LOC103866408 cDNA ORF clone, Brassica rapa(field mustard)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC103866408
Entrez Gene ID	103866408
Full Name	pectinesterase 5-like
Gene Type	protein-coding
Organism	Brassica rapa(field mustard)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009144323.2	XP_009142571.1	pectinesterase 5-like

Pathways from BioSystems
Gene Ontology

The following LOC103866408 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC103866408 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBr150641	XM_009144323.2 Latest version!	Brassica rapa pectinesterase 5-like (LOC103866408), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC103866408 ( XM_009144323.2 ) cDNA ORF clone, Brassica rapa(field mustard) -> XP_009142571.1 Brassica rapa pectinesterase 5-like (LOC103866408), mRNA.

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CloneID	OBr150641
Clone ID Related Accession (Same CDS sequence)	XM_009144323.2
Accession Version	XM_009144323.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1779bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-10-12
Organism	Brassica rapa(field mustard)
Product	pectinesterase 5-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_024799.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Oct 13, 2016 this sequence version replaced XM_009144323.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Brassica rapa Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTGGAA AAGTTGTTGT CTCTGTGGCC TCCGTCCTTC TATTGGTCGG AGTAGCCATT 
GGAGTCGTTG CCATCGTTAA TAAAAGCGGC AACACTAATT TGTCTCCACA GATGAAAGCT 
GTTCAAGGCA TTTGCCAAGC CACTGCTGAC AAAGCCTCAT GTGTTAAAAC TCTCGAGCCC 
GTCAAGAGCG ATGACCCAAA CAAACTAATC AAGGCCTTCA TGCTCGCTAC ACAAGATGCA 
CTAACCAAAT CATCAAACTT CACGGATAAA ACCGAAGGCA ACATGGGTTC GAGCATCTCC 
CCAAACAACA AAGCCGTTCT TGATTACTGC AGGAAAGTTT TCATGTACGC GCTTGAGGAT 
CTCGGTACCA TTCTTGAGGA AATGGGTGAA GATCTTAACA CGATCGGTAA CAAATTCGAC 
CAGCTTAAGC AATGGTTAAC CGGTGTTTAC AATTACCAAA CCGATTGTCT TGACGATATT 
CAAGAAGATG ATTTGAGAAA GACTATTGCT GAAGGCATTT CTAGCTCCAA GATTCTTACT 
GGTAATGCCA TCGACATCTT CCACACAGTT GTTAGCGCCA TGGCCAAGAT TGACGCTAAA 
GTCGATGATT TCAAGAACAT GACAAGTGGA ATCTTCACTC CTTCAGCTCC GGCCAACAAA 
GAAGCCGCTC CTGTCGTTGA CACTCCCGTG GCCGACCCTG ACGGTCTTTC TCGTCGTCTT 
CTTGAAGACC TTGACGACCT TGGAGTCCCA ACATGGGTTT CAGGTGCAGA CAGGAAGCTC 
ATGGCTAACG CTGGACGTGG CCGAAGAGGA GGCGGTGGAG CTCCTAGAAT CAGAGCAACC 
TATGTGGTGG CTAAGGATGG AAGCGGACAG TTTAAGTCAA TTCAACAAGC TGTTAACGCT 
TGTCCCGACA AAAACGCTGG AAGATGCATC ATCCACATTA AGGCTGGTAT CTACAGAGAG 
CAAGTTATCA TCCCTAAGAA GAAGAACAAC ATCTTCATGT TCGGAGATGG TGCAAGAAAG 
ACCGTTATTA CTTACAACAG AAGTGTTGGT CTCAGTTCTG GAACCACCAC TTCTACTAGT 
GGCACAGTCC AGGTTGAATC TGAAGGATTC ATGGCTAAAT GGATCGGATT CAAGAACACA 
GCCGGTCCAA ATGGGCACCA AGCTGTTGCT ATCAGAGTCA ACGGAGACCG TGCCGTGATC 
TTCAACTGCA GATTCGACGG TTACCAAGAC ACACTATACG TCAACAACGG TCGTCAATTC 
TACAGAAACT GCGTCGTTTC AGGAACAGTC GACTTCATCT TCGGCAAATC CGCCACCGTT 
ATCCAAAACT CACTCATCGT CATCCGCAAA GGAAACAAGG GACAATACAA CACCGTCACA 
GCCGACGGTA ACGAAAAGGG TCTATCCATG AAAATCGGTA TCGTCATCCA AAACTGCCGC 
ATCGTTCCCG ACAAGAAGCT AGCGCCGGAG AGGCTGACCG TTGAGTCGTA CTTGGGAAGG 
CCGTGGAAGC AGTACTCCAC CACCGTGGTG ATCAACACCG AGATCGGAGA TGTGATTAGA 
CCAGAAGGTT GGAGACTGTG GGACGGAGAG AGCTTCCACA AGACATGTAG GTACGTTGAG 
TACAACAACC GTGGACCAGG AGCTAACACT AACAGGAGAG TTAACTGGGC TAAGGTCGCT 
AGGTCTGCTG GTGAGATTAA TCAGTTCACC GTGGCTAACT GGTTAGCTCC GGTTAACTGG 
ATCCAAGAAG CTAACGTGCC AGTCACGCTT GGATTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009142571.1
CDS	133..1911
Translation	MIGKVVVSVASVLLLVGVAIGVVAIVNKSGNTNLSPQMKAVQGICQATADKASCVKTLEPVKSDDPNKLIKAFMLATQDALTKSSNFTDKTEGNMGSSISPNNKAVLDYCRKVFMYALEDLGTILEEMGEDLNTIGNKFDQLKQWLTGVYNYQTDCLDDIQEDDLRKTIAEGISSSKILTGNAIDIFHTVVSAMAKIDAKVDDFKNMTSGIFTPSAPANKEAAPVVDTPVADPDGLSRRLLEDLDDLGVPTWVSGADRKLMANAGRGRRGGGGAPRIRATYVVAKDGSGQFKSIQQAVNACPDKNAGRCIIHIKAGIYREQVIIPKKKNNIFMFGDGARKTVITYNRSVGLSSGTTTSTSGTVQVESEGFMAKWIGFKNTAGPNGHQAVAIRVNGDRAVIFNCRFDGYQDTLYVNNGRQFYRNCVVSGTVDFIFGKSATVIQNSLIVIRKGNKGQYNTVTADGNEKGLSMKIGIVIQNCRIVPDKKLAPERLTVESYLGRPWKQYSTTVVINTEIGDVIRPEGWRLWDGESFHKTCRYVEYNNRGPGANTNRRVNWAKVARSAGEINQFTVANWLAPVNWIQEANVPVTLGL

Target ORF information:

RefSeq Version	XM_009144323.2
Organism	Brassica rapa(field mustard)
Definition	Brassica rapa pectinesterase 5-like (LOC103866408), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009144323.2

ORF Insert Sequence:

ATGATTGGAA AAGTTGTTGT CTCTGTGGCC TCCGTCCTTC TATTGGTCGG AGTAGCCATT 
GGAGTCGTTG CCATCGTTAA TAAAAGCGGC AACACTAATT TGTCTCCACA GATGAAAGCT 
GTTCAAGGCA TTTGCCAAGC CACTGCTGAC AAAGCCTCAT GTGTTAAAAC TCTCGAGCCC 
GTCAAGAGCG ATGACCCAAA CAAACTAATC AAGGCCTTCA TGCTCGCTAC ACAAGATGCA 
CTAACCAAAT CATCAAACTT CACGGATAAA ACCGAAGGCA ACATGGGTTC GAGCATCTCC 
CCAAACAACA AAGCCGTTCT TGATTACTGC AGGAAAGTTT TCATGTACGC GCTTGAGGAT 
CTCGGTACCA TTCTTGAGGA AATGGGTGAA GATCTTAACA CGATCGGTAA CAAATTCGAC 
CAGCTTAAGC AATGGTTAAC CGGTGTTTAC AATTACCAAA CCGATTGTCT TGACGATATT 
CAAGAAGATG ATTTGAGAAA GACTATTGCT GAAGGCATTT CTAGCTCCAA GATTCTTACT 
GGTAATGCCA TCGACATCTT CCACACAGTT GTTAGCGCCA TGGCCAAGAT TGACGCTAAA 
GTCGATGATT TCAAGAACAT GACAAGTGGA ATCTTCACTC CTTCAGCTCC GGCCAACAAA 
GAAGCCGCTC CTGTCGTTGA CACTCCCGTG GCCGACCCTG ACGGTCTTTC TCGTCGTCTT 
CTTGAAGACC TTGACGACCT TGGAGTCCCA ACATGGGTTT CAGGTGCAGA CAGGAAGCTC 
ATGGCTAACG CTGGACGTGG CCGAAGAGGA GGCGGTGGAG CTCCTAGAAT CAGAGCAACC 
TATGTGGTGG CTAAGGATGG AAGCGGACAG TTTAAGTCAA TTCAACAAGC TGTTAACGCT 
TGTCCCGACA AAAACGCTGG AAGATGCATC ATCCACATTA AGGCTGGTAT CTACAGAGAG 
CAAGTTATCA TCCCTAAGAA GAAGAACAAC ATCTTCATGT TCGGAGATGG TGCAAGAAAG 
ACCGTTATTA CTTACAACAG AAGTGTTGGT CTCAGTTCTG GAACCACCAC TTCTACTAGT 
GGCACAGTCC AGGTTGAATC TGAAGGATTC ATGGCTAAAT GGATCGGATT CAAGAACACA 
GCCGGTCCAA ATGGGCACCA AGCTGTTGCT ATCAGAGTCA ACGGAGACCG TGCCGTGATC 
TTCAACTGCA GATTCGACGG TTACCAAGAC ACACTATACG TCAACAACGG TCGTCAATTC 
TACAGAAACT GCGTCGTTTC AGGAACAGTC GACTTCATCT TCGGCAAATC CGCCACCGTT 
ATCCAAAACT CACTCATCGT CATCCGCAAA GGAAACAAGG GACAATACAA CACCGTCACA 
GCCGACGGTA ACGAAAAGGG TCTATCCATG AAAATCGGTA TCGTCATCCA AAACTGCCGC 
ATCGTTCCCG ACAAGAAGCT AGCGCCGGAG AGGCTGACCG TTGAGTCGTA CTTGGGAAGG 
CCGTGGAAGC AGTACTCCAC CACCGTGGTG ATCAACACCG AGATCGGAGA TGTGATTAGA 
CCAGAAGGTT GGAGACTGTG GGACGGAGAG AGCTTCCACA AGACATGTAG GTACGTTGAG 
TACAACAACC GTGGACCAGG AGCTAACACT AACAGGAGAG TTAACTGGGC TAAGGTCGCT 
AGGTCTGCTG GTGAGATTAA TCAGTTCACC GTGGCTAACT GGTTAGCTCC GGTTAACTGG 
ATCCAAGAAG CTAACGTGCC AGTCACGCTT GGATTATAA