mid2 gene cDNA ORF clone, Danio rerio(zebrafish)

mRNA	Protein	Name
XM_009291073.2	XP_009289348.1	probable E3 ubiquitin-protein ligase MID2 isoform X1
XM_009291074.2	XP_009289349.1	probable E3 ubiquitin-protein ligase MID2 isoform X1
XM_009291076.2	XP_009289351.1	probable E3 ubiquitin-protein ligase MID2 isoform X2
XM_009291075.2	XP_009289350.1	probable E3 ubiquitin-protein ligase MID2 isoform X1
XM_009291077.2	XP_009289352.1	probable E3 ubiquitin-protein ligase MID2 isoform X2
XM_009291074.3	XP_009289349.1	probable E3 ubiquitin-protein ligase MID2 isoform X1
XM_009291073.3	XP_009289348.1	probable E3 ubiquitin-protein ligase MID2 isoform X1
XM_021481165.1	XP_021336840.1	probable E3 ubiquitin-protein ligase MID2 isoform X1
XM_009291077.3	XP_009289352.1	probable E3 ubiquitin-protein ligase MID2 isoform X2
XM_009291075.3	XP_009289350.1	probable E3 ubiquitin-protein ligase MID2 isoform X1
XM_009291076.3	XP_009289351.1	probable E3 ubiquitin-protein ligase MID2 isoform X2

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa47435	XM_009291073.3 Latest version!	Danio rerio midline 2 (mid2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
ODa47435	XM_009291074.3 Latest version!	Danio rerio midline 2 (mid2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
ODa47435	XM_021481165.1 Latest version!	Danio rerio midline 2 (mid2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
ODa47435	XM_009291075.3 Latest version!	Danio rerio midline 2 (mid2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
ODa47436	XM_009291077.3 Latest version!	Danio rerio midline 2 (mid2), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
ODa47436	XM_009291076.3 Latest version!	Danio rerio midline 2 (mid2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
View Old Accession Versions >>
ODa47435	XM_009291074.2	Danio rerio midline 2 (mid2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
ODa47435	XM_009291073.2	Danio rerio midline 2 (mid2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
ODa47436	XM_009291077.2	Danio rerio midline 2 (mid2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
ODa47435	XM_009291075.2	Danio rerio midline 2 (mid2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
ODa47436	XM_009291076.2	Danio rerio midline 2 (mid2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
Hide Old Accession Versions >>

mid2 ( XM_009291073.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009289348.1 Danio rerio midline 2 (mid2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa47435
Clone ID Related Accession (Same CDS sequence)	XM_009291073.2 , XM_009291074.2 , XM_009291075.2 , XM_009291074.3 , XM_009291073.3 , XM_021481165.1 , XM_009291075.3
Accession Version	XM_009291073.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2157bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	probable E3 ubiquitin-protein ligase MID2 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007125.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009291073.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAACCC TGGAGTCCGA GCTGACGTGC CCGATCTGCC TGGAACTGTT TGAGGACCCG 
CTCCTGCTCC CCTGCGCCCA CAGCCTGTGC TTCAACTGCG CCCACCGCAT CTTGGTGTCC 
CACTGCTCAA GCACCAAACC CCTCGAGTCC ATCTCTGCCT TCCAGTGCCC CACCTGCCGC 
TACGTCATCA CGCTTAACCA GCGCGGCCTG GAGGGCCTAA AGCGCAATGT GACACTGCAG 
AACATCATCG ACCGCTTCCA GAAGGCCTCT GTCAGCGGGC CTAATTCTCC AAACGAGAGT 
CGCAGGCCCC GGCCCTACAG CGCCACGCTA GGCGGGAGCG CGAGGGGCAG CCCCGCCAGC 
AGCGCCATGT CCCTCGAGCG TGTCGGGTGT CAGTTCTGTG ACCAGGAGCC TCCGCGCGAG 
GCGGTGAAGA CATGTGTGAC GTGCGAGGTG TCATACTGCG ACCGCTGCCT GAGGGCCACA 
CACCCCAATA AGAAGCCCTT CACCAGCCAT CGTCTGGTGG AGCCAGTGGC GGATGCTCAG 
CTGCGTGGAC TAGCCTGTTT GGAGCATGAA AACGAGAAGG TCAACATGTA CTGTGTGGTT 
GACGATCAGC TCATCTGCGC CCTTTGCAAG TTGGTGGGAC GCCACCGTGA ACATCAGGTC 
ACCTCGCTGA GCGATCGCTT CGACAAGCTG AAGGCTTTTC AAGTGAGCCC TGCCAGAGTT 
TCTGAAGCGG TTATGGAGGC ATCAAAGACA AGCGCCGCGC AAACCTTGGA GAATAACTTA 
ACCAATCTGG TGAAGAGGAA CAGTGAGCTG GAGAACCAGA TGGCCAAACT CATCCAGATT 
TGTCAACAGG TGGAGGTGAA CACAGCCATG CATGAAGCCA AGCTGGTGGA GGAGTGTGAT 
GAATTGGTGG AGATAATTCG CCAGAGGAAA CAGGTCATTG CTGTCAAGAT CAAAGAGTCC 
AAGGTGATGA AGCTGAGGAA GTTGGCTCAG CAGATTGCGA ACTGCAGACA GTGTCTGGAG 
CGCTCGAGCG CCCTCATCAC ACAGGCGGAG CACAGCCTGA AGGAGAACGA CCACACCCGA 
TTCCTCCAGA CGGCCAGGAA TGTGGCGGAG AGAGTTGCCA TGGCCACCGC CTCCTCCCAG 
GTCCTCATCC CAGACGTCAA TCTGAACGAG GCCTTTGACA ATTTTGCCCT TGATTTCTCC 
AGAGAAAAGA AAATCCTGGA AGGACTGGAT TATCTGACAG CTCCCAACCC TCCCTCGATA 
CGCGAAGAGC TCTGCACGGC CTCTCACGAC ACCATTACAG TGCACTGGAC ATCTGAAGAT 
GAATTCAGCG TCACGTCTTA TGAGCTTCAG TACACCATCT ACACTGGACA GACCAACTTC 
ATCAGTCTAT ATAACTCTGT GGATAGTTGG ATGATTGTAC CCAATATCAA GCAGAATCAC 
TACACAGTCC ACGGCCTGCA GAGCGGGACT CGTTACATCT TTTTCGTCAA GGCCATCAAC 
CAGGCAGGAA GCAGAAACAG TGAACCCGCT CGCCTCAAAA CCAACAGTCA GCCTTTCAAA 
CTAGACCCCA AAACGGCCCA TAAGAAACTG CGGCTCTCGA ATGACTGTCT GACAATGGAG 
AAGGATGAAA GCTCGCTGAA GAAAAGCCAC ACTCCAGAAC GCTTCAGCGG AACTGGATCT 
TATGGAGCTG CTGGCAACGT CTTCATTGAC AGTGGTTGCC ATTATTGGGA AGTACTACTG 
GGAGCCTCTA CATGGTACGC TCTTGGTGTG GCCTATAAGT CAGCACCCAA AAACGAGTGG 
AGTGGTAAAA ACTCATCATC TTGGGTTTTC TCACGCTGCA ACAATAACTT CTTGGTGCGG 
CACAACAACA AGGAGTCCAT CGTGGAAGCG TCACTACAGC TGCGCAGGGT GGGAGTTCTT 
CTGGACTACG ACAACAACAC TCTGTCCTTC TATGATGCCA TGAACTCCCA GCACATATAC 
ACTTTTGACG TTTCCTTCCT GCTTCCGGTG GCTCCCACCT TCATGATCTG GAACAAGTCA 
TTGATGATCA TGTCAGGGCT GCCGGTGCCT GACTTTGTGG ACTGCAGCGA GCAGCAAGAG 
GGCATCTGCC GCCAACAGGA GTCACCCTAT GTTTCTGGAA TAAAGAGCTG CCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009289348.1
CDS	242..2398
Translation	METLESELTCPICLELFEDPLLLPCAHSLCFNCAHRILVSHCSSTKPLESISAFQCPTCRYVITLNQRGLEGLKRNVTLQNIIDRFQKASVSGPNSPNESRRPRPYSATLGGSARGSPASSAMSLERVGCQFCDQEPPREAVKTCVTCEVSYCDRCLRATHPNKKPFTSHRLVEPVADAQLRGLACLEHENEKVNMYCVVDDQLICALCKLVGRHREHQVTSLSDRFDKLKAFQVSPARVSEAVMEASKTSAAQTLENNLTNLVKRNSELENQMAKLIQICQQVEVNTAMHEAKLVEECDELVEIIRQRKQVIAVKIKESKVMKLRKLAQQIANCRQCLERSSALITQAEHSLKENDHTRFLQTARNVAERVAMATASSQVLIPDVNLNEAFDNFALDFSREKKILEGLDYLTAPNPPSIREELCTASHDTITVHWTSEDEFSVTSYELQYTIYTGQTNFISLYNSVDSWMIVPNIKQNHYTVHGLQSGTRYIFFVKAINQAGSRNSEPARLKTNSQPFKLDPKTAHKKLRLSNDCLTMEKDESSLKKSHTPERFSGTGSYGAAGNVFIDSGCHYWEVLLGASTWYALGVAYKSAPKNEWSGKNSSSWVFSRCNNNFLVRHNNKESIVEASLQLRRVGVLLDYDNNTLSFYDAMNSQHIYTFDVSFLLPVAPTFMIWNKSLMIMSGLPVPDFVDCSEQQEGICRQQESPYVSGIKSCH

Target ORF information:

RefSeq Version	XM_009291073.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio midline 2 (mid2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009291073.2

ORF Insert Sequence:

ATGGAAACCC TGGAGTCCGA GCTGACGTGC CCGATCTGCC TGGAACTGTT TGAGGACCCG 
CTCCTGCTCC CCTGCGCCCA CAGCCTGTGC TTCAACTGCG CCCACCGCAT CTTGGTGTCC 
CACTGCTCAA GCACCAAACC CCTCGAGTCC ATCTCTGCCT TCCAGTGCCC CACCTGCCGC 
TACGTCATCA CGCTTAACCA GCGCGGCCTG GAGGGCCTAA AGCGCAATGT GACACTGCAG 
AACATCATCG ACCGCTTCCA GAAGGCCTCT GTCAGCGGGC CTAATTCTCC AAACGAGAGT 
CGCAGGCCCC GGCCCTACAG CGCCACGCTA GGCGGGAGCG CGAGGGGCAG CCCCGCCAGC 
AGCGCCATGT CCCTCGAGCG TGTCGGGTGT CAGTTCTGTG ACCAGGAGCC TCCGCGCGAG 
GCGGTGAAGA CATGTGTGAC GTGCGAGGTG TCATACTGCG ACCGCTGCCT GAGGGCCACA 
CACCCCAATA AGAAGCCCTT CACCAGCCAT CGTCTGGTGG AGCCAGTGGC GGATGCTCAG 
CTGCGTGGAC TAGCCTGTTT GGAGCATGAA AACGAGAAGG TCAACATGTA CTGTGTGGTT 
GACGATCAGC TCATCTGCGC CCTTTGCAAG TTGGTGGGAC GCCACCGTGA ACATCAGGTC 
ACCTCGCTGA GCGATCGCTT CGACAAGCTG AAGGCTTTTC AAGTGAGCCC TGCCAGAGTT 
TCTGAAGCGG TTATGGAGGC ATCAAAGACA AGCGCCGCGC AAACCTTGGA GAATAACTTA 
ACCAATCTGG TGAAGAGGAA CAGTGAGCTG GAGAACCAGA TGGCCAAACT CATCCAGATT 
TGTCAACAGG TGGAGGTGAA CACAGCCATG CATGAAGCCA AGCTGGTGGA GGAGTGTGAT 
GAATTGGTGG AGATAATTCG CCAGAGGAAA CAGGTCATTG CTGTCAAGAT CAAAGAGTCC 
AAGGTGATGA AGCTGAGGAA GTTGGCTCAG CAGATTGCGA ACTGCAGACA GTGTCTGGAG 
CGCTCGAGCG CCCTCATCAC ACAGGCGGAG CACAGCCTGA AGGAGAACGA CCACACCCGA 
TTCCTCCAGA CGGCCAGGAA TGTGGCGGAG AGAGTTGCCA TGGCCACCGC CTCCTCCCAG 
GTCCTCATCC CAGACGTCAA TCTGAACGAG GCCTTTGACA ATTTTGCCCT TGATTTCTCC 
AGAGAAAAGA AAATCCTGGA AGGACTGGAT TATCTGACAG CTCCCAACCC TCCCTCGATA 
CGCGAAGAGC TCTGCACGGC CTCTCACGAC ACCATTACAG TGCACTGGAC ATCTGAAGAT 
GAATTCAGCG TCACGTCTTA TGAGCTTCAG TACACCATCT ACACTGGACA GACCAACTTC 
ATCAGTCTAT ATAACTCTGT GGATAGTTGG ATGATTGTAC CCAATATCAA GCAGAATCAC 
TACACAGTCC ACGGCCTGCA GAGCGGGACT CGTTACATCT TTTTCGTCAA GGCCATCAAC 
CAGGCAGGAA GCAGAAACAG TGAACCCGCT CGCCTCAAAA CCAACAGTCA GCCTTTCAAA 
CTAGACCCCA AAACGGCCCA TAAGAAACTG CGGCTCTCGA ATGACTGTCT GACAATGGAG 
AAGGATGAAA GCTCGCTGAA GAAAAGCCAC ACTCCAGAAC GCTTCAGCGG AACTGGATCT 
TATGGAGCTG CTGGCAACGT CTTCATTGAC AGTGGTTGCC ATTATTGGGA AGTACTACTG 
GGAGCCTCTA CATGGTACGC TCTTGGTGTG GCCTATAAGT CAGCACCCAA AAACGAGTGG 
AGTGGTAAAA ACTCATCATC TTGGGTTTTC TCACGCTGCA ACAATAACTT CTTGGTGCGG 
CACAACAACA AGGAGTCCAT CGTGGAAGCG TCACTACAGC TGCGCAGGGT GGGAGTTCTT 
CTGGACTACG ACAACAACAC TCTGTCCTTC TATGATGCCA TGAACTCCCA GCACATATAC 
ACTTTTGACG TTTCCTTCCT GCTTCCGGTG GCTCCCACCT TCATGATCTG GAACAAGTCA 
TTGATGATCA TGTCAGGGCT GCCGGTGCCT GACTTTGTGG ACTGCAGCGA GCAGCAAGAG 
GGCATCTGCC GCCAACAGGA GTCACCCTAT GTTTCTGGAA TAAAGAGCTG CCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mid2 ( XM_009291074.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009289349.1 Danio rerio midline 2 (mid2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa47435
Clone ID Related Accession (Same CDS sequence)	XM_009291073.2 , XM_009291074.2 , XM_009291075.2 , XM_009291074.3 , XM_009291073.3 , XM_021481165.1 , XM_009291075.3
Accession Version	XM_009291074.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2157bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	probable E3 ubiquitin-protein ligase MID2 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007125.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009291074.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAACCC TGGAGTCCGA GCTGACGTGC CCGATCTGCC TGGAACTGTT TGAGGACCCG 
CTCCTGCTCC CCTGCGCCCA CAGCCTGTGC TTCAACTGCG CCCACCGCAT CTTGGTGTCC 
CACTGCTCAA GCACCAAACC CCTCGAGTCC ATCTCTGCCT TCCAGTGCCC CACCTGCCGC 
TACGTCATCA CGCTTAACCA GCGCGGCCTG GAGGGCCTAA AGCGCAATGT GACACTGCAG 
AACATCATCG ACCGCTTCCA GAAGGCCTCT GTCAGCGGGC CTAATTCTCC AAACGAGAGT 
CGCAGGCCCC GGCCCTACAG CGCCACGCTA GGCGGGAGCG CGAGGGGCAG CCCCGCCAGC 
AGCGCCATGT CCCTCGAGCG TGTCGGGTGT CAGTTCTGTG ACCAGGAGCC TCCGCGCGAG 
GCGGTGAAGA CATGTGTGAC GTGCGAGGTG TCATACTGCG ACCGCTGCCT GAGGGCCACA 
CACCCCAATA AGAAGCCCTT CACCAGCCAT CGTCTGGTGG AGCCAGTGGC GGATGCTCAG 
CTGCGTGGAC TAGCCTGTTT GGAGCATGAA AACGAGAAGG TCAACATGTA CTGTGTGGTT 
GACGATCAGC TCATCTGCGC CCTTTGCAAG TTGGTGGGAC GCCACCGTGA ACATCAGGTC 
ACCTCGCTGA GCGATCGCTT CGACAAGCTG AAGGCTTTTC AAGTGAGCCC TGCCAGAGTT 
TCTGAAGCGG TTATGGAGGC ATCAAAGACA AGCGCCGCGC AAACCTTGGA GAATAACTTA 
ACCAATCTGG TGAAGAGGAA CAGTGAGCTG GAGAACCAGA TGGCCAAACT CATCCAGATT 
TGTCAACAGG TGGAGGTGAA CACAGCCATG CATGAAGCCA AGCTGGTGGA GGAGTGTGAT 
GAATTGGTGG AGATAATTCG CCAGAGGAAA CAGGTCATTG CTGTCAAGAT CAAAGAGTCC 
AAGGTGATGA AGCTGAGGAA GTTGGCTCAG CAGATTGCGA ACTGCAGACA GTGTCTGGAG 
CGCTCGAGCG CCCTCATCAC ACAGGCGGAG CACAGCCTGA AGGAGAACGA CCACACCCGA 
TTCCTCCAGA CGGCCAGGAA TGTGGCGGAG AGAGTTGCCA TGGCCACCGC CTCCTCCCAG 
GTCCTCATCC CAGACGTCAA TCTGAACGAG GCCTTTGACA ATTTTGCCCT TGATTTCTCC 
AGAGAAAAGA AAATCCTGGA AGGACTGGAT TATCTGACAG CTCCCAACCC TCCCTCGATA 
CGCGAAGAGC TCTGCACGGC CTCTCACGAC ACCATTACAG TGCACTGGAC ATCTGAAGAT 
GAATTCAGCG TCACGTCTTA TGAGCTTCAG TACACCATCT ACACTGGACA GACCAACTTC 
ATCAGTCTAT ATAACTCTGT GGATAGTTGG ATGATTGTAC CCAATATCAA GCAGAATCAC 
TACACAGTCC ACGGCCTGCA GAGCGGGACT CGTTACATCT TTTTCGTCAA GGCCATCAAC 
CAGGCAGGAA GCAGAAACAG TGAACCCGCT CGCCTCAAAA CCAACAGTCA GCCTTTCAAA 
CTAGACCCCA AAACGGCCCA TAAGAAACTG CGGCTCTCGA ATGACTGTCT GACAATGGAG 
AAGGATGAAA GCTCGCTGAA GAAAAGCCAC ACTCCAGAAC GCTTCAGCGG AACTGGATCT 
TATGGAGCTG CTGGCAACGT CTTCATTGAC AGTGGTTGCC ATTATTGGGA AGTACTACTG 
GGAGCCTCTA CATGGTACGC TCTTGGTGTG GCCTATAAGT CAGCACCCAA AAACGAGTGG 
AGTGGTAAAA ACTCATCATC TTGGGTTTTC TCACGCTGCA ACAATAACTT CTTGGTGCGG 
CACAACAACA AGGAGTCCAT CGTGGAAGCG TCACTACAGC TGCGCAGGGT GGGAGTTCTT 
CTGGACTACG ACAACAACAC TCTGTCCTTC TATGATGCCA TGAACTCCCA GCACATATAC 
ACTTTTGACG TTTCCTTCCT GCTTCCGGTG GCTCCCACCT TCATGATCTG GAACAAGTCA 
TTGATGATCA TGTCAGGGCT GCCGGTGCCT GACTTTGTGG ACTGCAGCGA GCAGCAAGAG 
GGCATCTGCC GCCAACAGGA GTCACCCTAT GTTTCTGGAA TAAAGAGCTG CCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009289349.1
CDS	202..2358
Translation	METLESELTCPICLELFEDPLLLPCAHSLCFNCAHRILVSHCSSTKPLESISAFQCPTCRYVITLNQRGLEGLKRNVTLQNIIDRFQKASVSGPNSPNESRRPRPYSATLGGSARGSPASSAMSLERVGCQFCDQEPPREAVKTCVTCEVSYCDRCLRATHPNKKPFTSHRLVEPVADAQLRGLACLEHENEKVNMYCVVDDQLICALCKLVGRHREHQVTSLSDRFDKLKAFQVSPARVSEAVMEASKTSAAQTLENNLTNLVKRNSELENQMAKLIQICQQVEVNTAMHEAKLVEECDELVEIIRQRKQVIAVKIKESKVMKLRKLAQQIANCRQCLERSSALITQAEHSLKENDHTRFLQTARNVAERVAMATASSQVLIPDVNLNEAFDNFALDFSREKKILEGLDYLTAPNPPSIREELCTASHDTITVHWTSEDEFSVTSYELQYTIYTGQTNFISLYNSVDSWMIVPNIKQNHYTVHGLQSGTRYIFFVKAINQAGSRNSEPARLKTNSQPFKLDPKTAHKKLRLSNDCLTMEKDESSLKKSHTPERFSGTGSYGAAGNVFIDSGCHYWEVLLGASTWYALGVAYKSAPKNEWSGKNSSSWVFSRCNNNFLVRHNNKESIVEASLQLRRVGVLLDYDNNTLSFYDAMNSQHIYTFDVSFLLPVAPTFMIWNKSLMIMSGLPVPDFVDCSEQQEGICRQQESPYVSGIKSCH

Target ORF information:

RefSeq Version	XM_009291074.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio midline 2 (mid2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009291074.2

ORF Insert Sequence:

ATGGAAACCC TGGAGTCCGA GCTGACGTGC CCGATCTGCC TGGAACTGTT TGAGGACCCG 
CTCCTGCTCC CCTGCGCCCA CAGCCTGTGC TTCAACTGCG CCCACCGCAT CTTGGTGTCC 
CACTGCTCAA GCACCAAACC CCTCGAGTCC ATCTCTGCCT TCCAGTGCCC CACCTGCCGC 
TACGTCATCA CGCTTAACCA GCGCGGCCTG GAGGGCCTAA AGCGCAATGT GACACTGCAG 
AACATCATCG ACCGCTTCCA GAAGGCCTCT GTCAGCGGGC CTAATTCTCC AAACGAGAGT 
CGCAGGCCCC GGCCCTACAG CGCCACGCTA GGCGGGAGCG CGAGGGGCAG CCCCGCCAGC 
AGCGCCATGT CCCTCGAGCG TGTCGGGTGT CAGTTCTGTG ACCAGGAGCC TCCGCGCGAG 
GCGGTGAAGA CATGTGTGAC GTGCGAGGTG TCATACTGCG ACCGCTGCCT GAGGGCCACA 
CACCCCAATA AGAAGCCCTT CACCAGCCAT CGTCTGGTGG AGCCAGTGGC GGATGCTCAG 
CTGCGTGGAC TAGCCTGTTT GGAGCATGAA AACGAGAAGG TCAACATGTA CTGTGTGGTT 
GACGATCAGC TCATCTGCGC CCTTTGCAAG TTGGTGGGAC GCCACCGTGA ACATCAGGTC 
ACCTCGCTGA GCGATCGCTT CGACAAGCTG AAGGCTTTTC AAGTGAGCCC TGCCAGAGTT 
TCTGAAGCGG TTATGGAGGC ATCAAAGACA AGCGCCGCGC AAACCTTGGA GAATAACTTA 
ACCAATCTGG TGAAGAGGAA CAGTGAGCTG GAGAACCAGA TGGCCAAACT CATCCAGATT 
TGTCAACAGG TGGAGGTGAA CACAGCCATG CATGAAGCCA AGCTGGTGGA GGAGTGTGAT 
GAATTGGTGG AGATAATTCG CCAGAGGAAA CAGGTCATTG CTGTCAAGAT CAAAGAGTCC 
AAGGTGATGA AGCTGAGGAA GTTGGCTCAG CAGATTGCGA ACTGCAGACA GTGTCTGGAG 
CGCTCGAGCG CCCTCATCAC ACAGGCGGAG CACAGCCTGA AGGAGAACGA CCACACCCGA 
TTCCTCCAGA CGGCCAGGAA TGTGGCGGAG AGAGTTGCCA TGGCCACCGC CTCCTCCCAG 
GTCCTCATCC CAGACGTCAA TCTGAACGAG GCCTTTGACA ATTTTGCCCT TGATTTCTCC 
AGAGAAAAGA AAATCCTGGA AGGACTGGAT TATCTGACAG CTCCCAACCC TCCCTCGATA 
CGCGAAGAGC TCTGCACGGC CTCTCACGAC ACCATTACAG TGCACTGGAC ATCTGAAGAT 
GAATTCAGCG TCACGTCTTA TGAGCTTCAG TACACCATCT ACACTGGACA GACCAACTTC 
ATCAGTCTAT ATAACTCTGT GGATAGTTGG ATGATTGTAC CCAATATCAA GCAGAATCAC 
TACACAGTCC ACGGCCTGCA GAGCGGGACT CGTTACATCT TTTTCGTCAA GGCCATCAAC 
CAGGCAGGAA GCAGAAACAG TGAACCCGCT CGCCTCAAAA CCAACAGTCA GCCTTTCAAA 
CTAGACCCCA AAACGGCCCA TAAGAAACTG CGGCTCTCGA ATGACTGTCT GACAATGGAG 
AAGGATGAAA GCTCGCTGAA GAAAAGCCAC ACTCCAGAAC GCTTCAGCGG AACTGGATCT 
TATGGAGCTG CTGGCAACGT CTTCATTGAC AGTGGTTGCC ATTATTGGGA AGTACTACTG 
GGAGCCTCTA CATGGTACGC TCTTGGTGTG GCCTATAAGT CAGCACCCAA AAACGAGTGG 
AGTGGTAAAA ACTCATCATC TTGGGTTTTC TCACGCTGCA ACAATAACTT CTTGGTGCGG 
CACAACAACA AGGAGTCCAT CGTGGAAGCG TCACTACAGC TGCGCAGGGT GGGAGTTCTT 
CTGGACTACG ACAACAACAC TCTGTCCTTC TATGATGCCA TGAACTCCCA GCACATATAC 
ACTTTTGACG TTTCCTTCCT GCTTCCGGTG GCTCCCACCT TCATGATCTG GAACAAGTCA 
TTGATGATCA TGTCAGGGCT GCCGGTGCCT GACTTTGTGG ACTGCAGCGA GCAGCAAGAG 
GGCATCTGCC GCCAACAGGA GTCACCCTAT GTTTCTGGAA TAAAGAGCTG CCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mid2 ( XM_009291075.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009289350.1 Danio rerio midline 2 (mid2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa47435
Clone ID Related Accession (Same CDS sequence)	XM_009291073.2 , XM_009291074.2 , XM_009291075.2 , XM_009291074.3 , XM_009291073.3 , XM_021481165.1 , XM_009291075.3
Accession Version	XM_009291075.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2157bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	probable E3 ubiquitin-protein ligase MID2 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007125.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009291075.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAACCC TGGAGTCCGA GCTGACGTGC CCGATCTGCC TGGAACTGTT TGAGGACCCG 
CTCCTGCTCC CCTGCGCCCA CAGCCTGTGC TTCAACTGCG CCCACCGCAT CTTGGTGTCC 
CACTGCTCAA GCACCAAACC CCTCGAGTCC ATCTCTGCCT TCCAGTGCCC CACCTGCCGC 
TACGTCATCA CGCTTAACCA GCGCGGCCTG GAGGGCCTAA AGCGCAATGT GACACTGCAG 
AACATCATCG ACCGCTTCCA GAAGGCCTCT GTCAGCGGGC CTAATTCTCC AAACGAGAGT 
CGCAGGCCCC GGCCCTACAG CGCCACGCTA GGCGGGAGCG CGAGGGGCAG CCCCGCCAGC 
AGCGCCATGT CCCTCGAGCG TGTCGGGTGT CAGTTCTGTG ACCAGGAGCC TCCGCGCGAG 
GCGGTGAAGA CATGTGTGAC GTGCGAGGTG TCATACTGCG ACCGCTGCCT GAGGGCCACA 
CACCCCAATA AGAAGCCCTT CACCAGCCAT CGTCTGGTGG AGCCAGTGGC GGATGCTCAG 
CTGCGTGGAC TAGCCTGTTT GGAGCATGAA AACGAGAAGG TCAACATGTA CTGTGTGGTT 
GACGATCAGC TCATCTGCGC CCTTTGCAAG TTGGTGGGAC GCCACCGTGA ACATCAGGTC 
ACCTCGCTGA GCGATCGCTT CGACAAGCTG AAGGCTTTTC AAGTGAGCCC TGCCAGAGTT 
TCTGAAGCGG TTATGGAGGC ATCAAAGACA AGCGCCGCGC AAACCTTGGA GAATAACTTA 
ACCAATCTGG TGAAGAGGAA CAGTGAGCTG GAGAACCAGA TGGCCAAACT CATCCAGATT 
TGTCAACAGG TGGAGGTGAA CACAGCCATG CATGAAGCCA AGCTGGTGGA GGAGTGTGAT 
GAATTGGTGG AGATAATTCG CCAGAGGAAA CAGGTCATTG CTGTCAAGAT CAAAGAGTCC 
AAGGTGATGA AGCTGAGGAA GTTGGCTCAG CAGATTGCGA ACTGCAGACA GTGTCTGGAG 
CGCTCGAGCG CCCTCATCAC ACAGGCGGAG CACAGCCTGA AGGAGAACGA CCACACCCGA 
TTCCTCCAGA CGGCCAGGAA TGTGGCGGAG AGAGTTGCCA TGGCCACCGC CTCCTCCCAG 
GTCCTCATCC CAGACGTCAA TCTGAACGAG GCCTTTGACA ATTTTGCCCT TGATTTCTCC 
AGAGAAAAGA AAATCCTGGA AGGACTGGAT TATCTGACAG CTCCCAACCC TCCCTCGATA 
CGCGAAGAGC TCTGCACGGC CTCTCACGAC ACCATTACAG TGCACTGGAC ATCTGAAGAT 
GAATTCAGCG TCACGTCTTA TGAGCTTCAG TACACCATCT ACACTGGACA GACCAACTTC 
ATCAGTCTAT ATAACTCTGT GGATAGTTGG ATGATTGTAC CCAATATCAA GCAGAATCAC 
TACACAGTCC ACGGCCTGCA GAGCGGGACT CGTTACATCT TTTTCGTCAA GGCCATCAAC 
CAGGCAGGAA GCAGAAACAG TGAACCCGCT CGCCTCAAAA CCAACAGTCA GCCTTTCAAA 
CTAGACCCCA AAACGGCCCA TAAGAAACTG CGGCTCTCGA ATGACTGTCT GACAATGGAG 
AAGGATGAAA GCTCGCTGAA GAAAAGCCAC ACTCCAGAAC GCTTCAGCGG AACTGGATCT 
TATGGAGCTG CTGGCAACGT CTTCATTGAC AGTGGTTGCC ATTATTGGGA AGTACTACTG 
GGAGCCTCTA CATGGTACGC TCTTGGTGTG GCCTATAAGT CAGCACCCAA AAACGAGTGG 
AGTGGTAAAA ACTCATCATC TTGGGTTTTC TCACGCTGCA ACAATAACTT CTTGGTGCGG 
CACAACAACA AGGAGTCCAT CGTGGAAGCG TCACTACAGC TGCGCAGGGT GGGAGTTCTT 
CTGGACTACG ACAACAACAC TCTGTCCTTC TATGATGCCA TGAACTCCCA GCACATATAC 
ACTTTTGACG TTTCCTTCCT GCTTCCGGTG GCTCCCACCT TCATGATCTG GAACAAGTCA 
TTGATGATCA TGTCAGGGCT GCCGGTGCCT GACTTTGTGG ACTGCAGCGA GCAGCAAGAG 
GGCATCTGCC GCCAACAGGA GTCACCCTAT GTTTCTGGAA TAAAGAGCTG CCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009289350.1
CDS	317..2473
Translation	METLESELTCPICLELFEDPLLLPCAHSLCFNCAHRILVSHCSSTKPLESISAFQCPTCRYVITLNQRGLEGLKRNVTLQNIIDRFQKASVSGPNSPNESRRPRPYSATLGGSARGSPASSAMSLERVGCQFCDQEPPREAVKTCVTCEVSYCDRCLRATHPNKKPFTSHRLVEPVADAQLRGLACLEHENEKVNMYCVVDDQLICALCKLVGRHREHQVTSLSDRFDKLKAFQVSPARVSEAVMEASKTSAAQTLENNLTNLVKRNSELENQMAKLIQICQQVEVNTAMHEAKLVEECDELVEIIRQRKQVIAVKIKESKVMKLRKLAQQIANCRQCLERSSALITQAEHSLKENDHTRFLQTARNVAERVAMATASSQVLIPDVNLNEAFDNFALDFSREKKILEGLDYLTAPNPPSIREELCTASHDTITVHWTSEDEFSVTSYELQYTIYTGQTNFISLYNSVDSWMIVPNIKQNHYTVHGLQSGTRYIFFVKAINQAGSRNSEPARLKTNSQPFKLDPKTAHKKLRLSNDCLTMEKDESSLKKSHTPERFSGTGSYGAAGNVFIDSGCHYWEVLLGASTWYALGVAYKSAPKNEWSGKNSSSWVFSRCNNNFLVRHNNKESIVEASLQLRRVGVLLDYDNNTLSFYDAMNSQHIYTFDVSFLLPVAPTFMIWNKSLMIMSGLPVPDFVDCSEQQEGICRQQESPYVSGIKSCH

Target ORF information:

RefSeq Version	XM_009291075.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio midline 2 (mid2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009291075.2

ORF Insert Sequence:

ATGGAAACCC TGGAGTCCGA GCTGACGTGC CCGATCTGCC TGGAACTGTT TGAGGACCCG 
CTCCTGCTCC CCTGCGCCCA CAGCCTGTGC TTCAACTGCG CCCACCGCAT CTTGGTGTCC 
CACTGCTCAA GCACCAAACC CCTCGAGTCC ATCTCTGCCT TCCAGTGCCC CACCTGCCGC 
TACGTCATCA CGCTTAACCA GCGCGGCCTG GAGGGCCTAA AGCGCAATGT GACACTGCAG 
AACATCATCG ACCGCTTCCA GAAGGCCTCT GTCAGCGGGC CTAATTCTCC AAACGAGAGT 
CGCAGGCCCC GGCCCTACAG CGCCACGCTA GGCGGGAGCG CGAGGGGCAG CCCCGCCAGC 
AGCGCCATGT CCCTCGAGCG TGTCGGGTGT CAGTTCTGTG ACCAGGAGCC TCCGCGCGAG 
GCGGTGAAGA CATGTGTGAC GTGCGAGGTG TCATACTGCG ACCGCTGCCT GAGGGCCACA 
CACCCCAATA AGAAGCCCTT CACCAGCCAT CGTCTGGTGG AGCCAGTGGC GGATGCTCAG 
CTGCGTGGAC TAGCCTGTTT GGAGCATGAA AACGAGAAGG TCAACATGTA CTGTGTGGTT 
GACGATCAGC TCATCTGCGC CCTTTGCAAG TTGGTGGGAC GCCACCGTGA ACATCAGGTC 
ACCTCGCTGA GCGATCGCTT CGACAAGCTG AAGGCTTTTC AAGTGAGCCC TGCCAGAGTT 
TCTGAAGCGG TTATGGAGGC ATCAAAGACA AGCGCCGCGC AAACCTTGGA GAATAACTTA 
ACCAATCTGG TGAAGAGGAA CAGTGAGCTG GAGAACCAGA TGGCCAAACT CATCCAGATT 
TGTCAACAGG TGGAGGTGAA CACAGCCATG CATGAAGCCA AGCTGGTGGA GGAGTGTGAT 
GAATTGGTGG AGATAATTCG CCAGAGGAAA CAGGTCATTG CTGTCAAGAT CAAAGAGTCC 
AAGGTGATGA AGCTGAGGAA GTTGGCTCAG CAGATTGCGA ACTGCAGACA GTGTCTGGAG 
CGCTCGAGCG CCCTCATCAC ACAGGCGGAG CACAGCCTGA AGGAGAACGA CCACACCCGA 
TTCCTCCAGA CGGCCAGGAA TGTGGCGGAG AGAGTTGCCA TGGCCACCGC CTCCTCCCAG 
GTCCTCATCC CAGACGTCAA TCTGAACGAG GCCTTTGACA ATTTTGCCCT TGATTTCTCC 
AGAGAAAAGA AAATCCTGGA AGGACTGGAT TATCTGACAG CTCCCAACCC TCCCTCGATA 
CGCGAAGAGC TCTGCACGGC CTCTCACGAC ACCATTACAG TGCACTGGAC ATCTGAAGAT 
GAATTCAGCG TCACGTCTTA TGAGCTTCAG TACACCATCT ACACTGGACA GACCAACTTC 
ATCAGTCTAT ATAACTCTGT GGATAGTTGG ATGATTGTAC CCAATATCAA GCAGAATCAC 
TACACAGTCC ACGGCCTGCA GAGCGGGACT CGTTACATCT TTTTCGTCAA GGCCATCAAC 
CAGGCAGGAA GCAGAAACAG TGAACCCGCT CGCCTCAAAA CCAACAGTCA GCCTTTCAAA 
CTAGACCCCA AAACGGCCCA TAAGAAACTG CGGCTCTCGA ATGACTGTCT GACAATGGAG 
AAGGATGAAA GCTCGCTGAA GAAAAGCCAC ACTCCAGAAC GCTTCAGCGG AACTGGATCT 
TATGGAGCTG CTGGCAACGT CTTCATTGAC AGTGGTTGCC ATTATTGGGA AGTACTACTG 
GGAGCCTCTA CATGGTACGC TCTTGGTGTG GCCTATAAGT CAGCACCCAA AAACGAGTGG 
AGTGGTAAAA ACTCATCATC TTGGGTTTTC TCACGCTGCA ACAATAACTT CTTGGTGCGG 
CACAACAACA AGGAGTCCAT CGTGGAAGCG TCACTACAGC TGCGCAGGGT GGGAGTTCTT 
CTGGACTACG ACAACAACAC TCTGTCCTTC TATGATGCCA TGAACTCCCA GCACATATAC 
ACTTTTGACG TTTCCTTCCT GCTTCCGGTG GCTCCCACCT TCATGATCTG GAACAAGTCA 
TTGATGATCA TGTCAGGGCT GCCGGTGCCT GACTTTGTGG ACTGCAGCGA GCAGCAAGAG 
GGCATCTGCC GCCAACAGGA GTCACCCTAT GTTTCTGGAA TAAAGAGCTG CCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mid2 ( XM_009291074.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009289349.1 Danio rerio midline 2 (mid2), transcript variant X2, mRNA.

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CloneID	ODa47435
Clone ID Related Accession (Same CDS sequence)	XM_009291073.2 , XM_009291074.2 , XM_009291075.2 , XM_009291074.3 , XM_009291073.3 , XM_021481165.1 , XM_009291075.3
Accession Version	XM_009291074.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2157bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	probable E3 ubiquitin-protein ligase MID2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007125.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009291074.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAACCC TGGAGTCCGA GCTGACGTGC CCGATCTGCC TGGAACTGTT TGAGGACCCG 
CTCCTGCTCC CCTGCGCCCA CAGCCTGTGC TTCAACTGCG CCCACCGCAT CTTGGTGTCC 
CACTGCTCAA GCACCAAACC CCTCGAGTCC ATCTCTGCCT TCCAGTGCCC CACCTGCCGC 
TACGTCATCA CGCTTAACCA GCGCGGCCTG GAGGGCCTAA AGCGCAATGT GACACTGCAG 
AACATCATCG ACCGCTTCCA GAAGGCCTCT GTCAGCGGGC CTAATTCTCC AAACGAGAGT 
CGCAGGCCCC GGCCCTACAG CGCCACGCTA GGCGGGAGCG CGAGGGGCAG CCCCGCCAGC 
AGCGCCATGT CCCTCGAGCG TGTCGGGTGT CAGTTCTGTG ACCAGGAGCC TCCGCGCGAG 
GCGGTGAAGA CATGTGTGAC GTGCGAGGTG TCATACTGCG ACCGCTGCCT GAGGGCCACA 
CACCCCAATA AGAAGCCCTT CACCAGCCAT CGTCTGGTGG AGCCAGTGGC GGATGCTCAG 
CTGCGTGGAC TAGCCTGTTT GGAGCATGAA AACGAGAAGG TCAACATGTA CTGTGTGGTT 
GACGATCAGC TCATCTGCGC CCTTTGCAAG TTGGTGGGAC GCCACCGTGA ACATCAGGTC 
ACCTCGCTGA GCGATCGCTT CGACAAGCTG AAGGCTTTTC AAGTGAGCCC TGCCAGAGTT 
TCTGAAGCGG TTATGGAGGC ATCAAAGACA AGCGCCGCGC AAACCTTGGA GAATAACTTA 
ACCAATCTGG TGAAGAGGAA CAGTGAGCTG GAGAACCAGA TGGCCAAACT CATCCAGATT 
TGTCAACAGG TGGAGGTGAA CACAGCCATG CATGAAGCCA AGCTGGTGGA GGAGTGTGAT 
GAATTGGTGG AGATAATTCG CCAGAGGAAA CAGGTCATTG CTGTCAAGAT CAAAGAGTCC 
AAGGTGATGA AGCTGAGGAA GTTGGCTCAG CAGATTGCGA ACTGCAGACA GTGTCTGGAG 
CGCTCGAGCG CCCTCATCAC ACAGGCGGAG CACAGCCTGA AGGAGAACGA CCACACCCGA 
TTCCTCCAGA CGGCCAGGAA TGTGGCGGAG AGAGTTGCCA TGGCCACCGC CTCCTCCCAG 
GTCCTCATCC CAGACGTCAA TCTGAACGAG GCCTTTGACA ATTTTGCCCT TGATTTCTCC 
AGAGAAAAGA AAATCCTGGA AGGACTGGAT TATCTGACAG CTCCCAACCC TCCCTCGATA 
CGCGAAGAGC TCTGCACGGC CTCTCACGAC ACCATTACAG TGCACTGGAC ATCTGAAGAT 
GAATTCAGCG TCACGTCTTA TGAGCTTCAG TACACCATCT ACACTGGACA GACCAACTTC 
ATCAGTCTAT ATAACTCTGT GGATAGTTGG ATGATTGTAC CCAATATCAA GCAGAATCAC 
TACACAGTCC ACGGCCTGCA GAGCGGGACT CGTTACATCT TTTTCGTCAA GGCCATCAAC 
CAGGCAGGAA GCAGAAACAG TGAACCCGCT CGCCTCAAAA CCAACAGTCA GCCTTTCAAA 
CTAGACCCCA AAACGGCCCA TAAGAAACTG CGGCTCTCGA ATGACTGTCT GACAATGGAG 
AAGGATGAAA GCTCGCTGAA GAAAAGCCAC ACTCCAGAAC GCTTCAGCGG AACTGGATCT 
TATGGAGCTG CTGGCAACGT CTTCATTGAC AGTGGTTGCC ATTATTGGGA AGTACTACTG 
GGAGCCTCTA CATGGTACGC TCTTGGTGTG GCCTATAAGT CAGCACCCAA AAACGAGTGG 
AGTGGTAAAA ACTCATCATC TTGGGTTTTC TCACGCTGCA ACAATAACTT CTTGGTGCGG 
CACAACAACA AGGAGTCCAT CGTGGAAGCG TCACTACAGC TGCGCAGGGT GGGAGTTCTT 
CTGGACTACG ACAACAACAC TCTGTCCTTC TATGATGCCA TGAACTCCCA GCACATATAC 
ACTTTTGACG TTTCCTTCCT GCTTCCGGTG GCTCCCACCT TCATGATCTG GAACAAGTCA 
TTGATGATCA TGTCAGGGCT GCCGGTGCCT GACTTTGTGG ACTGCAGCGA GCAGCAAGAG 
GGCATCTGCC GCCAACAGGA GTCACCCTAT GTTTCTGGAA TAAAGAGCTG CCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009289349.1
CDS	218..2374
Translation	METLESELTCPICLELFEDPLLLPCAHSLCFNCAHRILVSHCSSTKPLESISAFQCPTCRYVITLNQRGLEGLKRNVTLQNIIDRFQKASVSGPNSPNESRRPRPYSATLGGSARGSPASSAMSLERVGCQFCDQEPPREAVKTCVTCEVSYCDRCLRATHPNKKPFTSHRLVEPVADAQLRGLACLEHENEKVNMYCVVDDQLICALCKLVGRHREHQVTSLSDRFDKLKAFQVSPARVSEAVMEASKTSAAQTLENNLTNLVKRNSELENQMAKLIQICQQVEVNTAMHEAKLVEECDELVEIIRQRKQVIAVKIKESKVMKLRKLAQQIANCRQCLERSSALITQAEHSLKENDHTRFLQTARNVAERVAMATASSQVLIPDVNLNEAFDNFALDFSREKKILEGLDYLTAPNPPSIREELCTASHDTITVHWTSEDEFSVTSYELQYTIYTGQTNFISLYNSVDSWMIVPNIKQNHYTVHGLQSGTRYIFFVKAINQAGSRNSEPARLKTNSQPFKLDPKTAHKKLRLSNDCLTMEKDESSLKKSHTPERFSGTGSYGAAGNVFIDSGCHYWEVLLGASTWYALGVAYKSAPKNEWSGKNSSSWVFSRCNNNFLVRHNNKESIVEASLQLRRVGVLLDYDNNTLSFYDAMNSQHIYTFDVSFLLPVAPTFMIWNKSLMIMSGLPVPDFVDCSEQQEGICRQQESPYVSGIKSCH

Target ORF information:

RefSeq Version	XM_009291074.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio midline 2 (mid2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009291074.3

ORF Insert Sequence:

ATGGAAACCC TGGAGTCCGA GCTGACGTGC CCGATCTGCC TGGAACTGTT TGAGGACCCG 
CTCCTGCTCC CCTGCGCCCA CAGCCTGTGC TTCAACTGCG CCCACCGCAT CTTGGTGTCC 
CACTGCTCAA GCACCAAACC CCTCGAGTCC ATCTCTGCCT TCCAGTGCCC CACCTGCCGC 
TACGTCATCA CGCTTAACCA GCGCGGCCTG GAGGGCCTAA AGCGCAATGT GACACTGCAG 
AACATCATCG ACCGCTTCCA GAAGGCCTCT GTCAGCGGGC CTAATTCTCC AAACGAGAGT 
CGCAGGCCCC GGCCCTACAG CGCCACGCTA GGCGGGAGCG CGAGGGGCAG CCCCGCCAGC 
AGCGCCATGT CCCTCGAGCG TGTCGGGTGT CAGTTCTGTG ACCAGGAGCC TCCGCGCGAG 
GCGGTGAAGA CATGTGTGAC GTGCGAGGTG TCATACTGCG ACCGCTGCCT GAGGGCCACA 
CACCCCAATA AGAAGCCCTT CACCAGCCAT CGTCTGGTGG AGCCAGTGGC GGATGCTCAG 
CTGCGTGGAC TAGCCTGTTT GGAGCATGAA AACGAGAAGG TCAACATGTA CTGTGTGGTT 
GACGATCAGC TCATCTGCGC CCTTTGCAAG TTGGTGGGAC GCCACCGTGA ACATCAGGTC 
ACCTCGCTGA GCGATCGCTT CGACAAGCTG AAGGCTTTTC AAGTGAGCCC TGCCAGAGTT 
TCTGAAGCGG TTATGGAGGC ATCAAAGACA AGCGCCGCGC AAACCTTGGA GAATAACTTA 
ACCAATCTGG TGAAGAGGAA CAGTGAGCTG GAGAACCAGA TGGCCAAACT CATCCAGATT 
TGTCAACAGG TGGAGGTGAA CACAGCCATG CATGAAGCCA AGCTGGTGGA GGAGTGTGAT 
GAATTGGTGG AGATAATTCG CCAGAGGAAA CAGGTCATTG CTGTCAAGAT CAAAGAGTCC 
AAGGTGATGA AGCTGAGGAA GTTGGCTCAG CAGATTGCGA ACTGCAGACA GTGTCTGGAG 
CGCTCGAGCG CCCTCATCAC ACAGGCGGAG CACAGCCTGA AGGAGAACGA CCACACCCGA 
TTCCTCCAGA CGGCCAGGAA TGTGGCGGAG AGAGTTGCCA TGGCCACCGC CTCCTCCCAG 
GTCCTCATCC CAGACGTCAA TCTGAACGAG GCCTTTGACA ATTTTGCCCT TGATTTCTCC 
AGAGAAAAGA AAATCCTGGA AGGACTGGAT TATCTGACAG CTCCCAACCC TCCCTCGATA 
CGCGAAGAGC TCTGCACGGC CTCTCACGAC ACCATTACAG TGCACTGGAC ATCTGAAGAT 
GAATTCAGCG TCACGTCTTA TGAGCTTCAG TACACCATCT ACACTGGACA GACCAACTTC 
ATCAGTCTAT ATAACTCTGT GGATAGTTGG ATGATTGTAC CCAATATCAA GCAGAATCAC 
TACACAGTCC ACGGCCTGCA GAGCGGGACT CGTTACATCT TTTTCGTCAA GGCCATCAAC 
CAGGCAGGAA GCAGAAACAG TGAACCCGCT CGCCTCAAAA CCAACAGTCA GCCTTTCAAA 
CTAGACCCCA AAACGGCCCA TAAGAAACTG CGGCTCTCGA ATGACTGTCT GACAATGGAG 
AAGGATGAAA GCTCGCTGAA GAAAAGCCAC ACTCCAGAAC GCTTCAGCGG AACTGGATCT 
TATGGAGCTG CTGGCAACGT CTTCATTGAC AGTGGTTGCC ATTATTGGGA AGTACTACTG 
GGAGCCTCTA CATGGTACGC TCTTGGTGTG GCCTATAAGT CAGCACCCAA AAACGAGTGG 
AGTGGTAAAA ACTCATCATC TTGGGTTTTC TCACGCTGCA ACAATAACTT CTTGGTGCGG 
CACAACAACA AGGAGTCCAT CGTGGAAGCG TCACTACAGC TGCGCAGGGT GGGAGTTCTT 
CTGGACTACG ACAACAACAC TCTGTCCTTC TATGATGCCA TGAACTCCCA GCACATATAC 
ACTTTTGACG TTTCCTTCCT GCTTCCGGTG GCTCCCACCT TCATGATCTG GAACAAGTCA 
TTGATGATCA TGTCAGGGCT GCCGGTGCCT GACTTTGTGG ACTGCAGCGA GCAGCAAGAG 
GGCATCTGCC GCCAACAGGA GTCACCCTAT GTTTCTGGAA TAAAGAGCTG CCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mid2 ( XM_009291073.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009289348.1 Danio rerio midline 2 (mid2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa47435
Clone ID Related Accession (Same CDS sequence)	XM_009291073.2 , XM_009291074.2 , XM_009291075.2 , XM_009291074.3 , XM_009291073.3 , XM_021481165.1 , XM_009291075.3
Accession Version	XM_009291073.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2157bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	probable E3 ubiquitin-protein ligase MID2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007125.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009291073.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAACCC TGGAGTCCGA GCTGACGTGC CCGATCTGCC TGGAACTGTT TGAGGACCCG 
CTCCTGCTCC CCTGCGCCCA CAGCCTGTGC TTCAACTGCG CCCACCGCAT CTTGGTGTCC 
CACTGCTCAA GCACCAAACC CCTCGAGTCC ATCTCTGCCT TCCAGTGCCC CACCTGCCGC 
TACGTCATCA CGCTTAACCA GCGCGGCCTG GAGGGCCTAA AGCGCAATGT GACACTGCAG 
AACATCATCG ACCGCTTCCA GAAGGCCTCT GTCAGCGGGC CTAATTCTCC AAACGAGAGT 
CGCAGGCCCC GGCCCTACAG CGCCACGCTA GGCGGGAGCG CGAGGGGCAG CCCCGCCAGC 
AGCGCCATGT CCCTCGAGCG TGTCGGGTGT CAGTTCTGTG ACCAGGAGCC TCCGCGCGAG 
GCGGTGAAGA CATGTGTGAC GTGCGAGGTG TCATACTGCG ACCGCTGCCT GAGGGCCACA 
CACCCCAATA AGAAGCCCTT CACCAGCCAT CGTCTGGTGG AGCCAGTGGC GGATGCTCAG 
CTGCGTGGAC TAGCCTGTTT GGAGCATGAA AACGAGAAGG TCAACATGTA CTGTGTGGTT 
GACGATCAGC TCATCTGCGC CCTTTGCAAG TTGGTGGGAC GCCACCGTGA ACATCAGGTC 
ACCTCGCTGA GCGATCGCTT CGACAAGCTG AAGGCTTTTC AAGTGAGCCC TGCCAGAGTT 
TCTGAAGCGG TTATGGAGGC ATCAAAGACA AGCGCCGCGC AAACCTTGGA GAATAACTTA 
ACCAATCTGG TGAAGAGGAA CAGTGAGCTG GAGAACCAGA TGGCCAAACT CATCCAGATT 
TGTCAACAGG TGGAGGTGAA CACAGCCATG CATGAAGCCA AGCTGGTGGA GGAGTGTGAT 
GAATTGGTGG AGATAATTCG CCAGAGGAAA CAGGTCATTG CTGTCAAGAT CAAAGAGTCC 
AAGGTGATGA AGCTGAGGAA GTTGGCTCAG CAGATTGCGA ACTGCAGACA GTGTCTGGAG 
CGCTCGAGCG CCCTCATCAC ACAGGCGGAG CACAGCCTGA AGGAGAACGA CCACACCCGA 
TTCCTCCAGA CGGCCAGGAA TGTGGCGGAG AGAGTTGCCA TGGCCACCGC CTCCTCCCAG 
GTCCTCATCC CAGACGTCAA TCTGAACGAG GCCTTTGACA ATTTTGCCCT TGATTTCTCC 
AGAGAAAAGA AAATCCTGGA AGGACTGGAT TATCTGACAG CTCCCAACCC TCCCTCGATA 
CGCGAAGAGC TCTGCACGGC CTCTCACGAC ACCATTACAG TGCACTGGAC ATCTGAAGAT 
GAATTCAGCG TCACGTCTTA TGAGCTTCAG TACACCATCT ACACTGGACA GACCAACTTC 
ATCAGTCTAT ATAACTCTGT GGATAGTTGG ATGATTGTAC CCAATATCAA GCAGAATCAC 
TACACAGTCC ACGGCCTGCA GAGCGGGACT CGTTACATCT TTTTCGTCAA GGCCATCAAC 
CAGGCAGGAA GCAGAAACAG TGAACCCGCT CGCCTCAAAA CCAACAGTCA GCCTTTCAAA 
CTAGACCCCA AAACGGCCCA TAAGAAACTG CGGCTCTCGA ATGACTGTCT GACAATGGAG 
AAGGATGAAA GCTCGCTGAA GAAAAGCCAC ACTCCAGAAC GCTTCAGCGG AACTGGATCT 
TATGGAGCTG CTGGCAACGT CTTCATTGAC AGTGGTTGCC ATTATTGGGA AGTACTACTG 
GGAGCCTCTA CATGGTACGC TCTTGGTGTG GCCTATAAGT CAGCACCCAA AAACGAGTGG 
AGTGGTAAAA ACTCATCATC TTGGGTTTTC TCACGCTGCA ACAATAACTT CTTGGTGCGG 
CACAACAACA AGGAGTCCAT CGTGGAAGCG TCACTACAGC TGCGCAGGGT GGGAGTTCTT 
CTGGACTACG ACAACAACAC TCTGTCCTTC TATGATGCCA TGAACTCCCA GCACATATAC 
ACTTTTGACG TTTCCTTCCT GCTTCCGGTG GCTCCCACCT TCATGATCTG GAACAAGTCA 
TTGATGATCA TGTCAGGGCT GCCGGTGCCT GACTTTGTGG ACTGCAGCGA GCAGCAAGAG 
GGCATCTGCC GCCAACAGGA GTCACCCTAT GTTTCTGGAA TAAAGAGCTG CCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009289348.1
CDS	258..2414
Translation	METLESELTCPICLELFEDPLLLPCAHSLCFNCAHRILVSHCSSTKPLESISAFQCPTCRYVITLNQRGLEGLKRNVTLQNIIDRFQKASVSGPNSPNESRRPRPYSATLGGSARGSPASSAMSLERVGCQFCDQEPPREAVKTCVTCEVSYCDRCLRATHPNKKPFTSHRLVEPVADAQLRGLACLEHENEKVNMYCVVDDQLICALCKLVGRHREHQVTSLSDRFDKLKAFQVSPARVSEAVMEASKTSAAQTLENNLTNLVKRNSELENQMAKLIQICQQVEVNTAMHEAKLVEECDELVEIIRQRKQVIAVKIKESKVMKLRKLAQQIANCRQCLERSSALITQAEHSLKENDHTRFLQTARNVAERVAMATASSQVLIPDVNLNEAFDNFALDFSREKKILEGLDYLTAPNPPSIREELCTASHDTITVHWTSEDEFSVTSYELQYTIYTGQTNFISLYNSVDSWMIVPNIKQNHYTVHGLQSGTRYIFFVKAINQAGSRNSEPARLKTNSQPFKLDPKTAHKKLRLSNDCLTMEKDESSLKKSHTPERFSGTGSYGAAGNVFIDSGCHYWEVLLGASTWYALGVAYKSAPKNEWSGKNSSSWVFSRCNNNFLVRHNNKESIVEASLQLRRVGVLLDYDNNTLSFYDAMNSQHIYTFDVSFLLPVAPTFMIWNKSLMIMSGLPVPDFVDCSEQQEGICRQQESPYVSGIKSCH

Target ORF information:

RefSeq Version	XM_009291073.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio midline 2 (mid2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009291073.3

ORF Insert Sequence:

ATGGAAACCC TGGAGTCCGA GCTGACGTGC CCGATCTGCC TGGAACTGTT TGAGGACCCG 
CTCCTGCTCC CCTGCGCCCA CAGCCTGTGC TTCAACTGCG CCCACCGCAT CTTGGTGTCC 
CACTGCTCAA GCACCAAACC CCTCGAGTCC ATCTCTGCCT TCCAGTGCCC CACCTGCCGC 
TACGTCATCA CGCTTAACCA GCGCGGCCTG GAGGGCCTAA AGCGCAATGT GACACTGCAG 
AACATCATCG ACCGCTTCCA GAAGGCCTCT GTCAGCGGGC CTAATTCTCC AAACGAGAGT 
CGCAGGCCCC GGCCCTACAG CGCCACGCTA GGCGGGAGCG CGAGGGGCAG CCCCGCCAGC 
AGCGCCATGT CCCTCGAGCG TGTCGGGTGT CAGTTCTGTG ACCAGGAGCC TCCGCGCGAG 
GCGGTGAAGA CATGTGTGAC GTGCGAGGTG TCATACTGCG ACCGCTGCCT GAGGGCCACA 
CACCCCAATA AGAAGCCCTT CACCAGCCAT CGTCTGGTGG AGCCAGTGGC GGATGCTCAG 
CTGCGTGGAC TAGCCTGTTT GGAGCATGAA AACGAGAAGG TCAACATGTA CTGTGTGGTT 
GACGATCAGC TCATCTGCGC CCTTTGCAAG TTGGTGGGAC GCCACCGTGA ACATCAGGTC 
ACCTCGCTGA GCGATCGCTT CGACAAGCTG AAGGCTTTTC AAGTGAGCCC TGCCAGAGTT 
TCTGAAGCGG TTATGGAGGC ATCAAAGACA AGCGCCGCGC AAACCTTGGA GAATAACTTA 
ACCAATCTGG TGAAGAGGAA CAGTGAGCTG GAGAACCAGA TGGCCAAACT CATCCAGATT 
TGTCAACAGG TGGAGGTGAA CACAGCCATG CATGAAGCCA AGCTGGTGGA GGAGTGTGAT 
GAATTGGTGG AGATAATTCG CCAGAGGAAA CAGGTCATTG CTGTCAAGAT CAAAGAGTCC 
AAGGTGATGA AGCTGAGGAA GTTGGCTCAG CAGATTGCGA ACTGCAGACA GTGTCTGGAG 
CGCTCGAGCG CCCTCATCAC ACAGGCGGAG CACAGCCTGA AGGAGAACGA CCACACCCGA 
TTCCTCCAGA CGGCCAGGAA TGTGGCGGAG AGAGTTGCCA TGGCCACCGC CTCCTCCCAG 
GTCCTCATCC CAGACGTCAA TCTGAACGAG GCCTTTGACA ATTTTGCCCT TGATTTCTCC 
AGAGAAAAGA AAATCCTGGA AGGACTGGAT TATCTGACAG CTCCCAACCC TCCCTCGATA 
CGCGAAGAGC TCTGCACGGC CTCTCACGAC ACCATTACAG TGCACTGGAC ATCTGAAGAT 
GAATTCAGCG TCACGTCTTA TGAGCTTCAG TACACCATCT ACACTGGACA GACCAACTTC 
ATCAGTCTAT ATAACTCTGT GGATAGTTGG ATGATTGTAC CCAATATCAA GCAGAATCAC 
TACACAGTCC ACGGCCTGCA GAGCGGGACT CGTTACATCT TTTTCGTCAA GGCCATCAAC 
CAGGCAGGAA GCAGAAACAG TGAACCCGCT CGCCTCAAAA CCAACAGTCA GCCTTTCAAA 
CTAGACCCCA AAACGGCCCA TAAGAAACTG CGGCTCTCGA ATGACTGTCT GACAATGGAG 
AAGGATGAAA GCTCGCTGAA GAAAAGCCAC ACTCCAGAAC GCTTCAGCGG AACTGGATCT 
TATGGAGCTG CTGGCAACGT CTTCATTGAC AGTGGTTGCC ATTATTGGGA AGTACTACTG 
GGAGCCTCTA CATGGTACGC TCTTGGTGTG GCCTATAAGT CAGCACCCAA AAACGAGTGG 
AGTGGTAAAA ACTCATCATC TTGGGTTTTC TCACGCTGCA ACAATAACTT CTTGGTGCGG 
CACAACAACA AGGAGTCCAT CGTGGAAGCG TCACTACAGC TGCGCAGGGT GGGAGTTCTT 
CTGGACTACG ACAACAACAC TCTGTCCTTC TATGATGCCA TGAACTCCCA GCACATATAC 
ACTTTTGACG TTTCCTTCCT GCTTCCGGTG GCTCCCACCT TCATGATCTG GAACAAGTCA 
TTGATGATCA TGTCAGGGCT GCCGGTGCCT GACTTTGTGG ACTGCAGCGA GCAGCAAGAG 
GGCATCTGCC GCCAACAGGA GTCACCCTAT GTTTCTGGAA TAAAGAGCTG CCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mid2 ( XM_021481165.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021336840.1 Danio rerio midline 2 (mid2), transcript variant X3, mRNA.

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CloneID	ODa47435
Clone ID Related Accession (Same CDS sequence)	XM_009291073.2 , XM_009291074.2 , XM_009291075.2 , XM_009291074.3 , XM_009291073.3 , XM_021481165.1 , XM_009291075.3
Accession Version	XM_021481165.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2157bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	probable E3 ubiquitin-protein ligase MID2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007125.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAACCC TGGAGTCCGA GCTGACGTGC CCGATCTGCC TGGAACTGTT TGAGGACCCG 
CTCCTGCTCC CCTGCGCCCA CAGCCTGTGC TTCAACTGCG CCCACCGCAT CTTGGTGTCC 
CACTGCTCAA GCACCAAACC CCTCGAGTCC ATCTCTGCCT TCCAGTGCCC CACCTGCCGC 
TACGTCATCA CGCTTAACCA GCGCGGCCTG GAGGGCCTAA AGCGCAATGT GACACTGCAG 
AACATCATCG ACCGCTTCCA GAAGGCCTCT GTCAGCGGGC CTAATTCTCC AAACGAGAGT 
CGCAGGCCCC GGCCCTACAG CGCCACGCTA GGCGGGAGCG CGAGGGGCAG CCCCGCCAGC 
AGCGCCATGT CCCTCGAGCG TGTCGGGTGT CAGTTCTGTG ACCAGGAGCC TCCGCGCGAG 
GCGGTGAAGA CATGTGTGAC GTGCGAGGTG TCATACTGCG ACCGCTGCCT GAGGGCCACA 
CACCCCAATA AGAAGCCCTT CACCAGCCAT CGTCTGGTGG AGCCAGTGGC GGATGCTCAG 
CTGCGTGGAC TAGCCTGTTT GGAGCATGAA AACGAGAAGG TCAACATGTA CTGTGTGGTT 
GACGATCAGC TCATCTGCGC CCTTTGCAAG TTGGTGGGAC GCCACCGTGA ACATCAGGTC 
ACCTCGCTGA GCGATCGCTT CGACAAGCTG AAGGCTTTTC AAGTGAGCCC TGCCAGAGTT 
TCTGAAGCGG TTATGGAGGC ATCAAAGACA AGCGCCGCGC AAACCTTGGA GAATAACTTA 
ACCAATCTGG TGAAGAGGAA CAGTGAGCTG GAGAACCAGA TGGCCAAACT CATCCAGATT 
TGTCAACAGG TGGAGGTGAA CACAGCCATG CATGAAGCCA AGCTGGTGGA GGAGTGTGAT 
GAATTGGTGG AGATAATTCG CCAGAGGAAA CAGGTCATTG CTGTCAAGAT CAAAGAGTCC 
AAGGTGATGA AGCTGAGGAA GTTGGCTCAG CAGATTGCGA ACTGCAGACA GTGTCTGGAG 
CGCTCGAGCG CCCTCATCAC ACAGGCGGAG CACAGCCTGA AGGAGAACGA CCACACCCGA 
TTCCTCCAGA CGGCCAGGAA TGTGGCGGAG AGAGTTGCCA TGGCCACCGC CTCCTCCCAG 
GTCCTCATCC CAGACGTCAA TCTGAACGAG GCCTTTGACA ATTTTGCCCT TGATTTCTCC 
AGAGAAAAGA AAATCCTGGA AGGACTGGAT TATCTGACAG CTCCCAACCC TCCCTCGATA 
CGCGAAGAGC TCTGCACGGC CTCTCACGAC ACCATTACAG TGCACTGGAC ATCTGAAGAT 
GAATTCAGCG TCACGTCTTA TGAGCTTCAG TACACCATCT ACACTGGACA GACCAACTTC 
ATCAGTCTAT ATAACTCTGT GGATAGTTGG ATGATTGTAC CCAATATCAA GCAGAATCAC 
TACACAGTCC ACGGCCTGCA GAGCGGGACT CGTTACATCT TTTTCGTCAA GGCCATCAAC 
CAGGCAGGAA GCAGAAACAG TGAACCCGCT CGCCTCAAAA CCAACAGTCA GCCTTTCAAA 
CTAGACCCCA AAACGGCCCA TAAGAAACTG CGGCTCTCGA ATGACTGTCT GACAATGGAG 
AAGGATGAAA GCTCGCTGAA GAAAAGCCAC ACTCCAGAAC GCTTCAGCGG AACTGGATCT 
TATGGAGCTG CTGGCAACGT CTTCATTGAC AGTGGTTGCC ATTATTGGGA AGTACTACTG 
GGAGCCTCTA CATGGTACGC TCTTGGTGTG GCCTATAAGT CAGCACCCAA AAACGAGTGG 
AGTGGTAAAA ACTCATCATC TTGGGTTTTC TCACGCTGCA ACAATAACTT CTTGGTGCGG 
CACAACAACA AGGAGTCCAT CGTGGAAGCG TCACTACAGC TGCGCAGGGT GGGAGTTCTT 
CTGGACTACG ACAACAACAC TCTGTCCTTC TATGATGCCA TGAACTCCCA GCACATATAC 
ACTTTTGACG TTTCCTTCCT GCTTCCGGTG GCTCCCACCT TCATGATCTG GAACAAGTCA 
TTGATGATCA TGTCAGGGCT GCCGGTGCCT GACTTTGTGG ACTGCAGCGA GCAGCAAGAG 
GGCATCTGCC GCCAACAGGA GTCACCCTAT GTTTCTGGAA TAAAGAGCTG CCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021336840.1
CDS	427..2583
Translation	METLESELTCPICLELFEDPLLLPCAHSLCFNCAHRILVSHCSSTKPLESISAFQCPTCRYVITLNQRGLEGLKRNVTLQNIIDRFQKASVSGPNSPNESRRPRPYSATLGGSARGSPASSAMSLERVGCQFCDQEPPREAVKTCVTCEVSYCDRCLRATHPNKKPFTSHRLVEPVADAQLRGLACLEHENEKVNMYCVVDDQLICALCKLVGRHREHQVTSLSDRFDKLKAFQVSPARVSEAVMEASKTSAAQTLENNLTNLVKRNSELENQMAKLIQICQQVEVNTAMHEAKLVEECDELVEIIRQRKQVIAVKIKESKVMKLRKLAQQIANCRQCLERSSALITQAEHSLKENDHTRFLQTARNVAERVAMATASSQVLIPDVNLNEAFDNFALDFSREKKILEGLDYLTAPNPPSIREELCTASHDTITVHWTSEDEFSVTSYELQYTIYTGQTNFISLYNSVDSWMIVPNIKQNHYTVHGLQSGTRYIFFVKAINQAGSRNSEPARLKTNSQPFKLDPKTAHKKLRLSNDCLTMEKDESSLKKSHTPERFSGTGSYGAAGNVFIDSGCHYWEVLLGASTWYALGVAYKSAPKNEWSGKNSSSWVFSRCNNNFLVRHNNKESIVEASLQLRRVGVLLDYDNNTLSFYDAMNSQHIYTFDVSFLLPVAPTFMIWNKSLMIMSGLPVPDFVDCSEQQEGICRQQESPYVSGIKSCH

Target ORF information:

RefSeq Version	XM_021481165.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio midline 2 (mid2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021481165.1

ORF Insert Sequence:

ATGGAAACCC TGGAGTCCGA GCTGACGTGC CCGATCTGCC TGGAACTGTT TGAGGACCCG 
CTCCTGCTCC CCTGCGCCCA CAGCCTGTGC TTCAACTGCG CCCACCGCAT CTTGGTGTCC 
CACTGCTCAA GCACCAAACC CCTCGAGTCC ATCTCTGCCT TCCAGTGCCC CACCTGCCGC 
TACGTCATCA CGCTTAACCA GCGCGGCCTG GAGGGCCTAA AGCGCAATGT GACACTGCAG 
AACATCATCG ACCGCTTCCA GAAGGCCTCT GTCAGCGGGC CTAATTCTCC AAACGAGAGT 
CGCAGGCCCC GGCCCTACAG CGCCACGCTA GGCGGGAGCG CGAGGGGCAG CCCCGCCAGC 
AGCGCCATGT CCCTCGAGCG TGTCGGGTGT CAGTTCTGTG ACCAGGAGCC TCCGCGCGAG 
GCGGTGAAGA CATGTGTGAC GTGCGAGGTG TCATACTGCG ACCGCTGCCT GAGGGCCACA 
CACCCCAATA AGAAGCCCTT CACCAGCCAT CGTCTGGTGG AGCCAGTGGC GGATGCTCAG 
CTGCGTGGAC TAGCCTGTTT GGAGCATGAA AACGAGAAGG TCAACATGTA CTGTGTGGTT 
GACGATCAGC TCATCTGCGC CCTTTGCAAG TTGGTGGGAC GCCACCGTGA ACATCAGGTC 
ACCTCGCTGA GCGATCGCTT CGACAAGCTG AAGGCTTTTC AAGTGAGCCC TGCCAGAGTT 
TCTGAAGCGG TTATGGAGGC ATCAAAGACA AGCGCCGCGC AAACCTTGGA GAATAACTTA 
ACCAATCTGG TGAAGAGGAA CAGTGAGCTG GAGAACCAGA TGGCCAAACT CATCCAGATT 
TGTCAACAGG TGGAGGTGAA CACAGCCATG CATGAAGCCA AGCTGGTGGA GGAGTGTGAT 
GAATTGGTGG AGATAATTCG CCAGAGGAAA CAGGTCATTG CTGTCAAGAT CAAAGAGTCC 
AAGGTGATGA AGCTGAGGAA GTTGGCTCAG CAGATTGCGA ACTGCAGACA GTGTCTGGAG 
CGCTCGAGCG CCCTCATCAC ACAGGCGGAG CACAGCCTGA AGGAGAACGA CCACACCCGA 
TTCCTCCAGA CGGCCAGGAA TGTGGCGGAG AGAGTTGCCA TGGCCACCGC CTCCTCCCAG 
GTCCTCATCC CAGACGTCAA TCTGAACGAG GCCTTTGACA ATTTTGCCCT TGATTTCTCC 
AGAGAAAAGA AAATCCTGGA AGGACTGGAT TATCTGACAG CTCCCAACCC TCCCTCGATA 
CGCGAAGAGC TCTGCACGGC CTCTCACGAC ACCATTACAG TGCACTGGAC ATCTGAAGAT 
GAATTCAGCG TCACGTCTTA TGAGCTTCAG TACACCATCT ACACTGGACA GACCAACTTC 
ATCAGTCTAT ATAACTCTGT GGATAGTTGG ATGATTGTAC CCAATATCAA GCAGAATCAC 
TACACAGTCC ACGGCCTGCA GAGCGGGACT CGTTACATCT TTTTCGTCAA GGCCATCAAC 
CAGGCAGGAA GCAGAAACAG TGAACCCGCT CGCCTCAAAA CCAACAGTCA GCCTTTCAAA 
CTAGACCCCA AAACGGCCCA TAAGAAACTG CGGCTCTCGA ATGACTGTCT GACAATGGAG 
AAGGATGAAA GCTCGCTGAA GAAAAGCCAC ACTCCAGAAC GCTTCAGCGG AACTGGATCT 
TATGGAGCTG CTGGCAACGT CTTCATTGAC AGTGGTTGCC ATTATTGGGA AGTACTACTG 
GGAGCCTCTA CATGGTACGC TCTTGGTGTG GCCTATAAGT CAGCACCCAA AAACGAGTGG 
AGTGGTAAAA ACTCATCATC TTGGGTTTTC TCACGCTGCA ACAATAACTT CTTGGTGCGG 
CACAACAACA AGGAGTCCAT CGTGGAAGCG TCACTACAGC TGCGCAGGGT GGGAGTTCTT 
CTGGACTACG ACAACAACAC TCTGTCCTTC TATGATGCCA TGAACTCCCA GCACATATAC 
ACTTTTGACG TTTCCTTCCT GCTTCCGGTG GCTCCCACCT TCATGATCTG GAACAAGTCA 
TTGATGATCA TGTCAGGGCT GCCGGTGCCT GACTTTGTGG ACTGCAGCGA GCAGCAAGAG 
GGCATCTGCC GCCAACAGGA GTCACCCTAT GTTTCTGGAA TAAAGAGCTG CCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mid2 ( XM_009291075.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009289350.1 Danio rerio midline 2 (mid2), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ODa47435
Clone ID Related Accession (Same CDS sequence)	XM_009291073.2 , XM_009291074.2 , XM_009291075.2 , XM_009291074.3 , XM_009291073.3 , XM_021481165.1 , XM_009291075.3
Accession Version	XM_009291075.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2157bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	probable E3 ubiquitin-protein ligase MID2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007125.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009291075.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAACCC TGGAGTCCGA GCTGACGTGC CCGATCTGCC TGGAACTGTT TGAGGACCCG 
CTCCTGCTCC CCTGCGCCCA CAGCCTGTGC TTCAACTGCG CCCACCGCAT CTTGGTGTCC 
CACTGCTCAA GCACCAAACC CCTCGAGTCC ATCTCTGCCT TCCAGTGCCC CACCTGCCGC 
TACGTCATCA CGCTTAACCA GCGCGGCCTG GAGGGCCTAA AGCGCAATGT GACACTGCAG 
AACATCATCG ACCGCTTCCA GAAGGCCTCT GTCAGCGGGC CTAATTCTCC AAACGAGAGT 
CGCAGGCCCC GGCCCTACAG CGCCACGCTA GGCGGGAGCG CGAGGGGCAG CCCCGCCAGC 
AGCGCCATGT CCCTCGAGCG TGTCGGGTGT CAGTTCTGTG ACCAGGAGCC TCCGCGCGAG 
GCGGTGAAGA CATGTGTGAC GTGCGAGGTG TCATACTGCG ACCGCTGCCT GAGGGCCACA 
CACCCCAATA AGAAGCCCTT CACCAGCCAT CGTCTGGTGG AGCCAGTGGC GGATGCTCAG 
CTGCGTGGAC TAGCCTGTTT GGAGCATGAA AACGAGAAGG TCAACATGTA CTGTGTGGTT 
GACGATCAGC TCATCTGCGC CCTTTGCAAG TTGGTGGGAC GCCACCGTGA ACATCAGGTC 
ACCTCGCTGA GCGATCGCTT CGACAAGCTG AAGGCTTTTC AAGTGAGCCC TGCCAGAGTT 
TCTGAAGCGG TTATGGAGGC ATCAAAGACA AGCGCCGCGC AAACCTTGGA GAATAACTTA 
ACCAATCTGG TGAAGAGGAA CAGTGAGCTG GAGAACCAGA TGGCCAAACT CATCCAGATT 
TGTCAACAGG TGGAGGTGAA CACAGCCATG CATGAAGCCA AGCTGGTGGA GGAGTGTGAT 
GAATTGGTGG AGATAATTCG CCAGAGGAAA CAGGTCATTG CTGTCAAGAT CAAAGAGTCC 
AAGGTGATGA AGCTGAGGAA GTTGGCTCAG CAGATTGCGA ACTGCAGACA GTGTCTGGAG 
CGCTCGAGCG CCCTCATCAC ACAGGCGGAG CACAGCCTGA AGGAGAACGA CCACACCCGA 
TTCCTCCAGA CGGCCAGGAA TGTGGCGGAG AGAGTTGCCA TGGCCACCGC CTCCTCCCAG 
GTCCTCATCC CAGACGTCAA TCTGAACGAG GCCTTTGACA ATTTTGCCCT TGATTTCTCC 
AGAGAAAAGA AAATCCTGGA AGGACTGGAT TATCTGACAG CTCCCAACCC TCCCTCGATA 
CGCGAAGAGC TCTGCACGGC CTCTCACGAC ACCATTACAG TGCACTGGAC ATCTGAAGAT 
GAATTCAGCG TCACGTCTTA TGAGCTTCAG TACACCATCT ACACTGGACA GACCAACTTC 
ATCAGTCTAT ATAACTCTGT GGATAGTTGG ATGATTGTAC CCAATATCAA GCAGAATCAC 
TACACAGTCC ACGGCCTGCA GAGCGGGACT CGTTACATCT TTTTCGTCAA GGCCATCAAC 
CAGGCAGGAA GCAGAAACAG TGAACCCGCT CGCCTCAAAA CCAACAGTCA GCCTTTCAAA 
CTAGACCCCA AAACGGCCCA TAAGAAACTG CGGCTCTCGA ATGACTGTCT GACAATGGAG 
AAGGATGAAA GCTCGCTGAA GAAAAGCCAC ACTCCAGAAC GCTTCAGCGG AACTGGATCT 
TATGGAGCTG CTGGCAACGT CTTCATTGAC AGTGGTTGCC ATTATTGGGA AGTACTACTG 
GGAGCCTCTA CATGGTACGC TCTTGGTGTG GCCTATAAGT CAGCACCCAA AAACGAGTGG 
AGTGGTAAAA ACTCATCATC TTGGGTTTTC TCACGCTGCA ACAATAACTT CTTGGTGCGG 
CACAACAACA AGGAGTCCAT CGTGGAAGCG TCACTACAGC TGCGCAGGGT GGGAGTTCTT 
CTGGACTACG ACAACAACAC TCTGTCCTTC TATGATGCCA TGAACTCCCA GCACATATAC 
ACTTTTGACG TTTCCTTCCT GCTTCCGGTG GCTCCCACCT TCATGATCTG GAACAAGTCA 
TTGATGATCA TGTCAGGGCT GCCGGTGCCT GACTTTGTGG ACTGCAGCGA GCAGCAAGAG 
GGCATCTGCC GCCAACAGGA GTCACCCTAT GTTTCTGGAA TAAAGAGCTG CCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009289350.1
CDS	388..2544
Translation	METLESELTCPICLELFEDPLLLPCAHSLCFNCAHRILVSHCSSTKPLESISAFQCPTCRYVITLNQRGLEGLKRNVTLQNIIDRFQKASVSGPNSPNESRRPRPYSATLGGSARGSPASSAMSLERVGCQFCDQEPPREAVKTCVTCEVSYCDRCLRATHPNKKPFTSHRLVEPVADAQLRGLACLEHENEKVNMYCVVDDQLICALCKLVGRHREHQVTSLSDRFDKLKAFQVSPARVSEAVMEASKTSAAQTLENNLTNLVKRNSELENQMAKLIQICQQVEVNTAMHEAKLVEECDELVEIIRQRKQVIAVKIKESKVMKLRKLAQQIANCRQCLERSSALITQAEHSLKENDHTRFLQTARNVAERVAMATASSQVLIPDVNLNEAFDNFALDFSREKKILEGLDYLTAPNPPSIREELCTASHDTITVHWTSEDEFSVTSYELQYTIYTGQTNFISLYNSVDSWMIVPNIKQNHYTVHGLQSGTRYIFFVKAINQAGSRNSEPARLKTNSQPFKLDPKTAHKKLRLSNDCLTMEKDESSLKKSHTPERFSGTGSYGAAGNVFIDSGCHYWEVLLGASTWYALGVAYKSAPKNEWSGKNSSSWVFSRCNNNFLVRHNNKESIVEASLQLRRVGVLLDYDNNTLSFYDAMNSQHIYTFDVSFLLPVAPTFMIWNKSLMIMSGLPVPDFVDCSEQQEGICRQQESPYVSGIKSCH

Target ORF information:

RefSeq Version	XM_009291075.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio midline 2 (mid2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009291075.3

ORF Insert Sequence:

ATGGAAACCC TGGAGTCCGA GCTGACGTGC CCGATCTGCC TGGAACTGTT TGAGGACCCG 
CTCCTGCTCC CCTGCGCCCA CAGCCTGTGC TTCAACTGCG CCCACCGCAT CTTGGTGTCC 
CACTGCTCAA GCACCAAACC CCTCGAGTCC ATCTCTGCCT TCCAGTGCCC CACCTGCCGC 
TACGTCATCA CGCTTAACCA GCGCGGCCTG GAGGGCCTAA AGCGCAATGT GACACTGCAG 
AACATCATCG ACCGCTTCCA GAAGGCCTCT GTCAGCGGGC CTAATTCTCC AAACGAGAGT 
CGCAGGCCCC GGCCCTACAG CGCCACGCTA GGCGGGAGCG CGAGGGGCAG CCCCGCCAGC 
AGCGCCATGT CCCTCGAGCG TGTCGGGTGT CAGTTCTGTG ACCAGGAGCC TCCGCGCGAG 
GCGGTGAAGA CATGTGTGAC GTGCGAGGTG TCATACTGCG ACCGCTGCCT GAGGGCCACA 
CACCCCAATA AGAAGCCCTT CACCAGCCAT CGTCTGGTGG AGCCAGTGGC GGATGCTCAG 
CTGCGTGGAC TAGCCTGTTT GGAGCATGAA AACGAGAAGG TCAACATGTA CTGTGTGGTT 
GACGATCAGC TCATCTGCGC CCTTTGCAAG TTGGTGGGAC GCCACCGTGA ACATCAGGTC 
ACCTCGCTGA GCGATCGCTT CGACAAGCTG AAGGCTTTTC AAGTGAGCCC TGCCAGAGTT 
TCTGAAGCGG TTATGGAGGC ATCAAAGACA AGCGCCGCGC AAACCTTGGA GAATAACTTA 
ACCAATCTGG TGAAGAGGAA CAGTGAGCTG GAGAACCAGA TGGCCAAACT CATCCAGATT 
TGTCAACAGG TGGAGGTGAA CACAGCCATG CATGAAGCCA AGCTGGTGGA GGAGTGTGAT 
GAATTGGTGG AGATAATTCG CCAGAGGAAA CAGGTCATTG CTGTCAAGAT CAAAGAGTCC 
AAGGTGATGA AGCTGAGGAA GTTGGCTCAG CAGATTGCGA ACTGCAGACA GTGTCTGGAG 
CGCTCGAGCG CCCTCATCAC ACAGGCGGAG CACAGCCTGA AGGAGAACGA CCACACCCGA 
TTCCTCCAGA CGGCCAGGAA TGTGGCGGAG AGAGTTGCCA TGGCCACCGC CTCCTCCCAG 
GTCCTCATCC CAGACGTCAA TCTGAACGAG GCCTTTGACA ATTTTGCCCT TGATTTCTCC 
AGAGAAAAGA AAATCCTGGA AGGACTGGAT TATCTGACAG CTCCCAACCC TCCCTCGATA 
CGCGAAGAGC TCTGCACGGC CTCTCACGAC ACCATTACAG TGCACTGGAC ATCTGAAGAT 
GAATTCAGCG TCACGTCTTA TGAGCTTCAG TACACCATCT ACACTGGACA GACCAACTTC 
ATCAGTCTAT ATAACTCTGT GGATAGTTGG ATGATTGTAC CCAATATCAA GCAGAATCAC 
TACACAGTCC ACGGCCTGCA GAGCGGGACT CGTTACATCT TTTTCGTCAA GGCCATCAAC 
CAGGCAGGAA GCAGAAACAG TGAACCCGCT CGCCTCAAAA CCAACAGTCA GCCTTTCAAA 
CTAGACCCCA AAACGGCCCA TAAGAAACTG CGGCTCTCGA ATGACTGTCT GACAATGGAG 
AAGGATGAAA GCTCGCTGAA GAAAAGCCAC ACTCCAGAAC GCTTCAGCGG AACTGGATCT 
TATGGAGCTG CTGGCAACGT CTTCATTGAC AGTGGTTGCC ATTATTGGGA AGTACTACTG 
GGAGCCTCTA CATGGTACGC TCTTGGTGTG GCCTATAAGT CAGCACCCAA AAACGAGTGG 
AGTGGTAAAA ACTCATCATC TTGGGTTTTC TCACGCTGCA ACAATAACTT CTTGGTGCGG 
CACAACAACA AGGAGTCCAT CGTGGAAGCG TCACTACAGC TGCGCAGGGT GGGAGTTCTT 
CTGGACTACG ACAACAACAC TCTGTCCTTC TATGATGCCA TGAACTCCCA GCACATATAC 
ACTTTTGACG TTTCCTTCCT GCTTCCGGTG GCTCCCACCT TCATGATCTG GAACAAGTCA 
TTGATGATCA TGTCAGGGCT GCCGGTGCCT GACTTTGTGG ACTGCAGCGA GCAGCAAGAG 
GGCATCTGCC GCCAACAGGA GTCACCCTAT GTTTCTGGAA TAAAGAGCTG CCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mid2 ( XM_009291076.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009289351.1 Danio rerio midline 2 (mid2), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ODa47436
Clone ID Related Accession (Same CDS sequence)	XM_009291076.2 , XM_009291077.2 , XM_009291077.3 , XM_009291076.3
Accession Version	XM_009291076.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2091bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	probable E3 ubiquitin-protein ligase MID2 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007125.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009291076.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAACCC TGGAGTCCGA GCTGACGTGC CCGATCTGCC TGGAACTGTT TGAGGACCCG 
CTCCTGCTCC CCTGCGCCCA CAGCCTGTGC TTCAACTGCG CCCACCGCAT CTTGGTGTCC 
CACTGCTCAA GCACCAAACC CCTCGAGTCC ATCTCTGCCT TCCAGTGCCC CACCTGCCGC 
TACGTCATCA CGCTTAACCA GCGCGGCCTG GAGGGCCTAA AGCGCAATGT GACACTGCAG 
AACATCATCG ACCGCTTCCA GAAGGCCTCT GTCAGCGGGC CTAATTCTCC AAACGAGAGT 
CGCAGGCCCC GGCCCTACAG CGCCACGCTA GGCGGGAGCG CGAGGGGCAG CCCCGCCAGC 
AGCGCCATGT CCCTCGAGCG TGTCGGGTGT CAGTTCTGTG ACCAGGAGCC TCCGCGCGAG 
GCGGTGAAGA CATGTGTGAC GTGCGAGGTG TCATACTGCG ACCGCTGCCT GAGGGCCACA 
CACCCCAATA AGAAGCCCTT CACCAGCCAT CGTCTGGTGG AGCCAGTGGC GGATGCTCAG 
CTGCGTGGAC TAGCCTGTTT GGAGCATGAA AACGAGAAGG TCAACATGTA CTGTGTGGTT 
GACGATCAGC TCATCTGCGC CCTTTGCAAG TTGGTGGGAC GCCACCGTGA ACATCAGGTC 
ACCTCGCTGA GCGATCGCTT CGACAAGCTG AAGCAAACCT TGGAGAATAA CTTAACCAAT 
CTGGTGAAGA GGAACAGTGA GCTGGAGAAC CAGATGGCCA AACTCATCCA GATTTGTCAA 
CAGGTGGAGG TGAACACAGC CATGCATGAA GCCAAGCTGG TGGAGGAGTG TGATGAATTG 
GTGGAGATAA TTCGCCAGAG GAAACAGGTC ATTGCTGTCA AGATCAAAGA GTCCAAGGTG 
ATGAAGCTGA GGAAGTTGGC TCAGCAGATT GCGAACTGCA GACAGTGTCT GGAGCGCTCG 
AGCGCCCTCA TCACACAGGC GGAGCACAGC CTGAAGGAGA ACGACCACAC CCGATTCCTC 
CAGACGGCCA GGAATGTGGC GGAGAGAGTT GCCATGGCCA CCGCCTCCTC CCAGGTCCTC 
ATCCCAGACG TCAATCTGAA CGAGGCCTTT GACAATTTTG CCCTTGATTT CTCCAGAGAA 
AAGAAAATCC TGGAAGGACT GGATTATCTG ACAGCTCCCA ACCCTCCCTC GATACGCGAA 
GAGCTCTGCA CGGCCTCTCA CGACACCATT ACAGTGCACT GGACATCTGA AGATGAATTC 
AGCGTCACGT CTTATGAGCT TCAGTACACC ATCTACACTG GACAGACCAA CTTCATCAGT 
CTATATAACT CTGTGGATAG TTGGATGATT GTACCCAATA TCAAGCAGAA TCACTACACA 
GTCCACGGCC TGCAGAGCGG GACTCGTTAC ATCTTTTTCG TCAAGGCCAT CAACCAGGCA 
GGAAGCAGAA ACAGTGAACC CGCTCGCCTC AAAACCAACA GTCAGCCTTT CAAACTAGAC 
CCCAAAACGG CCCATAAGAA ACTGCGGCTC TCGAATGACT GTCTGACAAT GGAGAAGGAT 
GAAAGCTCGC TGAAGAAAAG CCACACTCCA GAACGCTTCA GCGGAACTGG ATCTTATGGA 
GCTGCTGGCA ACGTCTTCAT TGACAGTGGT TGCCATTATT GGGAAGTACT ACTGGGAGCC 
TCTACATGGT ACGCTCTTGG TGTGGCCTAT AAGTCAGCAC CCAAAAACGA GTGGAGTGGT 
AAAAACTCAT CATCTTGGGT TTTCTCACGC TGCAACAATA ACTTCTTGGT GCGGCACAAC 
AACAAGGAGT CCATCGTGGA AGCGTCACTA CAGCTGCGCA GGGTGGGAGT TCTTCTGGAC 
TACGACAACA ACACTCTGTC CTTCTATGAT GCCATGAACT CCCAGCACAT ATACACTTTT 
GACGTTTCCT TCCTGCTTCC GGTGGCTCCC ACCTTCATGA TCTGGAACAA GTCATTGATG 
ATCATGTCAG GGCTGCCGGT GCCTGACTTT GTGGACTGCA GCGAGCAGCA AGAGGGCATC 
TGCCGCCAAC AGGAGTCACC CTATGTTTCT GGAATAAAGA GCTGCCACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009289351.1
CDS	242..2332
Translation	METLESELTCPICLELFEDPLLLPCAHSLCFNCAHRILVSHCSSTKPLESISAFQCPTCRYVITLNQRGLEGLKRNVTLQNIIDRFQKASVSGPNSPNESRRPRPYSATLGGSARGSPASSAMSLERVGCQFCDQEPPREAVKTCVTCEVSYCDRCLRATHPNKKPFTSHRLVEPVADAQLRGLACLEHENEKVNMYCVVDDQLICALCKLVGRHREHQVTSLSDRFDKLKQTLENNLTNLVKRNSELENQMAKLIQICQQVEVNTAMHEAKLVEECDELVEIIRQRKQVIAVKIKESKVMKLRKLAQQIANCRQCLERSSALITQAEHSLKENDHTRFLQTARNVAERVAMATASSQVLIPDVNLNEAFDNFALDFSREKKILEGLDYLTAPNPPSIREELCTASHDTITVHWTSEDEFSVTSYELQYTIYTGQTNFISLYNSVDSWMIVPNIKQNHYTVHGLQSGTRYIFFVKAINQAGSRNSEPARLKTNSQPFKLDPKTAHKKLRLSNDCLTMEKDESSLKKSHTPERFSGTGSYGAAGNVFIDSGCHYWEVLLGASTWYALGVAYKSAPKNEWSGKNSSSWVFSRCNNNFLVRHNNKESIVEASLQLRRVGVLLDYDNNTLSFYDAMNSQHIYTFDVSFLLPVAPTFMIWNKSLMIMSGLPVPDFVDCSEQQEGICRQQESPYVSGIKSCH

Target ORF information:

RefSeq Version	XM_009291076.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio midline 2 (mid2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009291076.2

ORF Insert Sequence:

ATGGAAACCC TGGAGTCCGA GCTGACGTGC CCGATCTGCC TGGAACTGTT TGAGGACCCG 
CTCCTGCTCC CCTGCGCCCA CAGCCTGTGC TTCAACTGCG CCCACCGCAT CTTGGTGTCC 
CACTGCTCAA GCACCAAACC CCTCGAGTCC ATCTCTGCCT TCCAGTGCCC CACCTGCCGC 
TACGTCATCA CGCTTAACCA GCGCGGCCTG GAGGGCCTAA AGCGCAATGT GACACTGCAG 
AACATCATCG ACCGCTTCCA GAAGGCCTCT GTCAGCGGGC CTAATTCTCC AAACGAGAGT 
CGCAGGCCCC GGCCCTACAG CGCCACGCTA GGCGGGAGCG CGAGGGGCAG CCCCGCCAGC 
AGCGCCATGT CCCTCGAGCG TGTCGGGTGT CAGTTCTGTG ACCAGGAGCC TCCGCGCGAG 
GCGGTGAAGA CATGTGTGAC GTGCGAGGTG TCATACTGCG ACCGCTGCCT GAGGGCCACA 
CACCCCAATA AGAAGCCCTT CACCAGCCAT CGTCTGGTGG AGCCAGTGGC GGATGCTCAG 
CTGCGTGGAC TAGCCTGTTT GGAGCATGAA AACGAGAAGG TCAACATGTA CTGTGTGGTT 
GACGATCAGC TCATCTGCGC CCTTTGCAAG TTGGTGGGAC GCCACCGTGA ACATCAGGTC 
ACCTCGCTGA GCGATCGCTT CGACAAGCTG AAGCAAACCT TGGAGAATAA CTTAACCAAT 
CTGGTGAAGA GGAACAGTGA GCTGGAGAAC CAGATGGCCA AACTCATCCA GATTTGTCAA 
CAGGTGGAGG TGAACACAGC CATGCATGAA GCCAAGCTGG TGGAGGAGTG TGATGAATTG 
GTGGAGATAA TTCGCCAGAG GAAACAGGTC ATTGCTGTCA AGATCAAAGA GTCCAAGGTG 
ATGAAGCTGA GGAAGTTGGC TCAGCAGATT GCGAACTGCA GACAGTGTCT GGAGCGCTCG 
AGCGCCCTCA TCACACAGGC GGAGCACAGC CTGAAGGAGA ACGACCACAC CCGATTCCTC 
CAGACGGCCA GGAATGTGGC GGAGAGAGTT GCCATGGCCA CCGCCTCCTC CCAGGTCCTC 
ATCCCAGACG TCAATCTGAA CGAGGCCTTT GACAATTTTG CCCTTGATTT CTCCAGAGAA 
AAGAAAATCC TGGAAGGACT GGATTATCTG ACAGCTCCCA ACCCTCCCTC GATACGCGAA 
GAGCTCTGCA CGGCCTCTCA CGACACCATT ACAGTGCACT GGACATCTGA AGATGAATTC 
AGCGTCACGT CTTATGAGCT TCAGTACACC ATCTACACTG GACAGACCAA CTTCATCAGT 
CTATATAACT CTGTGGATAG TTGGATGATT GTACCCAATA TCAAGCAGAA TCACTACACA 
GTCCACGGCC TGCAGAGCGG GACTCGTTAC ATCTTTTTCG TCAAGGCCAT CAACCAGGCA 
GGAAGCAGAA ACAGTGAACC CGCTCGCCTC AAAACCAACA GTCAGCCTTT CAAACTAGAC 
CCCAAAACGG CCCATAAGAA ACTGCGGCTC TCGAATGACT GTCTGACAAT GGAGAAGGAT 
GAAAGCTCGC TGAAGAAAAG CCACACTCCA GAACGCTTCA GCGGAACTGG ATCTTATGGA 
GCTGCTGGCA ACGTCTTCAT TGACAGTGGT TGCCATTATT GGGAAGTACT ACTGGGAGCC 
TCTACATGGT ACGCTCTTGG TGTGGCCTAT AAGTCAGCAC CCAAAAACGA GTGGAGTGGT 
AAAAACTCAT CATCTTGGGT TTTCTCACGC TGCAACAATA ACTTCTTGGT GCGGCACAAC 
AACAAGGAGT CCATCGTGGA AGCGTCACTA CAGCTGCGCA GGGTGGGAGT TCTTCTGGAC 
TACGACAACA ACACTCTGTC CTTCTATGAT GCCATGAACT CCCAGCACAT ATACACTTTT 
GACGTTTCCT TCCTGCTTCC GGTGGCTCCC ACCTTCATGA TCTGGAACAA GTCATTGATG 
ATCATGTCAG GGCTGCCGGT GCCTGACTTT GTGGACTGCA GCGAGCAGCA AGAGGGCATC 
TGCCGCCAAC AGGAGTCACC CTATGTTTCT GGAATAAAGA GCTGCCACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mid2 ( XM_009291077.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009289352.1 Danio rerio midline 2 (mid2), transcript variant X5, mRNA.

Price & Availability↑

CloneID	ODa47436
Clone ID Related Accession (Same CDS sequence)	XM_009291076.2 , XM_009291077.2 , XM_009291077.3 , XM_009291076.3
Accession Version	XM_009291077.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2091bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	probable E3 ubiquitin-protein ligase MID2 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007125.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009291077.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAACCC TGGAGTCCGA GCTGACGTGC CCGATCTGCC TGGAACTGTT TGAGGACCCG 
CTCCTGCTCC CCTGCGCCCA CAGCCTGTGC TTCAACTGCG CCCACCGCAT CTTGGTGTCC 
CACTGCTCAA GCACCAAACC CCTCGAGTCC ATCTCTGCCT TCCAGTGCCC CACCTGCCGC 
TACGTCATCA CGCTTAACCA GCGCGGCCTG GAGGGCCTAA AGCGCAATGT GACACTGCAG 
AACATCATCG ACCGCTTCCA GAAGGCCTCT GTCAGCGGGC CTAATTCTCC AAACGAGAGT 
CGCAGGCCCC GGCCCTACAG CGCCACGCTA GGCGGGAGCG CGAGGGGCAG CCCCGCCAGC 
AGCGCCATGT CCCTCGAGCG TGTCGGGTGT CAGTTCTGTG ACCAGGAGCC TCCGCGCGAG 
GCGGTGAAGA CATGTGTGAC GTGCGAGGTG TCATACTGCG ACCGCTGCCT GAGGGCCACA 
CACCCCAATA AGAAGCCCTT CACCAGCCAT CGTCTGGTGG AGCCAGTGGC GGATGCTCAG 
CTGCGTGGAC TAGCCTGTTT GGAGCATGAA AACGAGAAGG TCAACATGTA CTGTGTGGTT 
GACGATCAGC TCATCTGCGC CCTTTGCAAG TTGGTGGGAC GCCACCGTGA ACATCAGGTC 
ACCTCGCTGA GCGATCGCTT CGACAAGCTG AAGCAAACCT TGGAGAATAA CTTAACCAAT 
CTGGTGAAGA GGAACAGTGA GCTGGAGAAC CAGATGGCCA AACTCATCCA GATTTGTCAA 
CAGGTGGAGG TGAACACAGC CATGCATGAA GCCAAGCTGG TGGAGGAGTG TGATGAATTG 
GTGGAGATAA TTCGCCAGAG GAAACAGGTC ATTGCTGTCA AGATCAAAGA GTCCAAGGTG 
ATGAAGCTGA GGAAGTTGGC TCAGCAGATT GCGAACTGCA GACAGTGTCT GGAGCGCTCG 
AGCGCCCTCA TCACACAGGC GGAGCACAGC CTGAAGGAGA ACGACCACAC CCGATTCCTC 
CAGACGGCCA GGAATGTGGC GGAGAGAGTT GCCATGGCCA CCGCCTCCTC CCAGGTCCTC 
ATCCCAGACG TCAATCTGAA CGAGGCCTTT GACAATTTTG CCCTTGATTT CTCCAGAGAA 
AAGAAAATCC TGGAAGGACT GGATTATCTG ACAGCTCCCA ACCCTCCCTC GATACGCGAA 
GAGCTCTGCA CGGCCTCTCA CGACACCATT ACAGTGCACT GGACATCTGA AGATGAATTC 
AGCGTCACGT CTTATGAGCT TCAGTACACC ATCTACACTG GACAGACCAA CTTCATCAGT 
CTATATAACT CTGTGGATAG TTGGATGATT GTACCCAATA TCAAGCAGAA TCACTACACA 
GTCCACGGCC TGCAGAGCGG GACTCGTTAC ATCTTTTTCG TCAAGGCCAT CAACCAGGCA 
GGAAGCAGAA ACAGTGAACC CGCTCGCCTC AAAACCAACA GTCAGCCTTT CAAACTAGAC 
CCCAAAACGG CCCATAAGAA ACTGCGGCTC TCGAATGACT GTCTGACAAT GGAGAAGGAT 
GAAAGCTCGC TGAAGAAAAG CCACACTCCA GAACGCTTCA GCGGAACTGG ATCTTATGGA 
GCTGCTGGCA ACGTCTTCAT TGACAGTGGT TGCCATTATT GGGAAGTACT ACTGGGAGCC 
TCTACATGGT ACGCTCTTGG TGTGGCCTAT AAGTCAGCAC CCAAAAACGA GTGGAGTGGT 
AAAAACTCAT CATCTTGGGT TTTCTCACGC TGCAACAATA ACTTCTTGGT GCGGCACAAC 
AACAAGGAGT CCATCGTGGA AGCGTCACTA CAGCTGCGCA GGGTGGGAGT TCTTCTGGAC 
TACGACAACA ACACTCTGTC CTTCTATGAT GCCATGAACT CCCAGCACAT ATACACTTTT 
GACGTTTCCT TCCTGCTTCC GGTGGCTCCC ACCTTCATGA TCTGGAACAA GTCATTGATG 
ATCATGTCAG GGCTGCCGGT GCCTGACTTT GTGGACTGCA GCGAGCAGCA AGAGGGCATC 
TGCCGCCAAC AGGAGTCACC CTATGTTTCT GGAATAAAGA GCTGCCACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009289352.1
CDS	130..2220
Translation	METLESELTCPICLELFEDPLLLPCAHSLCFNCAHRILVSHCSSTKPLESISAFQCPTCRYVITLNQRGLEGLKRNVTLQNIIDRFQKASVSGPNSPNESRRPRPYSATLGGSARGSPASSAMSLERVGCQFCDQEPPREAVKTCVTCEVSYCDRCLRATHPNKKPFTSHRLVEPVADAQLRGLACLEHENEKVNMYCVVDDQLICALCKLVGRHREHQVTSLSDRFDKLKQTLENNLTNLVKRNSELENQMAKLIQICQQVEVNTAMHEAKLVEECDELVEIIRQRKQVIAVKIKESKVMKLRKLAQQIANCRQCLERSSALITQAEHSLKENDHTRFLQTARNVAERVAMATASSQVLIPDVNLNEAFDNFALDFSREKKILEGLDYLTAPNPPSIREELCTASHDTITVHWTSEDEFSVTSYELQYTIYTGQTNFISLYNSVDSWMIVPNIKQNHYTVHGLQSGTRYIFFVKAINQAGSRNSEPARLKTNSQPFKLDPKTAHKKLRLSNDCLTMEKDESSLKKSHTPERFSGTGSYGAAGNVFIDSGCHYWEVLLGASTWYALGVAYKSAPKNEWSGKNSSSWVFSRCNNNFLVRHNNKESIVEASLQLRRVGVLLDYDNNTLSFYDAMNSQHIYTFDVSFLLPVAPTFMIWNKSLMIMSGLPVPDFVDCSEQQEGICRQQESPYVSGIKSCH

Target ORF information:

RefSeq Version	XM_009291077.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio midline 2 (mid2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009291077.2

ORF Insert Sequence:

ATGGAAACCC TGGAGTCCGA GCTGACGTGC CCGATCTGCC TGGAACTGTT TGAGGACCCG 
CTCCTGCTCC CCTGCGCCCA CAGCCTGTGC TTCAACTGCG CCCACCGCAT CTTGGTGTCC 
CACTGCTCAA GCACCAAACC CCTCGAGTCC ATCTCTGCCT TCCAGTGCCC CACCTGCCGC 
TACGTCATCA CGCTTAACCA GCGCGGCCTG GAGGGCCTAA AGCGCAATGT GACACTGCAG 
AACATCATCG ACCGCTTCCA GAAGGCCTCT GTCAGCGGGC CTAATTCTCC AAACGAGAGT 
CGCAGGCCCC GGCCCTACAG CGCCACGCTA GGCGGGAGCG CGAGGGGCAG CCCCGCCAGC 
AGCGCCATGT CCCTCGAGCG TGTCGGGTGT CAGTTCTGTG ACCAGGAGCC TCCGCGCGAG 
GCGGTGAAGA CATGTGTGAC GTGCGAGGTG TCATACTGCG ACCGCTGCCT GAGGGCCACA 
CACCCCAATA AGAAGCCCTT CACCAGCCAT CGTCTGGTGG AGCCAGTGGC GGATGCTCAG 
CTGCGTGGAC TAGCCTGTTT GGAGCATGAA AACGAGAAGG TCAACATGTA CTGTGTGGTT 
GACGATCAGC TCATCTGCGC CCTTTGCAAG TTGGTGGGAC GCCACCGTGA ACATCAGGTC 
ACCTCGCTGA GCGATCGCTT CGACAAGCTG AAGCAAACCT TGGAGAATAA CTTAACCAAT 
CTGGTGAAGA GGAACAGTGA GCTGGAGAAC CAGATGGCCA AACTCATCCA GATTTGTCAA 
CAGGTGGAGG TGAACACAGC CATGCATGAA GCCAAGCTGG TGGAGGAGTG TGATGAATTG 
GTGGAGATAA TTCGCCAGAG GAAACAGGTC ATTGCTGTCA AGATCAAAGA GTCCAAGGTG 
ATGAAGCTGA GGAAGTTGGC TCAGCAGATT GCGAACTGCA GACAGTGTCT GGAGCGCTCG 
AGCGCCCTCA TCACACAGGC GGAGCACAGC CTGAAGGAGA ACGACCACAC CCGATTCCTC 
CAGACGGCCA GGAATGTGGC GGAGAGAGTT GCCATGGCCA CCGCCTCCTC CCAGGTCCTC 
ATCCCAGACG TCAATCTGAA CGAGGCCTTT GACAATTTTG CCCTTGATTT CTCCAGAGAA 
AAGAAAATCC TGGAAGGACT GGATTATCTG ACAGCTCCCA ACCCTCCCTC GATACGCGAA 
GAGCTCTGCA CGGCCTCTCA CGACACCATT ACAGTGCACT GGACATCTGA AGATGAATTC 
AGCGTCACGT CTTATGAGCT TCAGTACACC ATCTACACTG GACAGACCAA CTTCATCAGT 
CTATATAACT CTGTGGATAG TTGGATGATT GTACCCAATA TCAAGCAGAA TCACTACACA 
GTCCACGGCC TGCAGAGCGG GACTCGTTAC ATCTTTTTCG TCAAGGCCAT CAACCAGGCA 
GGAAGCAGAA ACAGTGAACC CGCTCGCCTC AAAACCAACA GTCAGCCTTT CAAACTAGAC 
CCCAAAACGG CCCATAAGAA ACTGCGGCTC TCGAATGACT GTCTGACAAT GGAGAAGGAT 
GAAAGCTCGC TGAAGAAAAG CCACACTCCA GAACGCTTCA GCGGAACTGG ATCTTATGGA 
GCTGCTGGCA ACGTCTTCAT TGACAGTGGT TGCCATTATT GGGAAGTACT ACTGGGAGCC 
TCTACATGGT ACGCTCTTGG TGTGGCCTAT AAGTCAGCAC CCAAAAACGA GTGGAGTGGT 
AAAAACTCAT CATCTTGGGT TTTCTCACGC TGCAACAATA ACTTCTTGGT GCGGCACAAC 
AACAAGGAGT CCATCGTGGA AGCGTCACTA CAGCTGCGCA GGGTGGGAGT TCTTCTGGAC 
TACGACAACA ACACTCTGTC CTTCTATGAT GCCATGAACT CCCAGCACAT ATACACTTTT 
GACGTTTCCT TCCTGCTTCC GGTGGCTCCC ACCTTCATGA TCTGGAACAA GTCATTGATG 
ATCATGTCAG GGCTGCCGGT GCCTGACTTT GTGGACTGCA GCGAGCAGCA AGAGGGCATC 
TGCCGCCAAC AGGAGTCACC CTATGTTTCT GGAATAAAGA GCTGCCACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mid2 ( XM_009291077.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009289352.1 Danio rerio midline 2 (mid2), transcript variant X6, mRNA.

Price & Availability↑

CloneID	ODa47436
Clone ID Related Accession (Same CDS sequence)	XM_009291076.2 , XM_009291077.2 , XM_009291077.3 , XM_009291076.3
Accession Version	XM_009291077.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2091bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	probable E3 ubiquitin-protein ligase MID2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007125.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009291077.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAACCC TGGAGTCCGA GCTGACGTGC CCGATCTGCC TGGAACTGTT TGAGGACCCG 
CTCCTGCTCC CCTGCGCCCA CAGCCTGTGC TTCAACTGCG CCCACCGCAT CTTGGTGTCC 
CACTGCTCAA GCACCAAACC CCTCGAGTCC ATCTCTGCCT TCCAGTGCCC CACCTGCCGC 
TACGTCATCA CGCTTAACCA GCGCGGCCTG GAGGGCCTAA AGCGCAATGT GACACTGCAG 
AACATCATCG ACCGCTTCCA GAAGGCCTCT GTCAGCGGGC CTAATTCTCC AAACGAGAGT 
CGCAGGCCCC GGCCCTACAG CGCCACGCTA GGCGGGAGCG CGAGGGGCAG CCCCGCCAGC 
AGCGCCATGT CCCTCGAGCG TGTCGGGTGT CAGTTCTGTG ACCAGGAGCC TCCGCGCGAG 
GCGGTGAAGA CATGTGTGAC GTGCGAGGTG TCATACTGCG ACCGCTGCCT GAGGGCCACA 
CACCCCAATA AGAAGCCCTT CACCAGCCAT CGTCTGGTGG AGCCAGTGGC GGATGCTCAG 
CTGCGTGGAC TAGCCTGTTT GGAGCATGAA AACGAGAAGG TCAACATGTA CTGTGTGGTT 
GACGATCAGC TCATCTGCGC CCTTTGCAAG TTGGTGGGAC GCCACCGTGA ACATCAGGTC 
ACCTCGCTGA GCGATCGCTT CGACAAGCTG AAGCAAACCT TGGAGAATAA CTTAACCAAT 
CTGGTGAAGA GGAACAGTGA GCTGGAGAAC CAGATGGCCA AACTCATCCA GATTTGTCAA 
CAGGTGGAGG TGAACACAGC CATGCATGAA GCCAAGCTGG TGGAGGAGTG TGATGAATTG 
GTGGAGATAA TTCGCCAGAG GAAACAGGTC ATTGCTGTCA AGATCAAAGA GTCCAAGGTG 
ATGAAGCTGA GGAAGTTGGC TCAGCAGATT GCGAACTGCA GACAGTGTCT GGAGCGCTCG 
AGCGCCCTCA TCACACAGGC GGAGCACAGC CTGAAGGAGA ACGACCACAC CCGATTCCTC 
CAGACGGCCA GGAATGTGGC GGAGAGAGTT GCCATGGCCA CCGCCTCCTC CCAGGTCCTC 
ATCCCAGACG TCAATCTGAA CGAGGCCTTT GACAATTTTG CCCTTGATTT CTCCAGAGAA 
AAGAAAATCC TGGAAGGACT GGATTATCTG ACAGCTCCCA ACCCTCCCTC GATACGCGAA 
GAGCTCTGCA CGGCCTCTCA CGACACCATT ACAGTGCACT GGACATCTGA AGATGAATTC 
AGCGTCACGT CTTATGAGCT TCAGTACACC ATCTACACTG GACAGACCAA CTTCATCAGT 
CTATATAACT CTGTGGATAG TTGGATGATT GTACCCAATA TCAAGCAGAA TCACTACACA 
GTCCACGGCC TGCAGAGCGG GACTCGTTAC ATCTTTTTCG TCAAGGCCAT CAACCAGGCA 
GGAAGCAGAA ACAGTGAACC CGCTCGCCTC AAAACCAACA GTCAGCCTTT CAAACTAGAC 
CCCAAAACGG CCCATAAGAA ACTGCGGCTC TCGAATGACT GTCTGACAAT GGAGAAGGAT 
GAAAGCTCGC TGAAGAAAAG CCACACTCCA GAACGCTTCA GCGGAACTGG ATCTTATGGA 
GCTGCTGGCA ACGTCTTCAT TGACAGTGGT TGCCATTATT GGGAAGTACT ACTGGGAGCC 
TCTACATGGT ACGCTCTTGG TGTGGCCTAT AAGTCAGCAC CCAAAAACGA GTGGAGTGGT 
AAAAACTCAT CATCTTGGGT TTTCTCACGC TGCAACAATA ACTTCTTGGT GCGGCACAAC 
AACAAGGAGT CCATCGTGGA AGCGTCACTA CAGCTGCGCA GGGTGGGAGT TCTTCTGGAC 
TACGACAACA ACACTCTGTC CTTCTATGAT GCCATGAACT CCCAGCACAT ATACACTTTT 
GACGTTTCCT TCCTGCTTCC GGTGGCTCCC ACCTTCATGA TCTGGAACAA GTCATTGATG 
ATCATGTCAG GGCTGCCGGT GCCTGACTTT GTGGACTGCA GCGAGCAGCA AGAGGGCATC 
TGCCGCCAAC AGGAGTCACC CTATGTTTCT GGAATAAAGA GCTGCCACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009289352.1
CDS	133..2223
Translation	METLESELTCPICLELFEDPLLLPCAHSLCFNCAHRILVSHCSSTKPLESISAFQCPTCRYVITLNQRGLEGLKRNVTLQNIIDRFQKASVSGPNSPNESRRPRPYSATLGGSARGSPASSAMSLERVGCQFCDQEPPREAVKTCVTCEVSYCDRCLRATHPNKKPFTSHRLVEPVADAQLRGLACLEHENEKVNMYCVVDDQLICALCKLVGRHREHQVTSLSDRFDKLKQTLENNLTNLVKRNSELENQMAKLIQICQQVEVNTAMHEAKLVEECDELVEIIRQRKQVIAVKIKESKVMKLRKLAQQIANCRQCLERSSALITQAEHSLKENDHTRFLQTARNVAERVAMATASSQVLIPDVNLNEAFDNFALDFSREKKILEGLDYLTAPNPPSIREELCTASHDTITVHWTSEDEFSVTSYELQYTIYTGQTNFISLYNSVDSWMIVPNIKQNHYTVHGLQSGTRYIFFVKAINQAGSRNSEPARLKTNSQPFKLDPKTAHKKLRLSNDCLTMEKDESSLKKSHTPERFSGTGSYGAAGNVFIDSGCHYWEVLLGASTWYALGVAYKSAPKNEWSGKNSSSWVFSRCNNNFLVRHNNKESIVEASLQLRRVGVLLDYDNNTLSFYDAMNSQHIYTFDVSFLLPVAPTFMIWNKSLMIMSGLPVPDFVDCSEQQEGICRQQESPYVSGIKSCH

Target ORF information:

RefSeq Version	XM_009291077.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio midline 2 (mid2), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009291077.3

ORF Insert Sequence:

ATGGAAACCC TGGAGTCCGA GCTGACGTGC CCGATCTGCC TGGAACTGTT TGAGGACCCG 
CTCCTGCTCC CCTGCGCCCA CAGCCTGTGC TTCAACTGCG CCCACCGCAT CTTGGTGTCC 
CACTGCTCAA GCACCAAACC CCTCGAGTCC ATCTCTGCCT TCCAGTGCCC CACCTGCCGC 
TACGTCATCA CGCTTAACCA GCGCGGCCTG GAGGGCCTAA AGCGCAATGT GACACTGCAG 
AACATCATCG ACCGCTTCCA GAAGGCCTCT GTCAGCGGGC CTAATTCTCC AAACGAGAGT 
CGCAGGCCCC GGCCCTACAG CGCCACGCTA GGCGGGAGCG CGAGGGGCAG CCCCGCCAGC 
AGCGCCATGT CCCTCGAGCG TGTCGGGTGT CAGTTCTGTG ACCAGGAGCC TCCGCGCGAG 
GCGGTGAAGA CATGTGTGAC GTGCGAGGTG TCATACTGCG ACCGCTGCCT GAGGGCCACA 
CACCCCAATA AGAAGCCCTT CACCAGCCAT CGTCTGGTGG AGCCAGTGGC GGATGCTCAG 
CTGCGTGGAC TAGCCTGTTT GGAGCATGAA AACGAGAAGG TCAACATGTA CTGTGTGGTT 
GACGATCAGC TCATCTGCGC CCTTTGCAAG TTGGTGGGAC GCCACCGTGA ACATCAGGTC 
ACCTCGCTGA GCGATCGCTT CGACAAGCTG AAGCAAACCT TGGAGAATAA CTTAACCAAT 
CTGGTGAAGA GGAACAGTGA GCTGGAGAAC CAGATGGCCA AACTCATCCA GATTTGTCAA 
CAGGTGGAGG TGAACACAGC CATGCATGAA GCCAAGCTGG TGGAGGAGTG TGATGAATTG 
GTGGAGATAA TTCGCCAGAG GAAACAGGTC ATTGCTGTCA AGATCAAAGA GTCCAAGGTG 
ATGAAGCTGA GGAAGTTGGC TCAGCAGATT GCGAACTGCA GACAGTGTCT GGAGCGCTCG 
AGCGCCCTCA TCACACAGGC GGAGCACAGC CTGAAGGAGA ACGACCACAC CCGATTCCTC 
CAGACGGCCA GGAATGTGGC GGAGAGAGTT GCCATGGCCA CCGCCTCCTC CCAGGTCCTC 
ATCCCAGACG TCAATCTGAA CGAGGCCTTT GACAATTTTG CCCTTGATTT CTCCAGAGAA 
AAGAAAATCC TGGAAGGACT GGATTATCTG ACAGCTCCCA ACCCTCCCTC GATACGCGAA 
GAGCTCTGCA CGGCCTCTCA CGACACCATT ACAGTGCACT GGACATCTGA AGATGAATTC 
AGCGTCACGT CTTATGAGCT TCAGTACACC ATCTACACTG GACAGACCAA CTTCATCAGT 
CTATATAACT CTGTGGATAG TTGGATGATT GTACCCAATA TCAAGCAGAA TCACTACACA 
GTCCACGGCC TGCAGAGCGG GACTCGTTAC ATCTTTTTCG TCAAGGCCAT CAACCAGGCA 
GGAAGCAGAA ACAGTGAACC CGCTCGCCTC AAAACCAACA GTCAGCCTTT CAAACTAGAC 
CCCAAAACGG CCCATAAGAA ACTGCGGCTC TCGAATGACT GTCTGACAAT GGAGAAGGAT 
GAAAGCTCGC TGAAGAAAAG CCACACTCCA GAACGCTTCA GCGGAACTGG ATCTTATGGA 
GCTGCTGGCA ACGTCTTCAT TGACAGTGGT TGCCATTATT GGGAAGTACT ACTGGGAGCC 
TCTACATGGT ACGCTCTTGG TGTGGCCTAT AAGTCAGCAC CCAAAAACGA GTGGAGTGGT 
AAAAACTCAT CATCTTGGGT TTTCTCACGC TGCAACAATA ACTTCTTGGT GCGGCACAAC 
AACAAGGAGT CCATCGTGGA AGCGTCACTA CAGCTGCGCA GGGTGGGAGT TCTTCTGGAC 
TACGACAACA ACACTCTGTC CTTCTATGAT GCCATGAACT CCCAGCACAT ATACACTTTT 
GACGTTTCCT TCCTGCTTCC GGTGGCTCCC ACCTTCATGA TCTGGAACAA GTCATTGATG 
ATCATGTCAG GGCTGCCGGT GCCTGACTTT GTGGACTGCA GCGAGCAGCA AGAGGGCATC 
TGCCGCCAAC AGGAGTCACC CTATGTTTCT GGAATAAAGA GCTGCCACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mid2 ( XM_009291076.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009289351.1 Danio rerio midline 2 (mid2), transcript variant X5, mRNA.

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CloneID	ODa47436
Clone ID Related Accession (Same CDS sequence)	XM_009291076.2 , XM_009291077.2 , XM_009291077.3 , XM_009291076.3
Accession Version	XM_009291076.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2091bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	probable E3 ubiquitin-protein ligase MID2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007125.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009291076.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAACCC TGGAGTCCGA GCTGACGTGC CCGATCTGCC TGGAACTGTT TGAGGACCCG 
CTCCTGCTCC CCTGCGCCCA CAGCCTGTGC TTCAACTGCG CCCACCGCAT CTTGGTGTCC 
CACTGCTCAA GCACCAAACC CCTCGAGTCC ATCTCTGCCT TCCAGTGCCC CACCTGCCGC 
TACGTCATCA CGCTTAACCA GCGCGGCCTG GAGGGCCTAA AGCGCAATGT GACACTGCAG 
AACATCATCG ACCGCTTCCA GAAGGCCTCT GTCAGCGGGC CTAATTCTCC AAACGAGAGT 
CGCAGGCCCC GGCCCTACAG CGCCACGCTA GGCGGGAGCG CGAGGGGCAG CCCCGCCAGC 
AGCGCCATGT CCCTCGAGCG TGTCGGGTGT CAGTTCTGTG ACCAGGAGCC TCCGCGCGAG 
GCGGTGAAGA CATGTGTGAC GTGCGAGGTG TCATACTGCG ACCGCTGCCT GAGGGCCACA 
CACCCCAATA AGAAGCCCTT CACCAGCCAT CGTCTGGTGG AGCCAGTGGC GGATGCTCAG 
CTGCGTGGAC TAGCCTGTTT GGAGCATGAA AACGAGAAGG TCAACATGTA CTGTGTGGTT 
GACGATCAGC TCATCTGCGC CCTTTGCAAG TTGGTGGGAC GCCACCGTGA ACATCAGGTC 
ACCTCGCTGA GCGATCGCTT CGACAAGCTG AAGCAAACCT TGGAGAATAA CTTAACCAAT 
CTGGTGAAGA GGAACAGTGA GCTGGAGAAC CAGATGGCCA AACTCATCCA GATTTGTCAA 
CAGGTGGAGG TGAACACAGC CATGCATGAA GCCAAGCTGG TGGAGGAGTG TGATGAATTG 
GTGGAGATAA TTCGCCAGAG GAAACAGGTC ATTGCTGTCA AGATCAAAGA GTCCAAGGTG 
ATGAAGCTGA GGAAGTTGGC TCAGCAGATT GCGAACTGCA GACAGTGTCT GGAGCGCTCG 
AGCGCCCTCA TCACACAGGC GGAGCACAGC CTGAAGGAGA ACGACCACAC CCGATTCCTC 
CAGACGGCCA GGAATGTGGC GGAGAGAGTT GCCATGGCCA CCGCCTCCTC CCAGGTCCTC 
ATCCCAGACG TCAATCTGAA CGAGGCCTTT GACAATTTTG CCCTTGATTT CTCCAGAGAA 
AAGAAAATCC TGGAAGGACT GGATTATCTG ACAGCTCCCA ACCCTCCCTC GATACGCGAA 
GAGCTCTGCA CGGCCTCTCA CGACACCATT ACAGTGCACT GGACATCTGA AGATGAATTC 
AGCGTCACGT CTTATGAGCT TCAGTACACC ATCTACACTG GACAGACCAA CTTCATCAGT 
CTATATAACT CTGTGGATAG TTGGATGATT GTACCCAATA TCAAGCAGAA TCACTACACA 
GTCCACGGCC TGCAGAGCGG GACTCGTTAC ATCTTTTTCG TCAAGGCCAT CAACCAGGCA 
GGAAGCAGAA ACAGTGAACC CGCTCGCCTC AAAACCAACA GTCAGCCTTT CAAACTAGAC 
CCCAAAACGG CCCATAAGAA ACTGCGGCTC TCGAATGACT GTCTGACAAT GGAGAAGGAT 
GAAAGCTCGC TGAAGAAAAG CCACACTCCA GAACGCTTCA GCGGAACTGG ATCTTATGGA 
GCTGCTGGCA ACGTCTTCAT TGACAGTGGT TGCCATTATT GGGAAGTACT ACTGGGAGCC 
TCTACATGGT ACGCTCTTGG TGTGGCCTAT AAGTCAGCAC CCAAAAACGA GTGGAGTGGT 
AAAAACTCAT CATCTTGGGT TTTCTCACGC TGCAACAATA ACTTCTTGGT GCGGCACAAC 
AACAAGGAGT CCATCGTGGA AGCGTCACTA CAGCTGCGCA GGGTGGGAGT TCTTCTGGAC 
TACGACAACA ACACTCTGTC CTTCTATGAT GCCATGAACT CCCAGCACAT ATACACTTTT 
GACGTTTCCT TCCTGCTTCC GGTGGCTCCC ACCTTCATGA TCTGGAACAA GTCATTGATG 
ATCATGTCAG GGCTGCCGGT GCCTGACTTT GTGGACTGCA GCGAGCAGCA AGAGGGCATC 
TGCCGCCAAC AGGAGTCACC CTATGTTTCT GGAATAAAGA GCTGCCACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009289351.1
CDS	257..2347
Translation	METLESELTCPICLELFEDPLLLPCAHSLCFNCAHRILVSHCSSTKPLESISAFQCPTCRYVITLNQRGLEGLKRNVTLQNIIDRFQKASVSGPNSPNESRRPRPYSATLGGSARGSPASSAMSLERVGCQFCDQEPPREAVKTCVTCEVSYCDRCLRATHPNKKPFTSHRLVEPVADAQLRGLACLEHENEKVNMYCVVDDQLICALCKLVGRHREHQVTSLSDRFDKLKQTLENNLTNLVKRNSELENQMAKLIQICQQVEVNTAMHEAKLVEECDELVEIIRQRKQVIAVKIKESKVMKLRKLAQQIANCRQCLERSSALITQAEHSLKENDHTRFLQTARNVAERVAMATASSQVLIPDVNLNEAFDNFALDFSREKKILEGLDYLTAPNPPSIREELCTASHDTITVHWTSEDEFSVTSYELQYTIYTGQTNFISLYNSVDSWMIVPNIKQNHYTVHGLQSGTRYIFFVKAINQAGSRNSEPARLKTNSQPFKLDPKTAHKKLRLSNDCLTMEKDESSLKKSHTPERFSGTGSYGAAGNVFIDSGCHYWEVLLGASTWYALGVAYKSAPKNEWSGKNSSSWVFSRCNNNFLVRHNNKESIVEASLQLRRVGVLLDYDNNTLSFYDAMNSQHIYTFDVSFLLPVAPTFMIWNKSLMIMSGLPVPDFVDCSEQQEGICRQQESPYVSGIKSCH

Target ORF information:

RefSeq Version	XM_009291076.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio midline 2 (mid2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009291076.3

ORF Insert Sequence:

ATGGAAACCC TGGAGTCCGA GCTGACGTGC CCGATCTGCC TGGAACTGTT TGAGGACCCG 
CTCCTGCTCC CCTGCGCCCA CAGCCTGTGC TTCAACTGCG CCCACCGCAT CTTGGTGTCC 
CACTGCTCAA GCACCAAACC CCTCGAGTCC ATCTCTGCCT TCCAGTGCCC CACCTGCCGC 
TACGTCATCA CGCTTAACCA GCGCGGCCTG GAGGGCCTAA AGCGCAATGT GACACTGCAG 
AACATCATCG ACCGCTTCCA GAAGGCCTCT GTCAGCGGGC CTAATTCTCC AAACGAGAGT 
CGCAGGCCCC GGCCCTACAG CGCCACGCTA GGCGGGAGCG CGAGGGGCAG CCCCGCCAGC 
AGCGCCATGT CCCTCGAGCG TGTCGGGTGT CAGTTCTGTG ACCAGGAGCC TCCGCGCGAG 
GCGGTGAAGA CATGTGTGAC GTGCGAGGTG TCATACTGCG ACCGCTGCCT GAGGGCCACA 
CACCCCAATA AGAAGCCCTT CACCAGCCAT CGTCTGGTGG AGCCAGTGGC GGATGCTCAG 
CTGCGTGGAC TAGCCTGTTT GGAGCATGAA AACGAGAAGG TCAACATGTA CTGTGTGGTT 
GACGATCAGC TCATCTGCGC CCTTTGCAAG TTGGTGGGAC GCCACCGTGA ACATCAGGTC 
ACCTCGCTGA GCGATCGCTT CGACAAGCTG AAGCAAACCT TGGAGAATAA CTTAACCAAT 
CTGGTGAAGA GGAACAGTGA GCTGGAGAAC CAGATGGCCA AACTCATCCA GATTTGTCAA 
CAGGTGGAGG TGAACACAGC CATGCATGAA GCCAAGCTGG TGGAGGAGTG TGATGAATTG 
GTGGAGATAA TTCGCCAGAG GAAACAGGTC ATTGCTGTCA AGATCAAAGA GTCCAAGGTG 
ATGAAGCTGA GGAAGTTGGC TCAGCAGATT GCGAACTGCA GACAGTGTCT GGAGCGCTCG 
AGCGCCCTCA TCACACAGGC GGAGCACAGC CTGAAGGAGA ACGACCACAC CCGATTCCTC 
CAGACGGCCA GGAATGTGGC GGAGAGAGTT GCCATGGCCA CCGCCTCCTC CCAGGTCCTC 
ATCCCAGACG TCAATCTGAA CGAGGCCTTT GACAATTTTG CCCTTGATTT CTCCAGAGAA 
AAGAAAATCC TGGAAGGACT GGATTATCTG ACAGCTCCCA ACCCTCCCTC GATACGCGAA 
GAGCTCTGCA CGGCCTCTCA CGACACCATT ACAGTGCACT GGACATCTGA AGATGAATTC 
AGCGTCACGT CTTATGAGCT TCAGTACACC ATCTACACTG GACAGACCAA CTTCATCAGT 
CTATATAACT CTGTGGATAG TTGGATGATT GTACCCAATA TCAAGCAGAA TCACTACACA 
GTCCACGGCC TGCAGAGCGG GACTCGTTAC ATCTTTTTCG TCAAGGCCAT CAACCAGGCA 
GGAAGCAGAA ACAGTGAACC CGCTCGCCTC AAAACCAACA GTCAGCCTTT CAAACTAGAC 
CCCAAAACGG CCCATAAGAA ACTGCGGCTC TCGAATGACT GTCTGACAAT GGAGAAGGAT 
GAAAGCTCGC TGAAGAAAAG CCACACTCCA GAACGCTTCA GCGGAACTGG ATCTTATGGA 
GCTGCTGGCA ACGTCTTCAT TGACAGTGGT TGCCATTATT GGGAAGTACT ACTGGGAGCC 
TCTACATGGT ACGCTCTTGG TGTGGCCTAT AAGTCAGCAC CCAAAAACGA GTGGAGTGGT 
AAAAACTCAT CATCTTGGGT TTTCTCACGC TGCAACAATA ACTTCTTGGT GCGGCACAAC 
AACAAGGAGT CCATCGTGGA AGCGTCACTA CAGCTGCGCA GGGTGGGAGT TCTTCTGGAC 
TACGACAACA ACACTCTGTC CTTCTATGAT GCCATGAACT CCCAGCACAT ATACACTTTT 
GACGTTTCCT TCCTGCTTCC GGTGGCTCCC ACCTTCATGA TCTGGAACAA GTCATTGATG 
ATCATGTCAG GGCTGCCGGT GCCTGACTTT GTGGACTGCA GCGAGCAGCA AGAGGGCATC 
TGCCGCCAAC AGGAGTCACC CTATGTTTCT GGAATAAAGA GCTGCCACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	mid2
Entrez Gene ID	799891
Full Name	midline 2
General protein information	Preferred Names midline 2 Names midline-2 probable E3 ubiquitin-protein ligase MID2 trim1
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

Bos taurus (cattle)	LOC616821	XP_002699930.2
Danio rerio (zebrafish)	mid2	XP_001340198.5

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

mid2 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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