SELENOF cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	SELENOF
Entrez Gene ID	614360
Full Name	selenoprotein F
Synonyms	SEP15
Gene Type	protein-coding
Organism	Bos taurus(cattle)
Summary	The protein encoded by this gene belongs to the SEP15/selenoprotein M family. The exact function of this protein is not known; however, it has been found to associate with UDP-glucose:glycoprotein glucosyltransferase (UGTR), an endoplasmic reticulum(ER)-resident protein, which is involved in the quality control of protein folding. The association with UGTR retains this protein in the ER, where it may play a role in protein folding. It has also been suggested to have a role in cancer etiology. This protein is a selenoprotein, containing the rare amino acid selenocysteine (Sec). Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal. [provided by RefSeq, Nov 2016].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001034759.2	NP_001029931.2	selenoprotein F precursor

Pathways from BioSystems

Homologies

	SEP15	NP_001152888.1
Bos taurus (cattle)	SEP15	NP_001029931.2
Gallus gallus (chicken)	SEP15	NP_001012944.2
Arabidopsis thaliana (thale cress)	AT1G05720	NP_563747.1
Xenopus tropicalis (tropical clawed frog)	sep15	NP_001165182.1
Homo sapiens (human)	SEP15	NP_004252.2
Pan troglodytes (chimpanzee)	SEP15	NP_001139315.1
Rattus norvegicus (Norway rat)	Sep15	NP_579831.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004986	XP_315090.2
Oryza sativa (rice)	Os01g0894500	NP_001045069.1
Canis lupus familiaris (dog)	SEP15	NP_001108232.1
Mus musculus (house mouse)	Sep15	NP_444332.1
Danio rerio (zebrafish)	sep15	NP_840079.1
Drosophila melanogaster (fruit fly)	CG7484	NP_649000.1
Caenorhabditis elegans (roundworm)	Y76B12C.3	NP_500152.1

Gene Ontology
Bibliography

Related articles in PubMed

Composition and evolution of the vertebrate and mammalian selenoproteomes.
Mariotti M, Ridge PG, Zhang Y, Lobanov AV, Pringle TH, Guigo R, Hatfield DL, Gladyshev VN
PloS one7(3)e33066(2012)

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

Characterization of 954 bovine full-CDS cDNA sequences.
Harhay GP, Sonstegard TS, Keele JW, Heaton MP, Clawson ML, Snelling WM, Wiedmann RT, Van Tassell CP, Smith TP
BMC genomics6166(2005 Nov)

Analysis of bovine mammary gland EST and functional annotation of the Bos taurus gene index.
Sonstegard TS, Capuco AV, White J, Van Tassell CP, Connor EE, Cho J, Sultana R, Shade L, Wray JE, Wells KD, Quackenbush J
Mammalian genome : official journal of the International Mammalian Genome Society13(7)373-9(2002 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following SELENOF gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SELENOF cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBa43872	NM_001034759.2 Latest version!	Bos taurus selenoprotein F (SELENOF), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SELENOF ( NM_001034759.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001029931.2 Bos taurus selenoprotein F (SELENOF), mRNA.

Price & Availability↑

CloneID	OBa43872
Clone ID Related Accession (Same CDS sequence)	NM_001034759.2
Accession Version	NM_001034759.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 489bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-19
Organism	Bos taurus(cattle)
Product	selenoprotein F precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DV841387.1, BT021503.1 and CN437037.1. On Apr 11, 2007 this sequence version replaced NM_001034759.1. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN03145412, SAMN03145413 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## protein contains selenocysteine :: inferred from conservation ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGGCCC GGCGGGATGG ATGGCTTGGC CCGGCGTTTG GGCTGCGACT GCTGCTGGCG 
ACCGTGCTTC AAACGGTCTC TGCATTGGGA GCAGAATTTT CCTCAGAGTC TTGCAGAGAG 
TTAGGTTTCT CCAGCAACTT GCTCTGCAGC TCCTGTGATT TGCTTGGACA GTTCAACCTT 
CTGCAGCTGG ATCCTGACTG TAGAGGGTGC TGTCAGGAAG AAGCACAATT TGAAACTAAA 
AAGCTGTATG CAGGAGCTAT TCTTGAAGTC TGTGGATGAA AATTGGGGAG GTTCCCTCAA 
GTCCAAGCTT TCGTCAGGAG TGATAAACCC AAGCTATTCA AAGGACTACA AATCAAGTAT 
GTTCGCGGTT CAGACCCTGT GCTAAAGCTT TTGGACGACA GTGGGAACAT TGCTGAAGAA 
CTAAGCATCC TCAAATGGAA CACAGACAGT GTAGAAGAAT TCCTGAGTGA AAAGTTGGAA 
CGCATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001029931.2
CDS	292..780
Translation	MAARRDGWLGPAFGLRLLLATVLQTVSALGAEFSSESCRELGFSSNLLCSSCDLLGQFNLLQLDPDCRGCCQEEAQFETKKLYAGAILEVCG*KLGRFPQVQAFVRSDKPKLFKGLQIKYVRGSDPVLKLLDDSGNIAEELSILKWNTDSVEEFLSEKLERI

Target ORF information:

RefSeq Version	NM_001034759.2
Organism	Bos taurus(cattle)
Definition	Bos taurus selenoprotein F (SELENOF), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001034759.2

ORF Insert Sequence:

ATGGCGGCCC GGCGGGATGG ATGGCTTGGC CCGGCGTTTG GGCTGCGACT GCTGCTGGCG 
ACCGTGCTTC AAACGGTCTC TGCATTGGGA GCAGAATTTT CCTCAGAGTC TTGCAGAGAG 
TTAGGTTTCT CCAGCAACTT GCTCTGCAGC TCCTGTGATT TGCTTGGACA GTTCAACCTT 
CTGCAGCTGG ATCCTGACTG TAGAGGGTGC TGTCAGGAAG AAGCACAATT TGAAACTAAA 
AAGCTGTATG CAGGAGCTAT TCTTGAAGTC TGTGGATGAA AATTGGGGAG GTTCCCTCAA 
GTCCAAGCTT TCGTCAGGAG TGATAAACCC AAGCTATTCA AAGGACTACA AATCAAGTAT 
GTTCGCGGTT CAGACCCTGT GCTAAAGCTT TTGGACGACA GTGGGAACAT TGCTGAAGAA 
CTAAGCATCC TCAAATGGAA CACAGACAGT GTAGAAGAAT TCCTGAGTGA AAAGTTGGAA 
CGCATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Composition and evolution of the vertebrate and mammalian selenoproteomes.
PloS one7(3)e33066(2012)
Mariotti M,Ridge PG,Zhang Y,Lobanov AV,Pringle TH,Guigo R,Hatfield DL,Gladyshev VN

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

Characterization of 954 bovine full-CDS cDNA sequences.
BMC genomics6166(2005 Nov)
Harhay GP,Sonstegard TS,Keele JW,Heaton MP,Clawson ML,Snelling WM,Wiedmann RT,Van Tassell CP,Smith TP

Analysis of bovine mammary gland EST and functional annotation of the Bos taurus gene index.
Mammalian genome : official journal of the International Mammalian Genome Society13(7)373-9(2002 Jul)
Sonstegard TS,Capuco AV,White J,Van Tassell CP,Connor EE,Cho J,Sultana R,Shade L,Wray JE,Wells KD,Quackenbush J

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SELENOF cDNA ORF clone, Bos taurus(cattle)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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