LOC109488122 cDNA ORF clone, Branchiostoma belcheri(Belcher's lancelet)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC109488122
Entrez Gene ID	109488122
Full Name	TGF-beta-activated kinase 1 and MAP3K7-binding protein 3-like
Gene Type	protein-coding
Organism	Branchiostoma belcheri(Belcher's lancelet)

mRNA and Protein(s)

mRNA	Protein	Name
XM_019792286.1	XP_019647845.1	TGF-beta-activated kinase 1 and MAP3K7-binding protein 3-like isoform X2
XM_019792284.1	XP_019647843.1	TGF-beta-activated kinase 1 and MAP3K7-binding protein 3-like isoform X1
XM_019792285.1	XP_019647844.1	TGF-beta-activated kinase 1 and MAP3K7-binding protein 3-like isoform X1
XM_019792287.1	XP_019647846.1	TGF-beta-activated kinase 1 and MAP3K7-binding protein 3-like isoform X3

Pathways from BioSystems
Gene Ontology

The following LOC109488122 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC109488122 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBr198361	XM_019792284.1 Latest version!	Branchiostoma belcheri TGF-beta-activated kinase 1 and MAP3K7-binding protein 3-like (LOC109488122), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
OBr198361	XM_019792285.1 Latest version!	Branchiostoma belcheri TGF-beta-activated kinase 1 and MAP3K7-binding protein 3-like (LOC109488122), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
OBr198362	XM_019792286.1 Latest version!	Branchiostoma belcheri TGF-beta-activated kinase 1 and MAP3K7-binding protein 3-like (LOC109488122), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
OBr198363	XM_019792287.1 Latest version!	Branchiostoma belcheri TGF-beta-activated kinase 1 and MAP3K7-binding protein 3-like (LOC109488122), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC109488122 ( XM_019792284.1 ) cDNA ORF clone, Branchiostoma belcheri(Belcher's lancelet) -> XP_019647843.1 Branchiostoma belcheri TGF-beta-activated kinase 1 and MAP3K7-binding protein 3-like (LOC109488122), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBr198361
Clone ID Related Accession (Same CDS sequence)	XM_019792284.1 , XM_019792285.1
Accession Version	XM_019792284.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2358bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-12-27
Organism	Branchiostoma belcheri(Belcher's lancelet)
Product	TGF-beta-activated kinase 1 and MAP3K7-binding protein 3-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017804587.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Branchiostoma belcheri Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCAGG GCGTGGCCGG TAGCCATGGC AACATGCGCA TGTTCGAGGA GCTGTGCCGA 
CGATTCCAGG AGGTGCCGCA GAACGTGGTG GTGGACGTCA TGAGAAAGAA CGGGAGTAAC 
TTCGAGCTGT GCTGCCAGGA GCTGCAGCAG GAGAGTGGGA AGTATCTGTA CGGAGACTTC 
CAGACAGCGG CGGCGGGGGA AAACGACATG ATGAGCAACC ACATGAAACA GCTCAACATC 
GGCGGGAAAC AACCACCAGA CGACCACAGA CACGGGTTAC ACCATTGCGC GAGCACCCCC 
GCGTTTCTAC CTAGTGGCGC GAACCAGCAC GGGACTTACC ACGAGGATGT TCACACGGCG 
CCAGTTTTAC CGGTGGGGCA AAATCCGTTT TTAGCGTCGA TGAGATCGCC ACAGCACGTA 
CAGAGATACG GCCCAACGGT TCAGATGTAC GCGGCACAGT TCGGACAAGG AGGGAACCAT 
TATGTCGGTG GGACTATGCC GCCTGGATAT GGACAGTCGT CGGGGAGAAG TACGCCTAGC 
CCTGCTCCAA CACCCCCTCC CCCGGGAAAC CAGCAACAGA TCTCCCCCCA GACCCCTCAG 
ACCCCGCCCC AGGTTCACTC CCCGCCCCCC TCACAGATGC ACACCCCCTC CCCCCAGGAA 
CAGAGACAAT CCCGCAGCCA GACCAACTCC CCCTTCCCCC CCATGTACCA GCAGCTCACC 
TCCCCTCAGC AACAATACAT GTACTTCCCA CAGCTCCCCT ACCAGATGAT GTCCCCACCC 
CAGGGGTACC CTCCGCAGGG GTCCTTCCCC TCCCCCCAGG GGCAGTACGG CGGGGTCTTC 
ACCTCCCCCC AGGGGCAGCC CCTTCCGGTG GTGCCGGGGT TTTACCAGCC GTACTCGCAA 
GGACCTCAGC AAGGACAGTT CATGTCGGGT CAGTGGCCTC AGGTGACCCC GCCGGGGTAT 
AACCCCATGT ACGACCGCAG GATGTCCGGC GGGAGCGAGA GTTCAGGTCA GGGGTCACCT 
TCCCACGCTC CTCAGCTGCC GCCGTGGAAC TTTCAACAAC AGCAGCAGCA ACAGCAGGCG 
TGGCACGGAG GTCAAGGTCA AATGCACGGG GGTCAAGGTC AAATGCACGG AGGTCAAGGT 
CAAATGCACG GAGGTCAAGG TCAAATGTTT CCAAGGGCGC ACAGCCCGTA CCACAGAACG 
GACTCCCCGC GCGGCGGGCA CTTAGACGCC CACTGGTCGC CCAAACATTC GCCACGCAGC 
CAGAGCCCCA GCCCAGGGAG GTCAGGGTGG GGTCACGGGG CGACGCCGCC GCACCAGTAC 
ACCCCTCCCG GGCAGGGTGG GCAGCCCCGC CACCACGGCC CCTACCAGCG CTCGCTCTCC 
TCACAGAACC ACCAGTATTC CAGCCTCAAC CTCCCCCCGC ACGGCGGCTA CCACCCCCCA 
CAGCCGCCCA ACAAAACTCA CGACTCACCC CGAAGCTCCT TAGTGTTCAC CCAGCCCCTC 
CACGTCCCCA CACACTCCAC CTACTCCTCC CCAGGCCGAC CAGCCATGTC TGTCACAACA 
CCAACCTCCA ACAACTCCAC ACCCACGCCA ACAAAAACAC CCAGCCAGCC CAACCTGCTC 
ACACCCGAGC ACCCGGAGGA GCTAAGGTCG GAAACGGGCA CACCCCCGCC CCCGATTCTC 
CCCGTCATCT CCCCAGCATC CAGCCATAGC AGCCTGTCGT CAGCAGAGAG CAGAGACCGT 
GGACAGAGGC CCAGCACTAG TAATGACAAC TATGCATACG AACAAGCGTT GCTGCTTCAC 
CAGCGGGCGC GGATGGAGAA GTTGAAGAAG GACTGGAACG GGGAGAAGCA GAGACTCGCG 
CAGGTCAGAG TAGAGGTCGA CGAGATGGAA CGTGACCTTC AGGACCGCAG AAAACGCAAC 
GCGACCTCCA AATGTCCCAC GGTAGAAGAG ATAGTGCGTA AGCGGGAACA CAACAGGCAG 
CTGCAGATAG AGATAGACTG TATGACAAAG GAGACTGACC TGCTACAGCA GAGTAGATAC 
TACGTATCCG ACCCGGTGGA CGTGCCGAAC TTCTACGCTA ACCTGGGCGC CACGGGACAG 
CTGGGTCCGG TCCCCCCGCC TAGCGCCGCC TCACAGCATA GACGAAACCT GTCCAGCTCC 
ACGGCAGCTG AAGGTGGTGT GACAGAAAGG GAGGGGGAGG GGGGTTGGCA GATGGTGAAC 
TATTCAGCAG AAGCGCCCAA CCCCAGCGCA GCCCCAGAGG AGGCGGAGGG TCCGCAGTGG 
AACTGTCGAG CCTGTACCTT CCTCAACCAC CCACAGCTAG ACAAATGTGA AATCTGCGAG 
ATGCCCAGAG TTATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019647843.1
CDS	240..2597
Translation	MAQGVAGSHGNMRMFEELCRRFQEVPQNVVVDVMRKNGSNFELCCQELQQESGKYLYGDFQTAAAGENDMMSNHMKQLNIGGKQPPDDHRHGLHHCASTPAFLPSGANQHGTYHEDVHTAPVLPVGQNPFLASMRSPQHVQRYGPTVQMYAAQFGQGGNHYVGGTMPPGYGQSSGRSTPSPAPTPPPPGNQQQISPQTPQTPPQVHSPPPSQMHTPSPQEQRQSRSQTNSPFPPMYQQLTSPQQQYMYFPQLPYQMMSPPQGYPPQGSFPSPQGQYGGVFTSPQGQPLPVVPGFYQPYSQGPQQGQFMSGQWPQVTPPGYNPMYDRRMSGGSESSGQGSPSHAPQLPPWNFQQQQQQQQAWHGGQGQMHGGQGQMHGGQGQMHGGQGQMFPRAHSPYHRTDSPRGGHLDAHWSPKHSPRSQSPSPGRSGWGHGATPPHQYTPPGQGGQPRHHGPYQRSLSSQNHQYSSLNLPPHGGYHPPQPPNKTHDSPRSSLVFTQPLHVPTHSTYSSPGRPAMSVTTPTSNNSTPTPTKTPSQPNLLTPEHPEELRSETGTPPPPILPVISPASSHSSLSSAESRDRGQRPSTSNDNYAYEQALLLHQRARMEKLKKDWNGEKQRLAQVRVEVDEMERDLQDRRKRNATSKCPTVEEIVRKREHNRQLQIEIDCMTKETDLLQQSRYYVSDPVDVPNFYANLGATGQLGPVPPPSAASQHRRNLSSSTAAEGGVTEREGEGGWQMVNYSAEAPNPSAAPEEAEGPQWNCRACTFLNHPQLDKCEICEMPRVI

Target ORF information:

RefSeq Version	XM_019792284.1
Organism	Branchiostoma belcheri(Belcher's lancelet)
Definition	Branchiostoma belcheri TGF-beta-activated kinase 1 and MAP3K7-binding protein 3-like (LOC109488122), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019792284.1

ORF Insert Sequence:

ATGGCGCAGG GCGTGGCCGG TAGCCATGGC AACATGCGCA TGTTCGAGGA GCTGTGCCGA 
CGATTCCAGG AGGTGCCGCA GAACGTGGTG GTGGACGTCA TGAGAAAGAA CGGGAGTAAC 
TTCGAGCTGT GCTGCCAGGA GCTGCAGCAG GAGAGTGGGA AGTATCTGTA CGGAGACTTC 
CAGACAGCGG CGGCGGGGGA AAACGACATG ATGAGCAACC ACATGAAACA GCTCAACATC 
GGCGGGAAAC AACCACCAGA CGACCACAGA CACGGGTTAC ACCATTGCGC GAGCACCCCC 
GCGTTTCTAC CTAGTGGCGC GAACCAGCAC GGGACTTACC ACGAGGATGT TCACACGGCG 
CCAGTTTTAC CGGTGGGGCA AAATCCGTTT TTAGCGTCGA TGAGATCGCC ACAGCACGTA 
CAGAGATACG GCCCAACGGT TCAGATGTAC GCGGCACAGT TCGGACAAGG AGGGAACCAT 
TATGTCGGTG GGACTATGCC GCCTGGATAT GGACAGTCGT CGGGGAGAAG TACGCCTAGC 
CCTGCTCCAA CACCCCCTCC CCCGGGAAAC CAGCAACAGA TCTCCCCCCA GACCCCTCAG 
ACCCCGCCCC AGGTTCACTC CCCGCCCCCC TCACAGATGC ACACCCCCTC CCCCCAGGAA 
CAGAGACAAT CCCGCAGCCA GACCAACTCC CCCTTCCCCC CCATGTACCA GCAGCTCACC 
TCCCCTCAGC AACAATACAT GTACTTCCCA CAGCTCCCCT ACCAGATGAT GTCCCCACCC 
CAGGGGTACC CTCCGCAGGG GTCCTTCCCC TCCCCCCAGG GGCAGTACGG CGGGGTCTTC 
ACCTCCCCCC AGGGGCAGCC CCTTCCGGTG GTGCCGGGGT TTTACCAGCC GTACTCGCAA 
GGACCTCAGC AAGGACAGTT CATGTCGGGT CAGTGGCCTC AGGTGACCCC GCCGGGGTAT 
AACCCCATGT ACGACCGCAG GATGTCCGGC GGGAGCGAGA GTTCAGGTCA GGGGTCACCT 
TCCCACGCTC CTCAGCTGCC GCCGTGGAAC TTTCAACAAC AGCAGCAGCA ACAGCAGGCG 
TGGCACGGAG GTCAAGGTCA AATGCACGGG GGTCAAGGTC AAATGCACGG AGGTCAAGGT 
CAAATGCACG GAGGTCAAGG TCAAATGTTT CCAAGGGCGC ACAGCCCGTA CCACAGAACG 
GACTCCCCGC GCGGCGGGCA CTTAGACGCC CACTGGTCGC CCAAACATTC GCCACGCAGC 
CAGAGCCCCA GCCCAGGGAG GTCAGGGTGG GGTCACGGGG CGACGCCGCC GCACCAGTAC 
ACCCCTCCCG GGCAGGGTGG GCAGCCCCGC CACCACGGCC CCTACCAGCG CTCGCTCTCC 
TCACAGAACC ACCAGTATTC CAGCCTCAAC CTCCCCCCGC ACGGCGGCTA CCACCCCCCA 
CAGCCGCCCA ACAAAACTCA CGACTCACCC CGAAGCTCCT TAGTGTTCAC CCAGCCCCTC 
CACGTCCCCA CACACTCCAC CTACTCCTCC CCAGGCCGAC CAGCCATGTC TGTCACAACA 
CCAACCTCCA ACAACTCCAC ACCCACGCCA ACAAAAACAC CCAGCCAGCC CAACCTGCTC 
ACACCCGAGC ACCCGGAGGA GCTAAGGTCG GAAACGGGCA CACCCCCGCC CCCGATTCTC 
CCCGTCATCT CCCCAGCATC CAGCCATAGC AGCCTGTCGT CAGCAGAGAG CAGAGACCGT 
GGACAGAGGC CCAGCACTAG TAATGACAAC TATGCATACG AACAAGCGTT GCTGCTTCAC 
CAGCGGGCGC GGATGGAGAA GTTGAAGAAG GACTGGAACG GGGAGAAGCA GAGACTCGCG 
CAGGTCAGAG TAGAGGTCGA CGAGATGGAA CGTGACCTTC AGGACCGCAG AAAACGCAAC 
GCGACCTCCA AATGTCCCAC GGTAGAAGAG ATAGTGCGTA AGCGGGAACA CAACAGGCAG 
CTGCAGATAG AGATAGACTG TATGACAAAG GAGACTGACC TGCTACAGCA GAGTAGATAC 
TACGTATCCG ACCCGGTGGA CGTGCCGAAC TTCTACGCTA ACCTGGGCGC CACGGGACAG 
CTGGGTCCGG TCCCCCCGCC TAGCGCCGCC TCACAGCATA GACGAAACCT GTCCAGCTCC 
ACGGCAGCTG AAGGTGGTGT GACAGAAAGG GAGGGGGAGG GGGGTTGGCA GATGGTGAAC 
TATTCAGCAG AAGCGCCCAA CCCCAGCGCA GCCCCAGAGG AGGCGGAGGG TCCGCAGTGG 
AACTGTCGAG CCTGTACCTT CCTCAACCAC CCACAGCTAG ACAAATGTGA AATCTGCGAG 
ATGCCCAGAG TTATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC109488122 ( XM_019792285.1 ) cDNA ORF clone, Branchiostoma belcheri(Belcher's lancelet) -> XP_019647844.1 Branchiostoma belcheri TGF-beta-activated kinase 1 and MAP3K7-binding protein 3-like (LOC109488122), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBr198361
Clone ID Related Accession (Same CDS sequence)	XM_019792284.1 , XM_019792285.1
Accession Version	XM_019792285.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2358bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-12-27
Organism	Branchiostoma belcheri(Belcher's lancelet)
Product	TGF-beta-activated kinase 1 and MAP3K7-binding protein 3-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017804587.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Branchiostoma belcheri Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCAGG GCGTGGCCGG TAGCCATGGC AACATGCGCA TGTTCGAGGA GCTGTGCCGA 
CGATTCCAGG AGGTGCCGCA GAACGTGGTG GTGGACGTCA TGAGAAAGAA CGGGAGTAAC 
TTCGAGCTGT GCTGCCAGGA GCTGCAGCAG GAGAGTGGGA AGTATCTGTA CGGAGACTTC 
CAGACAGCGG CGGCGGGGGA AAACGACATG ATGAGCAACC ACATGAAACA GCTCAACATC 
GGCGGGAAAC AACCACCAGA CGACCACAGA CACGGGTTAC ACCATTGCGC GAGCACCCCC 
GCGTTTCTAC CTAGTGGCGC GAACCAGCAC GGGACTTACC ACGAGGATGT TCACACGGCG 
CCAGTTTTAC CGGTGGGGCA AAATCCGTTT TTAGCGTCGA TGAGATCGCC ACAGCACGTA 
CAGAGATACG GCCCAACGGT TCAGATGTAC GCGGCACAGT TCGGACAAGG AGGGAACCAT 
TATGTCGGTG GGACTATGCC GCCTGGATAT GGACAGTCGT CGGGGAGAAG TACGCCTAGC 
CCTGCTCCAA CACCCCCTCC CCCGGGAAAC CAGCAACAGA TCTCCCCCCA GACCCCTCAG 
ACCCCGCCCC AGGTTCACTC CCCGCCCCCC TCACAGATGC ACACCCCCTC CCCCCAGGAA 
CAGAGACAAT CCCGCAGCCA GACCAACTCC CCCTTCCCCC CCATGTACCA GCAGCTCACC 
TCCCCTCAGC AACAATACAT GTACTTCCCA CAGCTCCCCT ACCAGATGAT GTCCCCACCC 
CAGGGGTACC CTCCGCAGGG GTCCTTCCCC TCCCCCCAGG GGCAGTACGG CGGGGTCTTC 
ACCTCCCCCC AGGGGCAGCC CCTTCCGGTG GTGCCGGGGT TTTACCAGCC GTACTCGCAA 
GGACCTCAGC AAGGACAGTT CATGTCGGGT CAGTGGCCTC AGGTGACCCC GCCGGGGTAT 
AACCCCATGT ACGACCGCAG GATGTCCGGC GGGAGCGAGA GTTCAGGTCA GGGGTCACCT 
TCCCACGCTC CTCAGCTGCC GCCGTGGAAC TTTCAACAAC AGCAGCAGCA ACAGCAGGCG 
TGGCACGGAG GTCAAGGTCA AATGCACGGG GGTCAAGGTC AAATGCACGG AGGTCAAGGT 
CAAATGCACG GAGGTCAAGG TCAAATGTTT CCAAGGGCGC ACAGCCCGTA CCACAGAACG 
GACTCCCCGC GCGGCGGGCA CTTAGACGCC CACTGGTCGC CCAAACATTC GCCACGCAGC 
CAGAGCCCCA GCCCAGGGAG GTCAGGGTGG GGTCACGGGG CGACGCCGCC GCACCAGTAC 
ACCCCTCCCG GGCAGGGTGG GCAGCCCCGC CACCACGGCC CCTACCAGCG CTCGCTCTCC 
TCACAGAACC ACCAGTATTC CAGCCTCAAC CTCCCCCCGC ACGGCGGCTA CCACCCCCCA 
CAGCCGCCCA ACAAAACTCA CGACTCACCC CGAAGCTCCT TAGTGTTCAC CCAGCCCCTC 
CACGTCCCCA CACACTCCAC CTACTCCTCC CCAGGCCGAC CAGCCATGTC TGTCACAACA 
CCAACCTCCA ACAACTCCAC ACCCACGCCA ACAAAAACAC CCAGCCAGCC CAACCTGCTC 
ACACCCGAGC ACCCGGAGGA GCTAAGGTCG GAAACGGGCA CACCCCCGCC CCCGATTCTC 
CCCGTCATCT CCCCAGCATC CAGCCATAGC AGCCTGTCGT CAGCAGAGAG CAGAGACCGT 
GGACAGAGGC CCAGCACTAG TAATGACAAC TATGCATACG AACAAGCGTT GCTGCTTCAC 
CAGCGGGCGC GGATGGAGAA GTTGAAGAAG GACTGGAACG GGGAGAAGCA GAGACTCGCG 
CAGGTCAGAG TAGAGGTCGA CGAGATGGAA CGTGACCTTC AGGACCGCAG AAAACGCAAC 
GCGACCTCCA AATGTCCCAC GGTAGAAGAG ATAGTGCGTA AGCGGGAACA CAACAGGCAG 
CTGCAGATAG AGATAGACTG TATGACAAAG GAGACTGACC TGCTACAGCA GAGTAGATAC 
TACGTATCCG ACCCGGTGGA CGTGCCGAAC TTCTACGCTA ACCTGGGCGC CACGGGACAG 
CTGGGTCCGG TCCCCCCGCC TAGCGCCGCC TCACAGCATA GACGAAACCT GTCCAGCTCC 
ACGGCAGCTG AAGGTGGTGT GACAGAAAGG GAGGGGGAGG GGGGTTGGCA GATGGTGAAC 
TATTCAGCAG AAGCGCCCAA CCCCAGCGCA GCCCCAGAGG AGGCGGAGGG TCCGCAGTGG 
AACTGTCGAG CCTGTACCTT CCTCAACCAC CCACAGCTAG ACAAATGTGA AATCTGCGAG 
ATGCCCAGAG TTATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019647844.1
CDS	203..2560
Translation	MAQGVAGSHGNMRMFEELCRRFQEVPQNVVVDVMRKNGSNFELCCQELQQESGKYLYGDFQTAAAGENDMMSNHMKQLNIGGKQPPDDHRHGLHHCASTPAFLPSGANQHGTYHEDVHTAPVLPVGQNPFLASMRSPQHVQRYGPTVQMYAAQFGQGGNHYVGGTMPPGYGQSSGRSTPSPAPTPPPPGNQQQISPQTPQTPPQVHSPPPSQMHTPSPQEQRQSRSQTNSPFPPMYQQLTSPQQQYMYFPQLPYQMMSPPQGYPPQGSFPSPQGQYGGVFTSPQGQPLPVVPGFYQPYSQGPQQGQFMSGQWPQVTPPGYNPMYDRRMSGGSESSGQGSPSHAPQLPPWNFQQQQQQQQAWHGGQGQMHGGQGQMHGGQGQMHGGQGQMFPRAHSPYHRTDSPRGGHLDAHWSPKHSPRSQSPSPGRSGWGHGATPPHQYTPPGQGGQPRHHGPYQRSLSSQNHQYSSLNLPPHGGYHPPQPPNKTHDSPRSSLVFTQPLHVPTHSTYSSPGRPAMSVTTPTSNNSTPTPTKTPSQPNLLTPEHPEELRSETGTPPPPILPVISPASSHSSLSSAESRDRGQRPSTSNDNYAYEQALLLHQRARMEKLKKDWNGEKQRLAQVRVEVDEMERDLQDRRKRNATSKCPTVEEIVRKREHNRQLQIEIDCMTKETDLLQQSRYYVSDPVDVPNFYANLGATGQLGPVPPPSAASQHRRNLSSSTAAEGGVTEREGEGGWQMVNYSAEAPNPSAAPEEAEGPQWNCRACTFLNHPQLDKCEICEMPRVI

Target ORF information:

RefSeq Version	XM_019792285.1
Organism	Branchiostoma belcheri(Belcher's lancelet)
Definition	Branchiostoma belcheri TGF-beta-activated kinase 1 and MAP3K7-binding protein 3-like (LOC109488122), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019792285.1

ORF Insert Sequence:

ATGGCGCAGG GCGTGGCCGG TAGCCATGGC AACATGCGCA TGTTCGAGGA GCTGTGCCGA 
CGATTCCAGG AGGTGCCGCA GAACGTGGTG GTGGACGTCA TGAGAAAGAA CGGGAGTAAC 
TTCGAGCTGT GCTGCCAGGA GCTGCAGCAG GAGAGTGGGA AGTATCTGTA CGGAGACTTC 
CAGACAGCGG CGGCGGGGGA AAACGACATG ATGAGCAACC ACATGAAACA GCTCAACATC 
GGCGGGAAAC AACCACCAGA CGACCACAGA CACGGGTTAC ACCATTGCGC GAGCACCCCC 
GCGTTTCTAC CTAGTGGCGC GAACCAGCAC GGGACTTACC ACGAGGATGT TCACACGGCG 
CCAGTTTTAC CGGTGGGGCA AAATCCGTTT TTAGCGTCGA TGAGATCGCC ACAGCACGTA 
CAGAGATACG GCCCAACGGT TCAGATGTAC GCGGCACAGT TCGGACAAGG AGGGAACCAT 
TATGTCGGTG GGACTATGCC GCCTGGATAT GGACAGTCGT CGGGGAGAAG TACGCCTAGC 
CCTGCTCCAA CACCCCCTCC CCCGGGAAAC CAGCAACAGA TCTCCCCCCA GACCCCTCAG 
ACCCCGCCCC AGGTTCACTC CCCGCCCCCC TCACAGATGC ACACCCCCTC CCCCCAGGAA 
CAGAGACAAT CCCGCAGCCA GACCAACTCC CCCTTCCCCC CCATGTACCA GCAGCTCACC 
TCCCCTCAGC AACAATACAT GTACTTCCCA CAGCTCCCCT ACCAGATGAT GTCCCCACCC 
CAGGGGTACC CTCCGCAGGG GTCCTTCCCC TCCCCCCAGG GGCAGTACGG CGGGGTCTTC 
ACCTCCCCCC AGGGGCAGCC CCTTCCGGTG GTGCCGGGGT TTTACCAGCC GTACTCGCAA 
GGACCTCAGC AAGGACAGTT CATGTCGGGT CAGTGGCCTC AGGTGACCCC GCCGGGGTAT 
AACCCCATGT ACGACCGCAG GATGTCCGGC GGGAGCGAGA GTTCAGGTCA GGGGTCACCT 
TCCCACGCTC CTCAGCTGCC GCCGTGGAAC TTTCAACAAC AGCAGCAGCA ACAGCAGGCG 
TGGCACGGAG GTCAAGGTCA AATGCACGGG GGTCAAGGTC AAATGCACGG AGGTCAAGGT 
CAAATGCACG GAGGTCAAGG TCAAATGTTT CCAAGGGCGC ACAGCCCGTA CCACAGAACG 
GACTCCCCGC GCGGCGGGCA CTTAGACGCC CACTGGTCGC CCAAACATTC GCCACGCAGC 
CAGAGCCCCA GCCCAGGGAG GTCAGGGTGG GGTCACGGGG CGACGCCGCC GCACCAGTAC 
ACCCCTCCCG GGCAGGGTGG GCAGCCCCGC CACCACGGCC CCTACCAGCG CTCGCTCTCC 
TCACAGAACC ACCAGTATTC CAGCCTCAAC CTCCCCCCGC ACGGCGGCTA CCACCCCCCA 
CAGCCGCCCA ACAAAACTCA CGACTCACCC CGAAGCTCCT TAGTGTTCAC CCAGCCCCTC 
CACGTCCCCA CACACTCCAC CTACTCCTCC CCAGGCCGAC CAGCCATGTC TGTCACAACA 
CCAACCTCCA ACAACTCCAC ACCCACGCCA ACAAAAACAC CCAGCCAGCC CAACCTGCTC 
ACACCCGAGC ACCCGGAGGA GCTAAGGTCG GAAACGGGCA CACCCCCGCC CCCGATTCTC 
CCCGTCATCT CCCCAGCATC CAGCCATAGC AGCCTGTCGT CAGCAGAGAG CAGAGACCGT 
GGACAGAGGC CCAGCACTAG TAATGACAAC TATGCATACG AACAAGCGTT GCTGCTTCAC 
CAGCGGGCGC GGATGGAGAA GTTGAAGAAG GACTGGAACG GGGAGAAGCA GAGACTCGCG 
CAGGTCAGAG TAGAGGTCGA CGAGATGGAA CGTGACCTTC AGGACCGCAG AAAACGCAAC 
GCGACCTCCA AATGTCCCAC GGTAGAAGAG ATAGTGCGTA AGCGGGAACA CAACAGGCAG 
CTGCAGATAG AGATAGACTG TATGACAAAG GAGACTGACC TGCTACAGCA GAGTAGATAC 
TACGTATCCG ACCCGGTGGA CGTGCCGAAC TTCTACGCTA ACCTGGGCGC CACGGGACAG 
CTGGGTCCGG TCCCCCCGCC TAGCGCCGCC TCACAGCATA GACGAAACCT GTCCAGCTCC 
ACGGCAGCTG AAGGTGGTGT GACAGAAAGG GAGGGGGAGG GGGGTTGGCA GATGGTGAAC 
TATTCAGCAG AAGCGCCCAA CCCCAGCGCA GCCCCAGAGG AGGCGGAGGG TCCGCAGTGG 
AACTGTCGAG CCTGTACCTT CCTCAACCAC CCACAGCTAG ACAAATGTGA AATCTGCGAG 
ATGCCCAGAG TTATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC109488122 ( XM_019792286.1 ) cDNA ORF clone, Branchiostoma belcheri(Belcher's lancelet) -> XP_019647845.1 Branchiostoma belcheri TGF-beta-activated kinase 1 and MAP3K7-binding protein 3-like (LOC109488122), transcript variant X3, mRNA.

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CloneID	OBr198362
Clone ID Related Accession (Same CDS sequence)	XM_019792286.1
Accession Version	XM_019792286.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2271bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-12-27
Organism	Branchiostoma belcheri(Belcher's lancelet)
Product	TGF-beta-activated kinase 1 and MAP3K7-binding protein 3-like isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017804587.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Branchiostoma belcheri Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCAGG GCGTGGCCGG TAGCCATGGC AACATGCGCA TGTTCGAGGA GCTGTGCCGA 
CGATTCCAGG AGGTGCCGCA GAACGTGGTG GTGGACGTCA TGAGAAAGAA CGGGAGTAAC 
TTCGAGCTGT GCTGCCAGGA GCTGCAGCAG GAGAGTGGGA AGTATCTGTA CGGAGACTTC 
CAGACAGCGG CGGCGGGGGA AAACGACATG ATGAGCAACC ACATGAAACA GCTCAACATC 
GGCGGGAAAC AACCACCAGA CGACCACAGA CACGGGTTAC ACCATTGCGC GAGCACCCCC 
GCGTTTCTAC CTAGTGGCGC GAACCAGCAC GGGACTTACC ACGAGGATGT TCACACGGCG 
CCAGTTTTAC CGGTGGGGCA AAATCCGTTT TTAGCGTCGA TGAGATCGCC ACAGCACGTA 
CAGAGATACG GCCCAACGGT TCAGATGTAC GCGGCACAGT TCGGACAAGG AGGGAACCAT 
TATGTCGGTG GGACTATGCC GCCTGGATAT GGACAGTCGT CGGGGAGAAG TACGCCTAGC 
CCTGCTCCAA CACCCCCTCC CCCGGGAAAC CAGCAACAGA TCTCCCCCCA GACCCCTCAG 
ACCCCGCCCC AGGTTCACTC CCCGCCCCCC TCACAGATGC ACACCCCCTC CCCCCAGGAA 
CAGAGACAAT CCCGCAGCCA GACCAACTCC CCCTTCCCCC CCATGTACCA GCAGCTCACC 
TCCCCTCAGC AACAATACAT GTACTTCCCA CAGCTCCCCT ACCAGATGAT GTCCCCACCC 
CAGGGGTACC CTCCGCAGGG GTCCTTCCCC TCCCCCCAGG GGCAGTACGG CGGGGTCTTC 
ACCTCCCCCC AGGGGCAGCC CCTTCCGGTG GTGCCGGGGT TTTACCAGCC GTACTCGCAA 
GGACCTCAGC AAGGACAGTT CATGTCGGGT CAGTGGCCTC AGGTGACCCC GCCGGGGTAT 
AACCCCATGT ACGACCGCAG GATGTCCGGC GGGAGCGAGA GTTCAGGTCA GGGGTCACCT 
TCCCACGCTC CTCAGCTGCC GCCGTGGAAC TTTCAACAAC AGCAGCAGCA ACAGCAGGCG 
TGGCACGGAG GTCAAGGTCA AATGCACGGG GGTCAAGGTC AAATGCACGG AGGTCAAGGT 
CAAATGCACG GAGGTCAAGG TCAAATGTTT CCAAGGGCGC ACAGCCCGTA CCACAGAACG 
GACTCCCCGC GCGGCGGGCA CTTAGACGCC CACTGGTCGC CCAAACATTC GCCACGCAGC 
CAGAGCCCCA GCCCAGGGAG GTCAGGGTGG GGTCACGGGG CGACGCCGCC GCACCAGTAC 
ACCCCTCCCG GGCAGGGTGG GCAGCCCCGC CACCACGGCC CCTACCAGCG CTCGCTCTCC 
TCACAGAACC ACCAGTATTC CAGCCTCAAC CTCCCCCCGC ACGGCGGCTA CCACCCCCCA 
CAGCCGCCCA ACAAAACTCA CGACTCACCC CGAAGCTCCT TAGTGTTCAC CCAGCCCCTC 
CACGTCCCCA CACACTCCAC CTACTCCTCC CCAGGCCGAC CAGCCATGTC TGTCACAACA 
CCAACCTCCA ACAACTCCAC ACCCACGCCA ACAAAAACAC CCAGCCAGCC CAACCTGCTC 
ACACCCGAGC ACCCGGAGGA GCTAAGGTCG GAAACGGGCA CACCCCCGCC CCCGATTCTC 
CCCGTCATCT CCCCAGCATC CAGCCATAGC AGCCTGTCGT CAGCAGAGAG CAGAGACCGT 
GGACAGAGGC CCAGCACTAG TAATGACAAC TATGCATACG AACAAGCGTT GCTGCTTCAC 
CAGCGGGCGC GGATGGAGAA GTTGAAGAAG GACTGGAACG GGGAGAAGCA GAGACTCGCG 
CAGGTCAGAG TAGAGGTCGA CGAGATGGAA CGTGACCTTC AGGACCGCAG AAAACGCAAC 
GCGACCTCCA AATGTCCCAC GGTAGAAGAG ATAGTGCGTA AGCGGGAACA CAACAGGCAG 
CTGCAGATAG AGATAGACTG TATGACAAAG GAGACTGACC TGCTACAGCA GAGTAGATAC 
TACGTATCCG ACCCGGTGGA CGTGCCGAAC TTCTACGCTA ACCTGGGCGC CACGGGACAG 
CTGGGTCCGG TCCCCCCGCC TAGCGCCGCC TCACAGCATA GACAAGCGCC CAACCCCAGC 
GCAGCCCCAG AGGAGGCGGA GGGTCCGCAG TGGAACTGTC GAGCCTGTAC CTTCCTCAAC 
CACCCACAGC TAGACAAATG TGAAATCTGC GAGATGCCCA GAGTTATATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019647845.1
CDS	240..2510
Translation	MAQGVAGSHGNMRMFEELCRRFQEVPQNVVVDVMRKNGSNFELCCQELQQESGKYLYGDFQTAAAGENDMMSNHMKQLNIGGKQPPDDHRHGLHHCASTPAFLPSGANQHGTYHEDVHTAPVLPVGQNPFLASMRSPQHVQRYGPTVQMYAAQFGQGGNHYVGGTMPPGYGQSSGRSTPSPAPTPPPPGNQQQISPQTPQTPPQVHSPPPSQMHTPSPQEQRQSRSQTNSPFPPMYQQLTSPQQQYMYFPQLPYQMMSPPQGYPPQGSFPSPQGQYGGVFTSPQGQPLPVVPGFYQPYSQGPQQGQFMSGQWPQVTPPGYNPMYDRRMSGGSESSGQGSPSHAPQLPPWNFQQQQQQQQAWHGGQGQMHGGQGQMHGGQGQMHGGQGQMFPRAHSPYHRTDSPRGGHLDAHWSPKHSPRSQSPSPGRSGWGHGATPPHQYTPPGQGGQPRHHGPYQRSLSSQNHQYSSLNLPPHGGYHPPQPPNKTHDSPRSSLVFTQPLHVPTHSTYSSPGRPAMSVTTPTSNNSTPTPTKTPSQPNLLTPEHPEELRSETGTPPPPILPVISPASSHSSLSSAESRDRGQRPSTSNDNYAYEQALLLHQRARMEKLKKDWNGEKQRLAQVRVEVDEMERDLQDRRKRNATSKCPTVEEIVRKREHNRQLQIEIDCMTKETDLLQQSRYYVSDPVDVPNFYANLGATGQLGPVPPPSAASQHRQAPNPSAAPEEAEGPQWNCRACTFLNHPQLDKCEICEMPRVI

Target ORF information:

RefSeq Version	XM_019792286.1
Organism	Branchiostoma belcheri(Belcher's lancelet)
Definition	Branchiostoma belcheri TGF-beta-activated kinase 1 and MAP3K7-binding protein 3-like (LOC109488122), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019792286.1

ORF Insert Sequence:

ATGGCGCAGG GCGTGGCCGG TAGCCATGGC AACATGCGCA TGTTCGAGGA GCTGTGCCGA 
CGATTCCAGG AGGTGCCGCA GAACGTGGTG GTGGACGTCA TGAGAAAGAA CGGGAGTAAC 
TTCGAGCTGT GCTGCCAGGA GCTGCAGCAG GAGAGTGGGA AGTATCTGTA CGGAGACTTC 
CAGACAGCGG CGGCGGGGGA AAACGACATG ATGAGCAACC ACATGAAACA GCTCAACATC 
GGCGGGAAAC AACCACCAGA CGACCACAGA CACGGGTTAC ACCATTGCGC GAGCACCCCC 
GCGTTTCTAC CTAGTGGCGC GAACCAGCAC GGGACTTACC ACGAGGATGT TCACACGGCG 
CCAGTTTTAC CGGTGGGGCA AAATCCGTTT TTAGCGTCGA TGAGATCGCC ACAGCACGTA 
CAGAGATACG GCCCAACGGT TCAGATGTAC GCGGCACAGT TCGGACAAGG AGGGAACCAT 
TATGTCGGTG GGACTATGCC GCCTGGATAT GGACAGTCGT CGGGGAGAAG TACGCCTAGC 
CCTGCTCCAA CACCCCCTCC CCCGGGAAAC CAGCAACAGA TCTCCCCCCA GACCCCTCAG 
ACCCCGCCCC AGGTTCACTC CCCGCCCCCC TCACAGATGC ACACCCCCTC CCCCCAGGAA 
CAGAGACAAT CCCGCAGCCA GACCAACTCC CCCTTCCCCC CCATGTACCA GCAGCTCACC 
TCCCCTCAGC AACAATACAT GTACTTCCCA CAGCTCCCCT ACCAGATGAT GTCCCCACCC 
CAGGGGTACC CTCCGCAGGG GTCCTTCCCC TCCCCCCAGG GGCAGTACGG CGGGGTCTTC 
ACCTCCCCCC AGGGGCAGCC CCTTCCGGTG GTGCCGGGGT TTTACCAGCC GTACTCGCAA 
GGACCTCAGC AAGGACAGTT CATGTCGGGT CAGTGGCCTC AGGTGACCCC GCCGGGGTAT 
AACCCCATGT ACGACCGCAG GATGTCCGGC GGGAGCGAGA GTTCAGGTCA GGGGTCACCT 
TCCCACGCTC CTCAGCTGCC GCCGTGGAAC TTTCAACAAC AGCAGCAGCA ACAGCAGGCG 
TGGCACGGAG GTCAAGGTCA AATGCACGGG GGTCAAGGTC AAATGCACGG AGGTCAAGGT 
CAAATGCACG GAGGTCAAGG TCAAATGTTT CCAAGGGCGC ACAGCCCGTA CCACAGAACG 
GACTCCCCGC GCGGCGGGCA CTTAGACGCC CACTGGTCGC CCAAACATTC GCCACGCAGC 
CAGAGCCCCA GCCCAGGGAG GTCAGGGTGG GGTCACGGGG CGACGCCGCC GCACCAGTAC 
ACCCCTCCCG GGCAGGGTGG GCAGCCCCGC CACCACGGCC CCTACCAGCG CTCGCTCTCC 
TCACAGAACC ACCAGTATTC CAGCCTCAAC CTCCCCCCGC ACGGCGGCTA CCACCCCCCA 
CAGCCGCCCA ACAAAACTCA CGACTCACCC CGAAGCTCCT TAGTGTTCAC CCAGCCCCTC 
CACGTCCCCA CACACTCCAC CTACTCCTCC CCAGGCCGAC CAGCCATGTC TGTCACAACA 
CCAACCTCCA ACAACTCCAC ACCCACGCCA ACAAAAACAC CCAGCCAGCC CAACCTGCTC 
ACACCCGAGC ACCCGGAGGA GCTAAGGTCG GAAACGGGCA CACCCCCGCC CCCGATTCTC 
CCCGTCATCT CCCCAGCATC CAGCCATAGC AGCCTGTCGT CAGCAGAGAG CAGAGACCGT 
GGACAGAGGC CCAGCACTAG TAATGACAAC TATGCATACG AACAAGCGTT GCTGCTTCAC 
CAGCGGGCGC GGATGGAGAA GTTGAAGAAG GACTGGAACG GGGAGAAGCA GAGACTCGCG 
CAGGTCAGAG TAGAGGTCGA CGAGATGGAA CGTGACCTTC AGGACCGCAG AAAACGCAAC 
GCGACCTCCA AATGTCCCAC GGTAGAAGAG ATAGTGCGTA AGCGGGAACA CAACAGGCAG 
CTGCAGATAG AGATAGACTG TATGACAAAG GAGACTGACC TGCTACAGCA GAGTAGATAC 
TACGTATCCG ACCCGGTGGA CGTGCCGAAC TTCTACGCTA ACCTGGGCGC CACGGGACAG 
CTGGGTCCGG TCCCCCCGCC TAGCGCCGCC TCACAGCATA GACAAGCGCC CAACCCCAGC 
GCAGCCCCAG AGGAGGCGGA GGGTCCGCAG TGGAACTGTC GAGCCTGTAC CTTCCTCAAC 
CACCCACAGC TAGACAAATG TGAAATCTGC GAGATGCCCA GAGTTATATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC109488122 ( XM_019792287.1 ) cDNA ORF clone, Branchiostoma belcheri(Belcher's lancelet) -> XP_019647846.1 Branchiostoma belcheri TGF-beta-activated kinase 1 and MAP3K7-binding protein 3-like (LOC109488122), transcript variant X4, mRNA.

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CloneID	OBr198363
Clone ID Related Accession (Same CDS sequence)	XM_019792287.1
Accession Version	XM_019792287.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2268bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-12-27
Organism	Branchiostoma belcheri(Belcher's lancelet)
Product	TGF-beta-activated kinase 1 and MAP3K7-binding protein 3-like isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017804587.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Branchiostoma belcheri Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCAGG GCGTGGCCGG TAGCCATGGC AACATGCGCA TGTTCGAGGA GCTGTGCCGA 
CGATTCCAGG AGGTGCCGCA GAACGTGGTG GTGGACGTCA TGAGAAAGAA CGGGAGTAAC 
TTCGAGCTGT GCTGCCAGGA GCTGCAGCAG GAGAGTGGGA AGTATCTGTA CGGAGACTTC 
CAGACAGCGG CGGCGGGGGA AAACGACATG ATGAGCAACC ACATGAAACA GCTCAACATC 
GGCGGGAAAC AACCACCAGA CGACCACAGA CACGGGTTAC ACCATTGCGC GAGCACCCCC 
GCGTTTCTAC CTAGTGGCGC GAACCAGCAC GGGACTTACC ACGAGGATGT TCACACGGCG 
CCAGTTTTAC CGGTGGGGCA AAATCCGTTT TTAGCGTCGA TGAGATCGCC ACAGCACGTA 
CAGAGATACG GCCCAACGGT TCAGATGTAC GCGGCACAGT TCGGACAAGG AGGGAACCAT 
TATGTCGGTG GGACTATGCC GCCTGGATAT GGACAGTCGT CGGGGAGAAG TACGCCTAGC 
CCTGCTCCAA CACCCCCTCC CCCGGGAAAC CAGCAACAGA TCTCCCCCCA GACCCCTCAG 
ACCCCGCCCC AGGTTCACTC CCCGCCCCCC TCACAGATGC ACACCCCCTC CCCCCAGGAA 
CAGAGACAAT CCCGCAGCCA GACCAACTCC CCCTTCCCCC CCATGTACCA GCAGCTCACC 
TCCCCTCAGC AACAATACAT GTACTTCCCA CAGCTCCCCT ACCAGATGAT GTCCCCACCC 
CAGGGGTACC CTCCGCAGGG GTCCTTCCCC TCCCCCCAGG GGCAGTACGG CGGGGTCTTC 
ACCTCCCCCC AGGGGCAGCC CCTTCCGGTG GTGCCGGGGT TTTACCAGCC GTACTCGCAA 
GGACCTCAGC AAGGACAGTT CATGTCGGGT CAGTGGCCTC AGGTGACCCC GCCGGGGTAT 
AACCCCATGT ACGACCGCAG GATGTCCGGC GGGAGCGAGA GTTCAGGTCA GGGGTCACCT 
TCCCACGCTC CTCAGCTGCC GCCGTGGAAC TTTCAACAAC AGCAGCAGCA ACAGCAGGCG 
TGGCACGGAG GTCAAGGTCA AATGCACGGG GGTCAAGGTC AAATGCACGG AGGTCAAGGT 
CAAATGCACG GAGGTCAAGG TCAAATGTTT CCAAGGGCGC ACAGCCCGTA CCACAGAACG 
GACTCCCCGC GCGGCGGGCA CTTAGACGCC CACTGGTCGC CCAAACATTC GCCACGCAGC 
CAGAGCCCCA GCCCAGGGAG GTCAGGGTGG GGTCACGGGG CGACGCCGCC GCACCAGTAC 
ACCCCTCCCG GGCAGGGTGG GCAGCCCCGC CACCACGGCC CCTACCAGCG CTCGCTCTCC 
TCACAGAACC ACCAGTATTC CAGCCTCAAC CTCCCCCCGC ACGGCGGCTA CCACCCCCCA 
CAGCCGCCCA ACAAAACTCA CGACTCACCC CGAAGCTCCT TAGTGTTCAC CCAGCCCCTC 
CACGTCCCCA CACACTCCAC CTACTCCTCC CCAGGCCGAC CAGCCATGTC TGTCACAACA 
CCAACCTCCA ACAACTCCAC ACCCACGCCA ACAAAAACAC CCAGCCAGCC CAACCTGCTC 
ACACCCGAGC ACCCGGAGGA GCTAAGGTCG GAAACGGGCA CACCCCCGCC CCCGATTCTC 
CCCGTCATCT CCCCAGCATC CAGCCATAGC AGCCTGTCGT CAGCAGAGAG CAGAGACCGT 
GGACAGAGGC CCAGCACTAG TAATGACAAC TATGCATACG AACAAGCGTT GCTGCTTCAC 
CAGCGGGCGC GGATGGAGAA GTTGAAGAAG GACTGGAACG GGGAGAAGCA GAGACTCGCG 
CAGGTCAGAG TAGAGGTCGA CGAGATGGAA CGTGACCTTC AGGACCGCAG AAAACGCAAC 
GCGACCTCCA AATGTCCCAC GGTAGAAGAG ATAGTGCGTA AGCGGGAACA CAACAGGCAG 
CTGCAGATAG AGATAGACTG TATGACAAAG GAGACTGACC TGCTACAGCA GAGTAGATAC 
TACGTATCCG ACCCGGTGGA CGTGCCGAAC TTCTACGCTA ACCTGGGCGC CACGGGACAG 
CTGGGTCCGG TCCCCCCGCC TAGCGCCGCC TCACAGCATA GACCGCCCAA CCCCAGCGCA 
GCCCCAGAGG AGGCGGAGGG TCCGCAGTGG AACTGTCGAG CCTGTACCTT CCTCAACCAC 
CCACAGCTAG ACAAATGTGA AATCTGCGAG ATGCCCAGAG TTATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019647846.1
CDS	240..2507
Translation	MAQGVAGSHGNMRMFEELCRRFQEVPQNVVVDVMRKNGSNFELCCQELQQESGKYLYGDFQTAAAGENDMMSNHMKQLNIGGKQPPDDHRHGLHHCASTPAFLPSGANQHGTYHEDVHTAPVLPVGQNPFLASMRSPQHVQRYGPTVQMYAAQFGQGGNHYVGGTMPPGYGQSSGRSTPSPAPTPPPPGNQQQISPQTPQTPPQVHSPPPSQMHTPSPQEQRQSRSQTNSPFPPMYQQLTSPQQQYMYFPQLPYQMMSPPQGYPPQGSFPSPQGQYGGVFTSPQGQPLPVVPGFYQPYSQGPQQGQFMSGQWPQVTPPGYNPMYDRRMSGGSESSGQGSPSHAPQLPPWNFQQQQQQQQAWHGGQGQMHGGQGQMHGGQGQMHGGQGQMFPRAHSPYHRTDSPRGGHLDAHWSPKHSPRSQSPSPGRSGWGHGATPPHQYTPPGQGGQPRHHGPYQRSLSSQNHQYSSLNLPPHGGYHPPQPPNKTHDSPRSSLVFTQPLHVPTHSTYSSPGRPAMSVTTPTSNNSTPTPTKTPSQPNLLTPEHPEELRSETGTPPPPILPVISPASSHSSLSSAESRDRGQRPSTSNDNYAYEQALLLHQRARMEKLKKDWNGEKQRLAQVRVEVDEMERDLQDRRKRNATSKCPTVEEIVRKREHNRQLQIEIDCMTKETDLLQQSRYYVSDPVDVPNFYANLGATGQLGPVPPPSAASQHRPPNPSAAPEEAEGPQWNCRACTFLNHPQLDKCEICEMPRVI

Target ORF information:

RefSeq Version	XM_019792287.1
Organism	Branchiostoma belcheri(Belcher's lancelet)
Definition	Branchiostoma belcheri TGF-beta-activated kinase 1 and MAP3K7-binding protein 3-like (LOC109488122), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019792287.1

ORF Insert Sequence:

ATGGCGCAGG GCGTGGCCGG TAGCCATGGC AACATGCGCA TGTTCGAGGA GCTGTGCCGA 
CGATTCCAGG AGGTGCCGCA GAACGTGGTG GTGGACGTCA TGAGAAAGAA CGGGAGTAAC 
TTCGAGCTGT GCTGCCAGGA GCTGCAGCAG GAGAGTGGGA AGTATCTGTA CGGAGACTTC 
CAGACAGCGG CGGCGGGGGA AAACGACATG ATGAGCAACC ACATGAAACA GCTCAACATC 
GGCGGGAAAC AACCACCAGA CGACCACAGA CACGGGTTAC ACCATTGCGC GAGCACCCCC 
GCGTTTCTAC CTAGTGGCGC GAACCAGCAC GGGACTTACC ACGAGGATGT TCACACGGCG 
CCAGTTTTAC CGGTGGGGCA AAATCCGTTT TTAGCGTCGA TGAGATCGCC ACAGCACGTA 
CAGAGATACG GCCCAACGGT TCAGATGTAC GCGGCACAGT TCGGACAAGG AGGGAACCAT 
TATGTCGGTG GGACTATGCC GCCTGGATAT GGACAGTCGT CGGGGAGAAG TACGCCTAGC 
CCTGCTCCAA CACCCCCTCC CCCGGGAAAC CAGCAACAGA TCTCCCCCCA GACCCCTCAG 
ACCCCGCCCC AGGTTCACTC CCCGCCCCCC TCACAGATGC ACACCCCCTC CCCCCAGGAA 
CAGAGACAAT CCCGCAGCCA GACCAACTCC CCCTTCCCCC CCATGTACCA GCAGCTCACC 
TCCCCTCAGC AACAATACAT GTACTTCCCA CAGCTCCCCT ACCAGATGAT GTCCCCACCC 
CAGGGGTACC CTCCGCAGGG GTCCTTCCCC TCCCCCCAGG GGCAGTACGG CGGGGTCTTC 
ACCTCCCCCC AGGGGCAGCC CCTTCCGGTG GTGCCGGGGT TTTACCAGCC GTACTCGCAA 
GGACCTCAGC AAGGACAGTT CATGTCGGGT CAGTGGCCTC AGGTGACCCC GCCGGGGTAT 
AACCCCATGT ACGACCGCAG GATGTCCGGC GGGAGCGAGA GTTCAGGTCA GGGGTCACCT 
TCCCACGCTC CTCAGCTGCC GCCGTGGAAC TTTCAACAAC AGCAGCAGCA ACAGCAGGCG 
TGGCACGGAG GTCAAGGTCA AATGCACGGG GGTCAAGGTC AAATGCACGG AGGTCAAGGT 
CAAATGCACG GAGGTCAAGG TCAAATGTTT CCAAGGGCGC ACAGCCCGTA CCACAGAACG 
GACTCCCCGC GCGGCGGGCA CTTAGACGCC CACTGGTCGC CCAAACATTC GCCACGCAGC 
CAGAGCCCCA GCCCAGGGAG GTCAGGGTGG GGTCACGGGG CGACGCCGCC GCACCAGTAC 
ACCCCTCCCG GGCAGGGTGG GCAGCCCCGC CACCACGGCC CCTACCAGCG CTCGCTCTCC 
TCACAGAACC ACCAGTATTC CAGCCTCAAC CTCCCCCCGC ACGGCGGCTA CCACCCCCCA 
CAGCCGCCCA ACAAAACTCA CGACTCACCC CGAAGCTCCT TAGTGTTCAC CCAGCCCCTC 
CACGTCCCCA CACACTCCAC CTACTCCTCC CCAGGCCGAC CAGCCATGTC TGTCACAACA 
CCAACCTCCA ACAACTCCAC ACCCACGCCA ACAAAAACAC CCAGCCAGCC CAACCTGCTC 
ACACCCGAGC ACCCGGAGGA GCTAAGGTCG GAAACGGGCA CACCCCCGCC CCCGATTCTC 
CCCGTCATCT CCCCAGCATC CAGCCATAGC AGCCTGTCGT CAGCAGAGAG CAGAGACCGT 
GGACAGAGGC CCAGCACTAG TAATGACAAC TATGCATACG AACAAGCGTT GCTGCTTCAC 
CAGCGGGCGC GGATGGAGAA GTTGAAGAAG GACTGGAACG GGGAGAAGCA GAGACTCGCG 
CAGGTCAGAG TAGAGGTCGA CGAGATGGAA CGTGACCTTC AGGACCGCAG AAAACGCAAC 
GCGACCTCCA AATGTCCCAC GGTAGAAGAG ATAGTGCGTA AGCGGGAACA CAACAGGCAG 
CTGCAGATAG AGATAGACTG TATGACAAAG GAGACTGACC TGCTACAGCA GAGTAGATAC 
TACGTATCCG ACCCGGTGGA CGTGCCGAAC TTCTACGCTA ACCTGGGCGC CACGGGACAG 
CTGGGTCCGG TCCCCCCGCC TAGCGCCGCC TCACAGCATA GACCGCCCAA CCCCAGCGCA 
GCCCCAGAGG AGGCGGAGGG TCCGCAGTGG AACTGTCGAG CCTGTACCTT CCTCAACCAC 
CCACAGCTAG ACAAATGTGA AATCTGCGAG ATGCCCAGAG TTATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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LOC109488122 cDNA ORF clone, Branchiostoma belcheri(Belcher's lancelet)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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見積依頼

Eメール

電話

FAX

オンラインフォーム