CFAP74 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	CFAP74
Entrez Gene ID	617386
Full Name	cilia and flagella associated protein 74
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_024976795.1	XP_024832563.1	cilia- and flagella-associated protein 74 isoform X5
XM_024976791.1	XP_024832559.1	cilia- and flagella-associated protein 74 isoform X1
XM_015466809.1	XP_015322295.1	cilia- and flagella-associated protein 74
XM_015475230.1	XP_015330716.1	cilia- and flagella-associated protein 74
XM_024976790.1	XP_024832558.1	cilia- and flagella-associated protein 74 isoform X1
XM_024976792.1	XP_024832560.1	cilia- and flagella-associated protein 74 isoform X2
XM_024976793.1	XP_024832561.1	cilia- and flagella-associated protein 74 isoform X3
XM_024976794.1	XP_024832562.1	cilia- and flagella-associated protein 74 isoform X4

Pathways from BioSystems
Gene Ontology

The following CFAP74 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CFAP74 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa56327	XM_024976790.1 Latest version!	Bos taurus cilia and flagella associated protein 74 (CFAP74), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBa56327	XM_024976791.1 Latest version!	Bos taurus cilia and flagella associated protein 74 (CFAP74), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBa56328	XM_024976792.1 Latest version!	Bos taurus cilia and flagella associated protein 74 (CFAP74), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBa56330	XM_024976793.1 Latest version!	Bos taurus cilia and flagella associated protein 74 (CFAP74), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OBa56331	XM_024976794.1 Latest version!	Bos taurus cilia and flagella associated protein 74 (CFAP74), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OBa56332	XM_024976795.1 Latest version!	Bos taurus cilia and flagella associated protein 74 (CFAP74), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OBa93026	XM_015466809.1 Latest version!	Bos taurus cilia and flagella associated protein 74 (CFAP74), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBa93026	XM_015475230.1 Latest version!	Bos taurus cilia and flagella associated protein 74 (CFAP74), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CFAP74 ( XM_024976791.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024832559.1 Bos taurus cilia and flagella associated protein 74 (CFAP74), transcript variant X2, mRNA.

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CloneID	OBa56327
Clone ID Related Accession (Same CDS sequence)	XM_024976791.1 , XM_024976790.1
Accession Version	XM_024976791.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5172bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	cilia- and flagella-associated protein 74 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAAG ACGACAAGAA GCTTTTGGAT GACAAGCCGC TCGTGGAAGA TGACACAGAG 
GAGCTGGAGG ACCCAGAGCT TGTGCTGGAC TGTGTCCCCA GGGAGGCTGA GCAGAAGGCC 
GGCCTGGGAA GCTCCTGTCC AGGAATAGAG CAGAGCAAGG ACAAGCAGAA GCAGAGGTTG 
ACTGCTCAGG AGAAAAGGCA GGCCTGCCTG CTGCGCAGGG ACTTGAGCCA CCTGGACCAG 
CTGCATGAAG AGAAAGACCT TTTCATCCAG AAGACAAGGG GGGAGCTACG TGCCTGCCGC 
AGGAGGATGG AACTCCTAGT CAAGCAGCAG GAGAGTGTGG CAGCCGAGAT AGCTGCAGAG 
AGGGCAGCCA ACAACATGGC AGCCATGGGC CGCCTGGAGG CAGCGTCGAG GCGCCTGTGC 
TCAGAGCTGA AGAACGAGCA GGACCTGCAG GCGCAGATGA TGGCGGTGCT GCAGCAGAGC 
CAGAGCGCCC TGTGGCAGAT TGAGGTCCAG GAGGGGCAGC TAGAGGACGC CCGCAAGTCG 
GCCCAAGAGG AGGCTGCAGC CGCCAGGAGA GGCCTTCAGG AGCGGGCAGC CAAGCAGCTG 
CACCGGGAGA TGGGGGCCTC GGGGAAGGCA GAGCGGAACA GGCTGCTGCG AGCCCGGAGA 
TGCGTGCACC TGCAGAAGGA GCTTGGACTC CGGCAGCAGA AGCTGGTGGA GGAAGCCCAG 
AAGAACCACC GGAAGGCCCT GAGGTTCCTG AAGGCCTCGT TGGGGAGGAT CCGTGAGCAG 
GAGCAGAAGG AGGAGCTGGA GACCCGAGAG CACATGCGCA GGCGCATGGA CGCGGTGCTG 
GCCCTGAAGA ACAGTATCTC CGCCAACCGG GAAACGCTCC GGAAATTTCA AGCATGGGGC 
CAGGCCAAGA CAGAGCTGGC AGAACAGAAG GCCCAGGCGG AGAAGAAGGC AATTCTGGCC 
CAAGGTGGGG ACGCCTTTAA GCACCTGTGC CATCAGCGCC GGCGCCAGGA GTTGGAGGCC 
CAGAACCGCG CCTTCGAGGA GGAGCAGAAG CGCAGGAAGC AGGAGATTGT GAGTCGGCTC 
TTGAAGGAAG AGGCTGTGGA GGACCGGAGG AGGCCACGGC CCGAGCCCCC GAGACCCGCA 
GGCCAGCGGA GCCTGAGGGA CCAGACCTGG AGCTACGTGG CCACCTTCTT CGATGGGCAG 
AGCACAGAGG CCCCGCTCTG CTGTCCCCTG CCTGATGTCC CTGGACAGAG GCCAGGTCCA 
GCCCAAGAGC AGATAAAGCT CCAGGAGACC AGGTCCAGAT TCTACCAGTC CTCTCCGCTG 
CTGACGGCCA TCTCCAGTGA GTCTGTCCAG GGGGACGGGA GCCTTGGCGG GGAGGACACA 
CTGGCTTGGC CGGAGATCCC CGGGCTCTGG AAGGAGGACT ACAAGCCTTT CCAGGTATCC 
AAGGAGGATG TGGACCGGAA GCCAGTGGGC GGAACAAAGA TGGACAAGGA CATCCTGGCG 
CGGACAATGC AACGGCTACG CAGTGGGGTG ATCCACAAAC AGGTGGCATC AGGGCGTGAG 
TTCAGAGGGT GCCCCTTCAA CAGCAAGCCC AAGTTCATCC ACTTCAAGGA CTTTGATGTG 
GGCAAAGTGT ACAAGAAGAA GATCACGCTG ATCAACGCCA CCTACACGAT TAACTACTGC 
AAGCTGGTGG GCGTGGATGA GAGCCTGCAG GACTTCATCC ACGTGGACTT TGACCCCCCA 
GGCCCACTAT CAGCCGGCAT GTCCTGCGAA GTGCTTGTCA CCTTCAAGCC CATGATAAAC 
AAAGATCTGG AAGGAAATGT CTCGTTTCTG GCTCAGACAG GCGGATTTTC TGTTCCACTG 
AAATGTTCAA CAAAGAAATG CTCTCTGTCC CTCGACAAGG AGCTCATCGA CTTTGGCAGC 
TACACAGTGG GTGAGACTGC AATCCGGACC ATCACGCTGA CCAACACTGG GGGCTTGGGC 
ACCAAGTTCA GATTTCTTCT GGCATCAGAG ACTTGTGAGA TGGACACCTC CCAGTCGGTC 
CTAAAATTAA GTAGTACCTT CACGTATGAA GACAAAAGTT TGTATGATAA ATTCACCACC 
AGTTTTTCTG AGCATCAGAT GGAGGGCAAT GAGTCATCTC CTACAGATGT ACCAAGCCAG 
AAGGAGTCTG GAAAGCTGGA CGTGAAGGAG CAGGAGGAGG GGCACCCCAT CGAATCAGAG 
GGCGTGACCT TGACCACCAT CATGACTATA CCTCTCAATG AGGAACAGAT GGAGTTCTCG 
CTGGGAGAGA CAACAGAGGG GGACATAGGC CCCTTCAGCT CCATCAAGGT GCCCGTCATC 
TTCACCCCGA TTGTCCCGGG AACGGTCCAG AACAGTTTCA AGGTTGTGTT TAAGAATCAG 
CAGTGCCCAA CTTTATATTT CAAAGTCGTC GGCGTGGCTA TTGATGTGCC TGTCTGGGTG 
CCAAAGCCCA ACGTAGACCT GAAGATCTGC ATGTACGATC GGCTGTACCA GGACTCGATC 
CTCGTCCACA CGCGGTCGAA GGCCGCGTTG CGCCTAAAGT TCGAGGTGTG CAAGGAGCTG 
CGGGGCCACG TGGAGCTCCT GCCTGAGACT GGCTACATCC AGGCCCTGTC GTCCTACTCA 
GTGCAGCTCA AGTTCCTGCC CAGACACTCC CTCCCAGAAG ATGCAGGGAA GTATTTTGAC 
AAGGAGACCA GGGTTCTGGA GGCCCCGATG ACTATCCGGG TGGCTGACCA GATCAAACCC 
GTGGGGTTTA CTGTCCATGC TGTAGTCACC ACCTCAGACC TGGAGCTCAG CCCCGCAGGG 
CTGGACTTTG GCTACTGTAC CATCTACGAG GCCATCAGGA CCCAAATCAG CCTCTGCAAT 
CATTCCCTCC TGCCCCAGGA GTTTGGGTTC GTTGGACTCC CCAAGTTTGT GGACATCCAG 
CCCAACGATG GGTTCGGGAC AATCCTGCCA CTGGAAACGC TACAGCTCGA TGTGATCTTT 
CAGCCCACCA AGGCCAAGGA GTACAACTTT GAGCTCATCT GCAAGTCAGA GATTAATCGG 
TGCTTCAAGC TGGCATGCCG GGCCGTGGGT GTCTACCCAC CCCTGGAGCT GTCCCACAAC 
CAGATCAAGT TTGCAGCCAC CTCCTTGTAC GACACATCCG TGGCCACTCT GTACGTCATC 
AACTCCCACC TGAGCATGAA CTCACTGACC CACTCAGTGC CCCGCATTGG CTCAGAGGAC 
GCTTTTCCCG TGGGGCCCAC GTCTTTTGAG TTCCTGCTGC CCCCAGATTC GCCCATCACC 
ATCTCACCCC TGGTGGGCAC CGTGTGGCCA GGAAAGAGGT GCCTGGTCCG AGTGGCCTTC 
CGGCCAGTGC TGCCCAGCGA GCTGATCCAC CAGGAGGCCT TCCAGGCCCT GAGCAGAAAA 
GCTGAGGTCA AATCGTCCCA AAAGGAGACG GTCACCCAGA GGAAGGACCA GCGGAGACCA 
TCTTTCTCCA TGCTGCGGCT GCAGAACCAA GACCGGCTGC TCCGGGCCTC AGCCTCCCAG 
CCCCCGGAGC TGCAGAAGGA GGACCTTGCA TCCAGCTCTC AAGAGTACCA GTCGGCACAG 
GCCTCCCTGA CCAGGTCTTT CCAGGGGAAG TTTGACAAGT TTGTGGTTCC CTGTGTTGTT 
GCTAGCGGCG ACACCAAAGA CAGGAAGGGG ACAGAACCGC TGAGCTTCAG TCCCCACAAC 
ACGCTGTACC TGGAGCTGTG GTGTCCAGCA GTGGCGCCGT CTATCGTGGT GACGTCCGAC 
AAAGGCAAGA CCATCATTAA CTTCGGCGAC ATTGCTGCAG GACACCGGGG CATAAAGAAG 
GTCTCCATCC AGAACATCTC CCCTGAGGAT CTCGACCTGG AGTACTCCGT GCTGAACCCC 
GATGGCCCCT TCGTCCTGCT GAACCCCACC ATCAGGTTGC CCTCTGGCGA GACCCAGGTC 
CTAGATCTGT CCTTCTCCCC ACGTGAGAGT GTCGTGGCCC AAGAAATACT GGACATCATC 
ACCAAGAAAG GCACGCTCTC CCTGGCCCTC ATGGGCACGG GTGTGGCGTC CACGATCACG 
TGCTCCATTG AAGGTGATGT CCTCAACATG GGCTACGTGA TCGCCAGAGA GTCTGTCTCC 
TTGGGCTTCA AGCTGCAGAA CAACTCCCCG CTGCCCATCA AGTTCTCCAT GCAGCTGGAC 
AGCCTCTCCT CCAGGAGCAG AGACCAGCAC CGCCTGCCAC AGTTCCTGGC CTCGCCTGAC 
CAGAGGACGG AAGTCGTGGG CACGCAGAAC TACAGTGGCC AGAGCGTGTT CAGCGTGGTC 
CCGGTCGAGG GCGTCATGGA GCCCGGCAAG GCTCAGGACT TCACTGTGAC CTTCAGCCCA 
GACCACGAGA GCCTATACTT CTCTGACCGG CTGCAGGTCG TGCTCTTTGA AAAGGCAGGA 
CTCCGACCCA GCCTGGACCC CTCCTCCCCA CCCGCATGTC CATTTGTCCA AGCCCTTTGG 
CTGCCTGAGC GGCCCCACAT TGGAGCTTGG TGCCTGGGGG GGCTCCACAT CAGGCTGGGG 
GTCATCAGGT GGAGAGACCA ATGTCTGGGG TCCCACAGGC CCACATCACA GCCCCGCCCC 
CTCCCAGAGC CCCACATGCA GGGTTGCAAA CCGCTGTGCT CCTGCCCCCA GAAAGTGTCC 
CACCACATCC TCCTGAAGGG CGCGGCCCGC GAGCACATGA TGTTCGTGGA GGGCGGAGAC 
CCCCTGGACG TGCCCGTGGA GTCCCTGACC GTGATCCCTG CCTTTGACCT GGAGCACAGA 
GAGGAAGCTG AGGAGCTAAA GCCCATCCTG GTGACCCTGG ACTACGCCCA GCTGGACCCA 
GACACACAGG CCCCACCTGC CACCCGAGAA CTGCAGGTGG GCTGCATCCG GACTACCCAG 
CTGTCCGCAA AGAAGACTGT TGAGTTCAGC ATGGACAACA TCGCAGCCCT GCAGCACAAG 
GGCTTCACCA TTGAGCCCTC CAGAGGCTCT GTGGAGCGCG GCCAGACCAA GGCCATCAGC 
ATCTCCTGGA CACCACCCAT CGACTTTGAT CCGGACCACC CACTCTTGGC ATCGGCCCTG 
CTGCAGCTCA GGGGGGATGT GAAGGAGACC TACAAGGTCC TCTTTGTAGC CCAGGTGGTG 
ACCGGTCCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024832559.1
CDS	171..5342
Translation	MEEDDKKLLDDKPLVEDDTEELEDPELVLDCVPREAEQKAGLGSSCPGIEQSKDKQKQRLTAQEKRQACLLRRDLSHLDQLHEEKDLFIQKTRGELRACRRRMELLVKQQESVAAEIAAERAANNMAAMGRLEAASRRLCSELKNEQDLQAQMMAVLQQSQSALWQIEVQEGQLEDARKSAQEEAAAARRGLQERAAKQLHREMGASGKAERNRLLRARRCVHLQKELGLRQQKLVEEAQKNHRKALRFLKASLGRIREQEQKEELETREHMRRRMDAVLALKNSISANRETLRKFQAWGQAKTELAEQKAQAEKKAILAQGGDAFKHLCHQRRRQELEAQNRAFEEEQKRRKQEIVSRLLKEEAVEDRRRPRPEPPRPAGQRSLRDQTWSYVATFFDGQSTEAPLCCPLPDVPGQRPGPAQEQIKLQETRSRFYQSSPLLTAISSESVQGDGSLGGEDTLAWPEIPGLWKEDYKPFQVSKEDVDRKPVGGTKMDKDILARTMQRLRSGVIHKQVASGREFRGCPFNSKPKFIHFKDFDVGKVYKKKITLINATYTINYCKLVGVDESLQDFIHVDFDPPGPLSAGMSCEVLVTFKPMINKDLEGNVSFLAQTGGFSVPLKCSTKKCSLSLDKELIDFGSYTVGETAIRTITLTNTGGLGTKFRFLLASETCEMDTSQSVLKLSSTFTYEDKSLYDKFTTSFSEHQMEGNESSPTDVPSQKESGKLDVKEQEEGHPIESEGVTLTTIMTIPLNEEQMEFSLGETTEGDIGPFSSIKVPVIFTPIVPGTVQNSFKVVFKNQQCPTLYFKVVGVAIDVPVWVPKPNVDLKICMYDRLYQDSILVHTRSKAALRLKFEVCKELRGHVELLPETGYIQALSSYSVQLKFLPRHSLPEDAGKYFDKETRVLEAPMTIRVADQIKPVGFTVHAVVTTSDLELSPAGLDFGYCTIYEAIRTQISLCNHSLLPQEFGFVGLPKFVDIQPNDGFGTILPLETLQLDVIFQPTKAKEYNFELICKSEINRCFKLACRAVGVYPPLELSHNQIKFAATSLYDTSVATLYVINSHLSMNSLTHSVPRIGSEDAFPVGPTSFEFLLPPDSPITISPLVGTVWPGKRCLVRVAFRPVLPSELIHQEAFQALSRKAEVKSSQKETVTQRKDQRRPSFSMLRLQNQDRLLRASASQPPELQKEDLASSSQEYQSAQASLTRSFQGKFDKFVVPCVVASGDTKDRKGTEPLSFSPHNTLYLELWCPAVAPSIVVTSDKGKTIINFGDIAAGHRGIKKVSIQNISPEDLDLEYSVLNPDGPFVLLNPTIRLPSGETQVLDLSFSPRESVVAQEILDIITKKGTLSLALMGTGVASTITCSIEGDVLNMGYVIARESVSLGFKLQNNSPLPIKFSMQLDSLSSRSRDQHRLPQFLASPDQRTEVVGTQNYSGQSVFSVVPVEGVMEPGKAQDFTVTFSPDHESLYFSDRLQVVLFEKAGLRPSLDPSSPPACPFVQALWLPERPHIGAWCLGGLHIRLGVIRWRDQCLGSHRPTSQPRPLPEPHMQGCKPLCSCPQKVSHHILLKGAAREHMMFVEGGDPLDVPVESLTVIPAFDLEHREEAEELKPILVTLDYAQLDPDTQAPPATRELQVGCIRTTQLSAKKTVEFSMDNIAALQHKGFTIEPSRGSVERGQTKAISISWTPPIDFDPDHPLLASALLQLRGDVKETYKVLFVAQVVTGP

Target ORF information:

RefSeq Version	XM_024976791.1
Organism	Bos taurus(cattle)
Definition	Bos taurus cilia and flagella associated protein 74 (CFAP74), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024976791.1

ORF Insert Sequence:

ATGGAAGAAG ACGACAAGAA GCTTTTGGAT GACAAGCCGC TCGTGGAAGA TGACACAGAG 
GAGCTGGAGG ACCCAGAGCT TGTGCTGGAC TGTGTCCCCA GGGAGGCTGA GCAGAAGGCC 
GGCCTGGGAA GCTCCTGTCC AGGAATAGAG CAGAGCAAGG ACAAGCAGAA GCAGAGGTTG 
ACTGCTCAGG AGAAAAGGCA GGCCTGCCTG CTGCGCAGGG ACTTGAGCCA CCTGGACCAG 
CTGCATGAAG AGAAAGACCT TTTCATCCAG AAGACAAGGG GGGAGCTACG TGCCTGCCGC 
AGGAGGATGG AACTCCTAGT CAAGCAGCAG GAGAGTGTGG CAGCCGAGAT AGCTGCAGAG 
AGGGCAGCCA ACAACATGGC AGCCATGGGC CGCCTGGAGG CAGCGTCGAG GCGCCTGTGC 
TCAGAGCTGA AGAACGAGCA GGACCTGCAG GCGCAGATGA TGGCGGTGCT GCAGCAGAGC 
CAGAGCGCCC TGTGGCAGAT TGAGGTCCAG GAGGGGCAGC TAGAGGACGC CCGCAAGTCG 
GCCCAAGAGG AGGCTGCAGC CGCCAGGAGA GGCCTTCAGG AGCGGGCAGC CAAGCAGCTG 
CACCGGGAGA TGGGGGCCTC GGGGAAGGCA GAGCGGAACA GGCTGCTGCG AGCCCGGAGA 
TGCGTGCACC TGCAGAAGGA GCTTGGACTC CGGCAGCAGA AGCTGGTGGA GGAAGCCCAG 
AAGAACCACC GGAAGGCCCT GAGGTTCCTG AAGGCCTCGT TGGGGAGGAT CCGTGAGCAG 
GAGCAGAAGG AGGAGCTGGA GACCCGAGAG CACATGCGCA GGCGCATGGA CGCGGTGCTG 
GCCCTGAAGA ACAGTATCTC CGCCAACCGG GAAACGCTCC GGAAATTTCA AGCATGGGGC 
CAGGCCAAGA CAGAGCTGGC AGAACAGAAG GCCCAGGCGG AGAAGAAGGC AATTCTGGCC 
CAAGGTGGGG ACGCCTTTAA GCACCTGTGC CATCAGCGCC GGCGCCAGGA GTTGGAGGCC 
CAGAACCGCG CCTTCGAGGA GGAGCAGAAG CGCAGGAAGC AGGAGATTGT GAGTCGGCTC 
TTGAAGGAAG AGGCTGTGGA GGACCGGAGG AGGCCACGGC CCGAGCCCCC GAGACCCGCA 
GGCCAGCGGA GCCTGAGGGA CCAGACCTGG AGCTACGTGG CCACCTTCTT CGATGGGCAG 
AGCACAGAGG CCCCGCTCTG CTGTCCCCTG CCTGATGTCC CTGGACAGAG GCCAGGTCCA 
GCCCAAGAGC AGATAAAGCT CCAGGAGACC AGGTCCAGAT TCTACCAGTC CTCTCCGCTG 
CTGACGGCCA TCTCCAGTGA GTCTGTCCAG GGGGACGGGA GCCTTGGCGG GGAGGACACA 
CTGGCTTGGC CGGAGATCCC CGGGCTCTGG AAGGAGGACT ACAAGCCTTT CCAGGTATCC 
AAGGAGGATG TGGACCGGAA GCCAGTGGGC GGAACAAAGA TGGACAAGGA CATCCTGGCG 
CGGACAATGC AACGGCTACG CAGTGGGGTG ATCCACAAAC AGGTGGCATC AGGGCGTGAG 
TTCAGAGGGT GCCCCTTCAA CAGCAAGCCC AAGTTCATCC ACTTCAAGGA CTTTGATGTG 
GGCAAAGTGT ACAAGAAGAA GATCACGCTG ATCAACGCCA CCTACACGAT TAACTACTGC 
AAGCTGGTGG GCGTGGATGA GAGCCTGCAG GACTTCATCC ACGTGGACTT TGACCCCCCA 
GGCCCACTAT CAGCCGGCAT GTCCTGCGAA GTGCTTGTCA CCTTCAAGCC CATGATAAAC 
AAAGATCTGG AAGGAAATGT CTCGTTTCTG GCTCAGACAG GCGGATTTTC TGTTCCACTG 
AAATGTTCAA CAAAGAAATG CTCTCTGTCC CTCGACAAGG AGCTCATCGA CTTTGGCAGC 
TACACAGTGG GTGAGACTGC AATCCGGACC ATCACGCTGA CCAACACTGG GGGCTTGGGC 
ACCAAGTTCA GATTTCTTCT GGCATCAGAG ACTTGTGAGA TGGACACCTC CCAGTCGGTC 
CTAAAATTAA GTAGTACCTT CACGTATGAA GACAAAAGTT TGTATGATAA ATTCACCACC 
AGTTTTTCTG AGCATCAGAT GGAGGGCAAT GAGTCATCTC CTACAGATGT ACCAAGCCAG 
AAGGAGTCTG GAAAGCTGGA CGTGAAGGAG CAGGAGGAGG GGCACCCCAT CGAATCAGAG 
GGCGTGACCT TGACCACCAT CATGACTATA CCTCTCAATG AGGAACAGAT GGAGTTCTCG 
CTGGGAGAGA CAACAGAGGG GGACATAGGC CCCTTCAGCT CCATCAAGGT GCCCGTCATC 
TTCACCCCGA TTGTCCCGGG AACGGTCCAG AACAGTTTCA AGGTTGTGTT TAAGAATCAG 
CAGTGCCCAA CTTTATATTT CAAAGTCGTC GGCGTGGCTA TTGATGTGCC TGTCTGGGTG 
CCAAAGCCCA ACGTAGACCT GAAGATCTGC ATGTACGATC GGCTGTACCA GGACTCGATC 
CTCGTCCACA CGCGGTCGAA GGCCGCGTTG CGCCTAAAGT TCGAGGTGTG CAAGGAGCTG 
CGGGGCCACG TGGAGCTCCT GCCTGAGACT GGCTACATCC AGGCCCTGTC GTCCTACTCA 
GTGCAGCTCA AGTTCCTGCC CAGACACTCC CTCCCAGAAG ATGCAGGGAA GTATTTTGAC 
AAGGAGACCA GGGTTCTGGA GGCCCCGATG ACTATCCGGG TGGCTGACCA GATCAAACCC 
GTGGGGTTTA CTGTCCATGC TGTAGTCACC ACCTCAGACC TGGAGCTCAG CCCCGCAGGG 
CTGGACTTTG GCTACTGTAC CATCTACGAG GCCATCAGGA CCCAAATCAG CCTCTGCAAT 
CATTCCCTCC TGCCCCAGGA GTTTGGGTTC GTTGGACTCC CCAAGTTTGT GGACATCCAG 
CCCAACGATG GGTTCGGGAC AATCCTGCCA CTGGAAACGC TACAGCTCGA TGTGATCTTT 
CAGCCCACCA AGGCCAAGGA GTACAACTTT GAGCTCATCT GCAAGTCAGA GATTAATCGG 
TGCTTCAAGC TGGCATGCCG GGCCGTGGGT GTCTACCCAC CCCTGGAGCT GTCCCACAAC 
CAGATCAAGT TTGCAGCCAC CTCCTTGTAC GACACATCCG TGGCCACTCT GTACGTCATC 
AACTCCCACC TGAGCATGAA CTCACTGACC CACTCAGTGC CCCGCATTGG CTCAGAGGAC 
GCTTTTCCCG TGGGGCCCAC GTCTTTTGAG TTCCTGCTGC CCCCAGATTC GCCCATCACC 
ATCTCACCCC TGGTGGGCAC CGTGTGGCCA GGAAAGAGGT GCCTGGTCCG AGTGGCCTTC 
CGGCCAGTGC TGCCCAGCGA GCTGATCCAC CAGGAGGCCT TCCAGGCCCT GAGCAGAAAA 
GCTGAGGTCA AATCGTCCCA AAAGGAGACG GTCACCCAGA GGAAGGACCA GCGGAGACCA 
TCTTTCTCCA TGCTGCGGCT GCAGAACCAA GACCGGCTGC TCCGGGCCTC AGCCTCCCAG 
CCCCCGGAGC TGCAGAAGGA GGACCTTGCA TCCAGCTCTC AAGAGTACCA GTCGGCACAG 
GCCTCCCTGA CCAGGTCTTT CCAGGGGAAG TTTGACAAGT TTGTGGTTCC CTGTGTTGTT 
GCTAGCGGCG ACACCAAAGA CAGGAAGGGG ACAGAACCGC TGAGCTTCAG TCCCCACAAC 
ACGCTGTACC TGGAGCTGTG GTGTCCAGCA GTGGCGCCGT CTATCGTGGT GACGTCCGAC 
AAAGGCAAGA CCATCATTAA CTTCGGCGAC ATTGCTGCAG GACACCGGGG CATAAAGAAG 
GTCTCCATCC AGAACATCTC CCCTGAGGAT CTCGACCTGG AGTACTCCGT GCTGAACCCC 
GATGGCCCCT TCGTCCTGCT GAACCCCACC ATCAGGTTGC CCTCTGGCGA GACCCAGGTC 
CTAGATCTGT CCTTCTCCCC ACGTGAGAGT GTCGTGGCCC AAGAAATACT GGACATCATC 
ACCAAGAAAG GCACGCTCTC CCTGGCCCTC ATGGGCACGG GTGTGGCGTC CACGATCACG 
TGCTCCATTG AAGGTGATGT CCTCAACATG GGCTACGTGA TCGCCAGAGA GTCTGTCTCC 
TTGGGCTTCA AGCTGCAGAA CAACTCCCCG CTGCCCATCA AGTTCTCCAT GCAGCTGGAC 
AGCCTCTCCT CCAGGAGCAG AGACCAGCAC CGCCTGCCAC AGTTCCTGGC CTCGCCTGAC 
CAGAGGACGG AAGTCGTGGG CACGCAGAAC TACAGTGGCC AGAGCGTGTT CAGCGTGGTC 
CCGGTCGAGG GCGTCATGGA GCCCGGCAAG GCTCAGGACT TCACTGTGAC CTTCAGCCCA 
GACCACGAGA GCCTATACTT CTCTGACCGG CTGCAGGTCG TGCTCTTTGA AAAGGCAGGA 
CTCCGACCCA GCCTGGACCC CTCCTCCCCA CCCGCATGTC CATTTGTCCA AGCCCTTTGG 
CTGCCTGAGC GGCCCCACAT TGGAGCTTGG TGCCTGGGGG GGCTCCACAT CAGGCTGGGG 
GTCATCAGGT GGAGAGACCA ATGTCTGGGG TCCCACAGGC CCACATCACA GCCCCGCCCC 
CTCCCAGAGC CCCACATGCA GGGTTGCAAA CCGCTGTGCT CCTGCCCCCA GAAAGTGTCC 
CACCACATCC TCCTGAAGGG CGCGGCCCGC GAGCACATGA TGTTCGTGGA GGGCGGAGAC 
CCCCTGGACG TGCCCGTGGA GTCCCTGACC GTGATCCCTG CCTTTGACCT GGAGCACAGA 
GAGGAAGCTG AGGAGCTAAA GCCCATCCTG GTGACCCTGG ACTACGCCCA GCTGGACCCA 
GACACACAGG CCCCACCTGC CACCCGAGAA CTGCAGGTGG GCTGCATCCG GACTACCCAG 
CTGTCCGCAA AGAAGACTGT TGAGTTCAGC ATGGACAACA TCGCAGCCCT GCAGCACAAG 
GGCTTCACCA TTGAGCCCTC CAGAGGCTCT GTGGAGCGCG GCCAGACCAA GGCCATCAGC 
ATCTCCTGGA CACCACCCAT CGACTTTGAT CCGGACCACC CACTCTTGGC ATCGGCCCTG 
CTGCAGCTCA GGGGGGATGT GAAGGAGACC TACAAGGTCC TCTTTGTAGC CCAGGTGGTG 
ACCGGTCCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CFAP74 ( XM_024976790.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024832558.1 Bos taurus cilia and flagella associated protein 74 (CFAP74), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa56327
Clone ID Related Accession (Same CDS sequence)	XM_024976791.1 , XM_024976790.1
Accession Version	XM_024976790.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5172bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	cilia- and flagella-associated protein 74 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAAG ACGACAAGAA GCTTTTGGAT GACAAGCCGC TCGTGGAAGA TGACACAGAG 
GAGCTGGAGG ACCCAGAGCT TGTGCTGGAC TGTGTCCCCA GGGAGGCTGA GCAGAAGGCC 
GGCCTGGGAA GCTCCTGTCC AGGAATAGAG CAGAGCAAGG ACAAGCAGAA GCAGAGGTTG 
ACTGCTCAGG AGAAAAGGCA GGCCTGCCTG CTGCGCAGGG ACTTGAGCCA CCTGGACCAG 
CTGCATGAAG AGAAAGACCT TTTCATCCAG AAGACAAGGG GGGAGCTACG TGCCTGCCGC 
AGGAGGATGG AACTCCTAGT CAAGCAGCAG GAGAGTGTGG CAGCCGAGAT AGCTGCAGAG 
AGGGCAGCCA ACAACATGGC AGCCATGGGC CGCCTGGAGG CAGCGTCGAG GCGCCTGTGC 
TCAGAGCTGA AGAACGAGCA GGACCTGCAG GCGCAGATGA TGGCGGTGCT GCAGCAGAGC 
CAGAGCGCCC TGTGGCAGAT TGAGGTCCAG GAGGGGCAGC TAGAGGACGC CCGCAAGTCG 
GCCCAAGAGG AGGCTGCAGC CGCCAGGAGA GGCCTTCAGG AGCGGGCAGC CAAGCAGCTG 
CACCGGGAGA TGGGGGCCTC GGGGAAGGCA GAGCGGAACA GGCTGCTGCG AGCCCGGAGA 
TGCGTGCACC TGCAGAAGGA GCTTGGACTC CGGCAGCAGA AGCTGGTGGA GGAAGCCCAG 
AAGAACCACC GGAAGGCCCT GAGGTTCCTG AAGGCCTCGT TGGGGAGGAT CCGTGAGCAG 
GAGCAGAAGG AGGAGCTGGA GACCCGAGAG CACATGCGCA GGCGCATGGA CGCGGTGCTG 
GCCCTGAAGA ACAGTATCTC CGCCAACCGG GAAACGCTCC GGAAATTTCA AGCATGGGGC 
CAGGCCAAGA CAGAGCTGGC AGAACAGAAG GCCCAGGCGG AGAAGAAGGC AATTCTGGCC 
CAAGGTGGGG ACGCCTTTAA GCACCTGTGC CATCAGCGCC GGCGCCAGGA GTTGGAGGCC 
CAGAACCGCG CCTTCGAGGA GGAGCAGAAG CGCAGGAAGC AGGAGATTGT GAGTCGGCTC 
TTGAAGGAAG AGGCTGTGGA GGACCGGAGG AGGCCACGGC CCGAGCCCCC GAGACCCGCA 
GGCCAGCGGA GCCTGAGGGA CCAGACCTGG AGCTACGTGG CCACCTTCTT CGATGGGCAG 
AGCACAGAGG CCCCGCTCTG CTGTCCCCTG CCTGATGTCC CTGGACAGAG GCCAGGTCCA 
GCCCAAGAGC AGATAAAGCT CCAGGAGACC AGGTCCAGAT TCTACCAGTC CTCTCCGCTG 
CTGACGGCCA TCTCCAGTGA GTCTGTCCAG GGGGACGGGA GCCTTGGCGG GGAGGACACA 
CTGGCTTGGC CGGAGATCCC CGGGCTCTGG AAGGAGGACT ACAAGCCTTT CCAGGTATCC 
AAGGAGGATG TGGACCGGAA GCCAGTGGGC GGAACAAAGA TGGACAAGGA CATCCTGGCG 
CGGACAATGC AACGGCTACG CAGTGGGGTG ATCCACAAAC AGGTGGCATC AGGGCGTGAG 
TTCAGAGGGT GCCCCTTCAA CAGCAAGCCC AAGTTCATCC ACTTCAAGGA CTTTGATGTG 
GGCAAAGTGT ACAAGAAGAA GATCACGCTG ATCAACGCCA CCTACACGAT TAACTACTGC 
AAGCTGGTGG GCGTGGATGA GAGCCTGCAG GACTTCATCC ACGTGGACTT TGACCCCCCA 
GGCCCACTAT CAGCCGGCAT GTCCTGCGAA GTGCTTGTCA CCTTCAAGCC CATGATAAAC 
AAAGATCTGG AAGGAAATGT CTCGTTTCTG GCTCAGACAG GCGGATTTTC TGTTCCACTG 
AAATGTTCAA CAAAGAAATG CTCTCTGTCC CTCGACAAGG AGCTCATCGA CTTTGGCAGC 
TACACAGTGG GTGAGACTGC AATCCGGACC ATCACGCTGA CCAACACTGG GGGCTTGGGC 
ACCAAGTTCA GATTTCTTCT GGCATCAGAG ACTTGTGAGA TGGACACCTC CCAGTCGGTC 
CTAAAATTAA GTAGTACCTT CACGTATGAA GACAAAAGTT TGTATGATAA ATTCACCACC 
AGTTTTTCTG AGCATCAGAT GGAGGGCAAT GAGTCATCTC CTACAGATGT ACCAAGCCAG 
AAGGAGTCTG GAAAGCTGGA CGTGAAGGAG CAGGAGGAGG GGCACCCCAT CGAATCAGAG 
GGCGTGACCT TGACCACCAT CATGACTATA CCTCTCAATG AGGAACAGAT GGAGTTCTCG 
CTGGGAGAGA CAACAGAGGG GGACATAGGC CCCTTCAGCT CCATCAAGGT GCCCGTCATC 
TTCACCCCGA TTGTCCCGGG AACGGTCCAG AACAGTTTCA AGGTTGTGTT TAAGAATCAG 
CAGTGCCCAA CTTTATATTT CAAAGTCGTC GGCGTGGCTA TTGATGTGCC TGTCTGGGTG 
CCAAAGCCCA ACGTAGACCT GAAGATCTGC ATGTACGATC GGCTGTACCA GGACTCGATC 
CTCGTCCACA CGCGGTCGAA GGCCGCGTTG CGCCTAAAGT TCGAGGTGTG CAAGGAGCTG 
CGGGGCCACG TGGAGCTCCT GCCTGAGACT GGCTACATCC AGGCCCTGTC GTCCTACTCA 
GTGCAGCTCA AGTTCCTGCC CAGACACTCC CTCCCAGAAG ATGCAGGGAA GTATTTTGAC 
AAGGAGACCA GGGTTCTGGA GGCCCCGATG ACTATCCGGG TGGCTGACCA GATCAAACCC 
GTGGGGTTTA CTGTCCATGC TGTAGTCACC ACCTCAGACC TGGAGCTCAG CCCCGCAGGG 
CTGGACTTTG GCTACTGTAC CATCTACGAG GCCATCAGGA CCCAAATCAG CCTCTGCAAT 
CATTCCCTCC TGCCCCAGGA GTTTGGGTTC GTTGGACTCC CCAAGTTTGT GGACATCCAG 
CCCAACGATG GGTTCGGGAC AATCCTGCCA CTGGAAACGC TACAGCTCGA TGTGATCTTT 
CAGCCCACCA AGGCCAAGGA GTACAACTTT GAGCTCATCT GCAAGTCAGA GATTAATCGG 
TGCTTCAAGC TGGCATGCCG GGCCGTGGGT GTCTACCCAC CCCTGGAGCT GTCCCACAAC 
CAGATCAAGT TTGCAGCCAC CTCCTTGTAC GACACATCCG TGGCCACTCT GTACGTCATC 
AACTCCCACC TGAGCATGAA CTCACTGACC CACTCAGTGC CCCGCATTGG CTCAGAGGAC 
GCTTTTCCCG TGGGGCCCAC GTCTTTTGAG TTCCTGCTGC CCCCAGATTC GCCCATCACC 
ATCTCACCCC TGGTGGGCAC CGTGTGGCCA GGAAAGAGGT GCCTGGTCCG AGTGGCCTTC 
CGGCCAGTGC TGCCCAGCGA GCTGATCCAC CAGGAGGCCT TCCAGGCCCT GAGCAGAAAA 
GCTGAGGTCA AATCGTCCCA AAAGGAGACG GTCACCCAGA GGAAGGACCA GCGGAGACCA 
TCTTTCTCCA TGCTGCGGCT GCAGAACCAA GACCGGCTGC TCCGGGCCTC AGCCTCCCAG 
CCCCCGGAGC TGCAGAAGGA GGACCTTGCA TCCAGCTCTC AAGAGTACCA GTCGGCACAG 
GCCTCCCTGA CCAGGTCTTT CCAGGGGAAG TTTGACAAGT TTGTGGTTCC CTGTGTTGTT 
GCTAGCGGCG ACACCAAAGA CAGGAAGGGG ACAGAACCGC TGAGCTTCAG TCCCCACAAC 
ACGCTGTACC TGGAGCTGTG GTGTCCAGCA GTGGCGCCGT CTATCGTGGT GACGTCCGAC 
AAAGGCAAGA CCATCATTAA CTTCGGCGAC ATTGCTGCAG GACACCGGGG CATAAAGAAG 
GTCTCCATCC AGAACATCTC CCCTGAGGAT CTCGACCTGG AGTACTCCGT GCTGAACCCC 
GATGGCCCCT TCGTCCTGCT GAACCCCACC ATCAGGTTGC CCTCTGGCGA GACCCAGGTC 
CTAGATCTGT CCTTCTCCCC ACGTGAGAGT GTCGTGGCCC AAGAAATACT GGACATCATC 
ACCAAGAAAG GCACGCTCTC CCTGGCCCTC ATGGGCACGG GTGTGGCGTC CACGATCACG 
TGCTCCATTG AAGGTGATGT CCTCAACATG GGCTACGTGA TCGCCAGAGA GTCTGTCTCC 
TTGGGCTTCA AGCTGCAGAA CAACTCCCCG CTGCCCATCA AGTTCTCCAT GCAGCTGGAC 
AGCCTCTCCT CCAGGAGCAG AGACCAGCAC CGCCTGCCAC AGTTCCTGGC CTCGCCTGAC 
CAGAGGACGG AAGTCGTGGG CACGCAGAAC TACAGTGGCC AGAGCGTGTT CAGCGTGGTC 
CCGGTCGAGG GCGTCATGGA GCCCGGCAAG GCTCAGGACT TCACTGTGAC CTTCAGCCCA 
GACCACGAGA GCCTATACTT CTCTGACCGG CTGCAGGTCG TGCTCTTTGA AAAGGCAGGA 
CTCCGACCCA GCCTGGACCC CTCCTCCCCA CCCGCATGTC CATTTGTCCA AGCCCTTTGG 
CTGCCTGAGC GGCCCCACAT TGGAGCTTGG TGCCTGGGGG GGCTCCACAT CAGGCTGGGG 
GTCATCAGGT GGAGAGACCA ATGTCTGGGG TCCCACAGGC CCACATCACA GCCCCGCCCC 
CTCCCAGAGC CCCACATGCA GGGTTGCAAA CCGCTGTGCT CCTGCCCCCA GAAAGTGTCC 
CACCACATCC TCCTGAAGGG CGCGGCCCGC GAGCACATGA TGTTCGTGGA GGGCGGAGAC 
CCCCTGGACG TGCCCGTGGA GTCCCTGACC GTGATCCCTG CCTTTGACCT GGAGCACAGA 
GAGGAAGCTG AGGAGCTAAA GCCCATCCTG GTGACCCTGG ACTACGCCCA GCTGGACCCA 
GACACACAGG CCCCACCTGC CACCCGAGAA CTGCAGGTGG GCTGCATCCG GACTACCCAG 
CTGTCCGCAA AGAAGACTGT TGAGTTCAGC ATGGACAACA TCGCAGCCCT GCAGCACAAG 
GGCTTCACCA TTGAGCCCTC CAGAGGCTCT GTGGAGCGCG GCCAGACCAA GGCCATCAGC 
ATCTCCTGGA CACCACCCAT CGACTTTGAT CCGGACCACC CACTCTTGGC ATCGGCCCTG 
CTGCAGCTCA GGGGGGATGT GAAGGAGACC TACAAGGTCC TCTTTGTAGC CCAGGTGGTG 
ACCGGTCCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024832558.1
CDS	416..5587
Translation	MEEDDKKLLDDKPLVEDDTEELEDPELVLDCVPREAEQKAGLGSSCPGIEQSKDKQKQRLTAQEKRQACLLRRDLSHLDQLHEEKDLFIQKTRGELRACRRRMELLVKQQESVAAEIAAERAANNMAAMGRLEAASRRLCSELKNEQDLQAQMMAVLQQSQSALWQIEVQEGQLEDARKSAQEEAAAARRGLQERAAKQLHREMGASGKAERNRLLRARRCVHLQKELGLRQQKLVEEAQKNHRKALRFLKASLGRIREQEQKEELETREHMRRRMDAVLALKNSISANRETLRKFQAWGQAKTELAEQKAQAEKKAILAQGGDAFKHLCHQRRRQELEAQNRAFEEEQKRRKQEIVSRLLKEEAVEDRRRPRPEPPRPAGQRSLRDQTWSYVATFFDGQSTEAPLCCPLPDVPGQRPGPAQEQIKLQETRSRFYQSSPLLTAISSESVQGDGSLGGEDTLAWPEIPGLWKEDYKPFQVSKEDVDRKPVGGTKMDKDILARTMQRLRSGVIHKQVASGREFRGCPFNSKPKFIHFKDFDVGKVYKKKITLINATYTINYCKLVGVDESLQDFIHVDFDPPGPLSAGMSCEVLVTFKPMINKDLEGNVSFLAQTGGFSVPLKCSTKKCSLSLDKELIDFGSYTVGETAIRTITLTNTGGLGTKFRFLLASETCEMDTSQSVLKLSSTFTYEDKSLYDKFTTSFSEHQMEGNESSPTDVPSQKESGKLDVKEQEEGHPIESEGVTLTTIMTIPLNEEQMEFSLGETTEGDIGPFSSIKVPVIFTPIVPGTVQNSFKVVFKNQQCPTLYFKVVGVAIDVPVWVPKPNVDLKICMYDRLYQDSILVHTRSKAALRLKFEVCKELRGHVELLPETGYIQALSSYSVQLKFLPRHSLPEDAGKYFDKETRVLEAPMTIRVADQIKPVGFTVHAVVTTSDLELSPAGLDFGYCTIYEAIRTQISLCNHSLLPQEFGFVGLPKFVDIQPNDGFGTILPLETLQLDVIFQPTKAKEYNFELICKSEINRCFKLACRAVGVYPPLELSHNQIKFAATSLYDTSVATLYVINSHLSMNSLTHSVPRIGSEDAFPVGPTSFEFLLPPDSPITISPLVGTVWPGKRCLVRVAFRPVLPSELIHQEAFQALSRKAEVKSSQKETVTQRKDQRRPSFSMLRLQNQDRLLRASASQPPELQKEDLASSSQEYQSAQASLTRSFQGKFDKFVVPCVVASGDTKDRKGTEPLSFSPHNTLYLELWCPAVAPSIVVTSDKGKTIINFGDIAAGHRGIKKVSIQNISPEDLDLEYSVLNPDGPFVLLNPTIRLPSGETQVLDLSFSPRESVVAQEILDIITKKGTLSLALMGTGVASTITCSIEGDVLNMGYVIARESVSLGFKLQNNSPLPIKFSMQLDSLSSRSRDQHRLPQFLASPDQRTEVVGTQNYSGQSVFSVVPVEGVMEPGKAQDFTVTFSPDHESLYFSDRLQVVLFEKAGLRPSLDPSSPPACPFVQALWLPERPHIGAWCLGGLHIRLGVIRWRDQCLGSHRPTSQPRPLPEPHMQGCKPLCSCPQKVSHHILLKGAAREHMMFVEGGDPLDVPVESLTVIPAFDLEHREEAEELKPILVTLDYAQLDPDTQAPPATRELQVGCIRTTQLSAKKTVEFSMDNIAALQHKGFTIEPSRGSVERGQTKAISISWTPPIDFDPDHPLLASALLQLRGDVKETYKVLFVAQVVTGP

Target ORF information:

RefSeq Version	XM_024976790.1
Organism	Bos taurus(cattle)
Definition	Bos taurus cilia and flagella associated protein 74 (CFAP74), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024976790.1

ORF Insert Sequence:

ATGGAAGAAG ACGACAAGAA GCTTTTGGAT GACAAGCCGC TCGTGGAAGA TGACACAGAG 
GAGCTGGAGG ACCCAGAGCT TGTGCTGGAC TGTGTCCCCA GGGAGGCTGA GCAGAAGGCC 
GGCCTGGGAA GCTCCTGTCC AGGAATAGAG CAGAGCAAGG ACAAGCAGAA GCAGAGGTTG 
ACTGCTCAGG AGAAAAGGCA GGCCTGCCTG CTGCGCAGGG ACTTGAGCCA CCTGGACCAG 
CTGCATGAAG AGAAAGACCT TTTCATCCAG AAGACAAGGG GGGAGCTACG TGCCTGCCGC 
AGGAGGATGG AACTCCTAGT CAAGCAGCAG GAGAGTGTGG CAGCCGAGAT AGCTGCAGAG 
AGGGCAGCCA ACAACATGGC AGCCATGGGC CGCCTGGAGG CAGCGTCGAG GCGCCTGTGC 
TCAGAGCTGA AGAACGAGCA GGACCTGCAG GCGCAGATGA TGGCGGTGCT GCAGCAGAGC 
CAGAGCGCCC TGTGGCAGAT TGAGGTCCAG GAGGGGCAGC TAGAGGACGC CCGCAAGTCG 
GCCCAAGAGG AGGCTGCAGC CGCCAGGAGA GGCCTTCAGG AGCGGGCAGC CAAGCAGCTG 
CACCGGGAGA TGGGGGCCTC GGGGAAGGCA GAGCGGAACA GGCTGCTGCG AGCCCGGAGA 
TGCGTGCACC TGCAGAAGGA GCTTGGACTC CGGCAGCAGA AGCTGGTGGA GGAAGCCCAG 
AAGAACCACC GGAAGGCCCT GAGGTTCCTG AAGGCCTCGT TGGGGAGGAT CCGTGAGCAG 
GAGCAGAAGG AGGAGCTGGA GACCCGAGAG CACATGCGCA GGCGCATGGA CGCGGTGCTG 
GCCCTGAAGA ACAGTATCTC CGCCAACCGG GAAACGCTCC GGAAATTTCA AGCATGGGGC 
CAGGCCAAGA CAGAGCTGGC AGAACAGAAG GCCCAGGCGG AGAAGAAGGC AATTCTGGCC 
CAAGGTGGGG ACGCCTTTAA GCACCTGTGC CATCAGCGCC GGCGCCAGGA GTTGGAGGCC 
CAGAACCGCG CCTTCGAGGA GGAGCAGAAG CGCAGGAAGC AGGAGATTGT GAGTCGGCTC 
TTGAAGGAAG AGGCTGTGGA GGACCGGAGG AGGCCACGGC CCGAGCCCCC GAGACCCGCA 
GGCCAGCGGA GCCTGAGGGA CCAGACCTGG AGCTACGTGG CCACCTTCTT CGATGGGCAG 
AGCACAGAGG CCCCGCTCTG CTGTCCCCTG CCTGATGTCC CTGGACAGAG GCCAGGTCCA 
GCCCAAGAGC AGATAAAGCT CCAGGAGACC AGGTCCAGAT TCTACCAGTC CTCTCCGCTG 
CTGACGGCCA TCTCCAGTGA GTCTGTCCAG GGGGACGGGA GCCTTGGCGG GGAGGACACA 
CTGGCTTGGC CGGAGATCCC CGGGCTCTGG AAGGAGGACT ACAAGCCTTT CCAGGTATCC 
AAGGAGGATG TGGACCGGAA GCCAGTGGGC GGAACAAAGA TGGACAAGGA CATCCTGGCG 
CGGACAATGC AACGGCTACG CAGTGGGGTG ATCCACAAAC AGGTGGCATC AGGGCGTGAG 
TTCAGAGGGT GCCCCTTCAA CAGCAAGCCC AAGTTCATCC ACTTCAAGGA CTTTGATGTG 
GGCAAAGTGT ACAAGAAGAA GATCACGCTG ATCAACGCCA CCTACACGAT TAACTACTGC 
AAGCTGGTGG GCGTGGATGA GAGCCTGCAG GACTTCATCC ACGTGGACTT TGACCCCCCA 
GGCCCACTAT CAGCCGGCAT GTCCTGCGAA GTGCTTGTCA CCTTCAAGCC CATGATAAAC 
AAAGATCTGG AAGGAAATGT CTCGTTTCTG GCTCAGACAG GCGGATTTTC TGTTCCACTG 
AAATGTTCAA CAAAGAAATG CTCTCTGTCC CTCGACAAGG AGCTCATCGA CTTTGGCAGC 
TACACAGTGG GTGAGACTGC AATCCGGACC ATCACGCTGA CCAACACTGG GGGCTTGGGC 
ACCAAGTTCA GATTTCTTCT GGCATCAGAG ACTTGTGAGA TGGACACCTC CCAGTCGGTC 
CTAAAATTAA GTAGTACCTT CACGTATGAA GACAAAAGTT TGTATGATAA ATTCACCACC 
AGTTTTTCTG AGCATCAGAT GGAGGGCAAT GAGTCATCTC CTACAGATGT ACCAAGCCAG 
AAGGAGTCTG GAAAGCTGGA CGTGAAGGAG CAGGAGGAGG GGCACCCCAT CGAATCAGAG 
GGCGTGACCT TGACCACCAT CATGACTATA CCTCTCAATG AGGAACAGAT GGAGTTCTCG 
CTGGGAGAGA CAACAGAGGG GGACATAGGC CCCTTCAGCT CCATCAAGGT GCCCGTCATC 
TTCACCCCGA TTGTCCCGGG AACGGTCCAG AACAGTTTCA AGGTTGTGTT TAAGAATCAG 
CAGTGCCCAA CTTTATATTT CAAAGTCGTC GGCGTGGCTA TTGATGTGCC TGTCTGGGTG 
CCAAAGCCCA ACGTAGACCT GAAGATCTGC ATGTACGATC GGCTGTACCA GGACTCGATC 
CTCGTCCACA CGCGGTCGAA GGCCGCGTTG CGCCTAAAGT TCGAGGTGTG CAAGGAGCTG 
CGGGGCCACG TGGAGCTCCT GCCTGAGACT GGCTACATCC AGGCCCTGTC GTCCTACTCA 
GTGCAGCTCA AGTTCCTGCC CAGACACTCC CTCCCAGAAG ATGCAGGGAA GTATTTTGAC 
AAGGAGACCA GGGTTCTGGA GGCCCCGATG ACTATCCGGG TGGCTGACCA GATCAAACCC 
GTGGGGTTTA CTGTCCATGC TGTAGTCACC ACCTCAGACC TGGAGCTCAG CCCCGCAGGG 
CTGGACTTTG GCTACTGTAC CATCTACGAG GCCATCAGGA CCCAAATCAG CCTCTGCAAT 
CATTCCCTCC TGCCCCAGGA GTTTGGGTTC GTTGGACTCC CCAAGTTTGT GGACATCCAG 
CCCAACGATG GGTTCGGGAC AATCCTGCCA CTGGAAACGC TACAGCTCGA TGTGATCTTT 
CAGCCCACCA AGGCCAAGGA GTACAACTTT GAGCTCATCT GCAAGTCAGA GATTAATCGG 
TGCTTCAAGC TGGCATGCCG GGCCGTGGGT GTCTACCCAC CCCTGGAGCT GTCCCACAAC 
CAGATCAAGT TTGCAGCCAC CTCCTTGTAC GACACATCCG TGGCCACTCT GTACGTCATC 
AACTCCCACC TGAGCATGAA CTCACTGACC CACTCAGTGC CCCGCATTGG CTCAGAGGAC 
GCTTTTCCCG TGGGGCCCAC GTCTTTTGAG TTCCTGCTGC CCCCAGATTC GCCCATCACC 
ATCTCACCCC TGGTGGGCAC CGTGTGGCCA GGAAAGAGGT GCCTGGTCCG AGTGGCCTTC 
CGGCCAGTGC TGCCCAGCGA GCTGATCCAC CAGGAGGCCT TCCAGGCCCT GAGCAGAAAA 
GCTGAGGTCA AATCGTCCCA AAAGGAGACG GTCACCCAGA GGAAGGACCA GCGGAGACCA 
TCTTTCTCCA TGCTGCGGCT GCAGAACCAA GACCGGCTGC TCCGGGCCTC AGCCTCCCAG 
CCCCCGGAGC TGCAGAAGGA GGACCTTGCA TCCAGCTCTC AAGAGTACCA GTCGGCACAG 
GCCTCCCTGA CCAGGTCTTT CCAGGGGAAG TTTGACAAGT TTGTGGTTCC CTGTGTTGTT 
GCTAGCGGCG ACACCAAAGA CAGGAAGGGG ACAGAACCGC TGAGCTTCAG TCCCCACAAC 
ACGCTGTACC TGGAGCTGTG GTGTCCAGCA GTGGCGCCGT CTATCGTGGT GACGTCCGAC 
AAAGGCAAGA CCATCATTAA CTTCGGCGAC ATTGCTGCAG GACACCGGGG CATAAAGAAG 
GTCTCCATCC AGAACATCTC CCCTGAGGAT CTCGACCTGG AGTACTCCGT GCTGAACCCC 
GATGGCCCCT TCGTCCTGCT GAACCCCACC ATCAGGTTGC CCTCTGGCGA GACCCAGGTC 
CTAGATCTGT CCTTCTCCCC ACGTGAGAGT GTCGTGGCCC AAGAAATACT GGACATCATC 
ACCAAGAAAG GCACGCTCTC CCTGGCCCTC ATGGGCACGG GTGTGGCGTC CACGATCACG 
TGCTCCATTG AAGGTGATGT CCTCAACATG GGCTACGTGA TCGCCAGAGA GTCTGTCTCC 
TTGGGCTTCA AGCTGCAGAA CAACTCCCCG CTGCCCATCA AGTTCTCCAT GCAGCTGGAC 
AGCCTCTCCT CCAGGAGCAG AGACCAGCAC CGCCTGCCAC AGTTCCTGGC CTCGCCTGAC 
CAGAGGACGG AAGTCGTGGG CACGCAGAAC TACAGTGGCC AGAGCGTGTT CAGCGTGGTC 
CCGGTCGAGG GCGTCATGGA GCCCGGCAAG GCTCAGGACT TCACTGTGAC CTTCAGCCCA 
GACCACGAGA GCCTATACTT CTCTGACCGG CTGCAGGTCG TGCTCTTTGA AAAGGCAGGA 
CTCCGACCCA GCCTGGACCC CTCCTCCCCA CCCGCATGTC CATTTGTCCA AGCCCTTTGG 
CTGCCTGAGC GGCCCCACAT TGGAGCTTGG TGCCTGGGGG GGCTCCACAT CAGGCTGGGG 
GTCATCAGGT GGAGAGACCA ATGTCTGGGG TCCCACAGGC CCACATCACA GCCCCGCCCC 
CTCCCAGAGC CCCACATGCA GGGTTGCAAA CCGCTGTGCT CCTGCCCCCA GAAAGTGTCC 
CACCACATCC TCCTGAAGGG CGCGGCCCGC GAGCACATGA TGTTCGTGGA GGGCGGAGAC 
CCCCTGGACG TGCCCGTGGA GTCCCTGACC GTGATCCCTG CCTTTGACCT GGAGCACAGA 
GAGGAAGCTG AGGAGCTAAA GCCCATCCTG GTGACCCTGG ACTACGCCCA GCTGGACCCA 
GACACACAGG CCCCACCTGC CACCCGAGAA CTGCAGGTGG GCTGCATCCG GACTACCCAG 
CTGTCCGCAA AGAAGACTGT TGAGTTCAGC ATGGACAACA TCGCAGCCCT GCAGCACAAG 
GGCTTCACCA TTGAGCCCTC CAGAGGCTCT GTGGAGCGCG GCCAGACCAA GGCCATCAGC 
ATCTCCTGGA CACCACCCAT CGACTTTGAT CCGGACCACC CACTCTTGGC ATCGGCCCTG 
CTGCAGCTCA GGGGGGATGT GAAGGAGACC TACAAGGTCC TCTTTGTAGC CCAGGTGGTG 
ACCGGTCCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CFAP74 ( XM_024976792.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024832560.1 Bos taurus cilia and flagella associated protein 74 (CFAP74), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa56328
Clone ID Related Accession (Same CDS sequence)	XM_024976792.1
Accession Version	XM_024976792.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5169bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	cilia- and flagella-associated protein 74 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAAG ACGACAAGAA GCTTTTGGAT GACAAGCCGC TCGTGGAAGA TGACACAGAG 
GAGCTGGAGG ACCCAGAGCT TGTGCTGGAC TGTGTCCCCA GGGAGGCTGA GCAGAAGGCC 
GGCCTGGGAA GCTCCTGTCC AGGAATAGAG CAGAGCAAGG ACAAGCAGAA GCAGAGGTTG 
ACTGCTCAGG AGAAAAGGCA GGCCTGCCTG CTGCGCAGGG ACTTGAGCCA CCTGGACCAG 
CTGCATGAAG AGAAAGACCT TTTCATCCAG AAGACAAGGG GGGAGCTACG TGCCTGCCGC 
AGGAGGATGG AACTCCTAGT CAAGCAGCAG GAGAGTGTGG CAGCCGAGAT AGCTGCAGAG 
AGGGCAGCCA ACAACATGGC AGCCATGGGC CGCCTGGAGG CAGCGTCGAG GCGCCTGTGC 
TCAGAGCTGA AGAACGAGCA GGACCTGCAG GCGCAGATGA TGGCGGTGCT GCAGCAGAGC 
CAGAGCGCCC TGTGGCAGAT TGAGGTCCAG GAGGGGCAGC TAGAGGACGC CCGCAAGTCG 
GCCCAAGAGG AGGCTGCAGC CGCCAGGAGA GGCCTTCAGG AGCGGGCAGC CAAGCAGCTG 
CACCGGGAGA TGGGGGCCTC GGGGAAGGCA GAGCGGAACA GGCTGCTGCG AGCCCGGAGA 
TGCGTGCACC TGCAGAAGGA GCTTGGACTC CGGCAGCAGA AGCTGGTGGA GGAAGCCCAG 
AAGAACCACC GGAAGGCCCT GAGGTTCCTG AAGGCCTCGT TGGGGAGGAT CCGTGAGCAG 
GAGCAGAAGG AGGAGCTGGA GACCCGAGAG CACATGCGCA GGCGCATGGA CGCGGTGCTG 
GCCCTGAAGA ACAGTATCTC CGCCAACCGG GAAACGCTCC GGAAATTTCA AGCATGGGGC 
CAGGCCAAGA CAGAGCTGGC AGAACAGAAG GCCCAGGCGG AGAAGAAGGC AATTCTGGCC 
CAAGGTGGGG ACGCCTTTAA GCACCTGTGC CATCAGCGCC GGCGCCAGGA GTTGGAGGCC 
CAGAACCGCG CCTTCGAGGA GGAGCAGAAG CGCAGGAAGC AGGAGATTGT GAGTCGGCTC 
TTGAAGGAAG AGGCTGTGGA GGACCGGAGG AGGCCACGGC CCGAGCCCCC GAGACCCGCA 
GGCCAGCGGA GCCTGAGGGA CCAGACCTGG AGCTACGTGG CCACCTTCTT CGATGGGCAG 
AGCACAGAGG CCCCGCTCTG CTGTCCCCTG CCTGATGTCC CTGGACAGAG GCCAGGTCCA 
GCCCAAGAGC AGATAAAGCT CCAGGAGACC AGGTCCAGAT TCTACCAGTC CTCTCCGCTG 
CTGACGGCCA TCTCCAGTGA GTCTGTCCAG GGGGACGGGA GCCTTGGCGG GGAGGACACA 
CTGGCTTGGC CGGAGATCCC CGGGCTCTGG AAGGAGGACT ACAAGCCTTT CCAGGTATCC 
AAGGAGGATG TGGACCGGAA GCCAGTGGGC GGAACAAAGA TGGACAAGGA CATCCTGGCG 
CGGACAATGC AACGGCTACG CAGTGGGGTG ATCCACAAAC AGGTGGCATC AGGGCGTGAG 
TTCAGAGGGT GCCCCTTCAA CAGCAAGCCC AAGTTCATCC ACTTCAAGGA CTTTGATGTG 
GGCAAAGTGT ACAAGAAGAA GATCACGCTG ATCAACGCCA CCTACACGAT TAACTACTGC 
AAGCTGGTGG GCGTGGATGA GAGCCTGCAG GACTTCATCC ACGTGGACTT TGACCCCCCA 
GGCCCACTAT CAGCCGGCAT GTCCTGCGAA GTGCTTGTCA CCTTCAAGCC CATGATAAAC 
AAAGATCTGG AAGGAAATGT CTCGTTTCTG GCTCAGACAG GCGGATTTTC TGTTCCACTG 
AAATGTTCAA CAAAGAAATG CTCTCTGTCC CTCGACAAGG AGCTCATCGA CTTTGGCAGC 
TACACAGTGG GTGAGACTGC AATCCGGACC ATCACGCTGA CCAACACTGG GGGCTTGGGC 
ACCAAGTTCA GATTTCTTCT GGCATCAGAG ACTTGTGAGA TGGACACCTC CCAGTCGGTC 
CTAAAATTAA GTAGTACCTT CACGTATGAA GACAAAAGTT TGTATGATAA ATTCACCACC 
AGTTTTTCTG AGCATCAGAT GGAGGGCAAT GAGTCATCTC CTACAGATGT ACCAAGCCAG 
AAGGAGTCTG GAAAGCTGGA CGTGAAGGAG CAGGAGGAGG GGCACCCCAT CGAATCAGAG 
GGCGTGACCT TGACCACCAT CATGACTATA CCTCTCAATG AGGAACAGAT GGAGTTCTCG 
CTGGGAGAGA CAACAGAGGG GGACATAGGC CCCTTCAGCT CCATCAAGGT GCCCGTCATC 
TTCACCCCGA TTGTCCCGGG AACGGTCCAG AACAGTTTCA AGGTTGTGTT TAAGAATCAG 
CAGTGCCCAA CTTTATATTT CAAAGTCGTC GGCGTGGCTA TTGATGTGCC TGTCTGGGTG 
CCAAAGCCCA ACGTAGACCT GAAGATCTGC ATGTACGATC GGCTGTACCA GGACTCGATC 
CTCGTCCACA CGCGGTCGAA GGCCGCGTTG CGCCTAAAGT TCGAGGTGTG CAAGGAGCTG 
CGGGGCCACG TGGAGCTCCT GCCTGAGACT GGCTACATCC AGGCCCTGTC GTCCTACTCA 
GTGCAGCTCA AGTTCCTGCC CAGACACTCC CTCCCAGAAG ATGCAGGGAA GTATTTTGAC 
AAGGAGACCA GGGTTCTGGA GGCCCCGATG ACTATCCGGG TGGCTGACCA GATCAAACCC 
GTGGGGTTTA CTGTCCATGC TGTAGTCACC ACCTCAGACC TGGAGCTCAG CCCCGCAGGG 
CTGGACTTTG GCTACTGTAC CATCTACGAG GCCATCAGGA CCCAAATCAG CCTCTGCAAT 
CATTCCCTCC TGCCCCAGGA GTTTGGGTTC GTTGGACTCC CCAAGTTTGT GGACATCCAG 
CCCAACGATG GGTTCGGGAC AATCCTGCCA CTGGAAACGC TACAGCTCGA TGTGATCTTT 
CAGCCCACCA AGGCCAAGGA GTACAACTTT GAGCTCATCT GCAAGTCAGA GATTAATCGG 
TGCTTCAAGC TGGCATGCCG GGCCGTGGGT GTCTACCCAC CCCTGGAGCT GTCCCACAAC 
CAGATCAAGT TTGCAGCCAC CTCCTTGTAC GACACATCCG TGGCCACTCT GTACGTCATC 
AACTCCCACC TGAGCATGAA CTCACTGACC CACTCAGTGC CCCGCATTGG CTCAGAGGAC 
GCTTTTCCCG TGGGGCCCAC GTCTTTTGAG TTCCTGCTGC CCCCAGATTC GCCCATCACC 
ATCTCACCCC TGGTGGGCAC CGTGTGGCCA GGAAAGAGGT GCCTGGTCCG AGTGGCCTTC 
CGGCCAGTGC TGCCCAGCGA GCTGATCCAC CAGGAGGCCT TCCAGGCCCT GAGCAGAAAA 
GCTGAGGTCA AATCGTCCCA AAAGGAGACG GTCACCCAGA GGAAGGACCA GCGGAGACCA 
TCTTTCTCCA TGCTGCGGCT GCAGAACCAA GACCGGCTGC TCCGGGCCTC AGCCTCCCAG 
CCCCCGGAGC TGCAGAAGGA GGACCTTGCA TCCAGCTCTC AAGAGTACCA GTCGGCACAG 
GCCTCCCTGA CCAGGTCTTT CCAGGGGAAG TTTGACAAGT TTGTGGTTCC CTGTGTTGTT 
GCTAGCGGCG ACACCAAAGA CAGGAAGGGG ACAGAACCGC TGAGCTTCAG TCCCCACAAC 
ACGCTGTACC TGGAGCTGTG GTGTCCAGCA GTGGCGCCGT CTATCGTGGT GACGTCCGAC 
AAAGGCAAGA CCATCATTAA CTTCGGCGAC ATTGCTGCAG GACACCGGGG CATAAAGAAG 
GTCTCCATCC AGAACATCTC CCCTGAGGAT CTCGACCTGG AGTACTCCGT GCTGAACCCC 
GATGGCCCCT TCGTCCTGCT GAACCCCACC ATCAGGTTGC CCTCTGGCGA GACCCAGGTC 
CTAGATCTGT CCTTCTCCCC ACGTGAGAGT GTCGTGGCCC AAGAAATACT GGACATCATC 
ACCAAGAAAG GCACGCTCTC CCTGGCCCTC ATGGGCACGG GTGTGGCGTC CACGATCACG 
TGCTCCATTG AAGGTGATGT CCTCAACATG GGCTACGTGA TCGCCAGAGA GTCTGTCTCC 
TTGGGCTTCA AGCTGCAGAA CAACTCCCCG CTGCCCATCA AGTTCTCCAT GCAGCTGGAC 
AGCCTCTCCT CCAGGAGCAG AGACCAGCAC CGCCTGCCAC AGTTCCTGGC CTCGCCTGAC 
CAGAGGACGG AAGTCGTGGG CACGCAGAAC TACAGTGGCC AGAGCGTGTT CAGCGTGGTC 
CCGGTCGAGG GCGTCATGGA GCCCGGCAAG GCTCAGGACT TCACTGTGAC CTTCAGCCCA 
GACCACGAGA GCCTATACTT CTCTGACCGG CTGCAGGTCG TGCTCTTTGA AAAGGCAGGA 
CTCCGACCCA GCCTGGACCC CTCCTCCCCA CCCGCATGTC CATTTGTCCA AGCCCTTTGG 
CTGCCTGAGC GGCCCCACAT TGGAGCTTGG TGCCTGGGGG GGCTCCACAT CAGGCTGGGG 
GTCATCAGGT GGAGAGACCA ATGTCTGGGG TCCCACAGGC CCACATCACA GCCCCGCCCC 
CTCCCAGAGC CCCACATGCA GGGTTGCAAA CCGCTGTGCT CCTGCCCCCA GAAAGTGTCC 
CACCACATCC TCCTGAAGGG CGCGGCCCGC GAGCACATGA TGTTCGTGGA GGGCGGAGAC 
CCCCTGGACG TGCCCGTGGA GTCCCTGACC GTGATCCCTG CCTTTGACCT GGAGCACAGA 
GAGGCTGAGG AGCTAAAGCC CATCCTGGTG ACCCTGGACT ACGCCCAGCT GGACCCAGAC 
ACACAGGCCC CACCTGCCAC CCGAGAACTG CAGGTGGGCT GCATCCGGAC TACCCAGCTG 
TCCGCAAAGA AGACTGTTGA GTTCAGCATG GACAACATCG CAGCCCTGCA GCACAAGGGC 
TTCACCATTG AGCCCTCCAG AGGCTCTGTG GAGCGCGGCC AGACCAAGGC CATCAGCATC 
TCCTGGACAC CACCCATCGA CTTTGATCCG GACCACCCAC TCTTGGCATC GGCCCTGCTG 
CAGCTCAGGG GGGATGTGAA GGAGACCTAC AAGGTCCTCT TTGTAGCCCA GGTGGTGACC 
GGTCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024832560.1
CDS	417..5585
Translation	MEEDDKKLLDDKPLVEDDTEELEDPELVLDCVPREAEQKAGLGSSCPGIEQSKDKQKQRLTAQEKRQACLLRRDLSHLDQLHEEKDLFIQKTRGELRACRRRMELLVKQQESVAAEIAAERAANNMAAMGRLEAASRRLCSELKNEQDLQAQMMAVLQQSQSALWQIEVQEGQLEDARKSAQEEAAAARRGLQERAAKQLHREMGASGKAERNRLLRARRCVHLQKELGLRQQKLVEEAQKNHRKALRFLKASLGRIREQEQKEELETREHMRRRMDAVLALKNSISANRETLRKFQAWGQAKTELAEQKAQAEKKAILAQGGDAFKHLCHQRRRQELEAQNRAFEEEQKRRKQEIVSRLLKEEAVEDRRRPRPEPPRPAGQRSLRDQTWSYVATFFDGQSTEAPLCCPLPDVPGQRPGPAQEQIKLQETRSRFYQSSPLLTAISSESVQGDGSLGGEDTLAWPEIPGLWKEDYKPFQVSKEDVDRKPVGGTKMDKDILARTMQRLRSGVIHKQVASGREFRGCPFNSKPKFIHFKDFDVGKVYKKKITLINATYTINYCKLVGVDESLQDFIHVDFDPPGPLSAGMSCEVLVTFKPMINKDLEGNVSFLAQTGGFSVPLKCSTKKCSLSLDKELIDFGSYTVGETAIRTITLTNTGGLGTKFRFLLASETCEMDTSQSVLKLSSTFTYEDKSLYDKFTTSFSEHQMEGNESSPTDVPSQKESGKLDVKEQEEGHPIESEGVTLTTIMTIPLNEEQMEFSLGETTEGDIGPFSSIKVPVIFTPIVPGTVQNSFKVVFKNQQCPTLYFKVVGVAIDVPVWVPKPNVDLKICMYDRLYQDSILVHTRSKAALRLKFEVCKELRGHVELLPETGYIQALSSYSVQLKFLPRHSLPEDAGKYFDKETRVLEAPMTIRVADQIKPVGFTVHAVVTTSDLELSPAGLDFGYCTIYEAIRTQISLCNHSLLPQEFGFVGLPKFVDIQPNDGFGTILPLETLQLDVIFQPTKAKEYNFELICKSEINRCFKLACRAVGVYPPLELSHNQIKFAATSLYDTSVATLYVINSHLSMNSLTHSVPRIGSEDAFPVGPTSFEFLLPPDSPITISPLVGTVWPGKRCLVRVAFRPVLPSELIHQEAFQALSRKAEVKSSQKETVTQRKDQRRPSFSMLRLQNQDRLLRASASQPPELQKEDLASSSQEYQSAQASLTRSFQGKFDKFVVPCVVASGDTKDRKGTEPLSFSPHNTLYLELWCPAVAPSIVVTSDKGKTIINFGDIAAGHRGIKKVSIQNISPEDLDLEYSVLNPDGPFVLLNPTIRLPSGETQVLDLSFSPRESVVAQEILDIITKKGTLSLALMGTGVASTITCSIEGDVLNMGYVIARESVSLGFKLQNNSPLPIKFSMQLDSLSSRSRDQHRLPQFLASPDQRTEVVGTQNYSGQSVFSVVPVEGVMEPGKAQDFTVTFSPDHESLYFSDRLQVVLFEKAGLRPSLDPSSPPACPFVQALWLPERPHIGAWCLGGLHIRLGVIRWRDQCLGSHRPTSQPRPLPEPHMQGCKPLCSCPQKVSHHILLKGAAREHMMFVEGGDPLDVPVESLTVIPAFDLEHREAEELKPILVTLDYAQLDPDTQAPPATRELQVGCIRTTQLSAKKTVEFSMDNIAALQHKGFTIEPSRGSVERGQTKAISISWTPPIDFDPDHPLLASALLQLRGDVKETYKVLFVAQVVTGP

Target ORF information:

RefSeq Version	XM_024976792.1
Organism	Bos taurus(cattle)
Definition	Bos taurus cilia and flagella associated protein 74 (CFAP74), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024976792.1

ORF Insert Sequence:

ATGGAAGAAG ACGACAAGAA GCTTTTGGAT GACAAGCCGC TCGTGGAAGA TGACACAGAG 
GAGCTGGAGG ACCCAGAGCT TGTGCTGGAC TGTGTCCCCA GGGAGGCTGA GCAGAAGGCC 
GGCCTGGGAA GCTCCTGTCC AGGAATAGAG CAGAGCAAGG ACAAGCAGAA GCAGAGGTTG 
ACTGCTCAGG AGAAAAGGCA GGCCTGCCTG CTGCGCAGGG ACTTGAGCCA CCTGGACCAG 
CTGCATGAAG AGAAAGACCT TTTCATCCAG AAGACAAGGG GGGAGCTACG TGCCTGCCGC 
AGGAGGATGG AACTCCTAGT CAAGCAGCAG GAGAGTGTGG CAGCCGAGAT AGCTGCAGAG 
AGGGCAGCCA ACAACATGGC AGCCATGGGC CGCCTGGAGG CAGCGTCGAG GCGCCTGTGC 
TCAGAGCTGA AGAACGAGCA GGACCTGCAG GCGCAGATGA TGGCGGTGCT GCAGCAGAGC 
CAGAGCGCCC TGTGGCAGAT TGAGGTCCAG GAGGGGCAGC TAGAGGACGC CCGCAAGTCG 
GCCCAAGAGG AGGCTGCAGC CGCCAGGAGA GGCCTTCAGG AGCGGGCAGC CAAGCAGCTG 
CACCGGGAGA TGGGGGCCTC GGGGAAGGCA GAGCGGAACA GGCTGCTGCG AGCCCGGAGA 
TGCGTGCACC TGCAGAAGGA GCTTGGACTC CGGCAGCAGA AGCTGGTGGA GGAAGCCCAG 
AAGAACCACC GGAAGGCCCT GAGGTTCCTG AAGGCCTCGT TGGGGAGGAT CCGTGAGCAG 
GAGCAGAAGG AGGAGCTGGA GACCCGAGAG CACATGCGCA GGCGCATGGA CGCGGTGCTG 
GCCCTGAAGA ACAGTATCTC CGCCAACCGG GAAACGCTCC GGAAATTTCA AGCATGGGGC 
CAGGCCAAGA CAGAGCTGGC AGAACAGAAG GCCCAGGCGG AGAAGAAGGC AATTCTGGCC 
CAAGGTGGGG ACGCCTTTAA GCACCTGTGC CATCAGCGCC GGCGCCAGGA GTTGGAGGCC 
CAGAACCGCG CCTTCGAGGA GGAGCAGAAG CGCAGGAAGC AGGAGATTGT GAGTCGGCTC 
TTGAAGGAAG AGGCTGTGGA GGACCGGAGG AGGCCACGGC CCGAGCCCCC GAGACCCGCA 
GGCCAGCGGA GCCTGAGGGA CCAGACCTGG AGCTACGTGG CCACCTTCTT CGATGGGCAG 
AGCACAGAGG CCCCGCTCTG CTGTCCCCTG CCTGATGTCC CTGGACAGAG GCCAGGTCCA 
GCCCAAGAGC AGATAAAGCT CCAGGAGACC AGGTCCAGAT TCTACCAGTC CTCTCCGCTG 
CTGACGGCCA TCTCCAGTGA GTCTGTCCAG GGGGACGGGA GCCTTGGCGG GGAGGACACA 
CTGGCTTGGC CGGAGATCCC CGGGCTCTGG AAGGAGGACT ACAAGCCTTT CCAGGTATCC 
AAGGAGGATG TGGACCGGAA GCCAGTGGGC GGAACAAAGA TGGACAAGGA CATCCTGGCG 
CGGACAATGC AACGGCTACG CAGTGGGGTG ATCCACAAAC AGGTGGCATC AGGGCGTGAG 
TTCAGAGGGT GCCCCTTCAA CAGCAAGCCC AAGTTCATCC ACTTCAAGGA CTTTGATGTG 
GGCAAAGTGT ACAAGAAGAA GATCACGCTG ATCAACGCCA CCTACACGAT TAACTACTGC 
AAGCTGGTGG GCGTGGATGA GAGCCTGCAG GACTTCATCC ACGTGGACTT TGACCCCCCA 
GGCCCACTAT CAGCCGGCAT GTCCTGCGAA GTGCTTGTCA CCTTCAAGCC CATGATAAAC 
AAAGATCTGG AAGGAAATGT CTCGTTTCTG GCTCAGACAG GCGGATTTTC TGTTCCACTG 
AAATGTTCAA CAAAGAAATG CTCTCTGTCC CTCGACAAGG AGCTCATCGA CTTTGGCAGC 
TACACAGTGG GTGAGACTGC AATCCGGACC ATCACGCTGA CCAACACTGG GGGCTTGGGC 
ACCAAGTTCA GATTTCTTCT GGCATCAGAG ACTTGTGAGA TGGACACCTC CCAGTCGGTC 
CTAAAATTAA GTAGTACCTT CACGTATGAA GACAAAAGTT TGTATGATAA ATTCACCACC 
AGTTTTTCTG AGCATCAGAT GGAGGGCAAT GAGTCATCTC CTACAGATGT ACCAAGCCAG 
AAGGAGTCTG GAAAGCTGGA CGTGAAGGAG CAGGAGGAGG GGCACCCCAT CGAATCAGAG 
GGCGTGACCT TGACCACCAT CATGACTATA CCTCTCAATG AGGAACAGAT GGAGTTCTCG 
CTGGGAGAGA CAACAGAGGG GGACATAGGC CCCTTCAGCT CCATCAAGGT GCCCGTCATC 
TTCACCCCGA TTGTCCCGGG AACGGTCCAG AACAGTTTCA AGGTTGTGTT TAAGAATCAG 
CAGTGCCCAA CTTTATATTT CAAAGTCGTC GGCGTGGCTA TTGATGTGCC TGTCTGGGTG 
CCAAAGCCCA ACGTAGACCT GAAGATCTGC ATGTACGATC GGCTGTACCA GGACTCGATC 
CTCGTCCACA CGCGGTCGAA GGCCGCGTTG CGCCTAAAGT TCGAGGTGTG CAAGGAGCTG 
CGGGGCCACG TGGAGCTCCT GCCTGAGACT GGCTACATCC AGGCCCTGTC GTCCTACTCA 
GTGCAGCTCA AGTTCCTGCC CAGACACTCC CTCCCAGAAG ATGCAGGGAA GTATTTTGAC 
AAGGAGACCA GGGTTCTGGA GGCCCCGATG ACTATCCGGG TGGCTGACCA GATCAAACCC 
GTGGGGTTTA CTGTCCATGC TGTAGTCACC ACCTCAGACC TGGAGCTCAG CCCCGCAGGG 
CTGGACTTTG GCTACTGTAC CATCTACGAG GCCATCAGGA CCCAAATCAG CCTCTGCAAT 
CATTCCCTCC TGCCCCAGGA GTTTGGGTTC GTTGGACTCC CCAAGTTTGT GGACATCCAG 
CCCAACGATG GGTTCGGGAC AATCCTGCCA CTGGAAACGC TACAGCTCGA TGTGATCTTT 
CAGCCCACCA AGGCCAAGGA GTACAACTTT GAGCTCATCT GCAAGTCAGA GATTAATCGG 
TGCTTCAAGC TGGCATGCCG GGCCGTGGGT GTCTACCCAC CCCTGGAGCT GTCCCACAAC 
CAGATCAAGT TTGCAGCCAC CTCCTTGTAC GACACATCCG TGGCCACTCT GTACGTCATC 
AACTCCCACC TGAGCATGAA CTCACTGACC CACTCAGTGC CCCGCATTGG CTCAGAGGAC 
GCTTTTCCCG TGGGGCCCAC GTCTTTTGAG TTCCTGCTGC CCCCAGATTC GCCCATCACC 
ATCTCACCCC TGGTGGGCAC CGTGTGGCCA GGAAAGAGGT GCCTGGTCCG AGTGGCCTTC 
CGGCCAGTGC TGCCCAGCGA GCTGATCCAC CAGGAGGCCT TCCAGGCCCT GAGCAGAAAA 
GCTGAGGTCA AATCGTCCCA AAAGGAGACG GTCACCCAGA GGAAGGACCA GCGGAGACCA 
TCTTTCTCCA TGCTGCGGCT GCAGAACCAA GACCGGCTGC TCCGGGCCTC AGCCTCCCAG 
CCCCCGGAGC TGCAGAAGGA GGACCTTGCA TCCAGCTCTC AAGAGTACCA GTCGGCACAG 
GCCTCCCTGA CCAGGTCTTT CCAGGGGAAG TTTGACAAGT TTGTGGTTCC CTGTGTTGTT 
GCTAGCGGCG ACACCAAAGA CAGGAAGGGG ACAGAACCGC TGAGCTTCAG TCCCCACAAC 
ACGCTGTACC TGGAGCTGTG GTGTCCAGCA GTGGCGCCGT CTATCGTGGT GACGTCCGAC 
AAAGGCAAGA CCATCATTAA CTTCGGCGAC ATTGCTGCAG GACACCGGGG CATAAAGAAG 
GTCTCCATCC AGAACATCTC CCCTGAGGAT CTCGACCTGG AGTACTCCGT GCTGAACCCC 
GATGGCCCCT TCGTCCTGCT GAACCCCACC ATCAGGTTGC CCTCTGGCGA GACCCAGGTC 
CTAGATCTGT CCTTCTCCCC ACGTGAGAGT GTCGTGGCCC AAGAAATACT GGACATCATC 
ACCAAGAAAG GCACGCTCTC CCTGGCCCTC ATGGGCACGG GTGTGGCGTC CACGATCACG 
TGCTCCATTG AAGGTGATGT CCTCAACATG GGCTACGTGA TCGCCAGAGA GTCTGTCTCC 
TTGGGCTTCA AGCTGCAGAA CAACTCCCCG CTGCCCATCA AGTTCTCCAT GCAGCTGGAC 
AGCCTCTCCT CCAGGAGCAG AGACCAGCAC CGCCTGCCAC AGTTCCTGGC CTCGCCTGAC 
CAGAGGACGG AAGTCGTGGG CACGCAGAAC TACAGTGGCC AGAGCGTGTT CAGCGTGGTC 
CCGGTCGAGG GCGTCATGGA GCCCGGCAAG GCTCAGGACT TCACTGTGAC CTTCAGCCCA 
GACCACGAGA GCCTATACTT CTCTGACCGG CTGCAGGTCG TGCTCTTTGA AAAGGCAGGA 
CTCCGACCCA GCCTGGACCC CTCCTCCCCA CCCGCATGTC CATTTGTCCA AGCCCTTTGG 
CTGCCTGAGC GGCCCCACAT TGGAGCTTGG TGCCTGGGGG GGCTCCACAT CAGGCTGGGG 
GTCATCAGGT GGAGAGACCA ATGTCTGGGG TCCCACAGGC CCACATCACA GCCCCGCCCC 
CTCCCAGAGC CCCACATGCA GGGTTGCAAA CCGCTGTGCT CCTGCCCCCA GAAAGTGTCC 
CACCACATCC TCCTGAAGGG CGCGGCCCGC GAGCACATGA TGTTCGTGGA GGGCGGAGAC 
CCCCTGGACG TGCCCGTGGA GTCCCTGACC GTGATCCCTG CCTTTGACCT GGAGCACAGA 
GAGGCTGAGG AGCTAAAGCC CATCCTGGTG ACCCTGGACT ACGCCCAGCT GGACCCAGAC 
ACACAGGCCC CACCTGCCAC CCGAGAACTG CAGGTGGGCT GCATCCGGAC TACCCAGCTG 
TCCGCAAAGA AGACTGTTGA GTTCAGCATG GACAACATCG CAGCCCTGCA GCACAAGGGC 
TTCACCATTG AGCCCTCCAG AGGCTCTGTG GAGCGCGGCC AGACCAAGGC CATCAGCATC 
TCCTGGACAC CACCCATCGA CTTTGATCCG GACCACCCAC TCTTGGCATC GGCCCTGCTG 
CAGCTCAGGG GGGATGTGAA GGAGACCTAC AAGGTCCTCT TTGTAGCCCA GGTGGTGACC 
GGTCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CFAP74 ( XM_024976793.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024832561.1 Bos taurus cilia and flagella associated protein 74 (CFAP74), transcript variant X4, mRNA.

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CloneID	OBa56330
Clone ID Related Accession (Same CDS sequence)	XM_024976793.1
Accession Version	XM_024976793.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4935bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	cilia- and flagella-associated protein 74 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAAG ACGACAAGAA GCTTTTGGAT GACAAGCCGC TCGTGGAAGA TGACACAGAG 
GAGCTGGAGG ACCCAGAGCT TGTGCTGGAC TGTGTCCCCA GGGAGGCTGA GCAGAAGGCC 
GGCCTGGGAA GCTCCTGTCC AGGAATAGAG CAGAGCAAGG ACAAGCAGAA GCAGAGGTTG 
ACTGCTCAGG AGAAAAGGCA GGCCTGCCTG CTGCGCAGGG ACTTGAGCCA CCTGGACCAG 
CTGCATGAAG AGAAAGACCT TTTCATCCAG AAGACAAGGG GGGAGCTACG TGCCTGCCGC 
AGGAGGATGG AACTCCTAGT CAAGCAGCAG GAGAGTGTGG CAGCCGAGAT AGCTGCAGAG 
AGGGCAGCCA ACAACATGGC AGCCATGGGC CGCCTGGAGG CAGCGTCGAG GCGCCTGTGC 
TCAGAGCTGA AGAACGAGCA GGACCTGCAG GCGCAGATGA TGGCGGTGCT GCAGCAGAGC 
CAGAGCGCCC TGTGGCAGAT TGAGGTCCAG GAGGGGCAGC TAGAGGACGC CCGCAAGTCG 
GCCCAAGAGG AGGCTGCAGC CGCCAGGAGA GGCCTTCAGG AGCGGGCAGC CAAGCAGCTG 
CACCGGGAGA TGGGGGCCTC GGGGAAGGCA GAGCGGAACA GGCTGCTGCG AGCCCGGAGA 
TGCGTGCACC TGCAGAAGGA GCTTGGACTC CGGCAGCAGA AGCTGGTGGA GGAAGCCCAG 
AAGAACCACC GGAAGGCCCT GAGGTTCCTG AAGGCCTCGT TGGGGAGGAT CCGTGAGCAG 
GAGCAGAAGG AGGAGCTGGA GACCCGAGAG CACATGCGCA GGCGCATGGA CGCGGTGCTG 
GCCCTGAAGA ACAGTATCTC CGCCAACCGG GAAACGCTCC GGAAATTTCA AGCATGGGGC 
CAGGCCAAGA CAGAGCTGGC AGAACAGAAG GCCCAGGCGG AGAAGAAGGC AATTCTGGCC 
CAAGGTGGGG ACGCCTTTAA GCACCTGTGC CATCAGCGCC GGCGCCAGGA GTTGGAGGCC 
CAGAACCGCG CCTTCGAGGA GGAGCAGAAG CGCAGGAAGC AGGAGATTGT GAGTCGGCTC 
TTGAAGGAAG AGGCTGTGGA GGACCGGAGG AGGCCACGGC CCGAGCCCCC GAGACCCGCA 
GGCCAGCGGA GCCTGAGGGA CCAGACCTGG AGCTACGTGG CCACCTTCTT CGATGGGCAG 
AGCACAGAGG CCCCGCTCTG CTGTCCCCTG CCTGATGTCC CTGGACAGAG GCCAGGTCCA 
GCCCAAGAGC AGATAAAGCT CCAGGAGACC AGGTCCAGAT TCTACCAGTC CTCTCCGCTG 
CTGACGGCCA TCTCCAGTGA GTCTGTCCAG GGGGACGGGA GCCTTGGCGG GGAGGACACA 
CTGGCTTGGC CGGAGATCCC CGGGCTCTGG AAGGAGGACT ACAAGCCTTT CCAGGTATCC 
AAGGAGGATG TGGACCGGAA GCCAGTGGGC GGAACAAAGA TGGACAAGGA CATCCTGGCG 
CGGACAATGC AACGGCTACG CAGTGGGGTG ATCCACAAAC AGGTGGCATC AGGGCGTGAG 
TTCAGAGGGT GCCCCTTCAA CAGCAAGCCC AAGTTCATCC ACTTCAAGGA CTTTGATGTG 
GGCAAAGTGT ACAAGAAGAA GATCACGCTG ATCAACGCCA CCTACACGAT TAACTACTGC 
AAGCTGGTGG GCGTGGATGA GAGCCTGCAG GACTTCATCC ACGTGGACTT TGACCCCCCA 
GGCCCACTAT CAGCCGGCAT GTCCTGCGAA GTGCTTGTCA CCTTCAAGCC CATGATAAAC 
AAAGATCTGG AAGGAAATGT CTCGTTTCTG GCTCAGACAG GCGGATTTTC TGTTCCACTG 
AAATGTTCAA CAAAGAAATG CTCTCTGTCC CTCGACAAGG AGCTCATCGA CTTTGGCAGC 
TACACAGTGG GTGAGACTGC AATCCGGACC ATCACGCTGA CCAACACTGG GGGCTTGGGC 
ACCAAGTTCA GATTTCTTCT GGCATCAGAG ACTTGTGAGA TGGACACCTC CCAGTCGGTC 
CTAAAATTAA GTAGTACCTT CACGTATGAA GACAAAAGTT TGTATGATAA ATTCACCACC 
AGTTTTTCTG AGCATCAGAT GGAGGGCAAT GAGTCATCTC CTACAGATGT ACCAAGCCAG 
AAGGAGTCTG GAAAGCTGGA CGTGAAGGAG CAGGAGGAGG GGCACCCCAT CGAATCAGAG 
GGCGTGACCT TGACCACCAT CATGACTATA CCTCTCAATG AGGAACAGAT GGAGTTCTCG 
CTGGGAGAGA CAACAGAGGG GGACATAGGC CCCTTCAGCT CCATCAAGGT GCCCGTCATC 
TTCACCCCGA TTGTCCCGGG AACGGTCCAG AACAGTTTCA AGGTTGTGTT TAAGAATCAG 
CAGTGCCCAA CTTTATATTT CAAAGTCGTC GGCGTGGCTA TTGATGTGCC TGTCTGGGTG 
CCAAAGCCCA ACGTAGACCT GAAGATCTGC ATGTACGATC GGCTGTACCA GGACTCGATC 
CTCGTCCACA CGCGGTCGAA GGCCGCGTTG CGCCTAAAGT TCGAGGTGTG CAAGGAGCTG 
CGGGGCCACG TGGAGCTCCT GCCTGAGACT GGCTACATCC AGGCCCTGTC GTCCTACTCA 
GTGCAGCTCA AGTTCCTGCC CAGACACTCC CTCCCAGAAG ATGCAGGGAA GTATTTTGAC 
AAGGAGACCA GGGTTCTGGA GGCCCCGATG ACTATCCGGG TGGCTGACCA GATCAAACCC 
GTGGGGTTTA CTGTCCATGC TGTAGTCACC ACCTCAGACC TGGAGCTCAG CCCCGCAGGG 
CTGGACTTTG GCTACTGTAC CATCTACGAG GCCATCAGGA CCCAAATCAG CCTCTGCAAT 
CATTCCCTCC TGCCCCAGGA GTTTGGGTTC GTTGGACTCC CCAAGTTTGT GGACATCCAG 
CCCAACGATG GGTTCGGGAC AATCCTGCCA CTGGAAACGC TACAGCTCGA TGTGATCTTT 
CAGCCCACCA AGGCCAAGGA GTACAACTTT GAGCTCATCT GCAAGTCAGA GATTAATCGG 
TGCTTCAAGC TGGCATGCCG GGCCGTGGGT GTCTACCCAC CCCTGGAGCT GTCCCACAAC 
CAGATCAAGT TTGCAGCCAC CTCCTTGTAC GACACATCCG TGGCCACTCT GTACGTCATC 
AACTCCCACC TGAGCATGAA CTCACTGACC CACTCAGTGC CCCGCATTGG CTCAGAGGAC 
GCTTTTCCCG TGGGGCCCAC GTCTTTTGAG TTCCTGCTGC CCCCAGATTC GCCCATCACC 
ATCTCACCCC TGGTGGGCAC CGTGTGGCCA GGAAAGAGGT GCCTGGTCCG AGTGGCCTTC 
CGGCCAGTGC TGCCCAGCGA GCTGATCCAC CAGGAGGCCT TCCAGGCCCT GAGCAGAAAA 
GCTGAGGTCA AATCGTCCCA AAAGGAGACG GTCACCCAGA GGAAGGACCA GCGGAGACCA 
TCTTTCTCCA TGCTGCGGCT GCAGAACCAA GACCGGCTGC TCCGGGCCTC AGCCTCCCAG 
CCCCCGGAGC TGCAGAAGGA GGACCTTGCA TCCAGCTCTC AAGAGTACCA GTCGGCACAG 
GCCTCCCTGA CCAGGTCTTT CCAGGGGAAG TTTGACAAGT TTGTGGTTCC CTGTGTTGTT 
GCTAGCGGCG ACACCAAAGA CAGGAAGGGG ACAGAACCGC TGAGCTTCAG TCCCCACAAC 
ACGCTGTACC TGGAGCTGTG GTGTCCAGCA GTGGCGCCGT CTATCGTGGT GACGTCCGAC 
AAAGGCAAGA CCATCATTAA CTTCGGCGAC ATTGCTGCAG GACACCGGGG CATAAAGAAG 
GTCTCCATCC AGAACATCTC CCCTGAGGAT CTCGACCTGG AGTACTCCGT GCTGAACCCC 
GATGGCCCCT TCGTCCTGCT GAACCCCACC ATCAGGTTGC CCTCTGGCGA GACCCAGGTC 
CTAGATCTGT CCTTCTCCCC ACGTGAGAGT GTCGTGGCCC AAGAAATACT GGACATCATC 
ACCAAGAAAG GCACGCTCTC CCTGGCCCTC ATGGGCACGG GTGTGGCGTC CACGATCACG 
TGCTCCATTG AAGGTGATGT CCTCAACATG GGCTACGTGA TCGCCAGAGA GTCTGTCTCC 
TTGGGCTTCA AGCTGCAGAA CAACTCCCCG CTGCCCATCA AGTTCTCCAT GCAGCTGGAC 
AGCCTCTCCT CCAGGAGCAG AGACCAGCAC CGCCTGCCAC AGTTCCTGGC CTCGCCTGAC 
CAGAGGACGG AAGTCGTGGG CACGCAGAAC TACAGTGGCC AGAGCGTGTT CAGCGTGGTC 
CCGGTCGAGG GCGTCATGGA GCCCGGCAAG GCTCAGGACT TCACTGTGAC CTTCAGCCCA 
GACCACGAGA GCCTATACTT CTCTGACCGG CTGCAGGTCG TGCTCTTTGA AAAGAAAGTG 
TCCCACCACA TCCTCCTGAA GGGCGCGGCC CGCGAGCACA TGATGTTCGT GGAGGGCGGA 
GACCCCCTGG ACGTGCCCGT GGAGTCCCTG ACCGTGATCC CTGCCTTTGA CCTGGAGCAC 
AGAGAGGAAG CTGAGGAGCT AAAGCCCATC CTGGTGACCC TGGACTACGC CCAGCTGGAC 
CCAGACACAC AGGCCCCACC TGCCACCCGA GAACTGCAGG TGGGCTGCAT CCGGACTACC 
CAGCTGTCCG CAAAGAAGAC TGTTGAGTTC AGCATGGACA ACATCGCAGC CCTGCAGCAC 
AAGGGCTTCA CCATTGAGCC CTCCAGAGGC TCTGTGGAGC GCGGCCAGAC CAAGGCCATC 
AGCATCTCCT GGACACCACC CATCGACTTT GATCCGGACC ACCCACTCTT GGCATCGGCC 
CTGCTGCAGC TCAGGGGGGA TGTGAAGGAG ACCTACAAGG TCCTCTTTGT AGCCCAGGTG 
GTGACCGGTC CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024832561.1
CDS	415..5349
Translation	MEEDDKKLLDDKPLVEDDTEELEDPELVLDCVPREAEQKAGLGSSCPGIEQSKDKQKQRLTAQEKRQACLLRRDLSHLDQLHEEKDLFIQKTRGELRACRRRMELLVKQQESVAAEIAAERAANNMAAMGRLEAASRRLCSELKNEQDLQAQMMAVLQQSQSALWQIEVQEGQLEDARKSAQEEAAAARRGLQERAAKQLHREMGASGKAERNRLLRARRCVHLQKELGLRQQKLVEEAQKNHRKALRFLKASLGRIREQEQKEELETREHMRRRMDAVLALKNSISANRETLRKFQAWGQAKTELAEQKAQAEKKAILAQGGDAFKHLCHQRRRQELEAQNRAFEEEQKRRKQEIVSRLLKEEAVEDRRRPRPEPPRPAGQRSLRDQTWSYVATFFDGQSTEAPLCCPLPDVPGQRPGPAQEQIKLQETRSRFYQSSPLLTAISSESVQGDGSLGGEDTLAWPEIPGLWKEDYKPFQVSKEDVDRKPVGGTKMDKDILARTMQRLRSGVIHKQVASGREFRGCPFNSKPKFIHFKDFDVGKVYKKKITLINATYTINYCKLVGVDESLQDFIHVDFDPPGPLSAGMSCEVLVTFKPMINKDLEGNVSFLAQTGGFSVPLKCSTKKCSLSLDKELIDFGSYTVGETAIRTITLTNTGGLGTKFRFLLASETCEMDTSQSVLKLSSTFTYEDKSLYDKFTTSFSEHQMEGNESSPTDVPSQKESGKLDVKEQEEGHPIESEGVTLTTIMTIPLNEEQMEFSLGETTEGDIGPFSSIKVPVIFTPIVPGTVQNSFKVVFKNQQCPTLYFKVVGVAIDVPVWVPKPNVDLKICMYDRLYQDSILVHTRSKAALRLKFEVCKELRGHVELLPETGYIQALSSYSVQLKFLPRHSLPEDAGKYFDKETRVLEAPMTIRVADQIKPVGFTVHAVVTTSDLELSPAGLDFGYCTIYEAIRTQISLCNHSLLPQEFGFVGLPKFVDIQPNDGFGTILPLETLQLDVIFQPTKAKEYNFELICKSEINRCFKLACRAVGVYPPLELSHNQIKFAATSLYDTSVATLYVINSHLSMNSLTHSVPRIGSEDAFPVGPTSFEFLLPPDSPITISPLVGTVWPGKRCLVRVAFRPVLPSELIHQEAFQALSRKAEVKSSQKETVTQRKDQRRPSFSMLRLQNQDRLLRASASQPPELQKEDLASSSQEYQSAQASLTRSFQGKFDKFVVPCVVASGDTKDRKGTEPLSFSPHNTLYLELWCPAVAPSIVVTSDKGKTIINFGDIAAGHRGIKKVSIQNISPEDLDLEYSVLNPDGPFVLLNPTIRLPSGETQVLDLSFSPRESVVAQEILDIITKKGTLSLALMGTGVASTITCSIEGDVLNMGYVIARESVSLGFKLQNNSPLPIKFSMQLDSLSSRSRDQHRLPQFLASPDQRTEVVGTQNYSGQSVFSVVPVEGVMEPGKAQDFTVTFSPDHESLYFSDRLQVVLFEKKVSHHILLKGAAREHMMFVEGGDPLDVPVESLTVIPAFDLEHREEAEELKPILVTLDYAQLDPDTQAPPATRELQVGCIRTTQLSAKKTVEFSMDNIAALQHKGFTIEPSRGSVERGQTKAISISWTPPIDFDPDHPLLASALLQLRGDVKETYKVLFVAQVVTGP

Target ORF information:

RefSeq Version	XM_024976793.1
Organism	Bos taurus(cattle)
Definition	Bos taurus cilia and flagella associated protein 74 (CFAP74), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024976793.1

ORF Insert Sequence:

ATGGAAGAAG ACGACAAGAA GCTTTTGGAT GACAAGCCGC TCGTGGAAGA TGACACAGAG 
GAGCTGGAGG ACCCAGAGCT TGTGCTGGAC TGTGTCCCCA GGGAGGCTGA GCAGAAGGCC 
GGCCTGGGAA GCTCCTGTCC AGGAATAGAG CAGAGCAAGG ACAAGCAGAA GCAGAGGTTG 
ACTGCTCAGG AGAAAAGGCA GGCCTGCCTG CTGCGCAGGG ACTTGAGCCA CCTGGACCAG 
CTGCATGAAG AGAAAGACCT TTTCATCCAG AAGACAAGGG GGGAGCTACG TGCCTGCCGC 
AGGAGGATGG AACTCCTAGT CAAGCAGCAG GAGAGTGTGG CAGCCGAGAT AGCTGCAGAG 
AGGGCAGCCA ACAACATGGC AGCCATGGGC CGCCTGGAGG CAGCGTCGAG GCGCCTGTGC 
TCAGAGCTGA AGAACGAGCA GGACCTGCAG GCGCAGATGA TGGCGGTGCT GCAGCAGAGC 
CAGAGCGCCC TGTGGCAGAT TGAGGTCCAG GAGGGGCAGC TAGAGGACGC CCGCAAGTCG 
GCCCAAGAGG AGGCTGCAGC CGCCAGGAGA GGCCTTCAGG AGCGGGCAGC CAAGCAGCTG 
CACCGGGAGA TGGGGGCCTC GGGGAAGGCA GAGCGGAACA GGCTGCTGCG AGCCCGGAGA 
TGCGTGCACC TGCAGAAGGA GCTTGGACTC CGGCAGCAGA AGCTGGTGGA GGAAGCCCAG 
AAGAACCACC GGAAGGCCCT GAGGTTCCTG AAGGCCTCGT TGGGGAGGAT CCGTGAGCAG 
GAGCAGAAGG AGGAGCTGGA GACCCGAGAG CACATGCGCA GGCGCATGGA CGCGGTGCTG 
GCCCTGAAGA ACAGTATCTC CGCCAACCGG GAAACGCTCC GGAAATTTCA AGCATGGGGC 
CAGGCCAAGA CAGAGCTGGC AGAACAGAAG GCCCAGGCGG AGAAGAAGGC AATTCTGGCC 
CAAGGTGGGG ACGCCTTTAA GCACCTGTGC CATCAGCGCC GGCGCCAGGA GTTGGAGGCC 
CAGAACCGCG CCTTCGAGGA GGAGCAGAAG CGCAGGAAGC AGGAGATTGT GAGTCGGCTC 
TTGAAGGAAG AGGCTGTGGA GGACCGGAGG AGGCCACGGC CCGAGCCCCC GAGACCCGCA 
GGCCAGCGGA GCCTGAGGGA CCAGACCTGG AGCTACGTGG CCACCTTCTT CGATGGGCAG 
AGCACAGAGG CCCCGCTCTG CTGTCCCCTG CCTGATGTCC CTGGACAGAG GCCAGGTCCA 
GCCCAAGAGC AGATAAAGCT CCAGGAGACC AGGTCCAGAT TCTACCAGTC CTCTCCGCTG 
CTGACGGCCA TCTCCAGTGA GTCTGTCCAG GGGGACGGGA GCCTTGGCGG GGAGGACACA 
CTGGCTTGGC CGGAGATCCC CGGGCTCTGG AAGGAGGACT ACAAGCCTTT CCAGGTATCC 
AAGGAGGATG TGGACCGGAA GCCAGTGGGC GGAACAAAGA TGGACAAGGA CATCCTGGCG 
CGGACAATGC AACGGCTACG CAGTGGGGTG ATCCACAAAC AGGTGGCATC AGGGCGTGAG 
TTCAGAGGGT GCCCCTTCAA CAGCAAGCCC AAGTTCATCC ACTTCAAGGA CTTTGATGTG 
GGCAAAGTGT ACAAGAAGAA GATCACGCTG ATCAACGCCA CCTACACGAT TAACTACTGC 
AAGCTGGTGG GCGTGGATGA GAGCCTGCAG GACTTCATCC ACGTGGACTT TGACCCCCCA 
GGCCCACTAT CAGCCGGCAT GTCCTGCGAA GTGCTTGTCA CCTTCAAGCC CATGATAAAC 
AAAGATCTGG AAGGAAATGT CTCGTTTCTG GCTCAGACAG GCGGATTTTC TGTTCCACTG 
AAATGTTCAA CAAAGAAATG CTCTCTGTCC CTCGACAAGG AGCTCATCGA CTTTGGCAGC 
TACACAGTGG GTGAGACTGC AATCCGGACC ATCACGCTGA CCAACACTGG GGGCTTGGGC 
ACCAAGTTCA GATTTCTTCT GGCATCAGAG ACTTGTGAGA TGGACACCTC CCAGTCGGTC 
CTAAAATTAA GTAGTACCTT CACGTATGAA GACAAAAGTT TGTATGATAA ATTCACCACC 
AGTTTTTCTG AGCATCAGAT GGAGGGCAAT GAGTCATCTC CTACAGATGT ACCAAGCCAG 
AAGGAGTCTG GAAAGCTGGA CGTGAAGGAG CAGGAGGAGG GGCACCCCAT CGAATCAGAG 
GGCGTGACCT TGACCACCAT CATGACTATA CCTCTCAATG AGGAACAGAT GGAGTTCTCG 
CTGGGAGAGA CAACAGAGGG GGACATAGGC CCCTTCAGCT CCATCAAGGT GCCCGTCATC 
TTCACCCCGA TTGTCCCGGG AACGGTCCAG AACAGTTTCA AGGTTGTGTT TAAGAATCAG 
CAGTGCCCAA CTTTATATTT CAAAGTCGTC GGCGTGGCTA TTGATGTGCC TGTCTGGGTG 
CCAAAGCCCA ACGTAGACCT GAAGATCTGC ATGTACGATC GGCTGTACCA GGACTCGATC 
CTCGTCCACA CGCGGTCGAA GGCCGCGTTG CGCCTAAAGT TCGAGGTGTG CAAGGAGCTG 
CGGGGCCACG TGGAGCTCCT GCCTGAGACT GGCTACATCC AGGCCCTGTC GTCCTACTCA 
GTGCAGCTCA AGTTCCTGCC CAGACACTCC CTCCCAGAAG ATGCAGGGAA GTATTTTGAC 
AAGGAGACCA GGGTTCTGGA GGCCCCGATG ACTATCCGGG TGGCTGACCA GATCAAACCC 
GTGGGGTTTA CTGTCCATGC TGTAGTCACC ACCTCAGACC TGGAGCTCAG CCCCGCAGGG 
CTGGACTTTG GCTACTGTAC CATCTACGAG GCCATCAGGA CCCAAATCAG CCTCTGCAAT 
CATTCCCTCC TGCCCCAGGA GTTTGGGTTC GTTGGACTCC CCAAGTTTGT GGACATCCAG 
CCCAACGATG GGTTCGGGAC AATCCTGCCA CTGGAAACGC TACAGCTCGA TGTGATCTTT 
CAGCCCACCA AGGCCAAGGA GTACAACTTT GAGCTCATCT GCAAGTCAGA GATTAATCGG 
TGCTTCAAGC TGGCATGCCG GGCCGTGGGT GTCTACCCAC CCCTGGAGCT GTCCCACAAC 
CAGATCAAGT TTGCAGCCAC CTCCTTGTAC GACACATCCG TGGCCACTCT GTACGTCATC 
AACTCCCACC TGAGCATGAA CTCACTGACC CACTCAGTGC CCCGCATTGG CTCAGAGGAC 
GCTTTTCCCG TGGGGCCCAC GTCTTTTGAG TTCCTGCTGC CCCCAGATTC GCCCATCACC 
ATCTCACCCC TGGTGGGCAC CGTGTGGCCA GGAAAGAGGT GCCTGGTCCG AGTGGCCTTC 
CGGCCAGTGC TGCCCAGCGA GCTGATCCAC CAGGAGGCCT TCCAGGCCCT GAGCAGAAAA 
GCTGAGGTCA AATCGTCCCA AAAGGAGACG GTCACCCAGA GGAAGGACCA GCGGAGACCA 
TCTTTCTCCA TGCTGCGGCT GCAGAACCAA GACCGGCTGC TCCGGGCCTC AGCCTCCCAG 
CCCCCGGAGC TGCAGAAGGA GGACCTTGCA TCCAGCTCTC AAGAGTACCA GTCGGCACAG 
GCCTCCCTGA CCAGGTCTTT CCAGGGGAAG TTTGACAAGT TTGTGGTTCC CTGTGTTGTT 
GCTAGCGGCG ACACCAAAGA CAGGAAGGGG ACAGAACCGC TGAGCTTCAG TCCCCACAAC 
ACGCTGTACC TGGAGCTGTG GTGTCCAGCA GTGGCGCCGT CTATCGTGGT GACGTCCGAC 
AAAGGCAAGA CCATCATTAA CTTCGGCGAC ATTGCTGCAG GACACCGGGG CATAAAGAAG 
GTCTCCATCC AGAACATCTC CCCTGAGGAT CTCGACCTGG AGTACTCCGT GCTGAACCCC 
GATGGCCCCT TCGTCCTGCT GAACCCCACC ATCAGGTTGC CCTCTGGCGA GACCCAGGTC 
CTAGATCTGT CCTTCTCCCC ACGTGAGAGT GTCGTGGCCC AAGAAATACT GGACATCATC 
ACCAAGAAAG GCACGCTCTC CCTGGCCCTC ATGGGCACGG GTGTGGCGTC CACGATCACG 
TGCTCCATTG AAGGTGATGT CCTCAACATG GGCTACGTGA TCGCCAGAGA GTCTGTCTCC 
TTGGGCTTCA AGCTGCAGAA CAACTCCCCG CTGCCCATCA AGTTCTCCAT GCAGCTGGAC 
AGCCTCTCCT CCAGGAGCAG AGACCAGCAC CGCCTGCCAC AGTTCCTGGC CTCGCCTGAC 
CAGAGGACGG AAGTCGTGGG CACGCAGAAC TACAGTGGCC AGAGCGTGTT CAGCGTGGTC 
CCGGTCGAGG GCGTCATGGA GCCCGGCAAG GCTCAGGACT TCACTGTGAC CTTCAGCCCA 
GACCACGAGA GCCTATACTT CTCTGACCGG CTGCAGGTCG TGCTCTTTGA AAAGAAAGTG 
TCCCACCACA TCCTCCTGAA GGGCGCGGCC CGCGAGCACA TGATGTTCGT GGAGGGCGGA 
GACCCCCTGG ACGTGCCCGT GGAGTCCCTG ACCGTGATCC CTGCCTTTGA CCTGGAGCAC 
AGAGAGGAAG CTGAGGAGCT AAAGCCCATC CTGGTGACCC TGGACTACGC CCAGCTGGAC 
CCAGACACAC AGGCCCCACC TGCCACCCGA GAACTGCAGG TGGGCTGCAT CCGGACTACC 
CAGCTGTCCG CAAAGAAGAC TGTTGAGTTC AGCATGGACA ACATCGCAGC CCTGCAGCAC 
AAGGGCTTCA CCATTGAGCC CTCCAGAGGC TCTGTGGAGC GCGGCCAGAC CAAGGCCATC 
AGCATCTCCT GGACACCACC CATCGACTTT GATCCGGACC ACCCACTCTT GGCATCGGCC 
CTGCTGCAGC TCAGGGGGGA TGTGAAGGAG ACCTACAAGG TCCTCTTTGT AGCCCAGGTG 
GTGACCGGTC CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CFAP74 ( XM_024976794.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024832562.1 Bos taurus cilia and flagella associated protein 74 (CFAP74), transcript variant X5, mRNA.

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CloneID	OBa56331
Clone ID Related Accession (Same CDS sequence)	XM_024976794.1
Accession Version	XM_024976794.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4920bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	cilia- and flagella-associated protein 74 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAAG ACGACAAGAA GCTTTTGGAT GACAAGCCGC TCGTGGAAGA TGACACAGAG 
GAGCTGGAGG ACCCAGAGCT TGTGCTGGAC TGTGTCCCCA GGGAGGCTGA GCAGAAGGCC 
GGCCTGGGAA GCTCCTGTCC AGGAATAGAG CAGAGCAAGG ACAAGCAGAA GCAGAGGTTG 
ACTGCTCAGG AGAAAAGGCA GGCCTGCCTG CTGCGCAGGG ACTTGAGCCA CCTGGACCAG 
CTGCATGAAG AGAAAGACCT TTTCATCCAG AAGACAAGGG GGGAGCTACG TGCCTGCCGC 
AGGAGGATGG AACTCCTAGT CAAGCAGCAG GAGAGTGTGG CAGCCGAGAT AGCTGCAGAG 
AGGGCAGCCA ACAACATGGC AGCCATGGGC CGCCTGGAGG CAGCGTCGAG GCGCCTGTGC 
TCAGAGCTGA AGAACGAGCA GGACCTGCAG GCGCAGATGA TGGCGGTGCT GCAGCAGAGC 
CAGAGCGCCC TGTGGCAGAT TGAGGTCCAG GAGGGGCAGC TAGAGGACGC CCGCAAGTCG 
GCCCAAGAGG AGGCTGCAGC CGCCAGGAGA GGCCTTCAGG AGCGGGCAGC CAAGCAGCTG 
CACCGGGAGA TGGGGGCCTC GGGGAAGGCA GAGCGGAACA GGCTGCTGCG AGCCCGGAGA 
TGCGTGCACC TGCAGAAGGA GCTTGGACTC CGGCAGCAGA AGCTGGTGGA GGAAGCCCAG 
AAGAACCACC GGAAGGCCCT GAGGTTCCTG AAGGCCTCGT TGGGGAGGAT CCGTGAGCAG 
GAGCAGAAGG AGGAGCTGGA GACCCGAGAG CACATGCGCA GGCGCATGGA CGCGGTGCTG 
GCCCTGAAGA ACAGTATCTC CGCCAACCGG GAAACGCTCC GGAAATTTCA AGCATGGGGC 
CAGGCCAAGA CAGAGCTGGC AGAACAGAAG GCCCAGGCGG AGAAGAAGGC AATTCTGGCC 
CAAGGTGGGG ACGCCTTTAA GCACCTGTGC CATCAGCGCC GGCGCCAGGA GTTGGAGGCC 
CAGAACCGCG CCTTCGAGGA GGAGCAGAAG CGCAGGAAGC AGGAGATTGT GAGTCGGCTC 
TTGAAGGAAG AGGCTGTGGA GGACCGGAGG AGGCCACGGC CCGAGCCCCC GAGACCCGCA 
GGCCAGCGGA GCCTGAGGGA CCAGACCTGG AGCTACGTGG CCACCTTCTT CGATGGGCAG 
AGCACAGAGG CCCCGCTCTG CTGTCCCCTG CCTGATGTCC CTGGACAGAG GCCAGGTCCA 
GCCCAAGAGC AGATAAAGCT CCAGGAGACC AGGTCCAGAT TCTACCAGTC CTCTCCGCTG 
CTGACGGCCA TCTCCAGTGA GTCTGTCCAG GGGGACGGGA GCCTTGGCGG GGAGGACACA 
CTGGCTTGGC CGGAGATCCC CGGGCTCTGG AAGGAGGACT ACAAGCCTTT CCAGGTATCC 
AAGGAGGATG TGGACCGGAA GCCAGTGGGC GGAACAAAGA TGGACAAGGA CATCCTGGCG 
CGGACAATGC AACGGCTACG CAGTGGGGTG ATCCACAAAC AGGTGGCATC AGGGCGTGAG 
TTCAGAGGGT GCCCCTTCAA CAGCAAGCCC AAGTTCATCC ACTTCAAGGA CTTTGATGTG 
GGCAAAGTGT ACAAGAAGAA GATCACGCTG ATCAACGCCA CCTACACGAT TAACTACTGC 
AAGCTGGTGG GCGTGGATGA GAGCCTGCAG GACTTCATCC ACGTGGACTT TGACCCCCCA 
GGCCCACTAT CAGCCGGCAT GTCCTGCGAA GTGCTTGTCA CCTTCAAGCC CATGATAAAC 
AAAGATCTGG AAGGAAATGT CTCGTTTCTG GCTCAGACAG GCGGATTTTC TGTTCCACTG 
AAATGTTCAA CAAAGAAATG CTCTCTGTCC CTCGACAAGG AGCTCATCGA CTTTGGCAGC 
TACACAGTGG GTGAGACTGC AATCCGGACC ATCACGCTGA CCAACACTGG GGGCTTGGGC 
ACCAAGTTCA GATTTCTTCT GGCATCAGAG ACTTGTGAGA TGGACACCTC CCAGTCGGTC 
CTAAAATTAA GTAGTACCTT CACGTATGAA GACAAAAGTT TGTATGATAA ATTCACCACC 
AGTTTTTCTG AGCATCAGAT GGAGGGCAAT GAGTCATCTC CTACAGATGT ACCAAGCCAG 
AAGGAGTCTG GAAAGCTGGA CGTGAAGGAG CAGGAGGAGG GGCACCCCAT CGAATCAGAG 
GGCGTGACCT TGACCACCAT CATGACTATA CCTCTCAATG AGGAACAGAT GGAGTTCTCG 
CTGGGAGAGA CAACAGAGGG GGACATAGGC CCCTTCAGCT CCATCAAGGT GCCCGTCATC 
TTCACCCCGA TTGTCCCGGG AACGGTCCAG AACAGTTTCA AGGTTGTGTT TAAGAATCAG 
CAGTGCCCAA CTTTATATTT CAAAGTCGTC GGCGTGGCTA TTGATGTGCC TGTCTGGGTG 
CCAAAGCCCA ACGTAGACCT GAAGATCTGC ATGTACGATC GGCTGTACCA GGACTCGATC 
CTCGTCCACA CGCGGTCGAA GGCCGCGTTG CGCCTAAAGT TCGAGGTGTG CAAGGAGCTG 
CGGGGCCACG TGGAGCTCCT GCCTGAGACT GGCTACATCC AGGCCCTGTC GTCCTACTCA 
GTGCAGCTCA AGTTCCTGCC CAGACACTCC CTCCCAGAAG ATGCAGGGAA GTATTTTGAC 
AAGGAGACCA GGGTTCTGGA GGCCCCGATG ACTATCCGGG TGGCTGACCA GATCAAACCC 
GTGGGGTTTA CTGTCCATGC TGTAGTCACC ACCTCAGACC TGGAGCTCAG CCCCGCAGGG 
CTGGACTTTG GCTACTGTAC CATCTACGAG GCCATCAGGA CCCAAATCAG CCTCTGCAAT 
CATTCCCTCC TGCCCCAGGA GTTTGGGTTC GTTGGACTCC CCAAGTTTGT GGACATCCAG 
CCCAACGATG GGTTCGGGAC AATCCTGCCA CTGGAAACGC TACAGCTCGA TGTGATCTTT 
CAGCCCACCA AGGCCAAGGA GTACAACTTT GAGCTCATCT GCAAGTCAGA GATTAATCGG 
TGCTTCAAGC TGGCATGCCG GGCCGTGGGT GTCTACCCAC CCCTGGAGCT GTCCCACAAC 
CAGATCAAGT TTGCAGCCAC CTCCTTGTAC GACACATCCG TGGCCACTCT GTACGTCATC 
AACTCCCACC TGAGCATGAA CTCACTGACC CACTCAGTGC CCCGCATTGG CTCAGAGGAC 
GCTTTTCCCG TGGGGCCCAC GTCTTTTGAG TTCCTGCTGC CCCCAGATTC GCCCATCACC 
ATCTCACCCC TGGTGGGCAC CGTGTGGCCA GGAAAGAGGT GCCTGGTCCG AGTGGCCTTC 
CGGCCAGTGC TGCCCAGCGA GCTGATCCAC CAGGAGGCCT TCCAGGCCCT GAGCAGAAAA 
GCTGAGGTCA AATCGTCCCA AAAGGAGACG GTCACCCAGA GGAAGGACCA GCGGAGACCA 
TCTTTCTCCA TGCTGCGGCT GCAGAACCAA GACCGGCTGC TCCGGGCCTC AGCCTCCCAG 
CCCCCGGAGC TGCAGAAGGA GGACCTTGCA TCCAGCTCTC AAGAGTACCA GTCGGCACAG 
GCCTCCCTGA CCAGGTCTTT CCAGGGGAAG TTTGACAAGT TTGTGGTTCC CTGTGTTGTT 
GCTAGCGGCG ACACCAAAGA CAGGAAGGGG ACAGAACCGC TGAGCTTCAG TCCCCACAAC 
ACGCTGTACC TGGAGCTGTG GTGTCCAGCA GTGGCGCCGT CTATCGTGGT GACGTCCGAC 
AAAGGCAAGA CCATCATTAA CTTCGGCGAC ATTGCTGCAG GACACCGGGG CATAAAGAAG 
GTCTCCATCC AGAACATCTC CCCTGAGGAT CTCGACCTGG AGTACTCCGT GCTGAACCCC 
GATGGCCCCT TCGTCCTGCT GAACCCCACC ATCAGGTTGC CCTCTGGCGA GACCCAGGTC 
CTAGATCTGT CCTTCTCCCC ACGTGAGAGT GTCGTGGCCC AAGAAATACT GGACATCATC 
ACCAAGAAAG GCACGCTCTC CCTGGCCCTC ATGGGCACGG GTGTGGCGTC CACGATCACG 
TGCTCCATTG AAGGTGATGT CCTCAACATG GGCTACGTGA TCGCCAGAGA GTCTGTCTCC 
TTGGGCTTCA AGCTGCAGAA CAACTCCCCG CTGCCCATCA AGTTCTCCAT GCAGCTGGAC 
AGCCTCTCCT CCAGGAGCAG AGACCAGCAC CGCCTGCCAC AGTTCCTGGC CTCGCCTGAC 
CAGAGGACGG AAGTCGTGGG CACGCAGAAC TACAGTGGCC AGAGCGTGTT CAGCGTGGTC 
CCGGTCGAGG GCGTCATGGA GCCCGGCAAG GCTCAGGACT TCACTGTGAC CTTCAGCCCA 
GACCACGAGA GCCTATACTT CTCTGACCGG CTGCAGGTCG TGCTCTTTGA AAAGGCAGGA 
CTCCGACCCA GCCTGGACCC CTCCTCCCCA CCCGCATGTC CATTTGTCCA AGCCCTTTGG 
CTGCCTGAGC GGCCCCACAT TGGAGCTTGG TGCCTGGGGG GGCTCCACAT CAGGCTGGGG 
GTCATCAGGT GGAGAGACCA ATGTCTGGGG TCCCACAGGC CCACATCACA GCCCCGCCCC 
CTCCCAGAGC CCCACATGCA GGGTTGCAAA CCGCTGTGCT CCTGCCCCCA GAAAGTGTCC 
CACCACATCC TCCTGAAGGG CGCGGCCCGC GAGCACATGA TGTTCGTGGA GGGCGGAGAC 
CCCCTGGACG TGCCCGTGGA GTCCCTGACC GTGATCCCTG CCTTTGACCT GGAGCACAGA 
GAGGGTGAGC CCTCTGAGGG CAGAGAGCCC CGAGGTGGGC AAGGTGGCAG AAGAGGACAC 
ATGACCTTTG GAGTCTGCAG GGAGGGCAGG CATTCCTGGC CCATCCACCA CAGAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024832562.1
CDS	417..5336
Translation	MEEDDKKLLDDKPLVEDDTEELEDPELVLDCVPREAEQKAGLGSSCPGIEQSKDKQKQRLTAQEKRQACLLRRDLSHLDQLHEEKDLFIQKTRGELRACRRRMELLVKQQESVAAEIAAERAANNMAAMGRLEAASRRLCSELKNEQDLQAQMMAVLQQSQSALWQIEVQEGQLEDARKSAQEEAAAARRGLQERAAKQLHREMGASGKAERNRLLRARRCVHLQKELGLRQQKLVEEAQKNHRKALRFLKASLGRIREQEQKEELETREHMRRRMDAVLALKNSISANRETLRKFQAWGQAKTELAEQKAQAEKKAILAQGGDAFKHLCHQRRRQELEAQNRAFEEEQKRRKQEIVSRLLKEEAVEDRRRPRPEPPRPAGQRSLRDQTWSYVATFFDGQSTEAPLCCPLPDVPGQRPGPAQEQIKLQETRSRFYQSSPLLTAISSESVQGDGSLGGEDTLAWPEIPGLWKEDYKPFQVSKEDVDRKPVGGTKMDKDILARTMQRLRSGVIHKQVASGREFRGCPFNSKPKFIHFKDFDVGKVYKKKITLINATYTINYCKLVGVDESLQDFIHVDFDPPGPLSAGMSCEVLVTFKPMINKDLEGNVSFLAQTGGFSVPLKCSTKKCSLSLDKELIDFGSYTVGETAIRTITLTNTGGLGTKFRFLLASETCEMDTSQSVLKLSSTFTYEDKSLYDKFTTSFSEHQMEGNESSPTDVPSQKESGKLDVKEQEEGHPIESEGVTLTTIMTIPLNEEQMEFSLGETTEGDIGPFSSIKVPVIFTPIVPGTVQNSFKVVFKNQQCPTLYFKVVGVAIDVPVWVPKPNVDLKICMYDRLYQDSILVHTRSKAALRLKFEVCKELRGHVELLPETGYIQALSSYSVQLKFLPRHSLPEDAGKYFDKETRVLEAPMTIRVADQIKPVGFTVHAVVTTSDLELSPAGLDFGYCTIYEAIRTQISLCNHSLLPQEFGFVGLPKFVDIQPNDGFGTILPLETLQLDVIFQPTKAKEYNFELICKSEINRCFKLACRAVGVYPPLELSHNQIKFAATSLYDTSVATLYVINSHLSMNSLTHSVPRIGSEDAFPVGPTSFEFLLPPDSPITISPLVGTVWPGKRCLVRVAFRPVLPSELIHQEAFQALSRKAEVKSSQKETVTQRKDQRRPSFSMLRLQNQDRLLRASASQPPELQKEDLASSSQEYQSAQASLTRSFQGKFDKFVVPCVVASGDTKDRKGTEPLSFSPHNTLYLELWCPAVAPSIVVTSDKGKTIINFGDIAAGHRGIKKVSIQNISPEDLDLEYSVLNPDGPFVLLNPTIRLPSGETQVLDLSFSPRESVVAQEILDIITKKGTLSLALMGTGVASTITCSIEGDVLNMGYVIARESVSLGFKLQNNSPLPIKFSMQLDSLSSRSRDQHRLPQFLASPDQRTEVVGTQNYSGQSVFSVVPVEGVMEPGKAQDFTVTFSPDHESLYFSDRLQVVLFEKAGLRPSLDPSSPPACPFVQALWLPERPHIGAWCLGGLHIRLGVIRWRDQCLGSHRPTSQPRPLPEPHMQGCKPLCSCPQKVSHHILLKGAAREHMMFVEGGDPLDVPVESLTVIPAFDLEHREGEPSEGREPRGGQGGRRGHMTFGVCREGRHSWPIHHRS

Target ORF information:

RefSeq Version	XM_024976794.1
Organism	Bos taurus(cattle)
Definition	Bos taurus cilia and flagella associated protein 74 (CFAP74), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024976794.1

ORF Insert Sequence:

ATGGAAGAAG ACGACAAGAA GCTTTTGGAT GACAAGCCGC TCGTGGAAGA TGACACAGAG 
GAGCTGGAGG ACCCAGAGCT TGTGCTGGAC TGTGTCCCCA GGGAGGCTGA GCAGAAGGCC 
GGCCTGGGAA GCTCCTGTCC AGGAATAGAG CAGAGCAAGG ACAAGCAGAA GCAGAGGTTG 
ACTGCTCAGG AGAAAAGGCA GGCCTGCCTG CTGCGCAGGG ACTTGAGCCA CCTGGACCAG 
CTGCATGAAG AGAAAGACCT TTTCATCCAG AAGACAAGGG GGGAGCTACG TGCCTGCCGC 
AGGAGGATGG AACTCCTAGT CAAGCAGCAG GAGAGTGTGG CAGCCGAGAT AGCTGCAGAG 
AGGGCAGCCA ACAACATGGC AGCCATGGGC CGCCTGGAGG CAGCGTCGAG GCGCCTGTGC 
TCAGAGCTGA AGAACGAGCA GGACCTGCAG GCGCAGATGA TGGCGGTGCT GCAGCAGAGC 
CAGAGCGCCC TGTGGCAGAT TGAGGTCCAG GAGGGGCAGC TAGAGGACGC CCGCAAGTCG 
GCCCAAGAGG AGGCTGCAGC CGCCAGGAGA GGCCTTCAGG AGCGGGCAGC CAAGCAGCTG 
CACCGGGAGA TGGGGGCCTC GGGGAAGGCA GAGCGGAACA GGCTGCTGCG AGCCCGGAGA 
TGCGTGCACC TGCAGAAGGA GCTTGGACTC CGGCAGCAGA AGCTGGTGGA GGAAGCCCAG 
AAGAACCACC GGAAGGCCCT GAGGTTCCTG AAGGCCTCGT TGGGGAGGAT CCGTGAGCAG 
GAGCAGAAGG AGGAGCTGGA GACCCGAGAG CACATGCGCA GGCGCATGGA CGCGGTGCTG 
GCCCTGAAGA ACAGTATCTC CGCCAACCGG GAAACGCTCC GGAAATTTCA AGCATGGGGC 
CAGGCCAAGA CAGAGCTGGC AGAACAGAAG GCCCAGGCGG AGAAGAAGGC AATTCTGGCC 
CAAGGTGGGG ACGCCTTTAA GCACCTGTGC CATCAGCGCC GGCGCCAGGA GTTGGAGGCC 
CAGAACCGCG CCTTCGAGGA GGAGCAGAAG CGCAGGAAGC AGGAGATTGT GAGTCGGCTC 
TTGAAGGAAG AGGCTGTGGA GGACCGGAGG AGGCCACGGC CCGAGCCCCC GAGACCCGCA 
GGCCAGCGGA GCCTGAGGGA CCAGACCTGG AGCTACGTGG CCACCTTCTT CGATGGGCAG 
AGCACAGAGG CCCCGCTCTG CTGTCCCCTG CCTGATGTCC CTGGACAGAG GCCAGGTCCA 
GCCCAAGAGC AGATAAAGCT CCAGGAGACC AGGTCCAGAT TCTACCAGTC CTCTCCGCTG 
CTGACGGCCA TCTCCAGTGA GTCTGTCCAG GGGGACGGGA GCCTTGGCGG GGAGGACACA 
CTGGCTTGGC CGGAGATCCC CGGGCTCTGG AAGGAGGACT ACAAGCCTTT CCAGGTATCC 
AAGGAGGATG TGGACCGGAA GCCAGTGGGC GGAACAAAGA TGGACAAGGA CATCCTGGCG 
CGGACAATGC AACGGCTACG CAGTGGGGTG ATCCACAAAC AGGTGGCATC AGGGCGTGAG 
TTCAGAGGGT GCCCCTTCAA CAGCAAGCCC AAGTTCATCC ACTTCAAGGA CTTTGATGTG 
GGCAAAGTGT ACAAGAAGAA GATCACGCTG ATCAACGCCA CCTACACGAT TAACTACTGC 
AAGCTGGTGG GCGTGGATGA GAGCCTGCAG GACTTCATCC ACGTGGACTT TGACCCCCCA 
GGCCCACTAT CAGCCGGCAT GTCCTGCGAA GTGCTTGTCA CCTTCAAGCC CATGATAAAC 
AAAGATCTGG AAGGAAATGT CTCGTTTCTG GCTCAGACAG GCGGATTTTC TGTTCCACTG 
AAATGTTCAA CAAAGAAATG CTCTCTGTCC CTCGACAAGG AGCTCATCGA CTTTGGCAGC 
TACACAGTGG GTGAGACTGC AATCCGGACC ATCACGCTGA CCAACACTGG GGGCTTGGGC 
ACCAAGTTCA GATTTCTTCT GGCATCAGAG ACTTGTGAGA TGGACACCTC CCAGTCGGTC 
CTAAAATTAA GTAGTACCTT CACGTATGAA GACAAAAGTT TGTATGATAA ATTCACCACC 
AGTTTTTCTG AGCATCAGAT GGAGGGCAAT GAGTCATCTC CTACAGATGT ACCAAGCCAG 
AAGGAGTCTG GAAAGCTGGA CGTGAAGGAG CAGGAGGAGG GGCACCCCAT CGAATCAGAG 
GGCGTGACCT TGACCACCAT CATGACTATA CCTCTCAATG AGGAACAGAT GGAGTTCTCG 
CTGGGAGAGA CAACAGAGGG GGACATAGGC CCCTTCAGCT CCATCAAGGT GCCCGTCATC 
TTCACCCCGA TTGTCCCGGG AACGGTCCAG AACAGTTTCA AGGTTGTGTT TAAGAATCAG 
CAGTGCCCAA CTTTATATTT CAAAGTCGTC GGCGTGGCTA TTGATGTGCC TGTCTGGGTG 
CCAAAGCCCA ACGTAGACCT GAAGATCTGC ATGTACGATC GGCTGTACCA GGACTCGATC 
CTCGTCCACA CGCGGTCGAA GGCCGCGTTG CGCCTAAAGT TCGAGGTGTG CAAGGAGCTG 
CGGGGCCACG TGGAGCTCCT GCCTGAGACT GGCTACATCC AGGCCCTGTC GTCCTACTCA 
GTGCAGCTCA AGTTCCTGCC CAGACACTCC CTCCCAGAAG ATGCAGGGAA GTATTTTGAC 
AAGGAGACCA GGGTTCTGGA GGCCCCGATG ACTATCCGGG TGGCTGACCA GATCAAACCC 
GTGGGGTTTA CTGTCCATGC TGTAGTCACC ACCTCAGACC TGGAGCTCAG CCCCGCAGGG 
CTGGACTTTG GCTACTGTAC CATCTACGAG GCCATCAGGA CCCAAATCAG CCTCTGCAAT 
CATTCCCTCC TGCCCCAGGA GTTTGGGTTC GTTGGACTCC CCAAGTTTGT GGACATCCAG 
CCCAACGATG GGTTCGGGAC AATCCTGCCA CTGGAAACGC TACAGCTCGA TGTGATCTTT 
CAGCCCACCA AGGCCAAGGA GTACAACTTT GAGCTCATCT GCAAGTCAGA GATTAATCGG 
TGCTTCAAGC TGGCATGCCG GGCCGTGGGT GTCTACCCAC CCCTGGAGCT GTCCCACAAC 
CAGATCAAGT TTGCAGCCAC CTCCTTGTAC GACACATCCG TGGCCACTCT GTACGTCATC 
AACTCCCACC TGAGCATGAA CTCACTGACC CACTCAGTGC CCCGCATTGG CTCAGAGGAC 
GCTTTTCCCG TGGGGCCCAC GTCTTTTGAG TTCCTGCTGC CCCCAGATTC GCCCATCACC 
ATCTCACCCC TGGTGGGCAC CGTGTGGCCA GGAAAGAGGT GCCTGGTCCG AGTGGCCTTC 
CGGCCAGTGC TGCCCAGCGA GCTGATCCAC CAGGAGGCCT TCCAGGCCCT GAGCAGAAAA 
GCTGAGGTCA AATCGTCCCA AAAGGAGACG GTCACCCAGA GGAAGGACCA GCGGAGACCA 
TCTTTCTCCA TGCTGCGGCT GCAGAACCAA GACCGGCTGC TCCGGGCCTC AGCCTCCCAG 
CCCCCGGAGC TGCAGAAGGA GGACCTTGCA TCCAGCTCTC AAGAGTACCA GTCGGCACAG 
GCCTCCCTGA CCAGGTCTTT CCAGGGGAAG TTTGACAAGT TTGTGGTTCC CTGTGTTGTT 
GCTAGCGGCG ACACCAAAGA CAGGAAGGGG ACAGAACCGC TGAGCTTCAG TCCCCACAAC 
ACGCTGTACC TGGAGCTGTG GTGTCCAGCA GTGGCGCCGT CTATCGTGGT GACGTCCGAC 
AAAGGCAAGA CCATCATTAA CTTCGGCGAC ATTGCTGCAG GACACCGGGG CATAAAGAAG 
GTCTCCATCC AGAACATCTC CCCTGAGGAT CTCGACCTGG AGTACTCCGT GCTGAACCCC 
GATGGCCCCT TCGTCCTGCT GAACCCCACC ATCAGGTTGC CCTCTGGCGA GACCCAGGTC 
CTAGATCTGT CCTTCTCCCC ACGTGAGAGT GTCGTGGCCC AAGAAATACT GGACATCATC 
ACCAAGAAAG GCACGCTCTC CCTGGCCCTC ATGGGCACGG GTGTGGCGTC CACGATCACG 
TGCTCCATTG AAGGTGATGT CCTCAACATG GGCTACGTGA TCGCCAGAGA GTCTGTCTCC 
TTGGGCTTCA AGCTGCAGAA CAACTCCCCG CTGCCCATCA AGTTCTCCAT GCAGCTGGAC 
AGCCTCTCCT CCAGGAGCAG AGACCAGCAC CGCCTGCCAC AGTTCCTGGC CTCGCCTGAC 
CAGAGGACGG AAGTCGTGGG CACGCAGAAC TACAGTGGCC AGAGCGTGTT CAGCGTGGTC 
CCGGTCGAGG GCGTCATGGA GCCCGGCAAG GCTCAGGACT TCACTGTGAC CTTCAGCCCA 
GACCACGAGA GCCTATACTT CTCTGACCGG CTGCAGGTCG TGCTCTTTGA AAAGGCAGGA 
CTCCGACCCA GCCTGGACCC CTCCTCCCCA CCCGCATGTC CATTTGTCCA AGCCCTTTGG 
CTGCCTGAGC GGCCCCACAT TGGAGCTTGG TGCCTGGGGG GGCTCCACAT CAGGCTGGGG 
GTCATCAGGT GGAGAGACCA ATGTCTGGGG TCCCACAGGC CCACATCACA GCCCCGCCCC 
CTCCCAGAGC CCCACATGCA GGGTTGCAAA CCGCTGTGCT CCTGCCCCCA GAAAGTGTCC 
CACCACATCC TCCTGAAGGG CGCGGCCCGC GAGCACATGA TGTTCGTGGA GGGCGGAGAC 
CCCCTGGACG TGCCCGTGGA GTCCCTGACC GTGATCCCTG CCTTTGACCT GGAGCACAGA 
GAGGGTGAGC CCTCTGAGGG CAGAGAGCCC CGAGGTGGGC AAGGTGGCAG AAGAGGACAC 
ATGACCTTTG GAGTCTGCAG GGAGGGCAGG CATTCCTGGC CCATCCACCA CAGAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CFAP74 ( XM_024976795.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024832563.1 Bos taurus cilia and flagella associated protein 74 (CFAP74), transcript variant X6, mRNA.

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CloneID	OBa56332
Clone ID Related Accession (Same CDS sequence)	XM_024976795.1
Accession Version	XM_024976795.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4917bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	cilia- and flagella-associated protein 74 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAAG ACGACAAGAA GCTTTTGGAT GACAAGCCGC TCGTGGAAGA TGACACAGAG 
GAGCTGGAGG ACCCAGAGCT TGTGCTGGAC TGTGTCCCCA GGGAGGCTGA GCAGAAGGCC 
GGCCTGGGAA GCTCCTGTCC AGGAATAGAG CAGAGCAAGG ACAAGCAGAA GCAGAGGTTG 
ACTGCTCAGG AGAAAAGGCA GGCCTGCCTG CTGCGCAGGG ACTTGAGCCA CCTGGACCAG 
CTGCATGAAG AGAAAGACCT TTTCATCCAG AAGACAAGGG GGGAGCTACG TGCCTGCCGC 
AGGAGGATGG AACTCCTAGT CAAGCAGCAG GAGAGTGTGG CAGCCGAGAT AGCTGCAGAG 
AGGGCAGCCA ACAACATGGC AGCCATGGGC CGCCTGGAGG CAGCGTCGAG GCGCCTGTGC 
TCAGAGCTGA AGAACGAGCA GGACCTGCAG GCGCAGATGA TGGCGGTGCT GCAGCAGAGC 
CAGAGCGCCC TGTGGCAGAT TGAGGTCCAG GAGGGGCAGC TAGAGGACGC CCGCAAGTCG 
GCCCAAGAGG AGGCTGCAGC CGCCAGGAGA GGCCTTCAGG AGCGGGCAGC CAAGCAGCTG 
CACCGGGAGA TGGGGGCCTC GGGGAAGGCA GAGCGGAACA GGCTGCTGCG AGCCCGGAGA 
TGCGTGCACC TGCAGAAGGA GCTTGGACTC CGGCAGCAGA AGCTGGTGGA GGAAGCCCAG 
AAGAACCACC GGAAGGCCCT GAGGTTCCTG AAGGCCTCGT TGGGGAGGAT CCGTGAGCAG 
GAGCAGAAGG AGGAGCTGGA GACCCGAGAG CACATGCGCA GGCGCATGGA CGCGGTGCTG 
GCCCTGAAGA ACAGTATCTC CGCCAACCGG GAAACGCTCC GGAAATTTCA AGCATGGGGC 
CAGGCCAAGA CAGAGCTGGC AGAACAGAAG GCCCAGGCGG AGAAGAAGGC AATTCTGGCC 
CAAGGTGGGG ACGCCTTTAA GCACCTGTGC CATCAGCGCC GGCGCCAGGA GTTGGAGGCC 
CAGAACCGCG CCTTCGAGGA GGAGCAGAAG CGCAGGAAGC AGGAGATTGT GAGTCGGCTC 
TTGAAGGAAG AGGCTGTGGA GGACCGGAGG AGGCCACGGC CCGAGCCCCC GAGACCCGCA 
GGCCAGCGGA GCCTGAGGGA CCAGACCTGG AGCTACGTGG CCACCTTCTT CGATGGGCAG 
AGCACAGAGG CCCCGCTCTG CTGTCCCCTG CCTGATGTCC CTGGACAGAG GCCAGGTCCA 
GCCCAAGAGC AGATAAAGCT CCAGGAGACC AGGTCCAGAT TCTACCAGTC CTCTCCGCTG 
CTGACGGCCA TCTCCAGTGA GTCTGTCCAG GGGGACGGGA GCCTTGGCGG GGAGGACACA 
CTGGCTTGGC CGGAGATCCC CGGGCTCTGG AAGGAGGACT ACAAGCCTTT CCAGGTATCC 
AAGGAGGATG TGGACCGGAA GCCAGTGGGC GGAACAAAGA TGGACAAGGA CATCCTGGCG 
CGGACAATGC AACGGCTACG CAGTGGGGTG ATCCACAAAC AGGTGGCATC AGGGCGTGAG 
TTCAGAGGGT GCCCCTTCAA CAGCAAGCCC AAGTTCATCC ACTTCAAGGA CTTTGATGTG 
GGCAAAGTGT ACAAGAAGAA GATCACGCTG ATCAACGCCA CCTACACGAT TAACTACTGC 
AAGCTGGTGG GCGTGGATGA GAGCCTGCAG GACTTCATCC ACGTGGACTT TGACCCCCCA 
GGCCCACTAT CAGCCGGCAT GTCCTGCGAA GTGCTTGTCA CCTTCAAGCC CATGATAAAC 
AAAGATCTGG AAGGAAATGT CTCGTTTCTG GCTCAGACAG GCGGATTTTC TGTTCCACTG 
AAATGTTCAA CAAAGAAATG CTCTCTGTCC CTCGACAAGG AGCTCATCGA CTTTGGCAGC 
TACACAGTGG GTGAGACTGC AATCCGGACC ATCACGCTGA CCAACACTGG GGGCTTGGGC 
ACCAAGTTCA GATTTCTTCT GGCATCAGAG ACTTGTGAGA TGGACACCTC CCAGTCGGTC 
CTAAAATTAA GTAGTACCTT CACGTATGAA GACAAAAGTT TGTATGATAA ATTCACCACC 
AGTTTTTCTG AGCATCAGAT GGAGGGCAAT GAGTCATCTC CTACAGATGT ACCAAGCCAG 
AAGGAGTCTG GAAAGCTGGA CGTGAAGGAG CAGGAGGAGG GGCACCCCAT CGAATCAGAG 
GGCGTGACCT TGACCACCAT CATGACTATA CCTCTCAATG AGGAACAGAT GGAGTTCTCG 
CTGGGAGAGA CAACAGAGGG GGACATAGGC CCCTTCAGCT CCATCAAGGT GCCCGTCATC 
TTCACCCCGA TTGTCCCGGG AACGGTCCAG AACAGTTTCA AGGTTGTGTT TAAGAATCAG 
CAGTGCCCAA CTTTATATTT CAAAGTCGTC GGCGTGGCTA TTGATGTGCC TGTCTGGGTG 
CCAAAGCCCA ACGTAGACCT GAAGATCTGC ATGTACGATC GGCTGTACCA GGACTCGATC 
CTCGTCCACA CGCGGTCGAA GGCCGCGTTG CGCCTAAAGT TCGAGGTGTG CAAGGAGCTG 
CGGGGCCACG TGGAGCTCCT GCCTGAGACT GGCTACATCC AGGCCCTGTC GTCCTACTCA 
GTGCAGCTCA AGTTCCTGCC CAGACACTCC CTCCCAGAAG ATGCAGGGAA GTATTTTGAC 
AAGGAGACCA GGGTTCTGGA GGCCCCGATG ACTATCCGGG TGGCTGACCA GATCAAACCC 
GTGGGGTTTA CTGTCCATGC TGTAGTCACC ACCTCAGACC TGGAGCTCAG CCCCGCAGGG 
CTGGACTTTG GCTACTGTAC CATCTACGAG GCCATCAGGA CCCAAATCAG CCTCTGCAAT 
CATTCCCTCC TGCCCCAGGA GTTTGGGTTC GTTGGACTCC CCAAGTTTGT GGACATCCAG 
CCCAACGATG GGTTCGGGAC AATCCTGCCA CTGGAAACGC TACAGCTCGA TGTGATCTTT 
CAGCCCACCA AGGCCAAGGA GTACAACTTT GAGCTCATCT GCAAGTCAGA GATTAATCGG 
TGCTTCAAGC TGGCATGCCG GGCCGTGGGT GTCTACCCAC CCCTGGAGCT GTCCCACAAC 
CAGATCAAGT TTGCAGCCAC CTCCTTGTAC GACACATCCG TGGCCACTCT GTACGTCATC 
AACTCCCACC TGAGCATGAA CTCACTGACC CACTCAGTGC CCCGCATTGG CTCAGAGGAC 
GCTTTTCCCG TGGGGCCCAC GTCTTTTGAG TTCCTGCTGC CCCCAGATTC GCCCATCACC 
ATCTCACCCC TGGTGGGCAC CGTGTGGCCA GGAAAGAGGT GCCTGGTCCG AGTGGCCTTC 
CGGCCAGTGC TGCCCAGCGA GCTGATCCAC CAGGAGGCCT TCCAGGCCCT GAGCAGAAAA 
GCTGAGGTCA AATCGTCCCA AAAGGAGACG GTCACCCAGA GGAAGGACCA GCGGAGACCA 
TCTTTCTCCA TGCTGCGGCT GCAGAACCAA GACCGGCTGC TCCGGGCCTC AGCCTCCCAG 
CCCCCGGAGC TGCAGAAGGA GGACCTTGCA TCCAGCTCTC AAGAGTACCA GTCGGCACAG 
GCCTCCCTGA CCAGGTCTTT CCAGGGGAAG TTTGACAAGT TTGTGGTTCC CTGTGTTGTT 
GCTAGCGGCG ACACCAAAGA CAGGAAGGGG ACAGAACCGC TGAGCTTCAG TCCCCACAAC 
ACGCTGTACC TGGAGCTGTG GTGTCCAGCA GTGGCGCCGT CTATCGTGGT GACGTCCGAC 
AAAGGCAAGA CCATCATTAA CTTCGGCGAC ATTGCTGCAG GACACCGGGG CATAAAGAAG 
GTCTCCATCC AGAACATCTC CCCTGAGGAT CTCGACCTGG AGTACTCCGT GCTGAACCCC 
GATGGCCCCT TCGTCCTGCT GAACCCCACC ATCAGGTTGC CCTCTGGCGA GACCCAGGTC 
CTAGATCTGT CCTTCTCCCC ACGTGAGAGT GTCGTGGCCC AAGAAATACT GGACATCATC 
ACCAAGAAAG GCACGCTCTC CCTGGCCCTC ATGGGCACGG GTGTGGCGTC CACGATCACG 
TGCTCCATTG AAGGTGATGT CCTCAACATG GGCTACGTGA TCGCCAGAGA GTCTGTCTCC 
TTGGGCTTCA AGCTGCAGAA CAACTCCCCG CTGCCCATCA AGTTCTCCAT GCAGCTGGAC 
AGCCTCTCCT CCAGGAGCAG AGACCAGCAC CGCCTGCCAC AGTTCCTGGC CTCGCCTGAC 
CAGAGGACGG AAGTCGTGGG CACGCAGAAC TACAGTGGCC AGAGCGTGTT CAGCGTGGTC 
CCGGTCGAGG GCGTCATGGA GCCCGGCAAG GCTCAGGACT TCACTGTGAC CTTCAGCCCA 
GACCACGAGA GCCTATACTT CTCTGACCGG CTGCAGAAAG TGTCCCACCA CATCCTCCTG 
AAGGGCGCGG CCCGCGAGCA CATGATGTTC GTGGAGGGCG GAGACCCCCT GGACGTGCCC 
GTGGAGTCCC TGACCGTGAT CCCTGCCTTT GACCTGGAGC ACAGAGAGGA AGCTGAGGAG 
CTAAAGCCCA TCCTGGTGAC CCTGGACTAC GCCCAGCTGG ACCCAGACAC ACAGGCCCCA 
CCTGCCACCC GAGAACTGCA GGTGGGCTGC ATCCGGACTA CCCAGCTGTC CGCAAAGAAG 
ACTGTTGAGT TCAGCATGGA CAACATCGCA GCCCTGCAGC ACAAGGGCTT CACCATTGAG 
CCCTCCAGAG GCTCTGTGGA GCGCGGCCAG ACCAAGGCCA TCAGCATCTC CTGGACACCA 
CCCATCGACT TTGATCCGGA CCACCCACTC TTGGCATCGG CCCTGCTGCA GCTCAGGGGG 
GATGTGAAGG AGACCTACAA GGTCCTCTTT GTAGCCCAGG TGGTGACCGG TCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024832563.1
CDS	415..5331
Translation	MEEDDKKLLDDKPLVEDDTEELEDPELVLDCVPREAEQKAGLGSSCPGIEQSKDKQKQRLTAQEKRQACLLRRDLSHLDQLHEEKDLFIQKTRGELRACRRRMELLVKQQESVAAEIAAERAANNMAAMGRLEAASRRLCSELKNEQDLQAQMMAVLQQSQSALWQIEVQEGQLEDARKSAQEEAAAARRGLQERAAKQLHREMGASGKAERNRLLRARRCVHLQKELGLRQQKLVEEAQKNHRKALRFLKASLGRIREQEQKEELETREHMRRRMDAVLALKNSISANRETLRKFQAWGQAKTELAEQKAQAEKKAILAQGGDAFKHLCHQRRRQELEAQNRAFEEEQKRRKQEIVSRLLKEEAVEDRRRPRPEPPRPAGQRSLRDQTWSYVATFFDGQSTEAPLCCPLPDVPGQRPGPAQEQIKLQETRSRFYQSSPLLTAISSESVQGDGSLGGEDTLAWPEIPGLWKEDYKPFQVSKEDVDRKPVGGTKMDKDILARTMQRLRSGVIHKQVASGREFRGCPFNSKPKFIHFKDFDVGKVYKKKITLINATYTINYCKLVGVDESLQDFIHVDFDPPGPLSAGMSCEVLVTFKPMINKDLEGNVSFLAQTGGFSVPLKCSTKKCSLSLDKELIDFGSYTVGETAIRTITLTNTGGLGTKFRFLLASETCEMDTSQSVLKLSSTFTYEDKSLYDKFTTSFSEHQMEGNESSPTDVPSQKESGKLDVKEQEEGHPIESEGVTLTTIMTIPLNEEQMEFSLGETTEGDIGPFSSIKVPVIFTPIVPGTVQNSFKVVFKNQQCPTLYFKVVGVAIDVPVWVPKPNVDLKICMYDRLYQDSILVHTRSKAALRLKFEVCKELRGHVELLPETGYIQALSSYSVQLKFLPRHSLPEDAGKYFDKETRVLEAPMTIRVADQIKPVGFTVHAVVTTSDLELSPAGLDFGYCTIYEAIRTQISLCNHSLLPQEFGFVGLPKFVDIQPNDGFGTILPLETLQLDVIFQPTKAKEYNFELICKSEINRCFKLACRAVGVYPPLELSHNQIKFAATSLYDTSVATLYVINSHLSMNSLTHSVPRIGSEDAFPVGPTSFEFLLPPDSPITISPLVGTVWPGKRCLVRVAFRPVLPSELIHQEAFQALSRKAEVKSSQKETVTQRKDQRRPSFSMLRLQNQDRLLRASASQPPELQKEDLASSSQEYQSAQASLTRSFQGKFDKFVVPCVVASGDTKDRKGTEPLSFSPHNTLYLELWCPAVAPSIVVTSDKGKTIINFGDIAAGHRGIKKVSIQNISPEDLDLEYSVLNPDGPFVLLNPTIRLPSGETQVLDLSFSPRESVVAQEILDIITKKGTLSLALMGTGVASTITCSIEGDVLNMGYVIARESVSLGFKLQNNSPLPIKFSMQLDSLSSRSRDQHRLPQFLASPDQRTEVVGTQNYSGQSVFSVVPVEGVMEPGKAQDFTVTFSPDHESLYFSDRLQKVSHHILLKGAAREHMMFVEGGDPLDVPVESLTVIPAFDLEHREEAEELKPILVTLDYAQLDPDTQAPPATRELQVGCIRTTQLSAKKTVEFSMDNIAALQHKGFTIEPSRGSVERGQTKAISISWTPPIDFDPDHPLLASALLQLRGDVKETYKVLFVAQVVTGP

Target ORF information:

RefSeq Version	XM_024976795.1
Organism	Bos taurus(cattle)
Definition	Bos taurus cilia and flagella associated protein 74 (CFAP74), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024976795.1

ORF Insert Sequence:

ATGGAAGAAG ACGACAAGAA GCTTTTGGAT GACAAGCCGC TCGTGGAAGA TGACACAGAG 
GAGCTGGAGG ACCCAGAGCT TGTGCTGGAC TGTGTCCCCA GGGAGGCTGA GCAGAAGGCC 
GGCCTGGGAA GCTCCTGTCC AGGAATAGAG CAGAGCAAGG ACAAGCAGAA GCAGAGGTTG 
ACTGCTCAGG AGAAAAGGCA GGCCTGCCTG CTGCGCAGGG ACTTGAGCCA CCTGGACCAG 
CTGCATGAAG AGAAAGACCT TTTCATCCAG AAGACAAGGG GGGAGCTACG TGCCTGCCGC 
AGGAGGATGG AACTCCTAGT CAAGCAGCAG GAGAGTGTGG CAGCCGAGAT AGCTGCAGAG 
AGGGCAGCCA ACAACATGGC AGCCATGGGC CGCCTGGAGG CAGCGTCGAG GCGCCTGTGC 
TCAGAGCTGA AGAACGAGCA GGACCTGCAG GCGCAGATGA TGGCGGTGCT GCAGCAGAGC 
CAGAGCGCCC TGTGGCAGAT TGAGGTCCAG GAGGGGCAGC TAGAGGACGC CCGCAAGTCG 
GCCCAAGAGG AGGCTGCAGC CGCCAGGAGA GGCCTTCAGG AGCGGGCAGC CAAGCAGCTG 
CACCGGGAGA TGGGGGCCTC GGGGAAGGCA GAGCGGAACA GGCTGCTGCG AGCCCGGAGA 
TGCGTGCACC TGCAGAAGGA GCTTGGACTC CGGCAGCAGA AGCTGGTGGA GGAAGCCCAG 
AAGAACCACC GGAAGGCCCT GAGGTTCCTG AAGGCCTCGT TGGGGAGGAT CCGTGAGCAG 
GAGCAGAAGG AGGAGCTGGA GACCCGAGAG CACATGCGCA GGCGCATGGA CGCGGTGCTG 
GCCCTGAAGA ACAGTATCTC CGCCAACCGG GAAACGCTCC GGAAATTTCA AGCATGGGGC 
CAGGCCAAGA CAGAGCTGGC AGAACAGAAG GCCCAGGCGG AGAAGAAGGC AATTCTGGCC 
CAAGGTGGGG ACGCCTTTAA GCACCTGTGC CATCAGCGCC GGCGCCAGGA GTTGGAGGCC 
CAGAACCGCG CCTTCGAGGA GGAGCAGAAG CGCAGGAAGC AGGAGATTGT GAGTCGGCTC 
TTGAAGGAAG AGGCTGTGGA GGACCGGAGG AGGCCACGGC CCGAGCCCCC GAGACCCGCA 
GGCCAGCGGA GCCTGAGGGA CCAGACCTGG AGCTACGTGG CCACCTTCTT CGATGGGCAG 
AGCACAGAGG CCCCGCTCTG CTGTCCCCTG CCTGATGTCC CTGGACAGAG GCCAGGTCCA 
GCCCAAGAGC AGATAAAGCT CCAGGAGACC AGGTCCAGAT TCTACCAGTC CTCTCCGCTG 
CTGACGGCCA TCTCCAGTGA GTCTGTCCAG GGGGACGGGA GCCTTGGCGG GGAGGACACA 
CTGGCTTGGC CGGAGATCCC CGGGCTCTGG AAGGAGGACT ACAAGCCTTT CCAGGTATCC 
AAGGAGGATG TGGACCGGAA GCCAGTGGGC GGAACAAAGA TGGACAAGGA CATCCTGGCG 
CGGACAATGC AACGGCTACG CAGTGGGGTG ATCCACAAAC AGGTGGCATC AGGGCGTGAG 
TTCAGAGGGT GCCCCTTCAA CAGCAAGCCC AAGTTCATCC ACTTCAAGGA CTTTGATGTG 
GGCAAAGTGT ACAAGAAGAA GATCACGCTG ATCAACGCCA CCTACACGAT TAACTACTGC 
AAGCTGGTGG GCGTGGATGA GAGCCTGCAG GACTTCATCC ACGTGGACTT TGACCCCCCA 
GGCCCACTAT CAGCCGGCAT GTCCTGCGAA GTGCTTGTCA CCTTCAAGCC CATGATAAAC 
AAAGATCTGG AAGGAAATGT CTCGTTTCTG GCTCAGACAG GCGGATTTTC TGTTCCACTG 
AAATGTTCAA CAAAGAAATG CTCTCTGTCC CTCGACAAGG AGCTCATCGA CTTTGGCAGC 
TACACAGTGG GTGAGACTGC AATCCGGACC ATCACGCTGA CCAACACTGG GGGCTTGGGC 
ACCAAGTTCA GATTTCTTCT GGCATCAGAG ACTTGTGAGA TGGACACCTC CCAGTCGGTC 
CTAAAATTAA GTAGTACCTT CACGTATGAA GACAAAAGTT TGTATGATAA ATTCACCACC 
AGTTTTTCTG AGCATCAGAT GGAGGGCAAT GAGTCATCTC CTACAGATGT ACCAAGCCAG 
AAGGAGTCTG GAAAGCTGGA CGTGAAGGAG CAGGAGGAGG GGCACCCCAT CGAATCAGAG 
GGCGTGACCT TGACCACCAT CATGACTATA CCTCTCAATG AGGAACAGAT GGAGTTCTCG 
CTGGGAGAGA CAACAGAGGG GGACATAGGC CCCTTCAGCT CCATCAAGGT GCCCGTCATC 
TTCACCCCGA TTGTCCCGGG AACGGTCCAG AACAGTTTCA AGGTTGTGTT TAAGAATCAG 
CAGTGCCCAA CTTTATATTT CAAAGTCGTC GGCGTGGCTA TTGATGTGCC TGTCTGGGTG 
CCAAAGCCCA ACGTAGACCT GAAGATCTGC ATGTACGATC GGCTGTACCA GGACTCGATC 
CTCGTCCACA CGCGGTCGAA GGCCGCGTTG CGCCTAAAGT TCGAGGTGTG CAAGGAGCTG 
CGGGGCCACG TGGAGCTCCT GCCTGAGACT GGCTACATCC AGGCCCTGTC GTCCTACTCA 
GTGCAGCTCA AGTTCCTGCC CAGACACTCC CTCCCAGAAG ATGCAGGGAA GTATTTTGAC 
AAGGAGACCA GGGTTCTGGA GGCCCCGATG ACTATCCGGG TGGCTGACCA GATCAAACCC 
GTGGGGTTTA CTGTCCATGC TGTAGTCACC ACCTCAGACC TGGAGCTCAG CCCCGCAGGG 
CTGGACTTTG GCTACTGTAC CATCTACGAG GCCATCAGGA CCCAAATCAG CCTCTGCAAT 
CATTCCCTCC TGCCCCAGGA GTTTGGGTTC GTTGGACTCC CCAAGTTTGT GGACATCCAG 
CCCAACGATG GGTTCGGGAC AATCCTGCCA CTGGAAACGC TACAGCTCGA TGTGATCTTT 
CAGCCCACCA AGGCCAAGGA GTACAACTTT GAGCTCATCT GCAAGTCAGA GATTAATCGG 
TGCTTCAAGC TGGCATGCCG GGCCGTGGGT GTCTACCCAC CCCTGGAGCT GTCCCACAAC 
CAGATCAAGT TTGCAGCCAC CTCCTTGTAC GACACATCCG TGGCCACTCT GTACGTCATC 
AACTCCCACC TGAGCATGAA CTCACTGACC CACTCAGTGC CCCGCATTGG CTCAGAGGAC 
GCTTTTCCCG TGGGGCCCAC GTCTTTTGAG TTCCTGCTGC CCCCAGATTC GCCCATCACC 
ATCTCACCCC TGGTGGGCAC CGTGTGGCCA GGAAAGAGGT GCCTGGTCCG AGTGGCCTTC 
CGGCCAGTGC TGCCCAGCGA GCTGATCCAC CAGGAGGCCT TCCAGGCCCT GAGCAGAAAA 
GCTGAGGTCA AATCGTCCCA AAAGGAGACG GTCACCCAGA GGAAGGACCA GCGGAGACCA 
TCTTTCTCCA TGCTGCGGCT GCAGAACCAA GACCGGCTGC TCCGGGCCTC AGCCTCCCAG 
CCCCCGGAGC TGCAGAAGGA GGACCTTGCA TCCAGCTCTC AAGAGTACCA GTCGGCACAG 
GCCTCCCTGA CCAGGTCTTT CCAGGGGAAG TTTGACAAGT TTGTGGTTCC CTGTGTTGTT 
GCTAGCGGCG ACACCAAAGA CAGGAAGGGG ACAGAACCGC TGAGCTTCAG TCCCCACAAC 
ACGCTGTACC TGGAGCTGTG GTGTCCAGCA GTGGCGCCGT CTATCGTGGT GACGTCCGAC 
AAAGGCAAGA CCATCATTAA CTTCGGCGAC ATTGCTGCAG GACACCGGGG CATAAAGAAG 
GTCTCCATCC AGAACATCTC CCCTGAGGAT CTCGACCTGG AGTACTCCGT GCTGAACCCC 
GATGGCCCCT TCGTCCTGCT GAACCCCACC ATCAGGTTGC CCTCTGGCGA GACCCAGGTC 
CTAGATCTGT CCTTCTCCCC ACGTGAGAGT GTCGTGGCCC AAGAAATACT GGACATCATC 
ACCAAGAAAG GCACGCTCTC CCTGGCCCTC ATGGGCACGG GTGTGGCGTC CACGATCACG 
TGCTCCATTG AAGGTGATGT CCTCAACATG GGCTACGTGA TCGCCAGAGA GTCTGTCTCC 
TTGGGCTTCA AGCTGCAGAA CAACTCCCCG CTGCCCATCA AGTTCTCCAT GCAGCTGGAC 
AGCCTCTCCT CCAGGAGCAG AGACCAGCAC CGCCTGCCAC AGTTCCTGGC CTCGCCTGAC 
CAGAGGACGG AAGTCGTGGG CACGCAGAAC TACAGTGGCC AGAGCGTGTT CAGCGTGGTC 
CCGGTCGAGG GCGTCATGGA GCCCGGCAAG GCTCAGGACT TCACTGTGAC CTTCAGCCCA 
GACCACGAGA GCCTATACTT CTCTGACCGG CTGCAGAAAG TGTCCCACCA CATCCTCCTG 
AAGGGCGCGG CCCGCGAGCA CATGATGTTC GTGGAGGGCG GAGACCCCCT GGACGTGCCC 
GTGGAGTCCC TGACCGTGAT CCCTGCCTTT GACCTGGAGC ACAGAGAGGA AGCTGAGGAG 
CTAAAGCCCA TCCTGGTGAC CCTGGACTAC GCCCAGCTGG ACCCAGACAC ACAGGCCCCA 
CCTGCCACCC GAGAACTGCA GGTGGGCTGC ATCCGGACTA CCCAGCTGTC CGCAAAGAAG 
ACTGTTGAGT TCAGCATGGA CAACATCGCA GCCCTGCAGC ACAAGGGCTT CACCATTGAG 
CCCTCCAGAG GCTCTGTGGA GCGCGGCCAG ACCAAGGCCA TCAGCATCTC CTGGACACCA 
CCCATCGACT TTGATCCGGA CCACCCACTC TTGGCATCGG CCCTGCTGCA GCTCAGGGGG 
GATGTGAAGG AGACCTACAA GGTCCTCTTT GTAGCCCAGG TGGTGACCGG TCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CFAP74 ( XM_015466809.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015322295.1 Bos taurus cilia and flagella associated protein 74 (CFAP74), mRNA.

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CloneID	OBa93026
Clone ID Related Accession (Same CDS sequence)	XM_015466809.1 , XM_015475230.1
Accession Version	XM_015466809.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5511bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	cilia- and flagella-associated protein 74
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646324.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

ATGGAAGAAG ACGACAAGAA GCTTTTGGAT GACAAGCCGC TCGTGGAAGA TGACACAGAG 
GAGCTGGAGG ACCCAGAGCT TGTGCTGGAC TGTGTCCCCA GGGAGGCTGA GCAGAAGGCC 
GGCCTGGGAA GCTCCTGTCC AGGAATAGAG CAGAGCAAGG ACAAGCAGAA GCAGAGGTTG 
ACTGCTCAGG AGAAAAGGCA GGCCTGCCTG CTGCGCAGGG ACTTGAGCCA CCTGGACCAG 
CTGCATGAAG AGAAAGACCT TTTCATCCAG AAGACAAGGG GGGAGCTACG TGCCTGCCGC 
AGGAGGATGG AACTCCTAGT CAAGCAGCAG GAGAGTGTGG CAGCCGAGAT AGCTGCAGAG 
AGGGCAGCCA ACAACATGGC AGCCATGGGC CGCCTGGAGG CAGCGTCGAG GCGCCTGTGC 
TCAGAGCTGA AGAACGAGCA GGACCTGCAG GCGCAGATGA TGGCGGTGCT GCAGCAGAGC 
CAGAGCGCCC TGTGGCAGAT TGAGGTCCAG GAGGGGCAGC TAGAGGACGC CCGCAAGTCG 
GCCCAAGAGG AGGCTGCAGC CGCCAGGAGA GGCCTTCAGG AGCGGGCAGC CAAGCAGCTG 
CACCGGGAGA TGGGGGCCTC GGGGAAGGCA GAGCGGAACA GGCTGCTGCG AGCCCGGAGA 
TGCGTGCACC TGCAGAAGGA GCTTGGACTC CGGCAGCAGA AGCTGGTGGA GGAAGCCCAG 
AAGAACCACC GGAAGGCCCT GAGGTTCCTG AAGGCCTCGT TGGGGAGGAT CCGTGAGCAG 
GAGCAGAAGG AGGAGCTGGA GACCCGAGAG CACATGCGCA GGCGCATGGA CGCGGTGCTG 
GCCCTGAAGA ACAGTATCTC CGCCAACCGG GAAACGCTCC GGAAATTTCA AGCATGGGGC 
CAGGCCAAGA CAGAGCTGGC AGAACAGAAG GCCCAGGCGG AGAAGAAGGC AATTCTGGCC 
CAAGGTGGGG ACGCCTTTAA GCACCTGTGC CATCAGCGCC GGCGCCAGGA GTTGGAGGCC 
CAGAACCGCG CCTTCGAGGA GGAGCAGAAG CGCAGGAAGC AGGAGATTGT GAGTCGGCTC 
TTGAAGGAAG AGGCTGTGGA GGACCGGAGG AGGCCACGGC CCGAGCCCCC GAGACCCGCA 
GGCCAGCGGA GCCTGAGGGA CCAGACCTGG AGCTACGTGG CCACCTTCTT CGATGGGCAG 
AGCACAGAGG CCCCGCTCTG CTGTCCCCTG GTGAGCGGAT GCCCTGGCGG CAACAGGACC 
ATGCCTGCCA GCCAAGATCA GCACCACGGA GAGCGCCCTG GCCCCAGAAC CTTTACTTCC 
TGCTTGCGTC TGATGCCCCA CCCCCACCAC CAGGGCCTTG GATGGCCTCA AGGCTCCCTC 
CCAAGGCAGG CGGGTGGCAG CCTCCCGGGG CTCTGGTGCC TCACCCACTA TCGTCTCGGG 
CCGCCTTACA TATGTGGGAC AGGGATCTTG GGACCCTGTC CTGAGCCCGG TGGCCCTTCC 
ACTTCTGCCC TGTGCATCCC CAACCTGAAG GCCCAGCTCA CTGGCTCTAA CAAGGGTGCT 
GCCGGCTGGG CCCCCAGGCT TCATTGCATG TTGCCCCCCA GGGTCTCCAC TCCTCTCAGT 
GCCCCTCTCC ACCCTGCTGT CCTGGGCAGC GCCCGCCTGC ACCTCCTGCT GCCTCCTCCC 
TATTGTCTCA CCAGCCAGAG TGCTCCTGCT CTTCTCCGCG GGGGGCCTTT CTCTCACCTG 
CAGCAACGTC AATCTCCACC CTTATCCCTA TTCCAGCCTG ATGTCCCTGG ACAGAGGCCA 
GGTCCAGCCC AAGAGCAGAT AAAGCTCCAG GAGACCAGGT CCAGATTCTA CCAGTCCTCT 
CCGCTGCTGA CGGCCATCTC CAGTGAGTCT GTCCAGGGGG ACGGGAGCCT TGGCGGGGAG 
GACACACTGG CTTGGCCGGA GATCCCCGGG CTCTGGAAGG AGGACTACAA GCCTTTCCAG 
GTATCCAAGG AGGATGTGGA CCGGAAGCCA GTGGGCGGAA CAAAGATGGA CAAGGACATC 
CTGGCGCGGA CAATGCAACG GCTACGCAGT GGGGTGATCC ACAAACAGGT GGCATCAGGG 
CGTGAGTTCA GAGGGTGCCC CTTCAACAGC AAGCCCAAGT TCATCCACTT CAAGGACTTT 
GATGTGGGCA AAGTGTACAA GAAGAAGATC ACGCTGATCA ACGCCACCTA CACGATTAAC 
TACTGCAAGC TGGTGGGCGT GGATGAGAGC CTGCAGGACT TCATCCACGT GGACTTTGAC 
CCCCCAGGCC CACTATCAGC CGGCATGTCC TGCGAAGTGC TTGTCACCTT CAAGCCCATG 
ATAAACAAAG ATCTGGAAGG AAATGTCTCG TTTCTGGCTC AGACAGGCGG ATTTTCTGTT 
CCACTGAAAT GTTCAACAAA GAAATGCTCT CTGTCCCTCG ACAAGGAGCT CATCGACTTT 
GGCAGCTACA CAGTGGGTGA GACTGCAATC CGGACCATCA CGCTGACCAA CACTGGGGGC 
TTGGGCACCA AGTTCAGATT TCTTCTGGCA TCAGAGACTT GTGAGATGGA CACCTCCCAG 
TCGGTCCTAA AATTAAGTAG TACCTTCACG TATGAAGACA AAAGTTTGTA TGATAAATTC 
ACCACCAGTT TTTCTGAGCA TCAGATGGAG GGCAATGAGT CATCTCCTAC AGATGTACCA 
AGCCAGAAGG AGTCTGGAAA GCTGGACGTG AAGGAGCAGG AGGAGGGGCA CCCCATCGAA 
TCAGAGGGCG TGACCTTGAC CACCATCATG ACTATACCTC TCAATGAGGA ACAGATGGAG 
TTCTCGCTGG GAGAGACAAC AGAGGGGGAC ATAGGCCCCT TCAGCTCCAT CAAGGTGCCC 
GTCATCTTCA CCCCGATTGT CCCGGGAACG GTCCAGAACA GTTTCAAGGT TGTGTTTAAG 
AATCAGCAGT GCCCAACTTT ATATTTCAAA GTCGTCGGCG TGGCTATTGA TGTGCCTGTC 
TGGGTGCCAA AGCCCAACGT AGACCTGAAG ATCTGCATGT ACGATCGGCT GTACCAGGAC 
TCGATCCTCG TCCACACGCG GTCGAAGGCC GCGTTGCGCC TAAAGTTCGA GGTGTGCAAG 
GAGCTGCGGG GCCACGTGGA GCTCCTGCCT GAGACTGGCT ACATCCAGGC CCTGTCGTCC 
TACTCAGTGC AGCTCAAGTT CCTGCCCAGA CACTCCCTCC CAGAAGATGC AGGGAAGTAT 
TTTGACAAGG AGACCAGGGT TCTGGAGGCC CCGATGACTA TCCGGGTGGC TGACCAGATC 
AAACCCGTGG GGTTTACTGT CCATGCTGTA GTCACCACCT CAGACCTGGA GCTCAGCCCC 
GCAGGGCTGG ACTTTGGCTA CTGTACCATC TACGAGGCCA TCAGGACCCA AATCAGCCTC 
TGCAATCATT CCCTCCTGCC CCAGGAGTTT GGGTTCGTTG GACTCCCCAA GTTTGTGGAC 
ATCCAGCCCA ACGATGGGTT CGGGACAATC CTGCCACTGG AAACGCTACA GCTCGATGTG 
ATCTTTCAGC CCACCAAGGC CAAGGAGTAC AACTTTGAGC TCATCTGCAA GTCAGAGATT 
AATCGGTGCT TCAAGCTGGC ATGCCGGGCC GTGGGTGTCT ACCCACCCCT GGAGCTGTCC 
CACAACCAGA TCAAGTTTGC AGCCACCTCC TTGTACGACA CATCCGTGGC CACTCTGTAC 
GTCATCAACT CCCACCTGAG CATGAACTCA CTGACCCACT CAGTGCCCCG CATTGGCTCA 
GAGGACGCTT TTCCCGTGGG GCCCACGTCT TTTGAGTTCC TGCTGCCCCC AGATTCGCCC 
ATCACCATCT CACCCCTGGT GGGCACCGTG TGGCCAGGAA AGAGGTGCCT GGTCCGAGTG 
GCCTTCCGGC CAGTGCTGCC CAGCGAGCTG ATCCACCAGG AGGCCTTCCA GGCCCTGAGC 
AGAAAAGCTG AGGTCAAATC GTCCCAAAAG GAGACGGTCA CCCAGAGGAA GGACCAGCGG 
AGACCATCTT TCTCCATGCT GCGGCTGCAG AACCAAGACC GGCTGCTCCG GGCCTCAGCC 
TCCCAGCCCC CGGAGCTGCA GAAGGAGGAC CTTGCATCCA GCTCTCAAGA GTACCAGTCG 
GCACAGGCCT CCCTGACCAG GTCTTTCCAG GGGAAGTTTG ACAAGTTTGT GGTTCCCTGT 
GTTGTTGCTA GCGGCGACAC CAAAGACAGG AAGGGGACAG AACCGCTGAG CTTCAGTCCC 
CACAACACGC TGTACCTGGA GCTGTGGTGT CCAGCAGTGG CGCCGTCTAT CGTGGTGACG 
TCCGACAAAG GCAAGACCAT CATTAACTTC GGCGACATTG CTGCAGGACA CCGGGGCATA 
AAGAAGGTCT CCATCCAGAA CATCTCCCCT GAGGATCTCG ACCTGGAGTA CTCCGTGCTG 
AACCCCGATG GCCCCTTCGT CCTGCTGAAC CCCACCATCA GGTTGCCCTC TGGCGAGACC 
CAGGTCCTAG ATCTGTCCTT CTCCCCACGT GAGAGTGTCG TGGCCCAAGA AATACTGGAC 
ATCATCACCA AGAAAGGCAC GCTCTCCCTG GCCCTCATGG GCACGGGTGT GGCGTCCACG 
ATCACGTGCT CCATTGAAGG TGATGTCCTC AACATGGGCT ACGTGATCGC CAGAGAGTCT 
GTCTCCTTGG GCTTCAAGCT GCAGAACAAC TCCCCGCTGC CCATCAAGTT CTCCATGCAG 
CTGGACAGCC TCTCCTCCAG GAGCAGAGAC CAGCACCGCC TGCCACAGTT CCTGGCCTCG 
CCTGACCAGA GGACGGAAGT CGTGGGCACG CAGAACTACA GTGGCCAGAG CGTGTTCAGC 
GTGGTCCCGG TCGAGGGCGT CATGGAGCCC GGCAAGGCTC AGGACTTCAC TGTGACCTTC 
AGCCCAGACC ACGAGAGCCT ATACTTCTCT GACCGGCTGC AGGTCGTGCT CTTTGAAAAG 
AAAGTGTCCC ACCACATCCT CCTGAAGGGC GCGGCCCGCG AGCACATGAT GTTCGTGGAG 
GGCGGAGACC CCCTGGACGT GCCCGTGGAG TCCCTGACCG TGATCCCTGC CTTTGACCTG 
GAGCACAGAG AGGGTGAACC CCCACCAGGC CCTCCTTCTC CAGAAGCTGA GGAGCTAAAG 
CCCATCCTGG TGACCCTGGA CTACGCCCAG CTGGACCCAG ACACACAGGC CCCACCTGCC 
ACCCGAGAAC TGCAGGTGGG CTGCATCCGG ACTACCCAGC TGTCCGCAAA GAAGACTGTT 
GAGTTCAGCA TGGACAACAT CGCAGCCCTG CAGCACAAGG GCTTCACCAT TGAGCCCTCC 
AGAGGCTCTG TGGAGCGCGG CCAGACCAAG GCCATCAGCA TCTCCTGGAC ACCACCCATC 
GACTTTGATC CGGACCACCC ACTCTTGGCA TCGGCCCTGC TGCAGCTCAG GGGGGATGTG 
AAGGAGACCT ACAAGGTCCT CTTTGTAGCC CAGGTGGTGA CCGGTCCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015322295.1
CDS	398..5908
Translation	MEEDDKKLLDDKPLVEDDTEELEDPELVLDCVPREAEQKAGLGSSCPGIEQSKDKQKQRLTAQEKRQACLLRRDLSHLDQLHEEKDLFIQKTRGELRACRRRMELLVKQQESVAAEIAAERAANNMAAMGRLEAASRRLCSELKNEQDLQAQMMAVLQQSQSALWQIEVQEGQLEDARKSAQEEAAAARRGLQERAAKQLHREMGASGKAERNRLLRARRCVHLQKELGLRQQKLVEEAQKNHRKALRFLKASLGRIREQEQKEELETREHMRRRMDAVLALKNSISANRETLRKFQAWGQAKTELAEQKAQAEKKAILAQGGDAFKHLCHQRRRQELEAQNRAFEEEQKRRKQEIVSRLLKEEAVEDRRRPRPEPPRPAGQRSLRDQTWSYVATFFDGQSTEAPLCCPLVSGCPGGNRTMPASQDQHHGERPGPRTFTSCLRLMPHPHHQGLGWPQGSLPRQAGGSLPGLWCLTHYRLGPPYICGTGILGPCPEPGGPSTSALCIPNLKAQLTGSNKGAAGWAPRLHCMLPPRVSTPLSAPLHPAVLGSARLHLLLPPPYCLTSQSAPALLRGGPFSHLQQRQSPPLSLFQPDVPGQRPGPAQEQIKLQETRSRFYQSSPLLTAISSESVQGDGSLGGEDTLAWPEIPGLWKEDYKPFQVSKEDVDRKPVGGTKMDKDILARTMQRLRSGVIHKQVASGREFRGCPFNSKPKFIHFKDFDVGKVYKKKITLINATYTINYCKLVGVDESLQDFIHVDFDPPGPLSAGMSCEVLVTFKPMINKDLEGNVSFLAQTGGFSVPLKCSTKKCSLSLDKELIDFGSYTVGETAIRTITLTNTGGLGTKFRFLLASETCEMDTSQSVLKLSSTFTYEDKSLYDKFTTSFSEHQMEGNESSPTDVPSQKESGKLDVKEQEEGHPIESEGVTLTTIMTIPLNEEQMEFSLGETTEGDIGPFSSIKVPVIFTPIVPGTVQNSFKVVFKNQQCPTLYFKVVGVAIDVPVWVPKPNVDLKICMYDRLYQDSILVHTRSKAALRLKFEVCKELRGHVELLPETGYIQALSSYSVQLKFLPRHSLPEDAGKYFDKETRVLEAPMTIRVADQIKPVGFTVHAVVTTSDLELSPAGLDFGYCTIYEAIRTQISLCNHSLLPQEFGFVGLPKFVDIQPNDGFGTILPLETLQLDVIFQPTKAKEYNFELICKSEINRCFKLACRAVGVYPPLELSHNQIKFAATSLYDTSVATLYVINSHLSMNSLTHSVPRIGSEDAFPVGPTSFEFLLPPDSPITISPLVGTVWPGKRCLVRVAFRPVLPSELIHQEAFQALSRKAEVKSSQKETVTQRKDQRRPSFSMLRLQNQDRLLRASASQPPELQKEDLASSSQEYQSAQASLTRSFQGKFDKFVVPCVVASGDTKDRKGTEPLSFSPHNTLYLELWCPAVAPSIVVTSDKGKTIINFGDIAAGHRGIKKVSIQNISPEDLDLEYSVLNPDGPFVLLNPTIRLPSGETQVLDLSFSPRESVVAQEILDIITKKGTLSLALMGTGVASTITCSIEGDVLNMGYVIARESVSLGFKLQNNSPLPIKFSMQLDSLSSRSRDQHRLPQFLASPDQRTEVVGTQNYSGQSVFSVVPVEGVMEPGKAQDFTVTFSPDHESLYFSDRLQVVLFEKKVSHHILLKGAAREHMMFVEGGDPLDVPVESLTVIPAFDLEHREGEPPPGPPSPEAEELKPILVTLDYAQLDPDTQAPPATRELQVGCIRTTQLSAKKTVEFSMDNIAALQHKGFTIEPSRGSVERGQTKAISISWTPPIDFDPDHPLLASALLQLRGDVKETYKVLFVAQVVTGP

Target ORF information:

RefSeq Version	XM_015466809.1
Organism	Bos taurus(cattle)
Definition	Bos taurus cilia and flagella associated protein 74 (CFAP74), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015466809.1

ORF Insert Sequence:

ATGGAAGAAG ACGACAAGAA GCTTTTGGAT GACAAGCCGC TCGTGGAAGA TGACACAGAG 
GAGCTGGAGG ACCCAGAGCT TGTGCTGGAC TGTGTCCCCA GGGAGGCTGA GCAGAAGGCC 
GGCCTGGGAA GCTCCTGTCC AGGAATAGAG CAGAGCAAGG ACAAGCAGAA GCAGAGGTTG 
ACTGCTCAGG AGAAAAGGCA GGCCTGCCTG CTGCGCAGGG ACTTGAGCCA CCTGGACCAG 
CTGCATGAAG AGAAAGACCT TTTCATCCAG AAGACAAGGG GGGAGCTACG TGCCTGCCGC 
AGGAGGATGG AACTCCTAGT CAAGCAGCAG GAGAGTGTGG CAGCCGAGAT AGCTGCAGAG 
AGGGCAGCCA ACAACATGGC AGCCATGGGC CGCCTGGAGG CAGCGTCGAG GCGCCTGTGC 
TCAGAGCTGA AGAACGAGCA GGACCTGCAG GCGCAGATGA TGGCGGTGCT GCAGCAGAGC 
CAGAGCGCCC TGTGGCAGAT TGAGGTCCAG GAGGGGCAGC TAGAGGACGC CCGCAAGTCG 
GCCCAAGAGG AGGCTGCAGC CGCCAGGAGA GGCCTTCAGG AGCGGGCAGC CAAGCAGCTG 
CACCGGGAGA TGGGGGCCTC GGGGAAGGCA GAGCGGAACA GGCTGCTGCG AGCCCGGAGA 
TGCGTGCACC TGCAGAAGGA GCTTGGACTC CGGCAGCAGA AGCTGGTGGA GGAAGCCCAG 
AAGAACCACC GGAAGGCCCT GAGGTTCCTG AAGGCCTCGT TGGGGAGGAT CCGTGAGCAG 
GAGCAGAAGG AGGAGCTGGA GACCCGAGAG CACATGCGCA GGCGCATGGA CGCGGTGCTG 
GCCCTGAAGA ACAGTATCTC CGCCAACCGG GAAACGCTCC GGAAATTTCA AGCATGGGGC 
CAGGCCAAGA CAGAGCTGGC AGAACAGAAG GCCCAGGCGG AGAAGAAGGC AATTCTGGCC 
CAAGGTGGGG ACGCCTTTAA GCACCTGTGC CATCAGCGCC GGCGCCAGGA GTTGGAGGCC 
CAGAACCGCG CCTTCGAGGA GGAGCAGAAG CGCAGGAAGC AGGAGATTGT GAGTCGGCTC 
TTGAAGGAAG AGGCTGTGGA GGACCGGAGG AGGCCACGGC CCGAGCCCCC GAGACCCGCA 
GGCCAGCGGA GCCTGAGGGA CCAGACCTGG AGCTACGTGG CCACCTTCTT CGATGGGCAG 
AGCACAGAGG CCCCGCTCTG CTGTCCCCTG GTGAGCGGAT GCCCTGGCGG CAACAGGACC 
ATGCCTGCCA GCCAAGATCA GCACCACGGA GAGCGCCCTG GCCCCAGAAC CTTTACTTCC 
TGCTTGCGTC TGATGCCCCA CCCCCACCAC CAGGGCCTTG GATGGCCTCA AGGCTCCCTC 
CCAAGGCAGG CGGGTGGCAG CCTCCCGGGG CTCTGGTGCC TCACCCACTA TCGTCTCGGG 
CCGCCTTACA TATGTGGGAC AGGGATCTTG GGACCCTGTC CTGAGCCCGG TGGCCCTTCC 
ACTTCTGCCC TGTGCATCCC CAACCTGAAG GCCCAGCTCA CTGGCTCTAA CAAGGGTGCT 
GCCGGCTGGG CCCCCAGGCT TCATTGCATG TTGCCCCCCA GGGTCTCCAC TCCTCTCAGT 
GCCCCTCTCC ACCCTGCTGT CCTGGGCAGC GCCCGCCTGC ACCTCCTGCT GCCTCCTCCC 
TATTGTCTCA CCAGCCAGAG TGCTCCTGCT CTTCTCCGCG GGGGGCCTTT CTCTCACCTG 
CAGCAACGTC AATCTCCACC CTTATCCCTA TTCCAGCCTG ATGTCCCTGG ACAGAGGCCA 
GGTCCAGCCC AAGAGCAGAT AAAGCTCCAG GAGACCAGGT CCAGATTCTA CCAGTCCTCT 
CCGCTGCTGA CGGCCATCTC CAGTGAGTCT GTCCAGGGGG ACGGGAGCCT TGGCGGGGAG 
GACACACTGG CTTGGCCGGA GATCCCCGGG CTCTGGAAGG AGGACTACAA GCCTTTCCAG 
GTATCCAAGG AGGATGTGGA CCGGAAGCCA GTGGGCGGAA CAAAGATGGA CAAGGACATC 
CTGGCGCGGA CAATGCAACG GCTACGCAGT GGGGTGATCC ACAAACAGGT GGCATCAGGG 
CGTGAGTTCA GAGGGTGCCC CTTCAACAGC AAGCCCAAGT TCATCCACTT CAAGGACTTT 
GATGTGGGCA AAGTGTACAA GAAGAAGATC ACGCTGATCA ACGCCACCTA CACGATTAAC 
TACTGCAAGC TGGTGGGCGT GGATGAGAGC CTGCAGGACT TCATCCACGT GGACTTTGAC 
CCCCCAGGCC CACTATCAGC CGGCATGTCC TGCGAAGTGC TTGTCACCTT CAAGCCCATG 
ATAAACAAAG ATCTGGAAGG AAATGTCTCG TTTCTGGCTC AGACAGGCGG ATTTTCTGTT 
CCACTGAAAT GTTCAACAAA GAAATGCTCT CTGTCCCTCG ACAAGGAGCT CATCGACTTT 
GGCAGCTACA CAGTGGGTGA GACTGCAATC CGGACCATCA CGCTGACCAA CACTGGGGGC 
TTGGGCACCA AGTTCAGATT TCTTCTGGCA TCAGAGACTT GTGAGATGGA CACCTCCCAG 
TCGGTCCTAA AATTAAGTAG TACCTTCACG TATGAAGACA AAAGTTTGTA TGATAAATTC 
ACCACCAGTT TTTCTGAGCA TCAGATGGAG GGCAATGAGT CATCTCCTAC AGATGTACCA 
AGCCAGAAGG AGTCTGGAAA GCTGGACGTG AAGGAGCAGG AGGAGGGGCA CCCCATCGAA 
TCAGAGGGCG TGACCTTGAC CACCATCATG ACTATACCTC TCAATGAGGA ACAGATGGAG 
TTCTCGCTGG GAGAGACAAC AGAGGGGGAC ATAGGCCCCT TCAGCTCCAT CAAGGTGCCC 
GTCATCTTCA CCCCGATTGT CCCGGGAACG GTCCAGAACA GTTTCAAGGT TGTGTTTAAG 
AATCAGCAGT GCCCAACTTT ATATTTCAAA GTCGTCGGCG TGGCTATTGA TGTGCCTGTC 
TGGGTGCCAA AGCCCAACGT AGACCTGAAG ATCTGCATGT ACGATCGGCT GTACCAGGAC 
TCGATCCTCG TCCACACGCG GTCGAAGGCC GCGTTGCGCC TAAAGTTCGA GGTGTGCAAG 
GAGCTGCGGG GCCACGTGGA GCTCCTGCCT GAGACTGGCT ACATCCAGGC CCTGTCGTCC 
TACTCAGTGC AGCTCAAGTT CCTGCCCAGA CACTCCCTCC CAGAAGATGC AGGGAAGTAT 
TTTGACAAGG AGACCAGGGT TCTGGAGGCC CCGATGACTA TCCGGGTGGC TGACCAGATC 
AAACCCGTGG GGTTTACTGT CCATGCTGTA GTCACCACCT CAGACCTGGA GCTCAGCCCC 
GCAGGGCTGG ACTTTGGCTA CTGTACCATC TACGAGGCCA TCAGGACCCA AATCAGCCTC 
TGCAATCATT CCCTCCTGCC CCAGGAGTTT GGGTTCGTTG GACTCCCCAA GTTTGTGGAC 
ATCCAGCCCA ACGATGGGTT CGGGACAATC CTGCCACTGG AAACGCTACA GCTCGATGTG 
ATCTTTCAGC CCACCAAGGC CAAGGAGTAC AACTTTGAGC TCATCTGCAA GTCAGAGATT 
AATCGGTGCT TCAAGCTGGC ATGCCGGGCC GTGGGTGTCT ACCCACCCCT GGAGCTGTCC 
CACAACCAGA TCAAGTTTGC AGCCACCTCC TTGTACGACA CATCCGTGGC CACTCTGTAC 
GTCATCAACT CCCACCTGAG CATGAACTCA CTGACCCACT CAGTGCCCCG CATTGGCTCA 
GAGGACGCTT TTCCCGTGGG GCCCACGTCT TTTGAGTTCC TGCTGCCCCC AGATTCGCCC 
ATCACCATCT CACCCCTGGT GGGCACCGTG TGGCCAGGAA AGAGGTGCCT GGTCCGAGTG 
GCCTTCCGGC CAGTGCTGCC CAGCGAGCTG ATCCACCAGG AGGCCTTCCA GGCCCTGAGC 
AGAAAAGCTG AGGTCAAATC GTCCCAAAAG GAGACGGTCA CCCAGAGGAA GGACCAGCGG 
AGACCATCTT TCTCCATGCT GCGGCTGCAG AACCAAGACC GGCTGCTCCG GGCCTCAGCC 
TCCCAGCCCC CGGAGCTGCA GAAGGAGGAC CTTGCATCCA GCTCTCAAGA GTACCAGTCG 
GCACAGGCCT CCCTGACCAG GTCTTTCCAG GGGAAGTTTG ACAAGTTTGT GGTTCCCTGT 
GTTGTTGCTA GCGGCGACAC CAAAGACAGG AAGGGGACAG AACCGCTGAG CTTCAGTCCC 
CACAACACGC TGTACCTGGA GCTGTGGTGT CCAGCAGTGG CGCCGTCTAT CGTGGTGACG 
TCCGACAAAG GCAAGACCAT CATTAACTTC GGCGACATTG CTGCAGGACA CCGGGGCATA 
AAGAAGGTCT CCATCCAGAA CATCTCCCCT GAGGATCTCG ACCTGGAGTA CTCCGTGCTG 
AACCCCGATG GCCCCTTCGT CCTGCTGAAC CCCACCATCA GGTTGCCCTC TGGCGAGACC 
CAGGTCCTAG ATCTGTCCTT CTCCCCACGT GAGAGTGTCG TGGCCCAAGA AATACTGGAC 
ATCATCACCA AGAAAGGCAC GCTCTCCCTG GCCCTCATGG GCACGGGTGT GGCGTCCACG 
ATCACGTGCT CCATTGAAGG TGATGTCCTC AACATGGGCT ACGTGATCGC CAGAGAGTCT 
GTCTCCTTGG GCTTCAAGCT GCAGAACAAC TCCCCGCTGC CCATCAAGTT CTCCATGCAG 
CTGGACAGCC TCTCCTCCAG GAGCAGAGAC CAGCACCGCC TGCCACAGTT CCTGGCCTCG 
CCTGACCAGA GGACGGAAGT CGTGGGCACG CAGAACTACA GTGGCCAGAG CGTGTTCAGC 
GTGGTCCCGG TCGAGGGCGT CATGGAGCCC GGCAAGGCTC AGGACTTCAC TGTGACCTTC 
AGCCCAGACC ACGAGAGCCT ATACTTCTCT GACCGGCTGC AGGTCGTGCT CTTTGAAAAG 
AAAGTGTCCC ACCACATCCT CCTGAAGGGC GCGGCCCGCG AGCACATGAT GTTCGTGGAG 
GGCGGAGACC CCCTGGACGT GCCCGTGGAG TCCCTGACCG TGATCCCTGC CTTTGACCTG 
GAGCACAGAG AGGGTGAACC CCCACCAGGC CCTCCTTCTC CAGAAGCTGA GGAGCTAAAG 
CCCATCCTGG TGACCCTGGA CTACGCCCAG CTGGACCCAG ACACACAGGC CCCACCTGCC 
ACCCGAGAAC TGCAGGTGGG CTGCATCCGG ACTACCCAGC TGTCCGCAAA GAAGACTGTT 
GAGTTCAGCA TGGACAACAT CGCAGCCCTG CAGCACAAGG GCTTCACCAT TGAGCCCTCC 
AGAGGCTCTG TGGAGCGCGG CCAGACCAAG GCCATCAGCA TCTCCTGGAC ACCACCCATC 
GACTTTGATC CGGACCACCC ACTCTTGGCA TCGGCCCTGC TGCAGCTCAG GGGGGATGTG 
AAGGAGACCT ACAAGGTCCT CTTTGTAGCC CAGGTGGTGA CCGGTCCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CFAP74 ( XM_015475230.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015330716.1 Bos taurus cilia and flagella associated protein 74 (CFAP74), mRNA.

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CloneID	OBa93026
Clone ID Related Accession (Same CDS sequence)	XM_015466809.1 , XM_015475230.1
Accession Version	XM_015475230.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5511bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	cilia- and flagella-associated protein 74
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104439.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

ATGGAAGAAG ACGACAAGAA GCTTTTGGAT GACAAGCCGC TCGTGGAAGA TGACACAGAG 
GAGCTGGAGG ACCCAGAGCT TGTGCTGGAC TGTGTCCCCA GGGAGGCTGA GCAGAAGGCC 
GGCCTGGGAA GCTCCTGTCC AGGAATAGAG CAGAGCAAGG ACAAGCAGAA GCAGAGGTTG 
ACTGCTCAGG AGAAAAGGCA GGCCTGCCTG CTGCGCAGGG ACTTGAGCCA CCTGGACCAG 
CTGCATGAAG AGAAAGACCT TTTCATCCAG AAGACAAGGG GGGAGCTACG TGCCTGCCGC 
AGGAGGATGG AACTCCTAGT CAAGCAGCAG GAGAGTGTGG CAGCCGAGAT AGCTGCAGAG 
AGGGCAGCCA ACAACATGGC AGCCATGGGC CGCCTGGAGG CAGCGTCGAG GCGCCTGTGC 
TCAGAGCTGA AGAACGAGCA GGACCTGCAG GCGCAGATGA TGGCGGTGCT GCAGCAGAGC 
CAGAGCGCCC TGTGGCAGAT TGAGGTCCAG GAGGGGCAGC TAGAGGACGC CCGCAAGTCG 
GCCCAAGAGG AGGCTGCAGC CGCCAGGAGA GGCCTTCAGG AGCGGGCAGC CAAGCAGCTG 
CACCGGGAGA TGGGGGCCTC GGGGAAGGCA GAGCGGAACA GGCTGCTGCG AGCCCGGAGA 
TGCGTGCACC TGCAGAAGGA GCTTGGACTC CGGCAGCAGA AGCTGGTGGA GGAAGCCCAG 
AAGAACCACC GGAAGGCCCT GAGGTTCCTG AAGGCCTCGT TGGGGAGGAT CCGTGAGCAG 
GAGCAGAAGG AGGAGCTGGA GACCCGAGAG CACATGCGCA GGCGCATGGA CGCGGTGCTG 
GCCCTGAAGA ACAGTATCTC CGCCAACCGG GAAACGCTCC GGAAATTTCA AGCATGGGGC 
CAGGCCAAGA CAGAGCTGGC AGAACAGAAG GCCCAGGCGG AGAAGAAGGC AATTCTGGCC 
CAAGGTGGGG ACGCCTTTAA GCACCTGTGC CATCAGCGCC GGCGCCAGGA GTTGGAGGCC 
CAGAACCGCG CCTTCGAGGA GGAGCAGAAG CGCAGGAAGC AGGAGATTGT GAGTCGGCTC 
TTGAAGGAAG AGGCTGTGGA GGACCGGAGG AGGCCACGGC CCGAGCCCCC GAGACCCGCA 
GGCCAGCGGA GCCTGAGGGA CCAGACCTGG AGCTACGTGG CCACCTTCTT CGATGGGCAG 
AGCACAGAGG CCCCGCTCTG CTGTCCCCTG GTGAGCGGAT GCCCTGGCGG CAACAGGACC 
ATGCCTGCCA GCCAAGATCA GCACCACGGA GAGCGCCCTG GCCCCAGAAC CTTTACTTCC 
TGCTTGCGTC TGATGCCCCA CCCCCACCAC CAGGGCCTTG GATGGCCTCA AGGCTCCCTC 
CCAAGGCAGG CGGGTGGCAG CCTCCCGGGG CTCTGGTGCC TCACCCACTA TCGTCTCGGG 
CCGCCTTACA TATGTGGGAC AGGGATCTTG GGACCCTGTC CTGAGCCCGG TGGCCCTTCC 
ACTTCTGCCC TGTGCATCCC CAACCTGAAG GCCCAGCTCA CTGGCTCTAA CAAGGGTGCT 
GCCGGCTGGG CCCCCAGGCT TCATTGCATG TTGCCCCCCA GGGTCTCCAC TCCTCTCAGT 
GCCCCTCTCC ACCCTGCTGT CCTGGGCAGC GCCCGCCTGC ACCTCCTGCT GCCTCCTCCC 
TATTGTCTCA CCAGCCAGAG TGCTCCTGCT CTTCTCCGCG GGGGGCCTTT CTCTCACCTG 
CAGCAACGTC AATCTCCACC CTTATCCCTA TTCCAGCCTG ATGTCCCTGG ACAGAGGCCA 
GGTCCAGCCC AAGAGCAGAT AAAGCTCCAG GAGACCAGGT CCAGATTCTA CCAGTCCTCT 
CCGCTGCTGA CGGCCATCTC CAGTGAGTCT GTCCAGGGGG ACGGGAGCCT TGGCGGGGAG 
GACACACTGG CTTGGCCGGA GATCCCCGGG CTCTGGAAGG AGGACTACAA GCCTTTCCAG 
GTATCCAAGG AGGATGTGGA CCGGAAGCCA GTGGGCGGAA CAAAGATGGA CAAGGACATC 
CTGGCGCGGA CAATGCAACG GCTACGCAGT GGGGTGATCC ACAAACAGGT GGCATCAGGG 
CGTGAGTTCA GAGGGTGCCC CTTCAACAGC AAGCCCAAGT TCATCCACTT CAAGGACTTT 
GATGTGGGCA AAGTGTACAA GAAGAAGATC ACGCTGATCA ACGCCACCTA CACGATTAAC 
TACTGCAAGC TGGTGGGCGT GGATGAGAGC CTGCAGGACT TCATCCACGT GGACTTTGAC 
CCCCCAGGCC CACTATCAGC CGGCATGTCC TGCGAAGTGC TTGTCACCTT CAAGCCCATG 
ATAAACAAAG ATCTGGAAGG AAATGTCTCG TTTCTGGCTC AGACAGGCGG ATTTTCTGTT 
CCACTGAAAT GTTCAACAAA GAAATGCTCT CTGTCCCTCG ACAAGGAGCT CATCGACTTT 
GGCAGCTACA CAGTGGGTGA GACTGCAATC CGGACCATCA CGCTGACCAA CACTGGGGGC 
TTGGGCACCA AGTTCAGATT TCTTCTGGCA TCAGAGACTT GTGAGATGGA CACCTCCCAG 
TCGGTCCTAA AATTAAGTAG TACCTTCACG TATGAAGACA AAAGTTTGTA TGATAAATTC 
ACCACCAGTT TTTCTGAGCA TCAGATGGAG GGCAATGAGT CATCTCCTAC AGATGTACCA 
AGCCAGAAGG AGTCTGGAAA GCTGGACGTG AAGGAGCAGG AGGAGGGGCA CCCCATCGAA 
TCAGAGGGCG TGACCTTGAC CACCATCATG ACTATACCTC TCAATGAGGA ACAGATGGAG 
TTCTCGCTGG GAGAGACAAC AGAGGGGGAC ATAGGCCCCT TCAGCTCCAT CAAGGTGCCC 
GTCATCTTCA CCCCGATTGT CCCGGGAACG GTCCAGAACA GTTTCAAGGT TGTGTTTAAG 
AATCAGCAGT GCCCAACTTT ATATTTCAAA GTCGTCGGCG TGGCTATTGA TGTGCCTGTC 
TGGGTGCCAA AGCCCAACGT AGACCTGAAG ATCTGCATGT ACGATCGGCT GTACCAGGAC 
TCGATCCTCG TCCACACGCG GTCGAAGGCC GCGTTGCGCC TAAAGTTCGA GGTGTGCAAG 
GAGCTGCGGG GCCACGTGGA GCTCCTGCCT GAGACTGGCT ACATCCAGGC CCTGTCGTCC 
TACTCAGTGC AGCTCAAGTT CCTGCCCAGA CACTCCCTCC CAGAAGATGC AGGGAAGTAT 
TTTGACAAGG AGACCAGGGT TCTGGAGGCC CCGATGACTA TCCGGGTGGC TGACCAGATC 
AAACCCGTGG GGTTTACTGT CCATGCTGTA GTCACCACCT CAGACCTGGA GCTCAGCCCC 
GCAGGGCTGG ACTTTGGCTA CTGTACCATC TACGAGGCCA TCAGGACCCA AATCAGCCTC 
TGCAATCATT CCCTCCTGCC CCAGGAGTTT GGGTTCGTTG GACTCCCCAA GTTTGTGGAC 
ATCCAGCCCA ACGATGGGTT CGGGACAATC CTGCCACTGG AAACGCTACA GCTCGATGTG 
ATCTTTCAGC CCACCAAGGC CAAGGAGTAC AACTTTGAGC TCATCTGCAA GTCAGAGATT 
AATCGGTGCT TCAAGCTGGC ATGCCGGGCC GTGGGTGTCT ACCCACCCCT GGAGCTGTCC 
CACAACCAGA TCAAGTTTGC AGCCACCTCC TTGTACGACA CATCCGTGGC CACTCTGTAC 
GTCATCAACT CCCACCTGAG CATGAACTCA CTGACCCACT CAGTGCCCCG CATTGGCTCA 
GAGGACGCTT TTCCCGTGGG GCCCACGTCT TTTGAGTTCC TGCTGCCCCC AGATTCGCCC 
ATCACCATCT CACCCCTGGT GGGCACCGTG TGGCCAGGAA AGAGGTGCCT GGTCCGAGTG 
GCCTTCCGGC CAGTGCTGCC CAGCGAGCTG ATCCACCAGG AGGCCTTCCA GGCCCTGAGC 
AGAAAAGCTG AGGTCAAATC GTCCCAAAAG GAGACGGTCA CCCAGAGGAA GGACCAGCGG 
AGACCATCTT TCTCCATGCT GCGGCTGCAG AACCAAGACC GGCTGCTCCG GGCCTCAGCC 
TCCCAGCCCC CGGAGCTGCA GAAGGAGGAC CTTGCATCCA GCTCTCAAGA GTACCAGTCG 
GCACAGGCCT CCCTGACCAG GTCTTTCCAG GGGAAGTTTG ACAAGTTTGT GGTTCCCTGT 
GTTGTTGCTA GCGGCGACAC CAAAGACAGG AAGGGGACAG AACCGCTGAG CTTCAGTCCC 
CACAACACGC TGTACCTGGA GCTGTGGTGT CCAGCAGTGG CGCCGTCTAT CGTGGTGACG 
TCCGACAAAG GCAAGACCAT CATTAACTTC GGCGACATTG CTGCAGGACA CCGGGGCATA 
AAGAAGGTCT CCATCCAGAA CATCTCCCCT GAGGATCTCG ACCTGGAGTA CTCCGTGCTG 
AACCCCGATG GCCCCTTCGT CCTGCTGAAC CCCACCATCA GGTTGCCCTC TGGCGAGACC 
CAGGTCCTAG ATCTGTCCTT CTCCCCACGT GAGAGTGTCG TGGCCCAAGA AATACTGGAC 
ATCATCACCA AGAAAGGCAC GCTCTCCCTG GCCCTCATGG GCACGGGTGT GGCGTCCACG 
ATCACGTGCT CCATTGAAGG TGATGTCCTC AACATGGGCT ACGTGATCGC CAGAGAGTCT 
GTCTCCTTGG GCTTCAAGCT GCAGAACAAC TCCCCGCTGC CCATCAAGTT CTCCATGCAG 
CTGGACAGCC TCTCCTCCAG GAGCAGAGAC CAGCACCGCC TGCCACAGTT CCTGGCCTCG 
CCTGACCAGA GGACGGAAGT CGTGGGCACG CAGAACTACA GTGGCCAGAG CGTGTTCAGC 
GTGGTCCCGG TCGAGGGCGT CATGGAGCCC GGCAAGGCTC AGGACTTCAC TGTGACCTTC 
AGCCCAGACC ACGAGAGCCT ATACTTCTCT GACCGGCTGC AGGTCGTGCT CTTTGAAAAG 
AAAGTGTCCC ACCACATCCT CCTGAAGGGC GCGGCCCGCG AGCACATGAT GTTCGTGGAG 
GGCGGAGACC CCCTGGACGT GCCCGTGGAG TCCCTGACCG TGATCCCTGC CTTTGACCTG 
GAGCACAGAG AGGGTGAACC CCCACCAGGC CCTCCTTCTC CAGAAGCTGA GGAGCTAAAG 
CCCATCCTGG TGACCCTGGA CTACGCCCAG CTGGACCCAG ACACACAGGC CCCACCTGCC 
ACCCGAGAAC TGCAGGTGGG CTGCATCCGG ACTACCCAGC TGTCCGCAAA GAAGACTGTT 
GAGTTCAGCA TGGACAACAT CGCAGCCCTG CAGCACAAGG GCTTCACCAT TGAGCCCTCC 
AGAGGCTCTG TGGAGCGCGG CCAGACCAAG GCCATCAGCA TCTCCTGGAC ACCACCCATC 
GACTTTGATC CGGACCACCC ACTCTTGGCA TCGGCCCTGC TGCAGCTCAG GGGGGATGTG 
AAGGAGACCT ACAAGGTCCT CTTTGTAGCC CAGGTGGTGA CCGGTCCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015330716.1
CDS	398..5908
Translation	MEEDDKKLLDDKPLVEDDTEELEDPELVLDCVPREAEQKAGLGSSCPGIEQSKDKQKQRLTAQEKRQACLLRRDLSHLDQLHEEKDLFIQKTRGELRACRRRMELLVKQQESVAAEIAAERAANNMAAMGRLEAASRRLCSELKNEQDLQAQMMAVLQQSQSALWQIEVQEGQLEDARKSAQEEAAAARRGLQERAAKQLHREMGASGKAERNRLLRARRCVHLQKELGLRQQKLVEEAQKNHRKALRFLKASLGRIREQEQKEELETREHMRRRMDAVLALKNSISANRETLRKFQAWGQAKTELAEQKAQAEKKAILAQGGDAFKHLCHQRRRQELEAQNRAFEEEQKRRKQEIVSRLLKEEAVEDRRRPRPEPPRPAGQRSLRDQTWSYVATFFDGQSTEAPLCCPLVSGCPGGNRTMPASQDQHHGERPGPRTFTSCLRLMPHPHHQGLGWPQGSLPRQAGGSLPGLWCLTHYRLGPPYICGTGILGPCPEPGGPSTSALCIPNLKAQLTGSNKGAAGWAPRLHCMLPPRVSTPLSAPLHPAVLGSARLHLLLPPPYCLTSQSAPALLRGGPFSHLQQRQSPPLSLFQPDVPGQRPGPAQEQIKLQETRSRFYQSSPLLTAISSESVQGDGSLGGEDTLAWPEIPGLWKEDYKPFQVSKEDVDRKPVGGTKMDKDILARTMQRLRSGVIHKQVASGREFRGCPFNSKPKFIHFKDFDVGKVYKKKITLINATYTINYCKLVGVDESLQDFIHVDFDPPGPLSAGMSCEVLVTFKPMINKDLEGNVSFLAQTGGFSVPLKCSTKKCSLSLDKELIDFGSYTVGETAIRTITLTNTGGLGTKFRFLLASETCEMDTSQSVLKLSSTFTYEDKSLYDKFTTSFSEHQMEGNESSPTDVPSQKESGKLDVKEQEEGHPIESEGVTLTTIMTIPLNEEQMEFSLGETTEGDIGPFSSIKVPVIFTPIVPGTVQNSFKVVFKNQQCPTLYFKVVGVAIDVPVWVPKPNVDLKICMYDRLYQDSILVHTRSKAALRLKFEVCKELRGHVELLPETGYIQALSSYSVQLKFLPRHSLPEDAGKYFDKETRVLEAPMTIRVADQIKPVGFTVHAVVTTSDLELSPAGLDFGYCTIYEAIRTQISLCNHSLLPQEFGFVGLPKFVDIQPNDGFGTILPLETLQLDVIFQPTKAKEYNFELICKSEINRCFKLACRAVGVYPPLELSHNQIKFAATSLYDTSVATLYVINSHLSMNSLTHSVPRIGSEDAFPVGPTSFEFLLPPDSPITISPLVGTVWPGKRCLVRVAFRPVLPSELIHQEAFQALSRKAEVKSSQKETVTQRKDQRRPSFSMLRLQNQDRLLRASASQPPELQKEDLASSSQEYQSAQASLTRSFQGKFDKFVVPCVVASGDTKDRKGTEPLSFSPHNTLYLELWCPAVAPSIVVTSDKGKTIINFGDIAAGHRGIKKVSIQNISPEDLDLEYSVLNPDGPFVLLNPTIRLPSGETQVLDLSFSPRESVVAQEILDIITKKGTLSLALMGTGVASTITCSIEGDVLNMGYVIARESVSLGFKLQNNSPLPIKFSMQLDSLSSRSRDQHRLPQFLASPDQRTEVVGTQNYSGQSVFSVVPVEGVMEPGKAQDFTVTFSPDHESLYFSDRLQVVLFEKKVSHHILLKGAAREHMMFVEGGDPLDVPVESLTVIPAFDLEHREGEPPPGPPSPEAEELKPILVTLDYAQLDPDTQAPPATRELQVGCIRTTQLSAKKTVEFSMDNIAALQHKGFTIEPSRGSVERGQTKAISISWTPPIDFDPDHPLLASALLQLRGDVKETYKVLFVAQVVTGP

Target ORF information:

RefSeq Version	XM_015475230.1
Organism	Bos taurus(cattle)
Definition	Bos taurus cilia and flagella associated protein 74 (CFAP74), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015475230.1

ORF Insert Sequence:

ATGGAAGAAG ACGACAAGAA GCTTTTGGAT GACAAGCCGC TCGTGGAAGA TGACACAGAG 
GAGCTGGAGG ACCCAGAGCT TGTGCTGGAC TGTGTCCCCA GGGAGGCTGA GCAGAAGGCC 
GGCCTGGGAA GCTCCTGTCC AGGAATAGAG CAGAGCAAGG ACAAGCAGAA GCAGAGGTTG 
ACTGCTCAGG AGAAAAGGCA GGCCTGCCTG CTGCGCAGGG ACTTGAGCCA CCTGGACCAG 
CTGCATGAAG AGAAAGACCT TTTCATCCAG AAGACAAGGG GGGAGCTACG TGCCTGCCGC 
AGGAGGATGG AACTCCTAGT CAAGCAGCAG GAGAGTGTGG CAGCCGAGAT AGCTGCAGAG 
AGGGCAGCCA ACAACATGGC AGCCATGGGC CGCCTGGAGG CAGCGTCGAG GCGCCTGTGC 
TCAGAGCTGA AGAACGAGCA GGACCTGCAG GCGCAGATGA TGGCGGTGCT GCAGCAGAGC 
CAGAGCGCCC TGTGGCAGAT TGAGGTCCAG GAGGGGCAGC TAGAGGACGC CCGCAAGTCG 
GCCCAAGAGG AGGCTGCAGC CGCCAGGAGA GGCCTTCAGG AGCGGGCAGC CAAGCAGCTG 
CACCGGGAGA TGGGGGCCTC GGGGAAGGCA GAGCGGAACA GGCTGCTGCG AGCCCGGAGA 
TGCGTGCACC TGCAGAAGGA GCTTGGACTC CGGCAGCAGA AGCTGGTGGA GGAAGCCCAG 
AAGAACCACC GGAAGGCCCT GAGGTTCCTG AAGGCCTCGT TGGGGAGGAT CCGTGAGCAG 
GAGCAGAAGG AGGAGCTGGA GACCCGAGAG CACATGCGCA GGCGCATGGA CGCGGTGCTG 
GCCCTGAAGA ACAGTATCTC CGCCAACCGG GAAACGCTCC GGAAATTTCA AGCATGGGGC 
CAGGCCAAGA CAGAGCTGGC AGAACAGAAG GCCCAGGCGG AGAAGAAGGC AATTCTGGCC 
CAAGGTGGGG ACGCCTTTAA GCACCTGTGC CATCAGCGCC GGCGCCAGGA GTTGGAGGCC 
CAGAACCGCG CCTTCGAGGA GGAGCAGAAG CGCAGGAAGC AGGAGATTGT GAGTCGGCTC 
TTGAAGGAAG AGGCTGTGGA GGACCGGAGG AGGCCACGGC CCGAGCCCCC GAGACCCGCA 
GGCCAGCGGA GCCTGAGGGA CCAGACCTGG AGCTACGTGG CCACCTTCTT CGATGGGCAG 
AGCACAGAGG CCCCGCTCTG CTGTCCCCTG GTGAGCGGAT GCCCTGGCGG CAACAGGACC 
ATGCCTGCCA GCCAAGATCA GCACCACGGA GAGCGCCCTG GCCCCAGAAC CTTTACTTCC 
TGCTTGCGTC TGATGCCCCA CCCCCACCAC CAGGGCCTTG GATGGCCTCA AGGCTCCCTC 
CCAAGGCAGG CGGGTGGCAG CCTCCCGGGG CTCTGGTGCC TCACCCACTA TCGTCTCGGG 
CCGCCTTACA TATGTGGGAC AGGGATCTTG GGACCCTGTC CTGAGCCCGG TGGCCCTTCC 
ACTTCTGCCC TGTGCATCCC CAACCTGAAG GCCCAGCTCA CTGGCTCTAA CAAGGGTGCT 
GCCGGCTGGG CCCCCAGGCT TCATTGCATG TTGCCCCCCA GGGTCTCCAC TCCTCTCAGT 
GCCCCTCTCC ACCCTGCTGT CCTGGGCAGC GCCCGCCTGC ACCTCCTGCT GCCTCCTCCC 
TATTGTCTCA CCAGCCAGAG TGCTCCTGCT CTTCTCCGCG GGGGGCCTTT CTCTCACCTG 
CAGCAACGTC AATCTCCACC CTTATCCCTA TTCCAGCCTG ATGTCCCTGG ACAGAGGCCA 
GGTCCAGCCC AAGAGCAGAT AAAGCTCCAG GAGACCAGGT CCAGATTCTA CCAGTCCTCT 
CCGCTGCTGA CGGCCATCTC CAGTGAGTCT GTCCAGGGGG ACGGGAGCCT TGGCGGGGAG 
GACACACTGG CTTGGCCGGA GATCCCCGGG CTCTGGAAGG AGGACTACAA GCCTTTCCAG 
GTATCCAAGG AGGATGTGGA CCGGAAGCCA GTGGGCGGAA CAAAGATGGA CAAGGACATC 
CTGGCGCGGA CAATGCAACG GCTACGCAGT GGGGTGATCC ACAAACAGGT GGCATCAGGG 
CGTGAGTTCA GAGGGTGCCC CTTCAACAGC AAGCCCAAGT TCATCCACTT CAAGGACTTT 
GATGTGGGCA AAGTGTACAA GAAGAAGATC ACGCTGATCA ACGCCACCTA CACGATTAAC 
TACTGCAAGC TGGTGGGCGT GGATGAGAGC CTGCAGGACT TCATCCACGT GGACTTTGAC 
CCCCCAGGCC CACTATCAGC CGGCATGTCC TGCGAAGTGC TTGTCACCTT CAAGCCCATG 
ATAAACAAAG ATCTGGAAGG AAATGTCTCG TTTCTGGCTC AGACAGGCGG ATTTTCTGTT 
CCACTGAAAT GTTCAACAAA GAAATGCTCT CTGTCCCTCG ACAAGGAGCT CATCGACTTT 
GGCAGCTACA CAGTGGGTGA GACTGCAATC CGGACCATCA CGCTGACCAA CACTGGGGGC 
TTGGGCACCA AGTTCAGATT TCTTCTGGCA TCAGAGACTT GTGAGATGGA CACCTCCCAG 
TCGGTCCTAA AATTAAGTAG TACCTTCACG TATGAAGACA AAAGTTTGTA TGATAAATTC 
ACCACCAGTT TTTCTGAGCA TCAGATGGAG GGCAATGAGT CATCTCCTAC AGATGTACCA 
AGCCAGAAGG AGTCTGGAAA GCTGGACGTG AAGGAGCAGG AGGAGGGGCA CCCCATCGAA 
TCAGAGGGCG TGACCTTGAC CACCATCATG ACTATACCTC TCAATGAGGA ACAGATGGAG 
TTCTCGCTGG GAGAGACAAC AGAGGGGGAC ATAGGCCCCT TCAGCTCCAT CAAGGTGCCC 
GTCATCTTCA CCCCGATTGT CCCGGGAACG GTCCAGAACA GTTTCAAGGT TGTGTTTAAG 
AATCAGCAGT GCCCAACTTT ATATTTCAAA GTCGTCGGCG TGGCTATTGA TGTGCCTGTC 
TGGGTGCCAA AGCCCAACGT AGACCTGAAG ATCTGCATGT ACGATCGGCT GTACCAGGAC 
TCGATCCTCG TCCACACGCG GTCGAAGGCC GCGTTGCGCC TAAAGTTCGA GGTGTGCAAG 
GAGCTGCGGG GCCACGTGGA GCTCCTGCCT GAGACTGGCT ACATCCAGGC CCTGTCGTCC 
TACTCAGTGC AGCTCAAGTT CCTGCCCAGA CACTCCCTCC CAGAAGATGC AGGGAAGTAT 
TTTGACAAGG AGACCAGGGT TCTGGAGGCC CCGATGACTA TCCGGGTGGC TGACCAGATC 
AAACCCGTGG GGTTTACTGT CCATGCTGTA GTCACCACCT CAGACCTGGA GCTCAGCCCC 
GCAGGGCTGG ACTTTGGCTA CTGTACCATC TACGAGGCCA TCAGGACCCA AATCAGCCTC 
TGCAATCATT CCCTCCTGCC CCAGGAGTTT GGGTTCGTTG GACTCCCCAA GTTTGTGGAC 
ATCCAGCCCA ACGATGGGTT CGGGACAATC CTGCCACTGG AAACGCTACA GCTCGATGTG 
ATCTTTCAGC CCACCAAGGC CAAGGAGTAC AACTTTGAGC TCATCTGCAA GTCAGAGATT 
AATCGGTGCT TCAAGCTGGC ATGCCGGGCC GTGGGTGTCT ACCCACCCCT GGAGCTGTCC 
CACAACCAGA TCAAGTTTGC AGCCACCTCC TTGTACGACA CATCCGTGGC CACTCTGTAC 
GTCATCAACT CCCACCTGAG CATGAACTCA CTGACCCACT CAGTGCCCCG CATTGGCTCA 
GAGGACGCTT TTCCCGTGGG GCCCACGTCT TTTGAGTTCC TGCTGCCCCC AGATTCGCCC 
ATCACCATCT CACCCCTGGT GGGCACCGTG TGGCCAGGAA AGAGGTGCCT GGTCCGAGTG 
GCCTTCCGGC CAGTGCTGCC CAGCGAGCTG ATCCACCAGG AGGCCTTCCA GGCCCTGAGC 
AGAAAAGCTG AGGTCAAATC GTCCCAAAAG GAGACGGTCA CCCAGAGGAA GGACCAGCGG 
AGACCATCTT TCTCCATGCT GCGGCTGCAG AACCAAGACC GGCTGCTCCG GGCCTCAGCC 
TCCCAGCCCC CGGAGCTGCA GAAGGAGGAC CTTGCATCCA GCTCTCAAGA GTACCAGTCG 
GCACAGGCCT CCCTGACCAG GTCTTTCCAG GGGAAGTTTG ACAAGTTTGT GGTTCCCTGT 
GTTGTTGCTA GCGGCGACAC CAAAGACAGG AAGGGGACAG AACCGCTGAG CTTCAGTCCC 
CACAACACGC TGTACCTGGA GCTGTGGTGT CCAGCAGTGG CGCCGTCTAT CGTGGTGACG 
TCCGACAAAG GCAAGACCAT CATTAACTTC GGCGACATTG CTGCAGGACA CCGGGGCATA 
AAGAAGGTCT CCATCCAGAA CATCTCCCCT GAGGATCTCG ACCTGGAGTA CTCCGTGCTG 
AACCCCGATG GCCCCTTCGT CCTGCTGAAC CCCACCATCA GGTTGCCCTC TGGCGAGACC 
CAGGTCCTAG ATCTGTCCTT CTCCCCACGT GAGAGTGTCG TGGCCCAAGA AATACTGGAC 
ATCATCACCA AGAAAGGCAC GCTCTCCCTG GCCCTCATGG GCACGGGTGT GGCGTCCACG 
ATCACGTGCT CCATTGAAGG TGATGTCCTC AACATGGGCT ACGTGATCGC CAGAGAGTCT 
GTCTCCTTGG GCTTCAAGCT GCAGAACAAC TCCCCGCTGC CCATCAAGTT CTCCATGCAG 
CTGGACAGCC TCTCCTCCAG GAGCAGAGAC CAGCACCGCC TGCCACAGTT CCTGGCCTCG 
CCTGACCAGA GGACGGAAGT CGTGGGCACG CAGAACTACA GTGGCCAGAG CGTGTTCAGC 
GTGGTCCCGG TCGAGGGCGT CATGGAGCCC GGCAAGGCTC AGGACTTCAC TGTGACCTTC 
AGCCCAGACC ACGAGAGCCT ATACTTCTCT GACCGGCTGC AGGTCGTGCT CTTTGAAAAG 
AAAGTGTCCC ACCACATCCT CCTGAAGGGC GCGGCCCGCG AGCACATGAT GTTCGTGGAG 
GGCGGAGACC CCCTGGACGT GCCCGTGGAG TCCCTGACCG TGATCCCTGC CTTTGACCTG 
GAGCACAGAG AGGGTGAACC CCCACCAGGC CCTCCTTCTC CAGAAGCTGA GGAGCTAAAG 
CCCATCCTGG TGACCCTGGA CTACGCCCAG CTGGACCCAG ACACACAGGC CCCACCTGCC 
ACCCGAGAAC TGCAGGTGGG CTGCATCCGG ACTACCCAGC TGTCCGCAAA GAAGACTGTT 
GAGTTCAGCA TGGACAACAT CGCAGCCCTG CAGCACAAGG GCTTCACCAT TGAGCCCTCC 
AGAGGCTCTG TGGAGCGCGG CCAGACCAAG GCCATCAGCA TCTCCTGGAC ACCACCCATC 
GACTTTGATC CGGACCACCC ACTCTTGGCA TCGGCCCTGC TGCAGCTCAG GGGGGATGTG 
AAGGAGACCT ACAAGGTCCT CTTTGTAGCC CAGGTGGTGA CCGGTCCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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CFAP74 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Eメール

電話

FAX

オンラインフォーム

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