LOC780933 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC780933
Entrez Gene ID	780933
Full Name	cationic trypsin
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001113727.1	NP_001107199.1	cationic trypsin precursor
NM_001113727.2	NP_001107199.1	cationic trypsin precursor

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Prss3	NP_775150.1
Xenopus tropicalis (tropical clawed frog)	LOC100498083	XP_002943534.2
Bos taurus (cattle)	PRSS1	XP_002687054.3
Gallus gallus (chicken)	PRSS3	NP_990716.1
Bos taurus (cattle)	LOC780933	NP_001107199.1
Gallus gallus (chicken)	LOC100857124	XP_003640494.2
Xenopus tropicalis (tropical clawed frog)	prss1	NP_001015792.1
Canis lupus familiaris (dog)	LOC475521	XP_532744.1
Mus musculus (house mouse)	2210010C04Rik	NP_075822.3
Gallus gallus (chicken)	LOC768817	XP_001231433.2
Drosophila melanogaster (fruit fly)	Try29F	NP_523518.2
Xenopus tropicalis (tropical clawed frog)	prss2	NP_001011202.1
Xenopus tropicalis (tropical clawed frog)	LOC101730988	XP_004920306.1
Pan troglodytes (chimpanzee)	LOC100616521	XP_003951209.1
Danio rerio (zebrafish)	try	NP_571783.1
Xenopus tropicalis (tropical clawed frog)	LOC496623	NP_001011199.1
Xenopus tropicalis (tropical clawed frog)	LOC101732348	XP_004919987.1
Xenopus tropicalis (tropical clawed frog)	LOC101730924	XP_004920305.1

Gene Ontology
Bibliography

Related articles in PubMed

Isothermal titration calorimetry determination of individual rate constants of trypsin catalytic activity.
Aguirre C, Condado-Morales I, Olguin LF, Costas M
Analytical biochemistry47918-27(2015 Jun)

Comparison of complexes formed by a crustacean and a vertebrate trypsin with bovine pancreatic trypsin inhibitor - the key to achieving extreme stability?
Moln?r T, V?r?s J, Szeder B, Tak?ts K, Kardos J, Katona G, Gr?f L
The FEBS journal280(22)5750-63(2013 Nov)

Streptomyces erythraeus trypsin inactivates ?1-antitrypsin.
Vukoti KM, Kadiyala CS, Miyagi M
FEBS letters585(24)3898-902(2011 Dec)

X-ray and neutron protein crystallographic analysis of the trypsin-BPTI complex.
Kawamura K, Yamada T, Kurihara K, Tamada T, Kuroki R, Tanaka I, Takahashi H, Niimura N
Acta crystallographica. Section D, Biological crystallography67(Pt 2)140-8(2011 Feb)

Combined use of computational chemistry, NMR screening, and X-ray crystallography for identification and characterization of fluorophilic protein environments.
Vulpetti A, Schiering N, Dalvit C
Proteins78(16)3281-91(2010 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Isothermal titration calorimetry was used to obtain Michaelis-Menten plots for the trypsin-catalyzed hydrolysis of several substrates at different temperatures (278-318K).
Title: Isothermal titration calorimetry determination of individual rate constants of trypsin catalytic activity.

The identification of fluorinated fragments binding to the serine protease trypsin, and the X-ray structures of the complexes are presented.
Title: Combined use of computational chemistry, NMR screening, and X-ray crystallography for identification and characterization of fluorophilic protein environments.

Results describe the functional and structural roles of the Cys14-Cys38 disulfide of bovine pancreatic trypsin inhibitor.
Title: Functional and structural roles of the Cys14-Cys38 disulfide of bovine pancreatic trypsin inhibitor.

The following LOC780933 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC780933 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa44203	NM_001113727.2 Latest version!	Bos taurus cationic trypsin (LOC780933), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
View Old Accession Versions >>
OBa44203	NM_001113727.1	Bos taurus cationic trypsin (LOC780933), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC780933 ( NM_001113727.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001107199.1 Bos taurus cationic trypsin (LOC780933), mRNA.

Price & Availability↑

CloneID	OBa44203
Clone ID Related Accession (Same CDS sequence)	NM_001113727.1 , NM_001113727.2
Accession Version	NM_001113727.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 741bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-26
Organism	Bos taurus(cattle)
Product	cationic trypsin precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC134797.1. On Jan 22, 2008 this sequence version replaced XM_584594.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC134797.1, EV646061.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN02822088, SAMN02822095 [ECO:0000350] ##Evidence-Data-END##

ATGAAGACCT TCATCTTTCT GGCTCTCTTG GGAGCCGCTG TTGCTTTCCC CGTGGACGAT 
GATGACAAGA TCGTGGGCGG CTACACCTGT GGGGCAAATA CTGTCCCCTA CCAAGTGTCC 
CTGAACTCTG GCTACCACTT CTGCGGGGGC TCCCTCATCA ACAGCCAGTG GGTGGTGTCT 
GCGGCTCACT GCTACAAGTC CGGAATCCAA GTGCGTCTGG GAGAAGACAA CATTAATGTC 
GTTGAGGGCA ATGAGCAATT CATCAGCGCA TCCAAGAGTA TCGTCCATCC CAGCTACAAC 
TCAAACACCT TAAACAACGA CATCATGCTG ATTAAACTGA AATCAGCTGC CAGTCTCAAC 
AGCCGAGTAG CCTCTATCTC TCTGCCAACA TCCTGTGCCT CTGCTGGCAC CCAGTGTCTC 
ATCTCTGGCT GGGGCAACAC CAAAAGCAGT GGCACCAGCT ACCCTGATGT CCTGAAGTGT 
CTGAAGGCTC CCATCCTATC AGACAGCTCT TGCAAAAGTG CCTACCCAGG CCAGATCACC 
AGCAACATGT TCTGTGCGGG CTACCTGGAG GGCGGAAAGG ACTCCTGCCA GGGTGACTCC 
GGTGGCCCTG TGGTCTGCAG TGGAAAGCTC CAGGGCATTG TCTCCTGGGG CTCTGGCTGC 
GCTCAGAAAA ACAAGCCTGG TGTCTACACC AAGGTCTGCA ACTACGTGAG CTGGATTAAG 
CAGACCATCG CCTCCAACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001107199.1
CDS	31..771
Translation	MKTFIFLALLGAAVAFPVDDDDKIVGGYTCGANTVPYQVSLNSGYHFCGGSLINSQWVVSAAHCYKSGIQVRLGEDNINVVEGNEQFISASKSIVHPSYNSNTLNNDIMLIKLKSAASLNSRVASISLPTSCASAGTQCLISGWGNTKSSGTSYPDVLKCLKAPILSDSSCKSAYPGQITSNMFCAGYLEGGKDSCQGDSGGPVVCSGKLQGIVSWGSGCAQKNKPGVYTKVCNYVSWIKQTIASN

Target ORF information:

RefSeq Version	NM_001113727.1
Organism	Bos taurus(cattle)
Definition	Bos taurus cationic trypsin (LOC780933), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001113727.1

ORF Insert Sequence:

ATGAAGACCT TCATCTTTCT GGCTCTCTTG GGAGCCGCTG TTGCTTTCCC CGTGGACGAT 
GATGACAAGA TCGTGGGCGG CTACACCTGT GGGGCAAATA CTGTCCCCTA CCAAGTGTCC 
CTGAACTCTG GCTACCACTT CTGCGGGGGC TCCCTCATCA ACAGCCAGTG GGTGGTGTCT 
GCGGCTCACT GCTACAAGTC CGGAATCCAA GTGCGTCTGG GAGAAGACAA CATTAATGTC 
GTTGAGGGCA ATGAGCAATT CATCAGCGCA TCCAAGAGTA TCGTCCATCC CAGCTACAAC 
TCAAACACCT TAAACAACGA CATCATGCTG ATTAAACTGA AATCAGCTGC CAGTCTCAAC 
AGCCGAGTAG CCTCTATCTC TCTGCCAACA TCCTGTGCCT CTGCTGGCAC CCAGTGTCTC 
ATCTCTGGCT GGGGCAACAC CAAAAGCAGT GGCACCAGCT ACCCTGATGT CCTGAAGTGT 
CTGAAGGCTC CCATCCTATC AGACAGCTCT TGCAAAAGTG CCTACCCAGG CCAGATCACC 
AGCAACATGT TCTGTGCGGG CTACCTGGAG GGCGGAAAGG ACTCCTGCCA GGGTGACTCC 
GGTGGCCCTG TGGTCTGCAG TGGAAAGCTC CAGGGCATTG TCTCCTGGGG CTCTGGCTGC 
GCTCAGAAAA ACAAGCCTGG TGTCTACACC AAGGTCTGCA ACTACGTGAG CTGGATTAAG 
CAGACCATCG CCTCCAACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC780933 ( NM_001113727.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001107199.1 Bos taurus cationic trypsin (LOC780933), mRNA.

Price & Availability↑

CloneID	OBa44203
Clone ID Related Accession (Same CDS sequence)	NM_001113727.1 , NM_001113727.2
Accession Version	NM_001113727.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 741bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-19
Organism	Bos taurus(cattle)
Product	cationic trypsin precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC134797.1. On Mar 1, 2018 this sequence version replaced NM_001113727.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC134797.1, EV646061.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145418, SAMN03145419 [ECO:0000350] ##Evidence-Data-END##

ATGAAGACCT TCATCTTTCT GGCTCTCTTG GGAGCCGCTG TTGCTTTCCC CGTGGACGAT 
GATGACAAGA TCGTGGGCGG CTACACCTGT GGGGCAAATA CTGTCCCCTA CCAAGTGTCC 
CTGAACTCTG GCTACCACTT CTGCGGGGGC TCCCTCATCA ACAGCCAGTG GGTGGTGTCT 
GCGGCTCACT GCTACAAGTC CGGAATCCAA GTGCGTCTGG GAGAAGACAA CATTAATGTC 
GTTGAGGGCA ATGAGCAATT CATCAGCGCA TCCAAGAGTA TCGTCCATCC CAGCTACAAC 
TCAAACACCT TAAACAACGA CATCATGCTG ATTAAACTGA AATCAGCTGC CAGTCTCAAC 
AGCCGAGTAG CCTCTATCTC TCTGCCAACA TCCTGTGCCT CTGCTGGCAC CCAGTGTCTC 
ATCTCTGGCT GGGGCAACAC CAAAAGCAGT GGCACCAGCT ACCCTGATGT CCTGAAGTGT 
CTGAAGGCTC CCATCCTATC AGACAGCTCT TGCAAAAGTG CCTACCCAGG CCAGATCACC 
AGCAACATGT TCTGTGCGGG CTACCTGGAG GGCGGAAAGG ACTCCTGCCA GGGTGACTCC 
GGTGGCCCTG TGGTCTGCAG TGGAAAGCTC CAGGGCATTG TCTCCTGGGG CTCTGGCTGC 
GCTCAGAAAA ACAAGCCTGG TGTCTACACC AAGGTCTGCA ACTACGTGAG CTGGATTAAG 
CAGACCATCG CCTCCAACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001107199.1
CDS	30..770
Translation	MKTFIFLALLGAAVAFPVDDDDKIVGGYTCGANTVPYQVSLNSGYHFCGGSLINSQWVVSAAHCYKSGIQVRLGEDNINVVEGNEQFISASKSIVHPSYNSNTLNNDIMLIKLKSAASLNSRVASISLPTSCASAGTQCLISGWGNTKSSGTSYPDVLKCLKAPILSDSSCKSAYPGQITSNMFCAGYLEGGKDSCQGDSGGPVVCSGKLQGIVSWGSGCAQKNKPGVYTKVCNYVSWIKQTIASN

Target ORF information:

RefSeq Version	NM_001113727.2
Organism	Bos taurus(cattle)
Definition	Bos taurus cationic trypsin (LOC780933), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001113727.2

ORF Insert Sequence:

ATGAAGACCT TCATCTTTCT GGCTCTCTTG GGAGCCGCTG TTGCTTTCCC CGTGGACGAT 
GATGACAAGA TCGTGGGCGG CTACACCTGT GGGGCAAATA CTGTCCCCTA CCAAGTGTCC 
CTGAACTCTG GCTACCACTT CTGCGGGGGC TCCCTCATCA ACAGCCAGTG GGTGGTGTCT 
GCGGCTCACT GCTACAAGTC CGGAATCCAA GTGCGTCTGG GAGAAGACAA CATTAATGTC 
GTTGAGGGCA ATGAGCAATT CATCAGCGCA TCCAAGAGTA TCGTCCATCC CAGCTACAAC 
TCAAACACCT TAAACAACGA CATCATGCTG ATTAAACTGA AATCAGCTGC CAGTCTCAAC 
AGCCGAGTAG CCTCTATCTC TCTGCCAACA TCCTGTGCCT CTGCTGGCAC CCAGTGTCTC 
ATCTCTGGCT GGGGCAACAC CAAAAGCAGT GGCACCAGCT ACCCTGATGT CCTGAAGTGT 
CTGAAGGCTC CCATCCTATC AGACAGCTCT TGCAAAAGTG CCTACCCAGG CCAGATCACC 
AGCAACATGT TCTGTGCGGG CTACCTGGAG GGCGGAAAGG ACTCCTGCCA GGGTGACTCC 
GGTGGCCCTG TGGTCTGCAG TGGAAAGCTC CAGGGCATTG TCTCCTGGGG CTCTGGCTGC 
GCTCAGAAAA ACAAGCCTGG TGTCTACACC AAGGTCTGCA ACTACGTGAG CTGGATTAAG 
CAGACCATCG CCTCCAACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Isothermal titration calorimetry determination of individual rate constants of trypsin catalytic activity.
Analytical biochemistry47918-27(2015 Jun)
Aguirre C,Condado-Morales I,Olguin LF,Costas M

Comparison of complexes formed by a crustacean and a vertebrate trypsin with bovine pancreatic trypsin inhibitor - the key to achieving extreme stability?
The FEBS journal280(22)5750-63(2013 Nov)
Moln?r T,V?r?s J,Szeder B,Tak?ts K,Kardos J,Katona G,Gr?f L

Streptomyces erythraeus trypsin inactivates ?1-antitrypsin.
FEBS letters585(24)3898-902(2011 Dec)
Vukoti KM,Kadiyala CS,Miyagi M

X-ray and neutron protein crystallographic analysis of the trypsin-BPTI complex.
Acta crystallographica. Section D, Biological crystallography67(Pt 2)140-8(2011 Feb)
Kawamura K,Yamada T,Kurihara K,Tamada T,Kuroki R,Tanaka I,Takahashi H,Niimura N

Combined use of computational chemistry, NMR screening, and X-ray crystallography for identification and characterization of fluorophilic protein environments.
Proteins78(16)3281-91(2010 Dec)
Vulpetti A,Schiering N,Dalvit C

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LOC780933 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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