sti cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	sti
Entrez Gene ID	39429
Full Name	sticky
Synonyms	CG10522,CIT-K,Citron K,DCK,Dmel\CG10522,STI,anon-WO0118547.388,cit,dck,l(3)7m-62,l(3)7m62,l(3)SG21,sti/Citron
General protein information	Preferred Names sticky Names CG10522 gene product from transcript CG10522-RA CG10522-PA CG10522-PB citron citron kinase drosophila citron kinase sti-PA sti-PB
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 69C4-69C4\|3-36.8 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_140327.2	NP_648584.1	sticky, isoform A
NM_001274839.1	NP_001261768.1	sticky, isoform B

Pathways from BioSystems

Homologies

Homo sapiens (human)	CIT	NP_001193928.1
Pan troglodytes (chimpanzee)	CIT	XP_003952361.1
Bos taurus (cattle)	CIT	XP_002694628.3
Rattus norvegicus (Norway rat)	Cit	NP_001025082.1
Drosophila melanogaster (fruit fly)	sti	NP_001261768.1
Canis lupus familiaris (dog)	CIT	XP_003433432.1
Mus musculus (house mouse)	Cit	NP_031734.3
Danio rerio (zebrafish)	LOC100329792	XP_005171785.1
	CIT	XP_001085560.1
Gallus gallus (chicken)	CIT	XP_004934462.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006932	XP_308829.4
Xenopus tropicalis (tropical clawed frog)	cit	NP_001120601.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

in this study we have provided evidence that ASPM controls spindle orientation by regulating the dynamics of astral MT and that CITK is a critical downstream partner of ASPM for this activity.
Title: ASPM and CITK regulate spindle orientation by affecting the dynamics of astral microtubules.

Data show that Sticky (Sti), tile ring component Citron kinase (CIT-K) interacts directly with two kinesins, Nebbish and Pavarotti and that in turn these kinesins interact with each other and with another central spindle protein, Fascetto.
Title: Citron kinase controls a molecular network required for midbody formation in cytokinesis.

The Sti/CIT-K maintains correct RhoA localization at the cleavage site, which is necessary for proper RhoA activity and contractile ring dynamics.
Title: Sticky/Citron kinase maintains proper RhoA localization at the cleavage site during cytokinesis.

sticky mutations can dominantly suppress a dFmr1 gain-of-function phenotype in the developing eye, while phenotypes produced by RNAi knock-down of sticky were enhanced by dFmr1 RNAi and a dFmr1 loss-of-function mutation
Title: Genetic and systems level analysis of Drosophila sticky/citron kinase and dFmr1 mutants reveals common regulation of genetic networks.

citron kinase has a critical role in the final steps of cytokinesis
Title: Drosophila citron kinase is required for the final steps of cytokinesis.

The following sti gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the sti cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa18365	NM_140327.2 Latest version!	Drosophila melanogaster sticky (sti), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OFa20842	NM_001274839.1 Latest version!	Drosophila melanogaster sticky (sti), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

sti ( NM_140327.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_648584.1 Drosophila melanogaster sticky (sti), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa18365
Clone ID Related Accession (Same CDS sequence)	NM_140327.2
Accession Version	NM_140327.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5565bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	sticky, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_140327.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCACCCA AGATGGAGCC GATTAGCGTG CGCACCGCGC GTCTGAACAA CCTGATTCTG 
GGCAAAGGAG CTGGCGTCTG TGCGAAGCCC GCTGGAAGCG CCTCCGGATC AGGTATTCCC 
GCCTCCACCA GGAGAAGCAT TGTACCCGTG AGCACCACTA GCGCCGCCGT GGCCGAGGCC 
ATCTGCCGCG AGGGACTCCT GGACGCCTTC TGTCTGCTGT ACAACGAGTG CGACAAGGAT 
ACGCTGAAGA AGCGCGATCG CAACATCGCC GAGTTTGTCA ACAAATTTCG TCCCATCATA 
GAGGAGACCC GCAAACTCAG GGTCAACGCA GATGACTTCC TCATCAAGAC GCTTATTGGC 
CAAGGATACT TTGGCAACGT TCACCTGGTG GTGGAGCGGC AAACCAACGA CATATACGCC 
ATGAAGAAGA TCAAAAAGTC GGTGGTGACG ACGTCACAAG TCAAGGAGGA GCGCGATATC 
ATGTCCATTC GCAACTCTGA ATGGCTAATC AATCTGCAGT ATGCCTTTCA GGATAACGAC 
AATCTGTACC TGGTCATGGA GTACATGCCC GGCGGAGACC TTCTCAGCTT GATGTCACGC 
CACGGCCCTT TCGATGAGGA CTTGGCTCGT TTCTATCTGG CCGAGCTCAC AGTGGCCCTG 
CACACATTGC ACGAGATGGG CTATGTGCAT CGGGATATAA AGCCTGAAAA TATACTGATT 
GATCGTTTTG GCCATATTAA ACTGGCTGAT TTCGGTAATG CCGCTGCCTT GGATCGCGAT 
GGCCATGTGC TGAGTTTGTC GCCGGTGGGA ACACCGGATT ACATAGCTCC AGAGCTGCTG 
CAAACCATAT CCACCTACAA GCTGTCCAAG TCGATGCACG ATGTCAGCTG CGATTATTGG 
TCCATGGGCA TTATAGGATA TGAGTTAATA TGCGAGACAA CACCTTTTCA CGAGGACAAT 
GTACACGAGA CATACTCCAA GATTCTCTCC CATTGCGAGG AGAGTCACCT TAAAGAGCTC 
ATCAGCTTTC CCGCCGACTT GAAGGTGTCT GTTAACTATA GAAATCTTAT TGAGTCACTG 
GTCACAAATC CTTCGAAGCG ATTGTCCTAT GAGCGCATTA AGAATCACCC CTTCTTTAGC 
GAAATTCCGT GGGGTTCCAT TCGCTCGCAG GTTCCACCGA TTATACCAAC TGTTAGGTCT 
GACGATGATA CCTCCAATTT CGAGGACGGA ATTCGTCATA AGACTCGCAG AGAACAGGGA 
GTTGCTAAAA AGTCCCTTAC GACTAACATG AAATCGAATG ATTTCAGTGG CAAGGATCTG 
CCATTCATTG GGTACAGTTT TGTGCACATG GAAAAGTCGG CAATTTCCGC TACCACCGAT 
GAAAAGCTTC AGGAGAAGCT AAAGGAACTG CTGCAGAAAC TGAAGACTAG AGAGAACGAG 
ATTTCCATGC TGAAACAAGA TCTTCTGCGG GCTCAGCAAT CGCTGAGGAA GACCGATAAC 
AAATCTCAGG TGGTAGCCGA TGCTAAAATG GAAATTAAGA AGCTGCAACA GATTATCAAG 
GAGAAAACAA TGGAGCTTAC CACTTGCAAG ACGCAAATTA AGACACTCCA AAGTTCGGCC 
AAAATAGACG AGGAAATGTG GTCCAAGAAG GAAGCCACTA TCACCGATCT CCTTCGCCTT 
AATCGACAAA AGTACGAAGA AGCCAAGATC GCTTCGGAGC AACGATATGA GAAACAATTG 
GCGGACAAAA AGCAAGAGCT GGCATCCACG CTGCAAAAGT TAGATGCCCG TGAGTTGGAG 
TTCAATGCCA AGTTCGAAGA GTGCAAGCAT TTGTCCATGA AGTTGCAAAA CTACAAGGAT 
ATGCTGCAGC AGATCAAGGA GCAGAATCTC AAGTCCGAGA CAAACCATGA GGAGCAGAGG 
CGCCAAATGG CCGAATTGTA TGAGCAAAAG CTAACAGATC TCCGGAAAAA GGTTCGCGAC 
TCGCAGGATA CAAACCGCCG CATGACAATG GAGATAAAGG AAATACGCAC CGAGCTGGAC 
GAGTCCATAT CCTCCAGCAA GTCCACCCAA GAAGCGAAGA ACGCTACCGA GCGGAATATC 
GAAGAAATTC TGCGGCGTCT GAACGAGGAG ATCGCCTCCA ATAATGAGCT TCATGCGGAA 
AAGGTCAAAC TGGAGACGAA GCTGCAGCTG AAGGAAAATG AAACTCAAGA AGTTAGAGCC 
GAATGTCATC GACTTGAGAG GGAATTGCAG CTTGCCGAAT GTCGCTGTCA GCTGGCTGAA 
TCCTCACTAG CCACCCAGGT TTCCCCCTAC GAAACTGCGC CTGGATCTTT GACCGAACTG 
AATGCCATTG AGGATCAACT TCGCGCCGAC TTGTTAGCCG CCAAAGAGAG TGAAAACCAT 
CAGAAGGGAC GCGCCGATCA ACTGCAGACG CTGGTTACCA AACTGGAGCA AATGCTGGAG 
CGCTTCAACG AGCAGAGTCT TTCACCCACG AAATCGCACT CATCGCGTAA GCAAGAAGGT 
GAAACCGTTG GTGATATGCT CGAGCGGCAA AACGAGAAGC TGGAGGATAA ATTGGCAGCA 
GTGCGAGAGC AGATGATTGT GGAACGTCAG GCAGCGCGTA CAGCGAATCT ATCGCTCTGG 
AAGGTGGAGA AACAGCTCGA GGAAGCTTTG TCCGAGAAGA AGTTACTGGC GCGTCGCATG 
GAACTCACAG AGGATCGGAT TAAAAAGGTG CAGAACGCAA GCGATGAAGC GCAGAGAATG 
CTCAAGACGT CACAGGAGGA AACTCGCCAG CGGGAGTCTC GCATCGAGGA GCTGAAGCAG 
GAACTGGCGG CCGCCAAAAG GGATGTCCTC AAGGAGCACC GCCAATGGGA AAAGGCCGAG 
CAGGAGCGCA TGAAGTGCAA GTCGGAGATA ATCGAGCACT TGGCCAATGT GCACAGATTA 
GAACAACAGG AGACGGAATT GCGCCAGAAA CTCAGGCAAA TCCAATCGCG ATTTGATGGT 
GTAACTTTGG AACAGAAGAA TACCATTCGA GAACTGCAGG AGGAGCGAGA AAAGAGCAGG 
AAAGCGAATG ACTCTTGTTT GGTCTTACAG AAGGAGCTGA AACAACTCAC CGATAACTTC 
CAGAGGTTGA AGTACGCTTG TAGCATTACC GACAGCCAGC TAACTGAGGT GGAGACCATG 
CTAAAATCGG AGCAGGAACG AAATAAATCC CAGAAATCTC AACTTGATAC CCTTCATGAG 
AAACTCAGAG AGCGCAACGA TCAATTAACT GATCTACGAA AGCAACTAAC TACGGTGGAA 
TCGGAAAAGC GTCTGGCGGA GCAGCGTGCT CAAGTGTTGG CATCGGAAAT CGATGAACTT 
CGTCTCAATC TCAAGGAGCA GCAGAAGAAG CTGGTGGCGC AGCAGGATCA GTTGGTGGAG 
CAGACTAACG CACTGTTTGC CACCCAAGAG AGAGCTGAAC TTTTGGATGG GCAAAATGCC 
AATTACGAAG CCCAAACTGC GGATTCGAAT CGCGAAATGG TTAGTTTGAA GGAGGAAAAT 
GCTAGAATTC TCAGCGAGCT GTTCCACAAG AAGGAGGAGG TTGGCAATCT TCAGGCGGAG 
ATAAGGGGCT TGGAGTCTGC GCAAGCCAAC TTGCACGCAG AGATCGATTC CCTTCAGGAC 
ACTTTGGCCG AGAAGGAGCA GTTCTACGTG CAGCGGGACA TTAAATCGAA TGCCACACTT 
GCGCAGCACA AAAAGCTGAT TGACTACCTT CAGCTCAAAG TTGAGGACCT ATCCGCCAAG 
AAGAAAAAGA CATTGGCGGA CAAACTTTTC GGTTCTAGCC ACACAAACAA GGAGAACGTG 
TCACCCAATG ACGTGGAATC TTCCATTCTG TATCGCGCTC TAAAGGAGGA ACTGAAGCGA 
GAACAAAAGA TGAACAGCTT GCTGAAGGAG CAGTTGGCTC AGTTGAATGG AACTGCAACC 
TTGCGCTCTC CACGCAAATC TGCAGCTGTT AATGGCGACT CCGATGCACC CAAACAGAGA 
CCCGTAAGCA TTGCTGCCTT GCCACGTTCT CCACAGAAGC AGCAGCAGCC CCTGAAGCGC 
ACCACCAGCC AGGTGGAGCT GAAGACGACG GCGGAGAAAC CGACTAAAGT AACCATTGAG 
AACCAGGCTC ATCACCGTTT CGAACTGGCC CTGCAGGAGT CCAAGGTGGA TGCCGTCAAC 
TGTGTAGTTT GCGAAAAGGC CGTCGTTGCC GGTTCGCCGT TCTGGAAATG CAAGGAGTGC 
AAAGATGTTA CGCATCGCAA GTGTCGCTCC AATGTTCAGA GCCATTGCGG ATCCACCAAG 
CCGACTGCAC CGTCAGCCGA TGATCTTAGC AGTATTCAAT CTGTCTCCAG CTTGACTTTG 
GATAGCGTAG ATGTTGCGGG TGGTACAACC AGCGGTGGCG AGTACATTGG ATCGCTGGTC 
TACAGTTCGG ATGGTGCAGA AGATCAGGCA CGGAAGGAGA TCGAAGTGAA CTGCGCCTTT 
GAAGTGGCCG AGCAGCAAAT ACTCCTATTG GGCTGTAACA CGGGACTGTA TGCGTATCAC 
TTGGACTCGC AGCGATTGGT GCACATTTCT GGTCTCGAAT CGGTGAGCTG CATGTCCATT 
TGCAAGCGCC TGGCTAAGGC TATAATGGTG GGCACAGTTG GCGAGAAGCT ATATCAATGT 
GACTACCGCC AGCTGGAGTC ACGCTGTCAG AGCTCCAGTT CGTGTCACAA GCCTGTGCTG 
GAAACGTCGG CCATTGAGCT GCCATTTGCC AATCGCACGC CATCGGAGAA ATGGAAACTA 
GTGCTGATCT CCGATGAGGC GGAGAACGCA TTGGATTCGG TGGCTATTGC GGCCACGTCC 
ACTAGGATTG TCATCTTGAA GTACGACCTT AAGCTGCACA TGTTTAAGCC AGTTCGAGCC 
TTGGACACGG CCACGCCGGT CACATCCATA TTCTTTACGC GTCACTCGGC CATTGTTAGC 
TCGGATAAGT TCTACGAGAT CGATTTGGAC AACTATGCGG CTGAGGAGTT TGTGGATCTG 
TCCGATAAGT CAATGGAGAG CACTGCCAAG TGCCAGCCCC TAACTGCGGT GCGCATCTCA 
CGACAAGAGT ATCTGCTCTG CTTTGCCGAA TATGGCGTCT TTGTGGACGA GTTTGGCTGC 
AGATCCAGGC CGTACGATTT GAACTGGGTC TATGCACCCA CTGGATTTGT TTACCGCGAT 
CCATTCCTAT TCATATCGCA CTATCAAAGC GTGCAGATAG TCCGTTTGCA TCGGTCGTTC 
AGCAAGGAAA TGGCCAGCGG GGATAACGCA TCGGAGAATT CGGAATCCCC GGAACTGCAG 
CGCGTCTACT TGCCCCACTA CATGTCCACG CTGCTGGCCA ACAGCGGAGA TGTCAATCTC 
TATGCCGTGG CCATCGATCA GAGGCCCTGC AATGGAACAC AGAAGATCTA CCATCTGGAC 
ACGATGCAGG CATTCAAGCA GAAGTTCAAT ATCTCCATGG AAACGCTGTC TTCAGTGGCC 
ACTTCTGTGA CTTTGGGCTC GACTGTCACC GGAAATAGCA TATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_648584.1
CDS	149..5713
Translation	MPPKMEPISVRTARLNNLILGKGAGVCAKPAGSASGSGIPASTRRSIVPVSTTSAAVAEAICREGLLDAFCLLYNECDKDTLKKRDRNIAEFVNKFRPIIEETRKLRVNADDFLIKTLIGQGYFGNVHLVVERQTNDIYAMKKIKKSVVTTSQVKEERDIMSIRNSEWLINLQYAFQDNDNLYLVMEYMPGGDLLSLMSRHGPFDEDLARFYLAELTVALHTLHEMGYVHRDIKPENILIDRFGHIKLADFGNAAALDRDGHVLSLSPVGTPDYIAPELLQTISTYKLSKSMHDVSCDYWSMGIIGYELICETTPFHEDNVHETYSKILSHCEESHLKELISFPADLKVSVNYRNLIESLVTNPSKRLSYERIKNHPFFSEIPWGSIRSQVPPIIPTVRSDDDTSNFEDGIRHKTRREQGVAKKSLTTNMKSNDFSGKDLPFIGYSFVHMEKSAISATTDEKLQEKLKELLQKLKTRENEISMLKQDLLRAQQSLRKTDNKSQVVADAKMEIKKLQQIIKEKTMELTTCKTQIKTLQSSAKIDEEMWSKKEATITDLLRLNRQKYEEAKIASEQRYEKQLADKKQELASTLQKLDARELEFNAKFEECKHLSMKLQNYKDMLQQIKEQNLKSETNHEEQRRQMAELYEQKLTDLRKKVRDSQDTNRRMTMEIKEIRTELDESISSSKSTQEAKNATERNIEEILRRLNEEIASNNELHAEKVKLETKLQLKENETQEVRAECHRLERELQLAECRCQLAESSLATQVSPYETAPGSLTELNAIEDQLRADLLAAKESENHQKGRADQLQTLVTKLEQMLERFNEQSLSPTKSHSSRKQEGETVGDMLERQNEKLEDKLAAVREQMIVERQAARTANLSLWKVEKQLEEALSEKKLLARRMELTEDRIKKVQNASDEAQRMLKTSQEETRQRESRIEELKQELAAAKRDVLKEHRQWEKAEQERMKCKSEIIEHLANVHRLEQQETELRQKLRQIQSRFDGVTLEQKNTIRELQEEREKSRKANDSCLVLQKELKQLTDNFQRLKYACSITDSQLTEVETMLKSEQERNKSQKSQLDTLHEKLRERNDQLTDLRKQLTTVESEKRLAEQRAQVLASEIDELRLNLKEQQKKLVAQQDQLVEQTNALFATQERAELLDGQNANYEAQTADSNREMVSLKEENARILSELFHKKEEVGNLQAEIRGLESAQANLHAEIDSLQDTLAEKEQFYVQRDIKSNATLAQHKKLIDYLQLKVEDLSAKKKKTLADKLFGSSHTNKENVSPNDVESSILYRALKEELKREQKMNSLLKEQLAQLNGTATLRSPRKSAAVNGDSDAPKQRPVSIAALPRSPQKQQQPLKRTTSQVELKTTAEKPTKVTIENQAHHRFELALQESKVDAVNCVVCEKAVVAGSPFWKCKECKDVTHRKCRSNVQSHCGSTKPTAPSADDLSSIQSVSSLTLDSVDVAGGTTSGGEYIGSLVYSSDGAEDQARKEIEVNCAFEVAEQQILLLGCNTGLYAYHLDSQRLVHISGLESVSCMSICKRLAKAIMVGTVGEKLYQCDYRQLESRCQSSSSCHKPVLETSAIELPFANRTPSEKWKLVLISDEAENALDSVAIAATSTRIVILKYDLKLHMFKPVRALDTATPVTSIFFTRHSAIVSSDKFYEIDLDNYAAEEFVDLSDKSMESTAKCQPLTAVRISRQEYLLCFAEYGVFVDEFGCRSRPYDLNWVYAPTGFVYRDPFLFISHYQSVQIVRLHRSFSKEMASGDNASENSESPELQRVYLPHYMSTLLANSGDVNLYAVAIDQRPCNGTQKIYHLDTMQAFKQKFNISMETLSSVATSVTLGSTVTGNSI

Target ORF information:

RefSeq Version	NM_140327.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster sticky (sti), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_140327.2

ORF Insert Sequence:

ATGCCACCCA AGATGGAGCC GATTAGCGTG CGCACCGCGC GTCTGAACAA CCTGATTCTG 
GGCAAAGGAG CTGGCGTCTG TGCGAAGCCC GCTGGAAGCG CCTCCGGATC AGGTATTCCC 
GCCTCCACCA GGAGAAGCAT TGTACCCGTG AGCACCACTA GCGCCGCCGT GGCCGAGGCC 
ATCTGCCGCG AGGGACTCCT GGACGCCTTC TGTCTGCTGT ACAACGAGTG CGACAAGGAT 
ACGCTGAAGA AGCGCGATCG CAACATCGCC GAGTTTGTCA ACAAATTTCG TCCCATCATA 
GAGGAGACCC GCAAACTCAG GGTCAACGCA GATGACTTCC TCATCAAGAC GCTTATTGGC 
CAAGGATACT TTGGCAACGT TCACCTGGTG GTGGAGCGGC AAACCAACGA CATATACGCC 
ATGAAGAAGA TCAAAAAGTC GGTGGTGACG ACGTCACAAG TCAAGGAGGA GCGCGATATC 
ATGTCCATTC GCAACTCTGA ATGGCTAATC AATCTGCAGT ATGCCTTTCA GGATAACGAC 
AATCTGTACC TGGTCATGGA GTACATGCCC GGCGGAGACC TTCTCAGCTT GATGTCACGC 
CACGGCCCTT TCGATGAGGA CTTGGCTCGT TTCTATCTGG CCGAGCTCAC AGTGGCCCTG 
CACACATTGC ACGAGATGGG CTATGTGCAT CGGGATATAA AGCCTGAAAA TATACTGATT 
GATCGTTTTG GCCATATTAA ACTGGCTGAT TTCGGTAATG CCGCTGCCTT GGATCGCGAT 
GGCCATGTGC TGAGTTTGTC GCCGGTGGGA ACACCGGATT ACATAGCTCC AGAGCTGCTG 
CAAACCATAT CCACCTACAA GCTGTCCAAG TCGATGCACG ATGTCAGCTG CGATTATTGG 
TCCATGGGCA TTATAGGATA TGAGTTAATA TGCGAGACAA CACCTTTTCA CGAGGACAAT 
GTACACGAGA CATACTCCAA GATTCTCTCC CATTGCGAGG AGAGTCACCT TAAAGAGCTC 
ATCAGCTTTC CCGCCGACTT GAAGGTGTCT GTTAACTATA GAAATCTTAT TGAGTCACTG 
GTCACAAATC CTTCGAAGCG ATTGTCCTAT GAGCGCATTA AGAATCACCC CTTCTTTAGC 
GAAATTCCGT GGGGTTCCAT TCGCTCGCAG GTTCCACCGA TTATACCAAC TGTTAGGTCT 
GACGATGATA CCTCCAATTT CGAGGACGGA ATTCGTCATA AGACTCGCAG AGAACAGGGA 
GTTGCTAAAA AGTCCCTTAC GACTAACATG AAATCGAATG ATTTCAGTGG CAAGGATCTG 
CCATTCATTG GGTACAGTTT TGTGCACATG GAAAAGTCGG CAATTTCCGC TACCACCGAT 
GAAAAGCTTC AGGAGAAGCT AAAGGAACTG CTGCAGAAAC TGAAGACTAG AGAGAACGAG 
ATTTCCATGC TGAAACAAGA TCTTCTGCGG GCTCAGCAAT CGCTGAGGAA GACCGATAAC 
AAATCTCAGG TGGTAGCCGA TGCTAAAATG GAAATTAAGA AGCTGCAACA GATTATCAAG 
GAGAAAACAA TGGAGCTTAC CACTTGCAAG ACGCAAATTA AGACACTCCA AAGTTCGGCC 
AAAATAGACG AGGAAATGTG GTCCAAGAAG GAAGCCACTA TCACCGATCT CCTTCGCCTT 
AATCGACAAA AGTACGAAGA AGCCAAGATC GCTTCGGAGC AACGATATGA GAAACAATTG 
GCGGACAAAA AGCAAGAGCT GGCATCCACG CTGCAAAAGT TAGATGCCCG TGAGTTGGAG 
TTCAATGCCA AGTTCGAAGA GTGCAAGCAT TTGTCCATGA AGTTGCAAAA CTACAAGGAT 
ATGCTGCAGC AGATCAAGGA GCAGAATCTC AAGTCCGAGA CAAACCATGA GGAGCAGAGG 
CGCCAAATGG CCGAATTGTA TGAGCAAAAG CTAACAGATC TCCGGAAAAA GGTTCGCGAC 
TCGCAGGATA CAAACCGCCG CATGACAATG GAGATAAAGG AAATACGCAC CGAGCTGGAC 
GAGTCCATAT CCTCCAGCAA GTCCACCCAA GAAGCGAAGA ACGCTACCGA GCGGAATATC 
GAAGAAATTC TGCGGCGTCT GAACGAGGAG ATCGCCTCCA ATAATGAGCT TCATGCGGAA 
AAGGTCAAAC TGGAGACGAA GCTGCAGCTG AAGGAAAATG AAACTCAAGA AGTTAGAGCC 
GAATGTCATC GACTTGAGAG GGAATTGCAG CTTGCCGAAT GTCGCTGTCA GCTGGCTGAA 
TCCTCACTAG CCACCCAGGT TTCCCCCTAC GAAACTGCGC CTGGATCTTT GACCGAACTG 
AATGCCATTG AGGATCAACT TCGCGCCGAC TTGTTAGCCG CCAAAGAGAG TGAAAACCAT 
CAGAAGGGAC GCGCCGATCA ACTGCAGACG CTGGTTACCA AACTGGAGCA AATGCTGGAG 
CGCTTCAACG AGCAGAGTCT TTCACCCACG AAATCGCACT CATCGCGTAA GCAAGAAGGT 
GAAACCGTTG GTGATATGCT CGAGCGGCAA AACGAGAAGC TGGAGGATAA ATTGGCAGCA 
GTGCGAGAGC AGATGATTGT GGAACGTCAG GCAGCGCGTA CAGCGAATCT ATCGCTCTGG 
AAGGTGGAGA AACAGCTCGA GGAAGCTTTG TCCGAGAAGA AGTTACTGGC GCGTCGCATG 
GAACTCACAG AGGATCGGAT TAAAAAGGTG CAGAACGCAA GCGATGAAGC GCAGAGAATG 
CTCAAGACGT CACAGGAGGA AACTCGCCAG CGGGAGTCTC GCATCGAGGA GCTGAAGCAG 
GAACTGGCGG CCGCCAAAAG GGATGTCCTC AAGGAGCACC GCCAATGGGA AAAGGCCGAG 
CAGGAGCGCA TGAAGTGCAA GTCGGAGATA ATCGAGCACT TGGCCAATGT GCACAGATTA 
GAACAACAGG AGACGGAATT GCGCCAGAAA CTCAGGCAAA TCCAATCGCG ATTTGATGGT 
GTAACTTTGG AACAGAAGAA TACCATTCGA GAACTGCAGG AGGAGCGAGA AAAGAGCAGG 
AAAGCGAATG ACTCTTGTTT GGTCTTACAG AAGGAGCTGA AACAACTCAC CGATAACTTC 
CAGAGGTTGA AGTACGCTTG TAGCATTACC GACAGCCAGC TAACTGAGGT GGAGACCATG 
CTAAAATCGG AGCAGGAACG AAATAAATCC CAGAAATCTC AACTTGATAC CCTTCATGAG 
AAACTCAGAG AGCGCAACGA TCAATTAACT GATCTACGAA AGCAACTAAC TACGGTGGAA 
TCGGAAAAGC GTCTGGCGGA GCAGCGTGCT CAAGTGTTGG CATCGGAAAT CGATGAACTT 
CGTCTCAATC TCAAGGAGCA GCAGAAGAAG CTGGTGGCGC AGCAGGATCA GTTGGTGGAG 
CAGACTAACG CACTGTTTGC CACCCAAGAG AGAGCTGAAC TTTTGGATGG GCAAAATGCC 
AATTACGAAG CCCAAACTGC GGATTCGAAT CGCGAAATGG TTAGTTTGAA GGAGGAAAAT 
GCTAGAATTC TCAGCGAGCT GTTCCACAAG AAGGAGGAGG TTGGCAATCT TCAGGCGGAG 
ATAAGGGGCT TGGAGTCTGC GCAAGCCAAC TTGCACGCAG AGATCGATTC CCTTCAGGAC 
ACTTTGGCCG AGAAGGAGCA GTTCTACGTG CAGCGGGACA TTAAATCGAA TGCCACACTT 
GCGCAGCACA AAAAGCTGAT TGACTACCTT CAGCTCAAAG TTGAGGACCT ATCCGCCAAG 
AAGAAAAAGA CATTGGCGGA CAAACTTTTC GGTTCTAGCC ACACAAACAA GGAGAACGTG 
TCACCCAATG ACGTGGAATC TTCCATTCTG TATCGCGCTC TAAAGGAGGA ACTGAAGCGA 
GAACAAAAGA TGAACAGCTT GCTGAAGGAG CAGTTGGCTC AGTTGAATGG AACTGCAACC 
TTGCGCTCTC CACGCAAATC TGCAGCTGTT AATGGCGACT CCGATGCACC CAAACAGAGA 
CCCGTAAGCA TTGCTGCCTT GCCACGTTCT CCACAGAAGC AGCAGCAGCC CCTGAAGCGC 
ACCACCAGCC AGGTGGAGCT GAAGACGACG GCGGAGAAAC CGACTAAAGT AACCATTGAG 
AACCAGGCTC ATCACCGTTT CGAACTGGCC CTGCAGGAGT CCAAGGTGGA TGCCGTCAAC 
TGTGTAGTTT GCGAAAAGGC CGTCGTTGCC GGTTCGCCGT TCTGGAAATG CAAGGAGTGC 
AAAGATGTTA CGCATCGCAA GTGTCGCTCC AATGTTCAGA GCCATTGCGG ATCCACCAAG 
CCGACTGCAC CGTCAGCCGA TGATCTTAGC AGTATTCAAT CTGTCTCCAG CTTGACTTTG 
GATAGCGTAG ATGTTGCGGG TGGTACAACC AGCGGTGGCG AGTACATTGG ATCGCTGGTC 
TACAGTTCGG ATGGTGCAGA AGATCAGGCA CGGAAGGAGA TCGAAGTGAA CTGCGCCTTT 
GAAGTGGCCG AGCAGCAAAT ACTCCTATTG GGCTGTAACA CGGGACTGTA TGCGTATCAC 
TTGGACTCGC AGCGATTGGT GCACATTTCT GGTCTCGAAT CGGTGAGCTG CATGTCCATT 
TGCAAGCGCC TGGCTAAGGC TATAATGGTG GGCACAGTTG GCGAGAAGCT ATATCAATGT 
GACTACCGCC AGCTGGAGTC ACGCTGTCAG AGCTCCAGTT CGTGTCACAA GCCTGTGCTG 
GAAACGTCGG CCATTGAGCT GCCATTTGCC AATCGCACGC CATCGGAGAA ATGGAAACTA 
GTGCTGATCT CCGATGAGGC GGAGAACGCA TTGGATTCGG TGGCTATTGC GGCCACGTCC 
ACTAGGATTG TCATCTTGAA GTACGACCTT AAGCTGCACA TGTTTAAGCC AGTTCGAGCC 
TTGGACACGG CCACGCCGGT CACATCCATA TTCTTTACGC GTCACTCGGC CATTGTTAGC 
TCGGATAAGT TCTACGAGAT CGATTTGGAC AACTATGCGG CTGAGGAGTT TGTGGATCTG 
TCCGATAAGT CAATGGAGAG CACTGCCAAG TGCCAGCCCC TAACTGCGGT GCGCATCTCA 
CGACAAGAGT ATCTGCTCTG CTTTGCCGAA TATGGCGTCT TTGTGGACGA GTTTGGCTGC 
AGATCCAGGC CGTACGATTT GAACTGGGTC TATGCACCCA CTGGATTTGT TTACCGCGAT 
CCATTCCTAT TCATATCGCA CTATCAAAGC GTGCAGATAG TCCGTTTGCA TCGGTCGTTC 
AGCAAGGAAA TGGCCAGCGG GGATAACGCA TCGGAGAATT CGGAATCCCC GGAACTGCAG 
CGCGTCTACT TGCCCCACTA CATGTCCACG CTGCTGGCCA ACAGCGGAGA TGTCAATCTC 
TATGCCGTGG CCATCGATCA GAGGCCCTGC AATGGAACAC AGAAGATCTA CCATCTGGAC 
ACGATGCAGG CATTCAAGCA GAAGTTCAAT ATCTCCATGG AAACGCTGTC TTCAGTGGCC 
ACTTCTGTGA CTTTGGGCTC GACTGTCACC GGAAATAGCA TATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sti ( NM_001274839.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261768.1 Drosophila melanogaster sticky (sti), transcript variant B, mRNA.

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CloneID	OFa20842
Clone ID Related Accession (Same CDS sequence)	NM_001274839.1
Accession Version	NM_001274839.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5577bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	sticky, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCACCCA AGATGGAGCC GATTAGCGTG CGCACCGCGC GTCTGAACAA CCTGATTCTG 
GGCAAAGGAG CTGGCGTCTG TGCGAAGCCC GCTGGAAGCG CCTCCGGATC AGGTATTCCC 
GCCTCCACCA GGAGAAGCAT TGTACCCGTG AGCACCACTA GCGCCGCCGT GGCCGAGGCC 
ATCTGCCGCG AGGGACTCCT GGACGCCTTC TGTCTGCTGT ACAACGAGTG CGACAAGGAT 
ACGCTGAAGA AGCGCGATCG CAACATCGCC GAGTTTGTCA ACAAATTTCG TCCCATCATA 
GAGGAGACCC GCAAACTCAG GGTCAACGCA GATGACTTCC TCATCAAGAC GCTTATTGGC 
CAAGGATACT TTGGCAACGT TCACCTGGTG GTGGAGCGGC AAACCAACGA CATATACGCC 
ATGAAGAAGA TCAAAAAGTC GGTGGTGACG ACGTCACAAG TCAAGGAGGA GCGCGATATC 
ATGTCCATTC GCAACTCTGA ATGGCTAATC AATCTGCAGT ATGCCTTTCA GGATAACGAC 
AATCTGTACC TGGTCATGGA GTACATGCCC GGCGGAGACC TTCTCAGCTT GATGTCACGC 
CACGGCCCTT TCGATGAGGA CTTGGCTCGT TTCTATCTGG CCGAGCTCAC AGTGGCCCTG 
CACACATTGC ACGAGATGGG CTATGTGCAT CGGGATATAA AGCCTGAAAA TATACTGATT 
GATCGTTTTG GCCATATTAA ACTGGCTGAT TTCGGTAATG CCGCTGCCTT GGATCGCGAT 
GGCCATGTGC TGAGTTTGTC GCCGGTGGGA ACACCGGATT ACATAGCTCC AGAGCTGCTG 
CAAACCATAT CCACCTACAA GCTGTCCAAG TCGATGCACG ATGTGAGTAG AATTGTCAGC 
TGCGATTATT GGTCCATGGG CATTATAGGA TATGAGTTAA TATGCGAGAC AACACCTTTT 
CACGAGGACA ATGTACACGA GACATACTCC AAGATTCTCT CCCATTGCGA GGAGAGTCAC 
CTTAAAGAGC TCATCAGCTT TCCCGCCGAC TTGAAGGTGT CTGTTAACTA TAGAAATCTT 
ATTGAGTCAC TGGTCACAAA TCCTTCGAAG CGATTGTCCT ATGAGCGCAT TAAGAATCAC 
CCCTTCTTTA GCGAAATTCC GTGGGGTTCC ATTCGCTCGC AGGTTCCACC GATTATACCA 
ACTGTTAGGT CTGACGATGA TACCTCCAAT TTCGAGGACG GAATTCGTCA TAAGACTCGC 
AGAGAACAGG GAGTTGCTAA AAAGTCCCTT ACGACTAACA TGAAATCGAA TGATTTCAGT 
GGCAAGGATC TGCCATTCAT TGGGTACAGT TTTGTGCACA TGGAAAAGTC GGCAATTTCC 
GCTACCACCG ATGAAAAGCT TCAGGAGAAG CTAAAGGAAC TGCTGCAGAA ACTGAAGACT 
AGAGAGAACG AGATTTCCAT GCTGAAACAA GATCTTCTGC GGGCTCAGCA ATCGCTGAGG 
AAGACCGATA ACAAATCTCA GGTGGTAGCC GATGCTAAAA TGGAAATTAA GAAGCTGCAA 
CAGATTATCA AGGAGAAAAC AATGGAGCTT ACCACTTGCA AGACGCAAAT TAAGACACTC 
CAAAGTTCGG CCAAAATAGA CGAGGAAATG TGGTCCAAGA AGGAAGCCAC TATCACCGAT 
CTCCTTCGCC TTAATCGACA AAAGTACGAA GAAGCCAAGA TCGCTTCGGA GCAACGATAT 
GAGAAACAAT TGGCGGACAA AAAGCAAGAG CTGGCATCCA CGCTGCAAAA GTTAGATGCC 
CGTGAGTTGG AGTTCAATGC CAAGTTCGAA GAGTGCAAGC ATTTGTCCAT GAAGTTGCAA 
AACTACAAGG ATATGCTGCA GCAGATCAAG GAGCAGAATC TCAAGTCCGA GACAAACCAT 
GAGGAGCAGA GGCGCCAAAT GGCCGAATTG TATGAGCAAA AGCTAACAGA TCTCCGGAAA 
AAGGTTCGCG ACTCGCAGGA TACAAACCGC CGCATGACAA TGGAGATAAA GGAAATACGC 
ACCGAGCTGG ACGAGTCCAT ATCCTCCAGC AAGTCCACCC AAGAAGCGAA GAACGCTACC 
GAGCGGAATA TCGAAGAAAT TCTGCGGCGT CTGAACGAGG AGATCGCCTC CAATAATGAG 
CTTCATGCGG AAAAGGTCAA ACTGGAGACG AAGCTGCAGC TGAAGGAAAA TGAAACTCAA 
GAAGTTAGAG CCGAATGTCA TCGACTTGAG AGGGAATTGC AGCTTGCCGA ATGTCGCTGT 
CAGCTGGCTG AATCCTCACT AGCCACCCAG GTTTCCCCCT ACGAAACTGC GCCTGGATCT 
TTGACCGAAC TGAATGCCAT TGAGGATCAA CTTCGCGCCG ACTTGTTAGC CGCCAAAGAG 
AGTGAAAACC ATCAGAAGGG ACGCGCCGAT CAACTGCAGA CGCTGGTTAC CAAACTGGAG 
CAAATGCTGG AGCGCTTCAA CGAGCAGAGT CTTTCACCCA CGAAATCGCA CTCATCGCGT 
AAGCAAGAAG GTGAAACCGT TGGTGATATG CTCGAGCGGC AAAACGAGAA GCTGGAGGAT 
AAATTGGCAG CAGTGCGAGA GCAGATGATT GTGGAACGTC AGGCAGCGCG TACAGCGAAT 
CTATCGCTCT GGAAGGTGGA GAAACAGCTC GAGGAAGCTT TGTCCGAGAA GAAGTTACTG 
GCGCGTCGCA TGGAACTCAC AGAGGATCGG ATTAAAAAGG TGCAGAACGC AAGCGATGAA 
GCGCAGAGAA TGCTCAAGAC GTCACAGGAG GAAACTCGCC AGCGGGAGTC TCGCATCGAG 
GAGCTGAAGC AGGAACTGGC GGCCGCCAAA AGGGATGTCC TCAAGGAGCA CCGCCAATGG 
GAAAAGGCCG AGCAGGAGCG CATGAAGTGC AAGTCGGAGA TAATCGAGCA CTTGGCCAAT 
GTGCACAGAT TAGAACAACA GGAGACGGAA TTGCGCCAGA AACTCAGGCA AATCCAATCG 
CGATTTGATG GTGTAACTTT GGAACAGAAG AATACCATTC GAGAACTGCA GGAGGAGCGA 
GAAAAGAGCA GGAAAGCGAA TGACTCTTGT TTGGTCTTAC AGAAGGAGCT GAAACAACTC 
ACCGATAACT TCCAGAGGTT GAAGTACGCT TGTAGCATTA CCGACAGCCA GCTAACTGAG 
GTGGAGACCA TGCTAAAATC GGAGCAGGAA CGAAATAAAT CCCAGAAATC TCAACTTGAT 
ACCCTTCATG AGAAACTCAG AGAGCGCAAC GATCAATTAA CTGATCTACG AAAGCAACTA 
ACTACGGTGG AATCGGAAAA GCGTCTGGCG GAGCAGCGTG CTCAAGTGTT GGCATCGGAA 
ATCGATGAAC TTCGTCTCAA TCTCAAGGAG CAGCAGAAGA AGCTGGTGGC GCAGCAGGAT 
CAGTTGGTGG AGCAGACTAA CGCACTGTTT GCCACCCAAG AGAGAGCTGA ACTTTTGGAT 
GGGCAAAATG CCAATTACGA AGCCCAAACT GCGGATTCGA ATCGCGAAAT GGTTAGTTTG 
AAGGAGGAAA ATGCTAGAAT TCTCAGCGAG CTGTTCCACA AGAAGGAGGA GGTTGGCAAT 
CTTCAGGCGG AGATAAGGGG CTTGGAGTCT GCGCAAGCCA ACTTGCACGC AGAGATCGAT 
TCCCTTCAGG ACACTTTGGC CGAGAAGGAG CAGTTCTACG TGCAGCGGGA CATTAAATCG 
AATGCCACAC TTGCGCAGCA CAAAAAGCTG ATTGACTACC TTCAGCTCAA AGTTGAGGAC 
CTATCCGCCA AGAAGAAAAA GACATTGGCG GACAAACTTT TCGGTTCTAG CCACACAAAC 
AAGGAGAACG TGTCACCCAA TGACGTGGAA TCTTCCATTC TGTATCGCGC TCTAAAGGAG 
GAACTGAAGC GAGAACAAAA GATGAACAGC TTGCTGAAGG AGCAGTTGGC TCAGTTGAAT 
GGAACTGCAA CCTTGCGCTC TCCACGCAAA TCTGCAGCTG TTAATGGCGA CTCCGATGCA 
CCCAAACAGA GACCCGTAAG CATTGCTGCC TTGCCACGTT CTCCACAGAA GCAGCAGCAG 
CCCCTGAAGC GCACCACCAG CCAGGTGGAG CTGAAGACGA CGGCGGAGAA ACCGACTAAA 
GTAACCATTG AGAACCAGGC TCATCACCGT TTCGAACTGG CCCTGCAGGA GTCCAAGGTG 
GATGCCGTCA ACTGTGTAGT TTGCGAAAAG GCCGTCGTTG CCGGTTCGCC GTTCTGGAAA 
TGCAAGGAGT GCAAAGATGT TACGCATCGC AAGTGTCGCT CCAATGTTCA GAGCCATTGC 
GGATCCACCA AGCCGACTGC ACCGTCAGCC GATGATCTTA GCAGTATTCA ATCTGTCTCC 
AGCTTGACTT TGGATAGCGT AGATGTTGCG GGTGGTACAA CCAGCGGTGG CGAGTACATT 
GGATCGCTGG TCTACAGTTC GGATGGTGCA GAAGATCAGG CACGGAAGGA GATCGAAGTG 
AACTGCGCCT TTGAAGTGGC CGAGCAGCAA ATACTCCTAT TGGGCTGTAA CACGGGACTG 
TATGCGTATC ACTTGGACTC GCAGCGATTG GTGCACATTT CTGGTCTCGA ATCGGTGAGC 
TGCATGTCCA TTTGCAAGCG CCTGGCTAAG GCTATAATGG TGGGCACAGT TGGCGAGAAG 
CTATATCAAT GTGACTACCG CCAGCTGGAG TCACGCTGTC AGAGCTCCAG TTCGTGTCAC 
AAGCCTGTGC TGGAAACGTC GGCCATTGAG CTGCCATTTG CCAATCGCAC GCCATCGGAG 
AAATGGAAAC TAGTGCTGAT CTCCGATGAG GCGGAGAACG CATTGGATTC GGTGGCTATT 
GCGGCCACGT CCACTAGGAT TGTCATCTTG AAGTACGACC TTAAGCTGCA CATGTTTAAG 
CCAGTTCGAG CCTTGGACAC GGCCACGCCG GTCACATCCA TATTCTTTAC GCGTCACTCG 
GCCATTGTTA GCTCGGATAA GTTCTACGAG ATCGATTTGG ACAACTATGC GGCTGAGGAG 
TTTGTGGATC TGTCCGATAA GTCAATGGAG AGCACTGCCA AGTGCCAGCC CCTAACTGCG 
GTGCGCATCT CACGACAAGA GTATCTGCTC TGCTTTGCCG AATATGGCGT CTTTGTGGAC 
GAGTTTGGCT GCAGATCCAG GCCGTACGAT TTGAACTGGG TCTATGCACC CACTGGATTT 
GTTTACCGCG ATCCATTCCT ATTCATATCG CACTATCAAA GCGTGCAGAT AGTCCGTTTG 
CATCGGTCGT TCAGCAAGGA AATGGCCAGC GGGGATAACG CATCGGAGAA TTCGGAATCC 
CCGGAACTGC AGCGCGTCTA CTTGCCCCAC TACATGTCCA CGCTGCTGGC CAACAGCGGA 
GATGTCAATC TCTATGCCGT GGCCATCGAT CAGAGGCCCT GCAATGGAAC ACAGAAGATC 
TACCATCTGG ACACGATGCA GGCATTCAAG CAGAAGTTCA ATATCTCCAT GGAAACGCTG 
TCTTCAGTGG CCACTTCTGT GACTTTGGGC TCGACTGTCA CCGGAAATAG CATATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261768.1
CDS	149..5725
Translation	MPPKMEPISVRTARLNNLILGKGAGVCAKPAGSASGSGIPASTRRSIVPVSTTSAAVAEAICREGLLDAFCLLYNECDKDTLKKRDRNIAEFVNKFRPIIEETRKLRVNADDFLIKTLIGQGYFGNVHLVVERQTNDIYAMKKIKKSVVTTSQVKEERDIMSIRNSEWLINLQYAFQDNDNLYLVMEYMPGGDLLSLMSRHGPFDEDLARFYLAELTVALHTLHEMGYVHRDIKPENILIDRFGHIKLADFGNAAALDRDGHVLSLSPVGTPDYIAPELLQTISTYKLSKSMHDVSRIVSCDYWSMGIIGYELICETTPFHEDNVHETYSKILSHCEESHLKELISFPADLKVSVNYRNLIESLVTNPSKRLSYERIKNHPFFSEIPWGSIRSQVPPIIPTVRSDDDTSNFEDGIRHKTRREQGVAKKSLTTNMKSNDFSGKDLPFIGYSFVHMEKSAISATTDEKLQEKLKELLQKLKTRENEISMLKQDLLRAQQSLRKTDNKSQVVADAKMEIKKLQQIIKEKTMELTTCKTQIKTLQSSAKIDEEMWSKKEATITDLLRLNRQKYEEAKIASEQRYEKQLADKKQELASTLQKLDARELEFNAKFEECKHLSMKLQNYKDMLQQIKEQNLKSETNHEEQRRQMAELYEQKLTDLRKKVRDSQDTNRRMTMEIKEIRTELDESISSSKSTQEAKNATERNIEEILRRLNEEIASNNELHAEKVKLETKLQLKENETQEVRAECHRLERELQLAECRCQLAESSLATQVSPYETAPGSLTELNAIEDQLRADLLAAKESENHQKGRADQLQTLVTKLEQMLERFNEQSLSPTKSHSSRKQEGETVGDMLERQNEKLEDKLAAVREQMIVERQAARTANLSLWKVEKQLEEALSEKKLLARRMELTEDRIKKVQNASDEAQRMLKTSQEETRQRESRIEELKQELAAAKRDVLKEHRQWEKAEQERMKCKSEIIEHLANVHRLEQQETELRQKLRQIQSRFDGVTLEQKNTIRELQEEREKSRKANDSCLVLQKELKQLTDNFQRLKYACSITDSQLTEVETMLKSEQERNKSQKSQLDTLHEKLRERNDQLTDLRKQLTTVESEKRLAEQRAQVLASEIDELRLNLKEQQKKLVAQQDQLVEQTNALFATQERAELLDGQNANYEAQTADSNREMVSLKEENARILSELFHKKEEVGNLQAEIRGLESAQANLHAEIDSLQDTLAEKEQFYVQRDIKSNATLAQHKKLIDYLQLKVEDLSAKKKKTLADKLFGSSHTNKENVSPNDVESSILYRALKEELKREQKMNSLLKEQLAQLNGTATLRSPRKSAAVNGDSDAPKQRPVSIAALPRSPQKQQQPLKRTTSQVELKTTAEKPTKVTIENQAHHRFELALQESKVDAVNCVVCEKAVVAGSPFWKCKECKDVTHRKCRSNVQSHCGSTKPTAPSADDLSSIQSVSSLTLDSVDVAGGTTSGGEYIGSLVYSSDGAEDQARKEIEVNCAFEVAEQQILLLGCNTGLYAYHLDSQRLVHISGLESVSCMSICKRLAKAIMVGTVGEKLYQCDYRQLESRCQSSSSCHKPVLETSAIELPFANRTPSEKWKLVLISDEAENALDSVAIAATSTRIVILKYDLKLHMFKPVRALDTATPVTSIFFTRHSAIVSSDKFYEIDLDNYAAEEFVDLSDKSMESTAKCQPLTAVRISRQEYLLCFAEYGVFVDEFGCRSRPYDLNWVYAPTGFVYRDPFLFISHYQSVQIVRLHRSFSKEMASGDNASENSESPELQRVYLPHYMSTLLANSGDVNLYAVAIDQRPCNGTQKIYHLDTMQAFKQKFNISMETLSSVATSVTLGSTVTGNSI

Target ORF information:

RefSeq Version	NM_001274839.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster sticky (sti), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274839.1

ORF Insert Sequence:

ATGCCACCCA AGATGGAGCC GATTAGCGTG CGCACCGCGC GTCTGAACAA CCTGATTCTG 
GGCAAAGGAG CTGGCGTCTG TGCGAAGCCC GCTGGAAGCG CCTCCGGATC AGGTATTCCC 
GCCTCCACCA GGAGAAGCAT TGTACCCGTG AGCACCACTA GCGCCGCCGT GGCCGAGGCC 
ATCTGCCGCG AGGGACTCCT GGACGCCTTC TGTCTGCTGT ACAACGAGTG CGACAAGGAT 
ACGCTGAAGA AGCGCGATCG CAACATCGCC GAGTTTGTCA ACAAATTTCG TCCCATCATA 
GAGGAGACCC GCAAACTCAG GGTCAACGCA GATGACTTCC TCATCAAGAC GCTTATTGGC 
CAAGGATACT TTGGCAACGT TCACCTGGTG GTGGAGCGGC AAACCAACGA CATATACGCC 
ATGAAGAAGA TCAAAAAGTC GGTGGTGACG ACGTCACAAG TCAAGGAGGA GCGCGATATC 
ATGTCCATTC GCAACTCTGA ATGGCTAATC AATCTGCAGT ATGCCTTTCA GGATAACGAC 
AATCTGTACC TGGTCATGGA GTACATGCCC GGCGGAGACC TTCTCAGCTT GATGTCACGC 
CACGGCCCTT TCGATGAGGA CTTGGCTCGT TTCTATCTGG CCGAGCTCAC AGTGGCCCTG 
CACACATTGC ACGAGATGGG CTATGTGCAT CGGGATATAA AGCCTGAAAA TATACTGATT 
GATCGTTTTG GCCATATTAA ACTGGCTGAT TTCGGTAATG CCGCTGCCTT GGATCGCGAT 
GGCCATGTGC TGAGTTTGTC GCCGGTGGGA ACACCGGATT ACATAGCTCC AGAGCTGCTG 
CAAACCATAT CCACCTACAA GCTGTCCAAG TCGATGCACG ATGTGAGTAG AATTGTCAGC 
TGCGATTATT GGTCCATGGG CATTATAGGA TATGAGTTAA TATGCGAGAC AACACCTTTT 
CACGAGGACA ATGTACACGA GACATACTCC AAGATTCTCT CCCATTGCGA GGAGAGTCAC 
CTTAAAGAGC TCATCAGCTT TCCCGCCGAC TTGAAGGTGT CTGTTAACTA TAGAAATCTT 
ATTGAGTCAC TGGTCACAAA TCCTTCGAAG CGATTGTCCT ATGAGCGCAT TAAGAATCAC 
CCCTTCTTTA GCGAAATTCC GTGGGGTTCC ATTCGCTCGC AGGTTCCACC GATTATACCA 
ACTGTTAGGT CTGACGATGA TACCTCCAAT TTCGAGGACG GAATTCGTCA TAAGACTCGC 
AGAGAACAGG GAGTTGCTAA AAAGTCCCTT ACGACTAACA TGAAATCGAA TGATTTCAGT 
GGCAAGGATC TGCCATTCAT TGGGTACAGT TTTGTGCACA TGGAAAAGTC GGCAATTTCC 
GCTACCACCG ATGAAAAGCT TCAGGAGAAG CTAAAGGAAC TGCTGCAGAA ACTGAAGACT 
AGAGAGAACG AGATTTCCAT GCTGAAACAA GATCTTCTGC GGGCTCAGCA ATCGCTGAGG 
AAGACCGATA ACAAATCTCA GGTGGTAGCC GATGCTAAAA TGGAAATTAA GAAGCTGCAA 
CAGATTATCA AGGAGAAAAC AATGGAGCTT ACCACTTGCA AGACGCAAAT TAAGACACTC 
CAAAGTTCGG CCAAAATAGA CGAGGAAATG TGGTCCAAGA AGGAAGCCAC TATCACCGAT 
CTCCTTCGCC TTAATCGACA AAAGTACGAA GAAGCCAAGA TCGCTTCGGA GCAACGATAT 
GAGAAACAAT TGGCGGACAA AAAGCAAGAG CTGGCATCCA CGCTGCAAAA GTTAGATGCC 
CGTGAGTTGG AGTTCAATGC CAAGTTCGAA GAGTGCAAGC ATTTGTCCAT GAAGTTGCAA 
AACTACAAGG ATATGCTGCA GCAGATCAAG GAGCAGAATC TCAAGTCCGA GACAAACCAT 
GAGGAGCAGA GGCGCCAAAT GGCCGAATTG TATGAGCAAA AGCTAACAGA TCTCCGGAAA 
AAGGTTCGCG ACTCGCAGGA TACAAACCGC CGCATGACAA TGGAGATAAA GGAAATACGC 
ACCGAGCTGG ACGAGTCCAT ATCCTCCAGC AAGTCCACCC AAGAAGCGAA GAACGCTACC 
GAGCGGAATA TCGAAGAAAT TCTGCGGCGT CTGAACGAGG AGATCGCCTC CAATAATGAG 
CTTCATGCGG AAAAGGTCAA ACTGGAGACG AAGCTGCAGC TGAAGGAAAA TGAAACTCAA 
GAAGTTAGAG CCGAATGTCA TCGACTTGAG AGGGAATTGC AGCTTGCCGA ATGTCGCTGT 
CAGCTGGCTG AATCCTCACT AGCCACCCAG GTTTCCCCCT ACGAAACTGC GCCTGGATCT 
TTGACCGAAC TGAATGCCAT TGAGGATCAA CTTCGCGCCG ACTTGTTAGC CGCCAAAGAG 
AGTGAAAACC ATCAGAAGGG ACGCGCCGAT CAACTGCAGA CGCTGGTTAC CAAACTGGAG 
CAAATGCTGG AGCGCTTCAA CGAGCAGAGT CTTTCACCCA CGAAATCGCA CTCATCGCGT 
AAGCAAGAAG GTGAAACCGT TGGTGATATG CTCGAGCGGC AAAACGAGAA GCTGGAGGAT 
AAATTGGCAG CAGTGCGAGA GCAGATGATT GTGGAACGTC AGGCAGCGCG TACAGCGAAT 
CTATCGCTCT GGAAGGTGGA GAAACAGCTC GAGGAAGCTT TGTCCGAGAA GAAGTTACTG 
GCGCGTCGCA TGGAACTCAC AGAGGATCGG ATTAAAAAGG TGCAGAACGC AAGCGATGAA 
GCGCAGAGAA TGCTCAAGAC GTCACAGGAG GAAACTCGCC AGCGGGAGTC TCGCATCGAG 
GAGCTGAAGC AGGAACTGGC GGCCGCCAAA AGGGATGTCC TCAAGGAGCA CCGCCAATGG 
GAAAAGGCCG AGCAGGAGCG CATGAAGTGC AAGTCGGAGA TAATCGAGCA CTTGGCCAAT 
GTGCACAGAT TAGAACAACA GGAGACGGAA TTGCGCCAGA AACTCAGGCA AATCCAATCG 
CGATTTGATG GTGTAACTTT GGAACAGAAG AATACCATTC GAGAACTGCA GGAGGAGCGA 
GAAAAGAGCA GGAAAGCGAA TGACTCTTGT TTGGTCTTAC AGAAGGAGCT GAAACAACTC 
ACCGATAACT TCCAGAGGTT GAAGTACGCT TGTAGCATTA CCGACAGCCA GCTAACTGAG 
GTGGAGACCA TGCTAAAATC GGAGCAGGAA CGAAATAAAT CCCAGAAATC TCAACTTGAT 
ACCCTTCATG AGAAACTCAG AGAGCGCAAC GATCAATTAA CTGATCTACG AAAGCAACTA 
ACTACGGTGG AATCGGAAAA GCGTCTGGCG GAGCAGCGTG CTCAAGTGTT GGCATCGGAA 
ATCGATGAAC TTCGTCTCAA TCTCAAGGAG CAGCAGAAGA AGCTGGTGGC GCAGCAGGAT 
CAGTTGGTGG AGCAGACTAA CGCACTGTTT GCCACCCAAG AGAGAGCTGA ACTTTTGGAT 
GGGCAAAATG CCAATTACGA AGCCCAAACT GCGGATTCGA ATCGCGAAAT GGTTAGTTTG 
AAGGAGGAAA ATGCTAGAAT TCTCAGCGAG CTGTTCCACA AGAAGGAGGA GGTTGGCAAT 
CTTCAGGCGG AGATAAGGGG CTTGGAGTCT GCGCAAGCCA ACTTGCACGC AGAGATCGAT 
TCCCTTCAGG ACACTTTGGC CGAGAAGGAG CAGTTCTACG TGCAGCGGGA CATTAAATCG 
AATGCCACAC TTGCGCAGCA CAAAAAGCTG ATTGACTACC TTCAGCTCAA AGTTGAGGAC 
CTATCCGCCA AGAAGAAAAA GACATTGGCG GACAAACTTT TCGGTTCTAG CCACACAAAC 
AAGGAGAACG TGTCACCCAA TGACGTGGAA TCTTCCATTC TGTATCGCGC TCTAAAGGAG 
GAACTGAAGC GAGAACAAAA GATGAACAGC TTGCTGAAGG AGCAGTTGGC TCAGTTGAAT 
GGAACTGCAA CCTTGCGCTC TCCACGCAAA TCTGCAGCTG TTAATGGCGA CTCCGATGCA 
CCCAAACAGA GACCCGTAAG CATTGCTGCC TTGCCACGTT CTCCACAGAA GCAGCAGCAG 
CCCCTGAAGC GCACCACCAG CCAGGTGGAG CTGAAGACGA CGGCGGAGAA ACCGACTAAA 
GTAACCATTG AGAACCAGGC TCATCACCGT TTCGAACTGG CCCTGCAGGA GTCCAAGGTG 
GATGCCGTCA ACTGTGTAGT TTGCGAAAAG GCCGTCGTTG CCGGTTCGCC GTTCTGGAAA 
TGCAAGGAGT GCAAAGATGT TACGCATCGC AAGTGTCGCT CCAATGTTCA GAGCCATTGC 
GGATCCACCA AGCCGACTGC ACCGTCAGCC GATGATCTTA GCAGTATTCA ATCTGTCTCC 
AGCTTGACTT TGGATAGCGT AGATGTTGCG GGTGGTACAA CCAGCGGTGG CGAGTACATT 
GGATCGCTGG TCTACAGTTC GGATGGTGCA GAAGATCAGG CACGGAAGGA GATCGAAGTG 
AACTGCGCCT TTGAAGTGGC CGAGCAGCAA ATACTCCTAT TGGGCTGTAA CACGGGACTG 
TATGCGTATC ACTTGGACTC GCAGCGATTG GTGCACATTT CTGGTCTCGA ATCGGTGAGC 
TGCATGTCCA TTTGCAAGCG CCTGGCTAAG GCTATAATGG TGGGCACAGT TGGCGAGAAG 
CTATATCAAT GTGACTACCG CCAGCTGGAG TCACGCTGTC AGAGCTCCAG TTCGTGTCAC 
AAGCCTGTGC TGGAAACGTC GGCCATTGAG CTGCCATTTG CCAATCGCAC GCCATCGGAG 
AAATGGAAAC TAGTGCTGAT CTCCGATGAG GCGGAGAACG CATTGGATTC GGTGGCTATT 
GCGGCCACGT CCACTAGGAT TGTCATCTTG AAGTACGACC TTAAGCTGCA CATGTTTAAG 
CCAGTTCGAG CCTTGGACAC GGCCACGCCG GTCACATCCA TATTCTTTAC GCGTCACTCG 
GCCATTGTTA GCTCGGATAA GTTCTACGAG ATCGATTTGG ACAACTATGC GGCTGAGGAG 
TTTGTGGATC TGTCCGATAA GTCAATGGAG AGCACTGCCA AGTGCCAGCC CCTAACTGCG 
GTGCGCATCT CACGACAAGA GTATCTGCTC TGCTTTGCCG AATATGGCGT CTTTGTGGAC 
GAGTTTGGCT GCAGATCCAG GCCGTACGAT TTGAACTGGG TCTATGCACC CACTGGATTT 
GTTTACCGCG ATCCATTCCT ATTCATATCG CACTATCAAA GCGTGCAGAT AGTCCGTTTG 
CATCGGTCGT TCAGCAAGGA AATGGCCAGC GGGGATAACG CATCGGAGAA TTCGGAATCC 
CCGGAACTGC AGCGCGTCTA CTTGCCCCAC TACATGTCCA CGCTGCTGGC CAACAGCGGA 
GATGTCAATC TCTATGCCGT GGCCATCGAT CAGAGGCCCT GCAATGGAAC ACAGAAGATC 
TACCATCTGG ACACGATGCA GGCATTCAAG CAGAAGTTCA ATATCTCCAT GGAAACGCTG 
TCTTCAGTGG CCACTTCTGT GACTTTGGGC TCGACTGTCA CCGGAAATAG CATATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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sti cDNA ORF clone, Drosophila melanogaster(fruit fly)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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