MID2 gene cDNA ORF clone, Bos taurus(cattle)

mRNA	Protein	Name
XM_024988376.1	XP_024844144.1	probable E3 ubiquitin-protein ligase MID2 isoform X3
XM_010821742.2	XP_010820044.1	probable E3 ubiquitin-protein ligase MID2 isoform X2
XM_015470356.1	XP_015325842.1	probable E3 ubiquitin-protein ligase MID2 isoform X1
XM_024988373.1	XP_024844141.1	probable E3 ubiquitin-protein ligase MID2 isoform X1
XM_015461593.1	XP_015317079.1	probable E3 ubiquitin-protein ligase MID2 isoform X1
XM_010821742.3	XP_010820044.2	probable E3 ubiquitin-protein ligase MID2 isoform X2
XM_010799702.2	XP_010798004.1	probable E3 ubiquitin-protein ligase MID2 isoform X2
XM_024988374.1	XP_024844142.1	probable E3 ubiquitin-protein ligase MID2 isoform X3
XM_024988375.1	XP_024844143.1	probable E3 ubiquitin-protein ligase MID2 isoform X3

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa56147	XM_010799702.2 Latest version!	Bos taurus midline 2 (MID2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OBa56147	XM_010821742.2 Latest version!	Bos taurus midline 2 (MID2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OBa89317	XM_015470356.1 Latest version!	Bos taurus midline 2 (MID2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00
OBa89317	XM_015461593.1 Latest version!	Bos taurus midline 2 (MID2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00
OBa238550	XM_024988373.1 Latest version!	Bos taurus midline 2 (MID2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OBa238551	XM_010821742.3 Latest version!	Bos taurus midline 2 (MID2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OBa238552	XM_024988375.1 Latest version!	Bos taurus midline 2 (MID2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OBa238552	XM_024988374.1 Latest version!	Bos taurus midline 2 (MID2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OBa238552	XM_024988376.1 Latest version!	Bos taurus midline 2 (MID2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

MID2 ( XM_010821742.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010820044.1 Bos taurus midline 2 (MID2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa56147
Clone ID Related Accession (Same CDS sequence)	XM_010821742.2 , XM_010799702.2
Accession Version	XM_010821742.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1386bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	probable E3 ubiquitin-protein ligase MID2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104715.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010821742.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACCTCA CCAACCTGGT TAAGCGCAAC AGTGAACTAG AAAATCAAAT GGCCAAGCTA 
ATACAGATCT GCCAGCAGGT TGAGGTGAAT ACCGCTATGC ATGAGGCAAA ACTTATGGAA 
GAATGTGACG AATTGGTAGA GATCATCCAG CAGAGGAAGC AAATGATTGC TGTCAAAATC 
AAAGAGACAA AGGTTATGAA ACTGAGAAAA TTGGCACAGC AGGTTGCTAA TTGCCGCCAG 
TGTCTTGAAC GGTCAACAGT CCTCATCAAC CAAGCTGAGC ATATCCTGAA AGAAAATGAC 
CAGGCACGGT TTCTCCAGTC TGCAAAAAAT ATCGCTGAGA GGGTCGCTAT GGCAACTGCA 
TCTTCTCAAG TTCTGATTCC AGACATCAAT TTTAATGATG CCTTTGAAAA CTTTGCTTTA 
GATTTTTCCA GAGAAAAGAA ACTGCTGGAG GGGCTAGATT ATTTAACAGC CCCCAACCCA 
CCATCTATCC GAGAGGAACT CTGTACTGCC TCCCATGACA CCATTACGGT CCACTGGATC 
TCGGATGATG AGTTCAGCAT CAGCTCCTAT GAGCTTCAGT ACACCATATT CACTGGCCAG 
GCTAACTTCA TCAGCCTGTA TAATTCTGTG GATAGCTGGA TGATCGTGCC CAACATCAAA 
CAGAACCATT ACACAGTCCA CGGACTGCAG AGTGGAACAC GCTACATCTT CATTGTTAAA 
GCCATAAACC AAGCAGGCAG CCGGAACAGT GAACCTACCC GACTCAAAAC AAACAGCCAA 
CCCTTTAAAC TGGATCCCAA AATGACTCAC AAGAAGTTGA AGATTTCCAA TGATGGATTG 
CAGATGGAGA AGGAAGAAAG CTCTCTGAAG AAGAGCCATA CTCCAGAGAG ATTTAGTGGC 
ACTGGGTGCT ATGGGGCAGC AGGAAATATA TTCATTGACA GCGGTTGCCA CTACTGGGAG 
GTGGTCATGG GGTCCTCCAC ATGGTATGCA GTTGGCGTTG CCTATAAATC AGCTCCCAAG 
AATGAATGGA TTGGCAAGAA CGCCTCCTCG TGGGTCTTCT CGCGATGCAA TAGTAACTTC 
GTAGTGAGAC ATAACAACAA GGAAATGCTG GTGGACGTGC CTCCACAGTT GAAGCGCCTG 
GGTGTCCTTC TGGATTATGA CAACAACATG CTGTCTTTCT ATGACCCAGC TAACTCTCTC 
CATCTTCATA CTTTTGATGT GACCTTCATT CTTCCCGTTT GTCCAACATT CACAATTTGG 
AACAAATCCC TAATGATCCT GTCTGGCTTG CCTGCCCCAG ATTTTATTGA TTACCCTGAG 
CGGCAGGAAT GCAACTGCAG GCCTCAAGAA TCCCCTTATG TTTCTGGGAT GAAAGCTTGC 
CATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010820044.1
CDS	2055..3440
Translation	MNLTNLVKRNSELENQMAKLIQICQQVEVNTAMHEAKLMEECDELVEIIQQRKQMIAVKIKETKVMKLRKLAQQVANCRQCLERSTVLINQAEHILKENDQARFLQSAKNIAERVAMATASSQVLIPDINFNDAFENFALDFSREKKLLEGLDYLTAPNPPSIREELCTASHDTITVHWISDDEFSISSYELQYTIFTGQANFISLYNSVDSWMIVPNIKQNHYTVHGLQSGTRYIFIVKAINQAGSRNSEPTRLKTNSQPFKLDPKMTHKKLKISNDGLQMEKEESSLKKSHTPERFSGTGCYGAAGNIFIDSGCHYWEVVMGSSTWYAVGVAYKSAPKNEWIGKNASSWVFSRCNSNFVVRHNNKEMLVDVPPQLKRLGVLLDYDNNMLSFYDPANSLHLHTFDVTFILPVCPTFTIWNKSLMILSGLPAPDFIDYPERQECNCRPQESPYVSGMKACH

Target ORF information:

RefSeq Version	XM_010821742.2
Organism	Bos taurus(cattle)
Definition	Bos taurus midline 2 (MID2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010821742.2

ORF Insert Sequence:

ATGAACCTCA CCAACCTGGT TAAGCGCAAC AGTGAACTAG AAAATCAAAT GGCCAAGCTA 
ATACAGATCT GCCAGCAGGT TGAGGTGAAT ACCGCTATGC ATGAGGCAAA ACTTATGGAA 
GAATGTGACG AATTGGTAGA GATCATCCAG CAGAGGAAGC AAATGATTGC TGTCAAAATC 
AAAGAGACAA AGGTTATGAA ACTGAGAAAA TTGGCACAGC AGGTTGCTAA TTGCCGCCAG 
TGTCTTGAAC GGTCAACAGT CCTCATCAAC CAAGCTGAGC ATATCCTGAA AGAAAATGAC 
CAGGCACGGT TTCTCCAGTC TGCAAAAAAT ATCGCTGAGA GGGTCGCTAT GGCAACTGCA 
TCTTCTCAAG TTCTGATTCC AGACATCAAT TTTAATGATG CCTTTGAAAA CTTTGCTTTA 
GATTTTTCCA GAGAAAAGAA ACTGCTGGAG GGGCTAGATT ATTTAACAGC CCCCAACCCA 
CCATCTATCC GAGAGGAACT CTGTACTGCC TCCCATGACA CCATTACGGT CCACTGGATC 
TCGGATGATG AGTTCAGCAT CAGCTCCTAT GAGCTTCAGT ACACCATATT CACTGGCCAG 
GCTAACTTCA TCAGCCTGTA TAATTCTGTG GATAGCTGGA TGATCGTGCC CAACATCAAA 
CAGAACCATT ACACAGTCCA CGGACTGCAG AGTGGAACAC GCTACATCTT CATTGTTAAA 
GCCATAAACC AAGCAGGCAG CCGGAACAGT GAACCTACCC GACTCAAAAC AAACAGCCAA 
CCCTTTAAAC TGGATCCCAA AATGACTCAC AAGAAGTTGA AGATTTCCAA TGATGGATTG 
CAGATGGAGA AGGAAGAAAG CTCTCTGAAG AAGAGCCATA CTCCAGAGAG ATTTAGTGGC 
ACTGGGTGCT ATGGGGCAGC AGGAAATATA TTCATTGACA GCGGTTGCCA CTACTGGGAG 
GTGGTCATGG GGTCCTCCAC ATGGTATGCA GTTGGCGTTG CCTATAAATC AGCTCCCAAG 
AATGAATGGA TTGGCAAGAA CGCCTCCTCG TGGGTCTTCT CGCGATGCAA TAGTAACTTC 
GTAGTGAGAC ATAACAACAA GGAAATGCTG GTGGACGTGC CTCCACAGTT GAAGCGCCTG 
GGTGTCCTTC TGGATTATGA CAACAACATG CTGTCTTTCT ATGACCCAGC TAACTCTCTC 
CATCTTCATA CTTTTGATGT GACCTTCATT CTTCCCGTTT GTCCAACATT CACAATTTGG 
AACAAATCCC TAATGATCCT GTCTGGCTTG CCTGCCCCAG ATTTTATTGA TTACCCTGAG 
CGGCAGGAAT GCAACTGCAG GCCTCAAGAA TCCCCTTATG TTTCTGGGAT GAAAGCTTGC 
CATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MID2 ( XM_010799702.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010798004.1 Bos taurus midline 2 (MID2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa56147
Clone ID Related Accession (Same CDS sequence)	XM_010821742.2 , XM_010799702.2
Accession Version	XM_010799702.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1386bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	probable E3 ubiquitin-protein ligase MID2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014647087.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010799702.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACCTCA CCAACCTGGT TAAGCGCAAC AGTGAACTAG AAAATCAAAT GGCCAAGCTA 
ATACAGATCT GCCAGCAGGT TGAGGTGAAT ACCGCTATGC ATGAGGCAAA ACTTATGGAA 
GAATGTGACG AATTGGTAGA GATCATCCAG CAGAGGAAGC AAATGATTGC TGTCAAAATC 
AAAGAGACAA AGGTTATGAA ACTGAGAAAA TTGGCACAGC AGGTTGCTAA TTGCCGCCAG 
TGTCTTGAAC GGTCAACAGT CCTCATCAAC CAAGCTGAGC ATATCCTGAA AGAAAATGAC 
CAGGCACGGT TTCTCCAGTC TGCAAAAAAT ATCGCTGAGA GGGTCGCTAT GGCAACTGCA 
TCTTCTCAAG TTCTGATTCC AGACATCAAT TTTAATGATG CCTTTGAAAA CTTTGCTTTA 
GATTTTTCCA GAGAAAAGAA ACTGCTGGAG GGGCTAGATT ATTTAACAGC CCCCAACCCA 
CCATCTATCC GAGAGGAACT CTGTACTGCC TCCCATGACA CCATTACGGT CCACTGGATC 
TCGGATGATG AGTTCAGCAT CAGCTCCTAT GAGCTTCAGT ACACCATATT CACTGGCCAG 
GCTAACTTCA TCAGCCTGTA TAATTCTGTG GATAGCTGGA TGATCGTGCC CAACATCAAA 
CAGAACCATT ACACAGTCCA CGGACTGCAG AGTGGAACAC GCTACATCTT CATTGTTAAA 
GCCATAAACC AAGCAGGCAG CCGGAACAGT GAACCTACCC GACTCAAAAC AAACAGCCAA 
CCCTTTAAAC TGGATCCCAA AATGACTCAC AAGAAGTTGA AGATTTCCAA TGATGGATTG 
CAGATGGAGA AGGAAGAAAG CTCTCTGAAG AAGAGCCATA CTCCAGAGAG ATTTAGTGGC 
ACTGGGTGCT ATGGGGCAGC AGGAAATATA TTCATTGACA GCGGTTGCCA CTACTGGGAG 
GTGGTCATGG GGTCCTCCAC ATGGTATGCA GTTGGCGTTG CCTATAAATC AGCTCCCAAG 
AATGAATGGA TTGGCAAGAA CGCCTCCTCG TGGGTCTTCT CGCGATGCAA TAGTAACTTC 
GTAGTGAGAC ATAACAACAA GGAAATGCTG GTGGACGTGC CTCCACAGTT GAAGCGCCTG 
GGTGTCCTTC TGGATTATGA CAACAACATG CTGTCTTTCT ATGACCCAGC TAACTCTCTC 
CATCTTCATA CTTTTGATGT GACCTTCATT CTTCCCGTTT GTCCAACATT CACAATTTGG 
AACAAATCCC TAATGATCCT GTCTGGCTTG CCTGCCCCAG ATTTTATTGA TTACCCTGAG 
CGGCAGGAAT GCAACTGCAG GCCTCAAGAA TCCCCTTATG TTTCTGGGAT GAAAGCTTGC 
CATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010798004.1
CDS	2055..3440
Translation	MNLTNLVKRNSELENQMAKLIQICQQVEVNTAMHEAKLMEECDELVEIIQQRKQMIAVKIKETKVMKLRKLAQQVANCRQCLERSTVLINQAEHILKENDQARFLQSAKNIAERVAMATASSQVLIPDINFNDAFENFALDFSREKKLLEGLDYLTAPNPPSIREELCTASHDTITVHWISDDEFSISSYELQYTIFTGQANFISLYNSVDSWMIVPNIKQNHYTVHGLQSGTRYIFIVKAINQAGSRNSEPTRLKTNSQPFKLDPKMTHKKLKISNDGLQMEKEESSLKKSHTPERFSGTGCYGAAGNIFIDSGCHYWEVVMGSSTWYAVGVAYKSAPKNEWIGKNASSWVFSRCNSNFVVRHNNKEMLVDVPPQLKRLGVLLDYDNNMLSFYDPANSLHLHTFDVTFILPVCPTFTIWNKSLMILSGLPAPDFIDYPERQECNCRPQESPYVSGMKACH

Target ORF information:

RefSeq Version	XM_010799702.2
Organism	Bos taurus(cattle)
Definition	Bos taurus midline 2 (MID2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010799702.2

ORF Insert Sequence:

ATGAACCTCA CCAACCTGGT TAAGCGCAAC AGTGAACTAG AAAATCAAAT GGCCAAGCTA 
ATACAGATCT GCCAGCAGGT TGAGGTGAAT ACCGCTATGC ATGAGGCAAA ACTTATGGAA 
GAATGTGACG AATTGGTAGA GATCATCCAG CAGAGGAAGC AAATGATTGC TGTCAAAATC 
AAAGAGACAA AGGTTATGAA ACTGAGAAAA TTGGCACAGC AGGTTGCTAA TTGCCGCCAG 
TGTCTTGAAC GGTCAACAGT CCTCATCAAC CAAGCTGAGC ATATCCTGAA AGAAAATGAC 
CAGGCACGGT TTCTCCAGTC TGCAAAAAAT ATCGCTGAGA GGGTCGCTAT GGCAACTGCA 
TCTTCTCAAG TTCTGATTCC AGACATCAAT TTTAATGATG CCTTTGAAAA CTTTGCTTTA 
GATTTTTCCA GAGAAAAGAA ACTGCTGGAG GGGCTAGATT ATTTAACAGC CCCCAACCCA 
CCATCTATCC GAGAGGAACT CTGTACTGCC TCCCATGACA CCATTACGGT CCACTGGATC 
TCGGATGATG AGTTCAGCAT CAGCTCCTAT GAGCTTCAGT ACACCATATT CACTGGCCAG 
GCTAACTTCA TCAGCCTGTA TAATTCTGTG GATAGCTGGA TGATCGTGCC CAACATCAAA 
CAGAACCATT ACACAGTCCA CGGACTGCAG AGTGGAACAC GCTACATCTT CATTGTTAAA 
GCCATAAACC AAGCAGGCAG CCGGAACAGT GAACCTACCC GACTCAAAAC AAACAGCCAA 
CCCTTTAAAC TGGATCCCAA AATGACTCAC AAGAAGTTGA AGATTTCCAA TGATGGATTG 
CAGATGGAGA AGGAAGAAAG CTCTCTGAAG AAGAGCCATA CTCCAGAGAG ATTTAGTGGC 
ACTGGGTGCT ATGGGGCAGC AGGAAATATA TTCATTGACA GCGGTTGCCA CTACTGGGAG 
GTGGTCATGG GGTCCTCCAC ATGGTATGCA GTTGGCGTTG CCTATAAATC AGCTCCCAAG 
AATGAATGGA TTGGCAAGAA CGCCTCCTCG TGGGTCTTCT CGCGATGCAA TAGTAACTTC 
GTAGTGAGAC ATAACAACAA GGAAATGCTG GTGGACGTGC CTCCACAGTT GAAGCGCCTG 
GGTGTCCTTC TGGATTATGA CAACAACATG CTGTCTTTCT ATGACCCAGC TAACTCTCTC 
CATCTTCATA CTTTTGATGT GACCTTCATT CTTCCCGTTT GTCCAACATT CACAATTTGG 
AACAAATCCC TAATGATCCT GTCTGGCTTG CCTGCCCCAG ATTTTATTGA TTACCCTGAG 
CGGCAGGAAT GCAACTGCAG GCCTCAAGAA TCCCCTTATG TTTCTGGGAT GAAAGCTTGC 
CATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MID2 ( XM_015470356.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015325842.1 Bos taurus midline 2 (MID2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa89317
Clone ID Related Accession (Same CDS sequence)	XM_015470356.1 , XM_015461593.1
Accession Version	XM_015470356.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1476bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	probable E3 ubiquitin-protein ligase MID2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014647087.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACCTCA CCAACCTGGT TAAGCGCAAC AGTGAACTAG AAAATCAAAT GGCCAAGCTA 
ATACAGATCT GCCAGCAGGT TGAGGTGAAT ACCGCTATGC ATGAGGCAAA ACTTATGGAA 
GAATGTGACG AATTGGTAGA GATCATCCAG CAGAGGAAGC AAATGATTGC TGTCAAAATC 
AAAGAGACAA AGGTTATGAA ACTGAGAAAA TTGGCACAGC AGGTTGCTAA TTGCCGCCAG 
TGTCTTGAAC GGTCAACAGT CCTCATCAAC CAAGCTGAGC ATATCCTGAA AGAAAATGAC 
CAGGCACGGT TTCTCCAGTC TGCAAAAAAT ATCGCTGAGA GGGTCGCTAT GGCAACTGCA 
TCTTCTCAAG TTCTGATTCC AGACATCAAT TTTAATGATG CCTTTGAAAA CTTTGCTTTA 
GATTTTTCCA GAGAAAAGAA ACTGCTGGAG GGGCTAGATT ATTTAACAGC CCCCAACCCA 
CCATCTATCC GAGAGGAACT CTGTACTGCC TCCCATGACA CCATTACGGT CCACTGGATC 
TCGGATGATG AGTTCAGCAT CAGCTCCTAT GAGCTTCAGT ACACCATATT CACTGGCCAG 
GCTAACTTCA TCAGTAAGTC ATGGTGTAGT TGGGGCCTGT GGCCAGAGAT AAGGAAATGT 
AAGGAAGCAG TAAGCTGCTC AAGATTGGCC GGGGCGCCAC GAGGCCTGTA TAATTCTGTG 
GATAGCTGGA TGATCGTGCC CAACATCAAA CAGAACCATT ACACAGTCCA CGGACTGCAG 
AGTGGAACAC GCTACATCTT CATTGTTAAA GCCATAAACC AAGCAGGCAG CCGGAACAGT 
GAACCTACCC GACTCAAAAC AAACAGCCAA CCCTTTAAAC TGGATCCCAA AATGACTCAC 
AAGAAGTTGA AGATTTCCAA TGATGGATTG CAGATGGAGA AGGAAGAAAG CTCTCTGAAG 
AAGAGCCATA CTCCAGAGAG ATTTAGTGGC ACTGGGTGCT ATGGGGCAGC AGGAAATATA 
TTCATTGACA GCGGTTGCCA CTACTGGGAG GTGGTCATGG GGTCCTCCAC ATGGTATGCA 
GTTGGCGTTG CCTATAAATC AGCTCCCAAG AATGAATGGA TTGGCAAGAA CGCCTCCTCG 
TGGGTCTTCT CGCGATGCAA TAGTAACTTC GTAGTGAGAC ATAACAACAA GGAAATGCTG 
GTGGACGTGC CTCCACAGTT GAAGCGCCTG GGTGTCCTTC TGGATTATGA CAACAACATG 
CTGTCTTTCT ATGACCCAGC TAACTCTCTC CATCTTCATA CTTTTGATGT GACCTTCATT 
CTTCCCGTTT GTCCAACATT CACAATTTGG AACAAATCCC TAATGATCCT GTCTGGCTTG 
CCTGCCCCAG ATTTTATTGA TTACCCTGAG CGGCAGGAAT GCAACTGCAG GCCTCAAGAA 
TCCCCTTATG TTTCTGGGAT GAAAGCTTGC CATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015325842.1
CDS	2058..3533
Translation	MNLTNLVKRNSELENQMAKLIQICQQVEVNTAMHEAKLMEECDELVEIIQQRKQMIAVKIKETKVMKLRKLAQQVANCRQCLERSTVLINQAEHILKENDQARFLQSAKNIAERVAMATASSQVLIPDINFNDAFENFALDFSREKKLLEGLDYLTAPNPPSIREELCTASHDTITVHWISDDEFSISSYELQYTIFTGQANFISKSWCSWGLWPEIRKCKEAVSCSRLAGAPRGLYNSVDSWMIVPNIKQNHYTVHGLQSGTRYIFIVKAINQAGSRNSEPTRLKTNSQPFKLDPKMTHKKLKISNDGLQMEKEESSLKKSHTPERFSGTGCYGAAGNIFIDSGCHYWEVVMGSSTWYAVGVAYKSAPKNEWIGKNASSWVFSRCNSNFVVRHNNKEMLVDVPPQLKRLGVLLDYDNNMLSFYDPANSLHLHTFDVTFILPVCPTFTIWNKSLMILSGLPAPDFIDYPERQECNCRPQESPYVSGMKACH

Target ORF information:

RefSeq Version	XM_015470356.1
Organism	Bos taurus(cattle)
Definition	Bos taurus midline 2 (MID2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015470356.1

ORF Insert Sequence:

ATGAACCTCA CCAACCTGGT TAAGCGCAAC AGTGAACTAG AAAATCAAAT GGCCAAGCTA 
ATACAGATCT GCCAGCAGGT TGAGGTGAAT ACCGCTATGC ATGAGGCAAA ACTTATGGAA 
GAATGTGACG AATTGGTAGA GATCATCCAG CAGAGGAAGC AAATGATTGC TGTCAAAATC 
AAAGAGACAA AGGTTATGAA ACTGAGAAAA TTGGCACAGC AGGTTGCTAA TTGCCGCCAG 
TGTCTTGAAC GGTCAACAGT CCTCATCAAC CAAGCTGAGC ATATCCTGAA AGAAAATGAC 
CAGGCACGGT TTCTCCAGTC TGCAAAAAAT ATCGCTGAGA GGGTCGCTAT GGCAACTGCA 
TCTTCTCAAG TTCTGATTCC AGACATCAAT TTTAATGATG CCTTTGAAAA CTTTGCTTTA 
GATTTTTCCA GAGAAAAGAA ACTGCTGGAG GGGCTAGATT ATTTAACAGC CCCCAACCCA 
CCATCTATCC GAGAGGAACT CTGTACTGCC TCCCATGACA CCATTACGGT CCACTGGATC 
TCGGATGATG AGTTCAGCAT CAGCTCCTAT GAGCTTCAGT ACACCATATT CACTGGCCAG 
GCTAACTTCA TCAGTAAGTC ATGGTGTAGT TGGGGCCTGT GGCCAGAGAT AAGGAAATGT 
AAGGAAGCAG TAAGCTGCTC AAGATTGGCC GGGGCGCCAC GAGGCCTGTA TAATTCTGTG 
GATAGCTGGA TGATCGTGCC CAACATCAAA CAGAACCATT ACACAGTCCA CGGACTGCAG 
AGTGGAACAC GCTACATCTT CATTGTTAAA GCCATAAACC AAGCAGGCAG CCGGAACAGT 
GAACCTACCC GACTCAAAAC AAACAGCCAA CCCTTTAAAC TGGATCCCAA AATGACTCAC 
AAGAAGTTGA AGATTTCCAA TGATGGATTG CAGATGGAGA AGGAAGAAAG CTCTCTGAAG 
AAGAGCCATA CTCCAGAGAG ATTTAGTGGC ACTGGGTGCT ATGGGGCAGC AGGAAATATA 
TTCATTGACA GCGGTTGCCA CTACTGGGAG GTGGTCATGG GGTCCTCCAC ATGGTATGCA 
GTTGGCGTTG CCTATAAATC AGCTCCCAAG AATGAATGGA TTGGCAAGAA CGCCTCCTCG 
TGGGTCTTCT CGCGATGCAA TAGTAACTTC GTAGTGAGAC ATAACAACAA GGAAATGCTG 
GTGGACGTGC CTCCACAGTT GAAGCGCCTG GGTGTCCTTC TGGATTATGA CAACAACATG 
CTGTCTTTCT ATGACCCAGC TAACTCTCTC CATCTTCATA CTTTTGATGT GACCTTCATT 
CTTCCCGTTT GTCCAACATT CACAATTTGG AACAAATCCC TAATGATCCT GTCTGGCTTG 
CCTGCCCCAG ATTTTATTGA TTACCCTGAG CGGCAGGAAT GCAACTGCAG GCCTCAAGAA 
TCCCCTTATG TTTCTGGGAT GAAAGCTTGC CATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MID2 ( XM_015461593.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015317079.1 Bos taurus midline 2 (MID2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa89317
Clone ID Related Accession (Same CDS sequence)	XM_015470356.1 , XM_015461593.1
Accession Version	XM_015461593.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1476bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	probable E3 ubiquitin-protein ligase MID2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104715.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACCTCA CCAACCTGGT TAAGCGCAAC AGTGAACTAG AAAATCAAAT GGCCAAGCTA 
ATACAGATCT GCCAGCAGGT TGAGGTGAAT ACCGCTATGC ATGAGGCAAA ACTTATGGAA 
GAATGTGACG AATTGGTAGA GATCATCCAG CAGAGGAAGC AAATGATTGC TGTCAAAATC 
AAAGAGACAA AGGTTATGAA ACTGAGAAAA TTGGCACAGC AGGTTGCTAA TTGCCGCCAG 
TGTCTTGAAC GGTCAACAGT CCTCATCAAC CAAGCTGAGC ATATCCTGAA AGAAAATGAC 
CAGGCACGGT TTCTCCAGTC TGCAAAAAAT ATCGCTGAGA GGGTCGCTAT GGCAACTGCA 
TCTTCTCAAG TTCTGATTCC AGACATCAAT TTTAATGATG CCTTTGAAAA CTTTGCTTTA 
GATTTTTCCA GAGAAAAGAA ACTGCTGGAG GGGCTAGATT ATTTAACAGC CCCCAACCCA 
CCATCTATCC GAGAGGAACT CTGTACTGCC TCCCATGACA CCATTACGGT CCACTGGATC 
TCGGATGATG AGTTCAGCAT CAGCTCCTAT GAGCTTCAGT ACACCATATT CACTGGCCAG 
GCTAACTTCA TCAGTAAGTC ATGGTGTAGT TGGGGCCTGT GGCCAGAGAT AAGGAAATGT 
AAGGAAGCAG TAAGCTGCTC AAGATTGGCC GGGGCGCCAC GAGGCCTGTA TAATTCTGTG 
GATAGCTGGA TGATCGTGCC CAACATCAAA CAGAACCATT ACACAGTCCA CGGACTGCAG 
AGTGGAACAC GCTACATCTT CATTGTTAAA GCCATAAACC AAGCAGGCAG CCGGAACAGT 
GAACCTACCC GACTCAAAAC AAACAGCCAA CCCTTTAAAC TGGATCCCAA AATGACTCAC 
AAGAAGTTGA AGATTTCCAA TGATGGATTG CAGATGGAGA AGGAAGAAAG CTCTCTGAAG 
AAGAGCCATA CTCCAGAGAG ATTTAGTGGC ACTGGGTGCT ATGGGGCAGC AGGAAATATA 
TTCATTGACA GCGGTTGCCA CTACTGGGAG GTGGTCATGG GGTCCTCCAC ATGGTATGCA 
GTTGGCGTTG CCTATAAATC AGCTCCCAAG AATGAATGGA TTGGCAAGAA CGCCTCCTCG 
TGGGTCTTCT CGCGATGCAA TAGTAACTTC GTAGTGAGAC ATAACAACAA GGAAATGCTG 
GTGGACGTGC CTCCACAGTT GAAGCGCCTG GGTGTCCTTC TGGATTATGA CAACAACATG 
CTGTCTTTCT ATGACCCAGC TAACTCTCTC CATCTTCATA CTTTTGATGT GACCTTCATT 
CTTCCCGTTT GTCCAACATT CACAATTTGG AACAAATCCC TAATGATCCT GTCTGGCTTG 
CCTGCCCCAG ATTTTATTGA TTACCCTGAG CGGCAGGAAT GCAACTGCAG GCCTCAAGAA 
TCCCCTTATG TTTCTGGGAT GAAAGCTTGC CATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015317079.1
CDS	2058..3533
Translation	MNLTNLVKRNSELENQMAKLIQICQQVEVNTAMHEAKLMEECDELVEIIQQRKQMIAVKIKETKVMKLRKLAQQVANCRQCLERSTVLINQAEHILKENDQARFLQSAKNIAERVAMATASSQVLIPDINFNDAFENFALDFSREKKLLEGLDYLTAPNPPSIREELCTASHDTITVHWISDDEFSISSYELQYTIFTGQANFISKSWCSWGLWPEIRKCKEAVSCSRLAGAPRGLYNSVDSWMIVPNIKQNHYTVHGLQSGTRYIFIVKAINQAGSRNSEPTRLKTNSQPFKLDPKMTHKKLKISNDGLQMEKEESSLKKSHTPERFSGTGCYGAAGNIFIDSGCHYWEVVMGSSTWYAVGVAYKSAPKNEWIGKNASSWVFSRCNSNFVVRHNNKEMLVDVPPQLKRLGVLLDYDNNMLSFYDPANSLHLHTFDVTFILPVCPTFTIWNKSLMILSGLPAPDFIDYPERQECNCRPQESPYVSGMKACH

Target ORF information:

RefSeq Version	XM_015461593.1
Organism	Bos taurus(cattle)
Definition	Bos taurus midline 2 (MID2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015461593.1

ORF Insert Sequence:

ATGAACCTCA CCAACCTGGT TAAGCGCAAC AGTGAACTAG AAAATCAAAT GGCCAAGCTA 
ATACAGATCT GCCAGCAGGT TGAGGTGAAT ACCGCTATGC ATGAGGCAAA ACTTATGGAA 
GAATGTGACG AATTGGTAGA GATCATCCAG CAGAGGAAGC AAATGATTGC TGTCAAAATC 
AAAGAGACAA AGGTTATGAA ACTGAGAAAA TTGGCACAGC AGGTTGCTAA TTGCCGCCAG 
TGTCTTGAAC GGTCAACAGT CCTCATCAAC CAAGCTGAGC ATATCCTGAA AGAAAATGAC 
CAGGCACGGT TTCTCCAGTC TGCAAAAAAT ATCGCTGAGA GGGTCGCTAT GGCAACTGCA 
TCTTCTCAAG TTCTGATTCC AGACATCAAT TTTAATGATG CCTTTGAAAA CTTTGCTTTA 
GATTTTTCCA GAGAAAAGAA ACTGCTGGAG GGGCTAGATT ATTTAACAGC CCCCAACCCA 
CCATCTATCC GAGAGGAACT CTGTACTGCC TCCCATGACA CCATTACGGT CCACTGGATC 
TCGGATGATG AGTTCAGCAT CAGCTCCTAT GAGCTTCAGT ACACCATATT CACTGGCCAG 
GCTAACTTCA TCAGTAAGTC ATGGTGTAGT TGGGGCCTGT GGCCAGAGAT AAGGAAATGT 
AAGGAAGCAG TAAGCTGCTC AAGATTGGCC GGGGCGCCAC GAGGCCTGTA TAATTCTGTG 
GATAGCTGGA TGATCGTGCC CAACATCAAA CAGAACCATT ACACAGTCCA CGGACTGCAG 
AGTGGAACAC GCTACATCTT CATTGTTAAA GCCATAAACC AAGCAGGCAG CCGGAACAGT 
GAACCTACCC GACTCAAAAC AAACAGCCAA CCCTTTAAAC TGGATCCCAA AATGACTCAC 
AAGAAGTTGA AGATTTCCAA TGATGGATTG CAGATGGAGA AGGAAGAAAG CTCTCTGAAG 
AAGAGCCATA CTCCAGAGAG ATTTAGTGGC ACTGGGTGCT ATGGGGCAGC AGGAAATATA 
TTCATTGACA GCGGTTGCCA CTACTGGGAG GTGGTCATGG GGTCCTCCAC ATGGTATGCA 
GTTGGCGTTG CCTATAAATC AGCTCCCAAG AATGAATGGA TTGGCAAGAA CGCCTCCTCG 
TGGGTCTTCT CGCGATGCAA TAGTAACTTC GTAGTGAGAC ATAACAACAA GGAAATGCTG 
GTGGACGTGC CTCCACAGTT GAAGCGCCTG GGTGTCCTTC TGGATTATGA CAACAACATG 
CTGTCTTTCT ATGACCCAGC TAACTCTCTC CATCTTCATA CTTTTGATGT GACCTTCATT 
CTTCCCGTTT GTCCAACATT CACAATTTGG AACAAATCCC TAATGATCCT GTCTGGCTTG 
CCTGCCCCAG ATTTTATTGA TTACCCTGAG CGGCAGGAAT GCAACTGCAG GCCTCAAGAA 
TCCCCTTATG TTTCTGGGAT GAAAGCTTGC CATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MID2 ( XM_024988373.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024844141.1 Bos taurus midline 2 (MID2), transcript variant X1, mRNA.

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CloneID	OBa238550
Clone ID Related Accession (Same CDS sequence)	XM_024988373.1
Accession Version	XM_024988373.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2325bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	probable E3 ubiquitin-protein ligase MID2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCCTGG AGTCTCCGTC TGGGTGGTTC CTTATTTTTG GCCTAGCTGT GAAACCAGCT 
CAGTGGGGGA GAGGGAAAAC TTATAGGTCC CCCGGACAGA CTGCGATGGA ACCCGGGAAA 
GGTGAAAGCC CAGCCTCTGC GGTTCTTAAT GCCTCAGCAG GATTATTTTC ACTAAAGATG 
GAAACACTGG AGTCTGAATT GACCTGTCCA ATCTGCCTAG AGTTGTTTGA AGACCCCCTT 
CTGCTCCCTT GTGCTCACAG CCTCTGCTTC AGCTGTGCCC ATCGCATCTT GGTCTCGAGC 
TGCAGCTCCG GTGAATCCAT TGAACCCATT ACTGCTTTCC AGTGTCCTAC GTGCAGGTAT 
GTTATCTCAC TGAACCACCG GGGCCTGGAT GGCCTCAAGA GAAATGTGAC TCTGCAGAAC 
ATTATTGACC GCTTCCAGAA GGCTTCCGTC AGTGGACCCA ACTCCCCAAG TGAGAGCCGC 
CGAGAGAGGA CTTACAGGCC CAGCAGCGCC ATGTCTAGTG AGCGAATTGC GTGCCAATTC 
TGTGAGCAGG ACCCGCCAAG GGATGCAGTG AAAACGTGCA TCACTTGTGA GGTCTCTTAC 
TGTGACCGTT GCCTGCGGGC TACACACCCC AACAAGAAAC CTTTCACCAG CCACCGCCTG 
GTGGAACCAG TGCCGGACAC GCATCTTCGA GGGATCACCT GCCTGGACCA TGAAAATGAG 
AAAGTGAATA TGTACTGTGT TTCTGATGAC CAATTGATCT GTGCCTTATG CAAACTGGTG 
GGGCGTCACC GAGACCATCA GGTCGCATCC CTGAATGATC GGTTTGAGAA GCTCAAGCAA 
ACTCTGGAGA TGAACCTCAC CAACCTGGTT AAGCGCAACA GTGAACTAGA AAATCAAATG 
GCCAAGCTAA TACAGATCTG CCAGCAGGTT GAGGTGAATA CCGCTATGCA TGAGGCAAAA 
CTTATGGAAG AATGTGACGA ATTGGTAGAG ATCATCCAGC AGAGGAAGCA AATGATTGCT 
GTCAAAATCA AAGAGACAAA GGTTATGAAA CTGAGAAAAT TGGCACAGCA GGTTGCTAAT 
TGCCGCCAGT GTCTTGAACG GTCAACAGTC CTCATCAACC AAGCTGAGCA TATCCTGAAA 
GAAAATGACC AGGCACGGTT TCTCCAGTCT GCAAAAAATA TCGCTGAGAG GGTCGCTATG 
GCAACTGCAT CTTCTCAAGT TCTGATTCCA GACATCAATT TTAATGATGC CTTTGAAAAC 
TTTGCTTTAG ATTTTTCCAG AGAAAAGAAA CTGCTGGAGG GGCTAGATTA TTTAACAGCC 
CCCAACCCAC CATCTATCCG AGAGGAACTC TGTACTGCCT CCCATGACAC CATTACGGTC 
CACTGGATCT CGGATGATGA GTTCAGCATC AGCTCCTATG AGCTTCAGTA CACCATATTC 
ACTGGCCAGG CTAACTTCAT CAGTAAGTCA TGGTGTAGTT GGGGCCTGTG GCCAGAGATA 
AGGAAATGTA AGGAAGCAGT AAGCTGCTCA AGATTGGCCG GGGCGCCACG AGGCCTGTAT 
AATTCTGTGG ATAGCTGGAT GATCGTGCCC AACATCAAAC AGAACCATTA CACAGTCCAC 
GGACTGCAGA GTGGAACACG CTACATCTTC ATTGTTAAAG CCATAAACCA AGCAGGCAGC 
CGGAACAGTG AACCTACCCG ACTCAAAACA AACAGCCAAC CCTTTAAACT GGATCCCAAA 
ATGACTCACA AGAAGTTGAA GATTTCCAAT GATGGATTGC AGATGGAGAA GGAAGAAAGC 
TCTCTGAAGA AGAGCCATAC TCCAGAGAGA TTTAGTGGCA CTGGGTGCTA TGGGGCAGCA 
GGAAATATAT TCATTGACAG CGGTTGCCAC TACTGGGAGG TGGTCATGGG GTCCTCCACA 
TGGTATGCAG TTGGCGTTGC CTATAAATCA GCTCCCAAGA ATGAATGGAT TGGCAAGAAC 
GCCTCCTCGT GGGTCTTCTC GCGATGCAAT AGTAACTTCG TAGTGAGACA TAACAACAAG 
GAAATGCTGG TGGACGTGCC TCCACAGTTG AAGCGCCTGG GTGTCCTTCT GGATTATGAC 
AACAACATGC TGTCTTTCTA TGACCCAGCT AACTCTCTCC ATCTTCATAC TTTTGATGTG 
ACCTTCATTC TTCCCGTTTG TCCAACATTC ACAATTTGGA ACAAATCCCT AATGATCCTG 
TCTGGCTTGC CTGCCCCAGA TTTTATTGAT TACCCTGAGC GGCAGGAATG CAACTGCAGG 
CCTCAAGAAT CCCCTTATGT TTCTGGGATG AAAGCTTGCC ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024844141.1
CDS	108..2432
Translation	MILESPSGWFLIFGLAVKPAQWGRGKTYRSPGQTAMEPGKGESPASAVLNASAGLFSLKMETLESELTCPICLELFEDPLLLPCAHSLCFSCAHRILVSSCSSGESIEPITAFQCPTCRYVISLNHRGLDGLKRNVTLQNIIDRFQKASVSGPNSPSESRRERTYRPSSAMSSERIACQFCEQDPPRDAVKTCITCEVSYCDRCLRATHPNKKPFTSHRLVEPVPDTHLRGITCLDHENEKVNMYCVSDDQLICALCKLVGRHRDHQVASLNDRFEKLKQTLEMNLTNLVKRNSELENQMAKLIQICQQVEVNTAMHEAKLMEECDELVEIIQQRKQMIAVKIKETKVMKLRKLAQQVANCRQCLERSTVLINQAEHILKENDQARFLQSAKNIAERVAMATASSQVLIPDINFNDAFENFALDFSREKKLLEGLDYLTAPNPPSIREELCTASHDTITVHWISDDEFSISSYELQYTIFTGQANFISKSWCSWGLWPEIRKCKEAVSCSRLAGAPRGLYNSVDSWMIVPNIKQNHYTVHGLQSGTRYIFIVKAINQAGSRNSEPTRLKTNSQPFKLDPKMTHKKLKISNDGLQMEKEESSLKKSHTPERFSGTGCYGAAGNIFIDSGCHYWEVVMGSSTWYAVGVAYKSAPKNEWIGKNASSWVFSRCNSNFVVRHNNKEMLVDVPPQLKRLGVLLDYDNNMLSFYDPANSLHLHTFDVTFILPVCPTFTIWNKSLMILSGLPAPDFIDYPERQECNCRPQESPYVSGMKACH

Target ORF information:

RefSeq Version	XM_024988373.1
Organism	Bos taurus(cattle)
Definition	Bos taurus midline 2 (MID2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024988373.1

ORF Insert Sequence:

ATGATCCTGG AGTCTCCGTC TGGGTGGTTC CTTATTTTTG GCCTAGCTGT GAAACCAGCT 
CAGTGGGGGA GAGGGAAAAC TTATAGGTCC CCCGGACAGA CTGCGATGGA ACCCGGGAAA 
GGTGAAAGCC CAGCCTCTGC GGTTCTTAAT GCCTCAGCAG GATTATTTTC ACTAAAGATG 
GAAACACTGG AGTCTGAATT GACCTGTCCA ATCTGCCTAG AGTTGTTTGA AGACCCCCTT 
CTGCTCCCTT GTGCTCACAG CCTCTGCTTC AGCTGTGCCC ATCGCATCTT GGTCTCGAGC 
TGCAGCTCCG GTGAATCCAT TGAACCCATT ACTGCTTTCC AGTGTCCTAC GTGCAGGTAT 
GTTATCTCAC TGAACCACCG GGGCCTGGAT GGCCTCAAGA GAAATGTGAC TCTGCAGAAC 
ATTATTGACC GCTTCCAGAA GGCTTCCGTC AGTGGACCCA ACTCCCCAAG TGAGAGCCGC 
CGAGAGAGGA CTTACAGGCC CAGCAGCGCC ATGTCTAGTG AGCGAATTGC GTGCCAATTC 
TGTGAGCAGG ACCCGCCAAG GGATGCAGTG AAAACGTGCA TCACTTGTGA GGTCTCTTAC 
TGTGACCGTT GCCTGCGGGC TACACACCCC AACAAGAAAC CTTTCACCAG CCACCGCCTG 
GTGGAACCAG TGCCGGACAC GCATCTTCGA GGGATCACCT GCCTGGACCA TGAAAATGAG 
AAAGTGAATA TGTACTGTGT TTCTGATGAC CAATTGATCT GTGCCTTATG CAAACTGGTG 
GGGCGTCACC GAGACCATCA GGTCGCATCC CTGAATGATC GGTTTGAGAA GCTCAAGCAA 
ACTCTGGAGA TGAACCTCAC CAACCTGGTT AAGCGCAACA GTGAACTAGA AAATCAAATG 
GCCAAGCTAA TACAGATCTG CCAGCAGGTT GAGGTGAATA CCGCTATGCA TGAGGCAAAA 
CTTATGGAAG AATGTGACGA ATTGGTAGAG ATCATCCAGC AGAGGAAGCA AATGATTGCT 
GTCAAAATCA AAGAGACAAA GGTTATGAAA CTGAGAAAAT TGGCACAGCA GGTTGCTAAT 
TGCCGCCAGT GTCTTGAACG GTCAACAGTC CTCATCAACC AAGCTGAGCA TATCCTGAAA 
GAAAATGACC AGGCACGGTT TCTCCAGTCT GCAAAAAATA TCGCTGAGAG GGTCGCTATG 
GCAACTGCAT CTTCTCAAGT TCTGATTCCA GACATCAATT TTAATGATGC CTTTGAAAAC 
TTTGCTTTAG ATTTTTCCAG AGAAAAGAAA CTGCTGGAGG GGCTAGATTA TTTAACAGCC 
CCCAACCCAC CATCTATCCG AGAGGAACTC TGTACTGCCT CCCATGACAC CATTACGGTC 
CACTGGATCT CGGATGATGA GTTCAGCATC AGCTCCTATG AGCTTCAGTA CACCATATTC 
ACTGGCCAGG CTAACTTCAT CAGTAAGTCA TGGTGTAGTT GGGGCCTGTG GCCAGAGATA 
AGGAAATGTA AGGAAGCAGT AAGCTGCTCA AGATTGGCCG GGGCGCCACG AGGCCTGTAT 
AATTCTGTGG ATAGCTGGAT GATCGTGCCC AACATCAAAC AGAACCATTA CACAGTCCAC 
GGACTGCAGA GTGGAACACG CTACATCTTC ATTGTTAAAG CCATAAACCA AGCAGGCAGC 
CGGAACAGTG AACCTACCCG ACTCAAAACA AACAGCCAAC CCTTTAAACT GGATCCCAAA 
ATGACTCACA AGAAGTTGAA GATTTCCAAT GATGGATTGC AGATGGAGAA GGAAGAAAGC 
TCTCTGAAGA AGAGCCATAC TCCAGAGAGA TTTAGTGGCA CTGGGTGCTA TGGGGCAGCA 
GGAAATATAT TCATTGACAG CGGTTGCCAC TACTGGGAGG TGGTCATGGG GTCCTCCACA 
TGGTATGCAG TTGGCGTTGC CTATAAATCA GCTCCCAAGA ATGAATGGAT TGGCAAGAAC 
GCCTCCTCGT GGGTCTTCTC GCGATGCAAT AGTAACTTCG TAGTGAGACA TAACAACAAG 
GAAATGCTGG TGGACGTGCC TCCACAGTTG AAGCGCCTGG GTGTCCTTCT GGATTATGAC 
AACAACATGC TGTCTTTCTA TGACCCAGCT AACTCTCTCC ATCTTCATAC TTTTGATGTG 
ACCTTCATTC TTCCCGTTTG TCCAACATTC ACAATTTGGA ACAAATCCCT AATGATCCTG 
TCTGGCTTGC CTGCCCCAGA TTTTATTGAT TACCCTGAGC GGCAGGAATG CAACTGCAGG 
CCTCAAGAAT CCCCTTATGT TTCTGGGATG AAAGCTTGCC ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MID2 ( XM_010821742.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010820044.2 Bos taurus midline 2 (MID2), transcript variant X2, mRNA.

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CloneID	OBa238551
Clone ID Related Accession (Same CDS sequence)	XM_010821742.3
Accession Version	XM_010821742.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2235bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	probable E3 ubiquitin-protein ligase MID2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010821742.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCCTGG AGTCTCCGTC TGGGTGGTTC CTTATTTTTG GCCTAGCTGT GAAACCAGCT 
CAGTGGGGGA GAGGGAAAAC TTATAGGTCC CCCGGACAGA CTGCGATGGA ACCCGGGAAA 
GGTGAAAGCC CAGCCTCTGC GGTTCTTAAT GCCTCAGCAG GATTATTTTC ACTAAAGATG 
GAAACACTGG AGTCTGAATT GACCTGTCCA ATCTGCCTAG AGTTGTTTGA AGACCCCCTT 
CTGCTCCCTT GTGCTCACAG CCTCTGCTTC AGCTGTGCCC ATCGCATCTT GGTCTCGAGC 
TGCAGCTCCG GTGAATCCAT TGAACCCATT ACTGCTTTCC AGTGTCCTAC GTGCAGGTAT 
GTTATCTCAC TGAACCACCG GGGCCTGGAT GGCCTCAAGA GAAATGTGAC TCTGCAGAAC 
ATTATTGACC GCTTCCAGAA GGCTTCCGTC AGTGGACCCA ACTCCCCAAG TGAGAGCCGC 
CGAGAGAGGA CTTACAGGCC CAGCAGCGCC ATGTCTAGTG AGCGAATTGC GTGCCAATTC 
TGTGAGCAGG ACCCGCCAAG GGATGCAGTG AAAACGTGCA TCACTTGTGA GGTCTCTTAC 
TGTGACCGTT GCCTGCGGGC TACACACCCC AACAAGAAAC CTTTCACCAG CCACCGCCTG 
GTGGAACCAG TGCCGGACAC GCATCTTCGA GGGATCACCT GCCTGGACCA TGAAAATGAG 
AAAGTGAATA TGTACTGTGT TTCTGATGAC CAATTGATCT GTGCCTTATG CAAACTGGTG 
GGGCGTCACC GAGACCATCA GGTCGCATCC CTGAATGATC GGTTTGAGAA GCTCAAGCAA 
ACTCTGGAGA TGAACCTCAC CAACCTGGTT AAGCGCAACA GTGAACTAGA AAATCAAATG 
GCCAAGCTAA TACAGATCTG CCAGCAGGTT GAGGTGAATA CCGCTATGCA TGAGGCAAAA 
CTTATGGAAG AATGTGACGA ATTGGTAGAG ATCATCCAGC AGAGGAAGCA AATGATTGCT 
GTCAAAATCA AAGAGACAAA GGTTATGAAA CTGAGAAAAT TGGCACAGCA GGTTGCTAAT 
TGCCGCCAGT GTCTTGAACG GTCAACAGTC CTCATCAACC AAGCTGAGCA TATCCTGAAA 
GAAAATGACC AGGCACGGTT TCTCCAGTCT GCAAAAAATA TCGCTGAGAG GGTCGCTATG 
GCAACTGCAT CTTCTCAAGT TCTGATTCCA GACATCAATT TTAATGATGC CTTTGAAAAC 
TTTGCTTTAG ATTTTTCCAG AGAAAAGAAA CTGCTGGAGG GGCTAGATTA TTTAACAGCC 
CCCAACCCAC CATCTATCCG AGAGGAACTC TGTACTGCCT CCCATGACAC CATTACGGTC 
CACTGGATCT CGGATGATGA GTTCAGCATC AGCTCCTATG AGCTTCAGTA CACCATATTC 
ACTGGCCAGG CTAACTTCAT CAGCCTGTAT AATTCTGTGG ATAGCTGGAT GATCGTGCCC 
AACATCAAAC AGAACCATTA CACAGTCCAC GGACTGCAGA GTGGAACACG CTACATCTTC 
ATTGTTAAAG CCATAAACCA AGCAGGCAGC CGGAACAGTG AACCTACCCG ACTCAAAACA 
AACAGCCAAC CCTTTAAACT GGATCCCAAA ATGACTCACA AGAAGTTGAA GATTTCCAAT 
GATGGATTGC AGATGGAGAA GGAAGAAAGC TCTCTGAAGA AGAGCCATAC TCCAGAGAGA 
TTTAGTGGCA CTGGGTGCTA TGGGGCAGCA GGAAATATAT TCATTGACAG CGGTTGCCAC 
TACTGGGAGG TGGTCATGGG GTCCTCCACA TGGTATGCAG TTGGCGTTGC CTATAAATCA 
GCTCCCAAGA ATGAATGGAT TGGCAAGAAC GCCTCCTCGT GGGTCTTCTC GCGATGCAAT 
AGTAACTTCG TAGTGAGACA TAACAACAAG GAAATGCTGG TGGACGTGCC TCCACAGTTG 
AAGCGCCTGG GTGTCCTTCT GGATTATGAC AACAACATGC TGTCTTTCTA TGACCCAGCT 
AACTCTCTCC ATCTTCATAC TTTTGATGTG ACCTTCATTC TTCCCGTTTG TCCAACATTC 
ACAATTTGGA ACAAATCCCT AATGATCCTG TCTGGCTTGC CTGCCCCAGA TTTTATTGAT 
TACCCTGAGC GGCAGGAATG CAACTGCAGG CCTCAAGAAT CCCCTTATGT TTCTGGGATG 
AAAGCTTGCC ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010820044.2
CDS	100..2334
Translation	MILESPSGWFLIFGLAVKPAQWGRGKTYRSPGQTAMEPGKGESPASAVLNASAGLFSLKMETLESELTCPICLELFEDPLLLPCAHSLCFSCAHRILVSSCSSGESIEPITAFQCPTCRYVISLNHRGLDGLKRNVTLQNIIDRFQKASVSGPNSPSESRRERTYRPSSAMSSERIACQFCEQDPPRDAVKTCITCEVSYCDRCLRATHPNKKPFTSHRLVEPVPDTHLRGITCLDHENEKVNMYCVSDDQLICALCKLVGRHRDHQVASLNDRFEKLKQTLEMNLTNLVKRNSELENQMAKLIQICQQVEVNTAMHEAKLMEECDELVEIIQQRKQMIAVKIKETKVMKLRKLAQQVANCRQCLERSTVLINQAEHILKENDQARFLQSAKNIAERVAMATASSQVLIPDINFNDAFENFALDFSREKKLLEGLDYLTAPNPPSIREELCTASHDTITVHWISDDEFSISSYELQYTIFTGQANFISLYNSVDSWMIVPNIKQNHYTVHGLQSGTRYIFIVKAINQAGSRNSEPTRLKTNSQPFKLDPKMTHKKLKISNDGLQMEKEESSLKKSHTPERFSGTGCYGAAGNIFIDSGCHYWEVVMGSSTWYAVGVAYKSAPKNEWIGKNASSWVFSRCNSNFVVRHNNKEMLVDVPPQLKRLGVLLDYDNNMLSFYDPANSLHLHTFDVTFILPVCPTFTIWNKSLMILSGLPAPDFIDYPERQECNCRPQESPYVSGMKACH

Target ORF information:

RefSeq Version	XM_010821742.3
Organism	Bos taurus(cattle)
Definition	Bos taurus midline 2 (MID2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010821742.3

ORF Insert Sequence:

ATGATCCTGG AGTCTCCGTC TGGGTGGTTC CTTATTTTTG GCCTAGCTGT GAAACCAGCT 
CAGTGGGGGA GAGGGAAAAC TTATAGGTCC CCCGGACAGA CTGCGATGGA ACCCGGGAAA 
GGTGAAAGCC CAGCCTCTGC GGTTCTTAAT GCCTCAGCAG GATTATTTTC ACTAAAGATG 
GAAACACTGG AGTCTGAATT GACCTGTCCA ATCTGCCTAG AGTTGTTTGA AGACCCCCTT 
CTGCTCCCTT GTGCTCACAG CCTCTGCTTC AGCTGTGCCC ATCGCATCTT GGTCTCGAGC 
TGCAGCTCCG GTGAATCCAT TGAACCCATT ACTGCTTTCC AGTGTCCTAC GTGCAGGTAT 
GTTATCTCAC TGAACCACCG GGGCCTGGAT GGCCTCAAGA GAAATGTGAC TCTGCAGAAC 
ATTATTGACC GCTTCCAGAA GGCTTCCGTC AGTGGACCCA ACTCCCCAAG TGAGAGCCGC 
CGAGAGAGGA CTTACAGGCC CAGCAGCGCC ATGTCTAGTG AGCGAATTGC GTGCCAATTC 
TGTGAGCAGG ACCCGCCAAG GGATGCAGTG AAAACGTGCA TCACTTGTGA GGTCTCTTAC 
TGTGACCGTT GCCTGCGGGC TACACACCCC AACAAGAAAC CTTTCACCAG CCACCGCCTG 
GTGGAACCAG TGCCGGACAC GCATCTTCGA GGGATCACCT GCCTGGACCA TGAAAATGAG 
AAAGTGAATA TGTACTGTGT TTCTGATGAC CAATTGATCT GTGCCTTATG CAAACTGGTG 
GGGCGTCACC GAGACCATCA GGTCGCATCC CTGAATGATC GGTTTGAGAA GCTCAAGCAA 
ACTCTGGAGA TGAACCTCAC CAACCTGGTT AAGCGCAACA GTGAACTAGA AAATCAAATG 
GCCAAGCTAA TACAGATCTG CCAGCAGGTT GAGGTGAATA CCGCTATGCA TGAGGCAAAA 
CTTATGGAAG AATGTGACGA ATTGGTAGAG ATCATCCAGC AGAGGAAGCA AATGATTGCT 
GTCAAAATCA AAGAGACAAA GGTTATGAAA CTGAGAAAAT TGGCACAGCA GGTTGCTAAT 
TGCCGCCAGT GTCTTGAACG GTCAACAGTC CTCATCAACC AAGCTGAGCA TATCCTGAAA 
GAAAATGACC AGGCACGGTT TCTCCAGTCT GCAAAAAATA TCGCTGAGAG GGTCGCTATG 
GCAACTGCAT CTTCTCAAGT TCTGATTCCA GACATCAATT TTAATGATGC CTTTGAAAAC 
TTTGCTTTAG ATTTTTCCAG AGAAAAGAAA CTGCTGGAGG GGCTAGATTA TTTAACAGCC 
CCCAACCCAC CATCTATCCG AGAGGAACTC TGTACTGCCT CCCATGACAC CATTACGGTC 
CACTGGATCT CGGATGATGA GTTCAGCATC AGCTCCTATG AGCTTCAGTA CACCATATTC 
ACTGGCCAGG CTAACTTCAT CAGCCTGTAT AATTCTGTGG ATAGCTGGAT GATCGTGCCC 
AACATCAAAC AGAACCATTA CACAGTCCAC GGACTGCAGA GTGGAACACG CTACATCTTC 
ATTGTTAAAG CCATAAACCA AGCAGGCAGC CGGAACAGTG AACCTACCCG ACTCAAAACA 
AACAGCCAAC CCTTTAAACT GGATCCCAAA ATGACTCACA AGAAGTTGAA GATTTCCAAT 
GATGGATTGC AGATGGAGAA GGAAGAAAGC TCTCTGAAGA AGAGCCATAC TCCAGAGAGA 
TTTAGTGGCA CTGGGTGCTA TGGGGCAGCA GGAAATATAT TCATTGACAG CGGTTGCCAC 
TACTGGGAGG TGGTCATGGG GTCCTCCACA TGGTATGCAG TTGGCGTTGC CTATAAATCA 
GCTCCCAAGA ATGAATGGAT TGGCAAGAAC GCCTCCTCGT GGGTCTTCTC GCGATGCAAT 
AGTAACTTCG TAGTGAGACA TAACAACAAG GAAATGCTGG TGGACGTGCC TCCACAGTTG 
AAGCGCCTGG GTGTCCTTCT GGATTATGAC AACAACATGC TGTCTTTCTA TGACCCAGCT 
AACTCTCTCC ATCTTCATAC TTTTGATGTG ACCTTCATTC TTCCCGTTTG TCCAACATTC 
ACAATTTGGA ACAAATCCCT AATGATCCTG TCTGGCTTGC CTGCCCCAGA TTTTATTGAT 
TACCCTGAGC GGCAGGAATG CAACTGCAGG CCTCAAGAAT CCCCTTATGT TTCTGGGATG 
AAAGCTTGCC ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MID2 ( XM_024988376.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024844144.1 Bos taurus midline 2 (MID2), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OBa238552
Clone ID Related Accession (Same CDS sequence)	XM_024988376.1 , XM_024988374.1 , XM_024988375.1
Accession Version	XM_024988376.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2148bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	probable E3 ubiquitin-protein ligase MID2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAACAC TGGAGTCTGA ATTGACCTGT CCAATCTGCC TAGAGTTGTT TGAAGACCCC 
CTTCTGCTCC CTTGTGCTCA CAGCCTCTGC TTCAGCTGTG CCCATCGCAT CTTGGTCTCG 
AGCTGCAGCT CCGGTGAATC CATTGAACCC ATTACTGCTT TCCAGTGTCC TACGTGCAGG 
TATGTTATCT CACTGAACCA CCGGGGCCTG GATGGCCTCA AGAGAAATGT GACTCTGCAG 
AACATTATTG ACCGCTTCCA GAAGGCTTCC GTCAGTGGAC CCAACTCCCC AAGTGAGAGC 
CGCCGAGAGA GGACTTACAG GCCCAGCAGC GCCATGTCTA GTGAGCGAAT TGCGTGCCAA 
TTCTGTGAGC AGGACCCGCC AAGGGATGCA GTGAAAACGT GCATCACTTG TGAGGTCTCT 
TACTGTGACC GTTGCCTGCG GGCTACACAC CCCAACAAGA AACCTTTCAC CAGCCACCGC 
CTGGTGGAAC CAGTGCCGGA CACGCATCTT CGAGGGATCA CCTGCCTGGA CCATGAAAAT 
GAGAAAGTGA ATATGTACTG TGTTTCTGAT GACCAATTGA TCTGTGCCTT ATGCAAACTG 
GTGGGGCGTC ACCGAGACCA TCAGGTCGCA TCCCTGAATG ATCGGTTTGA GAAGCTCAAG 
CAAACTCTGG AGATGAACCT CACCAACCTG GTTAAGCGCA ACAGTGAACT AGAAAATCAA 
ATGGCCAAGC TAATACAGAT CTGCCAGCAG GTTGAGGTGA ATACCGCTAT GCATGAGGCA 
AAACTTATGG AAGAATGTGA CGAATTGGTA GAGATCATCC AGCAGAGGAA GCAAATGATT 
GCTGTCAAAA TCAAAGAGAC AAAGGTTATG AAACTGAGAA AATTGGCACA GCAGGTTGCT 
AATTGCCGCC AGTGTCTTGA ACGGTCAACA GTCCTCATCA ACCAAGCTGA GCATATCCTG 
AAAGAAAATG ACCAGGCACG GTTTCTCCAG TCTGCAAAAA ATATCGCTGA GAGGGTCGCT 
ATGGCAACTG CATCTTCTCA AGTTCTGATT CCAGACATCA ATTTTAATGA TGCCTTTGAA 
AACTTTGCTT TAGATTTTTC CAGAGAAAAG AAACTGCTGG AGGGGCTAGA TTATTTAACA 
GCCCCCAACC CACCATCTAT CCGAGAGGAA CTCTGTACTG CCTCCCATGA CACCATTACG 
GTCCACTGGA TCTCGGATGA TGAGTTCAGC ATCAGCTCCT ATGAGCTTCA GTACACCATA 
TTCACTGGCC AGGCTAACTT CATCAGTAAG TCATGGTGTA GTTGGGGCCT GTGGCCAGAG 
ATAAGGAAAT GTAAGGAAGC AGTAAGCTGC TCAAGATTGG CCGGGGCGCC ACGAGGCCTG 
TATAATTCTG TGGATAGCTG GATGATCGTG CCCAACATCA AACAGAACCA TTACACAGTC 
CACGGACTGC AGAGTGGAAC ACGCTACATC TTCATTGTTA AAGCCATAAA CCAAGCAGGC 
AGCCGGAACA GTGAACCTAC CCGACTCAAA ACAAACAGCC AACCCTTTAA ACTGGATCCC 
AAAATGACTC ACAAGAAGTT GAAGATTTCC AATGATGGAT TGCAGATGGA GAAGGAAGAA 
AGCTCTCTGA AGAAGAGCCA TACTCCAGAG AGATTTAGTG GCACTGGGTG CTATGGGGCA 
GCAGGAAATA TATTCATTGA CAGCGGTTGC CACTACTGGG AGGTGGTCAT GGGGTCCTCC 
ACATGGTATG CAGTTGGCGT TGCCTATAAA TCAGCTCCCA AGAATGAATG GATTGGCAAG 
AACGCCTCCT CGTGGGTCTT CTCGCGATGC AATAGTAACT TCGTAGTGAG ACATAACAAC 
AAGGAAATGC TGGTGGACGT GCCTCCACAG TTGAAGCGCC TGGGTGTCCT TCTGGATTAT 
GACAACAACA TGCTGTCTTT CTATGACCCA GCTAACTCTC TCCATCTTCA TACTTTTGAT 
GTGACCTTCA TTCTTCCCGT TTGTCCAACA TTCACAATTT GGAACAAATC CCTAATGATC 
CTGTCTGGCT TGCCTGCCCC AGATTTTATT GATTACCCTG AGCGGCAGGA ATGCAACTGC 
AGGCCTCAAG AATCCCCTTA TGTTTCTGGG ATGAAAGCTT GCCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024844144.1
CDS	126..2273
Translation	METLESELTCPICLELFEDPLLLPCAHSLCFSCAHRILVSSCSSGESIEPITAFQCPTCRYVISLNHRGLDGLKRNVTLQNIIDRFQKASVSGPNSPSESRRERTYRPSSAMSSERIACQFCEQDPPRDAVKTCITCEVSYCDRCLRATHPNKKPFTSHRLVEPVPDTHLRGITCLDHENEKVNMYCVSDDQLICALCKLVGRHRDHQVASLNDRFEKLKQTLEMNLTNLVKRNSELENQMAKLIQICQQVEVNTAMHEAKLMEECDELVEIIQQRKQMIAVKIKETKVMKLRKLAQQVANCRQCLERSTVLINQAEHILKENDQARFLQSAKNIAERVAMATASSQVLIPDINFNDAFENFALDFSREKKLLEGLDYLTAPNPPSIREELCTASHDTITVHWISDDEFSISSYELQYTIFTGQANFISKSWCSWGLWPEIRKCKEAVSCSRLAGAPRGLYNSVDSWMIVPNIKQNHYTVHGLQSGTRYIFIVKAINQAGSRNSEPTRLKTNSQPFKLDPKMTHKKLKISNDGLQMEKEESSLKKSHTPERFSGTGCYGAAGNIFIDSGCHYWEVVMGSSTWYAVGVAYKSAPKNEWIGKNASSWVFSRCNSNFVVRHNNKEMLVDVPPQLKRLGVLLDYDNNMLSFYDPANSLHLHTFDVTFILPVCPTFTIWNKSLMILSGLPAPDFIDYPERQECNCRPQESPYVSGMKACH

Target ORF information:

RefSeq Version	XM_024988376.1
Organism	Bos taurus(cattle)
Definition	Bos taurus midline 2 (MID2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024988376.1

ORF Insert Sequence:

ATGGAAACAC TGGAGTCTGA ATTGACCTGT CCAATCTGCC TAGAGTTGTT TGAAGACCCC 
CTTCTGCTCC CTTGTGCTCA CAGCCTCTGC TTCAGCTGTG CCCATCGCAT CTTGGTCTCG 
AGCTGCAGCT CCGGTGAATC CATTGAACCC ATTACTGCTT TCCAGTGTCC TACGTGCAGG 
TATGTTATCT CACTGAACCA CCGGGGCCTG GATGGCCTCA AGAGAAATGT GACTCTGCAG 
AACATTATTG ACCGCTTCCA GAAGGCTTCC GTCAGTGGAC CCAACTCCCC AAGTGAGAGC 
CGCCGAGAGA GGACTTACAG GCCCAGCAGC GCCATGTCTA GTGAGCGAAT TGCGTGCCAA 
TTCTGTGAGC AGGACCCGCC AAGGGATGCA GTGAAAACGT GCATCACTTG TGAGGTCTCT 
TACTGTGACC GTTGCCTGCG GGCTACACAC CCCAACAAGA AACCTTTCAC CAGCCACCGC 
CTGGTGGAAC CAGTGCCGGA CACGCATCTT CGAGGGATCA CCTGCCTGGA CCATGAAAAT 
GAGAAAGTGA ATATGTACTG TGTTTCTGAT GACCAATTGA TCTGTGCCTT ATGCAAACTG 
GTGGGGCGTC ACCGAGACCA TCAGGTCGCA TCCCTGAATG ATCGGTTTGA GAAGCTCAAG 
CAAACTCTGG AGATGAACCT CACCAACCTG GTTAAGCGCA ACAGTGAACT AGAAAATCAA 
ATGGCCAAGC TAATACAGAT CTGCCAGCAG GTTGAGGTGA ATACCGCTAT GCATGAGGCA 
AAACTTATGG AAGAATGTGA CGAATTGGTA GAGATCATCC AGCAGAGGAA GCAAATGATT 
GCTGTCAAAA TCAAAGAGAC AAAGGTTATG AAACTGAGAA AATTGGCACA GCAGGTTGCT 
AATTGCCGCC AGTGTCTTGA ACGGTCAACA GTCCTCATCA ACCAAGCTGA GCATATCCTG 
AAAGAAAATG ACCAGGCACG GTTTCTCCAG TCTGCAAAAA ATATCGCTGA GAGGGTCGCT 
ATGGCAACTG CATCTTCTCA AGTTCTGATT CCAGACATCA ATTTTAATGA TGCCTTTGAA 
AACTTTGCTT TAGATTTTTC CAGAGAAAAG AAACTGCTGG AGGGGCTAGA TTATTTAACA 
GCCCCCAACC CACCATCTAT CCGAGAGGAA CTCTGTACTG CCTCCCATGA CACCATTACG 
GTCCACTGGA TCTCGGATGA TGAGTTCAGC ATCAGCTCCT ATGAGCTTCA GTACACCATA 
TTCACTGGCC AGGCTAACTT CATCAGTAAG TCATGGTGTA GTTGGGGCCT GTGGCCAGAG 
ATAAGGAAAT GTAAGGAAGC AGTAAGCTGC TCAAGATTGG CCGGGGCGCC ACGAGGCCTG 
TATAATTCTG TGGATAGCTG GATGATCGTG CCCAACATCA AACAGAACCA TTACACAGTC 
CACGGACTGC AGAGTGGAAC ACGCTACATC TTCATTGTTA AAGCCATAAA CCAAGCAGGC 
AGCCGGAACA GTGAACCTAC CCGACTCAAA ACAAACAGCC AACCCTTTAA ACTGGATCCC 
AAAATGACTC ACAAGAAGTT GAAGATTTCC AATGATGGAT TGCAGATGGA GAAGGAAGAA 
AGCTCTCTGA AGAAGAGCCA TACTCCAGAG AGATTTAGTG GCACTGGGTG CTATGGGGCA 
GCAGGAAATA TATTCATTGA CAGCGGTTGC CACTACTGGG AGGTGGTCAT GGGGTCCTCC 
ACATGGTATG CAGTTGGCGT TGCCTATAAA TCAGCTCCCA AGAATGAATG GATTGGCAAG 
AACGCCTCCT CGTGGGTCTT CTCGCGATGC AATAGTAACT TCGTAGTGAG ACATAACAAC 
AAGGAAATGC TGGTGGACGT GCCTCCACAG TTGAAGCGCC TGGGTGTCCT TCTGGATTAT 
GACAACAACA TGCTGTCTTT CTATGACCCA GCTAACTCTC TCCATCTTCA TACTTTTGAT 
GTGACCTTCA TTCTTCCCGT TTGTCCAACA TTCACAATTT GGAACAAATC CCTAATGATC 
CTGTCTGGCT TGCCTGCCCC AGATTTTATT GATTACCCTG AGCGGCAGGA ATGCAACTGC 
AGGCCTCAAG AATCCCCTTA TGTTTCTGGG ATGAAAGCTT GCCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MID2 ( XM_024988374.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024844142.1 Bos taurus midline 2 (MID2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa238552
Clone ID Related Accession (Same CDS sequence)	XM_024988376.1 , XM_024988374.1 , XM_024988375.1
Accession Version	XM_024988374.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2148bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	probable E3 ubiquitin-protein ligase MID2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAACAC TGGAGTCTGA ATTGACCTGT CCAATCTGCC TAGAGTTGTT TGAAGACCCC 
CTTCTGCTCC CTTGTGCTCA CAGCCTCTGC TTCAGCTGTG CCCATCGCAT CTTGGTCTCG 
AGCTGCAGCT CCGGTGAATC CATTGAACCC ATTACTGCTT TCCAGTGTCC TACGTGCAGG 
TATGTTATCT CACTGAACCA CCGGGGCCTG GATGGCCTCA AGAGAAATGT GACTCTGCAG 
AACATTATTG ACCGCTTCCA GAAGGCTTCC GTCAGTGGAC CCAACTCCCC AAGTGAGAGC 
CGCCGAGAGA GGACTTACAG GCCCAGCAGC GCCATGTCTA GTGAGCGAAT TGCGTGCCAA 
TTCTGTGAGC AGGACCCGCC AAGGGATGCA GTGAAAACGT GCATCACTTG TGAGGTCTCT 
TACTGTGACC GTTGCCTGCG GGCTACACAC CCCAACAAGA AACCTTTCAC CAGCCACCGC 
CTGGTGGAAC CAGTGCCGGA CACGCATCTT CGAGGGATCA CCTGCCTGGA CCATGAAAAT 
GAGAAAGTGA ATATGTACTG TGTTTCTGAT GACCAATTGA TCTGTGCCTT ATGCAAACTG 
GTGGGGCGTC ACCGAGACCA TCAGGTCGCA TCCCTGAATG ATCGGTTTGA GAAGCTCAAG 
CAAACTCTGG AGATGAACCT CACCAACCTG GTTAAGCGCA ACAGTGAACT AGAAAATCAA 
ATGGCCAAGC TAATACAGAT CTGCCAGCAG GTTGAGGTGA ATACCGCTAT GCATGAGGCA 
AAACTTATGG AAGAATGTGA CGAATTGGTA GAGATCATCC AGCAGAGGAA GCAAATGATT 
GCTGTCAAAA TCAAAGAGAC AAAGGTTATG AAACTGAGAA AATTGGCACA GCAGGTTGCT 
AATTGCCGCC AGTGTCTTGA ACGGTCAACA GTCCTCATCA ACCAAGCTGA GCATATCCTG 
AAAGAAAATG ACCAGGCACG GTTTCTCCAG TCTGCAAAAA ATATCGCTGA GAGGGTCGCT 
ATGGCAACTG CATCTTCTCA AGTTCTGATT CCAGACATCA ATTTTAATGA TGCCTTTGAA 
AACTTTGCTT TAGATTTTTC CAGAGAAAAG AAACTGCTGG AGGGGCTAGA TTATTTAACA 
GCCCCCAACC CACCATCTAT CCGAGAGGAA CTCTGTACTG CCTCCCATGA CACCATTACG 
GTCCACTGGA TCTCGGATGA TGAGTTCAGC ATCAGCTCCT ATGAGCTTCA GTACACCATA 
TTCACTGGCC AGGCTAACTT CATCAGTAAG TCATGGTGTA GTTGGGGCCT GTGGCCAGAG 
ATAAGGAAAT GTAAGGAAGC AGTAAGCTGC TCAAGATTGG CCGGGGCGCC ACGAGGCCTG 
TATAATTCTG TGGATAGCTG GATGATCGTG CCCAACATCA AACAGAACCA TTACACAGTC 
CACGGACTGC AGAGTGGAAC ACGCTACATC TTCATTGTTA AAGCCATAAA CCAAGCAGGC 
AGCCGGAACA GTGAACCTAC CCGACTCAAA ACAAACAGCC AACCCTTTAA ACTGGATCCC 
AAAATGACTC ACAAGAAGTT GAAGATTTCC AATGATGGAT TGCAGATGGA GAAGGAAGAA 
AGCTCTCTGA AGAAGAGCCA TACTCCAGAG AGATTTAGTG GCACTGGGTG CTATGGGGCA 
GCAGGAAATA TATTCATTGA CAGCGGTTGC CACTACTGGG AGGTGGTCAT GGGGTCCTCC 
ACATGGTATG CAGTTGGCGT TGCCTATAAA TCAGCTCCCA AGAATGAATG GATTGGCAAG 
AACGCCTCCT CGTGGGTCTT CTCGCGATGC AATAGTAACT TCGTAGTGAG ACATAACAAC 
AAGGAAATGC TGGTGGACGT GCCTCCACAG TTGAAGCGCC TGGGTGTCCT TCTGGATTAT 
GACAACAACA TGCTGTCTTT CTATGACCCA GCTAACTCTC TCCATCTTCA TACTTTTGAT 
GTGACCTTCA TTCTTCCCGT TTGTCCAACA TTCACAATTT GGAACAAATC CCTAATGATC 
CTGTCTGGCT TGCCTGCCCC AGATTTTATT GATTACCCTG AGCGGCAGGA ATGCAACTGC 
AGGCCTCAAG AATCCCCTTA TGTTTCTGGG ATGAAAGCTT GCCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024844142.1
CDS	359..2506
Translation	METLESELTCPICLELFEDPLLLPCAHSLCFSCAHRILVSSCSSGESIEPITAFQCPTCRYVISLNHRGLDGLKRNVTLQNIIDRFQKASVSGPNSPSESRRERTYRPSSAMSSERIACQFCEQDPPRDAVKTCITCEVSYCDRCLRATHPNKKPFTSHRLVEPVPDTHLRGITCLDHENEKVNMYCVSDDQLICALCKLVGRHRDHQVASLNDRFEKLKQTLEMNLTNLVKRNSELENQMAKLIQICQQVEVNTAMHEAKLMEECDELVEIIQQRKQMIAVKIKETKVMKLRKLAQQVANCRQCLERSTVLINQAEHILKENDQARFLQSAKNIAERVAMATASSQVLIPDINFNDAFENFALDFSREKKLLEGLDYLTAPNPPSIREELCTASHDTITVHWISDDEFSISSYELQYTIFTGQANFISKSWCSWGLWPEIRKCKEAVSCSRLAGAPRGLYNSVDSWMIVPNIKQNHYTVHGLQSGTRYIFIVKAINQAGSRNSEPTRLKTNSQPFKLDPKMTHKKLKISNDGLQMEKEESSLKKSHTPERFSGTGCYGAAGNIFIDSGCHYWEVVMGSSTWYAVGVAYKSAPKNEWIGKNASSWVFSRCNSNFVVRHNNKEMLVDVPPQLKRLGVLLDYDNNMLSFYDPANSLHLHTFDVTFILPVCPTFTIWNKSLMILSGLPAPDFIDYPERQECNCRPQESPYVSGMKACH

Target ORF information:

RefSeq Version	XM_024988374.1
Organism	Bos taurus(cattle)
Definition	Bos taurus midline 2 (MID2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024988374.1

ORF Insert Sequence:

ATGGAAACAC TGGAGTCTGA ATTGACCTGT CCAATCTGCC TAGAGTTGTT TGAAGACCCC 
CTTCTGCTCC CTTGTGCTCA CAGCCTCTGC TTCAGCTGTG CCCATCGCAT CTTGGTCTCG 
AGCTGCAGCT CCGGTGAATC CATTGAACCC ATTACTGCTT TCCAGTGTCC TACGTGCAGG 
TATGTTATCT CACTGAACCA CCGGGGCCTG GATGGCCTCA AGAGAAATGT GACTCTGCAG 
AACATTATTG ACCGCTTCCA GAAGGCTTCC GTCAGTGGAC CCAACTCCCC AAGTGAGAGC 
CGCCGAGAGA GGACTTACAG GCCCAGCAGC GCCATGTCTA GTGAGCGAAT TGCGTGCCAA 
TTCTGTGAGC AGGACCCGCC AAGGGATGCA GTGAAAACGT GCATCACTTG TGAGGTCTCT 
TACTGTGACC GTTGCCTGCG GGCTACACAC CCCAACAAGA AACCTTTCAC CAGCCACCGC 
CTGGTGGAAC CAGTGCCGGA CACGCATCTT CGAGGGATCA CCTGCCTGGA CCATGAAAAT 
GAGAAAGTGA ATATGTACTG TGTTTCTGAT GACCAATTGA TCTGTGCCTT ATGCAAACTG 
GTGGGGCGTC ACCGAGACCA TCAGGTCGCA TCCCTGAATG ATCGGTTTGA GAAGCTCAAG 
CAAACTCTGG AGATGAACCT CACCAACCTG GTTAAGCGCA ACAGTGAACT AGAAAATCAA 
ATGGCCAAGC TAATACAGAT CTGCCAGCAG GTTGAGGTGA ATACCGCTAT GCATGAGGCA 
AAACTTATGG AAGAATGTGA CGAATTGGTA GAGATCATCC AGCAGAGGAA GCAAATGATT 
GCTGTCAAAA TCAAAGAGAC AAAGGTTATG AAACTGAGAA AATTGGCACA GCAGGTTGCT 
AATTGCCGCC AGTGTCTTGA ACGGTCAACA GTCCTCATCA ACCAAGCTGA GCATATCCTG 
AAAGAAAATG ACCAGGCACG GTTTCTCCAG TCTGCAAAAA ATATCGCTGA GAGGGTCGCT 
ATGGCAACTG CATCTTCTCA AGTTCTGATT CCAGACATCA ATTTTAATGA TGCCTTTGAA 
AACTTTGCTT TAGATTTTTC CAGAGAAAAG AAACTGCTGG AGGGGCTAGA TTATTTAACA 
GCCCCCAACC CACCATCTAT CCGAGAGGAA CTCTGTACTG CCTCCCATGA CACCATTACG 
GTCCACTGGA TCTCGGATGA TGAGTTCAGC ATCAGCTCCT ATGAGCTTCA GTACACCATA 
TTCACTGGCC AGGCTAACTT CATCAGTAAG TCATGGTGTA GTTGGGGCCT GTGGCCAGAG 
ATAAGGAAAT GTAAGGAAGC AGTAAGCTGC TCAAGATTGG CCGGGGCGCC ACGAGGCCTG 
TATAATTCTG TGGATAGCTG GATGATCGTG CCCAACATCA AACAGAACCA TTACACAGTC 
CACGGACTGC AGAGTGGAAC ACGCTACATC TTCATTGTTA AAGCCATAAA CCAAGCAGGC 
AGCCGGAACA GTGAACCTAC CCGACTCAAA ACAAACAGCC AACCCTTTAA ACTGGATCCC 
AAAATGACTC ACAAGAAGTT GAAGATTTCC AATGATGGAT TGCAGATGGA GAAGGAAGAA 
AGCTCTCTGA AGAAGAGCCA TACTCCAGAG AGATTTAGTG GCACTGGGTG CTATGGGGCA 
GCAGGAAATA TATTCATTGA CAGCGGTTGC CACTACTGGG AGGTGGTCAT GGGGTCCTCC 
ACATGGTATG CAGTTGGCGT TGCCTATAAA TCAGCTCCCA AGAATGAATG GATTGGCAAG 
AACGCCTCCT CGTGGGTCTT CTCGCGATGC AATAGTAACT TCGTAGTGAG ACATAACAAC 
AAGGAAATGC TGGTGGACGT GCCTCCACAG TTGAAGCGCC TGGGTGTCCT TCTGGATTAT 
GACAACAACA TGCTGTCTTT CTATGACCCA GCTAACTCTC TCCATCTTCA TACTTTTGAT 
GTGACCTTCA TTCTTCCCGT TTGTCCAACA TTCACAATTT GGAACAAATC CCTAATGATC 
CTGTCTGGCT TGCCTGCCCC AGATTTTATT GATTACCCTG AGCGGCAGGA ATGCAACTGC 
AGGCCTCAAG AATCCCCTTA TGTTTCTGGG ATGAAAGCTT GCCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MID2 ( XM_024988375.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024844143.1 Bos taurus midline 2 (MID2), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBa238552
Clone ID Related Accession (Same CDS sequence)	XM_024988376.1 , XM_024988374.1 , XM_024988375.1
Accession Version	XM_024988375.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2148bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	probable E3 ubiquitin-protein ligase MID2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAACAC TGGAGTCTGA ATTGACCTGT CCAATCTGCC TAGAGTTGTT TGAAGACCCC 
CTTCTGCTCC CTTGTGCTCA CAGCCTCTGC TTCAGCTGTG CCCATCGCAT CTTGGTCTCG 
AGCTGCAGCT CCGGTGAATC CATTGAACCC ATTACTGCTT TCCAGTGTCC TACGTGCAGG 
TATGTTATCT CACTGAACCA CCGGGGCCTG GATGGCCTCA AGAGAAATGT GACTCTGCAG 
AACATTATTG ACCGCTTCCA GAAGGCTTCC GTCAGTGGAC CCAACTCCCC AAGTGAGAGC 
CGCCGAGAGA GGACTTACAG GCCCAGCAGC GCCATGTCTA GTGAGCGAAT TGCGTGCCAA 
TTCTGTGAGC AGGACCCGCC AAGGGATGCA GTGAAAACGT GCATCACTTG TGAGGTCTCT 
TACTGTGACC GTTGCCTGCG GGCTACACAC CCCAACAAGA AACCTTTCAC CAGCCACCGC 
CTGGTGGAAC CAGTGCCGGA CACGCATCTT CGAGGGATCA CCTGCCTGGA CCATGAAAAT 
GAGAAAGTGA ATATGTACTG TGTTTCTGAT GACCAATTGA TCTGTGCCTT ATGCAAACTG 
GTGGGGCGTC ACCGAGACCA TCAGGTCGCA TCCCTGAATG ATCGGTTTGA GAAGCTCAAG 
CAAACTCTGG AGATGAACCT CACCAACCTG GTTAAGCGCA ACAGTGAACT AGAAAATCAA 
ATGGCCAAGC TAATACAGAT CTGCCAGCAG GTTGAGGTGA ATACCGCTAT GCATGAGGCA 
AAACTTATGG AAGAATGTGA CGAATTGGTA GAGATCATCC AGCAGAGGAA GCAAATGATT 
GCTGTCAAAA TCAAAGAGAC AAAGGTTATG AAACTGAGAA AATTGGCACA GCAGGTTGCT 
AATTGCCGCC AGTGTCTTGA ACGGTCAACA GTCCTCATCA ACCAAGCTGA GCATATCCTG 
AAAGAAAATG ACCAGGCACG GTTTCTCCAG TCTGCAAAAA ATATCGCTGA GAGGGTCGCT 
ATGGCAACTG CATCTTCTCA AGTTCTGATT CCAGACATCA ATTTTAATGA TGCCTTTGAA 
AACTTTGCTT TAGATTTTTC CAGAGAAAAG AAACTGCTGG AGGGGCTAGA TTATTTAACA 
GCCCCCAACC CACCATCTAT CCGAGAGGAA CTCTGTACTG CCTCCCATGA CACCATTACG 
GTCCACTGGA TCTCGGATGA TGAGTTCAGC ATCAGCTCCT ATGAGCTTCA GTACACCATA 
TTCACTGGCC AGGCTAACTT CATCAGTAAG TCATGGTGTA GTTGGGGCCT GTGGCCAGAG 
ATAAGGAAAT GTAAGGAAGC AGTAAGCTGC TCAAGATTGG CCGGGGCGCC ACGAGGCCTG 
TATAATTCTG TGGATAGCTG GATGATCGTG CCCAACATCA AACAGAACCA TTACACAGTC 
CACGGACTGC AGAGTGGAAC ACGCTACATC TTCATTGTTA AAGCCATAAA CCAAGCAGGC 
AGCCGGAACA GTGAACCTAC CCGACTCAAA ACAAACAGCC AACCCTTTAA ACTGGATCCC 
AAAATGACTC ACAAGAAGTT GAAGATTTCC AATGATGGAT TGCAGATGGA GAAGGAAGAA 
AGCTCTCTGA AGAAGAGCCA TACTCCAGAG AGATTTAGTG GCACTGGGTG CTATGGGGCA 
GCAGGAAATA TATTCATTGA CAGCGGTTGC CACTACTGGG AGGTGGTCAT GGGGTCCTCC 
ACATGGTATG CAGTTGGCGT TGCCTATAAA TCAGCTCCCA AGAATGAATG GATTGGCAAG 
AACGCCTCCT CGTGGGTCTT CTCGCGATGC AATAGTAACT TCGTAGTGAG ACATAACAAC 
AAGGAAATGC TGGTGGACGT GCCTCCACAG TTGAAGCGCC TGGGTGTCCT TCTGGATTAT 
GACAACAACA TGCTGTCTTT CTATGACCCA GCTAACTCTC TCCATCTTCA TACTTTTGAT 
GTGACCTTCA TTCTTCCCGT TTGTCCAACA TTCACAATTT GGAACAAATC CCTAATGATC 
CTGTCTGGCT TGCCTGCCCC AGATTTTATT GATTACCCTG AGCGGCAGGA ATGCAACTGC 
AGGCCTCAAG AATCCCCTTA TGTTTCTGGG ATGAAAGCTT GCCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024844143.1
CDS	201..2348
Translation	METLESELTCPICLELFEDPLLLPCAHSLCFSCAHRILVSSCSSGESIEPITAFQCPTCRYVISLNHRGLDGLKRNVTLQNIIDRFQKASVSGPNSPSESRRERTYRPSSAMSSERIACQFCEQDPPRDAVKTCITCEVSYCDRCLRATHPNKKPFTSHRLVEPVPDTHLRGITCLDHENEKVNMYCVSDDQLICALCKLVGRHRDHQVASLNDRFEKLKQTLEMNLTNLVKRNSELENQMAKLIQICQQVEVNTAMHEAKLMEECDELVEIIQQRKQMIAVKIKETKVMKLRKLAQQVANCRQCLERSTVLINQAEHILKENDQARFLQSAKNIAERVAMATASSQVLIPDINFNDAFENFALDFSREKKLLEGLDYLTAPNPPSIREELCTASHDTITVHWISDDEFSISSYELQYTIFTGQANFISKSWCSWGLWPEIRKCKEAVSCSRLAGAPRGLYNSVDSWMIVPNIKQNHYTVHGLQSGTRYIFIVKAINQAGSRNSEPTRLKTNSQPFKLDPKMTHKKLKISNDGLQMEKEESSLKKSHTPERFSGTGCYGAAGNIFIDSGCHYWEVVMGSSTWYAVGVAYKSAPKNEWIGKNASSWVFSRCNSNFVVRHNNKEMLVDVPPQLKRLGVLLDYDNNMLSFYDPANSLHLHTFDVTFILPVCPTFTIWNKSLMILSGLPAPDFIDYPERQECNCRPQESPYVSGMKACH

Target ORF information:

RefSeq Version	XM_024988375.1
Organism	Bos taurus(cattle)
Definition	Bos taurus midline 2 (MID2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024988375.1

ORF Insert Sequence:

ATGGAAACAC TGGAGTCTGA ATTGACCTGT CCAATCTGCC TAGAGTTGTT TGAAGACCCC 
CTTCTGCTCC CTTGTGCTCA CAGCCTCTGC TTCAGCTGTG CCCATCGCAT CTTGGTCTCG 
AGCTGCAGCT CCGGTGAATC CATTGAACCC ATTACTGCTT TCCAGTGTCC TACGTGCAGG 
TATGTTATCT CACTGAACCA CCGGGGCCTG GATGGCCTCA AGAGAAATGT GACTCTGCAG 
AACATTATTG ACCGCTTCCA GAAGGCTTCC GTCAGTGGAC CCAACTCCCC AAGTGAGAGC 
CGCCGAGAGA GGACTTACAG GCCCAGCAGC GCCATGTCTA GTGAGCGAAT TGCGTGCCAA 
TTCTGTGAGC AGGACCCGCC AAGGGATGCA GTGAAAACGT GCATCACTTG TGAGGTCTCT 
TACTGTGACC GTTGCCTGCG GGCTACACAC CCCAACAAGA AACCTTTCAC CAGCCACCGC 
CTGGTGGAAC CAGTGCCGGA CACGCATCTT CGAGGGATCA CCTGCCTGGA CCATGAAAAT 
GAGAAAGTGA ATATGTACTG TGTTTCTGAT GACCAATTGA TCTGTGCCTT ATGCAAACTG 
GTGGGGCGTC ACCGAGACCA TCAGGTCGCA TCCCTGAATG ATCGGTTTGA GAAGCTCAAG 
CAAACTCTGG AGATGAACCT CACCAACCTG GTTAAGCGCA ACAGTGAACT AGAAAATCAA 
ATGGCCAAGC TAATACAGAT CTGCCAGCAG GTTGAGGTGA ATACCGCTAT GCATGAGGCA 
AAACTTATGG AAGAATGTGA CGAATTGGTA GAGATCATCC AGCAGAGGAA GCAAATGATT 
GCTGTCAAAA TCAAAGAGAC AAAGGTTATG AAACTGAGAA AATTGGCACA GCAGGTTGCT 
AATTGCCGCC AGTGTCTTGA ACGGTCAACA GTCCTCATCA ACCAAGCTGA GCATATCCTG 
AAAGAAAATG ACCAGGCACG GTTTCTCCAG TCTGCAAAAA ATATCGCTGA GAGGGTCGCT 
ATGGCAACTG CATCTTCTCA AGTTCTGATT CCAGACATCA ATTTTAATGA TGCCTTTGAA 
AACTTTGCTT TAGATTTTTC CAGAGAAAAG AAACTGCTGG AGGGGCTAGA TTATTTAACA 
GCCCCCAACC CACCATCTAT CCGAGAGGAA CTCTGTACTG CCTCCCATGA CACCATTACG 
GTCCACTGGA TCTCGGATGA TGAGTTCAGC ATCAGCTCCT ATGAGCTTCA GTACACCATA 
TTCACTGGCC AGGCTAACTT CATCAGTAAG TCATGGTGTA GTTGGGGCCT GTGGCCAGAG 
ATAAGGAAAT GTAAGGAAGC AGTAAGCTGC TCAAGATTGG CCGGGGCGCC ACGAGGCCTG 
TATAATTCTG TGGATAGCTG GATGATCGTG CCCAACATCA AACAGAACCA TTACACAGTC 
CACGGACTGC AGAGTGGAAC ACGCTACATC TTCATTGTTA AAGCCATAAA CCAAGCAGGC 
AGCCGGAACA GTGAACCTAC CCGACTCAAA ACAAACAGCC AACCCTTTAA ACTGGATCCC 
AAAATGACTC ACAAGAAGTT GAAGATTTCC AATGATGGAT TGCAGATGGA GAAGGAAGAA 
AGCTCTCTGA AGAAGAGCCA TACTCCAGAG AGATTTAGTG GCACTGGGTG CTATGGGGCA 
GCAGGAAATA TATTCATTGA CAGCGGTTGC CACTACTGGG AGGTGGTCAT GGGGTCCTCC 
ACATGGTATG CAGTTGGCGT TGCCTATAAA TCAGCTCCCA AGAATGAATG GATTGGCAAG 
AACGCCTCCT CGTGGGTCTT CTCGCGATGC AATAGTAACT TCGTAGTGAG ACATAACAAC 
AAGGAAATGC TGGTGGACGT GCCTCCACAG TTGAAGCGCC TGGGTGTCCT TCTGGATTAT 
GACAACAACA TGCTGTCTTT CTATGACCCA GCTAACTCTC TCCATCTTCA TACTTTTGAT 
GTGACCTTCA TTCTTCCCGT TTGTCCAACA TTCACAATTT GGAACAAATC CCTAATGATC 
CTGTCTGGCT TGCCTGCCCC AGATTTTATT GATTACCCTG AGCGGCAGGA ATGCAACTGC 
AGGCCTCAAG AATCCCCTTA TGTTTCTGGG ATGAAAGCTT GCCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	MID2
Entrez Gene ID	616821
Full Name	midline 2
Gene Type	protein-coding
Organism	Bos taurus(cattle)

Bos taurus (cattle)	LOC616821	XP_002699930.2
Danio rerio (zebrafish)	mid2	XP_001340198.5

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

MID2 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

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Target ORF information:

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