCTACATC CGCTTCTGCA GCTGCCAGCT CAACGGGGCC 
GCCTTGATCC AGCAGAAGAC TGACGAGGCC CCAGACTTCA AGGAGTTTGT TAAAAGGCTG 
GCCATGGACC CACGCTGTAA GGGAATGCCC CTGTCTAGTT TTATACTGAA GCCTATGCAA 
CGAGTAACGA GATACCCACT GATCATTAAA AATATCCTGG AAAACACTCC CGAAAACCAC 
CCAGACCACA GCCACCTGAA GCACGCCCTG GAGAAGGCAG AGGAGCTGTG CTCCCAGGTG 
AACGAAGGGG TGCGGGAGAA GGAGAACTCG GACCGACTGG AGTGGATCCA GGCCCACGTA 
CTGTGTGAAG GCCTGTCAGA GCAACTTGTG TTCAATTCCG TGACCAACTG CCTGGGGCCG 
CGCAAGTTCC TGCACAGTGG GAAGCTCTAC AAGGCCAAGA GCAACAAGGA GCTGTATGGC 
TTCCTCTTCA ATGACTTCCT GCTGCTGACC CAAATCATCA AGCCCCTGGG CTCCTCAGGC 
ACCGACAAAG TCTTCAGCCC CAAATCAAAC CTGCAGTATA AAATGTACAA AACACCTATC 
TTCCTAAATG AGGTTCTAGT GAAGTTGCCC ACTGACCCTT CTGGGGATGA ACCCATCTTC 
CACATTTCCC ACATCGACCG AGTCTACACC CTCCGAGCAG AGAGCATAAA CGAAAGGACT 
GCTTGGGTGC AGAAAATCAA AGCAGCTTCT GAACTCTACA TAGAGACAGA GAAGAAGAAG 
CGGGAGAAGG CGTATCTGGT CCGCTCCCAA AGAGCCACAG GCATCGGAAG GTTGATGGTG 
AACGTGGTTG AAGGCATTGA GCTGAAACCC TGTCGCTCCC ATGGGAAGAG TAACCCATAC 
TGCGAGGTGA CCATGGGTTC CCAGTGTCAC ATCACCAAGA CGATCCAGGA CACTCTGAAT 
CCCAAGTGGA ATTCCAACTG CCAGTTCTTC ATCAGAGACT TGGAGCAAGA GGTGCTGTGC 
ATCACCGTGT TTGAGAGGGA CCAGTTCTCG CCAGATGATT TTTTGGGTCG GACGGAGATC 
CGCGTGGCCG ACATCAAGAA GGACCAGGGC TCCAAGGGTC CAGTTACGAA GTGTCTCCTG 
CTGCACGAAG TTCCCACGGG AGAGATCGTG GTCCGCTTGG ACCTGCAGTT GTTCGATGAG 
CCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITSN1 ( XM_025281309.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025137094.1 Bubalus bubalis intersectin 1 (ITSN1), transcript variant X1, mRNA.

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CloneID	OBu42475
Clone ID Related Accession (Same CDS sequence)	XM_025281309.1
Accession Version	XM_025281309.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5328bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	intersectin-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037545.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTTTGA TCCCTCGGTC GGGCAGATGC CCTGGGGAAG GGAATAGCTA CCCACTCCAG 
TATTCTTGCC TGGAGAATCC CATGGACAGA AGAGCCTGGT GGAGCCCTAA AGTGAAGACA 
GTCTCAGAAA TGAGGGGACA TCTGGTCAGT CCAAGAGTTG AAACTGAAGA ACTTAACCCT 
GTTTTCTCTG GCAGCCTGGA TATTTGGGCC ATAACTGTAG AGGAAAGAGC AAAGCATGAT 
CAACAGTTCC ATAGTTTAAA GCCAATATCT GGATTTATTA CTGGTGATCA AGCTAGAAAC 
TTTTTTTTTC AATCTGGGTT ACCTCAGCCT GTTTTAGCAC AGATATGGGC GCTCGCTGAT 
ATGAATAATG ATGGAAGGAT GGATCAAGTG GAGTTTTCCA TTGCTATGAA ACTCATCAAG 
CTGAAGCTCC AAGGATATCA GCTCCCCTCT GCACTTCCCC CTGTCATGAA ACAGCAACCA 
GTTGCTATTT CTAGTGCACC AGCGTTTGGT ATGGGAGGTA TTGCCAGCAT GCCGCCGCTG 
ACCGCTGTTG CTCCTGTGCC AATGGGCTCC ATTCCCGTTG TTGGCATGTC TCCCCCCCTA 
GTATCATCTG TTCCTGCAGC AGGGGTGCCC CCCCTGGCCA ACGGGGCGCC CCCGGTCATA 
CAGCCTCTGC CGGCATTTGC TCATCCTGCT GCCACATTGC CAAAGAGTTC TTCCTTTAGT 
AGATCTGGCC CAGGGTCGCA GTTAAACACA AAATTACAGA AGGCACAATC ATTTGATGTG 
GCCAGCGTCC CTCCTACAGC TGAGTGGGCT GTTCCTCAGT CATCCAGGCT GAAATACAGA 
CAGCTCTTCA ATAGTCATGA CAAAACCATG AGTGGACACC TAACAGGTCC CCAAGCAAGA 
ACTATTCTTA TGCAATCAAG TTTACCACAG GCTCAGCTGG CTTCAATATG GAATCTTTCT 
GACATTGATC AAGATGGAAA ACTCACAGCA GAAGAATTTA TCCTGGCTAT GCACCTCATT 
GATGTAGCTA TGTCTGGTCA ACCATTGCCA CCGGTCCTGC CCCCAGAATA CATTCCACCT 
TCCTTTAGAA GAGTTCGATC TGGTAGTGGT ATATCTGTCA TAACCTCAAC TTCGGTGGAT 
CAGAGGTTGC CAGAGGAACC AGTTTTGGAA GATGAACAGC AACAACTAGA AAAGAAACTA 
CCTGTAACCT TTGAAGATAA GAAGCGGGAG AACTTTGAAC GGGGCAACCT GGAGCTGGAG 
AAGCGGAGAC AAGCGCTCTT GGAGCAGCAG CGTAAAGAGC AGGAGCGCCT GGCCCAGCTG 
GAGCGGGCAG AACAGGAGAG GAAGGAGCGG GAGCGCCAGG AGCAGGAGCG CAAAAGACAA 
CTGGAGCTGG AAAAGCAGCT AGAGAAGCAG CGGGAGCTGG AGCGGCAGAG GGAGGAGGAG 
CGAAGGAAGG AAATCGAGAG GCGGGAGGCT GCGAAACGGG AGCTTGAAAG GCAAAGACAG 
CTTGAATGGG AACGGAATCG AAGACAAGAA CTATTAAATC AAAGAAACAA AGAACAAGAA 
GACATAGTTG TACTGAAAGC AAAGAAAAAG ACTTTGGAAT TTGAATTAGA AGCTCTAAAT 
GATAAAAAAC ATCAATTAGA AGGAAAACTT CAGGATATCA GATGTCGACT GACAACCCAA 
AGGCAAGAAA TTGAGAGCAC AAACAAATCT AGAGAGTTGA GAATTGCAGA AATCACCCAC 
CTACAACAGC AATTACAGGA GTCCCAGCAA ATGCTTGGAA GACTTATTCC AGAAAAACAG 
ATACTCAATG ACCAATTGAA ACAAGTTCAG CAAAACAGTT TGCACAGGGA TTCACTTCTC 
ACACTCAAAA GAGCCTTAGA AGCGAAAGAA CTGGCTCGGC AGCAGCTACG AGACCAACTG 
GATGAAGTGG AGAAAGAAAC TCGGTCAAAA TTACAGGAGA TTGATATTTT CAACAATCAG 
CTGAAGGAAC TTAGAGAAAT ACATAATAAG CAGCAACTCC AGAAACAGAA GTCCATAGAG 
GCTGAACGAC TGAAACAGAA GGAACAAGAA CGAAAGATCC TGGAATTAGA AAAACAAAAA 
GAAGAAGCCC AAAGACGAGC TCAGGAAAGG GACCAGCAGT GGCTGGAGCG CGTGCATCAG 
GAGGATGAGC AGCAGCGGCC ACGAAAACCC CACGAGGAGG AGAAGCTCAA AAGGGAAGAG 
AGCGTCAAAA AGAAGGACAG TGAGGAGAAG GGCAAACCGG AAATGCAAGA CAAGCTGAGT 
CGGCTTTTCC ACCAGCATCA AGAACCAGCT AAGCCCGCTG TCCAGGCTCC CTGGTCCACT 
GCAGAAAAAG GCCCACTTAC GATTTCTGCA CAGGAGAATG TAAAAGTGGT GTATTACCGA 
GCACTTTATC CCTTTGAGTC CAGGAGTCAC GATGAAATCA CGATCCAGCC GGGAGATATA 
GTCATGGTTA AAGGGGAATG GGTGGACGAA AGCCAAACTG GAGAACCGGG ATGGCTTGGA 
GGAGAATTAA AAGGAAAGAC GGGATGGTTC CCTGCAAACT ACGCAGAGAA AATCCCAGAA 
AACGAGGTTC CCGCCCCAGT CAAACCAGTG ACTGAGGCGG CCTCCACCCC TACACCCAAA 
GTGGCTGTGC GTGAGACCCC CGCCCCTTCA TCTGCAACCC CTGCAGAGCC CTCCGAGACC 
CCCAACAACT GGGCCGACTT CAGCTCCACG TGGCCCACCA GCACGAATGA GAAACCAGAA 
ACCGACAGCT GGGACGCATG GGCAACCCAG CCCTCCCTCA CCGTGCCAAG CGCCGGGCAG 
CTGAGGCAGA GGTCGGCCTT CACTCCAGCC ACGGCCACGG GCTCATCTCC GTCTCCTGTT 
CTAGGCCAGG GTGAAAAGGT GGAGGGGCTA CAGGCGCAAG CCCTGTATCC GTGGAGAGCC 
AAGAAAGACA ACCATTTAAA TTTTAACAAA AACGATGTCA TCACCGTACT GGAGCAGCAA 
GACATGTGGT GGTTTGGAGA AGTTCAAGGC CAGAAGGGTT GGTTCCCCAA GTCCTACGTG 
AAACTCATTT CAGGGCCCAT AAGGAAATCG ACAAGCATGG ATTCTGGTTC CTCAGAAAGT 
CCTGCAAGTC TGAAGAGGGT AGCTTCCCCA GCAGCCAAGC CAGCTGTGTC GGGAGAAGAA 
TTTATTGCCA TGTACACTTA CGAGAGTTCT GAGCAAGGCG ATTTAACCTT TCAGCAAGGG 
GATGTGATTT TGGTTACCAA GAAAGATGGT GACTGGTGGA CAGGAACAGT GGGCGACAAG 
TCCGGCGTCT TCCCTTCTAA CTATGTGAGG CTTAAAGATT CAGAGGGCTC TGGAACTGCT 
GGGAAAACAG GGAGTTTAGG AAAAAAACCT GAAATCGCAC AGGTCATTGC CTCCTACACA 
GCTACAGGTC CTGAACAGCT TACCCTGGCC CCCGGTCAGC TGATCCTGAT CCGGAAAAAG 
AACCCAGGTG GATGGTGGGA AGGAGAGCTA CAGGCACGTG GGAAAAAGCG CCAGATAGGC 
TGGTTTCCTG CTAATTACGT CAAACTTTTA AGCCCTGGGA CGAGCAAAAT CACTCCAACA 
GACCCACCAA AGCCAACAGC TTTCCCAACA GTGTGCCAGG TGATCGGGAT GTACGACTAC 
ACCGCGCAGA ACGACGACGA GCTGGCCTTC AGCAAGGGCC AGGTCATCAA TGTCCTCAAC 
AAGGAGGACC CCGACTGGTG GAAGGGTGAA GTCCACGGGC AGGTGGGGCT CTTCCCGTCC 
AACTACGTGA AGCTGACCAC GGACACGGAC CCCAGCCAGC AATGGTGTTC AGACTTACAC 
CTCTTGGATA TGCTGACCCC AACTGAAAGA AAGCGCCAGG GGTACATCCA CGAGCTCATC 
GTCACAGAGG AGAACTATGT GAATGACCTG CAGCTGGTCA CAGAGATCTT TCAGAAACCC 
CTGATGGAGT CTGAGCTGCT GACAGAAAAA GAGGGTGCTA TGATTTTTGT TAACTGGAAG 
GAGCTGATTA TGTGTAATAT CAAACTACTG AAAGCGCTGA GGGTCCGCAA GAAGATGTCT 
GGGGAGAAGA TGCCCGTGAA GATGATCGGA GACATCCTGA CGGCCCAGCT GCCACACATG 
CAGCCCTACA TCCGCTTCTG CAGCTGCCAG CTCAACGGGG CCGCCTTGAT CCAGCAGAAG 
ACTGACGAGG CCCCAGACTT CAAGGAGTTT GTTAAAAGGC TGGCCATGGA CCCACGCTGT 
AAGGGAATGC CCCTGTCTAG TTTTATACTG AAGCCTATGC AACGAGTAAC GAGATACCCA 
CTGATCATTA AAAATATCCT GGAAAACACT CCCGAAAACC ACCCAGACCA CAGCCACCTG 
AAGCACGCCC TGGAGAAGGC AGAGGAGCTG TGCTCCCAGG TGAACGAAGG GGTGCGGGAG 
AAGGAGAACT CGGACCGACT GGAGTGGATC CAGGCCCACG TACTGTGTGA AGGCCTGTCA 
GAGCAACTTG TGTTCAATTC CGTGACCAAC TGCCTGGGGC CGCGCAAGTT CCTGCACAGT 
GGGAAGCTCT ACAAGGCCAA GAGCAACAAG GAGCTGTATG GCTTCCTCTT CAATGACTTC 
CTGCTGCTGA CCCAAATCAT CAAGCCCCTG GGCTCCTCAG GCACCGACAA AGTCTTCAGC 
CCCAAATCAA ACCTGCAGTA TAAAATGTAC AAAACACCTA TCTTCCTAAA TGAGGTTCTA 
GTGAAGTTGC CCACTGACCC TTCTGGGGAT GAACCCATCT TCCACATTTC CCACATCGAC 
CGAGTCTACA CCCTCCGAGC AGAGAGCATA AACGAAAGGA CTGCTTGGGT GCAGAAAATC 
AAAGCAGCTT CTGAACTCTA CATAGAGACA GAGAAGAAGA AGCGGGAGAA GGCGTATCTG 
GTCCGCTCCC AAAGAGCCAC AGGCATCGGA AGGTTGATGG TGAACGTGGT TGAAGGCATT 
GAGCTGAAAC CCTGTCGCTC CCATGGGAAG AGTAACCCAT ACTGCGAGGT GACCATGGGT 
TCCCAGTGTC ACATCACCAA GACGATCCAG GACACTCTGA ATCCCAAGTG GAATTCCAAC 
TGCCAGTTCT TCATCAGAGA CTTGGAGCAA GAGGTGCTGT GCATCACCGT GTTTGAGAGG 
GACCAGTTCT CGCCAGATGA TTTTTTGGGT CGGACGGAGA TCCGCGTGGC CGACATCAAG 
AAGGACCAGG GCTCCAAGGG TCCAGTTACG AAGTGTCTCC TGCTGCACGA AGTTCCCACG 
GGAGAGATCG TGGTCCGCTT GGACCTGCAG TTGTTCGATG AGCCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025137094.1
CDS	394..5721
Translation	MGLIPRSGRCPGEGNSYPLQYSCLENPMDRRAWWSPKVKTVSEMRGHLVSPRVETEELNPVFSGSLDIWAITVEERAKHDQQFHSLKPISGFITGDQARNFFFQSGLPQPVLAQIWALADMNNDGRMDQVEFSIAMKLIKLKLQGYQLPSALPPVMKQQPVAISSAPAFGMGGIASMPPLTAVAPVPMGSIPVVGMSPPLVSSVPAAGVPPLANGAPPVIQPLPAFAHPAATLPKSSSFSRSGPGSQLNTKLQKAQSFDVASVPPTAEWAVPQSSRLKYRQLFNSHDKTMSGHLTGPQARTILMQSSLPQAQLASIWNLSDIDQDGKLTAEEFILAMHLIDVAMSGQPLPPVLPPEYIPPSFRRVRSGSGISVITSTSVDQRLPEEPVLEDEQQQLEKKLPVTFEDKKRENFERGNLELEKRRQALLEQQRKEQERLAQLERAEQERKERERQEQERKRQLELEKQLEKQRELERQREEERRKEIERREAAKRELERQRQLEWERNRRQELLNQRNKEQEDIVVLKAKKKTLEFELEALNDKKHQLEGKLQDIRCRLTTQRQEIESTNKSRELRIAEITHLQQQLQESQQMLGRLIPEKQILNDQLKQVQQNSLHRDSLLTLKRALEAKELARQQLRDQLDEVEKETRSKLQEIDIFNNQLKELREIHNKQQLQKQKSIEAERLKQKEQERKILELEKQKEEAQRRAQERDQQWLERVHQEDEQQRPRKPHEEEKLKREESVKKKDSEEKGKPEMQDKLSRLFHQHQEPAKPAVQAPWSTAEKGPLTISAQENVKVVYYRALYPFESRSHDEITIQPGDIVMVKGEWVDESQTGEPGWLGGELKGKTGWFPANYAEKIPENEVPAPVKPVTEAASTPTPKVAVRETPAPSSATPAEPSETPNNWADFSSTWPTSTNEKPETDSWDAWATQPSLTVPSAGQLRQRSAFTPATATGSSPSPVLGQGEKVEGLQAQALYPWRAKKDNHLNFNKNDVITVLEQQDMWWFGEVQGQKGWFPKSYVKLISGPIRKSTSMDSGSSESPASLKRVASPAAKPAVSGEEFIAMYTYESSEQGDLTFQQGDVILVTKKDGDWWTGTVGDKSGVFPSNYVRLKDSEGSGTAGKTGSLGKKPEIAQVIASYTATGPEQLTLAPGQLILIRKKNPGGWWEGELQARGKKRQIGWFPANYVKLLSPGTSKITPTDPPKPTAFPTVCQVIGMYDYTAQNDDELAFSKGQVINVLNKEDPDWWKGEVHGQVGLFPSNYVKLTTDTDPSQQWCSDLHLLDMLTPTERKRQGYIHELIVTEENYVNDLQLVTEIFQKPLMESELLTEKEGAMIFVNWKELIMCNIKLLKALRVRKKMSGEKMPVKMIGDILTAQLPHMQPYIRFCSCQLNGAALIQQKTDEAPDFKEFVKRLAMDPRCKGMPLSSFILKPMQRVTRYPLIIKNILENTPENHPDHSHLKHALEKAEELCSQVNEGVREKENSDRLEWIQAHVLCEGLSEQLVFNSVTNCLGPRKFLHSGKLYKAKSNKELYGFLFNDFLLLTQIIKPLGSSGTDKVFSPKSNLQYKMYKTPIFLNEVLVKLPTDPSGDEPIFHISHIDRVYTLRAESINERTAWVQKIKAASELYIETEKKKREKAYLVRSQRATGIGRLMVNVVEGIELKPCRSHGKSNPYCEVTMGSQCHITKTIQDTLNPKWNSNCQFFIRDLEQEVLCITVFERDQFSPDDFLGRTEIRVADIKKDQGSKGPVTKCLLLHEVPTGEIVVRLDLQLFDEP

Target ORF information:

RefSeq Version	XM_025281309.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis intersectin 1 (ITSN1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025281309.1

ORF Insert Sequence:

ATGGGTTTGA TCCCTCGGTC GGGCAGATGC CCTGGGGAAG GGAATAGCTA CCCACTCCAG 
TATTCTTGCC TGGAGAATCC CATGGACAGA AGAGCCTGGT GGAGCCCTAA AGTGAAGACA 
GTCTCAGAAA TGAGGGGACA TCTGGTCAGT CCAAGAGTTG AAACTGAAGA ACTTAACCCT 
GTTTTCTCTG GCAGCCTGGA TATTTGGGCC ATAACTGTAG AGGAAAGAGC AAAGCATGAT 
CAACAGTTCC ATAGTTTAAA GCCAATATCT GGATTTATTA CTGGTGATCA AGCTAGAAAC 
TTTTTTTTTC AATCTGGGTT ACCTCAGCCT GTTTTAGCAC AGATATGGGC GCTCGCTGAT 
ATGAATAATG ATGGAAGGAT GGATCAAGTG GAGTTTTCCA TTGCTATGAA ACTCATCAAG 
CTGAAGCTCC AAGGATATCA GCTCCCCTCT GCACTTCCCC CTGTCATGAA ACAGCAACCA 
GTTGCTATTT CTAGTGCACC AGCGTTTGGT ATGGGAGGTA TTGCCAGCAT GCCGCCGCTG 
ACCGCTGTTG CTCCTGTGCC AATGGGCTCC ATTCCCGTTG TTGGCATGTC TCCCCCCCTA 
GTATCATCTG TTCCTGCAGC AGGGGTGCCC CCCCTGGCCA ACGGGGCGCC CCCGGTCATA 
CAGCCTCTGC CGGCATTTGC TCATCCTGCT GCCACATTGC CAAAGAGTTC TTCCTTTAGT 
AGATCTGGCC CAGGGTCGCA GTTAAACACA AAATTACAGA AGGCACAATC ATTTGATGTG 
GCCAGCGTCC CTCCTACAGC TGAGTGGGCT GTTCCTCAGT CATCCAGGCT GAAATACAGA 
CAGCTCTTCA ATAGTCATGA CAAAACCATG AGTGGACACC TAACAGGTCC CCAAGCAAGA 
ACTATTCTTA TGCAATCAAG TTTACCACAG GCTCAGCTGG CTTCAATATG GAATCTTTCT 
GACATTGATC AAGATGGAAA ACTCACAGCA GAAGAATTTA TCCTGGCTAT GCACCTCATT 
GATGTAGCTA TGTCTGGTCA ACCATTGCCA CCGGTCCTGC CCCCAGAATA CATTCCACCT 
TCCTTTAGAA GAGTTCGATC TGGTAGTGGT ATATCTGTCA TAACCTCAAC TTCGGTGGAT 
CAGAGGTTGC CAGAGGAACC AGTTTTGGAA GATGAACAGC AACAACTAGA AAAGAAACTA 
CCTGTAACCT TTGAAGATAA GAAGCGGGAG AACTTTGAAC GGGGCAACCT GGAGCTGGAG 
AAGCGGAGAC AAGCGCTCTT GGAGCAGCAG CGTAAAGAGC AGGAGCGCCT GGCCCAGCTG 
GAGCGGGCAG AACAGGAGAG GAAGGAGCGG GAGCGCCAGG AGCAGGAGCG CAAAAGACAA 
CTGGAGCTGG AAAAGCAGCT AGAGAAGCAG CGGGAGCTGG AGCGGCAGAG GGAGGAGGAG 
CGAAGGAAGG AAATCGAGAG GCGGGAGGCT GCGAAACGGG AGCTTGAAAG GCAAAGACAG 
CTTGAATGGG AACGGAATCG AAGACAAGAA CTATTAAATC AAAGAAACAA AGAACAAGAA 
GACATAGTTG TACTGAAAGC AAAGAAAAAG ACTTTGGAAT TTGAATTAGA AGCTCTAAAT 
GATAAAAAAC ATCAATTAGA AGGAAAACTT CAGGATATCA GATGTCGACT GACAACCCAA 
AGGCAAGAAA TTGAGAGCAC AAACAAATCT AGAGAGTTGA GAATTGCAGA AATCACCCAC 
CTACAACAGC AATTACAGGA GTCCCAGCAA ATGCTTGGAA GACTTATTCC AGAAAAACAG 
ATACTCAATG ACCAATTGAA ACAAGTTCAG CAAAACAGTT TGCACAGGGA TTCACTTCTC 
ACACTCAAAA GAGCCTTAGA AGCGAAAGAA CTGGCTCGGC AGCAGCTACG AGACCAACTG 
GATGAAGTGG AGAAAGAAAC TCGGTCAAAA TTACAGGAGA TTGATATTTT CAACAATCAG 
CTGAAGGAAC TTAGAGAAAT ACATAATAAG CAGCAACTCC AGAAACAGAA GTCCATAGAG 
GCTGAACGAC TGAAACAGAA GGAACAAGAA CGAAAGATCC TGGAATTAGA AAAACAAAAA 
GAAGAAGCCC AAAGACGAGC TCAGGAAAGG GACCAGCAGT GGCTGGAGCG CGTGCATCAG 
GAGGATGAGC AGCAGCGGCC ACGAAAACCC CACGAGGAGG AGAAGCTCAA AAGGGAAGAG 
AGCGTCAAAA AGAAGGACAG TGAGGAGAAG GGCAAACCGG AAATGCAAGA CAAGCTGAGT 
CGGCTTTTCC ACCAGCATCA AGAACCAGCT AAGCCCGCTG TCCAGGCTCC CTGGTCCACT 
GCAGAAAAAG GCCCACTTAC GATTTCTGCA CAGGAGAATG TAAAAGTGGT GTATTACCGA 
GCACTTTATC CCTTTGAGTC CAGGAGTCAC GATGAAATCA CGATCCAGCC GGGAGATATA 
GTCATGGTTA AAGGGGAATG GGTGGACGAA AGCCAAACTG GAGAACCGGG ATGGCTTGGA 
GGAGAATTAA AAGGAAAGAC GGGATGGTTC CCTGCAAACT ACGCAGAGAA AATCCCAGAA 
AACGAGGTTC CCGCCCCAGT CAAACCAGTG ACTGAGGCGG CCTCCACCCC TACACCCAAA 
GTGGCTGTGC GTGAGACCCC CGCCCCTTCA TCTGCAACCC CTGCAGAGCC CTCCGAGACC 
CCCAACAACT GGGCCGACTT CAGCTCCACG TGGCCCACCA GCACGAATGA GAAACCAGAA 
ACCGACAGCT GGGACGCATG GGCAACCCAG CCCTCCCTCA CCGTGCCAAG CGCCGGGCAG 
CTGAGGCAGA GGTCGGCCTT CACTCCAGCC ACGGCCACGG GCTCATCTCC GTCTCCTGTT 
CTAGGCCAGG GTGAAAAGGT GGAGGGGCTA CAGGCGCAAG CCCTGTATCC GTGGAGAGCC 
AAGAAAGACA ACCATTTAAA TTTTAACAAA AACGATGTCA TCACCGTACT GGAGCAGCAA 
GACATGTGGT GGTTTGGAGA AGTTCAAGGC CAGAAGGGTT GGTTCCCCAA GTCCTACGTG 
AAACTCATTT CAGGGCCCAT AAGGAAATCG ACAAGCATGG ATTCTGGTTC CTCAGAAAGT 
CCTGCAAGTC TGAAGAGGGT AGCTTCCCCA GCAGCCAAGC CAGCTGTGTC GGGAGAAGAA 
TTTATTGCCA TGTACACTTA CGAGAGTTCT GAGCAAGGCG ATTTAACCTT TCAGCAAGGG 
GATGTGATTT TGGTTACCAA GAAAGATGGT GACTGGTGGA CAGGAACAGT GGGCGACAAG 
TCCGGCGTCT TCCCTTCTAA CTATGTGAGG CTTAAAGATT CAGAGGGCTC TGGAACTGCT 
GGGAAAACAG GGAGTTTAGG AAAAAAACCT GAAATCGCAC AGGTCATTGC CTCCTACACA 
GCTACAGGTC CTGAACAGCT TACCCTGGCC CCCGGTCAGC TGATCCTGAT CCGGAAAAAG 
AACCCAGGTG GATGGTGGGA AGGAGAGCTA CAGGCACGTG GGAAAAAGCG CCAGATAGGC 
TGGTTTCCTG CTAATTACGT CAAACTTTTA AGCCCTGGGA CGAGCAAAAT CACTCCAACA 
GACCCACCAA AGCCAACAGC TTTCCCAACA GTGTGCCAGG TGATCGGGAT GTACGACTAC 
ACCGCGCAGA ACGACGACGA GCTGGCCTTC AGCAAGGGCC AGGTCATCAA TGTCCTCAAC 
AAGGAGGACC CCGACTGGTG GAAGGGTGAA GTCCACGGGC AGGTGGGGCT CTTCCCGTCC 
AACTACGTGA AGCTGACCAC GGACACGGAC CCCAGCCAGC AATGGTGTTC AGACTTACAC 
CTCTTGGATA TGCTGACCCC AACTGAAAGA AAGCGCCAGG GGTACATCCA CGAGCTCATC 
GTCACAGAGG AGAACTATGT GAATGACCTG CAGCTGGTCA CAGAGATCTT TCAGAAACCC 
CTGATGGAGT CTGAGCTGCT GACAGAAAAA GAGGGTGCTA TGATTTTTGT TAACTGGAAG 
GAGCTGATTA TGTGTAATAT CAAACTACTG AAAGCGCTGA GGGTCCGCAA GAAGATGTCT 
GGGGAGAAGA TGCCCGTGAA GATGATCGGA GACATCCTGA CGGCCCAGCT GCCACACATG 
CAGCCCTACA TCCGCTTCTG CAGCTGCCAG CTCAACGGGG CCGCCTTGAT CCAGCAGAAG 
ACTGACGAGG CCCCAGACTT CAAGGAGTTT GTTAAAAGGC TGGCCATGGA CCCACGCTGT 
AAGGGAATGC CCCTGTCTAG TTTTATACTG AAGCCTATGC AACGAGTAAC GAGATACCCA 
CTGATCATTA AAAATATCCT GGAAAACACT CCCGAAAACC ACCCAGACCA CAGCCACCTG 
AAGCACGCCC TGGAGAAGGC AGAGGAGCTG TGCTCCCAGG TGAACGAAGG GGTGCGGGAG 
AAGGAGAACT CGGACCGACT GGAGTGGATC CAGGCCCACG TACTGTGTGA AGGCCTGTCA 
GAGCAACTTG TGTTCAATTC CGTGACCAAC TGCCTGGGGC CGCGCAAGTT CCTGCACAGT 
GGGAAGCTCT ACAAGGCCAA GAGCAACAAG GAGCTGTATG GCTTCCTCTT CAATGACTTC 
CTGCTGCTGA CCCAAATCAT CAAGCCCCTG GGCTCCTCAG GCACCGACAA AGTCTTCAGC 
CCCAAATCAA ACCTGCAGTA TAAAATGTAC AAAACACCTA TCTTCCTAAA TGAGGTTCTA 
GTGAAGTTGC CCACTGACCC TTCTGGGGAT GAACCCATCT TCCACATTTC CCACATCGAC 
CGAGTCTACA CCCTCCGAGC AGAGAGCATA AACGAAAGGA CTGCTTGGGT GCAGAAAATC 
AAAGCAGCTT CTGAACTCTA CATAGAGACA GAGAAGAAGA AGCGGGAGAA GGCGTATCTG 
GTCCGCTCCC AAAGAGCCAC AGGCATCGGA AGGTTGATGG TGAACGTGGT TGAAGGCATT 
GAGCTGAAAC CCTGTCGCTC CCATGGGAAG AGTAACCCAT ACTGCGAGGT GACCATGGGT 
TCCCAGTGTC ACATCACCAA GACGATCCAG GACACTCTGA ATCCCAAGTG GAATTCCAAC 
TGCCAGTTCT TCATCAGAGA CTTGGAGCAA GAGGTGCTGT GCATCACCGT GTTTGAGAGG 
GACCAGTTCT CGCCAGATGA TTTTTTGGGT CGGACGGAGA TCCGCGTGGC CGACATCAAG 
AAGGACCAGG GCTCCAAGGG TCCAGTTACG AAGTGTCTCC TGCTGCACGA AGTTCCCACG 
GGAGAGATCG TGGTCCGCTT GGACCTGCAG TTGTTCGATG AGCCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITSN1 ( XM_025281314.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025137099.1 Bubalus bubalis intersectin 1 (ITSN1), transcript variant X2, mRNA.

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CloneID	OBu42476
Clone ID Related Accession (Same CDS sequence)	XM_025281314.1
Accession Version	XM_025281314.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5313bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	intersectin-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037545.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTTTGA TCCCTCGGTC GGGCAGATGC CCTGGGGAAG GGAATAGCTA CCCACTCCAG 
TATTCTTGCC TGGAGAATCC CATGGACAGA AGAGCCTGGT GGAGCCCTAA AGTGAAGACA 
GTCTCAGAAA TGAGGGGACA TCTGGTCAGT CCAAGAGTTG AAACTGAAGA ACTTAACCCT 
GTTTTCTCTG GCAGCCTGGA TATTTGGGCC ATAACTGTAG AGGAAAGAGC AAAGCATGAT 
CAACAGTTCC ATAGTTTAAA GCCAATATCT GGATTTATTA CTGGTGATCA AGCTAGAAAC 
TTTTTTTTTC AATCTGGGTT ACCTCAGCCT GTTTTAGCAC AGATATGGGC GCTCGCTGAT 
ATGAATAATG ATGGAAGGAT GGATCAAGTG GAGTTTTCCA TTGCTATGAA ACTCATCAAG 
CTGAAGCTCC AAGGATATCA GCTCCCCTCT GCACTTCCCC CTGTCATGAA ACAGCAACCA 
GTTGCTATTT CTAGTGCACC AGCGTTTGGT ATGGGAGGTA TTGCCAGCAT GCCGCCGCTG 
ACCGCTGTTG CTCCTGTGCC AATGGGCTCC ATTCCCGTTG TTGGCATGTC TCCCCCCCTA 
GTATCATCTG TTCCTGCAGC AGGGGTGCCC CCCCTGGCCA ACGGGGCGCC CCCGGTCATA 
CAGCCTCTGC CGGCATTTGC TCATCCTGCT GCCACATTGC CAAAGAGTTC TTCCTTTAGT 
AGATCTGGCC CAGGGTCGCA GTTAAACACA AAATTACAGA AGGCACAATC ATTTGATGTG 
GCCAGCGTCC CTCCTACAGC TGAGTGGGCT GTTCCTCAGT CATCCAGGCT GAAATACAGA 
CAGCTCTTCA ATAGTCATGA CAAAACCATG AGTGGACACC TAACAGGTCC CCAAGCAAGA 
ACTATTCTTA TGCAATCAAG TTTACCACAG GCTCAGCTGG CTTCAATATG GAATCTTTCT 
GACATTGATC AAGATGGAAA ACTCACAGCA GAAGAATTTA TCCTGGCTAT GCACCTCATT 
GATGTAGCTA TGTCTGGTCA ACCATTGCCA CCGGTCCTGC CCCCAGAATA CATTCCACCT 
TCCTTTAGAA GAGTTCGATC TGGTAGTGGT ATATCTGTCA TAACCTCAAC TTCGGTGGAT 
CAGAGGTTGC CAGAGGAACC AGTTTTGGAA GATGAACAGC AACAACTAGA AAAGAAACTA 
CCTGTAACCT TTGAAGATAA GAAGCGGGAG AACTTTGAAC GGGGCAACCT GGAGCTGGAG 
AAGCGGAGAC AAGCGCTCTT GGAGCAGCAG CGTAAAGAGC AGGAGCGCCT GGCCCAGCTG 
GAGCGGGCAG AACAGGAGAG GAAGGAGCGG GAGCGCCAGG AGCAGGAGCG CAAAAGACAA 
CTGGAGCTGG AAAAGCAGCT AGAGAAGCAG CGGGAGCTGG AGCGGCAGAG GGAGGAGGAG 
CGAAGGAAGG AAATCGAGAG GCGGGAGGCT GCGAAACGGG AGCTTGAAAG GCAAAGACAG 
CTTGAATGGG AACGGAATCG AAGACAAGAA CTATTAAATC AAAGAAACAA AGAACAAGAA 
GACATAGTTG TACTGAAAGC AAAGAAAAAG ACTTTGGAAT TTGAATTAGA AGCTCTAAAT 
GATAAAAAAC ATCAATTAGA AGGAAAACTT CAGGATATCA GATGTCGACT GACAACCCAA 
AGGCAAGAAA TTGAGAGCAC AAACAAATCT AGAGAGTTGA GAATTGCAGA AATCACCCAC 
CTACAACAGC AATTACAGGA GTCCCAGCAA ATGCTTGGAA GACTTATTCC AGAAAAACAG 
ATACTCAATG ACCAATTGAA ACAAGTTCAG CAAAACAGTT TGCACAGGGA TTCACTTCTC 
ACACTCAAAA GAGCCTTAGA AGCGAAAGAA CTGGCTCGGC AGCAGCTACG AGACCAACTG 
GATGAAGTGG AGAAAGAAAC TCGGTCAAAA TTACAGGAGA TTGATATTTT CAACAATCAG 
CTGAAGGAAC TTAGAGAAAT ACATAATAAG CAGCAACTCC AGAAACAGAA GTCCATAGAG 
GCTGAACGAC TGAAACAGAA GGAACAAGAA CGAAAGATCC TGGAATTAGA AAAACAAAAA 
GAAGAAGCCC AAAGACGAGC TCAGGAAAGG GACCAGCAGT GGCTGGAGCG CGTGCATCAG 
GAGGATGAGC AGCAGCGGCC ACGAAAACCC CACGAGGAGG AGAAGCTCAA AAGGGAAGAG 
AGCGTCAAAA AGAAGGACAG TGAGGAGAAG GGCAAACCGG AAATGCAAGA CAAGCTGAGT 
CGGCTTTTCC ACCAGCATCA AGAACCAGCT AAGCCCGCTG TCCAGGCTCC CTGGTCCACT 
GCAGAAAAAG GCCCACTTAC GATTTCTGCA CAGGAGAATG TAAAAGTGGT GTATTACCGA 
GCACTTTATC CCTTTGAGTC CAGGAGTCAC GATGAAATCA CGATCCAGCC GGGAGATATA 
GTCATGGTGG ACGAAAGCCA AACTGGAGAA CCGGGATGGC TTGGAGGAGA ATTAAAAGGA 
AAGACGGGAT GGTTCCCTGC AAACTACGCA GAGAAAATCC CAGAAAACGA GGTTCCCGCC 
CCAGTCAAAC CAGTGACTGA GGCGGCCTCC ACCCCTACAC CCAAAGTGGC TGTGCGTGAG 
ACCCCCGCCC CTTCATCTGC AACCCCTGCA GAGCCCTCCG AGACCCCCAA CAACTGGGCC 
GACTTCAGCT CCACGTGGCC CACCAGCACG AATGAGAAAC CAGAAACCGA CAGCTGGGAC 
GCATGGGCAA CCCAGCCCTC CCTCACCGTG CCAAGCGCCG GGCAGCTGAG GCAGAGGTCG 
GCCTTCACTC CAGCCACGGC CACGGGCTCA TCTCCGTCTC CTGTTCTAGG CCAGGGTGAA 
AAGGTGGAGG GGCTACAGGC GCAAGCCCTG TATCCGTGGA GAGCCAAGAA AGACAACCAT 
TTAAATTTTA ACAAAAACGA TGTCATCACC GTACTGGAGC AGCAAGACAT GTGGTGGTTT 
GGAGAAGTTC AAGGCCAGAA GGGTTGGTTC CCCAAGTCCT ACGTGAAACT CATTTCAGGG 
CCCATAAGGA AATCGACAAG CATGGATTCT GGTTCCTCAG AAAGTCCTGC AAGTCTGAAG 
AGGGTAGCTT CCCCAGCAGC CAAGCCAGCT GTGTCGGGAG AAGAATTTAT TGCCATGTAC 
ACTTACGAGA GTTCTGAGCA AGGCGATTTA ACCTTTCAGC AAGGGGATGT GATTTTGGTT 
ACCAAGAAAG ATGGTGACTG GTGGACAGGA ACAGTGGGCG ACAAGTCCGG CGTCTTCCCT 
TCTAACTATG TGAGGCTTAA AGATTCAGAG GGCTCTGGAA CTGCTGGGAA AACAGGGAGT 
TTAGGAAAAA AACCTGAAAT CGCACAGGTC ATTGCCTCCT ACACAGCTAC AGGTCCTGAA 
CAGCTTACCC TGGCCCCCGG TCAGCTGATC CTGATCCGGA AAAAGAACCC AGGTGGATGG 
TGGGAAGGAG AGCTACAGGC ACGTGGGAAA AAGCGCCAGA TAGGCTGGTT TCCTGCTAAT 
TACGTCAAAC TTTTAAGCCC TGGGACGAGC AAAATCACTC CAACAGACCC ACCAAAGCCA 
ACAGCTTTCC CAACAGTGTG CCAGGTGATC GGGATGTACG ACTACACCGC GCAGAACGAC 
GACGAGCTGG CCTTCAGCAA GGGCCAGGTC ATCAATGTCC TCAACAAGGA GGACCCCGAC 
TGGTGGAAGG GTGAAGTCCA CGGGCAGGTG GGGCTCTTCC CGTCCAACTA CGTGAAGCTG 
ACCACGGACA CGGACCCCAG CCAGCAATGG TGTTCAGACT TACACCTCTT GGATATGCTG 
ACCCCAACTG AAAGAAAGCG CCAGGGGTAC ATCCACGAGC TCATCGTCAC AGAGGAGAAC 
TATGTGAATG ACCTGCAGCT GGTCACAGAG ATCTTTCAGA AACCCCTGAT GGAGTCTGAG 
CTGCTGACAG AAAAAGAGGG TGCTATGATT TTTGTTAACT GGAAGGAGCT GATTATGTGT 
AATATCAAAC TACTGAAAGC GCTGAGGGTC CGCAAGAAGA TGTCTGGGGA GAAGATGCCC 
GTGAAGATGA TCGGAGACAT CCTGACGGCC CAGCTGCCAC ACATGCAGCC CTACATCCGC 
TTCTGCAGCT GCCAGCTCAA CGGGGCCGCC TTGATCCAGC AGAAGACTGA CGAGGCCCCA 
GACTTCAAGG AGTTTGTTAA AAGGCTGGCC ATGGACCCAC GCTGTAAGGG AATGCCCCTG 
TCTAGTTTTA TACTGAAGCC TATGCAACGA GTAACGAGAT ACCCACTGAT CATTAAAAAT 
ATCCTGGAAA ACACTCCCGA AAACCACCCA GACCACAGCC ACCTGAAGCA CGCCCTGGAG 
AAGGCAGAGG AGCTGTGCTC CCAGGTGAAC GAAGGGGTGC GGGAGAAGGA GAACTCGGAC 
CGACTGGAGT GGATCCAGGC CCACGTACTG TGTGAAGGCC TGTCAGAGCA ACTTGTGTTC 
AATTCCGTGA CCAACTGCCT GGGGCCGCGC AAGTTCCTGC ACAGTGGGAA GCTCTACAAG 
GCCAAGAGCA ACAAGGAGCT GTATGGCTTC CTCTTCAATG ACTTCCTGCT GCTGACCCAA 
ATCATCAAGC CCCTGGGCTC CTCAGGCACC GACAAAGTCT TCAGCCCCAA ATCAAACCTG 
CAGTATAAAA TGTACAAAAC ACCTATCTTC CTAAATGAGG TTCTAGTGAA GTTGCCCACT 
GACCCTTCTG GGGATGAACC CATCTTCCAC ATTTCCCACA TCGACCGAGT CTACACCCTC 
CGAGCAGAGA GCATAAACGA AAGGACTGCT TGGGTGCAGA AAATCAAAGC AGCTTCTGAA 
CTCTACATAG AGACAGAGAA GAAGAAGCGG GAGAAGGCGT ATCTGGTCCG CTCCCAAAGA 
GCCACAGGCA TCGGAAGGTT GATGGTGAAC GTGGTTGAAG GCATTGAGCT GAAACCCTGT 
CGCTCCCATG GGAAGAGTAA CCCATACTGC GAGGTGACCA TGGGTTCCCA GTGTCACATC 
ACCAAGACGA TCCAGGACAC TCTGAATCCC AAGTGGAATT CCAACTGCCA GTTCTTCATC 
AGAGACTTGG AGCAAGAGGT GCTGTGCATC ACCGTGTTTG AGAGGGACCA GTTCTCGCCA 
GATGATTTTT TGGGTCGGAC GGAGATCCGC GTGGCCGACA TCAAGAAGGA CCAGGGCTCC 
AAGGGTCCAG TTACGAAGTG TCTCCTGCTG CACGAAGTTC CCACGGGAGA GATCGTGGTC 
CGCTTGGACC TGCAGTTGTT CGATGAGCCG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025137099.1
CDS	393..5705
Translation	MGLIPRSGRCPGEGNSYPLQYSCLENPMDRRAWWSPKVKTVSEMRGHLVSPRVETEELNPVFSGSLDIWAITVEERAKHDQQFHSLKPISGFITGDQARNFFFQSGLPQPVLAQIWALADMNNDGRMDQVEFSIAMKLIKLKLQGYQLPSALPPVMKQQPVAISSAPAFGMGGIASMPPLTAVAPVPMGSIPVVGMSPPLVSSVPAAGVPPLANGAPPVIQPLPAFAHPAATLPKSSSFSRSGPGSQLNTKLQKAQSFDVASVPPTAEWAVPQSSRLKYRQLFNSHDKTMSGHLTGPQARTILMQSSLPQAQLASIWNLSDIDQDGKLTAEEFILAMHLIDVAMSGQPLPPVLPPEYIPPSFRRVRSGSGISVITSTSVDQRLPEEPVLEDEQQQLEKKLPVTFEDKKRENFERGNLELEKRRQALLEQQRKEQERLAQLERAEQERKERERQEQERKRQLELEKQLEKQRELERQREEERRKEIERREAAKRELERQRQLEWERNRRQELLNQRNKEQEDIVVLKAKKKTLEFELEALNDKKHQLEGKLQDIRCRLTTQRQEIESTNKSRELRIAEITHLQQQLQESQQMLGRLIPEKQILNDQLKQVQQNSLHRDSLLTLKRALEAKELARQQLRDQLDEVEKETRSKLQEIDIFNNQLKELREIHNKQQLQKQKSIEAERLKQKEQERKILELEKQKEEAQRRAQERDQQWLERVHQEDEQQRPRKPHEEEKLKREESVKKKDSEEKGKPEMQDKLSRLFHQHQEPAKPAVQAPWSTAEKGPLTISAQENVKVVYYRALYPFESRSHDEITIQPGDIVMVDESQTGEPGWLGGELKGKTGWFPANYAEKIPENEVPAPVKPVTEAASTPTPKVAVRETPAPSSATPAEPSETPNNWADFSSTWPTSTNEKPETDSWDAWATQPSLTVPSAGQLRQRSAFTPATATGSSPSPVLGQGEKVEGLQAQALYPWRAKKDNHLNFNKNDVITVLEQQDMWWFGEVQGQKGWFPKSYVKLISGPIRKSTSMDSGSSESPASLKRVASPAAKPAVSGEEFIAMYTYESSEQGDLTFQQGDVILVTKKDGDWWTGTVGDKSGVFPSNYVRLKDSEGSGTAGKTGSLGKKPEIAQVIASYTATGPEQLTLAPGQLILIRKKNPGGWWEGELQARGKKRQIGWFPANYVKLLSPGTSKITPTDPPKPTAFPTVCQVIGMYDYTAQNDDELAFSKGQVINVLNKEDPDWWKGEVHGQVGLFPSNYVKLTTDTDPSQQWCSDLHLLDMLTPTERKRQGYIHELIVTEENYVNDLQLVTEIFQKPLMESELLTEKEGAMIFVNWKELIMCNIKLLKALRVRKKMSGEKMPVKMIGDILTAQLPHMQPYIRFCSCQLNGAALIQQKTDEAPDFKEFVKRLAMDPRCKGMPLSSFILKPMQRVTRYPLIIKNILENTPENHPDHSHLKHALEKAEELCSQVNEGVREKENSDRLEWIQAHVLCEGLSEQLVFNSVTNCLGPRKFLHSGKLYKAKSNKELYGFLFNDFLLLTQIIKPLGSSGTDKVFSPKSNLQYKMYKTPIFLNEVLVKLPTDPSGDEPIFHISHIDRVYTLRAESINERTAWVQKIKAASELYIETEKKKREKAYLVRSQRATGIGRLMVNVVEGIELKPCRSHGKSNPYCEVTMGSQCHITKTIQDTLNPKWNSNCQFFIRDLEQEVLCITVFERDQFSPDDFLGRTEIRVADIKKDQGSKGPVTKCLLLHEVPTGEIVVRLDLQLFDEP

Target ORF information:

RefSeq Version	XM_025281314.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis intersectin 1 (ITSN1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025281314.1

ORF Insert Sequence:

ATGGGTTTGA TCCCTCGGTC GGGCAGATGC CCTGGGGAAG GGAATAGCTA CCCACTCCAG 
TATTCTTGCC TGGAGAATCC CATGGACAGA AGAGCCTGGT GGAGCCCTAA AGTGAAGACA 
GTCTCAGAAA TGAGGGGACA TCTGGTCAGT CCAAGAGTTG AAACTGAAGA ACTTAACCCT 
GTTTTCTCTG GCAGCCTGGA TATTTGGGCC ATAACTGTAG AGGAAAGAGC AAAGCATGAT 
CAACAGTTCC ATAGTTTAAA GCCAATATCT GGATTTATTA CTGGTGATCA AGCTAGAAAC 
TTTTTTTTTC AATCTGGGTT ACCTCAGCCT GTTTTAGCAC AGATATGGGC GCTCGCTGAT 
ATGAATAATG ATGGAAGGAT GGATCAAGTG GAGTTTTCCA TTGCTATGAA ACTCATCAAG 
CTGAAGCTCC AAGGATATCA GCTCCCCTCT GCACTTCCCC CTGTCATGAA ACAGCAACCA 
GTTGCTATTT CTAGTGCACC AGCGTTTGGT ATGGGAGGTA TTGCCAGCAT GCCGCCGCTG 
ACCGCTGTTG CTCCTGTGCC AATGGGCTCC ATTCCCGTTG TTGGCATGTC TCCCCCCCTA 
GTATCATCTG TTCCTGCAGC AGGGGTGCCC CCCCTGGCCA ACGGGGCGCC CCCGGTCATA 
CAGCCTCTGC CGGCATTTGC TCATCCTGCT GCCACATTGC CAAAGAGTTC TTCCTTTAGT 
AGATCTGGCC CAGGGTCGCA GTTAAACACA AAATTACAGA AGGCACAATC ATTTGATGTG 
GCCAGCGTCC CTCCTACAGC TGAGTGGGCT GTTCCTCAGT CATCCAGGCT GAAATACAGA 
CAGCTCTTCA ATAGTCATGA CAAAACCATG AGTGGACACC TAACAGGTCC CCAAGCAAGA 
ACTATTCTTA TGCAATCAAG TTTACCACAG GCTCAGCTGG CTTCAATATG GAATCTTTCT 
GACATTGATC AAGATGGAAA ACTCACAGCA GAAGAATTTA TCCTGGCTAT GCACCTCATT 
GATGTAGCTA TGTCTGGTCA ACCATTGCCA CCGGTCCTGC CCCCAGAATA CATTCCACCT 
TCCTTTAGAA GAGTTCGATC TGGTAGTGGT ATATCTGTCA TAACCTCAAC TTCGGTGGAT 
CAGAGGTTGC CAGAGGAACC AGTTTTGGAA GATGAACAGC AACAACTAGA AAAGAAACTA 
CCTGTAACCT TTGAAGATAA GAAGCGGGAG AACTTTGAAC GGGGCAACCT GGAGCTGGAG 
AAGCGGAGAC AAGCGCTCTT GGAGCAGCAG CGTAAAGAGC AGGAGCGCCT GGCCCAGCTG 
GAGCGGGCAG AACAGGAGAG GAAGGAGCGG GAGCGCCAGG AGCAGGAGCG CAAAAGACAA 
CTGGAGCTGG AAAAGCAGCT AGAGAAGCAG CGGGAGCTGG AGCGGCAGAG GGAGGAGGAG 
CGAAGGAAGG AAATCGAGAG GCGGGAGGCT GCGAAACGGG AGCTTGAAAG GCAAAGACAG 
CTTGAATGGG AACGGAATCG AAGACAAGAA CTATTAAATC AAAGAAACAA AGAACAAGAA 
GACATAGTTG TACTGAAAGC AAAGAAAAAG ACTTTGGAAT TTGAATTAGA AGCTCTAAAT 
GATAAAAAAC ATCAATTAGA AGGAAAACTT CAGGATATCA GATGTCGACT GACAACCCAA 
AGGCAAGAAA TTGAGAGCAC AAACAAATCT AGAGAGTTGA GAATTGCAGA AATCACCCAC 
CTACAACAGC AATTACAGGA GTCCCAGCAA ATGCTTGGAA GACTTATTCC AGAAAAACAG 
ATACTCAATG ACCAATTGAA ACAAGTTCAG CAAAACAGTT TGCACAGGGA TTCACTTCTC 
ACACTCAAAA GAGCCTTAGA AGCGAAAGAA CTGGCTCGGC AGCAGCTACG AGACCAACTG 
GATGAAGTGG AGAAAGAAAC TCGGTCAAAA TTACAGGAGA TTGATATTTT CAACAATCAG 
CTGAAGGAAC TTAGAGAAAT ACATAATAAG CAGCAACTCC AGAAACAGAA GTCCATAGAG 
GCTGAACGAC TGAAACAGAA GGAACAAGAA CGAAAGATCC TGGAATTAGA AAAACAAAAA 
GAAGAAGCCC AAAGACGAGC TCAGGAAAGG GACCAGCAGT GGCTGGAGCG CGTGCATCAG 
GAGGATGAGC AGCAGCGGCC ACGAAAACCC CACGAGGAGG AGAAGCTCAA AAGGGAAGAG 
AGCGTCAAAA AGAAGGACAG TGAGGAGAAG GGCAAACCGG AAATGCAAGA CAAGCTGAGT 
CGGCTTTTCC ACCAGCATCA AGAACCAGCT AAGCCCGCTG TCCAGGCTCC CTGGTCCACT 
GCAGAAAAAG GCCCACTTAC GATTTCTGCA CAGGAGAATG TAAAAGTGGT GTATTACCGA 
GCACTTTATC CCTTTGAGTC CAGGAGTCAC GATGAAATCA CGATCCAGCC GGGAGATATA 
GTCATGGTGG ACGAAAGCCA AACTGGAGAA CCGGGATGGC TTGGAGGAGA ATTAAAAGGA 
AAGACGGGAT GGTTCCCTGC AAACTACGCA GAGAAAATCC CAGAAAACGA GGTTCCCGCC 
CCAGTCAAAC CAGTGACTGA GGCGGCCTCC ACCCCTACAC CCAAAGTGGC TGTGCGTGAG 
ACCCCCGCCC CTTCATCTGC AACCCCTGCA GAGCCCTCCG AGACCCCCAA CAACTGGGCC 
GACTTCAGCT CCACGTGGCC CACCAGCACG AATGAGAAAC CAGAAACCGA CAGCTGGGAC 
GCATGGGCAA CCCAGCCCTC CCTCACCGTG CCAAGCGCCG GGCAGCTGAG GCAGAGGTCG 
GCCTTCACTC CAGCCACGGC CACGGGCTCA TCTCCGTCTC CTGTTCTAGG CCAGGGTGAA 
AAGGTGGAGG GGCTACAGGC GCAAGCCCTG TATCCGTGGA GAGCCAAGAA AGACAACCAT 
TTAAATTTTA ACAAAAACGA TGTCATCACC GTACTGGAGC AGCAAGACAT GTGGTGGTTT 
GGAGAAGTTC AAGGCCAGAA GGGTTGGTTC CCCAAGTCCT ACGTGAAACT CATTTCAGGG 
CCCATAAGGA AATCGACAAG CATGGATTCT GGTTCCTCAG AAAGTCCTGC AAGTCTGAAG 
AGGGTAGCTT CCCCAGCAGC CAAGCCAGCT GTGTCGGGAG AAGAATTTAT TGCCATGTAC 
ACTTACGAGA GTTCTGAGCA AGGCGATTTA ACCTTTCAGC AAGGGGATGT GATTTTGGTT 
ACCAAGAAAG ATGGTGACTG GTGGACAGGA ACAGTGGGCG ACAAGTCCGG CGTCTTCCCT 
TCTAACTATG TGAGGCTTAA AGATTCAGAG GGCTCTGGAA CTGCTGGGAA AACAGGGAGT 
TTAGGAAAAA AACCTGAAAT CGCACAGGTC ATTGCCTCCT ACACAGCTAC AGGTCCTGAA 
CAGCTTACCC TGGCCCCCGG TCAGCTGATC CTGATCCGGA AAAAGAACCC AGGTGGATGG 
TGGGAAGGAG AGCTACAGGC ACGTGGGAAA AAGCGCCAGA TAGGCTGGTT TCCTGCTAAT 
TACGTCAAAC TTTTAAGCCC TGGGACGAGC AAAATCACTC CAACAGACCC ACCAAAGCCA 
ACAGCTTTCC CAACAGTGTG CCAGGTGATC GGGATGTACG ACTACACCGC GCAGAACGAC 
GACGAGCTGG CCTTCAGCAA GGGCCAGGTC ATCAATGTCC TCAACAAGGA GGACCCCGAC 
TGGTGGAAGG GTGAAGTCCA CGGGCAGGTG GGGCTCTTCC CGTCCAACTA CGTGAAGCTG 
ACCACGGACA CGGACCCCAG CCAGCAATGG TGTTCAGACT TACACCTCTT GGATATGCTG 
ACCCCAACTG AAAGAAAGCG CCAGGGGTAC ATCCACGAGC TCATCGTCAC AGAGGAGAAC 
TATGTGAATG ACCTGCAGCT GGTCACAGAG ATCTTTCAGA AACCCCTGAT GGAGTCTGAG 
CTGCTGACAG AAAAAGAGGG TGCTATGATT TTTGTTAACT GGAAGGAGCT GATTATGTGT 
AATATCAAAC TACTGAAAGC GCTGAGGGTC CGCAAGAAGA TGTCTGGGGA GAAGATGCCC 
GTGAAGATGA TCGGAGACAT CCTGACGGCC CAGCTGCCAC ACATGCAGCC CTACATCCGC 
TTCTGCAGCT GCCAGCTCAA CGGGGCCGCC TTGATCCAGC AGAAGACTGA CGAGGCCCCA 
GACTTCAAGG AGTTTGTTAA AAGGCTGGCC ATGGACCCAC GCTGTAAGGG AATGCCCCTG 
TCTAGTTTTA TACTGAAGCC TATGCAACGA GTAACGAGAT ACCCACTGAT CATTAAAAAT 
ATCCTGGAAA ACACTCCCGA AAACCACCCA GACCACAGCC ACCTGAAGCA CGCCCTGGAG 
AAGGCAGAGG AGCTGTGCTC CCAGGTGAAC GAAGGGGTGC GGGAGAAGGA GAACTCGGAC 
CGACTGGAGT GGATCCAGGC CCACGTACTG TGTGAAGGCC TGTCAGAGCA ACTTGTGTTC 
AATTCCGTGA CCAACTGCCT GGGGCCGCGC AAGTTCCTGC ACAGTGGGAA GCTCTACAAG 
GCCAAGAGCA ACAAGGAGCT GTATGGCTTC CTCTTCAATG ACTTCCTGCT GCTGACCCAA 
ATCATCAAGC CCCTGGGCTC CTCAGGCACC GACAAAGTCT TCAGCCCCAA ATCAAACCTG 
CAGTATAAAA TGTACAAAAC ACCTATCTTC CTAAATGAGG TTCTAGTGAA GTTGCCCACT 
GACCCTTCTG GGGATGAACC CATCTTCCAC ATTTCCCACA TCGACCGAGT CTACACCCTC 
CGAGCAGAGA GCATAAACGA AAGGACTGCT TGGGTGCAGA AAATCAAAGC AGCTTCTGAA 
CTCTACATAG AGACAGAGAA GAAGAAGCGG GAGAAGGCGT ATCTGGTCCG CTCCCAAAGA 
GCCACAGGCA TCGGAAGGTT GATGGTGAAC GTGGTTGAAG GCATTGAGCT GAAACCCTGT 
CGCTCCCATG GGAAGAGTAA CCCATACTGC GAGGTGACCA TGGGTTCCCA GTGTCACATC 
ACCAAGACGA TCCAGGACAC TCTGAATCCC AAGTGGAATT CCAACTGCCA GTTCTTCATC 
AGAGACTTGG AGCAAGAGGT GCTGTGCATC ACCGTGTTTG AGAGGGACCA GTTCTCGCCA 
GATGATTTTT TGGGTCGGAC GGAGATCCGC GTGGCCGACA TCAAGAAGGA CCAGGGCTCC 
AAGGGTCCAG TTACGAAGTG TCTCCTGCTG CACGAAGTTC CCACGGGAGA GATCGTGGTC 
CGCTTGGACC TGCAGTTGTT CGATGAGCCG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITSN1 ( XM_025281344.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025137129.1 Bubalus bubalis intersectin 1 (ITSN1), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OBu42477
Clone ID Related Accession (Same CDS sequence)	XM_025281344.1
Accession Version	XM_025281344.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5115bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	intersectin-1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037545.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTTTGA TCCCTCGGTC GGGCAGATGC CCTGGGGAAG GGAATAGCTA CCCACTCCAG 
TATTCTTGCC TGGAGAATCC CATGGACAGA AGAGCCTGGT GGAGCCCTAA AGTGAAGACA 
GTCTCAGAAA TGAGGGGACA TCTGGTCAGT CCAAGAGTTG AAACTGAAGA ACTTAACCCT 
GTTTTCTCTG GCAGCCTGGA TATTTGGGCC ATAACTGTAG AGGAAAGAGC AAAGCATGAT 
CAACAGTTCC ATAGTTTAAA GCCAATATCT GGATTTATTA CTGGTGATCA AGCTAGAAAC 
TTTTTTTTTC AATCTGGGTT ACCTCAGCCT GTTTTAGCAC AGATATGGGC GCTCGCTGAT 
ATGAATAATG ATGGAAGGAT GGATCAAGTG GAGTTTTCCA TTGCTATGAA ACTCATCAAG 
CTGAAGCTCC AAGGATATCA GCTCCCCTCT GCACTTCCCC CTGTCATGAA ACAGCAACCA 
GTTGCTATTT CTAGTGCACC AGCGTTTGGT ATGGGAGGTA TTGCCAGCAT GCCGCCGCTG 
ACCGCTGTTG CTCCTGTGCC AATGGGCTCC ATTCCCGTTG TTGGCATGTC TCCCCCCCTA 
GTATCATCTG TTCCTGCAGC AGGGGTGCCC CCCCTGGCCA ACGGGGCGCC CCCGGTCATA 
CAGCCTCTGC CGGCATTTGC TCATCCTGCT GCCACATTGC CAAAGAGTTC TTCCTTTAGT 
AGATCTGGCC CAGGGTCGCA GTTAAACACA AAATTACAGA AGGCACAATC ATTTGATGTG 
GCCAGCGTCC CTCCTACAGC TGAGTGGGCT GTTCCTCAGT CATCCAGGCT GAAATACAGA 
CAGCTCTTCA ATAGTCATGA CAAAACCATG AGTGGACACC TAACAGGTCC CCAAGCAAGA 
ACTATTCTTA TGCAATCAAG TTTACCACAG GCTCAGCTGG CTTCAATATG GAATCTTTCT 
GACATTGATC AAGATGGAAA ACTCACAGCA GAAGAATTTA TCCTGGCTAT GCACCTCATT 
GATGTAGCTA TGTCTGGTCA ACCATTGCCA CCGGTCCTGC CCCCAGAATA CATTCCACCT 
TCCTTTAGAA GAGTTCGATC TGGTAGTGGT ATATCTGTCA TAACCTCAAC TTCGGTGGAT 
CAGAGGTTGC CAGAGGAACC AGTTTTGGAA GATGAACAGC AACAACTAGA AAAGAAACTA 
CCTGTAACCT TTGAAGATAA GAAGCGGGAG AACTTTGAAC GGGGCAACCT GGAGCTGGAG 
AAGCGGAGAC AAGCGCTCTT GGAGCAGCAG CGTAAAGAGC AGGAGCGCCT GGCCCAGCTG 
GAGCGGGCAG AACAGGAGAG GAAGGAGCGG GAGCGCCAGG AGCAGGAGCG CAAAAGACAA 
CTGGAGCTGG AAAAGCAGCT AGAGAAGCAG CGGGAGCTGG AGCGGCAGAG GGAGGAGGAG 
CGAAGGAAGG AAATCGAGAG GCGGGAGGCT GCGAAACGGG AGCTTGAAAG GCAAAGACAG 
CTTGAATGGG AACGGAATCG AAGACAAGAA CTATTAAATC AAAGAAACAA AGAACAAGAA 
GACATAGTTG TACTGAAAGC AAAGAAAAAG ACTTTGGAAT TTGAATTAGA AGCTCTAAAT 
GATAAAAAAC ATCAATTAGA AGGAAAACTT CAGGATATCA GATGTCGACT GACAACCCAA 
AGGCAAGAAA TTGAGAGCAC AAACAAATCT AGAGAGTTGA GAATTGCAGA AATCACCCAC 
CTACAACAGC AATTACAGGA GTCCCAGCAA ATGCTTGGAA GACTTATTCC AGAAAAACAG 
ATACTCAATG ACCAATTGAA ACAAGTTCAG CAAAACAGTT TGCACAGGGA TTCACTTCTC 
ACACTCAAAA GAGCCTTAGA AGCGAAAGAA CTGGCTCGGC AGCAGCTACG AGACCAACTG 
GATGAAGTGG AGAAAGAAAC TCGGTCAAAA TTACAGGAGA TTGATATTTT CAACAATCAG 
CTGAAGGAAC TTAGAGAAAT ACATAATAAG CAGCAACTCC AGAAACAGAA GTCCATAGAG 
GCTGAACGAC TGAAACAGAA GGAACAAGAA CGAAAGATCC TGGAATTAGA AAAACAAAAA 
GAAGAAGCCC AAAGACGAGC TCAGGAAAGG GACCAGCAGT GGCTGGAGCG CGTGCATCAG 
GAGGATGAGC AGCAGCGGCC ACGAAAACCC CACGAGGAGG AGAAGCTCAA AAGGGAAGAG 
AGCGTCAAAA AGAAGGACAG TGAGGAGAAG GGCAAACCGG AAATGCAAGA CAAGCTGAGT 
CGGCTTTTCC ACCAGCATCA AGAACCAGCT AAGCCCGCTG TCCAGGCTCC CTGGTCCACT 
GCAGAAAAAG GCCCACTTAC GATTTCTGCA CAGGAGAATG TAAAAGTGGT GTATTACCGA 
GCACTTTATC CCTTTGAGTC CAGGAGTCAC GATGAAATCA CGATCCAGCC GGGAGATATA 
GTCATGGTTA AAGGGGAATG GGTGGACGAA AGCCAAACTG GAGAACCGGG ATGGCTTGGA 
GGAGAATTAA AAGGAAAGAC GGGATGGTTC CCTGCAAACT ACGCAGAGAA AATCCCAGAA 
AACGAGGTTC CCGCCCCAGT CAAACCAGTG ACTGAGGCGG CCTCCACCCC TACACCCAAA 
GTGGCTGTGC GTGAGACCCC CGCCCCTTCA TCTGCAACCC CTGCAGAGCC CTCCGAGACC 
CCCAACAACT GGGCCGACTT CAGCTCCACG TGGCCCACCA GCACGAATGA GAAACCAGAA 
ACCGACAGCT GGGACGCATG GGCAACCCAG CCCTCCCTCA CCGTGCCAAG CGCCGGGCAG 
CTGAGGCAGA GGTCGGCCTT CACTCCAGCC ACGGCCACGG GCTCATCTCC GTCTCCTGTT 
CTAGGCCAGG GTGAAAAGGT GGAGGGGCTA CAGGCGCAAG CCCTGTATCC GTGGAGAGCC 
AAGAAAGACA ACCATTTAAA TTTTAACAAA AACGATGTCA TCACCGTACT GGAGCAGCAA 
GACATGTGGT GGTTTGGAGA AGTTCAAGGC CAGAAGGGTT GGTTCCCCAA GTCCTACGTG 
AAACTCATTT CAGGGCCCAT AAGGAAATCG ACAAGCATGG ATTCTGGTTC CTCAGAAAGT 
CCTGCAAGTC TGAAGAGGGT AGCTTCCCCA GCAGCCAAGC CAGCTGTGTC GGGAGAAGAA 
ATCGCACAGG TCATTGCCTC CTACACAGCT ACAGGTCCTG AACAGCTTAC CCTGGCCCCC 
GGTCAGCTGA TCCTGATCCG GAAAAAGAAC CCAGGTGGAT GGTGGGAAGG AGAGCTACAG 
GCACGTGGGA AAAAGCGCCA GATAGGCTGG TTTCCTGCTA ATTACGTCAA ACTTTTAAGC 
CCTGGGACGA GCAAAATCAC TCCAACAGAC CCACCAAAGC CAACAGCTTT CCCAACAGTG 
TGCCAGGTGA TCGGGATGTA CGACTACACC GCGCAGAACG ACGACGAGCT GGCCTTCAGC 
AAGGGCCAGG TCATCAATGT CCTCAACAAG GAGGACCCCG ACTGGTGGAA GGGTGAAGTC 
CACGGGCAGG TGGGGCTCTT CCCGTCCAAC TACGTGAAGC TGACCACGGA CACGGACCCC 
AGCCAGCAAT GGTGTTCAGA CTTACACCTC TTGGATATGC TGACCCCAAC TGAAAGAAAG 
CGCCAGGGGT ACATCCACGA GCTCATCGTC ACAGAGGAGA ACTATGTGAA TGACCTGCAG 
CTGGTCACAG AGATCTTTCA GAAACCCCTG ATGGAGTCTG AGCTGCTGAC AGAAAAAGAG 
GGTGCTATGA TTTTTGTTAA CTGGAAGGAG CTGATTATGT GTAATATCAA ACTACTGAAA 
GCGCTGAGGG TCCGCAAGAA GATGTCTGGG GAGAAGATGC CCGTGAAGAT GATCGGAGAC 
ATCCTGACGG CCCAGCTGCC ACACATGCAG CCCTACATCC GCTTCTGCAG CTGCCAGCTC 
AACGGGGCCG CCTTGATCCA GCAGAAGACT GACGAGGCCC CAGACTTCAA GGAGTTTGTT 
AAAAGGCTGG CCATGGACCC ACGCTGTAAG GGAATGCCCC TGTCTAGTTT TATACTGAAG 
CCTATGCAAC GAGTAACGAG ATACCCACTG ATCATTAAAA ATATCCTGGA AAACACTCCC 
GAAAACCACC CAGACCACAG CCACCTGAAG CACGCCCTGG AGAAGGCAGA GGAGCTGTGC 
TCCCAGGTGA ACGAAGGGGT GCGGGAGAAG GAGAACTCGG ACCGACTGGA GTGGATCCAG 
GCCCACGTAC TGTGTGAAGG CCTGTCAGAG CAACTTGTGT TCAATTCCGT GACCAACTGC 
CTGGGGCCGC GCAAGTTCCT GCACAGTGGG AAGCTCTACA AGGCCAAGAG CAACAAGGAG 
CTGTATGGCT TCCTCTTCAA TGACTTCCTG CTGCTGACCC AAATCATCAA GCCCCTGGGC 
TCCTCAGGCA CCGACAAAGT CTTCAGCCCC AAATCAAACC TGCAGTATAA AATGTACAAA 
ACACCTATCT TCCTAAATGA GGTTCTAGTG AAGTTGCCCA CTGACCCTTC TGGGGATGAA 
CCCATCTTCC ACATTTCCCA CATCGACCGA GTCTACACCC TCCGAGCAGA GAGCATAAAC 
GAAAGGACTG CTTGGGTGCA GAAAATCAAA GCAGCTTCTG AACTCTACAT AGAGACAGAG 
AAGAAGAAGC GGGAGAAGGC GTATCTGGTC CGCTCCCAAA GAGCCACAGG CATCGGAAGG 
TTGATGGTGA ACGTGGTTGA AGGCATTGAG CTGAAACCCT GTCGCTCCCA TGGGAAGAGT 
AACCCATACT GCGAGGTGAC CATGGGTTCC CAGTGTCACA TCACCAAGAC GATCCAGGAC 
ACTCTGAATC CCAAGTGGAA TTCCAACTGC CAGTTCTTCA TCAGAGACTT GGAGCAAGAG 
GTGCTGTGCA TCACCGTGTT TGAGAGGGAC CAGTTCTCGC CAGATGATTT TTTGGGTCGG 
ACGGAGATCC GCGTGGCCGA CATCAAGAAG GACCAGGGCT CCAAGGGTCC AGTTACGAAG 
TGTCTCCTGC TGCACGAAGT TCCCACGGGA GAGATCGTGG TCCGCTTGGA CCTGCAGTTG 
TTCGATGAGC CGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025137129.1
CDS	395..5509
Translation	MGLIPRSGRCPGEGNSYPLQYSCLENPMDRRAWWSPKVKTVSEMRGHLVSPRVETEELNPVFSGSLDIWAITVEERAKHDQQFHSLKPISGFITGDQARNFFFQSGLPQPVLAQIWALADMNNDGRMDQVEFSIAMKLIKLKLQGYQLPSALPPVMKQQPVAISSAPAFGMGGIASMPPLTAVAPVPMGSIPVVGMSPPLVSSVPAAGVPPLANGAPPVIQPLPAFAHPAATLPKSSSFSRSGPGSQLNTKLQKAQSFDVASVPPTAEWAVPQSSRLKYRQLFNSHDKTMSGHLTGPQARTILMQSSLPQAQLASIWNLSDIDQDGKLTAEEFILAMHLIDVAMSGQPLPPVLPPEYIPPSFRRVRSGSGISVITSTSVDQRLPEEPVLEDEQQQLEKKLPVTFEDKKRENFERGNLELEKRRQALLEQQRKEQERLAQLERAEQERKERERQEQERKRQLELEKQLEKQRELERQREEERRKEIERREAAKRELERQRQLEWERNRRQELLNQRNKEQEDIVVLKAKKKTLEFELEALNDKKHQLEGKLQDIRCRLTTQRQEIESTNKSRELRIAEITHLQQQLQESQQMLGRLIPEKQILNDQLKQVQQNSLHRDSLLTLKRALEAKELARQQLRDQLDEVEKETRSKLQEIDIFNNQLKELREIHNKQQLQKQKSIEAERLKQKEQERKILELEKQKEEAQRRAQERDQQWLERVHQEDEQQRPRKPHEEEKLKREESVKKKDSEEKGKPEMQDKLSRLFHQHQEPAKPAVQAPWSTAEKGPLTISAQENVKVVYYRALYPFESRSHDEITIQPGDIVMVKGEWVDESQTGEPGWLGGELKGKTGWFPANYAEKIPENEVPAPVKPVTEAASTPTPKVAVRETPAPSSATPAEPSETPNNWADFSSTWPTSTNEKPETDSWDAWATQPSLTVPSAGQLRQRSAFTPATATGSSPSPVLGQGEKVEGLQAQALYPWRAKKDNHLNFNKNDVITVLEQQDMWWFGEVQGQKGWFPKSYVKLISGPIRKSTSMDSGSSESPASLKRVASPAAKPAVSGEEIAQVIASYTATGPEQLTLAPGQLILIRKKNPGGWWEGELQARGKKRQIGWFPANYVKLLSPGTSKITPTDPPKPTAFPTVCQVIGMYDYTAQNDDELAFSKGQVINVLNKEDPDWWKGEVHGQVGLFPSNYVKLTTDTDPSQQWCSDLHLLDMLTPTERKRQGYIHELIVTEENYVNDLQLVTEIFQKPLMESELLTEKEGAMIFVNWKELIMCNIKLLKALRVRKKMSGEKMPVKMIGDILTAQLPHMQPYIRFCSCQLNGAALIQQKTDEAPDFKEFVKRLAMDPRCKGMPLSSFILKPMQRVTRYPLIIKNILENTPENHPDHSHLKHALEKAEELCSQVNEGVREKENSDRLEWIQAHVLCEGLSEQLVFNSVTNCLGPRKFLHSGKLYKAKSNKELYGFLFNDFLLLTQIIKPLGSSGTDKVFSPKSNLQYKMYKTPIFLNEVLVKLPTDPSGDEPIFHISHIDRVYTLRAESINERTAWVQKIKAASELYIETEKKKREKAYLVRSQRATGIGRLMVNVVEGIELKPCRSHGKSNPYCEVTMGSQCHITKTIQDTLNPKWNSNCQFFIRDLEQEVLCITVFERDQFSPDDFLGRTEIRVADIKKDQGSKGPVTKCLLLHEVPTGEIVVRLDLQLFDEP

Target ORF information:

RefSeq Version	XM_025281344.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis intersectin 1 (ITSN1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025281344.1

ORF Insert Sequence:

ATGGGTTTGA TCCCTCGGTC GGGCAGATGC CCTGGGGAAG GGAATAGCTA CCCACTCCAG 
TATTCTTGCC TGGAGAATCC CATGGACAGA AGAGCCTGGT GGAGCCCTAA AGTGAAGACA 
GTCTCAGAAA TGAGGGGACA TCTGGTCAGT CCAAGAGTTG AAACTGAAGA ACTTAACCCT 
GTTTTCTCTG GCAGCCTGGA TATTTGGGCC ATAACTGTAG AGGAAAGAGC AAAGCATGAT 
CAACAGTTCC ATAGTTTAAA GCCAATATCT GGATTTATTA CTGGTGATCA AGCTAGAAAC 
TTTTTTTTTC AATCTGGGTT ACCTCAGCCT GTTTTAGCAC AGATATGGGC GCTCGCTGAT 
ATGAATAATG ATGGAAGGAT GGATCAAGTG GAGTTTTCCA TTGCTATGAA ACTCATCAAG 
CTGAAGCTCC AAGGATATCA GCTCCCCTCT GCACTTCCCC CTGTCATGAA ACAGCAACCA 
GTTGCTATTT CTAGTGCACC AGCGTTTGGT ATGGGAGGTA TTGCCAGCAT GCCGCCGCTG 
ACCGCTGTTG CTCCTGTGCC AATGGGCTCC ATTCCCGTTG TTGGCATGTC TCCCCCCCTA 
GTATCATCTG TTCCTGCAGC AGGGGTGCCC CCCCTGGCCA ACGGGGCGCC CCCGGTCATA 
CAGCCTCTGC CGGCATTTGC TCATCCTGCT GCCACATTGC CAAAGAGTTC TTCCTTTAGT 
AGATCTGGCC CAGGGTCGCA GTTAAACACA AAATTACAGA AGGCACAATC ATTTGATGTG 
GCCAGCGTCC CTCCTACAGC TGAGTGGGCT GTTCCTCAGT CATCCAGGCT GAAATACAGA 
CAGCTCTTCA ATAGTCATGA CAAAACCATG AGTGGACACC TAACAGGTCC CCAAGCAAGA 
ACTATTCTTA TGCAATCAAG TTTACCACAG GCTCAGCTGG CTTCAATATG GAATCTTTCT 
GACATTGATC AAGATGGAAA ACTCACAGCA GAAGAATTTA TCCTGGCTAT GCACCTCATT 
GATGTAGCTA TGTCTGGTCA ACCATTGCCA CCGGTCCTGC CCCCAGAATA CATTCCACCT 
TCCTTTAGAA GAGTTCGATC TGGTAGTGGT ATATCTGTCA TAACCTCAAC TTCGGTGGAT 
CAGAGGTTGC CAGAGGAACC AGTTTTGGAA GATGAACAGC AACAACTAGA AAAGAAACTA 
CCTGTAACCT TTGAAGATAA GAAGCGGGAG AACTTTGAAC GGGGCAACCT GGAGCTGGAG 
AAGCGGAGAC AAGCGCTCTT GGAGCAGCAG CGTAAAGAGC AGGAGCGCCT GGCCCAGCTG 
GAGCGGGCAG AACAGGAGAG GAAGGAGCGG GAGCGCCAGG AGCAGGAGCG CAAAAGACAA 
CTGGAGCTGG AAAAGCAGCT AGAGAAGCAG CGGGAGCTGG AGCGGCAGAG GGAGGAGGAG 
CGAAGGAAGG AAATCGAGAG GCGGGAGGCT GCGAAACGGG AGCTTGAAAG GCAAAGACAG 
CTTGAATGGG AACGGAATCG AAGACAAGAA CTATTAAATC AAAGAAACAA AGAACAAGAA 
GACATAGTTG TACTGAAAGC AAAGAAAAAG ACTTTGGAAT TTGAATTAGA AGCTCTAAAT 
GATAAAAAAC ATCAATTAGA AGGAAAACTT CAGGATATCA GATGTCGACT GACAACCCAA 
AGGCAAGAAA TTGAGAGCAC AAACAAATCT AGAGAGTTGA GAATTGCAGA AATCACCCAC 
CTACAACAGC AATTACAGGA GTCCCAGCAA ATGCTTGGAA GACTTATTCC AGAAAAACAG 
ATACTCAATG ACCAATTGAA ACAAGTTCAG CAAAACAGTT TGCACAGGGA TTCACTTCTC 
ACACTCAAAA GAGCCTTAGA AGCGAAAGAA CTGGCTCGGC AGCAGCTACG AGACCAACTG 
GATGAAGTGG AGAAAGAAAC TCGGTCAAAA TTACAGGAGA TTGATATTTT CAACAATCAG 
CTGAAGGAAC TTAGAGAAAT ACATAATAAG CAGCAACTCC AGAAACAGAA GTCCATAGAG 
GCTGAACGAC TGAAACAGAA GGAACAAGAA CGAAAGATCC TGGAATTAGA AAAACAAAAA 
GAAGAAGCCC AAAGACGAGC TCAGGAAAGG GACCAGCAGT GGCTGGAGCG CGTGCATCAG 
GAGGATGAGC AGCAGCGGCC ACGAAAACCC CACGAGGAGG AGAAGCTCAA AAGGGAAGAG 
AGCGTCAAAA AGAAGGACAG TGAGGAGAAG GGCAAACCGG AAATGCAAGA CAAGCTGAGT 
CGGCTTTTCC ACCAGCATCA AGAACCAGCT AAGCCCGCTG TCCAGGCTCC CTGGTCCACT 
GCAGAAAAAG GCCCACTTAC GATTTCTGCA CAGGAGAATG TAAAAGTGGT GTATTACCGA 
GCACTTTATC CCTTTGAGTC CAGGAGTCAC GATGAAATCA CGATCCAGCC GGGAGATATA 
GTCATGGTTA AAGGGGAATG GGTGGACGAA AGCCAAACTG GAGAACCGGG ATGGCTTGGA 
GGAGAATTAA AAGGAAAGAC GGGATGGTTC CCTGCAAACT ACGCAGAGAA AATCCCAGAA 
AACGAGGTTC CCGCCCCAGT CAAACCAGTG ACTGAGGCGG CCTCCACCCC TACACCCAAA 
GTGGCTGTGC GTGAGACCCC CGCCCCTTCA TCTGCAACCC CTGCAGAGCC CTCCGAGACC 
CCCAACAACT GGGCCGACTT CAGCTCCACG TGGCCCACCA GCACGAATGA GAAACCAGAA 
ACCGACAGCT GGGACGCATG GGCAACCCAG CCCTCCCTCA CCGTGCCAAG CGCCGGGCAG 
CTGAGGCAGA GGTCGGCCTT CACTCCAGCC ACGGCCACGG GCTCATCTCC GTCTCCTGTT 
CTAGGCCAGG GTGAAAAGGT GGAGGGGCTA CAGGCGCAAG CCCTGTATCC GTGGAGAGCC 
AAGAAAGACA ACCATTTAAA TTTTAACAAA AACGATGTCA TCACCGTACT GGAGCAGCAA 
GACATGTGGT GGTTTGGAGA AGTTCAAGGC CAGAAGGGTT GGTTCCCCAA GTCCTACGTG 
AAACTCATTT CAGGGCCCAT AAGGAAATCG ACAAGCATGG ATTCTGGTTC CTCAGAAAGT 
CCTGCAAGTC TGAAGAGGGT AGCTTCCCCA GCAGCCAAGC CAGCTGTGTC GGGAGAAGAA 
ATCGCACAGG TCATTGCCTC CTACACAGCT ACAGGTCCTG AACAGCTTAC CCTGGCCCCC 
GGTCAGCTGA TCCTGATCCG GAAAAAGAAC CCAGGTGGAT GGTGGGAAGG AGAGCTACAG 
GCACGTGGGA AAAAGCGCCA GATAGGCTGG TTTCCTGCTA ATTACGTCAA ACTTTTAAGC 
CCTGGGACGA GCAAAATCAC TCCAACAGAC CCACCAAAGC CAACAGCTTT CCCAACAGTG 
TGCCAGGTGA TCGGGATGTA CGACTACACC GCGCAGAACG ACGACGAGCT GGCCTTCAGC 
AAGGGCCAGG TCATCAATGT CCTCAACAAG GAGGACCCCG ACTGGTGGAA GGGTGAAGTC 
CACGGGCAGG TGGGGCTCTT CCCGTCCAAC TACGTGAAGC TGACCACGGA CACGGACCCC 
AGCCAGCAAT GGTGTTCAGA CTTACACCTC TTGGATATGC TGACCCCAAC TGAAAGAAAG 
CGCCAGGGGT ACATCCACGA GCTCATCGTC ACAGAGGAGA ACTATGTGAA TGACCTGCAG 
CTGGTCACAG AGATCTTTCA GAAACCCCTG ATGGAGTCTG AGCTGCTGAC AGAAAAAGAG 
GGTGCTATGA TTTTTGTTAA CTGGAAGGAG CTGATTATGT GTAATATCAA ACTACTGAAA 
GCGCTGAGGG TCCGCAAGAA GATGTCTGGG GAGAAGATGC CCGTGAAGAT GATCGGAGAC 
ATCCTGACGG CCCAGCTGCC ACACATGCAG CCCTACATCC GCTTCTGCAG CTGCCAGCTC 
AACGGGGCCG CCTTGATCCA GCAGAAGACT GACGAGGCCC CAGACTTCAA GGAGTTTGTT 
AAAAGGCTGG CCATGGACCC ACGCTGTAAG GGAATGCCCC TGTCTAGTTT TATACTGAAG 
CCTATGCAAC GAGTAACGAG ATACCCACTG ATCATTAAAA ATATCCTGGA AAACACTCCC 
GAAAACCACC CAGACCACAG CCACCTGAAG CACGCCCTGG AGAAGGCAGA GGAGCTGTGC 
TCCCAGGTGA ACGAAGGGGT GCGGGAGAAG GAGAACTCGG ACCGACTGGA GTGGATCCAG 
GCCCACGTAC TGTGTGAAGG CCTGTCAGAG CAACTTGTGT TCAATTCCGT GACCAACTGC 
CTGGGGCCGC GCAAGTTCCT GCACAGTGGG AAGCTCTACA AGGCCAAGAG CAACAAGGAG 
CTGTATGGCT TCCTCTTCAA TGACTTCCTG CTGCTGACCC AAATCATCAA GCCCCTGGGC 
TCCTCAGGCA CCGACAAAGT CTTCAGCCCC AAATCAAACC TGCAGTATAA AATGTACAAA 
ACACCTATCT TCCTAAATGA GGTTCTAGTG AAGTTGCCCA CTGACCCTTC TGGGGATGAA 
CCCATCTTCC ACATTTCCCA CATCGACCGA GTCTACACCC TCCGAGCAGA GAGCATAAAC 
GAAAGGACTG CTTGGGTGCA GAAAATCAAA GCAGCTTCTG AACTCTACAT AGAGACAGAG 
AAGAAGAAGC GGGAGAAGGC GTATCTGGTC CGCTCCCAAA GAGCCACAGG CATCGGAAGG 
TTGATGGTGA ACGTGGTTGA AGGCATTGAG CTGAAACCCT GTCGCTCCCA TGGGAAGAGT 
AACCCATACT GCGAGGTGAC CATGGGTTCC CAGTGTCACA TCACCAAGAC GATCCAGGAC 
ACTCTGAATC CCAAGTGGAA TTCCAACTGC CAGTTCTTCA TCAGAGACTT GGAGCAAGAG 
GTGCTGTGCA TCACCGTGTT TGAGAGGGAC CAGTTCTCGC CAGATGATTT TTTGGGTCGG 
ACGGAGATCC GCGTGGCCGA CATCAAGAAG GACCAGGGCT CCAAGGGTCC AGTTACGAAG 
TGTCTCCTGC TGCACGAAGT TCCCACGGGA GAGATCGTGG TCCGCTTGGA CCTGCAGTTG 
TTCGATGAGC CGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITSN1 ( XM_025281349.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025137134.1 Bubalus bubalis intersectin 1 (ITSN1), transcript variant X9, mRNA.

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CloneID	OBu42478
Clone ID Related Accession (Same CDS sequence)	XM_025281349.1
Accession Version	XM_025281349.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4953bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	intersectin-1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037545.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCAGT TTCCCACACC TTTTGGTGGC AGCCTGGATA TTTGGGCCAT AACTGTAGAG 
GAAAGAGCAA AGCATGATCA ACAGTTCCAT AGTTTAAAGC CAATATCTGG ATTTATTACT 
GGTGATCAAG CTAGAAACTT TTTTTTTCAA TCTGGGTTAC CTCAGCCTGT TTTAGCACAG 
ATATGGGCGC TCGCTGATAT GAATAATGAT GGAAGGATGG ATCAAGTGGA GTTTTCCATT 
GCTATGAAAC TCATCAAGCT GAAGCTCCAA GGATATCAGC TCCCCTCTGC ACTTCCCCCT 
GTCATGAAAC AGCAACCAGT TGCTATTTCT AGTGCACCAG CGTTTGGTAT GGGAGGTATT 
GCCAGCATGC CGCCGCTGAC CGCTGTTGCT CCTGTGCCAA TGGGCTCCAT TCCCGTTGTT 
GGCATGTCTC CCCCCCTAGT ATCATCTGTT CCTGCAGCAG GGGTGCCCCC CCTGGCCAAC 
GGGGCGCCCC CGGTCATACA GCCTCTGCCG GCATTTGCTC ATCCTGCTGC CACATTGCCA 
AAGAGTTCTT CCTTTAGTAG ATCTGGCCCA GGGTCGCAGT TAAACACAAA ATTACAGAAG 
GCACAATCAT TTGATGTGGC CAGCGTCCCT CCTACAGCTG AGTGGGCTGT TCCTCAGTCA 
TCCAGGCTGA AATACAGACA GCTCTTCAAT AGTCATGACA AAACCATGAG TGGACACCTA 
ACAGGTCCCC AAGCAAGAAC TATTCTTATG CAATCAAGTT TACCACAGGC TCAGCTGGCT 
TCAATATGGA ATCTTTCTGA CATTGATCAA GATGGAAAAC TCACAGCAGA AGAATTTATC 
CTGGCTATGC ACCTCATTGA TGTAGCTATG TCTGGTCAAC CATTGCCACC GGTCCTGCCC 
CCAGAATACA TTCCACCTTC CTTTAGAAGA GTTCGATCTG GTAGTGGTAT ATCTGTCATA 
ACCTCAACTT CGGTGGATCA GAGGTTGCCA GAGGAACCAG TTTTGGAAGA TGAACAGCAA 
CAACTAGAAA AGAAACTACC TGTAACCTTT GAAGATAAGA AGCGGGAGAA CTTTGAACGG 
GGCAACCTGG AGCTGGAGAA GCGGAGACAA GCGCTCTTGG AGCAGCAGCG TAAAGAGCAG 
GAGCGCCTGG CCCAGCTGGA GCGGGCAGAA CAGGAGAGGA AGGAGCGGGA GCGCCAGGAG 
CAGGAGCGCA AAAGACAACT GGAGCTGGAA AAGCAGCTAG AGAAGCAGCG GGAGCTGGAG 
CGGCAGAGGG AGGAGGAGCG AAGGAAGGAA ATCGAGAGGC GGGAGGCTGC GAAACGGGAG 
CTTGAAAGGC AAAGACAGCT TGAATGGGAA CGGAATCGAA GACAAGAACT ATTAAATCAA 
AGAAACAAAG AACAAGAAGA CATAGTTGTA CTGAAAGCAA AGAAAAAGAC TTTGGAATTT 
GAATTAGAAG CTCTAAATGA TAAAAAACAT CAATTAGAAG GAAAACTTCA GGATATCAGA 
TGTCGACTGA CAACCCAAAG GCAAGAAATT GAGAGCACAA ACAAATCTAG AGAGTTGAGA 
ATTGCAGAAA TCACCCACCT ACAACAGCAA TTACAGGAGT CCCAGCAAAT GCTTGGAAGA 
CTTATTCCAG AAAAACAGAT ACTCAATGAC CAATTGAAAC AAGTTCAGCA AAACAGTTTG 
CACAGGGATT CACTTCTCAC ACTCAAAAGA GCCTTAGAAG CGAAAGAACT GGCTCGGCAG 
CAGCTACGAG ACCAACTGGA TGAAGTGGAG AAAGAAACTC GGTCAAAATT ACAGGAGATT 
GATATTTTCA ACAATCAGCT GAAGGAACTT AGAGAAATAC ATAATAAGCA GCAACTCCAG 
AAACAGAAGT CCATAGAGGC TGAACGACTG AAACAGAAGG AACAAGAACG AAAGATCCTG 
GAATTAGAAA AACAAAAAGA AGAAGCCCAA AGACGAGCTC AGGAAAGGGA CCAGCAGTGG 
CTGGAGCGCG TGCATCAGGA GGATGAGCAG CAGCGGCCAC GAAAACCCCA CGAGGAGGAG 
AAGCTCAAAA GGGAAGAGAG CGTCAAAAAG AAGGACAGTG AGGAGAAGGG CAAACCGGAA 
ATGCAAGACA AGCTGAGTCG GCTTTTCCAC CAGCATCAAG AACCAGCTAA GCCCGCTGTC 
CAGGCTCCCT GGTCCACTGC AGAAAAAGGC CCACTTACGA TTTCTGCACA GGAGAATGTA 
AAAGTGGTGT ATTACCGAGC ACTTTATCCC TTTGAGTCCA GGAGTCACGA TGAAATCACG 
ATCCAGCCGG GAGATATAGT CATGGTTAAA GGGGAATGGG TGGACGAAAG CCAAACTGGA 
GAACCGGGAT GGCTTGGAGG AGAATTAAAA GGAAAGACGG GATGGTTCCC TGCAAACTAC 
GCAGAGAAAA TCCCAGAAAA CGAGGTTCCC GCCCCAGTCA AACCAGTGAC TGAGGCGGCC 
TCCACCCCTA CACCCAAAGT GGCTGTGCGT GAGACCCCCG CCCCTTCATC TGCAACCCCT 
GCAGAGCCCT CCGAGACCCC CAACAACTGG GCCGACTTCA GCTCCACGTG GCCCACCAGC 
ACGAATGAGA AACCAGAAAC CGACAGCTGG GACGCATGGG CAACCCAGCC CTCCCTCACC 
GTGCCAAGCG CCGGGCAGCT GAGGCAGAGG TCGGCCTTCA CTCCAGCCAC GGCCACGGGC 
TCATCTCCGT CTCCTGTTCT AGGCCAGGGT GAAAAGGTGG AGGGGCTACA GGCGCAAGCC 
CTGTATCCGT GGAGAGCCAA GAAAGACAAC CATTTAAATT TTAACAAAAA CGATGTCATC 
ACCGTACTGG AGCAGCAAGA CATGTGGTGG TTTGGAGAAG TTCAAGGCCA GAAGGGTTGG 
TTCCCCAAGT CCTACGTGAA ACTCATTTCA GGGCCCATAA GGAAATCGAC AAGCATGGAT 
TCTGGTTCCT CAGAAAGTCC TGCAAGTCTG AAGAGGGTAG CTTCCCCAGC AGCCAAGCCA 
GCTGTGTCGG GAGAAGAAAT CGCACAGGTC ATTGCCTCCT ACACAGCTAC AGGTCCTGAA 
CAGCTTACCC TGGCCCCCGG TCAGCTGATC CTGATCCGGA AAAAGAACCC AGGTGGATGG 
TGGGAAGGAG AGCTACAGGC ACGTGGGAAA AAGCGCCAGA TAGGCTGGTT TCCTGCTAAT 
TACGTCAAAC TTTTAAGCCC TGGGACGAGC AAAATCACTC CAACAGACCC ACCAAAGCCA 
ACAGCTTTCC CAACAGTGTG CCAGGTGATC GGGATGTACG ACTACACCGC GCAGAACGAC 
GACGAGCTGG CCTTCAGCAA GGGCCAGGTC ATCAATGTCC TCAACAAGGA GGACCCCGAC 
TGGTGGAAGG GTGAAGTCCA CGGGCAGGTG GGGCTCTTCC CGTCCAACTA CGTGAAGCTG 
ACCACGGACA CGGACCCCAG CCAGCAATGG TGTTCAGACT TACACCTCTT GGATATGCTG 
ACCCCAACTG AAAGAAAGCG CCAGGGGTAC ATCCACGAGC TCATCGTCAC AGAGGAGAAC 
TATGTGAATG ACCTGCAGCT GGTCACAGAG ATCTTTCAGA AACCCCTGAT GGAGTCTGAG 
CTGCTGACAG AAAAAGAGGG TGCTATGATT TTTGTTAACT GGAAGGAGCT GATTATGTGT 
AATATCAAAC TACTGAAAGC GCTGAGGGTC CGCAAGAAGA TGTCTGGGGA GAAGATGCCC 
GTGAAGATGA TCGGAGACAT CCTGACGGCC CAGCTGCCAC ACATGCAGCC CTACATCCGC 
TTCTGCAGCT GCCAGCTCAA CGGGGCCGCC TTGATCCAGC AGAAGACTGA CGAGGCCCCA 
GACTTCAAGG AGTTTGTTAA AAGGCTGGCC ATGGACCCAC GCTGTAAGGG AATGCCCCTG 
TCTAGTTTTA TACTGAAGCC TATGCAACGA GTAACGAGAT ACCCACTGAT CATTAAAAAT 
ATCCTGGAAA ACACTCCCGA AAACCACCCA GACCACAGCC ACCTGAAGCA CGCCCTGGAG 
AAGGCAGAGG AGCTGTGCTC CCAGGTGAAC GAAGGGGTGC GGGAGAAGGA GAACTCGGAC 
CGACTGGAGT GGATCCAGGC CCACGTACTG TGTGAAGGCC TGTCAGAGCA ACTTGTGTTC 
AATTCCGTGA CCAACTGCCT GGGGCCGCGC AAGTTCCTGC ACAGTGGGAA GCTCTACAAG 
GCCAAGAGCA ACAAGGAGCT GTATGGCTTC CTCTTCAATG ACTTCCTGCT GCTGACCCAA 
ATCATCAAGC CCCTGGGCTC CTCAGGCACC GACAAAGTCT TCAGCCCCAA ATCAAACCTG 
CAGTATAAAA TGTACAAAAC ACCTATCTTC CTAAATGAGG TTCTAGTGAA GTTGCCCACT 
GACCCTTCTG GGGATGAACC CATCTTCCAC ATTTCCCACA TCGACCGAGT CTACACCCTC 
CGAGCAGAGA GCATAAACGA AAGGACTGCT TGGGTGCAGA AAATCAAAGC AGCTTCTGAA 
CTCTACATAG AGACAGAGAA GAAGAAGCGG GAGAAGGCGT ATCTGGTCCG CTCCCAAAGA 
GCCACAGGCA TCGGAAGGTT GATGGTGAAC GTGGTTGAAG GCATTGAGCT GAAACCCTGT 
CGCTCCCATG GGAAGAGTAA CCCATACTGC GAGGTGACCA TGGGTTCCCA GTGTCACATC 
ACCAAGACGA TCCAGGACAC TCTGAATCCC AAGTGGAATT CCAACTGCCA GTTCTTCATC 
AGAGACTTGG AGCAAGAGGT GCTGTGCATC ACCGTGTTTG AGAGGGACCA GTTCTCGCCA 
GATGATTTTT TGGGTCGGAC GGAGATCCGC GTGGCCGACA TCAAGAAGGA CCAGGGCTCC 
AAGGGTCCAG TTACGAAGTG TCTCCTGCTG CACGAAGTTC CCACGGGAGA GATCGTGGTC 
CGCTTGGACC TGCAGTTGTT CGATGAGCCG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025137134.1
CDS	302..5254
Translation	MAQFPTPFGGSLDIWAITVEERAKHDQQFHSLKPISGFITGDQARNFFFQSGLPQPVLAQIWALADMNNDGRMDQVEFSIAMKLIKLKLQGYQLPSALPPVMKQQPVAISSAPAFGMGGIASMPPLTAVAPVPMGSIPVVGMSPPLVSSVPAAGVPPLANGAPPVIQPLPAFAHPAATLPKSSSFSRSGPGSQLNTKLQKAQSFDVASVPPTAEWAVPQSSRLKYRQLFNSHDKTMSGHLTGPQARTILMQSSLPQAQLASIWNLSDIDQDGKLTAEEFILAMHLIDVAMSGQPLPPVLPPEYIPPSFRRVRSGSGISVITSTSVDQRLPEEPVLEDEQQQLEKKLPVTFEDKKRENFERGNLELEKRRQALLEQQRKEQERLAQLERAEQERKERERQEQERKRQLELEKQLEKQRELERQREEERRKEIERREAAKRELERQRQLEWERNRRQELLNQRNKEQEDIVVLKAKKKTLEFELEALNDKKHQLEGKLQDIRCRLTTQRQEIESTNKSRELRIAEITHLQQQLQESQQMLGRLIPEKQILNDQLKQVQQNSLHRDSLLTLKRALEAKELARQQLRDQLDEVEKETRSKLQEIDIFNNQLKELREIHNKQQLQKQKSIEAERLKQKEQERKILELEKQKEEAQRRAQERDQQWLERVHQEDEQQRPRKPHEEEKLKREESVKKKDSEEKGKPEMQDKLSRLFHQHQEPAKPAVQAPWSTAEKGPLTISAQENVKVVYYRALYPFESRSHDEITIQPGDIVMVKGEWVDESQTGEPGWLGGELKGKTGWFPANYAEKIPENEVPAPVKPVTEAASTPTPKVAVRETPAPSSATPAEPSETPNNWADFSSTWPTSTNEKPETDSWDAWATQPSLTVPSAGQLRQRSAFTPATATGSSPSPVLGQGEKVEGLQAQALYPWRAKKDNHLNFNKNDVITVLEQQDMWWFGEVQGQKGWFPKSYVKLISGPIRKSTSMDSGSSESPASLKRVASPAAKPAVSGEEIAQVIASYTATGPEQLTLAPGQLILIRKKNPGGWWEGELQARGKKRQIGWFPANYVKLLSPGTSKITPTDPPKPTAFPTVCQVIGMYDYTAQNDDELAFSKGQVINVLNKEDPDWWKGEVHGQVGLFPSNYVKLTTDTDPSQQWCSDLHLLDMLTPTERKRQGYIHELIVTEENYVNDLQLVTEIFQKPLMESELLTEKEGAMIFVNWKELIMCNIKLLKALRVRKKMSGEKMPVKMIGDILTAQLPHMQPYIRFCSCQLNGAALIQQKTDEAPDFKEFVKRLAMDPRCKGMPLSSFILKPMQRVTRYPLIIKNILENTPENHPDHSHLKHALEKAEELCSQVNEGVREKENSDRLEWIQAHVLCEGLSEQLVFNSVTNCLGPRKFLHSGKLYKAKSNKELYGFLFNDFLLLTQIIKPLGSSGTDKVFSPKSNLQYKMYKTPIFLNEVLVKLPTDPSGDEPIFHISHIDRVYTLRAESINERTAWVQKIKAASELYIETEKKKREKAYLVRSQRATGIGRLMVNVVEGIELKPCRSHGKSNPYCEVTMGSQCHITKTIQDTLNPKWNSNCQFFIRDLEQEVLCITVFERDQFSPDDFLGRTEIRVADIKKDQGSKGPVTKCLLLHEVPTGEIVVRLDLQLFDEP

Target ORF information:

RefSeq Version	XM_025281349.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis intersectin 1 (ITSN1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025281349.1

ORF Insert Sequence:

ATGGCTCAGT TTCCCACACC TTTTGGTGGC AGCCTGGATA TTTGGGCCAT AACTGTAGAG 
GAAAGAGCAA AGCATGATCA ACAGTTCCAT AGTTTAAAGC CAATATCTGG ATTTATTACT 
GGTGATCAAG CTAGAAACTT TTTTTTTCAA TCTGGGTTAC CTCAGCCTGT TTTAGCACAG 
ATATGGGCGC TCGCTGATAT GAATAATGAT GGAAGGATGG ATCAAGTGGA GTTTTCCATT 
GCTATGAAAC TCATCAAGCT GAAGCTCCAA GGATATCAGC TCCCCTCTGC ACTTCCCCCT 
GTCATGAAAC AGCAACCAGT TGCTATTTCT AGTGCACCAG CGTTTGGTAT GGGAGGTATT 
GCCAGCATGC CGCCGCTGAC CGCTGTTGCT CCTGTGCCAA TGGGCTCCAT TCCCGTTGTT 
GGCATGTCTC CCCCCCTAGT ATCATCTGTT CCTGCAGCAG GGGTGCCCCC CCTGGCCAAC 
GGGGCGCCCC CGGTCATACA GCCTCTGCCG GCATTTGCTC ATCCTGCTGC CACATTGCCA 
AAGAGTTCTT CCTTTAGTAG ATCTGGCCCA GGGTCGCAGT TAAACACAAA ATTACAGAAG 
GCACAATCAT TTGATGTGGC CAGCGTCCCT CCTACAGCTG AGTGGGCTGT TCCTCAGTCA 
TCCAGGCTGA AATACAGACA GCTCTTCAAT AGTCATGACA AAACCATGAG TGGACACCTA 
ACAGGTCCCC AAGCAAGAAC TATTCTTATG CAATCAAGTT TACCACAGGC TCAGCTGGCT 
TCAATATGGA ATCTTTCTGA CATTGATCAA GATGGAAAAC TCACAGCAGA AGAATTTATC 
CTGGCTATGC ACCTCATTGA TGTAGCTATG TCTGGTCAAC CATTGCCACC GGTCCTGCCC 
CCAGAATACA TTCCACCTTC CTTTAGAAGA GTTCGATCTG GTAGTGGTAT ATCTGTCATA 
ACCTCAACTT CGGTGGATCA GAGGTTGCCA GAGGAACCAG TTTTGGAAGA TGAACAGCAA 
CAACTAGAAA AGAAACTACC TGTAACCTTT GAAGATAAGA AGCGGGAGAA CTTTGAACGG 
GGCAACCTGG AGCTGGAGAA GCGGAGACAA GCGCTCTTGG AGCAGCAGCG TAAAGAGCAG 
GAGCGCCTGG CCCAGCTGGA GCGGGCAGAA CAGGAGAGGA AGGAGCGGGA GCGCCAGGAG 
CAGGAGCGCA AAAGACAACT GGAGCTGGAA AAGCAGCTAG AGAAGCAGCG GGAGCTGGAG 
CGGCAGAGGG AGGAGGAGCG AAGGAAGGAA ATCGAGAGGC GGGAGGCTGC GAAACGGGAG 
CTTGAAAGGC AAAGACAGCT TGAATGGGAA CGGAATCGAA GACAAGAACT ATTAAATCAA 
AGAAACAAAG AACAAGAAGA CATAGTTGTA CTGAAAGCAA AGAAAAAGAC TTTGGAATTT 
GAATTAGAAG CTCTAAATGA TAAAAAACAT CAATTAGAAG GAAAACTTCA GGATATCAGA 
TGTCGACTGA CAACCCAAAG GCAAGAAATT GAGAGCACAA ACAAATCTAG AGAGTTGAGA 
ATTGCAGAAA TCACCCACCT ACAACAGCAA TTACAGGAGT CCCAGCAAAT GCTTGGAAGA 
CTTATTCCAG AAAAACAGAT ACTCAATGAC CAATTGAAAC AAGTTCAGCA AAACAGTTTG 
CACAGGGATT CACTTCTCAC ACTCAAAAGA GCCTTAGAAG CGAAAGAACT GGCTCGGCAG 
CAGCTACGAG ACCAACTGGA TGAAGTGGAG AAAGAAACTC GGTCAAAATT ACAGGAGATT 
GATATTTTCA ACAATCAGCT GAAGGAACTT AGAGAAATAC ATAATAAGCA GCAACTCCAG 
AAACAGAAGT CCATAGAGGC TGAACGACTG AAACAGAAGG AACAAGAACG AAAGATCCTG 
GAATTAGAAA AACAAAAAGA AGAAGCCCAA AGACGAGCTC AGGAAAGGGA CCAGCAGTGG 
CTGGAGCGCG TGCATCAGGA GGATGAGCAG CAGCGGCCAC GAAAACCCCA CGAGGAGGAG 
AAGCTCAAAA GGGAAGAGAG CGTCAAAAAG AAGGACAGTG AGGAGAAGGG CAAACCGGAA 
ATGCAAGACA AGCTGAGTCG GCTTTTCCAC CAGCATCAAG AACCAGCTAA GCCCGCTGTC 
CAGGCTCCCT GGTCCACTGC AGAAAAAGGC CCACTTACGA TTTCTGCACA GGAGAATGTA 
AAAGTGGTGT ATTACCGAGC ACTTTATCCC TTTGAGTCCA GGAGTCACGA TGAAATCACG 
ATCCAGCCGG GAGATATAGT CATGGTTAAA GGGGAATGGG TGGACGAAAG CCAAACTGGA 
GAACCGGGAT GGCTTGGAGG AGAATTAAAA GGAAAGACGG GATGGTTCCC TGCAAACTAC 
GCAGAGAAAA TCCCAGAAAA CGAGGTTCCC GCCCCAGTCA AACCAGTGAC TGAGGCGGCC 
TCCACCCCTA CACCCAAAGT GGCTGTGCGT GAGACCCCCG CCCCTTCATC TGCAACCCCT 
GCAGAGCCCT CCGAGACCCC CAACAACTGG GCCGACTTCA GCTCCACGTG GCCCACCAGC 
ACGAATGAGA AACCAGAAAC CGACAGCTGG GACGCATGGG CAACCCAGCC CTCCCTCACC 
GTGCCAAGCG CCGGGCAGCT GAGGCAGAGG TCGGCCTTCA CTCCAGCCAC GGCCACGGGC 
TCATCTCCGT CTCCTGTTCT AGGCCAGGGT GAAAAGGTGG AGGGGCTACA GGCGCAAGCC 
CTGTATCCGT GGAGAGCCAA GAAAGACAAC CATTTAAATT TTAACAAAAA CGATGTCATC 
ACCGTACTGG AGCAGCAAGA CATGTGGTGG TTTGGAGAAG TTCAAGGCCA GAAGGGTTGG 
TTCCCCAAGT CCTACGTGAA ACTCATTTCA GGGCCCATAA GGAAATCGAC AAGCATGGAT 
TCTGGTTCCT CAGAAAGTCC TGCAAGTCTG AAGAGGGTAG CTTCCCCAGC AGCCAAGCCA 
GCTGTGTCGG GAGAAGAAAT CGCACAGGTC ATTGCCTCCT ACACAGCTAC AGGTCCTGAA 
CAGCTTACCC TGGCCCCCGG TCAGCTGATC CTGATCCGGA AAAAGAACCC AGGTGGATGG 
TGGGAAGGAG AGCTACAGGC ACGTGGGAAA AAGCGCCAGA TAGGCTGGTT TCCTGCTAAT 
TACGTCAAAC TTTTAAGCCC TGGGACGAGC AAAATCACTC CAACAGACCC ACCAAAGCCA 
ACAGCTTTCC CAACAGTGTG CCAGGTGATC GGGATGTACG ACTACACCGC GCAGAACGAC 
GACGAGCTGG CCTTCAGCAA GGGCCAGGTC ATCAATGTCC TCAACAAGGA GGACCCCGAC 
TGGTGGAAGG GTGAAGTCCA CGGGCAGGTG GGGCTCTTCC CGTCCAACTA CGTGAAGCTG 
ACCACGGACA CGGACCCCAG CCAGCAATGG TGTTCAGACT TACACCTCTT GGATATGCTG 
ACCCCAACTG AAAGAAAGCG CCAGGGGTAC ATCCACGAGC TCATCGTCAC AGAGGAGAAC 
TATGTGAATG ACCTGCAGCT GGTCACAGAG ATCTTTCAGA AACCCCTGAT GGAGTCTGAG 
CTGCTGACAG AAAAAGAGGG TGCTATGATT TTTGTTAACT GGAAGGAGCT GATTATGTGT 
AATATCAAAC TACTGAAAGC GCTGAGGGTC CGCAAGAAGA TGTCTGGGGA GAAGATGCCC 
GTGAAGATGA TCGGAGACAT CCTGACGGCC CAGCTGCCAC ACATGCAGCC CTACATCCGC 
TTCTGCAGCT GCCAGCTCAA CGGGGCCGCC TTGATCCAGC AGAAGACTGA CGAGGCCCCA 
GACTTCAAGG AGTTTGTTAA AAGGCTGGCC ATGGACCCAC GCTGTAAGGG AATGCCCCTG 
TCTAGTTTTA TACTGAAGCC TATGCAACGA GTAACGAGAT ACCCACTGAT CATTAAAAAT 
ATCCTGGAAA ACACTCCCGA AAACCACCCA GACCACAGCC ACCTGAAGCA CGCCCTGGAG 
AAGGCAGAGG AGCTGTGCTC CCAGGTGAAC GAAGGGGTGC GGGAGAAGGA GAACTCGGAC 
CGACTGGAGT GGATCCAGGC CCACGTACTG TGTGAAGGCC TGTCAGAGCA ACTTGTGTTC 
AATTCCGTGA CCAACTGCCT GGGGCCGCGC AAGTTCCTGC ACAGTGGGAA GCTCTACAAG 
GCCAAGAGCA ACAAGGAGCT GTATGGCTTC CTCTTCAATG ACTTCCTGCT GCTGACCCAA 
ATCATCAAGC CCCTGGGCTC CTCAGGCACC GACAAAGTCT TCAGCCCCAA ATCAAACCTG 
CAGTATAAAA TGTACAAAAC ACCTATCTTC CTAAATGAGG TTCTAGTGAA GTTGCCCACT 
GACCCTTCTG GGGATGAACC CATCTTCCAC ATTTCCCACA TCGACCGAGT CTACACCCTC 
CGAGCAGAGA GCATAAACGA AAGGACTGCT TGGGTGCAGA AAATCAAAGC AGCTTCTGAA 
CTCTACATAG AGACAGAGAA GAAGAAGCGG GAGAAGGCGT ATCTGGTCCG CTCCCAAAGA 
GCCACAGGCA TCGGAAGGTT GATGGTGAAC GTGGTTGAAG GCATTGAGCT GAAACCCTGT 
CGCTCCCATG GGAAGAGTAA CCCATACTGC GAGGTGACCA TGGGTTCCCA GTGTCACATC 
ACCAAGACGA TCCAGGACAC TCTGAATCCC AAGTGGAATT CCAACTGCCA GTTCTTCATC 
AGAGACTTGG AGCAAGAGGT GCTGTGCATC ACCGTGTTTG AGAGGGACCA GTTCTCGCCA 
GATGATTTTT TGGGTCGGAC GGAGATCCGC GTGGCCGACA TCAAGAAGGA CCAGGGCTCC 
AAGGGTCCAG TTACGAAGTG TCTCCTGCTG CACGAAGTTC CCACGGGAGA GATCGTGGTC 
CGCTTGGACC TGCAGTTGTT CGATGAGCCG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITSN1 ( XM_025281353.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025137138.1 Bubalus bubalis intersectin 1 (ITSN1), transcript variant X10, mRNA.

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CloneID	OBu42479
Clone ID Related Accession (Same CDS sequence)	XM_025281353.1
Accession Version	XM_025281353.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4938bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	intersectin-1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037545.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCAGT TTCCCACACC TTTTGGTGGC AGCCTGGATA TTTGGGCCAT AACTGTAGAG 
GAAAGAGCAA AGCATGATCA ACAGTTCCAT AGTTTAAAGC CAATATCTGG ATTTATTACT 
GGTGATCAAG CTAGAAACTT TTTTTTTCAA TCTGGGTTAC CTCAGCCTGT TTTAGCACAG 
ATATGGGCGC TCGCTGATAT GAATAATGAT GGAAGGATGG ATCAAGTGGA GTTTTCCATT 
GCTATGAAAC TCATCAAGCT GAAGCTCCAA GGATATCAGC TCCCCTCTGC ACTTCCCCCT 
GTCATGAAAC AGCAACCAGT TGCTATTTCT AGTGCACCAG CGTTTGGTAT GGGAGGTATT 
GCCAGCATGC CGCCGCTGAC CGCTGTTGCT CCTGTGCCAA TGGGCTCCAT TCCCGTTGTT 
GGCATGTCTC CCCCCCTAGT ATCATCTGTT CCTGCAGCAG GGGTGCCCCC CCTGGCCAAC 
GGGGCGCCCC CGGTCATACA GCCTCTGCCG GCATTTGCTC ATCCTGCTGC CACATTGCCA 
AAGAGTTCTT CCTTTAGTAG ATCTGGCCCA GGGTCGCAGT TAAACACAAA ATTACAGAAG 
GCACAATCAT TTGATGTGGC CAGCGTCCCT CCTACAGCTG AGTGGGCTGT TCCTCAGTCA 
TCCAGGCTGA AATACAGACA GCTCTTCAAT AGTCATGACA AAACCATGAG TGGACACCTA 
ACAGGTCCCC AAGCAAGAAC TATTCTTATG CAATCAAGTT TACCACAGGC TCAGCTGGCT 
TCAATATGGA ATCTTTCTGA CATTGATCAA GATGGAAAAC TCACAGCAGA AGAATTTATC 
CTGGCTATGC ACCTCATTGA TGTAGCTATG TCTGGTCAAC CATTGCCACC GGTCCTGCCC 
CCAGAATACA TTCCACCTTC CTTTAGAAGA GTTCGATCTG GTAGTGGTAT ATCTGTCATA 
ACCTCAACTT CGGTGGATCA GAGGTTGCCA GAGGAACCAG TTTTGGAAGA TGAACAGCAA 
CAACTAGAAA AGAAACTACC TGTAACCTTT GAAGATAAGA AGCGGGAGAA CTTTGAACGG 
GGCAACCTGG AGCTGGAGAA GCGGAGACAA GCGCTCTTGG AGCAGCAGCG TAAAGAGCAG 
GAGCGCCTGG CCCAGCTGGA GCGGGCAGAA CAGGAGAGGA AGGAGCGGGA GCGCCAGGAG 
CAGGAGCGCA AAAGACAACT GGAGCTGGAA AAGCAGCTAG AGAAGCAGCG GGAGCTGGAG 
CGGCAGAGGG AGGAGGAGCG AAGGAAGGAA ATCGAGAGGC GGGAGGCTGC GAAACGGGAG 
CTTGAAAGGC AAAGACAGCT TGAATGGGAA CGGAATCGAA GACAAGAACT ATTAAATCAA 
AGAAACAAAG AACAAGAAGA CATAGTTGTA CTGAAAGCAA AGAAAAAGAC TTTGGAATTT 
GAATTAGAAG CTCTAAATGA TAAAAAACAT CAATTAGAAG GAAAACTTCA GGATATCAGA 
TGTCGACTGA CAACCCAAAG GCAAGAAATT GAGAGCACAA ACAAATCTAG AGAGTTGAGA 
ATTGCAGAAA TCACCCACCT ACAACAGCAA TTACAGGAGT CCCAGCAAAT GCTTGGAAGA 
CTTATTCCAG AAAAACAGAT ACTCAATGAC CAATTGAAAC AAGTTCAGCA AAACAGTTTG 
CACAGGGATT CACTTCTCAC ACTCAAAAGA GCCTTAGAAG CGAAAGAACT GGCTCGGCAG 
CAGCTACGAG ACCAACTGGA TGAAGTGGAG AAAGAAACTC GGTCAAAATT ACAGGAGATT 
GATATTTTCA ACAATCAGCT GAAGGAACTT AGAGAAATAC ATAATAAGCA GCAACTCCAG 
AAACAGAAGT CCATAGAGGC TGAACGACTG AAACAGAAGG AACAAGAACG AAAGATCCTG 
GAATTAGAAA AACAAAAAGA AGAAGCCCAA AGACGAGCTC AGGAAAGGGA CCAGCAGTGG 
CTGGAGCGCG TGCATCAGGA GGATGAGCAG CAGCGGCCAC GAAAACCCCA CGAGGAGGAG 
AAGCTCAAAA GGGAAGAGAG CGTCAAAAAG AAGGACAGTG AGGAGAAGGG CAAACCGGAA 
ATGCAAGACA AGCTGAGTCG GCTTTTCCAC CAGCATCAAG AACCAGCTAA GCCCGCTGTC 
CAGGCTCCCT GGTCCACTGC AGAAAAAGGC CCACTTACGA TTTCTGCACA GGAGAATGTA 
AAAGTGGTGT ATTACCGAGC ACTTTATCCC TTTGAGTCCA GGAGTCACGA TGAAATCACG 
ATCCAGCCGG GAGATATAGT CATGGTGGAC GAAAGCCAAA CTGGAGAACC GGGATGGCTT 
GGAGGAGAAT TAAAAGGAAA GACGGGATGG TTCCCTGCAA ACTACGCAGA GAAAATCCCA 
GAAAACGAGG TTCCCGCCCC AGTCAAACCA GTGACTGAGG CGGCCTCCAC CCCTACACCC 
AAAGTGGCTG TGCGTGAGAC CCCCGCCCCT TCATCTGCAA CCCCTGCAGA GCCCTCCGAG 
ACCCCCAACA ACTGGGCCGA CTTCAGCTCC ACGTGGCCCA CCAGCACGAA TGAGAAACCA 
GAAACCGACA GCTGGGACGC ATGGGCAACC CAGCCCTCCC TCACCGTGCC AAGCGCCGGG 
CAGCTGAGGC AGAGGTCGGC CTTCACTCCA GCCACGGCCA CGGGCTCATC TCCGTCTCCT 
GTTCTAGGCC AGGGTGAAAA GGTGGAGGGG CTACAGGCGC AAGCCCTGTA TCCGTGGAGA 
GCCAAGAAAG ACAACCATTT AAATTTTAAC AAAAACGATG TCATCACCGT ACTGGAGCAG 
CAAGACATGT GGTGGTTTGG AGAAGTTCAA GGCCAGAAGG GTTGGTTCCC CAAGTCCTAC 
GTGAAACTCA TTTCAGGGCC CATAAGGAAA TCGACAAGCA TGGATTCTGG TTCCTCAGAA 
AGTCCTGCAA GTCTGAAGAG GGTAGCTTCC CCAGCAGCCA AGCCAGCTGT GTCGGGAGAA 
GAAATCGCAC AGGTCATTGC CTCCTACACA GCTACAGGTC CTGAACAGCT TACCCTGGCC 
CCCGGTCAGC TGATCCTGAT CCGGAAAAAG AACCCAGGTG GATGGTGGGA AGGAGAGCTA 
CAGGCACGTG GGAAAAAGCG CCAGATAGGC TGGTTTCCTG CTAATTACGT CAAACTTTTA 
AGCCCTGGGA CGAGCAAAAT CACTCCAACA GACCCACCAA AGCCAACAGC TTTCCCAACA 
GTGTGCCAGG TGATCGGGAT GTACGACTAC ACCGCGCAGA ACGACGACGA GCTGGCCTTC 
AGCAAGGGCC AGGTCATCAA TGTCCTCAAC AAGGAGGACC CCGACTGGTG GAAGGGTGAA 
GTCCACGGGC AGGTGGGGCT CTTCCCGTCC AACTACGTGA AGCTGACCAC GGACACGGAC 
CCCAGCCAGC AATGGTGTTC AGACTTACAC CTCTTGGATA TGCTGACCCC AACTGAAAGA 
AAGCGCCAGG GGTACATCCA CGAGCTCATC GTCACAGAGG AGAACTATGT GAATGACCTG 
CAGCTGGTCA CAGAGATCTT TCAGAAACCC CTGATGGAGT CTGAGCTGCT GACAGAAAAA 
GAGGGTGCTA TGATTTTTGT TAACTGGAAG GAGCTGATTA TGTGTAATAT CAAACTACTG 
AAAGCGCTGA GGGTCCGCAA GAAGATGTCT GGGGAGAAGA TGCCCGTGAA GATGATCGGA 
GACATCCTGA CGGCCCAGCT GCCACACATG CAGCCCTACA TCCGCTTCTG CAGCTGCCAG 
CTCAACGGGG CCGCCTTGAT CCAGCAGAAG ACTGACGAGG CCCCAGACTT CAAGGAGTTT 
GTTAAAAGGC TGGCCATGGA CCCACGCTGT AAGGGAATGC CCCTGTCTAG TTTTATACTG 
AAGCCTATGC AACGAGTAAC GAGATACCCA CTGATCATTA AAAATATCCT GGAAAACACT 
CCCGAAAACC ACCCAGACCA CAGCCACCTG AAGCACGCCC TGGAGAAGGC AGAGGAGCTG 
TGCTCCCAGG TGAACGAAGG GGTGCGGGAG AAGGAGAACT CGGACCGACT GGAGTGGATC 
CAGGCCCACG TACTGTGTGA AGGCCTGTCA GAGCAACTTG TGTTCAATTC CGTGACCAAC 
TGCCTGGGGC CGCGCAAGTT CCTGCACAGT GGGAAGCTCT ACAAGGCCAA GAGCAACAAG 
GAGCTGTATG GCTTCCTCTT CAATGACTTC CTGCTGCTGA CCCAAATCAT CAAGCCCCTG 
GGCTCCTCAG GCACCGACAA AGTCTTCAGC CCCAAATCAA ACCTGCAGTA TAAAATGTAC 
AAAACACCTA TCTTCCTAAA TGAGGTTCTA GTGAAGTTGC CCACTGACCC TTCTGGGGAT 
GAACCCATCT TCCACATTTC CCACATCGAC CGAGTCTACA CCCTCCGAGC AGAGAGCATA 
AACGAAAGGA CTGCTTGGGT GCAGAAAATC AAAGCAGCTT CTGAACTCTA CATAGAGACA 
GAGAAGAAGA AGCGGGAGAA GGCGTATCTG GTCCGCTCCC AAAGAGCCAC AGGCATCGGA 
AGGTTGATGG TGAACGTGGT TGAAGGCATT GAGCTGAAAC CCTGTCGCTC CCATGGGAAG 
AGTAACCCAT ACTGCGAGGT GACCATGGGT TCCCAGTGTC ACATCACCAA GACGATCCAG 
GACACTCTGA ATCCCAAGTG GAATTCCAAC TGCCAGTTCT TCATCAGAGA CTTGGAGCAA 
GAGGTGCTGT GCATCACCGT GTTTGAGAGG GACCAGTTCT CGCCAGATGA TTTTTTGGGT 
CGGACGGAGA TCCGCGTGGC CGACATCAAG AAGGACCAGG GCTCCAAGGG TCCAGTTACG 
AAGTGTCTCC TGCTGCACGA AGTTCCCACG GGAGAGATCG TGGTCCGCTT GGACCTGCAG 
TTGTTCGATG AGCCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025137138.1
CDS	301..5238
Translation	MAQFPTPFGGSLDIWAITVEERAKHDQQFHSLKPISGFITGDQARNFFFQSGLPQPVLAQIWALADMNNDGRMDQVEFSIAMKLIKLKLQGYQLPSALPPVMKQQPVAISSAPAFGMGGIASMPPLTAVAPVPMGSIPVVGMSPPLVSSVPAAGVPPLANGAPPVIQPLPAFAHPAATLPKSSSFSRSGPGSQLNTKLQKAQSFDVASVPPTAEWAVPQSSRLKYRQLFNSHDKTMSGHLTGPQARTILMQSSLPQAQLASIWNLSDIDQDGKLTAEEFILAMHLIDVAMSGQPLPPVLPPEYIPPSFRRVRSGSGISVITSTSVDQRLPEEPVLEDEQQQLEKKLPVTFEDKKRENFERGNLELEKRRQALLEQQRKEQERLAQLERAEQERKERERQEQERKRQLELEKQLEKQRELERQREEERRKEIERREAAKRELERQRQLEWERNRRQELLNQRNKEQEDIVVLKAKKKTLEFELEALNDKKHQLEGKLQDIRCRLTTQRQEIESTNKSRELRIAEITHLQQQLQESQQMLGRLIPEKQILNDQLKQVQQNSLHRDSLLTLKRALEAKELARQQLRDQLDEVEKETRSKLQEIDIFNNQLKELREIHNKQQLQKQKSIEAERLKQKEQERKILELEKQKEEAQRRAQERDQQWLERVHQEDEQQRPRKPHEEEKLKREESVKKKDSEEKGKPEMQDKLSRLFHQHQEPAKPAVQAPWSTAEKGPLTISAQENVKVVYYRALYPFESRSHDEITIQPGDIVMVDESQTGEPGWLGGELKGKTGWFPANYAEKIPENEVPAPVKPVTEAASTPTPKVAVRETPAPSSATPAEPSETPNNWADFSSTWPTSTNEKPETDSWDAWATQPSLTVPSAGQLRQRSAFTPATATGSSPSPVLGQGEKVEGLQAQALYPWRAKKDNHLNFNKNDVITVLEQQDMWWFGEVQGQKGWFPKSYVKLISGPIRKSTSMDSGSSESPASLKRVASPAAKPAVSGEEIAQVIASYTATGPEQLTLAPGQLILIRKKNPGGWWEGELQARGKKRQIGWFPANYVKLLSPGTSKITPTDPPKPTAFPTVCQVIGMYDYTAQNDDELAFSKGQVINVLNKEDPDWWKGEVHGQVGLFPSNYVKLTTDTDPSQQWCSDLHLLDMLTPTERKRQGYIHELIVTEENYVNDLQLVTEIFQKPLMESELLTEKEGAMIFVNWKELIMCNIKLLKALRVRKKMSGEKMPVKMIGDILTAQLPHMQPYIRFCSCQLNGAALIQQKTDEAPDFKEFVKRLAMDPRCKGMPLSSFILKPMQRVTRYPLIIKNILENTPENHPDHSHLKHALEKAEELCSQVNEGVREKENSDRLEWIQAHVLCEGLSEQLVFNSVTNCLGPRKFLHSGKLYKAKSNKELYGFLFNDFLLLTQIIKPLGSSGTDKVFSPKSNLQYKMYKTPIFLNEVLVKLPTDPSGDEPIFHISHIDRVYTLRAESINERTAWVQKIKAASELYIETEKKKREKAYLVRSQRATGIGRLMVNVVEGIELKPCRSHGKSNPYCEVTMGSQCHITKTIQDTLNPKWNSNCQFFIRDLEQEVLCITVFERDQFSPDDFLGRTEIRVADIKKDQGSKGPVTKCLLLHEVPTGEIVVRLDLQLFDEP

Target ORF information:

RefSeq Version	XM_025281353.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis intersectin 1 (ITSN1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025281353.1

ORF Insert Sequence:

ATGGCTCAGT TTCCCACACC TTTTGGTGGC AGCCTGGATA TTTGGGCCAT AACTGTAGAG 
GAAAGAGCAA AGCATGATCA ACAGTTCCAT AGTTTAAAGC CAATATCTGG ATTTATTACT 
GGTGATCAAG CTAGAAACTT TTTTTTTCAA TCTGGGTTAC CTCAGCCTGT TTTAGCACAG 
ATATGGGCGC TCGCTGATAT GAATAATGAT GGAAGGATGG ATCAAGTGGA GTTTTCCATT 
GCTATGAAAC TCATCAAGCT GAAGCTCCAA GGATATCAGC TCCCCTCTGC ACTTCCCCCT 
GTCATGAAAC AGCAACCAGT TGCTATTTCT AGTGCACCAG CGTTTGGTAT GGGAGGTATT 
GCCAGCATGC CGCCGCTGAC CGCTGTTGCT CCTGTGCCAA TGGGCTCCAT TCCCGTTGTT 
GGCATGTCTC CCCCCCTAGT ATCATCTGTT CCTGCAGCAG GGGTGCCCCC CCTGGCCAAC 
GGGGCGCCCC CGGTCATACA GCCTCTGCCG GCATTTGCTC ATCCTGCTGC CACATTGCCA 
AAGAGTTCTT CCTTTAGTAG ATCTGGCCCA GGGTCGCAGT TAAACACAAA ATTACAGAAG 
GCACAATCAT TTGATGTGGC CAGCGTCCCT CCTACAGCTG AGTGGGCTGT TCCTCAGTCA 
TCCAGGCTGA AATACAGACA GCTCTTCAAT AGTCATGACA AAACCATGAG TGGACACCTA 
ACAGGTCCCC AAGCAAGAAC TATTCTTATG CAATCAAGTT TACCACAGGC TCAGCTGGCT 
TCAATATGGA ATCTTTCTGA CATTGATCAA GATGGAAAAC TCACAGCAGA AGAATTTATC 
CTGGCTATGC ACCTCATTGA TGTAGCTATG TCTGGTCAAC CATTGCCACC GGTCCTGCCC 
CCAGAATACA TTCCACCTTC CTTTAGAAGA GTTCGATCTG GTAGTGGTAT ATCTGTCATA 
ACCTCAACTT CGGTGGATCA GAGGTTGCCA GAGGAACCAG TTTTGGAAGA TGAACAGCAA 
CAACTAGAAA AGAAACTACC TGTAACCTTT GAAGATAAGA AGCGGGAGAA CTTTGAACGG 
GGCAACCTGG AGCTGGAGAA GCGGAGACAA GCGCTCTTGG AGCAGCAGCG TAAAGAGCAG 
GAGCGCCTGG CCCAGCTGGA GCGGGCAGAA CAGGAGAGGA AGGAGCGGGA GCGCCAGGAG 
CAGGAGCGCA AAAGACAACT GGAGCTGGAA AAGCAGCTAG AGAAGCAGCG GGAGCTGGAG 
CGGCAGAGGG AGGAGGAGCG AAGGAAGGAA ATCGAGAGGC GGGAGGCTGC GAAACGGGAG 
CTTGAAAGGC AAAGACAGCT TGAATGGGAA CGGAATCGAA GACAAGAACT ATTAAATCAA 
AGAAACAAAG AACAAGAAGA CATAGTTGTA CTGAAAGCAA AGAAAAAGAC TTTGGAATTT 
GAATTAGAAG CTCTAAATGA TAAAAAACAT CAATTAGAAG GAAAACTTCA GGATATCAGA 
TGTCGACTGA CAACCCAAAG GCAAGAAATT GAGAGCACAA ACAAATCTAG AGAGTTGAGA 
ATTGCAGAAA TCACCCACCT ACAACAGCAA TTACAGGAGT CCCAGCAAAT GCTTGGAAGA 
CTTATTCCAG AAAAACAGAT ACTCAATGAC CAATTGAAAC AAGTTCAGCA AAACAGTTTG 
CACAGGGATT CACTTCTCAC ACTCAAAAGA GCCTTAGAAG CGAAAGAACT GGCTCGGCAG 
CAGCTACGAG ACCAACTGGA TGAAGTGGAG AAAGAAACTC GGTCAAAATT ACAGGAGATT 
GATATTTTCA ACAATCAGCT GAAGGAACTT AGAGAAATAC ATAATAAGCA GCAACTCCAG 
AAACAGAAGT CCATAGAGGC TGAACGACTG AAACAGAAGG AACAAGAACG AAAGATCCTG 
GAATTAGAAA AACAAAAAGA AGAAGCCCAA AGACGAGCTC AGGAAAGGGA CCAGCAGTGG 
CTGGAGCGCG TGCATCAGGA GGATGAGCAG CAGCGGCCAC GAAAACCCCA CGAGGAGGAG 
AAGCTCAAAA GGGAAGAGAG CGTCAAAAAG AAGGACAGTG AGGAGAAGGG CAAACCGGAA 
ATGCAAGACA AGCTGAGTCG GCTTTTCCAC CAGCATCAAG AACCAGCTAA GCCCGCTGTC 
CAGGCTCCCT GGTCCACTGC AGAAAAAGGC CCACTTACGA TTTCTGCACA GGAGAATGTA 
AAAGTGGTGT ATTACCGAGC ACTTTATCCC TTTGAGTCCA GGAGTCACGA TGAAATCACG 
ATCCAGCCGG GAGATATAGT CATGGTGGAC GAAAGCCAAA CTGGAGAACC GGGATGGCTT 
GGAGGAGAAT TAAAAGGAAA GACGGGATGG TTCCCTGCAA ACTACGCAGA GAAAATCCCA 
GAAAACGAGG TTCCCGCCCC AGTCAAACCA GTGACTGAGG CGGCCTCCAC CCCTACACCC 
AAAGTGGCTG TGCGTGAGAC CCCCGCCCCT TCATCTGCAA CCCCTGCAGA GCCCTCCGAG 
ACCCCCAACA ACTGGGCCGA CTTCAGCTCC ACGTGGCCCA CCAGCACGAA TGAGAAACCA 
GAAACCGACA GCTGGGACGC ATGGGCAACC CAGCCCTCCC TCACCGTGCC AAGCGCCGGG 
CAGCTGAGGC AGAGGTCGGC CTTCACTCCA GCCACGGCCA CGGGCTCATC TCCGTCTCCT 
GTTCTAGGCC AGGGTGAAAA GGTGGAGGGG CTACAGGCGC AAGCCCTGTA TCCGTGGAGA 
GCCAAGAAAG ACAACCATTT AAATTTTAAC AAAAACGATG TCATCACCGT ACTGGAGCAG 
CAAGACATGT GGTGGTTTGG AGAAGTTCAA GGCCAGAAGG GTTGGTTCCC CAAGTCCTAC 
GTGAAACTCA TTTCAGGGCC CATAAGGAAA TCGACAAGCA TGGATTCTGG TTCCTCAGAA 
AGTCCTGCAA GTCTGAAGAG GGTAGCTTCC CCAGCAGCCA AGCCAGCTGT GTCGGGAGAA 
GAAATCGCAC AGGTCATTGC CTCCTACACA GCTACAGGTC CTGAACAGCT TACCCTGGCC 
CCCGGTCAGC TGATCCTGAT CCGGAAAAAG AACCCAGGTG GATGGTGGGA AGGAGAGCTA 
CAGGCACGTG GGAAAAAGCG CCAGATAGGC TGGTTTCCTG CTAATTACGT CAAACTTTTA 
AGCCCTGGGA CGAGCAAAAT CACTCCAACA GACCCACCAA AGCCAACAGC TTTCCCAACA 
GTGTGCCAGG TGATCGGGAT GTACGACTAC ACCGCGCAGA ACGACGACGA GCTGGCCTTC 
AGCAAGGGCC AGGTCATCAA TGTCCTCAAC AAGGAGGACC CCGACTGGTG GAAGGGTGAA 
GTCCACGGGC AGGTGGGGCT CTTCCCGTCC AACTACGTGA AGCTGACCAC GGACACGGAC 
CCCAGCCAGC AATGGTGTTC AGACTTACAC CTCTTGGATA TGCTGACCCC AACTGAAAGA 
AAGCGCCAGG GGTACATCCA CGAGCTCATC GTCACAGAGG AGAACTATGT GAATGACCTG 
CAGCTGGTCA CAGAGATCTT TCAGAAACCC CTGATGGAGT CTGAGCTGCT GACAGAAAAA 
GAGGGTGCTA TGATTTTTGT TAACTGGAAG GAGCTGATTA TGTGTAATAT CAAACTACTG 
AAAGCGCTGA GGGTCCGCAA GAAGATGTCT GGGGAGAAGA TGCCCGTGAA GATGATCGGA 
GACATCCTGA CGGCCCAGCT GCCACACATG CAGCCCTACA TCCGCTTCTG CAGCTGCCAG 
CTCAACGGGG CCGCCTTGAT CCAGCAGAAG ACTGACGAGG CCCCAGACTT CAAGGAGTTT 
GTTAAAAGGC TGGCCATGGA CCCACGCTGT AAGGGAATGC CCCTGTCTAG TTTTATACTG 
AAGCCTATGC AACGAGTAAC GAGATACCCA CTGATCATTA AAAATATCCT GGAAAACACT 
CCCGAAAACC ACCCAGACCA CAGCCACCTG AAGCACGCCC TGGAGAAGGC AGAGGAGCTG 
TGCTCCCAGG TGAACGAAGG GGTGCGGGAG AAGGAGAACT CGGACCGACT GGAGTGGATC 
CAGGCCCACG TACTGTGTGA AGGCCTGTCA GAGCAACTTG TGTTCAATTC CGTGACCAAC 
TGCCTGGGGC CGCGCAAGTT CCTGCACAGT GGGAAGCTCT ACAAGGCCAA GAGCAACAAG 
GAGCTGTATG GCTTCCTCTT CAATGACTTC CTGCTGCTGA CCCAAATCAT CAAGCCCCTG 
GGCTCCTCAG GCACCGACAA AGTCTTCAGC CCCAAATCAA ACCTGCAGTA TAAAATGTAC 
AAAACACCTA TCTTCCTAAA TGAGGTTCTA GTGAAGTTGC CCACTGACCC TTCTGGGGAT 
GAACCCATCT TCCACATTTC CCACATCGAC CGAGTCTACA CCCTCCGAGC AGAGAGCATA 
AACGAAAGGA CTGCTTGGGT GCAGAAAATC AAAGCAGCTT CTGAACTCTA CATAGAGACA 
GAGAAGAAGA AGCGGGAGAA GGCGTATCTG GTCCGCTCCC AAAGAGCCAC AGGCATCGGA 
AGGTTGATGG TGAACGTGGT TGAAGGCATT GAGCTGAAAC CCTGTCGCTC CCATGGGAAG 
AGTAACCCAT ACTGCGAGGT GACCATGGGT TCCCAGTGTC ACATCACCAA GACGATCCAG 
GACACTCTGA ATCCCAAGTG GAATTCCAAC TGCCAGTTCT TCATCAGAGA CTTGGAGCAA 
GAGGTGCTGT GCATCACCGT GTTTGAGAGG GACCAGTTCT CGCCAGATGA TTTTTTGGGT 
CGGACGGAGA TCCGCGTGGC CGACATCAAG AAGGACCAGG GCTCCAAGGG TCCAGTTACG 
AAGTGTCTCC TGCTGCACGA AGTTCCCACG GGAGAGATCG TGGTCCGCTT GGACCTGCAG 
TTGTTCGATG AGCCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	ITSN1
Entrez Gene ID	102389170
Full Name	intersectin 1
Gene Type	protein-coding
Organism	Bubalus bubalis(water buffalo)

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

ITSN1 cDNA ORF clone, Bubalus bubalis(water buffalo)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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