CIT gene cDNA ORF clone, Bos taurus(cattle)

mRNA	Protein	Name
XM_015475534.1	XP_015331020.1	citron Rho-interacting kinase
XM_024977735.1	XP_024833503.1	citron Rho-interacting kinase isoform X2
XM_024977737.1	XP_024833505.1	citron Rho-interacting kinase isoform X4
XM_024977738.1	XP_024833506.1	citron Rho-interacting kinase isoform X5
XM_015467110.1	XP_015322596.1	citron Rho-interacting kinase
XM_024977741.1	XP_024833509.1	citron Rho-interacting kinase isoform X8
XM_024977733.1	XP_024833501.1	citron Rho-interacting kinase isoform X1
XM_024977736.1	XP_024833504.1	citron Rho-interacting kinase isoform X3
XM_024977739.1	XP_024833507.1	citron Rho-interacting kinase isoform X6
XM_024977743.1	XP_024833511.1	citron Rho-interacting kinase isoform X9
XM_024977734.1	XP_024833502.1	citron Rho-interacting kinase isoform X1
XM_024977740.1	XP_024833508.1	citron Rho-interacting kinase isoform X7
XM_024977742.1	XP_024833510.1	citron Rho-interacting kinase isoform X9
XM_024977744.1	XP_024833512.1	citron Rho-interacting kinase isoform X10

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa224627	XM_024977739.1 Latest version!	Bos taurus citron rho-interacting serine/threonine kinase (CIT), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa224628	XM_024977740.1 Latest version!	Bos taurus citron rho-interacting serine/threonine kinase (CIT), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa224629	XM_024977741.1 Latest version!	Bos taurus citron rho-interacting serine/threonine kinase (CIT), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa224630	XM_024977743.1 Latest version!	Bos taurus citron rho-interacting serine/threonine kinase (CIT), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBa224630	XM_024977742.1 Latest version!	Bos taurus citron rho-interacting serine/threonine kinase (CIT), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBa224631	XM_024977744.1 Latest version!	Bos taurus citron rho-interacting serine/threonine kinase (CIT), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBa93236	XM_015467110.1 Latest version!	Bos taurus citron rho-interacting serine/threonine kinase (CIT), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBa93236	XM_015475534.1 Latest version!	Bos taurus citron rho-interacting serine/threonine kinase (CIT), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBa224622	XM_024977734.1 Latest version!	Bos taurus citron rho-interacting serine/threonine kinase (CIT), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBa224622	XM_024977733.1 Latest version!	Bos taurus citron rho-interacting serine/threonine kinase (CIT), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBa224623	XM_024977735.1 Latest version!	Bos taurus citron rho-interacting serine/threonine kinase (CIT), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBa224624	XM_024977736.1 Latest version!	Bos taurus citron rho-interacting serine/threonine kinase (CIT), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBa224625	XM_024977737.1 Latest version!	Bos taurus citron rho-interacting serine/threonine kinase (CIT), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBa224626	XM_024977738.1 Latest version!	Bos taurus citron rho-interacting serine/threonine kinase (CIT), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00

CIT ( XM_024977739.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024833507.1 Bos taurus citron rho-interacting serine/threonine kinase (CIT), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OBa224627
Clone ID Related Accession (Same CDS sequence)	XM_024977739.1
Accession Version	XM_024977739.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5880bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	citron Rho-interacting kinase isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGTCA TGAAGAAGAA GGCCTTGTTG GCCCAGGAGC AGGTCTCATA TTTTGAGGAA 
GAACAGAACA TATTATCTCA GAGTACAAGT CCTTGGATCC CCCAGTTGCA GTATGCCTTT 
CAGGACAAAA ATAACCTTTA TCTGGTCATG GAATATCAGC CTGGAGGGGA TTTGCTGTCA 
CTTTTGAATA GATATGAGGA CCAATTAGAT GAAAATATGA TTCAGTTTTA CCTAGCTGAA 
CTGATTTTGG CTGTCCACAG CGTTCATCAG ATGGGCTATG TACATCGAGA CATCAAGCCC 
GAGAACATCC TCATTGACCG AACGGGACAC ATCAAGCTGG TGGATTTTGG ATCAGCTGCT 
AAAATGAACT CAAATAAGAT GGTAAATGCC AAACTCCCGG TTGGTACTCC AGATTACATG 
GCCCCTGAAG TGTTGACTGT GATGAATGGG GATGGAAAAG GTGCCTATAG CCTAGACTGT 
GATTGGTGGT CAGTGGGAGT TATTGCTTAT GAGATGGTTT ATGGAAGATG CCCATTCACT 
GAAGGAACCT CAGCCAAAAC CTTCAATAAC ATCATGAATT TCCAGCGGTT CTTGAAGTTT 
CCAGATGACC CCAAAGTTAG CAGTGAATTG CTTGATCTAA TCCAGAGTTT GTTGTGTGGC 
CAGAAAGAGA GACTGAAGTT TGAGGGCCTT TGCTGTCATC CTTTCTTCTC CAAAATTGAC 
TGGATTAACA TTCGTAACTC TCCTCCCCCC TTCGTTCCCA CCCTCAAGTC TGATGATGAC 
ACCTCCAATT TTGATGAACC AGAGAAGAAT TCGTGGGTTT CATCCTCTCC GTGCCAGCTG 
AACCCCTCAG GTTTCTCGGG CGAAGAACTG CCGTTTGTGG GGTTTTCGTA TAGCAAGGCA 
CTGGGAATTC TTGGTAGATC TGAGCCTGTT GTATCAAGTC TGGACTCCCC TGCCAAGACT 
AGCTCCATGG AAAAGAAACT CCTTATCAAA AGCAAAGAGC TGCAGGACTC CCAGGACAAG 
TGTCACAAGA TGGAGCAGGA AATGACCCGG TTACATCGGA GAGTGTCAGA GGTGGAGGCT 
GTGCTTAGTC AGAAGGAGGT GGAGCTGAAG GCCTCTGAGA CTCAGAGATC CCTCCTGGAG 
CAGGACCTTG CTACCTACAT CACAGAATGC AGTAGCTTAA AGCGAAGTTT GGAGCAAGCA 
CGGATGGAGG TGTCCCAAGA GGACGACAAA GCACTGCAGC TCCTCCATGA TATCAGAGAG 
CAGAGCCGGA AGCTCCAAGA AATCAAAGAG CAGGAGTACC AGGCTCAAGT GGAAGAAATG 
AGGTTGATGA TGAATCAGTT GGAAGAGGAC CTGGTTTCGG CGAGAAGGCG GAGTGACCTC 
TATGAATCGG AGCTCAGAGA GTCTCGGCTG GCTGCTGAAG AGTTTAAGCG GAAAGCAACA 
GAATGTCAGC ATAAACTGAT GAAGGCTAAA GATCAAGGGA AGCCTGAAGT GGGAGAATAT 
GCCAAACTGG AGAAGATCAA TGCTGAGCAG CAGCTCAAAA TTCAGGAGCT CCAAGAGAAG 
CTAGAAAAGG CTGTGAAAGC TAGCACAGAG GCCACCGAGC TGCTGCAGAA CATCCGTCAG 
GCGAAGGAGC GGGCCGAGCG TGAGCTGGAG AAGCTGCACA ACCGTGAGGA CTCCTCCGAG 
GGCATCAAGA AGAAGCTGGT GGAGGCCGAG GAACGCCGCC ACTCTCTGGA GAACAAGGTA 
AAGAGGCTAG AGACCATGGA GCGTAGAGAA AACAGACTGA AGGATGACAT CCAGACAAAG 
TCCCAACAGA TCCAGCAGAT GGCTGATAAA ATTCTGGAGC TGGAGGAGAA GCACAGAGAA 
GCCCAGGTCT CAGCGCAGCA TCTGGAGGTG CACCTGAAAC AGAAAGAGCA GCACTATGAA 
GAAAAAATTA AAGTGTTGGA CAATCAGATA AAGAAAGACC TAGCAGACAA GGAGACACTG 
GAGAACCTGA TGCAAAGACA CGAAGAGGAG GCCCATGAGA AAGGCAAAAT TCTCAGCGAG 
CAGAAGGCGA TGATCAATGC TATGGATTCC AAGATCAGAT CCCTGGAACA AAGGATAGTG 
GAATTGTCGG AAGCCAATAA ACTTGCGGCC AACAGCAGTC TCTTTACCCA GAGGAACATG 
AAGGCCCAGG AAGAGATGAT TTCAGAACTC AGGCAACAGA AGTTTTACCT GGAGACGCAA 
GCTGGGAAAT TGGAGGCCCA GAACCGGAAG CTGGAGGAGC AGCTGGAAAA AATAAGCCAC 
CAAGACCACA GTGACAAGAA CCGTCTGCTG GAGCTGGAGA CCAGGCTAAG GGAGGTCAGT 
CTAGAACATG AAGAGCAGAA ACTGGAGCTC AAACGCCAGC TCACAGAGCT GCAGCTCTCC 
CTGCAGGAGC GTGAATCCCA GCTGACAGCC CTGCAGGCAG CACGGGCGGC CCTGGAGAGC 
CAGCTCCGCC AGGCCAAGAC GGAGCTGGAA GAGACGACAG CAGAAGCAGA AGAAGAGATC 
CAGGCGCTTA CGGCACATAG AGATGAAATC CAGCGCAAAT TCGATGCCCT TCGTAACAGC 
TGTACTGTGA TCACGGACTT GGAGGAGCAG CTAAACCAGC TGACGGAGGA CAATGCCGAG 
CTCAACAACC AAAACTTCTA CTTGTCTAAA CAACTCGATG AGGCCTCTGG TGCCAACGAC 
GAGATTGTGC AGCTGCGAAG TGAAGTGGAC CATCTCCGCC GCGAGATCAC TGAGAGGGAG 
ATGCAGCTCA CTAGCCAGAA GCAAACGATG GAGGCGCTGA AGACCACGTG CACAATGCTG 
GAGGAGCAGG TCATGGACCT GGAGGCCCTG AACGACGAGC TGCTGGAGAA GGAGCGGCAG 
TGGGAGGCCT GGAGGAGTGT CCTTGGTGAC GAGAAGTCCC AGTTTGAGTG TCGGGTTCGA 
GAGTTGCAGA GGATGCTGGA CACCGAAAAG CAGAGCAGGG CGAGGGCCGA CCAGAGGATC 
ACTGAGTCGC GCCAGGTGGT CGAGCTGGCA GTAAAGGAGC ACAAGGCCGA GATCCTGGCC 
TTGCAGCAGG CTCTCAAGGA GCAGAAGCTG AAGGCCGAGA GCCTCTCCGA CAAGCTCAAT 
GATCTGGAGA AGAAGCACGC CATGCTTGAA ATGAATGCCC GAAGTTTACA ACAGAAGCTG 
GAGACAGAAC GAGAGCTCAA ACAAAGGCTT CTGGAAGAGC AAGCCAAGTT ACAGCAGCAG 
ATGGACCTGC AGAAGAATCA TATTTTCCGT CTGACTCAAG GGCTGCAAGA AGCTCTGGAT 
CGGGCTGATC TGCTGAAGAC AGAAAGGAGT GACCTGGAAT ATCAGCTGGA AAACATTCAG 
GTTCTCTATT CTCATGAGAA GGTGAAGATG GAAGGCACTA TTTCCCAGCA AACCAAACTC 
ATCGATTTTC TGCAAGCCAA AATGGACCAG CCTGCCAAAA AGAAAAAGGG TTTATTTAGT 
CGACGGAAAG AGGACCCTGC TTTGCCCACA CAGGTTCCTC TGCAGTACAA TGAGCTGAAG 
GTGGCTCTGG AGAAGGAGAA AGCTCGCTGC GCGGAGCTCG AGGAAGCCCT GCAGAAGACC 
CGCATCGAGC TCCGCTCCGC CCGGGAGGAA GCTGCCCACC GGAAAGCTGC AGACCACCCA 
CACCCATCTA CGCCGGCTAC CGCCAGGCAG CAGATCGCCA TGTCCGCCAT TGTGCGGTCA 
CCCGAGCACC AGCCCAGCGC CCTGAGCCTG CTCGCCCCGC CGTCCAGCCG CAGGAAGGAG 
TCTTCCACTC CAGAGGAATT CAGCCGGCGT CTTAAGGAGC GCATGCACCA CAACATCCCT 
CACCGGTTCA ACGTGGGACT GAACATGCGA GCCACGAAGT GCGCCGTGTG TCTGGATACC 
GTGCACTTCG GGCGCCAGGC ATCCAAGTGT CTCGAATGTC AGGTCATGTG CCACCCCAAG 
TGCTCCACAT GCTTGCCAGC CACCTGTGGC CTGCCAGCCG AATACGCCAC ACACTTCACC 
GAGGCCTTCT GCCGCGACAA AATGAACTCC CCGGGTCTGC AGTCCAAGGA GCCCAGCAGC 
AGCCTGCACC TGGAAGGGTG GATGAAGGTG CCCAGGAATA ACAAGCGAGG ACAGCAAGGC 
TGGGACAGGA AGTACATCGT TCTGGAGGGC TCCAAAGTCC TCATTTATGA CAACGAAGCC 
AGAGAAGCTG GACAGAGGCC GGTGGAAGAA TTTGAGCTGT GCCTTCCCGA CGGGGACGTG 
TCTATTCATG GCGCCGTTGG TGCTTCTGAA CTTGCAAACA CAGCCAAAGC AGATGTCCCC 
TATGTCCTGA AGATGGAGTC TCACCCCCAT ACCACCTGCT GGCCTGGGAG GACCCTCTAC 
TTGCTGGCTC CCAGCTTTCC CGACAAGCAG CGCTGGGTCA CCGCCTTAGA GTCAGTTGTG 
GCAGGTGGGA GAGTGTCTAG GGAAAAGGCG GAAGCCGACG CTGGCCGTGA CTGTACTTCC 
TACGAGCCTC TGCCCGCGTG GGCTCAGAAA TTGCTCGGAA ACTCCCTGTT GAAACTGGAA 
GGTGATGACC GTCTGGACAT GAACTGCACG CTGCCCTTCA GCGACCAGGT GGTGCTGGTG 
GGCACCGAGG AAGGGCTGTA TGCGCTGAAT GTCTTGAAGA ACTCCCTCAC CCACGTCCCA 
GGAATCGGAG CAGTCTTCCA GATTTACATC ATCAAGGACC TGGAGAAGCT CCTCATGATC 
GCAGGAGAGG AGCGGGCCCT GTGTCTCGTG GATGTGAAGA AGGTGAAGCA GTCCCTCGCG 
CAGTCTCACC TCCCCGCCCA GCCAGACATC TCGCCCAACA TCTTTGAAGC GGTCAAGGGC 
TGCCACTTGT TTGCTGCTGG CAAGATTGAG AGCGGGCTCT GCATCTGTGC AGCCATGCCC 
AGCAAAGTTG TCATTCTCCG CTACAATGAA AACCTCAGCA AGTACTGCAT TCGCAAAGAG 
ATCGAGACCT CGGAGCCCTG CAGCTGTATC CACTTCACCA ACTACAGTAT CCTCATCGGA 
ACCAATAAAT TCTACGAAAT CGACATGAAG CAGTACACGC TAGAGGAATT CCTGGATAAG 
AACGACCATT CCTTGGCGCC TGCCGTGTTT GCCTCCTCTT CCAACAGCTT CCCCGTGTCC 
ATCATGCAGG TGAACGGCGC GGGGCAGCGG GAGGAGTTCC TGCTCTGTTT CCACGAATTC 
GGGGTGTTCG TGGATTCTTA TGGAAGACGC AGCCGCACAG ATGATCTCAA GTGGAGTCGC 
TTACCTCTGG CCTTTGCCTA CAGAGAACCC TATCTGTTTG TGACTCACTT CAACTCACTC 
GAAGTAATTG AGATCCAGGC CCGCTCCTCT CTGGGGACCC CCGCCCGAGC ATATTTGGAA 
ATCCCCAACC CACGCTACCT GGGCCCTGCA ATCTCCTCAG GAGCCATCTA CCTGGCGTCC 
TCATATCAGG ATAAGTTAAG GGTCATCTGC TGCAAAGGAA ACCTGGTGAA GGAGACCGGC 
ACCGACCACC ACCGTGGCCC ATCCACCTCC CGCAGCCCCA ACAAGCGAGG CCCGCCGACG 
TACAACGAGC ACATCACCAA GCGCGTGGCC TCAAGCCCGG CGCCTCCCGA AGGCCCCAGC 
CACCCTCGAG AGCCAAGCAC ACCCCACCGC TACCGCGAGG GGCGGACCGA GCTGCGCAGG 
GACAAGTCTC CGGGCCGCCC CCTGGAGCGC GAGAAGTCCC CCGGCCGGAT GCTGAGCACG 
CGGAGGGAGC GCTCCCCCGG GCGGCTGTTT GAGGACAGCA GCAGGGGCCG GCTGCCCGTG 
GGGGCCGTGA GGACCCCGCT GTCCCAGGTC AACAAGGTCT GGGACCAGTC TTCAGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024833507.1
CDS	238..6117
Translation	MKVMKKKALLAQEQVSYFEEEQNILSQSTSPWIPQLQYAFQDKNNLYLVMEYQPGGDLLSLLNRYEDQLDENMIQFYLAELILAVHSVHQMGYVHRDIKPENILIDRTGHIKLVDFGSAAKMNSNKMVNAKLPVGTPDYMAPEVLTVMNGDGKGAYSLDCDWWSVGVIAYEMVYGRCPFTEGTSAKTFNNIMNFQRFLKFPDDPKVSSELLDLIQSLLCGQKERLKFEGLCCHPFFSKIDWINIRNSPPPFVPTLKSDDDTSNFDEPEKNSWVSSSPCQLNPSGFSGEELPFVGFSYSKALGILGRSEPVVSSLDSPAKTSSMEKKLLIKSKELQDSQDKCHKMEQEMTRLHRRVSEVEAVLSQKEVELKASETQRSLLEQDLATYITECSSLKRSLEQARMEVSQEDDKALQLLHDIREQSRKLQEIKEQEYQAQVEEMRLMMNQLEEDLVSARRRSDLYESELRESRLAAEEFKRKATECQHKLMKAKDQGKPEVGEYAKLEKINAEQQLKIQELQEKLEKAVKASTEATELLQNIRQAKERAERELEKLHNREDSSEGIKKKLVEAEERRHSLENKVKRLETMERRENRLKDDIQTKSQQIQQMADKILELEEKHREAQVSAQHLEVHLKQKEQHYEEKIKVLDNQIKKDLADKETLENLMQRHEEEAHEKGKILSEQKAMINAMDSKIRSLEQRIVELSEANKLAANSSLFTQRNMKAQEEMISELRQQKFYLETQAGKLEAQNRKLEEQLEKISHQDHSDKNRLLELETRLREVSLEHEEQKLELKRQLTELQLSLQERESQLTALQAARAALESQLRQAKTELEETTAEAEEEIQALTAHRDEIQRKFDALRNSCTVITDLEEQLNQLTEDNAELNNQNFYLSKQLDEASGANDEIVQLRSEVDHLRREITEREMQLTSQKQTMEALKTTCTMLEEQVMDLEALNDELLEKERQWEAWRSVLGDEKSQFECRVRELQRMLDTEKQSRARADQRITESRQVVELAVKEHKAEILALQQALKEQKLKAESLSDKLNDLEKKHAMLEMNARSLQQKLETERELKQRLLEEQAKLQQQMDLQKNHIFRLTQGLQEALDRADLLKTERSDLEYQLENIQVLYSHEKVKMEGTISQQTKLIDFLQAKMDQPAKKKKGLFSRRKEDPALPTQVPLQYNELKVALEKEKARCAELEEALQKTRIELRSAREEAAHRKAADHPHPSTPATARQQIAMSAIVRSPEHQPSALSLLAPPSSRRKESSTPEEFSRRLKERMHHNIPHRFNVGLNMRATKCAVCLDTVHFGRQASKCLECQVMCHPKCSTCLPATCGLPAEYATHFTEAFCRDKMNSPGLQSKEPSSSLHLEGWMKVPRNNKRGQQGWDRKYIVLEGSKVLIYDNEAREAGQRPVEEFELCLPDGDVSIHGAVGASELANTAKADVPYVLKMESHPHTTCWPGRTLYLLAPSFPDKQRWVTALESVVAGGRVSREKAEADAGRDCTSYEPLPAWAQKLLGNSLLKLEGDDRLDMNCTLPFSDQVVLVGTEEGLYALNVLKNSLTHVPGIGAVFQIYIIKDLEKLLMIAGEERALCLVDVKKVKQSLAQSHLPAQPDISPNIFEAVKGCHLFAAGKIESGLCICAAMPSKVVILRYNENLSKYCIRKEIETSEPCSCIHFTNYSILIGTNKFYEIDMKQYTLEEFLDKNDHSLAPAVFASSSNSFPVSIMQVNGAGQREEFLLCFHEFGVFVDSYGRRSRTDDLKWSRLPLAFAYREPYLFVTHFNSLEVIEIQARSSLGTPARAYLEIPNPRYLGPAISSGAIYLASSYQDKLRVICCKGNLVKETGTDHHRGPSTSRSPNKRGPPTYNEHITKRVASSPAPPEGPSHPREPSTPHRYREGRTELRRDKSPGRPLEREKSPGRMLSTRRERSPGRLFEDSSRGRLPVGAVRTPLSQVNKVWDQSSV

Target ORF information:

RefSeq Version	XM_024977739.1
Organism	Bos taurus(cattle)
Definition	Bos taurus citron rho-interacting serine/threonine kinase (CIT), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024977739.1

ORF Insert Sequence:

ATGAAAGTCA TGAAGAAGAA GGCCTTGTTG GCCCAGGAGC AGGTCTCATA TTTTGAGGAA 
GAACAGAACA TATTATCTCA GAGTACAAGT CCTTGGATCC CCCAGTTGCA GTATGCCTTT 
CAGGACAAAA ATAACCTTTA TCTGGTCATG GAATATCAGC CTGGAGGGGA TTTGCTGTCA 
CTTTTGAATA GATATGAGGA CCAATTAGAT GAAAATATGA TTCAGTTTTA CCTAGCTGAA 
CTGATTTTGG CTGTCCACAG CGTTCATCAG ATGGGCTATG TACATCGAGA CATCAAGCCC 
GAGAACATCC TCATTGACCG AACGGGACAC ATCAAGCTGG TGGATTTTGG ATCAGCTGCT 
AAAATGAACT CAAATAAGAT GGTAAATGCC AAACTCCCGG TTGGTACTCC AGATTACATG 
GCCCCTGAAG TGTTGACTGT GATGAATGGG GATGGAAAAG GTGCCTATAG CCTAGACTGT 
GATTGGTGGT CAGTGGGAGT TATTGCTTAT GAGATGGTTT ATGGAAGATG CCCATTCACT 
GAAGGAACCT CAGCCAAAAC CTTCAATAAC ATCATGAATT TCCAGCGGTT CTTGAAGTTT 
CCAGATGACC CCAAAGTTAG CAGTGAATTG CTTGATCTAA TCCAGAGTTT GTTGTGTGGC 
CAGAAAGAGA GACTGAAGTT TGAGGGCCTT TGCTGTCATC CTTTCTTCTC CAAAATTGAC 
TGGATTAACA TTCGTAACTC TCCTCCCCCC TTCGTTCCCA CCCTCAAGTC TGATGATGAC 
ACCTCCAATT TTGATGAACC AGAGAAGAAT TCGTGGGTTT CATCCTCTCC GTGCCAGCTG 
AACCCCTCAG GTTTCTCGGG CGAAGAACTG CCGTTTGTGG GGTTTTCGTA TAGCAAGGCA 
CTGGGAATTC TTGGTAGATC TGAGCCTGTT GTATCAAGTC TGGACTCCCC TGCCAAGACT 
AGCTCCATGG AAAAGAAACT CCTTATCAAA AGCAAAGAGC TGCAGGACTC CCAGGACAAG 
TGTCACAAGA TGGAGCAGGA AATGACCCGG TTACATCGGA GAGTGTCAGA GGTGGAGGCT 
GTGCTTAGTC AGAAGGAGGT GGAGCTGAAG GCCTCTGAGA CTCAGAGATC CCTCCTGGAG 
CAGGACCTTG CTACCTACAT CACAGAATGC AGTAGCTTAA AGCGAAGTTT GGAGCAAGCA 
CGGATGGAGG TGTCCCAAGA GGACGACAAA GCACTGCAGC TCCTCCATGA TATCAGAGAG 
CAGAGCCGGA AGCTCCAAGA AATCAAAGAG CAGGAGTACC AGGCTCAAGT GGAAGAAATG 
AGGTTGATGA TGAATCAGTT GGAAGAGGAC CTGGTTTCGG CGAGAAGGCG GAGTGACCTC 
TATGAATCGG AGCTCAGAGA GTCTCGGCTG GCTGCTGAAG AGTTTAAGCG GAAAGCAACA 
GAATGTCAGC ATAAACTGAT GAAGGCTAAA GATCAAGGGA AGCCTGAAGT GGGAGAATAT 
GCCAAACTGG AGAAGATCAA TGCTGAGCAG CAGCTCAAAA TTCAGGAGCT CCAAGAGAAG 
CTAGAAAAGG CTGTGAAAGC TAGCACAGAG GCCACCGAGC TGCTGCAGAA CATCCGTCAG 
GCGAAGGAGC GGGCCGAGCG TGAGCTGGAG AAGCTGCACA ACCGTGAGGA CTCCTCCGAG 
GGCATCAAGA AGAAGCTGGT GGAGGCCGAG GAACGCCGCC ACTCTCTGGA GAACAAGGTA 
AAGAGGCTAG AGACCATGGA GCGTAGAGAA AACAGACTGA AGGATGACAT CCAGACAAAG 
TCCCAACAGA TCCAGCAGAT GGCTGATAAA ATTCTGGAGC TGGAGGAGAA GCACAGAGAA 
GCCCAGGTCT CAGCGCAGCA TCTGGAGGTG CACCTGAAAC AGAAAGAGCA GCACTATGAA 
GAAAAAATTA AAGTGTTGGA CAATCAGATA AAGAAAGACC TAGCAGACAA GGAGACACTG 
GAGAACCTGA TGCAAAGACA CGAAGAGGAG GCCCATGAGA AAGGCAAAAT TCTCAGCGAG 
CAGAAGGCGA TGATCAATGC TATGGATTCC AAGATCAGAT CCCTGGAACA AAGGATAGTG 
GAATTGTCGG AAGCCAATAA ACTTGCGGCC AACAGCAGTC TCTTTACCCA GAGGAACATG 
AAGGCCCAGG AAGAGATGAT TTCAGAACTC AGGCAACAGA AGTTTTACCT GGAGACGCAA 
GCTGGGAAAT TGGAGGCCCA GAACCGGAAG CTGGAGGAGC AGCTGGAAAA AATAAGCCAC 
CAAGACCACA GTGACAAGAA CCGTCTGCTG GAGCTGGAGA CCAGGCTAAG GGAGGTCAGT 
CTAGAACATG AAGAGCAGAA ACTGGAGCTC AAACGCCAGC TCACAGAGCT GCAGCTCTCC 
CTGCAGGAGC GTGAATCCCA GCTGACAGCC CTGCAGGCAG CACGGGCGGC CCTGGAGAGC 
CAGCTCCGCC AGGCCAAGAC GGAGCTGGAA GAGACGACAG CAGAAGCAGA AGAAGAGATC 
CAGGCGCTTA CGGCACATAG AGATGAAATC CAGCGCAAAT TCGATGCCCT TCGTAACAGC 
TGTACTGTGA TCACGGACTT GGAGGAGCAG CTAAACCAGC TGACGGAGGA CAATGCCGAG 
CTCAACAACC AAAACTTCTA CTTGTCTAAA CAACTCGATG AGGCCTCTGG TGCCAACGAC 
GAGATTGTGC AGCTGCGAAG TGAAGTGGAC CATCTCCGCC GCGAGATCAC TGAGAGGGAG 
ATGCAGCTCA CTAGCCAGAA GCAAACGATG GAGGCGCTGA AGACCACGTG CACAATGCTG 
GAGGAGCAGG TCATGGACCT GGAGGCCCTG AACGACGAGC TGCTGGAGAA GGAGCGGCAG 
TGGGAGGCCT GGAGGAGTGT CCTTGGTGAC GAGAAGTCCC AGTTTGAGTG TCGGGTTCGA 
GAGTTGCAGA GGATGCTGGA CACCGAAAAG CAGAGCAGGG CGAGGGCCGA CCAGAGGATC 
ACTGAGTCGC GCCAGGTGGT CGAGCTGGCA GTAAAGGAGC ACAAGGCCGA GATCCTGGCC 
TTGCAGCAGG CTCTCAAGGA GCAGAAGCTG AAGGCCGAGA GCCTCTCCGA CAAGCTCAAT 
GATCTGGAGA AGAAGCACGC CATGCTTGAA ATGAATGCCC GAAGTTTACA ACAGAAGCTG 
GAGACAGAAC GAGAGCTCAA ACAAAGGCTT CTGGAAGAGC AAGCCAAGTT ACAGCAGCAG 
ATGGACCTGC AGAAGAATCA TATTTTCCGT CTGACTCAAG GGCTGCAAGA AGCTCTGGAT 
CGGGCTGATC TGCTGAAGAC AGAAAGGAGT GACCTGGAAT ATCAGCTGGA AAACATTCAG 
GTTCTCTATT CTCATGAGAA GGTGAAGATG GAAGGCACTA TTTCCCAGCA AACCAAACTC 
ATCGATTTTC TGCAAGCCAA AATGGACCAG CCTGCCAAAA AGAAAAAGGG TTTATTTAGT 
CGACGGAAAG AGGACCCTGC TTTGCCCACA CAGGTTCCTC TGCAGTACAA TGAGCTGAAG 
GTGGCTCTGG AGAAGGAGAA AGCTCGCTGC GCGGAGCTCG AGGAAGCCCT GCAGAAGACC 
CGCATCGAGC TCCGCTCCGC CCGGGAGGAA GCTGCCCACC GGAAAGCTGC AGACCACCCA 
CACCCATCTA CGCCGGCTAC CGCCAGGCAG CAGATCGCCA TGTCCGCCAT TGTGCGGTCA 
CCCGAGCACC AGCCCAGCGC CCTGAGCCTG CTCGCCCCGC CGTCCAGCCG CAGGAAGGAG 
TCTTCCACTC CAGAGGAATT CAGCCGGCGT CTTAAGGAGC GCATGCACCA CAACATCCCT 
CACCGGTTCA ACGTGGGACT GAACATGCGA GCCACGAAGT GCGCCGTGTG TCTGGATACC 
GTGCACTTCG GGCGCCAGGC ATCCAAGTGT CTCGAATGTC AGGTCATGTG CCACCCCAAG 
TGCTCCACAT GCTTGCCAGC CACCTGTGGC CTGCCAGCCG AATACGCCAC ACACTTCACC 
GAGGCCTTCT GCCGCGACAA AATGAACTCC CCGGGTCTGC AGTCCAAGGA GCCCAGCAGC 
AGCCTGCACC TGGAAGGGTG GATGAAGGTG CCCAGGAATA ACAAGCGAGG ACAGCAAGGC 
TGGGACAGGA AGTACATCGT TCTGGAGGGC TCCAAAGTCC TCATTTATGA CAACGAAGCC 
AGAGAAGCTG GACAGAGGCC GGTGGAAGAA TTTGAGCTGT GCCTTCCCGA CGGGGACGTG 
TCTATTCATG GCGCCGTTGG TGCTTCTGAA CTTGCAAACA CAGCCAAAGC AGATGTCCCC 
TATGTCCTGA AGATGGAGTC TCACCCCCAT ACCACCTGCT GGCCTGGGAG GACCCTCTAC 
TTGCTGGCTC CCAGCTTTCC CGACAAGCAG CGCTGGGTCA CCGCCTTAGA GTCAGTTGTG 
GCAGGTGGGA GAGTGTCTAG GGAAAAGGCG GAAGCCGACG CTGGCCGTGA CTGTACTTCC 
TACGAGCCTC TGCCCGCGTG GGCTCAGAAA TTGCTCGGAA ACTCCCTGTT GAAACTGGAA 
GGTGATGACC GTCTGGACAT GAACTGCACG CTGCCCTTCA GCGACCAGGT GGTGCTGGTG 
GGCACCGAGG AAGGGCTGTA TGCGCTGAAT GTCTTGAAGA ACTCCCTCAC CCACGTCCCA 
GGAATCGGAG CAGTCTTCCA GATTTACATC ATCAAGGACC TGGAGAAGCT CCTCATGATC 
GCAGGAGAGG AGCGGGCCCT GTGTCTCGTG GATGTGAAGA AGGTGAAGCA GTCCCTCGCG 
CAGTCTCACC TCCCCGCCCA GCCAGACATC TCGCCCAACA TCTTTGAAGC GGTCAAGGGC 
TGCCACTTGT TTGCTGCTGG CAAGATTGAG AGCGGGCTCT GCATCTGTGC AGCCATGCCC 
AGCAAAGTTG TCATTCTCCG CTACAATGAA AACCTCAGCA AGTACTGCAT TCGCAAAGAG 
ATCGAGACCT CGGAGCCCTG CAGCTGTATC CACTTCACCA ACTACAGTAT CCTCATCGGA 
ACCAATAAAT TCTACGAAAT CGACATGAAG CAGTACACGC TAGAGGAATT CCTGGATAAG 
AACGACCATT CCTTGGCGCC TGCCGTGTTT GCCTCCTCTT CCAACAGCTT CCCCGTGTCC 
ATCATGCAGG TGAACGGCGC GGGGCAGCGG GAGGAGTTCC TGCTCTGTTT CCACGAATTC 
GGGGTGTTCG TGGATTCTTA TGGAAGACGC AGCCGCACAG ATGATCTCAA GTGGAGTCGC 
TTACCTCTGG CCTTTGCCTA CAGAGAACCC TATCTGTTTG TGACTCACTT CAACTCACTC 
GAAGTAATTG AGATCCAGGC CCGCTCCTCT CTGGGGACCC CCGCCCGAGC ATATTTGGAA 
ATCCCCAACC CACGCTACCT GGGCCCTGCA ATCTCCTCAG GAGCCATCTA CCTGGCGTCC 
TCATATCAGG ATAAGTTAAG GGTCATCTGC TGCAAAGGAA ACCTGGTGAA GGAGACCGGC 
ACCGACCACC ACCGTGGCCC ATCCACCTCC CGCAGCCCCA ACAAGCGAGG CCCGCCGACG 
TACAACGAGC ACATCACCAA GCGCGTGGCC TCAAGCCCGG CGCCTCCCGA AGGCCCCAGC 
CACCCTCGAG AGCCAAGCAC ACCCCACCGC TACCGCGAGG GGCGGACCGA GCTGCGCAGG 
GACAAGTCTC CGGGCCGCCC CCTGGAGCGC GAGAAGTCCC CCGGCCGGAT GCTGAGCACG 
CGGAGGGAGC GCTCCCCCGG GCGGCTGTTT GAGGACAGCA GCAGGGGCCG GCTGCCCGTG 
GGGGCCGTGA GGACCCCGCT GTCCCAGGTC AACAAGGTCT GGGACCAGTC TTCAGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CIT ( XM_024977740.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024833508.1 Bos taurus citron rho-interacting serine/threonine kinase (CIT), transcript variant X8, mRNA.

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CloneID	OBa224628
Clone ID Related Accession (Same CDS sequence)	XM_024977740.1
Accession Version	XM_024977740.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5517bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	citron Rho-interacting kinase isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACTCAA ATAAGATGGT AAATGCCAAA CTCCCGGTTG GTACTCCAGA TTACATGGCC 
CCTGAAGTGT TGACTGTGAT GAATGGGGAT GGAAAAGGTG CCTATAGCCT AGACTGTGAT 
TGGTGGTCAG TGGGAGTTAT TGCTTATGAG ATGGTTTATG GAAGATGCCC ATTCACTGAA 
GGAACCTCAG CCAAAACCTT CAATAACATC ATGAATTTCC AGCGGTTCTT GAAGTTTCCA 
GATGACCCCA AAGTTAGCAG TGAATTGCTT GATCTAATCC AGAGTTTGTT GTGTGGCCAG 
AAAGAGAGAC TGAAGTTTGA GGGCCTTTGC TGTCATCCTT TCTTCTCCAA AATTGACTGG 
ATTAACATTC GTAACTCTCC TCCCCCCTTC GTTCCCACCC TCAAGTCTGA TGATGACACC 
TCCAATTTTG ATGAACCAGA GAAGAATTCG TGGGTTTCAT CCTCTCCGTG CCAGCTGAAC 
CCCTCAGGTT TCTCGGGCGA AGAACTGCCG TTTGTGGGGT TTTCGTATAG CAAGGCACTG 
GGAATTCTTG GTAGATCTGA GCCTGTTGTA TCAAGTCTGG ACTCCCCTGC CAAGACTAGC 
TCCATGGAAA AGAAACTCCT TATCAAAAGC AAAGAGCTGC AGGACTCCCA GGACAAGTGT 
CACAAGATGG AGCAGGAAAT GACCCGGTTA CATCGGAGAG TGTCAGAGGT GGAGGCTGTG 
CTTAGTCAGA AGGAGGTGGA GCTGAAGGCC TCTGAGACTC AGAGATCCCT CCTGGAGCAG 
GACCTTGCTA CCTACATCAC AGAATGCAGT AGCTTAAAGC GAAGTTTGGA GCAAGCACGG 
ATGGAGGTGT CCCAAGAGGA CGACAAAGCA CTGCAGCTCC TCCATGATAT CAGAGAGCAG 
AGCCGGAAGC TCCAAGAAAT CAAAGAGCAG GAGTACCAGG CTCAAGTGGA AGAAATGAGG 
TTGATGATGA ATCAGTTGGA AGAGGACCTG GTTTCGGCGA GAAGGCGGAG TGACCTCTAT 
GAATCGGAGC TCAGAGAGTC TCGGCTGGCT GCTGAAGAGT TTAAGCGGAA AGCAACAGAA 
TGTCAGCATA AACTGATGAA GGCTAAAGAT CAAGGGAAGC CTGAAGTGGG AGAATATGCC 
AAACTGGAGA AGATCAATGC TGAGCAGCAG CTCAAAATTC AGGAGCTCCA AGAGAAGCTA 
GAAAAGGCTG TGAAAGCTAG CACAGAGGCC ACCGAGCTGC TGCAGAACAT CCGTCAGGCG 
AAGGAGCGGG CCGAGCGTGA GCTGGAGAAG CTGCACAACC GTGAGGACTC CTCCGAGGGC 
ATCAAGAAGA AGCTGGTGGA GGCCGAGGAA CGCCGCCACT CTCTGGAGAA CAAGGTAAAG 
AGGCTAGAGA CCATGGAGCG TAGAGAAAAC AGACTGAAGG ATGACATCCA GACAAAGTCC 
CAACAGATCC AGCAGATGGC TGATAAAATT CTGGAGCTGG AGGAGAAGCA CAGAGAAGCC 
CAGGTCTCAG CGCAGCATCT GGAGGTGCAC CTGAAACAGA AAGAGCAGCA CTATGAAGAA 
AAAATTAAAG TGTTGGACAA TCAGATAAAG AAAGACCTAG CAGACAAGGA GACACTGGAG 
AACCTGATGC AAAGACACGA AGAGGAGGCC CATGAGAAAG GCAAAATTCT CAGCGAGCAG 
AAGGCGATGA TCAATGCTAT GGATTCCAAG ATCAGATCCC TGGAACAAAG GATAGTGGAA 
TTGTCGGAAG CCAATAAACT TGCGGCCAAC AGCAGTCTCT TTACCCAGAG GAACATGAAG 
GCCCAGGAAG AGATGATTTC AGAACTCAGG CAACAGAAGT TTTACCTGGA GACGCAAGCT 
GGGAAATTGG AGGCCCAGAA CCGGAAGCTG GAGGAGCAGC TGGAAAAAAT AAGCCACCAA 
GACCACAGTG ACAAGAACCG TCTGCTGGAG CTGGAGACCA GGCTAAGGGA GGTCAGTCTA 
GAACATGAAG AGCAGAAACT GGAGCTCAAA CGCCAGCTCA CAGAGCTGCA GCTCTCCCTG 
CAGGAGCGTG AATCCCAGCT GACAGCCCTG CAGGCAGCAC GGGCGGCCCT GGAGAGCCAG 
CTCCGCCAGG CCAAGACGGA GCTGGAAGAG ACGACAGCAG AAGCAGAAGA AGAGATCCAG 
GCGCTTACGG CACATAGAGA TGAAATCCAG CGCAAATTCG ATGCCCTTCG TAACAGCTGT 
ACTGTGATCA CGGACTTGGA GGAGCAGCTA AACCAGCTGA CGGAGGACAA TGCCGAGCTC 
AACAACCAAA ACTTCTACTT GTCTAAACAA CTCGATGAGG CCTCTGGTGC CAACGACGAG 
ATTGTGCAGC TGCGAAGTGA AGTGGACCAT CTCCGCCGCG AGATCACTGA GAGGGAGATG 
CAGCTCACTA GCCAGAAGCA AACGATGGAG GCGCTGAAGA CCACGTGCAC AATGCTGGAG 
GAGCAGGTCA TGGACCTGGA GGCCCTGAAC GACGAGCTGC TGGAGAAGGA GCGGCAGTGG 
GAGGCCTGGA GGAGTGTCCT TGGTGACGAG AAGTCCCAGT TTGAGTGTCG GGTTCGAGAG 
TTGCAGAGGA TGCTGGACAC CGAAAAGCAG AGCAGGGCGA GGGCCGACCA GAGGATCACT 
GAGTCGCGCC AGGTGGTCGA GCTGGCAGTA AAGGAGCACA AGGCCGAGAT CCTGGCCTTG 
CAGCAGGCTC TCAAGGAGCA GAAGCTGAAG GCCGAGAGCC TCTCCGACAA GCTCAATGAT 
CTGGAGAAGA AGCACGCCAT GCTTGAAATG AATGCCCGAA GTTTACAACA GAAGCTGGAG 
ACAGAACGAG AGCTCAAACA AAGGCTTCTG GAAGAGCAAG CCAAGTTACA GCAGCAGATG 
GACCTGCAGA AGAATCATAT TTTCCGTCTG ACTCAAGGGC TGCAAGAAGC TCTGGATCGG 
GCTGATCTGC TGAAGACAGA AAGGAGTGAC CTGGAATATC AGCTGGAAAA CATTCAGGTT 
CTCTATTCTC ATGAGAAGGT GAAGATGGAA GGCACTATTT CCCAGCAAAC CAAACTCATC 
GATTTTCTGC AAGCCAAAAT GGACCAGCCT GCCAAAAAGA AAAAGGGTTT ATTTAGTCGA 
CGGAAAGAGG ACCCTGCTTT GCCCACACAG GTTCCTCTGC AGTACAATGA GCTGAAGGTG 
GCTCTGGAGA AGGAGAAAGC TCGCTGCGCG GAGCTCGAGG AAGCCCTGCA GAAGACCCGC 
ATCGAGCTCC GCTCCGCCCG GGAGGAAGCT GCCCACCGGA AAGCTGCAGA CCACCCACAC 
CCATCTACGC CGGCTACCGC CAGGCAGCAG ATCGCCATGT CCGCCATTGT GCGGTCACCC 
GAGCACCAGC CCAGCGCCCT GAGCCTGCTC GCCCCGCCGT CCAGCCGCAG GAAGGAGTCT 
TCCACTCCAG AGGAATTCAG CCGGCGTCTT AAGGAGCGCA TGCACCACAA CATCCCTCAC 
CGGTTCAACG TGGGACTGAA CATGCGAGCC ACGAAGTGCG CCGTGTGTCT GGATACCGTG 
CACTTCGGGC GCCAGGCATC CAAGTGTCTC GAATGTCAGG TCATGTGCCA CCCCAAGTGC 
TCCACATGCT TGCCAGCCAC CTGTGGCCTG CCAGCCGAAT ACGCCACACA CTTCACCGAG 
GCCTTCTGCC GCGACAAAAT GAACTCCCCG GGTCTGCAGT CCAAGGAGCC CAGCAGCAGC 
CTGCACCTGG AAGGGTGGAT GAAGGTGCCC AGGAATAACA AGCGAGGACA GCAAGGCTGG 
GACAGGAAGT ACATCGTTCT GGAGGGCTCC AAAGTCCTCA TTTATGACAA CGAAGCCAGA 
GAAGCTGGAC AGAGGCCGGT GGAAGAATTT GAGCTGTGCC TTCCCGACGG GGACGTGTCT 
ATTCATGGCG CCGTTGGTGC TTCTGAACTT GCAAACACAG CCAAAGCAGA TGTCCCCTAT 
GTCCTGAAGA TGGAGTCTCA CCCCCATACC ACCTGCTGGC CTGGGAGGAC CCTCTACTTG 
CTGGCTCCCA GCTTTCCCGA CAAGCAGCGC TGGGTCACCG CCTTAGAGTC AGTTGTGGCA 
GGTGGGAGAG TGTCTAGGGA AAAGGCGGAA GCCGACGCTG GCCGTGACTG TACTTCCTAC 
GAGCCTCTGC CCGCGTGGGC TCAGAAATTG CTCGGAAACT CCCTGTTGAA ACTGGAAGGT 
GATGACCGTC TGGACATGAA CTGCACGCTG CCCTTCAGCG ACCAGGTGGT GCTGGTGGGC 
ACCGAGGAAG GGCTGTATGC GCTGAATGTC TTGAAGAACT CCCTCACCCA CGTCCCAGGA 
ATCGGAGCAG TCTTCCAGAT TTACATCATC AAGGACCTGG AGAAGCTCCT CATGATCGCA 
GGAGAGGAGC GGGCCCTGTG TCTCGTGGAT GTGAAGAAGG TGAAGCAGTC CCTCGCGCAG 
TCTCACCTCC CCGCCCAGCC AGACATCTCG CCCAACATCT TTGAAGCGGT CAAGGGCTGC 
CACTTGTTTG CTGCTGGCAA GATTGAGAGC GGGCTCTGCA TCTGTGCAGC CATGCCCAGC 
AAAGTTGTCA TTCTCCGCTA CAATGAAAAC CTCAGCAAGT ACTGCATTCG CAAAGAGATC 
GAGACCTCGG AGCCCTGCAG CTGTATCCAC TTCACCAACT ACAGTATCCT CATCGGAACC 
AATAAATTCT ACGAAATCGA CATGAAGCAG TACACGCTAG AGGAATTCCT GGATAAGAAC 
GACCATTCCT TGGCGCCTGC CGTGTTTGCC TCCTCTTCCA ACAGCTTCCC CGTGTCCATC 
ATGCAGGTGA ACGGCGCGGG GCAGCGGGAG GAGTTCCTGC TCTGTTTCCA CGAATTCGGG 
GTGTTCGTGG ATTCTTATGG AAGACGCAGC CGCACAGATG ATCTCAAGTG GAGTCGCTTA 
CCTCTGGCCT TTGCCTACAG AGAACCCTAT CTGTTTGTGA CTCACTTCAA CTCACTCGAA 
GTAATTGAGA TCCAGGCCCG CTCCTCTCTG GGGACCCCCG CCCGAGCATA TTTGGAAATC 
CCCAACCCAC GCTACCTGGG CCCTGCAATC TCCTCAGGAG CCATCTACCT GGCGTCCTCA 
TATCAGGATA AGTTAAGGGT CATCTGCTGC AAAGGAAACC TGGTGAAGGA GACCGGCACC 
GACCACCACC GTGGCCCATC CACCTCCCGC AGCCCCAACA AGCGAGGCCC GCCGACGTAC 
AACGAGCACA TCACCAAGCG CGTGGCCTCA AGCCCGGCGC CTCCCGAAGG CCCCAGCCAC 
CCTCGAGAGC CAAGCACACC CCACCGCTAC CGCGAGGGGC GGACCGAGCT GCGCAGGGAC 
AAGTCTCCGG GCCGCCCCCT GGAGCGCGAG AAGTCCCCCG GCCGGATGCT GAGCACGCGG 
AGGGAGCGCT CCCCCGGGCG GCTGTTTGAG GACAGCAGCA GGGGCCGGCT GCCCGTGGGG 
GCCGTGAGGA CCCCGCTGTC CCAGGTCAAC AAGGTCTGGG ACCAGTCTTC AGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024833508.1
CDS	177..5693
Translation	MNSNKMVNAKLPVGTPDYMAPEVLTVMNGDGKGAYSLDCDWWSVGVIAYEMVYGRCPFTEGTSAKTFNNIMNFQRFLKFPDDPKVSSELLDLIQSLLCGQKERLKFEGLCCHPFFSKIDWINIRNSPPPFVPTLKSDDDTSNFDEPEKNSWVSSSPCQLNPSGFSGEELPFVGFSYSKALGILGRSEPVVSSLDSPAKTSSMEKKLLIKSKELQDSQDKCHKMEQEMTRLHRRVSEVEAVLSQKEVELKASETQRSLLEQDLATYITECSSLKRSLEQARMEVSQEDDKALQLLHDIREQSRKLQEIKEQEYQAQVEEMRLMMNQLEEDLVSARRRSDLYESELRESRLAAEEFKRKATECQHKLMKAKDQGKPEVGEYAKLEKINAEQQLKIQELQEKLEKAVKASTEATELLQNIRQAKERAERELEKLHNREDSSEGIKKKLVEAEERRHSLENKVKRLETMERRENRLKDDIQTKSQQIQQMADKILELEEKHREAQVSAQHLEVHLKQKEQHYEEKIKVLDNQIKKDLADKETLENLMQRHEEEAHEKGKILSEQKAMINAMDSKIRSLEQRIVELSEANKLAANSSLFTQRNMKAQEEMISELRQQKFYLETQAGKLEAQNRKLEEQLEKISHQDHSDKNRLLELETRLREVSLEHEEQKLELKRQLTELQLSLQERESQLTALQAARAALESQLRQAKTELEETTAEAEEEIQALTAHRDEIQRKFDALRNSCTVITDLEEQLNQLTEDNAELNNQNFYLSKQLDEASGANDEIVQLRSEVDHLRREITEREMQLTSQKQTMEALKTTCTMLEEQVMDLEALNDELLEKERQWEAWRSVLGDEKSQFECRVRELQRMLDTEKQSRARADQRITESRQVVELAVKEHKAEILALQQALKEQKLKAESLSDKLNDLEKKHAMLEMNARSLQQKLETERELKQRLLEEQAKLQQQMDLQKNHIFRLTQGLQEALDRADLLKTERSDLEYQLENIQVLYSHEKVKMEGTISQQTKLIDFLQAKMDQPAKKKKGLFSRRKEDPALPTQVPLQYNELKVALEKEKARCAELEEALQKTRIELRSAREEAAHRKAADHPHPSTPATARQQIAMSAIVRSPEHQPSALSLLAPPSSRRKESSTPEEFSRRLKERMHHNIPHRFNVGLNMRATKCAVCLDTVHFGRQASKCLECQVMCHPKCSTCLPATCGLPAEYATHFTEAFCRDKMNSPGLQSKEPSSSLHLEGWMKVPRNNKRGQQGWDRKYIVLEGSKVLIYDNEAREAGQRPVEEFELCLPDGDVSIHGAVGASELANTAKADVPYVLKMESHPHTTCWPGRTLYLLAPSFPDKQRWVTALESVVAGGRVSREKAEADAGRDCTSYEPLPAWAQKLLGNSLLKLEGDDRLDMNCTLPFSDQVVLVGTEEGLYALNVLKNSLTHVPGIGAVFQIYIIKDLEKLLMIAGEERALCLVDVKKVKQSLAQSHLPAQPDISPNIFEAVKGCHLFAAGKIESGLCICAAMPSKVVILRYNENLSKYCIRKEIETSEPCSCIHFTNYSILIGTNKFYEIDMKQYTLEEFLDKNDHSLAPAVFASSSNSFPVSIMQVNGAGQREEFLLCFHEFGVFVDSYGRRSRTDDLKWSRLPLAFAYREPYLFVTHFNSLEVIEIQARSSLGTPARAYLEIPNPRYLGPAISSGAIYLASSYQDKLRVICCKGNLVKETGTDHHRGPSTSRSPNKRGPPTYNEHITKRVASSPAPPEGPSHPREPSTPHRYREGRTELRRDKSPGRPLEREKSPGRMLSTRRERSPGRLFEDSSRGRLPVGAVRTPLSQVNKVWDQSSV

Target ORF information:

RefSeq Version	XM_024977740.1
Organism	Bos taurus(cattle)
Definition	Bos taurus citron rho-interacting serine/threonine kinase (CIT), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024977740.1

ORF Insert Sequence:

ATGAACTCAA ATAAGATGGT AAATGCCAAA CTCCCGGTTG GTACTCCAGA TTACATGGCC 
CCTGAAGTGT TGACTGTGAT GAATGGGGAT GGAAAAGGTG CCTATAGCCT AGACTGTGAT 
TGGTGGTCAG TGGGAGTTAT TGCTTATGAG ATGGTTTATG GAAGATGCCC ATTCACTGAA 
GGAACCTCAG CCAAAACCTT CAATAACATC ATGAATTTCC AGCGGTTCTT GAAGTTTCCA 
GATGACCCCA AAGTTAGCAG TGAATTGCTT GATCTAATCC AGAGTTTGTT GTGTGGCCAG 
AAAGAGAGAC TGAAGTTTGA GGGCCTTTGC TGTCATCCTT TCTTCTCCAA AATTGACTGG 
ATTAACATTC GTAACTCTCC TCCCCCCTTC GTTCCCACCC TCAAGTCTGA TGATGACACC 
TCCAATTTTG ATGAACCAGA GAAGAATTCG TGGGTTTCAT CCTCTCCGTG CCAGCTGAAC 
CCCTCAGGTT TCTCGGGCGA AGAACTGCCG TTTGTGGGGT TTTCGTATAG CAAGGCACTG 
GGAATTCTTG GTAGATCTGA GCCTGTTGTA TCAAGTCTGG ACTCCCCTGC CAAGACTAGC 
TCCATGGAAA AGAAACTCCT TATCAAAAGC AAAGAGCTGC AGGACTCCCA GGACAAGTGT 
CACAAGATGG AGCAGGAAAT GACCCGGTTA CATCGGAGAG TGTCAGAGGT GGAGGCTGTG 
CTTAGTCAGA AGGAGGTGGA GCTGAAGGCC TCTGAGACTC AGAGATCCCT CCTGGAGCAG 
GACCTTGCTA CCTACATCAC AGAATGCAGT AGCTTAAAGC GAAGTTTGGA GCAAGCACGG 
ATGGAGGTGT CCCAAGAGGA CGACAAAGCA CTGCAGCTCC TCCATGATAT CAGAGAGCAG 
AGCCGGAAGC TCCAAGAAAT CAAAGAGCAG GAGTACCAGG CTCAAGTGGA AGAAATGAGG 
TTGATGATGA ATCAGTTGGA AGAGGACCTG GTTTCGGCGA GAAGGCGGAG TGACCTCTAT 
GAATCGGAGC TCAGAGAGTC TCGGCTGGCT GCTGAAGAGT TTAAGCGGAA AGCAACAGAA 
TGTCAGCATA AACTGATGAA GGCTAAAGAT CAAGGGAAGC CTGAAGTGGG AGAATATGCC 
AAACTGGAGA AGATCAATGC TGAGCAGCAG CTCAAAATTC AGGAGCTCCA AGAGAAGCTA 
GAAAAGGCTG TGAAAGCTAG CACAGAGGCC ACCGAGCTGC TGCAGAACAT CCGTCAGGCG 
AAGGAGCGGG CCGAGCGTGA GCTGGAGAAG CTGCACAACC GTGAGGACTC CTCCGAGGGC 
ATCAAGAAGA AGCTGGTGGA GGCCGAGGAA CGCCGCCACT CTCTGGAGAA CAAGGTAAAG 
AGGCTAGAGA CCATGGAGCG TAGAGAAAAC AGACTGAAGG ATGACATCCA GACAAAGTCC 
CAACAGATCC AGCAGATGGC TGATAAAATT CTGGAGCTGG AGGAGAAGCA CAGAGAAGCC 
CAGGTCTCAG CGCAGCATCT GGAGGTGCAC CTGAAACAGA AAGAGCAGCA CTATGAAGAA 
AAAATTAAAG TGTTGGACAA TCAGATAAAG AAAGACCTAG CAGACAAGGA GACACTGGAG 
AACCTGATGC AAAGACACGA AGAGGAGGCC CATGAGAAAG GCAAAATTCT CAGCGAGCAG 
AAGGCGATGA TCAATGCTAT GGATTCCAAG ATCAGATCCC TGGAACAAAG GATAGTGGAA 
TTGTCGGAAG CCAATAAACT TGCGGCCAAC AGCAGTCTCT TTACCCAGAG GAACATGAAG 
GCCCAGGAAG AGATGATTTC AGAACTCAGG CAACAGAAGT TTTACCTGGA GACGCAAGCT 
GGGAAATTGG AGGCCCAGAA CCGGAAGCTG GAGGAGCAGC TGGAAAAAAT AAGCCACCAA 
GACCACAGTG ACAAGAACCG TCTGCTGGAG CTGGAGACCA GGCTAAGGGA GGTCAGTCTA 
GAACATGAAG AGCAGAAACT GGAGCTCAAA CGCCAGCTCA CAGAGCTGCA GCTCTCCCTG 
CAGGAGCGTG AATCCCAGCT GACAGCCCTG CAGGCAGCAC GGGCGGCCCT GGAGAGCCAG 
CTCCGCCAGG CCAAGACGGA GCTGGAAGAG ACGACAGCAG AAGCAGAAGA AGAGATCCAG 
GCGCTTACGG CACATAGAGA TGAAATCCAG CGCAAATTCG ATGCCCTTCG TAACAGCTGT 
ACTGTGATCA CGGACTTGGA GGAGCAGCTA AACCAGCTGA CGGAGGACAA TGCCGAGCTC 
AACAACCAAA ACTTCTACTT GTCTAAACAA CTCGATGAGG CCTCTGGTGC CAACGACGAG 
ATTGTGCAGC TGCGAAGTGA AGTGGACCAT CTCCGCCGCG AGATCACTGA GAGGGAGATG 
CAGCTCACTA GCCAGAAGCA AACGATGGAG GCGCTGAAGA CCACGTGCAC AATGCTGGAG 
GAGCAGGTCA TGGACCTGGA GGCCCTGAAC GACGAGCTGC TGGAGAAGGA GCGGCAGTGG 
GAGGCCTGGA GGAGTGTCCT TGGTGACGAG AAGTCCCAGT TTGAGTGTCG GGTTCGAGAG 
TTGCAGAGGA TGCTGGACAC CGAAAAGCAG AGCAGGGCGA GGGCCGACCA GAGGATCACT 
GAGTCGCGCC AGGTGGTCGA GCTGGCAGTA AAGGAGCACA AGGCCGAGAT CCTGGCCTTG 
CAGCAGGCTC TCAAGGAGCA GAAGCTGAAG GCCGAGAGCC TCTCCGACAA GCTCAATGAT 
CTGGAGAAGA AGCACGCCAT GCTTGAAATG AATGCCCGAA GTTTACAACA GAAGCTGGAG 
ACAGAACGAG AGCTCAAACA AAGGCTTCTG GAAGAGCAAG CCAAGTTACA GCAGCAGATG 
GACCTGCAGA AGAATCATAT TTTCCGTCTG ACTCAAGGGC TGCAAGAAGC TCTGGATCGG 
GCTGATCTGC TGAAGACAGA AAGGAGTGAC CTGGAATATC AGCTGGAAAA CATTCAGGTT 
CTCTATTCTC ATGAGAAGGT GAAGATGGAA GGCACTATTT CCCAGCAAAC CAAACTCATC 
GATTTTCTGC AAGCCAAAAT GGACCAGCCT GCCAAAAAGA AAAAGGGTTT ATTTAGTCGA 
CGGAAAGAGG ACCCTGCTTT GCCCACACAG GTTCCTCTGC AGTACAATGA GCTGAAGGTG 
GCTCTGGAGA AGGAGAAAGC TCGCTGCGCG GAGCTCGAGG AAGCCCTGCA GAAGACCCGC 
ATCGAGCTCC GCTCCGCCCG GGAGGAAGCT GCCCACCGGA AAGCTGCAGA CCACCCACAC 
CCATCTACGC CGGCTACCGC CAGGCAGCAG ATCGCCATGT CCGCCATTGT GCGGTCACCC 
GAGCACCAGC CCAGCGCCCT GAGCCTGCTC GCCCCGCCGT CCAGCCGCAG GAAGGAGTCT 
TCCACTCCAG AGGAATTCAG CCGGCGTCTT AAGGAGCGCA TGCACCACAA CATCCCTCAC 
CGGTTCAACG TGGGACTGAA CATGCGAGCC ACGAAGTGCG CCGTGTGTCT GGATACCGTG 
CACTTCGGGC GCCAGGCATC CAAGTGTCTC GAATGTCAGG TCATGTGCCA CCCCAAGTGC 
TCCACATGCT TGCCAGCCAC CTGTGGCCTG CCAGCCGAAT ACGCCACACA CTTCACCGAG 
GCCTTCTGCC GCGACAAAAT GAACTCCCCG GGTCTGCAGT CCAAGGAGCC CAGCAGCAGC 
CTGCACCTGG AAGGGTGGAT GAAGGTGCCC AGGAATAACA AGCGAGGACA GCAAGGCTGG 
GACAGGAAGT ACATCGTTCT GGAGGGCTCC AAAGTCCTCA TTTATGACAA CGAAGCCAGA 
GAAGCTGGAC AGAGGCCGGT GGAAGAATTT GAGCTGTGCC TTCCCGACGG GGACGTGTCT 
ATTCATGGCG CCGTTGGTGC TTCTGAACTT GCAAACACAG CCAAAGCAGA TGTCCCCTAT 
GTCCTGAAGA TGGAGTCTCA CCCCCATACC ACCTGCTGGC CTGGGAGGAC CCTCTACTTG 
CTGGCTCCCA GCTTTCCCGA CAAGCAGCGC TGGGTCACCG CCTTAGAGTC AGTTGTGGCA 
GGTGGGAGAG TGTCTAGGGA AAAGGCGGAA GCCGACGCTG GCCGTGACTG TACTTCCTAC 
GAGCCTCTGC CCGCGTGGGC TCAGAAATTG CTCGGAAACT CCCTGTTGAA ACTGGAAGGT 
GATGACCGTC TGGACATGAA CTGCACGCTG CCCTTCAGCG ACCAGGTGGT GCTGGTGGGC 
ACCGAGGAAG GGCTGTATGC GCTGAATGTC TTGAAGAACT CCCTCACCCA CGTCCCAGGA 
ATCGGAGCAG TCTTCCAGAT TTACATCATC AAGGACCTGG AGAAGCTCCT CATGATCGCA 
GGAGAGGAGC GGGCCCTGTG TCTCGTGGAT GTGAAGAAGG TGAAGCAGTC CCTCGCGCAG 
TCTCACCTCC CCGCCCAGCC AGACATCTCG CCCAACATCT TTGAAGCGGT CAAGGGCTGC 
CACTTGTTTG CTGCTGGCAA GATTGAGAGC GGGCTCTGCA TCTGTGCAGC CATGCCCAGC 
AAAGTTGTCA TTCTCCGCTA CAATGAAAAC CTCAGCAAGT ACTGCATTCG CAAAGAGATC 
GAGACCTCGG AGCCCTGCAG CTGTATCCAC TTCACCAACT ACAGTATCCT CATCGGAACC 
AATAAATTCT ACGAAATCGA CATGAAGCAG TACACGCTAG AGGAATTCCT GGATAAGAAC 
GACCATTCCT TGGCGCCTGC CGTGTTTGCC TCCTCTTCCA ACAGCTTCCC CGTGTCCATC 
ATGCAGGTGA ACGGCGCGGG GCAGCGGGAG GAGTTCCTGC TCTGTTTCCA CGAATTCGGG 
GTGTTCGTGG ATTCTTATGG AAGACGCAGC CGCACAGATG ATCTCAAGTG GAGTCGCTTA 
CCTCTGGCCT TTGCCTACAG AGAACCCTAT CTGTTTGTGA CTCACTTCAA CTCACTCGAA 
GTAATTGAGA TCCAGGCCCG CTCCTCTCTG GGGACCCCCG CCCGAGCATA TTTGGAAATC 
CCCAACCCAC GCTACCTGGG CCCTGCAATC TCCTCAGGAG CCATCTACCT GGCGTCCTCA 
TATCAGGATA AGTTAAGGGT CATCTGCTGC AAAGGAAACC TGGTGAAGGA GACCGGCACC 
GACCACCACC GTGGCCCATC CACCTCCCGC AGCCCCAACA AGCGAGGCCC GCCGACGTAC 
AACGAGCACA TCACCAAGCG CGTGGCCTCA AGCCCGGCGC CTCCCGAAGG CCCCAGCCAC 
CCTCGAGAGC CAAGCACACC CCACCGCTAC CGCGAGGGGC GGACCGAGCT GCGCAGGGAC 
AAGTCTCCGG GCCGCCCCCT GGAGCGCGAG AAGTCCCCCG GCCGGATGCT GAGCACGCGG 
AGGGAGCGCT CCCCCGGGCG GCTGTTTGAG GACAGCAGCA GGGGCCGGCT GCCCGTGGGG 
GCCGTGAGGA CCCCGCTGTC CCAGGTCAAC AAGGTCTGGG ACCAGTCTTC AGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CIT ( XM_024977741.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024833509.1 Bos taurus citron rho-interacting serine/threonine kinase (CIT), transcript variant X9, mRNA.

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CloneID	OBa224629
Clone ID Related Accession (Same CDS sequence)	XM_024977741.1
Accession Version	XM_024977741.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5145bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	citron Rho-interacting kinase isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGAAGT TCAAGTATGG AGCACGGAAT CTACTGGACG CTGGTACTGT TGAATCCATT 
GCCAGCCGGG CCTCCAGGCT GAATCTTTTC TTCCAGGGGA AACCACCCTC CATGACTCAA 
CAGCAGATGT CTCTTCTTTC CCGAGAAGCG ATATTAGATG CCCTCTTTGT TCTCTTTAAA 
GAATGCAGTC AGCCAGCTCT GATGAAGATT AAGCACGTGA GCAACTTTGT CCAAAAGTAT 
TCCGATATCA TAGCTGAGTT GCAAGAGCTC CAGCCTTCAG TGAAGGACTT TGAAGTGAAA 
AGTGTCGTAG GCTGCGGTCA CTTTGCTGAA GTACAGGTGG TCAGAGAGAA AGCGACTGGG 
GATGTCTATG CCATGAAAGT CATGAAGAAG AAGGCCTTGT TGGCCCAGGA GCAGGTCTCA 
TATTTTGAGG AAGAACAGAA CATATTATCT CAGAGTACAA GTCCTTGGAT CCCCCAGTTG 
CAGTATGCCT TTCAGGACAA AAATAACCTT TATCTGGTCA TGGAATATCA GCCTGGAGGG 
GATTTGCTGT CACTTTTGAA TAGATATGAG GACCAATTAG ATGAAAATAT GATTCAGTTT 
TACCTAGCTG AACTGATTTT GGCTGTCCAC AGCGTTCATC AGATGGGCTA TGTACATCGA 
GACATCAAGC CCGAGAACAT CCTCATTGAC CGAACGGGAC ACATCAAGCT GGTGGATTTT 
GGATCAGCTG CTAAAATGAA CTCAAATAAG ATGGTAAATG CCAAACTCCC GGTTGGTACT 
CCAGATTACA TGGCCCCTGA AGTGTTGACT GTGATGAATG GGGATGGAAA AGGTGCCTAT 
AGCCTAGACT GTGATTGGTG GTCAGTGGGA GTTATTGCTT ATGAGATGGT TTATGGAAGA 
TGCCCATTCA CTGAAGGAAC CTCAGCCAAA ACCTTCAATA ACATCATGAA TTTCCAGCGG 
TTCTTGAAGT TTCCAGATGA CCCCAAAGTT AGCAGTGAAT TGCTTGATCT AATCCAGAGT 
TTGTTGTGTG GCCAGAAAGA GAGACTGAAG TTTGAGGGCC TTTGCTGTCA TCCTTTCTTC 
TCCAAAATTG ACTGGATTAA CATTCGTAAC TCTCCTCCCC CCTTCGTTCC CACCCTCAAG 
TCTGATGATG ACACCTCCAA TTTTGATGAA CCAGAGAAGA ATTCGTGGGT TTCATCCTCT 
CCGTGCCAGC TGAACCCCTC AGGTTTCTCG GGCGAAGAAC TGCCGTTTGT GGGGTTTTCG 
TATAGCAAGG CACTGGGAAT TCTTGGTAGA TCTGAGCCTG TTGTATCAAG TCTGGACTCC 
CCTGCCAAGA CTAGCTCCAT GGAAAAGAAA CTCCTTATCA AAAGCAAAGA GCTGCAGGAC 
TCCCAGGACA AGTGTCACAA GATGGAGCAG GAAATGACCC GGTTACATCG GAGAGTGTCA 
GAGGTGGAGG CTGTGCTTAG TCAGAAGGAG GTGGAGCTGA AGGCCTCTGA GACTCAGAGA 
TCCCTCCTGG AGCAGGACCT TGCTACCTAC ATCACAGAAT GCAGTAGCTT AAAGCGAAGT 
TTGGAGCAAG CACGGATGGA GGTGTCCCAA GAGGACGACA AAGCACTGCA GCTCCTCCAT 
GATATCAGAG AGCAGAGCCG GAAGCTCCAA GAAATCAAAG AGCAGGAGTA CCAGGCTCAA 
GTGGAAGAAA TGAGGTTGAT GATGAATCAG TTGGAAGAGG ACCTGGTTTC GGCGAGAAGG 
CGGAGTGACC TCTATGAATC GGAGCTCAGA GAGTCTCGGC TGGCTGCTGA AGAGTTTAAG 
CGGAAAGCAA CAGAATGTCA GCATAAACTG ATGAAGGCTA AAGATCAAGG GAAGCCTGAA 
GTGGGAGAAT ATGCCAAACT GGAGAAGATC AATGCTGAGC AGCAGCTCAA AATTCAGGAG 
CTCCAAGAGA AGCTAGAAAA GGCTGTGAAA GCTAGCACAG AGGCCACCGA GCTGCTGCAG 
AACATCCGTC AGGCGAAGGA GCGGGCCGAG CGTGAGCTGG AGAAGCTGCA CAACCGTGAG 
GACTCCTCCG AGGGCATCAA GAAGAAGCTG GTGGAGGCCG AGGAACGCCG CCACTCTCTG 
GAGAACAAGG TAAAGAGGCT AGAGACCATG GAGCGTAGAG AAAACAGACT GAAGGATGAC 
ATCCAGACAA AGTCCCAACA GATCCAGCAG ATGGCTGATA AAATTCTGGA GCTGGAGGAG 
AAGCACAGAG AAGCCCAGGT CTCAGCGCAG CATCTGGAGG TGCACCTGAA ACAGAAAGAG 
CAGCACTATG AAGAAAAAAT TAAAGTGTTG GACAATCAGA TAAAGAAAGA CCTAGCAGAC 
AAGGAGACAC TGGAGAACCT GATGCAAAGA CACGAAGAGG AGGCCCATGA GAAAGGCAAA 
ATTCTCAGCG AGCAGAAGGC GATGATCAAT GCTATGGATT CCAAGATCAG ATCCCTGGAA 
CAAAGGATAG TGGAATTGTC GGAAGCCAAT AAACTTGCGG CCAACAGCAG TCTCTTTACC 
CAGAGGAACA TGAAGGCCCA GGAAGAGATG ATTTCAGAAC TCAGGCAACA GAAGTTTTAC 
CTGGAGACGC AAGCTGGGAA ATTGGAGGCC CAGAACCGGA AGCTGGAGGA GCAGCTGGAA 
AAAATAAGCC ACCAAGACCA CAGTGACAAG AACCGTCTGC TGGAGCTGGA GACCAGGCTA 
AGGGAGGTCA GTCTAGAACA TGAAGAGCAG AAACTGGAGC TCAAACGCCA GCTCACAGAG 
CTGCAGCTCT CCCTGCAGGA GCGTGAATCC CAGCTGACAG CCCTGCAGGC AGCACGGGCG 
GCCCTGGAGA GCCAGCTCCG CCAGGCCAAG ACGGAGCTGG AAGAGACGAC AGCAGAAGCA 
GAAGAAGAGA TCCAGGCGCT TACGGCACAT AGAGATGAAA TCCAGCGCAA ATTCGATGCC 
CTTCGTAACA GCTGTACTGT GATCACGGAC TTGGAGGAGC AGCTAAACCA GCTGACGGAG 
GACAATGCCG AGCTCAACAA CCAAAACTTC TACTTGTCTA AACAACTCGA TGAGGCCTCT 
GGTGCCAACG ACGAGATTGT GCAGCTGCGA AGTGAAGTGG ACCATCTCCG CCGCGAGATC 
ACTGAGAGGG AGATGCAGCT CACTAGCCAG AAGCAAACGA TGGAGGCGCT GAAGACCACG 
TGCACAATGC TGGAGGAGCA GGTCATGGAC CTGGAGGCCC TGAACGACGA GCTGCTGGAG 
AAGGAGCGGC AGTGGGAGGC CTGGAGGAGT GTCCTTGGTG ACGAGAAGTC CCAGTTTGAG 
TGTCGGGTTC GAGAGTTGCA GAGGATGCTG GACACCGAAA AGCAGAGCAG GGCGAGGGCC 
GACCAGAGGA TCACTGAGTC GCGCCAGGTG GTCGAGCTGG CAGTAAAGGA GCACAAGGCC 
GAGATCCTGG CCTTGCAGCA GGCTCTCAAG GAGCAGAAGC TGAAGGCCGA GAGCCTCTCC 
GACAAGCTCA ATGATCTGGA GAAGAAGCAC GCCATGCTTG AAATGAATGC CCGAAGTTTA 
CAACAGAAGC TGGAGACAGA ACGAGAGCTC AAACAAAGGC TTCTGGAAGA GCAAGCCAAG 
TTACAGCAGC AGATGGACCT GCAGAAGAAT CATATTTTCC GTCTGACTCA AGGGCTGCAA 
GAAGCTCTGG ATCGGGCTGA TCTGCTGAAG ACAGAAAGGA GTGACCTGGA ATATCAGCTG 
GAAAACATTC AGGTTCTCTA TTCTCATGAG AAGGTGAAGA TGGAAGGCAC TATTTCCCAG 
CAAACCAAAC TCATCGATTT TCTGCAAGCC AAAATGGACC AGCCTGCCAA AAAGAAAAAG 
GGTTTATTTA GTCGACGGAA AGAGGACCCT GCTTTGCCCA CACAGGTTCC TCTGCAGTAC 
AATGAGCTGA AGGTGGCTCT GGAGAAGGAG AAAGCTCGCT GCGCGGAGCT CGAGGAAGCC 
CTGCAGAAGA CCCGCATCGA GCTCCGCTCC GCCCGGGAGG AAGCTGCCCA CCGGAAAGCT 
GCAGACCACC CACACCCATC TACGCCGGCT ACCGCCAGGC AGCAGATCGC CATGTCCGCC 
ATTGTGCGGT CACCCGAGCA CCAGCCCAGC GCCCTGAGCC TGCTCGCCCC GCCGTCCAGC 
CGCAGGAAGG AGTCTTCCAC TCCAGAGGAA TTCAGCCGGC GTCTTAAGGA GCGCATGCAC 
CACAACATCC CTCACCGGTT CAACGTGGGA CTGAACATGC GAGCCACGAA GTGCGCCGTG 
TGTCTGGATA CCGTGCACTT CGGGCGCCAG GCATCCAAGT GTCTCGAATG TCAGGTCATG 
TGCCACCCCA AGTGCTCCAC ATGCTTGCCA GCCACCTGTG GCCTGCCAGC CGAATACGCC 
ACACACTTCA CCGAGGCCTT CTGCCGCGAC AAAATGAACT CCCCGGGTCT GCAGTCCAAG 
GAGCCCAGCA GCAGCCTGCA CCTGGAAGGG TGGATGAAGG TGCCCAGGAA TAACAAGCGA 
GGACAGCAAG GCTGGGACAG GAAGTACATC GTTCTGGAGG GCTCCAAAGT CCTCATTTAT 
GACAACGAAG CCAGAGAAGC TGGACAGAGG CCGGTGGAAG AATTTGAGCT GTGCCTTCCC 
GACGGGGACG TGTCTATTCA TGGCGCCGTT GGTGCTTCTG AACTTGCAAA CACAGCCAAA 
GCAGATGTCC CCTATGTCCT GAAGATGGAG TCTCACCCCC ATACCACCTG CTGGCCTGGG 
AGGACCCTCT ACTTGCTGGC TCCCAGCTTT CCCGACAAGC AGCGCTGGGT CACCGCCTTA 
GAGTCAGTTG TGGCAGGTGG GAGAGTGTCT AGGGAAAAGG CGGAAGCCGA CGCTGGCCGT 
GACTGTACTT CCTACGAGCC TCTGCCCGCG TGGGCTCAGA AATTGCTCGG AAACTCCCTG 
TTGAAACTGG AAGGTGATGA CCGTCTGGAC ATGAACTGCA CGCTGCCCTT CAGCGACCAG 
GTGGTGCTGG TGGGCACCGA GGAAGGGCTG TATGCGCTGA ATGTCTTGAA GAACTCCCTC 
ACCCACGTCC CAGGAATCGG AGCAGTCTTC CAGATTTACA TCATCAAGGA CCTGGAGAAG 
CTCCTCATGA TCGCAGATCT AACACTTGGA CCTAAACTGT GTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024833509.1
CDS	113..5257
Translation	MLKFKYGARNLLDAGTVESIASRASRLNLFFQGKPPSMTQQQMSLLSREAILDALFVLFKECSQPALMKIKHVSNFVQKYSDIIAELQELQPSVKDFEVKSVVGCGHFAEVQVVREKATGDVYAMKVMKKKALLAQEQVSYFEEEQNILSQSTSPWIPQLQYAFQDKNNLYLVMEYQPGGDLLSLLNRYEDQLDENMIQFYLAELILAVHSVHQMGYVHRDIKPENILIDRTGHIKLVDFGSAAKMNSNKMVNAKLPVGTPDYMAPEVLTVMNGDGKGAYSLDCDWWSVGVIAYEMVYGRCPFTEGTSAKTFNNIMNFQRFLKFPDDPKVSSELLDLIQSLLCGQKERLKFEGLCCHPFFSKIDWINIRNSPPPFVPTLKSDDDTSNFDEPEKNSWVSSSPCQLNPSGFSGEELPFVGFSYSKALGILGRSEPVVSSLDSPAKTSSMEKKLLIKSKELQDSQDKCHKMEQEMTRLHRRVSEVEAVLSQKEVELKASETQRSLLEQDLATYITECSSLKRSLEQARMEVSQEDDKALQLLHDIREQSRKLQEIKEQEYQAQVEEMRLMMNQLEEDLVSARRRSDLYESELRESRLAAEEFKRKATECQHKLMKAKDQGKPEVGEYAKLEKINAEQQLKIQELQEKLEKAVKASTEATELLQNIRQAKERAERELEKLHNREDSSEGIKKKLVEAEERRHSLENKVKRLETMERRENRLKDDIQTKSQQIQQMADKILELEEKHREAQVSAQHLEVHLKQKEQHYEEKIKVLDNQIKKDLADKETLENLMQRHEEEAHEKGKILSEQKAMINAMDSKIRSLEQRIVELSEANKLAANSSLFTQRNMKAQEEMISELRQQKFYLETQAGKLEAQNRKLEEQLEKISHQDHSDKNRLLELETRLREVSLEHEEQKLELKRQLTELQLSLQERESQLTALQAARAALESQLRQAKTELEETTAEAEEEIQALTAHRDEIQRKFDALRNSCTVITDLEEQLNQLTEDNAELNNQNFYLSKQLDEASGANDEIVQLRSEVDHLRREITEREMQLTSQKQTMEALKTTCTMLEEQVMDLEALNDELLEKERQWEAWRSVLGDEKSQFECRVRELQRMLDTEKQSRARADQRITESRQVVELAVKEHKAEILALQQALKEQKLKAESLSDKLNDLEKKHAMLEMNARSLQQKLETERELKQRLLEEQAKLQQQMDLQKNHIFRLTQGLQEALDRADLLKTERSDLEYQLENIQVLYSHEKVKMEGTISQQTKLIDFLQAKMDQPAKKKKGLFSRRKEDPALPTQVPLQYNELKVALEKEKARCAELEEALQKTRIELRSAREEAAHRKAADHPHPSTPATARQQIAMSAIVRSPEHQPSALSLLAPPSSRRKESSTPEEFSRRLKERMHHNIPHRFNVGLNMRATKCAVCLDTVHFGRQASKCLECQVMCHPKCSTCLPATCGLPAEYATHFTEAFCRDKMNSPGLQSKEPSSSLHLEGWMKVPRNNKRGQQGWDRKYIVLEGSKVLIYDNEAREAGQRPVEEFELCLPDGDVSIHGAVGASELANTAKADVPYVLKMESHPHTTCWPGRTLYLLAPSFPDKQRWVTALESVVAGGRVSREKAEADAGRDCTSYEPLPAWAQKLLGNSLLKLEGDDRLDMNCTLPFSDQVVLVGTEEGLYALNVLKNSLTHVPGIGAVFQIYIIKDLEKLLMIADLTLGPKLC

Target ORF information:

RefSeq Version	XM_024977741.1
Organism	Bos taurus(cattle)
Definition	Bos taurus citron rho-interacting serine/threonine kinase (CIT), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024977741.1

ORF Insert Sequence:

ATGTTGAAGT TCAAGTATGG AGCACGGAAT CTACTGGACG CTGGTACTGT TGAATCCATT 
GCCAGCCGGG CCTCCAGGCT GAATCTTTTC TTCCAGGGGA AACCACCCTC CATGACTCAA 
CAGCAGATGT CTCTTCTTTC CCGAGAAGCG ATATTAGATG CCCTCTTTGT TCTCTTTAAA 
GAATGCAGTC AGCCAGCTCT GATGAAGATT AAGCACGTGA GCAACTTTGT CCAAAAGTAT 
TCCGATATCA TAGCTGAGTT GCAAGAGCTC CAGCCTTCAG TGAAGGACTT TGAAGTGAAA 
AGTGTCGTAG GCTGCGGTCA CTTTGCTGAA GTACAGGTGG TCAGAGAGAA AGCGACTGGG 
GATGTCTATG CCATGAAAGT CATGAAGAAG AAGGCCTTGT TGGCCCAGGA GCAGGTCTCA 
TATTTTGAGG AAGAACAGAA CATATTATCT CAGAGTACAA GTCCTTGGAT CCCCCAGTTG 
CAGTATGCCT TTCAGGACAA AAATAACCTT TATCTGGTCA TGGAATATCA GCCTGGAGGG 
GATTTGCTGT CACTTTTGAA TAGATATGAG GACCAATTAG ATGAAAATAT GATTCAGTTT 
TACCTAGCTG AACTGATTTT GGCTGTCCAC AGCGTTCATC AGATGGGCTA TGTACATCGA 
GACATCAAGC CCGAGAACAT CCTCATTGAC CGAACGGGAC ACATCAAGCT GGTGGATTTT 
GGATCAGCTG CTAAAATGAA CTCAAATAAG ATGGTAAATG CCAAACTCCC GGTTGGTACT 
CCAGATTACA TGGCCCCTGA AGTGTTGACT GTGATGAATG GGGATGGAAA AGGTGCCTAT 
AGCCTAGACT GTGATTGGTG GTCAGTGGGA GTTATTGCTT ATGAGATGGT TTATGGAAGA 
TGCCCATTCA CTGAAGGAAC CTCAGCCAAA ACCTTCAATA ACATCATGAA TTTCCAGCGG 
TTCTTGAAGT TTCCAGATGA CCCCAAAGTT AGCAGTGAAT TGCTTGATCT AATCCAGAGT 
TTGTTGTGTG GCCAGAAAGA GAGACTGAAG TTTGAGGGCC TTTGCTGTCA TCCTTTCTTC 
TCCAAAATTG ACTGGATTAA CATTCGTAAC TCTCCTCCCC CCTTCGTTCC CACCCTCAAG 
TCTGATGATG ACACCTCCAA TTTTGATGAA CCAGAGAAGA ATTCGTGGGT TTCATCCTCT 
CCGTGCCAGC TGAACCCCTC AGGTTTCTCG GGCGAAGAAC TGCCGTTTGT GGGGTTTTCG 
TATAGCAAGG CACTGGGAAT TCTTGGTAGA TCTGAGCCTG TTGTATCAAG TCTGGACTCC 
CCTGCCAAGA CTAGCTCCAT GGAAAAGAAA CTCCTTATCA AAAGCAAAGA GCTGCAGGAC 
TCCCAGGACA AGTGTCACAA GATGGAGCAG GAAATGACCC GGTTACATCG GAGAGTGTCA 
GAGGTGGAGG CTGTGCTTAG TCAGAAGGAG GTGGAGCTGA AGGCCTCTGA GACTCAGAGA 
TCCCTCCTGG AGCAGGACCT TGCTACCTAC ATCACAGAAT GCAGTAGCTT AAAGCGAAGT 
TTGGAGCAAG CACGGATGGA GGTGTCCCAA GAGGACGACA AAGCACTGCA GCTCCTCCAT 
GATATCAGAG AGCAGAGCCG GAAGCTCCAA GAAATCAAAG AGCAGGAGTA CCAGGCTCAA 
GTGGAAGAAA TGAGGTTGAT GATGAATCAG TTGGAAGAGG ACCTGGTTTC GGCGAGAAGG 
CGGAGTGACC TCTATGAATC GGAGCTCAGA GAGTCTCGGC TGGCTGCTGA AGAGTTTAAG 
CGGAAAGCAA CAGAATGTCA GCATAAACTG ATGAAGGCTA AAGATCAAGG GAAGCCTGAA 
GTGGGAGAAT ATGCCAAACT GGAGAAGATC AATGCTGAGC AGCAGCTCAA AATTCAGGAG 
CTCCAAGAGA AGCTAGAAAA GGCTGTGAAA GCTAGCACAG AGGCCACCGA GCTGCTGCAG 
AACATCCGTC AGGCGAAGGA GCGGGCCGAG CGTGAGCTGG AGAAGCTGCA CAACCGTGAG 
GACTCCTCCG AGGGCATCAA GAAGAAGCTG GTGGAGGCCG AGGAACGCCG CCACTCTCTG 
GAGAACAAGG TAAAGAGGCT AGAGACCATG GAGCGTAGAG AAAACAGACT GAAGGATGAC 
ATCCAGACAA AGTCCCAACA GATCCAGCAG ATGGCTGATA AAATTCTGGA GCTGGAGGAG 
AAGCACAGAG AAGCCCAGGT CTCAGCGCAG CATCTGGAGG TGCACCTGAA ACAGAAAGAG 
CAGCACTATG AAGAAAAAAT TAAAGTGTTG GACAATCAGA TAAAGAAAGA CCTAGCAGAC 
AAGGAGACAC TGGAGAACCT GATGCAAAGA CACGAAGAGG AGGCCCATGA GAAAGGCAAA 
ATTCTCAGCG AGCAGAAGGC GATGATCAAT GCTATGGATT CCAAGATCAG ATCCCTGGAA 
CAAAGGATAG TGGAATTGTC GGAAGCCAAT AAACTTGCGG CCAACAGCAG TCTCTTTACC 
CAGAGGAACA TGAAGGCCCA GGAAGAGATG ATTTCAGAAC TCAGGCAACA GAAGTTTTAC 
CTGGAGACGC AAGCTGGGAA ATTGGAGGCC CAGAACCGGA AGCTGGAGGA GCAGCTGGAA 
AAAATAAGCC ACCAAGACCA CAGTGACAAG AACCGTCTGC TGGAGCTGGA GACCAGGCTA 
AGGGAGGTCA GTCTAGAACA TGAAGAGCAG AAACTGGAGC TCAAACGCCA GCTCACAGAG 
CTGCAGCTCT CCCTGCAGGA GCGTGAATCC CAGCTGACAG CCCTGCAGGC AGCACGGGCG 
GCCCTGGAGA GCCAGCTCCG CCAGGCCAAG ACGGAGCTGG AAGAGACGAC AGCAGAAGCA 
GAAGAAGAGA TCCAGGCGCT TACGGCACAT AGAGATGAAA TCCAGCGCAA ATTCGATGCC 
CTTCGTAACA GCTGTACTGT GATCACGGAC TTGGAGGAGC AGCTAAACCA GCTGACGGAG 
GACAATGCCG AGCTCAACAA CCAAAACTTC TACTTGTCTA AACAACTCGA TGAGGCCTCT 
GGTGCCAACG ACGAGATTGT GCAGCTGCGA AGTGAAGTGG ACCATCTCCG CCGCGAGATC 
ACTGAGAGGG AGATGCAGCT CACTAGCCAG AAGCAAACGA TGGAGGCGCT GAAGACCACG 
TGCACAATGC TGGAGGAGCA GGTCATGGAC CTGGAGGCCC TGAACGACGA GCTGCTGGAG 
AAGGAGCGGC AGTGGGAGGC CTGGAGGAGT GTCCTTGGTG ACGAGAAGTC CCAGTTTGAG 
TGTCGGGTTC GAGAGTTGCA GAGGATGCTG GACACCGAAA AGCAGAGCAG GGCGAGGGCC 
GACCAGAGGA TCACTGAGTC GCGCCAGGTG GTCGAGCTGG CAGTAAAGGA GCACAAGGCC 
GAGATCCTGG CCTTGCAGCA GGCTCTCAAG GAGCAGAAGC TGAAGGCCGA GAGCCTCTCC 
GACAAGCTCA ATGATCTGGA GAAGAAGCAC GCCATGCTTG AAATGAATGC CCGAAGTTTA 
CAACAGAAGC TGGAGACAGA ACGAGAGCTC AAACAAAGGC TTCTGGAAGA GCAAGCCAAG 
TTACAGCAGC AGATGGACCT GCAGAAGAAT CATATTTTCC GTCTGACTCA AGGGCTGCAA 
GAAGCTCTGG ATCGGGCTGA TCTGCTGAAG ACAGAAAGGA GTGACCTGGA ATATCAGCTG 
GAAAACATTC AGGTTCTCTA TTCTCATGAG AAGGTGAAGA TGGAAGGCAC TATTTCCCAG 
CAAACCAAAC TCATCGATTT TCTGCAAGCC AAAATGGACC AGCCTGCCAA AAAGAAAAAG 
GGTTTATTTA GTCGACGGAA AGAGGACCCT GCTTTGCCCA CACAGGTTCC TCTGCAGTAC 
AATGAGCTGA AGGTGGCTCT GGAGAAGGAG AAAGCTCGCT GCGCGGAGCT CGAGGAAGCC 
CTGCAGAAGA CCCGCATCGA GCTCCGCTCC GCCCGGGAGG AAGCTGCCCA CCGGAAAGCT 
GCAGACCACC CACACCCATC TACGCCGGCT ACCGCCAGGC AGCAGATCGC CATGTCCGCC 
ATTGTGCGGT CACCCGAGCA CCAGCCCAGC GCCCTGAGCC TGCTCGCCCC GCCGTCCAGC 
CGCAGGAAGG AGTCTTCCAC TCCAGAGGAA TTCAGCCGGC GTCTTAAGGA GCGCATGCAC 
CACAACATCC CTCACCGGTT CAACGTGGGA CTGAACATGC GAGCCACGAA GTGCGCCGTG 
TGTCTGGATA CCGTGCACTT CGGGCGCCAG GCATCCAAGT GTCTCGAATG TCAGGTCATG 
TGCCACCCCA AGTGCTCCAC ATGCTTGCCA GCCACCTGTG GCCTGCCAGC CGAATACGCC 
ACACACTTCA CCGAGGCCTT CTGCCGCGAC AAAATGAACT CCCCGGGTCT GCAGTCCAAG 
GAGCCCAGCA GCAGCCTGCA CCTGGAAGGG TGGATGAAGG TGCCCAGGAA TAACAAGCGA 
GGACAGCAAG GCTGGGACAG GAAGTACATC GTTCTGGAGG GCTCCAAAGT CCTCATTTAT 
GACAACGAAG CCAGAGAAGC TGGACAGAGG CCGGTGGAAG AATTTGAGCT GTGCCTTCCC 
GACGGGGACG TGTCTATTCA TGGCGCCGTT GGTGCTTCTG AACTTGCAAA CACAGCCAAA 
GCAGATGTCC CCTATGTCCT GAAGATGGAG TCTCACCCCC ATACCACCTG CTGGCCTGGG 
AGGACCCTCT ACTTGCTGGC TCCCAGCTTT CCCGACAAGC AGCGCTGGGT CACCGCCTTA 
GAGTCAGTTG TGGCAGGTGG GAGAGTGTCT AGGGAAAAGG CGGAAGCCGA CGCTGGCCGT 
GACTGTACTT CCTACGAGCC TCTGCCCGCG TGGGCTCAGA AATTGCTCGG AAACTCCCTG 
TTGAAACTGG AAGGTGATGA CCGTCTGGAC ATGAACTGCA CGCTGCCCTT CAGCGACCAG 
GTGGTGCTGG TGGGCACCGA GGAAGGGCTG TATGCGCTGA ATGTCTTGAA GAACTCCCTC 
ACCCACGTCC CAGGAATCGG AGCAGTCTTC CAGATTTACA TCATCAAGGA CCTGGAGAAG 
CTCCTCATGA TCGCAGATCT AACACTTGGA CCTAAACTGT GTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CIT ( XM_024977743.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024833511.1 Bos taurus citron rho-interacting serine/threonine kinase (CIT), transcript variant X11, mRNA.

Price & Availability↑

CloneID	OBa224630
Clone ID Related Accession (Same CDS sequence)	XM_024977743.1 , XM_024977742.1
Accession Version	XM_024977743.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4914bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	citron Rho-interacting kinase isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAAGA AACTCCTTAT CAAAAGCAAA GAGCTGCAGG ACTCCCAGGA CAAGTGTCAC 
AAGATGGAGC AGGAAATGAC CCGGTTACAT CGGAGAGTGT CAGAGGTGGA GGCTGTGCTT 
AGTCAGAAGG AGGTGGAGCT GAAGGCCTCT GAGACTCAGA GATCCCTCCT GGAGCAGGAC 
CTTGCTACCT ACATCACAGA ATGCAGTAGC TTAAAGCGAA GTTTGGAGCA AGCACGGATG 
GAGGTGTCCC AAGAGGACGA CAAAGCACTG CAGCTCCTCC ATGATATCAG AGAGCAGAGC 
CGGAAGCTCC AAGAAATCAA AGAGCAGGAG TACCAGGCTC AAGTGGAAGA AATGAGGTTG 
ATGATGAATC AGTTGGAAGA GGACCTGGTT TCGGCGAGAA GGCGGAGTGA CCTCTATGAA 
TCGGAGCTCA GAGAGTCTCG GCTGGCTGCT GAAGAGTTTA AGCGGAAAGC AACAGAATGT 
CAGCATAAAC TGATGAAGGC TAAAGATCAA GGGAAGCCTG AAGTGGGAGA ATATGCCAAA 
CTGGAGAAGA TCAATGCTGA GCAGCAGCTC AAAATTCAGG AGCTCCAAGA GAAGCTAGAA 
AAGGCTGTGA AAGCTAGCAC AGAGGCCACC GAGCTGCTGC AGAACATCCG TCAGGCGAAG 
GAGCGGGCCG AGCGTGAGCT GGAGAAGCTG CACAACCGTG AGGACTCCTC CGAGGGCATC 
AAGAAGAAGC TGGTGGAGGC CGAGGAACGC CGCCACTCTC TGGAGAACAA GGTAAAGAGG 
CTAGAGACCA TGGAGCGTAG AGAAAACAGA CTGAAGGATG ACATCCAGAC AAAGTCCCAA 
CAGATCCAGC AGATGGCTGA TAAAATTCTG GAGCTGGAGG AGAAGCACAG AGAAGCCCAG 
GTCTCAGCGC AGCATCTGGA GGTGCACCTG AAACAGAAAG AGCAGCACTA TGAAGAAAAA 
ATTAAAGTGT TGGACAATCA GATAAAGAAA GACCTAGCAG ACAAGGAGAC ACTGGAGAAC 
CTGATGCAAA GACACGAAGA GGAGGCCCAT GAGAAAGGCA AAATTCTCAG CGAGCAGAAG 
GCGATGATCA ATGCTATGGA TTCCAAGATC AGATCCCTGG AACAAAGGAT AGTGGAATTG 
TCGGAAGCCA ATAAACTTGC GGCCAACAGC AGTCTCTTTA CCCAGAGGAA CATGAAGGCC 
CAGGAAGAGA TGATTTCAGA ACTCAGGCAA CAGAAGTTTT ACCTGGAGAC GCAAGCTGGG 
AAATTGGAGG CCCAGAACCG GAAGCTGGAG GAGCAGCTGG AAAAAATAAG CCACCAAGAC 
CACAGTGACA AGAACCGTCT GCTGGAGCTG GAGACCAGGC TAAGGGAGGT CAGTCTAGAA 
CATGAAGAGC AGAAACTGGA GCTCAAACGC CAGCTCACAG AGCTGCAGCT CTCCCTGCAG 
GAGCGTGAAT CCCAGCTGAC AGCCCTGCAG GCAGCACGGG CGGCCCTGGA GAGCCAGCTC 
CGCCAGGCCA AGACGGAGCT GGAAGAGACG ACAGCAGAAG CAGAAGAAGA GATCCAGGCG 
CTTACGGCAC ATAGAGATGA AATCCAGCGC AAATTCGATG CCCTTCGTAA CAGCTGTACT 
GTGATCACGG ACTTGGAGGA GCAGCTAAAC CAGCTGACGG AGGACAATGC CGAGCTCAAC 
AACCAAAACT TCTACTTGTC TAAACAACTC GATGAGGCCT CTGGTGCCAA CGACGAGATT 
GTGCAGCTGC GAAGTGAAGT GGACCATCTC CGCCGCGAGA TCACTGAGAG GGAGATGCAG 
CTCACTAGCC AGAAGCAAAC GATGGAGGCG CTGAAGACCA CGTGCACAAT GCTGGAGGAG 
CAGGTCATGG ACCTGGAGGC CCTGAACGAC GAGCTGCTGG AGAAGGAGCG GCAGTGGGAG 
GCCTGGAGGA GTGTCCTTGG TGACGAGAAG TCCCAGTTTG AGTGTCGGGT TCGAGAGTTG 
CAGAGGATGC TGGACACCGA AAAGCAGAGC AGGGCGAGGG CCGACCAGAG GATCACTGAG 
TCGCGCCAGG TGGTCGAGCT GGCAGTAAAG GAGCACAAGG CCGAGATCCT GGCCTTGCAG 
CAGGCTCTCA AGGAGCAGAA GCTGAAGGCC GAGAGCCTCT CCGACAAGCT CAATGATCTG 
GAGAAGAAGC ACGCCATGCT TGAAATGAAT GCCCGAAGTT TACAACAGAA GCTGGAGACA 
GAACGAGAGC TCAAACAAAG GCTTCTGGAA GAGCAAGCCA AGTTACAGCA GCAGATGGAC 
CTGCAGAAGA ATCATATTTT CCGTCTGACT CAAGGGCTGC AAGAAGCTCT GGATCGGGCT 
GATCTGCTGA AGACAGAAAG GAGTGACCTG GAATATCAGC TGGAAAACAT TCAGGTTCTC 
TATTCTCATG AGAAGGTGAA GATGGAAGGC ACTATTTCCC AGCAAACCAA ACTCATCGAT 
TTTCTGCAAG CCAAAATGGA CCAGCCTGCC AAAAAGAAAA AGGGTTTATT TAGTCGACGG 
AAAGAGGACC CTGCTTTGCC CACACAGGTT CCTCTGCAGT ACAATGAGCT GAAGGTGGCT 
CTGGAGAAGG AGAAAGCTCG CTGCGCGGAG CTCGAGGAAG CCCTGCAGAA GACCCGCATC 
GAGCTCCGCT CCGCCCGGGA GGAAGCTGCC CACCGGAAAG CTGCAGACCA CCCACACCCA 
TCTACGCCGG CTACCGCCAG GCAGCAGATC GCCATGTCCG CCATTGTGCG GTCACCCGAG 
CACCAGCCCA GCGCCCTGAG CCTGCTCGCC CCGCCGTCCA GCCGCAGGAA GGAGTCTTCC 
ACTCCAGAGG AATTCAGCCG GCGTCTTAAG GAGCGCATGC ACCACAACAT CCCTCACCGG 
TTCAACGTGG GACTGAACAT GCGAGCCACG AAGTGCGCCG TGTGTCTGGA TACCGTGCAC 
TTCGGGCGCC AGGCATCCAA GTGTCTCGAA TGTCAGGTCA TGTGCCACCC CAAGTGCTCC 
ACATGCTTGC CAGCCACCTG TGGCCTGCCA GCCGAATACG CCACACACTT CACCGAGGCC 
TTCTGCCGCG ACAAAATGAA CTCCCCGGGT CTGCAGTCCA AGGAGCCCAG CAGCAGCCTG 
CACCTGGAAG GGTGGATGAA GGTGCCCAGG AATAACAAGC GAGGACAGCA AGGCTGGGAC 
AGGAAGTACA TCGTTCTGGA GGGCTCCAAA GTCCTCATTT ATGACAACGA AGCCAGAGAA 
GCTGGACAGA GGCCGGTGGA AGAATTTGAG CTGTGCCTTC CCGACGGGGA CGTGTCTATT 
CATGGCGCCG TTGGTGCTTC TGAACTTGCA AACACAGCCA AAGCAGATGT CCCCTATGTC 
CTGAAGATGG AGTCTCACCC CCATACCACC TGCTGGCCTG GGAGGACCCT CTACTTGCTG 
GCTCCCAGCT TTCCCGACAA GCAGCGCTGG GTCACCGCCT TAGAGTCAGT TGTGGCAGGT 
GGGAGAGTGT CTAGGGAAAA GGCGGAAGCC GACGCTGGCC GTGACTGTAC TTCCTACGAG 
CCTCTGCCCG CGTGGGCTCA GAAATTGCTC GGAAACTCCC TGTTGAAACT GGAAGGTGAT 
GACCGTCTGG ACATGAACTG CACGCTGCCC TTCAGCGACC AGGTGGTGCT GGTGGGCACC 
GAGGAAGGGC TGTATGCGCT GAATGTCTTG AAGAACTCCC TCACCCACGT CCCAGGAATC 
GGAGCAGTCT TCCAGATTTA CATCATCAAG GACCTGGAGA AGCTCCTCAT GATCGCAGGA 
GAGGAGCGGG CCCTGTGTCT CGTGGATGTG AAGAAGGTGA AGCAGTCCCT CGCGCAGTCT 
CACCTCCCCG CCCAGCCAGA CATCTCGCCC AACATCTTTG AAGCGGTCAA GGGCTGCCAC 
TTGTTTGCTG CTGGCAAGAT TGAGAGCGGG CTCTGCATCT GTGCAGCCAT GCCCAGCAAA 
GTTGTCATTC TCCGCTACAA TGAAAACCTC AGCAAGTACT GCATTCGCAA AGAGATCGAG 
ACCTCGGAGC CCTGCAGCTG TATCCACTTC ACCAACTACA GTATCCTCAT CGGAACCAAT 
AAATTCTACG AAATCGACAT GAAGCAGTAC ACGCTAGAGG AATTCCTGGA TAAGAACGAC 
CATTCCTTGG CGCCTGCCGT GTTTGCCTCC TCTTCCAACA GCTTCCCCGT GTCCATCATG 
CAGGTGAACG GCGCGGGGCA GCGGGAGGAG TTCCTGCTCT GTTTCCACGA ATTCGGGGTG 
TTCGTGGATT CTTATGGAAG ACGCAGCCGC ACAGATGATC TCAAGTGGAG TCGCTTACCT 
CTGGCCTTTG CCTACAGAGA ACCCTATCTG TTTGTGACTC ACTTCAACTC ACTCGAAGTA 
ATTGAGATCC AGGCCCGCTC CTCTCTGGGG ACCCCCGCCC GAGCATATTT GGAAATCCCC 
AACCCACGCT ACCTGGGCCC TGCAATCTCC TCAGGAGCCA TCTACCTGGC GTCCTCATAT 
CAGGATAAGT TAAGGGTCAT CTGCTGCAAA GGAAACCTGG TGAAGGAGAC CGGCACCGAC 
CACCACCGTG GCCCATCCAC CTCCCGCAGC CCCAACAAGC GAGGCCCGCC GACGTACAAC 
GAGCACATCA CCAAGCGCGT GGCCTCAAGC CCGGCGCCTC CCGAAGGCCC CAGCCACCCT 
CGAGAGCCAA GCACACCCCA CCGCTACCGC GAGGGGCGGA CCGAGCTGCG CAGGGACAAG 
TCTCCGGGCC GCCCCCTGGA GCGCGAGAAG TCCCCCGGCC GGATGCTGAG CACGCGGAGG 
GAGCGCTCCC CCGGGCGGCT GTTTGAGGAC AGCAGCAGGG GCCGGCTGCC CGTGGGGGCC 
GTGAGGACCC CGCTGTCCCA GGTCAACAAG GTCTGGGACC AGTCTTCAGT ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024833511.1
CDS	173..5086
Translation	MEKKLLIKSKELQDSQDKCHKMEQEMTRLHRRVSEVEAVLSQKEVELKASETQRSLLEQDLATYITECSSLKRSLEQARMEVSQEDDKALQLLHDIREQSRKLQEIKEQEYQAQVEEMRLMMNQLEEDLVSARRRSDLYESELRESRLAAEEFKRKATECQHKLMKAKDQGKPEVGEYAKLEKINAEQQLKIQELQEKLEKAVKASTEATELLQNIRQAKERAERELEKLHNREDSSEGIKKKLVEAEERRHSLENKVKRLETMERRENRLKDDIQTKSQQIQQMADKILELEEKHREAQVSAQHLEVHLKQKEQHYEEKIKVLDNQIKKDLADKETLENLMQRHEEEAHEKGKILSEQKAMINAMDSKIRSLEQRIVELSEANKLAANSSLFTQRNMKAQEEMISELRQQKFYLETQAGKLEAQNRKLEEQLEKISHQDHSDKNRLLELETRLREVSLEHEEQKLELKRQLTELQLSLQERESQLTALQAARAALESQLRQAKTELEETTAEAEEEIQALTAHRDEIQRKFDALRNSCTVITDLEEQLNQLTEDNAELNNQNFYLSKQLDEASGANDEIVQLRSEVDHLRREITEREMQLTSQKQTMEALKTTCTMLEEQVMDLEALNDELLEKERQWEAWRSVLGDEKSQFECRVRELQRMLDTEKQSRARADQRITESRQVVELAVKEHKAEILALQQALKEQKLKAESLSDKLNDLEKKHAMLEMNARSLQQKLETERELKQRLLEEQAKLQQQMDLQKNHIFRLTQGLQEALDRADLLKTERSDLEYQLENIQVLYSHEKVKMEGTISQQTKLIDFLQAKMDQPAKKKKGLFSRRKEDPALPTQVPLQYNELKVALEKEKARCAELEEALQKTRIELRSAREEAAHRKAADHPHPSTPATARQQIAMSAIVRSPEHQPSALSLLAPPSSRRKESSTPEEFSRRLKERMHHNIPHRFNVGLNMRATKCAVCLDTVHFGRQASKCLECQVMCHPKCSTCLPATCGLPAEYATHFTEAFCRDKMNSPGLQSKEPSSSLHLEGWMKVPRNNKRGQQGWDRKYIVLEGSKVLIYDNEAREAGQRPVEEFELCLPDGDVSIHGAVGASELANTAKADVPYVLKMESHPHTTCWPGRTLYLLAPSFPDKQRWVTALESVVAGGRVSREKAEADAGRDCTSYEPLPAWAQKLLGNSLLKLEGDDRLDMNCTLPFSDQVVLVGTEEGLYALNVLKNSLTHVPGIGAVFQIYIIKDLEKLLMIAGEERALCLVDVKKVKQSLAQSHLPAQPDISPNIFEAVKGCHLFAAGKIESGLCICAAMPSKVVILRYNENLSKYCIRKEIETSEPCSCIHFTNYSILIGTNKFYEIDMKQYTLEEFLDKNDHSLAPAVFASSSNSFPVSIMQVNGAGQREEFLLCFHEFGVFVDSYGRRSRTDDLKWSRLPLAFAYREPYLFVTHFNSLEVIEIQARSSLGTPARAYLEIPNPRYLGPAISSGAIYLASSYQDKLRVICCKGNLVKETGTDHHRGPSTSRSPNKRGPPTYNEHITKRVASSPAPPEGPSHPREPSTPHRYREGRTELRRDKSPGRPLEREKSPGRMLSTRRERSPGRLFEDSSRGRLPVGAVRTPLSQVNKVWDQSSV

Target ORF information:

RefSeq Version	XM_024977743.1
Organism	Bos taurus(cattle)
Definition	Bos taurus citron rho-interacting serine/threonine kinase (CIT), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024977743.1

ORF Insert Sequence:

ATGGAAAAGA AACTCCTTAT CAAAAGCAAA GAGCTGCAGG ACTCCCAGGA CAAGTGTCAC 
AAGATGGAGC AGGAAATGAC CCGGTTACAT CGGAGAGTGT CAGAGGTGGA GGCTGTGCTT 
AGTCAGAAGG AGGTGGAGCT GAAGGCCTCT GAGACTCAGA GATCCCTCCT GGAGCAGGAC 
CTTGCTACCT ACATCACAGA ATGCAGTAGC TTAAAGCGAA GTTTGGAGCA AGCACGGATG 
GAGGTGTCCC AAGAGGACGA CAAAGCACTG CAGCTCCTCC ATGATATCAG AGAGCAGAGC 
CGGAAGCTCC AAGAAATCAA AGAGCAGGAG TACCAGGCTC AAGTGGAAGA AATGAGGTTG 
ATGATGAATC AGTTGGAAGA GGACCTGGTT TCGGCGAGAA GGCGGAGTGA CCTCTATGAA 
TCGGAGCTCA GAGAGTCTCG GCTGGCTGCT GAAGAGTTTA AGCGGAAAGC AACAGAATGT 
CAGCATAAAC TGATGAAGGC TAAAGATCAA GGGAAGCCTG AAGTGGGAGA ATATGCCAAA 
CTGGAGAAGA TCAATGCTGA GCAGCAGCTC AAAATTCAGG AGCTCCAAGA GAAGCTAGAA 
AAGGCTGTGA AAGCTAGCAC AGAGGCCACC GAGCTGCTGC AGAACATCCG TCAGGCGAAG 
GAGCGGGCCG AGCGTGAGCT GGAGAAGCTG CACAACCGTG AGGACTCCTC CGAGGGCATC 
AAGAAGAAGC TGGTGGAGGC CGAGGAACGC CGCCACTCTC TGGAGAACAA GGTAAAGAGG 
CTAGAGACCA TGGAGCGTAG AGAAAACAGA CTGAAGGATG ACATCCAGAC AAAGTCCCAA 
CAGATCCAGC AGATGGCTGA TAAAATTCTG GAGCTGGAGG AGAAGCACAG AGAAGCCCAG 
GTCTCAGCGC AGCATCTGGA GGTGCACCTG AAACAGAAAG AGCAGCACTA TGAAGAAAAA 
ATTAAAGTGT TGGACAATCA GATAAAGAAA GACCTAGCAG ACAAGGAGAC ACTGGAGAAC 
CTGATGCAAA GACACGAAGA GGAGGCCCAT GAGAAAGGCA AAATTCTCAG CGAGCAGAAG 
GCGATGATCA ATGCTATGGA TTCCAAGATC AGATCCCTGG AACAAAGGAT AGTGGAATTG 
TCGGAAGCCA ATAAACTTGC GGCCAACAGC AGTCTCTTTA CCCAGAGGAA CATGAAGGCC 
CAGGAAGAGA TGATTTCAGA ACTCAGGCAA CAGAAGTTTT ACCTGGAGAC GCAAGCTGGG 
AAATTGGAGG CCCAGAACCG GAAGCTGGAG GAGCAGCTGG AAAAAATAAG CCACCAAGAC 
CACAGTGACA AGAACCGTCT GCTGGAGCTG GAGACCAGGC TAAGGGAGGT CAGTCTAGAA 
CATGAAGAGC AGAAACTGGA GCTCAAACGC CAGCTCACAG AGCTGCAGCT CTCCCTGCAG 
GAGCGTGAAT CCCAGCTGAC AGCCCTGCAG GCAGCACGGG CGGCCCTGGA GAGCCAGCTC 
CGCCAGGCCA AGACGGAGCT GGAAGAGACG ACAGCAGAAG CAGAAGAAGA GATCCAGGCG 
CTTACGGCAC ATAGAGATGA AATCCAGCGC AAATTCGATG CCCTTCGTAA CAGCTGTACT 
GTGATCACGG ACTTGGAGGA GCAGCTAAAC CAGCTGACGG AGGACAATGC CGAGCTCAAC 
AACCAAAACT TCTACTTGTC TAAACAACTC GATGAGGCCT CTGGTGCCAA CGACGAGATT 
GTGCAGCTGC GAAGTGAAGT GGACCATCTC CGCCGCGAGA TCACTGAGAG GGAGATGCAG 
CTCACTAGCC AGAAGCAAAC GATGGAGGCG CTGAAGACCA CGTGCACAAT GCTGGAGGAG 
CAGGTCATGG ACCTGGAGGC CCTGAACGAC GAGCTGCTGG AGAAGGAGCG GCAGTGGGAG 
GCCTGGAGGA GTGTCCTTGG TGACGAGAAG TCCCAGTTTG AGTGTCGGGT TCGAGAGTTG 
CAGAGGATGC TGGACACCGA AAAGCAGAGC AGGGCGAGGG CCGACCAGAG GATCACTGAG 
TCGCGCCAGG TGGTCGAGCT GGCAGTAAAG GAGCACAAGG CCGAGATCCT GGCCTTGCAG 
CAGGCTCTCA AGGAGCAGAA GCTGAAGGCC GAGAGCCTCT CCGACAAGCT CAATGATCTG 
GAGAAGAAGC ACGCCATGCT TGAAATGAAT GCCCGAAGTT TACAACAGAA GCTGGAGACA 
GAACGAGAGC TCAAACAAAG GCTTCTGGAA GAGCAAGCCA AGTTACAGCA GCAGATGGAC 
CTGCAGAAGA ATCATATTTT CCGTCTGACT CAAGGGCTGC AAGAAGCTCT GGATCGGGCT 
GATCTGCTGA AGACAGAAAG GAGTGACCTG GAATATCAGC TGGAAAACAT TCAGGTTCTC 
TATTCTCATG AGAAGGTGAA GATGGAAGGC ACTATTTCCC AGCAAACCAA ACTCATCGAT 
TTTCTGCAAG CCAAAATGGA CCAGCCTGCC AAAAAGAAAA AGGGTTTATT TAGTCGACGG 
AAAGAGGACC CTGCTTTGCC CACACAGGTT CCTCTGCAGT ACAATGAGCT GAAGGTGGCT 
CTGGAGAAGG AGAAAGCTCG CTGCGCGGAG CTCGAGGAAG CCCTGCAGAA GACCCGCATC 
GAGCTCCGCT CCGCCCGGGA GGAAGCTGCC CACCGGAAAG CTGCAGACCA CCCACACCCA 
TCTACGCCGG CTACCGCCAG GCAGCAGATC GCCATGTCCG CCATTGTGCG GTCACCCGAG 
CACCAGCCCA GCGCCCTGAG CCTGCTCGCC CCGCCGTCCA GCCGCAGGAA GGAGTCTTCC 
ACTCCAGAGG AATTCAGCCG GCGTCTTAAG GAGCGCATGC ACCACAACAT CCCTCACCGG 
TTCAACGTGG GACTGAACAT GCGAGCCACG AAGTGCGCCG TGTGTCTGGA TACCGTGCAC 
TTCGGGCGCC AGGCATCCAA GTGTCTCGAA TGTCAGGTCA TGTGCCACCC CAAGTGCTCC 
ACATGCTTGC CAGCCACCTG TGGCCTGCCA GCCGAATACG CCACACACTT CACCGAGGCC 
TTCTGCCGCG ACAAAATGAA CTCCCCGGGT CTGCAGTCCA AGGAGCCCAG CAGCAGCCTG 
CACCTGGAAG GGTGGATGAA GGTGCCCAGG AATAACAAGC GAGGACAGCA AGGCTGGGAC 
AGGAAGTACA TCGTTCTGGA GGGCTCCAAA GTCCTCATTT ATGACAACGA AGCCAGAGAA 
GCTGGACAGA GGCCGGTGGA AGAATTTGAG CTGTGCCTTC CCGACGGGGA CGTGTCTATT 
CATGGCGCCG TTGGTGCTTC TGAACTTGCA AACACAGCCA AAGCAGATGT CCCCTATGTC 
CTGAAGATGG AGTCTCACCC CCATACCACC TGCTGGCCTG GGAGGACCCT CTACTTGCTG 
GCTCCCAGCT TTCCCGACAA GCAGCGCTGG GTCACCGCCT TAGAGTCAGT TGTGGCAGGT 
GGGAGAGTGT CTAGGGAAAA GGCGGAAGCC GACGCTGGCC GTGACTGTAC TTCCTACGAG 
CCTCTGCCCG CGTGGGCTCA GAAATTGCTC GGAAACTCCC TGTTGAAACT GGAAGGTGAT 
GACCGTCTGG ACATGAACTG CACGCTGCCC TTCAGCGACC AGGTGGTGCT GGTGGGCACC 
GAGGAAGGGC TGTATGCGCT GAATGTCTTG AAGAACTCCC TCACCCACGT CCCAGGAATC 
GGAGCAGTCT TCCAGATTTA CATCATCAAG GACCTGGAGA AGCTCCTCAT GATCGCAGGA 
GAGGAGCGGG CCCTGTGTCT CGTGGATGTG AAGAAGGTGA AGCAGTCCCT CGCGCAGTCT 
CACCTCCCCG CCCAGCCAGA CATCTCGCCC AACATCTTTG AAGCGGTCAA GGGCTGCCAC 
TTGTTTGCTG CTGGCAAGAT TGAGAGCGGG CTCTGCATCT GTGCAGCCAT GCCCAGCAAA 
GTTGTCATTC TCCGCTACAA TGAAAACCTC AGCAAGTACT GCATTCGCAA AGAGATCGAG 
ACCTCGGAGC CCTGCAGCTG TATCCACTTC ACCAACTACA GTATCCTCAT CGGAACCAAT 
AAATTCTACG AAATCGACAT GAAGCAGTAC ACGCTAGAGG AATTCCTGGA TAAGAACGAC 
CATTCCTTGG CGCCTGCCGT GTTTGCCTCC TCTTCCAACA GCTTCCCCGT GTCCATCATG 
CAGGTGAACG GCGCGGGGCA GCGGGAGGAG TTCCTGCTCT GTTTCCACGA ATTCGGGGTG 
TTCGTGGATT CTTATGGAAG ACGCAGCCGC ACAGATGATC TCAAGTGGAG TCGCTTACCT 
CTGGCCTTTG CCTACAGAGA ACCCTATCTG TTTGTGACTC ACTTCAACTC ACTCGAAGTA 
ATTGAGATCC AGGCCCGCTC CTCTCTGGGG ACCCCCGCCC GAGCATATTT GGAAATCCCC 
AACCCACGCT ACCTGGGCCC TGCAATCTCC TCAGGAGCCA TCTACCTGGC GTCCTCATAT 
CAGGATAAGT TAAGGGTCAT CTGCTGCAAA GGAAACCTGG TGAAGGAGAC CGGCACCGAC 
CACCACCGTG GCCCATCCAC CTCCCGCAGC CCCAACAAGC GAGGCCCGCC GACGTACAAC 
GAGCACATCA CCAAGCGCGT GGCCTCAAGC CCGGCGCCTC CCGAAGGCCC CAGCCACCCT 
CGAGAGCCAA GCACACCCCA CCGCTACCGC GAGGGGCGGA CCGAGCTGCG CAGGGACAAG 
TCTCCGGGCC GCCCCCTGGA GCGCGAGAAG TCCCCCGGCC GGATGCTGAG CACGCGGAGG 
GAGCGCTCCC CCGGGCGGCT GTTTGAGGAC AGCAGCAGGG GCCGGCTGCC CGTGGGGGCC 
GTGAGGACCC CGCTGTCCCA GGTCAACAAG GTCTGGGACC AGTCTTCAGT ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CIT ( XM_024977742.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024833510.1 Bos taurus citron rho-interacting serine/threonine kinase (CIT), transcript variant X10, mRNA.

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CloneID	OBa224630
Clone ID Related Accession (Same CDS sequence)	XM_024977743.1 , XM_024977742.1
Accession Version	XM_024977742.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4914bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	citron Rho-interacting kinase isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAAGA AACTCCTTAT CAAAAGCAAA GAGCTGCAGG ACTCCCAGGA CAAGTGTCAC 
AAGATGGAGC AGGAAATGAC CCGGTTACAT CGGAGAGTGT CAGAGGTGGA GGCTGTGCTT 
AGTCAGAAGG AGGTGGAGCT GAAGGCCTCT GAGACTCAGA GATCCCTCCT GGAGCAGGAC 
CTTGCTACCT ACATCACAGA ATGCAGTAGC TTAAAGCGAA GTTTGGAGCA AGCACGGATG 
GAGGTGTCCC AAGAGGACGA CAAAGCACTG CAGCTCCTCC ATGATATCAG AGAGCAGAGC 
CGGAAGCTCC AAGAAATCAA AGAGCAGGAG TACCAGGCTC AAGTGGAAGA AATGAGGTTG 
ATGATGAATC AGTTGGAAGA GGACCTGGTT TCGGCGAGAA GGCGGAGTGA CCTCTATGAA 
TCGGAGCTCA GAGAGTCTCG GCTGGCTGCT GAAGAGTTTA AGCGGAAAGC AACAGAATGT 
CAGCATAAAC TGATGAAGGC TAAAGATCAA GGGAAGCCTG AAGTGGGAGA ATATGCCAAA 
CTGGAGAAGA TCAATGCTGA GCAGCAGCTC AAAATTCAGG AGCTCCAAGA GAAGCTAGAA 
AAGGCTGTGA AAGCTAGCAC AGAGGCCACC GAGCTGCTGC AGAACATCCG TCAGGCGAAG 
GAGCGGGCCG AGCGTGAGCT GGAGAAGCTG CACAACCGTG AGGACTCCTC CGAGGGCATC 
AAGAAGAAGC TGGTGGAGGC CGAGGAACGC CGCCACTCTC TGGAGAACAA GGTAAAGAGG 
CTAGAGACCA TGGAGCGTAG AGAAAACAGA CTGAAGGATG ACATCCAGAC AAAGTCCCAA 
CAGATCCAGC AGATGGCTGA TAAAATTCTG GAGCTGGAGG AGAAGCACAG AGAAGCCCAG 
GTCTCAGCGC AGCATCTGGA GGTGCACCTG AAACAGAAAG AGCAGCACTA TGAAGAAAAA 
ATTAAAGTGT TGGACAATCA GATAAAGAAA GACCTAGCAG ACAAGGAGAC ACTGGAGAAC 
CTGATGCAAA GACACGAAGA GGAGGCCCAT GAGAAAGGCA AAATTCTCAG CGAGCAGAAG 
GCGATGATCA ATGCTATGGA TTCCAAGATC AGATCCCTGG AACAAAGGAT AGTGGAATTG 
TCGGAAGCCA ATAAACTTGC GGCCAACAGC AGTCTCTTTA CCCAGAGGAA CATGAAGGCC 
CAGGAAGAGA TGATTTCAGA ACTCAGGCAA CAGAAGTTTT ACCTGGAGAC GCAAGCTGGG 
AAATTGGAGG CCCAGAACCG GAAGCTGGAG GAGCAGCTGG AAAAAATAAG CCACCAAGAC 
CACAGTGACA AGAACCGTCT GCTGGAGCTG GAGACCAGGC TAAGGGAGGT CAGTCTAGAA 
CATGAAGAGC AGAAACTGGA GCTCAAACGC CAGCTCACAG AGCTGCAGCT CTCCCTGCAG 
GAGCGTGAAT CCCAGCTGAC AGCCCTGCAG GCAGCACGGG CGGCCCTGGA GAGCCAGCTC 
CGCCAGGCCA AGACGGAGCT GGAAGAGACG ACAGCAGAAG CAGAAGAAGA GATCCAGGCG 
CTTACGGCAC ATAGAGATGA AATCCAGCGC AAATTCGATG CCCTTCGTAA CAGCTGTACT 
GTGATCACGG ACTTGGAGGA GCAGCTAAAC CAGCTGACGG AGGACAATGC CGAGCTCAAC 
AACCAAAACT TCTACTTGTC TAAACAACTC GATGAGGCCT CTGGTGCCAA CGACGAGATT 
GTGCAGCTGC GAAGTGAAGT GGACCATCTC CGCCGCGAGA TCACTGAGAG GGAGATGCAG 
CTCACTAGCC AGAAGCAAAC GATGGAGGCG CTGAAGACCA CGTGCACAAT GCTGGAGGAG 
CAGGTCATGG ACCTGGAGGC CCTGAACGAC GAGCTGCTGG AGAAGGAGCG GCAGTGGGAG 
GCCTGGAGGA GTGTCCTTGG TGACGAGAAG TCCCAGTTTG AGTGTCGGGT TCGAGAGTTG 
CAGAGGATGC TGGACACCGA AAAGCAGAGC AGGGCGAGGG CCGACCAGAG GATCACTGAG 
TCGCGCCAGG TGGTCGAGCT GGCAGTAAAG GAGCACAAGG CCGAGATCCT GGCCTTGCAG 
CAGGCTCTCA AGGAGCAGAA GCTGAAGGCC GAGAGCCTCT CCGACAAGCT CAATGATCTG 
GAGAAGAAGC ACGCCATGCT TGAAATGAAT GCCCGAAGTT TACAACAGAA GCTGGAGACA 
GAACGAGAGC TCAAACAAAG GCTTCTGGAA GAGCAAGCCA AGTTACAGCA GCAGATGGAC 
CTGCAGAAGA ATCATATTTT CCGTCTGACT CAAGGGCTGC AAGAAGCTCT GGATCGGGCT 
GATCTGCTGA AGACAGAAAG GAGTGACCTG GAATATCAGC TGGAAAACAT TCAGGTTCTC 
TATTCTCATG AGAAGGTGAA GATGGAAGGC ACTATTTCCC AGCAAACCAA ACTCATCGAT 
TTTCTGCAAG CCAAAATGGA CCAGCCTGCC AAAAAGAAAA AGGGTTTATT TAGTCGACGG 
AAAGAGGACC CTGCTTTGCC CACACAGGTT CCTCTGCAGT ACAATGAGCT GAAGGTGGCT 
CTGGAGAAGG AGAAAGCTCG CTGCGCGGAG CTCGAGGAAG CCCTGCAGAA GACCCGCATC 
GAGCTCCGCT CCGCCCGGGA GGAAGCTGCC CACCGGAAAG CTGCAGACCA CCCACACCCA 
TCTACGCCGG CTACCGCCAG GCAGCAGATC GCCATGTCCG CCATTGTGCG GTCACCCGAG 
CACCAGCCCA GCGCCCTGAG CCTGCTCGCC CCGCCGTCCA GCCGCAGGAA GGAGTCTTCC 
ACTCCAGAGG AATTCAGCCG GCGTCTTAAG GAGCGCATGC ACCACAACAT CCCTCACCGG 
TTCAACGTGG GACTGAACAT GCGAGCCACG AAGTGCGCCG TGTGTCTGGA TACCGTGCAC 
TTCGGGCGCC AGGCATCCAA GTGTCTCGAA TGTCAGGTCA TGTGCCACCC CAAGTGCTCC 
ACATGCTTGC CAGCCACCTG TGGCCTGCCA GCCGAATACG CCACACACTT CACCGAGGCC 
TTCTGCCGCG ACAAAATGAA CTCCCCGGGT CTGCAGTCCA AGGAGCCCAG CAGCAGCCTG 
CACCTGGAAG GGTGGATGAA GGTGCCCAGG AATAACAAGC GAGGACAGCA AGGCTGGGAC 
AGGAAGTACA TCGTTCTGGA GGGCTCCAAA GTCCTCATTT ATGACAACGA AGCCAGAGAA 
GCTGGACAGA GGCCGGTGGA AGAATTTGAG CTGTGCCTTC CCGACGGGGA CGTGTCTATT 
CATGGCGCCG TTGGTGCTTC TGAACTTGCA AACACAGCCA AAGCAGATGT CCCCTATGTC 
CTGAAGATGG AGTCTCACCC CCATACCACC TGCTGGCCTG GGAGGACCCT CTACTTGCTG 
GCTCCCAGCT TTCCCGACAA GCAGCGCTGG GTCACCGCCT TAGAGTCAGT TGTGGCAGGT 
GGGAGAGTGT CTAGGGAAAA GGCGGAAGCC GACGCTGGCC GTGACTGTAC TTCCTACGAG 
CCTCTGCCCG CGTGGGCTCA GAAATTGCTC GGAAACTCCC TGTTGAAACT GGAAGGTGAT 
GACCGTCTGG ACATGAACTG CACGCTGCCC TTCAGCGACC AGGTGGTGCT GGTGGGCACC 
GAGGAAGGGC TGTATGCGCT GAATGTCTTG AAGAACTCCC TCACCCACGT CCCAGGAATC 
GGAGCAGTCT TCCAGATTTA CATCATCAAG GACCTGGAGA AGCTCCTCAT GATCGCAGGA 
GAGGAGCGGG CCCTGTGTCT CGTGGATGTG AAGAAGGTGA AGCAGTCCCT CGCGCAGTCT 
CACCTCCCCG CCCAGCCAGA CATCTCGCCC AACATCTTTG AAGCGGTCAA GGGCTGCCAC 
TTGTTTGCTG CTGGCAAGAT TGAGAGCGGG CTCTGCATCT GTGCAGCCAT GCCCAGCAAA 
GTTGTCATTC TCCGCTACAA TGAAAACCTC AGCAAGTACT GCATTCGCAA AGAGATCGAG 
ACCTCGGAGC CCTGCAGCTG TATCCACTTC ACCAACTACA GTATCCTCAT CGGAACCAAT 
AAATTCTACG AAATCGACAT GAAGCAGTAC ACGCTAGAGG AATTCCTGGA TAAGAACGAC 
CATTCCTTGG CGCCTGCCGT GTTTGCCTCC TCTTCCAACA GCTTCCCCGT GTCCATCATG 
CAGGTGAACG GCGCGGGGCA GCGGGAGGAG TTCCTGCTCT GTTTCCACGA ATTCGGGGTG 
TTCGTGGATT CTTATGGAAG ACGCAGCCGC ACAGATGATC TCAAGTGGAG TCGCTTACCT 
CTGGCCTTTG CCTACAGAGA ACCCTATCTG TTTGTGACTC ACTTCAACTC ACTCGAAGTA 
ATTGAGATCC AGGCCCGCTC CTCTCTGGGG ACCCCCGCCC GAGCATATTT GGAAATCCCC 
AACCCACGCT ACCTGGGCCC TGCAATCTCC TCAGGAGCCA TCTACCTGGC GTCCTCATAT 
CAGGATAAGT TAAGGGTCAT CTGCTGCAAA GGAAACCTGG TGAAGGAGAC CGGCACCGAC 
CACCACCGTG GCCCATCCAC CTCCCGCAGC CCCAACAAGC GAGGCCCGCC GACGTACAAC 
GAGCACATCA CCAAGCGCGT GGCCTCAAGC CCGGCGCCTC CCGAAGGCCC CAGCCACCCT 
CGAGAGCCAA GCACACCCCA CCGCTACCGC GAGGGGCGGA CCGAGCTGCG CAGGGACAAG 
TCTCCGGGCC GCCCCCTGGA GCGCGAGAAG TCCCCCGGCC GGATGCTGAG CACGCGGAGG 
GAGCGCTCCC CCGGGCGGCT GTTTGAGGAC AGCAGCAGGG GCCGGCTGCC CGTGGGGGCC 
GTGAGGACCC CGCTGTCCCA GGTCAACAAG GTCTGGGACC AGTCTTCAGT ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024833510.1
CDS	542..5455
Translation	MEKKLLIKSKELQDSQDKCHKMEQEMTRLHRRVSEVEAVLSQKEVELKASETQRSLLEQDLATYITECSSLKRSLEQARMEVSQEDDKALQLLHDIREQSRKLQEIKEQEYQAQVEEMRLMMNQLEEDLVSARRRSDLYESELRESRLAAEEFKRKATECQHKLMKAKDQGKPEVGEYAKLEKINAEQQLKIQELQEKLEKAVKASTEATELLQNIRQAKERAERELEKLHNREDSSEGIKKKLVEAEERRHSLENKVKRLETMERRENRLKDDIQTKSQQIQQMADKILELEEKHREAQVSAQHLEVHLKQKEQHYEEKIKVLDNQIKKDLADKETLENLMQRHEEEAHEKGKILSEQKAMINAMDSKIRSLEQRIVELSEANKLAANSSLFTQRNMKAQEEMISELRQQKFYLETQAGKLEAQNRKLEEQLEKISHQDHSDKNRLLELETRLREVSLEHEEQKLELKRQLTELQLSLQERESQLTALQAARAALESQLRQAKTELEETTAEAEEEIQALTAHRDEIQRKFDALRNSCTVITDLEEQLNQLTEDNAELNNQNFYLSKQLDEASGANDEIVQLRSEVDHLRREITEREMQLTSQKQTMEALKTTCTMLEEQVMDLEALNDELLEKERQWEAWRSVLGDEKSQFECRVRELQRMLDTEKQSRARADQRITESRQVVELAVKEHKAEILALQQALKEQKLKAESLSDKLNDLEKKHAMLEMNARSLQQKLETERELKQRLLEEQAKLQQQMDLQKNHIFRLTQGLQEALDRADLLKTERSDLEYQLENIQVLYSHEKVKMEGTISQQTKLIDFLQAKMDQPAKKKKGLFSRRKEDPALPTQVPLQYNELKVALEKEKARCAELEEALQKTRIELRSAREEAAHRKAADHPHPSTPATARQQIAMSAIVRSPEHQPSALSLLAPPSSRRKESSTPEEFSRRLKERMHHNIPHRFNVGLNMRATKCAVCLDTVHFGRQASKCLECQVMCHPKCSTCLPATCGLPAEYATHFTEAFCRDKMNSPGLQSKEPSSSLHLEGWMKVPRNNKRGQQGWDRKYIVLEGSKVLIYDNEAREAGQRPVEEFELCLPDGDVSIHGAVGASELANTAKADVPYVLKMESHPHTTCWPGRTLYLLAPSFPDKQRWVTALESVVAGGRVSREKAEADAGRDCTSYEPLPAWAQKLLGNSLLKLEGDDRLDMNCTLPFSDQVVLVGTEEGLYALNVLKNSLTHVPGIGAVFQIYIIKDLEKLLMIAGEERALCLVDVKKVKQSLAQSHLPAQPDISPNIFEAVKGCHLFAAGKIESGLCICAAMPSKVVILRYNENLSKYCIRKEIETSEPCSCIHFTNYSILIGTNKFYEIDMKQYTLEEFLDKNDHSLAPAVFASSSNSFPVSIMQVNGAGQREEFLLCFHEFGVFVDSYGRRSRTDDLKWSRLPLAFAYREPYLFVTHFNSLEVIEIQARSSLGTPARAYLEIPNPRYLGPAISSGAIYLASSYQDKLRVICCKGNLVKETGTDHHRGPSTSRSPNKRGPPTYNEHITKRVASSPAPPEGPSHPREPSTPHRYREGRTELRRDKSPGRPLEREKSPGRMLSTRRERSPGRLFEDSSRGRLPVGAVRTPLSQVNKVWDQSSV

Target ORF information:

RefSeq Version	XM_024977742.1
Organism	Bos taurus(cattle)
Definition	Bos taurus citron rho-interacting serine/threonine kinase (CIT), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024977742.1

ORF Insert Sequence:

ATGGAAAAGA AACTCCTTAT CAAAAGCAAA GAGCTGCAGG ACTCCCAGGA CAAGTGTCAC 
AAGATGGAGC AGGAAATGAC CCGGTTACAT CGGAGAGTGT CAGAGGTGGA GGCTGTGCTT 
AGTCAGAAGG AGGTGGAGCT GAAGGCCTCT GAGACTCAGA GATCCCTCCT GGAGCAGGAC 
CTTGCTACCT ACATCACAGA ATGCAGTAGC TTAAAGCGAA GTTTGGAGCA AGCACGGATG 
GAGGTGTCCC AAGAGGACGA CAAAGCACTG CAGCTCCTCC ATGATATCAG AGAGCAGAGC 
CGGAAGCTCC AAGAAATCAA AGAGCAGGAG TACCAGGCTC AAGTGGAAGA AATGAGGTTG 
ATGATGAATC AGTTGGAAGA GGACCTGGTT TCGGCGAGAA GGCGGAGTGA CCTCTATGAA 
TCGGAGCTCA GAGAGTCTCG GCTGGCTGCT GAAGAGTTTA AGCGGAAAGC AACAGAATGT 
CAGCATAAAC TGATGAAGGC TAAAGATCAA GGGAAGCCTG AAGTGGGAGA ATATGCCAAA 
CTGGAGAAGA TCAATGCTGA GCAGCAGCTC AAAATTCAGG AGCTCCAAGA GAAGCTAGAA 
AAGGCTGTGA AAGCTAGCAC AGAGGCCACC GAGCTGCTGC AGAACATCCG TCAGGCGAAG 
GAGCGGGCCG AGCGTGAGCT GGAGAAGCTG CACAACCGTG AGGACTCCTC CGAGGGCATC 
AAGAAGAAGC TGGTGGAGGC CGAGGAACGC CGCCACTCTC TGGAGAACAA GGTAAAGAGG 
CTAGAGACCA TGGAGCGTAG AGAAAACAGA CTGAAGGATG ACATCCAGAC AAAGTCCCAA 
CAGATCCAGC AGATGGCTGA TAAAATTCTG GAGCTGGAGG AGAAGCACAG AGAAGCCCAG 
GTCTCAGCGC AGCATCTGGA GGTGCACCTG AAACAGAAAG AGCAGCACTA TGAAGAAAAA 
ATTAAAGTGT TGGACAATCA GATAAAGAAA GACCTAGCAG ACAAGGAGAC ACTGGAGAAC 
CTGATGCAAA GACACGAAGA GGAGGCCCAT GAGAAAGGCA AAATTCTCAG CGAGCAGAAG 
GCGATGATCA ATGCTATGGA TTCCAAGATC AGATCCCTGG AACAAAGGAT AGTGGAATTG 
TCGGAAGCCA ATAAACTTGC GGCCAACAGC AGTCTCTTTA CCCAGAGGAA CATGAAGGCC 
CAGGAAGAGA TGATTTCAGA ACTCAGGCAA CAGAAGTTTT ACCTGGAGAC GCAAGCTGGG 
AAATTGGAGG CCCAGAACCG GAAGCTGGAG GAGCAGCTGG AAAAAATAAG CCACCAAGAC 
CACAGTGACA AGAACCGTCT GCTGGAGCTG GAGACCAGGC TAAGGGAGGT CAGTCTAGAA 
CATGAAGAGC AGAAACTGGA GCTCAAACGC CAGCTCACAG AGCTGCAGCT CTCCCTGCAG 
GAGCGTGAAT CCCAGCTGAC AGCCCTGCAG GCAGCACGGG CGGCCCTGGA GAGCCAGCTC 
CGCCAGGCCA AGACGGAGCT GGAAGAGACG ACAGCAGAAG CAGAAGAAGA GATCCAGGCG 
CTTACGGCAC ATAGAGATGA AATCCAGCGC AAATTCGATG CCCTTCGTAA CAGCTGTACT 
GTGATCACGG ACTTGGAGGA GCAGCTAAAC CAGCTGACGG AGGACAATGC CGAGCTCAAC 
AACCAAAACT TCTACTTGTC TAAACAACTC GATGAGGCCT CTGGTGCCAA CGACGAGATT 
GTGCAGCTGC GAAGTGAAGT GGACCATCTC CGCCGCGAGA TCACTGAGAG GGAGATGCAG 
CTCACTAGCC AGAAGCAAAC GATGGAGGCG CTGAAGACCA CGTGCACAAT GCTGGAGGAG 
CAGGTCATGG ACCTGGAGGC CCTGAACGAC GAGCTGCTGG AGAAGGAGCG GCAGTGGGAG 
GCCTGGAGGA GTGTCCTTGG TGACGAGAAG TCCCAGTTTG AGTGTCGGGT TCGAGAGTTG 
CAGAGGATGC TGGACACCGA AAAGCAGAGC AGGGCGAGGG CCGACCAGAG GATCACTGAG 
TCGCGCCAGG TGGTCGAGCT GGCAGTAAAG GAGCACAAGG CCGAGATCCT GGCCTTGCAG 
CAGGCTCTCA AGGAGCAGAA GCTGAAGGCC GAGAGCCTCT CCGACAAGCT CAATGATCTG 
GAGAAGAAGC ACGCCATGCT TGAAATGAAT GCCCGAAGTT TACAACAGAA GCTGGAGACA 
GAACGAGAGC TCAAACAAAG GCTTCTGGAA GAGCAAGCCA AGTTACAGCA GCAGATGGAC 
CTGCAGAAGA ATCATATTTT CCGTCTGACT CAAGGGCTGC AAGAAGCTCT GGATCGGGCT 
GATCTGCTGA AGACAGAAAG GAGTGACCTG GAATATCAGC TGGAAAACAT TCAGGTTCTC 
TATTCTCATG AGAAGGTGAA GATGGAAGGC ACTATTTCCC AGCAAACCAA ACTCATCGAT 
TTTCTGCAAG CCAAAATGGA CCAGCCTGCC AAAAAGAAAA AGGGTTTATT TAGTCGACGG 
AAAGAGGACC CTGCTTTGCC CACACAGGTT CCTCTGCAGT ACAATGAGCT GAAGGTGGCT 
CTGGAGAAGG AGAAAGCTCG CTGCGCGGAG CTCGAGGAAG CCCTGCAGAA GACCCGCATC 
GAGCTCCGCT CCGCCCGGGA GGAAGCTGCC CACCGGAAAG CTGCAGACCA CCCACACCCA 
TCTACGCCGG CTACCGCCAG GCAGCAGATC GCCATGTCCG CCATTGTGCG GTCACCCGAG 
CACCAGCCCA GCGCCCTGAG CCTGCTCGCC CCGCCGTCCA GCCGCAGGAA GGAGTCTTCC 
ACTCCAGAGG AATTCAGCCG GCGTCTTAAG GAGCGCATGC ACCACAACAT CCCTCACCGG 
TTCAACGTGG GACTGAACAT GCGAGCCACG AAGTGCGCCG TGTGTCTGGA TACCGTGCAC 
TTCGGGCGCC AGGCATCCAA GTGTCTCGAA TGTCAGGTCA TGTGCCACCC CAAGTGCTCC 
ACATGCTTGC CAGCCACCTG TGGCCTGCCA GCCGAATACG CCACACACTT CACCGAGGCC 
TTCTGCCGCG ACAAAATGAA CTCCCCGGGT CTGCAGTCCA AGGAGCCCAG CAGCAGCCTG 
CACCTGGAAG GGTGGATGAA GGTGCCCAGG AATAACAAGC GAGGACAGCA AGGCTGGGAC 
AGGAAGTACA TCGTTCTGGA GGGCTCCAAA GTCCTCATTT ATGACAACGA AGCCAGAGAA 
GCTGGACAGA GGCCGGTGGA AGAATTTGAG CTGTGCCTTC CCGACGGGGA CGTGTCTATT 
CATGGCGCCG TTGGTGCTTC TGAACTTGCA AACACAGCCA AAGCAGATGT CCCCTATGTC 
CTGAAGATGG AGTCTCACCC CCATACCACC TGCTGGCCTG GGAGGACCCT CTACTTGCTG 
GCTCCCAGCT TTCCCGACAA GCAGCGCTGG GTCACCGCCT TAGAGTCAGT TGTGGCAGGT 
GGGAGAGTGT CTAGGGAAAA GGCGGAAGCC GACGCTGGCC GTGACTGTAC TTCCTACGAG 
CCTCTGCCCG CGTGGGCTCA GAAATTGCTC GGAAACTCCC TGTTGAAACT GGAAGGTGAT 
GACCGTCTGG ACATGAACTG CACGCTGCCC TTCAGCGACC AGGTGGTGCT GGTGGGCACC 
GAGGAAGGGC TGTATGCGCT GAATGTCTTG AAGAACTCCC TCACCCACGT CCCAGGAATC 
GGAGCAGTCT TCCAGATTTA CATCATCAAG GACCTGGAGA AGCTCCTCAT GATCGCAGGA 
GAGGAGCGGG CCCTGTGTCT CGTGGATGTG AAGAAGGTGA AGCAGTCCCT CGCGCAGTCT 
CACCTCCCCG CCCAGCCAGA CATCTCGCCC AACATCTTTG AAGCGGTCAA GGGCTGCCAC 
TTGTTTGCTG CTGGCAAGAT TGAGAGCGGG CTCTGCATCT GTGCAGCCAT GCCCAGCAAA 
GTTGTCATTC TCCGCTACAA TGAAAACCTC AGCAAGTACT GCATTCGCAA AGAGATCGAG 
ACCTCGGAGC CCTGCAGCTG TATCCACTTC ACCAACTACA GTATCCTCAT CGGAACCAAT 
AAATTCTACG AAATCGACAT GAAGCAGTAC ACGCTAGAGG AATTCCTGGA TAAGAACGAC 
CATTCCTTGG CGCCTGCCGT GTTTGCCTCC TCTTCCAACA GCTTCCCCGT GTCCATCATG 
CAGGTGAACG GCGCGGGGCA GCGGGAGGAG TTCCTGCTCT GTTTCCACGA ATTCGGGGTG 
TTCGTGGATT CTTATGGAAG ACGCAGCCGC ACAGATGATC TCAAGTGGAG TCGCTTACCT 
CTGGCCTTTG CCTACAGAGA ACCCTATCTG TTTGTGACTC ACTTCAACTC ACTCGAAGTA 
ATTGAGATCC AGGCCCGCTC CTCTCTGGGG ACCCCCGCCC GAGCATATTT GGAAATCCCC 
AACCCACGCT ACCTGGGCCC TGCAATCTCC TCAGGAGCCA TCTACCTGGC GTCCTCATAT 
CAGGATAAGT TAAGGGTCAT CTGCTGCAAA GGAAACCTGG TGAAGGAGAC CGGCACCGAC 
CACCACCGTG GCCCATCCAC CTCCCGCAGC CCCAACAAGC GAGGCCCGCC GACGTACAAC 
GAGCACATCA CCAAGCGCGT GGCCTCAAGC CCGGCGCCTC CCGAAGGCCC CAGCCACCCT 
CGAGAGCCAA GCACACCCCA CCGCTACCGC GAGGGGCGGA CCGAGCTGCG CAGGGACAAG 
TCTCCGGGCC GCCCCCTGGA GCGCGAGAAG TCCCCCGGCC GGATGCTGAG CACGCGGAGG 
GAGCGCTCCC CCGGGCGGCT GTTTGAGGAC AGCAGCAGGG GCCGGCTGCC CGTGGGGGCC 
GTGAGGACCC CGCTGTCCCA GGTCAACAAG GTCTGGGACC AGTCTTCAGT ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CIT ( XM_024977744.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024833512.1 Bos taurus citron rho-interacting serine/threonine kinase (CIT), transcript variant X12, mRNA.

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CloneID	OBa224631
Clone ID Related Accession (Same CDS sequence)	XM_024977744.1
Accession Version	XM_024977744.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4854bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	citron Rho-interacting kinase isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGAGC AGGAAATGAC CCGGTTACAT CGGAGAGTGT CAGAGGTGGA GGCTGTGCTT 
AGTCAGAAGG AGGTGGAGCT GAAGGCCTCT GAGACTCAGA GATCCCTCCT GGAGCAGGAC 
CTTGCTACCT ACATCACAGA ATGCAGTAGC TTAAAGCGAA GTTTGGAGCA AGCACGGATG 
GAGGTGTCCC AAGAGGACGA CAAAGCACTG CAGCTCCTCC ATGATATCAG AGAGCAGAGC 
CGGAAGCTCC AAGAAATCAA AGAGCAGGAG TACCAGGCTC AAGTGGAAGA AATGAGGTTG 
ATGATGAATC AGTTGGAAGA GGACCTGGTT TCGGCGAGAA GGCGGAGTGA CCTCTATGAA 
TCGGAGCTCA GAGAGTCTCG GCTGGCTGCT GAAGAGTTTA AGCGGAAAGC AACAGAATGT 
CAGCATAAAC TGATGAAGGC TAAAGATCAA GGGAAGCCTG AAGTGGGAGA ATATGCCAAA 
CTGGAGAAGA TCAATGCTGA GCAGCAGCTC AAAATTCAGG AGCTCCAAGA GAAGCTAGAA 
AAGGCTGTGA AAGCTAGCAC AGAGGCCACC GAGCTGCTGC AGAACATCCG TCAGGCGAAG 
GAGCGGGCCG AGCGTGAGCT GGAGAAGCTG CACAACCGTG AGGACTCCTC CGAGGGCATC 
AAGAAGAAGC TGGTGGAGGC CGAGGAACGC CGCCACTCTC TGGAGAACAA GGTAAAGAGG 
CTAGAGACCA TGGAGCGTAG AGAAAACAGA CTGAAGGATG ACATCCAGAC AAAGTCCCAA 
CAGATCCAGC AGATGGCTGA TAAAATTCTG GAGCTGGAGG AGAAGCACAG AGAAGCCCAG 
GTCTCAGCGC AGCATCTGGA GGTGCACCTG AAACAGAAAG AGCAGCACTA TGAAGAAAAA 
ATTAAAGTGT TGGACAATCA GATAAAGAAA GACCTAGCAG ACAAGGAGAC ACTGGAGAAC 
CTGATGCAAA GACACGAAGA GGAGGCCCAT GAGAAAGGCA AAATTCTCAG CGAGCAGAAG 
GCGATGATCA ATGCTATGGA TTCCAAGATC AGATCCCTGG AACAAAGGAT AGTGGAATTG 
TCGGAAGCCA ATAAACTTGC GGCCAACAGC AGTCTCTTTA CCCAGAGGAA CATGAAGGCC 
CAGGAAGAGA TGATTTCAGA ACTCAGGCAA CAGAAGTTTT ACCTGGAGAC GCAAGCTGGG 
AAATTGGAGG CCCAGAACCG GAAGCTGGAG GAGCAGCTGG AAAAAATAAG CCACCAAGAC 
CACAGTGACA AGAACCGTCT GCTGGAGCTG GAGACCAGGC TAAGGGAGGT CAGTCTAGAA 
CATGAAGAGC AGAAACTGGA GCTCAAACGC CAGCTCACAG AGCTGCAGCT CTCCCTGCAG 
GAGCGTGAAT CCCAGCTGAC AGCCCTGCAG GCAGCACGGG CGGCCCTGGA GAGCCAGCTC 
CGCCAGGCCA AGACGGAGCT GGAAGAGACG ACAGCAGAAG CAGAAGAAGA GATCCAGGCG 
CTTACGGCAC ATAGAGATGA AATCCAGCGC AAATTCGATG CCCTTCGTAA CAGCTGTACT 
GTGATCACGG ACTTGGAGGA GCAGCTAAAC CAGCTGACGG AGGACAATGC CGAGCTCAAC 
AACCAAAACT TCTACTTGTC TAAACAACTC GATGAGGCCT CTGGTGCCAA CGACGAGATT 
GTGCAGCTGC GAAGTGAAGT GGACCATCTC CGCCGCGAGA TCACTGAGAG GGAGATGCAG 
CTCACTAGCC AGAAGCAAAC GATGGAGGCG CTGAAGACCA CGTGCACAAT GCTGGAGGAG 
CAGGTCATGG ACCTGGAGGC CCTGAACGAC GAGCTGCTGG AGAAGGAGCG GCAGTGGGAG 
GCCTGGAGGA GTGTCCTTGG TGACGAGAAG TCCCAGTTTG AGTGTCGGGT TCGAGAGTTG 
CAGAGGATGC TGGACACCGA AAAGCAGAGC AGGGCGAGGG CCGACCAGAG GATCACTGAG 
TCGCGCCAGG TGGTCGAGCT GGCAGTAAAG GAGCACAAGG CCGAGATCCT GGCCTTGCAG 
CAGGCTCTCA AGGAGCAGAA GCTGAAGGCC GAGAGCCTCT CCGACAAGCT CAATGATCTG 
GAGAAGAAGC ACGCCATGCT TGAAATGAAT GCCCGAAGTT TACAACAGAA GCTGGAGACA 
GAACGAGAGC TCAAACAAAG GCTTCTGGAA GAGCAAGCCA AGTTACAGCA GCAGATGGAC 
CTGCAGAAGA ATCATATTTT CCGTCTGACT CAAGGGCTGC AAGAAGCTCT GGATCGGGCT 
GATCTGCTGA AGACAGAAAG GAGTGACCTG GAATATCAGC TGGAAAACAT TCAGGTTCTC 
TATTCTCATG AGAAGGTGAA GATGGAAGGC ACTATTTCCC AGCAAACCAA ACTCATCGAT 
TTTCTGCAAG CCAAAATGGA CCAGCCTGCC AAAAAGAAAA AGGGTTTATT TAGTCGACGG 
AAAGAGGACC CTGCTTTGCC CACACAGGTT CCTCTGCAGT ACAATGAGCT GAAGGTGGCT 
CTGGAGAAGG AGAAAGCTCG CTGCGCGGAG CTCGAGGAAG CCCTGCAGAA GACCCGCATC 
GAGCTCCGCT CCGCCCGGGA GGAAGCTGCC CACCGGAAAG CTGCAGACCA CCCACACCCA 
TCTACGCCGG CTACCGCCAG GCAGCAGATC GCCATGTCCG CCATTGTGCG GTCACCCGAG 
CACCAGCCCA GCGCCCTGAG CCTGCTCGCC CCGCCGTCCA GCCGCAGGAA GGAGTCTTCC 
ACTCCAGAGG AATTCAGCCG GCGTCTTAAG GAGCGCATGC ACCACAACAT CCCTCACCGG 
TTCAACGTGG GACTGAACAT GCGAGCCACG AAGTGCGCCG TGTGTCTGGA TACCGTGCAC 
TTCGGGCGCC AGGCATCCAA GTGTCTCGAA TGTCAGGTCA TGTGCCACCC CAAGTGCTCC 
ACATGCTTGC CAGCCACCTG TGGCCTGCCA GCCGAATACG CCACACACTT CACCGAGGCC 
TTCTGCCGCG ACAAAATGAA CTCCCCGGGT CTGCAGTCCA AGGAGCCCAG CAGCAGCCTG 
CACCTGGAAG GGTGGATGAA GGTGCCCAGG AATAACAAGC GAGGACAGCA AGGCTGGGAC 
AGGAAGTACA TCGTTCTGGA GGGCTCCAAA GTCCTCATTT ATGACAACGA AGCCAGAGAA 
GCTGGACAGA GGCCGGTGGA AGAATTTGAG CTGTGCCTTC CCGACGGGGA CGTGTCTATT 
CATGGCGCCG TTGGTGCTTC TGAACTTGCA AACACAGCCA AAGCAGATGT CCCCTATGTC 
CTGAAGATGG AGTCTCACCC CCATACCACC TGCTGGCCTG GGAGGACCCT CTACTTGCTG 
GCTCCCAGCT TTCCCGACAA GCAGCGCTGG GTCACCGCCT TAGAGTCAGT TGTGGCAGGT 
GGGAGAGTGT CTAGGGAAAA GGCGGAAGCC GACGCTGGCC GTGACTGTAC TTCCTACGAG 
CCTCTGCCCG CGTGGGCTCA GAAATTGCTC GGAAACTCCC TGTTGAAACT GGAAGGTGAT 
GACCGTCTGG ACATGAACTG CACGCTGCCC TTCAGCGACC AGGTGGTGCT GGTGGGCACC 
GAGGAAGGGC TGTATGCGCT GAATGTCTTG AAGAACTCCC TCACCCACGT CCCAGGAATC 
GGAGCAGTCT TCCAGATTTA CATCATCAAG GACCTGGAGA AGCTCCTCAT GATCGCAGGA 
GAGGAGCGGG CCCTGTGTCT CGTGGATGTG AAGAAGGTGA AGCAGTCCCT CGCGCAGTCT 
CACCTCCCCG CCCAGCCAGA CATCTCGCCC AACATCTTTG AAGCGGTCAA GGGCTGCCAC 
TTGTTTGCTG CTGGCAAGAT TGAGAGCGGG CTCTGCATCT GTGCAGCCAT GCCCAGCAAA 
GTTGTCATTC TCCGCTACAA TGAAAACCTC AGCAAGTACT GCATTCGCAA AGAGATCGAG 
ACCTCGGAGC CCTGCAGCTG TATCCACTTC ACCAACTACA GTATCCTCAT CGGAACCAAT 
AAATTCTACG AAATCGACAT GAAGCAGTAC ACGCTAGAGG AATTCCTGGA TAAGAACGAC 
CATTCCTTGG CGCCTGCCGT GTTTGCCTCC TCTTCCAACA GCTTCCCCGT GTCCATCATG 
CAGGTGAACG GCGCGGGGCA GCGGGAGGAG TTCCTGCTCT GTTTCCACGA ATTCGGGGTG 
TTCGTGGATT CTTATGGAAG ACGCAGCCGC ACAGATGATC TCAAGTGGAG TCGCTTACCT 
CTGGCCTTTG CCTACAGAGA ACCCTATCTG TTTGTGACTC ACTTCAACTC ACTCGAAGTA 
ATTGAGATCC AGGCCCGCTC CTCTCTGGGG ACCCCCGCCC GAGCATATTT GGAAATCCCC 
AACCCACGCT ACCTGGGCCC TGCAATCTCC TCAGGAGCCA TCTACCTGGC GTCCTCATAT 
CAGGATAAGT TAAGGGTCAT CTGCTGCAAA GGAAACCTGG TGAAGGAGAC CGGCACCGAC 
CACCACCGTG GCCCATCCAC CTCCCGCAGC CCCAACAAGC GAGGCCCGCC GACGTACAAC 
GAGCACATCA CCAAGCGCGT GGCCTCAAGC CCGGCGCCTC CCGAAGGCCC CAGCCACCCT 
CGAGAGCCAA GCACACCCCA CCGCTACCGC GAGGGGCGGA CCGAGCTGCG CAGGGACAAG 
TCTCCGGGCC GCCCCCTGGA GCGCGAGAAG TCCCCCGGCC GGATGCTGAG CACGCGGAGG 
GAGCGCTCCC CCGGGCGGCT GTTTGAGGAC AGCAGCAGGG GCCGGCTGCC CGTGGGGGCC 
GTGAGGACCC CGCTGTCCCA GGTCAACAAG GTCTGGGACC AGTCTTCAGT ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024833512.1
CDS	330..5183
Translation	MMEQEMTRLHRRVSEVEAVLSQKEVELKASETQRSLLEQDLATYITECSSLKRSLEQARMEVSQEDDKALQLLHDIREQSRKLQEIKEQEYQAQVEEMRLMMNQLEEDLVSARRRSDLYESELRESRLAAEEFKRKATECQHKLMKAKDQGKPEVGEYAKLEKINAEQQLKIQELQEKLEKAVKASTEATELLQNIRQAKERAERELEKLHNREDSSEGIKKKLVEAEERRHSLENKVKRLETMERRENRLKDDIQTKSQQIQQMADKILELEEKHREAQVSAQHLEVHLKQKEQHYEEKIKVLDNQIKKDLADKETLENLMQRHEEEAHEKGKILSEQKAMINAMDSKIRSLEQRIVELSEANKLAANSSLFTQRNMKAQEEMISELRQQKFYLETQAGKLEAQNRKLEEQLEKISHQDHSDKNRLLELETRLREVSLEHEEQKLELKRQLTELQLSLQERESQLTALQAARAALESQLRQAKTELEETTAEAEEEIQALTAHRDEIQRKFDALRNSCTVITDLEEQLNQLTEDNAELNNQNFYLSKQLDEASGANDEIVQLRSEVDHLRREITEREMQLTSQKQTMEALKTTCTMLEEQVMDLEALNDELLEKERQWEAWRSVLGDEKSQFECRVRELQRMLDTEKQSRARADQRITESRQVVELAVKEHKAEILALQQALKEQKLKAESLSDKLNDLEKKHAMLEMNARSLQQKLETERELKQRLLEEQAKLQQQMDLQKNHIFRLTQGLQEALDRADLLKTERSDLEYQLENIQVLYSHEKVKMEGTISQQTKLIDFLQAKMDQPAKKKKGLFSRRKEDPALPTQVPLQYNELKVALEKEKARCAELEEALQKTRIELRSAREEAAHRKAADHPHPSTPATARQQIAMSAIVRSPEHQPSALSLLAPPSSRRKESSTPEEFSRRLKERMHHNIPHRFNVGLNMRATKCAVCLDTVHFGRQASKCLECQVMCHPKCSTCLPATCGLPAEYATHFTEAFCRDKMNSPGLQSKEPSSSLHLEGWMKVPRNNKRGQQGWDRKYIVLEGSKVLIYDNEAREAGQRPVEEFELCLPDGDVSIHGAVGASELANTAKADVPYVLKMESHPHTTCWPGRTLYLLAPSFPDKQRWVTALESVVAGGRVSREKAEADAGRDCTSYEPLPAWAQKLLGNSLLKLEGDDRLDMNCTLPFSDQVVLVGTEEGLYALNVLKNSLTHVPGIGAVFQIYIIKDLEKLLMIAGEERALCLVDVKKVKQSLAQSHLPAQPDISPNIFEAVKGCHLFAAGKIESGLCICAAMPSKVVILRYNENLSKYCIRKEIETSEPCSCIHFTNYSILIGTNKFYEIDMKQYTLEEFLDKNDHSLAPAVFASSSNSFPVSIMQVNGAGQREEFLLCFHEFGVFVDSYGRRSRTDDLKWSRLPLAFAYREPYLFVTHFNSLEVIEIQARSSLGTPARAYLEIPNPRYLGPAISSGAIYLASSYQDKLRVICCKGNLVKETGTDHHRGPSTSRSPNKRGPPTYNEHITKRVASSPAPPEGPSHPREPSTPHRYREGRTELRRDKSPGRPLEREKSPGRMLSTRRERSPGRLFEDSSRGRLPVGAVRTPLSQVNKVWDQSSV

Target ORF information:

RefSeq Version	XM_024977744.1
Organism	Bos taurus(cattle)
Definition	Bos taurus citron rho-interacting serine/threonine kinase (CIT), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024977744.1

ORF Insert Sequence:

ATGATGGAGC AGGAAATGAC CCGGTTACAT CGGAGAGTGT CAGAGGTGGA GGCTGTGCTT 
AGTCAGAAGG AGGTGGAGCT GAAGGCCTCT GAGACTCAGA GATCCCTCCT GGAGCAGGAC 
CTTGCTACCT ACATCACAGA ATGCAGTAGC TTAAAGCGAA GTTTGGAGCA AGCACGGATG 
GAGGTGTCCC AAGAGGACGA CAAAGCACTG CAGCTCCTCC ATGATATCAG AGAGCAGAGC 
CGGAAGCTCC AAGAAATCAA AGAGCAGGAG TACCAGGCTC AAGTGGAAGA AATGAGGTTG 
ATGATGAATC AGTTGGAAGA GGACCTGGTT TCGGCGAGAA GGCGGAGTGA CCTCTATGAA 
TCGGAGCTCA GAGAGTCTCG GCTGGCTGCT GAAGAGTTTA AGCGGAAAGC AACAGAATGT 
CAGCATAAAC TGATGAAGGC TAAAGATCAA GGGAAGCCTG AAGTGGGAGA ATATGCCAAA 
CTGGAGAAGA TCAATGCTGA GCAGCAGCTC AAAATTCAGG AGCTCCAAGA GAAGCTAGAA 
AAGGCTGTGA AAGCTAGCAC AGAGGCCACC GAGCTGCTGC AGAACATCCG TCAGGCGAAG 
GAGCGGGCCG AGCGTGAGCT GGAGAAGCTG CACAACCGTG AGGACTCCTC CGAGGGCATC 
AAGAAGAAGC TGGTGGAGGC CGAGGAACGC CGCCACTCTC TGGAGAACAA GGTAAAGAGG 
CTAGAGACCA TGGAGCGTAG AGAAAACAGA CTGAAGGATG ACATCCAGAC AAAGTCCCAA 
CAGATCCAGC AGATGGCTGA TAAAATTCTG GAGCTGGAGG AGAAGCACAG AGAAGCCCAG 
GTCTCAGCGC AGCATCTGGA GGTGCACCTG AAACAGAAAG AGCAGCACTA TGAAGAAAAA 
ATTAAAGTGT TGGACAATCA GATAAAGAAA GACCTAGCAG ACAAGGAGAC ACTGGAGAAC 
CTGATGCAAA GACACGAAGA GGAGGCCCAT GAGAAAGGCA AAATTCTCAG CGAGCAGAAG 
GCGATGATCA ATGCTATGGA TTCCAAGATC AGATCCCTGG AACAAAGGAT AGTGGAATTG 
TCGGAAGCCA ATAAACTTGC GGCCAACAGC AGTCTCTTTA CCCAGAGGAA CATGAAGGCC 
CAGGAAGAGA TGATTTCAGA ACTCAGGCAA CAGAAGTTTT ACCTGGAGAC GCAAGCTGGG 
AAATTGGAGG CCCAGAACCG GAAGCTGGAG GAGCAGCTGG AAAAAATAAG CCACCAAGAC 
CACAGTGACA AGAACCGTCT GCTGGAGCTG GAGACCAGGC TAAGGGAGGT CAGTCTAGAA 
CATGAAGAGC AGAAACTGGA GCTCAAACGC CAGCTCACAG AGCTGCAGCT CTCCCTGCAG 
GAGCGTGAAT CCCAGCTGAC AGCCCTGCAG GCAGCACGGG CGGCCCTGGA GAGCCAGCTC 
CGCCAGGCCA AGACGGAGCT GGAAGAGACG ACAGCAGAAG CAGAAGAAGA GATCCAGGCG 
CTTACGGCAC ATAGAGATGA AATCCAGCGC AAATTCGATG CCCTTCGTAA CAGCTGTACT 
GTGATCACGG ACTTGGAGGA GCAGCTAAAC CAGCTGACGG AGGACAATGC CGAGCTCAAC 
AACCAAAACT TCTACTTGTC TAAACAACTC GATGAGGCCT CTGGTGCCAA CGACGAGATT 
GTGCAGCTGC GAAGTGAAGT GGACCATCTC CGCCGCGAGA TCACTGAGAG GGAGATGCAG 
CTCACTAGCC AGAAGCAAAC GATGGAGGCG CTGAAGACCA CGTGCACAAT GCTGGAGGAG 
CAGGTCATGG ACCTGGAGGC CCTGAACGAC GAGCTGCTGG AGAAGGAGCG GCAGTGGGAG 
GCCTGGAGGA GTGTCCTTGG TGACGAGAAG TCCCAGTTTG AGTGTCGGGT TCGAGAGTTG 
CAGAGGATGC TGGACACCGA AAAGCAGAGC AGGGCGAGGG CCGACCAGAG GATCACTGAG 
TCGCGCCAGG TGGTCGAGCT GGCAGTAAAG GAGCACAAGG CCGAGATCCT GGCCTTGCAG 
CAGGCTCTCA AGGAGCAGAA GCTGAAGGCC GAGAGCCTCT CCGACAAGCT CAATGATCTG 
GAGAAGAAGC ACGCCATGCT TGAAATGAAT GCCCGAAGTT TACAACAGAA GCTGGAGACA 
GAACGAGAGC TCAAACAAAG GCTTCTGGAA GAGCAAGCCA AGTTACAGCA GCAGATGGAC 
CTGCAGAAGA ATCATATTTT CCGTCTGACT CAAGGGCTGC AAGAAGCTCT GGATCGGGCT 
GATCTGCTGA AGACAGAAAG GAGTGACCTG GAATATCAGC TGGAAAACAT TCAGGTTCTC 
TATTCTCATG AGAAGGTGAA GATGGAAGGC ACTATTTCCC AGCAAACCAA ACTCATCGAT 
TTTCTGCAAG CCAAAATGGA CCAGCCTGCC AAAAAGAAAA AGGGTTTATT TAGTCGACGG 
AAAGAGGACC CTGCTTTGCC CACACAGGTT CCTCTGCAGT ACAATGAGCT GAAGGTGGCT 
CTGGAGAAGG AGAAAGCTCG CTGCGCGGAG CTCGAGGAAG CCCTGCAGAA GACCCGCATC 
GAGCTCCGCT CCGCCCGGGA GGAAGCTGCC CACCGGAAAG CTGCAGACCA CCCACACCCA 
TCTACGCCGG CTACCGCCAG GCAGCAGATC GCCATGTCCG CCATTGTGCG GTCACCCGAG 
CACCAGCCCA GCGCCCTGAG CCTGCTCGCC CCGCCGTCCA GCCGCAGGAA GGAGTCTTCC 
ACTCCAGAGG AATTCAGCCG GCGTCTTAAG GAGCGCATGC ACCACAACAT CCCTCACCGG 
TTCAACGTGG GACTGAACAT GCGAGCCACG AAGTGCGCCG TGTGTCTGGA TACCGTGCAC 
TTCGGGCGCC AGGCATCCAA GTGTCTCGAA TGTCAGGTCA TGTGCCACCC CAAGTGCTCC 
ACATGCTTGC CAGCCACCTG TGGCCTGCCA GCCGAATACG CCACACACTT CACCGAGGCC 
TTCTGCCGCG ACAAAATGAA CTCCCCGGGT CTGCAGTCCA AGGAGCCCAG CAGCAGCCTG 
CACCTGGAAG GGTGGATGAA GGTGCCCAGG AATAACAAGC GAGGACAGCA AGGCTGGGAC 
AGGAAGTACA TCGTTCTGGA GGGCTCCAAA GTCCTCATTT ATGACAACGA AGCCAGAGAA 
GCTGGACAGA GGCCGGTGGA AGAATTTGAG CTGTGCCTTC CCGACGGGGA CGTGTCTATT 
CATGGCGCCG TTGGTGCTTC TGAACTTGCA AACACAGCCA AAGCAGATGT CCCCTATGTC 
CTGAAGATGG AGTCTCACCC CCATACCACC TGCTGGCCTG GGAGGACCCT CTACTTGCTG 
GCTCCCAGCT TTCCCGACAA GCAGCGCTGG GTCACCGCCT TAGAGTCAGT TGTGGCAGGT 
GGGAGAGTGT CTAGGGAAAA GGCGGAAGCC GACGCTGGCC GTGACTGTAC TTCCTACGAG 
CCTCTGCCCG CGTGGGCTCA GAAATTGCTC GGAAACTCCC TGTTGAAACT GGAAGGTGAT 
GACCGTCTGG ACATGAACTG CACGCTGCCC TTCAGCGACC AGGTGGTGCT GGTGGGCACC 
GAGGAAGGGC TGTATGCGCT GAATGTCTTG AAGAACTCCC TCACCCACGT CCCAGGAATC 
GGAGCAGTCT TCCAGATTTA CATCATCAAG GACCTGGAGA AGCTCCTCAT GATCGCAGGA 
GAGGAGCGGG CCCTGTGTCT CGTGGATGTG AAGAAGGTGA AGCAGTCCCT CGCGCAGTCT 
CACCTCCCCG CCCAGCCAGA CATCTCGCCC AACATCTTTG AAGCGGTCAA GGGCTGCCAC 
TTGTTTGCTG CTGGCAAGAT TGAGAGCGGG CTCTGCATCT GTGCAGCCAT GCCCAGCAAA 
GTTGTCATTC TCCGCTACAA TGAAAACCTC AGCAAGTACT GCATTCGCAA AGAGATCGAG 
ACCTCGGAGC CCTGCAGCTG TATCCACTTC ACCAACTACA GTATCCTCAT CGGAACCAAT 
AAATTCTACG AAATCGACAT GAAGCAGTAC ACGCTAGAGG AATTCCTGGA TAAGAACGAC 
CATTCCTTGG CGCCTGCCGT GTTTGCCTCC TCTTCCAACA GCTTCCCCGT GTCCATCATG 
CAGGTGAACG GCGCGGGGCA GCGGGAGGAG TTCCTGCTCT GTTTCCACGA ATTCGGGGTG 
TTCGTGGATT CTTATGGAAG ACGCAGCCGC ACAGATGATC TCAAGTGGAG TCGCTTACCT 
CTGGCCTTTG CCTACAGAGA ACCCTATCTG TTTGTGACTC ACTTCAACTC ACTCGAAGTA 
ATTGAGATCC AGGCCCGCTC CTCTCTGGGG ACCCCCGCCC GAGCATATTT GGAAATCCCC 
AACCCACGCT ACCTGGGCCC TGCAATCTCC TCAGGAGCCA TCTACCTGGC GTCCTCATAT 
CAGGATAAGT TAAGGGTCAT CTGCTGCAAA GGAAACCTGG TGAAGGAGAC CGGCACCGAC 
CACCACCGTG GCCCATCCAC CTCCCGCAGC CCCAACAAGC GAGGCCCGCC GACGTACAAC 
GAGCACATCA CCAAGCGCGT GGCCTCAAGC CCGGCGCCTC CCGAAGGCCC CAGCCACCCT 
CGAGAGCCAA GCACACCCCA CCGCTACCGC GAGGGGCGGA CCGAGCTGCG CAGGGACAAG 
TCTCCGGGCC GCCCCCTGGA GCGCGAGAAG TCCCCCGGCC GGATGCTGAG CACGCGGAGG 
GAGCGCTCCC CCGGGCGGCT GTTTGAGGAC AGCAGCAGGG GCCGGCTGCC CGTGGGGGCC 
GTGAGGACCC CGCTGTCCCA GGTCAACAAG GTCTGGGACC AGTCTTCAGT ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CIT ( XM_015475534.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015331020.1 Bos taurus citron rho-interacting serine/threonine kinase (CIT), mRNA.

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CloneID	OBa93236
Clone ID Related Accession (Same CDS sequence)	XM_015475534.1 , XM_015467110.1
Accession Version	XM_015475534.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5979bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	citron Rho-interacting kinase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104457.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

ATGTTGAAGT TCAAGTATGG AGCACGGAAT CTACTGGACG CTGGTACTGT TGAATCCATT 
GCCAGCCGGG CCTCCAGGCT GAATCTTTTC TTCCAGGGGA AACCACCCTC CATGACTCAA 
CAGCAGATGT CTCTTCTTTC CCGAGAAGCG ATATTAGATG CCCTCTTTGT TCTCTTTAAA 
GAATGCAGTC AGCCAGCTCT GATGAAGATT AAGCACGTGA GCAACTTTGT CCAAAAGTGT 
AAGTGTGGGG AGAACTTTTT CTTGAAAATT GTCCTGCTTA GTGAGAATGT CATGGAATAT 
CAGCCTGGAG GGGATTTGCT GTCACTTTTG AATAGATATG AGGACCAATT AGATGAAAAT 
ATGATTCAGT TTTACCTAGC TGAACTGATT TTGGCTGTCC ACAGCGTTCA TCAGATGGGC 
TATGTACATC GAGACATCAA GCCCGAGAAC ATCCTCATTG ACCGAACGGG ACACATCAAG 
CTGGTGGATT TTGGATCAGC TGCTAAAATG AACTCAAATA AGATGGTAAA TGCCAAACTC 
CCGGTTGGTA CTCCAGATTA CATGGCCCCT GAAGTGTTGA CTGTGATGAA TGGGGATGGA 
AAAGGTGCCT ATAGCCTAGA CTGTGATTGG TGGTCAGTGG GAGTTATTGC TTATGAGATG 
GTTTATGGAA GATGCCCATT CACTGAAGGA ACCTCAGCCA AAACCTTCAA TAACATCATG 
AATTTCCAGC GGTTCTTGAA GTTTCCAGAT GACCCCAAAG TTAGCAGTGA ATTGCTTGAT 
CTAATCCAGA GTTTGTTGTG TGGCCAGAAA GAGAGACTGA AGTTTGAGGG CCTTTGCTGT 
CATCCTTTCT TCTCCAAAAT TGACTGGATT AACATTCGTA ACTCTCCTCC CCCCTTCGTT 
CCCACCCTCA AGTCTGATGA TGACACCTCC AATTTTGATG AACCAGAGAA GAATTCGTGG 
GTTTCATCCT CTCCGTGCCA GCTGAACCCC TCAGGTTTCT CGGGCGAAGA ACTGCCGTTT 
GTGGGGTTTT CGTATAGCAA GGCACTGGGA ATTCTTGGTA GATCTGAGCC TGTTGTATCA 
AGTCTGGACT CCCCTGCCAA GACTAGCTCC ATGGAAAAGA AACTCCTTAT CAAAAGCAAA 
GAGCTGCAGG ACTCCCAGGA CAAGTGTCAC AAGATGGAGC AGGAAATGAC CCGGTTACAT 
CGGAGAGTGT CAGAGGTGGA GGCTGTGCTT AGTCAGAAGG AGGTGGAGCT GAAGGCCTCT 
GAGACTCAGA GATCCCTCCT GGAGCAGGAC CTTGCTACCT ACATCACAGA ATGCAGTAGC 
TTAAAGCGAA GTTTGGAGCA AGCACGGATG GAGGTGTCCC AAGAGGACGA CAAAGCACTG 
CAGCTCCTCC ATGATATCAG AGAGCAGAGC CGGAAGCTCC AAGAAATCAA AGAGCAGGAG 
TACCAGGCTC AAGTGGAAGA AATGAGGTTG ATGATGAATC AGTTGGAAGA GGACCTGGTT 
TCGGCGAGAA GGCGGAGTGA CCTCTATGAA TCGGAGCTCA GAGAGTCTCG GCTGGCTGCT 
GAAGAGTTTA AGCGGAAAGC AACAGAATGT CAGCATAAAC TGATGAAGGC TAAAGATCAA 
GGGAAGCCTG AAGTGGGAGA ATATGCCAAA CTGGAGAAGA TCAATGCTGA GCAGCAGCTC 
AAAATTCAGG AGCTCCAAGA GAAGCTAGAA AAGGCTGTGA AAGCTAGCAC AGAGGCCACC 
GAGCTGCTGC AGAACATCCG TCAGGCGAAG GAGCGGGCCG AGCGTGAGCT GGAGAAGCTG 
CACAACCGTG AGGACTCCTC CGAGGGCATC AAGAAGAAGC TGGTGGAGGC CGAGGAACGC 
CGCCACTCTC TGGAGAACAA GGTAAAGAGG CTAGAGACCA TGGAGCGTAG AGAAAACAGA 
CTGAAGGATG ACATCCAGAC AAAGTCCCAA CAGATCCAGC AGATGGCTGA TAAAATTCTG 
GAGCTGGAGG AGAAGCACAG AGAAGCCCAG GTCTCAGCGC AGCATCTGGA GGTGCACCTG 
AAACAGAAAG AGCAGCACTA TGAAGAAAAA ATTAAAGTGT TGGACAATCA GATAAAGAAA 
GACCTAGCAG ACAAGGAGAC ACTGGAGAAC CTGATGCAAA GACACGAAGA GGAGGCCCAT 
GAGAAAGGCA AAATTCTCAG CGAGCAGAAG GCGATGATCA ATGCTATGGA TTCCAAGATC 
AGATCCCTGG AACAAAGGAT AGTGGAATTG TCGGAAGCCA ATAAACTTGC GGCCAACAGC 
AGTCTCTTTA CCCAGAGGAA CATGAAGGCC CAGGAAGAGA TGATTTCAGA ACTCAGGCAA 
CAGAAGTTTT ACCTGGAGAC GCAAGCTGGG AAATTGGAGG CCCAGAACCG GAAGCTGGAG 
GAGCAGCTGG AAAAAATAAG CCACCAAGAC CACAGTGACA AGAACCGTCT GCTGGAGCTG 
GAGACCAGGC TAAGGGAGGT CAGTCTAGAA CATGAAGAGC AGAAACTGGA GCTCAAACGC 
CAGCTCACAG AGCTGCAGCT CTCCCTGCAG GAGCGTGAAT CCCAGCTGAC AGCCCTGCAG 
GCAGCACGGG CGGCCCTGGA GAGCCAGCTC CGCCAGGCCA AGACGGAGCT GGAAGAGACG 
ACAGCAGAAG CAGAAGAAGA GATCCAGGCG CTTACGGCAC ATAGAGATGA AATCCAGCGC 
AAATTCGATG CCCTTCGTAA CAGCTGTACT GTGATCACGG ACTTGGAGGA GCAGCTAAAC 
CAGCTGACGG AGGACAATGC CGAGCTCAAC AACCAAAACT TCTACTTGTC TAAACAACTC 
GATGAGGCCT CTGGTGCCAA CGACGAGATT GTGCAGCTGC GAAGTGAAGT GGACCATCTC 
CGCCGCGAGA TCACTGAGAG GGAGATGCAG CTCACTAGCC AGAAGCAAAC GATGGAGGCG 
CTGAAGACCA CGTGCACAAT GCTGGAGGAG CAGGTCATGG ACCTGGAGGC CCTGAACGAC 
GAGCTGCTGG AGAAGGAGCG GCAGTGGGAG GCCTGGAGGA GTGTCCTTGG TGACGAGAAG 
TCCCAGTTTG AGTGTCGGGT TCGAGAGTTG CAGAGGATGC TGGACACCGA AAAGCAGAGC 
AGGGCGAGGG CCGACCAGAG GATCACTGAG TCGCGCCAGG TGGTCGAGCT GGCAGTAAAG 
GAGCACAAGG CCGAGATCCT GGCCTTGCAG CAGGCTCTCA AGGAGCAGAA GCTGAAGGCC 
GAGAGCCTCT CCGACAAGCT CAATGATCTG GAGAAGAAGC ACGCCATGCT TGAAATGAAT 
GCCCGAAGTT TACAACAGAA GCTGGAGACA GAACGAGAGC TCAAACAAAG GCTTCTGGAA 
GAGCAAGCCA AGTTACAGCA GCAGATGGAC CTGCAGAAGA ATCATATTTT CCGTCTGACT 
CAAGGGCTGC AAGAAGCTCT GGATCGGGCT GATCTGCTGA AGACAGAAAG GAGTGACCTG 
GAATATCAGC TGGAAAACAT TCAGGTTCTC TATTCTCATG AGAAGGTGAA GATGGAAGGC 
ACTATTTCCC AGCAAACCAA ACTCATCGAT TTTCTGCAAG CCAAAATGGA CCAGCCTGCC 
AAAAAGAAAA AGGGTTTATT TAGTCGACGG AAAGAGGACC CTGCTTTGCC CACACAGGTT 
CCTCTGCAGT ACAATGAGCT GAAGGTGGCT CTGGAGAAGG AGAAAGCTCG CTGCGCGGAG 
CTCGAGGAAG CCCTGCAGAA GACCCGCATC GAGCTCCGCT CCGCCCGGGA GGAAGCTGCC 
CACCGGAAAG CTGCAGACCA CCCACACCCA TCTACGCCGG CTACCGCCAG GCAGCAGATC 
GCCATGTCCG CCATTGTGCG GTCACCCGAG CACCAGCCCA GCGCCCTGAG CCTGCTCGCC 
CCGCCGTCCA GCCGCAGGAA GGAGTCTTCC ACTCCAGAGG AATTCAGCCG GCGTCTTAAG 
GAGCGCATGC ACCACAACAT CCCTCACCGG TTCAACGTGG GACTGAACAT GCGAGCCACG 
AAGTGCGCCG TGTGTCTGGA TACCGTGCAC TTCGGGCGCC AGGCATCCAA GTGTCTCGAA 
TGTCAGGTCA TGTGCCACCC CAAGTGCTCC ACATGCTTGC CAGCCACCTG TGGCCTGCCA 
GCCGAATACG CCACACACTT CACCGAGGCC TTCTGCCGCG ACAAAATGAA CTCCCCGGGT 
CTGCAGTCCA AGGAGCCCAG CAGCAGCCTG CACCTGGAAG GGTGGATGAA GGTGCCCAGG 
AATAACAAGC GAGGACAGCA AGGCTGGGAC AGGAAGTACA TCGTTCTGGA GGGCTCCAAA 
GTCCTCATTT ATGACAACGA AGCCAGAGAA GCTGGACAGA GGCCGGTGGA AGAATTTGAG 
CTGTGCCTTC CCGACGGGGA CGTGTCTATT CATGGCGCCG TTGGTGCTTC TGAACTTGCA 
AACACAGCCA AAGCAGATGT CCCCTATGTC CTGAAGATGG AGTCTCACCC CCATACCACC 
TGCTGGCCTG GGAGGACCCT CTACTTGCTG GCTCCCAGCT TTCCCGACAA GCAGCGCTGG 
GTCACCGCCT TAGAGTCAGT TGTGGCAGGT GGGAGAGTGT CTAGGGAAAA GGCGGAAGCC 
GACGCTAAAT TGCTCGGAAA CTCCCTGTTG AAACTGGAAG GTGATGACCG TCTGGACATG 
AACTGCACGC TGCCCTTCAG CGACCAGGTG GTGCTGGTGG GCACCGAGGA AGGGCTGTAT 
GCGCTGAATG TCTTGAAGAA CTCCCTCACC CACGTCCCAG GAATCGGAGC AGTCTTCCAG 
ATTTACATCA TCAAGGACCT GGAGAAGCTC CTCATGATCG CAGGAGAGGA GCGGGCCCTG 
TGTCTCGTGG ATGTGAAGAA GGTGAAGCAG TCCCTCGCGC AGTCTCACCT CCCCGCCCAG 
CCAGACATCT CGCCCAACAT CTTTGAAGCG GTCAAGGGCT GCCACTTGTT TGCTGCTGGC 
AAGATTGAGA GCGGGCTCTG CATCTGTGCA GCCATGCCCA GCAAAGTTGT CATTCTCCGC 
TACAATGAAA ACCTCAGCAA GTACTGCATT CGCAAAGAGA TCGAGACCTC GGAGCCCTGC 
AGCTGTATCC ACTTCACCAA CTACAGTATC CTCATCGGAA CCAATAAATT CTACGAAATC 
GACATGAAGC AGTACACGCT AGAGGAATTC CTGGATAAGA ACGACCATTC CTTGGCGCCT 
GCCGTGTTTG CCTCCTCTTC CAACAGCTTC CCCGTGTCCA TCATGCAGGT GAACGGCGCG 
GGGCAGCGGG AGGAGTTCCT GCTCTGTTTC CACGAATTCG GGGTGTTCGT GGATTCTTAT 
GGAAGACGCA GCCGCACAGA TGATCTCAAG TGGAGTCGCT TACCTCTGGC CTTTGCCTAC 
AGAGAACCCT ATCTGTTTGT GACTCACTTC AACTCACTCG AAGTAATTGA GATCCAGGCC 
CGCTCCTCTC TGGGGACCCC CGCCCGAGCA TATTTGGAAA TCCCCAACCC ACGCTACCTG 
GGCCCTGCAA TCTCCTCAGG AGCCATCTAC CTGGCGTCCT CATATCAGGA TAAGTTAAGG 
GTCATCTGCT GCAAAGGAAA CCTGGTGAAG GAGACCGGCA CCGACCACCA CCGTGGCCCA 
TCCACCTCCC GCAGCCCCAA CAAGCGAGGC CCGCCGACGT ACAACGAGCA CATCACCAAG 
CGCGTGGCCT CAAGCCCGGC GCCTCCCGAA GGCCCCAGCC ACCCTCGAGA GCCAAGCACA 
CCCCACCGCT ACCGCGAGGG GCGGACCGAG CTGCGCAGGG ACAAGTCTCC GGGCCGCCCC 
CTGGAGCGCG AGAAGTCCCC CGGCCGGATG CTGAGCACGC GGAGGGAGCG CTCCCCCGGG 
CGGCTGTTTG AGGACAGCAG CAGGGGCCGG CTGCCCGTGG GGGCCGTGAG GACCCCGCTG 
TCCCAGGTCA ACAAGGTCTG GGACCAGTCT TCAGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015331020.1
CDS	263..6241
Translation	MLKFKYGARNLLDAGTVESIASRASRLNLFFQGKPPSMTQQQMSLLSREAILDALFVLFKECSQPALMKIKHVSNFVQKCKCGENFFLKIVLLSENVMEYQPGGDLLSLLNRYEDQLDENMIQFYLAELILAVHSVHQMGYVHRDIKPENILIDRTGHIKLVDFGSAAKMNSNKMVNAKLPVGTPDYMAPEVLTVMNGDGKGAYSLDCDWWSVGVIAYEMVYGRCPFTEGTSAKTFNNIMNFQRFLKFPDDPKVSSELLDLIQSLLCGQKERLKFEGLCCHPFFSKIDWINIRNSPPPFVPTLKSDDDTSNFDEPEKNSWVSSSPCQLNPSGFSGEELPFVGFSYSKALGILGRSEPVVSSLDSPAKTSSMEKKLLIKSKELQDSQDKCHKMEQEMTRLHRRVSEVEAVLSQKEVELKASETQRSLLEQDLATYITECSSLKRSLEQARMEVSQEDDKALQLLHDIREQSRKLQEIKEQEYQAQVEEMRLMMNQLEEDLVSARRRSDLYESELRESRLAAEEFKRKATECQHKLMKAKDQGKPEVGEYAKLEKINAEQQLKIQELQEKLEKAVKASTEATELLQNIRQAKERAERELEKLHNREDSSEGIKKKLVEAEERRHSLENKVKRLETMERRENRLKDDIQTKSQQIQQMADKILELEEKHREAQVSAQHLEVHLKQKEQHYEEKIKVLDNQIKKDLADKETLENLMQRHEEEAHEKGKILSEQKAMINAMDSKIRSLEQRIVELSEANKLAANSSLFTQRNMKAQEEMISELRQQKFYLETQAGKLEAQNRKLEEQLEKISHQDHSDKNRLLELETRLREVSLEHEEQKLELKRQLTELQLSLQERESQLTALQAARAALESQLRQAKTELEETTAEAEEEIQALTAHRDEIQRKFDALRNSCTVITDLEEQLNQLTEDNAELNNQNFYLSKQLDEASGANDEIVQLRSEVDHLRREITEREMQLTSQKQTMEALKTTCTMLEEQVMDLEALNDELLEKERQWEAWRSVLGDEKSQFECRVRELQRMLDTEKQSRARADQRITESRQVVELAVKEHKAEILALQQALKEQKLKAESLSDKLNDLEKKHAMLEMNARSLQQKLETERELKQRLLEEQAKLQQQMDLQKNHIFRLTQGLQEALDRADLLKTERSDLEYQLENIQVLYSHEKVKMEGTISQQTKLIDFLQAKMDQPAKKKKGLFSRRKEDPALPTQVPLQYNELKVALEKEKARCAELEEALQKTRIELRSAREEAAHRKAADHPHPSTPATARQQIAMSAIVRSPEHQPSALSLLAPPSSRRKESSTPEEFSRRLKERMHHNIPHRFNVGLNMRATKCAVCLDTVHFGRQASKCLECQVMCHPKCSTCLPATCGLPAEYATHFTEAFCRDKMNSPGLQSKEPSSSLHLEGWMKVPRNNKRGQQGWDRKYIVLEGSKVLIYDNEAREAGQRPVEEFELCLPDGDVSIHGAVGASELANTAKADVPYVLKMESHPHTTCWPGRTLYLLAPSFPDKQRWVTALESVVAGGRVSREKAEADAKLLGNSLLKLEGDDRLDMNCTLPFSDQVVLVGTEEGLYALNVLKNSLTHVPGIGAVFQIYIIKDLEKLLMIAGEERALCLVDVKKVKQSLAQSHLPAQPDISPNIFEAVKGCHLFAAGKIESGLCICAAMPSKVVILRYNENLSKYCIRKEIETSEPCSCIHFTNYSILIGTNKFYEIDMKQYTLEEFLDKNDHSLAPAVFASSSNSFPVSIMQVNGAGQREEFLLCFHEFGVFVDSYGRRSRTDDLKWSRLPLAFAYREPYLFVTHFNSLEVIEIQARSSLGTPARAYLEIPNPRYLGPAISSGAIYLASSYQDKLRVICCKGNLVKETGTDHHRGPSTSRSPNKRGPPTYNEHITKRVASSPAPPEGPSHPREPSTPHRYREGRTELRRDKSPGRPLEREKSPGRMLSTRRERSPGRLFEDSSRGRLPVGAVRTPLSQVNKVWDQSSV

Target ORF information:

RefSeq Version	XM_015475534.1
Organism	Bos taurus(cattle)
Definition	Bos taurus citron rho-interacting serine/threonine kinase (CIT), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015475534.1

ORF Insert Sequence:

ATGTTGAAGT TCAAGTATGG AGCACGGAAT CTACTGGACG CTGGTACTGT TGAATCCATT 
GCCAGCCGGG CCTCCAGGCT GAATCTTTTC TTCCAGGGGA AACCACCCTC CATGACTCAA 
CAGCAGATGT CTCTTCTTTC CCGAGAAGCG ATATTAGATG CCCTCTTTGT TCTCTTTAAA 
GAATGCAGTC AGCCAGCTCT GATGAAGATT AAGCACGTGA GCAACTTTGT CCAAAAGTGT 
AAGTGTGGGG AGAACTTTTT CTTGAAAATT GTCCTGCTTA GTGAGAATGT CATGGAATAT 
CAGCCTGGAG GGGATTTGCT GTCACTTTTG AATAGATATG AGGACCAATT AGATGAAAAT 
ATGATTCAGT TTTACCTAGC TGAACTGATT TTGGCTGTCC ACAGCGTTCA TCAGATGGGC 
TATGTACATC GAGACATCAA GCCCGAGAAC ATCCTCATTG ACCGAACGGG ACACATCAAG 
CTGGTGGATT TTGGATCAGC TGCTAAAATG AACTCAAATA AGATGGTAAA TGCCAAACTC 
CCGGTTGGTA CTCCAGATTA CATGGCCCCT GAAGTGTTGA CTGTGATGAA TGGGGATGGA 
AAAGGTGCCT ATAGCCTAGA CTGTGATTGG TGGTCAGTGG GAGTTATTGC TTATGAGATG 
GTTTATGGAA GATGCCCATT CACTGAAGGA ACCTCAGCCA AAACCTTCAA TAACATCATG 
AATTTCCAGC GGTTCTTGAA GTTTCCAGAT GACCCCAAAG TTAGCAGTGA ATTGCTTGAT 
CTAATCCAGA GTTTGTTGTG TGGCCAGAAA GAGAGACTGA AGTTTGAGGG CCTTTGCTGT 
CATCCTTTCT TCTCCAAAAT TGACTGGATT AACATTCGTA ACTCTCCTCC CCCCTTCGTT 
CCCACCCTCA AGTCTGATGA TGACACCTCC AATTTTGATG AACCAGAGAA GAATTCGTGG 
GTTTCATCCT CTCCGTGCCA GCTGAACCCC TCAGGTTTCT CGGGCGAAGA ACTGCCGTTT 
GTGGGGTTTT CGTATAGCAA GGCACTGGGA ATTCTTGGTA GATCTGAGCC TGTTGTATCA 
AGTCTGGACT CCCCTGCCAA GACTAGCTCC ATGGAAAAGA AACTCCTTAT CAAAAGCAAA 
GAGCTGCAGG ACTCCCAGGA CAAGTGTCAC AAGATGGAGC AGGAAATGAC CCGGTTACAT 
CGGAGAGTGT CAGAGGTGGA GGCTGTGCTT AGTCAGAAGG AGGTGGAGCT GAAGGCCTCT 
GAGACTCAGA GATCCCTCCT GGAGCAGGAC CTTGCTACCT ACATCACAGA ATGCAGTAGC 
TTAAAGCGAA GTTTGGAGCA AGCACGGATG GAGGTGTCCC AAGAGGACGA CAAAGCACTG 
CAGCTCCTCC ATGATATCAG AGAGCAGAGC CGGAAGCTCC AAGAAATCAA AGAGCAGGAG 
TACCAGGCTC AAGTGGAAGA AATGAGGTTG ATGATGAATC AGTTGGAAGA GGACCTGGTT 
TCGGCGAGAA GGCGGAGTGA CCTCTATGAA TCGGAGCTCA GAGAGTCTCG GCTGGCTGCT 
GAAGAGTTTA AGCGGAAAGC AACAGAATGT CAGCATAAAC TGATGAAGGC TAAAGATCAA 
GGGAAGCCTG AAGTGGGAGA ATATGCCAAA CTGGAGAAGA TCAATGCTGA GCAGCAGCTC 
AAAATTCAGG AGCTCCAAGA GAAGCTAGAA AAGGCTGTGA AAGCTAGCAC AGAGGCCACC 
GAGCTGCTGC AGAACATCCG TCAGGCGAAG GAGCGGGCCG AGCGTGAGCT GGAGAAGCTG 
CACAACCGTG AGGACTCCTC CGAGGGCATC AAGAAGAAGC TGGTGGAGGC CGAGGAACGC 
CGCCACTCTC TGGAGAACAA GGTAAAGAGG CTAGAGACCA TGGAGCGTAG AGAAAACAGA 
CTGAAGGATG ACATCCAGAC AAAGTCCCAA CAGATCCAGC AGATGGCTGA TAAAATTCTG 
GAGCTGGAGG AGAAGCACAG AGAAGCCCAG GTCTCAGCGC AGCATCTGGA GGTGCACCTG 
AAACAGAAAG AGCAGCACTA TGAAGAAAAA ATTAAAGTGT TGGACAATCA GATAAAGAAA 
GACCTAGCAG ACAAGGAGAC ACTGGAGAAC CTGATGCAAA GACACGAAGA GGAGGCCCAT 
GAGAAAGGCA AAATTCTCAG CGAGCAGAAG GCGATGATCA ATGCTATGGA TTCCAAGATC 
AGATCCCTGG AACAAAGGAT AGTGGAATTG TCGGAAGCCA ATAAACTTGC GGCCAACAGC 
AGTCTCTTTA CCCAGAGGAA CATGAAGGCC CAGGAAGAGA TGATTTCAGA ACTCAGGCAA 
CAGAAGTTTT ACCTGGAGAC GCAAGCTGGG AAATTGGAGG CCCAGAACCG GAAGCTGGAG 
GAGCAGCTGG AAAAAATAAG CCACCAAGAC CACAGTGACA AGAACCGTCT GCTGGAGCTG 
GAGACCAGGC TAAGGGAGGT CAGTCTAGAA CATGAAGAGC AGAAACTGGA GCTCAAACGC 
CAGCTCACAG AGCTGCAGCT CTCCCTGCAG GAGCGTGAAT CCCAGCTGAC AGCCCTGCAG 
GCAGCACGGG CGGCCCTGGA GAGCCAGCTC CGCCAGGCCA AGACGGAGCT GGAAGAGACG 
ACAGCAGAAG CAGAAGAAGA GATCCAGGCG CTTACGGCAC ATAGAGATGA AATCCAGCGC 
AAATTCGATG CCCTTCGTAA CAGCTGTACT GTGATCACGG ACTTGGAGGA GCAGCTAAAC 
CAGCTGACGG AGGACAATGC CGAGCTCAAC AACCAAAACT TCTACTTGTC TAAACAACTC 
GATGAGGCCT CTGGTGCCAA CGACGAGATT GTGCAGCTGC GAAGTGAAGT GGACCATCTC 
CGCCGCGAGA TCACTGAGAG GGAGATGCAG CTCACTAGCC AGAAGCAAAC GATGGAGGCG 
CTGAAGACCA CGTGCACAAT GCTGGAGGAG CAGGTCATGG ACCTGGAGGC CCTGAACGAC 
GAGCTGCTGG AGAAGGAGCG GCAGTGGGAG GCCTGGAGGA GTGTCCTTGG TGACGAGAAG 
TCCCAGTTTG AGTGTCGGGT TCGAGAGTTG CAGAGGATGC TGGACACCGA AAAGCAGAGC 
AGGGCGAGGG CCGACCAGAG GATCACTGAG TCGCGCCAGG TGGTCGAGCT GGCAGTAAAG 
GAGCACAAGG CCGAGATCCT GGCCTTGCAG CAGGCTCTCA AGGAGCAGAA GCTGAAGGCC 
GAGAGCCTCT CCGACAAGCT CAATGATCTG GAGAAGAAGC ACGCCATGCT TGAAATGAAT 
GCCCGAAGTT TACAACAGAA GCTGGAGACA GAACGAGAGC TCAAACAAAG GCTTCTGGAA 
GAGCAAGCCA AGTTACAGCA GCAGATGGAC CTGCAGAAGA ATCATATTTT CCGTCTGACT 
CAAGGGCTGC AAGAAGCTCT GGATCGGGCT GATCTGCTGA AGACAGAAAG GAGTGACCTG 
GAATATCAGC TGGAAAACAT TCAGGTTCTC TATTCTCATG AGAAGGTGAA GATGGAAGGC 
ACTATTTCCC AGCAAACCAA ACTCATCGAT TTTCTGCAAG CCAAAATGGA CCAGCCTGCC 
AAAAAGAAAA AGGGTTTATT TAGTCGACGG AAAGAGGACC CTGCTTTGCC CACACAGGTT 
CCTCTGCAGT ACAATGAGCT GAAGGTGGCT CTGGAGAAGG AGAAAGCTCG CTGCGCGGAG 
CTCGAGGAAG CCCTGCAGAA GACCCGCATC GAGCTCCGCT CCGCCCGGGA GGAAGCTGCC 
CACCGGAAAG CTGCAGACCA CCCACACCCA TCTACGCCGG CTACCGCCAG GCAGCAGATC 
GCCATGTCCG CCATTGTGCG GTCACCCGAG CACCAGCCCA GCGCCCTGAG CCTGCTCGCC 
CCGCCGTCCA GCCGCAGGAA GGAGTCTTCC ACTCCAGAGG AATTCAGCCG GCGTCTTAAG 
GAGCGCATGC ACCACAACAT CCCTCACCGG TTCAACGTGG GACTGAACAT GCGAGCCACG 
AAGTGCGCCG TGTGTCTGGA TACCGTGCAC TTCGGGCGCC AGGCATCCAA GTGTCTCGAA 
TGTCAGGTCA TGTGCCACCC CAAGTGCTCC ACATGCTTGC CAGCCACCTG TGGCCTGCCA 
GCCGAATACG CCACACACTT CACCGAGGCC TTCTGCCGCG ACAAAATGAA CTCCCCGGGT 
CTGCAGTCCA AGGAGCCCAG CAGCAGCCTG CACCTGGAAG GGTGGATGAA GGTGCCCAGG 
AATAACAAGC GAGGACAGCA AGGCTGGGAC AGGAAGTACA TCGTTCTGGA GGGCTCCAAA 
GTCCTCATTT ATGACAACGA AGCCAGAGAA GCTGGACAGA GGCCGGTGGA AGAATTTGAG 
CTGTGCCTTC CCGACGGGGA CGTGTCTATT CATGGCGCCG TTGGTGCTTC TGAACTTGCA 
AACACAGCCA AAGCAGATGT CCCCTATGTC CTGAAGATGG AGTCTCACCC CCATACCACC 
TGCTGGCCTG GGAGGACCCT CTACTTGCTG GCTCCCAGCT TTCCCGACAA GCAGCGCTGG 
GTCACCGCCT TAGAGTCAGT TGTGGCAGGT GGGAGAGTGT CTAGGGAAAA GGCGGAAGCC 
GACGCTAAAT TGCTCGGAAA CTCCCTGTTG AAACTGGAAG GTGATGACCG TCTGGACATG 
AACTGCACGC TGCCCTTCAG CGACCAGGTG GTGCTGGTGG GCACCGAGGA AGGGCTGTAT 
GCGCTGAATG TCTTGAAGAA CTCCCTCACC CACGTCCCAG GAATCGGAGC AGTCTTCCAG 
ATTTACATCA TCAAGGACCT GGAGAAGCTC CTCATGATCG CAGGAGAGGA GCGGGCCCTG 
TGTCTCGTGG ATGTGAAGAA GGTGAAGCAG TCCCTCGCGC AGTCTCACCT CCCCGCCCAG 
CCAGACATCT CGCCCAACAT CTTTGAAGCG GTCAAGGGCT GCCACTTGTT TGCTGCTGGC 
AAGATTGAGA GCGGGCTCTG CATCTGTGCA GCCATGCCCA GCAAAGTTGT CATTCTCCGC 
TACAATGAAA ACCTCAGCAA GTACTGCATT CGCAAAGAGA TCGAGACCTC GGAGCCCTGC 
AGCTGTATCC ACTTCACCAA CTACAGTATC CTCATCGGAA CCAATAAATT CTACGAAATC 
GACATGAAGC AGTACACGCT AGAGGAATTC CTGGATAAGA ACGACCATTC CTTGGCGCCT 
GCCGTGTTTG CCTCCTCTTC CAACAGCTTC CCCGTGTCCA TCATGCAGGT GAACGGCGCG 
GGGCAGCGGG AGGAGTTCCT GCTCTGTTTC CACGAATTCG GGGTGTTCGT GGATTCTTAT 
GGAAGACGCA GCCGCACAGA TGATCTCAAG TGGAGTCGCT TACCTCTGGC CTTTGCCTAC 
AGAGAACCCT ATCTGTTTGT GACTCACTTC AACTCACTCG AAGTAATTGA GATCCAGGCC 
CGCTCCTCTC TGGGGACCCC CGCCCGAGCA TATTTGGAAA TCCCCAACCC ACGCTACCTG 
GGCCCTGCAA TCTCCTCAGG AGCCATCTAC CTGGCGTCCT CATATCAGGA TAAGTTAAGG 
GTCATCTGCT GCAAAGGAAA CCTGGTGAAG GAGACCGGCA CCGACCACCA CCGTGGCCCA 
TCCACCTCCC GCAGCCCCAA CAAGCGAGGC CCGCCGACGT ACAACGAGCA CATCACCAAG 
CGCGTGGCCT CAAGCCCGGC GCCTCCCGAA GGCCCCAGCC ACCCTCGAGA GCCAAGCACA 
CCCCACCGCT ACCGCGAGGG GCGGACCGAG CTGCGCAGGG ACAAGTCTCC GGGCCGCCCC 
CTGGAGCGCG AGAAGTCCCC CGGCCGGATG CTGAGCACGC GGAGGGAGCG CTCCCCCGGG 
CGGCTGTTTG AGGACAGCAG CAGGGGCCGG CTGCCCGTGG GGGCCGTGAG GACCCCGCTG 
TCCCAGGTCA ACAAGGTCTG GGACCAGTCT TCAGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CIT ( XM_015467110.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015322596.1 Bos taurus citron rho-interacting serine/threonine kinase (CIT), mRNA.

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CloneID	OBa93236
Clone ID Related Accession (Same CDS sequence)	XM_015475534.1 , XM_015467110.1
Accession Version	XM_015467110.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5979bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	citron Rho-interacting kinase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646371.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

ATGTTGAAGT TCAAGTATGG AGCACGGAAT CTACTGGACG CTGGTACTGT TGAATCCATT 
GCCAGCCGGG CCTCCAGGCT GAATCTTTTC TTCCAGGGGA AACCACCCTC CATGACTCAA 
CAGCAGATGT CTCTTCTTTC CCGAGAAGCG ATATTAGATG CCCTCTTTGT TCTCTTTAAA 
GAATGCAGTC AGCCAGCTCT GATGAAGATT AAGCACGTGA GCAACTTTGT CCAAAAGTGT 
AAGTGTGGGG AGAACTTTTT CTTGAAAATT GTCCTGCTTA GTGAGAATGT CATGGAATAT 
CAGCCTGGAG GGGATTTGCT GTCACTTTTG AATAGATATG AGGACCAATT AGATGAAAAT 
ATGATTCAGT TTTACCTAGC TGAACTGATT TTGGCTGTCC ACAGCGTTCA TCAGATGGGC 
TATGTACATC GAGACATCAA GCCCGAGAAC ATCCTCATTG ACCGAACGGG ACACATCAAG 
CTGGTGGATT TTGGATCAGC TGCTAAAATG AACTCAAATA AGATGGTAAA TGCCAAACTC 
CCGGTTGGTA CTCCAGATTA CATGGCCCCT GAAGTGTTGA CTGTGATGAA TGGGGATGGA 
AAAGGTGCCT ATAGCCTAGA CTGTGATTGG TGGTCAGTGG GAGTTATTGC TTATGAGATG 
GTTTATGGAA GATGCCCATT CACTGAAGGA ACCTCAGCCA AAACCTTCAA TAACATCATG 
AATTTCCAGC GGTTCTTGAA GTTTCCAGAT GACCCCAAAG TTAGCAGTGA ATTGCTTGAT 
CTAATCCAGA GTTTGTTGTG TGGCCAGAAA GAGAGACTGA AGTTTGAGGG CCTTTGCTGT 
CATCCTTTCT TCTCCAAAAT TGACTGGATT AACATTCGTA ACTCTCCTCC CCCCTTCGTT 
CCCACCCTCA AGTCTGATGA TGACACCTCC AATTTTGATG AACCAGAGAA GAATTCGTGG 
GTTTCATCCT CTCCGTGCCA GCTGAACCCC TCAGGTTTCT CGGGCGAAGA ACTGCCGTTT 
GTGGGGTTTT CGTATAGCAA GGCACTGGGA ATTCTTGGTA GATCTGAGCC TGTTGTATCA 
AGTCTGGACT CCCCTGCCAA GACTAGCTCC ATGGAAAAGA AACTCCTTAT CAAAAGCAAA 
GAGCTGCAGG ACTCCCAGGA CAAGTGTCAC AAGATGGAGC AGGAAATGAC CCGGTTACAT 
CGGAGAGTGT CAGAGGTGGA GGCTGTGCTT AGTCAGAAGG AGGTGGAGCT GAAGGCCTCT 
GAGACTCAGA GATCCCTCCT GGAGCAGGAC CTTGCTACCT ACATCACAGA ATGCAGTAGC 
TTAAAGCGAA GTTTGGAGCA AGCACGGATG GAGGTGTCCC AAGAGGACGA CAAAGCACTG 
CAGCTCCTCC ATGATATCAG AGAGCAGAGC CGGAAGCTCC AAGAAATCAA AGAGCAGGAG 
TACCAGGCTC AAGTGGAAGA AATGAGGTTG ATGATGAATC AGTTGGAAGA GGACCTGGTT 
TCGGCGAGAA GGCGGAGTGA CCTCTATGAA TCGGAGCTCA GAGAGTCTCG GCTGGCTGCT 
GAAGAGTTTA AGCGGAAAGC AACAGAATGT CAGCATAAAC TGATGAAGGC TAAAGATCAA 
GGGAAGCCTG AAGTGGGAGA ATATGCCAAA CTGGAGAAGA TCAATGCTGA GCAGCAGCTC 
AAAATTCAGG AGCTCCAAGA GAAGCTAGAA AAGGCTGTGA AAGCTAGCAC AGAGGCCACC 
GAGCTGCTGC AGAACATCCG TCAGGCGAAG GAGCGGGCCG AGCGTGAGCT GGAGAAGCTG 
CACAACCGTG AGGACTCCTC CGAGGGCATC AAGAAGAAGC TGGTGGAGGC CGAGGAACGC 
CGCCACTCTC TGGAGAACAA GGTAAAGAGG CTAGAGACCA TGGAGCGTAG AGAAAACAGA 
CTGAAGGATG ACATCCAGAC AAAGTCCCAA CAGATCCAGC AGATGGCTGA TAAAATTCTG 
GAGCTGGAGG AGAAGCACAG AGAAGCCCAG GTCTCAGCGC AGCATCTGGA GGTGCACCTG 
AAACAGAAAG AGCAGCACTA TGAAGAAAAA ATTAAAGTGT TGGACAATCA GATAAAGAAA 
GACCTAGCAG ACAAGGAGAC ACTGGAGAAC CTGATGCAAA GACACGAAGA GGAGGCCCAT 
GAGAAAGGCA AAATTCTCAG CGAGCAGAAG GCGATGATCA ATGCTATGGA TTCCAAGATC 
AGATCCCTGG AACAAAGGAT AGTGGAATTG TCGGAAGCCA ATAAACTTGC GGCCAACAGC 
AGTCTCTTTA CCCAGAGGAA CATGAAGGCC CAGGAAGAGA TGATTTCAGA ACTCAGGCAA 
CAGAAGTTTT ACCTGGAGAC GCAAGCTGGG AAATTGGAGG CCCAGAACCG GAAGCTGGAG 
GAGCAGCTGG AAAAAATAAG CCACCAAGAC CACAGTGACA AGAACCGTCT GCTGGAGCTG 
GAGACCAGGC TAAGGGAGGT CAGTCTAGAA CATGAAGAGC AGAAACTGGA GCTCAAACGC 
CAGCTCACAG AGCTGCAGCT CTCCCTGCAG GAGCGTGAAT CCCAGCTGAC AGCCCTGCAG 
GCAGCACGGG CGGCCCTGGA GAGCCAGCTC CGCCAGGCCA AGACGGAGCT GGAAGAGACG 
ACAGCAGAAG CAGAAGAAGA GATCCAGGCG CTTACGGCAC ATAGAGATGA AATCCAGCGC 
AAATTCGATG CCCTTCGTAA CAGCTGTACT GTGATCACGG ACTTGGAGGA GCAGCTAAAC 
CAGCTGACGG AGGACAATGC CGAGCTCAAC AACCAAAACT TCTACTTGTC TAAACAACTC 
GATGAGGCCT CTGGTGCCAA CGACGAGATT GTGCAGCTGC GAAGTGAAGT GGACCATCTC 
CGCCGCGAGA TCACTGAGAG GGAGATGCAG CTCACTAGCC AGAAGCAAAC GATGGAGGCG 
CTGAAGACCA CGTGCACAAT GCTGGAGGAG CAGGTCATGG ACCTGGAGGC CCTGAACGAC 
GAGCTGCTGG AGAAGGAGCG GCAGTGGGAG GCCTGGAGGA GTGTCCTTGG TGACGAGAAG 
TCCCAGTTTG AGTGTCGGGT TCGAGAGTTG CAGAGGATGC TGGACACCGA AAAGCAGAGC 
AGGGCGAGGG CCGACCAGAG GATCACTGAG TCGCGCCAGG TGGTCGAGCT GGCAGTAAAG 
GAGCACAAGG CCGAGATCCT GGCCTTGCAG CAGGCTCTCA AGGAGCAGAA GCTGAAGGCC 
GAGAGCCTCT CCGACAAGCT CAATGATCTG GAGAAGAAGC ACGCCATGCT TGAAATGAAT 
GCCCGAAGTT TACAACAGAA GCTGGAGACA GAACGAGAGC TCAAACAAAG GCTTCTGGAA 
GAGCAAGCCA AGTTACAGCA GCAGATGGAC CTGCAGAAGA ATCATATTTT CCGTCTGACT 
CAAGGGCTGC AAGAAGCTCT GGATCGGGCT GATCTGCTGA AGACAGAAAG GAGTGACCTG 
GAATATCAGC TGGAAAACAT TCAGGTTCTC TATTCTCATG AGAAGGTGAA GATGGAAGGC 
ACTATTTCCC AGCAAACCAA ACTCATCGAT TTTCTGCAAG CCAAAATGGA CCAGCCTGCC 
AAAAAGAAAA AGGGTTTATT TAGTCGACGG AAAGAGGACC CTGCTTTGCC CACACAGGTT 
CCTCTGCAGT ACAATGAGCT GAAGGTGGCT CTGGAGAAGG AGAAAGCTCG CTGCGCGGAG 
CTCGAGGAAG CCCTGCAGAA GACCCGCATC GAGCTCCGCT CCGCCCGGGA GGAAGCTGCC 
CACCGGAAAG CTGCAGACCA CCCACACCCA TCTACGCCGG CTACCGCCAG GCAGCAGATC 
GCCATGTCCG CCATTGTGCG GTCACCCGAG CACCAGCCCA GCGCCCTGAG CCTGCTCGCC 
CCGCCGTCCA GCCGCAGGAA GGAGTCTTCC ACTCCAGAGG AATTCAGCCG GCGTCTTAAG 
GAGCGCATGC ACCACAACAT CCCTCACCGG TTCAACGTGG GACTGAACAT GCGAGCCACG 
AAGTGCGCCG TGTGTCTGGA TACCGTGCAC TTCGGGCGCC AGGCATCCAA GTGTCTCGAA 
TGTCAGGTCA TGTGCCACCC CAAGTGCTCC ACATGCTTGC CAGCCACCTG TGGCCTGCCA 
GCCGAATACG CCACACACTT CACCGAGGCC TTCTGCCGCG ACAAAATGAA CTCCCCGGGT 
CTGCAGTCCA AGGAGCCCAG CAGCAGCCTG CACCTGGAAG GGTGGATGAA GGTGCCCAGG 
AATAACAAGC GAGGACAGCA AGGCTGGGAC AGGAAGTACA TCGTTCTGGA GGGCTCCAAA 
GTCCTCATTT ATGACAACGA AGCCAGAGAA GCTGGACAGA GGCCGGTGGA AGAATTTGAG 
CTGTGCCTTC CCGACGGGGA CGTGTCTATT CATGGCGCCG TTGGTGCTTC TGAACTTGCA 
AACACAGCCA AAGCAGATGT CCCCTATGTC CTGAAGATGG AGTCTCACCC CCATACCACC 
TGCTGGCCTG GGAGGACCCT CTACTTGCTG GCTCCCAGCT TTCCCGACAA GCAGCGCTGG 
GTCACCGCCT TAGAGTCAGT TGTGGCAGGT GGGAGAGTGT CTAGGGAAAA GGCGGAAGCC 
GACGCTAAAT TGCTCGGAAA CTCCCTGTTG AAACTGGAAG GTGATGACCG TCTGGACATG 
AACTGCACGC TGCCCTTCAG CGACCAGGTG GTGCTGGTGG GCACCGAGGA AGGGCTGTAT 
GCGCTGAATG TCTTGAAGAA CTCCCTCACC CACGTCCCAG GAATCGGAGC AGTCTTCCAG 
ATTTACATCA TCAAGGACCT GGAGAAGCTC CTCATGATCG CAGGAGAGGA GCGGGCCCTG 
TGTCTCGTGG ATGTGAAGAA GGTGAAGCAG TCCCTCGCGC AGTCTCACCT CCCCGCCCAG 
CCAGACATCT CGCCCAACAT CTTTGAAGCG GTCAAGGGCT GCCACTTGTT TGCTGCTGGC 
AAGATTGAGA GCGGGCTCTG CATCTGTGCA GCCATGCCCA GCAAAGTTGT CATTCTCCGC 
TACAATGAAA ACCTCAGCAA GTACTGCATT CGCAAAGAGA TCGAGACCTC GGAGCCCTGC 
AGCTGTATCC ACTTCACCAA CTACAGTATC CTCATCGGAA CCAATAAATT CTACGAAATC 
GACATGAAGC AGTACACGCT AGAGGAATTC CTGGATAAGA ACGACCATTC CTTGGCGCCT 
GCCGTGTTTG CCTCCTCTTC CAACAGCTTC CCCGTGTCCA TCATGCAGGT GAACGGCGCG 
GGGCAGCGGG AGGAGTTCCT GCTCTGTTTC CACGAATTCG GGGTGTTCGT GGATTCTTAT 
GGAAGACGCA GCCGCACAGA TGATCTCAAG TGGAGTCGCT TACCTCTGGC CTTTGCCTAC 
AGAGAACCCT ATCTGTTTGT GACTCACTTC AACTCACTCG AAGTAATTGA GATCCAGGCC 
CGCTCCTCTC TGGGGACCCC CGCCCGAGCA TATTTGGAAA TCCCCAACCC ACGCTACCTG 
GGCCCTGCAA TCTCCTCAGG AGCCATCTAC CTGGCGTCCT CATATCAGGA TAAGTTAAGG 
GTCATCTGCT GCAAAGGAAA CCTGGTGAAG GAGACCGGCA CCGACCACCA CCGTGGCCCA 
TCCACCTCCC GCAGCCCCAA CAAGCGAGGC CCGCCGACGT ACAACGAGCA CATCACCAAG 
CGCGTGGCCT CAAGCCCGGC GCCTCCCGAA GGCCCCAGCC ACCCTCGAGA GCCAAGCACA 
CCCCACCGCT ACCGCGAGGG GCGGACCGAG CTGCGCAGGG ACAAGTCTCC GGGCCGCCCC 
CTGGAGCGCG AGAAGTCCCC CGGCCGGATG CTGAGCACGC GGAGGGAGCG CTCCCCCGGG 
CGGCTGTTTG AGGACAGCAG CAGGGGCCGG CTGCCCGTGG GGGCCGTGAG GACCCCGCTG 
TCCCAGGTCA ACAAGGTCTG GGACCAGTCT TCAGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015322596.1
CDS	263..6241
Translation	MLKFKYGARNLLDAGTVESIASRASRLNLFFQGKPPSMTQQQMSLLSREAILDALFVLFKECSQPALMKIKHVSNFVQKCKCGENFFLKIVLLSENVMEYQPGGDLLSLLNRYEDQLDENMIQFYLAELILAVHSVHQMGYVHRDIKPENILIDRTGHIKLVDFGSAAKMNSNKMVNAKLPVGTPDYMAPEVLTVMNGDGKGAYSLDCDWWSVGVIAYEMVYGRCPFTEGTSAKTFNNIMNFQRFLKFPDDPKVSSELLDLIQSLLCGQKERLKFEGLCCHPFFSKIDWINIRNSPPPFVPTLKSDDDTSNFDEPEKNSWVSSSPCQLNPSGFSGEELPFVGFSYSKALGILGRSEPVVSSLDSPAKTSSMEKKLLIKSKELQDSQDKCHKMEQEMTRLHRRVSEVEAVLSQKEVELKASETQRSLLEQDLATYITECSSLKRSLEQARMEVSQEDDKALQLLHDIREQSRKLQEIKEQEYQAQVEEMRLMMNQLEEDLVSARRRSDLYESELRESRLAAEEFKRKATECQHKLMKAKDQGKPEVGEYAKLEKINAEQQLKIQELQEKLEKAVKASTEATELLQNIRQAKERAERELEKLHNREDSSEGIKKKLVEAEERRHSLENKVKRLETMERRENRLKDDIQTKSQQIQQMADKILELEEKHREAQVSAQHLEVHLKQKEQHYEEKIKVLDNQIKKDLADKETLENLMQRHEEEAHEKGKILSEQKAMINAMDSKIRSLEQRIVELSEANKLAANSSLFTQRNMKAQEEMISELRQQKFYLETQAGKLEAQNRKLEEQLEKISHQDHSDKNRLLELETRLREVSLEHEEQKLELKRQLTELQLSLQERESQLTALQAARAALESQLRQAKTELEETTAEAEEEIQALTAHRDEIQRKFDALRNSCTVITDLEEQLNQLTEDNAELNNQNFYLSKQLDEASGANDEIVQLRSEVDHLRREITEREMQLTSQKQTMEALKTTCTMLEEQVMDLEALNDELLEKERQWEAWRSVLGDEKSQFECRVRELQRMLDTEKQSRARADQRITESRQVVELAVKEHKAEILALQQALKEQKLKAESLSDKLNDLEKKHAMLEMNARSLQQKLETERELKQRLLEEQAKLQQQMDLQKNHIFRLTQGLQEALDRADLLKTERSDLEYQLENIQVLYSHEKVKMEGTISQQTKLIDFLQAKMDQPAKKKKGLFSRRKEDPALPTQVPLQYNELKVALEKEKARCAELEEALQKTRIELRSAREEAAHRKAADHPHPSTPATARQQIAMSAIVRSPEHQPSALSLLAPPSSRRKESSTPEEFSRRLKERMHHNIPHRFNVGLNMRATKCAVCLDTVHFGRQASKCLECQVMCHPKCSTCLPATCGLPAEYATHFTEAFCRDKMNSPGLQSKEPSSSLHLEGWMKVPRNNKRGQQGWDRKYIVLEGSKVLIYDNEAREAGQRPVEEFELCLPDGDVSIHGAVGASELANTAKADVPYVLKMESHPHTTCWPGRTLYLLAPSFPDKQRWVTALESVVAGGRVSREKAEADAKLLGNSLLKLEGDDRLDMNCTLPFSDQVVLVGTEEGLYALNVLKNSLTHVPGIGAVFQIYIIKDLEKLLMIAGEERALCLVDVKKVKQSLAQSHLPAQPDISPNIFEAVKGCHLFAAGKIESGLCICAAMPSKVVILRYNENLSKYCIRKEIETSEPCSCIHFTNYSILIGTNKFYEIDMKQYTLEEFLDKNDHSLAPAVFASSSNSFPVSIMQVNGAGQREEFLLCFHEFGVFVDSYGRRSRTDDLKWSRLPLAFAYREPYLFVTHFNSLEVIEIQARSSLGTPARAYLEIPNPRYLGPAISSGAIYLASSYQDKLRVICCKGNLVKETGTDHHRGPSTSRSPNKRGPPTYNEHITKRVASSPAPPEGPSHPREPSTPHRYREGRTELRRDKSPGRPLEREKSPGRMLSTRRERSPGRLFEDSSRGRLPVGAVRTPLSQVNKVWDQSSV

Target ORF information:

RefSeq Version	XM_015467110.1
Organism	Bos taurus(cattle)
Definition	Bos taurus citron rho-interacting serine/threonine kinase (CIT), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015467110.1

ORF Insert Sequence:

ATGTTGAAGT TCAAGTATGG AGCACGGAAT CTACTGGACG CTGGTACTGT TGAATCCATT 
GCCAGCCGGG CCTCCAGGCT GAATCTTTTC TTCCAGGGGA AACCACCCTC CATGACTCAA 
CAGCAGATGT CTCTTCTTTC CCGAGAAGCG ATATTAGATG CCCTCTTTGT TCTCTTTAAA 
GAATGCAGTC AGCCAGCTCT GATGAAGATT AAGCACGTGA GCAACTTTGT CCAAAAGTGT 
AAGTGTGGGG AGAACTTTTT CTTGAAAATT GTCCTGCTTA GTGAGAATGT CATGGAATAT 
CAGCCTGGAG GGGATTTGCT GTCACTTTTG AATAGATATG AGGACCAATT AGATGAAAAT 
ATGATTCAGT TTTACCTAGC TGAACTGATT TTGGCTGTCC ACAGCGTTCA TCAGATGGGC 
TATGTACATC GAGACATCAA GCCCGAGAAC ATCCTCATTG ACCGAACGGG ACACATCAAG 
CTGGTGGATT TTGGATCAGC TGCTAAAATG AACTCAAATA AGATGGTAAA TGCCAAACTC 
CCGGTTGGTA CTCCAGATTA CATGGCCCCT GAAGTGTTGA CTGTGATGAA TGGGGATGGA 
AAAGGTGCCT ATAGCCTAGA CTGTGATTGG TGGTCAGTGG GAGTTATTGC TTATGAGATG 
GTTTATGGAA GATGCCCATT CACTGAAGGA ACCTCAGCCA AAACCTTCAA TAACATCATG 
AATTTCCAGC GGTTCTTGAA GTTTCCAGAT GACCCCAAAG TTAGCAGTGA ATTGCTTGAT 
CTAATCCAGA GTTTGTTGTG TGGCCAGAAA GAGAGACTGA AGTTTGAGGG CCTTTGCTGT 
CATCCTTTCT TCTCCAAAAT TGACTGGATT AACATTCGTA ACTCTCCTCC CCCCTTCGTT 
CCCACCCTCA AGTCTGATGA TGACACCTCC AATTTTGATG AACCAGAGAA GAATTCGTGG 
GTTTCATCCT CTCCGTGCCA GCTGAACCCC TCAGGTTTCT CGGGCGAAGA ACTGCCGTTT 
GTGGGGTTTT CGTATAGCAA GGCACTGGGA ATTCTTGGTA GATCTGAGCC TGTTGTATCA 
AGTCTGGACT CCCCTGCCAA GACTAGCTCC ATGGAAAAGA AACTCCTTAT CAAAAGCAAA 
GAGCTGCAGG ACTCCCAGGA CAAGTGTCAC AAGATGGAGC AGGAAATGAC CCGGTTACAT 
CGGAGAGTGT CAGAGGTGGA GGCTGTGCTT AGTCAGAAGG AGGTGGAGCT GAAGGCCTCT 
GAGACTCAGA GATCCCTCCT GGAGCAGGAC CTTGCTACCT ACATCACAGA ATGCAGTAGC 
TTAAAGCGAA GTTTGGAGCA AGCACGGATG GAGGTGTCCC AAGAGGACGA CAAAGCACTG 
CAGCTCCTCC ATGATATCAG AGAGCAGAGC CGGAAGCTCC AAGAAATCAA AGAGCAGGAG 
TACCAGGCTC AAGTGGAAGA AATGAGGTTG ATGATGAATC AGTTGGAAGA GGACCTGGTT 
TCGGCGAGAA GGCGGAGTGA CCTCTATGAA TCGGAGCTCA GAGAGTCTCG GCTGGCTGCT 
GAAGAGTTTA AGCGGAAAGC AACAGAATGT CAGCATAAAC TGATGAAGGC TAAAGATCAA 
GGGAAGCCTG AAGTGGGAGA ATATGCCAAA CTGGAGAAGA TCAATGCTGA GCAGCAGCTC 
AAAATTCAGG AGCTCCAAGA GAAGCTAGAA AAGGCTGTGA AAGCTAGCAC AGAGGCCACC 
GAGCTGCTGC AGAACATCCG TCAGGCGAAG GAGCGGGCCG AGCGTGAGCT GGAGAAGCTG 
CACAACCGTG AGGACTCCTC CGAGGGCATC AAGAAGAAGC TGGTGGAGGC CGAGGAACGC 
CGCCACTCTC TGGAGAACAA GGTAAAGAGG CTAGAGACCA TGGAGCGTAG AGAAAACAGA 
CTGAAGGATG ACATCCAGAC AAAGTCCCAA CAGATCCAGC AGATGGCTGA TAAAATTCTG 
GAGCTGGAGG AGAAGCACAG AGAAGCCCAG GTCTCAGCGC AGCATCTGGA GGTGCACCTG 
AAACAGAAAG AGCAGCACTA TGAAGAAAAA ATTAAAGTGT TGGACAATCA GATAAAGAAA 
GACCTAGCAG ACAAGGAGAC ACTGGAGAAC CTGATGCAAA GACACGAAGA GGAGGCCCAT 
GAGAAAGGCA AAATTCTCAG CGAGCAGAAG GCGATGATCA ATGCTATGGA TTCCAAGATC 
AGATCCCTGG AACAAAGGAT AGTGGAATTG TCGGAAGCCA ATAAACTTGC GGCCAACAGC 
AGTCTCTTTA CCCAGAGGAA CATGAAGGCC CAGGAAGAGA TGATTTCAGA ACTCAGGCAA 
CAGAAGTTTT ACCTGGAGAC GCAAGCTGGG AAATTGGAGG CCCAGAACCG GAAGCTGGAG 
GAGCAGCTGG AAAAAATAAG CCACCAAGAC CACAGTGACA AGAACCGTCT GCTGGAGCTG 
GAGACCAGGC TAAGGGAGGT CAGTCTAGAA CATGAAGAGC AGAAACTGGA GCTCAAACGC 
CAGCTCACAG AGCTGCAGCT CTCCCTGCAG GAGCGTGAAT CCCAGCTGAC AGCCCTGCAG 
GCAGCACGGG CGGCCCTGGA GAGCCAGCTC CGCCAGGCCA AGACGGAGCT GGAAGAGACG 
ACAGCAGAAG CAGAAGAAGA GATCCAGGCG CTTACGGCAC ATAGAGATGA AATCCAGCGC 
AAATTCGATG CCCTTCGTAA CAGCTGTACT GTGATCACGG ACTTGGAGGA GCAGCTAAAC 
CAGCTGACGG AGGACAATGC CGAGCTCAAC AACCAAAACT TCTACTTGTC TAAACAACTC 
GATGAGGCCT CTGGTGCCAA CGACGAGATT GTGCAGCTGC GAAGTGAAGT GGACCATCTC 
CGCCGCGAGA TCACTGAGAG GGAGATGCAG CTCACTAGCC AGAAGCAAAC GATGGAGGCG 
CTGAAGACCA CGTGCACAAT GCTGGAGGAG CAGGTCATGG ACCTGGAGGC CCTGAACGAC 
GAGCTGCTGG AGAAGGAGCG GCAGTGGGAG GCCTGGAGGA GTGTCCTTGG TGACGAGAAG 
TCCCAGTTTG AGTGTCGGGT TCGAGAGTTG CAGAGGATGC TGGACACCGA AAAGCAGAGC 
AGGGCGAGGG CCGACCAGAG GATCACTGAG TCGCGCCAGG TGGTCGAGCT GGCAGTAAAG 
GAGCACAAGG CCGAGATCCT GGCCTTGCAG CAGGCTCTCA AGGAGCAGAA GCTGAAGGCC 
GAGAGCCTCT CCGACAAGCT CAATGATCTG GAGAAGAAGC ACGCCATGCT TGAAATGAAT 
GCCCGAAGTT TACAACAGAA GCTGGAGACA GAACGAGAGC TCAAACAAAG GCTTCTGGAA 
GAGCAAGCCA AGTTACAGCA GCAGATGGAC CTGCAGAAGA ATCATATTTT CCGTCTGACT 
CAAGGGCTGC AAGAAGCTCT GGATCGGGCT GATCTGCTGA AGACAGAAAG GAGTGACCTG 
GAATATCAGC TGGAAAACAT TCAGGTTCTC TATTCTCATG AGAAGGTGAA GATGGAAGGC 
ACTATTTCCC AGCAAACCAA ACTCATCGAT TTTCTGCAAG CCAAAATGGA CCAGCCTGCC 
AAAAAGAAAA AGGGTTTATT TAGTCGACGG AAAGAGGACC CTGCTTTGCC CACACAGGTT 
CCTCTGCAGT ACAATGAGCT GAAGGTGGCT CTGGAGAAGG AGAAAGCTCG CTGCGCGGAG 
CTCGAGGAAG CCCTGCAGAA GACCCGCATC GAGCTCCGCT CCGCCCGGGA GGAAGCTGCC 
CACCGGAAAG CTGCAGACCA CCCACACCCA TCTACGCCGG CTACCGCCAG GCAGCAGATC 
GCCATGTCCG CCATTGTGCG GTCACCCGAG CACCAGCCCA GCGCCCTGAG CCTGCTCGCC 
CCGCCGTCCA GCCGCAGGAA GGAGTCTTCC ACTCCAGAGG AATTCAGCCG GCGTCTTAAG 
GAGCGCATGC ACCACAACAT CCCTCACCGG TTCAACGTGG GACTGAACAT GCGAGCCACG 
AAGTGCGCCG TGTGTCTGGA TACCGTGCAC TTCGGGCGCC AGGCATCCAA GTGTCTCGAA 
TGTCAGGTCA TGTGCCACCC CAAGTGCTCC ACATGCTTGC CAGCCACCTG TGGCCTGCCA 
GCCGAATACG CCACACACTT CACCGAGGCC TTCTGCCGCG ACAAAATGAA CTCCCCGGGT 
CTGCAGTCCA AGGAGCCCAG CAGCAGCCTG CACCTGGAAG GGTGGATGAA GGTGCCCAGG 
AATAACAAGC GAGGACAGCA AGGCTGGGAC AGGAAGTACA TCGTTCTGGA GGGCTCCAAA 
GTCCTCATTT ATGACAACGA AGCCAGAGAA GCTGGACAGA GGCCGGTGGA AGAATTTGAG 
CTGTGCCTTC CCGACGGGGA CGTGTCTATT CATGGCGCCG TTGGTGCTTC TGAACTTGCA 
AACACAGCCA AAGCAGATGT CCCCTATGTC CTGAAGATGG AGTCTCACCC CCATACCACC 
TGCTGGCCTG GGAGGACCCT CTACTTGCTG GCTCCCAGCT TTCCCGACAA GCAGCGCTGG 
GTCACCGCCT TAGAGTCAGT TGTGGCAGGT GGGAGAGTGT CTAGGGAAAA GGCGGAAGCC 
GACGCTAAAT TGCTCGGAAA CTCCCTGTTG AAACTGGAAG GTGATGACCG TCTGGACATG 
AACTGCACGC TGCCCTTCAG CGACCAGGTG GTGCTGGTGG GCACCGAGGA AGGGCTGTAT 
GCGCTGAATG TCTTGAAGAA CTCCCTCACC CACGTCCCAG GAATCGGAGC AGTCTTCCAG 
ATTTACATCA TCAAGGACCT GGAGAAGCTC CTCATGATCG CAGGAGAGGA GCGGGCCCTG 
TGTCTCGTGG ATGTGAAGAA GGTGAAGCAG TCCCTCGCGC AGTCTCACCT CCCCGCCCAG 
CCAGACATCT CGCCCAACAT CTTTGAAGCG GTCAAGGGCT GCCACTTGTT TGCTGCTGGC 
AAGATTGAGA GCGGGCTCTG CATCTGTGCA GCCATGCCCA GCAAAGTTGT CATTCTCCGC 
TACAATGAAA ACCTCAGCAA GTACTGCATT CGCAAAGAGA TCGAGACCTC GGAGCCCTGC 
AGCTGTATCC ACTTCACCAA CTACAGTATC CTCATCGGAA CCAATAAATT CTACGAAATC 
GACATGAAGC AGTACACGCT AGAGGAATTC CTGGATAAGA ACGACCATTC CTTGGCGCCT 
GCCGTGTTTG CCTCCTCTTC CAACAGCTTC CCCGTGTCCA TCATGCAGGT GAACGGCGCG 
GGGCAGCGGG AGGAGTTCCT GCTCTGTTTC CACGAATTCG GGGTGTTCGT GGATTCTTAT 
GGAAGACGCA GCCGCACAGA TGATCTCAAG TGGAGTCGCT TACCTCTGGC CTTTGCCTAC 
AGAGAACCCT ATCTGTTTGT GACTCACTTC AACTCACTCG AAGTAATTGA GATCCAGGCC 
CGCTCCTCTC TGGGGACCCC CGCCCGAGCA TATTTGGAAA TCCCCAACCC ACGCTACCTG 
GGCCCTGCAA TCTCCTCAGG AGCCATCTAC CTGGCGTCCT CATATCAGGA TAAGTTAAGG 
GTCATCTGCT GCAAAGGAAA CCTGGTGAAG GAGACCGGCA CCGACCACCA CCGTGGCCCA 
TCCACCTCCC GCAGCCCCAA CAAGCGAGGC CCGCCGACGT ACAACGAGCA CATCACCAAG 
CGCGTGGCCT CAAGCCCGGC GCCTCCCGAA GGCCCCAGCC ACCCTCGAGA GCCAAGCACA 
CCCCACCGCT ACCGCGAGGG GCGGACCGAG CTGCGCAGGG ACAAGTCTCC GGGCCGCCCC 
CTGGAGCGCG AGAAGTCCCC CGGCCGGATG CTGAGCACGC GGAGGGAGCG CTCCCCCGGG 
CGGCTGTTTG AGGACAGCAG CAGGGGCCGG CTGCCCGTGG GGGCCGTGAG GACCCCGCTG 
TCCCAGGTCA ACAAGGTCTG GGACCAGTCT TCAGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CIT ( XM_024977733.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024833501.1 Bos taurus citron rho-interacting serine/threonine kinase (CIT), transcript variant X1, mRNA.

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CloneID	OBa224622
Clone ID Related Accession (Same CDS sequence)	XM_024977733.1 , XM_024977734.1
Accession Version	XM_024977733.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6252bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	citron Rho-interacting kinase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGAAGT TCAAGTATGG AGCACGGAAT CTACTGGACG CTGGTACTGT TGAATCCATT 
GCCAGCCGGG CCTCCAGGCT GAATCTTTTC TTCCAGGGGA AACCACCCTC CATGACTCAA 
CAGCAGATGT CTCTTCTTTC CCGAGAAGCG ATATTAGATG CCCTCTTTGT TCTCTTTAAA 
GAATGCAGTC AGCCAGCTCT GATGAAGATT AAGCACGTGA GCAACTTTGT CCAAAAGTAT 
TCCGATATCA TAGCTGAGTT GCAAGAGCTC CAGCCTTCAG TGAAGGACTT TGAAGTGAAA 
AGTGTCGTAG GCTGCGGTCA CTTTGCTGAA GTACAGGTGG TCAGAGAGAA AGCGACTGGG 
GATGTCTATG CCATGAAAGT CATGAAGAAG AAGGCCTTGT TGGCCCAGGA GCAGGTCTCA 
TATTTTGAGG AAGAACAGAA CATATTATCT CAGAGTACAA GTCCTTGGAT CCCCCAGTTG 
CAGTATGCCT TTCAGGACAA AAATAACCTT TATCTGGTCA TGGAATATCA GCCTGGAGGG 
GATTTGCTGT CACTTTTGAA TAGATATGAG GACCAATTAG ATGAAAATAT GATTCAGTTT 
TACCTAGCTG AACTGATTTT GGCTGTCCAC AGCGTTCATC AGATGGGCTA TGTACATCGA 
GACATCAAGC CCGAGAACAT CCTCATTGAC CGAACGGGAC ACATCAAGCT GGTGGATTTT 
GGATCAGCTG CTAAAATGAA CTCAAATAAG ATGGTAAATG CCAAACTCCC GGTTGGTACT 
CCAGATTACA TGGCCCCTGA AGTGTTGACT GTGATGAATG GGGATGGAAA AGGTGCCTAT 
AGCCTAGACT GTGATTGGTG GTCAGTGGGA GTTATTGCTT ATGAGATGGT TTATGGAAGA 
TGCCCATTCA CTGAAGGAAC CTCAGCCAAA ACCTTCAATA ACATCATGAA TTTCCAGCGG 
TTCTTGAAGT TTCCAGATGA CCCCAAAGTT AGCAGTGAAT TGCTTGATCT AATCCAGAGT 
TTGTTGTGTG GCCAGAAAGA GAGACTGAAG TTTGAGGGCC TTTGCTGTCA TCCTTTCTTC 
TCCAAAATTG ACTGGATTAA CATTCGTAAC TCTCCTCCCC CCTTCGTTCC CACCCTCAAG 
TCTGATGATG ACACCTCCAA TTTTGATGAA CCAGAGAAGA ATTCGTGGGT TTCATCCTCT 
CCGTGCCAGC TGAACCCCTC AGGTTTCTCG GGCGAAGAAC TGCCGTTTGT GGGGTTTTCG 
TATAGCAAGG CACTGGGAAT TCTTGGTAGA TCTGAGCCTG TTGTATCAAG TCTGGACTCC 
CCTGCCAAGA CTAGCTCCAT GGAAAAGAAA CTCCTTATCA AAAGCAAAGA GCTGCAGGAC 
TCCCAGGACA AGTGTCACAA GATGGAGCAG GAAATGACCC GGTTACATCG GAGAGTGTCA 
GAGGTGGAGG CTGTGCTTAG TCAGAAGGAG GTGGAGCTGA AGGCCTCTGA GACTCAGAGA 
TCCCTCCTGG AGCAGGACCT TGCTACCTAC ATCACAGAAT GCAGTAGCTT AAAGCGAAGT 
TTGGAGCAAG CACGGATGGA GGTGTCCCAA GAGGACGACA AAGCACTGCA GCTCCTCCAT 
GATATCAGAG AGCAGAGCCG GAAGCTCCAA GAAATCAAAG AGCAGGAGTA CCAGGCTCAA 
GTGGAAGAAA TGAGGTTGAT GATGAATCAG TTGGAAGAGG ACCTGGTTTC GGCGAGAAGG 
CGGAGTGACC TCTATGAATC GGAGCTCAGA GAGTCTCGGC TGGCTGCTGA AGAGTTTAAG 
CGGAAAGCAA CAGAATGTCA GCATAAACTG ATGAAGGCTA AAGATCAAGG GAAGCCTGAA 
GTGGGAGAAT ATGCCAAACT GGAGAAGATC AATGCTGAGC AGCAGCTCAA AATTCAGGAG 
CTCCAAGAGA AGCTAGAAAA GGCTGTGAAA GCTAGCACAG AGGCCACCGA GCTGCTGCAG 
AACATCCGTC AGGCGAAGGA GCGGGCCGAG CGTGAGCTGG AGAAGCTGCA CAACCGTGAG 
GACTCCTCCG AGGGCATCAA GAAGAAGCTG GTGGAGGCCG AGGAACGCCG CCACTCTCTG 
GAGAACAAGG TAAAGAGGCT AGAGACCATG GAGCGTAGAG AAAACAGACT GAAGGATGAC 
ATCCAGACAA AGTCCCAACA GATCCAGCAG ATGGCTGATA AAATTCTGGA GCTGGAGGAG 
AAGCACAGAG AAGCCCAGGT CTCAGCGCAG CATCTGGAGG TGCACCTGAA ACAGAAAGAG 
CAGCACTATG AAGAAAAAAT TAAAGTGTTG GACAATCAGA TAAAGAAAGA CCTAGCAGAC 
AAGGAGACAC TGGAGAACCT GATGCAAAGA CACGAAGAGG AGGCCCATGA GAAAGGCAAA 
ATTCTCAGCG AGCAGAAGGC GATGATCAAT GCTATGGATT CCAAGATCAG ATCCCTGGAA 
CAAAGGATAG TGGAATTGTC GGAAGCCAAT AAACTTGCGG CCAACAGCAG TCTCTTTACC 
CAGAGGAACA TGAAGGCCCA GGAAGAGATG ATTTCAGAAC TCAGGCAACA GAAGTTTTAC 
CTGGAGACGC AAGCTGGGAA ATTGGAGGCC CAGAACCGGA AGCTGGAGGA GCAGCTGGAA 
AAAATAAGCC ACCAAGACCA CAGTGACAAG AACCGTCTGC TGGAGCTGGA GACCAGGCTA 
AGGGAGGTCA GTCTAGAACA TGAAGAGCAG AAACTGGAGC TCAAACGCCA GCTCACAGAG 
CTGCAGCTCT CCCTGCAGGA GCGTGAATCC CAGCTGACAG CCCTGCAGGC AGCACGGGCG 
GCCCTGGAGA GCCAGCTCCG CCAGGCCAAG ACGGAGCTGG AAGAGACGAC AGCAGAAGCA 
GAAGAAGAGA TCCAGGCGCT TACGGCACAT AGAGATGAAA TCCAGCGCAA ATTCGATGCC 
CTTCGTAACA GCTGTACTGT GATCACGGAC TTGGAGGAGC AGCTAAACCA GCTGACGGAG 
GACAATGCCG AGCTCAACAA CCAAAACTTC TACTTGTCTA AACAACTCGA TGAGGCCTCT 
GGTGCCAACG ACGAGATTGT GCAGCTGCGA AGTGAAGTGG ACCATCTCCG CCGCGAGATC 
ACTGAGAGGG AGATGCAGCT CACTAGCCAG AAGCAAACGA TGGAGGCGCT GAAGACCACG 
TGCACAATGC TGGAGGAGCA GGTCATGGAC CTGGAGGCCC TGAACGACGA GCTGCTGGAG 
AAGGAGCGGC AGTGGGAGGC CTGGAGGAGT GTCCTTGGTG ACGAGAAGTC CCAGTTTGAG 
TGTCGGGTTC GAGAGTTGCA GAGGATGCTG GACACCGAAA AGCAGAGCAG GGCGAGGGCC 
GACCAGAGGA TCACTGAGTC GCGCCAGGTG GTCGAGCTGG CAGTAAAGGA GCACAAGGCC 
GAGATCCTGG CCTTGCAGCA GGCTCTCAAG GAGCAGAAGC TGAAGGCCGA GAGCCTCTCC 
GACAAGCTCA ATGATCTGGA GAAGAAGCAC GCCATGCTTG AAATGAATGC CCGAAGTTTA 
CAACAGAAGC TGGAGACAGA ACGAGAGCTC AAACAAAGGC TTCTGGAAGA GCAAGCCAAG 
TTACAGCAGC AGATGGACCT GCAGAAGAAT CATATTTTCC GTCTGACTCA AGGGCTGCAA 
GAAGCTCTGG ATCGGGCTGA TCTGCTGAAG ACAGAAAGGA GTGACCTGGA ATATCAGCTG 
GAAAACATTC AGGTTCTCTA TTCTCATGAG AAGGTGAAGA TGGAAGGCAC TATTTCCCAG 
CAAACCAAAC TCATCGATTT TCTGCAAGCC AAAATGGACC AGCCTGCCAA AAAGAAAAAG 
GGTTTATTTA GTCGACGGAA AGAGGACCCT GCTTTGCCCA CACAGGTTCC TCTGCAGTAC 
AATGAGCTGA AGGTGGCTCT GGAGAAGGAG AAAGCTCGCT GCGCGGAGCT CGAGGAAGCC 
CTGCAGAAGA CCCGCATCGA GCTCCGCTCC GCCCGGGAGG AAGCTGCCCA CCGGAAAGCT 
GCAGACCACC CACACCCATC TACGCCGGCT ACCGCCAGGC AGCAGATCGC CATGTCCGCC 
ATTGTGCGGT CACCCGAGCA CCAGCCCAGC GCCCTGAGCC TGCTCGCCCC GCCGTCCAGC 
CGCAGGAAGG AGTCTTCCAC TCCAGAGGAA TTCAGCCGGC GTCTTAAGGA GCGCATGCAC 
CACAACATCC CTCACCGGTT CAACGTGGGA CTGAACATGC GAGCCACGAA GTGCGCCGTG 
TGTCTGGATA CCGTGCACTT CGGGCGCCAG GCATCCAAGT GTCTCGAATG TCAGGTCATG 
TGCCACCCCA AGTGCTCCAC ATGCTTGCCA GCCACCTGTG GCCTGCCAGC CGAATACGCC 
ACACACTTCA CCGAGGCCTT CTGCCGCGAC AAAATGAACT CCCCGGGTCT GCAGTCCAAG 
GAGCCCAGCA GCAGCCTGCA CCTGGAAGGG TGGATGAAGG TGCCCAGGAA TAACAAGCGA 
GGACAGCAAG GCTGGGACAG GAAGTACATC GTTCTGGAGG GCTCCAAAGT CCTCATTTAT 
GACAACGAAG CCAGAGAAGC TGGACAGAGG CCGGTGGAAG AATTTGAGCT GTGCCTTCCC 
GACGGGGACG TGTCTATTCA TGGCGCCGTT GGTGCTTCTG AACTTGCAAA CACAGCCAAA 
GCAGATGTCC CCTATGTCCT GAAGATGGAG TCTCACCCCC ATACCACCTG CTGGCCTGGG 
AGGACCCTCT ACTTGCTGGC TCCCAGCTTT CCCGACAAGC AGCGCTGGGT CACCGCCTTA 
GAGTCAGTTG TGGCAGGTGG GAGAGTGTCT AGGGAAAAGG CGGAAGCCGA CGCTGGCCGT 
GACTGTACTT CCTACGAGCC TCTGCCCGCG TGGGCTCAGA AATTGCTCGG AAACTCCCTG 
TTGAAACTGG AAGGTGATGA CCGTCTGGAC ATGAACTGCA CGCTGCCCTT CAGCGACCAG 
GTGGTGCTGG TGGGCACCGA GGAAGGGCTG TATGCGCTGA ATGTCTTGAA GAACTCCCTC 
ACCCACGTCC CAGGAATCGG AGCAGTCTTC CAGATTTACA TCATCAAGGA CCTGGAGAAG 
CTCCTCATGA TCGCAGGAGA GGAGCGGGCC CTGTGTCTCG TGGATGTGAA GAAGGTGAAG 
CAGTCCCTCG CGCAGTCTCA CCTCCCCGCC CAGCCAGACA TCTCGCCCAA CATCTTTGAA 
GCGGTCAAGG GCTGCCACTT GTTTGCTGCT GGCAAGATTG AGAGCGGGCT CTGCATCTGT 
GCAGCCATGC CCAGCAAAGT TGTCATTCTC CGCTACAATG AAAACCTCAG CAAGTACTGC 
ATTCGCAAAG AGATCGAGAC CTCGGAGCCC TGCAGCTGTA TCCACTTCAC CAACTACAGT 
ATCCTCATCG GAACCAATAA ATTCTACGAA ATCGACATGA AGCAGTACAC GCTAGAGGAA 
TTCCTGGATA AGAACGACCA TTCCTTGGCG CCTGCCGTGT TTGCCTCCTC TTCCAACAGC 
TTCCCCGTGT CCATCATGCA GGTGAACGGC GCGGGGCAGC GGGAGGAGTT CCTGCTCTGT 
TTCCACGAAT TCGGGGTGTT CGTGGATTCT TATGGAAGAC GCAGCCGCAC AGATGATCTC 
AAGTGGAGTC GCTTACCTCT GGCCTTTGCC TACAGAGAAC CCTATCTGTT TGTGACTCAC 
TTCAACTCAC TCGAAGTAAT TGAGATCCAG GCCCGCTCCT CTCTGGGGAC CCCCGCCCGA 
GCATATTTGG AAATCCCCAA CCCACGCTAC CTGGGCCCTG CAATCTCCTC AGGAGCCATC 
TACCTGGCGT CCTCATATCA GGATAAGTTA AGGGTCATCT GCTGCAAAGG AAACCTGGTG 
AAGGAGACCG GCACCGACCA CCACCGTGGC CCATCCACCT CCCGCAGCCC CAACAAGCGA 
GGCCCGCCGA CGTACAACGA GCACATCACC AAGCGCGTGG CCTCAAGCCC GGCGCCTCCC 
GAAGGCCCCA GCCACCCTCG AGAGCCAAGC ACACCCCACC GCTACCGCGA GGGGCGGACC 
GAGCTGCGCA GGGACAAGTC TCCGGGCCGC CCCCTGGAGC GCGAGAAGTC CCCCGGCCGG 
ATGCTGAGCA CGCGGAGGGA GCGCTCCCCC GGGCGGCTGT TTGAGGACAG CAGCAGGGGC 
CGGCTGCCCG TGGGGGCCGT GAGGACCCCG CTGTCCCAGG TCAACAAGGT CTGGGACCAG 
TCTTCAGTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024833501.1
CDS	113..6364
Translation	MLKFKYGARNLLDAGTVESIASRASRLNLFFQGKPPSMTQQQMSLLSREAILDALFVLFKECSQPALMKIKHVSNFVQKYSDIIAELQELQPSVKDFEVKSVVGCGHFAEVQVVREKATGDVYAMKVMKKKALLAQEQVSYFEEEQNILSQSTSPWIPQLQYAFQDKNNLYLVMEYQPGGDLLSLLNRYEDQLDENMIQFYLAELILAVHSVHQMGYVHRDIKPENILIDRTGHIKLVDFGSAAKMNSNKMVNAKLPVGTPDYMAPEVLTVMNGDGKGAYSLDCDWWSVGVIAYEMVYGRCPFTEGTSAKTFNNIMNFQRFLKFPDDPKVSSELLDLIQSLLCGQKERLKFEGLCCHPFFSKIDWINIRNSPPPFVPTLKSDDDTSNFDEPEKNSWVSSSPCQLNPSGFSGEELPFVGFSYSKALGILGRSEPVVSSLDSPAKTSSMEKKLLIKSKELQDSQDKCHKMEQEMTRLHRRVSEVEAVLSQKEVELKASETQRSLLEQDLATYITECSSLKRSLEQARMEVSQEDDKALQLLHDIREQSRKLQEIKEQEYQAQVEEMRLMMNQLEEDLVSARRRSDLYESELRESRLAAEEFKRKATECQHKLMKAKDQGKPEVGEYAKLEKINAEQQLKIQELQEKLEKAVKASTEATELLQNIRQAKERAERELEKLHNREDSSEGIKKKLVEAEERRHSLENKVKRLETMERRENRLKDDIQTKSQQIQQMADKILELEEKHREAQVSAQHLEVHLKQKEQHYEEKIKVLDNQIKKDLADKETLENLMQRHEEEAHEKGKILSEQKAMINAMDSKIRSLEQRIVELSEANKLAANSSLFTQRNMKAQEEMISELRQQKFYLETQAGKLEAQNRKLEEQLEKISHQDHSDKNRLLELETRLREVSLEHEEQKLELKRQLTELQLSLQERESQLTALQAARAALESQLRQAKTELEETTAEAEEEIQALTAHRDEIQRKFDALRNSCTVITDLEEQLNQLTEDNAELNNQNFYLSKQLDEASGANDEIVQLRSEVDHLRREITEREMQLTSQKQTMEALKTTCTMLEEQVMDLEALNDELLEKERQWEAWRSVLGDEKSQFECRVRELQRMLDTEKQSRARADQRITESRQVVELAVKEHKAEILALQQALKEQKLKAESLSDKLNDLEKKHAMLEMNARSLQQKLETERELKQRLLEEQAKLQQQMDLQKNHIFRLTQGLQEALDRADLLKTERSDLEYQLENIQVLYSHEKVKMEGTISQQTKLIDFLQAKMDQPAKKKKGLFSRRKEDPALPTQVPLQYNELKVALEKEKARCAELEEALQKTRIELRSAREEAAHRKAADHPHPSTPATARQQIAMSAIVRSPEHQPSALSLLAPPSSRRKESSTPEEFSRRLKERMHHNIPHRFNVGLNMRATKCAVCLDTVHFGRQASKCLECQVMCHPKCSTCLPATCGLPAEYATHFTEAFCRDKMNSPGLQSKEPSSSLHLEGWMKVPRNNKRGQQGWDRKYIVLEGSKVLIYDNEAREAGQRPVEEFELCLPDGDVSIHGAVGASELANTAKADVPYVLKMESHPHTTCWPGRTLYLLAPSFPDKQRWVTALESVVAGGRVSREKAEADAGRDCTSYEPLPAWAQKLLGNSLLKLEGDDRLDMNCTLPFSDQVVLVGTEEGLYALNVLKNSLTHVPGIGAVFQIYIIKDLEKLLMIAGEERALCLVDVKKVKQSLAQSHLPAQPDISPNIFEAVKGCHLFAAGKIESGLCICAAMPSKVVILRYNENLSKYCIRKEIETSEPCSCIHFTNYSILIGTNKFYEIDMKQYTLEEFLDKNDHSLAPAVFASSSNSFPVSIMQVNGAGQREEFLLCFHEFGVFVDSYGRRSRTDDLKWSRLPLAFAYREPYLFVTHFNSLEVIEIQARSSLGTPARAYLEIPNPRYLGPAISSGAIYLASSYQDKLRVICCKGNLVKETGTDHHRGPSTSRSPNKRGPPTYNEHITKRVASSPAPPEGPSHPREPSTPHRYREGRTELRRDKSPGRPLEREKSPGRMLSTRRERSPGRLFEDSSRGRLPVGAVRTPLSQVNKVWDQSSV

Target ORF information:

RefSeq Version	XM_024977733.1
Organism	Bos taurus(cattle)
Definition	Bos taurus citron rho-interacting serine/threonine kinase (CIT), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024977733.1

ORF Insert Sequence:

ATGTTGAAGT TCAAGTATGG AGCACGGAAT CTACTGGACG CTGGTACTGT TGAATCCATT 
GCCAGCCGGG CCTCCAGGCT GAATCTTTTC TTCCAGGGGA AACCACCCTC CATGACTCAA 
CAGCAGATGT CTCTTCTTTC CCGAGAAGCG ATATTAGATG CCCTCTTTGT TCTCTTTAAA 
GAATGCAGTC AGCCAGCTCT GATGAAGATT AAGCACGTGA GCAACTTTGT CCAAAAGTAT 
TCCGATATCA TAGCTGAGTT GCAAGAGCTC CAGCCTTCAG TGAAGGACTT TGAAGTGAAA 
AGTGTCGTAG GCTGCGGTCA CTTTGCTGAA GTACAGGTGG TCAGAGAGAA AGCGACTGGG 
GATGTCTATG CCATGAAAGT CATGAAGAAG AAGGCCTTGT TGGCCCAGGA GCAGGTCTCA 
TATTTTGAGG AAGAACAGAA CATATTATCT CAGAGTACAA GTCCTTGGAT CCCCCAGTTG 
CAGTATGCCT TTCAGGACAA AAATAACCTT TATCTGGTCA TGGAATATCA GCCTGGAGGG 
GATTTGCTGT CACTTTTGAA TAGATATGAG GACCAATTAG ATGAAAATAT GATTCAGTTT 
TACCTAGCTG AACTGATTTT GGCTGTCCAC AGCGTTCATC AGATGGGCTA TGTACATCGA 
GACATCAAGC CCGAGAACAT CCTCATTGAC CGAACGGGAC ACATCAAGCT GGTGGATTTT 
GGATCAGCTG CTAAAATGAA CTCAAATAAG ATGGTAAATG CCAAACTCCC GGTTGGTACT 
CCAGATTACA TGGCCCCTGA AGTGTTGACT GTGATGAATG GGGATGGAAA AGGTGCCTAT 
AGCCTAGACT GTGATTGGTG GTCAGTGGGA GTTATTGCTT ATGAGATGGT TTATGGAAGA 
TGCCCATTCA CTGAAGGAAC CTCAGCCAAA ACCTTCAATA ACATCATGAA TTTCCAGCGG 
TTCTTGAAGT TTCCAGATGA CCCCAAAGTT AGCAGTGAAT TGCTTGATCT AATCCAGAGT 
TTGTTGTGTG GCCAGAAAGA GAGACTGAAG TTTGAGGGCC TTTGCTGTCA TCCTTTCTTC 
TCCAAAATTG ACTGGATTAA CATTCGTAAC TCTCCTCCCC CCTTCGTTCC CACCCTCAAG 
TCTGATGATG ACACCTCCAA TTTTGATGAA CCAGAGAAGA ATTCGTGGGT TTCATCCTCT 
CCGTGCCAGC TGAACCCCTC AGGTTTCTCG GGCGAAGAAC TGCCGTTTGT GGGGTTTTCG 
TATAGCAAGG CACTGGGAAT TCTTGGTAGA TCTGAGCCTG TTGTATCAAG TCTGGACTCC 
CCTGCCAAGA CTAGCTCCAT GGAAAAGAAA CTCCTTATCA AAAGCAAAGA GCTGCAGGAC 
TCCCAGGACA AGTGTCACAA GATGGAGCAG GAAATGACCC GGTTACATCG GAGAGTGTCA 
GAGGTGGAGG CTGTGCTTAG TCAGAAGGAG GTGGAGCTGA AGGCCTCTGA GACTCAGAGA 
TCCCTCCTGG AGCAGGACCT TGCTACCTAC ATCACAGAAT GCAGTAGCTT AAAGCGAAGT 
TTGGAGCAAG CACGGATGGA GGTGTCCCAA GAGGACGACA AAGCACTGCA GCTCCTCCAT 
GATATCAGAG AGCAGAGCCG GAAGCTCCAA GAAATCAAAG AGCAGGAGTA CCAGGCTCAA 
GTGGAAGAAA TGAGGTTGAT GATGAATCAG TTGGAAGAGG ACCTGGTTTC GGCGAGAAGG 
CGGAGTGACC TCTATGAATC GGAGCTCAGA GAGTCTCGGC TGGCTGCTGA AGAGTTTAAG 
CGGAAAGCAA CAGAATGTCA GCATAAACTG ATGAAGGCTA AAGATCAAGG GAAGCCTGAA 
GTGGGAGAAT ATGCCAAACT GGAGAAGATC AATGCTGAGC AGCAGCTCAA AATTCAGGAG 
CTCCAAGAGA AGCTAGAAAA GGCTGTGAAA GCTAGCACAG AGGCCACCGA GCTGCTGCAG 
AACATCCGTC AGGCGAAGGA GCGGGCCGAG CGTGAGCTGG AGAAGCTGCA CAACCGTGAG 
GACTCCTCCG AGGGCATCAA GAAGAAGCTG GTGGAGGCCG AGGAACGCCG CCACTCTCTG 
GAGAACAAGG TAAAGAGGCT AGAGACCATG GAGCGTAGAG AAAACAGACT GAAGGATGAC 
ATCCAGACAA AGTCCCAACA GATCCAGCAG ATGGCTGATA AAATTCTGGA GCTGGAGGAG 
AAGCACAGAG AAGCCCAGGT CTCAGCGCAG CATCTGGAGG TGCACCTGAA ACAGAAAGAG 
CAGCACTATG AAGAAAAAAT TAAAGTGTTG GACAATCAGA TAAAGAAAGA CCTAGCAGAC 
AAGGAGACAC TGGAGAACCT GATGCAAAGA CACGAAGAGG AGGCCCATGA GAAAGGCAAA 
ATTCTCAGCG AGCAGAAGGC GATGATCAAT GCTATGGATT CCAAGATCAG ATCCCTGGAA 
CAAAGGATAG TGGAATTGTC GGAAGCCAAT AAACTTGCGG CCAACAGCAG TCTCTTTACC 
CAGAGGAACA TGAAGGCCCA GGAAGAGATG ATTTCAGAAC TCAGGCAACA GAAGTTTTAC 
CTGGAGACGC AAGCTGGGAA ATTGGAGGCC CAGAACCGGA AGCTGGAGGA GCAGCTGGAA 
AAAATAAGCC ACCAAGACCA CAGTGACAAG AACCGTCTGC TGGAGCTGGA GACCAGGCTA 
AGGGAGGTCA GTCTAGAACA TGAAGAGCAG AAACTGGAGC TCAAACGCCA GCTCACAGAG 
CTGCAGCTCT CCCTGCAGGA GCGTGAATCC CAGCTGACAG CCCTGCAGGC AGCACGGGCG 
GCCCTGGAGA GCCAGCTCCG CCAGGCCAAG ACGGAGCTGG AAGAGACGAC AGCAGAAGCA 
GAAGAAGAGA TCCAGGCGCT TACGGCACAT AGAGATGAAA TCCAGCGCAA ATTCGATGCC 
CTTCGTAACA GCTGTACTGT GATCACGGAC TTGGAGGAGC AGCTAAACCA GCTGACGGAG 
GACAATGCCG AGCTCAACAA CCAAAACTTC TACTTGTCTA AACAACTCGA TGAGGCCTCT 
GGTGCCAACG ACGAGATTGT GCAGCTGCGA AGTGAAGTGG ACCATCTCCG CCGCGAGATC 
ACTGAGAGGG AGATGCAGCT CACTAGCCAG AAGCAAACGA TGGAGGCGCT GAAGACCACG 
TGCACAATGC TGGAGGAGCA GGTCATGGAC CTGGAGGCCC TGAACGACGA GCTGCTGGAG 
AAGGAGCGGC AGTGGGAGGC CTGGAGGAGT GTCCTTGGTG ACGAGAAGTC CCAGTTTGAG 
TGTCGGGTTC GAGAGTTGCA GAGGATGCTG GACACCGAAA AGCAGAGCAG GGCGAGGGCC 
GACCAGAGGA TCACTGAGTC GCGCCAGGTG GTCGAGCTGG CAGTAAAGGA GCACAAGGCC 
GAGATCCTGG CCTTGCAGCA GGCTCTCAAG GAGCAGAAGC TGAAGGCCGA GAGCCTCTCC 
GACAAGCTCA ATGATCTGGA GAAGAAGCAC GCCATGCTTG AAATGAATGC CCGAAGTTTA 
CAACAGAAGC TGGAGACAGA ACGAGAGCTC AAACAAAGGC TTCTGGAAGA GCAAGCCAAG 
TTACAGCAGC AGATGGACCT GCAGAAGAAT CATATTTTCC GTCTGACTCA AGGGCTGCAA 
GAAGCTCTGG ATCGGGCTGA TCTGCTGAAG ACAGAAAGGA GTGACCTGGA ATATCAGCTG 
GAAAACATTC AGGTTCTCTA TTCTCATGAG AAGGTGAAGA TGGAAGGCAC TATTTCCCAG 
CAAACCAAAC TCATCGATTT TCTGCAAGCC AAAATGGACC AGCCTGCCAA AAAGAAAAAG 
GGTTTATTTA GTCGACGGAA AGAGGACCCT GCTTTGCCCA CACAGGTTCC TCTGCAGTAC 
AATGAGCTGA AGGTGGCTCT GGAGAAGGAG AAAGCTCGCT GCGCGGAGCT CGAGGAAGCC 
CTGCAGAAGA CCCGCATCGA GCTCCGCTCC GCCCGGGAGG AAGCTGCCCA CCGGAAAGCT 
GCAGACCACC CACACCCATC TACGCCGGCT ACCGCCAGGC AGCAGATCGC CATGTCCGCC 
ATTGTGCGGT CACCCGAGCA CCAGCCCAGC GCCCTGAGCC TGCTCGCCCC GCCGTCCAGC 
CGCAGGAAGG AGTCTTCCAC TCCAGAGGAA TTCAGCCGGC GTCTTAAGGA GCGCATGCAC 
CACAACATCC CTCACCGGTT CAACGTGGGA CTGAACATGC GAGCCACGAA GTGCGCCGTG 
TGTCTGGATA CCGTGCACTT CGGGCGCCAG GCATCCAAGT GTCTCGAATG TCAGGTCATG 
TGCCACCCCA AGTGCTCCAC ATGCTTGCCA GCCACCTGTG GCCTGCCAGC CGAATACGCC 
ACACACTTCA CCGAGGCCTT CTGCCGCGAC AAAATGAACT CCCCGGGTCT GCAGTCCAAG 
GAGCCCAGCA GCAGCCTGCA CCTGGAAGGG TGGATGAAGG TGCCCAGGAA TAACAAGCGA 
GGACAGCAAG GCTGGGACAG GAAGTACATC GTTCTGGAGG GCTCCAAAGT CCTCATTTAT 
GACAACGAAG CCAGAGAAGC TGGACAGAGG CCGGTGGAAG AATTTGAGCT GTGCCTTCCC 
GACGGGGACG TGTCTATTCA TGGCGCCGTT GGTGCTTCTG AACTTGCAAA CACAGCCAAA 
GCAGATGTCC CCTATGTCCT GAAGATGGAG TCTCACCCCC ATACCACCTG CTGGCCTGGG 
AGGACCCTCT ACTTGCTGGC TCCCAGCTTT CCCGACAAGC AGCGCTGGGT CACCGCCTTA 
GAGTCAGTTG TGGCAGGTGG GAGAGTGTCT AGGGAAAAGG CGGAAGCCGA CGCTGGCCGT 
GACTGTACTT CCTACGAGCC TCTGCCCGCG TGGGCTCAGA AATTGCTCGG AAACTCCCTG 
TTGAAACTGG AAGGTGATGA CCGTCTGGAC ATGAACTGCA CGCTGCCCTT CAGCGACCAG 
GTGGTGCTGG TGGGCACCGA GGAAGGGCTG TATGCGCTGA ATGTCTTGAA GAACTCCCTC 
ACCCACGTCC CAGGAATCGG AGCAGTCTTC CAGATTTACA TCATCAAGGA CCTGGAGAAG 
CTCCTCATGA TCGCAGGAGA GGAGCGGGCC CTGTGTCTCG TGGATGTGAA GAAGGTGAAG 
CAGTCCCTCG CGCAGTCTCA CCTCCCCGCC CAGCCAGACA TCTCGCCCAA CATCTTTGAA 
GCGGTCAAGG GCTGCCACTT GTTTGCTGCT GGCAAGATTG AGAGCGGGCT CTGCATCTGT 
GCAGCCATGC CCAGCAAAGT TGTCATTCTC CGCTACAATG AAAACCTCAG CAAGTACTGC 
ATTCGCAAAG AGATCGAGAC CTCGGAGCCC TGCAGCTGTA TCCACTTCAC CAACTACAGT 
ATCCTCATCG GAACCAATAA ATTCTACGAA ATCGACATGA AGCAGTACAC GCTAGAGGAA 
TTCCTGGATA AGAACGACCA TTCCTTGGCG CCTGCCGTGT TTGCCTCCTC TTCCAACAGC 
TTCCCCGTGT CCATCATGCA GGTGAACGGC GCGGGGCAGC GGGAGGAGTT CCTGCTCTGT 
TTCCACGAAT TCGGGGTGTT CGTGGATTCT TATGGAAGAC GCAGCCGCAC AGATGATCTC 
AAGTGGAGTC GCTTACCTCT GGCCTTTGCC TACAGAGAAC CCTATCTGTT TGTGACTCAC 
TTCAACTCAC TCGAAGTAAT TGAGATCCAG GCCCGCTCCT CTCTGGGGAC CCCCGCCCGA 
GCATATTTGG AAATCCCCAA CCCACGCTAC CTGGGCCCTG CAATCTCCTC AGGAGCCATC 
TACCTGGCGT CCTCATATCA GGATAAGTTA AGGGTCATCT GCTGCAAAGG AAACCTGGTG 
AAGGAGACCG GCACCGACCA CCACCGTGGC CCATCCACCT CCCGCAGCCC CAACAAGCGA 
GGCCCGCCGA CGTACAACGA GCACATCACC AAGCGCGTGG CCTCAAGCCC GGCGCCTCCC 
GAAGGCCCCA GCCACCCTCG AGAGCCAAGC ACACCCCACC GCTACCGCGA GGGGCGGACC 
GAGCTGCGCA GGGACAAGTC TCCGGGCCGC CCCCTGGAGC GCGAGAAGTC CCCCGGCCGG 
ATGCTGAGCA CGCGGAGGGA GCGCTCCCCC GGGCGGCTGT TTGAGGACAG CAGCAGGGGC 
CGGCTGCCCG TGGGGGCCGT GAGGACCCCG CTGTCCCAGG TCAACAAGGT CTGGGACCAG 
TCTTCAGTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CIT ( XM_024977734.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024833502.1 Bos taurus citron rho-interacting serine/threonine kinase (CIT), transcript variant X2, mRNA.

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CloneID	OBa224622
Clone ID Related Accession (Same CDS sequence)	XM_024977733.1 , XM_024977734.1
Accession Version	XM_024977734.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6252bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	citron Rho-interacting kinase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGAAGT TCAAGTATGG AGCACGGAAT CTACTGGACG CTGGTACTGT TGAATCCATT 
GCCAGCCGGG CCTCCAGGCT GAATCTTTTC TTCCAGGGGA AACCACCCTC CATGACTCAA 
CAGCAGATGT CTCTTCTTTC CCGAGAAGCG ATATTAGATG CCCTCTTTGT TCTCTTTAAA 
GAATGCAGTC AGCCAGCTCT GATGAAGATT AAGCACGTGA GCAACTTTGT CCAAAAGTAT 
TCCGATATCA TAGCTGAGTT GCAAGAGCTC CAGCCTTCAG TGAAGGACTT TGAAGTGAAA 
AGTGTCGTAG GCTGCGGTCA CTTTGCTGAA GTACAGGTGG TCAGAGAGAA AGCGACTGGG 
GATGTCTATG CCATGAAAGT CATGAAGAAG AAGGCCTTGT TGGCCCAGGA GCAGGTCTCA 
TATTTTGAGG AAGAACAGAA CATATTATCT CAGAGTACAA GTCCTTGGAT CCCCCAGTTG 
CAGTATGCCT TTCAGGACAA AAATAACCTT TATCTGGTCA TGGAATATCA GCCTGGAGGG 
GATTTGCTGT CACTTTTGAA TAGATATGAG GACCAATTAG ATGAAAATAT GATTCAGTTT 
TACCTAGCTG AACTGATTTT GGCTGTCCAC AGCGTTCATC AGATGGGCTA TGTACATCGA 
GACATCAAGC CCGAGAACAT CCTCATTGAC CGAACGGGAC ACATCAAGCT GGTGGATTTT 
GGATCAGCTG CTAAAATGAA CTCAAATAAG ATGGTAAATG CCAAACTCCC GGTTGGTACT 
CCAGATTACA TGGCCCCTGA AGTGTTGACT GTGATGAATG GGGATGGAAA AGGTGCCTAT 
AGCCTAGACT GTGATTGGTG GTCAGTGGGA GTTATTGCTT ATGAGATGGT TTATGGAAGA 
TGCCCATTCA CTGAAGGAAC CTCAGCCAAA ACCTTCAATA ACATCATGAA TTTCCAGCGG 
TTCTTGAAGT TTCCAGATGA CCCCAAAGTT AGCAGTGAAT TGCTTGATCT AATCCAGAGT 
TTGTTGTGTG GCCAGAAAGA GAGACTGAAG TTTGAGGGCC TTTGCTGTCA TCCTTTCTTC 
TCCAAAATTG ACTGGATTAA CATTCGTAAC TCTCCTCCCC CCTTCGTTCC CACCCTCAAG 
TCTGATGATG ACACCTCCAA TTTTGATGAA CCAGAGAAGA ATTCGTGGGT TTCATCCTCT 
CCGTGCCAGC TGAACCCCTC AGGTTTCTCG GGCGAAGAAC TGCCGTTTGT GGGGTTTTCG 
TATAGCAAGG CACTGGGAAT TCTTGGTAGA TCTGAGCCTG TTGTATCAAG TCTGGACTCC 
CCTGCCAAGA CTAGCTCCAT GGAAAAGAAA CTCCTTATCA AAAGCAAAGA GCTGCAGGAC 
TCCCAGGACA AGTGTCACAA GATGGAGCAG GAAATGACCC GGTTACATCG GAGAGTGTCA 
GAGGTGGAGG CTGTGCTTAG TCAGAAGGAG GTGGAGCTGA AGGCCTCTGA GACTCAGAGA 
TCCCTCCTGG AGCAGGACCT TGCTACCTAC ATCACAGAAT GCAGTAGCTT AAAGCGAAGT 
TTGGAGCAAG CACGGATGGA GGTGTCCCAA GAGGACGACA AAGCACTGCA GCTCCTCCAT 
GATATCAGAG AGCAGAGCCG GAAGCTCCAA GAAATCAAAG AGCAGGAGTA CCAGGCTCAA 
GTGGAAGAAA TGAGGTTGAT GATGAATCAG TTGGAAGAGG ACCTGGTTTC GGCGAGAAGG 
CGGAGTGACC TCTATGAATC GGAGCTCAGA GAGTCTCGGC TGGCTGCTGA AGAGTTTAAG 
CGGAAAGCAA CAGAATGTCA GCATAAACTG ATGAAGGCTA AAGATCAAGG GAAGCCTGAA 
GTGGGAGAAT ATGCCAAACT GGAGAAGATC AATGCTGAGC AGCAGCTCAA AATTCAGGAG 
CTCCAAGAGA AGCTAGAAAA GGCTGTGAAA GCTAGCACAG AGGCCACCGA GCTGCTGCAG 
AACATCCGTC AGGCGAAGGA GCGGGCCGAG CGTGAGCTGG AGAAGCTGCA CAACCGTGAG 
GACTCCTCCG AGGGCATCAA GAAGAAGCTG GTGGAGGCCG AGGAACGCCG CCACTCTCTG 
GAGAACAAGG TAAAGAGGCT AGAGACCATG GAGCGTAGAG AAAACAGACT GAAGGATGAC 
ATCCAGACAA AGTCCCAACA GATCCAGCAG ATGGCTGATA AAATTCTGGA GCTGGAGGAG 
AAGCACAGAG AAGCCCAGGT CTCAGCGCAG CATCTGGAGG TGCACCTGAA ACAGAAAGAG 
CAGCACTATG AAGAAAAAAT TAAAGTGTTG GACAATCAGA TAAAGAAAGA CCTAGCAGAC 
AAGGAGACAC TGGAGAACCT GATGCAAAGA CACGAAGAGG AGGCCCATGA GAAAGGCAAA 
ATTCTCAGCG AGCAGAAGGC GATGATCAAT GCTATGGATT CCAAGATCAG ATCCCTGGAA 
CAAAGGATAG TGGAATTGTC GGAAGCCAAT AAACTTGCGG CCAACAGCAG TCTCTTTACC 
CAGAGGAACA TGAAGGCCCA GGAAGAGATG ATTTCAGAAC TCAGGCAACA GAAGTTTTAC 
CTGGAGACGC AAGCTGGGAA ATTGGAGGCC CAGAACCGGA AGCTGGAGGA GCAGCTGGAA 
AAAATAAGCC ACCAAGACCA CAGTGACAAG AACCGTCTGC TGGAGCTGGA GACCAGGCTA 
AGGGAGGTCA GTCTAGAACA TGAAGAGCAG AAACTGGAGC TCAAACGCCA GCTCACAGAG 
CTGCAGCTCT CCCTGCAGGA GCGTGAATCC CAGCTGACAG CCCTGCAGGC AGCACGGGCG 
GCCCTGGAGA GCCAGCTCCG CCAGGCCAAG ACGGAGCTGG AAGAGACGAC AGCAGAAGCA 
GAAGAAGAGA TCCAGGCGCT TACGGCACAT AGAGATGAAA TCCAGCGCAA ATTCGATGCC 
CTTCGTAACA GCTGTACTGT GATCACGGAC TTGGAGGAGC AGCTAAACCA GCTGACGGAG 
GACAATGCCG AGCTCAACAA CCAAAACTTC TACTTGTCTA AACAACTCGA TGAGGCCTCT 
GGTGCCAACG ACGAGATTGT GCAGCTGCGA AGTGAAGTGG ACCATCTCCG CCGCGAGATC 
ACTGAGAGGG AGATGCAGCT CACTAGCCAG AAGCAAACGA TGGAGGCGCT GAAGACCACG 
TGCACAATGC TGGAGGAGCA GGTCATGGAC CTGGAGGCCC TGAACGACGA GCTGCTGGAG 
AAGGAGCGGC AGTGGGAGGC CTGGAGGAGT GTCCTTGGTG ACGAGAAGTC CCAGTTTGAG 
TGTCGGGTTC GAGAGTTGCA GAGGATGCTG GACACCGAAA AGCAGAGCAG GGCGAGGGCC 
GACCAGAGGA TCACTGAGTC GCGCCAGGTG GTCGAGCTGG CAGTAAAGGA GCACAAGGCC 
GAGATCCTGG CCTTGCAGCA GGCTCTCAAG GAGCAGAAGC TGAAGGCCGA GAGCCTCTCC 
GACAAGCTCA ATGATCTGGA GAAGAAGCAC GCCATGCTTG AAATGAATGC CCGAAGTTTA 
CAACAGAAGC TGGAGACAGA ACGAGAGCTC AAACAAAGGC TTCTGGAAGA GCAAGCCAAG 
TTACAGCAGC AGATGGACCT GCAGAAGAAT CATATTTTCC GTCTGACTCA AGGGCTGCAA 
GAAGCTCTGG ATCGGGCTGA TCTGCTGAAG ACAGAAAGGA GTGACCTGGA ATATCAGCTG 
GAAAACATTC AGGTTCTCTA TTCTCATGAG AAGGTGAAGA TGGAAGGCAC TATTTCCCAG 
CAAACCAAAC TCATCGATTT TCTGCAAGCC AAAATGGACC AGCCTGCCAA AAAGAAAAAG 
GGTTTATTTA GTCGACGGAA AGAGGACCCT GCTTTGCCCA CACAGGTTCC TCTGCAGTAC 
AATGAGCTGA AGGTGGCTCT GGAGAAGGAG AAAGCTCGCT GCGCGGAGCT CGAGGAAGCC 
CTGCAGAAGA CCCGCATCGA GCTCCGCTCC GCCCGGGAGG AAGCTGCCCA CCGGAAAGCT 
GCAGACCACC CACACCCATC TACGCCGGCT ACCGCCAGGC AGCAGATCGC CATGTCCGCC 
ATTGTGCGGT CACCCGAGCA CCAGCCCAGC GCCCTGAGCC TGCTCGCCCC GCCGTCCAGC 
CGCAGGAAGG AGTCTTCCAC TCCAGAGGAA TTCAGCCGGC GTCTTAAGGA GCGCATGCAC 
CACAACATCC CTCACCGGTT CAACGTGGGA CTGAACATGC GAGCCACGAA GTGCGCCGTG 
TGTCTGGATA CCGTGCACTT CGGGCGCCAG GCATCCAAGT GTCTCGAATG TCAGGTCATG 
TGCCACCCCA AGTGCTCCAC ATGCTTGCCA GCCACCTGTG GCCTGCCAGC CGAATACGCC 
ACACACTTCA CCGAGGCCTT CTGCCGCGAC AAAATGAACT CCCCGGGTCT GCAGTCCAAG 
GAGCCCAGCA GCAGCCTGCA CCTGGAAGGG TGGATGAAGG TGCCCAGGAA TAACAAGCGA 
GGACAGCAAG GCTGGGACAG GAAGTACATC GTTCTGGAGG GCTCCAAAGT CCTCATTTAT 
GACAACGAAG CCAGAGAAGC TGGACAGAGG CCGGTGGAAG AATTTGAGCT GTGCCTTCCC 
GACGGGGACG TGTCTATTCA TGGCGCCGTT GGTGCTTCTG AACTTGCAAA CACAGCCAAA 
GCAGATGTCC CCTATGTCCT GAAGATGGAG TCTCACCCCC ATACCACCTG CTGGCCTGGG 
AGGACCCTCT ACTTGCTGGC TCCCAGCTTT CCCGACAAGC AGCGCTGGGT CACCGCCTTA 
GAGTCAGTTG TGGCAGGTGG GAGAGTGTCT AGGGAAAAGG CGGAAGCCGA CGCTGGCCGT 
GACTGTACTT CCTACGAGCC TCTGCCCGCG TGGGCTCAGA AATTGCTCGG AAACTCCCTG 
TTGAAACTGG AAGGTGATGA CCGTCTGGAC ATGAACTGCA CGCTGCCCTT CAGCGACCAG 
GTGGTGCTGG TGGGCACCGA GGAAGGGCTG TATGCGCTGA ATGTCTTGAA GAACTCCCTC 
ACCCACGTCC CAGGAATCGG AGCAGTCTTC CAGATTTACA TCATCAAGGA CCTGGAGAAG 
CTCCTCATGA TCGCAGGAGA GGAGCGGGCC CTGTGTCTCG TGGATGTGAA GAAGGTGAAG 
CAGTCCCTCG CGCAGTCTCA CCTCCCCGCC CAGCCAGACA TCTCGCCCAA CATCTTTGAA 
GCGGTCAAGG GCTGCCACTT GTTTGCTGCT GGCAAGATTG AGAGCGGGCT CTGCATCTGT 
GCAGCCATGC CCAGCAAAGT TGTCATTCTC CGCTACAATG AAAACCTCAG CAAGTACTGC 
ATTCGCAAAG AGATCGAGAC CTCGGAGCCC TGCAGCTGTA TCCACTTCAC CAACTACAGT 
ATCCTCATCG GAACCAATAA ATTCTACGAA ATCGACATGA AGCAGTACAC GCTAGAGGAA 
TTCCTGGATA AGAACGACCA TTCCTTGGCG CCTGCCGTGT TTGCCTCCTC TTCCAACAGC 
TTCCCCGTGT CCATCATGCA GGTGAACGGC GCGGGGCAGC GGGAGGAGTT CCTGCTCTGT 
TTCCACGAAT TCGGGGTGTT CGTGGATTCT TATGGAAGAC GCAGCCGCAC AGATGATCTC 
AAGTGGAGTC GCTTACCTCT GGCCTTTGCC TACAGAGAAC CCTATCTGTT TGTGACTCAC 
TTCAACTCAC TCGAAGTAAT TGAGATCCAG GCCCGCTCCT CTCTGGGGAC CCCCGCCCGA 
GCATATTTGG AAATCCCCAA CCCACGCTAC CTGGGCCCTG CAATCTCCTC AGGAGCCATC 
TACCTGGCGT CCTCATATCA GGATAAGTTA AGGGTCATCT GCTGCAAAGG AAACCTGGTG 
AAGGAGACCG GCACCGACCA CCACCGTGGC CCATCCACCT CCCGCAGCCC CAACAAGCGA 
GGCCCGCCGA CGTACAACGA GCACATCACC AAGCGCGTGG CCTCAAGCCC GGCGCCTCCC 
GAAGGCCCCA GCCACCCTCG AGAGCCAAGC ACACCCCACC GCTACCGCGA GGGGCGGACC 
GAGCTGCGCA GGGACAAGTC TCCGGGCCGC CCCCTGGAGC GCGAGAAGTC CCCCGGCCGG 
ATGCTGAGCA CGCGGAGGGA GCGCTCCCCC GGGCGGCTGT TTGAGGACAG CAGCAGGGGC 
CGGCTGCCCG TGGGGGCCGT GAGGACCCCG CTGTCCCAGG TCAACAAGGT CTGGGACCAG 
TCTTCAGTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024833502.1
CDS	52..6303
Translation	MLKFKYGARNLLDAGTVESIASRASRLNLFFQGKPPSMTQQQMSLLSREAILDALFVLFKECSQPALMKIKHVSNFVQKYSDIIAELQELQPSVKDFEVKSVVGCGHFAEVQVVREKATGDVYAMKVMKKKALLAQEQVSYFEEEQNILSQSTSPWIPQLQYAFQDKNNLYLVMEYQPGGDLLSLLNRYEDQLDENMIQFYLAELILAVHSVHQMGYVHRDIKPENILIDRTGHIKLVDFGSAAKMNSNKMVNAKLPVGTPDYMAPEVLTVMNGDGKGAYSLDCDWWSVGVIAYEMVYGRCPFTEGTSAKTFNNIMNFQRFLKFPDDPKVSSELLDLIQSLLCGQKERLKFEGLCCHPFFSKIDWINIRNSPPPFVPTLKSDDDTSNFDEPEKNSWVSSSPCQLNPSGFSGEELPFVGFSYSKALGILGRSEPVVSSLDSPAKTSSMEKKLLIKSKELQDSQDKCHKMEQEMTRLHRRVSEVEAVLSQKEVELKASETQRSLLEQDLATYITECSSLKRSLEQARMEVSQEDDKALQLLHDIREQSRKLQEIKEQEYQAQVEEMRLMMNQLEEDLVSARRRSDLYESELRESRLAAEEFKRKATECQHKLMKAKDQGKPEVGEYAKLEKINAEQQLKIQELQEKLEKAVKASTEATELLQNIRQAKERAERELEKLHNREDSSEGIKKKLVEAEERRHSLENKVKRLETMERRENRLKDDIQTKSQQIQQMADKILELEEKHREAQVSAQHLEVHLKQKEQHYEEKIKVLDNQIKKDLADKETLENLMQRHEEEAHEKGKILSEQKAMINAMDSKIRSLEQRIVELSEANKLAANSSLFTQRNMKAQEEMISELRQQKFYLETQAGKLEAQNRKLEEQLEKISHQDHSDKNRLLELETRLREVSLEHEEQKLELKRQLTELQLSLQERESQLTALQAARAALESQLRQAKTELEETTAEAEEEIQALTAHRDEIQRKFDALRNSCTVITDLEEQLNQLTEDNAELNNQNFYLSKQLDEASGANDEIVQLRSEVDHLRREITEREMQLTSQKQTMEALKTTCTMLEEQVMDLEALNDELLEKERQWEAWRSVLGDEKSQFECRVRELQRMLDTEKQSRARADQRITESRQVVELAVKEHKAEILALQQALKEQKLKAESLSDKLNDLEKKHAMLEMNARSLQQKLETERELKQRLLEEQAKLQQQMDLQKNHIFRLTQGLQEALDRADLLKTERSDLEYQLENIQVLYSHEKVKMEGTISQQTKLIDFLQAKMDQPAKKKKGLFSRRKEDPALPTQVPLQYNELKVALEKEKARCAELEEALQKTRIELRSAREEAAHRKAADHPHPSTPATARQQIAMSAIVRSPEHQPSALSLLAPPSSRRKESSTPEEFSRRLKERMHHNIPHRFNVGLNMRATKCAVCLDTVHFGRQASKCLECQVMCHPKCSTCLPATCGLPAEYATHFTEAFCRDKMNSPGLQSKEPSSSLHLEGWMKVPRNNKRGQQGWDRKYIVLEGSKVLIYDNEAREAGQRPVEEFELCLPDGDVSIHGAVGASELANTAKADVPYVLKMESHPHTTCWPGRTLYLLAPSFPDKQRWVTALESVVAGGRVSREKAEADAGRDCTSYEPLPAWAQKLLGNSLLKLEGDDRLDMNCTLPFSDQVVLVGTEEGLYALNVLKNSLTHVPGIGAVFQIYIIKDLEKLLMIAGEERALCLVDVKKVKQSLAQSHLPAQPDISPNIFEAVKGCHLFAAGKIESGLCICAAMPSKVVILRYNENLSKYCIRKEIETSEPCSCIHFTNYSILIGTNKFYEIDMKQYTLEEFLDKNDHSLAPAVFASSSNSFPVSIMQVNGAGQREEFLLCFHEFGVFVDSYGRRSRTDDLKWSRLPLAFAYREPYLFVTHFNSLEVIEIQARSSLGTPARAYLEIPNPRYLGPAISSGAIYLASSYQDKLRVICCKGNLVKETGTDHHRGPSTSRSPNKRGPPTYNEHITKRVASSPAPPEGPSHPREPSTPHRYREGRTELRRDKSPGRPLEREKSPGRMLSTRRERSPGRLFEDSSRGRLPVGAVRTPLSQVNKVWDQSSV

Target ORF information:

RefSeq Version	XM_024977734.1
Organism	Bos taurus(cattle)
Definition	Bos taurus citron rho-interacting serine/threonine kinase (CIT), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024977734.1

ORF Insert Sequence:

ATGTTGAAGT TCAAGTATGG AGCACGGAAT CTACTGGACG CTGGTACTGT TGAATCCATT 
GCCAGCCGGG CCTCCAGGCT GAATCTTTTC TTCCAGGGGA AACCACCCTC CATGACTCAA 
CAGCAGATGT CTCTTCTTTC CCGAGAAGCG ATATTAGATG CCCTCTTTGT TCTCTTTAAA 
GAATGCAGTC AGCCAGCTCT GATGAAGATT AAGCACGTGA GCAACTTTGT CCAAAAGTAT 
TCCGATATCA TAGCTGAGTT GCAAGAGCTC CAGCCTTCAG TGAAGGACTT TGAAGTGAAA 
AGTGTCGTAG GCTGCGGTCA CTTTGCTGAA GTACAGGTGG TCAGAGAGAA AGCGACTGGG 
GATGTCTATG CCATGAAAGT CATGAAGAAG AAGGCCTTGT TGGCCCAGGA GCAGGTCTCA 
TATTTTGAGG AAGAACAGAA CATATTATCT CAGAGTACAA GTCCTTGGAT CCCCCAGTTG 
CAGTATGCCT TTCAGGACAA AAATAACCTT TATCTGGTCA TGGAATATCA GCCTGGAGGG 
GATTTGCTGT CACTTTTGAA TAGATATGAG GACCAATTAG ATGAAAATAT GATTCAGTTT 
TACCTAGCTG AACTGATTTT GGCTGTCCAC AGCGTTCATC AGATGGGCTA TGTACATCGA 
GACATCAAGC CCGAGAACAT CCTCATTGAC CGAACGGGAC ACATCAAGCT GGTGGATTTT 
GGATCAGCTG CTAAAATGAA CTCAAATAAG ATGGTAAATG CCAAACTCCC GGTTGGTACT 
CCAGATTACA TGGCCCCTGA AGTGTTGACT GTGATGAATG GGGATGGAAA AGGTGCCTAT 
AGCCTAGACT GTGATTGGTG GTCAGTGGGA GTTATTGCTT ATGAGATGGT TTATGGAAGA 
TGCCCATTCA CTGAAGGAAC CTCAGCCAAA ACCTTCAATA ACATCATGAA TTTCCAGCGG 
TTCTTGAAGT TTCCAGATGA CCCCAAAGTT AGCAGTGAAT TGCTTGATCT AATCCAGAGT 
TTGTTGTGTG GCCAGAAAGA GAGACTGAAG TTTGAGGGCC TTTGCTGTCA TCCTTTCTTC 
TCCAAAATTG ACTGGATTAA CATTCGTAAC TCTCCTCCCC CCTTCGTTCC CACCCTCAAG 
TCTGATGATG ACACCTCCAA TTTTGATGAA CCAGAGAAGA ATTCGTGGGT TTCATCCTCT 
CCGTGCCAGC TGAACCCCTC AGGTTTCTCG GGCGAAGAAC TGCCGTTTGT GGGGTTTTCG 
TATAGCAAGG CACTGGGAAT TCTTGGTAGA TCTGAGCCTG TTGTATCAAG TCTGGACTCC 
CCTGCCAAGA CTAGCTCCAT GGAAAAGAAA CTCCTTATCA AAAGCAAAGA GCTGCAGGAC 
TCCCAGGACA AGTGTCACAA GATGGAGCAG GAAATGACCC GGTTACATCG GAGAGTGTCA 
GAGGTGGAGG CTGTGCTTAG TCAGAAGGAG GTGGAGCTGA AGGCCTCTGA GACTCAGAGA 
TCCCTCCTGG AGCAGGACCT TGCTACCTAC ATCACAGAAT GCAGTAGCTT AAAGCGAAGT 
TTGGAGCAAG CACGGATGGA GGTGTCCCAA GAGGACGACA AAGCACTGCA GCTCCTCCAT 
GATATCAGAG AGCAGAGCCG GAAGCTCCAA GAAATCAAAG AGCAGGAGTA CCAGGCTCAA 
GTGGAAGAAA TGAGGTTGAT GATGAATCAG TTGGAAGAGG ACCTGGTTTC GGCGAGAAGG 
CGGAGTGACC TCTATGAATC GGAGCTCAGA GAGTCTCGGC TGGCTGCTGA AGAGTTTAAG 
CGGAAAGCAA CAGAATGTCA GCATAAACTG ATGAAGGCTA AAGATCAAGG GAAGCCTGAA 
GTGGGAGAAT ATGCCAAACT GGAGAAGATC AATGCTGAGC AGCAGCTCAA AATTCAGGAG 
CTCCAAGAGA AGCTAGAAAA GGCTGTGAAA GCTAGCACAG AGGCCACCGA GCTGCTGCAG 
AACATCCGTC AGGCGAAGGA GCGGGCCGAG CGTGAGCTGG AGAAGCTGCA CAACCGTGAG 
GACTCCTCCG AGGGCATCAA GAAGAAGCTG GTGGAGGCCG AGGAACGCCG CCACTCTCTG 
GAGAACAAGG TAAAGAGGCT AGAGACCATG GAGCGTAGAG AAAACAGACT GAAGGATGAC 
ATCCAGACAA AGTCCCAACA GATCCAGCAG ATGGCTGATA AAATTCTGGA GCTGGAGGAG 
AAGCACAGAG AAGCCCAGGT CTCAGCGCAG CATCTGGAGG TGCACCTGAA ACAGAAAGAG 
CAGCACTATG AAGAAAAAAT TAAAGTGTTG GACAATCAGA TAAAGAAAGA CCTAGCAGAC 
AAGGAGACAC TGGAGAACCT GATGCAAAGA CACGAAGAGG AGGCCCATGA GAAAGGCAAA 
ATTCTCAGCG AGCAGAAGGC GATGATCAAT GCTATGGATT CCAAGATCAG ATCCCTGGAA 
CAAAGGATAG TGGAATTGTC GGAAGCCAAT AAACTTGCGG CCAACAGCAG TCTCTTTACC 
CAGAGGAACA TGAAGGCCCA GGAAGAGATG ATTTCAGAAC TCAGGCAACA GAAGTTTTAC 
CTGGAGACGC AAGCTGGGAA ATTGGAGGCC CAGAACCGGA AGCTGGAGGA GCAGCTGGAA 
AAAATAAGCC ACCAAGACCA CAGTGACAAG AACCGTCTGC TGGAGCTGGA GACCAGGCTA 
AGGGAGGTCA GTCTAGAACA TGAAGAGCAG AAACTGGAGC TCAAACGCCA GCTCACAGAG 
CTGCAGCTCT CCCTGCAGGA GCGTGAATCC CAGCTGACAG CCCTGCAGGC AGCACGGGCG 
GCCCTGGAGA GCCAGCTCCG CCAGGCCAAG ACGGAGCTGG AAGAGACGAC AGCAGAAGCA 
GAAGAAGAGA TCCAGGCGCT TACGGCACAT AGAGATGAAA TCCAGCGCAA ATTCGATGCC 
CTTCGTAACA GCTGTACTGT GATCACGGAC TTGGAGGAGC AGCTAAACCA GCTGACGGAG 
GACAATGCCG AGCTCAACAA CCAAAACTTC TACTTGTCTA AACAACTCGA TGAGGCCTCT 
GGTGCCAACG ACGAGATTGT GCAGCTGCGA AGTGAAGTGG ACCATCTCCG CCGCGAGATC 
ACTGAGAGGG AGATGCAGCT CACTAGCCAG AAGCAAACGA TGGAGGCGCT GAAGACCACG 
TGCACAATGC TGGAGGAGCA GGTCATGGAC CTGGAGGCCC TGAACGACGA GCTGCTGGAG 
AAGGAGCGGC AGTGGGAGGC CTGGAGGAGT GTCCTTGGTG ACGAGAAGTC CCAGTTTGAG 
TGTCGGGTTC GAGAGTTGCA GAGGATGCTG GACACCGAAA AGCAGAGCAG GGCGAGGGCC 
GACCAGAGGA TCACTGAGTC GCGCCAGGTG GTCGAGCTGG CAGTAAAGGA GCACAAGGCC 
GAGATCCTGG CCTTGCAGCA GGCTCTCAAG GAGCAGAAGC TGAAGGCCGA GAGCCTCTCC 
GACAAGCTCA ATGATCTGGA GAAGAAGCAC GCCATGCTTG AAATGAATGC CCGAAGTTTA 
CAACAGAAGC TGGAGACAGA ACGAGAGCTC AAACAAAGGC TTCTGGAAGA GCAAGCCAAG 
TTACAGCAGC AGATGGACCT GCAGAAGAAT CATATTTTCC GTCTGACTCA AGGGCTGCAA 
GAAGCTCTGG ATCGGGCTGA TCTGCTGAAG ACAGAAAGGA GTGACCTGGA ATATCAGCTG 
GAAAACATTC AGGTTCTCTA TTCTCATGAG AAGGTGAAGA TGGAAGGCAC TATTTCCCAG 
CAAACCAAAC TCATCGATTT TCTGCAAGCC AAAATGGACC AGCCTGCCAA AAAGAAAAAG 
GGTTTATTTA GTCGACGGAA AGAGGACCCT GCTTTGCCCA CACAGGTTCC TCTGCAGTAC 
AATGAGCTGA AGGTGGCTCT GGAGAAGGAG AAAGCTCGCT GCGCGGAGCT CGAGGAAGCC 
CTGCAGAAGA CCCGCATCGA GCTCCGCTCC GCCCGGGAGG AAGCTGCCCA CCGGAAAGCT 
GCAGACCACC CACACCCATC TACGCCGGCT ACCGCCAGGC AGCAGATCGC CATGTCCGCC 
ATTGTGCGGT CACCCGAGCA CCAGCCCAGC GCCCTGAGCC TGCTCGCCCC GCCGTCCAGC 
CGCAGGAAGG AGTCTTCCAC TCCAGAGGAA TTCAGCCGGC GTCTTAAGGA GCGCATGCAC 
CACAACATCC CTCACCGGTT CAACGTGGGA CTGAACATGC GAGCCACGAA GTGCGCCGTG 
TGTCTGGATA CCGTGCACTT CGGGCGCCAG GCATCCAAGT GTCTCGAATG TCAGGTCATG 
TGCCACCCCA AGTGCTCCAC ATGCTTGCCA GCCACCTGTG GCCTGCCAGC CGAATACGCC 
ACACACTTCA CCGAGGCCTT CTGCCGCGAC AAAATGAACT CCCCGGGTCT GCAGTCCAAG 
GAGCCCAGCA GCAGCCTGCA CCTGGAAGGG TGGATGAAGG TGCCCAGGAA TAACAAGCGA 
GGACAGCAAG GCTGGGACAG GAAGTACATC GTTCTGGAGG GCTCCAAAGT CCTCATTTAT 
GACAACGAAG CCAGAGAAGC TGGACAGAGG CCGGTGGAAG AATTTGAGCT GTGCCTTCCC 
GACGGGGACG TGTCTATTCA TGGCGCCGTT GGTGCTTCTG AACTTGCAAA CACAGCCAAA 
GCAGATGTCC CCTATGTCCT GAAGATGGAG TCTCACCCCC ATACCACCTG CTGGCCTGGG 
AGGACCCTCT ACTTGCTGGC TCCCAGCTTT CCCGACAAGC AGCGCTGGGT CACCGCCTTA 
GAGTCAGTTG TGGCAGGTGG GAGAGTGTCT AGGGAAAAGG CGGAAGCCGA CGCTGGCCGT 
GACTGTACTT CCTACGAGCC TCTGCCCGCG TGGGCTCAGA AATTGCTCGG AAACTCCCTG 
TTGAAACTGG AAGGTGATGA CCGTCTGGAC ATGAACTGCA CGCTGCCCTT CAGCGACCAG 
GTGGTGCTGG TGGGCACCGA GGAAGGGCTG TATGCGCTGA ATGTCTTGAA GAACTCCCTC 
ACCCACGTCC CAGGAATCGG AGCAGTCTTC CAGATTTACA TCATCAAGGA CCTGGAGAAG 
CTCCTCATGA TCGCAGGAGA GGAGCGGGCC CTGTGTCTCG TGGATGTGAA GAAGGTGAAG 
CAGTCCCTCG CGCAGTCTCA CCTCCCCGCC CAGCCAGACA TCTCGCCCAA CATCTTTGAA 
GCGGTCAAGG GCTGCCACTT GTTTGCTGCT GGCAAGATTG AGAGCGGGCT CTGCATCTGT 
GCAGCCATGC CCAGCAAAGT TGTCATTCTC CGCTACAATG AAAACCTCAG CAAGTACTGC 
ATTCGCAAAG AGATCGAGAC CTCGGAGCCC TGCAGCTGTA TCCACTTCAC CAACTACAGT 
ATCCTCATCG GAACCAATAA ATTCTACGAA ATCGACATGA AGCAGTACAC GCTAGAGGAA 
TTCCTGGATA AGAACGACCA TTCCTTGGCG CCTGCCGTGT TTGCCTCCTC TTCCAACAGC 
TTCCCCGTGT CCATCATGCA GGTGAACGGC GCGGGGCAGC GGGAGGAGTT CCTGCTCTGT 
TTCCACGAAT TCGGGGTGTT CGTGGATTCT TATGGAAGAC GCAGCCGCAC AGATGATCTC 
AAGTGGAGTC GCTTACCTCT GGCCTTTGCC TACAGAGAAC CCTATCTGTT TGTGACTCAC 
TTCAACTCAC TCGAAGTAAT TGAGATCCAG GCCCGCTCCT CTCTGGGGAC CCCCGCCCGA 
GCATATTTGG AAATCCCCAA CCCACGCTAC CTGGGCCCTG CAATCTCCTC AGGAGCCATC 
TACCTGGCGT CCTCATATCA GGATAAGTTA AGGGTCATCT GCTGCAAAGG AAACCTGGTG 
AAGGAGACCG GCACCGACCA CCACCGTGGC CCATCCACCT CCCGCAGCCC CAACAAGCGA 
GGCCCGCCGA CGTACAACGA GCACATCACC AAGCGCGTGG CCTCAAGCCC GGCGCCTCCC 
GAAGGCCCCA GCCACCCTCG AGAGCCAAGC ACACCCCACC GCTACCGCGA GGGGCGGACC 
GAGCTGCGCA GGGACAAGTC TCCGGGCCGC CCCCTGGAGC GCGAGAAGTC CCCCGGCCGG 
ATGCTGAGCA CGCGGAGGGA GCGCTCCCCC GGGCGGCTGT TTGAGGACAG CAGCAGGGGC 
CGGCTGCCCG TGGGGGCCGT GAGGACCCCG CTGTCCCAGG TCAACAAGGT CTGGGACCAG 
TCTTCAGTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CIT ( XM_024977735.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024833503.1 Bos taurus citron rho-interacting serine/threonine kinase (CIT), transcript variant X3, mRNA.

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CloneID	OBa224623
Clone ID Related Accession (Same CDS sequence)	XM_024977735.1
Accession Version	XM_024977735.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6207bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	citron Rho-interacting kinase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGAAGT TCAAGTATGG AGCACGGAAT CTACTGGACG CTGGTACTGT TGAATCCATT 
GCCAGCCGGG CCTCCAGGCT GAATCTTTTC TTCCAGGGGA AACCACCCTC CATGACTCAA 
CAGCAGATGT CTCTTCTTTC CCGAGAAGCG ATATTAGATG CCCTCTTTGT TCTCTTTAAA 
GAATGCAGTC AGCCAGCTCT GATGAAGATT AAGCACGTGA GCAACTTTGT CCAAAAGTAT 
TCCGATATCA TAGCTGAGTT GCAAGAGCTC CAGCCTTCAG TGAAGGACTT TGAAGTGAAA 
AGTGTCGTAG GCTGCGGTCA CTTTGCTGAA GTACAGGTGG TCAGAGAGAA AGCGACTGGG 
GATGTCTATG CCATGAAAGT CATGAAGAAG AAGGCCTTGT TGGCCCAGGA GCAGGTCTCA 
TATTTTGAGG AAGAACAGAA CATATTATCT CAGAGTACAA GTCCTTGGAT CCCCCAGTTG 
CAGTATGCCT TTCAGGACAA AAATAACCTT TATCTGGTCA TGGAATATCA GCCTGGAGGG 
GATTTGCTGT CACTTTTGAA TAGATATGAG GACCAATTAG ATGAAAATAT GATTCAGTTT 
TACCTAGCTG AACTGATTTT GGCTGTCCAC AGCGTTCATC AGATGGGCTA TGTACATCGA 
GACATCAAGC CCGAGAACAT CCTCATTGAC CGAACGGGAC ACATCAAGCT GGTGGATTTT 
GGATCAGCTG CTAAAATGAA CTCAAATAAG ATGGTAAATG CCAAACTCCC GGTTGGTACT 
CCAGATTACA TGGCCCCTGA AGTGTTGACT GTGATGAATG GGGATGGAAA AGGTGCCTAT 
AGCCTAGACT GTGATTGGTG GTCAGTGGGA GTTATTGCTT ATGAGATGGT TTATGGAAGA 
TGCCCATTCA CTGAAGGAAC CTCAGCCAAA ACCTTCAATA ACATCATGAA TTTCCAGCGG 
TTCTTGAAGT TTCCAGATGA CCCCAAAGTT AGCAGTGAAT TGCTTGATCT AATCCAGAGT 
TTGTTGTGTG GCCAGAAAGA GAGACTGAAG TTTGAGGGCC TTTGCTGTCA TCCTTTCTTC 
TCCAAAATTG ACTGGATTAA CATTCGTAAC TCTCCTCCCC CCTTCGTTCC CACCCTCAAG 
TCTGATGATG ACACCTCCAA TTTTGATGAA CCAGAGAAGA ATTCGTGGGT TTCATCCTCT 
CCGTGCCAGC TGAACCCCTC AGGTTTCTCG GGCGAAGAAC TGCCGTTTGT GGGGTTTTCG 
TATAGCAAGG CACTGGGAAT TCTTGGTAGA TCTGAGCCTG TTGTATCAAG TCTGGACTCC 
CCTGCCAAGA CTAGCTCCAT GGAAAAGAAA CTCCTTATCA AAAGCAAAGA GCTGCAGGAC 
TCCCAGGACA AGTGTCACAA GATGGAGCAG GAAATGACCC GGTTACATCG GAGAGTGTCA 
GAGGTGGAGG CTGTGCTTAG TCAGAAGGAG GTGGAGCTGA AGGCCTCTGA GACTCAGAGA 
TCCCTCCTGG AGCAGGACCT TGCTACCTAC ATCACAGAAT GCAGTAGCTT AAAGCGAAGT 
TTGGAGCAAG CACGGATGGA GGTGTCCCAA GAGGACGACA AAGCACTGCA GCTCCTCCAT 
GATATCAGAG AGCAGAGCCG GAAGCTCCAA GAAATCAAAG AGCAGGAGTA CCAGGCTCAA 
GTGGAAGAAA TGAGGTTGAT GATGAATCAG TTGGAAGAGG ACCTGGTTTC GGCGAGAAGG 
CGGAGTGACC TCTATGAATC GGAGCTCAGA GAGTCTCGGC TGGCTGCTGA AGAGTTTAAG 
CGGAAAGCAA CAGAATGTCA GCATAAACTG ATGAAGGCTA AAGATCAAGG GAAGCCTGAA 
GTGGGAGAAT ATGCCAAACT GGAGAAGATC AATGCTGAGC AGCAGCTCAA AATTCAGGAG 
CTCCAAGAGA AGCTAGAAAA GGCTGTGAAA GCTAGCACAG AGGCCACCGA GCTGCTGCAG 
AACATCCGTC AGGCGAAGGA GCGGGCCGAG CGTGAGCTGG AGAAGCTGCA CAACCGTGAG 
GACTCCTCCG AGGGCATCAA GAAGAAGCTG GTGGAGGCCG AGGAACGCCG CCACTCTCTG 
GAGAACAAGG TAAAGAGGCT AGAGACCATG GAGCGTAGAG AAAACAGACT GAAGGATGAC 
ATCCAGACAA AGTCCCAACA GATCCAGCAG ATGGCTGATA AAATTCTGGA GCTGGAGGAG 
AAGCACAGAG AAGCCCAGGT CTCAGCGCAG CATCTGGAGG TGCACCTGAA ACAGAAAGAG 
CAGCACTATG AAGAAAAAAT TAAAGTGTTG GACAATCAGA TAAAGAAAGA CCTAGCAGAC 
AAGGAGACAC TGGAGAACCT GATGCAAAGA CACGAAGAGG AGGCCCATGA GAAAGGCAAA 
ATTCTCAGCG AGCAGAAGGC GATGATCAAT GCTATGGATT CCAAGATCAG ATCCCTGGAA 
CAAAGGATAG TGGAATTGTC GGAAGCCAAT AAACTTGCGG CCAACAGCAG TCTCTTTACC 
CAGAGGAACA TGAAGGCCCA GGAAGAGATG ATTTCAGAAC TCAGGCAACA GAAGTTTTAC 
CTGGAGACGC AAGCTGGGAA ATTGGAGGCC CAGAACCGGA AGCTGGAGGA GCAGCTGGAA 
AAAATAAGCC ACCAAGACCA CAGTGACAAG AACCGTCTGC TGGAGCTGGA GACCAGGCTA 
AGGGAGGTCA GTCTAGAACA TGAAGAGCAG AAACTGGAGC TCAAACGCCA GCTCACAGAG 
CTGCAGCTCT CCCTGCAGGA GCGTGAATCC CAGCTGACAG CCCTGCAGGC AGCACGGGCG 
GCCCTGGAGA GCCAGCTCCG CCAGGCCAAG ACGGAGCTGG AAGAGACGAC AGCAGAAGCA 
GAAGAAGAGA TCCAGGCGCT TACGGCACAT AGAGATGAAA TCCAGCGCAA ATTCGATGCC 
CTTCGTAACA GCTGTACTGT GATCACGGAC TTGGAGGAGC AGCTAAACCA GCTGACGGAG 
GACAATGCCG AGCTCAACAA CCAAAACTTC TACTTGTCTA AACAACTCGA TGAGGCCTCT 
GGTGCCAACG ACGAGATTGT GCAGCTGCGA AGTGAAGTGG ACCATCTCCG CCGCGAGATC 
ACTGAGAGGG AGATGCAGCT CACTAGCCAG AAGCAAACGA TGGAGGCGCT GAAGACCACG 
TGCACAATGC TGGAGGAGCA GGTCATGGAC CTGGAGGCCC TGAACGACGA GCTGCTGGAG 
AAGGAGCGGC AGTGGGAGGC CTGGAGGAGT GTCCTTGGTG ACGAGAAGTC CCAGTTTGAG 
TGTCGGGTTC GAGAGTTGCA GAGGATGCTG GACACCGAAA AGCAGAGCAG GGCGAGGGCC 
GACCAGAGGA TCACTGAGTC GCGCCAGGTG GTCGAGCTGG CAGTAAAGGA GCACAAGGCC 
GAGATCCTGG CCTTGCAGCA GGCTCTCAAG GAGCAGAAGC TGAAGGCCGA GAGCCTCTCC 
GACAAGCTCA ATGATCTGGA GAAGAAGCAC GCCATGCTTG AAATGAATGC CCGAAGTTTA 
CAACAGAAGC TGGAGACAGA ACGAGAGCTC AAACAAAGGC TTCTGGAAGA GCAAGCCAAG 
TTACAGCAGC AGATGGACCT GCAGAAGAAT CATATTTTCC GTCTGACTCA AGGGCTGCAA 
GAAGCTCTGG ATCGGGCTGA TCTGCTGAAG ACAGAAAGGA GTGACCTGGA ATATCAGCTG 
GAAAACATTC AGGTTCTCTA TTCTCATGAG AAGGTGAAGA TGGAAGGCAC TATTTCCCAG 
CAAACCAAAC TCATCGATTT TCTGCAAGCC AAAATGGACC AGCCTGCCAA AAAGAAAAAG 
GGTTTATTTA GTCGACGGAA AGAGGACCCT GCTTTGCCCA CACAGGTTCC TCTGCAGTAC 
AATGAGCTGA AGGTGGCTCT GGAGAAGGAG AAAGCTCGCT GCGCGGAGCT CGAGGAAGCC 
CTGCAGAAGA CCCGCATCGA GCTCCGCTCC GCCCGGGAGG AAGCTGCCCA CCGGAAAGCT 
GCAGACCACC CACACCCATC TACGCCGGCT ACCGCCAGGC AGCAGATCGC CATGTCCGCC 
ATTGTGCGGT CACCCGAGCA CCAGCCCAGC GCCCTGAGCC TGCTCGCCCC GCCGTCCAGC 
CGCAGGAAGG AGTCTTCCAC TCCAGAGGAA TTCAGCCGGC GTCTTAAGGA GCGCATGCAC 
CACAACATCC CTCACCGGTT CAACGTGGGA CTGAACATGC GAGCCACGAA GTGCGCCGTG 
TGTCTGGATA CCGTGCACTT CGGGCGCCAG GCATCCAAGT GTCTCGAATG TCAGGTCATG 
TGCCACCCCA AGTGCTCCAC ATGCTTGCCA GCCACCTGTG GCCTGCCAGC CGAATACGCC 
ACACACTTCA CCGAGGCCTT CTGCCGCGAC AAAATGAACT CCCCGGGTCT GCAGTCCAAG 
GAGCCCAGCA GCAGCCTGCA CCTGGAAGGG TGGATGAAGG TGCCCAGGAA TAACAAGCGA 
GGACAGCAAG GCTGGGACAG GAAGTACATC GTTCTGGAGG GCTCCAAAGT CCTCATTTAT 
GACAACGAAG CCAGAGAAGC TGGACAGAGG CCGGTGGAAG AATTTGAGCT GTGCCTTCCC 
GACGGGGACG TGTCTATTCA TGGCGCCGTT GGTGCTTCTG AACTTGCAAA CACAGCCAAA 
GCAGATGTCC CCTATGTCCT GAAGATGGAG TCTCACCCCC ATACCACCTG CTGGCCTGGG 
AGGACCCTCT ACTTGCTGGC TCCCAGCTTT CCCGACAAGC AGCGCTGGGT CACCGCCTTA 
GAGTCAGTTG TGGCAGGTGG GAGAGTGTCT AGGGAAAAGG CGGAAGCCGA CGCTAAATTG 
CTCGGAAACT CCCTGTTGAA ACTGGAAGGT GATGACCGTC TGGACATGAA CTGCACGCTG 
CCCTTCAGCG ACCAGGTGGT GCTGGTGGGC ACCGAGGAAG GGCTGTATGC GCTGAATGTC 
TTGAAGAACT CCCTCACCCA CGTCCCAGGA ATCGGAGCAG TCTTCCAGAT TTACATCATC 
AAGGACCTGG AGAAGCTCCT CATGATCGCA GGAGAGGAGC GGGCCCTGTG TCTCGTGGAT 
GTGAAGAAGG TGAAGCAGTC CCTCGCGCAG TCTCACCTCC CCGCCCAGCC AGACATCTCG 
CCCAACATCT TTGAAGCGGT CAAGGGCTGC CACTTGTTTG CTGCTGGCAA GATTGAGAGC 
GGGCTCTGCA TCTGTGCAGC CATGCCCAGC AAAGTTGTCA TTCTCCGCTA CAATGAAAAC 
CTCAGCAAGT ACTGCATTCG CAAAGAGATC GAGACCTCGG AGCCCTGCAG CTGTATCCAC 
TTCACCAACT ACAGTATCCT CATCGGAACC AATAAATTCT ACGAAATCGA CATGAAGCAG 
TACACGCTAG AGGAATTCCT GGATAAGAAC GACCATTCCT TGGCGCCTGC CGTGTTTGCC 
TCCTCTTCCA ACAGCTTCCC CGTGTCCATC ATGCAGGTGA ACGGCGCGGG GCAGCGGGAG 
GAGTTCCTGC TCTGTTTCCA CGAATTCGGG GTGTTCGTGG ATTCTTATGG AAGACGCAGC 
CGCACAGATG ATCTCAAGTG GAGTCGCTTA CCTCTGGCCT TTGCCTACAG AGAACCCTAT 
CTGTTTGTGA CTCACTTCAA CTCACTCGAA GTAATTGAGA TCCAGGCCCG CTCCTCTCTG 
GGGACCCCCG CCCGAGCATA TTTGGAAATC CCCAACCCAC GCTACCTGGG CCCTGCAATC 
TCCTCAGGAG CCATCTACCT GGCGTCCTCA TATCAGGATA AGTTAAGGGT CATCTGCTGC 
AAAGGAAACC TGGTGAAGGA GACCGGCACC GACCACCACC GTGGCCCATC CACCTCCCGC 
AGCCCCAACA AGCGAGGCCC GCCGACGTAC AACGAGCACA TCACCAAGCG CGTGGCCTCA 
AGCCCGGCGC CTCCCGAAGG CCCCAGCCAC CCTCGAGAGC CAAGCACACC CCACCGCTAC 
CGCGAGGGGC GGACCGAGCT GCGCAGGGAC AAGTCTCCGG GCCGCCCCCT GGAGCGCGAG 
AAGTCCCCCG GCCGGATGCT GAGCACGCGG AGGGAGCGCT CCCCCGGGCG GCTGTTTGAG 
GACAGCAGCA GGGGCCGGCT GCCCGTGGGG GCCGTGAGGA CCCCGCTGTC CCAGGTCAAC 
AAGGTCTGGG ACCAGTCTTC AGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024833503.1
CDS	113..6319
Translation	MLKFKYGARNLLDAGTVESIASRASRLNLFFQGKPPSMTQQQMSLLSREAILDALFVLFKECSQPALMKIKHVSNFVQKYSDIIAELQELQPSVKDFEVKSVVGCGHFAEVQVVREKATGDVYAMKVMKKKALLAQEQVSYFEEEQNILSQSTSPWIPQLQYAFQDKNNLYLVMEYQPGGDLLSLLNRYEDQLDENMIQFYLAELILAVHSVHQMGYVHRDIKPENILIDRTGHIKLVDFGSAAKMNSNKMVNAKLPVGTPDYMAPEVLTVMNGDGKGAYSLDCDWWSVGVIAYEMVYGRCPFTEGTSAKTFNNIMNFQRFLKFPDDPKVSSELLDLIQSLLCGQKERLKFEGLCCHPFFSKIDWINIRNSPPPFVPTLKSDDDTSNFDEPEKNSWVSSSPCQLNPSGFSGEELPFVGFSYSKALGILGRSEPVVSSLDSPAKTSSMEKKLLIKSKELQDSQDKCHKMEQEMTRLHRRVSEVEAVLSQKEVELKASETQRSLLEQDLATYITECSSLKRSLEQARMEVSQEDDKALQLLHDIREQSRKLQEIKEQEYQAQVEEMRLMMNQLEEDLVSARRRSDLYESELRESRLAAEEFKRKATECQHKLMKAKDQGKPEVGEYAKLEKINAEQQLKIQELQEKLEKAVKASTEATELLQNIRQAKERAERELEKLHNREDSSEGIKKKLVEAEERRHSLENKVKRLETMERRENRLKDDIQTKSQQIQQMADKILELEEKHREAQVSAQHLEVHLKQKEQHYEEKIKVLDNQIKKDLADKETLENLMQRHEEEAHEKGKILSEQKAMINAMDSKIRSLEQRIVELSEANKLAANSSLFTQRNMKAQEEMISELRQQKFYLETQAGKLEAQNRKLEEQLEKISHQDHSDKNRLLELETRLREVSLEHEEQKLELKRQLTELQLSLQERESQLTALQAARAALESQLRQAKTELEETTAEAEEEIQALTAHRDEIQRKFDALRNSCTVITDLEEQLNQLTEDNAELNNQNFYLSKQLDEASGANDEIVQLRSEVDHLRREITEREMQLTSQKQTMEALKTTCTMLEEQVMDLEALNDELLEKERQWEAWRSVLGDEKSQFECRVRELQRMLDTEKQSRARADQRITESRQVVELAVKEHKAEILALQQALKEQKLKAESLSDKLNDLEKKHAMLEMNARSLQQKLETERELKQRLLEEQAKLQQQMDLQKNHIFRLTQGLQEALDRADLLKTERSDLEYQLENIQVLYSHEKVKMEGTISQQTKLIDFLQAKMDQPAKKKKGLFSRRKEDPALPTQVPLQYNELKVALEKEKARCAELEEALQKTRIELRSAREEAAHRKAADHPHPSTPATARQQIAMSAIVRSPEHQPSALSLLAPPSSRRKESSTPEEFSRRLKERMHHNIPHRFNVGLNMRATKCAVCLDTVHFGRQASKCLECQVMCHPKCSTCLPATCGLPAEYATHFTEAFCRDKMNSPGLQSKEPSSSLHLEGWMKVPRNNKRGQQGWDRKYIVLEGSKVLIYDNEAREAGQRPVEEFELCLPDGDVSIHGAVGASELANTAKADVPYVLKMESHPHTTCWPGRTLYLLAPSFPDKQRWVTALESVVAGGRVSREKAEADAKLLGNSLLKLEGDDRLDMNCTLPFSDQVVLVGTEEGLYALNVLKNSLTHVPGIGAVFQIYIIKDLEKLLMIAGEERALCLVDVKKVKQSLAQSHLPAQPDISPNIFEAVKGCHLFAAGKIESGLCICAAMPSKVVILRYNENLSKYCIRKEIETSEPCSCIHFTNYSILIGTNKFYEIDMKQYTLEEFLDKNDHSLAPAVFASSSNSFPVSIMQVNGAGQREEFLLCFHEFGVFVDSYGRRSRTDDLKWSRLPLAFAYREPYLFVTHFNSLEVIEIQARSSLGTPARAYLEIPNPRYLGPAISSGAIYLASSYQDKLRVICCKGNLVKETGTDHHRGPSTSRSPNKRGPPTYNEHITKRVASSPAPPEGPSHPREPSTPHRYREGRTELRRDKSPGRPLEREKSPGRMLSTRRERSPGRLFEDSSRGRLPVGAVRTPLSQVNKVWDQSSV

Target ORF information:

RefSeq Version	XM_024977735.1
Organism	Bos taurus(cattle)
Definition	Bos taurus citron rho-interacting serine/threonine kinase (CIT), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024977735.1

ORF Insert Sequence:

ATGTTGAAGT TCAAGTATGG AGCACGGAAT CTACTGGACG CTGGTACTGT TGAATCCATT 
GCCAGCCGGG CCTCCAGGCT GAATCTTTTC TTCCAGGGGA AACCACCCTC CATGACTCAA 
CAGCAGATGT CTCTTCTTTC CCGAGAAGCG ATATTAGATG CCCTCTTTGT TCTCTTTAAA 
GAATGCAGTC AGCCAGCTCT GATGAAGATT AAGCACGTGA GCAACTTTGT CCAAAAGTAT 
TCCGATATCA TAGCTGAGTT GCAAGAGCTC CAGCCTTCAG TGAAGGACTT TGAAGTGAAA 
AGTGTCGTAG GCTGCGGTCA CTTTGCTGAA GTACAGGTGG TCAGAGAGAA AGCGACTGGG 
GATGTCTATG CCATGAAAGT CATGAAGAAG AAGGCCTTGT TGGCCCAGGA GCAGGTCTCA 
TATTTTGAGG AAGAACAGAA CATATTATCT CAGAGTACAA GTCCTTGGAT CCCCCAGTTG 
CAGTATGCCT TTCAGGACAA AAATAACCTT TATCTGGTCA TGGAATATCA GCCTGGAGGG 
GATTTGCTGT CACTTTTGAA TAGATATGAG GACCAATTAG ATGAAAATAT GATTCAGTTT 
TACCTAGCTG AACTGATTTT GGCTGTCCAC AGCGTTCATC AGATGGGCTA TGTACATCGA 
GACATCAAGC CCGAGAACAT CCTCATTGAC CGAACGGGAC ACATCAAGCT GGTGGATTTT 
GGATCAGCTG CTAAAATGAA CTCAAATAAG ATGGTAAATG CCAAACTCCC GGTTGGTACT 
CCAGATTACA TGGCCCCTGA AGTGTTGACT GTGATGAATG GGGATGGAAA AGGTGCCTAT 
AGCCTAGACT GTGATTGGTG GTCAGTGGGA GTTATTGCTT ATGAGATGGT TTATGGAAGA 
TGCCCATTCA CTGAAGGAAC CTCAGCCAAA ACCTTCAATA ACATCATGAA TTTCCAGCGG 
TTCTTGAAGT TTCCAGATGA CCCCAAAGTT AGCAGTGAAT TGCTTGATCT AATCCAGAGT 
TTGTTGTGTG GCCAGAAAGA GAGACTGAAG TTTGAGGGCC TTTGCTGTCA TCCTTTCTTC 
TCCAAAATTG ACTGGATTAA CATTCGTAAC TCTCCTCCCC CCTTCGTTCC CACCCTCAAG 
TCTGATGATG ACACCTCCAA TTTTGATGAA CCAGAGAAGA ATTCGTGGGT TTCATCCTCT 
CCGTGCCAGC TGAACCCCTC AGGTTTCTCG GGCGAAGAAC TGCCGTTTGT GGGGTTTTCG 
TATAGCAAGG CACTGGGAAT TCTTGGTAGA TCTGAGCCTG TTGTATCAAG TCTGGACTCC 
CCTGCCAAGA CTAGCTCCAT GGAAAAGAAA CTCCTTATCA AAAGCAAAGA GCTGCAGGAC 
TCCCAGGACA AGTGTCACAA GATGGAGCAG GAAATGACCC GGTTACATCG GAGAGTGTCA 
GAGGTGGAGG CTGTGCTTAG TCAGAAGGAG GTGGAGCTGA AGGCCTCTGA GACTCAGAGA 
TCCCTCCTGG AGCAGGACCT TGCTACCTAC ATCACAGAAT GCAGTAGCTT AAAGCGAAGT 
TTGGAGCAAG CACGGATGGA GGTGTCCCAA GAGGACGACA AAGCACTGCA GCTCCTCCAT 
GATATCAGAG AGCAGAGCCG GAAGCTCCAA GAAATCAAAG AGCAGGAGTA CCAGGCTCAA 
GTGGAAGAAA TGAGGTTGAT GATGAATCAG TTGGAAGAGG ACCTGGTTTC GGCGAGAAGG 
CGGAGTGACC TCTATGAATC GGAGCTCAGA GAGTCTCGGC TGGCTGCTGA AGAGTTTAAG 
CGGAAAGCAA CAGAATGTCA GCATAAACTG ATGAAGGCTA AAGATCAAGG GAAGCCTGAA 
GTGGGAGAAT ATGCCAAACT GGAGAAGATC AATGCTGAGC AGCAGCTCAA AATTCAGGAG 
CTCCAAGAGA AGCTAGAAAA GGCTGTGAAA GCTAGCACAG AGGCCACCGA GCTGCTGCAG 
AACATCCGTC AGGCGAAGGA GCGGGCCGAG CGTGAGCTGG AGAAGCTGCA CAACCGTGAG 
GACTCCTCCG AGGGCATCAA GAAGAAGCTG GTGGAGGCCG AGGAACGCCG CCACTCTCTG 
GAGAACAAGG TAAAGAGGCT AGAGACCATG GAGCGTAGAG AAAACAGACT GAAGGATGAC 
ATCCAGACAA AGTCCCAACA GATCCAGCAG ATGGCTGATA AAATTCTGGA GCTGGAGGAG 
AAGCACAGAG AAGCCCAGGT CTCAGCGCAG CATCTGGAGG TGCACCTGAA ACAGAAAGAG 
CAGCACTATG AAGAAAAAAT TAAAGTGTTG GACAATCAGA TAAAGAAAGA CCTAGCAGAC 
AAGGAGACAC TGGAGAACCT GATGCAAAGA CACGAAGAGG AGGCCCATGA GAAAGGCAAA 
ATTCTCAGCG AGCAGAAGGC GATGATCAAT GCTATGGATT CCAAGATCAG ATCCCTGGAA 
CAAAGGATAG TGGAATTGTC GGAAGCCAAT AAACTTGCGG CCAACAGCAG TCTCTTTACC 
CAGAGGAACA TGAAGGCCCA GGAAGAGATG ATTTCAGAAC TCAGGCAACA GAAGTTTTAC 
CTGGAGACGC AAGCTGGGAA ATTGGAGGCC CAGAACCGGA AGCTGGAGGA GCAGCTGGAA 
AAAATAAGCC ACCAAGACCA CAGTGACAAG AACCGTCTGC TGGAGCTGGA GACCAGGCTA 
AGGGAGGTCA GTCTAGAACA TGAAGAGCAG AAACTGGAGC TCAAACGCCA GCTCACAGAG 
CTGCAGCTCT CCCTGCAGGA GCGTGAATCC CAGCTGACAG CCCTGCAGGC AGCACGGGCG 
GCCCTGGAGA GCCAGCTCCG CCAGGCCAAG ACGGAGCTGG AAGAGACGAC AGCAGAAGCA 
GAAGAAGAGA TCCAGGCGCT TACGGCACAT AGAGATGAAA TCCAGCGCAA ATTCGATGCC 
CTTCGTAACA GCTGTACTGT GATCACGGAC TTGGAGGAGC AGCTAAACCA GCTGACGGAG 
GACAATGCCG AGCTCAACAA CCAAAACTTC TACTTGTCTA AACAACTCGA TGAGGCCTCT 
GGTGCCAACG ACGAGATTGT GCAGCTGCGA AGTGAAGTGG ACCATCTCCG CCGCGAGATC 
ACTGAGAGGG AGATGCAGCT CACTAGCCAG AAGCAAACGA TGGAGGCGCT GAAGACCACG 
TGCACAATGC TGGAGGAGCA GGTCATGGAC CTGGAGGCCC TGAACGACGA GCTGCTGGAG 
AAGGAGCGGC AGTGGGAGGC CTGGAGGAGT GTCCTTGGTG ACGAGAAGTC CCAGTTTGAG 
TGTCGGGTTC GAGAGTTGCA GAGGATGCTG GACACCGAAA AGCAGAGCAG GGCGAGGGCC 
GACCAGAGGA TCACTGAGTC GCGCCAGGTG GTCGAGCTGG CAGTAAAGGA GCACAAGGCC 
GAGATCCTGG CCTTGCAGCA GGCTCTCAAG GAGCAGAAGC TGAAGGCCGA GAGCCTCTCC 
GACAAGCTCA ATGATCTGGA GAAGAAGCAC GCCATGCTTG AAATGAATGC CCGAAGTTTA 
CAACAGAAGC TGGAGACAGA ACGAGAGCTC AAACAAAGGC TTCTGGAAGA GCAAGCCAAG 
TTACAGCAGC AGATGGACCT GCAGAAGAAT CATATTTTCC GTCTGACTCA AGGGCTGCAA 
GAAGCTCTGG ATCGGGCTGA TCTGCTGAAG ACAGAAAGGA GTGACCTGGA ATATCAGCTG 
GAAAACATTC AGGTTCTCTA TTCTCATGAG AAGGTGAAGA TGGAAGGCAC TATTTCCCAG 
CAAACCAAAC TCATCGATTT TCTGCAAGCC AAAATGGACC AGCCTGCCAA AAAGAAAAAG 
GGTTTATTTA GTCGACGGAA AGAGGACCCT GCTTTGCCCA CACAGGTTCC TCTGCAGTAC 
AATGAGCTGA AGGTGGCTCT GGAGAAGGAG AAAGCTCGCT GCGCGGAGCT CGAGGAAGCC 
CTGCAGAAGA CCCGCATCGA GCTCCGCTCC GCCCGGGAGG AAGCTGCCCA CCGGAAAGCT 
GCAGACCACC CACACCCATC TACGCCGGCT ACCGCCAGGC AGCAGATCGC CATGTCCGCC 
ATTGTGCGGT CACCCGAGCA CCAGCCCAGC GCCCTGAGCC TGCTCGCCCC GCCGTCCAGC 
CGCAGGAAGG AGTCTTCCAC TCCAGAGGAA TTCAGCCGGC GTCTTAAGGA GCGCATGCAC 
CACAACATCC CTCACCGGTT CAACGTGGGA CTGAACATGC GAGCCACGAA GTGCGCCGTG 
TGTCTGGATA CCGTGCACTT CGGGCGCCAG GCATCCAAGT GTCTCGAATG TCAGGTCATG 
TGCCACCCCA AGTGCTCCAC ATGCTTGCCA GCCACCTGTG GCCTGCCAGC CGAATACGCC 
ACACACTTCA CCGAGGCCTT CTGCCGCGAC AAAATGAACT CCCCGGGTCT GCAGTCCAAG 
GAGCCCAGCA GCAGCCTGCA CCTGGAAGGG TGGATGAAGG TGCCCAGGAA TAACAAGCGA 
GGACAGCAAG GCTGGGACAG GAAGTACATC GTTCTGGAGG GCTCCAAAGT CCTCATTTAT 
GACAACGAAG CCAGAGAAGC TGGACAGAGG CCGGTGGAAG AATTTGAGCT GTGCCTTCCC 
GACGGGGACG TGTCTATTCA TGGCGCCGTT GGTGCTTCTG AACTTGCAAA CACAGCCAAA 
GCAGATGTCC CCTATGTCCT GAAGATGGAG TCTCACCCCC ATACCACCTG CTGGCCTGGG 
AGGACCCTCT ACTTGCTGGC TCCCAGCTTT CCCGACAAGC AGCGCTGGGT CACCGCCTTA 
GAGTCAGTTG TGGCAGGTGG GAGAGTGTCT AGGGAAAAGG CGGAAGCCGA CGCTAAATTG 
CTCGGAAACT CCCTGTTGAA ACTGGAAGGT GATGACCGTC TGGACATGAA CTGCACGCTG 
CCCTTCAGCG ACCAGGTGGT GCTGGTGGGC ACCGAGGAAG GGCTGTATGC GCTGAATGTC 
TTGAAGAACT CCCTCACCCA CGTCCCAGGA ATCGGAGCAG TCTTCCAGAT TTACATCATC 
AAGGACCTGG AGAAGCTCCT CATGATCGCA GGAGAGGAGC GGGCCCTGTG TCTCGTGGAT 
GTGAAGAAGG TGAAGCAGTC CCTCGCGCAG TCTCACCTCC CCGCCCAGCC AGACATCTCG 
CCCAACATCT TTGAAGCGGT CAAGGGCTGC CACTTGTTTG CTGCTGGCAA GATTGAGAGC 
GGGCTCTGCA TCTGTGCAGC CATGCCCAGC AAAGTTGTCA TTCTCCGCTA CAATGAAAAC 
CTCAGCAAGT ACTGCATTCG CAAAGAGATC GAGACCTCGG AGCCCTGCAG CTGTATCCAC 
TTCACCAACT ACAGTATCCT CATCGGAACC AATAAATTCT ACGAAATCGA CATGAAGCAG 
TACACGCTAG AGGAATTCCT GGATAAGAAC GACCATTCCT TGGCGCCTGC CGTGTTTGCC 
TCCTCTTCCA ACAGCTTCCC CGTGTCCATC ATGCAGGTGA ACGGCGCGGG GCAGCGGGAG 
GAGTTCCTGC TCTGTTTCCA CGAATTCGGG GTGTTCGTGG ATTCTTATGG AAGACGCAGC 
CGCACAGATG ATCTCAAGTG GAGTCGCTTA CCTCTGGCCT TTGCCTACAG AGAACCCTAT 
CTGTTTGTGA CTCACTTCAA CTCACTCGAA GTAATTGAGA TCCAGGCCCG CTCCTCTCTG 
GGGACCCCCG CCCGAGCATA TTTGGAAATC CCCAACCCAC GCTACCTGGG CCCTGCAATC 
TCCTCAGGAG CCATCTACCT GGCGTCCTCA TATCAGGATA AGTTAAGGGT CATCTGCTGC 
AAAGGAAACC TGGTGAAGGA GACCGGCACC GACCACCACC GTGGCCCATC CACCTCCCGC 
AGCCCCAACA AGCGAGGCCC GCCGACGTAC AACGAGCACA TCACCAAGCG CGTGGCCTCA 
AGCCCGGCGC CTCCCGAAGG CCCCAGCCAC CCTCGAGAGC CAAGCACACC CCACCGCTAC 
CGCGAGGGGC GGACCGAGCT GCGCAGGGAC AAGTCTCCGG GCCGCCCCCT GGAGCGCGAG 
AAGTCCCCCG GCCGGATGCT GAGCACGCGG AGGGAGCGCT CCCCCGGGCG GCTGTTTGAG 
GACAGCAGCA GGGGCCGGCT GCCCGTGGGG GCCGTGAGGA CCCCGCTGTC CCAGGTCAAC 
AAGGTCTGGG ACCAGTCTTC AGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CIT ( XM_024977736.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024833504.1 Bos taurus citron rho-interacting serine/threonine kinase (CIT), transcript variant X4, mRNA.

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CloneID	OBa224624
Clone ID Related Accession (Same CDS sequence)	XM_024977736.1
Accession Version	XM_024977736.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6207bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	citron Rho-interacting kinase isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGAAGT TCAAGTATGG AGCACGGAAT CTACTGGACG CTGGTACTGT TGAATCCATT 
GCCAGCCGGG CCTCCAGGCT GAATCTTTTC TTCCAGGGGA AACCACCCTC CATGACTCAA 
CAGCAGATGT CTCTTCTTTC CCGAGAAGCG ATATTAGATG CCCTCTTTGT TCTCTTTAAA 
GAATGCAGTC AGCCAGCTCT GATGAAGATT AAGCACGTGA GCAACTTTGT CCAAAAGTAT 
TCCGATATCA TAGCTGAGTT GCAAGAGCTC CAGCCTTCAG TGAAGGACTT TGAAGTGAAA 
AGTGTCGTAG GCTGCGGTCA CTTTGCTGAA GTACAGGTGG TCAGAGAGAA AGCGACTGGG 
GATGTCTATG CCATGAAAGT CATGAAGAAG AAGGCCTTGT TGGCCCAGGA GCAGGTCTCA 
TATTTTGAGG AAGAACAGAA CATATTATCT CAGAGTACAA GTCCTTGGAT CCCCCAGTTG 
CAGTATGCCT TTCAGGACAA AAATAACCTT TATCTGGTCA TGGAATATCA GCCTGGAGGG 
GATTTGCTGT CACTTTTGAA TAGATATGAG GACCAATTAG ATGAAAATAT GATTCAGTTT 
TACCTAGCTG AACTGATTTT GGCTGTCCAC AGCGTTCATC AGATGGGCTA TGTACATCGA 
GACATCAAGC CCGAGAACAT CCTCATTGAC CGAACGGGAC ACATCAAGCT GGTGGATTTT 
GGATCAGCTG CTAAAATGAA CTCAAATAAG ATGGTAAATG CCAAACTCCC GGTTGGTACT 
CCAGATTACA TGGCCCCTGA AGTGTTGACT GTGATGAATG GGGATGGAAA AGGTGCCTAT 
AGCCTAGACT GTGATTGGTG GTCAGTGGGA GTTATTGCTT ATGAGATGGT TTATGGAAGA 
TGCCCATTCA CTGAAGGAAC CTCAGCCAAA ACCTTCAATA ACATCATGAA TTTCCAGCGG 
TTCTTGAAGT TTCCAGATGA CCCCAAAGTT AGCAGTGAAT TGCTTGATCT AATCCAGAGT 
TTGTTGTGTG GCCAGAAAGA GAGACTGAAG TTTGAGGGCC TTTGCTGTCA TCCTTTCTTC 
TCCAAAATTG ACTGGATTAA CATTCGTAAC TCTCCTCCCC CCTTCGTTCC CACCCTCAAG 
TCTGATGATG ACACCTCCAA TTTTGATGAA CCAGAGAAGA ATTCGTGGGT TTCATCCTCT 
CCGTGCCAGC TGAACCCCTC AGGTTTCTCG GGCGAAGAAC TGCCGTTTGT GGGGTTTTCG 
TATAGCAAGG CACTGGGAAT TCTTGGTAGA TCTGAGCCTG TTGTATCAAG TCTGGACTCC 
CCTGCCAAGA CTAGCTCCAT GGAAAAGAAA CTCCTTATCA AAAGCAAAGA GCTGCAGGAC 
TCCCAGGACA AGTGTCACAA GATGGAGCAG GAAATGACCC GGTTACATCG GAGAGTGTCA 
GAGGTGGAGG CTGTGCTTAG TCAGAAGGAG GTGGAGCTGA AGGCCTCTGA GACTCAGAGA 
TCCCTCCTGG AGCAGGACCT TGCTACCTAC ATCACAGAAT GCAGTAGCTT AAAGCGAAGT 
TTGGAGCAAG CACGGATGGA GGTGTCCCAA GAGGACGACA AAGCACTGCA GCTCCTCCAT 
GATATCAGAG AGCAGAGCCG GAAGCTCCAA GAAATCAAAG AGCAGGAGTA CCAGGCTCAA 
GTGGAAGAAA TGAGGTTGAT GATGAATCAG TTGGAAGAGG ACCTGGTTTC GGCGAGAAGG 
CGGAGTGACC TCTATGAATC GGAGCTCAGA GAGTCTCGGC TGGCTGCTGA AGAGTTTAAG 
CGGAAAGCAA CAGAATGTCA GCATAAACTG ATGAAGGCTA AAGATCAAGG GAAGCCTGAA 
GTGGGAGAAT ATGCCAAACT GGAGAAGATC AATGCTGAGC AGCAGCTCAA AATTCAGGAG 
CTCCAAGAGA AGCTAGAAAA GGCTGTGAAA GCTAGCACAG AGGCCACCGA GCTGCTGCAG 
AACATCCGTC AGGCGAAGGA GCGGGCCGAG CGTGAGCTGG AGAAGCTGCA CAACCGTGAG 
GACTCCTCCG AGGGCATCAA GAAGAAGCTG GTGGAGGCCG AGGAACGCCG CCACTCTCTG 
GAGAACAAGG TAAAGAGGCT AGAGACCATG GAGCGTAGAG AAAACAGACT GAAGGATGAC 
ATCCAGACAA AGTCCCAACA GATCCAGCAG ATGGCTGATA AAATTCTGGA GCTGGAGGAG 
AAGCACAGAG AAGCCCAGGT CTCAGCGCAG CATCTGGAGG TGCACCTGAA ACAGAAAGAG 
CAGCACTATG AAGAAAAAAT TAAAGTGTTG GACAATCAGA TAAAGAAAGA CCTAGCAGAC 
AAGGAGACAC TGGAGAACCT GATGCAAAGA CACGAAGAGG AGGCCCATGA GAAAGGCAAA 
ATTCTCAGCG AGCAGAAGGC GATGATCAAT GCTATGGATT CCAAGATCAG ATCCCTGGAA 
CAAAGGATAG TGGAATTGTC GGAAGCCAAT AAACTTGCGG CCAACAGCAG TCTCTTTACC 
CAGAGGAACA TGAAGGCCCA GGAAGAGATG ATTTCAGAAC TCAGGCAACA GAAGTTTTAC 
CTGGAGACGC AAGCTGGGAA ATTGGAGGCC CAGAACCGGA AGCTGGAGGA GCAGCTGGAA 
AAAATAAGCC ACCAAGACCA CAGTGACAAG AACCGTCTGC TGGAGCTGGA GACCAGGCTA 
AGGGAGGTCA GTCTAGAACA TGAAGAGCAG AAACTGGAGC TCAAACGCCA GCTCACAGAG 
CTGCAGCTCT CCCTGCAGGA GCGTGAATCC CAGCTGACAG CCCTGCAGGC AGCACGGGCG 
GCCCTGGAGA GCCAGCTCCG CCAGGCCAAG ACGGAGCTGG AAGAGACGAC AGCAGAAGCA 
GAAGAAGAGA TCCAGGCGCT TACGGCACAT AGAGATGAAA TCCAGCGCAA ATTCGATGCC 
CTTCGTAACA GCTGTACTGT GATCACGGAC TTGGAGGAGC AGCTAAACCA GCTGACGGAG 
GACAATGCCG AGCTCAACAA CCAAAACTTC TACTTGTCTA AACAACTCGA TGAGGCCTCT 
GGTGCCAACG ACGAGATTGT GCAGCTGCGA AGTGAAGTGG ACCATCTCCG CCGCGAGATC 
ACTGAGAGGG AGATGCAGCT CACTAGCCAG AAGCAAACGA TGGAGGCGCT GAAGACCACG 
TGCACAATGC TGGAGGAGCA GGTCATGGAC CTGGAGGCCC TGAACGACGA GCTGCTGGAG 
AAGGAGCGGC AGTGGGAGGC CTGGAGGAGT GTCCTTGGTG ACGAGAAGTC CCAGTTTGAG 
TGTCGGGTTC GAGAGTTGCA GAGGATGCTG GACACCGAAA AGCAGAGCAG GGCGAGGGCC 
GACCAGAGGA TCACTGAGTC GCGCCAGGTG GTCGAGCTGG CAGTAAAGGA GCACAAGGCC 
GAGATCCTGG CCTTGCAGCA GGCTCTCAAG GAGCAGAAGC TGAAGGCCGA GAGCCTCTCC 
GACAAGCTCA ATGATCTGGA GAAGAAGCAC GCCATGCTTG AAATGAATGC CCGAAGTTTA 
CAACAGAAGC TGGAGACAGA ACGAGAGCTC AAACAAAGGC TTCTGGAAGA GCAAGCCAAG 
TTACAGCAGC AGATGGACCT GCAGAAGAAT CATATTTTCC GTCTGACTCA AGGGCTGCAA 
GAAGCTCTGG ATCGGGCTGA TCTGCTGAAG ACAGAAAGGA GTGACCTGGA ATATCAGCTG 
GAAAACATTC AGGTTCTCTA TTCTCATGAG AAGGTGAAGA TGGAAGGCAC TATTTCCCAG 
CAAACCAAAC TCATCGATTT TCTGCAAGCC AAAATGGACC AGCCTGCCAA AAAGAAAAAG 
GTTCCTCTGC AGTACAATGA GCTGAAGGTG GCTCTGGAGA AGGAGAAAGC TCGCTGCGCG 
GAGCTCGAGG AAGCCCTGCA GAAGACCCGC ATCGAGCTCC GCTCCGCCCG GGAGGAAGCT 
GCCCACCGGA AAGCTGCAGA CCACCCACAC CCATCTACGC CGGCTACCGC CAGGCAGCAG 
ATCGCCATGT CCGCCATTGT GCGGTCACCC GAGCACCAGC CCAGCGCCCT GAGCCTGCTC 
GCCCCGCCGT CCAGCCGCAG GAAGGAGTCT TCCACTCCAG AGGAATTCAG CCGGCGTCTT 
AAGGAGCGCA TGCACCACAA CATCCCTCAC CGGTTCAACG TGGGACTGAA CATGCGAGCC 
ACGAAGTGCG CCGTGTGTCT GGATACCGTG CACTTCGGGC GCCAGGCATC CAAGTGTCTC 
GAATGTCAGG TCATGTGCCA CCCCAAGTGC TCCACATGCT TGCCAGCCAC CTGTGGCCTG 
CCAGCCGAAT ACGCCACACA CTTCACCGAG GCCTTCTGCC GCGACAAAAT GAACTCCCCG 
GGTCTGCAGT CCAAGGAGCC CAGCAGCAGC CTGCACCTGG AAGGGTGGAT GAAGGTGCCC 
AGGAATAACA AGCGAGGACA GCAAGGCTGG GACAGGAAGT ACATCGTTCT GGAGGGCTCC 
AAAGTCCTCA TTTATGACAA CGAAGCCAGA GAAGCTGGAC AGAGGCCGGT GGAAGAATTT 
GAGCTGTGCC TTCCCGACGG GGACGTGTCT ATTCATGGCG CCGTTGGTGC TTCTGAACTT 
GCAAACACAG CCAAAGCAGA TGTCCCCTAT GTCCTGAAGA TGGAGTCTCA CCCCCATACC 
ACCTGCTGGC CTGGGAGGAC CCTCTACTTG CTGGCTCCCA GCTTTCCCGA CAAGCAGCGC 
TGGGTCACCG CCTTAGAGTC AGTTGTGGCA GGTGGGAGAG TGTCTAGGGA AAAGGCGGAA 
GCCGACGCTG GCCGTGACTG TACTTCCTAC GAGCCTCTGC CCGCGTGGGC TCAGAAATTG 
CTCGGAAACT CCCTGTTGAA ACTGGAAGGT GATGACCGTC TGGACATGAA CTGCACGCTG 
CCCTTCAGCG ACCAGGTGGT GCTGGTGGGC ACCGAGGAAG GGCTGTATGC GCTGAATGTC 
TTGAAGAACT CCCTCACCCA CGTCCCAGGA ATCGGAGCAG TCTTCCAGAT TTACATCATC 
AAGGACCTGG AGAAGCTCCT CATGATCGCA GGAGAGGAGC GGGCCCTGTG TCTCGTGGAT 
GTGAAGAAGG TGAAGCAGTC CCTCGCGCAG TCTCACCTCC CCGCCCAGCC AGACATCTCG 
CCCAACATCT TTGAAGCGGT CAAGGGCTGC CACTTGTTTG CTGCTGGCAA GATTGAGAGC 
GGGCTCTGCA TCTGTGCAGC CATGCCCAGC AAAGTTGTCA TTCTCCGCTA CAATGAAAAC 
CTCAGCAAGT ACTGCATTCG CAAAGAGATC GAGACCTCGG AGCCCTGCAG CTGTATCCAC 
TTCACCAACT ACAGTATCCT CATCGGAACC AATAAATTCT ACGAAATCGA CATGAAGCAG 
TACACGCTAG AGGAATTCCT GGATAAGAAC GACCATTCCT TGGCGCCTGC CGTGTTTGCC 
TCCTCTTCCA ACAGCTTCCC CGTGTCCATC ATGCAGGTGA ACGGCGCGGG GCAGCGGGAG 
GAGTTCCTGC TCTGTTTCCA CGAATTCGGG GTGTTCGTGG ATTCTTATGG AAGACGCAGC 
CGCACAGATG ATCTCAAGTG GAGTCGCTTA CCTCTGGCCT TTGCCTACAG AGAACCCTAT 
CTGTTTGTGA CTCACTTCAA CTCACTCGAA GTAATTGAGA TCCAGGCCCG CTCCTCTCTG 
GGGACCCCCG CCCGAGCATA TTTGGAAATC CCCAACCCAC GCTACCTGGG CCCTGCAATC 
TCCTCAGGAG CCATCTACCT GGCGTCCTCA TATCAGGATA AGTTAAGGGT CATCTGCTGC 
AAAGGAAACC TGGTGAAGGA GACCGGCACC GACCACCACC GTGGCCCATC CACCTCCCGC 
AGCCCCAACA AGCGAGGCCC GCCGACGTAC AACGAGCACA TCACCAAGCG CGTGGCCTCA 
AGCCCGGCGC CTCCCGAAGG CCCCAGCCAC CCTCGAGAGC CAAGCACACC CCACCGCTAC 
CGCGAGGGGC GGACCGAGCT GCGCAGGGAC AAGTCTCCGG GCCGCCCCCT GGAGCGCGAG 
AAGTCCCCCG GCCGGATGCT GAGCACGCGG AGGGAGCGCT CCCCCGGGCG GCTGTTTGAG 
GACAGCAGCA GGGGCCGGCT GCCCGTGGGG GCCGTGAGGA CCCCGCTGTC CCAGGTCAAC 
AAGGTCTGGG ACCAGTCTTC AGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024833504.1
CDS	113..6319
Translation	MLKFKYGARNLLDAGTVESIASRASRLNLFFQGKPPSMTQQQMSLLSREAILDALFVLFKECSQPALMKIKHVSNFVQKYSDIIAELQELQPSVKDFEVKSVVGCGHFAEVQVVREKATGDVYAMKVMKKKALLAQEQVSYFEEEQNILSQSTSPWIPQLQYAFQDKNNLYLVMEYQPGGDLLSLLNRYEDQLDENMIQFYLAELILAVHSVHQMGYVHRDIKPENILIDRTGHIKLVDFGSAAKMNSNKMVNAKLPVGTPDYMAPEVLTVMNGDGKGAYSLDCDWWSVGVIAYEMVYGRCPFTEGTSAKTFNNIMNFQRFLKFPDDPKVSSELLDLIQSLLCGQKERLKFEGLCCHPFFSKIDWINIRNSPPPFVPTLKSDDDTSNFDEPEKNSWVSSSPCQLNPSGFSGEELPFVGFSYSKALGILGRSEPVVSSLDSPAKTSSMEKKLLIKSKELQDSQDKCHKMEQEMTRLHRRVSEVEAVLSQKEVELKASETQRSLLEQDLATYITECSSLKRSLEQARMEVSQEDDKALQLLHDIREQSRKLQEIKEQEYQAQVEEMRLMMNQLEEDLVSARRRSDLYESELRESRLAAEEFKRKATECQHKLMKAKDQGKPEVGEYAKLEKINAEQQLKIQELQEKLEKAVKASTEATELLQNIRQAKERAERELEKLHNREDSSEGIKKKLVEAEERRHSLENKVKRLETMERRENRLKDDIQTKSQQIQQMADKILELEEKHREAQVSAQHLEVHLKQKEQHYEEKIKVLDNQIKKDLADKETLENLMQRHEEEAHEKGKILSEQKAMINAMDSKIRSLEQRIVELSEANKLAANSSLFTQRNMKAQEEMISELRQQKFYLETQAGKLEAQNRKLEEQLEKISHQDHSDKNRLLELETRLREVSLEHEEQKLELKRQLTELQLSLQERESQLTALQAARAALESQLRQAKTELEETTAEAEEEIQALTAHRDEIQRKFDALRNSCTVITDLEEQLNQLTEDNAELNNQNFYLSKQLDEASGANDEIVQLRSEVDHLRREITEREMQLTSQKQTMEALKTTCTMLEEQVMDLEALNDELLEKERQWEAWRSVLGDEKSQFECRVRELQRMLDTEKQSRARADQRITESRQVVELAVKEHKAEILALQQALKEQKLKAESLSDKLNDLEKKHAMLEMNARSLQQKLETERELKQRLLEEQAKLQQQMDLQKNHIFRLTQGLQEALDRADLLKTERSDLEYQLENIQVLYSHEKVKMEGTISQQTKLIDFLQAKMDQPAKKKKVPLQYNELKVALEKEKARCAELEEALQKTRIELRSAREEAAHRKAADHPHPSTPATARQQIAMSAIVRSPEHQPSALSLLAPPSSRRKESSTPEEFSRRLKERMHHNIPHRFNVGLNMRATKCAVCLDTVHFGRQASKCLECQVMCHPKCSTCLPATCGLPAEYATHFTEAFCRDKMNSPGLQSKEPSSSLHLEGWMKVPRNNKRGQQGWDRKYIVLEGSKVLIYDNEAREAGQRPVEEFELCLPDGDVSIHGAVGASELANTAKADVPYVLKMESHPHTTCWPGRTLYLLAPSFPDKQRWVTALESVVAGGRVSREKAEADAGRDCTSYEPLPAWAQKLLGNSLLKLEGDDRLDMNCTLPFSDQVVLVGTEEGLYALNVLKNSLTHVPGIGAVFQIYIIKDLEKLLMIAGEERALCLVDVKKVKQSLAQSHLPAQPDISPNIFEAVKGCHLFAAGKIESGLCICAAMPSKVVILRYNENLSKYCIRKEIETSEPCSCIHFTNYSILIGTNKFYEIDMKQYTLEEFLDKNDHSLAPAVFASSSNSFPVSIMQVNGAGQREEFLLCFHEFGVFVDSYGRRSRTDDLKWSRLPLAFAYREPYLFVTHFNSLEVIEIQARSSLGTPARAYLEIPNPRYLGPAISSGAIYLASSYQDKLRVICCKGNLVKETGTDHHRGPSTSRSPNKRGPPTYNEHITKRVASSPAPPEGPSHPREPSTPHRYREGRTELRRDKSPGRPLEREKSPGRMLSTRRERSPGRLFEDSSRGRLPVGAVRTPLSQVNKVWDQSSV

Target ORF information:

RefSeq Version	XM_024977736.1
Organism	Bos taurus(cattle)
Definition	Bos taurus citron rho-interacting serine/threonine kinase (CIT), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024977736.1

ORF Insert Sequence:

ATGTTGAAGT TCAAGTATGG AGCACGGAAT CTACTGGACG CTGGTACTGT TGAATCCATT 
GCCAGCCGGG CCTCCAGGCT GAATCTTTTC TTCCAGGGGA AACCACCCTC CATGACTCAA 
CAGCAGATGT CTCTTCTTTC CCGAGAAGCG ATATTAGATG CCCTCTTTGT TCTCTTTAAA 
GAATGCAGTC AGCCAGCTCT GATGAAGATT AAGCACGTGA GCAACTTTGT CCAAAAGTAT 
TCCGATATCA TAGCTGAGTT GCAAGAGCTC CAGCCTTCAG TGAAGGACTT TGAAGTGAAA 
AGTGTCGTAG GCTGCGGTCA CTTTGCTGAA GTACAGGTGG TCAGAGAGAA AGCGACTGGG 
GATGTCTATG CCATGAAAGT CATGAAGAAG AAGGCCTTGT TGGCCCAGGA GCAGGTCTCA 
TATTTTGAGG AAGAACAGAA CATATTATCT CAGAGTACAA GTCCTTGGAT CCCCCAGTTG 
CAGTATGCCT TTCAGGACAA AAATAACCTT TATCTGGTCA TGGAATATCA GCCTGGAGGG 
GATTTGCTGT CACTTTTGAA TAGATATGAG GACCAATTAG ATGAAAATAT GATTCAGTTT 
TACCTAGCTG AACTGATTTT GGCTGTCCAC AGCGTTCATC AGATGGGCTA TGTACATCGA 
GACATCAAGC CCGAGAACAT CCTCATTGAC CGAACGGGAC ACATCAAGCT GGTGGATTTT 
GGATCAGCTG CTAAAATGAA CTCAAATAAG ATGGTAAATG CCAAACTCCC GGTTGGTACT 
CCAGATTACA TGGCCCCTGA AGTGTTGACT GTGATGAATG GGGATGGAAA AGGTGCCTAT 
AGCCTAGACT GTGATTGGTG GTCAGTGGGA GTTATTGCTT ATGAGATGGT TTATGGAAGA 
TGCCCATTCA CTGAAGGAAC CTCAGCCAAA ACCTTCAATA ACATCATGAA TTTCCAGCGG 
TTCTTGAAGT TTCCAGATGA CCCCAAAGTT AGCAGTGAAT TGCTTGATCT AATCCAGAGT 
TTGTTGTGTG GCCAGAAAGA GAGACTGAAG TTTGAGGGCC TTTGCTGTCA TCCTTTCTTC 
TCCAAAATTG ACTGGATTAA CATTCGTAAC TCTCCTCCCC CCTTCGTTCC CACCCTCAAG 
TCTGATGATG ACACCTCCAA TTTTGATGAA CCAGAGAAGA ATTCGTGGGT TTCATCCTCT 
CCGTGCCAGC TGAACCCCTC AGGTTTCTCG GGCGAAGAAC TGCCGTTTGT GGGGTTTTCG 
TATAGCAAGG CACTGGGAAT TCTTGGTAGA TCTGAGCCTG TTGTATCAAG TCTGGACTCC 
CCTGCCAAGA CTAGCTCCAT GGAAAAGAAA CTCCTTATCA AAAGCAAAGA GCTGCAGGAC 
TCCCAGGACA AGTGTCACAA GATGGAGCAG GAAATGACCC GGTTACATCG GAGAGTGTCA 
GAGGTGGAGG CTGTGCTTAG TCAGAAGGAG GTGGAGCTGA AGGCCTCTGA GACTCAGAGA 
TCCCTCCTGG AGCAGGACCT TGCTACCTAC ATCACAGAAT GCAGTAGCTT AAAGCGAAGT 
TTGGAGCAAG CACGGATGGA GGTGTCCCAA GAGGACGACA AAGCACTGCA GCTCCTCCAT 
GATATCAGAG AGCAGAGCCG GAAGCTCCAA GAAATCAAAG AGCAGGAGTA CCAGGCTCAA 
GTGGAAGAAA TGAGGTTGAT GATGAATCAG TTGGAAGAGG ACCTGGTTTC GGCGAGAAGG 
CGGAGTGACC TCTATGAATC GGAGCTCAGA GAGTCTCGGC TGGCTGCTGA AGAGTTTAAG 
CGGAAAGCAA CAGAATGTCA GCATAAACTG ATGAAGGCTA AAGATCAAGG GAAGCCTGAA 
GTGGGAGAAT ATGCCAAACT GGAGAAGATC AATGCTGAGC AGCAGCTCAA AATTCAGGAG 
CTCCAAGAGA AGCTAGAAAA GGCTGTGAAA GCTAGCACAG AGGCCACCGA GCTGCTGCAG 
AACATCCGTC AGGCGAAGGA GCGGGCCGAG CGTGAGCTGG AGAAGCTGCA CAACCGTGAG 
GACTCCTCCG AGGGCATCAA GAAGAAGCTG GTGGAGGCCG AGGAACGCCG CCACTCTCTG 
GAGAACAAGG TAAAGAGGCT AGAGACCATG GAGCGTAGAG AAAACAGACT GAAGGATGAC 
ATCCAGACAA AGTCCCAACA GATCCAGCAG ATGGCTGATA AAATTCTGGA GCTGGAGGAG 
AAGCACAGAG AAGCCCAGGT CTCAGCGCAG CATCTGGAGG TGCACCTGAA ACAGAAAGAG 
CAGCACTATG AAGAAAAAAT TAAAGTGTTG GACAATCAGA TAAAGAAAGA CCTAGCAGAC 
AAGGAGACAC TGGAGAACCT GATGCAAAGA CACGAAGAGG AGGCCCATGA GAAAGGCAAA 
ATTCTCAGCG AGCAGAAGGC GATGATCAAT GCTATGGATT CCAAGATCAG ATCCCTGGAA 
CAAAGGATAG TGGAATTGTC GGAAGCCAAT AAACTTGCGG CCAACAGCAG TCTCTTTACC 
CAGAGGAACA TGAAGGCCCA GGAAGAGATG ATTTCAGAAC TCAGGCAACA GAAGTTTTAC 
CTGGAGACGC AAGCTGGGAA ATTGGAGGCC CAGAACCGGA AGCTGGAGGA GCAGCTGGAA 
AAAATAAGCC ACCAAGACCA CAGTGACAAG AACCGTCTGC TGGAGCTGGA GACCAGGCTA 
AGGGAGGTCA GTCTAGAACA TGAAGAGCAG AAACTGGAGC TCAAACGCCA GCTCACAGAG 
CTGCAGCTCT CCCTGCAGGA GCGTGAATCC CAGCTGACAG CCCTGCAGGC AGCACGGGCG 
GCCCTGGAGA GCCAGCTCCG CCAGGCCAAG ACGGAGCTGG AAGAGACGAC AGCAGAAGCA 
GAAGAAGAGA TCCAGGCGCT TACGGCACAT AGAGATGAAA TCCAGCGCAA ATTCGATGCC 
CTTCGTAACA GCTGTACTGT GATCACGGAC TTGGAGGAGC AGCTAAACCA GCTGACGGAG 
GACAATGCCG AGCTCAACAA CCAAAACTTC TACTTGTCTA AACAACTCGA TGAGGCCTCT 
GGTGCCAACG ACGAGATTGT GCAGCTGCGA AGTGAAGTGG ACCATCTCCG CCGCGAGATC 
ACTGAGAGGG AGATGCAGCT CACTAGCCAG AAGCAAACGA TGGAGGCGCT GAAGACCACG 
TGCACAATGC TGGAGGAGCA GGTCATGGAC CTGGAGGCCC TGAACGACGA GCTGCTGGAG 
AAGGAGCGGC AGTGGGAGGC CTGGAGGAGT GTCCTTGGTG ACGAGAAGTC CCAGTTTGAG 
TGTCGGGTTC GAGAGTTGCA GAGGATGCTG GACACCGAAA AGCAGAGCAG GGCGAGGGCC 
GACCAGAGGA TCACTGAGTC GCGCCAGGTG GTCGAGCTGG CAGTAAAGGA GCACAAGGCC 
GAGATCCTGG CCTTGCAGCA GGCTCTCAAG GAGCAGAAGC TGAAGGCCGA GAGCCTCTCC 
GACAAGCTCA ATGATCTGGA GAAGAAGCAC GCCATGCTTG AAATGAATGC CCGAAGTTTA 
CAACAGAAGC TGGAGACAGA ACGAGAGCTC AAACAAAGGC TTCTGGAAGA GCAAGCCAAG 
TTACAGCAGC AGATGGACCT GCAGAAGAAT CATATTTTCC GTCTGACTCA AGGGCTGCAA 
GAAGCTCTGG ATCGGGCTGA TCTGCTGAAG ACAGAAAGGA GTGACCTGGA ATATCAGCTG 
GAAAACATTC AGGTTCTCTA TTCTCATGAG AAGGTGAAGA TGGAAGGCAC TATTTCCCAG 
CAAACCAAAC TCATCGATTT TCTGCAAGCC AAAATGGACC AGCCTGCCAA AAAGAAAAAG 
GTTCCTCTGC AGTACAATGA GCTGAAGGTG GCTCTGGAGA AGGAGAAAGC TCGCTGCGCG 
GAGCTCGAGG AAGCCCTGCA GAAGACCCGC ATCGAGCTCC GCTCCGCCCG GGAGGAAGCT 
GCCCACCGGA AAGCTGCAGA CCACCCACAC CCATCTACGC CGGCTACCGC CAGGCAGCAG 
ATCGCCATGT CCGCCATTGT GCGGTCACCC GAGCACCAGC CCAGCGCCCT GAGCCTGCTC 
GCCCCGCCGT CCAGCCGCAG GAAGGAGTCT TCCACTCCAG AGGAATTCAG CCGGCGTCTT 
AAGGAGCGCA TGCACCACAA CATCCCTCAC CGGTTCAACG TGGGACTGAA CATGCGAGCC 
ACGAAGTGCG CCGTGTGTCT GGATACCGTG CACTTCGGGC GCCAGGCATC CAAGTGTCTC 
GAATGTCAGG TCATGTGCCA CCCCAAGTGC TCCACATGCT TGCCAGCCAC CTGTGGCCTG 
CCAGCCGAAT ACGCCACACA CTTCACCGAG GCCTTCTGCC GCGACAAAAT GAACTCCCCG 
GGTCTGCAGT CCAAGGAGCC CAGCAGCAGC CTGCACCTGG AAGGGTGGAT GAAGGTGCCC 
AGGAATAACA AGCGAGGACA GCAAGGCTGG GACAGGAAGT ACATCGTTCT GGAGGGCTCC 
AAAGTCCTCA TTTATGACAA CGAAGCCAGA GAAGCTGGAC AGAGGCCGGT GGAAGAATTT 
GAGCTGTGCC TTCCCGACGG GGACGTGTCT ATTCATGGCG CCGTTGGTGC TTCTGAACTT 
GCAAACACAG CCAAAGCAGA TGTCCCCTAT GTCCTGAAGA TGGAGTCTCA CCCCCATACC 
ACCTGCTGGC CTGGGAGGAC CCTCTACTTG CTGGCTCCCA GCTTTCCCGA CAAGCAGCGC 
TGGGTCACCG CCTTAGAGTC AGTTGTGGCA GGTGGGAGAG TGTCTAGGGA AAAGGCGGAA 
GCCGACGCTG GCCGTGACTG TACTTCCTAC GAGCCTCTGC CCGCGTGGGC TCAGAAATTG 
CTCGGAAACT CCCTGTTGAA ACTGGAAGGT GATGACCGTC TGGACATGAA CTGCACGCTG 
CCCTTCAGCG ACCAGGTGGT GCTGGTGGGC ACCGAGGAAG GGCTGTATGC GCTGAATGTC 
TTGAAGAACT CCCTCACCCA CGTCCCAGGA ATCGGAGCAG TCTTCCAGAT TTACATCATC 
AAGGACCTGG AGAAGCTCCT CATGATCGCA GGAGAGGAGC GGGCCCTGTG TCTCGTGGAT 
GTGAAGAAGG TGAAGCAGTC CCTCGCGCAG TCTCACCTCC CCGCCCAGCC AGACATCTCG 
CCCAACATCT TTGAAGCGGT CAAGGGCTGC CACTTGTTTG CTGCTGGCAA GATTGAGAGC 
GGGCTCTGCA TCTGTGCAGC CATGCCCAGC AAAGTTGTCA TTCTCCGCTA CAATGAAAAC 
CTCAGCAAGT ACTGCATTCG CAAAGAGATC GAGACCTCGG AGCCCTGCAG CTGTATCCAC 
TTCACCAACT ACAGTATCCT CATCGGAACC AATAAATTCT ACGAAATCGA CATGAAGCAG 
TACACGCTAG AGGAATTCCT GGATAAGAAC GACCATTCCT TGGCGCCTGC CGTGTTTGCC 
TCCTCTTCCA ACAGCTTCCC CGTGTCCATC ATGCAGGTGA ACGGCGCGGG GCAGCGGGAG 
GAGTTCCTGC TCTGTTTCCA CGAATTCGGG GTGTTCGTGG ATTCTTATGG AAGACGCAGC 
CGCACAGATG ATCTCAAGTG GAGTCGCTTA CCTCTGGCCT TTGCCTACAG AGAACCCTAT 
CTGTTTGTGA CTCACTTCAA CTCACTCGAA GTAATTGAGA TCCAGGCCCG CTCCTCTCTG 
GGGACCCCCG CCCGAGCATA TTTGGAAATC CCCAACCCAC GCTACCTGGG CCCTGCAATC 
TCCTCAGGAG CCATCTACCT GGCGTCCTCA TATCAGGATA AGTTAAGGGT CATCTGCTGC 
AAAGGAAACC TGGTGAAGGA GACCGGCACC GACCACCACC GTGGCCCATC CACCTCCCGC 
AGCCCCAACA AGCGAGGCCC GCCGACGTAC AACGAGCACA TCACCAAGCG CGTGGCCTCA 
AGCCCGGCGC CTCCCGAAGG CCCCAGCCAC CCTCGAGAGC CAAGCACACC CCACCGCTAC 
CGCGAGGGGC GGACCGAGCT GCGCAGGGAC AAGTCTCCGG GCCGCCCCCT GGAGCGCGAG 
AAGTCCCCCG GCCGGATGCT GAGCACGCGG AGGGAGCGCT CCCCCGGGCG GCTGTTTGAG 
GACAGCAGCA GGGGCCGGCT GCCCGTGGGG GCCGTGAGGA CCCCGCTGTC CCAGGTCAAC 
AAGGTCTGGG ACCAGTCTTC AGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CIT ( XM_024977737.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024833505.1 Bos taurus citron rho-interacting serine/threonine kinase (CIT), transcript variant X5, mRNA.

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CloneID	OBa224625
Clone ID Related Accession (Same CDS sequence)	XM_024977737.1
Accession Version	XM_024977737.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6162bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	citron Rho-interacting kinase isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGAAGT TCAAGTATGG AGCACGGAAT CTACTGGACG CTGGTACTGT TGAATCCATT 
GCCAGCCGGG CCTCCAGGCT GAATCTTTTC TTCCAGGGGA AACCACCCTC CATGACTCAA 
CAGCAGATGT CTCTTCTTTC CCGAGAAGCG ATATTAGATG CCCTCTTTGT TCTCTTTAAA 
GAATGCAGTC AGCCAGCTCT GATGAAGATT AAGCACGTGA GCAACTTTGT CCAAAAGTAT 
TCCGATATCA TAGCTGAGTT GCAAGAGCTC CAGCCTTCAG TGAAGGACTT TGAAGTGAAA 
AGTGTCGTAG GCTGCGGTCA CTTTGCTGAA GTACAGGTGG TCAGAGAGAA AGCGACTGGG 
GATGTCTATG CCATGAAAGT CATGAAGAAG AAGGCCTTGT TGGCCCAGGA GCAGGTCTCA 
TATTTTGAGG AAGAACAGAA CATATTATCT CAGAGTACAA GTCCTTGGAT CCCCCAGTTG 
CAGTATGCCT TTCAGGACAA AAATAACCTT TATCTGGTCA TGGAATATCA GCCTGGAGGG 
GATTTGCTGT CACTTTTGAA TAGATATGAG GACCAATTAG ATGAAAATAT GATTCAGTTT 
TACCTAGCTG AACTGATTTT GGCTGTCCAC AGCGTTCATC AGATGGGCTA TGTACATCGA 
GACATCAAGC CCGAGAACAT CCTCATTGAC CGAACGGGAC ACATCAAGCT GGTGGATTTT 
GGATCAGCTG CTAAAATGAA CTCAAATAAG ATGGTAAATG CCAAACTCCC GGTTGGTACT 
CCAGATTACA TGGCCCCTGA AGTGTTGACT GTGATGAATG GGGATGGAAA AGGTGCCTAT 
AGCCTAGACT GTGATTGGTG GTCAGTGGGA GTTATTGCTT ATGAGATGGT TTATGGAAGA 
TGCCCATTCA CTGAAGGAAC CTCAGCCAAA ACCTTCAATA ACATCATGAA TTTCCAGCGG 
TTCTTGAAGT TTCCAGATGA CCCCAAAGTT AGCAGTGAAT TGCTTGATCT AATCCAGAGT 
TTGTTGTGTG GCCAGAAAGA GAGACTGAAG TTTGAGGGCC TTTGCTGTCA TCCTTTCTTC 
TCCAAAATTG ACTGGATTAA CATTCGTAAC TCTCCTCCCC CCTTCGTTCC CACCCTCAAG 
TCTGATGATG ACACCTCCAA TTTTGATGAA CCAGAGAAGA ATTCGTGGGT TTCATCCTCT 
CCGTGCCAGC TGAACCCCTC AGGTTTCTCG GGCGAAGAAC TGCCGTTTGT GGGGTTTTCG 
TATAGCAAGG CACTGGGAAT TCTTGGTAGA TCTGAGCCTG TTGTATCAAG TCTGGACTCC 
CCTGCCAAGA CTAGCTCCAT GGAAAAGAAA CTCCTTATCA AAAGCAAAGA GCTGCAGGAC 
TCCCAGGACA AGTGTCACAA GATGGAGCAG GAAATGACCC GGTTACATCG GAGAGTGTCA 
GAGGTGGAGG CTGTGCTTAG TCAGAAGGAG GTGGAGCTGA AGGCCTCTGA GACTCAGAGA 
TCCCTCCTGG AGCAGGACCT TGCTACCTAC ATCACAGAAT GCAGTAGCTT AAAGCGAAGT 
TTGGAGCAAG CACGGATGGA GGTGTCCCAA GAGGACGACA AAGCACTGCA GCTCCTCCAT 
GATATCAGAG AGCAGAGCCG GAAGCTCCAA GAAATCAAAG AGCAGGAGTA CCAGGCTCAA 
GTGGAAGAAA TGAGGTTGAT GATGAATCAG TTGGAAGAGG ACCTGGTTTC GGCGAGAAGG 
CGGAGTGACC TCTATGAATC GGAGCTCAGA GAGTCTCGGC TGGCTGCTGA AGAGTTTAAG 
CGGAAAGCAA CAGAATGTCA GCATAAACTG ATGAAGGCTA AAGATCAAGG GAAGCCTGAA 
GTGGGAGAAT ATGCCAAACT GGAGAAGATC AATGCTGAGC AGCAGCTCAA AATTCAGGAG 
CTCCAAGAGA AGCTAGAAAA GGCTGTGAAA GCTAGCACAG AGGCCACCGA GCTGCTGCAG 
AACATCCGTC AGGCGAAGGA GCGGGCCGAG CGTGAGCTGG AGAAGCTGCA CAACCGTGAG 
GACTCCTCCG AGGGCATCAA GAAGAAGCTG GTGGAGGCCG AGGAACGCCG CCACTCTCTG 
GAGAACAAGG TAAAGAGGCT AGAGACCATG GAGCGTAGAG AAAACAGACT GAAGGATGAC 
ATCCAGACAA AGTCCCAACA GATCCAGCAG ATGGCTGATA AAATTCTGGA GCTGGAGGAG 
AAGCACAGAG AAGCCCAGGT CTCAGCGCAG CATCTGGAGG TGCACCTGAA ACAGAAAGAG 
CAGCACTATG AAGAAAAAAT TAAAGTGTTG GACAATCAGA TAAAGAAAGA CCTAGCAGAC 
AAGGAGACAC TGGAGAACCT GATGCAAAGA CACGAAGAGG AGGCCCATGA GAAAGGCAAA 
ATTCTCAGCG AGCAGAAGGC GATGATCAAT GCTATGGATT CCAAGATCAG ATCCCTGGAA 
CAAAGGATAG TGGAATTGTC GGAAGCCAAT AAACTTGCGG CCAACAGCAG TCTCTTTACC 
CAGAGGAACA TGAAGGCCCA GGAAGAGATG ATTTCAGAAC TCAGGCAACA GAAGTTTTAC 
CTGGAGACGC AAGCTGGGAA ATTGGAGGCC CAGAACCGGA AGCTGGAGGA GCAGCTGGAA 
AAAATAAGCC ACCAAGACCA CAGTGACAAG AACCGTCTGC TGGAGCTGGA GACCAGGCTA 
AGGGAGGTCA GTCTAGAACA TGAAGAGCAG AAACTGGAGC TCAAACGCCA GCTCACAGAG 
CTGCAGCTCT CCCTGCAGGA GCGTGAATCC CAGCTGACAG CCCTGCAGGC AGCACGGGCG 
GCCCTGGAGA GCCAGCTCCG CCAGGCCAAG ACGGAGCTGG AAGAGACGAC AGCAGAAGCA 
GAAGAAGAGA TCCAGGCGCT TACGGCACAT AGAGATGAAA TCCAGCGCAA ATTCGATGCC 
CTTCGTAACA GCTGTACTGT GATCACGGAC TTGGAGGAGC AGCTAAACCA GCTGACGGAG 
GACAATGCCG AGCTCAACAA CCAAAACTTC TACTTGTCTA AACAACTCGA TGAGGCCTCT 
GGTGCCAACG ACGAGATTGT GCAGCTGCGA AGTGAAGTGG ACCATCTCCG CCGCGAGATC 
ACTGAGAGGG AGATGCAGCT CACTAGCCAG AAGCAAACGA TGGAGGCGCT GAAGACCACG 
TGCACAATGC TGGAGGAGCA GGTCATGGAC CTGGAGGCCC TGAACGACGA GCTGCTGGAG 
AAGGAGCGGC AGTGGGAGGC CTGGAGGAGT GTCCTTGGTG ACGAGAAGTC CCAGTTTGAG 
TGTCGGGTTC GAGAGTTGCA GAGGATGCTG GACACCGAAA AGCAGAGCAG GGCGAGGGCC 
GACCAGAGGA TCACTGAGTC GCGCCAGGTG GTCGAGCTGG CAGTAAAGGA GCACAAGGCC 
GAGATCCTGG CCTTGCAGCA GGCTCTCAAG GAGCAGAAGC TGAAGGCCGA GAGCCTCTCC 
GACAAGCTCA ATGATCTGGA GAAGAAGCAC GCCATGCTTG AAATGAATGC CCGAAGTTTA 
CAACAGAAGC TGGAGACAGA ACGAGAGCTC AAACAAAGGC TTCTGGAAGA GCAAGCCAAG 
TTACAGCAGC AGATGGACCT GCAGAAGAAT CATATTTTCC GTCTGACTCA AGGGCTGCAA 
GAAGCTCTGG ATCGGGCTGA TCTGCTGAAG ACAGAAAGGA GTGACCTGGA ATATCAGCTG 
GAAAACATTC AGGTTCTCTA TTCTCATGAG AAGGTGAAGA TGGAAGGCAC TATTTCCCAG 
CAAACCAAAC TCATCGATTT TCTGCAAGCC AAAATGGACC AGCCTGCCAA AAAGAAAAAG 
GTTCCTCTGC AGTACAATGA GCTGAAGGTG GCTCTGGAGA AGGAGAAAGC TCGCTGCGCG 
GAGCTCGAGG AAGCCCTGCA GAAGACCCGC ATCGAGCTCC GCTCCGCCCG GGAGGAAGCT 
GCCCACCGGA AAGCTGCAGA CCACCCACAC CCATCTACGC CGGCTACCGC CAGGCAGCAG 
ATCGCCATGT CCGCCATTGT GCGGTCACCC GAGCACCAGC CCAGCGCCCT GAGCCTGCTC 
GCCCCGCCGT CCAGCCGCAG GAAGGAGTCT TCCACTCCAG AGGAATTCAG CCGGCGTCTT 
AAGGAGCGCA TGCACCACAA CATCCCTCAC CGGTTCAACG TGGGACTGAA CATGCGAGCC 
ACGAAGTGCG CCGTGTGTCT GGATACCGTG CACTTCGGGC GCCAGGCATC CAAGTGTCTC 
GAATGTCAGG TCATGTGCCA CCCCAAGTGC TCCACATGCT TGCCAGCCAC CTGTGGCCTG 
CCAGCCGAAT ACGCCACACA CTTCACCGAG GCCTTCTGCC GCGACAAAAT GAACTCCCCG 
GGTCTGCAGT CCAAGGAGCC CAGCAGCAGC CTGCACCTGG AAGGGTGGAT GAAGGTGCCC 
AGGAATAACA AGCGAGGACA GCAAGGCTGG GACAGGAAGT ACATCGTTCT GGAGGGCTCC 
AAAGTCCTCA TTTATGACAA CGAAGCCAGA GAAGCTGGAC AGAGGCCGGT GGAAGAATTT 
GAGCTGTGCC TTCCCGACGG GGACGTGTCT ATTCATGGCG CCGTTGGTGC TTCTGAACTT 
GCAAACACAG CCAAAGCAGA TGTCCCCTAT GTCCTGAAGA TGGAGTCTCA CCCCCATACC 
ACCTGCTGGC CTGGGAGGAC CCTCTACTTG CTGGCTCCCA GCTTTCCCGA CAAGCAGCGC 
TGGGTCACCG CCTTAGAGTC AGTTGTGGCA GGTGGGAGAG TGTCTAGGGA AAAGGCGGAA 
GCCGACGCTA AATTGCTCGG AAACTCCCTG TTGAAACTGG AAGGTGATGA CCGTCTGGAC 
ATGAACTGCA CGCTGCCCTT CAGCGACCAG GTGGTGCTGG TGGGCACCGA GGAAGGGCTG 
TATGCGCTGA ATGTCTTGAA GAACTCCCTC ACCCACGTCC CAGGAATCGG AGCAGTCTTC 
CAGATTTACA TCATCAAGGA CCTGGAGAAG CTCCTCATGA TCGCAGGAGA GGAGCGGGCC 
CTGTGTCTCG TGGATGTGAA GAAGGTGAAG CAGTCCCTCG CGCAGTCTCA CCTCCCCGCC 
CAGCCAGACA TCTCGCCCAA CATCTTTGAA GCGGTCAAGG GCTGCCACTT GTTTGCTGCT 
GGCAAGATTG AGAGCGGGCT CTGCATCTGT GCAGCCATGC CCAGCAAAGT TGTCATTCTC 
CGCTACAATG AAAACCTCAG CAAGTACTGC ATTCGCAAAG AGATCGAGAC CTCGGAGCCC 
TGCAGCTGTA TCCACTTCAC CAACTACAGT ATCCTCATCG GAACCAATAA ATTCTACGAA 
ATCGACATGA AGCAGTACAC GCTAGAGGAA TTCCTGGATA AGAACGACCA TTCCTTGGCG 
CCTGCCGTGT TTGCCTCCTC TTCCAACAGC TTCCCCGTGT CCATCATGCA GGTGAACGGC 
GCGGGGCAGC GGGAGGAGTT CCTGCTCTGT TTCCACGAAT TCGGGGTGTT CGTGGATTCT 
TATGGAAGAC GCAGCCGCAC AGATGATCTC AAGTGGAGTC GCTTACCTCT GGCCTTTGCC 
TACAGAGAAC CCTATCTGTT TGTGACTCAC TTCAACTCAC TCGAAGTAAT TGAGATCCAG 
GCCCGCTCCT CTCTGGGGAC CCCCGCCCGA GCATATTTGG AAATCCCCAA CCCACGCTAC 
CTGGGCCCTG CAATCTCCTC AGGAGCCATC TACCTGGCGT CCTCATATCA GGATAAGTTA 
AGGGTCATCT GCTGCAAAGG AAACCTGGTG AAGGAGACCG GCACCGACCA CCACCGTGGC 
CCATCCACCT CCCGCAGCCC CAACAAGCGA GGCCCGCCGA CGTACAACGA GCACATCACC 
AAGCGCGTGG CCTCAAGCCC GGCGCCTCCC GAAGGCCCCA GCCACCCTCG AGAGCCAAGC 
ACACCCCACC GCTACCGCGA GGGGCGGACC GAGCTGCGCA GGGACAAGTC TCCGGGCCGC 
CCCCTGGAGC GCGAGAAGTC CCCCGGCCGG ATGCTGAGCA CGCGGAGGGA GCGCTCCCCC 
GGGCGGCTGT TTGAGGACAG CAGCAGGGGC CGGCTGCCCG TGGGGGCCGT GAGGACCCCG 
CTGTCCCAGG TCAACAAGGT CTGGGACCAG TCTTCAGTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024833505.1
CDS	113..6274
Translation	MLKFKYGARNLLDAGTVESIASRASRLNLFFQGKPPSMTQQQMSLLSREAILDALFVLFKECSQPALMKIKHVSNFVQKYSDIIAELQELQPSVKDFEVKSVVGCGHFAEVQVVREKATGDVYAMKVMKKKALLAQEQVSYFEEEQNILSQSTSPWIPQLQYAFQDKNNLYLVMEYQPGGDLLSLLNRYEDQLDENMIQFYLAELILAVHSVHQMGYVHRDIKPENILIDRTGHIKLVDFGSAAKMNSNKMVNAKLPVGTPDYMAPEVLTVMNGDGKGAYSLDCDWWSVGVIAYEMVYGRCPFTEGTSAKTFNNIMNFQRFLKFPDDPKVSSELLDLIQSLLCGQKERLKFEGLCCHPFFSKIDWINIRNSPPPFVPTLKSDDDTSNFDEPEKNSWVSSSPCQLNPSGFSGEELPFVGFSYSKALGILGRSEPVVSSLDSPAKTSSMEKKLLIKSKELQDSQDKCHKMEQEMTRLHRRVSEVEAVLSQKEVELKASETQRSLLEQDLATYITECSSLKRSLEQARMEVSQEDDKALQLLHDIREQSRKLQEIKEQEYQAQVEEMRLMMNQLEEDLVSARRRSDLYESELRESRLAAEEFKRKATECQHKLMKAKDQGKPEVGEYAKLEKINAEQQLKIQELQEKLEKAVKASTEATELLQNIRQAKERAERELEKLHNREDSSEGIKKKLVEAEERRHSLENKVKRLETMERRENRLKDDIQTKSQQIQQMADKILELEEKHREAQVSAQHLEVHLKQKEQHYEEKIKVLDNQIKKDLADKETLENLMQRHEEEAHEKGKILSEQKAMINAMDSKIRSLEQRIVELSEANKLAANSSLFTQRNMKAQEEMISELRQQKFYLETQAGKLEAQNRKLEEQLEKISHQDHSDKNRLLELETRLREVSLEHEEQKLELKRQLTELQLSLQERESQLTALQAARAALESQLRQAKTELEETTAEAEEEIQALTAHRDEIQRKFDALRNSCTVITDLEEQLNQLTEDNAELNNQNFYLSKQLDEASGANDEIVQLRSEVDHLRREITEREMQLTSQKQTMEALKTTCTMLEEQVMDLEALNDELLEKERQWEAWRSVLGDEKSQFECRVRELQRMLDTEKQSRARADQRITESRQVVELAVKEHKAEILALQQALKEQKLKAESLSDKLNDLEKKHAMLEMNARSLQQKLETERELKQRLLEEQAKLQQQMDLQKNHIFRLTQGLQEALDRADLLKTERSDLEYQLENIQVLYSHEKVKMEGTISQQTKLIDFLQAKMDQPAKKKKVPLQYNELKVALEKEKARCAELEEALQKTRIELRSAREEAAHRKAADHPHPSTPATARQQIAMSAIVRSPEHQPSALSLLAPPSSRRKESSTPEEFSRRLKERMHHNIPHRFNVGLNMRATKCAVCLDTVHFGRQASKCLECQVMCHPKCSTCLPATCGLPAEYATHFTEAFCRDKMNSPGLQSKEPSSSLHLEGWMKVPRNNKRGQQGWDRKYIVLEGSKVLIYDNEAREAGQRPVEEFELCLPDGDVSIHGAVGASELANTAKADVPYVLKMESHPHTTCWPGRTLYLLAPSFPDKQRWVTALESVVAGGRVSREKAEADAKLLGNSLLKLEGDDRLDMNCTLPFSDQVVLVGTEEGLYALNVLKNSLTHVPGIGAVFQIYIIKDLEKLLMIAGEERALCLVDVKKVKQSLAQSHLPAQPDISPNIFEAVKGCHLFAAGKIESGLCICAAMPSKVVILRYNENLSKYCIRKEIETSEPCSCIHFTNYSILIGTNKFYEIDMKQYTLEEFLDKNDHSLAPAVFASSSNSFPVSIMQVNGAGQREEFLLCFHEFGVFVDSYGRRSRTDDLKWSRLPLAFAYREPYLFVTHFNSLEVIEIQARSSLGTPARAYLEIPNPRYLGPAISSGAIYLASSYQDKLRVICCKGNLVKETGTDHHRGPSTSRSPNKRGPPTYNEHITKRVASSPAPPEGPSHPREPSTPHRYREGRTELRRDKSPGRPLEREKSPGRMLSTRRERSPGRLFEDSSRGRLPVGAVRTPLSQVNKVWDQSSV

Target ORF information:

RefSeq Version	XM_024977737.1
Organism	Bos taurus(cattle)
Definition	Bos taurus citron rho-interacting serine/threonine kinase (CIT), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024977737.1

ORF Insert Sequence:

ATGTTGAAGT TCAAGTATGG AGCACGGAAT CTACTGGACG CTGGTACTGT TGAATCCATT 
GCCAGCCGGG CCTCCAGGCT GAATCTTTTC TTCCAGGGGA AACCACCCTC CATGACTCAA 
CAGCAGATGT CTCTTCTTTC CCGAGAAGCG ATATTAGATG CCCTCTTTGT TCTCTTTAAA 
GAATGCAGTC AGCCAGCTCT GATGAAGATT AAGCACGTGA GCAACTTTGT CCAAAAGTAT 
TCCGATATCA TAGCTGAGTT GCAAGAGCTC CAGCCTTCAG TGAAGGACTT TGAAGTGAAA 
AGTGTCGTAG GCTGCGGTCA CTTTGCTGAA GTACAGGTGG TCAGAGAGAA AGCGACTGGG 
GATGTCTATG CCATGAAAGT CATGAAGAAG AAGGCCTTGT TGGCCCAGGA GCAGGTCTCA 
TATTTTGAGG AAGAACAGAA CATATTATCT CAGAGTACAA GTCCTTGGAT CCCCCAGTTG 
CAGTATGCCT TTCAGGACAA AAATAACCTT TATCTGGTCA TGGAATATCA GCCTGGAGGG 
GATTTGCTGT CACTTTTGAA TAGATATGAG GACCAATTAG ATGAAAATAT GATTCAGTTT 
TACCTAGCTG AACTGATTTT GGCTGTCCAC AGCGTTCATC AGATGGGCTA TGTACATCGA 
GACATCAAGC CCGAGAACAT CCTCATTGAC CGAACGGGAC ACATCAAGCT GGTGGATTTT 
GGATCAGCTG CTAAAATGAA CTCAAATAAG ATGGTAAATG CCAAACTCCC GGTTGGTACT 
CCAGATTACA TGGCCCCTGA AGTGTTGACT GTGATGAATG GGGATGGAAA AGGTGCCTAT 
AGCCTAGACT GTGATTGGTG GTCAGTGGGA GTTATTGCTT ATGAGATGGT TTATGGAAGA 
TGCCCATTCA CTGAAGGAAC CTCAGCCAAA ACCTTCAATA ACATCATGAA TTTCCAGCGG 
TTCTTGAAGT TTCCAGATGA CCCCAAAGTT AGCAGTGAAT TGCTTGATCT AATCCAGAGT 
TTGTTGTGTG GCCAGAAAGA GAGACTGAAG TTTGAGGGCC TTTGCTGTCA TCCTTTCTTC 
TCCAAAATTG ACTGGATTAA CATTCGTAAC TCTCCTCCCC CCTTCGTTCC CACCCTCAAG 
TCTGATGATG ACACCTCCAA TTTTGATGAA CCAGAGAAGA ATTCGTGGGT TTCATCCTCT 
CCGTGCCAGC TGAACCCCTC AGGTTTCTCG GGCGAAGAAC TGCCGTTTGT GGGGTTTTCG 
TATAGCAAGG CACTGGGAAT TCTTGGTAGA TCTGAGCCTG TTGTATCAAG TCTGGACTCC 
CCTGCCAAGA CTAGCTCCAT GGAAAAGAAA CTCCTTATCA AAAGCAAAGA GCTGCAGGAC 
TCCCAGGACA AGTGTCACAA GATGGAGCAG GAAATGACCC GGTTACATCG GAGAGTGTCA 
GAGGTGGAGG CTGTGCTTAG TCAGAAGGAG GTGGAGCTGA AGGCCTCTGA GACTCAGAGA 
TCCCTCCTGG AGCAGGACCT TGCTACCTAC ATCACAGAAT GCAGTAGCTT AAAGCGAAGT 
TTGGAGCAAG CACGGATGGA GGTGTCCCAA GAGGACGACA AAGCACTGCA GCTCCTCCAT 
GATATCAGAG AGCAGAGCCG GAAGCTCCAA GAAATCAAAG AGCAGGAGTA CCAGGCTCAA 
GTGGAAGAAA TGAGGTTGAT GATGAATCAG TTGGAAGAGG ACCTGGTTTC GGCGAGAAGG 
CGGAGTGACC TCTATGAATC GGAGCTCAGA GAGTCTCGGC TGGCTGCTGA AGAGTTTAAG 
CGGAAAGCAA CAGAATGTCA GCATAAACTG ATGAAGGCTA AAGATCAAGG GAAGCCTGAA 
GTGGGAGAAT ATGCCAAACT GGAGAAGATC AATGCTGAGC AGCAGCTCAA AATTCAGGAG 
CTCCAAGAGA AGCTAGAAAA GGCTGTGAAA GCTAGCACAG AGGCCACCGA GCTGCTGCAG 
AACATCCGTC AGGCGAAGGA GCGGGCCGAG CGTGAGCTGG AGAAGCTGCA CAACCGTGAG 
GACTCCTCCG AGGGCATCAA GAAGAAGCTG GTGGAGGCCG AGGAACGCCG CCACTCTCTG 
GAGAACAAGG TAAAGAGGCT AGAGACCATG GAGCGTAGAG AAAACAGACT GAAGGATGAC 
ATCCAGACAA AGTCCCAACA GATCCAGCAG ATGGCTGATA AAATTCTGGA GCTGGAGGAG 
AAGCACAGAG AAGCCCAGGT CTCAGCGCAG CATCTGGAGG TGCACCTGAA ACAGAAAGAG 
CAGCACTATG AAGAAAAAAT TAAAGTGTTG GACAATCAGA TAAAGAAAGA CCTAGCAGAC 
AAGGAGACAC TGGAGAACCT GATGCAAAGA CACGAAGAGG AGGCCCATGA GAAAGGCAAA 
ATTCTCAGCG AGCAGAAGGC GATGATCAAT GCTATGGATT CCAAGATCAG ATCCCTGGAA 
CAAAGGATAG TGGAATTGTC GGAAGCCAAT AAACTTGCGG CCAACAGCAG TCTCTTTACC 
CAGAGGAACA TGAAGGCCCA GGAAGAGATG ATTTCAGAAC TCAGGCAACA GAAGTTTTAC 
CTGGAGACGC AAGCTGGGAA ATTGGAGGCC CAGAACCGGA AGCTGGAGGA GCAGCTGGAA 
AAAATAAGCC ACCAAGACCA CAGTGACAAG AACCGTCTGC TGGAGCTGGA GACCAGGCTA 
AGGGAGGTCA GTCTAGAACA TGAAGAGCAG AAACTGGAGC TCAAACGCCA GCTCACAGAG 
CTGCAGCTCT CCCTGCAGGA GCGTGAATCC CAGCTGACAG CCCTGCAGGC AGCACGGGCG 
GCCCTGGAGA GCCAGCTCCG CCAGGCCAAG ACGGAGCTGG AAGAGACGAC AGCAGAAGCA 
GAAGAAGAGA TCCAGGCGCT TACGGCACAT AGAGATGAAA TCCAGCGCAA ATTCGATGCC 
CTTCGTAACA GCTGTACTGT GATCACGGAC TTGGAGGAGC AGCTAAACCA GCTGACGGAG 
GACAATGCCG AGCTCAACAA CCAAAACTTC TACTTGTCTA AACAACTCGA TGAGGCCTCT 
GGTGCCAACG ACGAGATTGT GCAGCTGCGA AGTGAAGTGG ACCATCTCCG CCGCGAGATC 
ACTGAGAGGG AGATGCAGCT CACTAGCCAG AAGCAAACGA TGGAGGCGCT GAAGACCACG 
TGCACAATGC TGGAGGAGCA GGTCATGGAC CTGGAGGCCC TGAACGACGA GCTGCTGGAG 
AAGGAGCGGC AGTGGGAGGC CTGGAGGAGT GTCCTTGGTG ACGAGAAGTC CCAGTTTGAG 
TGTCGGGTTC GAGAGTTGCA GAGGATGCTG GACACCGAAA AGCAGAGCAG GGCGAGGGCC 
GACCAGAGGA TCACTGAGTC GCGCCAGGTG GTCGAGCTGG CAGTAAAGGA GCACAAGGCC 
GAGATCCTGG CCTTGCAGCA GGCTCTCAAG GAGCAGAAGC TGAAGGCCGA GAGCCTCTCC 
GACAAGCTCA ATGATCTGGA GAAGAAGCAC GCCATGCTTG AAATGAATGC CCGAAGTTTA 
CAACAGAAGC TGGAGACAGA ACGAGAGCTC AAACAAAGGC TTCTGGAAGA GCAAGCCAAG 
TTACAGCAGC AGATGGACCT GCAGAAGAAT CATATTTTCC GTCTGACTCA AGGGCTGCAA 
GAAGCTCTGG ATCGGGCTGA TCTGCTGAAG ACAGAAAGGA GTGACCTGGA ATATCAGCTG 
GAAAACATTC AGGTTCTCTA TTCTCATGAG AAGGTGAAGA TGGAAGGCAC TATTTCCCAG 
CAAACCAAAC TCATCGATTT TCTGCAAGCC AAAATGGACC AGCCTGCCAA AAAGAAAAAG 
GTTCCTCTGC AGTACAATGA GCTGAAGGTG GCTCTGGAGA AGGAGAAAGC TCGCTGCGCG 
GAGCTCGAGG AAGCCCTGCA GAAGACCCGC ATCGAGCTCC GCTCCGCCCG GGAGGAAGCT 
GCCCACCGGA AAGCTGCAGA CCACCCACAC CCATCTACGC CGGCTACCGC CAGGCAGCAG 
ATCGCCATGT CCGCCATTGT GCGGTCACCC GAGCACCAGC CCAGCGCCCT GAGCCTGCTC 
GCCCCGCCGT CCAGCCGCAG GAAGGAGTCT TCCACTCCAG AGGAATTCAG CCGGCGTCTT 
AAGGAGCGCA TGCACCACAA CATCCCTCAC CGGTTCAACG TGGGACTGAA CATGCGAGCC 
ACGAAGTGCG CCGTGTGTCT GGATACCGTG CACTTCGGGC GCCAGGCATC CAAGTGTCTC 
GAATGTCAGG TCATGTGCCA CCCCAAGTGC TCCACATGCT TGCCAGCCAC CTGTGGCCTG 
CCAGCCGAAT ACGCCACACA CTTCACCGAG GCCTTCTGCC GCGACAAAAT GAACTCCCCG 
GGTCTGCAGT CCAAGGAGCC CAGCAGCAGC CTGCACCTGG AAGGGTGGAT GAAGGTGCCC 
AGGAATAACA AGCGAGGACA GCAAGGCTGG GACAGGAAGT ACATCGTTCT GGAGGGCTCC 
AAAGTCCTCA TTTATGACAA CGAAGCCAGA GAAGCTGGAC AGAGGCCGGT GGAAGAATTT 
GAGCTGTGCC TTCCCGACGG GGACGTGTCT ATTCATGGCG CCGTTGGTGC TTCTGAACTT 
GCAAACACAG CCAAAGCAGA TGTCCCCTAT GTCCTGAAGA TGGAGTCTCA CCCCCATACC 
ACCTGCTGGC CTGGGAGGAC CCTCTACTTG CTGGCTCCCA GCTTTCCCGA CAAGCAGCGC 
TGGGTCACCG CCTTAGAGTC AGTTGTGGCA GGTGGGAGAG TGTCTAGGGA AAAGGCGGAA 
GCCGACGCTA AATTGCTCGG AAACTCCCTG TTGAAACTGG AAGGTGATGA CCGTCTGGAC 
ATGAACTGCA CGCTGCCCTT CAGCGACCAG GTGGTGCTGG TGGGCACCGA GGAAGGGCTG 
TATGCGCTGA ATGTCTTGAA GAACTCCCTC ACCCACGTCC CAGGAATCGG AGCAGTCTTC 
CAGATTTACA TCATCAAGGA CCTGGAGAAG CTCCTCATGA TCGCAGGAGA GGAGCGGGCC 
CTGTGTCTCG TGGATGTGAA GAAGGTGAAG CAGTCCCTCG CGCAGTCTCA CCTCCCCGCC 
CAGCCAGACA TCTCGCCCAA CATCTTTGAA GCGGTCAAGG GCTGCCACTT GTTTGCTGCT 
GGCAAGATTG AGAGCGGGCT CTGCATCTGT GCAGCCATGC CCAGCAAAGT TGTCATTCTC 
CGCTACAATG AAAACCTCAG CAAGTACTGC ATTCGCAAAG AGATCGAGAC CTCGGAGCCC 
TGCAGCTGTA TCCACTTCAC CAACTACAGT ATCCTCATCG GAACCAATAA ATTCTACGAA 
ATCGACATGA AGCAGTACAC GCTAGAGGAA TTCCTGGATA AGAACGACCA TTCCTTGGCG 
CCTGCCGTGT TTGCCTCCTC TTCCAACAGC TTCCCCGTGT CCATCATGCA GGTGAACGGC 
GCGGGGCAGC GGGAGGAGTT CCTGCTCTGT TTCCACGAAT TCGGGGTGTT CGTGGATTCT 
TATGGAAGAC GCAGCCGCAC AGATGATCTC AAGTGGAGTC GCTTACCTCT GGCCTTTGCC 
TACAGAGAAC CCTATCTGTT TGTGACTCAC TTCAACTCAC TCGAAGTAAT TGAGATCCAG 
GCCCGCTCCT CTCTGGGGAC CCCCGCCCGA GCATATTTGG AAATCCCCAA CCCACGCTAC 
CTGGGCCCTG CAATCTCCTC AGGAGCCATC TACCTGGCGT CCTCATATCA GGATAAGTTA 
AGGGTCATCT GCTGCAAAGG AAACCTGGTG AAGGAGACCG GCACCGACCA CCACCGTGGC 
CCATCCACCT CCCGCAGCCC CAACAAGCGA GGCCCGCCGA CGTACAACGA GCACATCACC 
AAGCGCGTGG CCTCAAGCCC GGCGCCTCCC GAAGGCCCCA GCCACCCTCG AGAGCCAAGC 
ACACCCCACC GCTACCGCGA GGGGCGGACC GAGCTGCGCA GGGACAAGTC TCCGGGCCGC 
CCCCTGGAGC GCGAGAAGTC CCCCGGCCGG ATGCTGAGCA CGCGGAGGGA GCGCTCCCCC 
GGGCGGCTGT TTGAGGACAG CAGCAGGGGC CGGCTGCCCG TGGGGGCCGT GAGGACCCCG 
CTGTCCCAGG TCAACAAGGT CTGGGACCAG TCTTCAGTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CIT ( XM_024977738.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024833506.1 Bos taurus citron rho-interacting serine/threonine kinase (CIT), transcript variant X6, mRNA.

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CloneID	OBa224626
Clone ID Related Accession (Same CDS sequence)	XM_024977738.1
Accession Version	XM_024977738.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6054bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	citron Rho-interacting kinase isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGAAGT TCAAGTATGG AGCACGGAAT CTACTGGACG CTGGTACTGT TGAATCCATT 
GCCAGCCGGG CCTCCAGGCT GAATCTTTTC TTCCAGGGGA AACCACCCTC CATGACTCAA 
CAGCAGATGT CTCTTCTTTC CCGAGAAGCG ATATTAGATG CCCTCTTTGT TCTCTTTAAA 
GAATGCAGTC AGCCAGCTCT GATGAAGATT AAGCACGTGA GCAACTTTGT CCAAAAGTAT 
TCCGATATCA TAGCTGAGTT GCAAGAGCTC CAGCCTTCAG TGAAGGACTT TGAAGTGAAA 
AGTGTCGTAG GCTGCGGTCA CTTTGCTGAA GTACAGGTGG TCAGAGAGAA AGCGACTGGG 
GATGTCTATG CCATGAAAGT CATGAAGAAG AAGGCCTTGT TGGCCCAGGA GCAGGTCTCA 
TATTTTGAGG AAGAACAGAA CATATTATCT CAGAGTACAA GTCCTTGGAT CCCCCAGTTG 
CAGTATGCCT TTCAGGACAA AAATAACCTT TATCTGGTCA TGGAATATCA GCCTGGAGGG 
GATTTGCTGT CACTTTTGAA TAGATATGAG GACCAATTAG ATGAAAATAT GATTCAGTTT 
TACCTAGCTG AACTGATTTT GGCTGTCCAC AGCGTTCATC AGATGGGCTA TGTACATCGA 
GACATCAAGC CCGAGAACAT CCTCATTGAC CGAACGGGAC ACATCAAGCT GGTGGATTTT 
GGATCAGCTG CTAAAATGAA CTCAAATAAG ATGGTAAATG CCAAACTCCC GGTTGGTACT 
CCAGATTACA TGGCCCCTGA AGTGTTGACT GTGATGAATG GGGATGGAAA AGGTGCCTAT 
AGCCTAGACT GTGATTGGTG GTCAGTGGGA GTTATTGCTT ATGAGATGGT TTATGGAAGA 
TGCCCATTCA CTGAAGGAAC CTCAGCCAAA ACCTTCAATA ACATCATGAA TTTCCAGCGG 
TTCTTGAAGT TTCCAGATGA CCCCAAAGTT AGCAGTGAAT TGCTTGATCT AATCCAGAGT 
TTGTTGTGTG GCCAGAAAGA GAGACTGAAG TTTGAGGGCC TTTGCTGTCA TCCTTTCTTC 
TCCAAAATTG ACTGGATTAA CATTCGTAAC TCTCCTCCCC CCTTCGTTCC CACCCTCAAG 
TCTGATGATG ACACCTCCAA TTTTGATGAA CCAGAGAAGA ATTCGTGGGT TTCATCCTCT 
CCGTGCCAGC TGAACCCCTC AGGTTTCTCG GGCGAAGAAC TGCCGTTTGT GGGGTTTTCG 
TATAGCAAGG CACTGGGAAT TCTTGGTAGA TCTGAGCCTG TTGTATCAAG TCTGGACTCC 
CCTGCCAAGA CTAGCTCCAT GGAAAAGAAA CTCCTTATCA AAAGCAAAGA GCTGCAGGAC 
TCCCAGGACA AGTGTCACAA GATGGAGCAG GAAATGACCC GGTTACATCG GAGAGTGTCA 
GAGGTGGAGG CTGTGCTTAG TCAGAAGGAG GTGGAGCTGA AGGCCTCTGA GACTCAGAGA 
TCCCTCCTGG AGCAGGACCT TGCTACCTAC ATCACAGAAT GCAGTAGCTT AAAGCGAAGT 
TTGGAGCAAG CACGGATGGA GGTGTCCCAA GAGGACGACA AAGCACTGCA GCTCCTCCAT 
GATATCAGAG AGCAGAGCCG GAAGCTCCAA GAAATCAAAG AGCAGGAGTA CCAGGCTCAA 
GTGGAAGAAA TGAGGTTGAT GATGAATCAG TTGGAAGAGG ACCTGGTTTC GGCGAGAAGG 
CGGAGTGACC TCTATGAATC GGAGCTCAGA GAGTCTCGGC TGGCTGCTGA AGAGTTTAAG 
CGGAAAGCAA CAGAATGTCA GCATAAACTG ATGAAGGCTA AAGATCAAGG GAAGCCTGAA 
GTGGGAGAAT ATGCCAAACT GGAGAAGATC AATGCTGAGC AGCAGCTCAA AATTCAGGAG 
CTCCAAGAGA AGCTAGAAAA GGCTGTGAAA GCTAGCACAG AGGCCACCGA GCTGCTGCAG 
AACATCCGTC AGGCGAAGGA GCGGGCCGAG CGTGAGCTGG AGAAGCTGCA CAACCGTGAG 
GACTCCTCCG AGGGCATCAA GAAGAAGCTG GTGGAGGCCG AGGAACGCCG CCACTCTCTG 
GAGAACAAGG TAAAGAGGCT AGAGACCATG GAGCGTAGAG AAAACAGACT GAAGGATGAC 
ATCCAGACAA AGTCCCAACA GATCCAGCAG ATGGCTGATA AAATTCTGGA GCTGGAGGAG 
AAGCACAGAG AAGCCCAGGT CTCAGCGCAG CATCTGGAGG TGCACCTGAA ACAGAAAGAG 
CAGCACTATG AAGAAAAAAT TAAAGTGTTG GACAATCAGA TAAAGAAAGA CCTAGCAGAC 
AAGGAGACAC TGGAGAACCT GATGCAAAGA CACGAAGAGG AGGCCCATGA GAAAGGCAAA 
ATTCTCAGCG AGCAGAAGGC GATGATCAAT GCTATGGATT CCAAGATCAG ATCCCTGGAA 
CAAAGGATAG TGGAATTGTC GGAAGCCAAT AAACTTGCGG CCAACAGCAG TCTCTTTACC 
CAGAGGAACA TGAAGGCCCA GGAAGAGATG ATTTCAGAAC TCAGGCAACA GAAGTTTTAC 
CTGGAGACGC AAGCTGGGAA ATTGGAGGCC CAGAACCGGA AGCTGGAGGA GCAGCTGGAA 
AAAATAAGCC ACCAAGACCA CAGTGACAAG AACCGTCTGC TGGAGCTGGA GACCAGGCTA 
AGGGAGGCAC ATAGAGATGA AATCCAGCGC AAATTCGATG CCCTTCGTAA CAGCTGTACT 
GTGATCACGG ACTTGGAGGA GCAGCTAAAC CAGCTGACGG AGGACAATGC CGAGCTCAAC 
AACCAAAACT TCTACTTGTC TAAACAACTC GATGAGGCCT CTGGTGCCAA CGACGAGATT 
GTGCAGCTGC GAAGTGAAGT GGACCATCTC CGCCGCGAGA TCACTGAGAG GGAGATGCAG 
CTCACTAGCC AGAAGCAAAC GATGGAGGCG CTGAAGACCA CGTGCACAAT GCTGGAGGAG 
CAGGTCATGG ACCTGGAGGC CCTGAACGAC GAGCTGCTGG AGAAGGAGCG GCAGTGGGAG 
GCCTGGAGGA GTGTCCTTGG TGACGAGAAG TCCCAGTTTG AGTGTCGGGT TCGAGAGTTG 
CAGAGGATGC TGGACACCGA AAAGCAGAGC AGGGCGAGGG CCGACCAGAG GATCACTGAG 
TCGCGCCAGG TGGTCGAGCT GGCAGTAAAG GAGCACAAGG CCGAGATCCT GGCCTTGCAG 
CAGGCTCTCA AGGAGCAGAA GCTGAAGGCC GAGAGCCTCT CCGACAAGCT CAATGATCTG 
GAGAAGAAGC ACGCCATGCT TGAAATGAAT GCCCGAAGTT TACAACAGAA GCTGGAGACA 
GAACGAGAGC TCAAACAAAG GCTTCTGGAA GAGCAAGCCA AGTTACAGCA GCAGATGGAC 
CTGCAGAAGA ATCATATTTT CCGTCTGACT CAAGGGCTGC AAGAAGCTCT GGATCGGGCT 
GATCTGCTGA AGACAGAAAG GAGTGACCTG GAATATCAGC TGGAAAACAT TCAGGTTCTC 
TATTCTCATG AGAAGGTGAA GATGGAAGGC ACTATTTCCC AGCAAACCAA ACTCATCGAT 
TTTCTGCAAG CCAAAATGGA CCAGCCTGCC AAAAAGAAAA AGGGTTTATT TAGTCGACGG 
AAAGAGGACC CTGCTTTGCC CACACAGGTT CCTCTGCAGT ACAATGAGCT GAAGGTGGCT 
CTGGAGAAGG AGAAAGCTCG CTGCGCGGAG CTCGAGGAAG CCCTGCAGAA GACCCGCATC 
GAGCTCCGCT CCGCCCGGGA GGAAGCTGCC CACCGGAAAG CTGCAGACCA CCCACACCCA 
TCTACGCCGG CTACCGCCAG GCAGCAGATC GCCATGTCCG CCATTGTGCG GTCACCCGAG 
CACCAGCCCA GCGCCCTGAG CCTGCTCGCC CCGCCGTCCA GCCGCAGGAA GGAGTCTTCC 
ACTCCAGAGG AATTCAGCCG GCGTCTTAAG GAGCGCATGC ACCACAACAT CCCTCACCGG 
TTCAACGTGG GACTGAACAT GCGAGCCACG AAGTGCGCCG TGTGTCTGGA TACCGTGCAC 
TTCGGGCGCC AGGCATCCAA GTGTCTCGAA TGTCAGGTCA TGTGCCACCC CAAGTGCTCC 
ACATGCTTGC CAGCCACCTG TGGCCTGCCA GCCGAATACG CCACACACTT CACCGAGGCC 
TTCTGCCGCG ACAAAATGAA CTCCCCGGGT CTGCAGTCCA AGGAGCCCAG CAGCAGCCTG 
CACCTGGAAG GGTGGATGAA GGTGCCCAGG AATAACAAGC GAGGACAGCA AGGCTGGGAC 
AGGAAGTACA TCGTTCTGGA GGGCTCCAAA GTCCTCATTT ATGACAACGA AGCCAGAGAA 
GCTGGACAGA GGCCGGTGGA AGAATTTGAG CTGTGCCTTC CCGACGGGGA CGTGTCTATT 
CATGGCGCCG TTGGTGCTTC TGAACTTGCA AACACAGCCA AAGCAGATGT CCCCTATGTC 
CTGAAGATGG AGTCTCACCC CCATACCACC TGCTGGCCTG GGAGGACCCT CTACTTGCTG 
GCTCCCAGCT TTCCCGACAA GCAGCGCTGG GTCACCGCCT TAGAGTCAGT TGTGGCAGGT 
GGGAGAGTGT CTAGGGAAAA GGCGGAAGCC GACGCTGGCC GTGACTGTAC TTCCTACGAG 
CCTCTGCCCG CGTGGGCTCA GAAATTGCTC GGAAACTCCC TGTTGAAACT GGAAGGTGAT 
GACCGTCTGG ACATGAACTG CACGCTGCCC TTCAGCGACC AGGTGGTGCT GGTGGGCACC 
GAGGAAGGGC TGTATGCGCT GAATGTCTTG AAGAACTCCC TCACCCACGT CCCAGGAATC 
GGAGCAGTCT TCCAGATTTA CATCATCAAG GACCTGGAGA AGCTCCTCAT GATCGCAGGA 
GAGGAGCGGG CCCTGTGTCT CGTGGATGTG AAGAAGGTGA AGCAGTCCCT CGCGCAGTCT 
CACCTCCCCG CCCAGCCAGA CATCTCGCCC AACATCTTTG AAGCGGTCAA GGGCTGCCAC 
TTGTTTGCTG CTGGCAAGAT TGAGAGCGGG CTCTGCATCT GTGCAGCCAT GCCCAGCAAA 
GTTGTCATTC TCCGCTACAA TGAAAACCTC AGCAAGTACT GCATTCGCAA AGAGATCGAG 
ACCTCGGAGC CCTGCAGCTG TATCCACTTC ACCAACTACA GTATCCTCAT CGGAACCAAT 
AAATTCTACG AAATCGACAT GAAGCAGTAC ACGCTAGAGG AATTCCTGGA TAAGAACGAC 
CATTCCTTGG CGCCTGCCGT GTTTGCCTCC TCTTCCAACA GCTTCCCCGT GTCCATCATG 
CAGGTGAACG GCGCGGGGCA GCGGGAGGAG TTCCTGCTCT GTTTCCACGA ATTCGGGGTG 
TTCGTGGATT CTTATGGAAG ACGCAGCCGC ACAGATGATC TCAAGTGGAG TCGCTTACCT 
CTGGCCTTTG CCTACAGAGA ACCCTATCTG TTTGTGACTC ACTTCAACTC ACTCGAAGTA 
ATTGAGATCC AGGCCCGCTC CTCTCTGGGG ACCCCCGCCC GAGCATATTT GGAAATCCCC 
AACCCACGCT ACCTGGGCCC TGCAATCTCC TCAGGAGCCA TCTACCTGGC GTCCTCATAT 
CAGGATAAGT TAAGGGTCAT CTGCTGCAAA GGAAACCTGG TGAAGGAGAC CGGCACCGAC 
CACCACCGTG GCCCATCCAC CTCCCGCAGC CCCAACAAGC GAGGCCCGCC GACGTACAAC 
GAGCACATCA CCAAGCGCGT GGCCTCAAGC CCGGCGCCTC CCGAAGGCCC CAGCCACCCT 
CGAGAGCCAA GCACACCCCA CCGCTACCGC GAGGGGCGGA CCGAGCTGCG CAGGGACAAG 
TCTCCGGGCC GCCCCCTGGA GCGCGAGAAG TCCCCCGGCC GGATGCTGAG CACGCGGAGG 
GAGCGCTCCC CCGGGCGGCT GTTTGAGGAC AGCAGCAGGG GCCGGCTGCC CGTGGGGGCC 
GTGAGGACCC CGCTGTCCCA GGTCAACAAG GTCTGGGACC AGTCTTCAGT ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024833506.1
CDS	113..6166
Translation	MLKFKYGARNLLDAGTVESIASRASRLNLFFQGKPPSMTQQQMSLLSREAILDALFVLFKECSQPALMKIKHVSNFVQKYSDIIAELQELQPSVKDFEVKSVVGCGHFAEVQVVREKATGDVYAMKVMKKKALLAQEQVSYFEEEQNILSQSTSPWIPQLQYAFQDKNNLYLVMEYQPGGDLLSLLNRYEDQLDENMIQFYLAELILAVHSVHQMGYVHRDIKPENILIDRTGHIKLVDFGSAAKMNSNKMVNAKLPVGTPDYMAPEVLTVMNGDGKGAYSLDCDWWSVGVIAYEMVYGRCPFTEGTSAKTFNNIMNFQRFLKFPDDPKVSSELLDLIQSLLCGQKERLKFEGLCCHPFFSKIDWINIRNSPPPFVPTLKSDDDTSNFDEPEKNSWVSSSPCQLNPSGFSGEELPFVGFSYSKALGILGRSEPVVSSLDSPAKTSSMEKKLLIKSKELQDSQDKCHKMEQEMTRLHRRVSEVEAVLSQKEVELKASETQRSLLEQDLATYITECSSLKRSLEQARMEVSQEDDKALQLLHDIREQSRKLQEIKEQEYQAQVEEMRLMMNQLEEDLVSARRRSDLYESELRESRLAAEEFKRKATECQHKLMKAKDQGKPEVGEYAKLEKINAEQQLKIQELQEKLEKAVKASTEATELLQNIRQAKERAERELEKLHNREDSSEGIKKKLVEAEERRHSLENKVKRLETMERRENRLKDDIQTKSQQIQQMADKILELEEKHREAQVSAQHLEVHLKQKEQHYEEKIKVLDNQIKKDLADKETLENLMQRHEEEAHEKGKILSEQKAMINAMDSKIRSLEQRIVELSEANKLAANSSLFTQRNMKAQEEMISELRQQKFYLETQAGKLEAQNRKLEEQLEKISHQDHSDKNRLLELETRLREAHRDEIQRKFDALRNSCTVITDLEEQLNQLTEDNAELNNQNFYLSKQLDEASGANDEIVQLRSEVDHLRREITEREMQLTSQKQTMEALKTTCTMLEEQVMDLEALNDELLEKERQWEAWRSVLGDEKSQFECRVRELQRMLDTEKQSRARADQRITESRQVVELAVKEHKAEILALQQALKEQKLKAESLSDKLNDLEKKHAMLEMNARSLQQKLETERELKQRLLEEQAKLQQQMDLQKNHIFRLTQGLQEALDRADLLKTERSDLEYQLENIQVLYSHEKVKMEGTISQQTKLIDFLQAKMDQPAKKKKGLFSRRKEDPALPTQVPLQYNELKVALEKEKARCAELEEALQKTRIELRSAREEAAHRKAADHPHPSTPATARQQIAMSAIVRSPEHQPSALSLLAPPSSRRKESSTPEEFSRRLKERMHHNIPHRFNVGLNMRATKCAVCLDTVHFGRQASKCLECQVMCHPKCSTCLPATCGLPAEYATHFTEAFCRDKMNSPGLQSKEPSSSLHLEGWMKVPRNNKRGQQGWDRKYIVLEGSKVLIYDNEAREAGQRPVEEFELCLPDGDVSIHGAVGASELANTAKADVPYVLKMESHPHTTCWPGRTLYLLAPSFPDKQRWVTALESVVAGGRVSREKAEADAGRDCTSYEPLPAWAQKLLGNSLLKLEGDDRLDMNCTLPFSDQVVLVGTEEGLYALNVLKNSLTHVPGIGAVFQIYIIKDLEKLLMIAGEERALCLVDVKKVKQSLAQSHLPAQPDISPNIFEAVKGCHLFAAGKIESGLCICAAMPSKVVILRYNENLSKYCIRKEIETSEPCSCIHFTNYSILIGTNKFYEIDMKQYTLEEFLDKNDHSLAPAVFASSSNSFPVSIMQVNGAGQREEFLLCFHEFGVFVDSYGRRSRTDDLKWSRLPLAFAYREPYLFVTHFNSLEVIEIQARSSLGTPARAYLEIPNPRYLGPAISSGAIYLASSYQDKLRVICCKGNLVKETGTDHHRGPSTSRSPNKRGPPTYNEHITKRVASSPAPPEGPSHPREPSTPHRYREGRTELRRDKSPGRPLEREKSPGRMLSTRRERSPGRLFEDSSRGRLPVGAVRTPLSQVNKVWDQSSV

Target ORF information:

RefSeq Version	XM_024977738.1
Organism	Bos taurus(cattle)
Definition	Bos taurus citron rho-interacting serine/threonine kinase (CIT), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024977738.1

ORF Insert Sequence:

ATGTTGAAGT TCAAGTATGG AGCACGGAAT CTACTGGACG CTGGTACTGT TGAATCCATT 
GCCAGCCGGG CCTCCAGGCT GAATCTTTTC TTCCAGGGGA AACCACCCTC CATGACTCAA 
CAGCAGATGT CTCTTCTTTC CCGAGAAGCG ATATTAGATG CCCTCTTTGT TCTCTTTAAA 
GAATGCAGTC AGCCAGCTCT GATGAAGATT AAGCACGTGA GCAACTTTGT CCAAAAGTAT 
TCCGATATCA TAGCTGAGTT GCAAGAGCTC CAGCCTTCAG TGAAGGACTT TGAAGTGAAA 
AGTGTCGTAG GCTGCGGTCA CTTTGCTGAA GTACAGGTGG TCAGAGAGAA AGCGACTGGG 
GATGTCTATG CCATGAAAGT CATGAAGAAG AAGGCCTTGT TGGCCCAGGA GCAGGTCTCA 
TATTTTGAGG AAGAACAGAA CATATTATCT CAGAGTACAA GTCCTTGGAT CCCCCAGTTG 
CAGTATGCCT TTCAGGACAA AAATAACCTT TATCTGGTCA TGGAATATCA GCCTGGAGGG 
GATTTGCTGT CACTTTTGAA TAGATATGAG GACCAATTAG ATGAAAATAT GATTCAGTTT 
TACCTAGCTG AACTGATTTT GGCTGTCCAC AGCGTTCATC AGATGGGCTA TGTACATCGA 
GACATCAAGC CCGAGAACAT CCTCATTGAC CGAACGGGAC ACATCAAGCT GGTGGATTTT 
GGATCAGCTG CTAAAATGAA CTCAAATAAG ATGGTAAATG CCAAACTCCC GGTTGGTACT 
CCAGATTACA TGGCCCCTGA AGTGTTGACT GTGATGAATG GGGATGGAAA AGGTGCCTAT 
AGCCTAGACT GTGATTGGTG GTCAGTGGGA GTTATTGCTT ATGAGATGGT TTATGGAAGA 
TGCCCATTCA CTGAAGGAAC CTCAGCCAAA ACCTTCAATA ACATCATGAA TTTCCAGCGG 
TTCTTGAAGT TTCCAGATGA CCCCAAAGTT AGCAGTGAAT TGCTTGATCT AATCCAGAGT 
TTGTTGTGTG GCCAGAAAGA GAGACTGAAG TTTGAGGGCC TTTGCTGTCA TCCTTTCTTC 
TCCAAAATTG ACTGGATTAA CATTCGTAAC TCTCCTCCCC CCTTCGTTCC CACCCTCAAG 
TCTGATGATG ACACCTCCAA TTTTGATGAA CCAGAGAAGA ATTCGTGGGT TTCATCCTCT 
CCGTGCCAGC TGAACCCCTC AGGTTTCTCG GGCGAAGAAC TGCCGTTTGT GGGGTTTTCG 
TATAGCAAGG CACTGGGAAT TCTTGGTAGA TCTGAGCCTG TTGTATCAAG TCTGGACTCC 
CCTGCCAAGA CTAGCTCCAT GGAAAAGAAA CTCCTTATCA AAAGCAAAGA GCTGCAGGAC 
TCCCAGGACA AGTGTCACAA GATGGAGCAG GAAATGACCC GGTTACATCG GAGAGTGTCA 
GAGGTGGAGG CTGTGCTTAG TCAGAAGGAG GTGGAGCTGA AGGCCTCTGA GACTCAGAGA 
TCCCTCCTGG AGCAGGACCT TGCTACCTAC ATCACAGAAT GCAGTAGCTT AAAGCGAAGT 
TTGGAGCAAG CACGGATGGA GGTGTCCCAA GAGGACGACA AAGCACTGCA GCTCCTCCAT 
GATATCAGAG AGCAGAGCCG GAAGCTCCAA GAAATCAAAG AGCAGGAGTA CCAGGCTCAA 
GTGGAAGAAA TGAGGTTGAT GATGAATCAG TTGGAAGAGG ACCTGGTTTC GGCGAGAAGG 
CGGAGTGACC TCTATGAATC GGAGCTCAGA GAGTCTCGGC TGGCTGCTGA AGAGTTTAAG 
CGGAAAGCAA CAGAATGTCA GCATAAACTG ATGAAGGCTA AAGATCAAGG GAAGCCTGAA 
GTGGGAGAAT ATGCCAAACT GGAGAAGATC AATGCTGAGC AGCAGCTCAA AATTCAGGAG 
CTCCAAGAGA AGCTAGAAAA GGCTGTGAAA GCTAGCACAG AGGCCACCGA GCTGCTGCAG 
AACATCCGTC AGGCGAAGGA GCGGGCCGAG CGTGAGCTGG AGAAGCTGCA CAACCGTGAG 
GACTCCTCCG AGGGCATCAA GAAGAAGCTG GTGGAGGCCG AGGAACGCCG CCACTCTCTG 
GAGAACAAGG TAAAGAGGCT AGAGACCATG GAGCGTAGAG AAAACAGACT GAAGGATGAC 
ATCCAGACAA AGTCCCAACA GATCCAGCAG ATGGCTGATA AAATTCTGGA GCTGGAGGAG 
AAGCACAGAG AAGCCCAGGT CTCAGCGCAG CATCTGGAGG TGCACCTGAA ACAGAAAGAG 
CAGCACTATG AAGAAAAAAT TAAAGTGTTG GACAATCAGA TAAAGAAAGA CCTAGCAGAC 
AAGGAGACAC TGGAGAACCT GATGCAAAGA CACGAAGAGG AGGCCCATGA GAAAGGCAAA 
ATTCTCAGCG AGCAGAAGGC GATGATCAAT GCTATGGATT CCAAGATCAG ATCCCTGGAA 
CAAAGGATAG TGGAATTGTC GGAAGCCAAT AAACTTGCGG CCAACAGCAG TCTCTTTACC 
CAGAGGAACA TGAAGGCCCA GGAAGAGATG ATTTCAGAAC TCAGGCAACA GAAGTTTTAC 
CTGGAGACGC AAGCTGGGAA ATTGGAGGCC CAGAACCGGA AGCTGGAGGA GCAGCTGGAA 
AAAATAAGCC ACCAAGACCA CAGTGACAAG AACCGTCTGC TGGAGCTGGA GACCAGGCTA 
AGGGAGGCAC ATAGAGATGA AATCCAGCGC AAATTCGATG CCCTTCGTAA CAGCTGTACT 
GTGATCACGG ACTTGGAGGA GCAGCTAAAC CAGCTGACGG AGGACAATGC CGAGCTCAAC 
AACCAAAACT TCTACTTGTC TAAACAACTC GATGAGGCCT CTGGTGCCAA CGACGAGATT 
GTGCAGCTGC GAAGTGAAGT GGACCATCTC CGCCGCGAGA TCACTGAGAG GGAGATGCAG 
CTCACTAGCC AGAAGCAAAC GATGGAGGCG CTGAAGACCA CGTGCACAAT GCTGGAGGAG 
CAGGTCATGG ACCTGGAGGC CCTGAACGAC GAGCTGCTGG AGAAGGAGCG GCAGTGGGAG 
GCCTGGAGGA GTGTCCTTGG TGACGAGAAG TCCCAGTTTG AGTGTCGGGT TCGAGAGTTG 
CAGAGGATGC TGGACACCGA AAAGCAGAGC AGGGCGAGGG CCGACCAGAG GATCACTGAG 
TCGCGCCAGG TGGTCGAGCT GGCAGTAAAG GAGCACAAGG CCGAGATCCT GGCCTTGCAG 
CAGGCTCTCA AGGAGCAGAA GCTGAAGGCC GAGAGCCTCT CCGACAAGCT CAATGATCTG 
GAGAAGAAGC ACGCCATGCT TGAAATGAAT GCCCGAAGTT TACAACAGAA GCTGGAGACA 
GAACGAGAGC TCAAACAAAG GCTTCTGGAA GAGCAAGCCA AGTTACAGCA GCAGATGGAC 
CTGCAGAAGA ATCATATTTT CCGTCTGACT CAAGGGCTGC AAGAAGCTCT GGATCGGGCT 
GATCTGCTGA AGACAGAAAG GAGTGACCTG GAATATCAGC TGGAAAACAT TCAGGTTCTC 
TATTCTCATG AGAAGGTGAA GATGGAAGGC ACTATTTCCC AGCAAACCAA ACTCATCGAT 
TTTCTGCAAG CCAAAATGGA CCAGCCTGCC AAAAAGAAAA AGGGTTTATT TAGTCGACGG 
AAAGAGGACC CTGCTTTGCC CACACAGGTT CCTCTGCAGT ACAATGAGCT GAAGGTGGCT 
CTGGAGAAGG AGAAAGCTCG CTGCGCGGAG CTCGAGGAAG CCCTGCAGAA GACCCGCATC 
GAGCTCCGCT CCGCCCGGGA GGAAGCTGCC CACCGGAAAG CTGCAGACCA CCCACACCCA 
TCTACGCCGG CTACCGCCAG GCAGCAGATC GCCATGTCCG CCATTGTGCG GTCACCCGAG 
CACCAGCCCA GCGCCCTGAG CCTGCTCGCC CCGCCGTCCA GCCGCAGGAA GGAGTCTTCC 
ACTCCAGAGG AATTCAGCCG GCGTCTTAAG GAGCGCATGC ACCACAACAT CCCTCACCGG 
TTCAACGTGG GACTGAACAT GCGAGCCACG AAGTGCGCCG TGTGTCTGGA TACCGTGCAC 
TTCGGGCGCC AGGCATCCAA GTGTCTCGAA TGTCAGGTCA TGTGCCACCC CAAGTGCTCC 
ACATGCTTGC CAGCCACCTG TGGCCTGCCA GCCGAATACG CCACACACTT CACCGAGGCC 
TTCTGCCGCG ACAAAATGAA CTCCCCGGGT CTGCAGTCCA AGGAGCCCAG CAGCAGCCTG 
CACCTGGAAG GGTGGATGAA GGTGCCCAGG AATAACAAGC GAGGACAGCA AGGCTGGGAC 
AGGAAGTACA TCGTTCTGGA GGGCTCCAAA GTCCTCATTT ATGACAACGA AGCCAGAGAA 
GCTGGACAGA GGCCGGTGGA AGAATTTGAG CTGTGCCTTC CCGACGGGGA CGTGTCTATT 
CATGGCGCCG TTGGTGCTTC TGAACTTGCA AACACAGCCA AAGCAGATGT CCCCTATGTC 
CTGAAGATGG AGTCTCACCC CCATACCACC TGCTGGCCTG GGAGGACCCT CTACTTGCTG 
GCTCCCAGCT TTCCCGACAA GCAGCGCTGG GTCACCGCCT TAGAGTCAGT TGTGGCAGGT 
GGGAGAGTGT CTAGGGAAAA GGCGGAAGCC GACGCTGGCC GTGACTGTAC TTCCTACGAG 
CCTCTGCCCG CGTGGGCTCA GAAATTGCTC GGAAACTCCC TGTTGAAACT GGAAGGTGAT 
GACCGTCTGG ACATGAACTG CACGCTGCCC TTCAGCGACC AGGTGGTGCT GGTGGGCACC 
GAGGAAGGGC TGTATGCGCT GAATGTCTTG AAGAACTCCC TCACCCACGT CCCAGGAATC 
GGAGCAGTCT TCCAGATTTA CATCATCAAG GACCTGGAGA AGCTCCTCAT GATCGCAGGA 
GAGGAGCGGG CCCTGTGTCT CGTGGATGTG AAGAAGGTGA AGCAGTCCCT CGCGCAGTCT 
CACCTCCCCG CCCAGCCAGA CATCTCGCCC AACATCTTTG AAGCGGTCAA GGGCTGCCAC 
TTGTTTGCTG CTGGCAAGAT TGAGAGCGGG CTCTGCATCT GTGCAGCCAT GCCCAGCAAA 
GTTGTCATTC TCCGCTACAA TGAAAACCTC AGCAAGTACT GCATTCGCAA AGAGATCGAG 
ACCTCGGAGC CCTGCAGCTG TATCCACTTC ACCAACTACA GTATCCTCAT CGGAACCAAT 
AAATTCTACG AAATCGACAT GAAGCAGTAC ACGCTAGAGG AATTCCTGGA TAAGAACGAC 
CATTCCTTGG CGCCTGCCGT GTTTGCCTCC TCTTCCAACA GCTTCCCCGT GTCCATCATG 
CAGGTGAACG GCGCGGGGCA GCGGGAGGAG TTCCTGCTCT GTTTCCACGA ATTCGGGGTG 
TTCGTGGATT CTTATGGAAG ACGCAGCCGC ACAGATGATC TCAAGTGGAG TCGCTTACCT 
CTGGCCTTTG CCTACAGAGA ACCCTATCTG TTTGTGACTC ACTTCAACTC ACTCGAAGTA 
ATTGAGATCC AGGCCCGCTC CTCTCTGGGG ACCCCCGCCC GAGCATATTT GGAAATCCCC 
AACCCACGCT ACCTGGGCCC TGCAATCTCC TCAGGAGCCA TCTACCTGGC GTCCTCATAT 
CAGGATAAGT TAAGGGTCAT CTGCTGCAAA GGAAACCTGG TGAAGGAGAC CGGCACCGAC 
CACCACCGTG GCCCATCCAC CTCCCGCAGC CCCAACAAGC GAGGCCCGCC GACGTACAAC 
GAGCACATCA CCAAGCGCGT GGCCTCAAGC CCGGCGCCTC CCGAAGGCCC CAGCCACCCT 
CGAGAGCCAA GCACACCCCA CCGCTACCGC GAGGGGCGGA CCGAGCTGCG CAGGGACAAG 
TCTCCGGGCC GCCCCCTGGA GCGCGAGAAG TCCCCCGGCC GGATGCTGAG CACGCGGAGG 
GAGCGCTCCC CCGGGCGGCT GTTTGAGGAC AGCAGCAGGG GCCGGCTGCC CGTGGGGGCC 
GTGAGGACCC CGCTGTCCCA GGTCAACAAG GTCTGGGACC AGTCTTCAGT ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	CIT
Entrez Gene ID	786852
Full Name	citron rho-interacting serine/threonine kinase
General protein information	Preferred Names citron rho-interacting serine/threonine kinase Names citron Rho-interacting kinase citron (rho-interacting, serine/threonine kinase 21)
Gene Type	protein-coding
Organism	Bos taurus(cattle)

Homo sapiens (human)	CIT	NP_001193928.1
Pan troglodytes (chimpanzee)	CIT	XP_003952361.1
Bos taurus (cattle)	CIT	XP_002694628.3
Rattus norvegicus (Norway rat)	Cit	NP_001025082.1
Drosophila melanogaster (fruit fly)	sti	NP_001261768.1
Canis lupus familiaris (dog)	CIT	XP_003433432.1
Mus musculus (house mouse)	Cit	NP_031734.3
Danio rerio (zebrafish)	LOC100329792	XP_005171785.1
	CIT	XP_001085560.1
Gallus gallus (chicken)	CIT	XP_004934462.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006932	XP_308829.4
Xenopus tropicalis (tropical clawed frog)	cit	NP_001120601.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

CIT cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

見積依頼

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