COL6A5 cDNA ORF clone, Bos taurus(cattle)

The following COL6A5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the COL6A5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa219332 XM_024997170.1
Latest version!
Bos taurus collagen type VI alpha 5 chain (COL6A5), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OBa219333 XM_024997173.1
Latest version!
Bos taurus collagen type VI alpha 5 chain (COL6A5), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OBa87867 XM_015474149.1
Latest version!
Bos taurus collagen type VI alpha 5 (COL6A5), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
OBa89499 XM_015462216.1
Latest version!
Bos taurus collagen type VI alpha 5 (COL6A5), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa219332
    Clone ID Related Accession (Same CDS sequence) XM_024997170.1
    Accession Version XM_024997170.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7821bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product collagen alpha-5(VI) chain isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    ATGAAGATCC TGCTAATTAT ATTTGTTCCT CTCTTCTGGA CTAAAACGCT GTCATATCAG 
    AGTCCAGGGC CAGGCCCTGA GTATGCTGAT GTGGTGTTTC TGATGGACAG CTCCGACCAC
    CTGGGAATTA AGTCCTTCCC GTTTGTGAAG ACCTTCATCA ACAAGATGAT CAGCAGCCTC
    CCCATAGAGG CTGACAAGTA CCACGTGGGC CTGGCCCAGT ACAGCGATGG GCTCCACAGA
    GAGTTCCTGC TCAGCGCCTT CAAGAGCAGG GGCCCCATGC TGAACCACCT CAAGAAGAAC
    TTCGGGTTCC TGGGGGGCTC CCTGCGGATT GGGAAGGCTC TCCAGGAGGT TCACAGGGCC
    TACTTCTCAA GCGGGAGGGA CAGGAAACAG TTTCCCCCGA TCCTGGTGGT CCTGGCTTCC
    GGAGAGTCTG AAGATGCTGT GGAAGAGGCC GCCGAAGCGC TGCGCAAAGA TGGGGTGAGG
    ATCGTCTCTG TGGGGATACA GGGGGCATCT GAGAAGACCC TGAAGGCCAT GGCCACGGGC
    CAGTTCCACT ACCGCCTCCG GACGGTCAGG GACCTCAGCG CATTCTCCCA AAACATGACG
    CAGATTCTCA AGGATGCAGC TCAGTACAAA GATGGAACAG CCTACTCTGA TATAGAAGTC
    CCCTTTCCCA GGGCCTGTAA GAAAGATTCA ATTGCCGACC TCATGTTTCT GGTGGATGAG
    GCCGTTGGGA CCACAGAGAA TCTAAGGCTC CTGCAGAACT TCCTGAAGAA CATCACCTCC
    TCCATGGATG TGCAAGACAG CTGCCTGCGG CTTGGACTTA TGAGCTACAG TGACAGAGTC
    GAGACGGTTT CTCCTCTGAG CTCGAGCACA ACCCAGTCTG AATTTCAGGA GCAAATCCAG
    AAGCTTTCTC TGCGGGCTGG GAAATCCAGT GCTGGAGCTG CCATTGATCA GCTGAGGAGG
    AAAGGCTTCT CAGCAGCACA TGGCAGCAGA CGGGCACAAG GAGTGCCCCA GATAGCAGTC
    CTGGTTACGG ACAGGCCGTC GGACGACAGC GTGCGTGAGG TGGCCCTCGA CCTCCGGCTG
    CAGGACGTAA CCGTGCTTGC CTTGGGCATT GAAGGGGCCA ACAAGACCCA GTTAGAAGAA
    ATAGTGTCTT ACCCTCCGGA GCAGACCATC TCCATGCTGC AGTCCTACGC AGACTTGGAT
    AATTATAGTA AGAAGTTCTT AAAAAAGCTC CAGAATGAAA TATGGTCCCA AGTCTCGGCT
    CGTGCTGAAC AAATGGAACT TGATAAAACT GGCTGTGTGG ACACAAAAGA GGCTGATCTC
    TATTTCCTCA TCGATGGCTC AAGCAGCATT CAGAAAAAAC ACTTTGAGCA AATCAGGGAA
    TTCATGTTGG CGGTGATAAG GATGTTTAGC ATTGGTCCAG ACAAAGTTCG AGTTGGAGCT
    GTGCAGTATT CCCATGAGAA AAAAGTGGAA TTTGATATCA GTGACTACCT TGATGATGTG
    AAATTAAGAA AGGCTGTTTT TAACATCAAG CAACTTGGGG GCGGAACTCG TACTGGGGCA
    GCCCTGGATT TCATGCTGCC ACTGATGAGG GAGGGAAGGA AGCAGAGGAT GAATGAGGTA
    CCCTGTCACC TCATTGTGCT GACCGATGGG ATATCTGGAG ATGCTGTCCT GGAACCTGCT
    GAGAGACTAA GGGCTGAAAA TATCACCATC CACGCGATTG GTATTGGGAA GGCTAACAGA
    ACACAACTGC AACAGATTGC GAGGGAAGAG GAAAGGGTTA ACTTTGGGCA GAACTTTGAT
    GCTTTAAAAA GCATCACGAA TGAAGTTGTT CGCAGTATCT GCACTGAGAA AGGATGTGAA
    AGCATGAAGG CTGACATCAT GTTTCTGGTG GACAGTTCTG GCAGTATAGG ACCTGAAAAT
    TTTGAGAAAA TGAAAACTTT CATGAAAAAC CTGTTAGATA AGATTCAGAT TGGTGAAGAC
    AAAAGCCGAA TTGGTGTAGT TCAATTCAGT CATTATAGTA AGGAAGAATT TCAGCTTGAC
    AAATACTTTA CACAAAAAGA AATTTCTGAT GCAATAGACA GCATGTCCCC CATCAGTGAC
    AATACTTTGA CTGGAAGTGC GATAACCTTT GTGGATCCAT ACTTCACCGA GTCCAAGGGG
    GGCCGCTCAA TGGTAAAAAA GTTTCTCATC CTTATCACTG ATGGAGAAGC CCAGGATGAT
    GTGAGAGACC CCGCAAAAGC TCTTCGGGAC AAAGGTGTGG TCATCTTCTC CGTTGGGGTA
    TACGGGGCCA ACAGGACTCA GCTGGAGGAG ATCAGCGGGG ATGGCAGCCT GATCTTCCAC
    GTTGAGAAAT TCGATGATCT AAAGGCAATA GAGAGTAAAC TCATCTTTCG CGTGTGTGCC
    CTTCATGATT GTAAACGGAT TCAGCAGTTA GATGTCGTGT TCGTGCTGGA CCACTCAGGC
    AGCATCAACC CCGAGGACCA AGAACACATG ATCAACCTCA CCATCCACCT GGTGAAGAAA
    TCAGACGTGG GCCTGGACCG CGTTCGGTTT GGAGCGCTCA GATACTCAGA TGACCCCGAG
    GTTCTTTTCT ACCTCAACAA GTACTCCAGT AAACCAGCCA TCATCGAGCA TCTGAGGAGG
    CGCAGGGATA CGGCGGGAAG AACCTACACC GCCAAGGCTC TGGAGCGCGC GAATGACATG
    TTCACAGAGG AACACGGCAG CCGTATCAAA CAAAATGTGA GGCAGATGCT GATCGTTATC
    ACAGATGGGG TATCTCACGA CAGAGATAAG CTCGGTGAGG CGGCCTCAAA ATTAAGAGCC
    AAAGGCGTCA TCATTTACGC AGTGGGTGTA GGTGCGGCTG ACCAAGCGGA ACTGGAGACC
    ATGGCGGGGG ATAAAAACTA CACTATCCAT GTCAGTGGTT TCGACAAACT GAAAGATATT
    TACCTGCCTG TGCAAGACAG TATGTGTAAC AACTCGCAAG AGGCCTGTGA CATTCCAGAA
    GCTGATGTGA TTTTCTTTTG TGATGGCTCT GACATGGTAT CTGATTCAGA CTTTGTTACC
    ATGACAACTT TCTTGTCAGA CTTAATTGAT AATTTTGACA TTCCATCTGA GAGGATGAAA
    ATTGGGATGG CCCAATTTGG GAGCCGCTAC CAAGAAATCA TCGAGTTACA GAACTCTCTG
    ACTAAAACCG AGTGGAAGTC TCGGATTCAA GCTATCACCA AGAGCAAGGG CCTTCCGCGA
    ATAGACTTGG CCCTGAGAAA AGTGGGCATT ATGTTTGAGC CATCCGCTGG CGGCAGAAGG
    AGCGCTGGTG TCCCTCAGAC TTTGGTGGTC ATCACATCTG GGGGTCCCCG CTATGATGTG
    TCAAAGGCAG TGGAGGCCCT GAGAGAAGCT GGCATTTGTA TTCTGGCTCT GGGCATAGGA
    GATGTTTACA AGGAACAGCT CCTGCCGATA ACGGGCAGTT CTGAAAAAAT CATCACTTTT
    CAAGACTTTG ATAAATTAAA GAATGTGGAT GTGAAAAAAA GAATGATCCG TGAAATCTGC
    AAGAGTTGCG GGAAAACCAA CTGCTTTATG GAGATTGTGG TTGGCTTCGA CATTTCCACT
    CACCTGCCGG GTCAGCCATT GTTCCATGGC CACCCCCGGC TGCAGTCATA CCTCCCAAGC
    ATCTTAGAGG ACATCACCTC CATCAGGGGT GTCAGCTGCG GGGCAGGTGC AGAGGTGCAG
    GTGAGCGTGG CCTTTAAGGT GAACAGTGGC CAGAACGTCC CTGCCGAGTT CCAAATCTAC
    CAGCAAACAA TATTTGACAG CCTCCTGCAA GTCACCGTCA ACGGACCGAC TCACCTGGAT
    GCGCAGTTCC TGCAGTCCAT GTGGGACACC TTTGAGAATG TGTCTACATC CCAAGGACAG
    GTGTTGCTCA TCTTTTCAGA TGGTCTTGGG GGTGAAAGCA AAATGATGCT TGAAGATCAA
    TCGGACAGGC TCAGGGAAGC AGGACTTGAT GCTTTGCTGG TAGTGTCCCT AAATGAAACC
    GCTCACGATG AGTTTTCCAG CTTCGAATTT GGAAAAGGGT TTGACTACAG GACTCACCTG
    ACCATTGGAA TGAAGGAACT GGGCAGTATG CTATCACAGT ATCTGGGGAA CATTGCAGAG
    AGGACGTGTT GCTGTCAGTT TTGCAAATGC CCAGGGATTC CAGGACCTCA GGGGAGCCAA
    GGGCCACAAG CCTCCCAGGG CTCTCCAGGT GTGAAAGGCA GCAGAGGACA CAGGGGAGAG
    GAGGGAGAGC CTGGAGCTCG AGGAGAAATT GGACCCCAAG GAAACAGAGG GAATGCAGGA
    TGTCCAGGGC AGCCAGGTCA AAAGGGAGCC AGAGGATTTT CTGGATATAA GGGAGAACAC
    GGAGATGATG GGATTGATGG ACTGGATGGG GAAGAGGGCT TTCATGGACT TCCTGGGAAA
    AAGGGAGAAA AAGGTGATCC AGGTTCCCAG GGCAGCCCAG GTTCCAGAGG CCTCCCTGGG
    GGATATGGGG ACAAGGGTTT CCCAGGGGAT CCTGGTAATC CAGGACAAAG CAGTACCATC
    AAAGGACAAA AGGGCTCCAA AGGAGAACAA GGAAGACAAG GTAGAACCGG GCAGAAAGGG
    ACGCAAGGCA GTCCTAGCTC CAGAGGGAGC AGGGGAAGAG AAGGCCAGAG GGGAGTCCAG
    GGTGCCTTGG GGGAACCGGG CACACCTGGA CCTCAAGGTG AATCAGGAGC TGAAGGATTG
    CAAGGATCAC AGGGAGCCAG TGGACCTCCG GGCGGGAAAG GAGAGAAGGG AAGCGAGGGA
    CGCAAAGGAC CTCAGGGTGC TCCTGGACCA GTGGGAGAGA AAGGGAGTGT TGGAAGGCCT
    GGTCCTTTGG GGAAAAAGGG AGAACCTGGG ATTCCTGGAG ATCCAGGGCC AGCGGGGCAA
    GTGGGACAGC GAGGAAAGCA GGGTGACTAT GGCATCCCAG GCTATGGTCC TATGGGAGGA
    AAAGGAGTCA AGGGTCCACG AGGATTCCCT GGAGATATGG GCCAAAAGGG TGATACTGGT
    GATCCTGGAA TTCCTGGGGG GCCTGGACCC AAAGGATTTA GGGGATTCAC ACTCACGGTA
    GGCCTGAAAG GTGAAGCGGG CTCTCCTGGA AACCAAGGCC CCCCTGGACG GAGAGGCCCT
    AAAGGAATGG CAGGGAAGCC CATATATTCT CAATGTGATC TAATCCAGTT CACGCGGGAC
    CATAGTCCTT GTTGGAAAGA AAAGTGTCCT GTGTATCCAA CAGAGCTGGT ATTTGCCCTG
    GACCAGTCCA ACGATATCAC AGAGCAGAGA TTTAATGAAA TGAGGGACAT CATCACTTCT
    ATTGTCAATG ACCTTCACAT CAGGGAAAGT AATTGCCCCG AGGGAGCCAG AGTTGTTGTG
    GTTTCCTACA ACTCGGGCAC CGGCTACCTC ATCCATGGGT CTGACTACCG TAGCAAGAAG
    CAGCTTCTTC AGCTTCTCTC CCAAATAAAA TATCAAAGTT CTGGGGAAGT CCGAGACATT
    GGGAACGCGA TGAGGTTTGT GGCTCGGAAC ATCTTTAAGC GGACGCCTGC AGGAGCCAAT
    GTGCAGAGAG TTGCTGTGTT TTTTAGCAAC GGACAAGCTG CGAGCAGGTC ATCTGTCATC
    ACAGCCACCA TGGAGTTCAG TGCCTTGGAT ATCAGTCCTG CAGTCTTCGC TTTTAATGAA
    AAGGTTTACC TCGATGAGGC TTTTGGGTTT GACAACACAG GAACATTTCA GGTGCTTTCT
    GTTCCTCCAA ATGGGGAATA TGATCCATTA GAAAGGCTTC GGCGCTGTAC ACTTTGCTAT
    GATAAATGTT TTCCAAATAC TTGCAAGAAA GAAATTGTCA TACCTGAAAA TTCATACATG
    GATGTCGCCT TCCTCTTAGA CAATTCTAGA AATGTAGCAA GTGATGAGTT TAAGGATATG
    AAAGCCTTGG TGAGCTCAAT GCTTGACAAC TTTGAAATTG CCTCAGACCC TATAGTCTCA
    GACTCTGGTG ATAGGATTGC CTTGTTGAGC TATTCTCCTT GGGATAGGAG AAAGAAGAAT
    GTGGTAAAAA CAGAGTTTGA GTTTACAACT TATAACAGTC AAGCTCTGAT GAAAAGGTAC
    ATTGAGACTA ACCTCCGACA GCTAAATGGG GAAGCCACCA TTGGCCATGC CCTACTGTGG
    GTCGTGGAGA ATCTCTTCCC AGCAACGCCG AAGCTAAGAA AATACAGGGT CATCTTTGTG
    GTCTCCGCTG GAGAGAATCG TGAGAGAAAG GAATTCTTGA AGAAGATGGC TCTGAGGGCC
    AAGTGTCAAG GCTATGTCAT ATTTGTGATT TCCCTGGGTT TGACATCCGA GGAGGACATG
    GAGGAGCTGG CCAGCCACCC GCTTGATCAC CATCTGATAC AGCTGGGGAG AATTCATAAA
    CCAAATCTGG ATTATGTTGT GAAGTTTTTA AAGCCGTTTG TGTACTCAGT GAGACGAGGA
    TTCAATCAGT ACCCACCACC AGTGCTTGAG AACATCTGCC AACTCATTGA TTCAGAAGAC
    GAGGAAAATC AAGGCATCGG TCTCCCATTT ACTCCTGAAC CTTATGAGAT TTCTTCAGGA
    GAGAACATCA TTGGCCAGGA ATTGAGTGCT CTGAGAGATT CATCTTTTGT ATTGGAGGAC
    AATGGAAGCG ACCATTTGGT TTATGTTCCA AGCCAGATGC TTATGCCACA GAAATTAATG
    ACTAAGTATG AAAAAGATTG GAATTCTGAA GCAACTGCAA GTCTCACATC TGGACATGAA
    AACCTTGGCA GAAAAGAAGA ACCAGGTCTT ACTTATGAAA GTAGAGATGC CTCTCTTCAA
    GAATATTACA TGGATGTGAC TTTTCTCATA GATGCTTCCA AAAGAATAGG AAGTGATGAG
    TTTAAGGAAG TGAAAGCTTT TGTAACCTCA GTGCTTGATT ATTTTCATCT GGCCCCAGAT
    CCACTGACCT CCACCTTAGG AGACAGAATA GCAGTCCTGA CCTACTCTCC TCCAGGCTAT
    ATGCCCAACA CAGAAAAATG CCCCGTCTAC CTAGAATTTG ATTTGGTCAC TTATAACAAT
    ATACACCAAA TGAAACATCA TCTCCAAGAC TCCCTTCAGC AGCTCAATGG AGATGTTTTT
    ATAGGCCACG CCCTTCAGTG GACACTTGAC AATGTCTTTG TAGGAACTCC CAACCTGAGG
    AGAAACAAAG TTATCTTTGT TATATCCGCT GGTGAAACCA ACCCTTTAGA CAGGGAAGTC
    TTAAGAAATG TATCTCTGAG AGCCAAGTGT CAGGGCTACT CCATATTTGT GTTTTCTTTT
    GGTCCTCTAC ACAATGACAA GGAATTAGAA GAATTAGCCA GCCACCCACT GGATCATCAC
    TTAGTCCAGC TTGGCCGAAC CCACAAGCCA GATTTGAACT ATATCATCAA ATTTATCAAG
    CCATTTGTTC ATTCAATCAG ACGTGGCATC AACAAATACC CCCCTGCAGA CATGAAACCC
    AAATGTGTTA ACATCACCTC TCCCACTCCA GAGAATAATG GCATAGGAAA CACTGTATTC
    CTTCTTCCTG AGGTATATGA AATTGAGATG GAGAACAGTG AGCTTTCGGG TGACCCCGAT
    TCCCAGCAGC AGCATTTCTT TGAAATAGGG AACAGTCATA GTAACAGTTC TGGTATCAAC
    ACTAATTTGA TCCAGAGGTT ATACATGCTC TTTTCATCTG GGGAACTGAT GATGCAAGAC
    AAGAAAGAGG CACATTCAGA AGAAATTACA TCTCCAGCAA ATGACAGACA AGATGAAAAA
    GATGGTGAAG ACACAAGATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024852938.1
    CDS193..8013
    Translation

    Target ORF information:

    RefSeq Version XM_024997170.1
    Organism Bos taurus(cattle)
    Definition Bos taurus collagen type VI alpha 5 chain (COL6A5), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024997170.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    ATGAAGATCC TGCTAATTAT ATTTGTTCCT CTCTTCTGGA CTAAAACGCT GTCATATCAG 
    AGTCCAGGGC CAGGCCCTGA GTATGCTGAT GTGGTGTTTC TGATGGACAG CTCCGACCAC
    CTGGGAATTA AGTCCTTCCC GTTTGTGAAG ACCTTCATCA ACAAGATGAT CAGCAGCCTC
    CCCATAGAGG CTGACAAGTA CCACGTGGGC CTGGCCCAGT ACAGCGATGG GCTCCACAGA
    GAGTTCCTGC TCAGCGCCTT CAAGAGCAGG GGCCCCATGC TGAACCACCT CAAGAAGAAC
    TTCGGGTTCC TGGGGGGCTC CCTGCGGATT GGGAAGGCTC TCCAGGAGGT TCACAGGGCC
    TACTTCTCAA GCGGGAGGGA CAGGAAACAG TTTCCCCCGA TCCTGGTGGT CCTGGCTTCC
    GGAGAGTCTG AAGATGCTGT GGAAGAGGCC GCCGAAGCGC TGCGCAAAGA TGGGGTGAGG
    ATCGTCTCTG TGGGGATACA GGGGGCATCT GAGAAGACCC TGAAGGCCAT GGCCACGGGC
    CAGTTCCACT ACCGCCTCCG GACGGTCAGG GACCTCAGCG CATTCTCCCA AAACATGACG
    CAGATTCTCA AGGATGCAGC TCAGTACAAA GATGGAACAG CCTACTCTGA TATAGAAGTC
    CCCTTTCCCA GGGCCTGTAA GAAAGATTCA ATTGCCGACC TCATGTTTCT GGTGGATGAG
    GCCGTTGGGA CCACAGAGAA TCTAAGGCTC CTGCAGAACT TCCTGAAGAA CATCACCTCC
    TCCATGGATG TGCAAGACAG CTGCCTGCGG CTTGGACTTA TGAGCTACAG TGACAGAGTC
    GAGACGGTTT CTCCTCTGAG CTCGAGCACA ACCCAGTCTG AATTTCAGGA GCAAATCCAG
    AAGCTTTCTC TGCGGGCTGG GAAATCCAGT GCTGGAGCTG CCATTGATCA GCTGAGGAGG
    AAAGGCTTCT CAGCAGCACA TGGCAGCAGA CGGGCACAAG GAGTGCCCCA GATAGCAGTC
    CTGGTTACGG ACAGGCCGTC GGACGACAGC GTGCGTGAGG TGGCCCTCGA CCTCCGGCTG
    CAGGACGTAA CCGTGCTTGC CTTGGGCATT GAAGGGGCCA ACAAGACCCA GTTAGAAGAA
    ATAGTGTCTT ACCCTCCGGA GCAGACCATC TCCATGCTGC AGTCCTACGC AGACTTGGAT
    AATTATAGTA AGAAGTTCTT AAAAAAGCTC CAGAATGAAA TATGGTCCCA AGTCTCGGCT
    CGTGCTGAAC AAATGGAACT TGATAAAACT GGCTGTGTGG ACACAAAAGA GGCTGATCTC
    TATTTCCTCA TCGATGGCTC AAGCAGCATT CAGAAAAAAC ACTTTGAGCA AATCAGGGAA
    TTCATGTTGG CGGTGATAAG GATGTTTAGC ATTGGTCCAG ACAAAGTTCG AGTTGGAGCT
    GTGCAGTATT CCCATGAGAA AAAAGTGGAA TTTGATATCA GTGACTACCT TGATGATGTG
    AAATTAAGAA AGGCTGTTTT TAACATCAAG CAACTTGGGG GCGGAACTCG TACTGGGGCA
    GCCCTGGATT TCATGCTGCC ACTGATGAGG GAGGGAAGGA AGCAGAGGAT GAATGAGGTA
    CCCTGTCACC TCATTGTGCT GACCGATGGG ATATCTGGAG ATGCTGTCCT GGAACCTGCT
    GAGAGACTAA GGGCTGAAAA TATCACCATC CACGCGATTG GTATTGGGAA GGCTAACAGA
    ACACAACTGC AACAGATTGC GAGGGAAGAG GAAAGGGTTA ACTTTGGGCA GAACTTTGAT
    GCTTTAAAAA GCATCACGAA TGAAGTTGTT CGCAGTATCT GCACTGAGAA AGGATGTGAA
    AGCATGAAGG CTGACATCAT GTTTCTGGTG GACAGTTCTG GCAGTATAGG ACCTGAAAAT
    TTTGAGAAAA TGAAAACTTT CATGAAAAAC CTGTTAGATA AGATTCAGAT TGGTGAAGAC
    AAAAGCCGAA TTGGTGTAGT TCAATTCAGT CATTATAGTA AGGAAGAATT TCAGCTTGAC
    AAATACTTTA CACAAAAAGA AATTTCTGAT GCAATAGACA GCATGTCCCC CATCAGTGAC
    AATACTTTGA CTGGAAGTGC GATAACCTTT GTGGATCCAT ACTTCACCGA GTCCAAGGGG
    GGCCGCTCAA TGGTAAAAAA GTTTCTCATC CTTATCACTG ATGGAGAAGC CCAGGATGAT
    GTGAGAGACC CCGCAAAAGC TCTTCGGGAC AAAGGTGTGG TCATCTTCTC CGTTGGGGTA
    TACGGGGCCA ACAGGACTCA GCTGGAGGAG ATCAGCGGGG ATGGCAGCCT GATCTTCCAC
    GTTGAGAAAT TCGATGATCT AAAGGCAATA GAGAGTAAAC TCATCTTTCG CGTGTGTGCC
    CTTCATGATT GTAAACGGAT TCAGCAGTTA GATGTCGTGT TCGTGCTGGA CCACTCAGGC
    AGCATCAACC CCGAGGACCA AGAACACATG ATCAACCTCA CCATCCACCT GGTGAAGAAA
    TCAGACGTGG GCCTGGACCG CGTTCGGTTT GGAGCGCTCA GATACTCAGA TGACCCCGAG
    GTTCTTTTCT ACCTCAACAA GTACTCCAGT AAACCAGCCA TCATCGAGCA TCTGAGGAGG
    CGCAGGGATA CGGCGGGAAG AACCTACACC GCCAAGGCTC TGGAGCGCGC GAATGACATG
    TTCACAGAGG AACACGGCAG CCGTATCAAA CAAAATGTGA GGCAGATGCT GATCGTTATC
    ACAGATGGGG TATCTCACGA CAGAGATAAG CTCGGTGAGG CGGCCTCAAA ATTAAGAGCC
    AAAGGCGTCA TCATTTACGC AGTGGGTGTA GGTGCGGCTG ACCAAGCGGA ACTGGAGACC
    ATGGCGGGGG ATAAAAACTA CACTATCCAT GTCAGTGGTT TCGACAAACT GAAAGATATT
    TACCTGCCTG TGCAAGACAG TATGTGTAAC AACTCGCAAG AGGCCTGTGA CATTCCAGAA
    GCTGATGTGA TTTTCTTTTG TGATGGCTCT GACATGGTAT CTGATTCAGA CTTTGTTACC
    ATGACAACTT TCTTGTCAGA CTTAATTGAT AATTTTGACA TTCCATCTGA GAGGATGAAA
    ATTGGGATGG CCCAATTTGG GAGCCGCTAC CAAGAAATCA TCGAGTTACA GAACTCTCTG
    ACTAAAACCG AGTGGAAGTC TCGGATTCAA GCTATCACCA AGAGCAAGGG CCTTCCGCGA
    ATAGACTTGG CCCTGAGAAA AGTGGGCATT ATGTTTGAGC CATCCGCTGG CGGCAGAAGG
    AGCGCTGGTG TCCCTCAGAC TTTGGTGGTC ATCACATCTG GGGGTCCCCG CTATGATGTG
    TCAAAGGCAG TGGAGGCCCT GAGAGAAGCT GGCATTTGTA TTCTGGCTCT GGGCATAGGA
    GATGTTTACA AGGAACAGCT CCTGCCGATA ACGGGCAGTT CTGAAAAAAT CATCACTTTT
    CAAGACTTTG ATAAATTAAA GAATGTGGAT GTGAAAAAAA GAATGATCCG TGAAATCTGC
    AAGAGTTGCG GGAAAACCAA CTGCTTTATG GAGATTGTGG TTGGCTTCGA CATTTCCACT
    CACCTGCCGG GTCAGCCATT GTTCCATGGC CACCCCCGGC TGCAGTCATA CCTCCCAAGC
    ATCTTAGAGG ACATCACCTC CATCAGGGGT GTCAGCTGCG GGGCAGGTGC AGAGGTGCAG
    GTGAGCGTGG CCTTTAAGGT GAACAGTGGC CAGAACGTCC CTGCCGAGTT CCAAATCTAC
    CAGCAAACAA TATTTGACAG CCTCCTGCAA GTCACCGTCA ACGGACCGAC TCACCTGGAT
    GCGCAGTTCC TGCAGTCCAT GTGGGACACC TTTGAGAATG TGTCTACATC CCAAGGACAG
    GTGTTGCTCA TCTTTTCAGA TGGTCTTGGG GGTGAAAGCA AAATGATGCT TGAAGATCAA
    TCGGACAGGC TCAGGGAAGC AGGACTTGAT GCTTTGCTGG TAGTGTCCCT AAATGAAACC
    GCTCACGATG AGTTTTCCAG CTTCGAATTT GGAAAAGGGT TTGACTACAG GACTCACCTG
    ACCATTGGAA TGAAGGAACT GGGCAGTATG CTATCACAGT ATCTGGGGAA CATTGCAGAG
    AGGACGTGTT GCTGTCAGTT TTGCAAATGC CCAGGGATTC CAGGACCTCA GGGGAGCCAA
    GGGCCACAAG CCTCCCAGGG CTCTCCAGGT GTGAAAGGCA GCAGAGGACA CAGGGGAGAG
    GAGGGAGAGC CTGGAGCTCG AGGAGAAATT GGACCCCAAG GAAACAGAGG GAATGCAGGA
    TGTCCAGGGC AGCCAGGTCA AAAGGGAGCC AGAGGATTTT CTGGATATAA GGGAGAACAC
    GGAGATGATG GGATTGATGG ACTGGATGGG GAAGAGGGCT TTCATGGACT TCCTGGGAAA
    AAGGGAGAAA AAGGTGATCC AGGTTCCCAG GGCAGCCCAG GTTCCAGAGG CCTCCCTGGG
    GGATATGGGG ACAAGGGTTT CCCAGGGGAT CCTGGTAATC CAGGACAAAG CAGTACCATC
    AAAGGACAAA AGGGCTCCAA AGGAGAACAA GGAAGACAAG GTAGAACCGG GCAGAAAGGG
    ACGCAAGGCA GTCCTAGCTC CAGAGGGAGC AGGGGAAGAG AAGGCCAGAG GGGAGTCCAG
    GGTGCCTTGG GGGAACCGGG CACACCTGGA CCTCAAGGTG AATCAGGAGC TGAAGGATTG
    CAAGGATCAC AGGGAGCCAG TGGACCTCCG GGCGGGAAAG GAGAGAAGGG AAGCGAGGGA
    CGCAAAGGAC CTCAGGGTGC TCCTGGACCA GTGGGAGAGA AAGGGAGTGT TGGAAGGCCT
    GGTCCTTTGG GGAAAAAGGG AGAACCTGGG ATTCCTGGAG ATCCAGGGCC AGCGGGGCAA
    GTGGGACAGC GAGGAAAGCA GGGTGACTAT GGCATCCCAG GCTATGGTCC TATGGGAGGA
    AAAGGAGTCA AGGGTCCACG AGGATTCCCT GGAGATATGG GCCAAAAGGG TGATACTGGT
    GATCCTGGAA TTCCTGGGGG GCCTGGACCC AAAGGATTTA GGGGATTCAC ACTCACGGTA
    GGCCTGAAAG GTGAAGCGGG CTCTCCTGGA AACCAAGGCC CCCCTGGACG GAGAGGCCCT
    AAAGGAATGG CAGGGAAGCC CATATATTCT CAATGTGATC TAATCCAGTT CACGCGGGAC
    CATAGTCCTT GTTGGAAAGA AAAGTGTCCT GTGTATCCAA CAGAGCTGGT ATTTGCCCTG
    GACCAGTCCA ACGATATCAC AGAGCAGAGA TTTAATGAAA TGAGGGACAT CATCACTTCT
    ATTGTCAATG ACCTTCACAT CAGGGAAAGT AATTGCCCCG AGGGAGCCAG AGTTGTTGTG
    GTTTCCTACA ACTCGGGCAC CGGCTACCTC ATCCATGGGT CTGACTACCG TAGCAAGAAG
    CAGCTTCTTC AGCTTCTCTC CCAAATAAAA TATCAAAGTT CTGGGGAAGT CCGAGACATT
    GGGAACGCGA TGAGGTTTGT GGCTCGGAAC ATCTTTAAGC GGACGCCTGC AGGAGCCAAT
    GTGCAGAGAG TTGCTGTGTT TTTTAGCAAC GGACAAGCTG CGAGCAGGTC ATCTGTCATC
    ACAGCCACCA TGGAGTTCAG TGCCTTGGAT ATCAGTCCTG CAGTCTTCGC TTTTAATGAA
    AAGGTTTACC TCGATGAGGC TTTTGGGTTT GACAACACAG GAACATTTCA GGTGCTTTCT
    GTTCCTCCAA ATGGGGAATA TGATCCATTA GAAAGGCTTC GGCGCTGTAC ACTTTGCTAT
    GATAAATGTT TTCCAAATAC TTGCAAGAAA GAAATTGTCA TACCTGAAAA TTCATACATG
    GATGTCGCCT TCCTCTTAGA CAATTCTAGA AATGTAGCAA GTGATGAGTT TAAGGATATG
    AAAGCCTTGG TGAGCTCAAT GCTTGACAAC TTTGAAATTG CCTCAGACCC TATAGTCTCA
    GACTCTGGTG ATAGGATTGC CTTGTTGAGC TATTCTCCTT GGGATAGGAG AAAGAAGAAT
    GTGGTAAAAA CAGAGTTTGA GTTTACAACT TATAACAGTC AAGCTCTGAT GAAAAGGTAC
    ATTGAGACTA ACCTCCGACA GCTAAATGGG GAAGCCACCA TTGGCCATGC CCTACTGTGG
    GTCGTGGAGA ATCTCTTCCC AGCAACGCCG AAGCTAAGAA AATACAGGGT CATCTTTGTG
    GTCTCCGCTG GAGAGAATCG TGAGAGAAAG GAATTCTTGA AGAAGATGGC TCTGAGGGCC
    AAGTGTCAAG GCTATGTCAT ATTTGTGATT TCCCTGGGTT TGACATCCGA GGAGGACATG
    GAGGAGCTGG CCAGCCACCC GCTTGATCAC CATCTGATAC AGCTGGGGAG AATTCATAAA
    CCAAATCTGG ATTATGTTGT GAAGTTTTTA AAGCCGTTTG TGTACTCAGT GAGACGAGGA
    TTCAATCAGT ACCCACCACC AGTGCTTGAG AACATCTGCC AACTCATTGA TTCAGAAGAC
    GAGGAAAATC AAGGCATCGG TCTCCCATTT ACTCCTGAAC CTTATGAGAT TTCTTCAGGA
    GAGAACATCA TTGGCCAGGA ATTGAGTGCT CTGAGAGATT CATCTTTTGT ATTGGAGGAC
    AATGGAAGCG ACCATTTGGT TTATGTTCCA AGCCAGATGC TTATGCCACA GAAATTAATG
    ACTAAGTATG AAAAAGATTG GAATTCTGAA GCAACTGCAA GTCTCACATC TGGACATGAA
    AACCTTGGCA GAAAAGAAGA ACCAGGTCTT ACTTATGAAA GTAGAGATGC CTCTCTTCAA
    GAATATTACA TGGATGTGAC TTTTCTCATA GATGCTTCCA AAAGAATAGG AAGTGATGAG
    TTTAAGGAAG TGAAAGCTTT TGTAACCTCA GTGCTTGATT ATTTTCATCT GGCCCCAGAT
    CCACTGACCT CCACCTTAGG AGACAGAATA GCAGTCCTGA CCTACTCTCC TCCAGGCTAT
    ATGCCCAACA CAGAAAAATG CCCCGTCTAC CTAGAATTTG ATTTGGTCAC TTATAACAAT
    ATACACCAAA TGAAACATCA TCTCCAAGAC TCCCTTCAGC AGCTCAATGG AGATGTTTTT
    ATAGGCCACG CCCTTCAGTG GACACTTGAC AATGTCTTTG TAGGAACTCC CAACCTGAGG
    AGAAACAAAG TTATCTTTGT TATATCCGCT GGTGAAACCA ACCCTTTAGA CAGGGAAGTC
    TTAAGAAATG TATCTCTGAG AGCCAAGTGT CAGGGCTACT CCATATTTGT GTTTTCTTTT
    GGTCCTCTAC ACAATGACAA GGAATTAGAA GAATTAGCCA GCCACCCACT GGATCATCAC
    TTAGTCCAGC TTGGCCGAAC CCACAAGCCA GATTTGAACT ATATCATCAA ATTTATCAAG
    CCATTTGTTC ATTCAATCAG ACGTGGCATC AACAAATACC CCCCTGCAGA CATGAAACCC
    AAATGTGTTA ACATCACCTC TCCCACTCCA GAGAATAATG GCATAGGAAA CACTGTATTC
    CTTCTTCCTG AGGTATATGA AATTGAGATG GAGAACAGTG AGCTTTCGGG TGACCCCGAT
    TCCCAGCAGC AGCATTTCTT TGAAATAGGG AACAGTCATA GTAACAGTTC TGGTATCAAC
    ACTAATTTGA TCCAGAGGTT ATACATGCTC TTTTCATCTG GGGAACTGAT GATGCAAGAC
    AAGAAAGAGG CACATTCAGA AGAAATTACA TCTCCAGCAA ATGACAGACA AGATGAAAAA
    GATGGTGAAG ACACAAGATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa219333
    Clone ID Related Accession (Same CDS sequence) XM_024997173.1
    Accession Version XM_024997173.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6792bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product collagen alpha-5(VI) chain isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    ATGAAGATCC TGCTAATTAT ATTTGTTCCT CTCTTCTGGA CTAAAACGCT GTCATATCAG 
    AGTCCAGGGC CAGGCCCTGA GTATGCTGAT GTGGTGTTTC TGATGGACAG CTCCGACCAC
    CTGGGAATTA AGTCCTTCCC GTTTGTGAAG ACCTTCATCA ACAAGATGAT CAGCAGCCTC
    CCCATAGAGG CTGACAAGTA CCACGTGGGC CTGGCCCAGT ACAGCGATGG GCTCCACAGA
    GAGTTCCTGC TCAGCGCCTT CAAGAGCAGG GGCCCCATGC TGAACCACCT CAAGAAGAAC
    TTCGGGTTCC TGGGGGGCTC CCTGCGGATT GGGAAGGCTC TCCAGGAGGT TCACAGGGCC
    TACTTCTCAA GCGGGAGGGA CAGGAAACAG TTTCCCCCGA TCCTGGTGGT CCTGGCTTCC
    GGAGAGTCTG AAGATGCTGT GGAAGAGGCC GCCGAAGCGC TGCGCAAAGA TGGGGTGAGG
    ATCGTCTCTG TGGGGATACA GGGGGCATCT GAGAAGACCC TGAAGGCCAT GGCCACGGGC
    CAGTTCCACT ACCGCCTCCG GACGGTCAGG GACCTCAGCG CATTCTCCCA AAACATGACG
    CAGATTCTCA AGGATGCAGC TCAGTACAAA GATGGAACAG CCTACTCTGA TATAGAAGTC
    CCCTTTCCCA GGGCCTGTAA GAAAGATTCA ATTGCCGACC TCATGTTTCT GGTGGATGAG
    GCCGTTGGGA CCACAGAGAA TCTAAGGCTC CTGCAGAACT TCCTGAAGAA CATCACCTCC
    TCCATGGATG TGCAAGACAG CTGCCTGCGG CTTGGACTTA TGAGCTACAG TGACAGAGTC
    GAGACGGTTT CTCCTCTGAG CTCGAGCACA ACCCAGTCTG AATTTCAGGA GCAAATCCAG
    AAGCTTTCTC TGCGGGCTGG GAAATCCAGT GCTGGAGCTG CCATTGATCA GCTGAGGAGG
    AAAGGCTTCT CAGCAGCACA TGGCAGCAGA CGGGCACAAG GAGTGCCCCA GATAGCAGTC
    CTGGTTACGG ACAGGCCGTC GGACGACAGC GTGCGTGAGG TGGCCCTCGA CCTCCGGCTG
    CAGGACGTAA CCGTGCTTGC CTTGGGCATT GAAGGGGCCA ACAAGACCCA GTTAGAAGAA
    ATAGTGTCTT ACCCTCCGGA GCAGACCATC TCCATGCTGC AGTCCTACGC AGACTTGGAT
    AATTATAGTA AGAAGTTCTT AAAAAAGCTC CAGAATGAAA TATGGTCCCA AGTCTCGGCT
    CGTGCTGAAC AAATGGAACT TGATAAAACT GGCTGTGTGG ACACAAAAGA GGCTGATCTC
    TATTTCCTCA TCGATGGCTC AAGCAGCATT CAGAAAAAAC ACTTTGAGCA AATCAGGGAA
    TTCATGTTGG CGGTGATAAG GATGTTTAGC ATTGGTCCAG ACAAAGTTCG AGTTGGAGCT
    GTGCAGTATT CCCATGAGAA AAAAGTGGAA TTTGATATCA GTGACTACCT TGATGATGTG
    AAATTAAGAA AGGCTGTTTT TAACATCAAG CAACTTGGGG GCGGAACTCG TACTGGGGCA
    GCCCTGGATT TCATGCTGCC ACTGATGAGG GAGGGAAGGA AGCAGAGGAT GAATGAGGTA
    CCCTGTCACC TCATTGTGCT GACCGATGGG ATATCTGGAG ATGCTGTCCT GGAACCTGCT
    GAGAGACTAA GGGCTGAAAA TATCACCATC CACGCGATTG GTATTGGGAA GGCTAACAGA
    ACACAACTGC AACAGATTGC GAGGGAAGAG GAAAGGGTTA ACTTTGGGCA GAACTTTGAT
    GCTTTAAAAA GCATCACGAA TGAAGTTGTT CGCAGTATCT GCACTGAGAA AGGATGTGAA
    AGCATGAAGG CTGACATCAT GTTTCTGGTG GACAGTTCTG GCAGTATAGG ACCTGAAAAT
    TTTGAGAAAA TGAAAACTTT CATGAAAAAC CTGTTAGATA AGATTCAGAT TGGTGAAGAC
    AAAAGCCGAA TTGGTGTAGT TCAATTCAGT CATTATAGTA AGGAAGAATT TCAGCTTGAC
    AAATACTTTA CACAAAAAGA AATTTCTGAT GCAATAGACA GCATGTCCCC CATCAGTGAC
    AATACTTTGA CTGGAAGTGC GATAACCTTT GTGGATCCAT ACTTCACCGA GTCCAAGGGG
    GGCCGCTCAA TGGTAAAAAA GTTTCTCATC CTTATCACTG ATGGAGAAGC CCAGGATGAT
    GTGAGAGACC CCGCAAAAGC TCTTCGGGAC AAAGGTGTGG TCATCTTCTC CGTTGGGGTA
    TACGGGGCCA ACAGGACTCA GCTGGAGGAG ATCAGCGGGG ATGGCAGCCT GATCTTCCAC
    GTTGAGAAAT TCGATGATCT AAAGGCAATA GAGAGTAAAC TCATCTTTCG CGTGTGTGCC
    CTTCATGATT GTAAACGGAT TCAGCAGTTA GATGTCGTGT TCGTGCTGGA CCACTCAGGC
    AGCATCAACC CCGAGGACCA AGAACACATG ATCAACCTCA CCATCCACCT GGTGAAGAAA
    TCAGACGTGG GCCTGGACCG CGTTCGGTTT GGAGCGCTCA GATACTCAGA TGACCCCGAG
    GTTCTTTTCT ACCTCAACAA GTACTCCAGT AAACCAGCCA TCATCGAGCA TCTGAGGAGG
    CGCAGGGATA CGGCGGGAAG AACCTACACC GCCAAGGCTC TGGAGCGCGC GAATGACATG
    TTCACAGAGG AACACGGCAG CCGTATCAAA CAAAATGTGA GGCAGATGCT GATCGTTATC
    ACAGATGGGG TATCTCACGA CAGAGATAAG CTCGGTGAGG CGGCCTCAAA ATTAAGAGCC
    AAAGGCGTCA TCATTTACGC AGTGGGTGTA GGTGCGGCTG ACCAAGCGGA ACTGGAGACC
    ATGGCGGGGG ATAAAAACTA CACTATCCAT GTCAGTGGTT TCGACAAACT GAAAGATATT
    TACCTGCCTG TGCAAGACAG TATGTGTAAC AACTCGCAAG AGGCCTGTGA CATTCCAGAA
    GCTGATGTGA TTTTCTTTTG TGATGGCTCT GACATGGTAT CTGATTCAGA CTTTGTTACC
    ATGACAACTT TCTTGTCAGA CTTAATTGAT AATTTTGACA TTCCATCTGA GAGGATGAAA
    ATTGGGATGG CCCAATTTGG GAGCCGCTAC CAAGAAATCA TCGAGTTACA GAACTCTCTG
    ACTAAAACCG AGTGGAAGTC TCGGATTCAA GCTATCACCA AGAGCAAGGG CCTTCCGCGA
    ATAGACTTGG CCCTGAGAAA AGTGGGCATT ATGTTTGAGC CATCCGCTGG CGGCAGAAGG
    AGCGCTGGTG TCCCTCAGAC TTTGGTGGTC ATCACATCTG GGGGTCCCCG CTATGATGTG
    TCAAAGGCAG TGGAGGCCCT GAGAGAAGCT GGCATTTGTA TTCTGGCTCT GGGCATAGGA
    GATGTTTACA AGGAACAGCT CCTGCCGATA ACGGGCAGTT CTGAAAAAAT CATCACTTTT
    CAAGACTTTG ATAAATTAAA GAATGTGGAT GTGAAAAAAA GAATGATCCG TGAAATCTGC
    AAGAGTTGCG GGAAAACCAA CTGCTTTATG GAGATTGTGG TTGGCTTCGA CATTTCCACT
    CACCTGCCGG GTCAGCCATT GTTCCATGGC CACCCCCGGC TGCAGTCATA CCTCCCAAGC
    ATCTTAGAGG ACATCACCTC CATCAGGGGT GTCAGCTGCG GGGCAGGTGC AGAGGTGCAG
    GTGAGCGTGG CCTTTAAGGT GAACAGTGGC CAGAACGTCC CTGCCGAGTT CCAAATCTAC
    CAGCAAACAA TATTTGACAG CCTCCTGCAA GTCACCGTCA ACGGACCGAC TCACCTGGAT
    GCGCAGTTCC TGCAGTCCAT GTGGGACACC TTTGAGAATG TGTCTACATC CCAAGGACAG
    GTGTTGCTCA TCTTTTCAGA TGGTCTTGGG GGTGAAAGCA AAATGATGCT TGAAGATCAA
    TCGGACAGGC TCAGGGAAGC AGGACTTGAT GCTTTGCTGG TAGTGTCCCT AAATGAAACC
    GCTCACGATG AGTTTTCCAG CTTCGAATTT GGAAAAGGGT TTGACTACAG GACTCACCTG
    ACCATTGGAA TGAAGGAACT GGGCAGTATG CTATCACAGT ATCTGGGGAA CATTGCAGAG
    AGGACGTGTT GCTGTCAGTT TTGCAAATGC CCAGGGATTC CAGGACCTCA GGGGAGCCAA
    GGGCCACAAG CCTCCCAGGG CTCTCCAGGT GTGAAAGGCA GCAGAGGACA CAGGGGAGAG
    GAGGGAGAGC CTGGAGCTCG AGGAGAAATT GGACCCCAAG GAAACAGAGG GAATGCAGGA
    TGTCCAGGGC AGCCAGGTCA AAAGGGAGCC AGAGGATTTT CTGGATATAA GGGAGAACAC
    GGAGATGATG GGATTGATGG ACTGGATGGG GAAGAGGGCT TTCATGGACT TCCTGGGAAA
    AAGGGAGAAA AAGGTGATCC AGGTTCCCAG GGCAGCCCAG GTTCCAGAGG CCTCCCTGGG
    GGATATGGGG ACAAGGGTTT CCCAGGGGAT CCTGGTAATC CAGGACAAAG CAGTACCATC
    AAAGGACAAA AGGGCTCCAA AGGAGAACAA GGAAGACAAG GTAGAACCGG GCAGAAAGGG
    ACGCAAGGCA GTCCTAGCTC CAGAGGGAGC AGGGGAAGAG AAGGCCAGAG GGGAGTCCAG
    GGTGCCTTGG GGGAACCGGG CACACCTGGA CCTCAAGGTG AATCAGGAGC TGAAGGATTG
    CAAGGATCAC AGGGAGCCAG TGGACCTCCG GGCGGGAAAG GAGAGAAGGG AAGCGAGGGA
    CGCAAAGGAC CTCAGGGTGC TCCTGGACCA GTGGGAGAGA AAGGGAGTGT TGGAAGGCCT
    GGTCCTTTGG GGAAAAAGGG AGAACCTGGG ATTCCTGGAG ATCCAGGGCC AGCGGGGCAA
    GTGGGACAGC GAGGAAAGCA GGGTGACTAT GGCATCCCAG GCTATGGTCC TATGGGAGGA
    AAAGGAGTCA AGGGTCCACG AGGATTCCCT GGAGATATGG GCCAAAAGGG TGATACTGGT
    GATCCTGGAA TTCCTGGGGG GCCTGGACCC AAAGGATTTA GGGGATTCAC ACTCACGGTA
    GGCCTGAAAG GTGAAGCGGG CTCTCCTGGA AACCAAGGCC CCCCTGGACG GAGAGGCCCT
    AAAGGAATGG CAGGGAAGCC CATATATTCT CAATGTGATC TAATCCAGTT CACGCGGGAC
    CATAGTCCTT GTTGGAAAGA AAAGTGTCCT GTGTATCCAA CAGAGCTGGT ATTTGCCCTG
    GACCAGTCCA ACGATATCAC AGAGCAGAGA TTTAATGAAA TGAGGGACAT CATCACTTCT
    ATTGTCAATG ACCTTCACAT CAGGGAAAGT AATTGCCCCG AGGGAGCCAG AGTTGTTGTG
    GTTTCCTACA ACTCGGGCAC CGGCTACCTC ATCCATGGGT CTGACTACCG TAGCAAGAAG
    CAGCTTCTTC AGCTTCTCTC CCAAATAAAA TATCAAAGTT CTGGGGAAGT CCGAGACATT
    GGGAACGCGA TGAGGTTTGT GGCTCGGAAC ATCTTTAAGC GGACGCCTGC AGGAGCCAAT
    GTGCAGAGAG TTGCTGTGTT TTTTAGCAAC GGACAAGCTG CGAGCAGGTC ATCTGTCATC
    ACAGCCACCA TGGAGTTCAG TGCCTTGGAT ATCAGTCCTG CAGTCTTCGC TTTTAATGAA
    AAGGTTTACC TCGATGAGGC TTTTGGGTTT GACAACACAG GAACATTTCA GGTGCTTTCT
    GTTCCTCCAA ATGGGGAATA TGATCCATTA GAAAGGCTTC GGCGCTGTAC ACTTTGCTAT
    GATAAATGTT TTCCAAATAC TTGCAAGAAA GAAATTGTCA TACCTGAAAA TTCATACATG
    GATGTCGCCT TCCTCTTAGA CAATTCTAGA AATGTAGCAA GTGATGAGTT TAAGGATATG
    AAAGCCTTGG TGAGCTCAAT GCTTGACAAC TTTGAAATTG CCTCAGACCC TATAGTCTCA
    GACTCTGGTG ATAGGATTGC CTTGTTGAGC TATTCTCCTT GGGATAGGAG AAAGAAGAAT
    GTGGTAAAAA CAGAGTTTGA GTTTACAACT TATAACAGTC AAGCTCTGAT GAAAAGGTAC
    ATTGAGACTA ACCTCCGACA GCTAAATGGG GAAGCCACCA TTGGCCATGC CCTACTGTGG
    GTCGTGGAGA ATCTCTTCCC AGCAACGCCG AAGCTAAGAA AATACAGGGT CATCTTTGTG
    GTCTCCGCTG GAGAGAATCG TGAGAGAAAG GAATTCTTGA AGAAGATGGC TCTGAGGGCC
    AAGTGTCAAG GCTATGTCAT ATTTGTGATT TCCCTGGGTT TGACATCCGA GGAGGACATG
    GAGGAGCTGG CCAGCCACCC GCTTGATCAC CATCTGATAC AGCTGGGGAG AATTCATAAA
    CCAAATCTGG ATTATGTTGT GAAGTTTTTA AAGCCGTTTG TGTACTCAGT GAGACGAGGA
    TTCAATCAGT ACCCACCACC AGTGCTTGAG AACATCTGCC AACTCATTGA TTCAGAAGAC
    GAGGAAAATC AAGGCATCGG TCTCCCATTT ACTCCTGAAC CTTATGAGAT TTCTTCAGGA
    GAGAACATCA TTGGCCAGGA ATTGAGTGCT CTGAGAGATT CATCTTTTGT ATTGGAGGAC
    AATGGAAGCG ACCATTTGGT TTATGTTCCA AGCCAGATGC TTATGCCACA GAAATTAATG
    ACTAAGTATG AAAAAGATTG GAATTCTGAA GCAACTGCAA GTCTCACATC TGATGGTGAA
    GACACAAGAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024852941.1
    CDS206..6997
    Translation

    Target ORF information:

    RefSeq Version XM_024997173.1
    Organism Bos taurus(cattle)
    Definition Bos taurus collagen type VI alpha 5 chain (COL6A5), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024997173.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    ATGAAGATCC TGCTAATTAT ATTTGTTCCT CTCTTCTGGA CTAAAACGCT GTCATATCAG 
    AGTCCAGGGC CAGGCCCTGA GTATGCTGAT GTGGTGTTTC TGATGGACAG CTCCGACCAC
    CTGGGAATTA AGTCCTTCCC GTTTGTGAAG ACCTTCATCA ACAAGATGAT CAGCAGCCTC
    CCCATAGAGG CTGACAAGTA CCACGTGGGC CTGGCCCAGT ACAGCGATGG GCTCCACAGA
    GAGTTCCTGC TCAGCGCCTT CAAGAGCAGG GGCCCCATGC TGAACCACCT CAAGAAGAAC
    TTCGGGTTCC TGGGGGGCTC CCTGCGGATT GGGAAGGCTC TCCAGGAGGT TCACAGGGCC
    TACTTCTCAA GCGGGAGGGA CAGGAAACAG TTTCCCCCGA TCCTGGTGGT CCTGGCTTCC
    GGAGAGTCTG AAGATGCTGT GGAAGAGGCC GCCGAAGCGC TGCGCAAAGA TGGGGTGAGG
    ATCGTCTCTG TGGGGATACA GGGGGCATCT GAGAAGACCC TGAAGGCCAT GGCCACGGGC
    CAGTTCCACT ACCGCCTCCG GACGGTCAGG GACCTCAGCG CATTCTCCCA AAACATGACG
    CAGATTCTCA AGGATGCAGC TCAGTACAAA GATGGAACAG CCTACTCTGA TATAGAAGTC
    CCCTTTCCCA GGGCCTGTAA GAAAGATTCA ATTGCCGACC TCATGTTTCT GGTGGATGAG
    GCCGTTGGGA CCACAGAGAA TCTAAGGCTC CTGCAGAACT TCCTGAAGAA CATCACCTCC
    TCCATGGATG TGCAAGACAG CTGCCTGCGG CTTGGACTTA TGAGCTACAG TGACAGAGTC
    GAGACGGTTT CTCCTCTGAG CTCGAGCACA ACCCAGTCTG AATTTCAGGA GCAAATCCAG
    AAGCTTTCTC TGCGGGCTGG GAAATCCAGT GCTGGAGCTG CCATTGATCA GCTGAGGAGG
    AAAGGCTTCT CAGCAGCACA TGGCAGCAGA CGGGCACAAG GAGTGCCCCA GATAGCAGTC
    CTGGTTACGG ACAGGCCGTC GGACGACAGC GTGCGTGAGG TGGCCCTCGA CCTCCGGCTG
    CAGGACGTAA CCGTGCTTGC CTTGGGCATT GAAGGGGCCA ACAAGACCCA GTTAGAAGAA
    ATAGTGTCTT ACCCTCCGGA GCAGACCATC TCCATGCTGC AGTCCTACGC AGACTTGGAT
    AATTATAGTA AGAAGTTCTT AAAAAAGCTC CAGAATGAAA TATGGTCCCA AGTCTCGGCT
    CGTGCTGAAC AAATGGAACT TGATAAAACT GGCTGTGTGG ACACAAAAGA GGCTGATCTC
    TATTTCCTCA TCGATGGCTC AAGCAGCATT CAGAAAAAAC ACTTTGAGCA AATCAGGGAA
    TTCATGTTGG CGGTGATAAG GATGTTTAGC ATTGGTCCAG ACAAAGTTCG AGTTGGAGCT
    GTGCAGTATT CCCATGAGAA AAAAGTGGAA TTTGATATCA GTGACTACCT TGATGATGTG
    AAATTAAGAA AGGCTGTTTT TAACATCAAG CAACTTGGGG GCGGAACTCG TACTGGGGCA
    GCCCTGGATT TCATGCTGCC ACTGATGAGG GAGGGAAGGA AGCAGAGGAT GAATGAGGTA
    CCCTGTCACC TCATTGTGCT GACCGATGGG ATATCTGGAG ATGCTGTCCT GGAACCTGCT
    GAGAGACTAA GGGCTGAAAA TATCACCATC CACGCGATTG GTATTGGGAA GGCTAACAGA
    ACACAACTGC AACAGATTGC GAGGGAAGAG GAAAGGGTTA ACTTTGGGCA GAACTTTGAT
    GCTTTAAAAA GCATCACGAA TGAAGTTGTT CGCAGTATCT GCACTGAGAA AGGATGTGAA
    AGCATGAAGG CTGACATCAT GTTTCTGGTG GACAGTTCTG GCAGTATAGG ACCTGAAAAT
    TTTGAGAAAA TGAAAACTTT CATGAAAAAC CTGTTAGATA AGATTCAGAT TGGTGAAGAC
    AAAAGCCGAA TTGGTGTAGT TCAATTCAGT CATTATAGTA AGGAAGAATT TCAGCTTGAC
    AAATACTTTA CACAAAAAGA AATTTCTGAT GCAATAGACA GCATGTCCCC CATCAGTGAC
    AATACTTTGA CTGGAAGTGC GATAACCTTT GTGGATCCAT ACTTCACCGA GTCCAAGGGG
    GGCCGCTCAA TGGTAAAAAA GTTTCTCATC CTTATCACTG ATGGAGAAGC CCAGGATGAT
    GTGAGAGACC CCGCAAAAGC TCTTCGGGAC AAAGGTGTGG TCATCTTCTC CGTTGGGGTA
    TACGGGGCCA ACAGGACTCA GCTGGAGGAG ATCAGCGGGG ATGGCAGCCT GATCTTCCAC
    GTTGAGAAAT TCGATGATCT AAAGGCAATA GAGAGTAAAC TCATCTTTCG CGTGTGTGCC
    CTTCATGATT GTAAACGGAT TCAGCAGTTA GATGTCGTGT TCGTGCTGGA CCACTCAGGC
    AGCATCAACC CCGAGGACCA AGAACACATG ATCAACCTCA CCATCCACCT GGTGAAGAAA
    TCAGACGTGG GCCTGGACCG CGTTCGGTTT GGAGCGCTCA GATACTCAGA TGACCCCGAG
    GTTCTTTTCT ACCTCAACAA GTACTCCAGT AAACCAGCCA TCATCGAGCA TCTGAGGAGG
    CGCAGGGATA CGGCGGGAAG AACCTACACC GCCAAGGCTC TGGAGCGCGC GAATGACATG
    TTCACAGAGG AACACGGCAG CCGTATCAAA CAAAATGTGA GGCAGATGCT GATCGTTATC
    ACAGATGGGG TATCTCACGA CAGAGATAAG CTCGGTGAGG CGGCCTCAAA ATTAAGAGCC
    AAAGGCGTCA TCATTTACGC AGTGGGTGTA GGTGCGGCTG ACCAAGCGGA ACTGGAGACC
    ATGGCGGGGG ATAAAAACTA CACTATCCAT GTCAGTGGTT TCGACAAACT GAAAGATATT
    TACCTGCCTG TGCAAGACAG TATGTGTAAC AACTCGCAAG AGGCCTGTGA CATTCCAGAA
    GCTGATGTGA TTTTCTTTTG TGATGGCTCT GACATGGTAT CTGATTCAGA CTTTGTTACC
    ATGACAACTT TCTTGTCAGA CTTAATTGAT AATTTTGACA TTCCATCTGA GAGGATGAAA
    ATTGGGATGG CCCAATTTGG GAGCCGCTAC CAAGAAATCA TCGAGTTACA GAACTCTCTG
    ACTAAAACCG AGTGGAAGTC TCGGATTCAA GCTATCACCA AGAGCAAGGG CCTTCCGCGA
    ATAGACTTGG CCCTGAGAAA AGTGGGCATT ATGTTTGAGC CATCCGCTGG CGGCAGAAGG
    AGCGCTGGTG TCCCTCAGAC TTTGGTGGTC ATCACATCTG GGGGTCCCCG CTATGATGTG
    TCAAAGGCAG TGGAGGCCCT GAGAGAAGCT GGCATTTGTA TTCTGGCTCT GGGCATAGGA
    GATGTTTACA AGGAACAGCT CCTGCCGATA ACGGGCAGTT CTGAAAAAAT CATCACTTTT
    CAAGACTTTG ATAAATTAAA GAATGTGGAT GTGAAAAAAA GAATGATCCG TGAAATCTGC
    AAGAGTTGCG GGAAAACCAA CTGCTTTATG GAGATTGTGG TTGGCTTCGA CATTTCCACT
    CACCTGCCGG GTCAGCCATT GTTCCATGGC CACCCCCGGC TGCAGTCATA CCTCCCAAGC
    ATCTTAGAGG ACATCACCTC CATCAGGGGT GTCAGCTGCG GGGCAGGTGC AGAGGTGCAG
    GTGAGCGTGG CCTTTAAGGT GAACAGTGGC CAGAACGTCC CTGCCGAGTT CCAAATCTAC
    CAGCAAACAA TATTTGACAG CCTCCTGCAA GTCACCGTCA ACGGACCGAC TCACCTGGAT
    GCGCAGTTCC TGCAGTCCAT GTGGGACACC TTTGAGAATG TGTCTACATC CCAAGGACAG
    GTGTTGCTCA TCTTTTCAGA TGGTCTTGGG GGTGAAAGCA AAATGATGCT TGAAGATCAA
    TCGGACAGGC TCAGGGAAGC AGGACTTGAT GCTTTGCTGG TAGTGTCCCT AAATGAAACC
    GCTCACGATG AGTTTTCCAG CTTCGAATTT GGAAAAGGGT TTGACTACAG GACTCACCTG
    ACCATTGGAA TGAAGGAACT GGGCAGTATG CTATCACAGT ATCTGGGGAA CATTGCAGAG
    AGGACGTGTT GCTGTCAGTT TTGCAAATGC CCAGGGATTC CAGGACCTCA GGGGAGCCAA
    GGGCCACAAG CCTCCCAGGG CTCTCCAGGT GTGAAAGGCA GCAGAGGACA CAGGGGAGAG
    GAGGGAGAGC CTGGAGCTCG AGGAGAAATT GGACCCCAAG GAAACAGAGG GAATGCAGGA
    TGTCCAGGGC AGCCAGGTCA AAAGGGAGCC AGAGGATTTT CTGGATATAA GGGAGAACAC
    GGAGATGATG GGATTGATGG ACTGGATGGG GAAGAGGGCT TTCATGGACT TCCTGGGAAA
    AAGGGAGAAA AAGGTGATCC AGGTTCCCAG GGCAGCCCAG GTTCCAGAGG CCTCCCTGGG
    GGATATGGGG ACAAGGGTTT CCCAGGGGAT CCTGGTAATC CAGGACAAAG CAGTACCATC
    AAAGGACAAA AGGGCTCCAA AGGAGAACAA GGAAGACAAG GTAGAACCGG GCAGAAAGGG
    ACGCAAGGCA GTCCTAGCTC CAGAGGGAGC AGGGGAAGAG AAGGCCAGAG GGGAGTCCAG
    GGTGCCTTGG GGGAACCGGG CACACCTGGA CCTCAAGGTG AATCAGGAGC TGAAGGATTG
    CAAGGATCAC AGGGAGCCAG TGGACCTCCG GGCGGGAAAG GAGAGAAGGG AAGCGAGGGA
    CGCAAAGGAC CTCAGGGTGC TCCTGGACCA GTGGGAGAGA AAGGGAGTGT TGGAAGGCCT
    GGTCCTTTGG GGAAAAAGGG AGAACCTGGG ATTCCTGGAG ATCCAGGGCC AGCGGGGCAA
    GTGGGACAGC GAGGAAAGCA GGGTGACTAT GGCATCCCAG GCTATGGTCC TATGGGAGGA
    AAAGGAGTCA AGGGTCCACG AGGATTCCCT GGAGATATGG GCCAAAAGGG TGATACTGGT
    GATCCTGGAA TTCCTGGGGG GCCTGGACCC AAAGGATTTA GGGGATTCAC ACTCACGGTA
    GGCCTGAAAG GTGAAGCGGG CTCTCCTGGA AACCAAGGCC CCCCTGGACG GAGAGGCCCT
    AAAGGAATGG CAGGGAAGCC CATATATTCT CAATGTGATC TAATCCAGTT CACGCGGGAC
    CATAGTCCTT GTTGGAAAGA AAAGTGTCCT GTGTATCCAA CAGAGCTGGT ATTTGCCCTG
    GACCAGTCCA ACGATATCAC AGAGCAGAGA TTTAATGAAA TGAGGGACAT CATCACTTCT
    ATTGTCAATG ACCTTCACAT CAGGGAAAGT AATTGCCCCG AGGGAGCCAG AGTTGTTGTG
    GTTTCCTACA ACTCGGGCAC CGGCTACCTC ATCCATGGGT CTGACTACCG TAGCAAGAAG
    CAGCTTCTTC AGCTTCTCTC CCAAATAAAA TATCAAAGTT CTGGGGAAGT CCGAGACATT
    GGGAACGCGA TGAGGTTTGT GGCTCGGAAC ATCTTTAAGC GGACGCCTGC AGGAGCCAAT
    GTGCAGAGAG TTGCTGTGTT TTTTAGCAAC GGACAAGCTG CGAGCAGGTC ATCTGTCATC
    ACAGCCACCA TGGAGTTCAG TGCCTTGGAT ATCAGTCCTG CAGTCTTCGC TTTTAATGAA
    AAGGTTTACC TCGATGAGGC TTTTGGGTTT GACAACACAG GAACATTTCA GGTGCTTTCT
    GTTCCTCCAA ATGGGGAATA TGATCCATTA GAAAGGCTTC GGCGCTGTAC ACTTTGCTAT
    GATAAATGTT TTCCAAATAC TTGCAAGAAA GAAATTGTCA TACCTGAAAA TTCATACATG
    GATGTCGCCT TCCTCTTAGA CAATTCTAGA AATGTAGCAA GTGATGAGTT TAAGGATATG
    AAAGCCTTGG TGAGCTCAAT GCTTGACAAC TTTGAAATTG CCTCAGACCC TATAGTCTCA
    GACTCTGGTG ATAGGATTGC CTTGTTGAGC TATTCTCCTT GGGATAGGAG AAAGAAGAAT
    GTGGTAAAAA CAGAGTTTGA GTTTACAACT TATAACAGTC AAGCTCTGAT GAAAAGGTAC
    ATTGAGACTA ACCTCCGACA GCTAAATGGG GAAGCCACCA TTGGCCATGC CCTACTGTGG
    GTCGTGGAGA ATCTCTTCCC AGCAACGCCG AAGCTAAGAA AATACAGGGT CATCTTTGTG
    GTCTCCGCTG GAGAGAATCG TGAGAGAAAG GAATTCTTGA AGAAGATGGC TCTGAGGGCC
    AAGTGTCAAG GCTATGTCAT ATTTGTGATT TCCCTGGGTT TGACATCCGA GGAGGACATG
    GAGGAGCTGG CCAGCCACCC GCTTGATCAC CATCTGATAC AGCTGGGGAG AATTCATAAA
    CCAAATCTGG ATTATGTTGT GAAGTTTTTA AAGCCGTTTG TGTACTCAGT GAGACGAGGA
    TTCAATCAGT ACCCACCACC AGTGCTTGAG AACATCTGCC AACTCATTGA TTCAGAAGAC
    GAGGAAAATC AAGGCATCGG TCTCCCATTT ACTCCTGAAC CTTATGAGAT TTCTTCAGGA
    GAGAACATCA TTGGCCAGGA ATTGAGTGCT CTGAGAGATT CATCTTTTGT ATTGGAGGAC
    AATGGAAGCG ACCATTTGGT TTATGTTCCA AGCCAGATGC TTATGCCACA GAAATTAATG
    ACTAAGTATG AAAAAGATTG GAATTCTGAA GCAACTGCAA GTCTCACATC TGATGGTGAA
    GACACAAGAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa87867
    Clone ID Related Accession (Same CDS sequence) XM_015474149.1
    Accession Version XM_015474149.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7536bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product collagen alpha-5(VI) chain isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103827.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    ATGAAGATCC TGCTAATTAT ATTTGTTCCT CTCTTCTGGA CTAAAACGCT GTCATATCAG 
    AGTCCAGGGC CAGGCCCTGA GTATGCTGAT GTGGTGTTTC TGATGGACAG CTCCGACCAC
    CTGGGAATTA AGTCCTTCCC GTTTGTGAAG ACCTTCATCA ACAAGATGAT CAGCAGCCTC
    CCCATAGAGG CTGACAAGTA CCACGTGGGC CTGGCCCAGT ACAGCGATGG GCTCCACAGA
    GAGTTCCTGC TCAGCGCCTT CAAGAGCAGG GGCCCCATGC TGAACCACCT CAAGAAGAAC
    TTCGGGTTCC TGGGGGGCTC CCTGCGGATT GGGAAGGCTC TCCAGGAGGT TCACAGGGCC
    TACTTCTCAA GCGGGAGGGA CAGGAAACAG TTTCCCCCGA TCCTGGTGGT CCTGGCTTCC
    GGAGAGTCTG AAGATGCTGT GGAAGAGGCC GCCGAAGCGC TGCGCAAAGA TGGGGTGAGG
    ATCGTCTCTG TGGGGATACA GGGGGCATCT GAGAAGACCC TGAAGGCCAT GGCCACGGGC
    CAGTTCCACT ACCGCCTCCG GACGGTCAGG GACCTCAGCG CATTCTCCCA AAACATGACG
    CAGATTCTCA AGGATGCAGC TCAGTACAAA GATGGAACAG CCTACTCTGA TATAGAAGTC
    CCCTTTCCCA GGGCCTGTAA GAAAGATTCA ATTGCCGACC TCATGTTTCT GGTGGATGAG
    GCCGTTGGGA CCACAGAGAA TCTAAGGCTC CTGCAGAACT TCCTGAAGAA CATCACCTCC
    TCCATGGATG TGCAAGACAG CTGCCTGCGG CTTGGACTTA TGAGCTACAG TGACAGAGTC
    GAGACGGTTT CTCCTCTGAG CTCGAGCACA ACCCAGTCTG AATTTCAGGA GCAAATCCAG
    AAGCTTTCTC TGCGGGCTGG GAAATCCAGT GCTGGAGCTG CCATTGATCA GCTGAGGAGG
    AAAGGCTTCT CAGCAGCACA TGGCAGCAGA CGGGCACAAG GAGTGCCCCA GATAGCAGTC
    CTGGTTACGG ACAGGCCGTC GGACGACAGC GTGCGTGAGG TGGCCCTCGA CCTCCGGCTG
    CAGGACGTAA CCGTGCTTGC CTTGGGCATT GAAGGGGCCA ACAAGACCCA GTTAGAAGAG
    ATAGTGTCTT ACCCTCCGGA GCAGACCATC TCCATGCTGC AGTCCTATGC AGACTTGGAT
    AATTACAGTA AGAAGTTCTT AAAAAAGCTC CAGAATGAAA TATGGTCCCA AGTCTCGGCT
    CGTGCTGAAC AAATGGAACT TGATAAAACT GGCTGTGTGG ACACAAAAGA GGCTGATCTC
    TATTTCCTCA TCGATGGCTC AAGCAGCATT CAGAAAAAAC ACTTTGAGCA AATCAGGGAA
    TTCATGTTGG CGGTGATAAG GATGTTTAGC ATTGGTCCAG ACAAAGTTCG AGTTGGAGCT
    GTGCAGTATT CCCATGAGAA AAAAGTGGAA TTTGATATCA GTGACTACCT TGATGATGTG
    AAATTAAGAA AGGCTGTTTT TAACATCAAG CAACTTGGGG GCGGAACTCG TACTGGGGCA
    GCCCTGGATT TCATGCTGCC ACTGATGAGG GAGGGAAGGA AGCAGAGGAT GAATGAGGTA
    CCCTGTCACC TCATTGTGCT GACCGATGGG ATATCTGGAG ATGCTGTCCT GGAACCTGCT
    GAGAGACTAA GGGCTGAAAA TATCACCATC CACGCGATTG GTATTGGGAA GGCTAACAGA
    ACACAACTGC AACAGATTGC GAGGGAAGAG GAAAGGGTTA ACTTTGGGCA GAACTTTGAT
    GCTTTAAAAA GCATCACGAA TGAAGTTGTT CGCAGTATCT GCACTGAGAA AGGATGTGAA
    AGCATGAAGG CTGACATCAT GTTTCTGGTG GACAGTTCTG GCAGTATAGG ACCTGAAAAT
    TTTGAGAAAA TGAAAACTTT CATGAAAAAC CTGTTAGATA AGATTCAGAT TGGTGAAGAC
    AAAAGCCGAA TTGGTGTAGT TCAATTCAGT CATTATAGTA AGGAAGAATT TCAGCTTGAC
    AAATACTTTA CACAAAAAGA AATTTCTGAT GCAATAGACA GCATGTCCCC CATCAGTGAC
    AATACTTTGA CTGGAAGTGC GATAACCTTT GTGGATCCAT ACTTCACCGA GTCCAAGGGG
    GGCCGCTCAA TGGTAAAAAA GTTTCTCATC CTTATCACTG ATGGAGAAGC CCAGGATGAT
    GTGAGAGACC CCGCAAAAGC TCTTCGGGAC AAAGGTGTGG TCATCTTCTC CGTTGGGGTA
    TACGGGGCCA ACAGGACTCA GCTGGAGGAG ATCAGCGGGG ATGGCAGCCT GATCTTCCAC
    GTTGAGAAAT TCGATGATCT AAAGGCAATA GAGAGTAAAC TCATCTTTCG CGTGTGTGCC
    CTTCATGATT GTAAACGGAT TCAGCAGTTA GATGTCGTGT TCGTGCTGGA CCACTCAGGC
    AGCATCAACC CCGAGGACCA AGAACACATG ATCAACCTCA CCATCCACCT GGTGAAGAAA
    TCAGACGTGG GCCTGGACCG CGTTCGGTTT GGAGCGCTCA GATACTCAGA TGACCCCGAG
    GTTCTTTTCT ACCTCAACAA GTACTCCAGT AAACCAGCCA TCATCGAGCA TCTGAGGAGG
    CGCAGGGATA CGGCGGGAAG AACCTACACC GCCAAGGCTC TGGAGCGCGC GAATGACATG
    TTCACAGAGG AACACGGCAG CCGTATCAAA CAAAATGTGA GGCAGATGCT GATCGTTATC
    ACAGATGGGG TATCTCACGA CAGAGATAAG CTCGGTGAGG CGGCCTCAAA ATTAAGAGCC
    AAAGGCGTCA TCATTTACGC AGTGGGTGTA GGTGCGGCTG ACCAAGCGGA ACTGGAGACC
    ATGGCGGGGG ATAAAAACTA CACTATCCAT GTCAGTGGTT TCGACAAACT GAAAGATATT
    TACCTGCCTG TGCAAGACAG TATGTGTAAC AACTCGCAAG AGGCCTGTGA CATTCCAGAA
    GCTGATGTGA TTTTCTTTTG TGATGGCTCT GACATGGTAT CTGATTCAGA CTTTGTTACC
    ATGACAACTT TCTTGTCAGA CTTAATTGAT AATTTTGACA TTCCATCTGA GAGGATGAAA
    ATTGGGATGG CCCAATTTGG GAGCCGCTAC CAAGAAATCA TCGAGTTACA GAACTCTCTG
    ACTAAAACCG AGTGGAAGTC TCGGATTCAA GCTATCACCA AGAGCAAGGG CCTTCCGCGA
    ATAGACTTGG CCCTGAGAAA AGTGGGCATT ATGTTTGAGC CATCCGCTGG CGGCAGAAGG
    AGCGCTGGTG TCCCTCAGAC TTTGGTGGTC ATCACATCTG GGGGTCCCCG CTATGATGTG
    TCAAAGGCAG TGGAGGCCCT GAGAGAAGCT GGCATTTGTA TTCTGGCTCT GGGCATAGGA
    GATGTTTACA AGGAACAGCT CCTGCCGATA ACGGGCAGTT CTGAAAAAAT CATCACTTTT
    CAAGACTTTG ATAAATTAAA GAATGTGGAT GTGAAAAAAA GAATGATCCG TGAAATCTGC
    AAGAGTTGCG GGAAAACCAG TAAGTGCACC TTTGTTCCTT TGCTTTTGTT TGATTGCAGC
    TTCCTGGTTG TCCATTTCCC AGGGTGTCTT TGGATGGGCT TTCCTTCTTT CGGGAGTGTG
    ATTCCTTGGG TTTTATCACC ACTTTTTTGC TGCCTAGGAC TTGATGCTTT GCTGGTAGTG
    TCCCTAAATG AAACCGCTCA CGATGAGTTT TCCAGCTTCG AATTTGGAAA AGGGTTTGAC
    TACAGGACTC ACCTGACCAT TGGAATGAAG GAACTGGGCA GTATGCTATC ACAGTATCTG
    GGGAACATTG CAGAGAGGAC GTGTTGCTGT CAGTTTTGCA AATGCCCAGG GATTCCAGGA
    CCTCAGGGGA GCCAAGGGCC ACAAGCCTCC CAGGGCTCTC CAGGTGTGAA AGGCAGCAGA
    GGACACAGGG GAGAGGAGGG AGAGCCTGGA GCTCGAGGAG AAATTGGACC CCAAGGAAAC
    AGAGGGAATG CAGGATGTCC AGGGCAGCCA GGTCAAAAGG GAGCCAGAGG ATTTTCTGGA
    TATAAGGGAG AACACGGAGA TGATGGGATT GATGGACTGG ATGGGGAAGA GGGCTTTCAT
    GGACTTCCTG GGAAAAAGGG AGAAAAAGGT GATCCAGGTT CCCAGGGCAG CCCAGGTTCC
    AGAGGCCTCC CTGGGGGATA TGGGGACAAG GGTTTCCCAG GGGATCCTGG TAATCCAGGA
    CAAAGCAGTA CCATCAAAGG ACAAAAGGGC TCCAAAGGAG AACAAGGAAG ACAAGGTAGA
    ACCGGGCAGA AAGGGACGCA AGGCAGTCCT AGCTCCAGAG GGAGCAGGGG AAGAGAAGGC
    CAGAGGGGAG TCCAGGGTGC CTTGGGGGAA CCGGGCACAC CTGGACCTCA AGGTGAATCA
    GGAGCTGAAG GATTGCAAGG ATCACAGGGA GCCAGTGGAC CTCCGGGCGG GAAAGGAGAG
    AAGGGAAGCG AGGGACGCAA AGGACCTCAG GGTGCTCCTG GACCAGTGGG AGAGAAAGGG
    AGTGTTGGAA GGCCTGGTCC TTTGGGGAAA AAGGGAGAAC CTGGGATTCC TGGAGATCCA
    GGGCCAGCGG GGCAAGTGGG ACAGCGAGGA AAGCAGGGTG ACTATGGCAT CCCAGGCTAT
    GGTCCTATGG GAGGAAAAGG AGTCAAGGGT CCACGAGGAT TCCCTGGAGA TATGGGCCAA
    AAGGGTGATA CTGGTGATCC TGGAATTCCT GGGGGGCCTG GACCCAAAGG ATTTAGGGGA
    TTCACACTCA CGGTAGGCCT GAAAGGTGAA GCGGGCTCTC CTGGAAACCA AGGCCCCCCT
    GGACGGAGAG GCCCTAAAGG AATGGCAGGG AAGCCCATAT ATTCTCAATG TGATCTAATC
    CAGTTCACGC GGGACCATAG TCCTTGTTGG AAAGAAAAGT GTCCTGTGTA TCCAACAGAG
    CTGGTATTTG CCCTGGACCA GTCCAACGAT ATCACAGAGC AGAGATTTAA TGAAATGAGG
    GACATCATCA CTTCTATTGT CAATGACCTT CACATCAGGG AAAGTAATTG CCCCGAGGGA
    GCCAGAGTTG TTGTGGTTTC CTACAACTCG GGCACCGGCT ACCTCATCCA TGGGTCTGAC
    TACCGTAGCA AGAAGCAGCT TCTTCAGCTT CTCTCCCAAA TAAAATATCA AAGTTCTGGG
    GAAGTCCGAG ACATTGGGAA CGCGATGAGG TTTGTGGCTC GGAACATCTT TAAGCGGACG
    CCTGCAGGAG CCAATGTGCA GAGAGTTGCT GTGTTTTTTA GCAACGGACA AGCTGCGAGC
    AGGTCATCTG TCATCACAGC CACCATGGAG TTCAGTGCCT TGGATATCAG TCCTGCAGTC
    TTCGCTTTTA ATGAAAAGGT TTACCTCGAT GAGGCTTTTG GGTTTGACAA CACAGGAACA
    TTTCAGGTGC TTTCTGTTCC TCCAAATGGG GAATATGATC CATTAGAAAG GCTTCGGCGC
    TGTACACTTT GCTATGATAA ATGTTTTCCA AATACTTGCA AGAAAGAAAT TGTCATACCT
    GAAAATTCAT ACATGGATGT CGCCTTCCTC TTAGACAATT CTAGAAATGT AGCAAGTGAT
    GAGTTTAAGG ATATGAAAGC CTTGGTGAGC TCAATGCTTG ACAACTTTGA AATTGCCTCA
    GACCCTATAG TCTCAGACTC TGGTGATAGG ATTGCCTTGT TGAGCTATTC TCCTTGGGAT
    AGGAGAAAGA AGAATGTGGT AAAAACAGAG TTTGAGTTTA CAACTTATAA CAGTCAAGCT
    CTGATGAAAA GGTACATTGA GACTAACCTC CGACAGCTAA ATGGGGAAGC CACCATTGGC
    CATGCCCTAC TGTGGGTCGT GGAGAATCTC TTCCCAGCAA TGCCGAAGCT AAGAAAATAC
    AGGGTCATCT TTGTGGTCTC CGCTGGAGAG AATCGTGAGA GAAAGGAATT CTTGAAGAAG
    ATGGCTCTGA GGGCCAAGTG TCAAGGCTAT GTCATATTTG TGATTTCCCT GGGTTTGACA
    TCCGAGGAGG ACATGGAGGA GCTGGCCAGC CACCCGCTTG ATCACCATCT GATACAGCTG
    GGGAGAATTC ATAAACCAAA TCTGGATTAT GTTGTGAAGT TTTTAAAGCC GTTTGTGTAC
    TCAGTGAGAC GAGGATTCAA TCAGTACCCA CCACCAGTGC TTGAGAACAT CTGCCAACTC
    ATTGATTCAG AAGACGAGGA AAATCAAGGC ATCGGTCTCC CATTTACTCC TGAACCTTAT
    GAGATTTCTT CAGGAGAGAA CATCATTGGC CAGGAATTGA GTGCTCTGAG AGATTCATCT
    TTTGTATTGG AGGACAATGG AAGCGACCAT TTGGTTTATG TTCCAAGCCA GATGCTTATG
    CCACAGAAAT TAATGACTAA GTATGAAAAA GATTGGAATT CTGAAGCAAC TGCAAGTCTC
    ACATCTGGAC ATGAAAACCT TGGCAGAAAA GAAGAACCAG GTCTTACTTA TGAAAGTAGA
    GATGCCTCTC TTCAAGAATA TTACATGGAT GTGACTTTTC TCATAGATGC TTCCAAAAGA
    ATAGGAAGTG ATGAGTTTAA GGAAGTGAAA GCTTTTGTAA CCTCAGTGCT TGATTATTTT
    CATCTGGCCC CAGATCCACT GACCTCCACC TTAGGAGACA GAATAGCAGT CCTGACCTAC
    TCTCCTCCAG GCTATATGCC CAACACAGAA AAATGCCCCG TCTACCTAGA ATTTGATTTG
    GTCACTTATA ACAATATACA CCAAATGAAA CATCATCTCC AAGACTCCCT TCAGCAGCTC
    AATGGAGATG TTTTTATAGG CCACGCCCTT CAGTGGACAC TTGACAATGT CTTTGTAGGA
    ACTCCCAACC TGAGGAGAAA CAAAGTTATC TTTGTTATAT CCGCTGGTGA AACCAACCCT
    TTAGACAGGG AAGTCTTAAG AAATGTATCT CTGAGAGCCA AGTGTCAGGG CTACTCCATA
    TTTGTGTTTT CTTTTGGTCC TCTACACAAT GACAAGGAAT TAGAAGAATT AGCCAGCCAC
    CCACTGGATC ATCACTTAGT CCAGCTTGGC CGAACCCACA AGCCAGATTT GAACTATATC
    ATCAAATTTA TCAAGCCATT TGTTCATTCA ATCAGACGTG GCATCAACAA ATACCCCCCT
    GCAGACATGA AACCCAAATG TGTTAACATC ACCTCTCCCA CTCCAGAGAA TAATGGCATA
    GGAAACACTG TATTCCTTCT TCCTGAGGTA TATGAAATTG AGATGGAGAA CAGTGAGCTT
    TCGGGTGACC CCGATTCCCA GCAGCAGCAT TTCTTTGAAA TAGGGAACAG TCATAGTAAC
    AGTTCTGGTA TCAACACTAA TTTGATCCAG AGGTTATACA TGCTCTTTTC ATCTGGGGAA
    CTGATGATGC AAGACAAGAA AGAGGCACAT TCAGAAGAAA TTACATCTCC AGCAAATGAC
    AGACAAGATG AAAAAGATGG TGAAGACACA AGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015329635.1
    CDS170..7705
    Translation

    Target ORF information:

    RefSeq Version XM_015474149.1
    Organism Bos taurus(cattle)
    Definition Bos taurus collagen type VI alpha 5 (COL6A5), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015474149.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    ATGAAGATCC TGCTAATTAT ATTTGTTCCT CTCTTCTGGA CTAAAACGCT GTCATATCAG 
    AGTCCAGGGC CAGGCCCTGA GTATGCTGAT GTGGTGTTTC TGATGGACAG CTCCGACCAC
    CTGGGAATTA AGTCCTTCCC GTTTGTGAAG ACCTTCATCA ACAAGATGAT CAGCAGCCTC
    CCCATAGAGG CTGACAAGTA CCACGTGGGC CTGGCCCAGT ACAGCGATGG GCTCCACAGA
    GAGTTCCTGC TCAGCGCCTT CAAGAGCAGG GGCCCCATGC TGAACCACCT CAAGAAGAAC
    TTCGGGTTCC TGGGGGGCTC CCTGCGGATT GGGAAGGCTC TCCAGGAGGT TCACAGGGCC
    TACTTCTCAA GCGGGAGGGA CAGGAAACAG TTTCCCCCGA TCCTGGTGGT CCTGGCTTCC
    GGAGAGTCTG AAGATGCTGT GGAAGAGGCC GCCGAAGCGC TGCGCAAAGA TGGGGTGAGG
    ATCGTCTCTG TGGGGATACA GGGGGCATCT GAGAAGACCC TGAAGGCCAT GGCCACGGGC
    CAGTTCCACT ACCGCCTCCG GACGGTCAGG GACCTCAGCG CATTCTCCCA AAACATGACG
    CAGATTCTCA AGGATGCAGC TCAGTACAAA GATGGAACAG CCTACTCTGA TATAGAAGTC
    CCCTTTCCCA GGGCCTGTAA GAAAGATTCA ATTGCCGACC TCATGTTTCT GGTGGATGAG
    GCCGTTGGGA CCACAGAGAA TCTAAGGCTC CTGCAGAACT TCCTGAAGAA CATCACCTCC
    TCCATGGATG TGCAAGACAG CTGCCTGCGG CTTGGACTTA TGAGCTACAG TGACAGAGTC
    GAGACGGTTT CTCCTCTGAG CTCGAGCACA ACCCAGTCTG AATTTCAGGA GCAAATCCAG
    AAGCTTTCTC TGCGGGCTGG GAAATCCAGT GCTGGAGCTG CCATTGATCA GCTGAGGAGG
    AAAGGCTTCT CAGCAGCACA TGGCAGCAGA CGGGCACAAG GAGTGCCCCA GATAGCAGTC
    CTGGTTACGG ACAGGCCGTC GGACGACAGC GTGCGTGAGG TGGCCCTCGA CCTCCGGCTG
    CAGGACGTAA CCGTGCTTGC CTTGGGCATT GAAGGGGCCA ACAAGACCCA GTTAGAAGAG
    ATAGTGTCTT ACCCTCCGGA GCAGACCATC TCCATGCTGC AGTCCTATGC AGACTTGGAT
    AATTACAGTA AGAAGTTCTT AAAAAAGCTC CAGAATGAAA TATGGTCCCA AGTCTCGGCT
    CGTGCTGAAC AAATGGAACT TGATAAAACT GGCTGTGTGG ACACAAAAGA GGCTGATCTC
    TATTTCCTCA TCGATGGCTC AAGCAGCATT CAGAAAAAAC ACTTTGAGCA AATCAGGGAA
    TTCATGTTGG CGGTGATAAG GATGTTTAGC ATTGGTCCAG ACAAAGTTCG AGTTGGAGCT
    GTGCAGTATT CCCATGAGAA AAAAGTGGAA TTTGATATCA GTGACTACCT TGATGATGTG
    AAATTAAGAA AGGCTGTTTT TAACATCAAG CAACTTGGGG GCGGAACTCG TACTGGGGCA
    GCCCTGGATT TCATGCTGCC ACTGATGAGG GAGGGAAGGA AGCAGAGGAT GAATGAGGTA
    CCCTGTCACC TCATTGTGCT GACCGATGGG ATATCTGGAG ATGCTGTCCT GGAACCTGCT
    GAGAGACTAA GGGCTGAAAA TATCACCATC CACGCGATTG GTATTGGGAA GGCTAACAGA
    ACACAACTGC AACAGATTGC GAGGGAAGAG GAAAGGGTTA ACTTTGGGCA GAACTTTGAT
    GCTTTAAAAA GCATCACGAA TGAAGTTGTT CGCAGTATCT GCACTGAGAA AGGATGTGAA
    AGCATGAAGG CTGACATCAT GTTTCTGGTG GACAGTTCTG GCAGTATAGG ACCTGAAAAT
    TTTGAGAAAA TGAAAACTTT CATGAAAAAC CTGTTAGATA AGATTCAGAT TGGTGAAGAC
    AAAAGCCGAA TTGGTGTAGT TCAATTCAGT CATTATAGTA AGGAAGAATT TCAGCTTGAC
    AAATACTTTA CACAAAAAGA AATTTCTGAT GCAATAGACA GCATGTCCCC CATCAGTGAC
    AATACTTTGA CTGGAAGTGC GATAACCTTT GTGGATCCAT ACTTCACCGA GTCCAAGGGG
    GGCCGCTCAA TGGTAAAAAA GTTTCTCATC CTTATCACTG ATGGAGAAGC CCAGGATGAT
    GTGAGAGACC CCGCAAAAGC TCTTCGGGAC AAAGGTGTGG TCATCTTCTC CGTTGGGGTA
    TACGGGGCCA ACAGGACTCA GCTGGAGGAG ATCAGCGGGG ATGGCAGCCT GATCTTCCAC
    GTTGAGAAAT TCGATGATCT AAAGGCAATA GAGAGTAAAC TCATCTTTCG CGTGTGTGCC
    CTTCATGATT GTAAACGGAT TCAGCAGTTA GATGTCGTGT TCGTGCTGGA CCACTCAGGC
    AGCATCAACC CCGAGGACCA AGAACACATG ATCAACCTCA CCATCCACCT GGTGAAGAAA
    TCAGACGTGG GCCTGGACCG CGTTCGGTTT GGAGCGCTCA GATACTCAGA TGACCCCGAG
    GTTCTTTTCT ACCTCAACAA GTACTCCAGT AAACCAGCCA TCATCGAGCA TCTGAGGAGG
    CGCAGGGATA CGGCGGGAAG AACCTACACC GCCAAGGCTC TGGAGCGCGC GAATGACATG
    TTCACAGAGG AACACGGCAG CCGTATCAAA CAAAATGTGA GGCAGATGCT GATCGTTATC
    ACAGATGGGG TATCTCACGA CAGAGATAAG CTCGGTGAGG CGGCCTCAAA ATTAAGAGCC
    AAAGGCGTCA TCATTTACGC AGTGGGTGTA GGTGCGGCTG ACCAAGCGGA ACTGGAGACC
    ATGGCGGGGG ATAAAAACTA CACTATCCAT GTCAGTGGTT TCGACAAACT GAAAGATATT
    TACCTGCCTG TGCAAGACAG TATGTGTAAC AACTCGCAAG AGGCCTGTGA CATTCCAGAA
    GCTGATGTGA TTTTCTTTTG TGATGGCTCT GACATGGTAT CTGATTCAGA CTTTGTTACC
    ATGACAACTT TCTTGTCAGA CTTAATTGAT AATTTTGACA TTCCATCTGA GAGGATGAAA
    ATTGGGATGG CCCAATTTGG GAGCCGCTAC CAAGAAATCA TCGAGTTACA GAACTCTCTG
    ACTAAAACCG AGTGGAAGTC TCGGATTCAA GCTATCACCA AGAGCAAGGG CCTTCCGCGA
    ATAGACTTGG CCCTGAGAAA AGTGGGCATT ATGTTTGAGC CATCCGCTGG CGGCAGAAGG
    AGCGCTGGTG TCCCTCAGAC TTTGGTGGTC ATCACATCTG GGGGTCCCCG CTATGATGTG
    TCAAAGGCAG TGGAGGCCCT GAGAGAAGCT GGCATTTGTA TTCTGGCTCT GGGCATAGGA
    GATGTTTACA AGGAACAGCT CCTGCCGATA ACGGGCAGTT CTGAAAAAAT CATCACTTTT
    CAAGACTTTG ATAAATTAAA GAATGTGGAT GTGAAAAAAA GAATGATCCG TGAAATCTGC
    AAGAGTTGCG GGAAAACCAG TAAGTGCACC TTTGTTCCTT TGCTTTTGTT TGATTGCAGC
    TTCCTGGTTG TCCATTTCCC AGGGTGTCTT TGGATGGGCT TTCCTTCTTT CGGGAGTGTG
    ATTCCTTGGG TTTTATCACC ACTTTTTTGC TGCCTAGGAC TTGATGCTTT GCTGGTAGTG
    TCCCTAAATG AAACCGCTCA CGATGAGTTT TCCAGCTTCG AATTTGGAAA AGGGTTTGAC
    TACAGGACTC ACCTGACCAT TGGAATGAAG GAACTGGGCA GTATGCTATC ACAGTATCTG
    GGGAACATTG CAGAGAGGAC GTGTTGCTGT CAGTTTTGCA AATGCCCAGG GATTCCAGGA
    CCTCAGGGGA GCCAAGGGCC ACAAGCCTCC CAGGGCTCTC CAGGTGTGAA AGGCAGCAGA
    GGACACAGGG GAGAGGAGGG AGAGCCTGGA GCTCGAGGAG AAATTGGACC CCAAGGAAAC
    AGAGGGAATG CAGGATGTCC AGGGCAGCCA GGTCAAAAGG GAGCCAGAGG ATTTTCTGGA
    TATAAGGGAG AACACGGAGA TGATGGGATT GATGGACTGG ATGGGGAAGA GGGCTTTCAT
    GGACTTCCTG GGAAAAAGGG AGAAAAAGGT GATCCAGGTT CCCAGGGCAG CCCAGGTTCC
    AGAGGCCTCC CTGGGGGATA TGGGGACAAG GGTTTCCCAG GGGATCCTGG TAATCCAGGA
    CAAAGCAGTA CCATCAAAGG ACAAAAGGGC TCCAAAGGAG AACAAGGAAG ACAAGGTAGA
    ACCGGGCAGA AAGGGACGCA AGGCAGTCCT AGCTCCAGAG GGAGCAGGGG AAGAGAAGGC
    CAGAGGGGAG TCCAGGGTGC CTTGGGGGAA CCGGGCACAC CTGGACCTCA AGGTGAATCA
    GGAGCTGAAG GATTGCAAGG ATCACAGGGA GCCAGTGGAC CTCCGGGCGG GAAAGGAGAG
    AAGGGAAGCG AGGGACGCAA AGGACCTCAG GGTGCTCCTG GACCAGTGGG AGAGAAAGGG
    AGTGTTGGAA GGCCTGGTCC TTTGGGGAAA AAGGGAGAAC CTGGGATTCC TGGAGATCCA
    GGGCCAGCGG GGCAAGTGGG ACAGCGAGGA AAGCAGGGTG ACTATGGCAT CCCAGGCTAT
    GGTCCTATGG GAGGAAAAGG AGTCAAGGGT CCACGAGGAT TCCCTGGAGA TATGGGCCAA
    AAGGGTGATA CTGGTGATCC TGGAATTCCT GGGGGGCCTG GACCCAAAGG ATTTAGGGGA
    TTCACACTCA CGGTAGGCCT GAAAGGTGAA GCGGGCTCTC CTGGAAACCA AGGCCCCCCT
    GGACGGAGAG GCCCTAAAGG AATGGCAGGG AAGCCCATAT ATTCTCAATG TGATCTAATC
    CAGTTCACGC GGGACCATAG TCCTTGTTGG AAAGAAAAGT GTCCTGTGTA TCCAACAGAG
    CTGGTATTTG CCCTGGACCA GTCCAACGAT ATCACAGAGC AGAGATTTAA TGAAATGAGG
    GACATCATCA CTTCTATTGT CAATGACCTT CACATCAGGG AAAGTAATTG CCCCGAGGGA
    GCCAGAGTTG TTGTGGTTTC CTACAACTCG GGCACCGGCT ACCTCATCCA TGGGTCTGAC
    TACCGTAGCA AGAAGCAGCT TCTTCAGCTT CTCTCCCAAA TAAAATATCA AAGTTCTGGG
    GAAGTCCGAG ACATTGGGAA CGCGATGAGG TTTGTGGCTC GGAACATCTT TAAGCGGACG
    CCTGCAGGAG CCAATGTGCA GAGAGTTGCT GTGTTTTTTA GCAACGGACA AGCTGCGAGC
    AGGTCATCTG TCATCACAGC CACCATGGAG TTCAGTGCCT TGGATATCAG TCCTGCAGTC
    TTCGCTTTTA ATGAAAAGGT TTACCTCGAT GAGGCTTTTG GGTTTGACAA CACAGGAACA
    TTTCAGGTGC TTTCTGTTCC TCCAAATGGG GAATATGATC CATTAGAAAG GCTTCGGCGC
    TGTACACTTT GCTATGATAA ATGTTTTCCA AATACTTGCA AGAAAGAAAT TGTCATACCT
    GAAAATTCAT ACATGGATGT CGCCTTCCTC TTAGACAATT CTAGAAATGT AGCAAGTGAT
    GAGTTTAAGG ATATGAAAGC CTTGGTGAGC TCAATGCTTG ACAACTTTGA AATTGCCTCA
    GACCCTATAG TCTCAGACTC TGGTGATAGG ATTGCCTTGT TGAGCTATTC TCCTTGGGAT
    AGGAGAAAGA AGAATGTGGT AAAAACAGAG TTTGAGTTTA CAACTTATAA CAGTCAAGCT
    CTGATGAAAA GGTACATTGA GACTAACCTC CGACAGCTAA ATGGGGAAGC CACCATTGGC
    CATGCCCTAC TGTGGGTCGT GGAGAATCTC TTCCCAGCAA TGCCGAAGCT AAGAAAATAC
    AGGGTCATCT TTGTGGTCTC CGCTGGAGAG AATCGTGAGA GAAAGGAATT CTTGAAGAAG
    ATGGCTCTGA GGGCCAAGTG TCAAGGCTAT GTCATATTTG TGATTTCCCT GGGTTTGACA
    TCCGAGGAGG ACATGGAGGA GCTGGCCAGC CACCCGCTTG ATCACCATCT GATACAGCTG
    GGGAGAATTC ATAAACCAAA TCTGGATTAT GTTGTGAAGT TTTTAAAGCC GTTTGTGTAC
    TCAGTGAGAC GAGGATTCAA TCAGTACCCA CCACCAGTGC TTGAGAACAT CTGCCAACTC
    ATTGATTCAG AAGACGAGGA AAATCAAGGC ATCGGTCTCC CATTTACTCC TGAACCTTAT
    GAGATTTCTT CAGGAGAGAA CATCATTGGC CAGGAATTGA GTGCTCTGAG AGATTCATCT
    TTTGTATTGG AGGACAATGG AAGCGACCAT TTGGTTTATG TTCCAAGCCA GATGCTTATG
    CCACAGAAAT TAATGACTAA GTATGAAAAA GATTGGAATT CTGAAGCAAC TGCAAGTCTC
    ACATCTGGAC ATGAAAACCT TGGCAGAAAA GAAGAACCAG GTCTTACTTA TGAAAGTAGA
    GATGCCTCTC TTCAAGAATA TTACATGGAT GTGACTTTTC TCATAGATGC TTCCAAAAGA
    ATAGGAAGTG ATGAGTTTAA GGAAGTGAAA GCTTTTGTAA CCTCAGTGCT TGATTATTTT
    CATCTGGCCC CAGATCCACT GACCTCCACC TTAGGAGACA GAATAGCAGT CCTGACCTAC
    TCTCCTCCAG GCTATATGCC CAACACAGAA AAATGCCCCG TCTACCTAGA ATTTGATTTG
    GTCACTTATA ACAATATACA CCAAATGAAA CATCATCTCC AAGACTCCCT TCAGCAGCTC
    AATGGAGATG TTTTTATAGG CCACGCCCTT CAGTGGACAC TTGACAATGT CTTTGTAGGA
    ACTCCCAACC TGAGGAGAAA CAAAGTTATC TTTGTTATAT CCGCTGGTGA AACCAACCCT
    TTAGACAGGG AAGTCTTAAG AAATGTATCT CTGAGAGCCA AGTGTCAGGG CTACTCCATA
    TTTGTGTTTT CTTTTGGTCC TCTACACAAT GACAAGGAAT TAGAAGAATT AGCCAGCCAC
    CCACTGGATC ATCACTTAGT CCAGCTTGGC CGAACCCACA AGCCAGATTT GAACTATATC
    ATCAAATTTA TCAAGCCATT TGTTCATTCA ATCAGACGTG GCATCAACAA ATACCCCCCT
    GCAGACATGA AACCCAAATG TGTTAACATC ACCTCTCCCA CTCCAGAGAA TAATGGCATA
    GGAAACACTG TATTCCTTCT TCCTGAGGTA TATGAAATTG AGATGGAGAA CAGTGAGCTT
    TCGGGTGACC CCGATTCCCA GCAGCAGCAT TTCTTTGAAA TAGGGAACAG TCATAGTAAC
    AGTTCTGGTA TCAACACTAA TTTGATCCAG AGGTTATACA TGCTCTTTTC ATCTGGGGAA
    CTGATGATGC AAGACAAGAA AGAGGCACAT TCAGAAGAAA TTACATCTCC AGCAAATGAC
    AGACAAGATG AAAAAGATGG TGAAGACACA AGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa89499
    Clone ID Related Accession (Same CDS sequence) XM_015462216.1
    Accession Version XM_015462216.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7833bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product LOW QUALITY PROTEIN: collagen alpha-5(VI) chain isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644567.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 4 indels ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    ATGAAGATCC TGCTAATTAT ATTTGTTCCT CTCTTCTGGA CTAAAACGCT GTCATATCAG 
    AGTCCAGGGC CAGGCCCTGA GTATGCTGAT GTGGTGTTTC TGATGGACAG CTCCGACCAC
    CTGGGAATTA AGTCCTTCCC GTTTGTGAAG ACCTTCATCA ACAAGATGAT CAGCAGCCTC
    CCCATAGAGG CTGACAAGTA CCACGTGGGC CTGGCCCAGT ACAGCGATGG GCTCCACAGA
    GAGTTCCTGC TCAGCGCCTT CAAGAGCAGG GGCCCCATGC TGAACCACCT CAAGAAGAAC
    TTCGGGTTCC TGGGGGGCTC CCTGCGGATT GGGAAGGCTC TCCAGGAGGT TCACAGGGCC
    TACTTCTCAA GCGGGAGGGA CAGGAAACAG TTTCCCCCGA TCCTGGTGGT CCTGGCTTCC
    GGAGAGTCTG AAGATGCTGT GGAAGAGGCC GCCGAAGCGC TGCGCAAAGA TGGGGTGAGG
    ATCGTCTCTG TGGGGATACA GGGGGCATCT GAGAAGACCC TGAAGGCCAT GGCCACGGGC
    CAGTTCCACT ACCGCCTCCG GACGGTCAGG GACCTCAGCG CATTCTCCCA AAACATGACG
    CAGATTCTCA AGGATGCAGC TCAGTACAAA GATGGAACAG CCTACTCTGA TATAGAAGTC
    CCCTTTCCCA GGGCCTGTAA GAAAGATTCA ATTGCCGACC TCATGTTTCT GGTGGATGAG
    GCCGTTGGGA CCACAGAGAA TCTAAGGCTC CTGCAGAACT TCCTGAAGAA CATCACCTCC
    TCCATGGATG TGCAAGACAG CTGCCTGCGG CTTGGACTTA TGAGCTACAG TGACAGAGTC
    GAGACGGTTT CTCCTCTGAG CTCGAGCACA ACCCAGTCTG AATTTCAGGA GCAAATCCAG
    AAGCTTTCTC TGCGGGCTGG GAAATCCAGT GCTGGAGCTG CCATTGATCA GCTGAGGAGG
    AAAGGCTTCT CAGCAGCACA TGGCAGCAGA CGGGCACAAG GAGTGCCCCA GATAGCAGTC
    CTGGTTACGG ACAGGCCGTC GGACGACAGC GTGCGTGAGG TGGCCCTCGA CCTCCGGCTG
    CAGGACGTAA CCGTGCTTGC CTTGGGCATT GAAGGGGCCA ACAAGACCCA GTTAGAAGAG
    ATAGTGTCTT ACCCTCCGGA GCAGACCATC TCCATGCTGC AGTCCTATGC AGACTTGGAT
    AATTACAGTA AGAAGTTCTT AAAAAAGCTC CAGAATGAAA TATGGTCCCA AGTCTCGGCT
    CGTGCTGAAC AAATGGAACT TGATAAAACT GGCTGTGTGG ACACAAAAGA GGCTGATCTC
    TATTTCCTCA TCGATGGCTC AAGCAGCATT CAGAAAAAAC ACTTTGAGCA AATCAGGGAA
    TTCATGTTGG CGGTGATAAG GATGTTTAGC ATTGGTCCAG ACAAAGTTCG AGTTGGAGCT
    GTGCAGTATT CCCATGAGAA AAAAGTGGAA TTTGATATCA GTGACTACCT TGATGATGTG
    AAATTAAGAA AGGCTGTTTT TAACATCAAG CAACTTGGGG GCGGAACTCG TACTGGGGCA
    GCCCTGGATT TCATGCTGCC ACTGATGAGG GAGGGAAGGA AGCAGAGGAT GAATGAGGTA
    CCCTGTCACC TCATTGTGCT GACCGATGGG ATATCTGGAG ATGCTGTCCT GGAACCTGCT
    GAGAGACTAA GGGCTGAAAA TATCACCATC CACGCGATTG GTATTGGGAA GGCTAACAGA
    ACACAACTGC AACAGATTGC GAGGGAAGAG GAAAGGGTTA ACTTTGGGCA GAACTTTGAT
    GCTTTAAAAA GCATCACGAA TGAAGTTGTT CGCAGTATCT GCACTGAGAA AGGATGTGAA
    AGCATGAAGG CTGACATCAT GTTTCTGGTG GACAGTTCTG GCAGTATAGG ACCTGAAAAT
    TTTGAGAAAA TGAAAACTTT CATGAAAAAC CTGTTAGATA AGATTCAGAT TGGTGAAGAC
    AAAAGCCGAA TTGGTGTAGT TCAATTCAGT CATTATAGTA AGGAAGAATT TCAGCTTGAC
    AAATACTTTA CACAAAAAGA AATTTCTGAT GCAATAGACA GCATGTCCCC CATCAGTGAC
    AATACTTTGA CTGGAAGTGC GATAACCTTT GTGGATCCAT ACTTCACCGA GTCCAAGGGG
    GGCCGCTCAA TGGTAAAAAA GTTTCTCATC CTTATCACTG ATGGAGAAGC CCAGGATGAT
    GTGAGAGACC CCGCAAAAGC TCTTCGGGAC AAAGGTGTGG TCATCTTCTC CGTTGGGGTA
    TACGGGGCCA ACAGGACTCA GCTGGAGGAG ATCAGCGGGG ATGGCAGCCT GATCTTCCAC
    GTTGAGAAAT TCGATGATCT AAAGGCAATA GAGAGTAAAC TCATCTTTCG CGTGTGTGCC
    CTTCATGATT GTAAACGGAT TCAGCAGTTA GATGTCGTGT TCGTGCTGGA CCACTCAGGC
    AGCATCAACC CCGAGGACCA AGAACACATG ATCAACCTCA CCATCCACCT GGTGAAGAAA
    TCAGACGTGG GCCTGGACCG CGTTCGGTTT GGAGCGCTCA GATACTCAGA TGACCCCGAG
    GTTCTTTTCT ACCTCAACAA GTACTCCAGT AAACCAGCCA TCATCGAGCA TCTGAGGAGG
    CGCAGGGATA CGGCGGGAAG AACCTACACC GCCAAGGCTC TGGAGCGCGC GAATGACATG
    TTCACAGAGG AACACGGCAG CCGTATCAAA CAAAATGTGA GGCAGATGCT GATCGTTATC
    ACAGATGGGG TATCTCACGA CAGAGATAAG CTCGGTGAGG CGGCCTCAAA ATTAAGAGCC
    AAAGGCGTCA TCATTTACGC AGTGGGTGTA GGTGCGGCTG ACCAAGCGGA ACTGGAGACC
    ATGGCGGGGG ATAAAAACTA CACTATCCAT GTCAGTGGTT TCGACAAACT GAAAGATATT
    TACCTGCCTG TGCAAGACAG TATGTGTAAC AACTCGCAAG AGGCCTGTGA CATTCCAGAA
    GCTGATGTGA TTTTCTTTTG TGATGGCTCT GACATGGTAT CTGATTCAGA CTTTGTTACC
    ATGACAACTT TCTTGTCAGA CTTAATTGAT AATTTTGACA TTCCATCTGA GAGGATGAAA
    ATTGGGATGG CCCAATTTGG GAGCCGCTAC CAAGAAATCA TCGAGTTACA GAACTCTCTG
    ACTAAAACCG AGTGGAAGTC TCGGATTCAA GCTATCACCA AGAGCAAGGG CCTTCCGCGA
    ATAGACTTGG CCCTGAGAAA AGTGGGCATT ATGTTTGAGC CATCCGCTGG CGGCAGAAGG
    AGCGCTGGTG TCCCTCAGAC TTTGGTGGTC ATCACATCTG GGGGTCCCCG CTATGATGTG
    TCAAAGGCAG TGGAGGCCCT GAGAGAAGCT GGCATTTGTA TTCTGGCTCT GGGCATAGGA
    GATGTTTACA AGGAACAGCT CCTGCCGATA ACGGGCAGTT CTGAAAAAAT CATCACTTTT
    CAAGACTTTG ATAAATTAAA GAATGTGGAT GTGAAAAAAA GAATGATCCG TGAAATCTGC
    AAGAGTTGCG GGAAAACCAA CTGCTTTATG GAGATTGTGG TTGGCTTCGA CATTTCCACT
    CACCTGNCGG GTCAGCCATT GTTCCATGGC CACCCCCGGC TGCAGTCATA CCTCCCAAGC
    ATCTTAGAGG ACATCACCTC CATCAGGGGT GTCAGCTGCG GGNCAGGTGC AGAGGTGCAG
    GTGAGGGTGG CCTTTAAGGT GAACAGTGGC CAGAACGTCC CTGCCGAGTT CCAAATCTAC
    CAGCAAACAA TATTTGACAG CCTCCTGCAA GTCNACGTCA ACGGACCGAC TCACCTGGAT
    GCGCAGTTCC TGCAGTCCAT GTGGNACACC TTTGAGAATG TGTCTACATC CCAAGGACAG
    GTAACCAAGT CACTTTCTAG CTCCATCTCA TGTCCGTTCG TTAGTTTCTC CCTCCCAGTT
    ATACGTTTTC AATCGGACAG GCTCAGGGAA GCAGGACTTG ATGCTTTGCT GGTAGTGTCC
    CTAAATGAAA CCGCTCACGA TGAGTTTTCC AGCTTCGAAT TTGGAAAAGG GTTTGACTAC
    AGGACTCACC TGACCATTGG AATGAAGGAA CTGGGCAGTA TGCTATCACA GTATCTGGGG
    AACATTGCAG AGAGGACGTG TTGCTGTCAG TTTTGCAAAT GCCCAGGGAT TCCAGGACCT
    CAGGGGAGCC AAGGGCCACA AGCCTCCCAG GGCTCTCCAG GTGTGAAAGG CAGCAGAGGA
    CACAGGGGAG AGGAGGGAGA GCCTGGAGCT CGAGGAGAAA TTGGACCCCA AGGAAACAGA
    GGGAATGCAG GATGTCCAGG GCAGCCAGGT CAAAAGGGAG CCAGAGGATT TTCTGGATAT
    AAGGGAGAAC ACGGAGATGA TGGGATTGAT GGACTGGATG GGGAAGAGGG CTTTCATGGA
    CTTCCTGGGA AAAAGGGAGA AAAAGGTGAT CCAGGTTCCC AGGGCAGCCC AGGTTCCAGA
    GGCCTCCCTG GGGGATATGG GGACAAGGGT TTCCCAGGGG ATCCTGGTAA TCCAGGACAA
    AGCAGTACCA TCAAAGGACA AAAGGGCTCC AAAGGAGAAC AAGGAAGACA AGGTAGAACC
    GGGCAGAAAG GGACGCAAGG CAGTCCTAGC TCCAGAGGGA GCAGGGGAAG AGAAGGCCAG
    AGGGGAGTCC AGGGTGCCTT GGGGGAACCG GGCACACCTG GACCTCAAGG TGAATCAGGA
    GCTGAAGGAT TGCAAGGATC ACAGGGAGCC AGTGGACCTC CGGGCGGGAA AGGAGAGAAG
    GGAAGCGAGG GACGCAAAGG ACCTCAGGGT GCTCCTGGAC CAGTGGGAGA GAAAGGGAGT
    GTTGGAAGGC CTGGTCCTTT GGGGAAAAAG GGAGAACCTG GGATTCCTGG AGATCCAGGG
    CCAGCGGGGC AAGTGGGACA GCGAGGAAAG CAGGGTGACT ATGGCATCCC AGGCTATGGT
    CCTATGGGAG GAAAAGGAGT CAAGGGTCCA CGAGGATTCC CTGGAGATAT GGGCCAAAAG
    GGTGATACTG GTGATCCTGG AATTCCTGGG GGGCCTGGAC CCAAAGGATT TAGGGGATTC
    ACACTCACGG TAGGCCTGAA AGGTGAAGCG GGCTCTCCTG GAAACCAAGG CCCCCCTGGA
    CGGAGAGGCC CTAAAGGAAT GGCAGGGAAG CCCATATATT CTCAATGTGA TCTAATCCAG
    TTCACGCGGG ACCATAGTCC TTGTTGGAAA GAAAAGTGTC CTGTGTATCC AACAGAGCTG
    GTATTTGCCC TGGACCAGTC CAACGATATC ACAGAGCAGA GATTTAATGA AATGAGGGAC
    ATCATCACTT CTATTGTCAA TGACCTTCAC ATCAGGGAAA GTAATTGCCC CGAGGGAGCC
    AGAGTTGTTG TGGTTTCCTA CAACTCGGGC ACCGGCTACC TCATCCATGG GTCTGACTAC
    CGTAGCAAGA AGCAGCTTCT TCAGCTTCTC TCCCAAATAA AATATCAAAG TTCTGGGGAA
    GTCCGAGACA TTGGGAACGC GATGAGGTTT GTGGCTCGGA ACATCTTTAA GCGGACGCCT
    GCAGGAGCCA ATGTGCAGAG AGTTGCTGTG TTTTTTAGCA ACGGACAAGC TGCGAGCAGG
    TCATCTGTCA TCACAGCCAC CATGGAGTTC AGTGCCTTGG ATATCAGTCC TGCAGTCTTC
    GCTTTTAATG AAAAGGTTTA CCTCGATGAG GCTTTTGGGT TTGACAACAC AGGAACATTT
    CAGGTGCTTT CTGTTCCTCC AAATGGGGAA TATGATCCAT TAGAAAGGCT TCGGCGCTGT
    ACACTTTGCT ATGATAAATG TTTTCCAAAT ACTTGCAAGA AAGAAATTGT CATACCTGAA
    AATTCATACA TGGATGTCGC CTTCCTCTTA GACAATTCTA GAAATGTAGC AAGTGATGAG
    TTTAAGGATA TGAAAGCCTT GGTGAGCTCA ATGCTTGACA ACTTTGAAAT TGCCTCAGAC
    CCTATAGTCT CAGACTCTGG TGATAGGATT GCCTTGTTGA GCTATTCTCC TTGGGATAGG
    AGAAAGAAGA ATGTGGTAAA AACAGAGTTT GAGTTTACAA CTTATAACAG TCAAGCTCTG
    ATGAAAAGGT ACATTGAGAC TAACCTCCGA CAGCTAAATG GGGAAGCCAC CATTGGCCAT
    GCCCTACTGT GGGTCGTGGA GAATCTCTTC CCAGCAATGC CGAAGCTAAG AAAATACAGG
    GTCATCTTTG TGGTCTCCGC TGGAGAGAAT CGTGAGAGAA AGGAATTCTT GAAGAAGATG
    GCTCTGAGGG CCAAGTGTCA AGGCTATGTC ATATTTGTGA TTTCCCTGGG TTTGACATCC
    GAGGAGGACA TGGAGGAGCT GGCCAGCCAC CCGCTTGATC ACCATCTGAT ACAGCTGGGG
    AGAATTCATA AACCAAATCT GGATTATGTT GTGAAGTTTT TAAAGCCGTT TGTGTACTCA
    GTGAGACGAG GATTCAATCA GTACCCACCA CCAGTGCTTG AGAACATCTG CCAACTCATT
    GATTCAGAAG ACGAGGAAAA TCAAGGCATC GGTCTCCCAT TTACTCCTGA ACCTTATGAG
    ATTTCTTCAG GAGAGAACAT CATTGGCCAG GAATTGAGTG CTCTGAGAGA TTCATCTTTT
    GTATTGGAGG ACAATGGAAG CGACCATTTG GTTTATGTTC CAAGCCAGAT GCTTATGCCA
    CAGAAATTAA TGACTAAGTA TGAAAAAGAT TGGAATTCTG AAGCAACTGC AAGTCTCACA
    TCTGGACATG AAAACCTTGG CAGAAAAGAA GAACCAGGTC TTACTTATGA AAGTAGAGAT
    GCCTCTCTTC AAGAATATTA CATGGATGTG ACTTTTCTCA TAGATGCTTC CAAAAGAATA
    GGAAGTGATG AGTTTAAGGA AGTGAAAGCT TTTGTAACCT CAGTGCTTGA TTATTTTCAT
    CTGGCCCCAG ATCCACTGAC CTCCACCTTA GGAGACAGAA TAGCAGTCCT GACCTACTCT
    CCTCCAGGCT ATATGCCCAA CACAGAAAAA TGCCCCGTCT ACCTAGAATT TGATTTGGTC
    ACTTATAACA ATATACACCA AATGAAACAT CATCTCCAAG ACTCCCTTCA GCAGCTCAAT
    GGAGATGTTT TTATAGGCCA CGCCCTTCAG TGGACACTTG ACAATGTCTT TGTAGGAACT
    CCCAACCTGA GGAGAAACAA AGTTATCTTT GTTATATCCG CTGGTGAAAC CAACCCTTTA
    GACAGGGAAG TCTTAAGAAA TGTATCTCTG AGAGCCAAGT GTCAGGGCTA CTCCATATTT
    GTGTTTTCTT TTGGTCCTCT ACACAATGAC AAGGAATTAG AAGAATTAGC CAGCCACCCA
    CTGGATCATC ACTTAGTCCA GCTTGGCCGA ACCCACAAGC CAGATTTGAA CTATATCATC
    AAATTTATCA AGCCATTTGT TCATTCAATC AGACGTGGCA TCAACAAATA CCCCCCTGCA
    GACATGAAAC CCAAATGTGT TAACATCACC TCTCCCACTC CAGAGAATAA TGGCATAGGA
    AACACTGTAT TCCTTCTTCC TGAGGTATAT GAAATTGAGA TGGAGAACAG TGAGCTTTCG
    GGTGACCCCG ATTCCCAGCA GCAGCATTTC TTTGAAATAG GGAACAGTCA TAGTAACAGT
    TCTGGTATCA ACACTAATTT GATCCAGAGG TTATACATGC TCTTTTCATC TGGGGAACTG
    ATGATGCAAG ACAAGAAAGA GGCACATTCA GAAGAAATTA CATCTCCAGC AAATGACAGA
    CAAGATGAAA AAGATGGTGA AGACACAAGA TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015317702.1
    CDS171..8003
    Translation

    Target ORF information:

    RefSeq Version XM_015462216.1
    Organism Bos taurus(cattle)
    Definition Bos taurus collagen type VI alpha 5 (COL6A5), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015462216.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    ATGAAGATCC TGCTAATTAT ATTTGTTCCT CTCTTCTGGA CTAAAACGCT GTCATATCAG 
    AGTCCAGGGC CAGGCCCTGA GTATGCTGAT GTGGTGTTTC TGATGGACAG CTCCGACCAC
    CTGGGAATTA AGTCCTTCCC GTTTGTGAAG ACCTTCATCA ACAAGATGAT CAGCAGCCTC
    CCCATAGAGG CTGACAAGTA CCACGTGGGC CTGGCCCAGT ACAGCGATGG GCTCCACAGA
    GAGTTCCTGC TCAGCGCCTT CAAGAGCAGG GGCCCCATGC TGAACCACCT CAAGAAGAAC
    TTCGGGTTCC TGGGGGGCTC CCTGCGGATT GGGAAGGCTC TCCAGGAGGT TCACAGGGCC
    TACTTCTCAA GCGGGAGGGA CAGGAAACAG TTTCCCCCGA TCCTGGTGGT CCTGGCTTCC
    GGAGAGTCTG AAGATGCTGT GGAAGAGGCC GCCGAAGCGC TGCGCAAAGA TGGGGTGAGG
    ATCGTCTCTG TGGGGATACA GGGGGCATCT GAGAAGACCC TGAAGGCCAT GGCCACGGGC
    CAGTTCCACT ACCGCCTCCG GACGGTCAGG GACCTCAGCG CATTCTCCCA AAACATGACG
    CAGATTCTCA AGGATGCAGC TCAGTACAAA GATGGAACAG CCTACTCTGA TATAGAAGTC
    CCCTTTCCCA GGGCCTGTAA GAAAGATTCA ATTGCCGACC TCATGTTTCT GGTGGATGAG
    GCCGTTGGGA CCACAGAGAA TCTAAGGCTC CTGCAGAACT TCCTGAAGAA CATCACCTCC
    TCCATGGATG TGCAAGACAG CTGCCTGCGG CTTGGACTTA TGAGCTACAG TGACAGAGTC
    GAGACGGTTT CTCCTCTGAG CTCGAGCACA ACCCAGTCTG AATTTCAGGA GCAAATCCAG
    AAGCTTTCTC TGCGGGCTGG GAAATCCAGT GCTGGAGCTG CCATTGATCA GCTGAGGAGG
    AAAGGCTTCT CAGCAGCACA TGGCAGCAGA CGGGCACAAG GAGTGCCCCA GATAGCAGTC
    CTGGTTACGG ACAGGCCGTC GGACGACAGC GTGCGTGAGG TGGCCCTCGA CCTCCGGCTG
    CAGGACGTAA CCGTGCTTGC CTTGGGCATT GAAGGGGCCA ACAAGACCCA GTTAGAAGAG
    ATAGTGTCTT ACCCTCCGGA GCAGACCATC TCCATGCTGC AGTCCTATGC AGACTTGGAT
    AATTACAGTA AGAAGTTCTT AAAAAAGCTC CAGAATGAAA TATGGTCCCA AGTCTCGGCT
    CGTGCTGAAC AAATGGAACT TGATAAAACT GGCTGTGTGG ACACAAAAGA GGCTGATCTC
    TATTTCCTCA TCGATGGCTC AAGCAGCATT CAGAAAAAAC ACTTTGAGCA AATCAGGGAA
    TTCATGTTGG CGGTGATAAG GATGTTTAGC ATTGGTCCAG ACAAAGTTCG AGTTGGAGCT
    GTGCAGTATT CCCATGAGAA AAAAGTGGAA TTTGATATCA GTGACTACCT TGATGATGTG
    AAATTAAGAA AGGCTGTTTT TAACATCAAG CAACTTGGGG GCGGAACTCG TACTGGGGCA
    GCCCTGGATT TCATGCTGCC ACTGATGAGG GAGGGAAGGA AGCAGAGGAT GAATGAGGTA
    CCCTGTCACC TCATTGTGCT GACCGATGGG ATATCTGGAG ATGCTGTCCT GGAACCTGCT
    GAGAGACTAA GGGCTGAAAA TATCACCATC CACGCGATTG GTATTGGGAA GGCTAACAGA
    ACACAACTGC AACAGATTGC GAGGGAAGAG GAAAGGGTTA ACTTTGGGCA GAACTTTGAT
    GCTTTAAAAA GCATCACGAA TGAAGTTGTT CGCAGTATCT GCACTGAGAA AGGATGTGAA
    AGCATGAAGG CTGACATCAT GTTTCTGGTG GACAGTTCTG GCAGTATAGG ACCTGAAAAT
    TTTGAGAAAA TGAAAACTTT CATGAAAAAC CTGTTAGATA AGATTCAGAT TGGTGAAGAC
    AAAAGCCGAA TTGGTGTAGT TCAATTCAGT CATTATAGTA AGGAAGAATT TCAGCTTGAC
    AAATACTTTA CACAAAAAGA AATTTCTGAT GCAATAGACA GCATGTCCCC CATCAGTGAC
    AATACTTTGA CTGGAAGTGC GATAACCTTT GTGGATCCAT ACTTCACCGA GTCCAAGGGG
    GGCCGCTCAA TGGTAAAAAA GTTTCTCATC CTTATCACTG ATGGAGAAGC CCAGGATGAT
    GTGAGAGACC CCGCAAAAGC TCTTCGGGAC AAAGGTGTGG TCATCTTCTC CGTTGGGGTA
    TACGGGGCCA ACAGGACTCA GCTGGAGGAG ATCAGCGGGG ATGGCAGCCT GATCTTCCAC
    GTTGAGAAAT TCGATGATCT AAAGGCAATA GAGAGTAAAC TCATCTTTCG CGTGTGTGCC
    CTTCATGATT GTAAACGGAT TCAGCAGTTA GATGTCGTGT TCGTGCTGGA CCACTCAGGC
    AGCATCAACC CCGAGGACCA AGAACACATG ATCAACCTCA CCATCCACCT GGTGAAGAAA
    TCAGACGTGG GCCTGGACCG CGTTCGGTTT GGAGCGCTCA GATACTCAGA TGACCCCGAG
    GTTCTTTTCT ACCTCAACAA GTACTCCAGT AAACCAGCCA TCATCGAGCA TCTGAGGAGG
    CGCAGGGATA CGGCGGGAAG AACCTACACC GCCAAGGCTC TGGAGCGCGC GAATGACATG
    TTCACAGAGG AACACGGCAG CCGTATCAAA CAAAATGTGA GGCAGATGCT GATCGTTATC
    ACAGATGGGG TATCTCACGA CAGAGATAAG CTCGGTGAGG CGGCCTCAAA ATTAAGAGCC
    AAAGGCGTCA TCATTTACGC AGTGGGTGTA GGTGCGGCTG ACCAAGCGGA ACTGGAGACC
    ATGGCGGGGG ATAAAAACTA CACTATCCAT GTCAGTGGTT TCGACAAACT GAAAGATATT
    TACCTGCCTG TGCAAGACAG TATGTGTAAC AACTCGCAAG AGGCCTGTGA CATTCCAGAA
    GCTGATGTGA TTTTCTTTTG TGATGGCTCT GACATGGTAT CTGATTCAGA CTTTGTTACC
    ATGACAACTT TCTTGTCAGA CTTAATTGAT AATTTTGACA TTCCATCTGA GAGGATGAAA
    ATTGGGATGG CCCAATTTGG GAGCCGCTAC CAAGAAATCA TCGAGTTACA GAACTCTCTG
    ACTAAAACCG AGTGGAAGTC TCGGATTCAA GCTATCACCA AGAGCAAGGG CCTTCCGCGA
    ATAGACTTGG CCCTGAGAAA AGTGGGCATT ATGTTTGAGC CATCCGCTGG CGGCAGAAGG
    AGCGCTGGTG TCCCTCAGAC TTTGGTGGTC ATCACATCTG GGGGTCCCCG CTATGATGTG
    TCAAAGGCAG TGGAGGCCCT GAGAGAAGCT GGCATTTGTA TTCTGGCTCT GGGCATAGGA
    GATGTTTACA AGGAACAGCT CCTGCCGATA ACGGGCAGTT CTGAAAAAAT CATCACTTTT
    CAAGACTTTG ATAAATTAAA GAATGTGGAT GTGAAAAAAA GAATGATCCG TGAAATCTGC
    AAGAGTTGCG GGAAAACCAA CTGCTTTATG GAGATTGTGG TTGGCTTCGA CATTTCCACT
    CACCTGNCGG GTCAGCCATT GTTCCATGGC CACCCCCGGC TGCAGTCATA CCTCCCAAGC
    ATCTTAGAGG ACATCACCTC CATCAGGGGT GTCAGCTGCG GGNCAGGTGC AGAGGTGCAG
    GTGAGGGTGG CCTTTAAGGT GAACAGTGGC CAGAACGTCC CTGCCGAGTT CCAAATCTAC
    CAGCAAACAA TATTTGACAG CCTCCTGCAA GTCNACGTCA ACGGACCGAC TCACCTGGAT
    GCGCAGTTCC TGCAGTCCAT GTGGNACACC TTTGAGAATG TGTCTACATC CCAAGGACAG
    GTAACCAAGT CACTTTCTAG CTCCATCTCA TGTCCGTTCG TTAGTTTCTC CCTCCCAGTT
    ATACGTTTTC AATCGGACAG GCTCAGGGAA GCAGGACTTG ATGCTTTGCT GGTAGTGTCC
    CTAAATGAAA CCGCTCACGA TGAGTTTTCC AGCTTCGAAT TTGGAAAAGG GTTTGACTAC
    AGGACTCACC TGACCATTGG AATGAAGGAA CTGGGCAGTA TGCTATCACA GTATCTGGGG
    AACATTGCAG AGAGGACGTG TTGCTGTCAG TTTTGCAAAT GCCCAGGGAT TCCAGGACCT
    CAGGGGAGCC AAGGGCCACA AGCCTCCCAG GGCTCTCCAG GTGTGAAAGG CAGCAGAGGA
    CACAGGGGAG AGGAGGGAGA GCCTGGAGCT CGAGGAGAAA TTGGACCCCA AGGAAACAGA
    GGGAATGCAG GATGTCCAGG GCAGCCAGGT CAAAAGGGAG CCAGAGGATT TTCTGGATAT
    AAGGGAGAAC ACGGAGATGA TGGGATTGAT GGACTGGATG GGGAAGAGGG CTTTCATGGA
    CTTCCTGGGA AAAAGGGAGA AAAAGGTGAT CCAGGTTCCC AGGGCAGCCC AGGTTCCAGA
    GGCCTCCCTG GGGGATATGG GGACAAGGGT TTCCCAGGGG ATCCTGGTAA TCCAGGACAA
    AGCAGTACCA TCAAAGGACA AAAGGGCTCC AAAGGAGAAC AAGGAAGACA AGGTAGAACC
    GGGCAGAAAG GGACGCAAGG CAGTCCTAGC TCCAGAGGGA GCAGGGGAAG AGAAGGCCAG
    AGGGGAGTCC AGGGTGCCTT GGGGGAACCG GGCACACCTG GACCTCAAGG TGAATCAGGA
    GCTGAAGGAT TGCAAGGATC ACAGGGAGCC AGTGGACCTC CGGGCGGGAA AGGAGAGAAG
    GGAAGCGAGG GACGCAAAGG ACCTCAGGGT GCTCCTGGAC CAGTGGGAGA GAAAGGGAGT
    GTTGGAAGGC CTGGTCCTTT GGGGAAAAAG GGAGAACCTG GGATTCCTGG AGATCCAGGG
    CCAGCGGGGC AAGTGGGACA GCGAGGAAAG CAGGGTGACT ATGGCATCCC AGGCTATGGT
    CCTATGGGAG GAAAAGGAGT CAAGGGTCCA CGAGGATTCC CTGGAGATAT GGGCCAAAAG
    GGTGATACTG GTGATCCTGG AATTCCTGGG GGGCCTGGAC CCAAAGGATT TAGGGGATTC
    ACACTCACGG TAGGCCTGAA AGGTGAAGCG GGCTCTCCTG GAAACCAAGG CCCCCCTGGA
    CGGAGAGGCC CTAAAGGAAT GGCAGGGAAG CCCATATATT CTCAATGTGA TCTAATCCAG
    TTCACGCGGG ACCATAGTCC TTGTTGGAAA GAAAAGTGTC CTGTGTATCC AACAGAGCTG
    GTATTTGCCC TGGACCAGTC CAACGATATC ACAGAGCAGA GATTTAATGA AATGAGGGAC
    ATCATCACTT CTATTGTCAA TGACCTTCAC ATCAGGGAAA GTAATTGCCC CGAGGGAGCC
    AGAGTTGTTG TGGTTTCCTA CAACTCGGGC ACCGGCTACC TCATCCATGG GTCTGACTAC
    CGTAGCAAGA AGCAGCTTCT TCAGCTTCTC TCCCAAATAA AATATCAAAG TTCTGGGGAA
    GTCCGAGACA TTGGGAACGC GATGAGGTTT GTGGCTCGGA ACATCTTTAA GCGGACGCCT
    GCAGGAGCCA ATGTGCAGAG AGTTGCTGTG TTTTTTAGCA ACGGACAAGC TGCGAGCAGG
    TCATCTGTCA TCACAGCCAC CATGGAGTTC AGTGCCTTGG ATATCAGTCC TGCAGTCTTC
    GCTTTTAATG AAAAGGTTTA CCTCGATGAG GCTTTTGGGT TTGACAACAC AGGAACATTT
    CAGGTGCTTT CTGTTCCTCC AAATGGGGAA TATGATCCAT TAGAAAGGCT TCGGCGCTGT
    ACACTTTGCT ATGATAAATG TTTTCCAAAT ACTTGCAAGA AAGAAATTGT CATACCTGAA
    AATTCATACA TGGATGTCGC CTTCCTCTTA GACAATTCTA GAAATGTAGC AAGTGATGAG
    TTTAAGGATA TGAAAGCCTT GGTGAGCTCA ATGCTTGACA ACTTTGAAAT TGCCTCAGAC
    CCTATAGTCT CAGACTCTGG TGATAGGATT GCCTTGTTGA GCTATTCTCC TTGGGATAGG
    AGAAAGAAGA ATGTGGTAAA AACAGAGTTT GAGTTTACAA CTTATAACAG TCAAGCTCTG
    ATGAAAAGGT ACATTGAGAC TAACCTCCGA CAGCTAAATG GGGAAGCCAC CATTGGCCAT
    GCCCTACTGT GGGTCGTGGA GAATCTCTTC CCAGCAATGC CGAAGCTAAG AAAATACAGG
    GTCATCTTTG TGGTCTCCGC TGGAGAGAAT CGTGAGAGAA AGGAATTCTT GAAGAAGATG
    GCTCTGAGGG CCAAGTGTCA AGGCTATGTC ATATTTGTGA TTTCCCTGGG TTTGACATCC
    GAGGAGGACA TGGAGGAGCT GGCCAGCCAC CCGCTTGATC ACCATCTGAT ACAGCTGGGG
    AGAATTCATA AACCAAATCT GGATTATGTT GTGAAGTTTT TAAAGCCGTT TGTGTACTCA
    GTGAGACGAG GATTCAATCA GTACCCACCA CCAGTGCTTG AGAACATCTG CCAACTCATT
    GATTCAGAAG ACGAGGAAAA TCAAGGCATC GGTCTCCCAT TTACTCCTGA ACCTTATGAG
    ATTTCTTCAG GAGAGAACAT CATTGGCCAG GAATTGAGTG CTCTGAGAGA TTCATCTTTT
    GTATTGGAGG ACAATGGAAG CGACCATTTG GTTTATGTTC CAAGCCAGAT GCTTATGCCA
    CAGAAATTAA TGACTAAGTA TGAAAAAGAT TGGAATTCTG AAGCAACTGC AAGTCTCACA
    TCTGGACATG AAAACCTTGG CAGAAAAGAA GAACCAGGTC TTACTTATGA AAGTAGAGAT
    GCCTCTCTTC AAGAATATTA CATGGATGTG ACTTTTCTCA TAGATGCTTC CAAAAGAATA
    GGAAGTGATG AGTTTAAGGA AGTGAAAGCT TTTGTAACCT CAGTGCTTGA TTATTTTCAT
    CTGGCCCCAG ATCCACTGAC CTCCACCTTA GGAGACAGAA TAGCAGTCCT GACCTACTCT
    CCTCCAGGCT ATATGCCCAA CACAGAAAAA TGCCCCGTCT ACCTAGAATT TGATTTGGTC
    ACTTATAACA ATATACACCA AATGAAACAT CATCTCCAAG ACTCCCTTCA GCAGCTCAAT
    GGAGATGTTT TTATAGGCCA CGCCCTTCAG TGGACACTTG ACAATGTCTT TGTAGGAACT
    CCCAACCTGA GGAGAAACAA AGTTATCTTT GTTATATCCG CTGGTGAAAC CAACCCTTTA
    GACAGGGAAG TCTTAAGAAA TGTATCTCTG AGAGCCAAGT GTCAGGGCTA CTCCATATTT
    GTGTTTTCTT TTGGTCCTCT ACACAATGAC AAGGAATTAG AAGAATTAGC CAGCCACCCA
    CTGGATCATC ACTTAGTCCA GCTTGGCCGA ACCCACAAGC CAGATTTGAA CTATATCATC
    AAATTTATCA AGCCATTTGT TCATTCAATC AGACGTGGCA TCAACAAATA CCCCCCTGCA
    GACATGAAAC CCAAATGTGT TAACATCACC TCTCCCACTC CAGAGAATAA TGGCATAGGA
    AACACTGTAT TCCTTCTTCC TGAGGTATAT GAAATTGAGA TGGAGAACAG TGAGCTTTCG
    GGTGACCCCG ATTCCCAGCA GCAGCATTTC TTTGAAATAG GGAACAGTCA TAGTAACAGT
    TCTGGTATCA ACACTAATTT GATCCAGAGG TTATACATGC TCTTTTCATC TGGGGAACTG
    ATGATGCAAG ACAAGAAAGA GGCACATTCA GAAGAAATTA CATCTCCAGC AAATGACAGA
    CAAGATGAAA AAGATGGTGA AGACACAAGA TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.