CIT cDNA ORF clone, Bos taurus(cattle)

The following CIT gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CIT cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa224627 XM_024977739.1
Latest version!
Bos taurus citron rho-interacting serine/threonine kinase (CIT), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa224628 XM_024977740.1
Latest version!
Bos taurus citron rho-interacting serine/threonine kinase (CIT), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa224629 XM_024977741.1
Latest version!
Bos taurus citron rho-interacting serine/threonine kinase (CIT), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa224630 XM_024977743.1
Latest version!
Bos taurus citron rho-interacting serine/threonine kinase (CIT), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBa224630 XM_024977742.1
Latest version!
Bos taurus citron rho-interacting serine/threonine kinase (CIT), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBa224631 XM_024977744.1
Latest version!
Bos taurus citron rho-interacting serine/threonine kinase (CIT), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBa93236 XM_015467110.1
Latest version!
Bos taurus citron rho-interacting serine/threonine kinase (CIT), mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OBa93236 XM_015475534.1
Latest version!
Bos taurus citron rho-interacting serine/threonine kinase (CIT), mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OBa224622 XM_024977734.1
Latest version!
Bos taurus citron rho-interacting serine/threonine kinase (CIT), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OBa224622 XM_024977733.1
Latest version!
Bos taurus citron rho-interacting serine/threonine kinase (CIT), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OBa224623 XM_024977735.1
Latest version!
Bos taurus citron rho-interacting serine/threonine kinase (CIT), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OBa224624 XM_024977736.1
Latest version!
Bos taurus citron rho-interacting serine/threonine kinase (CIT), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OBa224625 XM_024977737.1
Latest version!
Bos taurus citron rho-interacting serine/threonine kinase (CIT), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OBa224626 XM_024977738.1
Latest version!
Bos taurus citron rho-interacting serine/threonine kinase (CIT), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa224627
    Clone ID Related Accession (Same CDS sequence) XM_024977739.1
    Accession Version XM_024977739.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5880bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product citron Rho-interacting kinase isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    ATGAAAGTCA TGAAGAAGAA GGCCTTGTTG GCCCAGGAGC AGGTCTCATA TTTTGAGGAA 
    GAACAGAACA TATTATCTCA GAGTACAAGT CCTTGGATCC CCCAGTTGCA GTATGCCTTT
    CAGGACAAAA ATAACCTTTA TCTGGTCATG GAATATCAGC CTGGAGGGGA TTTGCTGTCA
    CTTTTGAATA GATATGAGGA CCAATTAGAT GAAAATATGA TTCAGTTTTA CCTAGCTGAA
    CTGATTTTGG CTGTCCACAG CGTTCATCAG ATGGGCTATG TACATCGAGA CATCAAGCCC
    GAGAACATCC TCATTGACCG AACGGGACAC ATCAAGCTGG TGGATTTTGG ATCAGCTGCT
    AAAATGAACT CAAATAAGAT GGTAAATGCC AAACTCCCGG TTGGTACTCC AGATTACATG
    GCCCCTGAAG TGTTGACTGT GATGAATGGG GATGGAAAAG GTGCCTATAG CCTAGACTGT
    GATTGGTGGT CAGTGGGAGT TATTGCTTAT GAGATGGTTT ATGGAAGATG CCCATTCACT
    GAAGGAACCT CAGCCAAAAC CTTCAATAAC ATCATGAATT TCCAGCGGTT CTTGAAGTTT
    CCAGATGACC CCAAAGTTAG CAGTGAATTG CTTGATCTAA TCCAGAGTTT GTTGTGTGGC
    CAGAAAGAGA GACTGAAGTT TGAGGGCCTT TGCTGTCATC CTTTCTTCTC CAAAATTGAC
    TGGATTAACA TTCGTAACTC TCCTCCCCCC TTCGTTCCCA CCCTCAAGTC TGATGATGAC
    ACCTCCAATT TTGATGAACC AGAGAAGAAT TCGTGGGTTT CATCCTCTCC GTGCCAGCTG
    AACCCCTCAG GTTTCTCGGG CGAAGAACTG CCGTTTGTGG GGTTTTCGTA TAGCAAGGCA
    CTGGGAATTC TTGGTAGATC TGAGCCTGTT GTATCAAGTC TGGACTCCCC TGCCAAGACT
    AGCTCCATGG AAAAGAAACT CCTTATCAAA AGCAAAGAGC TGCAGGACTC CCAGGACAAG
    TGTCACAAGA TGGAGCAGGA AATGACCCGG TTACATCGGA GAGTGTCAGA GGTGGAGGCT
    GTGCTTAGTC AGAAGGAGGT GGAGCTGAAG GCCTCTGAGA CTCAGAGATC CCTCCTGGAG
    CAGGACCTTG CTACCTACAT CACAGAATGC AGTAGCTTAA AGCGAAGTTT GGAGCAAGCA
    CGGATGGAGG TGTCCCAAGA GGACGACAAA GCACTGCAGC TCCTCCATGA TATCAGAGAG
    CAGAGCCGGA AGCTCCAAGA AATCAAAGAG CAGGAGTACC AGGCTCAAGT GGAAGAAATG
    AGGTTGATGA TGAATCAGTT GGAAGAGGAC CTGGTTTCGG CGAGAAGGCG GAGTGACCTC
    TATGAATCGG AGCTCAGAGA GTCTCGGCTG GCTGCTGAAG AGTTTAAGCG GAAAGCAACA
    GAATGTCAGC ATAAACTGAT GAAGGCTAAA GATCAAGGGA AGCCTGAAGT GGGAGAATAT
    GCCAAACTGG AGAAGATCAA TGCTGAGCAG CAGCTCAAAA TTCAGGAGCT CCAAGAGAAG
    CTAGAAAAGG CTGTGAAAGC TAGCACAGAG GCCACCGAGC TGCTGCAGAA CATCCGTCAG
    GCGAAGGAGC GGGCCGAGCG TGAGCTGGAG AAGCTGCACA ACCGTGAGGA CTCCTCCGAG
    GGCATCAAGA AGAAGCTGGT GGAGGCCGAG GAACGCCGCC ACTCTCTGGA GAACAAGGTA
    AAGAGGCTAG AGACCATGGA GCGTAGAGAA AACAGACTGA AGGATGACAT CCAGACAAAG
    TCCCAACAGA TCCAGCAGAT GGCTGATAAA ATTCTGGAGC TGGAGGAGAA GCACAGAGAA
    GCCCAGGTCT CAGCGCAGCA TCTGGAGGTG CACCTGAAAC AGAAAGAGCA GCACTATGAA
    GAAAAAATTA AAGTGTTGGA CAATCAGATA AAGAAAGACC TAGCAGACAA GGAGACACTG
    GAGAACCTGA TGCAAAGACA CGAAGAGGAG GCCCATGAGA AAGGCAAAAT TCTCAGCGAG
    CAGAAGGCGA TGATCAATGC TATGGATTCC AAGATCAGAT CCCTGGAACA AAGGATAGTG
    GAATTGTCGG AAGCCAATAA ACTTGCGGCC AACAGCAGTC TCTTTACCCA GAGGAACATG
    AAGGCCCAGG AAGAGATGAT TTCAGAACTC AGGCAACAGA AGTTTTACCT GGAGACGCAA
    GCTGGGAAAT TGGAGGCCCA GAACCGGAAG CTGGAGGAGC AGCTGGAAAA AATAAGCCAC
    CAAGACCACA GTGACAAGAA CCGTCTGCTG GAGCTGGAGA CCAGGCTAAG GGAGGTCAGT
    CTAGAACATG AAGAGCAGAA ACTGGAGCTC AAACGCCAGC TCACAGAGCT GCAGCTCTCC
    CTGCAGGAGC GTGAATCCCA GCTGACAGCC CTGCAGGCAG CACGGGCGGC CCTGGAGAGC
    CAGCTCCGCC AGGCCAAGAC GGAGCTGGAA GAGACGACAG CAGAAGCAGA AGAAGAGATC
    CAGGCGCTTA CGGCACATAG AGATGAAATC CAGCGCAAAT TCGATGCCCT TCGTAACAGC
    TGTACTGTGA TCACGGACTT GGAGGAGCAG CTAAACCAGC TGACGGAGGA CAATGCCGAG
    CTCAACAACC AAAACTTCTA CTTGTCTAAA CAACTCGATG AGGCCTCTGG TGCCAACGAC
    GAGATTGTGC AGCTGCGAAG TGAAGTGGAC CATCTCCGCC GCGAGATCAC TGAGAGGGAG
    ATGCAGCTCA CTAGCCAGAA GCAAACGATG GAGGCGCTGA AGACCACGTG CACAATGCTG
    GAGGAGCAGG TCATGGACCT GGAGGCCCTG AACGACGAGC TGCTGGAGAA GGAGCGGCAG
    TGGGAGGCCT GGAGGAGTGT CCTTGGTGAC GAGAAGTCCC AGTTTGAGTG TCGGGTTCGA
    GAGTTGCAGA GGATGCTGGA CACCGAAAAG CAGAGCAGGG CGAGGGCCGA CCAGAGGATC
    ACTGAGTCGC GCCAGGTGGT CGAGCTGGCA GTAAAGGAGC ACAAGGCCGA GATCCTGGCC
    TTGCAGCAGG CTCTCAAGGA GCAGAAGCTG AAGGCCGAGA GCCTCTCCGA CAAGCTCAAT
    GATCTGGAGA AGAAGCACGC CATGCTTGAA ATGAATGCCC GAAGTTTACA ACAGAAGCTG
    GAGACAGAAC GAGAGCTCAA ACAAAGGCTT CTGGAAGAGC AAGCCAAGTT ACAGCAGCAG
    ATGGACCTGC AGAAGAATCA TATTTTCCGT CTGACTCAAG GGCTGCAAGA AGCTCTGGAT
    CGGGCTGATC TGCTGAAGAC AGAAAGGAGT GACCTGGAAT ATCAGCTGGA AAACATTCAG
    GTTCTCTATT CTCATGAGAA GGTGAAGATG GAAGGCACTA TTTCCCAGCA AACCAAACTC
    ATCGATTTTC TGCAAGCCAA AATGGACCAG CCTGCCAAAA AGAAAAAGGG TTTATTTAGT
    CGACGGAAAG AGGACCCTGC TTTGCCCACA CAGGTTCCTC TGCAGTACAA TGAGCTGAAG
    GTGGCTCTGG AGAAGGAGAA AGCTCGCTGC GCGGAGCTCG AGGAAGCCCT GCAGAAGACC
    CGCATCGAGC TCCGCTCCGC CCGGGAGGAA GCTGCCCACC GGAAAGCTGC AGACCACCCA
    CACCCATCTA CGCCGGCTAC CGCCAGGCAG CAGATCGCCA TGTCCGCCAT TGTGCGGTCA
    CCCGAGCACC AGCCCAGCGC CCTGAGCCTG CTCGCCCCGC CGTCCAGCCG CAGGAAGGAG
    TCTTCCACTC CAGAGGAATT CAGCCGGCGT CTTAAGGAGC GCATGCACCA CAACATCCCT
    CACCGGTTCA ACGTGGGACT GAACATGCGA GCCACGAAGT GCGCCGTGTG TCTGGATACC
    GTGCACTTCG GGCGCCAGGC ATCCAAGTGT CTCGAATGTC AGGTCATGTG CCACCCCAAG
    TGCTCCACAT GCTTGCCAGC CACCTGTGGC CTGCCAGCCG AATACGCCAC ACACTTCACC
    GAGGCCTTCT GCCGCGACAA AATGAACTCC CCGGGTCTGC AGTCCAAGGA GCCCAGCAGC
    AGCCTGCACC TGGAAGGGTG GATGAAGGTG CCCAGGAATA ACAAGCGAGG ACAGCAAGGC
    TGGGACAGGA AGTACATCGT TCTGGAGGGC TCCAAAGTCC TCATTTATGA CAACGAAGCC
    AGAGAAGCTG GACAGAGGCC GGTGGAAGAA TTTGAGCTGT GCCTTCCCGA CGGGGACGTG
    TCTATTCATG GCGCCGTTGG TGCTTCTGAA CTTGCAAACA CAGCCAAAGC AGATGTCCCC
    TATGTCCTGA AGATGGAGTC TCACCCCCAT ACCACCTGCT GGCCTGGGAG GACCCTCTAC
    TTGCTGGCTC CCAGCTTTCC CGACAAGCAG CGCTGGGTCA CCGCCTTAGA GTCAGTTGTG
    GCAGGTGGGA GAGTGTCTAG GGAAAAGGCG GAAGCCGACG CTGGCCGTGA CTGTACTTCC
    TACGAGCCTC TGCCCGCGTG GGCTCAGAAA TTGCTCGGAA ACTCCCTGTT GAAACTGGAA
    GGTGATGACC GTCTGGACAT GAACTGCACG CTGCCCTTCA GCGACCAGGT GGTGCTGGTG
    GGCACCGAGG AAGGGCTGTA TGCGCTGAAT GTCTTGAAGA ACTCCCTCAC CCACGTCCCA
    GGAATCGGAG CAGTCTTCCA GATTTACATC ATCAAGGACC TGGAGAAGCT CCTCATGATC
    GCAGGAGAGG AGCGGGCCCT GTGTCTCGTG GATGTGAAGA AGGTGAAGCA GTCCCTCGCG
    CAGTCTCACC TCCCCGCCCA GCCAGACATC TCGCCCAACA TCTTTGAAGC GGTCAAGGGC
    TGCCACTTGT TTGCTGCTGG CAAGATTGAG AGCGGGCTCT GCATCTGTGC AGCCATGCCC
    AGCAAAGTTG TCATTCTCCG CTACAATGAA AACCTCAGCA AGTACTGCAT TCGCAAAGAG
    ATCGAGACCT CGGAGCCCTG CAGCTGTATC CACTTCACCA ACTACAGTAT CCTCATCGGA
    ACCAATAAAT TCTACGAAAT CGACATGAAG CAGTACACGC TAGAGGAATT CCTGGATAAG
    AACGACCATT CCTTGGCGCC TGCCGTGTTT GCCTCCTCTT CCAACAGCTT CCCCGTGTCC
    ATCATGCAGG TGAACGGCGC GGGGCAGCGG GAGGAGTTCC TGCTCTGTTT CCACGAATTC
    GGGGTGTTCG TGGATTCTTA TGGAAGACGC AGCCGCACAG ATGATCTCAA GTGGAGTCGC
    TTACCTCTGG CCTTTGCCTA CAGAGAACCC TATCTGTTTG TGACTCACTT CAACTCACTC
    GAAGTAATTG AGATCCAGGC CCGCTCCTCT CTGGGGACCC CCGCCCGAGC ATATTTGGAA
    ATCCCCAACC CACGCTACCT GGGCCCTGCA ATCTCCTCAG GAGCCATCTA CCTGGCGTCC
    TCATATCAGG ATAAGTTAAG GGTCATCTGC TGCAAAGGAA ACCTGGTGAA GGAGACCGGC
    ACCGACCACC ACCGTGGCCC ATCCACCTCC CGCAGCCCCA ACAAGCGAGG CCCGCCGACG
    TACAACGAGC ACATCACCAA GCGCGTGGCC TCAAGCCCGG CGCCTCCCGA AGGCCCCAGC
    CACCCTCGAG AGCCAAGCAC ACCCCACCGC TACCGCGAGG GGCGGACCGA GCTGCGCAGG
    GACAAGTCTC CGGGCCGCCC CCTGGAGCGC GAGAAGTCCC CCGGCCGGAT GCTGAGCACG
    CGGAGGGAGC GCTCCCCCGG GCGGCTGTTT GAGGACAGCA GCAGGGGCCG GCTGCCCGTG
    GGGGCCGTGA GGACCCCGCT GTCCCAGGTC AACAAGGTCT GGGACCAGTC TTCAGTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024833507.1
    CDS238..6117
    Translation

    Target ORF information:

    RefSeq Version XM_024977739.1
    Organism Bos taurus(cattle)
    Definition Bos taurus citron rho-interacting serine/threonine kinase (CIT), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024977739.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    ATGAAAGTCA TGAAGAAGAA GGCCTTGTTG GCCCAGGAGC AGGTCTCATA TTTTGAGGAA 
    GAACAGAACA TATTATCTCA GAGTACAAGT CCTTGGATCC CCCAGTTGCA GTATGCCTTT
    CAGGACAAAA ATAACCTTTA TCTGGTCATG GAATATCAGC CTGGAGGGGA TTTGCTGTCA
    CTTTTGAATA GATATGAGGA CCAATTAGAT GAAAATATGA TTCAGTTTTA CCTAGCTGAA
    CTGATTTTGG CTGTCCACAG CGTTCATCAG ATGGGCTATG TACATCGAGA CATCAAGCCC
    GAGAACATCC TCATTGACCG AACGGGACAC ATCAAGCTGG TGGATTTTGG ATCAGCTGCT
    AAAATGAACT CAAATAAGAT GGTAAATGCC AAACTCCCGG TTGGTACTCC AGATTACATG
    GCCCCTGAAG TGTTGACTGT GATGAATGGG GATGGAAAAG GTGCCTATAG CCTAGACTGT
    GATTGGTGGT CAGTGGGAGT TATTGCTTAT GAGATGGTTT ATGGAAGATG CCCATTCACT
    GAAGGAACCT CAGCCAAAAC CTTCAATAAC ATCATGAATT TCCAGCGGTT CTTGAAGTTT
    CCAGATGACC CCAAAGTTAG CAGTGAATTG CTTGATCTAA TCCAGAGTTT GTTGTGTGGC
    CAGAAAGAGA GACTGAAGTT TGAGGGCCTT TGCTGTCATC CTTTCTTCTC CAAAATTGAC
    TGGATTAACA TTCGTAACTC TCCTCCCCCC TTCGTTCCCA CCCTCAAGTC TGATGATGAC
    ACCTCCAATT TTGATGAACC AGAGAAGAAT TCGTGGGTTT CATCCTCTCC GTGCCAGCTG
    AACCCCTCAG GTTTCTCGGG CGAAGAACTG CCGTTTGTGG GGTTTTCGTA TAGCAAGGCA
    CTGGGAATTC TTGGTAGATC TGAGCCTGTT GTATCAAGTC TGGACTCCCC TGCCAAGACT
    AGCTCCATGG AAAAGAAACT CCTTATCAAA AGCAAAGAGC TGCAGGACTC CCAGGACAAG
    TGTCACAAGA TGGAGCAGGA AATGACCCGG TTACATCGGA GAGTGTCAGA GGTGGAGGCT
    GTGCTTAGTC AGAAGGAGGT GGAGCTGAAG GCCTCTGAGA CTCAGAGATC CCTCCTGGAG
    CAGGACCTTG CTACCTACAT CACAGAATGC AGTAGCTTAA AGCGAAGTTT GGAGCAAGCA
    CGGATGGAGG TGTCCCAAGA GGACGACAAA GCACTGCAGC TCCTCCATGA TATCAGAGAG
    CAGAGCCGGA AGCTCCAAGA AATCAAAGAG CAGGAGTACC AGGCTCAAGT GGAAGAAATG
    AGGTTGATGA TGAATCAGTT GGAAGAGGAC CTGGTTTCGG CGAGAAGGCG GAGTGACCTC
    TATGAATCGG AGCTCAGAGA GTCTCGGCTG GCTGCTGAAG AGTTTAAGCG GAAAGCAACA
    GAATGTCAGC ATAAACTGAT GAAGGCTAAA GATCAAGGGA AGCCTGAAGT GGGAGAATAT
    GCCAAACTGG AGAAGATCAA TGCTGAGCAG CAGCTCAAAA TTCAGGAGCT CCAAGAGAAG
    CTAGAAAAGG CTGTGAAAGC TAGCACAGAG GCCACCGAGC TGCTGCAGAA CATCCGTCAG
    GCGAAGGAGC GGGCCGAGCG TGAGCTGGAG AAGCTGCACA ACCGTGAGGA CTCCTCCGAG
    GGCATCAAGA AGAAGCTGGT GGAGGCCGAG GAACGCCGCC ACTCTCTGGA GAACAAGGTA
    AAGAGGCTAG AGACCATGGA GCGTAGAGAA AACAGACTGA AGGATGACAT CCAGACAAAG
    TCCCAACAGA TCCAGCAGAT GGCTGATAAA ATTCTGGAGC TGGAGGAGAA GCACAGAGAA
    GCCCAGGTCT CAGCGCAGCA TCTGGAGGTG CACCTGAAAC AGAAAGAGCA GCACTATGAA
    GAAAAAATTA AAGTGTTGGA CAATCAGATA AAGAAAGACC TAGCAGACAA GGAGACACTG
    GAGAACCTGA TGCAAAGACA CGAAGAGGAG GCCCATGAGA AAGGCAAAAT TCTCAGCGAG
    CAGAAGGCGA TGATCAATGC TATGGATTCC AAGATCAGAT CCCTGGAACA AAGGATAGTG
    GAATTGTCGG AAGCCAATAA ACTTGCGGCC AACAGCAGTC TCTTTACCCA GAGGAACATG
    AAGGCCCAGG AAGAGATGAT TTCAGAACTC AGGCAACAGA AGTTTTACCT GGAGACGCAA
    GCTGGGAAAT TGGAGGCCCA GAACCGGAAG CTGGAGGAGC AGCTGGAAAA AATAAGCCAC
    CAAGACCACA GTGACAAGAA CCGTCTGCTG GAGCTGGAGA CCAGGCTAAG GGAGGTCAGT
    CTAGAACATG AAGAGCAGAA ACTGGAGCTC AAACGCCAGC TCACAGAGCT GCAGCTCTCC
    CTGCAGGAGC GTGAATCCCA GCTGACAGCC CTGCAGGCAG CACGGGCGGC CCTGGAGAGC
    CAGCTCCGCC AGGCCAAGAC GGAGCTGGAA GAGACGACAG CAGAAGCAGA AGAAGAGATC
    CAGGCGCTTA CGGCACATAG AGATGAAATC CAGCGCAAAT TCGATGCCCT TCGTAACAGC
    TGTACTGTGA TCACGGACTT GGAGGAGCAG CTAAACCAGC TGACGGAGGA CAATGCCGAG
    CTCAACAACC AAAACTTCTA CTTGTCTAAA CAACTCGATG AGGCCTCTGG TGCCAACGAC
    GAGATTGTGC AGCTGCGAAG TGAAGTGGAC CATCTCCGCC GCGAGATCAC TGAGAGGGAG
    ATGCAGCTCA CTAGCCAGAA GCAAACGATG GAGGCGCTGA AGACCACGTG CACAATGCTG
    GAGGAGCAGG TCATGGACCT GGAGGCCCTG AACGACGAGC TGCTGGAGAA GGAGCGGCAG
    TGGGAGGCCT GGAGGAGTGT CCTTGGTGAC GAGAAGTCCC AGTTTGAGTG TCGGGTTCGA
    GAGTTGCAGA GGATGCTGGA CACCGAAAAG CAGAGCAGGG CGAGGGCCGA CCAGAGGATC
    ACTGAGTCGC GCCAGGTGGT CGAGCTGGCA GTAAAGGAGC ACAAGGCCGA GATCCTGGCC
    TTGCAGCAGG CTCTCAAGGA GCAGAAGCTG AAGGCCGAGA GCCTCTCCGA CAAGCTCAAT
    GATCTGGAGA AGAAGCACGC CATGCTTGAA ATGAATGCCC GAAGTTTACA ACAGAAGCTG
    GAGACAGAAC GAGAGCTCAA ACAAAGGCTT CTGGAAGAGC AAGCCAAGTT ACAGCAGCAG
    ATGGACCTGC AGAAGAATCA TATTTTCCGT CTGACTCAAG GGCTGCAAGA AGCTCTGGAT
    CGGGCTGATC TGCTGAAGAC AGAAAGGAGT GACCTGGAAT ATCAGCTGGA AAACATTCAG
    GTTCTCTATT CTCATGAGAA GGTGAAGATG GAAGGCACTA TTTCCCAGCA AACCAAACTC
    ATCGATTTTC TGCAAGCCAA AATGGACCAG CCTGCCAAAA AGAAAAAGGG TTTATTTAGT
    CGACGGAAAG AGGACCCTGC TTTGCCCACA CAGGTTCCTC TGCAGTACAA TGAGCTGAAG
    GTGGCTCTGG AGAAGGAGAA AGCTCGCTGC GCGGAGCTCG AGGAAGCCCT GCAGAAGACC
    CGCATCGAGC TCCGCTCCGC CCGGGAGGAA GCTGCCCACC GGAAAGCTGC AGACCACCCA
    CACCCATCTA CGCCGGCTAC CGCCAGGCAG CAGATCGCCA TGTCCGCCAT TGTGCGGTCA
    CCCGAGCACC AGCCCAGCGC CCTGAGCCTG CTCGCCCCGC CGTCCAGCCG CAGGAAGGAG
    TCTTCCACTC CAGAGGAATT CAGCCGGCGT CTTAAGGAGC GCATGCACCA CAACATCCCT
    CACCGGTTCA ACGTGGGACT GAACATGCGA GCCACGAAGT GCGCCGTGTG TCTGGATACC
    GTGCACTTCG GGCGCCAGGC ATCCAAGTGT CTCGAATGTC AGGTCATGTG CCACCCCAAG
    TGCTCCACAT GCTTGCCAGC CACCTGTGGC CTGCCAGCCG AATACGCCAC ACACTTCACC
    GAGGCCTTCT GCCGCGACAA AATGAACTCC CCGGGTCTGC AGTCCAAGGA GCCCAGCAGC
    AGCCTGCACC TGGAAGGGTG GATGAAGGTG CCCAGGAATA ACAAGCGAGG ACAGCAAGGC
    TGGGACAGGA AGTACATCGT TCTGGAGGGC TCCAAAGTCC TCATTTATGA CAACGAAGCC
    AGAGAAGCTG GACAGAGGCC GGTGGAAGAA TTTGAGCTGT GCCTTCCCGA CGGGGACGTG
    TCTATTCATG GCGCCGTTGG TGCTTCTGAA CTTGCAAACA CAGCCAAAGC AGATGTCCCC
    TATGTCCTGA AGATGGAGTC TCACCCCCAT ACCACCTGCT GGCCTGGGAG GACCCTCTAC
    TTGCTGGCTC CCAGCTTTCC CGACAAGCAG CGCTGGGTCA CCGCCTTAGA GTCAGTTGTG
    GCAGGTGGGA GAGTGTCTAG GGAAAAGGCG GAAGCCGACG CTGGCCGTGA CTGTACTTCC
    TACGAGCCTC TGCCCGCGTG GGCTCAGAAA TTGCTCGGAA ACTCCCTGTT GAAACTGGAA
    GGTGATGACC GTCTGGACAT GAACTGCACG CTGCCCTTCA GCGACCAGGT GGTGCTGGTG
    GGCACCGAGG AAGGGCTGTA TGCGCTGAAT GTCTTGAAGA ACTCCCTCAC CCACGTCCCA
    GGAATCGGAG CAGTCTTCCA GATTTACATC ATCAAGGACC TGGAGAAGCT CCTCATGATC
    GCAGGAGAGG AGCGGGCCCT GTGTCTCGTG GATGTGAAGA AGGTGAAGCA GTCCCTCGCG
    CAGTCTCACC TCCCCGCCCA GCCAGACATC TCGCCCAACA TCTTTGAAGC GGTCAAGGGC
    TGCCACTTGT TTGCTGCTGG CAAGATTGAG AGCGGGCTCT GCATCTGTGC AGCCATGCCC
    AGCAAAGTTG TCATTCTCCG CTACAATGAA AACCTCAGCA AGTACTGCAT TCGCAAAGAG
    ATCGAGACCT CGGAGCCCTG CAGCTGTATC CACTTCACCA ACTACAGTAT CCTCATCGGA
    ACCAATAAAT TCTACGAAAT CGACATGAAG CAGTACACGC TAGAGGAATT CCTGGATAAG
    AACGACCATT CCTTGGCGCC TGCCGTGTTT GCCTCCTCTT CCAACAGCTT CCCCGTGTCC
    ATCATGCAGG TGAACGGCGC GGGGCAGCGG GAGGAGTTCC TGCTCTGTTT CCACGAATTC
    GGGGTGTTCG TGGATTCTTA TGGAAGACGC AGCCGCACAG ATGATCTCAA GTGGAGTCGC
    TTACCTCTGG CCTTTGCCTA CAGAGAACCC TATCTGTTTG TGACTCACTT CAACTCACTC
    GAAGTAATTG AGATCCAGGC CCGCTCCTCT CTGGGGACCC CCGCCCGAGC ATATTTGGAA
    ATCCCCAACC CACGCTACCT GGGCCCTGCA ATCTCCTCAG GAGCCATCTA CCTGGCGTCC
    TCATATCAGG ATAAGTTAAG GGTCATCTGC TGCAAAGGAA ACCTGGTGAA GGAGACCGGC
    ACCGACCACC ACCGTGGCCC ATCCACCTCC CGCAGCCCCA ACAAGCGAGG CCCGCCGACG
    TACAACGAGC ACATCACCAA GCGCGTGGCC TCAAGCCCGG CGCCTCCCGA AGGCCCCAGC
    CACCCTCGAG AGCCAAGCAC ACCCCACCGC TACCGCGAGG GGCGGACCGA GCTGCGCAGG
    GACAAGTCTC CGGGCCGCCC CCTGGAGCGC GAGAAGTCCC CCGGCCGGAT GCTGAGCACG
    CGGAGGGAGC GCTCCCCCGG GCGGCTGTTT GAGGACAGCA GCAGGGGCCG GCTGCCCGTG
    GGGGCCGTGA GGACCCCGCT GTCCCAGGTC AACAAGGTCT GGGACCAGTC TTCAGTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa224628
    Clone ID Related Accession (Same CDS sequence) XM_024977740.1
    Accession Version XM_024977740.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5517bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product citron Rho-interacting kinase isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGAACTCAA ATAAGATGGT AAATGCCAAA CTCCCGGTTG GTACTCCAGA TTACATGGCC 
    CCTGAAGTGT TGACTGTGAT GAATGGGGAT GGAAAAGGTG CCTATAGCCT AGACTGTGAT
    TGGTGGTCAG TGGGAGTTAT TGCTTATGAG ATGGTTTATG GAAGATGCCC ATTCACTGAA
    GGAACCTCAG CCAAAACCTT CAATAACATC ATGAATTTCC AGCGGTTCTT GAAGTTTCCA
    GATGACCCCA AAGTTAGCAG TGAATTGCTT GATCTAATCC AGAGTTTGTT GTGTGGCCAG
    AAAGAGAGAC TGAAGTTTGA GGGCCTTTGC TGTCATCCTT TCTTCTCCAA AATTGACTGG
    ATTAACATTC GTAACTCTCC TCCCCCCTTC GTTCCCACCC TCAAGTCTGA TGATGACACC
    TCCAATTTTG ATGAACCAGA GAAGAATTCG TGGGTTTCAT CCTCTCCGTG CCAGCTGAAC
    CCCTCAGGTT TCTCGGGCGA AGAACTGCCG TTTGTGGGGT TTTCGTATAG CAAGGCACTG
    GGAATTCTTG GTAGATCTGA GCCTGTTGTA TCAAGTCTGG ACTCCCCTGC CAAGACTAGC
    TCCATGGAAA AGAAACTCCT TATCAAAAGC AAAGAGCTGC AGGACTCCCA GGACAAGTGT
    CACAAGATGG AGCAGGAAAT GACCCGGTTA CATCGGAGAG TGTCAGAGGT GGAGGCTGTG
    CTTAGTCAGA AGGAGGTGGA GCTGAAGGCC TCTGAGACTC AGAGATCCCT CCTGGAGCAG
    GACCTTGCTA CCTACATCAC AGAATGCAGT AGCTTAAAGC GAAGTTTGGA GCAAGCACGG
    ATGGAGGTGT CCCAAGAGGA CGACAAAGCA CTGCAGCTCC TCCATGATAT CAGAGAGCAG
    AGCCGGAAGC TCCAAGAAAT CAAAGAGCAG GAGTACCAGG CTCAAGTGGA AGAAATGAGG
    TTGATGATGA ATCAGTTGGA AGAGGACCTG GTTTCGGCGA GAAGGCGGAG TGACCTCTAT
    GAATCGGAGC TCAGAGAGTC TCGGCTGGCT GCTGAAGAGT TTAAGCGGAA AGCAACAGAA
    TGTCAGCATA AACTGATGAA GGCTAAAGAT CAAGGGAAGC CTGAAGTGGG AGAATATGCC
    AAACTGGAGA AGATCAATGC TGAGCAGCAG CTCAAAATTC AGGAGCTCCA AGAGAAGCTA
    GAAAAGGCTG TGAAAGCTAG CACAGAGGCC ACCGAGCTGC TGCAGAACAT CCGTCAGGCG
    AAGGAGCGGG CCGAGCGTGA GCTGGAGAAG CTGCACAACC GTGAGGACTC CTCCGAGGGC
    ATCAAGAAGA AGCTGGTGGA GGCCGAGGAA CGCCGCCACT CTCTGGAGAA CAAGGTAAAG
    AGGCTAGAGA CCATGGAGCG TAGAGAAAAC AGACTGAAGG ATGACATCCA GACAAAGTCC
    CAACAGATCC AGCAGATGGC TGATAAAATT CTGGAGCTGG AGGAGAAGCA CAGAGAAGCC
    CAGGTCTCAG CGCAGCATCT GGAGGTGCAC CTGAAACAGA AAGAGCAGCA CTATGAAGAA
    AAAATTAAAG TGTTGGACAA TCAGATAAAG AAAGACCTAG CAGACAAGGA GACACTGGAG
    AACCTGATGC AAAGACACGA AGAGGAGGCC CATGAGAAAG GCAAAATTCT CAGCGAGCAG
    AAGGCGATGA TCAATGCTAT GGATTCCAAG ATCAGATCCC TGGAACAAAG GATAGTGGAA
    TTGTCGGAAG CCAATAAACT TGCGGCCAAC AGCAGTCTCT TTACCCAGAG GAACATGAAG
    GCCCAGGAAG AGATGATTTC AGAACTCAGG CAACAGAAGT TTTACCTGGA GACGCAAGCT
    GGGAAATTGG AGGCCCAGAA CCGGAAGCTG GAGGAGCAGC TGGAAAAAAT AAGCCACCAA
    GACCACAGTG ACAAGAACCG TCTGCTGGAG CTGGAGACCA GGCTAAGGGA GGTCAGTCTA
    GAACATGAAG AGCAGAAACT GGAGCTCAAA CGCCAGCTCA CAGAGCTGCA GCTCTCCCTG
    CAGGAGCGTG AATCCCAGCT GACAGCCCTG CAGGCAGCAC GGGCGGCCCT GGAGAGCCAG
    CTCCGCCAGG CCAAGACGGA GCTGGAAGAG ACGACAGCAG AAGCAGAAGA AGAGATCCAG
    GCGCTTACGG CACATAGAGA TGAAATCCAG CGCAAATTCG ATGCCCTTCG TAACAGCTGT
    ACTGTGATCA CGGACTTGGA GGAGCAGCTA AACCAGCTGA CGGAGGACAA TGCCGAGCTC
    AACAACCAAA ACTTCTACTT GTCTAAACAA CTCGATGAGG CCTCTGGTGC CAACGACGAG
    ATTGTGCAGC TGCGAAGTGA AGTGGACCAT CTCCGCCGCG AGATCACTGA GAGGGAGATG
    CAGCTCACTA GCCAGAAGCA AACGATGGAG GCGCTGAAGA CCACGTGCAC AATGCTGGAG
    GAGCAGGTCA TGGACCTGGA GGCCCTGAAC GACGAGCTGC TGGAGAAGGA GCGGCAGTGG
    GAGGCCTGGA GGAGTGTCCT TGGTGACGAG AAGTCCCAGT TTGAGTGTCG GGTTCGAGAG
    TTGCAGAGGA TGCTGGACAC CGAAAAGCAG AGCAGGGCGA GGGCCGACCA GAGGATCACT
    GAGTCGCGCC AGGTGGTCGA GCTGGCAGTA AAGGAGCACA AGGCCGAGAT CCTGGCCTTG
    CAGCAGGCTC TCAAGGAGCA GAAGCTGAAG GCCGAGAGCC TCTCCGACAA GCTCAATGAT
    CTGGAGAAGA AGCACGCCAT GCTTGAAATG AATGCCCGAA GTTTACAACA GAAGCTGGAG
    ACAGAACGAG AGCTCAAACA AAGGCTTCTG GAAGAGCAAG CCAAGTTACA GCAGCAGATG
    GACCTGCAGA AGAATCATAT TTTCCGTCTG ACTCAAGGGC TGCAAGAAGC TCTGGATCGG
    GCTGATCTGC TGAAGACAGA AAGGAGTGAC CTGGAATATC AGCTGGAAAA CATTCAGGTT
    CTCTATTCTC ATGAGAAGGT GAAGATGGAA GGCACTATTT CCCAGCAAAC CAAACTCATC
    GATTTTCTGC AAGCCAAAAT GGACCAGCCT GCCAAAAAGA AAAAGGGTTT ATTTAGTCGA
    CGGAAAGAGG ACCCTGCTTT GCCCACACAG GTTCCTCTGC AGTACAATGA GCTGAAGGTG
    GCTCTGGAGA AGGAGAAAGC TCGCTGCGCG GAGCTCGAGG AAGCCCTGCA GAAGACCCGC
    ATCGAGCTCC GCTCCGCCCG GGAGGAAGCT GCCCACCGGA AAGCTGCAGA CCACCCACAC
    CCATCTACGC CGGCTACCGC CAGGCAGCAG ATCGCCATGT CCGCCATTGT GCGGTCACCC
    GAGCACCAGC CCAGCGCCCT GAGCCTGCTC GCCCCGCCGT CCAGCCGCAG GAAGGAGTCT
    TCCACTCCAG AGGAATTCAG CCGGCGTCTT AAGGAGCGCA TGCACCACAA CATCCCTCAC
    CGGTTCAACG TGGGACTGAA CATGCGAGCC ACGAAGTGCG CCGTGTGTCT GGATACCGTG
    CACTTCGGGC GCCAGGCATC CAAGTGTCTC GAATGTCAGG TCATGTGCCA CCCCAAGTGC
    TCCACATGCT TGCCAGCCAC CTGTGGCCTG CCAGCCGAAT ACGCCACACA CTTCACCGAG
    GCCTTCTGCC GCGACAAAAT GAACTCCCCG GGTCTGCAGT CCAAGGAGCC CAGCAGCAGC
    CTGCACCTGG AAGGGTGGAT GAAGGTGCCC AGGAATAACA AGCGAGGACA GCAAGGCTGG
    GACAGGAAGT ACATCGTTCT GGAGGGCTCC AAAGTCCTCA TTTATGACAA CGAAGCCAGA
    GAAGCTGGAC AGAGGCCGGT GGAAGAATTT GAGCTGTGCC TTCCCGACGG GGACGTGTCT
    ATTCATGGCG CCGTTGGTGC TTCTGAACTT GCAAACACAG CCAAAGCAGA TGTCCCCTAT
    GTCCTGAAGA TGGAGTCTCA CCCCCATACC ACCTGCTGGC CTGGGAGGAC CCTCTACTTG
    CTGGCTCCCA GCTTTCCCGA CAAGCAGCGC TGGGTCACCG CCTTAGAGTC AGTTGTGGCA
    GGTGGGAGAG TGTCTAGGGA AAAGGCGGAA GCCGACGCTG GCCGTGACTG TACTTCCTAC
    GAGCCTCTGC CCGCGTGGGC TCAGAAATTG CTCGGAAACT CCCTGTTGAA ACTGGAAGGT
    GATGACCGTC TGGACATGAA CTGCACGCTG CCCTTCAGCG ACCAGGTGGT GCTGGTGGGC
    ACCGAGGAAG GGCTGTATGC GCTGAATGTC TTGAAGAACT CCCTCACCCA CGTCCCAGGA
    ATCGGAGCAG TCTTCCAGAT TTACATCATC AAGGACCTGG AGAAGCTCCT CATGATCGCA
    GGAGAGGAGC GGGCCCTGTG TCTCGTGGAT GTGAAGAAGG TGAAGCAGTC CCTCGCGCAG
    TCTCACCTCC CCGCCCAGCC AGACATCTCG CCCAACATCT TTGAAGCGGT CAAGGGCTGC
    CACTTGTTTG CTGCTGGCAA GATTGAGAGC GGGCTCTGCA TCTGTGCAGC CATGCCCAGC
    AAAGTTGTCA TTCTCCGCTA CAATGAAAAC CTCAGCAAGT ACTGCATTCG CAAAGAGATC
    GAGACCTCGG AGCCCTGCAG CTGTATCCAC TTCACCAACT ACAGTATCCT CATCGGAACC
    AATAAATTCT ACGAAATCGA CATGAAGCAG TACACGCTAG AGGAATTCCT GGATAAGAAC
    GACCATTCCT TGGCGCCTGC CGTGTTTGCC TCCTCTTCCA ACAGCTTCCC CGTGTCCATC
    ATGCAGGTGA ACGGCGCGGG GCAGCGGGAG GAGTTCCTGC TCTGTTTCCA CGAATTCGGG
    GTGTTCGTGG ATTCTTATGG AAGACGCAGC CGCACAGATG ATCTCAAGTG GAGTCGCTTA
    CCTCTGGCCT TTGCCTACAG AGAACCCTAT CTGTTTGTGA CTCACTTCAA CTCACTCGAA
    GTAATTGAGA TCCAGGCCCG CTCCTCTCTG GGGACCCCCG CCCGAGCATA TTTGGAAATC
    CCCAACCCAC GCTACCTGGG CCCTGCAATC TCCTCAGGAG CCATCTACCT GGCGTCCTCA
    TATCAGGATA AGTTAAGGGT CATCTGCTGC AAAGGAAACC TGGTGAAGGA GACCGGCACC
    GACCACCACC GTGGCCCATC CACCTCCCGC AGCCCCAACA AGCGAGGCCC GCCGACGTAC
    AACGAGCACA TCACCAAGCG CGTGGCCTCA AGCCCGGCGC CTCCCGAAGG CCCCAGCCAC
    CCTCGAGAGC CAAGCACACC CCACCGCTAC CGCGAGGGGC GGACCGAGCT GCGCAGGGAC
    AAGTCTCCGG GCCGCCCCCT GGAGCGCGAG AAGTCCCCCG GCCGGATGCT GAGCACGCGG
    AGGGAGCGCT CCCCCGGGCG GCTGTTTGAG GACAGCAGCA GGGGCCGGCT GCCCGTGGGG
    GCCGTGAGGA CCCCGCTGTC CCAGGTCAAC AAGGTCTGGG ACCAGTCTTC AGTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024833508.1
    CDS177..5693
    Translation

    Target ORF information:

    RefSeq Version XM_024977740.1
    Organism Bos taurus(cattle)
    Definition Bos taurus citron rho-interacting serine/threonine kinase (CIT), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024977740.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGAACTCAA ATAAGATGGT AAATGCCAAA CTCCCGGTTG GTACTCCAGA TTACATGGCC 
    CCTGAAGTGT TGACTGTGAT GAATGGGGAT GGAAAAGGTG CCTATAGCCT AGACTGTGAT
    TGGTGGTCAG TGGGAGTTAT TGCTTATGAG ATGGTTTATG GAAGATGCCC ATTCACTGAA
    GGAACCTCAG CCAAAACCTT CAATAACATC ATGAATTTCC AGCGGTTCTT GAAGTTTCCA
    GATGACCCCA AAGTTAGCAG TGAATTGCTT GATCTAATCC AGAGTTTGTT GTGTGGCCAG
    AAAGAGAGAC TGAAGTTTGA GGGCCTTTGC TGTCATCCTT TCTTCTCCAA AATTGACTGG
    ATTAACATTC GTAACTCTCC TCCCCCCTTC GTTCCCACCC TCAAGTCTGA TGATGACACC
    TCCAATTTTG ATGAACCAGA GAAGAATTCG TGGGTTTCAT CCTCTCCGTG CCAGCTGAAC
    CCCTCAGGTT TCTCGGGCGA AGAACTGCCG TTTGTGGGGT TTTCGTATAG CAAGGCACTG
    GGAATTCTTG GTAGATCTGA GCCTGTTGTA TCAAGTCTGG ACTCCCCTGC CAAGACTAGC
    TCCATGGAAA AGAAACTCCT TATCAAAAGC AAAGAGCTGC AGGACTCCCA GGACAAGTGT
    CACAAGATGG AGCAGGAAAT GACCCGGTTA CATCGGAGAG TGTCAGAGGT GGAGGCTGTG
    CTTAGTCAGA AGGAGGTGGA GCTGAAGGCC TCTGAGACTC AGAGATCCCT CCTGGAGCAG
    GACCTTGCTA CCTACATCAC AGAATGCAGT AGCTTAAAGC GAAGTTTGGA GCAAGCACGG
    ATGGAGGTGT CCCAAGAGGA CGACAAAGCA CTGCAGCTCC TCCATGATAT CAGAGAGCAG
    AGCCGGAAGC TCCAAGAAAT CAAAGAGCAG GAGTACCAGG CTCAAGTGGA AGAAATGAGG
    TTGATGATGA ATCAGTTGGA AGAGGACCTG GTTTCGGCGA GAAGGCGGAG TGACCTCTAT
    GAATCGGAGC TCAGAGAGTC TCGGCTGGCT GCTGAAGAGT TTAAGCGGAA AGCAACAGAA
    TGTCAGCATA AACTGATGAA GGCTAAAGAT CAAGGGAAGC CTGAAGTGGG AGAATATGCC
    AAACTGGAGA AGATCAATGC TGAGCAGCAG CTCAAAATTC AGGAGCTCCA AGAGAAGCTA
    GAAAAGGCTG TGAAAGCTAG CACAGAGGCC ACCGAGCTGC TGCAGAACAT CCGTCAGGCG
    AAGGAGCGGG CCGAGCGTGA GCTGGAGAAG CTGCACAACC GTGAGGACTC CTCCGAGGGC
    ATCAAGAAGA AGCTGGTGGA GGCCGAGGAA CGCCGCCACT CTCTGGAGAA CAAGGTAAAG
    AGGCTAGAGA CCATGGAGCG TAGAGAAAAC AGACTGAAGG ATGACATCCA GACAAAGTCC
    CAACAGATCC AGCAGATGGC TGATAAAATT CTGGAGCTGG AGGAGAAGCA CAGAGAAGCC
    CAGGTCTCAG CGCAGCATCT GGAGGTGCAC CTGAAACAGA AAGAGCAGCA CTATGAAGAA
    AAAATTAAAG TGTTGGACAA TCAGATAAAG AAAGACCTAG CAGACAAGGA GACACTGGAG
    AACCTGATGC AAAGACACGA AGAGGAGGCC CATGAGAAAG GCAAAATTCT CAGCGAGCAG
    AAGGCGATGA TCAATGCTAT GGATTCCAAG ATCAGATCCC TGGAACAAAG GATAGTGGAA
    TTGTCGGAAG CCAATAAACT TGCGGCCAAC AGCAGTCTCT TTACCCAGAG GAACATGAAG
    GCCCAGGAAG AGATGATTTC AGAACTCAGG CAACAGAAGT TTTACCTGGA GACGCAAGCT
    GGGAAATTGG AGGCCCAGAA CCGGAAGCTG GAGGAGCAGC TGGAAAAAAT AAGCCACCAA
    GACCACAGTG ACAAGAACCG TCTGCTGGAG CTGGAGACCA GGCTAAGGGA GGTCAGTCTA
    GAACATGAAG AGCAGAAACT GGAGCTCAAA CGCCAGCTCA CAGAGCTGCA GCTCTCCCTG
    CAGGAGCGTG AATCCCAGCT GACAGCCCTG CAGGCAGCAC GGGCGGCCCT GGAGAGCCAG
    CTCCGCCAGG CCAAGACGGA GCTGGAAGAG ACGACAGCAG AAGCAGAAGA AGAGATCCAG
    GCGCTTACGG CACATAGAGA TGAAATCCAG CGCAAATTCG ATGCCCTTCG TAACAGCTGT
    ACTGTGATCA CGGACTTGGA GGAGCAGCTA AACCAGCTGA CGGAGGACAA TGCCGAGCTC
    AACAACCAAA ACTTCTACTT GTCTAAACAA CTCGATGAGG CCTCTGGTGC CAACGACGAG
    ATTGTGCAGC TGCGAAGTGA AGTGGACCAT CTCCGCCGCG AGATCACTGA GAGGGAGATG
    CAGCTCACTA GCCAGAAGCA AACGATGGAG GCGCTGAAGA CCACGTGCAC AATGCTGGAG
    GAGCAGGTCA TGGACCTGGA GGCCCTGAAC GACGAGCTGC TGGAGAAGGA GCGGCAGTGG
    GAGGCCTGGA GGAGTGTCCT TGGTGACGAG AAGTCCCAGT TTGAGTGTCG GGTTCGAGAG
    TTGCAGAGGA TGCTGGACAC CGAAAAGCAG AGCAGGGCGA GGGCCGACCA GAGGATCACT
    GAGTCGCGCC AGGTGGTCGA GCTGGCAGTA AAGGAGCACA AGGCCGAGAT CCTGGCCTTG
    CAGCAGGCTC TCAAGGAGCA GAAGCTGAAG GCCGAGAGCC TCTCCGACAA GCTCAATGAT
    CTGGAGAAGA AGCACGCCAT GCTTGAAATG AATGCCCGAA GTTTACAACA GAAGCTGGAG
    ACAGAACGAG AGCTCAAACA AAGGCTTCTG GAAGAGCAAG CCAAGTTACA GCAGCAGATG
    GACCTGCAGA AGAATCATAT TTTCCGTCTG ACTCAAGGGC TGCAAGAAGC TCTGGATCGG
    GCTGATCTGC TGAAGACAGA AAGGAGTGAC CTGGAATATC AGCTGGAAAA CATTCAGGTT
    CTCTATTCTC ATGAGAAGGT GAAGATGGAA GGCACTATTT CCCAGCAAAC CAAACTCATC
    GATTTTCTGC AAGCCAAAAT GGACCAGCCT GCCAAAAAGA AAAAGGGTTT ATTTAGTCGA
    CGGAAAGAGG ACCCTGCTTT GCCCACACAG GTTCCTCTGC AGTACAATGA GCTGAAGGTG
    GCTCTGGAGA AGGAGAAAGC TCGCTGCGCG GAGCTCGAGG AAGCCCTGCA GAAGACCCGC
    ATCGAGCTCC GCTCCGCCCG GGAGGAAGCT GCCCACCGGA AAGCTGCAGA CCACCCACAC
    CCATCTACGC CGGCTACCGC CAGGCAGCAG ATCGCCATGT CCGCCATTGT GCGGTCACCC
    GAGCACCAGC CCAGCGCCCT GAGCCTGCTC GCCCCGCCGT CCAGCCGCAG GAAGGAGTCT
    TCCACTCCAG AGGAATTCAG CCGGCGTCTT AAGGAGCGCA TGCACCACAA CATCCCTCAC
    CGGTTCAACG TGGGACTGAA CATGCGAGCC ACGAAGTGCG CCGTGTGTCT GGATACCGTG
    CACTTCGGGC GCCAGGCATC CAAGTGTCTC GAATGTCAGG TCATGTGCCA CCCCAAGTGC
    TCCACATGCT TGCCAGCCAC CTGTGGCCTG CCAGCCGAAT ACGCCACACA CTTCACCGAG
    GCCTTCTGCC GCGACAAAAT GAACTCCCCG GGTCTGCAGT CCAAGGAGCC CAGCAGCAGC
    CTGCACCTGG AAGGGTGGAT GAAGGTGCCC AGGAATAACA AGCGAGGACA GCAAGGCTGG
    GACAGGAAGT ACATCGTTCT GGAGGGCTCC AAAGTCCTCA TTTATGACAA CGAAGCCAGA
    GAAGCTGGAC AGAGGCCGGT GGAAGAATTT GAGCTGTGCC TTCCCGACGG GGACGTGTCT
    ATTCATGGCG CCGTTGGTGC TTCTGAACTT GCAAACACAG CCAAAGCAGA TGTCCCCTAT
    GTCCTGAAGA TGGAGTCTCA CCCCCATACC ACCTGCTGGC CTGGGAGGAC CCTCTACTTG
    CTGGCTCCCA GCTTTCCCGA CAAGCAGCGC TGGGTCACCG CCTTAGAGTC AGTTGTGGCA
    GGTGGGAGAG TGTCTAGGGA AAAGGCGGAA GCCGACGCTG GCCGTGACTG TACTTCCTAC
    GAGCCTCTGC CCGCGTGGGC TCAGAAATTG CTCGGAAACT CCCTGTTGAA ACTGGAAGGT
    GATGACCGTC TGGACATGAA CTGCACGCTG CCCTTCAGCG ACCAGGTGGT GCTGGTGGGC
    ACCGAGGAAG GGCTGTATGC GCTGAATGTC TTGAAGAACT CCCTCACCCA CGTCCCAGGA
    ATCGGAGCAG TCTTCCAGAT TTACATCATC AAGGACCTGG AGAAGCTCCT CATGATCGCA
    GGAGAGGAGC GGGCCCTGTG TCTCGTGGAT GTGAAGAAGG TGAAGCAGTC CCTCGCGCAG
    TCTCACCTCC CCGCCCAGCC AGACATCTCG CCCAACATCT TTGAAGCGGT CAAGGGCTGC
    CACTTGTTTG CTGCTGGCAA GATTGAGAGC GGGCTCTGCA TCTGTGCAGC CATGCCCAGC
    AAAGTTGTCA TTCTCCGCTA CAATGAAAAC CTCAGCAAGT ACTGCATTCG CAAAGAGATC
    GAGACCTCGG AGCCCTGCAG CTGTATCCAC TTCACCAACT ACAGTATCCT CATCGGAACC
    AATAAATTCT ACGAAATCGA CATGAAGCAG TACACGCTAG AGGAATTCCT GGATAAGAAC
    GACCATTCCT TGGCGCCTGC CGTGTTTGCC TCCTCTTCCA ACAGCTTCCC CGTGTCCATC
    ATGCAGGTGA ACGGCGCGGG GCAGCGGGAG GAGTTCCTGC TCTGTTTCCA CGAATTCGGG
    GTGTTCGTGG ATTCTTATGG AAGACGCAGC CGCACAGATG ATCTCAAGTG GAGTCGCTTA
    CCTCTGGCCT TTGCCTACAG AGAACCCTAT CTGTTTGTGA CTCACTTCAA CTCACTCGAA
    GTAATTGAGA TCCAGGCCCG CTCCTCTCTG GGGACCCCCG CCCGAGCATA TTTGGAAATC
    CCCAACCCAC GCTACCTGGG CCCTGCAATC TCCTCAGGAG CCATCTACCT GGCGTCCTCA
    TATCAGGATA AGTTAAGGGT CATCTGCTGC AAAGGAAACC TGGTGAAGGA GACCGGCACC
    GACCACCACC GTGGCCCATC CACCTCCCGC AGCCCCAACA AGCGAGGCCC GCCGACGTAC
    AACGAGCACA TCACCAAGCG CGTGGCCTCA AGCCCGGCGC CTCCCGAAGG CCCCAGCCAC
    CCTCGAGAGC CAAGCACACC CCACCGCTAC CGCGAGGGGC GGACCGAGCT GCGCAGGGAC
    AAGTCTCCGG GCCGCCCCCT GGAGCGCGAG AAGTCCCCCG GCCGGATGCT GAGCACGCGG
    AGGGAGCGCT CCCCCGGGCG GCTGTTTGAG GACAGCAGCA GGGGCCGGCT GCCCGTGGGG
    GCCGTGAGGA CCCCGCTGTC CCAGGTCAAC AAGGTCTGGG ACCAGTCTTC AGTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa224629
    Clone ID Related Accession (Same CDS sequence) XM_024977741.1
    Accession Version XM_024977741.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5145bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product citron Rho-interacting kinase isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGTTGAAGT TCAAGTATGG AGCACGGAAT CTACTGGACG CTGGTACTGT TGAATCCATT 
    GCCAGCCGGG CCTCCAGGCT GAATCTTTTC TTCCAGGGGA AACCACCCTC CATGACTCAA
    CAGCAGATGT CTCTTCTTTC CCGAGAAGCG ATATTAGATG CCCTCTTTGT TCTCTTTAAA
    GAATGCAGTC AGCCAGCTCT GATGAAGATT AAGCACGTGA GCAACTTTGT CCAAAAGTAT
    TCCGATATCA TAGCTGAGTT GCAAGAGCTC CAGCCTTCAG TGAAGGACTT TGAAGTGAAA
    AGTGTCGTAG GCTGCGGTCA CTTTGCTGAA GTACAGGTGG TCAGAGAGAA AGCGACTGGG
    GATGTCTATG CCATGAAAGT CATGAAGAAG AAGGCCTTGT TGGCCCAGGA GCAGGTCTCA
    TATTTTGAGG AAGAACAGAA CATATTATCT CAGAGTACAA GTCCTTGGAT CCCCCAGTTG
    CAGTATGCCT TTCAGGACAA AAATAACCTT TATCTGGTCA TGGAATATCA GCCTGGAGGG
    GATTTGCTGT CACTTTTGAA TAGATATGAG GACCAATTAG ATGAAAATAT GATTCAGTTT
    TACCTAGCTG AACTGATTTT GGCTGTCCAC AGCGTTCATC AGATGGGCTA TGTACATCGA
    GACATCAAGC CCGAGAACAT CCTCATTGAC CGAACGGGAC ACATCAAGCT GGTGGATTTT
    GGATCAGCTG CTAAAATGAA CTCAAATAAG ATGGTAAATG CCAAACTCCC GGTTGGTACT
    CCAGATTACA TGGCCCCTGA AGTGTTGACT GTGATGAATG GGGATGGAAA AGGTGCCTAT
    AGCCTAGACT GTGATTGGTG GTCAGTGGGA GTTATTGCTT ATGAGATGGT TTATGGAAGA
    TGCCCATTCA CTGAAGGAAC CTCAGCCAAA ACCTTCAATA ACATCATGAA TTTCCAGCGG
    TTCTTGAAGT TTCCAGATGA CCCCAAAGTT AGCAGTGAAT TGCTTGATCT AATCCAGAGT
    TTGTTGTGTG GCCAGAAAGA GAGACTGAAG TTTGAGGGCC TTTGCTGTCA TCCTTTCTTC
    TCCAAAATTG ACTGGATTAA CATTCGTAAC TCTCCTCCCC CCTTCGTTCC CACCCTCAAG
    TCTGATGATG ACACCTCCAA TTTTGATGAA CCAGAGAAGA ATTCGTGGGT TTCATCCTCT
    CCGTGCCAGC TGAACCCCTC AGGTTTCTCG GGCGAAGAAC TGCCGTTTGT GGGGTTTTCG
    TATAGCAAGG CACTGGGAAT TCTTGGTAGA TCTGAGCCTG TTGTATCAAG TCTGGACTCC
    CCTGCCAAGA CTAGCTCCAT GGAAAAGAAA CTCCTTATCA AAAGCAAAGA GCTGCAGGAC
    TCCCAGGACA AGTGTCACAA GATGGAGCAG GAAATGACCC GGTTACATCG GAGAGTGTCA
    GAGGTGGAGG CTGTGCTTAG TCAGAAGGAG GTGGAGCTGA AGGCCTCTGA GACTCAGAGA
    TCCCTCCTGG AGCAGGACCT TGCTACCTAC ATCACAGAAT GCAGTAGCTT AAAGCGAAGT
    TTGGAGCAAG CACGGATGGA GGTGTCCCAA GAGGACGACA AAGCACTGCA GCTCCTCCAT
    GATATCAGAG AGCAGAGCCG GAAGCTCCAA GAAATCAAAG AGCAGGAGTA CCAGGCTCAA
    GTGGAAGAAA TGAGGTTGAT GATGAATCAG TTGGAAGAGG ACCTGGTTTC GGCGAGAAGG
    CGGAGTGACC TCTATGAATC GGAGCTCAGA GAGTCTCGGC TGGCTGCTGA AGAGTTTAAG
    CGGAAAGCAA CAGAATGTCA GCATAAACTG ATGAAGGCTA AAGATCAAGG GAAGCCTGAA
    GTGGGAGAAT ATGCCAAACT GGAGAAGATC AATGCTGAGC AGCAGCTCAA AATTCAGGAG
    CTCCAAGAGA AGCTAGAAAA GGCTGTGAAA GCTAGCACAG AGGCCACCGA GCTGCTGCAG
    AACATCCGTC AGGCGAAGGA GCGGGCCGAG CGTGAGCTGG AGAAGCTGCA CAACCGTGAG
    GACTCCTCCG AGGGCATCAA GAAGAAGCTG GTGGAGGCCG AGGAACGCCG CCACTCTCTG
    GAGAACAAGG TAAAGAGGCT AGAGACCATG GAGCGTAGAG AAAACAGACT GAAGGATGAC
    ATCCAGACAA AGTCCCAACA GATCCAGCAG ATGGCTGATA AAATTCTGGA GCTGGAGGAG
    AAGCACAGAG AAGCCCAGGT CTCAGCGCAG CATCTGGAGG TGCACCTGAA ACAGAAAGAG
    CAGCACTATG AAGAAAAAAT TAAAGTGTTG GACAATCAGA TAAAGAAAGA CCTAGCAGAC
    AAGGAGACAC TGGAGAACCT GATGCAAAGA CACGAAGAGG AGGCCCATGA GAAAGGCAAA
    ATTCTCAGCG AGCAGAAGGC GATGATCAAT GCTATGGATT CCAAGATCAG ATCCCTGGAA
    CAAAGGATAG TGGAATTGTC GGAAGCCAAT AAACTTGCGG CCAACAGCAG TCTCTTTACC
    CAGAGGAACA TGAAGGCCCA GGAAGAGATG ATTTCAGAAC TCAGGCAACA GAAGTTTTAC
    CTGGAGACGC AAGCTGGGAA ATTGGAGGCC CAGAACCGGA AGCTGGAGGA GCAGCTGGAA
    AAAATAAGCC ACCAAGACCA CAGTGACAAG AACCGTCTGC TGGAGCTGGA GACCAGGCTA
    AGGGAGGTCA GTCTAGAACA TGAAGAGCAG AAACTGGAGC TCAAACGCCA GCTCACAGAG
    CTGCAGCTCT CCCTGCAGGA GCGTGAATCC CAGCTGACAG CCCTGCAGGC AGCACGGGCG
    GCCCTGGAGA GCCAGCTCCG CCAGGCCAAG ACGGAGCTGG AAGAGACGAC AGCAGAAGCA
    GAAGAAGAGA TCCAGGCGCT TACGGCACAT AGAGATGAAA TCCAGCGCAA ATTCGATGCC
    CTTCGTAACA GCTGTACTGT GATCACGGAC TTGGAGGAGC AGCTAAACCA GCTGACGGAG
    GACAATGCCG AGCTCAACAA CCAAAACTTC TACTTGTCTA AACAACTCGA TGAGGCCTCT
    GGTGCCAACG ACGAGATTGT GCAGCTGCGA AGTGAAGTGG ACCATCTCCG CCGCGAGATC
    ACTGAGAGGG AGATGCAGCT CACTAGCCAG AAGCAAACGA TGGAGGCGCT GAAGACCACG
    TGCACAATGC TGGAGGAGCA GGTCATGGAC CTGGAGGCCC TGAACGACGA GCTGCTGGAG
    AAGGAGCGGC AGTGGGAGGC CTGGAGGAGT GTCCTTGGTG ACGAGAAGTC CCAGTTTGAG
    TGTCGGGTTC GAGAGTTGCA GAGGATGCTG GACACCGAAA AGCAGAGCAG GGCGAGGGCC
    GACCAGAGGA TCACTGAGTC GCGCCAGGTG GTCGAGCTGG CAGTAAAGGA GCACAAGGCC
    GAGATCCTGG CCTTGCAGCA GGCTCTCAAG GAGCAGAAGC TGAAGGCCGA GAGCCTCTCC
    GACAAGCTCA ATGATCTGGA GAAGAAGCAC GCCATGCTTG AAATGAATGC CCGAAGTTTA
    CAACAGAAGC TGGAGACAGA ACGAGAGCTC AAACAAAGGC TTCTGGAAGA GCAAGCCAAG
    TTACAGCAGC AGATGGACCT GCAGAAGAAT CATATTTTCC GTCTGACTCA AGGGCTGCAA
    GAAGCTCTGG ATCGGGCTGA TCTGCTGAAG ACAGAAAGGA GTGACCTGGA ATATCAGCTG
    GAAAACATTC AGGTTCTCTA TTCTCATGAG AAGGTGAAGA TGGAAGGCAC TATTTCCCAG
    CAAACCAAAC TCATCGATTT TCTGCAAGCC AAAATGGACC AGCCTGCCAA AAAGAAAAAG
    GGTTTATTTA GTCGACGGAA AGAGGACCCT GCTTTGCCCA CACAGGTTCC TCTGCAGTAC
    AATGAGCTGA AGGTGGCTCT GGAGAAGGAG AAAGCTCGCT GCGCGGAGCT CGAGGAAGCC
    CTGCAGAAGA CCCGCATCGA GCTCCGCTCC GCCCGGGAGG AAGCTGCCCA CCGGAAAGCT
    GCAGACCACC CACACCCATC TACGCCGGCT ACCGCCAGGC AGCAGATCGC CATGTCCGCC
    ATTGTGCGGT CACCCGAGCA CCAGCCCAGC GCCCTGAGCC TGCTCGCCCC GCCGTCCAGC
    CGCAGGAAGG AGTCTTCCAC TCCAGAGGAA TTCAGCCGGC GTCTTAAGGA GCGCATGCAC
    CACAACATCC CTCACCGGTT CAACGTGGGA CTGAACATGC GAGCCACGAA GTGCGCCGTG
    TGTCTGGATA CCGTGCACTT CGGGCGCCAG GCATCCAAGT GTCTCGAATG TCAGGTCATG
    TGCCACCCCA AGTGCTCCAC ATGCTTGCCA GCCACCTGTG GCCTGCCAGC CGAATACGCC
    ACACACTTCA CCGAGGCCTT CTGCCGCGAC AAAATGAACT CCCCGGGTCT GCAGTCCAAG
    GAGCCCAGCA GCAGCCTGCA CCTGGAAGGG TGGATGAAGG TGCCCAGGAA TAACAAGCGA
    GGACAGCAAG GCTGGGACAG GAAGTACATC GTTCTGGAGG GCTCCAAAGT CCTCATTTAT
    GACAACGAAG CCAGAGAAGC TGGACAGAGG CCGGTGGAAG AATTTGAGCT GTGCCTTCCC
    GACGGGGACG TGTCTATTCA TGGCGCCGTT GGTGCTTCTG AACTTGCAAA CACAGCCAAA
    GCAGATGTCC CCTATGTCCT GAAGATGGAG TCTCACCCCC ATACCACCTG CTGGCCTGGG
    AGGACCCTCT ACTTGCTGGC TCCCAGCTTT CCCGACAAGC AGCGCTGGGT CACCGCCTTA
    GAGTCAGTTG TGGCAGGTGG GAGAGTGTCT AGGGAAAAGG CGGAAGCCGA CGCTGGCCGT
    GACTGTACTT CCTACGAGCC TCTGCCCGCG TGGGCTCAGA AATTGCTCGG AAACTCCCTG
    TTGAAACTGG AAGGTGATGA CCGTCTGGAC ATGAACTGCA CGCTGCCCTT CAGCGACCAG
    GTGGTGCTGG TGGGCACCGA GGAAGGGCTG TATGCGCTGA ATGTCTTGAA GAACTCCCTC
    ACCCACGTCC CAGGAATCGG AGCAGTCTTC CAGATTTACA TCATCAAGGA CCTGGAGAAG
    CTCCTCATGA TCGCAGATCT AACACTTGGA CCTAAACTGT GTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024833509.1
    CDS113..5257
    Translation

    Target ORF information:

    RefSeq Version XM_024977741.1
    Organism Bos taurus(cattle)
    Definition Bos taurus citron rho-interacting serine/threonine kinase (CIT), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024977741.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGTTGAAGT TCAAGTATGG AGCACGGAAT CTACTGGACG CTGGTACTGT TGAATCCATT 
    GCCAGCCGGG CCTCCAGGCT GAATCTTTTC TTCCAGGGGA AACCACCCTC CATGACTCAA
    CAGCAGATGT CTCTTCTTTC CCGAGAAGCG ATATTAGATG CCCTCTTTGT TCTCTTTAAA
    GAATGCAGTC AGCCAGCTCT GATGAAGATT AAGCACGTGA GCAACTTTGT CCAAAAGTAT
    TCCGATATCA TAGCTGAGTT GCAAGAGCTC CAGCCTTCAG TGAAGGACTT TGAAGTGAAA
    AGTGTCGTAG GCTGCGGTCA CTTTGCTGAA GTACAGGTGG TCAGAGAGAA AGCGACTGGG
    GATGTCTATG CCATGAAAGT CATGAAGAAG AAGGCCTTGT TGGCCCAGGA GCAGGTCTCA
    TATTTTGAGG AAGAACAGAA CATATTATCT CAGAGTACAA GTCCTTGGAT CCCCCAGTTG
    CAGTATGCCT TTCAGGACAA AAATAACCTT TATCTGGTCA TGGAATATCA GCCTGGAGGG
    GATTTGCTGT CACTTTTGAA TAGATATGAG GACCAATTAG ATGAAAATAT GATTCAGTTT
    TACCTAGCTG AACTGATTTT GGCTGTCCAC AGCGTTCATC AGATGGGCTA TGTACATCGA
    GACATCAAGC CCGAGAACAT CCTCATTGAC CGAACGGGAC ACATCAAGCT GGTGGATTTT
    GGATCAGCTG CTAAAATGAA CTCAAATAAG ATGGTAAATG CCAAACTCCC GGTTGGTACT
    CCAGATTACA TGGCCCCTGA AGTGTTGACT GTGATGAATG GGGATGGAAA AGGTGCCTAT
    AGCCTAGACT GTGATTGGTG GTCAGTGGGA GTTATTGCTT ATGAGATGGT TTATGGAAGA
    TGCCCATTCA CTGAAGGAAC CTCAGCCAAA ACCTTCAATA ACATCATGAA TTTCCAGCGG
    TTCTTGAAGT TTCCAGATGA CCCCAAAGTT AGCAGTGAAT TGCTTGATCT AATCCAGAGT
    TTGTTGTGTG GCCAGAAAGA GAGACTGAAG TTTGAGGGCC TTTGCTGTCA TCCTTTCTTC
    TCCAAAATTG ACTGGATTAA CATTCGTAAC TCTCCTCCCC CCTTCGTTCC CACCCTCAAG
    TCTGATGATG ACACCTCCAA TTTTGATGAA CCAGAGAAGA ATTCGTGGGT TTCATCCTCT
    CCGTGCCAGC TGAACCCCTC AGGTTTCTCG GGCGAAGAAC TGCCGTTTGT GGGGTTTTCG
    TATAGCAAGG CACTGGGAAT TCTTGGTAGA TCTGAGCCTG TTGTATCAAG TCTGGACTCC
    CCTGCCAAGA CTAGCTCCAT GGAAAAGAAA CTCCTTATCA AAAGCAAAGA GCTGCAGGAC
    TCCCAGGACA AGTGTCACAA GATGGAGCAG GAAATGACCC GGTTACATCG GAGAGTGTCA
    GAGGTGGAGG CTGTGCTTAG TCAGAAGGAG GTGGAGCTGA AGGCCTCTGA GACTCAGAGA
    TCCCTCCTGG AGCAGGACCT TGCTACCTAC ATCACAGAAT GCAGTAGCTT AAAGCGAAGT
    TTGGAGCAAG CACGGATGGA GGTGTCCCAA GAGGACGACA AAGCACTGCA GCTCCTCCAT
    GATATCAGAG AGCAGAGCCG GAAGCTCCAA GAAATCAAAG AGCAGGAGTA CCAGGCTCAA
    GTGGAAGAAA TGAGGTTGAT GATGAATCAG TTGGAAGAGG ACCTGGTTTC GGCGAGAAGG
    CGGAGTGACC TCTATGAATC GGAGCTCAGA GAGTCTCGGC TGGCTGCTGA AGAGTTTAAG
    CGGAAAGCAA CAGAATGTCA GCATAAACTG ATGAAGGCTA AAGATCAAGG GAAGCCTGAA
    GTGGGAGAAT ATGCCAAACT GGAGAAGATC AATGCTGAGC AGCAGCTCAA AATTCAGGAG
    CTCCAAGAGA AGCTAGAAAA GGCTGTGAAA GCTAGCACAG AGGCCACCGA GCTGCTGCAG
    AACATCCGTC AGGCGAAGGA GCGGGCCGAG CGTGAGCTGG AGAAGCTGCA CAACCGTGAG
    GACTCCTCCG AGGGCATCAA GAAGAAGCTG GTGGAGGCCG AGGAACGCCG CCACTCTCTG
    GAGAACAAGG TAAAGAGGCT AGAGACCATG GAGCGTAGAG AAAACAGACT GAAGGATGAC
    ATCCAGACAA AGTCCCAACA GATCCAGCAG ATGGCTGATA AAATTCTGGA GCTGGAGGAG
    AAGCACAGAG AAGCCCAGGT CTCAGCGCAG CATCTGGAGG TGCACCTGAA ACAGAAAGAG
    CAGCACTATG AAGAAAAAAT TAAAGTGTTG GACAATCAGA TAAAGAAAGA CCTAGCAGAC
    AAGGAGACAC TGGAGAACCT GATGCAAAGA CACGAAGAGG AGGCCCATGA GAAAGGCAAA
    ATTCTCAGCG AGCAGAAGGC GATGATCAAT GCTATGGATT CCAAGATCAG ATCCCTGGAA
    CAAAGGATAG TGGAATTGTC GGAAGCCAAT AAACTTGCGG CCAACAGCAG TCTCTTTACC
    CAGAGGAACA TGAAGGCCCA GGAAGAGATG ATTTCAGAAC TCAGGCAACA GAAGTTTTAC
    CTGGAGACGC AAGCTGGGAA ATTGGAGGCC CAGAACCGGA AGCTGGAGGA GCAGCTGGAA
    AAAATAAGCC ACCAAGACCA CAGTGACAAG AACCGTCTGC TGGAGCTGGA GACCAGGCTA
    AGGGAGGTCA GTCTAGAACA TGAAGAGCAG AAACTGGAGC TCAAACGCCA GCTCACAGAG
    CTGCAGCTCT CCCTGCAGGA GCGTGAATCC CAGCTGACAG CCCTGCAGGC AGCACGGGCG
    GCCCTGGAGA GCCAGCTCCG CCAGGCCAAG ACGGAGCTGG AAGAGACGAC AGCAGAAGCA
    GAAGAAGAGA TCCAGGCGCT TACGGCACAT AGAGATGAAA TCCAGCGCAA ATTCGATGCC
    CTTCGTAACA GCTGTACTGT GATCACGGAC TTGGAGGAGC AGCTAAACCA GCTGACGGAG
    GACAATGCCG AGCTCAACAA CCAAAACTTC TACTTGTCTA AACAACTCGA TGAGGCCTCT
    GGTGCCAACG ACGAGATTGT GCAGCTGCGA AGTGAAGTGG ACCATCTCCG CCGCGAGATC
    ACTGAGAGGG AGATGCAGCT CACTAGCCAG AAGCAAACGA TGGAGGCGCT GAAGACCACG
    TGCACAATGC TGGAGGAGCA GGTCATGGAC CTGGAGGCCC TGAACGACGA GCTGCTGGAG
    AAGGAGCGGC AGTGGGAGGC CTGGAGGAGT GTCCTTGGTG ACGAGAAGTC CCAGTTTGAG
    TGTCGGGTTC GAGAGTTGCA GAGGATGCTG GACACCGAAA AGCAGAGCAG GGCGAGGGCC
    GACCAGAGGA TCACTGAGTC GCGCCAGGTG GTCGAGCTGG CAGTAAAGGA GCACAAGGCC
    GAGATCCTGG CCTTGCAGCA GGCTCTCAAG GAGCAGAAGC TGAAGGCCGA GAGCCTCTCC
    GACAAGCTCA ATGATCTGGA GAAGAAGCAC GCCATGCTTG AAATGAATGC CCGAAGTTTA
    CAACAGAAGC TGGAGACAGA ACGAGAGCTC AAACAAAGGC TTCTGGAAGA GCAAGCCAAG
    TTACAGCAGC AGATGGACCT GCAGAAGAAT CATATTTTCC GTCTGACTCA AGGGCTGCAA
    GAAGCTCTGG ATCGGGCTGA TCTGCTGAAG ACAGAAAGGA GTGACCTGGA ATATCAGCTG
    GAAAACATTC AGGTTCTCTA TTCTCATGAG AAGGTGAAGA TGGAAGGCAC TATTTCCCAG
    CAAACCAAAC TCATCGATTT TCTGCAAGCC AAAATGGACC AGCCTGCCAA AAAGAAAAAG
    GGTTTATTTA GTCGACGGAA AGAGGACCCT GCTTTGCCCA CACAGGTTCC TCTGCAGTAC
    AATGAGCTGA AGGTGGCTCT GGAGAAGGAG AAAGCTCGCT GCGCGGAGCT CGAGGAAGCC
    CTGCAGAAGA CCCGCATCGA GCTCCGCTCC GCCCGGGAGG AAGCTGCCCA CCGGAAAGCT
    GCAGACCACC CACACCCATC TACGCCGGCT ACCGCCAGGC AGCAGATCGC CATGTCCGCC
    ATTGTGCGGT CACCCGAGCA CCAGCCCAGC GCCCTGAGCC TGCTCGCCCC GCCGTCCAGC
    CGCAGGAAGG AGTCTTCCAC TCCAGAGGAA TTCAGCCGGC GTCTTAAGGA GCGCATGCAC
    CACAACATCC CTCACCGGTT CAACGTGGGA CTGAACATGC GAGCCACGAA GTGCGCCGTG
    TGTCTGGATA CCGTGCACTT CGGGCGCCAG GCATCCAAGT GTCTCGAATG TCAGGTCATG
    TGCCACCCCA AGTGCTCCAC ATGCTTGCCA GCCACCTGTG GCCTGCCAGC CGAATACGCC
    ACACACTTCA CCGAGGCCTT CTGCCGCGAC AAAATGAACT CCCCGGGTCT GCAGTCCAAG
    GAGCCCAGCA GCAGCCTGCA CCTGGAAGGG TGGATGAAGG TGCCCAGGAA TAACAAGCGA
    GGACAGCAAG GCTGGGACAG GAAGTACATC GTTCTGGAGG GCTCCAAAGT CCTCATTTAT
    GACAACGAAG CCAGAGAAGC TGGACAGAGG CCGGTGGAAG AATTTGAGCT GTGCCTTCCC
    GACGGGGACG TGTCTATTCA TGGCGCCGTT GGTGCTTCTG AACTTGCAAA CACAGCCAAA
    GCAGATGTCC CCTATGTCCT GAAGATGGAG TCTCACCCCC ATACCACCTG CTGGCCTGGG
    AGGACCCTCT ACTTGCTGGC TCCCAGCTTT CCCGACAAGC AGCGCTGGGT CACCGCCTTA
    GAGTCAGTTG TGGCAGGTGG GAGAGTGTCT AGGGAAAAGG CGGAAGCCGA CGCTGGCCGT
    GACTGTACTT CCTACGAGCC TCTGCCCGCG TGGGCTCAGA AATTGCTCGG AAACTCCCTG
    TTGAAACTGG AAGGTGATGA CCGTCTGGAC ATGAACTGCA CGCTGCCCTT CAGCGACCAG
    GTGGTGCTGG TGGGCACCGA GGAAGGGCTG TATGCGCTGA ATGTCTTGAA GAACTCCCTC
    ACCCACGTCC CAGGAATCGG AGCAGTCTTC CAGATTTACA TCATCAAGGA CCTGGAGAAG
    CTCCTCATGA TCGCAGATCT AACACTTGGA CCTAAACTGT GTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa224630
    Clone ID Related Accession (Same CDS sequence) XM_024977743.1 , XM_024977742.1
    Accession Version XM_024977743.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4914bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product citron Rho-interacting kinase isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGAAAAGA AACTCCTTAT CAAAAGCAAA GAGCTGCAGG ACTCCCAGGA CAAGTGTCAC 
    AAGATGGAGC AGGAAATGAC CCGGTTACAT CGGAGAGTGT CAGAGGTGGA GGCTGTGCTT
    AGTCAGAAGG AGGTGGAGCT GAAGGCCTCT GAGACTCAGA GATCCCTCCT GGAGCAGGAC
    CTTGCTACCT ACATCACAGA ATGCAGTAGC TTAAAGCGAA GTTTGGAGCA AGCACGGATG
    GAGGTGTCCC AAGAGGACGA CAAAGCACTG CAGCTCCTCC ATGATATCAG AGAGCAGAGC
    CGGAAGCTCC AAGAAATCAA AGAGCAGGAG TACCAGGCTC AAGTGGAAGA AATGAGGTTG
    ATGATGAATC AGTTGGAAGA GGACCTGGTT TCGGCGAGAA GGCGGAGTGA CCTCTATGAA
    TCGGAGCTCA GAGAGTCTCG GCTGGCTGCT GAAGAGTTTA AGCGGAAAGC AACAGAATGT
    CAGCATAAAC TGATGAAGGC TAAAGATCAA GGGAAGCCTG AAGTGGGAGA ATATGCCAAA
    CTGGAGAAGA TCAATGCTGA GCAGCAGCTC AAAATTCAGG AGCTCCAAGA GAAGCTAGAA
    AAGGCTGTGA AAGCTAGCAC AGAGGCCACC GAGCTGCTGC AGAACATCCG TCAGGCGAAG
    GAGCGGGCCG AGCGTGAGCT GGAGAAGCTG CACAACCGTG AGGACTCCTC CGAGGGCATC
    AAGAAGAAGC TGGTGGAGGC CGAGGAACGC CGCCACTCTC TGGAGAACAA GGTAAAGAGG
    CTAGAGACCA TGGAGCGTAG AGAAAACAGA CTGAAGGATG ACATCCAGAC AAAGTCCCAA
    CAGATCCAGC AGATGGCTGA TAAAATTCTG GAGCTGGAGG AGAAGCACAG AGAAGCCCAG
    GTCTCAGCGC AGCATCTGGA GGTGCACCTG AAACAGAAAG AGCAGCACTA TGAAGAAAAA
    ATTAAAGTGT TGGACAATCA GATAAAGAAA GACCTAGCAG ACAAGGAGAC ACTGGAGAAC
    CTGATGCAAA GACACGAAGA GGAGGCCCAT GAGAAAGGCA AAATTCTCAG CGAGCAGAAG
    GCGATGATCA ATGCTATGGA TTCCAAGATC AGATCCCTGG AACAAAGGAT AGTGGAATTG
    TCGGAAGCCA ATAAACTTGC GGCCAACAGC AGTCTCTTTA CCCAGAGGAA CATGAAGGCC
    CAGGAAGAGA TGATTTCAGA ACTCAGGCAA CAGAAGTTTT ACCTGGAGAC GCAAGCTGGG
    AAATTGGAGG CCCAGAACCG GAAGCTGGAG GAGCAGCTGG AAAAAATAAG CCACCAAGAC
    CACAGTGACA AGAACCGTCT GCTGGAGCTG GAGACCAGGC TAAGGGAGGT CAGTCTAGAA
    CATGAAGAGC AGAAACTGGA GCTCAAACGC CAGCTCACAG AGCTGCAGCT CTCCCTGCAG
    GAGCGTGAAT CCCAGCTGAC AGCCCTGCAG GCAGCACGGG CGGCCCTGGA GAGCCAGCTC
    CGCCAGGCCA AGACGGAGCT GGAAGAGACG ACAGCAGAAG CAGAAGAAGA GATCCAGGCG
    CTTACGGCAC ATAGAGATGA AATCCAGCGC AAATTCGATG CCCTTCGTAA CAGCTGTACT
    GTGATCACGG ACTTGGAGGA GCAGCTAAAC CAGCTGACGG AGGACAATGC CGAGCTCAAC
    AACCAAAACT TCTACTTGTC TAAACAACTC GATGAGGCCT CTGGTGCCAA CGACGAGATT
    GTGCAGCTGC GAAGTGAAGT GGACCATCTC CGCCGCGAGA TCACTGAGAG GGAGATGCAG
    CTCACTAGCC AGAAGCAAAC GATGGAGGCG CTGAAGACCA CGTGCACAAT GCTGGAGGAG
    CAGGTCATGG ACCTGGAGGC CCTGAACGAC GAGCTGCTGG AGAAGGAGCG GCAGTGGGAG
    GCCTGGAGGA GTGTCCTTGG TGACGAGAAG TCCCAGTTTG AGTGTCGGGT TCGAGAGTTG
    CAGAGGATGC TGGACACCGA AAAGCAGAGC AGGGCGAGGG CCGACCAGAG GATCACTGAG
    TCGCGCCAGG TGGTCGAGCT GGCAGTAAAG GAGCACAAGG CCGAGATCCT GGCCTTGCAG
    CAGGCTCTCA AGGAGCAGAA GCTGAAGGCC GAGAGCCTCT CCGACAAGCT CAATGATCTG
    GAGAAGAAGC ACGCCATGCT TGAAATGAAT GCCCGAAGTT TACAACAGAA GCTGGAGACA
    GAACGAGAGC TCAAACAAAG GCTTCTGGAA GAGCAAGCCA AGTTACAGCA GCAGATGGAC
    CTGCAGAAGA ATCATATTTT CCGTCTGACT CAAGGGCTGC AAGAAGCTCT GGATCGGGCT
    GATCTGCTGA AGACAGAAAG GAGTGACCTG GAATATCAGC TGGAAAACAT TCAGGTTCTC
    TATTCTCATG AGAAGGTGAA GATGGAAGGC ACTATTTCCC AGCAAACCAA ACTCATCGAT
    TTTCTGCAAG CCAAAATGGA CCAGCCTGCC AAAAAGAAAA AGGGTTTATT TAGTCGACGG
    AAAGAGGACC CTGCTTTGCC CACACAGGTT CCTCTGCAGT ACAATGAGCT GAAGGTGGCT
    CTGGAGAAGG AGAAAGCTCG CTGCGCGGAG CTCGAGGAAG CCCTGCAGAA GACCCGCATC
    GAGCTCCGCT CCGCCCGGGA GGAAGCTGCC CACCGGAAAG CTGCAGACCA CCCACACCCA
    TCTACGCCGG CTACCGCCAG GCAGCAGATC GCCATGTCCG CCATTGTGCG GTCACCCGAG
    CACCAGCCCA GCGCCCTGAG CCTGCTCGCC CCGCCGTCCA GCCGCAGGAA GGAGTCTTCC
    ACTCCAGAGG AATTCAGCCG GCGTCTTAAG GAGCGCATGC ACCACAACAT CCCTCACCGG
    TTCAACGTGG GACTGAACAT GCGAGCCACG AAGTGCGCCG TGTGTCTGGA TACCGTGCAC
    TTCGGGCGCC AGGCATCCAA GTGTCTCGAA TGTCAGGTCA TGTGCCACCC CAAGTGCTCC
    ACATGCTTGC CAGCCACCTG TGGCCTGCCA GCCGAATACG CCACACACTT CACCGAGGCC
    TTCTGCCGCG ACAAAATGAA CTCCCCGGGT CTGCAGTCCA AGGAGCCCAG CAGCAGCCTG
    CACCTGGAAG GGTGGATGAA GGTGCCCAGG AATAACAAGC GAGGACAGCA AGGCTGGGAC
    AGGAAGTACA TCGTTCTGGA GGGCTCCAAA GTCCTCATTT ATGACAACGA AGCCAGAGAA
    GCTGGACAGA GGCCGGTGGA AGAATTTGAG CTGTGCCTTC CCGACGGGGA CGTGTCTATT
    CATGGCGCCG TTGGTGCTTC TGAACTTGCA AACACAGCCA AAGCAGATGT CCCCTATGTC
    CTGAAGATGG AGTCTCACCC CCATACCACC TGCTGGCCTG GGAGGACCCT CTACTTGCTG
    GCTCCCAGCT TTCCCGACAA GCAGCGCTGG GTCACCGCCT TAGAGTCAGT TGTGGCAGGT
    GGGAGAGTGT CTAGGGAAAA GGCGGAAGCC GACGCTGGCC GTGACTGTAC TTCCTACGAG
    CCTCTGCCCG CGTGGGCTCA GAAATTGCTC GGAAACTCCC TGTTGAAACT GGAAGGTGAT
    GACCGTCTGG ACATGAACTG CACGCTGCCC TTCAGCGACC AGGTGGTGCT GGTGGGCACC
    GAGGAAGGGC TGTATGCGCT GAATGTCTTG AAGAACTCCC TCACCCACGT CCCAGGAATC
    GGAGCAGTCT TCCAGATTTA CATCATCAAG GACCTGGAGA AGCTCCTCAT GATCGCAGGA
    GAGGAGCGGG CCCTGTGTCT CGTGGATGTG AAGAAGGTGA AGCAGTCCCT CGCGCAGTCT
    CACCTCCCCG CCCAGCCAGA CATCTCGCCC AACATCTTTG AAGCGGTCAA GGGCTGCCAC
    TTGTTTGCTG CTGGCAAGAT TGAGAGCGGG CTCTGCATCT GTGCAGCCAT GCCCAGCAAA
    GTTGTCATTC TCCGCTACAA TGAAAACCTC AGCAAGTACT GCATTCGCAA AGAGATCGAG
    ACCTCGGAGC CCTGCAGCTG TATCCACTTC ACCAACTACA GTATCCTCAT CGGAACCAAT
    AAATTCTACG AAATCGACAT GAAGCAGTAC ACGCTAGAGG AATTCCTGGA TAAGAACGAC
    CATTCCTTGG CGCCTGCCGT GTTTGCCTCC TCTTCCAACA GCTTCCCCGT GTCCATCATG
    CAGGTGAACG GCGCGGGGCA GCGGGAGGAG TTCCTGCTCT GTTTCCACGA ATTCGGGGTG
    TTCGTGGATT CTTATGGAAG ACGCAGCCGC ACAGATGATC TCAAGTGGAG TCGCTTACCT
    CTGGCCTTTG CCTACAGAGA ACCCTATCTG TTTGTGACTC ACTTCAACTC ACTCGAAGTA
    ATTGAGATCC AGGCCCGCTC CTCTCTGGGG ACCCCCGCCC GAGCATATTT GGAAATCCCC
    AACCCACGCT ACCTGGGCCC TGCAATCTCC TCAGGAGCCA TCTACCTGGC GTCCTCATAT
    CAGGATAAGT TAAGGGTCAT CTGCTGCAAA GGAAACCTGG TGAAGGAGAC CGGCACCGAC
    CACCACCGTG GCCCATCCAC CTCCCGCAGC CCCAACAAGC GAGGCCCGCC GACGTACAAC
    GAGCACATCA CCAAGCGCGT GGCCTCAAGC CCGGCGCCTC CCGAAGGCCC CAGCCACCCT
    CGAGAGCCAA GCACACCCCA CCGCTACCGC GAGGGGCGGA CCGAGCTGCG CAGGGACAAG
    TCTCCGGGCC GCCCCCTGGA GCGCGAGAAG TCCCCCGGCC GGATGCTGAG CACGCGGAGG
    GAGCGCTCCC CCGGGCGGCT GTTTGAGGAC AGCAGCAGGG GCCGGCTGCC CGTGGGGGCC
    GTGAGGACCC CGCTGTCCCA GGTCAACAAG GTCTGGGACC AGTCTTCAGT ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024833511.1
    CDS173..5086
    Translation

    Target ORF information:

    RefSeq Version XM_024977743.1
    Organism Bos taurus(cattle)
    Definition Bos taurus citron rho-interacting serine/threonine kinase (CIT), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024977743.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGAAAAGA AACTCCTTAT CAAAAGCAAA GAGCTGCAGG ACTCCCAGGA CAAGTGTCAC 
    AAGATGGAGC AGGAAATGAC CCGGTTACAT CGGAGAGTGT CAGAGGTGGA GGCTGTGCTT
    AGTCAGAAGG AGGTGGAGCT GAAGGCCTCT GAGACTCAGA GATCCCTCCT GGAGCAGGAC
    CTTGCTACCT ACATCACAGA ATGCAGTAGC TTAAAGCGAA GTTTGGAGCA AGCACGGATG
    GAGGTGTCCC AAGAGGACGA CAAAGCACTG CAGCTCCTCC ATGATATCAG AGAGCAGAGC
    CGGAAGCTCC AAGAAATCAA AGAGCAGGAG TACCAGGCTC AAGTGGAAGA AATGAGGTTG
    ATGATGAATC AGTTGGAAGA GGACCTGGTT TCGGCGAGAA GGCGGAGTGA CCTCTATGAA
    TCGGAGCTCA GAGAGTCTCG GCTGGCTGCT GAAGAGTTTA AGCGGAAAGC AACAGAATGT
    CAGCATAAAC TGATGAAGGC TAAAGATCAA GGGAAGCCTG AAGTGGGAGA ATATGCCAAA
    CTGGAGAAGA TCAATGCTGA GCAGCAGCTC AAAATTCAGG AGCTCCAAGA GAAGCTAGAA
    AAGGCTGTGA AAGCTAGCAC AGAGGCCACC GAGCTGCTGC AGAACATCCG TCAGGCGAAG
    GAGCGGGCCG AGCGTGAGCT GGAGAAGCTG CACAACCGTG AGGACTCCTC CGAGGGCATC
    AAGAAGAAGC TGGTGGAGGC CGAGGAACGC CGCCACTCTC TGGAGAACAA GGTAAAGAGG
    CTAGAGACCA TGGAGCGTAG AGAAAACAGA CTGAAGGATG ACATCCAGAC AAAGTCCCAA
    CAGATCCAGC AGATGGCTGA TAAAATTCTG GAGCTGGAGG AGAAGCACAG AGAAGCCCAG
    GTCTCAGCGC AGCATCTGGA GGTGCACCTG AAACAGAAAG AGCAGCACTA TGAAGAAAAA
    ATTAAAGTGT TGGACAATCA GATAAAGAAA GACCTAGCAG ACAAGGAGAC ACTGGAGAAC
    CTGATGCAAA GACACGAAGA GGAGGCCCAT GAGAAAGGCA AAATTCTCAG CGAGCAGAAG
    GCGATGATCA ATGCTATGGA TTCCAAGATC AGATCCCTGG AACAAAGGAT AGTGGAATTG
    TCGGAAGCCA ATAAACTTGC GGCCAACAGC AGTCTCTTTA CCCAGAGGAA CATGAAGGCC
    CAGGAAGAGA TGATTTCAGA ACTCAGGCAA CAGAAGTTTT ACCTGGAGAC GCAAGCTGGG
    AAATTGGAGG CCCAGAACCG GAAGCTGGAG GAGCAGCTGG AAAAAATAAG CCACCAAGAC
    CACAGTGACA AGAACCGTCT GCTGGAGCTG GAGACCAGGC TAAGGGAGGT CAGTCTAGAA
    CATGAAGAGC AGAAACTGGA GCTCAAACGC CAGCTCACAG AGCTGCAGCT CTCCCTGCAG
    GAGCGTGAAT CCCAGCTGAC AGCCCTGCAG GCAGCACGGG CGGCCCTGGA GAGCCAGCTC
    CGCCAGGCCA AGACGGAGCT GGAAGAGACG ACAGCAGAAG CAGAAGAAGA GATCCAGGCG
    CTTACGGCAC ATAGAGATGA AATCCAGCGC AAATTCGATG CCCTTCGTAA CAGCTGTACT
    GTGATCACGG ACTTGGAGGA GCAGCTAAAC CAGCTGACGG AGGACAATGC CGAGCTCAAC
    AACCAAAACT TCTACTTGTC TAAACAACTC GATGAGGCCT CTGGTGCCAA CGACGAGATT
    GTGCAGCTGC GAAGTGAAGT GGACCATCTC CGCCGCGAGA TCACTGAGAG GGAGATGCAG
    CTCACTAGCC AGAAGCAAAC GATGGAGGCG CTGAAGACCA CGTGCACAAT GCTGGAGGAG
    CAGGTCATGG ACCTGGAGGC CCTGAACGAC GAGCTGCTGG AGAAGGAGCG GCAGTGGGAG
    GCCTGGAGGA GTGTCCTTGG TGACGAGAAG TCCCAGTTTG AGTGTCGGGT TCGAGAGTTG
    CAGAGGATGC TGGACACCGA AAAGCAGAGC AGGGCGAGGG CCGACCAGAG GATCACTGAG
    TCGCGCCAGG TGGTCGAGCT GGCAGTAAAG GAGCACAAGG CCGAGATCCT GGCCTTGCAG
    CAGGCTCTCA AGGAGCAGAA GCTGAAGGCC GAGAGCCTCT CCGACAAGCT CAATGATCTG
    GAGAAGAAGC ACGCCATGCT TGAAATGAAT GCCCGAAGTT TACAACAGAA GCTGGAGACA
    GAACGAGAGC TCAAACAAAG GCTTCTGGAA GAGCAAGCCA AGTTACAGCA GCAGATGGAC
    CTGCAGAAGA ATCATATTTT CCGTCTGACT CAAGGGCTGC AAGAAGCTCT GGATCGGGCT
    GATCTGCTGA AGACAGAAAG GAGTGACCTG GAATATCAGC TGGAAAACAT TCAGGTTCTC
    TATTCTCATG AGAAGGTGAA GATGGAAGGC ACTATTTCCC AGCAAACCAA ACTCATCGAT
    TTTCTGCAAG CCAAAATGGA CCAGCCTGCC AAAAAGAAAA AGGGTTTATT TAGTCGACGG
    AAAGAGGACC CTGCTTTGCC CACACAGGTT CCTCTGCAGT ACAATGAGCT GAAGGTGGCT
    CTGGAGAAGG AGAAAGCTCG CTGCGCGGAG CTCGAGGAAG CCCTGCAGAA GACCCGCATC
    GAGCTCCGCT CCGCCCGGGA GGAAGCTGCC CACCGGAAAG CTGCAGACCA CCCACACCCA
    TCTACGCCGG CTACCGCCAG GCAGCAGATC GCCATGTCCG CCATTGTGCG GTCACCCGAG
    CACCAGCCCA GCGCCCTGAG CCTGCTCGCC CCGCCGTCCA GCCGCAGGAA GGAGTCTTCC
    ACTCCAGAGG AATTCAGCCG GCGTCTTAAG GAGCGCATGC ACCACAACAT CCCTCACCGG
    TTCAACGTGG GACTGAACAT GCGAGCCACG AAGTGCGCCG TGTGTCTGGA TACCGTGCAC
    TTCGGGCGCC AGGCATCCAA GTGTCTCGAA TGTCAGGTCA TGTGCCACCC CAAGTGCTCC
    ACATGCTTGC CAGCCACCTG TGGCCTGCCA GCCGAATACG CCACACACTT CACCGAGGCC
    TTCTGCCGCG ACAAAATGAA CTCCCCGGGT CTGCAGTCCA AGGAGCCCAG CAGCAGCCTG
    CACCTGGAAG GGTGGATGAA GGTGCCCAGG AATAACAAGC GAGGACAGCA AGGCTGGGAC
    AGGAAGTACA TCGTTCTGGA GGGCTCCAAA GTCCTCATTT ATGACAACGA AGCCAGAGAA
    GCTGGACAGA GGCCGGTGGA AGAATTTGAG CTGTGCCTTC CCGACGGGGA CGTGTCTATT
    CATGGCGCCG TTGGTGCTTC TGAACTTGCA AACACAGCCA AAGCAGATGT CCCCTATGTC
    CTGAAGATGG AGTCTCACCC CCATACCACC TGCTGGCCTG GGAGGACCCT CTACTTGCTG
    GCTCCCAGCT TTCCCGACAA GCAGCGCTGG GTCACCGCCT TAGAGTCAGT TGTGGCAGGT
    GGGAGAGTGT CTAGGGAAAA GGCGGAAGCC GACGCTGGCC GTGACTGTAC TTCCTACGAG
    CCTCTGCCCG CGTGGGCTCA GAAATTGCTC GGAAACTCCC TGTTGAAACT GGAAGGTGAT
    GACCGTCTGG ACATGAACTG CACGCTGCCC TTCAGCGACC AGGTGGTGCT GGTGGGCACC
    GAGGAAGGGC TGTATGCGCT GAATGTCTTG AAGAACTCCC TCACCCACGT CCCAGGAATC
    GGAGCAGTCT TCCAGATTTA CATCATCAAG GACCTGGAGA AGCTCCTCAT GATCGCAGGA
    GAGGAGCGGG CCCTGTGTCT CGTGGATGTG AAGAAGGTGA AGCAGTCCCT CGCGCAGTCT
    CACCTCCCCG CCCAGCCAGA CATCTCGCCC AACATCTTTG AAGCGGTCAA GGGCTGCCAC
    TTGTTTGCTG CTGGCAAGAT TGAGAGCGGG CTCTGCATCT GTGCAGCCAT GCCCAGCAAA
    GTTGTCATTC TCCGCTACAA TGAAAACCTC AGCAAGTACT GCATTCGCAA AGAGATCGAG
    ACCTCGGAGC CCTGCAGCTG TATCCACTTC ACCAACTACA GTATCCTCAT CGGAACCAAT
    AAATTCTACG AAATCGACAT GAAGCAGTAC ACGCTAGAGG AATTCCTGGA TAAGAACGAC
    CATTCCTTGG CGCCTGCCGT GTTTGCCTCC TCTTCCAACA GCTTCCCCGT GTCCATCATG
    CAGGTGAACG GCGCGGGGCA GCGGGAGGAG TTCCTGCTCT GTTTCCACGA ATTCGGGGTG
    TTCGTGGATT CTTATGGAAG ACGCAGCCGC ACAGATGATC TCAAGTGGAG TCGCTTACCT
    CTGGCCTTTG CCTACAGAGA ACCCTATCTG TTTGTGACTC ACTTCAACTC ACTCGAAGTA
    ATTGAGATCC AGGCCCGCTC CTCTCTGGGG ACCCCCGCCC GAGCATATTT GGAAATCCCC
    AACCCACGCT ACCTGGGCCC TGCAATCTCC TCAGGAGCCA TCTACCTGGC GTCCTCATAT
    CAGGATAAGT TAAGGGTCAT CTGCTGCAAA GGAAACCTGG TGAAGGAGAC CGGCACCGAC
    CACCACCGTG GCCCATCCAC CTCCCGCAGC CCCAACAAGC GAGGCCCGCC GACGTACAAC
    GAGCACATCA CCAAGCGCGT GGCCTCAAGC CCGGCGCCTC CCGAAGGCCC CAGCCACCCT
    CGAGAGCCAA GCACACCCCA CCGCTACCGC GAGGGGCGGA CCGAGCTGCG CAGGGACAAG
    TCTCCGGGCC GCCCCCTGGA GCGCGAGAAG TCCCCCGGCC GGATGCTGAG CACGCGGAGG
    GAGCGCTCCC CCGGGCGGCT GTTTGAGGAC AGCAGCAGGG GCCGGCTGCC CGTGGGGGCC
    GTGAGGACCC CGCTGTCCCA GGTCAACAAG GTCTGGGACC AGTCTTCAGT ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa224630
    Clone ID Related Accession (Same CDS sequence) XM_024977743.1 , XM_024977742.1
    Accession Version XM_024977742.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4914bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product citron Rho-interacting kinase isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGAAAAGA AACTCCTTAT CAAAAGCAAA GAGCTGCAGG ACTCCCAGGA CAAGTGTCAC 
    AAGATGGAGC AGGAAATGAC CCGGTTACAT CGGAGAGTGT CAGAGGTGGA GGCTGTGCTT
    AGTCAGAAGG AGGTGGAGCT GAAGGCCTCT GAGACTCAGA GATCCCTCCT GGAGCAGGAC
    CTTGCTACCT ACATCACAGA ATGCAGTAGC TTAAAGCGAA GTTTGGAGCA AGCACGGATG
    GAGGTGTCCC AAGAGGACGA CAAAGCACTG CAGCTCCTCC ATGATATCAG AGAGCAGAGC
    CGGAAGCTCC AAGAAATCAA AGAGCAGGAG TACCAGGCTC AAGTGGAAGA AATGAGGTTG
    ATGATGAATC AGTTGGAAGA GGACCTGGTT TCGGCGAGAA GGCGGAGTGA CCTCTATGAA
    TCGGAGCTCA GAGAGTCTCG GCTGGCTGCT GAAGAGTTTA AGCGGAAAGC AACAGAATGT
    CAGCATAAAC TGATGAAGGC TAAAGATCAA GGGAAGCCTG AAGTGGGAGA ATATGCCAAA
    CTGGAGAAGA TCAATGCTGA GCAGCAGCTC AAAATTCAGG AGCTCCAAGA GAAGCTAGAA
    AAGGCTGTGA AAGCTAGCAC AGAGGCCACC GAGCTGCTGC AGAACATCCG TCAGGCGAAG
    GAGCGGGCCG AGCGTGAGCT GGAGAAGCTG CACAACCGTG AGGACTCCTC CGAGGGCATC
    AAGAAGAAGC TGGTGGAGGC CGAGGAACGC CGCCACTCTC TGGAGAACAA GGTAAAGAGG
    CTAGAGACCA TGGAGCGTAG AGAAAACAGA CTGAAGGATG ACATCCAGAC AAAGTCCCAA
    CAGATCCAGC AGATGGCTGA TAAAATTCTG GAGCTGGAGG AGAAGCACAG AGAAGCCCAG
    GTCTCAGCGC AGCATCTGGA GGTGCACCTG AAACAGAAAG AGCAGCACTA TGAAGAAAAA
    ATTAAAGTGT TGGACAATCA GATAAAGAAA GACCTAGCAG ACAAGGAGAC ACTGGAGAAC
    CTGATGCAAA GACACGAAGA GGAGGCCCAT GAGAAAGGCA AAATTCTCAG CGAGCAGAAG
    GCGATGATCA ATGCTATGGA TTCCAAGATC AGATCCCTGG AACAAAGGAT AGTGGAATTG
    TCGGAAGCCA ATAAACTTGC GGCCAACAGC AGTCTCTTTA CCCAGAGGAA CATGAAGGCC
    CAGGAAGAGA TGATTTCAGA ACTCAGGCAA CAGAAGTTTT ACCTGGAGAC GCAAGCTGGG
    AAATTGGAGG CCCAGAACCG GAAGCTGGAG GAGCAGCTGG AAAAAATAAG CCACCAAGAC
    CACAGTGACA AGAACCGTCT GCTGGAGCTG GAGACCAGGC TAAGGGAGGT CAGTCTAGAA
    CATGAAGAGC AGAAACTGGA GCTCAAACGC CAGCTCACAG AGCTGCAGCT CTCCCTGCAG
    GAGCGTGAAT CCCAGCTGAC AGCCCTGCAG GCAGCACGGG CGGCCCTGGA GAGCCAGCTC
    CGCCAGGCCA AGACGGAGCT GGAAGAGACG ACAGCAGAAG CAGAAGAAGA GATCCAGGCG
    CTTACGGCAC ATAGAGATGA AATCCAGCGC AAATTCGATG CCCTTCGTAA CAGCTGTACT
    GTGATCACGG ACTTGGAGGA GCAGCTAAAC CAGCTGACGG AGGACAATGC CGAGCTCAAC
    AACCAAAACT TCTACTTGTC TAAACAACTC GATGAGGCCT CTGGTGCCAA CGACGAGATT
    GTGCAGCTGC GAAGTGAAGT GGACCATCTC CGCCGCGAGA TCACTGAGAG GGAGATGCAG
    CTCACTAGCC AGAAGCAAAC GATGGAGGCG CTGAAGACCA CGTGCACAAT GCTGGAGGAG
    CAGGTCATGG ACCTGGAGGC CCTGAACGAC GAGCTGCTGG AGAAGGAGCG GCAGTGGGAG
    GCCTGGAGGA GTGTCCTTGG TGACGAGAAG TCCCAGTTTG AGTGTCGGGT TCGAGAGTTG
    CAGAGGATGC TGGACACCGA AAAGCAGAGC AGGGCGAGGG CCGACCAGAG GATCACTGAG
    TCGCGCCAGG TGGTCGAGCT GGCAGTAAAG GAGCACAAGG CCGAGATCCT GGCCTTGCAG
    CAGGCTCTCA AGGAGCAGAA GCTGAAGGCC GAGAGCCTCT CCGACAAGCT CAATGATCTG
    GAGAAGAAGC ACGCCATGCT TGAAATGAAT GCCCGAAGTT TACAACAGAA GCTGGAGACA
    GAACGAGAGC TCAAACAAAG GCTTCTGGAA GAGCAAGCCA AGTTACAGCA GCAGATGGAC
    CTGCAGAAGA ATCATATTTT CCGTCTGACT CAAGGGCTGC AAGAAGCTCT GGATCGGGCT
    GATCTGCTGA AGACAGAAAG GAGTGACCTG GAATATCAGC TGGAAAACAT TCAGGTTCTC
    TATTCTCATG AGAAGGTGAA GATGGAAGGC ACTATTTCCC AGCAAACCAA ACTCATCGAT
    TTTCTGCAAG CCAAAATGGA CCAGCCTGCC AAAAAGAAAA AGGGTTTATT TAGTCGACGG
    AAAGAGGACC CTGCTTTGCC CACACAGGTT CCTCTGCAGT ACAATGAGCT GAAGGTGGCT
    CTGGAGAAGG AGAAAGCTCG CTGCGCGGAG CTCGAGGAAG CCCTGCAGAA GACCCGCATC
    GAGCTCCGCT CCGCCCGGGA GGAAGCTGCC CACCGGAAAG CTGCAGACCA CCCACACCCA
    TCTACGCCGG CTACCGCCAG GCAGCAGATC GCCATGTCCG CCATTGTGCG GTCACCCGAG
    CACCAGCCCA GCGCCCTGAG CCTGCTCGCC CCGCCGTCCA GCCGCAGGAA GGAGTCTTCC
    ACTCCAGAGG AATTCAGCCG GCGTCTTAAG GAGCGCATGC ACCACAACAT CCCTCACCGG
    TTCAACGTGG GACTGAACAT GCGAGCCACG AAGTGCGCCG TGTGTCTGGA TACCGTGCAC
    TTCGGGCGCC AGGCATCCAA GTGTCTCGAA TGTCAGGTCA TGTGCCACCC CAAGTGCTCC
    ACATGCTTGC CAGCCACCTG TGGCCTGCCA GCCGAATACG CCACACACTT CACCGAGGCC
    TTCTGCCGCG ACAAAATGAA CTCCCCGGGT CTGCAGTCCA AGGAGCCCAG CAGCAGCCTG
    CACCTGGAAG GGTGGATGAA GGTGCCCAGG AATAACAAGC GAGGACAGCA AGGCTGGGAC
    AGGAAGTACA TCGTTCTGGA GGGCTCCAAA GTCCTCATTT ATGACAACGA AGCCAGAGAA
    GCTGGACAGA GGCCGGTGGA AGAATTTGAG CTGTGCCTTC CCGACGGGGA CGTGTCTATT
    CATGGCGCCG TTGGTGCTTC TGAACTTGCA AACACAGCCA AAGCAGATGT CCCCTATGTC
    CTGAAGATGG AGTCTCACCC CCATACCACC TGCTGGCCTG GGAGGACCCT CTACTTGCTG
    GCTCCCAGCT TTCCCGACAA GCAGCGCTGG GTCACCGCCT TAGAGTCAGT TGTGGCAGGT
    GGGAGAGTGT CTAGGGAAAA GGCGGAAGCC GACGCTGGCC GTGACTGTAC TTCCTACGAG
    CCTCTGCCCG CGTGGGCTCA GAAATTGCTC GGAAACTCCC TGTTGAAACT GGAAGGTGAT
    GACCGTCTGG ACATGAACTG CACGCTGCCC TTCAGCGACC AGGTGGTGCT GGTGGGCACC
    GAGGAAGGGC TGTATGCGCT GAATGTCTTG AAGAACTCCC TCACCCACGT CCCAGGAATC
    GGAGCAGTCT TCCAGATTTA CATCATCAAG GACCTGGAGA AGCTCCTCAT GATCGCAGGA
    GAGGAGCGGG CCCTGTGTCT CGTGGATGTG AAGAAGGTGA AGCAGTCCCT CGCGCAGTCT
    CACCTCCCCG CCCAGCCAGA CATCTCGCCC AACATCTTTG AAGCGGTCAA GGGCTGCCAC
    TTGTTTGCTG CTGGCAAGAT TGAGAGCGGG CTCTGCATCT GTGCAGCCAT GCCCAGCAAA
    GTTGTCATTC TCCGCTACAA TGAAAACCTC AGCAAGTACT GCATTCGCAA AGAGATCGAG
    ACCTCGGAGC CCTGCAGCTG TATCCACTTC ACCAACTACA GTATCCTCAT CGGAACCAAT
    AAATTCTACG AAATCGACAT GAAGCAGTAC ACGCTAGAGG AATTCCTGGA TAAGAACGAC
    CATTCCTTGG CGCCTGCCGT GTTTGCCTCC TCTTCCAACA GCTTCCCCGT GTCCATCATG
    CAGGTGAACG GCGCGGGGCA GCGGGAGGAG TTCCTGCTCT GTTTCCACGA ATTCGGGGTG
    TTCGTGGATT CTTATGGAAG ACGCAGCCGC ACAGATGATC TCAAGTGGAG TCGCTTACCT
    CTGGCCTTTG CCTACAGAGA ACCCTATCTG TTTGTGACTC ACTTCAACTC ACTCGAAGTA
    ATTGAGATCC AGGCCCGCTC CTCTCTGGGG ACCCCCGCCC GAGCATATTT GGAAATCCCC
    AACCCACGCT ACCTGGGCCC TGCAATCTCC TCAGGAGCCA TCTACCTGGC GTCCTCATAT
    CAGGATAAGT TAAGGGTCAT CTGCTGCAAA GGAAACCTGG TGAAGGAGAC CGGCACCGAC
    CACCACCGTG GCCCATCCAC CTCCCGCAGC CCCAACAAGC GAGGCCCGCC GACGTACAAC
    GAGCACATCA CCAAGCGCGT GGCCTCAAGC CCGGCGCCTC CCGAAGGCCC CAGCCACCCT
    CGAGAGCCAA GCACACCCCA CCGCTACCGC GAGGGGCGGA CCGAGCTGCG CAGGGACAAG
    TCTCCGGGCC GCCCCCTGGA GCGCGAGAAG TCCCCCGGCC GGATGCTGAG CACGCGGAGG
    GAGCGCTCCC CCGGGCGGCT GTTTGAGGAC AGCAGCAGGG GCCGGCTGCC CGTGGGGGCC
    GTGAGGACCC CGCTGTCCCA GGTCAACAAG GTCTGGGACC AGTCTTCAGT ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024833510.1
    CDS542..5455
    Translation

    Target ORF information:

    RefSeq Version XM_024977742.1
    Organism Bos taurus(cattle)
    Definition Bos taurus citron rho-interacting serine/threonine kinase (CIT), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024977742.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGAAAAGA AACTCCTTAT CAAAAGCAAA GAGCTGCAGG ACTCCCAGGA CAAGTGTCAC 
    AAGATGGAGC AGGAAATGAC CCGGTTACAT CGGAGAGTGT CAGAGGTGGA GGCTGTGCTT
    AGTCAGAAGG AGGTGGAGCT GAAGGCCTCT GAGACTCAGA GATCCCTCCT GGAGCAGGAC
    CTTGCTACCT ACATCACAGA ATGCAGTAGC TTAAAGCGAA GTTTGGAGCA AGCACGGATG
    GAGGTGTCCC AAGAGGACGA CAAAGCACTG CAGCTCCTCC ATGATATCAG AGAGCAGAGC
    CGGAAGCTCC AAGAAATCAA AGAGCAGGAG TACCAGGCTC AAGTGGAAGA AATGAGGTTG
    ATGATGAATC AGTTGGAAGA GGACCTGGTT TCGGCGAGAA GGCGGAGTGA CCTCTATGAA
    TCGGAGCTCA GAGAGTCTCG GCTGGCTGCT GAAGAGTTTA AGCGGAAAGC AACAGAATGT
    CAGCATAAAC TGATGAAGGC TAAAGATCAA GGGAAGCCTG AAGTGGGAGA ATATGCCAAA
    CTGGAGAAGA TCAATGCTGA GCAGCAGCTC AAAATTCAGG AGCTCCAAGA GAAGCTAGAA
    AAGGCTGTGA AAGCTAGCAC AGAGGCCACC GAGCTGCTGC AGAACATCCG TCAGGCGAAG
    GAGCGGGCCG AGCGTGAGCT GGAGAAGCTG CACAACCGTG AGGACTCCTC CGAGGGCATC
    AAGAAGAAGC TGGTGGAGGC CGAGGAACGC CGCCACTCTC TGGAGAACAA GGTAAAGAGG
    CTAGAGACCA TGGAGCGTAG AGAAAACAGA CTGAAGGATG ACATCCAGAC AAAGTCCCAA
    CAGATCCAGC AGATGGCTGA TAAAATTCTG GAGCTGGAGG AGAAGCACAG AGAAGCCCAG
    GTCTCAGCGC AGCATCTGGA GGTGCACCTG AAACAGAAAG AGCAGCACTA TGAAGAAAAA
    ATTAAAGTGT TGGACAATCA GATAAAGAAA GACCTAGCAG ACAAGGAGAC ACTGGAGAAC
    CTGATGCAAA GACACGAAGA GGAGGCCCAT GAGAAAGGCA AAATTCTCAG CGAGCAGAAG
    GCGATGATCA ATGCTATGGA TTCCAAGATC AGATCCCTGG AACAAAGGAT AGTGGAATTG
    TCGGAAGCCA ATAAACTTGC GGCCAACAGC AGTCTCTTTA CCCAGAGGAA CATGAAGGCC
    CAGGAAGAGA TGATTTCAGA ACTCAGGCAA CAGAAGTTTT ACCTGGAGAC GCAAGCTGGG
    AAATTGGAGG CCCAGAACCG GAAGCTGGAG GAGCAGCTGG AAAAAATAAG CCACCAAGAC
    CACAGTGACA AGAACCGTCT GCTGGAGCTG GAGACCAGGC TAAGGGAGGT CAGTCTAGAA
    CATGAAGAGC AGAAACTGGA GCTCAAACGC CAGCTCACAG AGCTGCAGCT CTCCCTGCAG
    GAGCGTGAAT CCCAGCTGAC AGCCCTGCAG GCAGCACGGG CGGCCCTGGA GAGCCAGCTC
    CGCCAGGCCA AGACGGAGCT GGAAGAGACG ACAGCAGAAG CAGAAGAAGA GATCCAGGCG
    CTTACGGCAC ATAGAGATGA AATCCAGCGC AAATTCGATG CCCTTCGTAA CAGCTGTACT
    GTGATCACGG ACTTGGAGGA GCAGCTAAAC CAGCTGACGG AGGACAATGC CGAGCTCAAC
    AACCAAAACT TCTACTTGTC TAAACAACTC GATGAGGCCT CTGGTGCCAA CGACGAGATT
    GTGCAGCTGC GAAGTGAAGT GGACCATCTC CGCCGCGAGA TCACTGAGAG GGAGATGCAG
    CTCACTAGCC AGAAGCAAAC GATGGAGGCG CTGAAGACCA CGTGCACAAT GCTGGAGGAG
    CAGGTCATGG ACCTGGAGGC CCTGAACGAC GAGCTGCTGG AGAAGGAGCG GCAGTGGGAG
    GCCTGGAGGA GTGTCCTTGG TGACGAGAAG TCCCAGTTTG AGTGTCGGGT TCGAGAGTTG
    CAGAGGATGC TGGACACCGA AAAGCAGAGC AGGGCGAGGG CCGACCAGAG GATCACTGAG
    TCGCGCCAGG TGGTCGAGCT GGCAGTAAAG GAGCACAAGG CCGAGATCCT GGCCTTGCAG
    CAGGCTCTCA AGGAGCAGAA GCTGAAGGCC GAGAGCCTCT CCGACAAGCT CAATGATCTG
    GAGAAGAAGC ACGCCATGCT TGAAATGAAT GCCCGAAGTT TACAACAGAA GCTGGAGACA
    GAACGAGAGC TCAAACAAAG GCTTCTGGAA GAGCAAGCCA AGTTACAGCA GCAGATGGAC
    CTGCAGAAGA ATCATATTTT CCGTCTGACT CAAGGGCTGC AAGAAGCTCT GGATCGGGCT
    GATCTGCTGA AGACAGAAAG GAGTGACCTG GAATATCAGC TGGAAAACAT TCAGGTTCTC
    TATTCTCATG AGAAGGTGAA GATGGAAGGC ACTATTTCCC AGCAAACCAA ACTCATCGAT
    TTTCTGCAAG CCAAAATGGA CCAGCCTGCC AAAAAGAAAA AGGGTTTATT TAGTCGACGG
    AAAGAGGACC CTGCTTTGCC CACACAGGTT CCTCTGCAGT ACAATGAGCT GAAGGTGGCT
    CTGGAGAAGG AGAAAGCTCG CTGCGCGGAG CTCGAGGAAG CCCTGCAGAA GACCCGCATC
    GAGCTCCGCT CCGCCCGGGA GGAAGCTGCC CACCGGAAAG CTGCAGACCA CCCACACCCA
    TCTACGCCGG CTACCGCCAG GCAGCAGATC GCCATGTCCG CCATTGTGCG GTCACCCGAG
    CACCAGCCCA GCGCCCTGAG CCTGCTCGCC CCGCCGTCCA GCCGCAGGAA GGAGTCTTCC
    ACTCCAGAGG AATTCAGCCG GCGTCTTAAG GAGCGCATGC ACCACAACAT CCCTCACCGG
    TTCAACGTGG GACTGAACAT GCGAGCCACG AAGTGCGCCG TGTGTCTGGA TACCGTGCAC
    TTCGGGCGCC AGGCATCCAA GTGTCTCGAA TGTCAGGTCA TGTGCCACCC CAAGTGCTCC
    ACATGCTTGC CAGCCACCTG TGGCCTGCCA GCCGAATACG CCACACACTT CACCGAGGCC
    TTCTGCCGCG ACAAAATGAA CTCCCCGGGT CTGCAGTCCA AGGAGCCCAG CAGCAGCCTG
    CACCTGGAAG GGTGGATGAA GGTGCCCAGG AATAACAAGC GAGGACAGCA AGGCTGGGAC
    AGGAAGTACA TCGTTCTGGA GGGCTCCAAA GTCCTCATTT ATGACAACGA AGCCAGAGAA
    GCTGGACAGA GGCCGGTGGA AGAATTTGAG CTGTGCCTTC CCGACGGGGA CGTGTCTATT
    CATGGCGCCG TTGGTGCTTC TGAACTTGCA AACACAGCCA AAGCAGATGT CCCCTATGTC
    CTGAAGATGG AGTCTCACCC CCATACCACC TGCTGGCCTG GGAGGACCCT CTACTTGCTG
    GCTCCCAGCT TTCCCGACAA GCAGCGCTGG GTCACCGCCT TAGAGTCAGT TGTGGCAGGT
    GGGAGAGTGT CTAGGGAAAA GGCGGAAGCC GACGCTGGCC GTGACTGTAC TTCCTACGAG
    CCTCTGCCCG CGTGGGCTCA GAAATTGCTC GGAAACTCCC TGTTGAAACT GGAAGGTGAT
    GACCGTCTGG ACATGAACTG CACGCTGCCC TTCAGCGACC AGGTGGTGCT GGTGGGCACC
    GAGGAAGGGC TGTATGCGCT GAATGTCTTG AAGAACTCCC TCACCCACGT CCCAGGAATC
    GGAGCAGTCT TCCAGATTTA CATCATCAAG GACCTGGAGA AGCTCCTCAT GATCGCAGGA
    GAGGAGCGGG CCCTGTGTCT CGTGGATGTG AAGAAGGTGA AGCAGTCCCT CGCGCAGTCT
    CACCTCCCCG CCCAGCCAGA CATCTCGCCC AACATCTTTG AAGCGGTCAA GGGCTGCCAC
    TTGTTTGCTG CTGGCAAGAT TGAGAGCGGG CTCTGCATCT GTGCAGCCAT GCCCAGCAAA
    GTTGTCATTC TCCGCTACAA TGAAAACCTC AGCAAGTACT GCATTCGCAA AGAGATCGAG
    ACCTCGGAGC CCTGCAGCTG TATCCACTTC ACCAACTACA GTATCCTCAT CGGAACCAAT
    AAATTCTACG AAATCGACAT GAAGCAGTAC ACGCTAGAGG AATTCCTGGA TAAGAACGAC
    CATTCCTTGG CGCCTGCCGT GTTTGCCTCC TCTTCCAACA GCTTCCCCGT GTCCATCATG
    CAGGTGAACG GCGCGGGGCA GCGGGAGGAG TTCCTGCTCT GTTTCCACGA ATTCGGGGTG
    TTCGTGGATT CTTATGGAAG ACGCAGCCGC ACAGATGATC TCAAGTGGAG TCGCTTACCT
    CTGGCCTTTG CCTACAGAGA ACCCTATCTG TTTGTGACTC ACTTCAACTC ACTCGAAGTA
    ATTGAGATCC AGGCCCGCTC CTCTCTGGGG ACCCCCGCCC GAGCATATTT GGAAATCCCC
    AACCCACGCT ACCTGGGCCC TGCAATCTCC TCAGGAGCCA TCTACCTGGC GTCCTCATAT
    CAGGATAAGT TAAGGGTCAT CTGCTGCAAA GGAAACCTGG TGAAGGAGAC CGGCACCGAC
    CACCACCGTG GCCCATCCAC CTCCCGCAGC CCCAACAAGC GAGGCCCGCC GACGTACAAC
    GAGCACATCA CCAAGCGCGT GGCCTCAAGC CCGGCGCCTC CCGAAGGCCC CAGCCACCCT
    CGAGAGCCAA GCACACCCCA CCGCTACCGC GAGGGGCGGA CCGAGCTGCG CAGGGACAAG
    TCTCCGGGCC GCCCCCTGGA GCGCGAGAAG TCCCCCGGCC GGATGCTGAG CACGCGGAGG
    GAGCGCTCCC CCGGGCGGCT GTTTGAGGAC AGCAGCAGGG GCCGGCTGCC CGTGGGGGCC
    GTGAGGACCC CGCTGTCCCA GGTCAACAAG GTCTGGGACC AGTCTTCAGT ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa224631
    Clone ID Related Accession (Same CDS sequence) XM_024977744.1
    Accession Version XM_024977744.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4854bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product citron Rho-interacting kinase isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGATGGAGC AGGAAATGAC CCGGTTACAT CGGAGAGTGT CAGAGGTGGA GGCTGTGCTT 
    AGTCAGAAGG AGGTGGAGCT GAAGGCCTCT GAGACTCAGA GATCCCTCCT GGAGCAGGAC
    CTTGCTACCT ACATCACAGA ATGCAGTAGC TTAAAGCGAA GTTTGGAGCA AGCACGGATG
    GAGGTGTCCC AAGAGGACGA CAAAGCACTG CAGCTCCTCC ATGATATCAG AGAGCAGAGC
    CGGAAGCTCC AAGAAATCAA AGAGCAGGAG TACCAGGCTC AAGTGGAAGA AATGAGGTTG
    ATGATGAATC AGTTGGAAGA GGACCTGGTT TCGGCGAGAA GGCGGAGTGA CCTCTATGAA
    TCGGAGCTCA GAGAGTCTCG GCTGGCTGCT GAAGAGTTTA AGCGGAAAGC AACAGAATGT
    CAGCATAAAC TGATGAAGGC TAAAGATCAA GGGAAGCCTG AAGTGGGAGA ATATGCCAAA
    CTGGAGAAGA TCAATGCTGA GCAGCAGCTC AAAATTCAGG AGCTCCAAGA GAAGCTAGAA
    AAGGCTGTGA AAGCTAGCAC AGAGGCCACC GAGCTGCTGC AGAACATCCG TCAGGCGAAG
    GAGCGGGCCG AGCGTGAGCT GGAGAAGCTG CACAACCGTG AGGACTCCTC CGAGGGCATC
    AAGAAGAAGC TGGTGGAGGC CGAGGAACGC CGCCACTCTC TGGAGAACAA GGTAAAGAGG
    CTAGAGACCA TGGAGCGTAG AGAAAACAGA CTGAAGGATG ACATCCAGAC AAAGTCCCAA
    CAGATCCAGC AGATGGCTGA TAAAATTCTG GAGCTGGAGG AGAAGCACAG AGAAGCCCAG
    GTCTCAGCGC AGCATCTGGA GGTGCACCTG AAACAGAAAG AGCAGCACTA TGAAGAAAAA
    ATTAAAGTGT TGGACAATCA GATAAAGAAA GACCTAGCAG ACAAGGAGAC ACTGGAGAAC
    CTGATGCAAA GACACGAAGA GGAGGCCCAT GAGAAAGGCA AAATTCTCAG CGAGCAGAAG
    GCGATGATCA ATGCTATGGA TTCCAAGATC AGATCCCTGG AACAAAGGAT AGTGGAATTG
    TCGGAAGCCA ATAAACTTGC GGCCAACAGC AGTCTCTTTA CCCAGAGGAA CATGAAGGCC
    CAGGAAGAGA TGATTTCAGA ACTCAGGCAA CAGAAGTTTT ACCTGGAGAC GCAAGCTGGG
    AAATTGGAGG CCCAGAACCG GAAGCTGGAG GAGCAGCTGG AAAAAATAAG CCACCAAGAC
    CACAGTGACA AGAACCGTCT GCTGGAGCTG GAGACCAGGC TAAGGGAGGT CAGTCTAGAA
    CATGAAGAGC AGAAACTGGA GCTCAAACGC CAGCTCACAG AGCTGCAGCT CTCCCTGCAG
    GAGCGTGAAT CCCAGCTGAC AGCCCTGCAG GCAGCACGGG CGGCCCTGGA GAGCCAGCTC
    CGCCAGGCCA AGACGGAGCT GGAAGAGACG ACAGCAGAAG CAGAAGAAGA GATCCAGGCG
    CTTACGGCAC ATAGAGATGA AATCCAGCGC AAATTCGATG CCCTTCGTAA CAGCTGTACT
    GTGATCACGG ACTTGGAGGA GCAGCTAAAC CAGCTGACGG AGGACAATGC CGAGCTCAAC
    AACCAAAACT TCTACTTGTC TAAACAACTC GATGAGGCCT CTGGTGCCAA CGACGAGATT
    GTGCAGCTGC GAAGTGAAGT GGACCATCTC CGCCGCGAGA TCACTGAGAG GGAGATGCAG
    CTCACTAGCC AGAAGCAAAC GATGGAGGCG CTGAAGACCA CGTGCACAAT GCTGGAGGAG
    CAGGTCATGG ACCTGGAGGC CCTGAACGAC GAGCTGCTGG AGAAGGAGCG GCAGTGGGAG
    GCCTGGAGGA GTGTCCTTGG TGACGAGAAG TCCCAGTTTG AGTGTCGGGT TCGAGAGTTG
    CAGAGGATGC TGGACACCGA AAAGCAGAGC AGGGCGAGGG CCGACCAGAG GATCACTGAG
    TCGCGCCAGG TGGTCGAGCT GGCAGTAAAG GAGCACAAGG CCGAGATCCT GGCCTTGCAG
    CAGGCTCTCA AGGAGCAGAA GCTGAAGGCC GAGAGCCTCT CCGACAAGCT CAATGATCTG
    GAGAAGAAGC ACGCCATGCT TGAAATGAAT GCCCGAAGTT TACAACAGAA GCTGGAGACA
    GAACGAGAGC TCAAACAAAG GCTTCTGGAA GAGCAAGCCA AGTTACAGCA GCAGATGGAC
    CTGCAGAAGA ATCATATTTT CCGTCTGACT CAAGGGCTGC AAGAAGCTCT GGATCGGGCT
    GATCTGCTGA AGACAGAAAG GAGTGACCTG GAATATCAGC TGGAAAACAT TCAGGTTCTC
    TATTCTCATG AGAAGGTGAA GATGGAAGGC ACTATTTCCC AGCAAACCAA ACTCATCGAT
    TTTCTGCAAG CCAAAATGGA CCAGCCTGCC AAAAAGAAAA AGGGTTTATT TAGTCGACGG
    AAAGAGGACC CTGCTTTGCC CACACAGGTT CCTCTGCAGT ACAATGAGCT GAAGGTGGCT
    CTGGAGAAGG AGAAAGCTCG CTGCGCGGAG CTCGAGGAAG CCCTGCAGAA GACCCGCATC
    GAGCTCCGCT CCGCCCGGGA GGAAGCTGCC CACCGGAAAG CTGCAGACCA CCCACACCCA
    TCTACGCCGG CTACCGCCAG GCAGCAGATC GCCATGTCCG CCATTGTGCG GTCACCCGAG
    CACCAGCCCA GCGCCCTGAG CCTGCTCGCC CCGCCGTCCA GCCGCAGGAA GGAGTCTTCC
    ACTCCAGAGG AATTCAGCCG GCGTCTTAAG GAGCGCATGC ACCACAACAT CCCTCACCGG
    TTCAACGTGG GACTGAACAT GCGAGCCACG AAGTGCGCCG TGTGTCTGGA TACCGTGCAC
    TTCGGGCGCC AGGCATCCAA GTGTCTCGAA TGTCAGGTCA TGTGCCACCC CAAGTGCTCC
    ACATGCTTGC CAGCCACCTG TGGCCTGCCA GCCGAATACG CCACACACTT CACCGAGGCC
    TTCTGCCGCG ACAAAATGAA CTCCCCGGGT CTGCAGTCCA AGGAGCCCAG CAGCAGCCTG
    CACCTGGAAG GGTGGATGAA GGTGCCCAGG AATAACAAGC GAGGACAGCA AGGCTGGGAC
    AGGAAGTACA TCGTTCTGGA GGGCTCCAAA GTCCTCATTT ATGACAACGA AGCCAGAGAA
    GCTGGACAGA GGCCGGTGGA AGAATTTGAG CTGTGCCTTC CCGACGGGGA CGTGTCTATT
    CATGGCGCCG TTGGTGCTTC TGAACTTGCA AACACAGCCA AAGCAGATGT CCCCTATGTC
    CTGAAGATGG AGTCTCACCC CCATACCACC TGCTGGCCTG GGAGGACCCT CTACTTGCTG
    GCTCCCAGCT TTCCCGACAA GCAGCGCTGG GTCACCGCCT TAGAGTCAGT TGTGGCAGGT
    GGGAGAGTGT CTAGGGAAAA GGCGGAAGCC GACGCTGGCC GTGACTGTAC TTCCTACGAG
    CCTCTGCCCG CGTGGGCTCA GAAATTGCTC GGAAACTCCC TGTTGAAACT GGAAGGTGAT
    GACCGTCTGG ACATGAACTG CACGCTGCCC TTCAGCGACC AGGTGGTGCT GGTGGGCACC
    GAGGAAGGGC TGTATGCGCT GAATGTCTTG AAGAACTCCC TCACCCACGT CCCAGGAATC
    GGAGCAGTCT TCCAGATTTA CATCATCAAG GACCTGGAGA AGCTCCTCAT GATCGCAGGA
    GAGGAGCGGG CCCTGTGTCT CGTGGATGTG AAGAAGGTGA AGCAGTCCCT CGCGCAGTCT
    CACCTCCCCG CCCAGCCAGA CATCTCGCCC AACATCTTTG AAGCGGTCAA GGGCTGCCAC
    TTGTTTGCTG CTGGCAAGAT TGAGAGCGGG CTCTGCATCT GTGCAGCCAT GCCCAGCAAA
    GTTGTCATTC TCCGCTACAA TGAAAACCTC AGCAAGTACT GCATTCGCAA AGAGATCGAG
    ACCTCGGAGC CCTGCAGCTG TATCCACTTC ACCAACTACA GTATCCTCAT CGGAACCAAT
    AAATTCTACG AAATCGACAT GAAGCAGTAC ACGCTAGAGG AATTCCTGGA TAAGAACGAC
    CATTCCTTGG CGCCTGCCGT GTTTGCCTCC TCTTCCAACA GCTTCCCCGT GTCCATCATG
    CAGGTGAACG GCGCGGGGCA GCGGGAGGAG TTCCTGCTCT GTTTCCACGA ATTCGGGGTG
    TTCGTGGATT CTTATGGAAG ACGCAGCCGC ACAGATGATC TCAAGTGGAG TCGCTTACCT
    CTGGCCTTTG CCTACAGAGA ACCCTATCTG TTTGTGACTC ACTTCAACTC ACTCGAAGTA
    ATTGAGATCC AGGCCCGCTC CTCTCTGGGG ACCCCCGCCC GAGCATATTT GGAAATCCCC
    AACCCACGCT ACCTGGGCCC TGCAATCTCC TCAGGAGCCA TCTACCTGGC GTCCTCATAT
    CAGGATAAGT TAAGGGTCAT CTGCTGCAAA GGAAACCTGG TGAAGGAGAC CGGCACCGAC
    CACCACCGTG GCCCATCCAC CTCCCGCAGC CCCAACAAGC GAGGCCCGCC GACGTACAAC
    GAGCACATCA CCAAGCGCGT GGCCTCAAGC CCGGCGCCTC CCGAAGGCCC CAGCCACCCT
    CGAGAGCCAA GCACACCCCA CCGCTACCGC GAGGGGCGGA CCGAGCTGCG CAGGGACAAG
    TCTCCGGGCC GCCCCCTGGA GCGCGAGAAG TCCCCCGGCC GGATGCTGAG CACGCGGAGG
    GAGCGCTCCC CCGGGCGGCT GTTTGAGGAC AGCAGCAGGG GCCGGCTGCC CGTGGGGGCC
    GTGAGGACCC CGCTGTCCCA GGTCAACAAG GTCTGGGACC AGTCTTCAGT ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024833512.1
    CDS330..5183
    Translation

    Target ORF information:

    RefSeq Version XM_024977744.1
    Organism Bos taurus(cattle)
    Definition Bos taurus citron rho-interacting serine/threonine kinase (CIT), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024977744.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGATGGAGC AGGAAATGAC CCGGTTACAT CGGAGAGTGT CAGAGGTGGA GGCTGTGCTT 
    AGTCAGAAGG AGGTGGAGCT GAAGGCCTCT GAGACTCAGA GATCCCTCCT GGAGCAGGAC
    CTTGCTACCT ACATCACAGA ATGCAGTAGC TTAAAGCGAA GTTTGGAGCA AGCACGGATG
    GAGGTGTCCC AAGAGGACGA CAAAGCACTG CAGCTCCTCC ATGATATCAG AGAGCAGAGC
    CGGAAGCTCC AAGAAATCAA AGAGCAGGAG TACCAGGCTC AAGTGGAAGA AATGAGGTTG
    ATGATGAATC AGTTGGAAGA GGACCTGGTT TCGGCGAGAA GGCGGAGTGA CCTCTATGAA
    TCGGAGCTCA GAGAGTCTCG GCTGGCTGCT GAAGAGTTTA AGCGGAAAGC AACAGAATGT
    CAGCATAAAC TGATGAAGGC TAAAGATCAA GGGAAGCCTG AAGTGGGAGA ATATGCCAAA
    CTGGAGAAGA TCAATGCTGA GCAGCAGCTC AAAATTCAGG AGCTCCAAGA GAAGCTAGAA
    AAGGCTGTGA AAGCTAGCAC AGAGGCCACC GAGCTGCTGC AGAACATCCG TCAGGCGAAG
    GAGCGGGCCG AGCGTGAGCT GGAGAAGCTG CACAACCGTG AGGACTCCTC CGAGGGCATC
    AAGAAGAAGC TGGTGGAGGC CGAGGAACGC CGCCACTCTC TGGAGAACAA GGTAAAGAGG
    CTAGAGACCA TGGAGCGTAG AGAAAACAGA CTGAAGGATG ACATCCAGAC AAAGTCCCAA
    CAGATCCAGC AGATGGCTGA TAAAATTCTG GAGCTGGAGG AGAAGCACAG AGAAGCCCAG
    GTCTCAGCGC AGCATCTGGA GGTGCACCTG AAACAGAAAG AGCAGCACTA TGAAGAAAAA
    ATTAAAGTGT TGGACAATCA GATAAAGAAA GACCTAGCAG ACAAGGAGAC ACTGGAGAAC
    CTGATGCAAA GACACGAAGA GGAGGCCCAT GAGAAAGGCA AAATTCTCAG CGAGCAGAAG
    GCGATGATCA ATGCTATGGA TTCCAAGATC AGATCCCTGG AACAAAGGAT AGTGGAATTG
    TCGGAAGCCA ATAAACTTGC GGCCAACAGC AGTCTCTTTA CCCAGAGGAA CATGAAGGCC
    CAGGAAGAGA TGATTTCAGA ACTCAGGCAA CAGAAGTTTT ACCTGGAGAC GCAAGCTGGG
    AAATTGGAGG CCCAGAACCG GAAGCTGGAG GAGCAGCTGG AAAAAATAAG CCACCAAGAC
    CACAGTGACA AGAACCGTCT GCTGGAGCTG GAGACCAGGC TAAGGGAGGT CAGTCTAGAA
    CATGAAGAGC AGAAACTGGA GCTCAAACGC CAGCTCACAG AGCTGCAGCT CTCCCTGCAG
    GAGCGTGAAT CCCAGCTGAC AGCCCTGCAG GCAGCACGGG CGGCCCTGGA GAGCCAGCTC
    CGCCAGGCCA AGACGGAGCT GGAAGAGACG ACAGCAGAAG CAGAAGAAGA GATCCAGGCG
    CTTACGGCAC ATAGAGATGA AATCCAGCGC AAATTCGATG CCCTTCGTAA CAGCTGTACT
    GTGATCACGG ACTTGGAGGA GCAGCTAAAC CAGCTGACGG AGGACAATGC CGAGCTCAAC
    AACCAAAACT TCTACTTGTC TAAACAACTC GATGAGGCCT CTGGTGCCAA CGACGAGATT
    GTGCAGCTGC GAAGTGAAGT GGACCATCTC CGCCGCGAGA TCACTGAGAG GGAGATGCAG
    CTCACTAGCC AGAAGCAAAC GATGGAGGCG CTGAAGACCA CGTGCACAAT GCTGGAGGAG
    CAGGTCATGG ACCTGGAGGC CCTGAACGAC GAGCTGCTGG AGAAGGAGCG GCAGTGGGAG
    GCCTGGAGGA GTGTCCTTGG TGACGAGAAG TCCCAGTTTG AGTGTCGGGT TCGAGAGTTG
    CAGAGGATGC TGGACACCGA AAAGCAGAGC AGGGCGAGGG CCGACCAGAG GATCACTGAG
    TCGCGCCAGG TGGTCGAGCT GGCAGTAAAG GAGCACAAGG CCGAGATCCT GGCCTTGCAG
    CAGGCTCTCA AGGAGCAGAA GCTGAAGGCC GAGAGCCTCT CCGACAAGCT CAATGATCTG
    GAGAAGAAGC ACGCCATGCT TGAAATGAAT GCCCGAAGTT TACAACAGAA GCTGGAGACA
    GAACGAGAGC TCAAACAAAG GCTTCTGGAA GAGCAAGCCA AGTTACAGCA GCAGATGGAC
    CTGCAGAAGA ATCATATTTT CCGTCTGACT CAAGGGCTGC AAGAAGCTCT GGATCGGGCT
    GATCTGCTGA AGACAGAAAG GAGTGACCTG GAATATCAGC TGGAAAACAT TCAGGTTCTC
    TATTCTCATG AGAAGGTGAA GATGGAAGGC ACTATTTCCC AGCAAACCAA ACTCATCGAT
    TTTCTGCAAG CCAAAATGGA CCAGCCTGCC AAAAAGAAAA AGGGTTTATT TAGTCGACGG
    AAAGAGGACC CTGCTTTGCC CACACAGGTT CCTCTGCAGT ACAATGAGCT GAAGGTGGCT
    CTGGAGAAGG AGAAAGCTCG CTGCGCGGAG CTCGAGGAAG CCCTGCAGAA GACCCGCATC
    GAGCTCCGCT CCGCCCGGGA GGAAGCTGCC CACCGGAAAG CTGCAGACCA CCCACACCCA
    TCTACGCCGG CTACCGCCAG GCAGCAGATC GCCATGTCCG CCATTGTGCG GTCACCCGAG
    CACCAGCCCA GCGCCCTGAG CCTGCTCGCC CCGCCGTCCA GCCGCAGGAA GGAGTCTTCC
    ACTCCAGAGG AATTCAGCCG GCGTCTTAAG GAGCGCATGC ACCACAACAT CCCTCACCGG
    TTCAACGTGG GACTGAACAT GCGAGCCACG AAGTGCGCCG TGTGTCTGGA TACCGTGCAC
    TTCGGGCGCC AGGCATCCAA GTGTCTCGAA TGTCAGGTCA TGTGCCACCC CAAGTGCTCC
    ACATGCTTGC CAGCCACCTG TGGCCTGCCA GCCGAATACG CCACACACTT CACCGAGGCC
    TTCTGCCGCG ACAAAATGAA CTCCCCGGGT CTGCAGTCCA AGGAGCCCAG CAGCAGCCTG
    CACCTGGAAG GGTGGATGAA GGTGCCCAGG AATAACAAGC GAGGACAGCA AGGCTGGGAC
    AGGAAGTACA TCGTTCTGGA GGGCTCCAAA GTCCTCATTT ATGACAACGA AGCCAGAGAA
    GCTGGACAGA GGCCGGTGGA AGAATTTGAG CTGTGCCTTC CCGACGGGGA CGTGTCTATT
    CATGGCGCCG TTGGTGCTTC TGAACTTGCA AACACAGCCA AAGCAGATGT CCCCTATGTC
    CTGAAGATGG AGTCTCACCC CCATACCACC TGCTGGCCTG GGAGGACCCT CTACTTGCTG
    GCTCCCAGCT TTCCCGACAA GCAGCGCTGG GTCACCGCCT TAGAGTCAGT TGTGGCAGGT
    GGGAGAGTGT CTAGGGAAAA GGCGGAAGCC GACGCTGGCC GTGACTGTAC TTCCTACGAG
    CCTCTGCCCG CGTGGGCTCA GAAATTGCTC GGAAACTCCC TGTTGAAACT GGAAGGTGAT
    GACCGTCTGG ACATGAACTG CACGCTGCCC TTCAGCGACC AGGTGGTGCT GGTGGGCACC
    GAGGAAGGGC TGTATGCGCT GAATGTCTTG AAGAACTCCC TCACCCACGT CCCAGGAATC
    GGAGCAGTCT TCCAGATTTA CATCATCAAG GACCTGGAGA AGCTCCTCAT GATCGCAGGA
    GAGGAGCGGG CCCTGTGTCT CGTGGATGTG AAGAAGGTGA AGCAGTCCCT CGCGCAGTCT
    CACCTCCCCG CCCAGCCAGA CATCTCGCCC AACATCTTTG AAGCGGTCAA GGGCTGCCAC
    TTGTTTGCTG CTGGCAAGAT TGAGAGCGGG CTCTGCATCT GTGCAGCCAT GCCCAGCAAA
    GTTGTCATTC TCCGCTACAA TGAAAACCTC AGCAAGTACT GCATTCGCAA AGAGATCGAG
    ACCTCGGAGC CCTGCAGCTG TATCCACTTC ACCAACTACA GTATCCTCAT CGGAACCAAT
    AAATTCTACG AAATCGACAT GAAGCAGTAC ACGCTAGAGG AATTCCTGGA TAAGAACGAC
    CATTCCTTGG CGCCTGCCGT GTTTGCCTCC TCTTCCAACA GCTTCCCCGT GTCCATCATG
    CAGGTGAACG GCGCGGGGCA GCGGGAGGAG TTCCTGCTCT GTTTCCACGA ATTCGGGGTG
    TTCGTGGATT CTTATGGAAG ACGCAGCCGC ACAGATGATC TCAAGTGGAG TCGCTTACCT
    CTGGCCTTTG CCTACAGAGA ACCCTATCTG TTTGTGACTC ACTTCAACTC ACTCGAAGTA
    ATTGAGATCC AGGCCCGCTC CTCTCTGGGG ACCCCCGCCC GAGCATATTT GGAAATCCCC
    AACCCACGCT ACCTGGGCCC TGCAATCTCC TCAGGAGCCA TCTACCTGGC GTCCTCATAT
    CAGGATAAGT TAAGGGTCAT CTGCTGCAAA GGAAACCTGG TGAAGGAGAC CGGCACCGAC
    CACCACCGTG GCCCATCCAC CTCCCGCAGC CCCAACAAGC GAGGCCCGCC GACGTACAAC
    GAGCACATCA CCAAGCGCGT GGCCTCAAGC CCGGCGCCTC CCGAAGGCCC CAGCCACCCT
    CGAGAGCCAA GCACACCCCA CCGCTACCGC GAGGGGCGGA CCGAGCTGCG CAGGGACAAG
    TCTCCGGGCC GCCCCCTGGA GCGCGAGAAG TCCCCCGGCC GGATGCTGAG CACGCGGAGG
    GAGCGCTCCC CCGGGCGGCT GTTTGAGGAC AGCAGCAGGG GCCGGCTGCC CGTGGGGGCC
    GTGAGGACCC CGCTGTCCCA GGTCAACAAG GTCTGGGACC AGTCTTCAGT ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa93236
    Clone ID Related Accession (Same CDS sequence) XM_015475534.1 , XM_015467110.1
    Accession Version XM_015475534.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5979bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product citron Rho-interacting kinase
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104457.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    ATGTTGAAGT TCAAGTATGG AGCACGGAAT CTACTGGACG CTGGTACTGT TGAATCCATT 
    GCCAGCCGGG CCTCCAGGCT GAATCTTTTC TTCCAGGGGA AACCACCCTC CATGACTCAA
    CAGCAGATGT CTCTTCTTTC CCGAGAAGCG ATATTAGATG CCCTCTTTGT TCTCTTTAAA
    GAATGCAGTC AGCCAGCTCT GATGAAGATT AAGCACGTGA GCAACTTTGT CCAAAAGTGT
    AAGTGTGGGG AGAACTTTTT CTTGAAAATT GTCCTGCTTA GTGAGAATGT CATGGAATAT
    CAGCCTGGAG GGGATTTGCT GTCACTTTTG AATAGATATG AGGACCAATT AGATGAAAAT
    ATGATTCAGT TTTACCTAGC TGAACTGATT TTGGCTGTCC ACAGCGTTCA TCAGATGGGC
    TATGTACATC GAGACATCAA GCCCGAGAAC ATCCTCATTG ACCGAACGGG ACACATCAAG
    CTGGTGGATT TTGGATCAGC TGCTAAAATG AACTCAAATA AGATGGTAAA TGCCAAACTC
    CCGGTTGGTA CTCCAGATTA CATGGCCCCT GAAGTGTTGA CTGTGATGAA TGGGGATGGA
    AAAGGTGCCT ATAGCCTAGA CTGTGATTGG TGGTCAGTGG GAGTTATTGC TTATGAGATG
    GTTTATGGAA GATGCCCATT CACTGAAGGA ACCTCAGCCA AAACCTTCAA TAACATCATG
    AATTTCCAGC GGTTCTTGAA GTTTCCAGAT GACCCCAAAG TTAGCAGTGA ATTGCTTGAT
    CTAATCCAGA GTTTGTTGTG TGGCCAGAAA GAGAGACTGA AGTTTGAGGG CCTTTGCTGT
    CATCCTTTCT TCTCCAAAAT TGACTGGATT AACATTCGTA ACTCTCCTCC CCCCTTCGTT
    CCCACCCTCA AGTCTGATGA TGACACCTCC AATTTTGATG AACCAGAGAA GAATTCGTGG
    GTTTCATCCT CTCCGTGCCA GCTGAACCCC TCAGGTTTCT CGGGCGAAGA ACTGCCGTTT
    GTGGGGTTTT CGTATAGCAA GGCACTGGGA ATTCTTGGTA GATCTGAGCC TGTTGTATCA
    AGTCTGGACT CCCCTGCCAA GACTAGCTCC ATGGAAAAGA AACTCCTTAT CAAAAGCAAA
    GAGCTGCAGG ACTCCCAGGA CAAGTGTCAC AAGATGGAGC AGGAAATGAC CCGGTTACAT
    CGGAGAGTGT CAGAGGTGGA GGCTGTGCTT AGTCAGAAGG AGGTGGAGCT GAAGGCCTCT
    GAGACTCAGA GATCCCTCCT GGAGCAGGAC CTTGCTACCT ACATCACAGA ATGCAGTAGC
    TTAAAGCGAA GTTTGGAGCA AGCACGGATG GAGGTGTCCC AAGAGGACGA CAAAGCACTG
    CAGCTCCTCC ATGATATCAG AGAGCAGAGC CGGAAGCTCC AAGAAATCAA AGAGCAGGAG
    TACCAGGCTC AAGTGGAAGA AATGAGGTTG ATGATGAATC AGTTGGAAGA GGACCTGGTT
    TCGGCGAGAA GGCGGAGTGA CCTCTATGAA TCGGAGCTCA GAGAGTCTCG GCTGGCTGCT
    GAAGAGTTTA AGCGGAAAGC AACAGAATGT CAGCATAAAC TGATGAAGGC TAAAGATCAA
    GGGAAGCCTG AAGTGGGAGA ATATGCCAAA CTGGAGAAGA TCAATGCTGA GCAGCAGCTC
    AAAATTCAGG AGCTCCAAGA GAAGCTAGAA AAGGCTGTGA AAGCTAGCAC AGAGGCCACC
    GAGCTGCTGC AGAACATCCG TCAGGCGAAG GAGCGGGCCG AGCGTGAGCT GGAGAAGCTG
    CACAACCGTG AGGACTCCTC CGAGGGCATC AAGAAGAAGC TGGTGGAGGC CGAGGAACGC
    CGCCACTCTC TGGAGAACAA GGTAAAGAGG CTAGAGACCA TGGAGCGTAG AGAAAACAGA
    CTGAAGGATG ACATCCAGAC AAAGTCCCAA CAGATCCAGC AGATGGCTGA TAAAATTCTG
    GAGCTGGAGG AGAAGCACAG AGAAGCCCAG GTCTCAGCGC AGCATCTGGA GGTGCACCTG
    AAACAGAAAG AGCAGCACTA TGAAGAAAAA ATTAAAGTGT TGGACAATCA GATAAAGAAA
    GACCTAGCAG ACAAGGAGAC ACTGGAGAAC CTGATGCAAA GACACGAAGA GGAGGCCCAT
    GAGAAAGGCA AAATTCTCAG CGAGCAGAAG GCGATGATCA ATGCTATGGA TTCCAAGATC
    AGATCCCTGG AACAAAGGAT AGTGGAATTG TCGGAAGCCA ATAAACTTGC GGCCAACAGC
    AGTCTCTTTA CCCAGAGGAA CATGAAGGCC CAGGAAGAGA TGATTTCAGA ACTCAGGCAA
    CAGAAGTTTT ACCTGGAGAC GCAAGCTGGG AAATTGGAGG CCCAGAACCG GAAGCTGGAG
    GAGCAGCTGG AAAAAATAAG CCACCAAGAC CACAGTGACA AGAACCGTCT GCTGGAGCTG
    GAGACCAGGC TAAGGGAGGT CAGTCTAGAA CATGAAGAGC AGAAACTGGA GCTCAAACGC
    CAGCTCACAG AGCTGCAGCT CTCCCTGCAG GAGCGTGAAT CCCAGCTGAC AGCCCTGCAG
    GCAGCACGGG CGGCCCTGGA GAGCCAGCTC CGCCAGGCCA AGACGGAGCT GGAAGAGACG
    ACAGCAGAAG CAGAAGAAGA GATCCAGGCG CTTACGGCAC ATAGAGATGA AATCCAGCGC
    AAATTCGATG CCCTTCGTAA CAGCTGTACT GTGATCACGG ACTTGGAGGA GCAGCTAAAC
    CAGCTGACGG AGGACAATGC CGAGCTCAAC AACCAAAACT TCTACTTGTC TAAACAACTC
    GATGAGGCCT CTGGTGCCAA CGACGAGATT GTGCAGCTGC GAAGTGAAGT GGACCATCTC
    CGCCGCGAGA TCACTGAGAG GGAGATGCAG CTCACTAGCC AGAAGCAAAC GATGGAGGCG
    CTGAAGACCA CGTGCACAAT GCTGGAGGAG CAGGTCATGG ACCTGGAGGC CCTGAACGAC
    GAGCTGCTGG AGAAGGAGCG GCAGTGGGAG GCCTGGAGGA GTGTCCTTGG TGACGAGAAG
    TCCCAGTTTG AGTGTCGGGT TCGAGAGTTG CAGAGGATGC TGGACACCGA AAAGCAGAGC
    AGGGCGAGGG CCGACCAGAG GATCACTGAG TCGCGCCAGG TGGTCGAGCT GGCAGTAAAG
    GAGCACAAGG CCGAGATCCT GGCCTTGCAG CAGGCTCTCA AGGAGCAGAA GCTGAAGGCC
    GAGAGCCTCT CCGACAAGCT CAATGATCTG GAGAAGAAGC ACGCCATGCT TGAAATGAAT
    GCCCGAAGTT TACAACAGAA GCTGGAGACA GAACGAGAGC TCAAACAAAG GCTTCTGGAA
    GAGCAAGCCA AGTTACAGCA GCAGATGGAC CTGCAGAAGA ATCATATTTT CCGTCTGACT
    CAAGGGCTGC AAGAAGCTCT GGATCGGGCT GATCTGCTGA AGACAGAAAG GAGTGACCTG
    GAATATCAGC TGGAAAACAT TCAGGTTCTC TATTCTCATG AGAAGGTGAA GATGGAAGGC
    ACTATTTCCC AGCAAACCAA ACTCATCGAT TTTCTGCAAG CCAAAATGGA CCAGCCTGCC
    AAAAAGAAAA AGGGTTTATT TAGTCGACGG AAAGAGGACC CTGCTTTGCC CACACAGGTT
    CCTCTGCAGT ACAATGAGCT GAAGGTGGCT CTGGAGAAGG AGAAAGCTCG CTGCGCGGAG
    CTCGAGGAAG CCCTGCAGAA GACCCGCATC GAGCTCCGCT CCGCCCGGGA GGAAGCTGCC
    CACCGGAAAG CTGCAGACCA CCCACACCCA TCTACGCCGG CTACCGCCAG GCAGCAGATC
    GCCATGTCCG CCATTGTGCG GTCACCCGAG CACCAGCCCA GCGCCCTGAG CCTGCTCGCC
    CCGCCGTCCA GCCGCAGGAA GGAGTCTTCC ACTCCAGAGG AATTCAGCCG GCGTCTTAAG
    GAGCGCATGC ACCACAACAT CCCTCACCGG TTCAACGTGG GACTGAACAT GCGAGCCACG
    AAGTGCGCCG TGTGTCTGGA TACCGTGCAC TTCGGGCGCC AGGCATCCAA GTGTCTCGAA
    TGTCAGGTCA TGTGCCACCC CAAGTGCTCC ACATGCTTGC CAGCCACCTG TGGCCTGCCA
    GCCGAATACG CCACACACTT CACCGAGGCC TTCTGCCGCG ACAAAATGAA CTCCCCGGGT
    CTGCAGTCCA AGGAGCCCAG CAGCAGCCTG CACCTGGAAG GGTGGATGAA GGTGCCCAGG
    AATAACAAGC GAGGACAGCA AGGCTGGGAC AGGAAGTACA TCGTTCTGGA GGGCTCCAAA
    GTCCTCATTT ATGACAACGA AGCCAGAGAA GCTGGACAGA GGCCGGTGGA AGAATTTGAG
    CTGTGCCTTC CCGACGGGGA CGTGTCTATT CATGGCGCCG TTGGTGCTTC TGAACTTGCA
    AACACAGCCA AAGCAGATGT CCCCTATGTC CTGAAGATGG AGTCTCACCC CCATACCACC
    TGCTGGCCTG GGAGGACCCT CTACTTGCTG GCTCCCAGCT TTCCCGACAA GCAGCGCTGG
    GTCACCGCCT TAGAGTCAGT TGTGGCAGGT GGGAGAGTGT CTAGGGAAAA GGCGGAAGCC
    GACGCTAAAT TGCTCGGAAA CTCCCTGTTG AAACTGGAAG GTGATGACCG TCTGGACATG
    AACTGCACGC TGCCCTTCAG CGACCAGGTG GTGCTGGTGG GCACCGAGGA AGGGCTGTAT
    GCGCTGAATG TCTTGAAGAA CTCCCTCACC CACGTCCCAG GAATCGGAGC AGTCTTCCAG
    ATTTACATCA TCAAGGACCT GGAGAAGCTC CTCATGATCG CAGGAGAGGA GCGGGCCCTG
    TGTCTCGTGG ATGTGAAGAA GGTGAAGCAG TCCCTCGCGC AGTCTCACCT CCCCGCCCAG
    CCAGACATCT CGCCCAACAT CTTTGAAGCG GTCAAGGGCT GCCACTTGTT TGCTGCTGGC
    AAGATTGAGA GCGGGCTCTG CATCTGTGCA GCCATGCCCA GCAAAGTTGT CATTCTCCGC
    TACAATGAAA ACCTCAGCAA GTACTGCATT CGCAAAGAGA TCGAGACCTC GGAGCCCTGC
    AGCTGTATCC ACTTCACCAA CTACAGTATC CTCATCGGAA CCAATAAATT CTACGAAATC
    GACATGAAGC AGTACACGCT AGAGGAATTC CTGGATAAGA ACGACCATTC CTTGGCGCCT
    GCCGTGTTTG CCTCCTCTTC CAACAGCTTC CCCGTGTCCA TCATGCAGGT GAACGGCGCG
    GGGCAGCGGG AGGAGTTCCT GCTCTGTTTC CACGAATTCG GGGTGTTCGT GGATTCTTAT
    GGAAGACGCA GCCGCACAGA TGATCTCAAG TGGAGTCGCT TACCTCTGGC CTTTGCCTAC
    AGAGAACCCT ATCTGTTTGT GACTCACTTC AACTCACTCG AAGTAATTGA GATCCAGGCC
    CGCTCCTCTC TGGGGACCCC CGCCCGAGCA TATTTGGAAA TCCCCAACCC ACGCTACCTG
    GGCCCTGCAA TCTCCTCAGG AGCCATCTAC CTGGCGTCCT CATATCAGGA TAAGTTAAGG
    GTCATCTGCT GCAAAGGAAA CCTGGTGAAG GAGACCGGCA CCGACCACCA CCGTGGCCCA
    TCCACCTCCC GCAGCCCCAA CAAGCGAGGC CCGCCGACGT ACAACGAGCA CATCACCAAG
    CGCGTGGCCT CAAGCCCGGC GCCTCCCGAA GGCCCCAGCC ACCCTCGAGA GCCAAGCACA
    CCCCACCGCT ACCGCGAGGG GCGGACCGAG CTGCGCAGGG ACAAGTCTCC GGGCCGCCCC
    CTGGAGCGCG AGAAGTCCCC CGGCCGGATG CTGAGCACGC GGAGGGAGCG CTCCCCCGGG
    CGGCTGTTTG AGGACAGCAG CAGGGGCCGG CTGCCCGTGG GGGCCGTGAG GACCCCGCTG
    TCCCAGGTCA ACAAGGTCTG GGACCAGTCT TCAGTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015331020.1
    CDS263..6241
    Translation

    Target ORF information:

    RefSeq Version XM_015475534.1
    Organism Bos taurus(cattle)
    Definition Bos taurus citron rho-interacting serine/threonine kinase (CIT), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015475534.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    ATGTTGAAGT TCAAGTATGG AGCACGGAAT CTACTGGACG CTGGTACTGT TGAATCCATT 
    GCCAGCCGGG CCTCCAGGCT GAATCTTTTC TTCCAGGGGA AACCACCCTC CATGACTCAA
    CAGCAGATGT CTCTTCTTTC CCGAGAAGCG ATATTAGATG CCCTCTTTGT TCTCTTTAAA
    GAATGCAGTC AGCCAGCTCT GATGAAGATT AAGCACGTGA GCAACTTTGT CCAAAAGTGT
    AAGTGTGGGG AGAACTTTTT CTTGAAAATT GTCCTGCTTA GTGAGAATGT CATGGAATAT
    CAGCCTGGAG GGGATTTGCT GTCACTTTTG AATAGATATG AGGACCAATT AGATGAAAAT
    ATGATTCAGT TTTACCTAGC TGAACTGATT TTGGCTGTCC ACAGCGTTCA TCAGATGGGC
    TATGTACATC GAGACATCAA GCCCGAGAAC ATCCTCATTG ACCGAACGGG ACACATCAAG
    CTGGTGGATT TTGGATCAGC TGCTAAAATG AACTCAAATA AGATGGTAAA TGCCAAACTC
    CCGGTTGGTA CTCCAGATTA CATGGCCCCT GAAGTGTTGA CTGTGATGAA TGGGGATGGA
    AAAGGTGCCT ATAGCCTAGA CTGTGATTGG TGGTCAGTGG GAGTTATTGC TTATGAGATG
    GTTTATGGAA GATGCCCATT CACTGAAGGA ACCTCAGCCA AAACCTTCAA TAACATCATG
    AATTTCCAGC GGTTCTTGAA GTTTCCAGAT GACCCCAAAG TTAGCAGTGA ATTGCTTGAT
    CTAATCCAGA GTTTGTTGTG TGGCCAGAAA GAGAGACTGA AGTTTGAGGG CCTTTGCTGT
    CATCCTTTCT TCTCCAAAAT TGACTGGATT AACATTCGTA ACTCTCCTCC CCCCTTCGTT
    CCCACCCTCA AGTCTGATGA TGACACCTCC AATTTTGATG AACCAGAGAA GAATTCGTGG
    GTTTCATCCT CTCCGTGCCA GCTGAACCCC TCAGGTTTCT CGGGCGAAGA ACTGCCGTTT
    GTGGGGTTTT CGTATAGCAA GGCACTGGGA ATTCTTGGTA GATCTGAGCC TGTTGTATCA
    AGTCTGGACT CCCCTGCCAA GACTAGCTCC ATGGAAAAGA AACTCCTTAT CAAAAGCAAA
    GAGCTGCAGG ACTCCCAGGA CAAGTGTCAC AAGATGGAGC AGGAAATGAC CCGGTTACAT
    CGGAGAGTGT CAGAGGTGGA GGCTGTGCTT AGTCAGAAGG AGGTGGAGCT GAAGGCCTCT
    GAGACTCAGA GATCCCTCCT GGAGCAGGAC CTTGCTACCT ACATCACAGA ATGCAGTAGC
    TTAAAGCGAA GTTTGGAGCA AGCACGGATG GAGGTGTCCC AAGAGGACGA CAAAGCACTG
    CAGCTCCTCC ATGATATCAG AGAGCAGAGC CGGAAGCTCC AAGAAATCAA AGAGCAGGAG
    TACCAGGCTC AAGTGGAAGA AATGAGGTTG ATGATGAATC AGTTGGAAGA GGACCTGGTT
    TCGGCGAGAA GGCGGAGTGA CCTCTATGAA TCGGAGCTCA GAGAGTCTCG GCTGGCTGCT
    GAAGAGTTTA AGCGGAAAGC AACAGAATGT CAGCATAAAC TGATGAAGGC TAAAGATCAA
    GGGAAGCCTG AAGTGGGAGA ATATGCCAAA CTGGAGAAGA TCAATGCTGA GCAGCAGCTC
    AAAATTCAGG AGCTCCAAGA GAAGCTAGAA AAGGCTGTGA AAGCTAGCAC AGAGGCCACC
    GAGCTGCTGC AGAACATCCG TCAGGCGAAG GAGCGGGCCG AGCGTGAGCT GGAGAAGCTG
    CACAACCGTG AGGACTCCTC CGAGGGCATC AAGAAGAAGC TGGTGGAGGC CGAGGAACGC
    CGCCACTCTC TGGAGAACAA GGTAAAGAGG CTAGAGACCA TGGAGCGTAG AGAAAACAGA
    CTGAAGGATG ACATCCAGAC AAAGTCCCAA CAGATCCAGC AGATGGCTGA TAAAATTCTG
    GAGCTGGAGG AGAAGCACAG AGAAGCCCAG GTCTCAGCGC AGCATCTGGA GGTGCACCTG
    AAACAGAAAG AGCAGCACTA TGAAGAAAAA ATTAAAGTGT TGGACAATCA GATAAAGAAA
    GACCTAGCAG ACAAGGAGAC ACTGGAGAAC CTGATGCAAA GACACGAAGA GGAGGCCCAT
    GAGAAAGGCA AAATTCTCAG CGAGCAGAAG GCGATGATCA ATGCTATGGA TTCCAAGATC
    AGATCCCTGG AACAAAGGAT AGTGGAATTG TCGGAAGCCA ATAAACTTGC GGCCAACAGC
    AGTCTCTTTA CCCAGAGGAA CATGAAGGCC CAGGAAGAGA TGATTTCAGA ACTCAGGCAA
    CAGAAGTTTT ACCTGGAGAC GCAAGCTGGG AAATTGGAGG CCCAGAACCG GAAGCTGGAG
    GAGCAGCTGG AAAAAATAAG CCACCAAGAC CACAGTGACA AGAACCGTCT GCTGGAGCTG
    GAGACCAGGC TAAGGGAGGT CAGTCTAGAA CATGAAGAGC AGAAACTGGA GCTCAAACGC
    CAGCTCACAG AGCTGCAGCT CTCCCTGCAG GAGCGTGAAT CCCAGCTGAC AGCCCTGCAG
    GCAGCACGGG CGGCCCTGGA GAGCCAGCTC CGCCAGGCCA AGACGGAGCT GGAAGAGACG
    ACAGCAGAAG CAGAAGAAGA GATCCAGGCG CTTACGGCAC ATAGAGATGA AATCCAGCGC
    AAATTCGATG CCCTTCGTAA CAGCTGTACT GTGATCACGG ACTTGGAGGA GCAGCTAAAC
    CAGCTGACGG AGGACAATGC CGAGCTCAAC AACCAAAACT TCTACTTGTC TAAACAACTC
    GATGAGGCCT CTGGTGCCAA CGACGAGATT GTGCAGCTGC GAAGTGAAGT GGACCATCTC
    CGCCGCGAGA TCACTGAGAG GGAGATGCAG CTCACTAGCC AGAAGCAAAC GATGGAGGCG
    CTGAAGACCA CGTGCACAAT GCTGGAGGAG CAGGTCATGG ACCTGGAGGC CCTGAACGAC
    GAGCTGCTGG AGAAGGAGCG GCAGTGGGAG GCCTGGAGGA GTGTCCTTGG TGACGAGAAG
    TCCCAGTTTG AGTGTCGGGT TCGAGAGTTG CAGAGGATGC TGGACACCGA AAAGCAGAGC
    AGGGCGAGGG CCGACCAGAG GATCACTGAG TCGCGCCAGG TGGTCGAGCT GGCAGTAAAG
    GAGCACAAGG CCGAGATCCT GGCCTTGCAG CAGGCTCTCA AGGAGCAGAA GCTGAAGGCC
    GAGAGCCTCT CCGACAAGCT CAATGATCTG GAGAAGAAGC ACGCCATGCT TGAAATGAAT
    GCCCGAAGTT TACAACAGAA GCTGGAGACA GAACGAGAGC TCAAACAAAG GCTTCTGGAA
    GAGCAAGCCA AGTTACAGCA GCAGATGGAC CTGCAGAAGA ATCATATTTT CCGTCTGACT
    CAAGGGCTGC AAGAAGCTCT GGATCGGGCT GATCTGCTGA AGACAGAAAG GAGTGACCTG
    GAATATCAGC TGGAAAACAT TCAGGTTCTC TATTCTCATG AGAAGGTGAA GATGGAAGGC
    ACTATTTCCC AGCAAACCAA ACTCATCGAT TTTCTGCAAG CCAAAATGGA CCAGCCTGCC
    AAAAAGAAAA AGGGTTTATT TAGTCGACGG AAAGAGGACC CTGCTTTGCC CACACAGGTT
    CCTCTGCAGT ACAATGAGCT GAAGGTGGCT CTGGAGAAGG AGAAAGCTCG CTGCGCGGAG
    CTCGAGGAAG CCCTGCAGAA GACCCGCATC GAGCTCCGCT CCGCCCGGGA GGAAGCTGCC
    CACCGGAAAG CTGCAGACCA CCCACACCCA TCTACGCCGG CTACCGCCAG GCAGCAGATC
    GCCATGTCCG CCATTGTGCG GTCACCCGAG CACCAGCCCA GCGCCCTGAG CCTGCTCGCC
    CCGCCGTCCA GCCGCAGGAA GGAGTCTTCC ACTCCAGAGG AATTCAGCCG GCGTCTTAAG
    GAGCGCATGC ACCACAACAT CCCTCACCGG TTCAACGTGG GACTGAACAT GCGAGCCACG
    AAGTGCGCCG TGTGTCTGGA TACCGTGCAC TTCGGGCGCC AGGCATCCAA GTGTCTCGAA
    TGTCAGGTCA TGTGCCACCC CAAGTGCTCC ACATGCTTGC CAGCCACCTG TGGCCTGCCA
    GCCGAATACG CCACACACTT CACCGAGGCC TTCTGCCGCG ACAAAATGAA CTCCCCGGGT
    CTGCAGTCCA AGGAGCCCAG CAGCAGCCTG CACCTGGAAG GGTGGATGAA GGTGCCCAGG
    AATAACAAGC GAGGACAGCA AGGCTGGGAC AGGAAGTACA TCGTTCTGGA GGGCTCCAAA
    GTCCTCATTT ATGACAACGA AGCCAGAGAA GCTGGACAGA GGCCGGTGGA AGAATTTGAG
    CTGTGCCTTC CCGACGGGGA CGTGTCTATT CATGGCGCCG TTGGTGCTTC TGAACTTGCA
    AACACAGCCA AAGCAGATGT CCCCTATGTC CTGAAGATGG AGTCTCACCC CCATACCACC
    TGCTGGCCTG GGAGGACCCT CTACTTGCTG GCTCCCAGCT TTCCCGACAA GCAGCGCTGG
    GTCACCGCCT TAGAGTCAGT TGTGGCAGGT GGGAGAGTGT CTAGGGAAAA GGCGGAAGCC
    GACGCTAAAT TGCTCGGAAA CTCCCTGTTG AAACTGGAAG GTGATGACCG TCTGGACATG
    AACTGCACGC TGCCCTTCAG CGACCAGGTG GTGCTGGTGG GCACCGAGGA AGGGCTGTAT
    GCGCTGAATG TCTTGAAGAA CTCCCTCACC CACGTCCCAG GAATCGGAGC AGTCTTCCAG
    ATTTACATCA TCAAGGACCT GGAGAAGCTC CTCATGATCG CAGGAGAGGA GCGGGCCCTG
    TGTCTCGTGG ATGTGAAGAA GGTGAAGCAG TCCCTCGCGC AGTCTCACCT CCCCGCCCAG
    CCAGACATCT CGCCCAACAT CTTTGAAGCG GTCAAGGGCT GCCACTTGTT TGCTGCTGGC
    AAGATTGAGA GCGGGCTCTG CATCTGTGCA GCCATGCCCA GCAAAGTTGT CATTCTCCGC
    TACAATGAAA ACCTCAGCAA GTACTGCATT CGCAAAGAGA TCGAGACCTC GGAGCCCTGC
    AGCTGTATCC ACTTCACCAA CTACAGTATC CTCATCGGAA CCAATAAATT CTACGAAATC
    GACATGAAGC AGTACACGCT AGAGGAATTC CTGGATAAGA ACGACCATTC CTTGGCGCCT
    GCCGTGTTTG CCTCCTCTTC CAACAGCTTC CCCGTGTCCA TCATGCAGGT GAACGGCGCG
    GGGCAGCGGG AGGAGTTCCT GCTCTGTTTC CACGAATTCG GGGTGTTCGT GGATTCTTAT
    GGAAGACGCA GCCGCACAGA TGATCTCAAG TGGAGTCGCT TACCTCTGGC CTTTGCCTAC
    AGAGAACCCT ATCTGTTTGT GACTCACTTC AACTCACTCG AAGTAATTGA GATCCAGGCC
    CGCTCCTCTC TGGGGACCCC CGCCCGAGCA TATTTGGAAA TCCCCAACCC ACGCTACCTG
    GGCCCTGCAA TCTCCTCAGG AGCCATCTAC CTGGCGTCCT CATATCAGGA TAAGTTAAGG
    GTCATCTGCT GCAAAGGAAA CCTGGTGAAG GAGACCGGCA CCGACCACCA CCGTGGCCCA
    TCCACCTCCC GCAGCCCCAA CAAGCGAGGC CCGCCGACGT ACAACGAGCA CATCACCAAG
    CGCGTGGCCT CAAGCCCGGC GCCTCCCGAA GGCCCCAGCC ACCCTCGAGA GCCAAGCACA
    CCCCACCGCT ACCGCGAGGG GCGGACCGAG CTGCGCAGGG ACAAGTCTCC GGGCCGCCCC
    CTGGAGCGCG AGAAGTCCCC CGGCCGGATG CTGAGCACGC GGAGGGAGCG CTCCCCCGGG
    CGGCTGTTTG AGGACAGCAG CAGGGGCCGG CTGCCCGTGG GGGCCGTGAG GACCCCGCTG
    TCCCAGGTCA ACAAGGTCTG GGACCAGTCT TCAGTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa93236
    Clone ID Related Accession (Same CDS sequence) XM_015475534.1 , XM_015467110.1
    Accession Version XM_015467110.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5979bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product citron Rho-interacting kinase
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646371.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    ATGTTGAAGT TCAAGTATGG AGCACGGAAT CTACTGGACG CTGGTACTGT TGAATCCATT 
    GCCAGCCGGG CCTCCAGGCT GAATCTTTTC TTCCAGGGGA AACCACCCTC CATGACTCAA
    CAGCAGATGT CTCTTCTTTC CCGAGAAGCG ATATTAGATG CCCTCTTTGT TCTCTTTAAA
    GAATGCAGTC AGCCAGCTCT GATGAAGATT AAGCACGTGA GCAACTTTGT CCAAAAGTGT
    AAGTGTGGGG AGAACTTTTT CTTGAAAATT GTCCTGCTTA GTGAGAATGT CATGGAATAT
    CAGCCTGGAG GGGATTTGCT GTCACTTTTG AATAGATATG AGGACCAATT AGATGAAAAT
    ATGATTCAGT TTTACCTAGC TGAACTGATT TTGGCTGTCC ACAGCGTTCA TCAGATGGGC
    TATGTACATC GAGACATCAA GCCCGAGAAC ATCCTCATTG ACCGAACGGG ACACATCAAG
    CTGGTGGATT TTGGATCAGC TGCTAAAATG AACTCAAATA AGATGGTAAA TGCCAAACTC
    CCGGTTGGTA CTCCAGATTA CATGGCCCCT GAAGTGTTGA CTGTGATGAA TGGGGATGGA
    AAAGGTGCCT ATAGCCTAGA CTGTGATTGG TGGTCAGTGG GAGTTATTGC TTATGAGATG
    GTTTATGGAA GATGCCCATT CACTGAAGGA ACCTCAGCCA AAACCTTCAA TAACATCATG
    AATTTCCAGC GGTTCTTGAA GTTTCCAGAT GACCCCAAAG TTAGCAGTGA ATTGCTTGAT
    CTAATCCAGA GTTTGTTGTG TGGCCAGAAA GAGAGACTGA AGTTTGAGGG CCTTTGCTGT
    CATCCTTTCT TCTCCAAAAT TGACTGGATT AACATTCGTA ACTCTCCTCC CCCCTTCGTT
    CCCACCCTCA AGTCTGATGA TGACACCTCC AATTTTGATG AACCAGAGAA GAATTCGTGG
    GTTTCATCCT CTCCGTGCCA GCTGAACCCC TCAGGTTTCT CGGGCGAAGA ACTGCCGTTT
    GTGGGGTTTT CGTATAGCAA GGCACTGGGA ATTCTTGGTA GATCTGAGCC TGTTGTATCA
    AGTCTGGACT CCCCTGCCAA GACTAGCTCC ATGGAAAAGA AACTCCTTAT CAAAAGCAAA
    GAGCTGCAGG ACTCCCAGGA CAAGTGTCAC AAGATGGAGC AGGAAATGAC CCGGTTACAT
    CGGAGAGTGT CAGAGGTGGA GGCTGTGCTT AGTCAGAAGG AGGTGGAGCT GAAGGCCTCT
    GAGACTCAGA GATCCCTCCT GGAGCAGGAC CTTGCTACCT ACATCACAGA ATGCAGTAGC
    TTAAAGCGAA GTTTGGAGCA AGCACGGATG GAGGTGTCCC AAGAGGACGA CAAAGCACTG
    CAGCTCCTCC ATGATATCAG AGAGCAGAGC CGGAAGCTCC AAGAAATCAA AGAGCAGGAG
    TACCAGGCTC AAGTGGAAGA AATGAGGTTG ATGATGAATC AGTTGGAAGA GGACCTGGTT
    TCGGCGAGAA GGCGGAGTGA CCTCTATGAA TCGGAGCTCA GAGAGTCTCG GCTGGCTGCT
    GAAGAGTTTA AGCGGAAAGC AACAGAATGT CAGCATAAAC TGATGAAGGC TAAAGATCAA
    GGGAAGCCTG AAGTGGGAGA ATATGCCAAA CTGGAGAAGA TCAATGCTGA GCAGCAGCTC
    AAAATTCAGG AGCTCCAAGA GAAGCTAGAA AAGGCTGTGA AAGCTAGCAC AGAGGCCACC
    GAGCTGCTGC AGAACATCCG TCAGGCGAAG GAGCGGGCCG AGCGTGAGCT GGAGAAGCTG
    CACAACCGTG AGGACTCCTC CGAGGGCATC AAGAAGAAGC TGGTGGAGGC CGAGGAACGC
    CGCCACTCTC TGGAGAACAA GGTAAAGAGG CTAGAGACCA TGGAGCGTAG AGAAAACAGA
    CTGAAGGATG ACATCCAGAC AAAGTCCCAA CAGATCCAGC AGATGGCTGA TAAAATTCTG
    GAGCTGGAGG AGAAGCACAG AGAAGCCCAG GTCTCAGCGC AGCATCTGGA GGTGCACCTG
    AAACAGAAAG AGCAGCACTA TGAAGAAAAA ATTAAAGTGT TGGACAATCA GATAAAGAAA
    GACCTAGCAG ACAAGGAGAC ACTGGAGAAC CTGATGCAAA GACACGAAGA GGAGGCCCAT
    GAGAAAGGCA AAATTCTCAG CGAGCAGAAG GCGATGATCA ATGCTATGGA TTCCAAGATC
    AGATCCCTGG AACAAAGGAT AGTGGAATTG TCGGAAGCCA ATAAACTTGC GGCCAACAGC
    AGTCTCTTTA CCCAGAGGAA CATGAAGGCC CAGGAAGAGA TGATTTCAGA ACTCAGGCAA
    CAGAAGTTTT ACCTGGAGAC GCAAGCTGGG AAATTGGAGG CCCAGAACCG GAAGCTGGAG
    GAGCAGCTGG AAAAAATAAG CCACCAAGAC CACAGTGACA AGAACCGTCT GCTGGAGCTG
    GAGACCAGGC TAAGGGAGGT CAGTCTAGAA CATGAAGAGC AGAAACTGGA GCTCAAACGC
    CAGCTCACAG AGCTGCAGCT CTCCCTGCAG GAGCGTGAAT CCCAGCTGAC AGCCCTGCAG
    GCAGCACGGG CGGCCCTGGA GAGCCAGCTC CGCCAGGCCA AGACGGAGCT GGAAGAGACG
    ACAGCAGAAG CAGAAGAAGA GATCCAGGCG CTTACGGCAC ATAGAGATGA AATCCAGCGC
    AAATTCGATG CCCTTCGTAA CAGCTGTACT GTGATCACGG ACTTGGAGGA GCAGCTAAAC
    CAGCTGACGG AGGACAATGC CGAGCTCAAC AACCAAAACT TCTACTTGTC TAAACAACTC
    GATGAGGCCT CTGGTGCCAA CGACGAGATT GTGCAGCTGC GAAGTGAAGT GGACCATCTC
    CGCCGCGAGA TCACTGAGAG GGAGATGCAG CTCACTAGCC AGAAGCAAAC GATGGAGGCG
    CTGAAGACCA CGTGCACAAT GCTGGAGGAG CAGGTCATGG ACCTGGAGGC CCTGAACGAC
    GAGCTGCTGG AGAAGGAGCG GCAGTGGGAG GCCTGGAGGA GTGTCCTTGG TGACGAGAAG
    TCCCAGTTTG AGTGTCGGGT TCGAGAGTTG CAGAGGATGC TGGACACCGA AAAGCAGAGC
    AGGGCGAGGG CCGACCAGAG GATCACTGAG TCGCGCCAGG TGGTCGAGCT GGCAGTAAAG
    GAGCACAAGG CCGAGATCCT GGCCTTGCAG CAGGCTCTCA AGGAGCAGAA GCTGAAGGCC
    GAGAGCCTCT CCGACAAGCT CAATGATCTG GAGAAGAAGC ACGCCATGCT TGAAATGAAT
    GCCCGAAGTT TACAACAGAA GCTGGAGACA GAACGAGAGC TCAAACAAAG GCTTCTGGAA
    GAGCAAGCCA AGTTACAGCA GCAGATGGAC CTGCAGAAGA ATCATATTTT CCGTCTGACT
    CAAGGGCTGC AAGAAGCTCT GGATCGGGCT GATCTGCTGA AGACAGAAAG GAGTGACCTG
    GAATATCAGC TGGAAAACAT TCAGGTTCTC TATTCTCATG AGAAGGTGAA GATGGAAGGC
    ACTATTTCCC AGCAAACCAA ACTCATCGAT TTTCTGCAAG CCAAAATGGA CCAGCCTGCC
    AAAAAGAAAA AGGGTTTATT TAGTCGACGG AAAGAGGACC CTGCTTTGCC CACACAGGTT
    CCTCTGCAGT ACAATGAGCT GAAGGTGGCT CTGGAGAAGG AGAAAGCTCG CTGCGCGGAG
    CTCGAGGAAG CCCTGCAGAA GACCCGCATC GAGCTCCGCT CCGCCCGGGA GGAAGCTGCC
    CACCGGAAAG CTGCAGACCA CCCACACCCA TCTACGCCGG CTACCGCCAG GCAGCAGATC
    GCCATGTCCG CCATTGTGCG GTCACCCGAG CACCAGCCCA GCGCCCTGAG CCTGCTCGCC
    CCGCCGTCCA GCCGCAGGAA GGAGTCTTCC ACTCCAGAGG AATTCAGCCG GCGTCTTAAG
    GAGCGCATGC ACCACAACAT CCCTCACCGG TTCAACGTGG GACTGAACAT GCGAGCCACG
    AAGTGCGCCG TGTGTCTGGA TACCGTGCAC TTCGGGCGCC AGGCATCCAA GTGTCTCGAA
    TGTCAGGTCA TGTGCCACCC CAAGTGCTCC ACATGCTTGC CAGCCACCTG TGGCCTGCCA
    GCCGAATACG CCACACACTT CACCGAGGCC TTCTGCCGCG ACAAAATGAA CTCCCCGGGT
    CTGCAGTCCA AGGAGCCCAG CAGCAGCCTG CACCTGGAAG GGTGGATGAA GGTGCCCAGG
    AATAACAAGC GAGGACAGCA AGGCTGGGAC AGGAAGTACA TCGTTCTGGA GGGCTCCAAA
    GTCCTCATTT ATGACAACGA AGCCAGAGAA GCTGGACAGA GGCCGGTGGA AGAATTTGAG
    CTGTGCCTTC CCGACGGGGA CGTGTCTATT CATGGCGCCG TTGGTGCTTC TGAACTTGCA
    AACACAGCCA AAGCAGATGT CCCCTATGTC CTGAAGATGG AGTCTCACCC CCATACCACC
    TGCTGGCCTG GGAGGACCCT CTACTTGCTG GCTCCCAGCT TTCCCGACAA GCAGCGCTGG
    GTCACCGCCT TAGAGTCAGT TGTGGCAGGT GGGAGAGTGT CTAGGGAAAA GGCGGAAGCC
    GACGCTAAAT TGCTCGGAAA CTCCCTGTTG AAACTGGAAG GTGATGACCG TCTGGACATG
    AACTGCACGC TGCCCTTCAG CGACCAGGTG GTGCTGGTGG GCACCGAGGA AGGGCTGTAT
    GCGCTGAATG TCTTGAAGAA CTCCCTCACC CACGTCCCAG GAATCGGAGC AGTCTTCCAG
    ATTTACATCA TCAAGGACCT GGAGAAGCTC CTCATGATCG CAGGAGAGGA GCGGGCCCTG
    TGTCTCGTGG ATGTGAAGAA GGTGAAGCAG TCCCTCGCGC AGTCTCACCT CCCCGCCCAG
    CCAGACATCT CGCCCAACAT CTTTGAAGCG GTCAAGGGCT GCCACTTGTT TGCTGCTGGC
    AAGATTGAGA GCGGGCTCTG CATCTGTGCA GCCATGCCCA GCAAAGTTGT CATTCTCCGC
    TACAATGAAA ACCTCAGCAA GTACTGCATT CGCAAAGAGA TCGAGACCTC GGAGCCCTGC
    AGCTGTATCC ACTTCACCAA CTACAGTATC CTCATCGGAA CCAATAAATT CTACGAAATC
    GACATGAAGC AGTACACGCT AGAGGAATTC CTGGATAAGA ACGACCATTC CTTGGCGCCT
    GCCGTGTTTG CCTCCTCTTC CAACAGCTTC CCCGTGTCCA TCATGCAGGT GAACGGCGCG
    GGGCAGCGGG AGGAGTTCCT GCTCTGTTTC CACGAATTCG GGGTGTTCGT GGATTCTTAT
    GGAAGACGCA GCCGCACAGA TGATCTCAAG TGGAGTCGCT TACCTCTGGC CTTTGCCTAC
    AGAGAACCCT ATCTGTTTGT GACTCACTTC AACTCACTCG AAGTAATTGA GATCCAGGCC
    CGCTCCTCTC TGGGGACCCC CGCCCGAGCA TATTTGGAAA TCCCCAACCC ACGCTACCTG
    GGCCCTGCAA TCTCCTCAGG AGCCATCTAC CTGGCGTCCT CATATCAGGA TAAGTTAAGG
    GTCATCTGCT GCAAAGGAAA CCTGGTGAAG GAGACCGGCA CCGACCACCA CCGTGGCCCA
    TCCACCTCCC GCAGCCCCAA CAAGCGAGGC CCGCCGACGT ACAACGAGCA CATCACCAAG
    CGCGTGGCCT CAAGCCCGGC GCCTCCCGAA GGCCCCAGCC ACCCTCGAGA GCCAAGCACA
    CCCCACCGCT ACCGCGAGGG GCGGACCGAG CTGCGCAGGG ACAAGTCTCC GGGCCGCCCC
    CTGGAGCGCG AGAAGTCCCC CGGCCGGATG CTGAGCACGC GGAGGGAGCG CTCCCCCGGG
    CGGCTGTTTG AGGACAGCAG CAGGGGCCGG CTGCCCGTGG GGGCCGTGAG GACCCCGCTG
    TCCCAGGTCA ACAAGGTCTG GGACCAGTCT TCAGTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015322596.1
    CDS263..6241
    Translation

    Target ORF information:

    RefSeq Version XM_015467110.1
    Organism Bos taurus(cattle)
    Definition Bos taurus citron rho-interacting serine/threonine kinase (CIT), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015467110.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    ATGTTGAAGT TCAAGTATGG AGCACGGAAT CTACTGGACG CTGGTACTGT TGAATCCATT 
    GCCAGCCGGG CCTCCAGGCT GAATCTTTTC TTCCAGGGGA AACCACCCTC CATGACTCAA
    CAGCAGATGT CTCTTCTTTC CCGAGAAGCG ATATTAGATG CCCTCTTTGT TCTCTTTAAA
    GAATGCAGTC AGCCAGCTCT GATGAAGATT AAGCACGTGA GCAACTTTGT CCAAAAGTGT
    AAGTGTGGGG AGAACTTTTT CTTGAAAATT GTCCTGCTTA GTGAGAATGT CATGGAATAT
    CAGCCTGGAG GGGATTTGCT GTCACTTTTG AATAGATATG AGGACCAATT AGATGAAAAT
    ATGATTCAGT TTTACCTAGC TGAACTGATT TTGGCTGTCC ACAGCGTTCA TCAGATGGGC
    TATGTACATC GAGACATCAA GCCCGAGAAC ATCCTCATTG ACCGAACGGG ACACATCAAG
    CTGGTGGATT TTGGATCAGC TGCTAAAATG AACTCAAATA AGATGGTAAA TGCCAAACTC
    CCGGTTGGTA CTCCAGATTA CATGGCCCCT GAAGTGTTGA CTGTGATGAA TGGGGATGGA
    AAAGGTGCCT ATAGCCTAGA CTGTGATTGG TGGTCAGTGG GAGTTATTGC TTATGAGATG
    GTTTATGGAA GATGCCCATT CACTGAAGGA ACCTCAGCCA AAACCTTCAA TAACATCATG
    AATTTCCAGC GGTTCTTGAA GTTTCCAGAT GACCCCAAAG TTAGCAGTGA ATTGCTTGAT
    CTAATCCAGA GTTTGTTGTG TGGCCAGAAA GAGAGACTGA AGTTTGAGGG CCTTTGCTGT
    CATCCTTTCT TCTCCAAAAT TGACTGGATT AACATTCGTA ACTCTCCTCC CCCCTTCGTT
    CCCACCCTCA AGTCTGATGA TGACACCTCC AATTTTGATG AACCAGAGAA GAATTCGTGG
    GTTTCATCCT CTCCGTGCCA GCTGAACCCC TCAGGTTTCT CGGGCGAAGA ACTGCCGTTT
    GTGGGGTTTT CGTATAGCAA GGCACTGGGA ATTCTTGGTA GATCTGAGCC TGTTGTATCA
    AGTCTGGACT CCCCTGCCAA GACTAGCTCC ATGGAAAAGA AACTCCTTAT CAAAAGCAAA
    GAGCTGCAGG ACTCCCAGGA CAAGTGTCAC AAGATGGAGC AGGAAATGAC CCGGTTACAT
    CGGAGAGTGT CAGAGGTGGA GGCTGTGCTT AGTCAGAAGG AGGTGGAGCT GAAGGCCTCT
    GAGACTCAGA GATCCCTCCT GGAGCAGGAC CTTGCTACCT ACATCACAGA ATGCAGTAGC
    TTAAAGCGAA GTTTGGAGCA AGCACGGATG GAGGTGTCCC AAGAGGACGA CAAAGCACTG
    CAGCTCCTCC ATGATATCAG AGAGCAGAGC CGGAAGCTCC AAGAAATCAA AGAGCAGGAG
    TACCAGGCTC AAGTGGAAGA AATGAGGTTG ATGATGAATC AGTTGGAAGA GGACCTGGTT
    TCGGCGAGAA GGCGGAGTGA CCTCTATGAA TCGGAGCTCA GAGAGTCTCG GCTGGCTGCT
    GAAGAGTTTA AGCGGAAAGC AACAGAATGT CAGCATAAAC TGATGAAGGC TAAAGATCAA
    GGGAAGCCTG AAGTGGGAGA ATATGCCAAA CTGGAGAAGA TCAATGCTGA GCAGCAGCTC
    AAAATTCAGG AGCTCCAAGA GAAGCTAGAA AAGGCTGTGA AAGCTAGCAC AGAGGCCACC
    GAGCTGCTGC AGAACATCCG TCAGGCGAAG GAGCGGGCCG AGCGTGAGCT GGAGAAGCTG
    CACAACCGTG AGGACTCCTC CGAGGGCATC AAGAAGAAGC TGGTGGAGGC CGAGGAACGC
    CGCCACTCTC TGGAGAACAA GGTAAAGAGG CTAGAGACCA TGGAGCGTAG AGAAAACAGA
    CTGAAGGATG ACATCCAGAC AAAGTCCCAA CAGATCCAGC AGATGGCTGA TAAAATTCTG
    GAGCTGGAGG AGAAGCACAG AGAAGCCCAG GTCTCAGCGC AGCATCTGGA GGTGCACCTG
    AAACAGAAAG AGCAGCACTA TGAAGAAAAA ATTAAAGTGT TGGACAATCA GATAAAGAAA
    GACCTAGCAG ACAAGGAGAC ACTGGAGAAC CTGATGCAAA GACACGAAGA GGAGGCCCAT
    GAGAAAGGCA AAATTCTCAG CGAGCAGAAG GCGATGATCA ATGCTATGGA TTCCAAGATC
    AGATCCCTGG AACAAAGGAT AGTGGAATTG TCGGAAGCCA ATAAACTTGC GGCCAACAGC
    AGTCTCTTTA CCCAGAGGAA CATGAAGGCC CAGGAAGAGA TGATTTCAGA ACTCAGGCAA
    CAGAAGTTTT ACCTGGAGAC GCAAGCTGGG AAATTGGAGG CCCAGAACCG GAAGCTGGAG
    GAGCAGCTGG AAAAAATAAG CCACCAAGAC CACAGTGACA AGAACCGTCT GCTGGAGCTG
    GAGACCAGGC TAAGGGAGGT CAGTCTAGAA CATGAAGAGC AGAAACTGGA GCTCAAACGC
    CAGCTCACAG AGCTGCAGCT CTCCCTGCAG GAGCGTGAAT CCCAGCTGAC AGCCCTGCAG
    GCAGCACGGG CGGCCCTGGA GAGCCAGCTC CGCCAGGCCA AGACGGAGCT GGAAGAGACG
    ACAGCAGAAG CAGAAGAAGA GATCCAGGCG CTTACGGCAC ATAGAGATGA AATCCAGCGC
    AAATTCGATG CCCTTCGTAA CAGCTGTACT GTGATCACGG ACTTGGAGGA GCAGCTAAAC
    CAGCTGACGG AGGACAATGC CGAGCTCAAC AACCAAAACT TCTACTTGTC TAAACAACTC
    GATGAGGCCT CTGGTGCCAA CGACGAGATT GTGCAGCTGC GAAGTGAAGT GGACCATCTC
    CGCCGCGAGA TCACTGAGAG GGAGATGCAG CTCACTAGCC AGAAGCAAAC GATGGAGGCG
    CTGAAGACCA CGTGCACAAT GCTGGAGGAG CAGGTCATGG ACCTGGAGGC CCTGAACGAC
    GAGCTGCTGG AGAAGGAGCG GCAGTGGGAG GCCTGGAGGA GTGTCCTTGG TGACGAGAAG
    TCCCAGTTTG AGTGTCGGGT TCGAGAGTTG CAGAGGATGC TGGACACCGA AAAGCAGAGC
    AGGGCGAGGG CCGACCAGAG GATCACTGAG TCGCGCCAGG TGGTCGAGCT GGCAGTAAAG
    GAGCACAAGG CCGAGATCCT GGCCTTGCAG CAGGCTCTCA AGGAGCAGAA GCTGAAGGCC
    GAGAGCCTCT CCGACAAGCT CAATGATCTG GAGAAGAAGC ACGCCATGCT TGAAATGAAT
    GCCCGAAGTT TACAACAGAA GCTGGAGACA GAACGAGAGC TCAAACAAAG GCTTCTGGAA
    GAGCAAGCCA AGTTACAGCA GCAGATGGAC CTGCAGAAGA ATCATATTTT CCGTCTGACT
    CAAGGGCTGC AAGAAGCTCT GGATCGGGCT GATCTGCTGA AGACAGAAAG GAGTGACCTG
    GAATATCAGC TGGAAAACAT TCAGGTTCTC TATTCTCATG AGAAGGTGAA GATGGAAGGC
    ACTATTTCCC AGCAAACCAA ACTCATCGAT TTTCTGCAAG CCAAAATGGA CCAGCCTGCC
    AAAAAGAAAA AGGGTTTATT TAGTCGACGG AAAGAGGACC CTGCTTTGCC CACACAGGTT
    CCTCTGCAGT ACAATGAGCT GAAGGTGGCT CTGGAGAAGG AGAAAGCTCG CTGCGCGGAG
    CTCGAGGAAG CCCTGCAGAA GACCCGCATC GAGCTCCGCT CCGCCCGGGA GGAAGCTGCC
    CACCGGAAAG CTGCAGACCA CCCACACCCA TCTACGCCGG CTACCGCCAG GCAGCAGATC
    GCCATGTCCG CCATTGTGCG GTCACCCGAG CACCAGCCCA GCGCCCTGAG CCTGCTCGCC
    CCGCCGTCCA GCCGCAGGAA GGAGTCTTCC ACTCCAGAGG AATTCAGCCG GCGTCTTAAG
    GAGCGCATGC ACCACAACAT CCCTCACCGG TTCAACGTGG GACTGAACAT GCGAGCCACG
    AAGTGCGCCG TGTGTCTGGA TACCGTGCAC TTCGGGCGCC AGGCATCCAA GTGTCTCGAA
    TGTCAGGTCA TGTGCCACCC CAAGTGCTCC ACATGCTTGC CAGCCACCTG TGGCCTGCCA
    GCCGAATACG CCACACACTT CACCGAGGCC TTCTGCCGCG ACAAAATGAA CTCCCCGGGT
    CTGCAGTCCA AGGAGCCCAG CAGCAGCCTG CACCTGGAAG GGTGGATGAA GGTGCCCAGG
    AATAACAAGC GAGGACAGCA AGGCTGGGAC AGGAAGTACA TCGTTCTGGA GGGCTCCAAA
    GTCCTCATTT ATGACAACGA AGCCAGAGAA GCTGGACAGA GGCCGGTGGA AGAATTTGAG
    CTGTGCCTTC CCGACGGGGA CGTGTCTATT CATGGCGCCG TTGGTGCTTC TGAACTTGCA
    AACACAGCCA AAGCAGATGT CCCCTATGTC CTGAAGATGG AGTCTCACCC CCATACCACC
    TGCTGGCCTG GGAGGACCCT CTACTTGCTG GCTCCCAGCT TTCCCGACAA GCAGCGCTGG
    GTCACCGCCT TAGAGTCAGT TGTGGCAGGT GGGAGAGTGT CTAGGGAAAA GGCGGAAGCC
    GACGCTAAAT TGCTCGGAAA CTCCCTGTTG AAACTGGAAG GTGATGACCG TCTGGACATG
    AACTGCACGC TGCCCTTCAG CGACCAGGTG GTGCTGGTGG GCACCGAGGA AGGGCTGTAT
    GCGCTGAATG TCTTGAAGAA CTCCCTCACC CACGTCCCAG GAATCGGAGC AGTCTTCCAG
    ATTTACATCA TCAAGGACCT GGAGAAGCTC CTCATGATCG CAGGAGAGGA GCGGGCCCTG
    TGTCTCGTGG ATGTGAAGAA GGTGAAGCAG TCCCTCGCGC AGTCTCACCT CCCCGCCCAG
    CCAGACATCT CGCCCAACAT CTTTGAAGCG GTCAAGGGCT GCCACTTGTT TGCTGCTGGC
    AAGATTGAGA GCGGGCTCTG CATCTGTGCA GCCATGCCCA GCAAAGTTGT CATTCTCCGC
    TACAATGAAA ACCTCAGCAA GTACTGCATT CGCAAAGAGA TCGAGACCTC GGAGCCCTGC
    AGCTGTATCC ACTTCACCAA CTACAGTATC CTCATCGGAA CCAATAAATT CTACGAAATC
    GACATGAAGC AGTACACGCT AGAGGAATTC CTGGATAAGA ACGACCATTC CTTGGCGCCT
    GCCGTGTTTG CCTCCTCTTC CAACAGCTTC CCCGTGTCCA TCATGCAGGT GAACGGCGCG
    GGGCAGCGGG AGGAGTTCCT GCTCTGTTTC CACGAATTCG GGGTGTTCGT GGATTCTTAT
    GGAAGACGCA GCCGCACAGA TGATCTCAAG TGGAGTCGCT TACCTCTGGC CTTTGCCTAC
    AGAGAACCCT ATCTGTTTGT GACTCACTTC AACTCACTCG AAGTAATTGA GATCCAGGCC
    CGCTCCTCTC TGGGGACCCC CGCCCGAGCA TATTTGGAAA TCCCCAACCC ACGCTACCTG
    GGCCCTGCAA TCTCCTCAGG AGCCATCTAC CTGGCGTCCT CATATCAGGA TAAGTTAAGG
    GTCATCTGCT GCAAAGGAAA CCTGGTGAAG GAGACCGGCA CCGACCACCA CCGTGGCCCA
    TCCACCTCCC GCAGCCCCAA CAAGCGAGGC CCGCCGACGT ACAACGAGCA CATCACCAAG
    CGCGTGGCCT CAAGCCCGGC GCCTCCCGAA GGCCCCAGCC ACCCTCGAGA GCCAAGCACA
    CCCCACCGCT ACCGCGAGGG GCGGACCGAG CTGCGCAGGG ACAAGTCTCC GGGCCGCCCC
    CTGGAGCGCG AGAAGTCCCC CGGCCGGATG CTGAGCACGC GGAGGGAGCG CTCCCCCGGG
    CGGCTGTTTG AGGACAGCAG CAGGGGCCGG CTGCCCGTGG GGGCCGTGAG GACCCCGCTG
    TCCCAGGTCA ACAAGGTCTG GGACCAGTCT TCAGTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa224622
    Clone ID Related Accession (Same CDS sequence) XM_024977733.1 , XM_024977734.1
    Accession Version XM_024977733.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6252bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product citron Rho-interacting kinase isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGTTGAAGT TCAAGTATGG AGCACGGAAT CTACTGGACG CTGGTACTGT TGAATCCATT 
    GCCAGCCGGG CCTCCAGGCT GAATCTTTTC TTCCAGGGGA AACCACCCTC CATGACTCAA
    CAGCAGATGT CTCTTCTTTC CCGAGAAGCG ATATTAGATG CCCTCTTTGT TCTCTTTAAA
    GAATGCAGTC AGCCAGCTCT GATGAAGATT AAGCACGTGA GCAACTTTGT CCAAAAGTAT
    TCCGATATCA TAGCTGAGTT GCAAGAGCTC CAGCCTTCAG TGAAGGACTT TGAAGTGAAA
    AGTGTCGTAG GCTGCGGTCA CTTTGCTGAA GTACAGGTGG TCAGAGAGAA AGCGACTGGG
    GATGTCTATG CCATGAAAGT CATGAAGAAG AAGGCCTTGT TGGCCCAGGA GCAGGTCTCA
    TATTTTGAGG AAGAACAGAA CATATTATCT CAGAGTACAA GTCCTTGGAT CCCCCAGTTG
    CAGTATGCCT TTCAGGACAA AAATAACCTT TATCTGGTCA TGGAATATCA GCCTGGAGGG
    GATTTGCTGT CACTTTTGAA TAGATATGAG GACCAATTAG ATGAAAATAT GATTCAGTTT
    TACCTAGCTG AACTGATTTT GGCTGTCCAC AGCGTTCATC AGATGGGCTA TGTACATCGA
    GACATCAAGC CCGAGAACAT CCTCATTGAC CGAACGGGAC ACATCAAGCT GGTGGATTTT
    GGATCAGCTG CTAAAATGAA CTCAAATAAG ATGGTAAATG CCAAACTCCC GGTTGGTACT
    CCAGATTACA TGGCCCCTGA AGTGTTGACT GTGATGAATG GGGATGGAAA AGGTGCCTAT
    AGCCTAGACT GTGATTGGTG GTCAGTGGGA GTTATTGCTT ATGAGATGGT TTATGGAAGA
    TGCCCATTCA CTGAAGGAAC CTCAGCCAAA ACCTTCAATA ACATCATGAA TTTCCAGCGG
    TTCTTGAAGT TTCCAGATGA CCCCAAAGTT AGCAGTGAAT TGCTTGATCT AATCCAGAGT
    TTGTTGTGTG GCCAGAAAGA GAGACTGAAG TTTGAGGGCC TTTGCTGTCA TCCTTTCTTC
    TCCAAAATTG ACTGGATTAA CATTCGTAAC TCTCCTCCCC CCTTCGTTCC CACCCTCAAG
    TCTGATGATG ACACCTCCAA TTTTGATGAA CCAGAGAAGA ATTCGTGGGT TTCATCCTCT
    CCGTGCCAGC TGAACCCCTC AGGTTTCTCG GGCGAAGAAC TGCCGTTTGT GGGGTTTTCG
    TATAGCAAGG CACTGGGAAT TCTTGGTAGA TCTGAGCCTG TTGTATCAAG TCTGGACTCC
    CCTGCCAAGA CTAGCTCCAT GGAAAAGAAA CTCCTTATCA AAAGCAAAGA GCTGCAGGAC
    TCCCAGGACA AGTGTCACAA GATGGAGCAG GAAATGACCC GGTTACATCG GAGAGTGTCA
    GAGGTGGAGG CTGTGCTTAG TCAGAAGGAG GTGGAGCTGA AGGCCTCTGA GACTCAGAGA
    TCCCTCCTGG AGCAGGACCT TGCTACCTAC ATCACAGAAT GCAGTAGCTT AAAGCGAAGT
    TTGGAGCAAG CACGGATGGA GGTGTCCCAA GAGGACGACA AAGCACTGCA GCTCCTCCAT
    GATATCAGAG AGCAGAGCCG GAAGCTCCAA GAAATCAAAG AGCAGGAGTA CCAGGCTCAA
    GTGGAAGAAA TGAGGTTGAT GATGAATCAG TTGGAAGAGG ACCTGGTTTC GGCGAGAAGG
    CGGAGTGACC TCTATGAATC GGAGCTCAGA GAGTCTCGGC TGGCTGCTGA AGAGTTTAAG
    CGGAAAGCAA CAGAATGTCA GCATAAACTG ATGAAGGCTA AAGATCAAGG GAAGCCTGAA
    GTGGGAGAAT ATGCCAAACT GGAGAAGATC AATGCTGAGC AGCAGCTCAA AATTCAGGAG
    CTCCAAGAGA AGCTAGAAAA GGCTGTGAAA GCTAGCACAG AGGCCACCGA GCTGCTGCAG
    AACATCCGTC AGGCGAAGGA GCGGGCCGAG CGTGAGCTGG AGAAGCTGCA CAACCGTGAG
    GACTCCTCCG AGGGCATCAA GAAGAAGCTG GTGGAGGCCG AGGAACGCCG CCACTCTCTG
    GAGAACAAGG TAAAGAGGCT AGAGACCATG GAGCGTAGAG AAAACAGACT GAAGGATGAC
    ATCCAGACAA AGTCCCAACA GATCCAGCAG ATGGCTGATA AAATTCTGGA GCTGGAGGAG
    AAGCACAGAG AAGCCCAGGT CTCAGCGCAG CATCTGGAGG TGCACCTGAA ACAGAAAGAG
    CAGCACTATG AAGAAAAAAT TAAAGTGTTG GACAATCAGA TAAAGAAAGA CCTAGCAGAC
    AAGGAGACAC TGGAGAACCT GATGCAAAGA CACGAAGAGG AGGCCCATGA GAAAGGCAAA
    ATTCTCAGCG AGCAGAAGGC GATGATCAAT GCTATGGATT CCAAGATCAG ATCCCTGGAA
    CAAAGGATAG TGGAATTGTC GGAAGCCAAT AAACTTGCGG CCAACAGCAG TCTCTTTACC
    CAGAGGAACA TGAAGGCCCA GGAAGAGATG ATTTCAGAAC TCAGGCAACA GAAGTTTTAC
    CTGGAGACGC AAGCTGGGAA ATTGGAGGCC CAGAACCGGA AGCTGGAGGA GCAGCTGGAA
    AAAATAAGCC ACCAAGACCA CAGTGACAAG AACCGTCTGC TGGAGCTGGA GACCAGGCTA
    AGGGAGGTCA GTCTAGAACA TGAAGAGCAG AAACTGGAGC TCAAACGCCA GCTCACAGAG
    CTGCAGCTCT CCCTGCAGGA GCGTGAATCC CAGCTGACAG CCCTGCAGGC AGCACGGGCG
    GCCCTGGAGA GCCAGCTCCG CCAGGCCAAG ACGGAGCTGG AAGAGACGAC AGCAGAAGCA
    GAAGAAGAGA TCCAGGCGCT TACGGCACAT AGAGATGAAA TCCAGCGCAA ATTCGATGCC
    CTTCGTAACA GCTGTACTGT GATCACGGAC TTGGAGGAGC AGCTAAACCA GCTGACGGAG
    GACAATGCCG AGCTCAACAA CCAAAACTTC TACTTGTCTA AACAACTCGA TGAGGCCTCT
    GGTGCCAACG ACGAGATTGT GCAGCTGCGA AGTGAAGTGG ACCATCTCCG CCGCGAGATC
    ACTGAGAGGG AGATGCAGCT CACTAGCCAG AAGCAAACGA TGGAGGCGCT GAAGACCACG
    TGCACAATGC TGGAGGAGCA GGTCATGGAC CTGGAGGCCC TGAACGACGA GCTGCTGGAG
    AAGGAGCGGC AGTGGGAGGC CTGGAGGAGT GTCCTTGGTG ACGAGAAGTC CCAGTTTGAG
    TGTCGGGTTC GAGAGTTGCA GAGGATGCTG GACACCGAAA AGCAGAGCAG GGCGAGGGCC
    GACCAGAGGA TCACTGAGTC GCGCCAGGTG GTCGAGCTGG CAGTAAAGGA GCACAAGGCC
    GAGATCCTGG CCTTGCAGCA GGCTCTCAAG GAGCAGAAGC TGAAGGCCGA GAGCCTCTCC
    GACAAGCTCA ATGATCTGGA GAAGAAGCAC GCCATGCTTG AAATGAATGC CCGAAGTTTA
    CAACAGAAGC TGGAGACAGA ACGAGAGCTC AAACAAAGGC TTCTGGAAGA GCAAGCCAAG
    TTACAGCAGC AGATGGACCT GCAGAAGAAT CATATTTTCC GTCTGACTCA AGGGCTGCAA
    GAAGCTCTGG ATCGGGCTGA TCTGCTGAAG ACAGAAAGGA GTGACCTGGA ATATCAGCTG
    GAAAACATTC AGGTTCTCTA TTCTCATGAG AAGGTGAAGA TGGAAGGCAC TATTTCCCAG
    CAAACCAAAC TCATCGATTT TCTGCAAGCC AAAATGGACC AGCCTGCCAA AAAGAAAAAG
    GGTTTATTTA GTCGACGGAA AGAGGACCCT GCTTTGCCCA CACAGGTTCC TCTGCAGTAC
    AATGAGCTGA AGGTGGCTCT GGAGAAGGAG AAAGCTCGCT GCGCGGAGCT CGAGGAAGCC
    CTGCAGAAGA CCCGCATCGA GCTCCGCTCC GCCCGGGAGG AAGCTGCCCA CCGGAAAGCT
    GCAGACCACC CACACCCATC TACGCCGGCT ACCGCCAGGC AGCAGATCGC CATGTCCGCC
    ATTGTGCGGT CACCCGAGCA CCAGCCCAGC GCCCTGAGCC TGCTCGCCCC GCCGTCCAGC
    CGCAGGAAGG AGTCTTCCAC TCCAGAGGAA TTCAGCCGGC GTCTTAAGGA GCGCATGCAC
    CACAACATCC CTCACCGGTT CAACGTGGGA CTGAACATGC GAGCCACGAA GTGCGCCGTG
    TGTCTGGATA CCGTGCACTT CGGGCGCCAG GCATCCAAGT GTCTCGAATG TCAGGTCATG
    TGCCACCCCA AGTGCTCCAC ATGCTTGCCA GCCACCTGTG GCCTGCCAGC CGAATACGCC
    ACACACTTCA CCGAGGCCTT CTGCCGCGAC AAAATGAACT CCCCGGGTCT GCAGTCCAAG
    GAGCCCAGCA GCAGCCTGCA CCTGGAAGGG TGGATGAAGG TGCCCAGGAA TAACAAGCGA
    GGACAGCAAG GCTGGGACAG GAAGTACATC GTTCTGGAGG GCTCCAAAGT CCTCATTTAT
    GACAACGAAG CCAGAGAAGC TGGACAGAGG CCGGTGGAAG AATTTGAGCT GTGCCTTCCC
    GACGGGGACG TGTCTATTCA TGGCGCCGTT GGTGCTTCTG AACTTGCAAA CACAGCCAAA
    GCAGATGTCC CCTATGTCCT GAAGATGGAG TCTCACCCCC ATACCACCTG CTGGCCTGGG
    AGGACCCTCT ACTTGCTGGC TCCCAGCTTT CCCGACAAGC AGCGCTGGGT CACCGCCTTA
    GAGTCAGTTG TGGCAGGTGG GAGAGTGTCT AGGGAAAAGG CGGAAGCCGA CGCTGGCCGT
    GACTGTACTT CCTACGAGCC TCTGCCCGCG TGGGCTCAGA AATTGCTCGG AAACTCCCTG
    TTGAAACTGG AAGGTGATGA CCGTCTGGAC ATGAACTGCA CGCTGCCCTT CAGCGACCAG
    GTGGTGCTGG TGGGCACCGA GGAAGGGCTG TATGCGCTGA ATGTCTTGAA GAACTCCCTC
    ACCCACGTCC CAGGAATCGG AGCAGTCTTC CAGATTTACA TCATCAAGGA CCTGGAGAAG
    CTCCTCATGA TCGCAGGAGA GGAGCGGGCC CTGTGTCTCG TGGATGTGAA GAAGGTGAAG
    CAGTCCCTCG CGCAGTCTCA CCTCCCCGCC CAGCCAGACA TCTCGCCCAA CATCTTTGAA
    GCGGTCAAGG GCTGCCACTT GTTTGCTGCT GGCAAGATTG AGAGCGGGCT CTGCATCTGT
    GCAGCCATGC CCAGCAAAGT TGTCATTCTC CGCTACAATG AAAACCTCAG CAAGTACTGC
    ATTCGCAAAG AGATCGAGAC CTCGGAGCCC TGCAGCTGTA TCCACTTCAC CAACTACAGT
    ATCCTCATCG GAACCAATAA ATTCTACGAA ATCGACATGA AGCAGTACAC GCTAGAGGAA
    TTCCTGGATA AGAACGACCA TTCCTTGGCG CCTGCCGTGT TTGCCTCCTC TTCCAACAGC
    TTCCCCGTGT CCATCATGCA GGTGAACGGC GCGGGGCAGC GGGAGGAGTT CCTGCTCTGT
    TTCCACGAAT TCGGGGTGTT CGTGGATTCT TATGGAAGAC GCAGCCGCAC AGATGATCTC
    AAGTGGAGTC GCTTACCTCT GGCCTTTGCC TACAGAGAAC CCTATCTGTT TGTGACTCAC
    TTCAACTCAC TCGAAGTAAT TGAGATCCAG GCCCGCTCCT CTCTGGGGAC CCCCGCCCGA
    GCATATTTGG AAATCCCCAA CCCACGCTAC CTGGGCCCTG CAATCTCCTC AGGAGCCATC
    TACCTGGCGT CCTCATATCA GGATAAGTTA AGGGTCATCT GCTGCAAAGG AAACCTGGTG
    AAGGAGACCG GCACCGACCA CCACCGTGGC CCATCCACCT CCCGCAGCCC CAACAAGCGA
    GGCCCGCCGA CGTACAACGA GCACATCACC AAGCGCGTGG CCTCAAGCCC GGCGCCTCCC
    GAAGGCCCCA GCCACCCTCG AGAGCCAAGC ACACCCCACC GCTACCGCGA GGGGCGGACC
    GAGCTGCGCA GGGACAAGTC TCCGGGCCGC CCCCTGGAGC GCGAGAAGTC CCCCGGCCGG
    ATGCTGAGCA CGCGGAGGGA GCGCTCCCCC GGGCGGCTGT TTGAGGACAG CAGCAGGGGC
    CGGCTGCCCG TGGGGGCCGT GAGGACCCCG CTGTCCCAGG TCAACAAGGT CTGGGACCAG
    TCTTCAGTAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024833501.1
    CDS113..6364
    Translation

    Target ORF information:

    RefSeq Version XM_024977733.1
    Organism Bos taurus(cattle)
    Definition Bos taurus citron rho-interacting serine/threonine kinase (CIT), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024977733.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGTTGAAGT TCAAGTATGG AGCACGGAAT CTACTGGACG CTGGTACTGT TGAATCCATT 
    GCCAGCCGGG CCTCCAGGCT GAATCTTTTC TTCCAGGGGA AACCACCCTC CATGACTCAA
    CAGCAGATGT CTCTTCTTTC CCGAGAAGCG ATATTAGATG CCCTCTTTGT TCTCTTTAAA
    GAATGCAGTC AGCCAGCTCT GATGAAGATT AAGCACGTGA GCAACTTTGT CCAAAAGTAT
    TCCGATATCA TAGCTGAGTT GCAAGAGCTC CAGCCTTCAG TGAAGGACTT TGAAGTGAAA
    AGTGTCGTAG GCTGCGGTCA CTTTGCTGAA GTACAGGTGG TCAGAGAGAA AGCGACTGGG
    GATGTCTATG CCATGAAAGT CATGAAGAAG AAGGCCTTGT TGGCCCAGGA GCAGGTCTCA
    TATTTTGAGG AAGAACAGAA CATATTATCT CAGAGTACAA GTCCTTGGAT CCCCCAGTTG
    CAGTATGCCT TTCAGGACAA AAATAACCTT TATCTGGTCA TGGAATATCA GCCTGGAGGG
    GATTTGCTGT CACTTTTGAA TAGATATGAG GACCAATTAG ATGAAAATAT GATTCAGTTT
    TACCTAGCTG AACTGATTTT GGCTGTCCAC AGCGTTCATC AGATGGGCTA TGTACATCGA
    GACATCAAGC CCGAGAACAT CCTCATTGAC CGAACGGGAC ACATCAAGCT GGTGGATTTT
    GGATCAGCTG CTAAAATGAA CTCAAATAAG ATGGTAAATG CCAAACTCCC GGTTGGTACT
    CCAGATTACA TGGCCCCTGA AGTGTTGACT GTGATGAATG GGGATGGAAA AGGTGCCTAT
    AGCCTAGACT GTGATTGGTG GTCAGTGGGA GTTATTGCTT ATGAGATGGT TTATGGAAGA
    TGCCCATTCA CTGAAGGAAC CTCAGCCAAA ACCTTCAATA ACATCATGAA TTTCCAGCGG
    TTCTTGAAGT TTCCAGATGA CCCCAAAGTT AGCAGTGAAT TGCTTGATCT AATCCAGAGT
    TTGTTGTGTG GCCAGAAAGA GAGACTGAAG TTTGAGGGCC TTTGCTGTCA TCCTTTCTTC
    TCCAAAATTG ACTGGATTAA CATTCGTAAC TCTCCTCCCC CCTTCGTTCC CACCCTCAAG
    TCTGATGATG ACACCTCCAA TTTTGATGAA CCAGAGAAGA ATTCGTGGGT TTCATCCTCT
    CCGTGCCAGC TGAACCCCTC AGGTTTCTCG GGCGAAGAAC TGCCGTTTGT GGGGTTTTCG
    TATAGCAAGG CACTGGGAAT TCTTGGTAGA TCTGAGCCTG TTGTATCAAG TCTGGACTCC
    CCTGCCAAGA CTAGCTCCAT GGAAAAGAAA CTCCTTATCA AAAGCAAAGA GCTGCAGGAC
    TCCCAGGACA AGTGTCACAA GATGGAGCAG GAAATGACCC GGTTACATCG GAGAGTGTCA
    GAGGTGGAGG CTGTGCTTAG TCAGAAGGAG GTGGAGCTGA AGGCCTCTGA GACTCAGAGA
    TCCCTCCTGG AGCAGGACCT TGCTACCTAC ATCACAGAAT GCAGTAGCTT AAAGCGAAGT
    TTGGAGCAAG CACGGATGGA GGTGTCCCAA GAGGACGACA AAGCACTGCA GCTCCTCCAT
    GATATCAGAG AGCAGAGCCG GAAGCTCCAA GAAATCAAAG AGCAGGAGTA CCAGGCTCAA
    GTGGAAGAAA TGAGGTTGAT GATGAATCAG TTGGAAGAGG ACCTGGTTTC GGCGAGAAGG
    CGGAGTGACC TCTATGAATC GGAGCTCAGA GAGTCTCGGC TGGCTGCTGA AGAGTTTAAG
    CGGAAAGCAA CAGAATGTCA GCATAAACTG ATGAAGGCTA AAGATCAAGG GAAGCCTGAA
    GTGGGAGAAT ATGCCAAACT GGAGAAGATC AATGCTGAGC AGCAGCTCAA AATTCAGGAG
    CTCCAAGAGA AGCTAGAAAA GGCTGTGAAA GCTAGCACAG AGGCCACCGA GCTGCTGCAG
    AACATCCGTC AGGCGAAGGA GCGGGCCGAG CGTGAGCTGG AGAAGCTGCA CAACCGTGAG
    GACTCCTCCG AGGGCATCAA GAAGAAGCTG GTGGAGGCCG AGGAACGCCG CCACTCTCTG
    GAGAACAAGG TAAAGAGGCT AGAGACCATG GAGCGTAGAG AAAACAGACT GAAGGATGAC
    ATCCAGACAA AGTCCCAACA GATCCAGCAG ATGGCTGATA AAATTCTGGA GCTGGAGGAG
    AAGCACAGAG AAGCCCAGGT CTCAGCGCAG CATCTGGAGG TGCACCTGAA ACAGAAAGAG
    CAGCACTATG AAGAAAAAAT TAAAGTGTTG GACAATCAGA TAAAGAAAGA CCTAGCAGAC
    AAGGAGACAC TGGAGAACCT GATGCAAAGA CACGAAGAGG AGGCCCATGA GAAAGGCAAA
    ATTCTCAGCG AGCAGAAGGC GATGATCAAT GCTATGGATT CCAAGATCAG ATCCCTGGAA
    CAAAGGATAG TGGAATTGTC GGAAGCCAAT AAACTTGCGG CCAACAGCAG TCTCTTTACC
    CAGAGGAACA TGAAGGCCCA GGAAGAGATG ATTTCAGAAC TCAGGCAACA GAAGTTTTAC
    CTGGAGACGC AAGCTGGGAA ATTGGAGGCC CAGAACCGGA AGCTGGAGGA GCAGCTGGAA
    AAAATAAGCC ACCAAGACCA CAGTGACAAG AACCGTCTGC TGGAGCTGGA GACCAGGCTA
    AGGGAGGTCA GTCTAGAACA TGAAGAGCAG AAACTGGAGC TCAAACGCCA GCTCACAGAG
    CTGCAGCTCT CCCTGCAGGA GCGTGAATCC CAGCTGACAG CCCTGCAGGC AGCACGGGCG
    GCCCTGGAGA GCCAGCTCCG CCAGGCCAAG ACGGAGCTGG AAGAGACGAC AGCAGAAGCA
    GAAGAAGAGA TCCAGGCGCT TACGGCACAT AGAGATGAAA TCCAGCGCAA ATTCGATGCC
    CTTCGTAACA GCTGTACTGT GATCACGGAC TTGGAGGAGC AGCTAAACCA GCTGACGGAG
    GACAATGCCG AGCTCAACAA CCAAAACTTC TACTTGTCTA AACAACTCGA TGAGGCCTCT
    GGTGCCAACG ACGAGATTGT GCAGCTGCGA AGTGAAGTGG ACCATCTCCG CCGCGAGATC
    ACTGAGAGGG AGATGCAGCT CACTAGCCAG AAGCAAACGA TGGAGGCGCT GAAGACCACG
    TGCACAATGC TGGAGGAGCA GGTCATGGAC CTGGAGGCCC TGAACGACGA GCTGCTGGAG
    AAGGAGCGGC AGTGGGAGGC CTGGAGGAGT GTCCTTGGTG ACGAGAAGTC CCAGTTTGAG
    TGTCGGGTTC GAGAGTTGCA GAGGATGCTG GACACCGAAA AGCAGAGCAG GGCGAGGGCC
    GACCAGAGGA TCACTGAGTC GCGCCAGGTG GTCGAGCTGG CAGTAAAGGA GCACAAGGCC
    GAGATCCTGG CCTTGCAGCA GGCTCTCAAG GAGCAGAAGC TGAAGGCCGA GAGCCTCTCC
    GACAAGCTCA ATGATCTGGA GAAGAAGCAC GCCATGCTTG AAATGAATGC CCGAAGTTTA
    CAACAGAAGC TGGAGACAGA ACGAGAGCTC AAACAAAGGC TTCTGGAAGA GCAAGCCAAG
    TTACAGCAGC AGATGGACCT GCAGAAGAAT CATATTTTCC GTCTGACTCA AGGGCTGCAA
    GAAGCTCTGG ATCGGGCTGA TCTGCTGAAG ACAGAAAGGA GTGACCTGGA ATATCAGCTG
    GAAAACATTC AGGTTCTCTA TTCTCATGAG AAGGTGAAGA TGGAAGGCAC TATTTCCCAG
    CAAACCAAAC TCATCGATTT TCTGCAAGCC AAAATGGACC AGCCTGCCAA AAAGAAAAAG
    GGTTTATTTA GTCGACGGAA AGAGGACCCT GCTTTGCCCA CACAGGTTCC TCTGCAGTAC
    AATGAGCTGA AGGTGGCTCT GGAGAAGGAG AAAGCTCGCT GCGCGGAGCT CGAGGAAGCC
    CTGCAGAAGA CCCGCATCGA GCTCCGCTCC GCCCGGGAGG AAGCTGCCCA CCGGAAAGCT
    GCAGACCACC CACACCCATC TACGCCGGCT ACCGCCAGGC AGCAGATCGC CATGTCCGCC
    ATTGTGCGGT CACCCGAGCA CCAGCCCAGC GCCCTGAGCC TGCTCGCCCC GCCGTCCAGC
    CGCAGGAAGG AGTCTTCCAC TCCAGAGGAA TTCAGCCGGC GTCTTAAGGA GCGCATGCAC
    CACAACATCC CTCACCGGTT CAACGTGGGA CTGAACATGC GAGCCACGAA GTGCGCCGTG
    TGTCTGGATA CCGTGCACTT CGGGCGCCAG GCATCCAAGT GTCTCGAATG TCAGGTCATG
    TGCCACCCCA AGTGCTCCAC ATGCTTGCCA GCCACCTGTG GCCTGCCAGC CGAATACGCC
    ACACACTTCA CCGAGGCCTT CTGCCGCGAC AAAATGAACT CCCCGGGTCT GCAGTCCAAG
    GAGCCCAGCA GCAGCCTGCA CCTGGAAGGG TGGATGAAGG TGCCCAGGAA TAACAAGCGA
    GGACAGCAAG GCTGGGACAG GAAGTACATC GTTCTGGAGG GCTCCAAAGT CCTCATTTAT
    GACAACGAAG CCAGAGAAGC TGGACAGAGG CCGGTGGAAG AATTTGAGCT GTGCCTTCCC
    GACGGGGACG TGTCTATTCA TGGCGCCGTT GGTGCTTCTG AACTTGCAAA CACAGCCAAA
    GCAGATGTCC CCTATGTCCT GAAGATGGAG TCTCACCCCC ATACCACCTG CTGGCCTGGG
    AGGACCCTCT ACTTGCTGGC TCCCAGCTTT CCCGACAAGC AGCGCTGGGT CACCGCCTTA
    GAGTCAGTTG TGGCAGGTGG GAGAGTGTCT AGGGAAAAGG CGGAAGCCGA CGCTGGCCGT
    GACTGTACTT CCTACGAGCC TCTGCCCGCG TGGGCTCAGA AATTGCTCGG AAACTCCCTG
    TTGAAACTGG AAGGTGATGA CCGTCTGGAC ATGAACTGCA CGCTGCCCTT CAGCGACCAG
    GTGGTGCTGG TGGGCACCGA GGAAGGGCTG TATGCGCTGA ATGTCTTGAA GAACTCCCTC
    ACCCACGTCC CAGGAATCGG AGCAGTCTTC CAGATTTACA TCATCAAGGA CCTGGAGAAG
    CTCCTCATGA TCGCAGGAGA GGAGCGGGCC CTGTGTCTCG TGGATGTGAA GAAGGTGAAG
    CAGTCCCTCG CGCAGTCTCA CCTCCCCGCC CAGCCAGACA TCTCGCCCAA CATCTTTGAA
    GCGGTCAAGG GCTGCCACTT GTTTGCTGCT GGCAAGATTG AGAGCGGGCT CTGCATCTGT
    GCAGCCATGC CCAGCAAAGT TGTCATTCTC CGCTACAATG AAAACCTCAG CAAGTACTGC
    ATTCGCAAAG AGATCGAGAC CTCGGAGCCC TGCAGCTGTA TCCACTTCAC CAACTACAGT
    ATCCTCATCG GAACCAATAA ATTCTACGAA ATCGACATGA AGCAGTACAC GCTAGAGGAA
    TTCCTGGATA AGAACGACCA TTCCTTGGCG CCTGCCGTGT TTGCCTCCTC TTCCAACAGC
    TTCCCCGTGT CCATCATGCA GGTGAACGGC GCGGGGCAGC GGGAGGAGTT CCTGCTCTGT
    TTCCACGAAT TCGGGGTGTT CGTGGATTCT TATGGAAGAC GCAGCCGCAC AGATGATCTC
    AAGTGGAGTC GCTTACCTCT GGCCTTTGCC TACAGAGAAC CCTATCTGTT TGTGACTCAC
    TTCAACTCAC TCGAAGTAAT TGAGATCCAG GCCCGCTCCT CTCTGGGGAC CCCCGCCCGA
    GCATATTTGG AAATCCCCAA CCCACGCTAC CTGGGCCCTG CAATCTCCTC AGGAGCCATC
    TACCTGGCGT CCTCATATCA GGATAAGTTA AGGGTCATCT GCTGCAAAGG AAACCTGGTG
    AAGGAGACCG GCACCGACCA CCACCGTGGC CCATCCACCT CCCGCAGCCC CAACAAGCGA
    GGCCCGCCGA CGTACAACGA GCACATCACC AAGCGCGTGG CCTCAAGCCC GGCGCCTCCC
    GAAGGCCCCA GCCACCCTCG AGAGCCAAGC ACACCCCACC GCTACCGCGA GGGGCGGACC
    GAGCTGCGCA GGGACAAGTC TCCGGGCCGC CCCCTGGAGC GCGAGAAGTC CCCCGGCCGG
    ATGCTGAGCA CGCGGAGGGA GCGCTCCCCC GGGCGGCTGT TTGAGGACAG CAGCAGGGGC
    CGGCTGCCCG TGGGGGCCGT GAGGACCCCG CTGTCCCAGG TCAACAAGGT CTGGGACCAG
    TCTTCAGTAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa224622
    Clone ID Related Accession (Same CDS sequence) XM_024977733.1 , XM_024977734.1
    Accession Version XM_024977734.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6252bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product citron Rho-interacting kinase isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGTTGAAGT TCAAGTATGG AGCACGGAAT CTACTGGACG CTGGTACTGT TGAATCCATT 
    GCCAGCCGGG CCTCCAGGCT GAATCTTTTC TTCCAGGGGA AACCACCCTC CATGACTCAA
    CAGCAGATGT CTCTTCTTTC CCGAGAAGCG ATATTAGATG CCCTCTTTGT TCTCTTTAAA
    GAATGCAGTC AGCCAGCTCT GATGAAGATT AAGCACGTGA GCAACTTTGT CCAAAAGTAT
    TCCGATATCA TAGCTGAGTT GCAAGAGCTC CAGCCTTCAG TGAAGGACTT TGAAGTGAAA
    AGTGTCGTAG GCTGCGGTCA CTTTGCTGAA GTACAGGTGG TCAGAGAGAA AGCGACTGGG
    GATGTCTATG CCATGAAAGT CATGAAGAAG AAGGCCTTGT TGGCCCAGGA GCAGGTCTCA
    TATTTTGAGG AAGAACAGAA CATATTATCT CAGAGTACAA GTCCTTGGAT CCCCCAGTTG
    CAGTATGCCT TTCAGGACAA AAATAACCTT TATCTGGTCA TGGAATATCA GCCTGGAGGG
    GATTTGCTGT CACTTTTGAA TAGATATGAG GACCAATTAG ATGAAAATAT GATTCAGTTT
    TACCTAGCTG AACTGATTTT GGCTGTCCAC AGCGTTCATC AGATGGGCTA TGTACATCGA
    GACATCAAGC CCGAGAACAT CCTCATTGAC CGAACGGGAC ACATCAAGCT GGTGGATTTT
    GGATCAGCTG CTAAAATGAA CTCAAATAAG ATGGTAAATG CCAAACTCCC GGTTGGTACT
    CCAGATTACA TGGCCCCTGA AGTGTTGACT GTGATGAATG GGGATGGAAA AGGTGCCTAT
    AGCCTAGACT GTGATTGGTG GTCAGTGGGA GTTATTGCTT ATGAGATGGT TTATGGAAGA
    TGCCCATTCA CTGAAGGAAC CTCAGCCAAA ACCTTCAATA ACATCATGAA TTTCCAGCGG
    TTCTTGAAGT TTCCAGATGA CCCCAAAGTT AGCAGTGAAT TGCTTGATCT AATCCAGAGT
    TTGTTGTGTG GCCAGAAAGA GAGACTGAAG TTTGAGGGCC TTTGCTGTCA TCCTTTCTTC
    TCCAAAATTG ACTGGATTAA CATTCGTAAC TCTCCTCCCC CCTTCGTTCC CACCCTCAAG
    TCTGATGATG ACACCTCCAA TTTTGATGAA CCAGAGAAGA ATTCGTGGGT TTCATCCTCT
    CCGTGCCAGC TGAACCCCTC AGGTTTCTCG GGCGAAGAAC TGCCGTTTGT GGGGTTTTCG
    TATAGCAAGG CACTGGGAAT TCTTGGTAGA TCTGAGCCTG TTGTATCAAG TCTGGACTCC
    CCTGCCAAGA CTAGCTCCAT GGAAAAGAAA CTCCTTATCA AAAGCAAAGA GCTGCAGGAC
    TCCCAGGACA AGTGTCACAA GATGGAGCAG GAAATGACCC GGTTACATCG GAGAGTGTCA
    GAGGTGGAGG CTGTGCTTAG TCAGAAGGAG GTGGAGCTGA AGGCCTCTGA GACTCAGAGA
    TCCCTCCTGG AGCAGGACCT TGCTACCTAC ATCACAGAAT GCAGTAGCTT AAAGCGAAGT
    TTGGAGCAAG CACGGATGGA GGTGTCCCAA GAGGACGACA AAGCACTGCA GCTCCTCCAT
    GATATCAGAG AGCAGAGCCG GAAGCTCCAA GAAATCAAAG AGCAGGAGTA CCAGGCTCAA
    GTGGAAGAAA TGAGGTTGAT GATGAATCAG TTGGAAGAGG ACCTGGTTTC GGCGAGAAGG
    CGGAGTGACC TCTATGAATC GGAGCTCAGA GAGTCTCGGC TGGCTGCTGA AGAGTTTAAG
    CGGAAAGCAA CAGAATGTCA GCATAAACTG ATGAAGGCTA AAGATCAAGG GAAGCCTGAA
    GTGGGAGAAT ATGCCAAACT GGAGAAGATC AATGCTGAGC AGCAGCTCAA AATTCAGGAG
    CTCCAAGAGA AGCTAGAAAA GGCTGTGAAA GCTAGCACAG AGGCCACCGA GCTGCTGCAG
    AACATCCGTC AGGCGAAGGA GCGGGCCGAG CGTGAGCTGG AGAAGCTGCA CAACCGTGAG
    GACTCCTCCG AGGGCATCAA GAAGAAGCTG GTGGAGGCCG AGGAACGCCG CCACTCTCTG
    GAGAACAAGG TAAAGAGGCT AGAGACCATG GAGCGTAGAG AAAACAGACT GAAGGATGAC
    ATCCAGACAA AGTCCCAACA GATCCAGCAG ATGGCTGATA AAATTCTGGA GCTGGAGGAG
    AAGCACAGAG AAGCCCAGGT CTCAGCGCAG CATCTGGAGG TGCACCTGAA ACAGAAAGAG
    CAGCACTATG AAGAAAAAAT TAAAGTGTTG GACAATCAGA TAAAGAAAGA CCTAGCAGAC
    AAGGAGACAC TGGAGAACCT GATGCAAAGA CACGAAGAGG AGGCCCATGA GAAAGGCAAA
    ATTCTCAGCG AGCAGAAGGC GATGATCAAT GCTATGGATT CCAAGATCAG ATCCCTGGAA
    CAAAGGATAG TGGAATTGTC GGAAGCCAAT AAACTTGCGG CCAACAGCAG TCTCTTTACC
    CAGAGGAACA TGAAGGCCCA GGAAGAGATG ATTTCAGAAC TCAGGCAACA GAAGTTTTAC
    CTGGAGACGC AAGCTGGGAA ATTGGAGGCC CAGAACCGGA AGCTGGAGGA GCAGCTGGAA
    AAAATAAGCC ACCAAGACCA CAGTGACAAG AACCGTCTGC TGGAGCTGGA GACCAGGCTA
    AGGGAGGTCA GTCTAGAACA TGAAGAGCAG AAACTGGAGC TCAAACGCCA GCTCACAGAG
    CTGCAGCTCT CCCTGCAGGA GCGTGAATCC CAGCTGACAG CCCTGCAGGC AGCACGGGCG
    GCCCTGGAGA GCCAGCTCCG CCAGGCCAAG ACGGAGCTGG AAGAGACGAC AGCAGAAGCA
    GAAGAAGAGA TCCAGGCGCT TACGGCACAT AGAGATGAAA TCCAGCGCAA ATTCGATGCC
    CTTCGTAACA GCTGTACTGT GATCACGGAC TTGGAGGAGC AGCTAAACCA GCTGACGGAG
    GACAATGCCG AGCTCAACAA CCAAAACTTC TACTTGTCTA AACAACTCGA TGAGGCCTCT
    GGTGCCAACG ACGAGATTGT GCAGCTGCGA AGTGAAGTGG ACCATCTCCG CCGCGAGATC
    ACTGAGAGGG AGATGCAGCT CACTAGCCAG AAGCAAACGA TGGAGGCGCT GAAGACCACG
    TGCACAATGC TGGAGGAGCA GGTCATGGAC CTGGAGGCCC TGAACGACGA GCTGCTGGAG
    AAGGAGCGGC AGTGGGAGGC CTGGAGGAGT GTCCTTGGTG ACGAGAAGTC CCAGTTTGAG
    TGTCGGGTTC GAGAGTTGCA GAGGATGCTG GACACCGAAA AGCAGAGCAG GGCGAGGGCC
    GACCAGAGGA TCACTGAGTC GCGCCAGGTG GTCGAGCTGG CAGTAAAGGA GCACAAGGCC
    GAGATCCTGG CCTTGCAGCA GGCTCTCAAG GAGCAGAAGC TGAAGGCCGA GAGCCTCTCC
    GACAAGCTCA ATGATCTGGA GAAGAAGCAC GCCATGCTTG AAATGAATGC CCGAAGTTTA
    CAACAGAAGC TGGAGACAGA ACGAGAGCTC AAACAAAGGC TTCTGGAAGA GCAAGCCAAG
    TTACAGCAGC AGATGGACCT GCAGAAGAAT CATATTTTCC GTCTGACTCA AGGGCTGCAA
    GAAGCTCTGG ATCGGGCTGA TCTGCTGAAG ACAGAAAGGA GTGACCTGGA ATATCAGCTG
    GAAAACATTC AGGTTCTCTA TTCTCATGAG AAGGTGAAGA TGGAAGGCAC TATTTCCCAG
    CAAACCAAAC TCATCGATTT TCTGCAAGCC AAAATGGACC AGCCTGCCAA AAAGAAAAAG
    GGTTTATTTA GTCGACGGAA AGAGGACCCT GCTTTGCCCA CACAGGTTCC TCTGCAGTAC
    AATGAGCTGA AGGTGGCTCT GGAGAAGGAG AAAGCTCGCT GCGCGGAGCT CGAGGAAGCC
    CTGCAGAAGA CCCGCATCGA GCTCCGCTCC GCCCGGGAGG AAGCTGCCCA CCGGAAAGCT
    GCAGACCACC CACACCCATC TACGCCGGCT ACCGCCAGGC AGCAGATCGC CATGTCCGCC
    ATTGTGCGGT CACCCGAGCA CCAGCCCAGC GCCCTGAGCC TGCTCGCCCC GCCGTCCAGC
    CGCAGGAAGG AGTCTTCCAC TCCAGAGGAA TTCAGCCGGC GTCTTAAGGA GCGCATGCAC
    CACAACATCC CTCACCGGTT CAACGTGGGA CTGAACATGC GAGCCACGAA GTGCGCCGTG
    TGTCTGGATA CCGTGCACTT CGGGCGCCAG GCATCCAAGT GTCTCGAATG TCAGGTCATG
    TGCCACCCCA AGTGCTCCAC ATGCTTGCCA GCCACCTGTG GCCTGCCAGC CGAATACGCC
    ACACACTTCA CCGAGGCCTT CTGCCGCGAC AAAATGAACT CCCCGGGTCT GCAGTCCAAG
    GAGCCCAGCA GCAGCCTGCA CCTGGAAGGG TGGATGAAGG TGCCCAGGAA TAACAAGCGA
    GGACAGCAAG GCTGGGACAG GAAGTACATC GTTCTGGAGG GCTCCAAAGT CCTCATTTAT
    GACAACGAAG CCAGAGAAGC TGGACAGAGG CCGGTGGAAG AATTTGAGCT GTGCCTTCCC
    GACGGGGACG TGTCTATTCA TGGCGCCGTT GGTGCTTCTG AACTTGCAAA CACAGCCAAA
    GCAGATGTCC CCTATGTCCT GAAGATGGAG TCTCACCCCC ATACCACCTG CTGGCCTGGG
    AGGACCCTCT ACTTGCTGGC TCCCAGCTTT CCCGACAAGC AGCGCTGGGT CACCGCCTTA
    GAGTCAGTTG TGGCAGGTGG GAGAGTGTCT AGGGAAAAGG CGGAAGCCGA CGCTGGCCGT
    GACTGTACTT CCTACGAGCC TCTGCCCGCG TGGGCTCAGA AATTGCTCGG AAACTCCCTG
    TTGAAACTGG AAGGTGATGA CCGTCTGGAC ATGAACTGCA CGCTGCCCTT CAGCGACCAG
    GTGGTGCTGG TGGGCACCGA GGAAGGGCTG TATGCGCTGA ATGTCTTGAA GAACTCCCTC
    ACCCACGTCC CAGGAATCGG AGCAGTCTTC CAGATTTACA TCATCAAGGA CCTGGAGAAG
    CTCCTCATGA TCGCAGGAGA GGAGCGGGCC CTGTGTCTCG TGGATGTGAA GAAGGTGAAG
    CAGTCCCTCG CGCAGTCTCA CCTCCCCGCC CAGCCAGACA TCTCGCCCAA CATCTTTGAA
    GCGGTCAAGG GCTGCCACTT GTTTGCTGCT GGCAAGATTG AGAGCGGGCT CTGCATCTGT
    GCAGCCATGC CCAGCAAAGT TGTCATTCTC CGCTACAATG AAAACCTCAG CAAGTACTGC
    ATTCGCAAAG AGATCGAGAC CTCGGAGCCC TGCAGCTGTA TCCACTTCAC CAACTACAGT
    ATCCTCATCG GAACCAATAA ATTCTACGAA ATCGACATGA AGCAGTACAC GCTAGAGGAA
    TTCCTGGATA AGAACGACCA TTCCTTGGCG CCTGCCGTGT TTGCCTCCTC TTCCAACAGC
    TTCCCCGTGT CCATCATGCA GGTGAACGGC GCGGGGCAGC GGGAGGAGTT CCTGCTCTGT
    TTCCACGAAT TCGGGGTGTT CGTGGATTCT TATGGAAGAC GCAGCCGCAC AGATGATCTC
    AAGTGGAGTC GCTTACCTCT GGCCTTTGCC TACAGAGAAC CCTATCTGTT TGTGACTCAC
    TTCAACTCAC TCGAAGTAAT TGAGATCCAG GCCCGCTCCT CTCTGGGGAC CCCCGCCCGA
    GCATATTTGG AAATCCCCAA CCCACGCTAC CTGGGCCCTG CAATCTCCTC AGGAGCCATC
    TACCTGGCGT CCTCATATCA GGATAAGTTA AGGGTCATCT GCTGCAAAGG AAACCTGGTG
    AAGGAGACCG GCACCGACCA CCACCGTGGC CCATCCACCT CCCGCAGCCC CAACAAGCGA
    GGCCCGCCGA CGTACAACGA GCACATCACC AAGCGCGTGG CCTCAAGCCC GGCGCCTCCC
    GAAGGCCCCA GCCACCCTCG AGAGCCAAGC ACACCCCACC GCTACCGCGA GGGGCGGACC
    GAGCTGCGCA GGGACAAGTC TCCGGGCCGC CCCCTGGAGC GCGAGAAGTC CCCCGGCCGG
    ATGCTGAGCA CGCGGAGGGA GCGCTCCCCC GGGCGGCTGT TTGAGGACAG CAGCAGGGGC
    CGGCTGCCCG TGGGGGCCGT GAGGACCCCG CTGTCCCAGG TCAACAAGGT CTGGGACCAG
    TCTTCAGTAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024833502.1
    CDS52..6303
    Translation

    Target ORF information:

    RefSeq Version XM_024977734.1
    Organism Bos taurus(cattle)
    Definition Bos taurus citron rho-interacting serine/threonine kinase (CIT), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024977734.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGTTGAAGT TCAAGTATGG AGCACGGAAT CTACTGGACG CTGGTACTGT TGAATCCATT 
    GCCAGCCGGG CCTCCAGGCT GAATCTTTTC TTCCAGGGGA AACCACCCTC CATGACTCAA
    CAGCAGATGT CTCTTCTTTC CCGAGAAGCG ATATTAGATG CCCTCTTTGT TCTCTTTAAA
    GAATGCAGTC AGCCAGCTCT GATGAAGATT AAGCACGTGA GCAACTTTGT CCAAAAGTAT
    TCCGATATCA TAGCTGAGTT GCAAGAGCTC CAGCCTTCAG TGAAGGACTT TGAAGTGAAA
    AGTGTCGTAG GCTGCGGTCA CTTTGCTGAA GTACAGGTGG TCAGAGAGAA AGCGACTGGG
    GATGTCTATG CCATGAAAGT CATGAAGAAG AAGGCCTTGT TGGCCCAGGA GCAGGTCTCA
    TATTTTGAGG AAGAACAGAA CATATTATCT CAGAGTACAA GTCCTTGGAT CCCCCAGTTG
    CAGTATGCCT TTCAGGACAA AAATAACCTT TATCTGGTCA TGGAATATCA GCCTGGAGGG
    GATTTGCTGT CACTTTTGAA TAGATATGAG GACCAATTAG ATGAAAATAT GATTCAGTTT
    TACCTAGCTG AACTGATTTT GGCTGTCCAC AGCGTTCATC AGATGGGCTA TGTACATCGA
    GACATCAAGC CCGAGAACAT CCTCATTGAC CGAACGGGAC ACATCAAGCT GGTGGATTTT
    GGATCAGCTG CTAAAATGAA CTCAAATAAG ATGGTAAATG CCAAACTCCC GGTTGGTACT
    CCAGATTACA TGGCCCCTGA AGTGTTGACT GTGATGAATG GGGATGGAAA AGGTGCCTAT
    AGCCTAGACT GTGATTGGTG GTCAGTGGGA GTTATTGCTT ATGAGATGGT TTATGGAAGA
    TGCCCATTCA CTGAAGGAAC CTCAGCCAAA ACCTTCAATA ACATCATGAA TTTCCAGCGG
    TTCTTGAAGT TTCCAGATGA CCCCAAAGTT AGCAGTGAAT TGCTTGATCT AATCCAGAGT
    TTGTTGTGTG GCCAGAAAGA GAGACTGAAG TTTGAGGGCC TTTGCTGTCA TCCTTTCTTC
    TCCAAAATTG ACTGGATTAA CATTCGTAAC TCTCCTCCCC CCTTCGTTCC CACCCTCAAG
    TCTGATGATG ACACCTCCAA TTTTGATGAA CCAGAGAAGA ATTCGTGGGT TTCATCCTCT
    CCGTGCCAGC TGAACCCCTC AGGTTTCTCG GGCGAAGAAC TGCCGTTTGT GGGGTTTTCG
    TATAGCAAGG CACTGGGAAT TCTTGGTAGA TCTGAGCCTG TTGTATCAAG TCTGGACTCC
    CCTGCCAAGA CTAGCTCCAT GGAAAAGAAA CTCCTTATCA AAAGCAAAGA GCTGCAGGAC
    TCCCAGGACA AGTGTCACAA GATGGAGCAG GAAATGACCC GGTTACATCG GAGAGTGTCA
    GAGGTGGAGG CTGTGCTTAG TCAGAAGGAG GTGGAGCTGA AGGCCTCTGA GACTCAGAGA
    TCCCTCCTGG AGCAGGACCT TGCTACCTAC ATCACAGAAT GCAGTAGCTT AAAGCGAAGT
    TTGGAGCAAG CACGGATGGA GGTGTCCCAA GAGGACGACA AAGCACTGCA GCTCCTCCAT
    GATATCAGAG AGCAGAGCCG GAAGCTCCAA GAAATCAAAG AGCAGGAGTA CCAGGCTCAA
    GTGGAAGAAA TGAGGTTGAT GATGAATCAG TTGGAAGAGG ACCTGGTTTC GGCGAGAAGG
    CGGAGTGACC TCTATGAATC GGAGCTCAGA GAGTCTCGGC TGGCTGCTGA AGAGTTTAAG
    CGGAAAGCAA CAGAATGTCA GCATAAACTG ATGAAGGCTA AAGATCAAGG GAAGCCTGAA
    GTGGGAGAAT ATGCCAAACT GGAGAAGATC AATGCTGAGC AGCAGCTCAA AATTCAGGAG
    CTCCAAGAGA AGCTAGAAAA GGCTGTGAAA GCTAGCACAG AGGCCACCGA GCTGCTGCAG
    AACATCCGTC AGGCGAAGGA GCGGGCCGAG CGTGAGCTGG AGAAGCTGCA CAACCGTGAG
    GACTCCTCCG AGGGCATCAA GAAGAAGCTG GTGGAGGCCG AGGAACGCCG CCACTCTCTG
    GAGAACAAGG TAAAGAGGCT AGAGACCATG GAGCGTAGAG AAAACAGACT GAAGGATGAC
    ATCCAGACAA AGTCCCAACA GATCCAGCAG ATGGCTGATA AAATTCTGGA GCTGGAGGAG
    AAGCACAGAG AAGCCCAGGT CTCAGCGCAG CATCTGGAGG TGCACCTGAA ACAGAAAGAG
    CAGCACTATG AAGAAAAAAT TAAAGTGTTG GACAATCAGA TAAAGAAAGA CCTAGCAGAC
    AAGGAGACAC TGGAGAACCT GATGCAAAGA CACGAAGAGG AGGCCCATGA GAAAGGCAAA
    ATTCTCAGCG AGCAGAAGGC GATGATCAAT GCTATGGATT CCAAGATCAG ATCCCTGGAA
    CAAAGGATAG TGGAATTGTC GGAAGCCAAT AAACTTGCGG CCAACAGCAG TCTCTTTACC
    CAGAGGAACA TGAAGGCCCA GGAAGAGATG ATTTCAGAAC TCAGGCAACA GAAGTTTTAC
    CTGGAGACGC AAGCTGGGAA ATTGGAGGCC CAGAACCGGA AGCTGGAGGA GCAGCTGGAA
    AAAATAAGCC ACCAAGACCA CAGTGACAAG AACCGTCTGC TGGAGCTGGA GACCAGGCTA
    AGGGAGGTCA GTCTAGAACA TGAAGAGCAG AAACTGGAGC TCAAACGCCA GCTCACAGAG
    CTGCAGCTCT CCCTGCAGGA GCGTGAATCC CAGCTGACAG CCCTGCAGGC AGCACGGGCG
    GCCCTGGAGA GCCAGCTCCG CCAGGCCAAG ACGGAGCTGG AAGAGACGAC AGCAGAAGCA
    GAAGAAGAGA TCCAGGCGCT TACGGCACAT AGAGATGAAA TCCAGCGCAA ATTCGATGCC
    CTTCGTAACA GCTGTACTGT GATCACGGAC TTGGAGGAGC AGCTAAACCA GCTGACGGAG
    GACAATGCCG AGCTCAACAA CCAAAACTTC TACTTGTCTA AACAACTCGA TGAGGCCTCT
    GGTGCCAACG ACGAGATTGT GCAGCTGCGA AGTGAAGTGG ACCATCTCCG CCGCGAGATC
    ACTGAGAGGG AGATGCAGCT CACTAGCCAG AAGCAAACGA TGGAGGCGCT GAAGACCACG
    TGCACAATGC TGGAGGAGCA GGTCATGGAC CTGGAGGCCC TGAACGACGA GCTGCTGGAG
    AAGGAGCGGC AGTGGGAGGC CTGGAGGAGT GTCCTTGGTG ACGAGAAGTC CCAGTTTGAG
    TGTCGGGTTC GAGAGTTGCA GAGGATGCTG GACACCGAAA AGCAGAGCAG GGCGAGGGCC
    GACCAGAGGA TCACTGAGTC GCGCCAGGTG GTCGAGCTGG CAGTAAAGGA GCACAAGGCC
    GAGATCCTGG CCTTGCAGCA GGCTCTCAAG GAGCAGAAGC TGAAGGCCGA GAGCCTCTCC
    GACAAGCTCA ATGATCTGGA GAAGAAGCAC GCCATGCTTG AAATGAATGC CCGAAGTTTA
    CAACAGAAGC TGGAGACAGA ACGAGAGCTC AAACAAAGGC TTCTGGAAGA GCAAGCCAAG
    TTACAGCAGC AGATGGACCT GCAGAAGAAT CATATTTTCC GTCTGACTCA AGGGCTGCAA
    GAAGCTCTGG ATCGGGCTGA TCTGCTGAAG ACAGAAAGGA GTGACCTGGA ATATCAGCTG
    GAAAACATTC AGGTTCTCTA TTCTCATGAG AAGGTGAAGA TGGAAGGCAC TATTTCCCAG
    CAAACCAAAC TCATCGATTT TCTGCAAGCC AAAATGGACC AGCCTGCCAA AAAGAAAAAG
    GGTTTATTTA GTCGACGGAA AGAGGACCCT GCTTTGCCCA CACAGGTTCC TCTGCAGTAC
    AATGAGCTGA AGGTGGCTCT GGAGAAGGAG AAAGCTCGCT GCGCGGAGCT CGAGGAAGCC
    CTGCAGAAGA CCCGCATCGA GCTCCGCTCC GCCCGGGAGG AAGCTGCCCA CCGGAAAGCT
    GCAGACCACC CACACCCATC TACGCCGGCT ACCGCCAGGC AGCAGATCGC CATGTCCGCC
    ATTGTGCGGT CACCCGAGCA CCAGCCCAGC GCCCTGAGCC TGCTCGCCCC GCCGTCCAGC
    CGCAGGAAGG AGTCTTCCAC TCCAGAGGAA TTCAGCCGGC GTCTTAAGGA GCGCATGCAC
    CACAACATCC CTCACCGGTT CAACGTGGGA CTGAACATGC GAGCCACGAA GTGCGCCGTG
    TGTCTGGATA CCGTGCACTT CGGGCGCCAG GCATCCAAGT GTCTCGAATG TCAGGTCATG
    TGCCACCCCA AGTGCTCCAC ATGCTTGCCA GCCACCTGTG GCCTGCCAGC CGAATACGCC
    ACACACTTCA CCGAGGCCTT CTGCCGCGAC AAAATGAACT CCCCGGGTCT GCAGTCCAAG
    GAGCCCAGCA GCAGCCTGCA CCTGGAAGGG TGGATGAAGG TGCCCAGGAA TAACAAGCGA
    GGACAGCAAG GCTGGGACAG GAAGTACATC GTTCTGGAGG GCTCCAAAGT CCTCATTTAT
    GACAACGAAG CCAGAGAAGC TGGACAGAGG CCGGTGGAAG AATTTGAGCT GTGCCTTCCC
    GACGGGGACG TGTCTATTCA TGGCGCCGTT GGTGCTTCTG AACTTGCAAA CACAGCCAAA
    GCAGATGTCC CCTATGTCCT GAAGATGGAG TCTCACCCCC ATACCACCTG CTGGCCTGGG
    AGGACCCTCT ACTTGCTGGC TCCCAGCTTT CCCGACAAGC AGCGCTGGGT CACCGCCTTA
    GAGTCAGTTG TGGCAGGTGG GAGAGTGTCT AGGGAAAAGG CGGAAGCCGA CGCTGGCCGT
    GACTGTACTT CCTACGAGCC TCTGCCCGCG TGGGCTCAGA AATTGCTCGG AAACTCCCTG
    TTGAAACTGG AAGGTGATGA CCGTCTGGAC ATGAACTGCA CGCTGCCCTT CAGCGACCAG
    GTGGTGCTGG TGGGCACCGA GGAAGGGCTG TATGCGCTGA ATGTCTTGAA GAACTCCCTC
    ACCCACGTCC CAGGAATCGG AGCAGTCTTC CAGATTTACA TCATCAAGGA CCTGGAGAAG
    CTCCTCATGA TCGCAGGAGA GGAGCGGGCC CTGTGTCTCG TGGATGTGAA GAAGGTGAAG
    CAGTCCCTCG CGCAGTCTCA CCTCCCCGCC CAGCCAGACA TCTCGCCCAA CATCTTTGAA
    GCGGTCAAGG GCTGCCACTT GTTTGCTGCT GGCAAGATTG AGAGCGGGCT CTGCATCTGT
    GCAGCCATGC CCAGCAAAGT TGTCATTCTC CGCTACAATG AAAACCTCAG CAAGTACTGC
    ATTCGCAAAG AGATCGAGAC CTCGGAGCCC TGCAGCTGTA TCCACTTCAC CAACTACAGT
    ATCCTCATCG GAACCAATAA ATTCTACGAA ATCGACATGA AGCAGTACAC GCTAGAGGAA
    TTCCTGGATA AGAACGACCA TTCCTTGGCG CCTGCCGTGT TTGCCTCCTC TTCCAACAGC
    TTCCCCGTGT CCATCATGCA GGTGAACGGC GCGGGGCAGC GGGAGGAGTT CCTGCTCTGT
    TTCCACGAAT TCGGGGTGTT CGTGGATTCT TATGGAAGAC GCAGCCGCAC AGATGATCTC
    AAGTGGAGTC GCTTACCTCT GGCCTTTGCC TACAGAGAAC CCTATCTGTT TGTGACTCAC
    TTCAACTCAC TCGAAGTAAT TGAGATCCAG GCCCGCTCCT CTCTGGGGAC CCCCGCCCGA
    GCATATTTGG AAATCCCCAA CCCACGCTAC CTGGGCCCTG CAATCTCCTC AGGAGCCATC
    TACCTGGCGT CCTCATATCA GGATAAGTTA AGGGTCATCT GCTGCAAAGG AAACCTGGTG
    AAGGAGACCG GCACCGACCA CCACCGTGGC CCATCCACCT CCCGCAGCCC CAACAAGCGA
    GGCCCGCCGA CGTACAACGA GCACATCACC AAGCGCGTGG CCTCAAGCCC GGCGCCTCCC
    GAAGGCCCCA GCCACCCTCG AGAGCCAAGC ACACCCCACC GCTACCGCGA GGGGCGGACC
    GAGCTGCGCA GGGACAAGTC TCCGGGCCGC CCCCTGGAGC GCGAGAAGTC CCCCGGCCGG
    ATGCTGAGCA CGCGGAGGGA GCGCTCCCCC GGGCGGCTGT TTGAGGACAG CAGCAGGGGC
    CGGCTGCCCG TGGGGGCCGT GAGGACCCCG CTGTCCCAGG TCAACAAGGT CTGGGACCAG
    TCTTCAGTAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa224623
    Clone ID Related Accession (Same CDS sequence) XM_024977735.1
    Accession Version XM_024977735.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6207bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product citron Rho-interacting kinase isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    ATGTTGAAGT TCAAGTATGG AGCACGGAAT CTACTGGACG CTGGTACTGT TGAATCCATT 
    GCCAGCCGGG CCTCCAGGCT GAATCTTTTC TTCCAGGGGA AACCACCCTC CATGACTCAA
    CAGCAGATGT CTCTTCTTTC CCGAGAAGCG ATATTAGATG CCCTCTTTGT TCTCTTTAAA
    GAATGCAGTC AGCCAGCTCT GATGAAGATT AAGCACGTGA GCAACTTTGT CCAAAAGTAT
    TCCGATATCA TAGCTGAGTT GCAAGAGCTC CAGCCTTCAG TGAAGGACTT TGAAGTGAAA
    AGTGTCGTAG GCTGCGGTCA CTTTGCTGAA GTACAGGTGG TCAGAGAGAA AGCGACTGGG
    GATGTCTATG CCATGAAAGT CATGAAGAAG AAGGCCTTGT TGGCCCAGGA GCAGGTCTCA
    TATTTTGAGG AAGAACAGAA CATATTATCT CAGAGTACAA GTCCTTGGAT CCCCCAGTTG
    CAGTATGCCT TTCAGGACAA AAATAACCTT TATCTGGTCA TGGAATATCA GCCTGGAGGG
    GATTTGCTGT CACTTTTGAA TAGATATGAG GACCAATTAG ATGAAAATAT GATTCAGTTT
    TACCTAGCTG AACTGATTTT GGCTGTCCAC AGCGTTCATC AGATGGGCTA TGTACATCGA
    GACATCAAGC CCGAGAACAT CCTCATTGAC CGAACGGGAC ACATCAAGCT GGTGGATTTT
    GGATCAGCTG CTAAAATGAA CTCAAATAAG ATGGTAAATG CCAAACTCCC GGTTGGTACT
    CCAGATTACA TGGCCCCTGA AGTGTTGACT GTGATGAATG GGGATGGAAA AGGTGCCTAT
    AGCCTAGACT GTGATTGGTG GTCAGTGGGA GTTATTGCTT ATGAGATGGT TTATGGAAGA
    TGCCCATTCA CTGAAGGAAC CTCAGCCAAA ACCTTCAATA ACATCATGAA TTTCCAGCGG
    TTCTTGAAGT TTCCAGATGA CCCCAAAGTT AGCAGTGAAT TGCTTGATCT AATCCAGAGT
    TTGTTGTGTG GCCAGAAAGA GAGACTGAAG TTTGAGGGCC TTTGCTGTCA TCCTTTCTTC
    TCCAAAATTG ACTGGATTAA CATTCGTAAC TCTCCTCCCC CCTTCGTTCC CACCCTCAAG
    TCTGATGATG ACACCTCCAA TTTTGATGAA CCAGAGAAGA ATTCGTGGGT TTCATCCTCT
    CCGTGCCAGC TGAACCCCTC AGGTTTCTCG GGCGAAGAAC TGCCGTTTGT GGGGTTTTCG
    TATAGCAAGG CACTGGGAAT TCTTGGTAGA TCTGAGCCTG TTGTATCAAG TCTGGACTCC
    CCTGCCAAGA CTAGCTCCAT GGAAAAGAAA CTCCTTATCA AAAGCAAAGA GCTGCAGGAC
    TCCCAGGACA AGTGTCACAA GATGGAGCAG GAAATGACCC GGTTACATCG GAGAGTGTCA
    GAGGTGGAGG CTGTGCTTAG TCAGAAGGAG GTGGAGCTGA AGGCCTCTGA GACTCAGAGA
    TCCCTCCTGG AGCAGGACCT TGCTACCTAC ATCACAGAAT GCAGTAGCTT AAAGCGAAGT
    TTGGAGCAAG CACGGATGGA GGTGTCCCAA GAGGACGACA AAGCACTGCA GCTCCTCCAT
    GATATCAGAG AGCAGAGCCG GAAGCTCCAA GAAATCAAAG AGCAGGAGTA CCAGGCTCAA
    GTGGAAGAAA TGAGGTTGAT GATGAATCAG TTGGAAGAGG ACCTGGTTTC GGCGAGAAGG
    CGGAGTGACC TCTATGAATC GGAGCTCAGA GAGTCTCGGC TGGCTGCTGA AGAGTTTAAG
    CGGAAAGCAA CAGAATGTCA GCATAAACTG ATGAAGGCTA AAGATCAAGG GAAGCCTGAA
    GTGGGAGAAT ATGCCAAACT GGAGAAGATC AATGCTGAGC AGCAGCTCAA AATTCAGGAG
    CTCCAAGAGA AGCTAGAAAA GGCTGTGAAA GCTAGCACAG AGGCCACCGA GCTGCTGCAG
    AACATCCGTC AGGCGAAGGA GCGGGCCGAG CGTGAGCTGG AGAAGCTGCA CAACCGTGAG
    GACTCCTCCG AGGGCATCAA GAAGAAGCTG GTGGAGGCCG AGGAACGCCG CCACTCTCTG
    GAGAACAAGG TAAAGAGGCT AGAGACCATG GAGCGTAGAG AAAACAGACT GAAGGATGAC
    ATCCAGACAA AGTCCCAACA GATCCAGCAG ATGGCTGATA AAATTCTGGA GCTGGAGGAG
    AAGCACAGAG AAGCCCAGGT CTCAGCGCAG CATCTGGAGG TGCACCTGAA ACAGAAAGAG
    CAGCACTATG AAGAAAAAAT TAAAGTGTTG GACAATCAGA TAAAGAAAGA CCTAGCAGAC
    AAGGAGACAC TGGAGAACCT GATGCAAAGA CACGAAGAGG AGGCCCATGA GAAAGGCAAA
    ATTCTCAGCG AGCAGAAGGC GATGATCAAT GCTATGGATT CCAAGATCAG ATCCCTGGAA
    CAAAGGATAG TGGAATTGTC GGAAGCCAAT AAACTTGCGG CCAACAGCAG TCTCTTTACC
    CAGAGGAACA TGAAGGCCCA GGAAGAGATG ATTTCAGAAC TCAGGCAACA GAAGTTTTAC
    CTGGAGACGC AAGCTGGGAA ATTGGAGGCC CAGAACCGGA AGCTGGAGGA GCAGCTGGAA
    AAAATAAGCC ACCAAGACCA CAGTGACAAG AACCGTCTGC TGGAGCTGGA GACCAGGCTA
    AGGGAGGTCA GTCTAGAACA TGAAGAGCAG AAACTGGAGC TCAAACGCCA GCTCACAGAG
    CTGCAGCTCT CCCTGCAGGA GCGTGAATCC CAGCTGACAG CCCTGCAGGC AGCACGGGCG
    GCCCTGGAGA GCCAGCTCCG CCAGGCCAAG ACGGAGCTGG AAGAGACGAC AGCAGAAGCA
    GAAGAAGAGA TCCAGGCGCT TACGGCACAT AGAGATGAAA TCCAGCGCAA ATTCGATGCC
    CTTCGTAACA GCTGTACTGT GATCACGGAC TTGGAGGAGC AGCTAAACCA GCTGACGGAG
    GACAATGCCG AGCTCAACAA CCAAAACTTC TACTTGTCTA AACAACTCGA TGAGGCCTCT
    GGTGCCAACG ACGAGATTGT GCAGCTGCGA AGTGAAGTGG ACCATCTCCG CCGCGAGATC
    ACTGAGAGGG AGATGCAGCT CACTAGCCAG AAGCAAACGA TGGAGGCGCT GAAGACCACG
    TGCACAATGC TGGAGGAGCA GGTCATGGAC CTGGAGGCCC TGAACGACGA GCTGCTGGAG
    AAGGAGCGGC AGTGGGAGGC CTGGAGGAGT GTCCTTGGTG ACGAGAAGTC CCAGTTTGAG
    TGTCGGGTTC GAGAGTTGCA GAGGATGCTG GACACCGAAA AGCAGAGCAG GGCGAGGGCC
    GACCAGAGGA TCACTGAGTC GCGCCAGGTG GTCGAGCTGG CAGTAAAGGA GCACAAGGCC
    GAGATCCTGG CCTTGCAGCA GGCTCTCAAG GAGCAGAAGC TGAAGGCCGA GAGCCTCTCC
    GACAAGCTCA ATGATCTGGA GAAGAAGCAC GCCATGCTTG AAATGAATGC CCGAAGTTTA
    CAACAGAAGC TGGAGACAGA ACGAGAGCTC AAACAAAGGC TTCTGGAAGA GCAAGCCAAG
    TTACAGCAGC AGATGGACCT GCAGAAGAAT CATATTTTCC GTCTGACTCA AGGGCTGCAA
    GAAGCTCTGG ATCGGGCTGA TCTGCTGAAG ACAGAAAGGA GTGACCTGGA ATATCAGCTG
    GAAAACATTC AGGTTCTCTA TTCTCATGAG AAGGTGAAGA TGGAAGGCAC TATTTCCCAG
    CAAACCAAAC TCATCGATTT TCTGCAAGCC AAAATGGACC AGCCTGCCAA AAAGAAAAAG
    GGTTTATTTA GTCGACGGAA AGAGGACCCT GCTTTGCCCA CACAGGTTCC TCTGCAGTAC
    AATGAGCTGA AGGTGGCTCT GGAGAAGGAG AAAGCTCGCT GCGCGGAGCT CGAGGAAGCC
    CTGCAGAAGA CCCGCATCGA GCTCCGCTCC GCCCGGGAGG AAGCTGCCCA CCGGAAAGCT
    GCAGACCACC CACACCCATC TACGCCGGCT ACCGCCAGGC AGCAGATCGC CATGTCCGCC
    ATTGTGCGGT CACCCGAGCA CCAGCCCAGC GCCCTGAGCC TGCTCGCCCC GCCGTCCAGC
    CGCAGGAAGG AGTCTTCCAC TCCAGAGGAA TTCAGCCGGC GTCTTAAGGA GCGCATGCAC
    CACAACATCC CTCACCGGTT CAACGTGGGA CTGAACATGC GAGCCACGAA GTGCGCCGTG
    TGTCTGGATA CCGTGCACTT CGGGCGCCAG GCATCCAAGT GTCTCGAATG TCAGGTCATG
    TGCCACCCCA AGTGCTCCAC ATGCTTGCCA GCCACCTGTG GCCTGCCAGC CGAATACGCC
    ACACACTTCA CCGAGGCCTT CTGCCGCGAC AAAATGAACT CCCCGGGTCT GCAGTCCAAG
    GAGCCCAGCA GCAGCCTGCA CCTGGAAGGG TGGATGAAGG TGCCCAGGAA TAACAAGCGA
    GGACAGCAAG GCTGGGACAG GAAGTACATC GTTCTGGAGG GCTCCAAAGT CCTCATTTAT
    GACAACGAAG CCAGAGAAGC TGGACAGAGG CCGGTGGAAG AATTTGAGCT GTGCCTTCCC
    GACGGGGACG TGTCTATTCA TGGCGCCGTT GGTGCTTCTG AACTTGCAAA CACAGCCAAA
    GCAGATGTCC CCTATGTCCT GAAGATGGAG TCTCACCCCC ATACCACCTG CTGGCCTGGG
    AGGACCCTCT ACTTGCTGGC TCCCAGCTTT CCCGACAAGC AGCGCTGGGT CACCGCCTTA
    GAGTCAGTTG TGGCAGGTGG GAGAGTGTCT AGGGAAAAGG CGGAAGCCGA CGCTAAATTG
    CTCGGAAACT CCCTGTTGAA ACTGGAAGGT GATGACCGTC TGGACATGAA CTGCACGCTG
    CCCTTCAGCG ACCAGGTGGT GCTGGTGGGC ACCGAGGAAG GGCTGTATGC GCTGAATGTC
    TTGAAGAACT CCCTCACCCA CGTCCCAGGA ATCGGAGCAG TCTTCCAGAT TTACATCATC
    AAGGACCTGG AGAAGCTCCT CATGATCGCA GGAGAGGAGC GGGCCCTGTG TCTCGTGGAT
    GTGAAGAAGG TGAAGCAGTC CCTCGCGCAG TCTCACCTCC CCGCCCAGCC AGACATCTCG
    CCCAACATCT TTGAAGCGGT CAAGGGCTGC CACTTGTTTG CTGCTGGCAA GATTGAGAGC
    GGGCTCTGCA TCTGTGCAGC CATGCCCAGC AAAGTTGTCA TTCTCCGCTA CAATGAAAAC
    CTCAGCAAGT ACTGCATTCG CAAAGAGATC GAGACCTCGG AGCCCTGCAG CTGTATCCAC
    TTCACCAACT ACAGTATCCT CATCGGAACC AATAAATTCT ACGAAATCGA CATGAAGCAG
    TACACGCTAG AGGAATTCCT GGATAAGAAC GACCATTCCT TGGCGCCTGC CGTGTTTGCC
    TCCTCTTCCA ACAGCTTCCC CGTGTCCATC ATGCAGGTGA ACGGCGCGGG GCAGCGGGAG
    GAGTTCCTGC TCTGTTTCCA CGAATTCGGG GTGTTCGTGG ATTCTTATGG AAGACGCAGC
    CGCACAGATG ATCTCAAGTG GAGTCGCTTA CCTCTGGCCT TTGCCTACAG AGAACCCTAT
    CTGTTTGTGA CTCACTTCAA CTCACTCGAA GTAATTGAGA TCCAGGCCCG CTCCTCTCTG
    GGGACCCCCG CCCGAGCATA TTTGGAAATC CCCAACCCAC GCTACCTGGG CCCTGCAATC
    TCCTCAGGAG CCATCTACCT GGCGTCCTCA TATCAGGATA AGTTAAGGGT CATCTGCTGC
    AAAGGAAACC TGGTGAAGGA GACCGGCACC GACCACCACC GTGGCCCATC CACCTCCCGC
    AGCCCCAACA AGCGAGGCCC GCCGACGTAC AACGAGCACA TCACCAAGCG CGTGGCCTCA
    AGCCCGGCGC CTCCCGAAGG CCCCAGCCAC CCTCGAGAGC CAAGCACACC CCACCGCTAC
    CGCGAGGGGC GGACCGAGCT GCGCAGGGAC AAGTCTCCGG GCCGCCCCCT GGAGCGCGAG
    AAGTCCCCCG GCCGGATGCT GAGCACGCGG AGGGAGCGCT CCCCCGGGCG GCTGTTTGAG
    GACAGCAGCA GGGGCCGGCT GCCCGTGGGG GCCGTGAGGA CCCCGCTGTC CCAGGTCAAC
    AAGGTCTGGG ACCAGTCTTC AGTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024833503.1
    CDS113..6319
    Translation

    Target ORF information:

    RefSeq Version XM_024977735.1
    Organism Bos taurus(cattle)
    Definition Bos taurus citron rho-interacting serine/threonine kinase (CIT), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024977735.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    ATGTTGAAGT TCAAGTATGG AGCACGGAAT CTACTGGACG CTGGTACTGT TGAATCCATT 
    GCCAGCCGGG CCTCCAGGCT GAATCTTTTC TTCCAGGGGA AACCACCCTC CATGACTCAA
    CAGCAGATGT CTCTTCTTTC CCGAGAAGCG ATATTAGATG CCCTCTTTGT TCTCTTTAAA
    GAATGCAGTC AGCCAGCTCT GATGAAGATT AAGCACGTGA GCAACTTTGT CCAAAAGTAT
    TCCGATATCA TAGCTGAGTT GCAAGAGCTC CAGCCTTCAG TGAAGGACTT TGAAGTGAAA
    AGTGTCGTAG GCTGCGGTCA CTTTGCTGAA GTACAGGTGG TCAGAGAGAA AGCGACTGGG
    GATGTCTATG CCATGAAAGT CATGAAGAAG AAGGCCTTGT TGGCCCAGGA GCAGGTCTCA
    TATTTTGAGG AAGAACAGAA CATATTATCT CAGAGTACAA GTCCTTGGAT CCCCCAGTTG
    CAGTATGCCT TTCAGGACAA AAATAACCTT TATCTGGTCA TGGAATATCA GCCTGGAGGG
    GATTTGCTGT CACTTTTGAA TAGATATGAG GACCAATTAG ATGAAAATAT GATTCAGTTT
    TACCTAGCTG AACTGATTTT GGCTGTCCAC AGCGTTCATC AGATGGGCTA TGTACATCGA
    GACATCAAGC CCGAGAACAT CCTCATTGAC CGAACGGGAC ACATCAAGCT GGTGGATTTT
    GGATCAGCTG CTAAAATGAA CTCAAATAAG ATGGTAAATG CCAAACTCCC GGTTGGTACT
    CCAGATTACA TGGCCCCTGA AGTGTTGACT GTGATGAATG GGGATGGAAA AGGTGCCTAT
    AGCCTAGACT GTGATTGGTG GTCAGTGGGA GTTATTGCTT ATGAGATGGT TTATGGAAGA
    TGCCCATTCA CTGAAGGAAC CTCAGCCAAA ACCTTCAATA ACATCATGAA TTTCCAGCGG
    TTCTTGAAGT TTCCAGATGA CCCCAAAGTT AGCAGTGAAT TGCTTGATCT AATCCAGAGT
    TTGTTGTGTG GCCAGAAAGA GAGACTGAAG TTTGAGGGCC TTTGCTGTCA TCCTTTCTTC
    TCCAAAATTG ACTGGATTAA CATTCGTAAC TCTCCTCCCC CCTTCGTTCC CACCCTCAAG
    TCTGATGATG ACACCTCCAA TTTTGATGAA CCAGAGAAGA ATTCGTGGGT TTCATCCTCT
    CCGTGCCAGC TGAACCCCTC AGGTTTCTCG GGCGAAGAAC TGCCGTTTGT GGGGTTTTCG
    TATAGCAAGG CACTGGGAAT TCTTGGTAGA TCTGAGCCTG TTGTATCAAG TCTGGACTCC
    CCTGCCAAGA CTAGCTCCAT GGAAAAGAAA CTCCTTATCA AAAGCAAAGA GCTGCAGGAC
    TCCCAGGACA AGTGTCACAA GATGGAGCAG GAAATGACCC GGTTACATCG GAGAGTGTCA
    GAGGTGGAGG CTGTGCTTAG TCAGAAGGAG GTGGAGCTGA AGGCCTCTGA GACTCAGAGA
    TCCCTCCTGG AGCAGGACCT TGCTACCTAC ATCACAGAAT GCAGTAGCTT AAAGCGAAGT
    TTGGAGCAAG CACGGATGGA GGTGTCCCAA GAGGACGACA AAGCACTGCA GCTCCTCCAT
    GATATCAGAG AGCAGAGCCG GAAGCTCCAA GAAATCAAAG AGCAGGAGTA CCAGGCTCAA
    GTGGAAGAAA TGAGGTTGAT GATGAATCAG TTGGAAGAGG ACCTGGTTTC GGCGAGAAGG
    CGGAGTGACC TCTATGAATC GGAGCTCAGA GAGTCTCGGC TGGCTGCTGA AGAGTTTAAG
    CGGAAAGCAA CAGAATGTCA GCATAAACTG ATGAAGGCTA AAGATCAAGG GAAGCCTGAA
    GTGGGAGAAT ATGCCAAACT GGAGAAGATC AATGCTGAGC AGCAGCTCAA AATTCAGGAG
    CTCCAAGAGA AGCTAGAAAA GGCTGTGAAA GCTAGCACAG AGGCCACCGA GCTGCTGCAG
    AACATCCGTC AGGCGAAGGA GCGGGCCGAG CGTGAGCTGG AGAAGCTGCA CAACCGTGAG
    GACTCCTCCG AGGGCATCAA GAAGAAGCTG GTGGAGGCCG AGGAACGCCG CCACTCTCTG
    GAGAACAAGG TAAAGAGGCT AGAGACCATG GAGCGTAGAG AAAACAGACT GAAGGATGAC
    ATCCAGACAA AGTCCCAACA GATCCAGCAG ATGGCTGATA AAATTCTGGA GCTGGAGGAG
    AAGCACAGAG AAGCCCAGGT CTCAGCGCAG CATCTGGAGG TGCACCTGAA ACAGAAAGAG
    CAGCACTATG AAGAAAAAAT TAAAGTGTTG GACAATCAGA TAAAGAAAGA CCTAGCAGAC
    AAGGAGACAC TGGAGAACCT GATGCAAAGA CACGAAGAGG AGGCCCATGA GAAAGGCAAA
    ATTCTCAGCG AGCAGAAGGC GATGATCAAT GCTATGGATT CCAAGATCAG ATCCCTGGAA
    CAAAGGATAG TGGAATTGTC GGAAGCCAAT AAACTTGCGG CCAACAGCAG TCTCTTTACC
    CAGAGGAACA TGAAGGCCCA GGAAGAGATG ATTTCAGAAC TCAGGCAACA GAAGTTTTAC
    CTGGAGACGC AAGCTGGGAA ATTGGAGGCC CAGAACCGGA AGCTGGAGGA GCAGCTGGAA
    AAAATAAGCC ACCAAGACCA CAGTGACAAG AACCGTCTGC TGGAGCTGGA GACCAGGCTA
    AGGGAGGTCA GTCTAGAACA TGAAGAGCAG AAACTGGAGC TCAAACGCCA GCTCACAGAG
    CTGCAGCTCT CCCTGCAGGA GCGTGAATCC CAGCTGACAG CCCTGCAGGC AGCACGGGCG
    GCCCTGGAGA GCCAGCTCCG CCAGGCCAAG ACGGAGCTGG AAGAGACGAC AGCAGAAGCA
    GAAGAAGAGA TCCAGGCGCT TACGGCACAT AGAGATGAAA TCCAGCGCAA ATTCGATGCC
    CTTCGTAACA GCTGTACTGT GATCACGGAC TTGGAGGAGC AGCTAAACCA GCTGACGGAG
    GACAATGCCG AGCTCAACAA CCAAAACTTC TACTTGTCTA AACAACTCGA TGAGGCCTCT
    GGTGCCAACG ACGAGATTGT GCAGCTGCGA AGTGAAGTGG ACCATCTCCG CCGCGAGATC
    ACTGAGAGGG AGATGCAGCT CACTAGCCAG AAGCAAACGA TGGAGGCGCT GAAGACCACG
    TGCACAATGC TGGAGGAGCA GGTCATGGAC CTGGAGGCCC TGAACGACGA GCTGCTGGAG
    AAGGAGCGGC AGTGGGAGGC CTGGAGGAGT GTCCTTGGTG ACGAGAAGTC CCAGTTTGAG
    TGTCGGGTTC GAGAGTTGCA GAGGATGCTG GACACCGAAA AGCAGAGCAG GGCGAGGGCC
    GACCAGAGGA TCACTGAGTC GCGCCAGGTG GTCGAGCTGG CAGTAAAGGA GCACAAGGCC
    GAGATCCTGG CCTTGCAGCA GGCTCTCAAG GAGCAGAAGC TGAAGGCCGA GAGCCTCTCC
    GACAAGCTCA ATGATCTGGA GAAGAAGCAC GCCATGCTTG AAATGAATGC CCGAAGTTTA
    CAACAGAAGC TGGAGACAGA ACGAGAGCTC AAACAAAGGC TTCTGGAAGA GCAAGCCAAG
    TTACAGCAGC AGATGGACCT GCAGAAGAAT CATATTTTCC GTCTGACTCA AGGGCTGCAA
    GAAGCTCTGG ATCGGGCTGA TCTGCTGAAG ACAGAAAGGA GTGACCTGGA ATATCAGCTG
    GAAAACATTC AGGTTCTCTA TTCTCATGAG AAGGTGAAGA TGGAAGGCAC TATTTCCCAG
    CAAACCAAAC TCATCGATTT TCTGCAAGCC AAAATGGACC AGCCTGCCAA AAAGAAAAAG
    GGTTTATTTA GTCGACGGAA AGAGGACCCT GCTTTGCCCA CACAGGTTCC TCTGCAGTAC
    AATGAGCTGA AGGTGGCTCT GGAGAAGGAG AAAGCTCGCT GCGCGGAGCT CGAGGAAGCC
    CTGCAGAAGA CCCGCATCGA GCTCCGCTCC GCCCGGGAGG AAGCTGCCCA CCGGAAAGCT
    GCAGACCACC CACACCCATC TACGCCGGCT ACCGCCAGGC AGCAGATCGC CATGTCCGCC
    ATTGTGCGGT CACCCGAGCA CCAGCCCAGC GCCCTGAGCC TGCTCGCCCC GCCGTCCAGC
    CGCAGGAAGG AGTCTTCCAC TCCAGAGGAA TTCAGCCGGC GTCTTAAGGA GCGCATGCAC
    CACAACATCC CTCACCGGTT CAACGTGGGA CTGAACATGC GAGCCACGAA GTGCGCCGTG
    TGTCTGGATA CCGTGCACTT CGGGCGCCAG GCATCCAAGT GTCTCGAATG TCAGGTCATG
    TGCCACCCCA AGTGCTCCAC ATGCTTGCCA GCCACCTGTG GCCTGCCAGC CGAATACGCC
    ACACACTTCA CCGAGGCCTT CTGCCGCGAC AAAATGAACT CCCCGGGTCT GCAGTCCAAG
    GAGCCCAGCA GCAGCCTGCA CCTGGAAGGG TGGATGAAGG TGCCCAGGAA TAACAAGCGA
    GGACAGCAAG GCTGGGACAG GAAGTACATC GTTCTGGAGG GCTCCAAAGT CCTCATTTAT
    GACAACGAAG CCAGAGAAGC TGGACAGAGG CCGGTGGAAG AATTTGAGCT GTGCCTTCCC
    GACGGGGACG TGTCTATTCA TGGCGCCGTT GGTGCTTCTG AACTTGCAAA CACAGCCAAA
    GCAGATGTCC CCTATGTCCT GAAGATGGAG TCTCACCCCC ATACCACCTG CTGGCCTGGG
    AGGACCCTCT ACTTGCTGGC TCCCAGCTTT CCCGACAAGC AGCGCTGGGT CACCGCCTTA
    GAGTCAGTTG TGGCAGGTGG GAGAGTGTCT AGGGAAAAGG CGGAAGCCGA CGCTAAATTG
    CTCGGAAACT CCCTGTTGAA ACTGGAAGGT GATGACCGTC TGGACATGAA CTGCACGCTG
    CCCTTCAGCG ACCAGGTGGT GCTGGTGGGC ACCGAGGAAG GGCTGTATGC GCTGAATGTC
    TTGAAGAACT CCCTCACCCA CGTCCCAGGA ATCGGAGCAG TCTTCCAGAT TTACATCATC
    AAGGACCTGG AGAAGCTCCT CATGATCGCA GGAGAGGAGC GGGCCCTGTG TCTCGTGGAT
    GTGAAGAAGG TGAAGCAGTC CCTCGCGCAG TCTCACCTCC CCGCCCAGCC AGACATCTCG
    CCCAACATCT TTGAAGCGGT CAAGGGCTGC CACTTGTTTG CTGCTGGCAA GATTGAGAGC
    GGGCTCTGCA TCTGTGCAGC CATGCCCAGC AAAGTTGTCA TTCTCCGCTA CAATGAAAAC
    CTCAGCAAGT ACTGCATTCG CAAAGAGATC GAGACCTCGG AGCCCTGCAG CTGTATCCAC
    TTCACCAACT ACAGTATCCT CATCGGAACC AATAAATTCT ACGAAATCGA CATGAAGCAG
    TACACGCTAG AGGAATTCCT GGATAAGAAC GACCATTCCT TGGCGCCTGC CGTGTTTGCC
    TCCTCTTCCA ACAGCTTCCC CGTGTCCATC ATGCAGGTGA ACGGCGCGGG GCAGCGGGAG
    GAGTTCCTGC TCTGTTTCCA CGAATTCGGG GTGTTCGTGG ATTCTTATGG AAGACGCAGC
    CGCACAGATG ATCTCAAGTG GAGTCGCTTA CCTCTGGCCT TTGCCTACAG AGAACCCTAT
    CTGTTTGTGA CTCACTTCAA CTCACTCGAA GTAATTGAGA TCCAGGCCCG CTCCTCTCTG
    GGGACCCCCG CCCGAGCATA TTTGGAAATC CCCAACCCAC GCTACCTGGG CCCTGCAATC
    TCCTCAGGAG CCATCTACCT GGCGTCCTCA TATCAGGATA AGTTAAGGGT CATCTGCTGC
    AAAGGAAACC TGGTGAAGGA GACCGGCACC GACCACCACC GTGGCCCATC CACCTCCCGC
    AGCCCCAACA AGCGAGGCCC GCCGACGTAC AACGAGCACA TCACCAAGCG CGTGGCCTCA
    AGCCCGGCGC CTCCCGAAGG CCCCAGCCAC CCTCGAGAGC CAAGCACACC CCACCGCTAC
    CGCGAGGGGC GGACCGAGCT GCGCAGGGAC AAGTCTCCGG GCCGCCCCCT GGAGCGCGAG
    AAGTCCCCCG GCCGGATGCT GAGCACGCGG AGGGAGCGCT CCCCCGGGCG GCTGTTTGAG
    GACAGCAGCA GGGGCCGGCT GCCCGTGGGG GCCGTGAGGA CCCCGCTGTC CCAGGTCAAC
    AAGGTCTGGG ACCAGTCTTC AGTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa224624
    Clone ID Related Accession (Same CDS sequence) XM_024977736.1
    Accession Version XM_024977736.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6207bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product citron Rho-interacting kinase isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    ATGTTGAAGT TCAAGTATGG AGCACGGAAT CTACTGGACG CTGGTACTGT TGAATCCATT 
    GCCAGCCGGG CCTCCAGGCT GAATCTTTTC TTCCAGGGGA AACCACCCTC CATGACTCAA
    CAGCAGATGT CTCTTCTTTC CCGAGAAGCG ATATTAGATG CCCTCTTTGT TCTCTTTAAA
    GAATGCAGTC AGCCAGCTCT GATGAAGATT AAGCACGTGA GCAACTTTGT CCAAAAGTAT
    TCCGATATCA TAGCTGAGTT GCAAGAGCTC CAGCCTTCAG TGAAGGACTT TGAAGTGAAA
    AGTGTCGTAG GCTGCGGTCA CTTTGCTGAA GTACAGGTGG TCAGAGAGAA AGCGACTGGG
    GATGTCTATG CCATGAAAGT CATGAAGAAG AAGGCCTTGT TGGCCCAGGA GCAGGTCTCA
    TATTTTGAGG AAGAACAGAA CATATTATCT CAGAGTACAA GTCCTTGGAT CCCCCAGTTG
    CAGTATGCCT TTCAGGACAA AAATAACCTT TATCTGGTCA TGGAATATCA GCCTGGAGGG
    GATTTGCTGT CACTTTTGAA TAGATATGAG GACCAATTAG ATGAAAATAT GATTCAGTTT
    TACCTAGCTG AACTGATTTT GGCTGTCCAC AGCGTTCATC AGATGGGCTA TGTACATCGA
    GACATCAAGC CCGAGAACAT CCTCATTGAC CGAACGGGAC ACATCAAGCT GGTGGATTTT
    GGATCAGCTG CTAAAATGAA CTCAAATAAG ATGGTAAATG CCAAACTCCC GGTTGGTACT
    CCAGATTACA TGGCCCCTGA AGTGTTGACT GTGATGAATG GGGATGGAAA AGGTGCCTAT
    AGCCTAGACT GTGATTGGTG GTCAGTGGGA GTTATTGCTT ATGAGATGGT TTATGGAAGA
    TGCCCATTCA CTGAAGGAAC CTCAGCCAAA ACCTTCAATA ACATCATGAA TTTCCAGCGG
    TTCTTGAAGT TTCCAGATGA CCCCAAAGTT AGCAGTGAAT TGCTTGATCT AATCCAGAGT
    TTGTTGTGTG GCCAGAAAGA GAGACTGAAG TTTGAGGGCC TTTGCTGTCA TCCTTTCTTC
    TCCAAAATTG ACTGGATTAA CATTCGTAAC TCTCCTCCCC CCTTCGTTCC CACCCTCAAG
    TCTGATGATG ACACCTCCAA TTTTGATGAA CCAGAGAAGA ATTCGTGGGT TTCATCCTCT
    CCGTGCCAGC TGAACCCCTC AGGTTTCTCG GGCGAAGAAC TGCCGTTTGT GGGGTTTTCG
    TATAGCAAGG CACTGGGAAT TCTTGGTAGA TCTGAGCCTG TTGTATCAAG TCTGGACTCC
    CCTGCCAAGA CTAGCTCCAT GGAAAAGAAA CTCCTTATCA AAAGCAAAGA GCTGCAGGAC
    TCCCAGGACA AGTGTCACAA GATGGAGCAG GAAATGACCC GGTTACATCG GAGAGTGTCA
    GAGGTGGAGG CTGTGCTTAG TCAGAAGGAG GTGGAGCTGA AGGCCTCTGA GACTCAGAGA
    TCCCTCCTGG AGCAGGACCT TGCTACCTAC ATCACAGAAT GCAGTAGCTT AAAGCGAAGT
    TTGGAGCAAG CACGGATGGA GGTGTCCCAA GAGGACGACA AAGCACTGCA GCTCCTCCAT
    GATATCAGAG AGCAGAGCCG GAAGCTCCAA GAAATCAAAG AGCAGGAGTA CCAGGCTCAA
    GTGGAAGAAA TGAGGTTGAT GATGAATCAG TTGGAAGAGG ACCTGGTTTC GGCGAGAAGG
    CGGAGTGACC TCTATGAATC GGAGCTCAGA GAGTCTCGGC TGGCTGCTGA AGAGTTTAAG
    CGGAAAGCAA CAGAATGTCA GCATAAACTG ATGAAGGCTA AAGATCAAGG GAAGCCTGAA
    GTGGGAGAAT ATGCCAAACT GGAGAAGATC AATGCTGAGC AGCAGCTCAA AATTCAGGAG
    CTCCAAGAGA AGCTAGAAAA GGCTGTGAAA GCTAGCACAG AGGCCACCGA GCTGCTGCAG
    AACATCCGTC AGGCGAAGGA GCGGGCCGAG CGTGAGCTGG AGAAGCTGCA CAACCGTGAG
    GACTCCTCCG AGGGCATCAA GAAGAAGCTG GTGGAGGCCG AGGAACGCCG CCACTCTCTG
    GAGAACAAGG TAAAGAGGCT AGAGACCATG GAGCGTAGAG AAAACAGACT GAAGGATGAC
    ATCCAGACAA AGTCCCAACA GATCCAGCAG ATGGCTGATA AAATTCTGGA GCTGGAGGAG
    AAGCACAGAG AAGCCCAGGT CTCAGCGCAG CATCTGGAGG TGCACCTGAA ACAGAAAGAG
    CAGCACTATG AAGAAAAAAT TAAAGTGTTG GACAATCAGA TAAAGAAAGA CCTAGCAGAC
    AAGGAGACAC TGGAGAACCT GATGCAAAGA CACGAAGAGG AGGCCCATGA GAAAGGCAAA
    ATTCTCAGCG AGCAGAAGGC GATGATCAAT GCTATGGATT CCAAGATCAG ATCCCTGGAA
    CAAAGGATAG TGGAATTGTC GGAAGCCAAT AAACTTGCGG CCAACAGCAG TCTCTTTACC
    CAGAGGAACA TGAAGGCCCA GGAAGAGATG ATTTCAGAAC TCAGGCAACA GAAGTTTTAC
    CTGGAGACGC AAGCTGGGAA ATTGGAGGCC CAGAACCGGA AGCTGGAGGA GCAGCTGGAA
    AAAATAAGCC ACCAAGACCA CAGTGACAAG AACCGTCTGC TGGAGCTGGA GACCAGGCTA
    AGGGAGGTCA GTCTAGAACA TGAAGAGCAG AAACTGGAGC TCAAACGCCA GCTCACAGAG
    CTGCAGCTCT CCCTGCAGGA GCGTGAATCC CAGCTGACAG CCCTGCAGGC AGCACGGGCG
    GCCCTGGAGA GCCAGCTCCG CCAGGCCAAG ACGGAGCTGG AAGAGACGAC AGCAGAAGCA
    GAAGAAGAGA TCCAGGCGCT TACGGCACAT AGAGATGAAA TCCAGCGCAA ATTCGATGCC
    CTTCGTAACA GCTGTACTGT GATCACGGAC TTGGAGGAGC AGCTAAACCA GCTGACGGAG
    GACAATGCCG AGCTCAACAA CCAAAACTTC TACTTGTCTA AACAACTCGA TGAGGCCTCT
    GGTGCCAACG ACGAGATTGT GCAGCTGCGA AGTGAAGTGG ACCATCTCCG CCGCGAGATC
    ACTGAGAGGG AGATGCAGCT CACTAGCCAG AAGCAAACGA TGGAGGCGCT GAAGACCACG
    TGCACAATGC TGGAGGAGCA GGTCATGGAC CTGGAGGCCC TGAACGACGA GCTGCTGGAG
    AAGGAGCGGC AGTGGGAGGC CTGGAGGAGT GTCCTTGGTG ACGAGAAGTC CCAGTTTGAG
    TGTCGGGTTC GAGAGTTGCA GAGGATGCTG GACACCGAAA AGCAGAGCAG GGCGAGGGCC
    GACCAGAGGA TCACTGAGTC GCGCCAGGTG GTCGAGCTGG CAGTAAAGGA GCACAAGGCC
    GAGATCCTGG CCTTGCAGCA GGCTCTCAAG GAGCAGAAGC TGAAGGCCGA GAGCCTCTCC
    GACAAGCTCA ATGATCTGGA GAAGAAGCAC GCCATGCTTG AAATGAATGC CCGAAGTTTA
    CAACAGAAGC TGGAGACAGA ACGAGAGCTC AAACAAAGGC TTCTGGAAGA GCAAGCCAAG
    TTACAGCAGC AGATGGACCT GCAGAAGAAT CATATTTTCC GTCTGACTCA AGGGCTGCAA
    GAAGCTCTGG ATCGGGCTGA TCTGCTGAAG ACAGAAAGGA GTGACCTGGA ATATCAGCTG
    GAAAACATTC AGGTTCTCTA TTCTCATGAG AAGGTGAAGA TGGAAGGCAC TATTTCCCAG
    CAAACCAAAC TCATCGATTT TCTGCAAGCC AAAATGGACC AGCCTGCCAA AAAGAAAAAG
    GTTCCTCTGC AGTACAATGA GCTGAAGGTG GCTCTGGAGA AGGAGAAAGC TCGCTGCGCG
    GAGCTCGAGG AAGCCCTGCA GAAGACCCGC ATCGAGCTCC GCTCCGCCCG GGAGGAAGCT
    GCCCACCGGA AAGCTGCAGA CCACCCACAC CCATCTACGC CGGCTACCGC CAGGCAGCAG
    ATCGCCATGT CCGCCATTGT GCGGTCACCC GAGCACCAGC CCAGCGCCCT GAGCCTGCTC
    GCCCCGCCGT CCAGCCGCAG GAAGGAGTCT TCCACTCCAG AGGAATTCAG CCGGCGTCTT
    AAGGAGCGCA TGCACCACAA CATCCCTCAC CGGTTCAACG TGGGACTGAA CATGCGAGCC
    ACGAAGTGCG CCGTGTGTCT GGATACCGTG CACTTCGGGC GCCAGGCATC CAAGTGTCTC
    GAATGTCAGG TCATGTGCCA CCCCAAGTGC TCCACATGCT TGCCAGCCAC CTGTGGCCTG
    CCAGCCGAAT ACGCCACACA CTTCACCGAG GCCTTCTGCC GCGACAAAAT GAACTCCCCG
    GGTCTGCAGT CCAAGGAGCC CAGCAGCAGC CTGCACCTGG AAGGGTGGAT GAAGGTGCCC
    AGGAATAACA AGCGAGGACA GCAAGGCTGG GACAGGAAGT ACATCGTTCT GGAGGGCTCC
    AAAGTCCTCA TTTATGACAA CGAAGCCAGA GAAGCTGGAC AGAGGCCGGT GGAAGAATTT
    GAGCTGTGCC TTCCCGACGG GGACGTGTCT ATTCATGGCG CCGTTGGTGC TTCTGAACTT
    GCAAACACAG CCAAAGCAGA TGTCCCCTAT GTCCTGAAGA TGGAGTCTCA CCCCCATACC
    ACCTGCTGGC CTGGGAGGAC CCTCTACTTG CTGGCTCCCA GCTTTCCCGA CAAGCAGCGC
    TGGGTCACCG CCTTAGAGTC AGTTGTGGCA GGTGGGAGAG TGTCTAGGGA AAAGGCGGAA
    GCCGACGCTG GCCGTGACTG TACTTCCTAC GAGCCTCTGC CCGCGTGGGC TCAGAAATTG
    CTCGGAAACT CCCTGTTGAA ACTGGAAGGT GATGACCGTC TGGACATGAA CTGCACGCTG
    CCCTTCAGCG ACCAGGTGGT GCTGGTGGGC ACCGAGGAAG GGCTGTATGC GCTGAATGTC
    TTGAAGAACT CCCTCACCCA CGTCCCAGGA ATCGGAGCAG TCTTCCAGAT TTACATCATC
    AAGGACCTGG AGAAGCTCCT CATGATCGCA GGAGAGGAGC GGGCCCTGTG TCTCGTGGAT
    GTGAAGAAGG TGAAGCAGTC CCTCGCGCAG TCTCACCTCC CCGCCCAGCC AGACATCTCG
    CCCAACATCT TTGAAGCGGT CAAGGGCTGC CACTTGTTTG CTGCTGGCAA GATTGAGAGC
    GGGCTCTGCA TCTGTGCAGC CATGCCCAGC AAAGTTGTCA TTCTCCGCTA CAATGAAAAC
    CTCAGCAAGT ACTGCATTCG CAAAGAGATC GAGACCTCGG AGCCCTGCAG CTGTATCCAC
    TTCACCAACT ACAGTATCCT CATCGGAACC AATAAATTCT ACGAAATCGA CATGAAGCAG
    TACACGCTAG AGGAATTCCT GGATAAGAAC GACCATTCCT TGGCGCCTGC CGTGTTTGCC
    TCCTCTTCCA ACAGCTTCCC CGTGTCCATC ATGCAGGTGA ACGGCGCGGG GCAGCGGGAG
    GAGTTCCTGC TCTGTTTCCA CGAATTCGGG GTGTTCGTGG ATTCTTATGG AAGACGCAGC
    CGCACAGATG ATCTCAAGTG GAGTCGCTTA CCTCTGGCCT TTGCCTACAG AGAACCCTAT
    CTGTTTGTGA CTCACTTCAA CTCACTCGAA GTAATTGAGA TCCAGGCCCG CTCCTCTCTG
    GGGACCCCCG CCCGAGCATA TTTGGAAATC CCCAACCCAC GCTACCTGGG CCCTGCAATC
    TCCTCAGGAG CCATCTACCT GGCGTCCTCA TATCAGGATA AGTTAAGGGT CATCTGCTGC
    AAAGGAAACC TGGTGAAGGA GACCGGCACC GACCACCACC GTGGCCCATC CACCTCCCGC
    AGCCCCAACA AGCGAGGCCC GCCGACGTAC AACGAGCACA TCACCAAGCG CGTGGCCTCA
    AGCCCGGCGC CTCCCGAAGG CCCCAGCCAC CCTCGAGAGC CAAGCACACC CCACCGCTAC
    CGCGAGGGGC GGACCGAGCT GCGCAGGGAC AAGTCTCCGG GCCGCCCCCT GGAGCGCGAG
    AAGTCCCCCG GCCGGATGCT GAGCACGCGG AGGGAGCGCT CCCCCGGGCG GCTGTTTGAG
    GACAGCAGCA GGGGCCGGCT GCCCGTGGGG GCCGTGAGGA CCCCGCTGTC CCAGGTCAAC
    AAGGTCTGGG ACCAGTCTTC AGTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024833504.1
    CDS113..6319
    Translation

    Target ORF information:

    RefSeq Version XM_024977736.1
    Organism Bos taurus(cattle)
    Definition Bos taurus citron rho-interacting serine/threonine kinase (CIT), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024977736.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    ATGTTGAAGT TCAAGTATGG AGCACGGAAT CTACTGGACG CTGGTACTGT TGAATCCATT 
    GCCAGCCGGG CCTCCAGGCT GAATCTTTTC TTCCAGGGGA AACCACCCTC CATGACTCAA
    CAGCAGATGT CTCTTCTTTC CCGAGAAGCG ATATTAGATG CCCTCTTTGT TCTCTTTAAA
    GAATGCAGTC AGCCAGCTCT GATGAAGATT AAGCACGTGA GCAACTTTGT CCAAAAGTAT
    TCCGATATCA TAGCTGAGTT GCAAGAGCTC CAGCCTTCAG TGAAGGACTT TGAAGTGAAA
    AGTGTCGTAG GCTGCGGTCA CTTTGCTGAA GTACAGGTGG TCAGAGAGAA AGCGACTGGG
    GATGTCTATG CCATGAAAGT CATGAAGAAG AAGGCCTTGT TGGCCCAGGA GCAGGTCTCA
    TATTTTGAGG AAGAACAGAA CATATTATCT CAGAGTACAA GTCCTTGGAT CCCCCAGTTG
    CAGTATGCCT TTCAGGACAA AAATAACCTT TATCTGGTCA TGGAATATCA GCCTGGAGGG
    GATTTGCTGT CACTTTTGAA TAGATATGAG GACCAATTAG ATGAAAATAT GATTCAGTTT
    TACCTAGCTG AACTGATTTT GGCTGTCCAC AGCGTTCATC AGATGGGCTA TGTACATCGA
    GACATCAAGC CCGAGAACAT CCTCATTGAC CGAACGGGAC ACATCAAGCT GGTGGATTTT
    GGATCAGCTG CTAAAATGAA CTCAAATAAG ATGGTAAATG CCAAACTCCC GGTTGGTACT
    CCAGATTACA TGGCCCCTGA AGTGTTGACT GTGATGAATG GGGATGGAAA AGGTGCCTAT
    AGCCTAGACT GTGATTGGTG GTCAGTGGGA GTTATTGCTT ATGAGATGGT TTATGGAAGA
    TGCCCATTCA CTGAAGGAAC CTCAGCCAAA ACCTTCAATA ACATCATGAA TTTCCAGCGG
    TTCTTGAAGT TTCCAGATGA CCCCAAAGTT AGCAGTGAAT TGCTTGATCT AATCCAGAGT
    TTGTTGTGTG GCCAGAAAGA GAGACTGAAG TTTGAGGGCC TTTGCTGTCA TCCTTTCTTC
    TCCAAAATTG ACTGGATTAA CATTCGTAAC TCTCCTCCCC CCTTCGTTCC CACCCTCAAG
    TCTGATGATG ACACCTCCAA TTTTGATGAA CCAGAGAAGA ATTCGTGGGT TTCATCCTCT
    CCGTGCCAGC TGAACCCCTC AGGTTTCTCG GGCGAAGAAC TGCCGTTTGT GGGGTTTTCG
    TATAGCAAGG CACTGGGAAT TCTTGGTAGA TCTGAGCCTG TTGTATCAAG TCTGGACTCC
    CCTGCCAAGA CTAGCTCCAT GGAAAAGAAA CTCCTTATCA AAAGCAAAGA GCTGCAGGAC
    TCCCAGGACA AGTGTCACAA GATGGAGCAG GAAATGACCC GGTTACATCG GAGAGTGTCA
    GAGGTGGAGG CTGTGCTTAG TCAGAAGGAG GTGGAGCTGA AGGCCTCTGA GACTCAGAGA
    TCCCTCCTGG AGCAGGACCT TGCTACCTAC ATCACAGAAT GCAGTAGCTT AAAGCGAAGT
    TTGGAGCAAG CACGGATGGA GGTGTCCCAA GAGGACGACA AAGCACTGCA GCTCCTCCAT
    GATATCAGAG AGCAGAGCCG GAAGCTCCAA GAAATCAAAG AGCAGGAGTA CCAGGCTCAA
    GTGGAAGAAA TGAGGTTGAT GATGAATCAG TTGGAAGAGG ACCTGGTTTC GGCGAGAAGG
    CGGAGTGACC TCTATGAATC GGAGCTCAGA GAGTCTCGGC TGGCTGCTGA AGAGTTTAAG
    CGGAAAGCAA CAGAATGTCA GCATAAACTG ATGAAGGCTA AAGATCAAGG GAAGCCTGAA
    GTGGGAGAAT ATGCCAAACT GGAGAAGATC AATGCTGAGC AGCAGCTCAA AATTCAGGAG
    CTCCAAGAGA AGCTAGAAAA GGCTGTGAAA GCTAGCACAG AGGCCACCGA GCTGCTGCAG
    AACATCCGTC AGGCGAAGGA GCGGGCCGAG CGTGAGCTGG AGAAGCTGCA CAACCGTGAG
    GACTCCTCCG AGGGCATCAA GAAGAAGCTG GTGGAGGCCG AGGAACGCCG CCACTCTCTG
    GAGAACAAGG TAAAGAGGCT AGAGACCATG GAGCGTAGAG AAAACAGACT GAAGGATGAC
    ATCCAGACAA AGTCCCAACA GATCCAGCAG ATGGCTGATA AAATTCTGGA GCTGGAGGAG
    AAGCACAGAG AAGCCCAGGT CTCAGCGCAG CATCTGGAGG TGCACCTGAA ACAGAAAGAG
    CAGCACTATG AAGAAAAAAT TAAAGTGTTG GACAATCAGA TAAAGAAAGA CCTAGCAGAC
    AAGGAGACAC TGGAGAACCT GATGCAAAGA CACGAAGAGG AGGCCCATGA GAAAGGCAAA
    ATTCTCAGCG AGCAGAAGGC GATGATCAAT GCTATGGATT CCAAGATCAG ATCCCTGGAA
    CAAAGGATAG TGGAATTGTC GGAAGCCAAT AAACTTGCGG CCAACAGCAG TCTCTTTACC
    CAGAGGAACA TGAAGGCCCA GGAAGAGATG ATTTCAGAAC TCAGGCAACA GAAGTTTTAC
    CTGGAGACGC AAGCTGGGAA ATTGGAGGCC CAGAACCGGA AGCTGGAGGA GCAGCTGGAA
    AAAATAAGCC ACCAAGACCA CAGTGACAAG AACCGTCTGC TGGAGCTGGA GACCAGGCTA
    AGGGAGGTCA GTCTAGAACA TGAAGAGCAG AAACTGGAGC TCAAACGCCA GCTCACAGAG
    CTGCAGCTCT CCCTGCAGGA GCGTGAATCC CAGCTGACAG CCCTGCAGGC AGCACGGGCG
    GCCCTGGAGA GCCAGCTCCG CCAGGCCAAG ACGGAGCTGG AAGAGACGAC AGCAGAAGCA
    GAAGAAGAGA TCCAGGCGCT TACGGCACAT AGAGATGAAA TCCAGCGCAA ATTCGATGCC
    CTTCGTAACA GCTGTACTGT GATCACGGAC TTGGAGGAGC AGCTAAACCA GCTGACGGAG
    GACAATGCCG AGCTCAACAA CCAAAACTTC TACTTGTCTA AACAACTCGA TGAGGCCTCT
    GGTGCCAACG ACGAGATTGT GCAGCTGCGA AGTGAAGTGG ACCATCTCCG CCGCGAGATC
    ACTGAGAGGG AGATGCAGCT CACTAGCCAG AAGCAAACGA TGGAGGCGCT GAAGACCACG
    TGCACAATGC TGGAGGAGCA GGTCATGGAC CTGGAGGCCC TGAACGACGA GCTGCTGGAG
    AAGGAGCGGC AGTGGGAGGC CTGGAGGAGT GTCCTTGGTG ACGAGAAGTC CCAGTTTGAG
    TGTCGGGTTC GAGAGTTGCA GAGGATGCTG GACACCGAAA AGCAGAGCAG GGCGAGGGCC
    GACCAGAGGA TCACTGAGTC GCGCCAGGTG GTCGAGCTGG CAGTAAAGGA GCACAAGGCC
    GAGATCCTGG CCTTGCAGCA GGCTCTCAAG GAGCAGAAGC TGAAGGCCGA GAGCCTCTCC
    GACAAGCTCA ATGATCTGGA GAAGAAGCAC GCCATGCTTG AAATGAATGC CCGAAGTTTA
    CAACAGAAGC TGGAGACAGA ACGAGAGCTC AAACAAAGGC TTCTGGAAGA GCAAGCCAAG
    TTACAGCAGC AGATGGACCT GCAGAAGAAT CATATTTTCC GTCTGACTCA AGGGCTGCAA
    GAAGCTCTGG ATCGGGCTGA TCTGCTGAAG ACAGAAAGGA GTGACCTGGA ATATCAGCTG
    GAAAACATTC AGGTTCTCTA TTCTCATGAG AAGGTGAAGA TGGAAGGCAC TATTTCCCAG
    CAAACCAAAC TCATCGATTT TCTGCAAGCC AAAATGGACC AGCCTGCCAA AAAGAAAAAG
    GTTCCTCTGC AGTACAATGA GCTGAAGGTG GCTCTGGAGA AGGAGAAAGC TCGCTGCGCG
    GAGCTCGAGG AAGCCCTGCA GAAGACCCGC ATCGAGCTCC GCTCCGCCCG GGAGGAAGCT
    GCCCACCGGA AAGCTGCAGA CCACCCACAC CCATCTACGC CGGCTACCGC CAGGCAGCAG
    ATCGCCATGT CCGCCATTGT GCGGTCACCC GAGCACCAGC CCAGCGCCCT GAGCCTGCTC
    GCCCCGCCGT CCAGCCGCAG GAAGGAGTCT TCCACTCCAG AGGAATTCAG CCGGCGTCTT
    AAGGAGCGCA TGCACCACAA CATCCCTCAC CGGTTCAACG TGGGACTGAA CATGCGAGCC
    ACGAAGTGCG CCGTGTGTCT GGATACCGTG CACTTCGGGC GCCAGGCATC CAAGTGTCTC
    GAATGTCAGG TCATGTGCCA CCCCAAGTGC TCCACATGCT TGCCAGCCAC CTGTGGCCTG
    CCAGCCGAAT ACGCCACACA CTTCACCGAG GCCTTCTGCC GCGACAAAAT GAACTCCCCG
    GGTCTGCAGT CCAAGGAGCC CAGCAGCAGC CTGCACCTGG AAGGGTGGAT GAAGGTGCCC
    AGGAATAACA AGCGAGGACA GCAAGGCTGG GACAGGAAGT ACATCGTTCT GGAGGGCTCC
    AAAGTCCTCA TTTATGACAA CGAAGCCAGA GAAGCTGGAC AGAGGCCGGT GGAAGAATTT
    GAGCTGTGCC TTCCCGACGG GGACGTGTCT ATTCATGGCG CCGTTGGTGC TTCTGAACTT
    GCAAACACAG CCAAAGCAGA TGTCCCCTAT GTCCTGAAGA TGGAGTCTCA CCCCCATACC
    ACCTGCTGGC CTGGGAGGAC CCTCTACTTG CTGGCTCCCA GCTTTCCCGA CAAGCAGCGC
    TGGGTCACCG CCTTAGAGTC AGTTGTGGCA GGTGGGAGAG TGTCTAGGGA AAAGGCGGAA
    GCCGACGCTG GCCGTGACTG TACTTCCTAC GAGCCTCTGC CCGCGTGGGC TCAGAAATTG
    CTCGGAAACT CCCTGTTGAA ACTGGAAGGT GATGACCGTC TGGACATGAA CTGCACGCTG
    CCCTTCAGCG ACCAGGTGGT GCTGGTGGGC ACCGAGGAAG GGCTGTATGC GCTGAATGTC
    TTGAAGAACT CCCTCACCCA CGTCCCAGGA ATCGGAGCAG TCTTCCAGAT TTACATCATC
    AAGGACCTGG AGAAGCTCCT CATGATCGCA GGAGAGGAGC GGGCCCTGTG TCTCGTGGAT
    GTGAAGAAGG TGAAGCAGTC CCTCGCGCAG TCTCACCTCC CCGCCCAGCC AGACATCTCG
    CCCAACATCT TTGAAGCGGT CAAGGGCTGC CACTTGTTTG CTGCTGGCAA GATTGAGAGC
    GGGCTCTGCA TCTGTGCAGC CATGCCCAGC AAAGTTGTCA TTCTCCGCTA CAATGAAAAC
    CTCAGCAAGT ACTGCATTCG CAAAGAGATC GAGACCTCGG AGCCCTGCAG CTGTATCCAC
    TTCACCAACT ACAGTATCCT CATCGGAACC AATAAATTCT ACGAAATCGA CATGAAGCAG
    TACACGCTAG AGGAATTCCT GGATAAGAAC GACCATTCCT TGGCGCCTGC CGTGTTTGCC
    TCCTCTTCCA ACAGCTTCCC CGTGTCCATC ATGCAGGTGA ACGGCGCGGG GCAGCGGGAG
    GAGTTCCTGC TCTGTTTCCA CGAATTCGGG GTGTTCGTGG ATTCTTATGG AAGACGCAGC
    CGCACAGATG ATCTCAAGTG GAGTCGCTTA CCTCTGGCCT TTGCCTACAG AGAACCCTAT
    CTGTTTGTGA CTCACTTCAA CTCACTCGAA GTAATTGAGA TCCAGGCCCG CTCCTCTCTG
    GGGACCCCCG CCCGAGCATA TTTGGAAATC CCCAACCCAC GCTACCTGGG CCCTGCAATC
    TCCTCAGGAG CCATCTACCT GGCGTCCTCA TATCAGGATA AGTTAAGGGT CATCTGCTGC
    AAAGGAAACC TGGTGAAGGA GACCGGCACC GACCACCACC GTGGCCCATC CACCTCCCGC
    AGCCCCAACA AGCGAGGCCC GCCGACGTAC AACGAGCACA TCACCAAGCG CGTGGCCTCA
    AGCCCGGCGC CTCCCGAAGG CCCCAGCCAC CCTCGAGAGC CAAGCACACC CCACCGCTAC
    CGCGAGGGGC GGACCGAGCT GCGCAGGGAC AAGTCTCCGG GCCGCCCCCT GGAGCGCGAG
    AAGTCCCCCG GCCGGATGCT GAGCACGCGG AGGGAGCGCT CCCCCGGGCG GCTGTTTGAG
    GACAGCAGCA GGGGCCGGCT GCCCGTGGGG GCCGTGAGGA CCCCGCTGTC CCAGGTCAAC
    AAGGTCTGGG ACCAGTCTTC AGTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa224625
    Clone ID Related Accession (Same CDS sequence) XM_024977737.1
    Accession Version XM_024977737.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6162bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product citron Rho-interacting kinase isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGTTGAAGT TCAAGTATGG AGCACGGAAT CTACTGGACG CTGGTACTGT TGAATCCATT 
    GCCAGCCGGG CCTCCAGGCT GAATCTTTTC TTCCAGGGGA AACCACCCTC CATGACTCAA
    CAGCAGATGT CTCTTCTTTC CCGAGAAGCG ATATTAGATG CCCTCTTTGT TCTCTTTAAA
    GAATGCAGTC AGCCAGCTCT GATGAAGATT AAGCACGTGA GCAACTTTGT CCAAAAGTAT
    TCCGATATCA TAGCTGAGTT GCAAGAGCTC CAGCCTTCAG TGAAGGACTT TGAAGTGAAA
    AGTGTCGTAG GCTGCGGTCA CTTTGCTGAA GTACAGGTGG TCAGAGAGAA AGCGACTGGG
    GATGTCTATG CCATGAAAGT CATGAAGAAG AAGGCCTTGT TGGCCCAGGA GCAGGTCTCA
    TATTTTGAGG AAGAACAGAA CATATTATCT CAGAGTACAA GTCCTTGGAT CCCCCAGTTG
    CAGTATGCCT TTCAGGACAA AAATAACCTT TATCTGGTCA TGGAATATCA GCCTGGAGGG
    GATTTGCTGT CACTTTTGAA TAGATATGAG GACCAATTAG ATGAAAATAT GATTCAGTTT
    TACCTAGCTG AACTGATTTT GGCTGTCCAC AGCGTTCATC AGATGGGCTA TGTACATCGA
    GACATCAAGC CCGAGAACAT CCTCATTGAC CGAACGGGAC ACATCAAGCT GGTGGATTTT
    GGATCAGCTG CTAAAATGAA CTCAAATAAG ATGGTAAATG CCAAACTCCC GGTTGGTACT
    CCAGATTACA TGGCCCCTGA AGTGTTGACT GTGATGAATG GGGATGGAAA AGGTGCCTAT
    AGCCTAGACT GTGATTGGTG GTCAGTGGGA GTTATTGCTT ATGAGATGGT TTATGGAAGA
    TGCCCATTCA CTGAAGGAAC CTCAGCCAAA ACCTTCAATA ACATCATGAA TTTCCAGCGG
    TTCTTGAAGT TTCCAGATGA CCCCAAAGTT AGCAGTGAAT TGCTTGATCT AATCCAGAGT
    TTGTTGTGTG GCCAGAAAGA GAGACTGAAG TTTGAGGGCC TTTGCTGTCA TCCTTTCTTC
    TCCAAAATTG ACTGGATTAA CATTCGTAAC TCTCCTCCCC CCTTCGTTCC CACCCTCAAG
    TCTGATGATG ACACCTCCAA TTTTGATGAA CCAGAGAAGA ATTCGTGGGT TTCATCCTCT
    CCGTGCCAGC TGAACCCCTC AGGTTTCTCG GGCGAAGAAC TGCCGTTTGT GGGGTTTTCG
    TATAGCAAGG CACTGGGAAT TCTTGGTAGA TCTGAGCCTG TTGTATCAAG TCTGGACTCC
    CCTGCCAAGA CTAGCTCCAT GGAAAAGAAA CTCCTTATCA AAAGCAAAGA GCTGCAGGAC
    TCCCAGGACA AGTGTCACAA GATGGAGCAG GAAATGACCC GGTTACATCG GAGAGTGTCA
    GAGGTGGAGG CTGTGCTTAG TCAGAAGGAG GTGGAGCTGA AGGCCTCTGA GACTCAGAGA
    TCCCTCCTGG AGCAGGACCT TGCTACCTAC ATCACAGAAT GCAGTAGCTT AAAGCGAAGT
    TTGGAGCAAG CACGGATGGA GGTGTCCCAA GAGGACGACA AAGCACTGCA GCTCCTCCAT
    GATATCAGAG AGCAGAGCCG GAAGCTCCAA GAAATCAAAG AGCAGGAGTA CCAGGCTCAA
    GTGGAAGAAA TGAGGTTGAT GATGAATCAG TTGGAAGAGG ACCTGGTTTC GGCGAGAAGG
    CGGAGTGACC TCTATGAATC GGAGCTCAGA GAGTCTCGGC TGGCTGCTGA AGAGTTTAAG
    CGGAAAGCAA CAGAATGTCA GCATAAACTG ATGAAGGCTA AAGATCAAGG GAAGCCTGAA
    GTGGGAGAAT ATGCCAAACT GGAGAAGATC AATGCTGAGC AGCAGCTCAA AATTCAGGAG
    CTCCAAGAGA AGCTAGAAAA GGCTGTGAAA GCTAGCACAG AGGCCACCGA GCTGCTGCAG
    AACATCCGTC AGGCGAAGGA GCGGGCCGAG CGTGAGCTGG AGAAGCTGCA CAACCGTGAG
    GACTCCTCCG AGGGCATCAA GAAGAAGCTG GTGGAGGCCG AGGAACGCCG CCACTCTCTG
    GAGAACAAGG TAAAGAGGCT AGAGACCATG GAGCGTAGAG AAAACAGACT GAAGGATGAC
    ATCCAGACAA AGTCCCAACA GATCCAGCAG ATGGCTGATA AAATTCTGGA GCTGGAGGAG
    AAGCACAGAG AAGCCCAGGT CTCAGCGCAG CATCTGGAGG TGCACCTGAA ACAGAAAGAG
    CAGCACTATG AAGAAAAAAT TAAAGTGTTG GACAATCAGA TAAAGAAAGA CCTAGCAGAC
    AAGGAGACAC TGGAGAACCT GATGCAAAGA CACGAAGAGG AGGCCCATGA GAAAGGCAAA
    ATTCTCAGCG AGCAGAAGGC GATGATCAAT GCTATGGATT CCAAGATCAG ATCCCTGGAA
    CAAAGGATAG TGGAATTGTC GGAAGCCAAT AAACTTGCGG CCAACAGCAG TCTCTTTACC
    CAGAGGAACA TGAAGGCCCA GGAAGAGATG ATTTCAGAAC TCAGGCAACA GAAGTTTTAC
    CTGGAGACGC AAGCTGGGAA ATTGGAGGCC CAGAACCGGA AGCTGGAGGA GCAGCTGGAA
    AAAATAAGCC ACCAAGACCA CAGTGACAAG AACCGTCTGC TGGAGCTGGA GACCAGGCTA
    AGGGAGGTCA GTCTAGAACA TGAAGAGCAG AAACTGGAGC TCAAACGCCA GCTCACAGAG
    CTGCAGCTCT CCCTGCAGGA GCGTGAATCC CAGCTGACAG CCCTGCAGGC AGCACGGGCG
    GCCCTGGAGA GCCAGCTCCG CCAGGCCAAG ACGGAGCTGG AAGAGACGAC AGCAGAAGCA
    GAAGAAGAGA TCCAGGCGCT TACGGCACAT AGAGATGAAA TCCAGCGCAA ATTCGATGCC
    CTTCGTAACA GCTGTACTGT GATCACGGAC TTGGAGGAGC AGCTAAACCA GCTGACGGAG
    GACAATGCCG AGCTCAACAA CCAAAACTTC TACTTGTCTA AACAACTCGA TGAGGCCTCT
    GGTGCCAACG ACGAGATTGT GCAGCTGCGA AGTGAAGTGG ACCATCTCCG CCGCGAGATC
    ACTGAGAGGG AGATGCAGCT CACTAGCCAG AAGCAAACGA TGGAGGCGCT GAAGACCACG
    TGCACAATGC TGGAGGAGCA GGTCATGGAC CTGGAGGCCC TGAACGACGA GCTGCTGGAG
    AAGGAGCGGC AGTGGGAGGC CTGGAGGAGT GTCCTTGGTG ACGAGAAGTC CCAGTTTGAG
    TGTCGGGTTC GAGAGTTGCA GAGGATGCTG GACACCGAAA AGCAGAGCAG GGCGAGGGCC
    GACCAGAGGA TCACTGAGTC GCGCCAGGTG GTCGAGCTGG CAGTAAAGGA GCACAAGGCC
    GAGATCCTGG CCTTGCAGCA GGCTCTCAAG GAGCAGAAGC TGAAGGCCGA GAGCCTCTCC
    GACAAGCTCA ATGATCTGGA GAAGAAGCAC GCCATGCTTG AAATGAATGC CCGAAGTTTA
    CAACAGAAGC TGGAGACAGA ACGAGAGCTC AAACAAAGGC TTCTGGAAGA GCAAGCCAAG
    TTACAGCAGC AGATGGACCT GCAGAAGAAT CATATTTTCC GTCTGACTCA AGGGCTGCAA
    GAAGCTCTGG ATCGGGCTGA TCTGCTGAAG ACAGAAAGGA GTGACCTGGA ATATCAGCTG
    GAAAACATTC AGGTTCTCTA TTCTCATGAG AAGGTGAAGA TGGAAGGCAC TATTTCCCAG
    CAAACCAAAC TCATCGATTT TCTGCAAGCC AAAATGGACC AGCCTGCCAA AAAGAAAAAG
    GTTCCTCTGC AGTACAATGA GCTGAAGGTG GCTCTGGAGA AGGAGAAAGC TCGCTGCGCG
    GAGCTCGAGG AAGCCCTGCA GAAGACCCGC ATCGAGCTCC GCTCCGCCCG GGAGGAAGCT
    GCCCACCGGA AAGCTGCAGA CCACCCACAC CCATCTACGC CGGCTACCGC CAGGCAGCAG
    ATCGCCATGT CCGCCATTGT GCGGTCACCC GAGCACCAGC CCAGCGCCCT GAGCCTGCTC
    GCCCCGCCGT CCAGCCGCAG GAAGGAGTCT TCCACTCCAG AGGAATTCAG CCGGCGTCTT
    AAGGAGCGCA TGCACCACAA CATCCCTCAC CGGTTCAACG TGGGACTGAA CATGCGAGCC
    ACGAAGTGCG CCGTGTGTCT GGATACCGTG CACTTCGGGC GCCAGGCATC CAAGTGTCTC
    GAATGTCAGG TCATGTGCCA CCCCAAGTGC TCCACATGCT TGCCAGCCAC CTGTGGCCTG
    CCAGCCGAAT ACGCCACACA CTTCACCGAG GCCTTCTGCC GCGACAAAAT GAACTCCCCG
    GGTCTGCAGT CCAAGGAGCC CAGCAGCAGC CTGCACCTGG AAGGGTGGAT GAAGGTGCCC
    AGGAATAACA AGCGAGGACA GCAAGGCTGG GACAGGAAGT ACATCGTTCT GGAGGGCTCC
    AAAGTCCTCA TTTATGACAA CGAAGCCAGA GAAGCTGGAC AGAGGCCGGT GGAAGAATTT
    GAGCTGTGCC TTCCCGACGG GGACGTGTCT ATTCATGGCG CCGTTGGTGC TTCTGAACTT
    GCAAACACAG CCAAAGCAGA TGTCCCCTAT GTCCTGAAGA TGGAGTCTCA CCCCCATACC
    ACCTGCTGGC CTGGGAGGAC CCTCTACTTG CTGGCTCCCA GCTTTCCCGA CAAGCAGCGC
    TGGGTCACCG CCTTAGAGTC AGTTGTGGCA GGTGGGAGAG TGTCTAGGGA AAAGGCGGAA
    GCCGACGCTA AATTGCTCGG AAACTCCCTG TTGAAACTGG AAGGTGATGA CCGTCTGGAC
    ATGAACTGCA CGCTGCCCTT CAGCGACCAG GTGGTGCTGG TGGGCACCGA GGAAGGGCTG
    TATGCGCTGA ATGTCTTGAA GAACTCCCTC ACCCACGTCC CAGGAATCGG AGCAGTCTTC
    CAGATTTACA TCATCAAGGA CCTGGAGAAG CTCCTCATGA TCGCAGGAGA GGAGCGGGCC
    CTGTGTCTCG TGGATGTGAA GAAGGTGAAG CAGTCCCTCG CGCAGTCTCA CCTCCCCGCC
    CAGCCAGACA TCTCGCCCAA CATCTTTGAA GCGGTCAAGG GCTGCCACTT GTTTGCTGCT
    GGCAAGATTG AGAGCGGGCT CTGCATCTGT GCAGCCATGC CCAGCAAAGT TGTCATTCTC
    CGCTACAATG AAAACCTCAG CAAGTACTGC ATTCGCAAAG AGATCGAGAC CTCGGAGCCC
    TGCAGCTGTA TCCACTTCAC CAACTACAGT ATCCTCATCG GAACCAATAA ATTCTACGAA
    ATCGACATGA AGCAGTACAC GCTAGAGGAA TTCCTGGATA AGAACGACCA TTCCTTGGCG
    CCTGCCGTGT TTGCCTCCTC TTCCAACAGC TTCCCCGTGT CCATCATGCA GGTGAACGGC
    GCGGGGCAGC GGGAGGAGTT CCTGCTCTGT TTCCACGAAT TCGGGGTGTT CGTGGATTCT
    TATGGAAGAC GCAGCCGCAC AGATGATCTC AAGTGGAGTC GCTTACCTCT GGCCTTTGCC
    TACAGAGAAC CCTATCTGTT TGTGACTCAC TTCAACTCAC TCGAAGTAAT TGAGATCCAG
    GCCCGCTCCT CTCTGGGGAC CCCCGCCCGA GCATATTTGG AAATCCCCAA CCCACGCTAC
    CTGGGCCCTG CAATCTCCTC AGGAGCCATC TACCTGGCGT CCTCATATCA GGATAAGTTA
    AGGGTCATCT GCTGCAAAGG AAACCTGGTG AAGGAGACCG GCACCGACCA CCACCGTGGC
    CCATCCACCT CCCGCAGCCC CAACAAGCGA GGCCCGCCGA CGTACAACGA GCACATCACC
    AAGCGCGTGG CCTCAAGCCC GGCGCCTCCC GAAGGCCCCA GCCACCCTCG AGAGCCAAGC
    ACACCCCACC GCTACCGCGA GGGGCGGACC GAGCTGCGCA GGGACAAGTC TCCGGGCCGC
    CCCCTGGAGC GCGAGAAGTC CCCCGGCCGG ATGCTGAGCA CGCGGAGGGA GCGCTCCCCC
    GGGCGGCTGT TTGAGGACAG CAGCAGGGGC CGGCTGCCCG TGGGGGCCGT GAGGACCCCG
    CTGTCCCAGG TCAACAAGGT CTGGGACCAG TCTTCAGTAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024833505.1
    CDS113..6274
    Translation

    Target ORF information:

    RefSeq Version XM_024977737.1
    Organism Bos taurus(cattle)
    Definition Bos taurus citron rho-interacting serine/threonine kinase (CIT), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024977737.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGTTGAAGT TCAAGTATGG AGCACGGAAT CTACTGGACG CTGGTACTGT TGAATCCATT 
    GCCAGCCGGG CCTCCAGGCT GAATCTTTTC TTCCAGGGGA AACCACCCTC CATGACTCAA
    CAGCAGATGT CTCTTCTTTC CCGAGAAGCG ATATTAGATG CCCTCTTTGT TCTCTTTAAA
    GAATGCAGTC AGCCAGCTCT GATGAAGATT AAGCACGTGA GCAACTTTGT CCAAAAGTAT
    TCCGATATCA TAGCTGAGTT GCAAGAGCTC CAGCCTTCAG TGAAGGACTT TGAAGTGAAA
    AGTGTCGTAG GCTGCGGTCA CTTTGCTGAA GTACAGGTGG TCAGAGAGAA AGCGACTGGG
    GATGTCTATG CCATGAAAGT CATGAAGAAG AAGGCCTTGT TGGCCCAGGA GCAGGTCTCA
    TATTTTGAGG AAGAACAGAA CATATTATCT CAGAGTACAA GTCCTTGGAT CCCCCAGTTG
    CAGTATGCCT TTCAGGACAA AAATAACCTT TATCTGGTCA TGGAATATCA GCCTGGAGGG
    GATTTGCTGT CACTTTTGAA TAGATATGAG GACCAATTAG ATGAAAATAT GATTCAGTTT
    TACCTAGCTG AACTGATTTT GGCTGTCCAC AGCGTTCATC AGATGGGCTA TGTACATCGA
    GACATCAAGC CCGAGAACAT CCTCATTGAC CGAACGGGAC ACATCAAGCT GGTGGATTTT
    GGATCAGCTG CTAAAATGAA CTCAAATAAG ATGGTAAATG CCAAACTCCC GGTTGGTACT
    CCAGATTACA TGGCCCCTGA AGTGTTGACT GTGATGAATG GGGATGGAAA AGGTGCCTAT
    AGCCTAGACT GTGATTGGTG GTCAGTGGGA GTTATTGCTT ATGAGATGGT TTATGGAAGA
    TGCCCATTCA CTGAAGGAAC CTCAGCCAAA ACCTTCAATA ACATCATGAA TTTCCAGCGG
    TTCTTGAAGT TTCCAGATGA CCCCAAAGTT AGCAGTGAAT TGCTTGATCT AATCCAGAGT
    TTGTTGTGTG GCCAGAAAGA GAGACTGAAG TTTGAGGGCC TTTGCTGTCA TCCTTTCTTC
    TCCAAAATTG ACTGGATTAA CATTCGTAAC TCTCCTCCCC CCTTCGTTCC CACCCTCAAG
    TCTGATGATG ACACCTCCAA TTTTGATGAA CCAGAGAAGA ATTCGTGGGT TTCATCCTCT
    CCGTGCCAGC TGAACCCCTC AGGTTTCTCG GGCGAAGAAC TGCCGTTTGT GGGGTTTTCG
    TATAGCAAGG CACTGGGAAT TCTTGGTAGA TCTGAGCCTG TTGTATCAAG TCTGGACTCC
    CCTGCCAAGA CTAGCTCCAT GGAAAAGAAA CTCCTTATCA AAAGCAAAGA GCTGCAGGAC
    TCCCAGGACA AGTGTCACAA GATGGAGCAG GAAATGACCC GGTTACATCG GAGAGTGTCA
    GAGGTGGAGG CTGTGCTTAG TCAGAAGGAG GTGGAGCTGA AGGCCTCTGA GACTCAGAGA
    TCCCTCCTGG AGCAGGACCT TGCTACCTAC ATCACAGAAT GCAGTAGCTT AAAGCGAAGT
    TTGGAGCAAG CACGGATGGA GGTGTCCCAA GAGGACGACA AAGCACTGCA GCTCCTCCAT
    GATATCAGAG AGCAGAGCCG GAAGCTCCAA GAAATCAAAG AGCAGGAGTA CCAGGCTCAA
    GTGGAAGAAA TGAGGTTGAT GATGAATCAG TTGGAAGAGG ACCTGGTTTC GGCGAGAAGG
    CGGAGTGACC TCTATGAATC GGAGCTCAGA GAGTCTCGGC TGGCTGCTGA AGAGTTTAAG
    CGGAAAGCAA CAGAATGTCA GCATAAACTG ATGAAGGCTA AAGATCAAGG GAAGCCTGAA
    GTGGGAGAAT ATGCCAAACT GGAGAAGATC AATGCTGAGC AGCAGCTCAA AATTCAGGAG
    CTCCAAGAGA AGCTAGAAAA GGCTGTGAAA GCTAGCACAG AGGCCACCGA GCTGCTGCAG
    AACATCCGTC AGGCGAAGGA GCGGGCCGAG CGTGAGCTGG AGAAGCTGCA CAACCGTGAG
    GACTCCTCCG AGGGCATCAA GAAGAAGCTG GTGGAGGCCG AGGAACGCCG CCACTCTCTG
    GAGAACAAGG TAAAGAGGCT AGAGACCATG GAGCGTAGAG AAAACAGACT GAAGGATGAC
    ATCCAGACAA AGTCCCAACA GATCCAGCAG ATGGCTGATA AAATTCTGGA GCTGGAGGAG
    AAGCACAGAG AAGCCCAGGT CTCAGCGCAG CATCTGGAGG TGCACCTGAA ACAGAAAGAG
    CAGCACTATG AAGAAAAAAT TAAAGTGTTG GACAATCAGA TAAAGAAAGA CCTAGCAGAC
    AAGGAGACAC TGGAGAACCT GATGCAAAGA CACGAAGAGG AGGCCCATGA GAAAGGCAAA
    ATTCTCAGCG AGCAGAAGGC GATGATCAAT GCTATGGATT CCAAGATCAG ATCCCTGGAA
    CAAAGGATAG TGGAATTGTC GGAAGCCAAT AAACTTGCGG CCAACAGCAG TCTCTTTACC
    CAGAGGAACA TGAAGGCCCA GGAAGAGATG ATTTCAGAAC TCAGGCAACA GAAGTTTTAC
    CTGGAGACGC AAGCTGGGAA ATTGGAGGCC CAGAACCGGA AGCTGGAGGA GCAGCTGGAA
    AAAATAAGCC ACCAAGACCA CAGTGACAAG AACCGTCTGC TGGAGCTGGA GACCAGGCTA
    AGGGAGGTCA GTCTAGAACA TGAAGAGCAG AAACTGGAGC TCAAACGCCA GCTCACAGAG
    CTGCAGCTCT CCCTGCAGGA GCGTGAATCC CAGCTGACAG CCCTGCAGGC AGCACGGGCG
    GCCCTGGAGA GCCAGCTCCG CCAGGCCAAG ACGGAGCTGG AAGAGACGAC AGCAGAAGCA
    GAAGAAGAGA TCCAGGCGCT TACGGCACAT AGAGATGAAA TCCAGCGCAA ATTCGATGCC
    CTTCGTAACA GCTGTACTGT GATCACGGAC TTGGAGGAGC AGCTAAACCA GCTGACGGAG
    GACAATGCCG AGCTCAACAA CCAAAACTTC TACTTGTCTA AACAACTCGA TGAGGCCTCT
    GGTGCCAACG ACGAGATTGT GCAGCTGCGA AGTGAAGTGG ACCATCTCCG CCGCGAGATC
    ACTGAGAGGG AGATGCAGCT CACTAGCCAG AAGCAAACGA TGGAGGCGCT GAAGACCACG
    TGCACAATGC TGGAGGAGCA GGTCATGGAC CTGGAGGCCC TGAACGACGA GCTGCTGGAG
    AAGGAGCGGC AGTGGGAGGC CTGGAGGAGT GTCCTTGGTG ACGAGAAGTC CCAGTTTGAG
    TGTCGGGTTC GAGAGTTGCA GAGGATGCTG GACACCGAAA AGCAGAGCAG GGCGAGGGCC
    GACCAGAGGA TCACTGAGTC GCGCCAGGTG GTCGAGCTGG CAGTAAAGGA GCACAAGGCC
    GAGATCCTGG CCTTGCAGCA GGCTCTCAAG GAGCAGAAGC TGAAGGCCGA GAGCCTCTCC
    GACAAGCTCA ATGATCTGGA GAAGAAGCAC GCCATGCTTG AAATGAATGC CCGAAGTTTA
    CAACAGAAGC TGGAGACAGA ACGAGAGCTC AAACAAAGGC TTCTGGAAGA GCAAGCCAAG
    TTACAGCAGC AGATGGACCT GCAGAAGAAT CATATTTTCC GTCTGACTCA AGGGCTGCAA
    GAAGCTCTGG ATCGGGCTGA TCTGCTGAAG ACAGAAAGGA GTGACCTGGA ATATCAGCTG
    GAAAACATTC AGGTTCTCTA TTCTCATGAG AAGGTGAAGA TGGAAGGCAC TATTTCCCAG
    CAAACCAAAC TCATCGATTT TCTGCAAGCC AAAATGGACC AGCCTGCCAA AAAGAAAAAG
    GTTCCTCTGC AGTACAATGA GCTGAAGGTG GCTCTGGAGA AGGAGAAAGC TCGCTGCGCG
    GAGCTCGAGG AAGCCCTGCA GAAGACCCGC ATCGAGCTCC GCTCCGCCCG GGAGGAAGCT
    GCCCACCGGA AAGCTGCAGA CCACCCACAC CCATCTACGC CGGCTACCGC CAGGCAGCAG
    ATCGCCATGT CCGCCATTGT GCGGTCACCC GAGCACCAGC CCAGCGCCCT GAGCCTGCTC
    GCCCCGCCGT CCAGCCGCAG GAAGGAGTCT TCCACTCCAG AGGAATTCAG CCGGCGTCTT
    AAGGAGCGCA TGCACCACAA CATCCCTCAC CGGTTCAACG TGGGACTGAA CATGCGAGCC
    ACGAAGTGCG CCGTGTGTCT GGATACCGTG CACTTCGGGC GCCAGGCATC CAAGTGTCTC
    GAATGTCAGG TCATGTGCCA CCCCAAGTGC TCCACATGCT TGCCAGCCAC CTGTGGCCTG
    CCAGCCGAAT ACGCCACACA CTTCACCGAG GCCTTCTGCC GCGACAAAAT GAACTCCCCG
    GGTCTGCAGT CCAAGGAGCC CAGCAGCAGC CTGCACCTGG AAGGGTGGAT GAAGGTGCCC
    AGGAATAACA AGCGAGGACA GCAAGGCTGG GACAGGAAGT ACATCGTTCT GGAGGGCTCC
    AAAGTCCTCA TTTATGACAA CGAAGCCAGA GAAGCTGGAC AGAGGCCGGT GGAAGAATTT
    GAGCTGTGCC TTCCCGACGG GGACGTGTCT ATTCATGGCG CCGTTGGTGC TTCTGAACTT
    GCAAACACAG CCAAAGCAGA TGTCCCCTAT GTCCTGAAGA TGGAGTCTCA CCCCCATACC
    ACCTGCTGGC CTGGGAGGAC CCTCTACTTG CTGGCTCCCA GCTTTCCCGA CAAGCAGCGC
    TGGGTCACCG CCTTAGAGTC AGTTGTGGCA GGTGGGAGAG TGTCTAGGGA AAAGGCGGAA
    GCCGACGCTA AATTGCTCGG AAACTCCCTG TTGAAACTGG AAGGTGATGA CCGTCTGGAC
    ATGAACTGCA CGCTGCCCTT CAGCGACCAG GTGGTGCTGG TGGGCACCGA GGAAGGGCTG
    TATGCGCTGA ATGTCTTGAA GAACTCCCTC ACCCACGTCC CAGGAATCGG AGCAGTCTTC
    CAGATTTACA TCATCAAGGA CCTGGAGAAG CTCCTCATGA TCGCAGGAGA GGAGCGGGCC
    CTGTGTCTCG TGGATGTGAA GAAGGTGAAG CAGTCCCTCG CGCAGTCTCA CCTCCCCGCC
    CAGCCAGACA TCTCGCCCAA CATCTTTGAA GCGGTCAAGG GCTGCCACTT GTTTGCTGCT
    GGCAAGATTG AGAGCGGGCT CTGCATCTGT GCAGCCATGC CCAGCAAAGT TGTCATTCTC
    CGCTACAATG AAAACCTCAG CAAGTACTGC ATTCGCAAAG AGATCGAGAC CTCGGAGCCC
    TGCAGCTGTA TCCACTTCAC CAACTACAGT ATCCTCATCG GAACCAATAA ATTCTACGAA
    ATCGACATGA AGCAGTACAC GCTAGAGGAA TTCCTGGATA AGAACGACCA TTCCTTGGCG
    CCTGCCGTGT TTGCCTCCTC TTCCAACAGC TTCCCCGTGT CCATCATGCA GGTGAACGGC
    GCGGGGCAGC GGGAGGAGTT CCTGCTCTGT TTCCACGAAT TCGGGGTGTT CGTGGATTCT
    TATGGAAGAC GCAGCCGCAC AGATGATCTC AAGTGGAGTC GCTTACCTCT GGCCTTTGCC
    TACAGAGAAC CCTATCTGTT TGTGACTCAC TTCAACTCAC TCGAAGTAAT TGAGATCCAG
    GCCCGCTCCT CTCTGGGGAC CCCCGCCCGA GCATATTTGG AAATCCCCAA CCCACGCTAC
    CTGGGCCCTG CAATCTCCTC AGGAGCCATC TACCTGGCGT CCTCATATCA GGATAAGTTA
    AGGGTCATCT GCTGCAAAGG AAACCTGGTG AAGGAGACCG GCACCGACCA CCACCGTGGC
    CCATCCACCT CCCGCAGCCC CAACAAGCGA GGCCCGCCGA CGTACAACGA GCACATCACC
    AAGCGCGTGG CCTCAAGCCC GGCGCCTCCC GAAGGCCCCA GCCACCCTCG AGAGCCAAGC
    ACACCCCACC GCTACCGCGA GGGGCGGACC GAGCTGCGCA GGGACAAGTC TCCGGGCCGC
    CCCCTGGAGC GCGAGAAGTC CCCCGGCCGG ATGCTGAGCA CGCGGAGGGA GCGCTCCCCC
    GGGCGGCTGT TTGAGGACAG CAGCAGGGGC CGGCTGCCCG TGGGGGCCGT GAGGACCCCG
    CTGTCCCAGG TCAACAAGGT CTGGGACCAG TCTTCAGTAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa224626
    Clone ID Related Accession (Same CDS sequence) XM_024977738.1
    Accession Version XM_024977738.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6054bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product citron Rho-interacting kinase isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    ATGTTGAAGT TCAAGTATGG AGCACGGAAT CTACTGGACG CTGGTACTGT TGAATCCATT 
    GCCAGCCGGG CCTCCAGGCT GAATCTTTTC TTCCAGGGGA AACCACCCTC CATGACTCAA
    CAGCAGATGT CTCTTCTTTC CCGAGAAGCG ATATTAGATG CCCTCTTTGT TCTCTTTAAA
    GAATGCAGTC AGCCAGCTCT GATGAAGATT AAGCACGTGA GCAACTTTGT CCAAAAGTAT
    TCCGATATCA TAGCTGAGTT GCAAGAGCTC CAGCCTTCAG TGAAGGACTT TGAAGTGAAA
    AGTGTCGTAG GCTGCGGTCA CTTTGCTGAA GTACAGGTGG TCAGAGAGAA AGCGACTGGG
    GATGTCTATG CCATGAAAGT CATGAAGAAG AAGGCCTTGT TGGCCCAGGA GCAGGTCTCA
    TATTTTGAGG AAGAACAGAA CATATTATCT CAGAGTACAA GTCCTTGGAT CCCCCAGTTG
    CAGTATGCCT TTCAGGACAA AAATAACCTT TATCTGGTCA TGGAATATCA GCCTGGAGGG
    GATTTGCTGT CACTTTTGAA TAGATATGAG GACCAATTAG ATGAAAATAT GATTCAGTTT
    TACCTAGCTG AACTGATTTT GGCTGTCCAC AGCGTTCATC AGATGGGCTA TGTACATCGA
    GACATCAAGC CCGAGAACAT CCTCATTGAC CGAACGGGAC ACATCAAGCT GGTGGATTTT
    GGATCAGCTG CTAAAATGAA CTCAAATAAG ATGGTAAATG CCAAACTCCC GGTTGGTACT
    CCAGATTACA TGGCCCCTGA AGTGTTGACT GTGATGAATG GGGATGGAAA AGGTGCCTAT
    AGCCTAGACT GTGATTGGTG GTCAGTGGGA GTTATTGCTT ATGAGATGGT TTATGGAAGA
    TGCCCATTCA CTGAAGGAAC CTCAGCCAAA ACCTTCAATA ACATCATGAA TTTCCAGCGG
    TTCTTGAAGT TTCCAGATGA CCCCAAAGTT AGCAGTGAAT TGCTTGATCT AATCCAGAGT
    TTGTTGTGTG GCCAGAAAGA GAGACTGAAG TTTGAGGGCC TTTGCTGTCA TCCTTTCTTC
    TCCAAAATTG ACTGGATTAA CATTCGTAAC TCTCCTCCCC CCTTCGTTCC CACCCTCAAG
    TCTGATGATG ACACCTCCAA TTTTGATGAA CCAGAGAAGA ATTCGTGGGT TTCATCCTCT
    CCGTGCCAGC TGAACCCCTC AGGTTTCTCG GGCGAAGAAC TGCCGTTTGT GGGGTTTTCG
    TATAGCAAGG CACTGGGAAT TCTTGGTAGA TCTGAGCCTG TTGTATCAAG TCTGGACTCC
    CCTGCCAAGA CTAGCTCCAT GGAAAAGAAA CTCCTTATCA AAAGCAAAGA GCTGCAGGAC
    TCCCAGGACA AGTGTCACAA GATGGAGCAG GAAATGACCC GGTTACATCG GAGAGTGTCA
    GAGGTGGAGG CTGTGCTTAG TCAGAAGGAG GTGGAGCTGA AGGCCTCTGA GACTCAGAGA
    TCCCTCCTGG AGCAGGACCT TGCTACCTAC ATCACAGAAT GCAGTAGCTT AAAGCGAAGT
    TTGGAGCAAG CACGGATGGA GGTGTCCCAA GAGGACGACA AAGCACTGCA GCTCCTCCAT
    GATATCAGAG AGCAGAGCCG GAAGCTCCAA GAAATCAAAG AGCAGGAGTA CCAGGCTCAA
    GTGGAAGAAA TGAGGTTGAT GATGAATCAG TTGGAAGAGG ACCTGGTTTC GGCGAGAAGG
    CGGAGTGACC TCTATGAATC GGAGCTCAGA GAGTCTCGGC TGGCTGCTGA AGAGTTTAAG
    CGGAAAGCAA CAGAATGTCA GCATAAACTG ATGAAGGCTA AAGATCAAGG GAAGCCTGAA
    GTGGGAGAAT ATGCCAAACT GGAGAAGATC AATGCTGAGC AGCAGCTCAA AATTCAGGAG
    CTCCAAGAGA AGCTAGAAAA GGCTGTGAAA GCTAGCACAG AGGCCACCGA GCTGCTGCAG
    AACATCCGTC AGGCGAAGGA GCGGGCCGAG CGTGAGCTGG AGAAGCTGCA CAACCGTGAG
    GACTCCTCCG AGGGCATCAA GAAGAAGCTG GTGGAGGCCG AGGAACGCCG CCACTCTCTG
    GAGAACAAGG TAAAGAGGCT AGAGACCATG GAGCGTAGAG AAAACAGACT GAAGGATGAC
    ATCCAGACAA AGTCCCAACA GATCCAGCAG ATGGCTGATA AAATTCTGGA GCTGGAGGAG
    AAGCACAGAG AAGCCCAGGT CTCAGCGCAG CATCTGGAGG TGCACCTGAA ACAGAAAGAG
    CAGCACTATG AAGAAAAAAT TAAAGTGTTG GACAATCAGA TAAAGAAAGA CCTAGCAGAC
    AAGGAGACAC TGGAGAACCT GATGCAAAGA CACGAAGAGG AGGCCCATGA GAAAGGCAAA
    ATTCTCAGCG AGCAGAAGGC GATGATCAAT GCTATGGATT CCAAGATCAG ATCCCTGGAA
    CAAAGGATAG TGGAATTGTC GGAAGCCAAT AAACTTGCGG CCAACAGCAG TCTCTTTACC
    CAGAGGAACA TGAAGGCCCA GGAAGAGATG ATTTCAGAAC TCAGGCAACA GAAGTTTTAC
    CTGGAGACGC AAGCTGGGAA ATTGGAGGCC CAGAACCGGA AGCTGGAGGA GCAGCTGGAA
    AAAATAAGCC ACCAAGACCA CAGTGACAAG AACCGTCTGC TGGAGCTGGA GACCAGGCTA
    AGGGAGGCAC ATAGAGATGA AATCCAGCGC AAATTCGATG CCCTTCGTAA CAGCTGTACT
    GTGATCACGG ACTTGGAGGA GCAGCTAAAC CAGCTGACGG AGGACAATGC CGAGCTCAAC
    AACCAAAACT TCTACTTGTC TAAACAACTC GATGAGGCCT CTGGTGCCAA CGACGAGATT
    GTGCAGCTGC GAAGTGAAGT GGACCATCTC CGCCGCGAGA TCACTGAGAG GGAGATGCAG
    CTCACTAGCC AGAAGCAAAC GATGGAGGCG CTGAAGACCA CGTGCACAAT GCTGGAGGAG
    CAGGTCATGG ACCTGGAGGC CCTGAACGAC GAGCTGCTGG AGAAGGAGCG GCAGTGGGAG
    GCCTGGAGGA GTGTCCTTGG TGACGAGAAG TCCCAGTTTG AGTGTCGGGT TCGAGAGTTG
    CAGAGGATGC TGGACACCGA AAAGCAGAGC AGGGCGAGGG CCGACCAGAG GATCACTGAG
    TCGCGCCAGG TGGTCGAGCT GGCAGTAAAG GAGCACAAGG CCGAGATCCT GGCCTTGCAG
    CAGGCTCTCA AGGAGCAGAA GCTGAAGGCC GAGAGCCTCT CCGACAAGCT CAATGATCTG
    GAGAAGAAGC ACGCCATGCT TGAAATGAAT GCCCGAAGTT TACAACAGAA GCTGGAGACA
    GAACGAGAGC TCAAACAAAG GCTTCTGGAA GAGCAAGCCA AGTTACAGCA GCAGATGGAC
    CTGCAGAAGA ATCATATTTT CCGTCTGACT CAAGGGCTGC AAGAAGCTCT GGATCGGGCT
    GATCTGCTGA AGACAGAAAG GAGTGACCTG GAATATCAGC TGGAAAACAT TCAGGTTCTC
    TATTCTCATG AGAAGGTGAA GATGGAAGGC ACTATTTCCC AGCAAACCAA ACTCATCGAT
    TTTCTGCAAG CCAAAATGGA CCAGCCTGCC AAAAAGAAAA AGGGTTTATT TAGTCGACGG
    AAAGAGGACC CTGCTTTGCC CACACAGGTT CCTCTGCAGT ACAATGAGCT GAAGGTGGCT
    CTGGAGAAGG AGAAAGCTCG CTGCGCGGAG CTCGAGGAAG CCCTGCAGAA GACCCGCATC
    GAGCTCCGCT CCGCCCGGGA GGAAGCTGCC CACCGGAAAG CTGCAGACCA CCCACACCCA
    TCTACGCCGG CTACCGCCAG GCAGCAGATC GCCATGTCCG CCATTGTGCG GTCACCCGAG
    CACCAGCCCA GCGCCCTGAG CCTGCTCGCC CCGCCGTCCA GCCGCAGGAA GGAGTCTTCC
    ACTCCAGAGG AATTCAGCCG GCGTCTTAAG GAGCGCATGC ACCACAACAT CCCTCACCGG
    TTCAACGTGG GACTGAACAT GCGAGCCACG AAGTGCGCCG TGTGTCTGGA TACCGTGCAC
    TTCGGGCGCC AGGCATCCAA GTGTCTCGAA TGTCAGGTCA TGTGCCACCC CAAGTGCTCC
    ACATGCTTGC CAGCCACCTG TGGCCTGCCA GCCGAATACG CCACACACTT CACCGAGGCC
    TTCTGCCGCG ACAAAATGAA CTCCCCGGGT CTGCAGTCCA AGGAGCCCAG CAGCAGCCTG
    CACCTGGAAG GGTGGATGAA GGTGCCCAGG AATAACAAGC GAGGACAGCA AGGCTGGGAC
    AGGAAGTACA TCGTTCTGGA GGGCTCCAAA GTCCTCATTT ATGACAACGA AGCCAGAGAA
    GCTGGACAGA GGCCGGTGGA AGAATTTGAG CTGTGCCTTC CCGACGGGGA CGTGTCTATT
    CATGGCGCCG TTGGTGCTTC TGAACTTGCA AACACAGCCA AAGCAGATGT CCCCTATGTC
    CTGAAGATGG AGTCTCACCC CCATACCACC TGCTGGCCTG GGAGGACCCT CTACTTGCTG
    GCTCCCAGCT TTCCCGACAA GCAGCGCTGG GTCACCGCCT TAGAGTCAGT TGTGGCAGGT
    GGGAGAGTGT CTAGGGAAAA GGCGGAAGCC GACGCTGGCC GTGACTGTAC TTCCTACGAG
    CCTCTGCCCG CGTGGGCTCA GAAATTGCTC GGAAACTCCC TGTTGAAACT GGAAGGTGAT
    GACCGTCTGG ACATGAACTG CACGCTGCCC TTCAGCGACC AGGTGGTGCT GGTGGGCACC
    GAGGAAGGGC TGTATGCGCT GAATGTCTTG AAGAACTCCC TCACCCACGT CCCAGGAATC
    GGAGCAGTCT TCCAGATTTA CATCATCAAG GACCTGGAGA AGCTCCTCAT GATCGCAGGA
    GAGGAGCGGG CCCTGTGTCT CGTGGATGTG AAGAAGGTGA AGCAGTCCCT CGCGCAGTCT
    CACCTCCCCG CCCAGCCAGA CATCTCGCCC AACATCTTTG AAGCGGTCAA GGGCTGCCAC
    TTGTTTGCTG CTGGCAAGAT TGAGAGCGGG CTCTGCATCT GTGCAGCCAT GCCCAGCAAA
    GTTGTCATTC TCCGCTACAA TGAAAACCTC AGCAAGTACT GCATTCGCAA AGAGATCGAG
    ACCTCGGAGC CCTGCAGCTG TATCCACTTC ACCAACTACA GTATCCTCAT CGGAACCAAT
    AAATTCTACG AAATCGACAT GAAGCAGTAC ACGCTAGAGG AATTCCTGGA TAAGAACGAC
    CATTCCTTGG CGCCTGCCGT GTTTGCCTCC TCTTCCAACA GCTTCCCCGT GTCCATCATG
    CAGGTGAACG GCGCGGGGCA GCGGGAGGAG TTCCTGCTCT GTTTCCACGA ATTCGGGGTG
    TTCGTGGATT CTTATGGAAG ACGCAGCCGC ACAGATGATC TCAAGTGGAG TCGCTTACCT
    CTGGCCTTTG CCTACAGAGA ACCCTATCTG TTTGTGACTC ACTTCAACTC ACTCGAAGTA
    ATTGAGATCC AGGCCCGCTC CTCTCTGGGG ACCCCCGCCC GAGCATATTT GGAAATCCCC
    AACCCACGCT ACCTGGGCCC TGCAATCTCC TCAGGAGCCA TCTACCTGGC GTCCTCATAT
    CAGGATAAGT TAAGGGTCAT CTGCTGCAAA GGAAACCTGG TGAAGGAGAC CGGCACCGAC
    CACCACCGTG GCCCATCCAC CTCCCGCAGC CCCAACAAGC GAGGCCCGCC GACGTACAAC
    GAGCACATCA CCAAGCGCGT GGCCTCAAGC CCGGCGCCTC CCGAAGGCCC CAGCCACCCT
    CGAGAGCCAA GCACACCCCA CCGCTACCGC GAGGGGCGGA CCGAGCTGCG CAGGGACAAG
    TCTCCGGGCC GCCCCCTGGA GCGCGAGAAG TCCCCCGGCC GGATGCTGAG CACGCGGAGG
    GAGCGCTCCC CCGGGCGGCT GTTTGAGGAC AGCAGCAGGG GCCGGCTGCC CGTGGGGGCC
    GTGAGGACCC CGCTGTCCCA GGTCAACAAG GTCTGGGACC AGTCTTCAGT ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024833506.1
    CDS113..6166
    Translation

    Target ORF information:

    RefSeq Version XM_024977738.1
    Organism Bos taurus(cattle)
    Definition Bos taurus citron rho-interacting serine/threonine kinase (CIT), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024977738.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    ATGTTGAAGT TCAAGTATGG AGCACGGAAT CTACTGGACG CTGGTACTGT TGAATCCATT 
    GCCAGCCGGG CCTCCAGGCT GAATCTTTTC TTCCAGGGGA AACCACCCTC CATGACTCAA
    CAGCAGATGT CTCTTCTTTC CCGAGAAGCG ATATTAGATG CCCTCTTTGT TCTCTTTAAA
    GAATGCAGTC AGCCAGCTCT GATGAAGATT AAGCACGTGA GCAACTTTGT CCAAAAGTAT
    TCCGATATCA TAGCTGAGTT GCAAGAGCTC CAGCCTTCAG TGAAGGACTT TGAAGTGAAA
    AGTGTCGTAG GCTGCGGTCA CTTTGCTGAA GTACAGGTGG TCAGAGAGAA AGCGACTGGG
    GATGTCTATG CCATGAAAGT CATGAAGAAG AAGGCCTTGT TGGCCCAGGA GCAGGTCTCA
    TATTTTGAGG AAGAACAGAA CATATTATCT CAGAGTACAA GTCCTTGGAT CCCCCAGTTG
    CAGTATGCCT TTCAGGACAA AAATAACCTT TATCTGGTCA TGGAATATCA GCCTGGAGGG
    GATTTGCTGT CACTTTTGAA TAGATATGAG GACCAATTAG ATGAAAATAT GATTCAGTTT
    TACCTAGCTG AACTGATTTT GGCTGTCCAC AGCGTTCATC AGATGGGCTA TGTACATCGA
    GACATCAAGC CCGAGAACAT CCTCATTGAC CGAACGGGAC ACATCAAGCT GGTGGATTTT
    GGATCAGCTG CTAAAATGAA CTCAAATAAG ATGGTAAATG CCAAACTCCC GGTTGGTACT
    CCAGATTACA TGGCCCCTGA AGTGTTGACT GTGATGAATG GGGATGGAAA AGGTGCCTAT
    AGCCTAGACT GTGATTGGTG GTCAGTGGGA GTTATTGCTT ATGAGATGGT TTATGGAAGA
    TGCCCATTCA CTGAAGGAAC CTCAGCCAAA ACCTTCAATA ACATCATGAA TTTCCAGCGG
    TTCTTGAAGT TTCCAGATGA CCCCAAAGTT AGCAGTGAAT TGCTTGATCT AATCCAGAGT
    TTGTTGTGTG GCCAGAAAGA GAGACTGAAG TTTGAGGGCC TTTGCTGTCA TCCTTTCTTC
    TCCAAAATTG ACTGGATTAA CATTCGTAAC TCTCCTCCCC CCTTCGTTCC CACCCTCAAG
    TCTGATGATG ACACCTCCAA TTTTGATGAA CCAGAGAAGA ATTCGTGGGT TTCATCCTCT
    CCGTGCCAGC TGAACCCCTC AGGTTTCTCG GGCGAAGAAC TGCCGTTTGT GGGGTTTTCG
    TATAGCAAGG CACTGGGAAT TCTTGGTAGA TCTGAGCCTG TTGTATCAAG TCTGGACTCC
    CCTGCCAAGA CTAGCTCCAT GGAAAAGAAA CTCCTTATCA AAAGCAAAGA GCTGCAGGAC
    TCCCAGGACA AGTGTCACAA GATGGAGCAG GAAATGACCC GGTTACATCG GAGAGTGTCA
    GAGGTGGAGG CTGTGCTTAG TCAGAAGGAG GTGGAGCTGA AGGCCTCTGA GACTCAGAGA
    TCCCTCCTGG AGCAGGACCT TGCTACCTAC ATCACAGAAT GCAGTAGCTT AAAGCGAAGT
    TTGGAGCAAG CACGGATGGA GGTGTCCCAA GAGGACGACA AAGCACTGCA GCTCCTCCAT
    GATATCAGAG AGCAGAGCCG GAAGCTCCAA GAAATCAAAG AGCAGGAGTA CCAGGCTCAA
    GTGGAAGAAA TGAGGTTGAT GATGAATCAG TTGGAAGAGG ACCTGGTTTC GGCGAGAAGG
    CGGAGTGACC TCTATGAATC GGAGCTCAGA GAGTCTCGGC TGGCTGCTGA AGAGTTTAAG
    CGGAAAGCAA CAGAATGTCA GCATAAACTG ATGAAGGCTA AAGATCAAGG GAAGCCTGAA
    GTGGGAGAAT ATGCCAAACT GGAGAAGATC AATGCTGAGC AGCAGCTCAA AATTCAGGAG
    CTCCAAGAGA AGCTAGAAAA GGCTGTGAAA GCTAGCACAG AGGCCACCGA GCTGCTGCAG
    AACATCCGTC AGGCGAAGGA GCGGGCCGAG CGTGAGCTGG AGAAGCTGCA CAACCGTGAG
    GACTCCTCCG AGGGCATCAA GAAGAAGCTG GTGGAGGCCG AGGAACGCCG CCACTCTCTG
    GAGAACAAGG TAAAGAGGCT AGAGACCATG GAGCGTAGAG AAAACAGACT GAAGGATGAC
    ATCCAGACAA AGTCCCAACA GATCCAGCAG ATGGCTGATA AAATTCTGGA GCTGGAGGAG
    AAGCACAGAG AAGCCCAGGT CTCAGCGCAG CATCTGGAGG TGCACCTGAA ACAGAAAGAG
    CAGCACTATG AAGAAAAAAT TAAAGTGTTG GACAATCAGA TAAAGAAAGA CCTAGCAGAC
    AAGGAGACAC TGGAGAACCT GATGCAAAGA CACGAAGAGG AGGCCCATGA GAAAGGCAAA
    ATTCTCAGCG AGCAGAAGGC GATGATCAAT GCTATGGATT CCAAGATCAG ATCCCTGGAA
    CAAAGGATAG TGGAATTGTC GGAAGCCAAT AAACTTGCGG CCAACAGCAG TCTCTTTACC
    CAGAGGAACA TGAAGGCCCA GGAAGAGATG ATTTCAGAAC TCAGGCAACA GAAGTTTTAC
    CTGGAGACGC AAGCTGGGAA ATTGGAGGCC CAGAACCGGA AGCTGGAGGA GCAGCTGGAA
    AAAATAAGCC ACCAAGACCA CAGTGACAAG AACCGTCTGC TGGAGCTGGA GACCAGGCTA
    AGGGAGGCAC ATAGAGATGA AATCCAGCGC AAATTCGATG CCCTTCGTAA CAGCTGTACT
    GTGATCACGG ACTTGGAGGA GCAGCTAAAC CAGCTGACGG AGGACAATGC CGAGCTCAAC
    AACCAAAACT TCTACTTGTC TAAACAACTC GATGAGGCCT CTGGTGCCAA CGACGAGATT
    GTGCAGCTGC GAAGTGAAGT GGACCATCTC CGCCGCGAGA TCACTGAGAG GGAGATGCAG
    CTCACTAGCC AGAAGCAAAC GATGGAGGCG CTGAAGACCA CGTGCACAAT GCTGGAGGAG
    CAGGTCATGG ACCTGGAGGC CCTGAACGAC GAGCTGCTGG AGAAGGAGCG GCAGTGGGAG
    GCCTGGAGGA GTGTCCTTGG TGACGAGAAG TCCCAGTTTG AGTGTCGGGT TCGAGAGTTG
    CAGAGGATGC TGGACACCGA AAAGCAGAGC AGGGCGAGGG CCGACCAGAG GATCACTGAG
    TCGCGCCAGG TGGTCGAGCT GGCAGTAAAG GAGCACAAGG CCGAGATCCT GGCCTTGCAG
    CAGGCTCTCA AGGAGCAGAA GCTGAAGGCC GAGAGCCTCT CCGACAAGCT CAATGATCTG
    GAGAAGAAGC ACGCCATGCT TGAAATGAAT GCCCGAAGTT TACAACAGAA GCTGGAGACA
    GAACGAGAGC TCAAACAAAG GCTTCTGGAA GAGCAAGCCA AGTTACAGCA GCAGATGGAC
    CTGCAGAAGA ATCATATTTT CCGTCTGACT CAAGGGCTGC AAGAAGCTCT GGATCGGGCT
    GATCTGCTGA AGACAGAAAG GAGTGACCTG GAATATCAGC TGGAAAACAT TCAGGTTCTC
    TATTCTCATG AGAAGGTGAA GATGGAAGGC ACTATTTCCC AGCAAACCAA ACTCATCGAT
    TTTCTGCAAG CCAAAATGGA CCAGCCTGCC AAAAAGAAAA AGGGTTTATT TAGTCGACGG
    AAAGAGGACC CTGCTTTGCC CACACAGGTT CCTCTGCAGT ACAATGAGCT GAAGGTGGCT
    CTGGAGAAGG AGAAAGCTCG CTGCGCGGAG CTCGAGGAAG CCCTGCAGAA GACCCGCATC
    GAGCTCCGCT CCGCCCGGGA GGAAGCTGCC CACCGGAAAG CTGCAGACCA CCCACACCCA
    TCTACGCCGG CTACCGCCAG GCAGCAGATC GCCATGTCCG CCATTGTGCG GTCACCCGAG
    CACCAGCCCA GCGCCCTGAG CCTGCTCGCC CCGCCGTCCA GCCGCAGGAA GGAGTCTTCC
    ACTCCAGAGG AATTCAGCCG GCGTCTTAAG GAGCGCATGC ACCACAACAT CCCTCACCGG
    TTCAACGTGG GACTGAACAT GCGAGCCACG AAGTGCGCCG TGTGTCTGGA TACCGTGCAC
    TTCGGGCGCC AGGCATCCAA GTGTCTCGAA TGTCAGGTCA TGTGCCACCC CAAGTGCTCC
    ACATGCTTGC CAGCCACCTG TGGCCTGCCA GCCGAATACG CCACACACTT CACCGAGGCC
    TTCTGCCGCG ACAAAATGAA CTCCCCGGGT CTGCAGTCCA AGGAGCCCAG CAGCAGCCTG
    CACCTGGAAG GGTGGATGAA GGTGCCCAGG AATAACAAGC GAGGACAGCA AGGCTGGGAC
    AGGAAGTACA TCGTTCTGGA GGGCTCCAAA GTCCTCATTT ATGACAACGA AGCCAGAGAA
    GCTGGACAGA GGCCGGTGGA AGAATTTGAG CTGTGCCTTC CCGACGGGGA CGTGTCTATT
    CATGGCGCCG TTGGTGCTTC TGAACTTGCA AACACAGCCA AAGCAGATGT CCCCTATGTC
    CTGAAGATGG AGTCTCACCC CCATACCACC TGCTGGCCTG GGAGGACCCT CTACTTGCTG
    GCTCCCAGCT TTCCCGACAA GCAGCGCTGG GTCACCGCCT TAGAGTCAGT TGTGGCAGGT
    GGGAGAGTGT CTAGGGAAAA GGCGGAAGCC GACGCTGGCC GTGACTGTAC TTCCTACGAG
    CCTCTGCCCG CGTGGGCTCA GAAATTGCTC GGAAACTCCC TGTTGAAACT GGAAGGTGAT
    GACCGTCTGG ACATGAACTG CACGCTGCCC TTCAGCGACC AGGTGGTGCT GGTGGGCACC
    GAGGAAGGGC TGTATGCGCT GAATGTCTTG AAGAACTCCC TCACCCACGT CCCAGGAATC
    GGAGCAGTCT TCCAGATTTA CATCATCAAG GACCTGGAGA AGCTCCTCAT GATCGCAGGA
    GAGGAGCGGG CCCTGTGTCT CGTGGATGTG AAGAAGGTGA AGCAGTCCCT CGCGCAGTCT
    CACCTCCCCG CCCAGCCAGA CATCTCGCCC AACATCTTTG AAGCGGTCAA GGGCTGCCAC
    TTGTTTGCTG CTGGCAAGAT TGAGAGCGGG CTCTGCATCT GTGCAGCCAT GCCCAGCAAA
    GTTGTCATTC TCCGCTACAA TGAAAACCTC AGCAAGTACT GCATTCGCAA AGAGATCGAG
    ACCTCGGAGC CCTGCAGCTG TATCCACTTC ACCAACTACA GTATCCTCAT CGGAACCAAT
    AAATTCTACG AAATCGACAT GAAGCAGTAC ACGCTAGAGG AATTCCTGGA TAAGAACGAC
    CATTCCTTGG CGCCTGCCGT GTTTGCCTCC TCTTCCAACA GCTTCCCCGT GTCCATCATG
    CAGGTGAACG GCGCGGGGCA GCGGGAGGAG TTCCTGCTCT GTTTCCACGA ATTCGGGGTG
    TTCGTGGATT CTTATGGAAG ACGCAGCCGC ACAGATGATC TCAAGTGGAG TCGCTTACCT
    CTGGCCTTTG CCTACAGAGA ACCCTATCTG TTTGTGACTC ACTTCAACTC ACTCGAAGTA
    ATTGAGATCC AGGCCCGCTC CTCTCTGGGG ACCCCCGCCC GAGCATATTT GGAAATCCCC
    AACCCACGCT ACCTGGGCCC TGCAATCTCC TCAGGAGCCA TCTACCTGGC GTCCTCATAT
    CAGGATAAGT TAAGGGTCAT CTGCTGCAAA GGAAACCTGG TGAAGGAGAC CGGCACCGAC
    CACCACCGTG GCCCATCCAC CTCCCGCAGC CCCAACAAGC GAGGCCCGCC GACGTACAAC
    GAGCACATCA CCAAGCGCGT GGCCTCAAGC CCGGCGCCTC CCGAAGGCCC CAGCCACCCT
    CGAGAGCCAA GCACACCCCA CCGCTACCGC GAGGGGCGGA CCGAGCTGCG CAGGGACAAG
    TCTCCGGGCC GCCCCCTGGA GCGCGAGAAG TCCCCCGGCC GGATGCTGAG CACGCGGAGG
    GAGCGCTCCC CCGGGCGGCT GTTTGAGGAC AGCAGCAGGG GCCGGCTGCC CGTGGGGGCC
    GTGAGGACCC CGCTGTCCCA GGTCAACAAG GTCTGGGACC AGTCTTCAGT ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.