RIF1 gene cDNA ORF clone, Bos taurus(cattle)

mRNA	Protein	Name
XM_010802075.2	XP_010800377.1	telomere-associated protein RIF1 isoform X4
XM_010826492.2	XP_010824794.1	telomere-associated protein RIF1 isoform X1
XM_010826493.2	XP_010824795.1	telomere-associated protein RIF1 isoform X5
XM_010802073.3	XP_010800375.1	telomere-associated protein RIF1 isoform X2
XM_010802077.3	XP_010800379.1	telomere-associated protein RIF1 isoform X5
XM_010802073.2	XP_010800375.1	telomere-associated protein RIF1 isoform X2
XM_010802077.2	XP_010800379.1	telomere-associated protein RIF1 isoform X5
XM_010826489.2	XP_010824791.1	telomere-associated protein RIF1 isoform X2
XM_002685359.6	XP_002685405.1	telomere-associated protein RIF1 isoform X1
XM_010802074.3	XP_010800376.1	telomere-associated protein RIF1 isoform X3
XM_002685359.5	XP_002685405.1	telomere-associated protein RIF1 isoform X1
XM_010802075.3	XP_010800377.1	telomere-associated protein RIF1 isoform X4
XM_024980692.1	XP_024836460.1	telomere-associated protein RIF1 isoform X1
XM_024980695.1	XP_024836463.1	telomere-associated protein RIF1 isoform X1
XM_010802074.2	XP_010800376.1	telomere-associated protein RIF1 isoform X3
XM_010802076.2	XP_010800378.1	telomere-associated protein RIF1 isoform X1
XM_615269.9	XP_615269.3	telomere-associated protein RIF1 isoform X1
XM_010826490.2	XP_010824792.1	telomere-associated protein RIF1 isoform X3
XM_010826491.2	XP_010824793.1	telomere-associated protein RIF1 isoform X4

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa02875	XM_010802076.2 Latest version!	Bos taurus replication timing regulatory factor 1 (RIF1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OBa02875	XM_024980695.1 Latest version!	Bos taurus replication timing regulatory factor 1 (RIF1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OBa02875	XM_002685359.6 Latest version!	Bos taurus replication timing regulatory factor 1 (RIF1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OBa02875	XM_024980692.1 Latest version!	Bos taurus replication timing regulatory factor 1 (RIF1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OBa02875	XM_010826492.2 Latest version!	Bos taurus replication timing regulatory factor 1 (RIF1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OBa02875	XM_615269.9 Latest version!	Bos taurus replication timing regulatory factor 1 (RIF1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OBa55065	XM_010802073.3 Latest version!	Bos taurus replication timing regulatory factor 1 (RIF1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OBa55065	XM_010826489.2 Latest version!	Bos taurus replication timing regulatory factor 1 (RIF1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OBa55066	XM_010826490.2 Latest version!	Bos taurus replication timing regulatory factor 1 (RIF1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OBa55066	XM_010802074.3 Latest version!	Bos taurus replication timing regulatory factor 1 (RIF1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OBa55067	XM_010826491.2 Latest version!	Bos taurus replication timing regulatory factor 1 (RIF1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OBa55067	XM_010802075.3 Latest version!	Bos taurus replication timing regulatory factor 1 (RIF1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OBa55068	XM_010826493.2 Latest version!	Bos taurus replication timing regulatory factor 1 (RIF1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OBa55068	XM_010802077.3 Latest version!	Bos taurus replication timing regulatory factor 1 (RIF1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
View Old Accession Versions >>
OBa55065	XM_010802073.2	Bos taurus replication timing regulatory factor 1 (RIF1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OBa55068	XM_010802077.2	Bos taurus replication timing regulatory factor 1 (RIF1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OBa02875	XM_002685359.5	Bos taurus replication timing regulatory factor 1 (RIF1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OBa55067	XM_010802075.2	Bos taurus replication timing regulatory factor 1 (RIF1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OBa55066	XM_010802074.2	Bos taurus replication timing regulatory factor 1 (RIF1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
Hide Old Accession Versions >>

RIF1 ( XM_010826492.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010824794.1 Bos taurus replication timing regulatory factor 1 (RIF1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OBa02875
Clone ID Related Accession (Same CDS sequence)	XM_010826492.2 , XM_002685359.6 , XM_002685359.5 , XM_024980692.1 , XM_024980695.1 , XM_010802076.2 , XM_615269.9
Accession Version	XM_010826492.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7398bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	telomere-associated protein RIF1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644591.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010826492.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGCCG CGGATCAGAG CCCCCTGGCA CCGCTGTTGG AGACTTTGGA AGACCCTTCC 
GCCTCCCTTG GAGAGCAGAC CGACGCTTAC CTGACCCTGA CCAGTCGTAT GACTGGAGAA 
GATGGAAAAG AAATCATTAC AGAAATTGAG AAAAAACTTC CTCAGCTATT TAAAGTTTTA 
AAGACTCACA TTGCCAGTCA AAACTCAGAG CTCAGTAGTG CTGCTCTACA GGCCTTGGGG 
TTTTGCTTAT ATAATCCCAA AATTGCCTCT GGATTATCAG AGATAAATAT TCAAGAACTG 
CTTTCAAAAC TGAATGATAT TGTTAAAAGT TCAGATAAAA ATGTACGTAC TAGGGCACTT 
TGGGTGATAT CCAAGCAGAC GTTTCCCACT GAAGTCGTTG GCAAAGTAGT TTCTAGTATC 
ATTGACTCAC TAGAAATAGT ATTTAATAGA GGAGAGATGC TTTCTGCTGT TGTGGATTAT 
GAAGCATTAA ATGTCATTAT AAGACTAATT GAACAAGCCC CAGTTCAAAT GGGAGAAGAG 
TCAATTAGGT GGGCAAAATT GGTCATACCT TTACTGGTTC ATTCAGCCCA AAAAGTACAT 
TTGCGGGGAG CCACTGCTCT AGAGATGGGA ATGCCTTTGT TGCTTCAGAA ACAGCAAGAA 
ATAGCATCTA TCACAGAGCA GCTTATGACT ACCAAATTAC TTTCAGAACT CCAGAAACTG 
TTTATGAGTA AAAATGAGAC TTATGTGTTA AAATTGTGGC CTTTATTTGT CAAACTTCTT 
GGGAAGACCT TGCATCGAAG TGGGAGTTTC ATTAATTCTC TGTTACAGCT GGAAGAACTG 
GGATTTCGTA GTGGAGCACC CATGATTAAA AAAATAGCTT TTATTGCTTG GAAGAGTTTA 
ATAGATAATT TTGCTTTAAA TCCAGAAATA CTGTGTAGTG CAAAAAGACT GAAGTTGTTG 
ATGCAGCCTT TGAGTTCTAT CCATGTGAGA ACAGAAACTC TAGCGCTGAC AAAACTAGAA 
GTCTGGTGGT ATTTGTTGAT GAGACTAGGA CCTCATCTTC CTGCTAATTT TGAACAGGTT 
TGTGTGCCTC TGATTCAGAG CACAATAAGC ATTGATTCTA ATGCTTCACC TCAAGGGAGT 
TCATCTCGTG TAGCTACTAC TCCAGGTTTA AGCCCTATGA CCCCAGTACA CAAAGGTGCT 
TCTCCATATG GAACTCCTGT AACCCCTCGG ATGAACTTGA ATTCAAATTT TGGAATGGCC 
ACAATCCCCT CTATACAACT TTTGGGGCTT GAAATGTTGC TTCATTTTTT GTTGGGTCCA 
GAAGTCGTGA GTTTTGCTAA GCAAAACAAA CTTATACTGA GCCTAGAACC ACTTGAACAT 
CCATTAATCA GCAGCTCTTC TTTTTTCTCC AAACACTCAA ATACACTTAT CACTGCTGTT 
CATGATAGCT TTGTTGCAGT TGGAAAAGAT GCTTCTGATG TAGTTATTAA TGCTATTTGG 
AAGGAGTTAA TTAGCTTGGT GAAGTCAGTT ATTGAATCAG GTAACAAAAA AGAGAGACCA 
GGTTCTGAAG TTTTGACTCT CTTATTAAAA TCTTTGGAAA GCATAGTGAA TTCAGAAGTA 
TTTCCTGTAT TGAAAACACT GGTTCTCATG GAAATTACAA TTAAAGGACT TCCTCAGAAA 
GTATTAGGTT CTCCAGCATA TCAGGTTGCT AATATGGATA TTCTTAATGG AACCCCTGCT 
TTGTTCTTAA TTCAGTTAAC TTTCAATAAT AATCTATTGG AATGTGGTGT GGAAGACGAA 
AGGTTTTTTC TCAACCTGGA AACACTTGTG GGCTGTGTTC TTTCGGGTCC AACTTCACCA 
CTCGCTTTCA GTGACTCTGT CTTAAATGTT GTCAATCAAA ATGCAAAGCA GTTGGATAAC 
AAGGAGCATC TTTGGAGAAT GTGGAGTATT ATAGTCACCC CATTAACAGA ATTGATTAAT 
CAGACCAATG AAGTGAATCA AGGGGATGCC TTAGAACATA ATTTCAGTGC TATCTATGGT 
GCACTGATTT TGCCAATAAA CCATATTTTT CCAGCACAAA AAATTCCAGT GGTCACCATG 
AAGACCTTAC TTAAAACTTG GTCAGAATTA TATAGAGCAT TTGCTCGCTG TGCAGCTTTG 
GTGGCAACTG CGGAAGAGAA TTTATGCTGT GAGGAATTTT GTTCCAAGAT AATGTCCAGT 
TTGGAAGATG AAGGCCTTTC AAATTTATTG TTCTTGGATA GGATCACTCA TATTATTACT 
GTAATGGTTG ATTGCATCGA CTTTTCACCA TATAATATTA AATATCAACC CAAAACTAAA 
TCACCACAGA GACCTACAGA TTGGTCCAAA AAGAAGAAGG AACCTTTAGG GAAGTTGGCT 
TCTTTAATTA AACTTATTGT GAGAGTGATC TATTCTTTTC ACACACTGAG CCTCAAGGAA 
GTACATTCTG ATACTCTCCT CACTATTGGC AACTCAATCA CCAGCATTCT TTCCAATTTA 
CTTGGACATA TTTCTCTGCC TTCTATGATC CAGAAAATAT TTGCAGCTTT AACAAGACCT 
CTGGCATTAT TTTATGAAAA CTCAAAGCTT GATGAAGTTC CTAAAGTGTA TAGTTGTATG 
AACAACAAGT TAGAAAAGCT ACTAGGAGAT ATAATTGCTT GTCTACATGT CAGCTACACT 
GGAACTTACG ACAGTGAACT TCTTGAACAG ATCTCACCAT TATTATGCAT AATATTTTCA 
CACAAGAACA AACAGATAAG AAAACAAAGT GCTCAGTTCT GGAATGCCAC ATTTGCTAAA 
GTGACGATGT TGATTTATCC TGAGGAGTTA AAGCCAATAC TGAAACAAGC CAAACAAAAA 
TCTCTGCTTT TATTGCCTGG TTTGGAAAAT GTTGAAATTA TGGAGGAATC CAGTGGACCA 
TATTCAGATA TGACAGAAAA TTCCCAATTA AATATGAAGA TAAGTGGCAT GGAAAGAAAA 
TCAAGTGGAA AAAGAGATTC TTTTTTGGCA CAAACAAAGG ATACAAAAGA CAGTATGAAA 
CCACCAGCTA AATTGAAACT AGATTCTTCA ACTCCAAAAG CAAAGGGTGA AATGCCTTTG 
GAAGAAGAAA AGTCTACTGA CTTTGTGTTT ATACCTCCAG AAGTAAAAGA AACAAAGGAA 
AGAATACTAA CTGAACACCA GAAAGAAGTA CTCAAAACAA AGAGGTGTGA TATTCCTGCC 
ATGTATAATA ATCTGGATGT TTCACAAGAT ACTTTATTTT CTCAGTATAG TCAGGAAGAA 
TCTATGGAAA TTTCTACTTT AATTGAAAAA TCAAAAGAAG ACTCCAAGAT GATATTTAAG 
GAGGAACAAA AGGAGAATGA CATTGTCGTT CCTCAACATG TCATGGAAAA CTGCGGTATG 
GATGAACATC TTGAAAAGGC TTCCCTTTTG AATAATGAGT GTGGTTCTAT TGAGGAAACC 
AGTCCAAAAA TACTGAGCAG TAATAATGAT GCCAGAAAAA AAGCTTTAAT TGCTTCAAAT 
AAACCAACAG AATGTGCATC TACTACAGAA AATCCATCTG GTGTCAGCAG TAGCTCGGTT 
TCTATTGCCA CCATTTCTGG AGCTCCCTTA CAGCCTACAA GTCGAAGGCA ATCCTTTATT 
ACTTTGGAAA AGTTTGATGG TTCAGAAAAT AGACCTTTTA GTCCATCACC CTTGAATAAT 
ATGTCATCAA CTGTAATAGT GAAAAGTAAC CAGGAAGGCA TGGGTAAAAC AGATAATCCA 
TCAAAAGCAA AGAAGAGAGA AGTGGCTCTT TCCAAATCTG ACTCTGAAAA AATAACACAT 
GGGACTAAGA GATCAAGCCG AAGGTCGAGT AAATCTGAAC CAATAGGGAG TAAAAAGTCT 
AAACCCTTAA TGAGATCTGA ACAAGAGAAA AGTACTCAAG AATCTGTTGA TGGCACAGTA 
GCCTTAGAAA ATAACCCACC TGGCTTGCTT AATCAGACAG AATGTGTGTT AGATAATCAG 
GTTCATCTCT CTGAATCTAC AGTGGAGTTT GAGGATACAA TGCTTGAATC AACAGTGGAG 
AATACTGTAT TAGAAAACAA TACTGTAGAA GAGAGAACCT TAGAAGTTAA CTTGGAATCC 
AAAGAAAACA CACCCCCAGC CATAATAACG GATCAGACAG TAAATGAGGA TAGTCATATT 
CAGGTAACTC CAAATCAGAA AACCCTTAGG CGATCTTTAA GGCGACGTTC AGAAACAGCA 
GAACCAACCA CTGATAGCCA AGATAAAGAA AATAATCATC AAAAAAGGGA AAGACGTAAA 
GAAGAAGAAA AGACTCCGCA GAAGAGTCCG TTACATGTAA AAGATGATGT TTTACCTAAG 
CAAAAATTGA TTTCTGAACA AAGTGTACAG GGAAACTTAG TTGACAAAGG AAATAATTTA 
CATGAGAAGG CTTTTGGGGA GACCAGTGCT AATGCTGAAA TTGATGAAAA TAGAAGAAAG 
CCAGATCTTG AGAATACTAA ATCTGAAGGA GATGGTGTGC CGGACATTGC AGATAAATCC 
TCTGAGAAAC CAGTAAGGGG ACGAACACGA TACCAAACAA GAAGAGCATC CCAGGGTTTA 
CTTTCCAGCA TTGAAAACTC AGAATCTGAT AGTTCTGAGG CAAAAGAAGA AGGTTCTAAA 
AAGAAAAGAT CTGGAAAATG GAAGAACAAA AGCAGTGACA ATGTTGACAT GGAAGATCAA 
GAAGAGAAAA TGGTAAAACA GGAATGTATA AAAATTGAAA ATCAGGCACA TGATTATAAA 
GGGAATCCTG AAGTAGACAA AGATATAAAA TCTCAAATCT GTGAAAAAGG AGATGAAAGT 
ACTACTGTAA GTCTTCAAGA TTCTACCGTA TCTCCAGATT TATTACAAGT TTATGATGAT 
GTATTGAATA CTTCTGAGGG AGAAAGTAAA AGTAACAAAG ATGCAGACCA TTCCTTTTCA 
AATCCTTCTG TACCAGAATC AAATCTAAGG ACTAGAAATG CCAGTAAGAG GTTACATAAG 
CGAGATTCTA TAGAAAATAG CATGGGGGAA TCCTCAAAAA TAGGGACATC AGACATTTCT 
TTGCTTTCTG AAAAAACTCT TCAGACAGTA GAATGCCAGC ACAAGAGAAG TAGGAGGGTG 
AGGCGATCTA AAGGTTGTGA TTGTTGTGGA GAAAAATCAC AGTCTCAGGA AAAGTCATTT 
ACTGGGTTAA AGGATACAGA AAATTACGAT GTAAAAGTCA GTGAAACAAA AAAGACAGAT 
GGGGAAACAC CTGTATCTAT TTCAGAAACT TCGAAAGCTG ATCTGTCCTC GGAGGTTAAA 
CTTTTAGATG AGCATCATAG TGTGAATTTT CATTTGGATC TCAAGGAAGA GAATGATACT 
ACTAATGATT CATTAATTAT ATCTGAAACT GAGTTAAAAG AAAATACTAC TCAAAGCTTT 
CTTCCTTCTG AAATGGTAAA ATTTAAAGAA ACTTCTCGTA GGGATTCTGA GGAAGCAATA 
TCTCTAGAAA CCCAGGAGCC ATCTAATAAG AAATGTAAAA CTGTTGACCC ACATTTAGAT 
GTTTCCAAAG ATATTTCACT GAAATGTTTT TCTTTGGAGA CTAAAGAAGA AAAGTCAGAG 
GCCACTTCAA AAGAGAAATT GAGTACAGAG AACATTGTTA TTGTTGAATC AAATGCAGAA 
TCAAATCCAG AAGTAGATGC TATGGAATTG AATGTAGAAA ATGATCAGTC TGAAGCTGGC 
TTCTCTGAAC AAAAGAATGT CAAAACTGGT ATTCCAGGGA AAGAAGTAAC AGAGGAAAAC 
AGTGAAAGAA TGGATACACC TGAAAAACTG AAAGCCAATG AAGCAGTAAC TGAGGAATTT 
AATTCAGCTA CTGATTATTC GGATACTCCG ACATCTGTAA AAGTGAATGC TCAGGTAGAG 
ATCTCTGAAC AAATAGCGGT TGGGGAGCTA GGTGGAGGAA GTGATGAATC TGATCCAGGC 
TCATCTGAAG AAATGAACGC TGAAATGAAA AATTGTGAAA AAACAGCGGT TTGTGAGGTG 
ACTACTGAAT CTGACCACGT CACTGAAACC GAAGACGAAG ATGTAACTAC CACGGCTTCT 
CTGCCTATAG AAGTTGAAAC AAAGGAATTA GATGTAGAAG TCAATAACTT TGTTCCCGAC 
ACACTTGAGA TGATCACTGA AGAAGGAAAG GTTGACCCTA ACAAAACTGA AACGAATACT 
GAACCTAATA AATTGGAGGA AACACAGTTA GATGACAATC AGATGGTAGT GGAAAGTAAT 
GAAACTTTAC CGGAAGTTCA CCATACTTCA GAGAAAGTAG AGGAGATAGC ACAACCTCTG 
ACATCTGAAA CAGATATTTC TGGACTAATA TCAGAAGACA GTAATACATC TCCTCAAAAA 
TCGAAGGATA TTGATCCTTT ACTCATGTCA GCAAACGAGA GTCCTAGTGG CATGCACACA 
CGTTGTGTGT GGTCTCCTTT GGCTTCTCCA TCCACCAGCA TTTTAAAGAG AGGACTAAAA 
AGACCCCAAG AAGATGAAAT CTCGTCACCT GTCCACAAGG TTCGCCGTGT CTCCTTTGCA 
GATCCTATAT ACCAAGCAGG ATTGGCAGAT GACATTGATA GGCGATGCTC TATTGTTAGG 
TGCCATTCTT CAAATAGTTC TCTCATGGTA AAAAGTGTTA AAACTTCACC TACTGTGCAA 
TCTAAGCATA ATACCACTTC AGCCAAAGGA TTTCTGTCCC CAGGATCACG TAGCTCTAAA 
TTTAAGAGCT CAAAGAAGTG TTTAATTGCA GAAATGGCTA AGGAATCTGT GCCATGCCCA 
ACAGAAAGTG TTTACCCTGC TTTGGTGAAC TGTGTAGCAC CAGTTGAAAT CATTTTACCT 
CAGATTACAT CCAACATGTG GGCAAGAGGA CTGGGACAGC TCATTAGAGC CAAGAATATA 
AAAACAATTG GCGATTTGAG TACCCTTACA GCATCTGAAA TAAAAACTCT TCCTATCCGT 
TCTCCAAAAG TGTCTAACGT TAAAAAGGCT CTCAGAATAT ATCATGAGCA GCAGGTGAAG 
TCTCGTGGAC TAGAAGAGAT TCCAGTTTTT GATATTTCTG AAAAAACAAT AAATGGAATG 
GAAAATAAAT CTATCTCTCC TGATGAAGAA AGACTTGCCT CAGATTTAAT TGATCCTGTT 
GCTTTAGAAG CTCCGTTATC TAAAAATCTT GTGGCACAGA TTAGCGCACT TGCTCTTCAA 
CTAGATTCAG AAGATCTCTA TAATTATTCA GGAAGCCAGC TATTTGAAAT GCACGAGAAA 
CTTGGTAGCA TGGCAAACTC TATAATTAAA AATCTGCAGT CACGTTGGAG GTCACCAGCC 
CATGAAAATT CTTTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010824794.1
CDS	288..7685
Translation	MTAADQSPLAPLLETLEDPSASLGEQTDAYLTLTSRMTGEDGKEIITEIEKKLPQLFKVLKTHIASQNSELSSAALQALGFCLYNPKIASGLSEINIQELLSKLNDIVKSSDKNVRTRALWVISKQTFPTEVVGKVVSSIIDSLEIVFNRGEMLSAVVDYEALNVIIRLIEQAPVQMGEESIRWAKLVIPLLVHSAQKVHLRGATALEMGMPLLLQKQQEIASITEQLMTTKLLSELQKLFMSKNETYVLKLWPLFVKLLGKTLHRSGSFINSLLQLEELGFRSGAPMIKKIAFIAWKSLIDNFALNPEILCSAKRLKLLMQPLSSIHVRTETLALTKLEVWWYLLMRLGPHLPANFEQVCVPLIQSTISIDSNASPQGSSSRVATTPGLSPMTPVHKGASPYGTPVTPRMNLNSNFGMATIPSIQLLGLEMLLHFLLGPEVVSFAKQNKLILSLEPLEHPLISSSSFFSKHSNTLITAVHDSFVAVGKDASDVVINAIWKELISLVKSVIESGNKKERPGSEVLTLLLKSLESIVNSEVFPVLKTLVLMEITIKGLPQKVLGSPAYQVANMDILNGTPALFLIQLTFNNNLLECGVEDERFFLNLETLVGCVLSGPTSPLAFSDSVLNVVNQNAKQLDNKEHLWRMWSIIVTPLTELINQTNEVNQGDALEHNFSAIYGALILPINHIFPAQKIPVVTMKTLLKTWSELYRAFARCAALVATAEENLCCEEFCSKIMSSLEDEGLSNLLFLDRITHIITVMVDCIDFSPYNIKYQPKTKSPQRPTDWSKKKKEPLGKLASLIKLIVRVIYSFHTLSLKEVHSDTLLTIGNSITSILSNLLGHISLPSMIQKIFAALTRPLALFYENSKLDEVPKVYSCMNNKLEKLLGDIIACLHVSYTGTYDSELLEQISPLLCIIFSHKNKQIRKQSAQFWNATFAKVTMLIYPEELKPILKQAKQKSLLLLPGLENVEIMEESSGPYSDMTENSQLNMKISGMERKSSGKRDSFLAQTKDTKDSMKPPAKLKLDSSTPKAKGEMPLEEEKSTDFVFIPPEVKETKERILTEHQKEVLKTKRCDIPAMYNNLDVSQDTLFSQYSQEESMEISTLIEKSKEDSKMIFKEEQKENDIVVPQHVMENCGMDEHLEKASLLNNECGSIEETSPKILSSNNDARKKALIASNKPTECASTTENPSGVSSSSVSIATISGAPLQPTSRRQSFITLEKFDGSENRPFSPSPLNNMSSTVIVKSNQEGMGKTDNPSKAKKREVALSKSDSEKITHGTKRSSRRSSKSEPIGSKKSKPLMRSEQEKSTQESVDGTVALENNPPGLLNQTECVLDNQVHLSESTVEFEDTMLESTVENTVLENNTVEERTLEVNLESKENTPPAIITDQTVNEDSHIQVTPNQKTLRRSLRRRSETAEPTTDSQDKENNHQKRERRKEEEKTPQKSPLHVKDDVLPKQKLISEQSVQGNLVDKGNNLHEKAFGETSANAEIDENRRKPDLENTKSEGDGVPDIADKSSEKPVRGRTRYQTRRASQGLLSSIENSESDSSEAKEEGSKKKRSGKWKNKSSDNVDMEDQEEKMVKQECIKIENQAHDYKGNPEVDKDIKSQICEKGDESTTVSLQDSTVSPDLLQVYDDVLNTSEGESKSNKDADHSFSNPSVPESNLRTRNASKRLHKRDSIENSMGESSKIGTSDISLLSEKTLQTVECQHKRSRRVRRSKGCDCCGEKSQSQEKSFTGLKDTENYDVKVSETKKTDGETPVSISETSKADLSSEVKLLDEHHSVNFHLDLKEENDTTNDSLIISETELKENTTQSFLPSEMVKFKETSRRDSEEAISLETQEPSNKKCKTVDPHLDVSKDISLKCFSLETKEEKSEATSKEKLSTENIVIVESNAESNPEVDAMELNVENDQSEAGFSEQKNVKTGIPGKEVTEENSERMDTPEKLKANEAVTEEFNSATDYSDTPTSVKVNAQVEISEQIAVGELGGGSDESDPGSSEEMNAEMKNCEKTAVCEVTTESDHVTETEDEDVTTTASLPIEVETKELDVEVNNFVPDTLEMITEEGKVDPNKTETNTEPNKLEETQLDDNQMVVESNETLPEVHHTSEKVEEIAQPLTSETDISGLISEDSNTSPQKSKDIDPLLMSANESPSGMHTRCVWSPLASPSTSILKRGLKRPQEDEISSPVHKVRRVSFADPIYQAGLADDIDRRCSIVRCHSSNSSLMVKSVKTSPTVQSKHNTTSAKGFLSPGSRSSKFKSSKKCLIAEMAKESVPCPTESVYPALVNCVAPVEIILPQITSNMWARGLGQLIRAKNIKTIGDLSTLTASEIKTLPIRSPKVSNVKKALRIYHEQQVKSRGLEEIPVFDISEKTINGMENKSISPDEERLASDLIDPVALEAPLSKNLVAQISALALQLDSEDLYNYSGSQLFEMHEKLGSMANSIIKNLQSRWRSPAHENSF

Target ORF information:

RefSeq Version	XM_010826492.2
Organism	Bos taurus(cattle)
Definition	Bos taurus replication timing regulatory factor 1 (RIF1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010826492.2

ORF Insert Sequence:

ATGACGGCCG CGGATCAGAG CCCCCTGGCA CCGCTGTTGG AGACTTTGGA AGACCCTTCC 
GCCTCCCTTG GAGAGCAGAC CGACGCTTAC CTGACCCTGA CCAGTCGTAT GACTGGAGAA 
GATGGAAAAG AAATCATTAC AGAAATTGAG AAAAAACTTC CTCAGCTATT TAAAGTTTTA 
AAGACTCACA TTGCCAGTCA AAACTCAGAG CTCAGTAGTG CTGCTCTACA GGCCTTGGGG 
TTTTGCTTAT ATAATCCCAA AATTGCCTCT GGATTATCAG AGATAAATAT TCAAGAACTG 
CTTTCAAAAC TGAATGATAT TGTTAAAAGT TCAGATAAAA ATGTACGTAC TAGGGCACTT 
TGGGTGATAT CCAAGCAGAC GTTTCCCACT GAAGTCGTTG GCAAAGTAGT TTCTAGTATC 
ATTGACTCAC TAGAAATAGT ATTTAATAGA GGAGAGATGC TTTCTGCTGT TGTGGATTAT 
GAAGCATTAA ATGTCATTAT AAGACTAATT GAACAAGCCC CAGTTCAAAT GGGAGAAGAG 
TCAATTAGGT GGGCAAAATT GGTCATACCT TTACTGGTTC ATTCAGCCCA AAAAGTACAT 
TTGCGGGGAG CCACTGCTCT AGAGATGGGA ATGCCTTTGT TGCTTCAGAA ACAGCAAGAA 
ATAGCATCTA TCACAGAGCA GCTTATGACT ACCAAATTAC TTTCAGAACT CCAGAAACTG 
TTTATGAGTA AAAATGAGAC TTATGTGTTA AAATTGTGGC CTTTATTTGT CAAACTTCTT 
GGGAAGACCT TGCATCGAAG TGGGAGTTTC ATTAATTCTC TGTTACAGCT GGAAGAACTG 
GGATTTCGTA GTGGAGCACC CATGATTAAA AAAATAGCTT TTATTGCTTG GAAGAGTTTA 
ATAGATAATT TTGCTTTAAA TCCAGAAATA CTGTGTAGTG CAAAAAGACT GAAGTTGTTG 
ATGCAGCCTT TGAGTTCTAT CCATGTGAGA ACAGAAACTC TAGCGCTGAC AAAACTAGAA 
GTCTGGTGGT ATTTGTTGAT GAGACTAGGA CCTCATCTTC CTGCTAATTT TGAACAGGTT 
TGTGTGCCTC TGATTCAGAG CACAATAAGC ATTGATTCTA ATGCTTCACC TCAAGGGAGT 
TCATCTCGTG TAGCTACTAC TCCAGGTTTA AGCCCTATGA CCCCAGTACA CAAAGGTGCT 
TCTCCATATG GAACTCCTGT AACCCCTCGG ATGAACTTGA ATTCAAATTT TGGAATGGCC 
ACAATCCCCT CTATACAACT TTTGGGGCTT GAAATGTTGC TTCATTTTTT GTTGGGTCCA 
GAAGTCGTGA GTTTTGCTAA GCAAAACAAA CTTATACTGA GCCTAGAACC ACTTGAACAT 
CCATTAATCA GCAGCTCTTC TTTTTTCTCC AAACACTCAA ATACACTTAT CACTGCTGTT 
CATGATAGCT TTGTTGCAGT TGGAAAAGAT GCTTCTGATG TAGTTATTAA TGCTATTTGG 
AAGGAGTTAA TTAGCTTGGT GAAGTCAGTT ATTGAATCAG GTAACAAAAA AGAGAGACCA 
GGTTCTGAAG TTTTGACTCT CTTATTAAAA TCTTTGGAAA GCATAGTGAA TTCAGAAGTA 
TTTCCTGTAT TGAAAACACT GGTTCTCATG GAAATTACAA TTAAAGGACT TCCTCAGAAA 
GTATTAGGTT CTCCAGCATA TCAGGTTGCT AATATGGATA TTCTTAATGG AACCCCTGCT 
TTGTTCTTAA TTCAGTTAAC TTTCAATAAT AATCTATTGG AATGTGGTGT GGAAGACGAA 
AGGTTTTTTC TCAACCTGGA AACACTTGTG GGCTGTGTTC TTTCGGGTCC AACTTCACCA 
CTCGCTTTCA GTGACTCTGT CTTAAATGTT GTCAATCAAA ATGCAAAGCA GTTGGATAAC 
AAGGAGCATC TTTGGAGAAT GTGGAGTATT ATAGTCACCC CATTAACAGA ATTGATTAAT 
CAGACCAATG AAGTGAATCA AGGGGATGCC TTAGAACATA ATTTCAGTGC TATCTATGGT 
GCACTGATTT TGCCAATAAA CCATATTTTT CCAGCACAAA AAATTCCAGT GGTCACCATG 
AAGACCTTAC TTAAAACTTG GTCAGAATTA TATAGAGCAT TTGCTCGCTG TGCAGCTTTG 
GTGGCAACTG CGGAAGAGAA TTTATGCTGT GAGGAATTTT GTTCCAAGAT AATGTCCAGT 
TTGGAAGATG AAGGCCTTTC AAATTTATTG TTCTTGGATA GGATCACTCA TATTATTACT 
GTAATGGTTG ATTGCATCGA CTTTTCACCA TATAATATTA AATATCAACC CAAAACTAAA 
TCACCACAGA GACCTACAGA TTGGTCCAAA AAGAAGAAGG AACCTTTAGG GAAGTTGGCT 
TCTTTAATTA AACTTATTGT GAGAGTGATC TATTCTTTTC ACACACTGAG CCTCAAGGAA 
GTACATTCTG ATACTCTCCT CACTATTGGC AACTCAATCA CCAGCATTCT TTCCAATTTA 
CTTGGACATA TTTCTCTGCC TTCTATGATC CAGAAAATAT TTGCAGCTTT AACAAGACCT 
CTGGCATTAT TTTATGAAAA CTCAAAGCTT GATGAAGTTC CTAAAGTGTA TAGTTGTATG 
AACAACAAGT TAGAAAAGCT ACTAGGAGAT ATAATTGCTT GTCTACATGT CAGCTACACT 
GGAACTTACG ACAGTGAACT TCTTGAACAG ATCTCACCAT TATTATGCAT AATATTTTCA 
CACAAGAACA AACAGATAAG AAAACAAAGT GCTCAGTTCT GGAATGCCAC ATTTGCTAAA 
GTGACGATGT TGATTTATCC TGAGGAGTTA AAGCCAATAC TGAAACAAGC CAAACAAAAA 
TCTCTGCTTT TATTGCCTGG TTTGGAAAAT GTTGAAATTA TGGAGGAATC CAGTGGACCA 
TATTCAGATA TGACAGAAAA TTCCCAATTA AATATGAAGA TAAGTGGCAT GGAAAGAAAA 
TCAAGTGGAA AAAGAGATTC TTTTTTGGCA CAAACAAAGG ATACAAAAGA CAGTATGAAA 
CCACCAGCTA AATTGAAACT AGATTCTTCA ACTCCAAAAG CAAAGGGTGA AATGCCTTTG 
GAAGAAGAAA AGTCTACTGA CTTTGTGTTT ATACCTCCAG AAGTAAAAGA AACAAAGGAA 
AGAATACTAA CTGAACACCA GAAAGAAGTA CTCAAAACAA AGAGGTGTGA TATTCCTGCC 
ATGTATAATA ATCTGGATGT TTCACAAGAT ACTTTATTTT CTCAGTATAG TCAGGAAGAA 
TCTATGGAAA TTTCTACTTT AATTGAAAAA TCAAAAGAAG ACTCCAAGAT GATATTTAAG 
GAGGAACAAA AGGAGAATGA CATTGTCGTT CCTCAACATG TCATGGAAAA CTGCGGTATG 
GATGAACATC TTGAAAAGGC TTCCCTTTTG AATAATGAGT GTGGTTCTAT TGAGGAAACC 
AGTCCAAAAA TACTGAGCAG TAATAATGAT GCCAGAAAAA AAGCTTTAAT TGCTTCAAAT 
AAACCAACAG AATGTGCATC TACTACAGAA AATCCATCTG GTGTCAGCAG TAGCTCGGTT 
TCTATTGCCA CCATTTCTGG AGCTCCCTTA CAGCCTACAA GTCGAAGGCA ATCCTTTATT 
ACTTTGGAAA AGTTTGATGG TTCAGAAAAT AGACCTTTTA GTCCATCACC CTTGAATAAT 
ATGTCATCAA CTGTAATAGT GAAAAGTAAC CAGGAAGGCA TGGGTAAAAC AGATAATCCA 
TCAAAAGCAA AGAAGAGAGA AGTGGCTCTT TCCAAATCTG ACTCTGAAAA AATAACACAT 
GGGACTAAGA GATCAAGCCG AAGGTCGAGT AAATCTGAAC CAATAGGGAG TAAAAAGTCT 
AAACCCTTAA TGAGATCTGA ACAAGAGAAA AGTACTCAAG AATCTGTTGA TGGCACAGTA 
GCCTTAGAAA ATAACCCACC TGGCTTGCTT AATCAGACAG AATGTGTGTT AGATAATCAG 
GTTCATCTCT CTGAATCTAC AGTGGAGTTT GAGGATACAA TGCTTGAATC AACAGTGGAG 
AATACTGTAT TAGAAAACAA TACTGTAGAA GAGAGAACCT TAGAAGTTAA CTTGGAATCC 
AAAGAAAACA CACCCCCAGC CATAATAACG GATCAGACAG TAAATGAGGA TAGTCATATT 
CAGGTAACTC CAAATCAGAA AACCCTTAGG CGATCTTTAA GGCGACGTTC AGAAACAGCA 
GAACCAACCA CTGATAGCCA AGATAAAGAA AATAATCATC AAAAAAGGGA AAGACGTAAA 
GAAGAAGAAA AGACTCCGCA GAAGAGTCCG TTACATGTAA AAGATGATGT TTTACCTAAG 
CAAAAATTGA TTTCTGAACA AAGTGTACAG GGAAACTTAG TTGACAAAGG AAATAATTTA 
CATGAGAAGG CTTTTGGGGA GACCAGTGCT AATGCTGAAA TTGATGAAAA TAGAAGAAAG 
CCAGATCTTG AGAATACTAA ATCTGAAGGA GATGGTGTGC CGGACATTGC AGATAAATCC 
TCTGAGAAAC CAGTAAGGGG ACGAACACGA TACCAAACAA GAAGAGCATC CCAGGGTTTA 
CTTTCCAGCA TTGAAAACTC AGAATCTGAT AGTTCTGAGG CAAAAGAAGA AGGTTCTAAA 
AAGAAAAGAT CTGGAAAATG GAAGAACAAA AGCAGTGACA ATGTTGACAT GGAAGATCAA 
GAAGAGAAAA TGGTAAAACA GGAATGTATA AAAATTGAAA ATCAGGCACA TGATTATAAA 
GGGAATCCTG AAGTAGACAA AGATATAAAA TCTCAAATCT GTGAAAAAGG AGATGAAAGT 
ACTACTGTAA GTCTTCAAGA TTCTACCGTA TCTCCAGATT TATTACAAGT TTATGATGAT 
GTATTGAATA CTTCTGAGGG AGAAAGTAAA AGTAACAAAG ATGCAGACCA TTCCTTTTCA 
AATCCTTCTG TACCAGAATC AAATCTAAGG ACTAGAAATG CCAGTAAGAG GTTACATAAG 
CGAGATTCTA TAGAAAATAG CATGGGGGAA TCCTCAAAAA TAGGGACATC AGACATTTCT 
TTGCTTTCTG AAAAAACTCT TCAGACAGTA GAATGCCAGC ACAAGAGAAG TAGGAGGGTG 
AGGCGATCTA AAGGTTGTGA TTGTTGTGGA GAAAAATCAC AGTCTCAGGA AAAGTCATTT 
ACTGGGTTAA AGGATACAGA AAATTACGAT GTAAAAGTCA GTGAAACAAA AAAGACAGAT 
GGGGAAACAC CTGTATCTAT TTCAGAAACT TCGAAAGCTG ATCTGTCCTC GGAGGTTAAA 
CTTTTAGATG AGCATCATAG TGTGAATTTT CATTTGGATC TCAAGGAAGA GAATGATACT 
ACTAATGATT CATTAATTAT ATCTGAAACT GAGTTAAAAG AAAATACTAC TCAAAGCTTT 
CTTCCTTCTG AAATGGTAAA ATTTAAAGAA ACTTCTCGTA GGGATTCTGA GGAAGCAATA 
TCTCTAGAAA CCCAGGAGCC ATCTAATAAG AAATGTAAAA CTGTTGACCC ACATTTAGAT 
GTTTCCAAAG ATATTTCACT GAAATGTTTT TCTTTGGAGA CTAAAGAAGA AAAGTCAGAG 
GCCACTTCAA AAGAGAAATT GAGTACAGAG AACATTGTTA TTGTTGAATC AAATGCAGAA 
TCAAATCCAG AAGTAGATGC TATGGAATTG AATGTAGAAA ATGATCAGTC TGAAGCTGGC 
TTCTCTGAAC AAAAGAATGT CAAAACTGGT ATTCCAGGGA AAGAAGTAAC AGAGGAAAAC 
AGTGAAAGAA TGGATACACC TGAAAAACTG AAAGCCAATG AAGCAGTAAC TGAGGAATTT 
AATTCAGCTA CTGATTATTC GGATACTCCG ACATCTGTAA AAGTGAATGC TCAGGTAGAG 
ATCTCTGAAC AAATAGCGGT TGGGGAGCTA GGTGGAGGAA GTGATGAATC TGATCCAGGC 
TCATCTGAAG AAATGAACGC TGAAATGAAA AATTGTGAAA AAACAGCGGT TTGTGAGGTG 
ACTACTGAAT CTGACCACGT CACTGAAACC GAAGACGAAG ATGTAACTAC CACGGCTTCT 
CTGCCTATAG AAGTTGAAAC AAAGGAATTA GATGTAGAAG TCAATAACTT TGTTCCCGAC 
ACACTTGAGA TGATCACTGA AGAAGGAAAG GTTGACCCTA ACAAAACTGA AACGAATACT 
GAACCTAATA AATTGGAGGA AACACAGTTA GATGACAATC AGATGGTAGT GGAAAGTAAT 
GAAACTTTAC CGGAAGTTCA CCATACTTCA GAGAAAGTAG AGGAGATAGC ACAACCTCTG 
ACATCTGAAA CAGATATTTC TGGACTAATA TCAGAAGACA GTAATACATC TCCTCAAAAA 
TCGAAGGATA TTGATCCTTT ACTCATGTCA GCAAACGAGA GTCCTAGTGG CATGCACACA 
CGTTGTGTGT GGTCTCCTTT GGCTTCTCCA TCCACCAGCA TTTTAAAGAG AGGACTAAAA 
AGACCCCAAG AAGATGAAAT CTCGTCACCT GTCCACAAGG TTCGCCGTGT CTCCTTTGCA 
GATCCTATAT ACCAAGCAGG ATTGGCAGAT GACATTGATA GGCGATGCTC TATTGTTAGG 
TGCCATTCTT CAAATAGTTC TCTCATGGTA AAAAGTGTTA AAACTTCACC TACTGTGCAA 
TCTAAGCATA ATACCACTTC AGCCAAAGGA TTTCTGTCCC CAGGATCACG TAGCTCTAAA 
TTTAAGAGCT CAAAGAAGTG TTTAATTGCA GAAATGGCTA AGGAATCTGT GCCATGCCCA 
ACAGAAAGTG TTTACCCTGC TTTGGTGAAC TGTGTAGCAC CAGTTGAAAT CATTTTACCT 
CAGATTACAT CCAACATGTG GGCAAGAGGA CTGGGACAGC TCATTAGAGC CAAGAATATA 
AAAACAATTG GCGATTTGAG TACCCTTACA GCATCTGAAA TAAAAACTCT TCCTATCCGT 
TCTCCAAAAG TGTCTAACGT TAAAAAGGCT CTCAGAATAT ATCATGAGCA GCAGGTGAAG 
TCTCGTGGAC TAGAAGAGAT TCCAGTTTTT GATATTTCTG AAAAAACAAT AAATGGAATG 
GAAAATAAAT CTATCTCTCC TGATGAAGAA AGACTTGCCT CAGATTTAAT TGATCCTGTT 
GCTTTAGAAG CTCCGTTATC TAAAAATCTT GTGGCACAGA TTAGCGCACT TGCTCTTCAA 
CTAGATTCAG AAGATCTCTA TAATTATTCA GGAAGCCAGC TATTTGAAAT GCACGAGAAA 
CTTGGTAGCA TGGCAAACTC TATAATTAAA AATCTGCAGT CACGTTGGAG GTCACCAGCC 
CATGAAAATT CTTTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RIF1 ( XM_002685359.6 ) cDNA ORF clone, Bos taurus(cattle) -> XP_002685405.1 Bos taurus replication timing regulatory factor 1 (RIF1), transcript variant X1, mRNA.

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CloneID	OBa02875
Clone ID Related Accession (Same CDS sequence)	XM_010826492.2 , XM_002685359.6 , XM_002685359.5 , XM_024980692.1 , XM_024980695.1 , XM_010802076.2 , XM_615269.9
Accession Version	XM_002685359.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7398bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	telomere-associated protein RIF1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_002685359.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGCCG CGGATCAGAG CCCCCTGGCA CCGCTGTTGG AGACTTTGGA AGACCCTTCC 
GCCTCCCTTG GAGAGCAGAC CGACGCTTAC CTGACCCTGA CCAGTCGTAT GACTGGAGAA 
GATGGAAAAG AAATCATTAC AGAAATTGAG AAAAAACTTC CTCAGCTATT TAAAGTTTTA 
AAGACTCACA TTGCCAGTCA AAACTCAGAG CTCAGTAGTG CTGCTCTACA GGCCTTGGGG 
TTTTGCTTAT ATAATCCCAA AATTGCCTCT GGATTATCAG AGATAAATAT TCAAGAACTG 
CTTTCAAAAC TGAATGATAT TGTTAAAAGT TCAGATAAAA ATGTACGTAC TAGGGCACTT 
TGGGTGATAT CCAAGCAGAC GTTTCCCACT GAAGTCGTTG GCAAAGTAGT TTCTAGTATC 
ATTGACTCAC TAGAAATAGT ATTTAATAGA GGAGAGATGC TTTCTGCTGT TGTGGATTAT 
GAAGCATTAA ATGTCATTAT AAGACTAATT GAACAAGCCC CAGTTCAAAT GGGAGAAGAG 
TCAATTAGGT GGGCAAAATT GGTCATACCT TTACTGGTTC ATTCAGCCCA AAAAGTACAT 
TTGCGGGGAG CCACTGCTCT AGAGATGGGA ATGCCTTTGT TGCTTCAGAA ACAGCAAGAA 
ATAGCATCTA TCACAGAGCA GCTTATGACT ACCAAATTAC TTTCAGAACT CCAGAAACTG 
TTTATGAGTA AAAATGAGAC TTATGTGTTA AAATTGTGGC CTTTATTTGT CAAACTTCTT 
GGGAAGACCT TGCATCGAAG TGGGAGTTTC ATTAATTCTC TGTTACAGCT GGAAGAACTG 
GGATTTCGTA GTGGAGCACC CATGATTAAA AAAATAGCTT TTATTGCTTG GAAGAGTTTA 
ATAGATAATT TTGCTTTAAA TCCAGAAATA CTGTGTAGTG CAAAAAGACT GAAGTTGTTG 
ATGCAGCCTT TGAGTTCTAT CCATGTGAGA ACAGAAACTC TAGCGCTGAC AAAACTAGAA 
GTCTGGTGGT ATTTGTTGAT GAGACTAGGA CCTCATCTTC CTGCTAATTT TGAACAGGTT 
TGTGTGCCTC TGATTCAGAG CACAATAAGC ATTGATTCTA ATGCTTCACC TCAAGGGAGT 
TCATCTCGTG TAGCTACTAC TCCAGGTTTA AGCCCTATGA CCCCAGTACA CAAAGGTGCT 
TCTCCATATG GAACTCCTGT AACCCCTCGG ATGAACTTGA ATTCAAATTT TGGAATGGCC 
ACAATCCCCT CTATACAACT TTTGGGGCTT GAAATGTTGC TTCATTTTTT GTTGGGTCCA 
GAAGTCGTGA GTTTTGCTAA GCAAAACAAA CTTATACTGA GCCTAGAACC ACTTGAACAT 
CCATTAATCA GCAGCTCTTC TTTTTTCTCC AAACACTCAA ATACACTTAT CACTGCTGTT 
CATGATAGCT TTGTTGCAGT TGGAAAAGAT GCTTCTGATG TAGTTATTAA TGCTATTTGG 
AAGGAGTTAA TTAGCTTGGT GAAGTCAGTT ATTGAATCAG GTAACAAAAA AGAGAGACCA 
GGTTCTGAAG TTTTGACTCT CTTATTAAAA TCTTTGGAAA GCATAGTGAA TTCAGAAGTA 
TTTCCTGTAT TGAAAACACT GGTTCTCATG GAAATTACAA TTAAAGGACT TCCTCAGAAA 
GTATTAGGTT CTCCAGCATA TCAGGTTGCT AATATGGATA TTCTTAATGG AACCCCTGCT 
TTGTTCTTAA TTCAGTTAAC TTTCAATAAT AATCTATTGG AATGTGGTGT GGAAGACGAA 
AGGTTTTTTC TCAACCTGGA AACACTTGTG GGCTGTGTTC TTTCGGGTCC AACTTCACCA 
CTCGCTTTCA GTGACTCTGT CTTAAATGTT GTCAATCAAA ATGCAAAGCA GTTGGATAAC 
AAGGAGCATC TTTGGAGAAT GTGGAGTATT ATAGTCACCC CATTAACAGA ATTGATTAAT 
CAGACCAATG AAGTGAATCA AGGGGATGCC TTAGAACATA ATTTCAGTGC TATCTATGGT 
GCACTGATTT TGCCAATAAA CCATATTTTT CCAGCACAAA AAATTCCAGT GGTCACCATG 
AAGACCTTAC TTAAAACTTG GTCAGAATTA TATAGAGCAT TTGCTCGCTG TGCAGCTTTG 
GTGGCAACTG CGGAAGAGAA TTTATGCTGT GAGGAATTTT GTTCCAAGAT AATGTCCAGT 
TTGGAAGATG AAGGCCTTTC AAATTTATTG TTCTTGGATA GGATCACTCA TATTATTACT 
GTAATGGTTG ATTGCATCGA CTTTTCACCA TATAATATTA AATATCAACC CAAAACTAAA 
TCACCACAGA GACCTACAGA TTGGTCCAAA AAGAAGAAGG AACCTTTAGG GAAGTTGGCT 
TCTTTAATTA AACTTATTGT GAGAGTGATC TATTCTTTTC ACACACTGAG CCTCAAGGAA 
GTACATTCTG ATACTCTCCT CACTATTGGC AACTCAATCA CCAGCATTCT TTCCAATTTA 
CTTGGACATA TTTCTCTGCC TTCTATGATC CAGAAAATAT TTGCAGCTTT AACAAGACCT 
CTGGCATTAT TTTATGAAAA CTCAAAGCTT GATGAAGTTC CTAAAGTGTA TAGTTGTATG 
AACAACAAGT TAGAAAAGCT ACTAGGAGAT ATAATTGCTT GTCTACATGT CAGCTACACT 
GGAACTTACG ACAGTGAACT TCTTGAACAG ATCTCACCAT TATTATGCAT AATATTTTCA 
CACAAGAACA AACAGATAAG AAAACAAAGT GCTCAGTTCT GGAATGCCAC ATTTGCTAAA 
GTGACGATGT TGATTTATCC TGAGGAGTTA AAGCCAATAC TGAAACAAGC CAAACAAAAA 
TCTCTGCTTT TATTGCCTGG TTTGGAAAAT GTTGAAATTA TGGAGGAATC CAGTGGACCA 
TATTCAGATA TGACAGAAAA TTCCCAATTA AATATGAAGA TAAGTGGCAT GGAAAGAAAA 
TCAAGTGGAA AAAGAGATTC TTTTTTGGCA CAAACAAAGG ATACAAAAGA CAGTATGAAA 
CCACCAGCTA AATTGAAACT AGATTCTTCA ACTCCAAAAG CAAAGGGTGA AATGCCTTTG 
GAAGAAGAAA AGTCTACTGA CTTTGTGTTT ATACCTCCAG AAGTAAAAGA AACAAAGGAA 
AGAATACTAA CTGAACACCA GAAAGAAGTA CTCAAAACAA AGAGGTGTGA TATTCCTGCC 
ATGTATAATA ATCTGGATGT TTCACAAGAT ACTTTATTTT CTCAGTATAG TCAGGAAGAA 
TCTATGGAAA TTTCTACTTT AATTGAAAAA TCAAAAGAAG ACTCCAAGAT GATATTTAAG 
GAGGAACAAA AGGAGAATGA CATTGTCGTT CCTCAACATG TCATGGAAAA CTGCGGTATG 
GATGAACATC TTGAAAAGGC TTCCCTTTTG AATAATGAGT GTGGTTCTAT TGAGGAAACC 
AGTCCAAAAA TACTGAGCAG TAATAATGAT GCCAGAAAAA AAGCTTTAAT TGCTTCAAAT 
AAACCAACAG AATGTGCATC TACTACAGAA AATCCATCTG GTGTCAGCAG TAGCTCGGTT 
TCTATTGCCA CCATTTCTGG AGCTCCCTTA CAGCCTACAA GTCGAAGGCA ATCCTTTATT 
ACTTTGGAAA AGTTTGATGG TTCAGAAAAT AGACCTTTTA GTCCATCACC CTTGAATAAT 
ATGTCATCAA CTGTAATAGT GAAAAGTAAC CAGGAAGGCA TGGGTAAAAC AGATAATCCA 
TCAAAAGCAA AGAAGAGAGA AGTGGCTCTT TCCAAATCTG ACTCTGAAAA AATAACACAT 
GGGACTAAGA GATCAAGCCG AAGGTCGAGT AAATCTGAAC CAATAGGGAG TAAAAAGTCT 
AAACCCTTAA TGAGATCTGA ACAAGAGAAA AGTACTCAAG AATCTGTTGA TGGCACAGTA 
GCCTTAGAAA ATAACCCACC TGGCTTGCTT AATCAGACAG AATGTGTGTT AGATAATCAG 
GTTCATCTCT CTGAATCTAC AGTGGAGTTT GAGGATACAA TGCTTGAATC AACAGTGGAG 
AATACTGTAT TAGAAAACAA TACTGTAGAA GAGAGAACCT TAGAAGTTAA CTTGGAATCC 
AAAGAAAACA CACCCCCAGC CATAATAACG GATCAGACAG TAAATGAGGA TAGTCATATT 
CAGGTAACTC CAAATCAGAA AACCCTTAGG CGATCTTTAA GGCGACGTTC AGAAACAGCA 
GAACCAACCA CTGATAGCCA AGATAAAGAA AATAATCATC AAAAAAGGGA AAGACGTAAA 
GAAGAAGAAA AGACTCCGCA GAAGAGTCCG TTACATGTAA AAGATGATGT TTTACCTAAG 
CAAAAATTGA TTTCTGAACA AAGTGTACAG GGAAACTTAG TTGACAAAGG AAATAATTTA 
CATGAGAAGG CTTTTGGGGA GACCAGTGCT AATGCTGAAA TTGATGAAAA TAGAAGAAAG 
CCAGATCTTG AGAATACTAA ATCTGAAGGA GATGGTGTGC CGGACATTGC AGATAAATCC 
TCTGAGAAAC CAGTAAGGGG ACGAACACGA TACCAAACAA GAAGAGCATC CCAGGGTTTA 
CTTTCCAGCA TTGAAAACTC AGAATCTGAT AGTTCTGAGG CAAAAGAAGA AGGTTCTAAA 
AAGAAAAGAT CTGGAAAATG GAAGAACAAA AGCAGTGACA ATGTTGACAT GGAAGATCAA 
GAAGAGAAAA TGGTAAAACA GGAATGTATA AAAATTGAAA ATCAGGCACA TGATTATAAA 
GGGAATCCTG AAGTAGACAA AGATATAAAA TCTCAAATCT GTGAAAAAGG AGATGAAAGT 
ACTACTGTAA GTCTTCAAGA TTCTACCGTA TCTCCAGATT TATTACAAGT TTATGATGAT 
GTATTGAATA CTTCTGAGGG AGAAAGTAAA AGTAACAAAG ATGCAGACCA TTCCTTTTCA 
AATCCTTCTG TACCAGAATC AAATCTAAGG ACTAGAAATG CCAGTAAGAG GTTACATAAG 
CGAGATTCTA TAGAAAATAG CATGGGGGAA TCCTCAAAAA TAGGGACATC AGACATTTCT 
TTGCTTTCTG AAAAAACTCT TCAGACAGTA GAATGCCAGC ACAAGAGAAG TAGGAGGGTG 
AGGCGATCTA AAGGTTGTGA TTGTTGTGGA GAAAAATCAC AGTCTCAGGA AAAGTCATTT 
ACTGGGTTAA AGGATACAGA AAATTACGAT GTAAAAGTCA GTGAAACAAA AAAGACAGAT 
GGGGAAACAC CTGTATCTAT TTCAGAAACT TCGAAAGCTG ATCTGTCCTC GGAGGTTAAA 
CTTTTAGATG AGCATCATAG TGTGAATTTT CATTTGGATC TCAAGGAAGA GAATGATACT 
ACTAATGATT CATTAATTAT ATCTGAAACT GAGTTAAAAG AAAATACTAC TCAAAGCTTT 
CTTCCTTCTG AAATGGTAAA ATTTAAAGAA ACTTCTCGTA GGGATTCTGA GGAAGCAATA 
TCTCTAGAAA CCCAGGAGCC ATCTAATAAG AAATGTAAAA CTGTTGACCC ACATTTAGAT 
GTTTCCAAAG ATATTTCACT GAAATGTTTT TCTTTGGAGA CTAAAGAAGA AAAGTCAGAG 
GCCACTTCAA AAGAGAAATT GAGTACAGAG AACATTGTTA TTGTTGAATC AAATGCAGAA 
TCAAATCCAG AAGTAGATGC TATGGAATTG AATGTAGAAA ATGATCAGTC TGAAGCTGGC 
TTCTCTGAAC AAAAGAATGT CAAAACTGGT ATTCCAGGGA AAGAAGTAAC AGAGGAAAAC 
AGTGAAAGAA TGGATACACC TGAAAAACTG AAAGCCAATG AAGCAGTAAC TGAGGAATTT 
AATTCAGCTA CTGATTATTC GGATACTCCG ACATCTGTAA AAGTGAATGC TCAGGTAGAG 
ATCTCTGAAC AAATAGCGGT TGGGGAGCTA GGTGGAGGAA GTGATGAATC TGATCCAGGC 
TCATCTGAAG AAATGAACGC TGAAATGAAA AATTGTGAAA AAACAGCGGT TTGTGAGGTG 
ACTACTGAAT CTGACCACGT CACTGAAACC GAAGACGAAG ATGTAACTAC CACGGCTTCT 
CTGCCTATAG AAGTTGAAAC AAAGGAATTA GATGTAGAAG TCAATAACTT TGTTCCCGAC 
ACACTTGAGA TGATCACTGA AGAAGGAAAG GTTGACCCTA ACAAAACTGA AACGAATACT 
GAACCTAATA AATTGGAGGA AACACAGTTA GATGACAATC AGATGGTAGT GGAAAGTAAT 
GAAACTTTAC CGGAAGTTCA CCATACTTCA GAGAAAGTAG AGGAGATAGC ACAACCTCTG 
ACATCTGAAA CAGATATTTC TGGACTAATA TCAGAAGACA GTAATACATC TCCTCAAAAA 
TCGAAGGATA TTGATCCTTT ACTCATGTCA GCAAACGAGA GTCCTAGTGG CATGCACACA 
CGTTGTGTGT GGTCTCCTTT GGCTTCTCCA TCCACCAGCA TTTTAAAGAG AGGACTAAAA 
AGACCCCAAG AAGATGAAAT CTCGTCACCT GTCCACAAGG TTCGCCGTGT CTCCTTTGCA 
GATCCTATAT ACCAAGCAGG ATTGGCAGAT GACATTGATA GGCGATGCTC TATTGTTAGG 
TGCCATTCTT CAAATAGTTC TCTCATGGTA AAAAGTGTTA AAACTTCACC TACTGTGCAA 
TCTAAGCATA ATACCACTTC AGCCAAAGGA TTTCTGTCCC CAGGATCACG TAGCTCTAAA 
TTTAAGAGCT CAAAGAAGTG TTTAATTGCA GAAATGGCTA AGGAATCTGT GCCATGCCCA 
ACAGAAAGTG TTTACCCTGC TTTGGTGAAC TGTGTAGCAC CAGTTGAAAT CATTTTACCT 
CAGATTACAT CCAACATGTG GGCAAGAGGA CTGGGACAGC TCATTAGAGC CAAGAATATA 
AAAACAATTG GCGATTTGAG TACCCTTACA GCATCTGAAA TAAAAACTCT TCCTATCCGT 
TCTCCAAAAG TGTCTAACGT TAAAAAGGCT CTCAGAATAT ATCATGAGCA GCAGGTGAAG 
TCTCGTGGAC TAGAAGAGAT TCCAGTTTTT GATATTTCTG AAAAAACAAT AAATGGAATG 
GAAAATAAAT CTATCTCTCC TGATGAAGAA AGACTTGCCT CAGATTTAAT TGATCCTGTT 
GCTTTAGAAG CTCCGTTATC TAAAAATCTT GTGGCACAGA TTAGCGCACT TGCTCTTCAA 
CTAGATTCAG AAGATCTCTA TAATTATTCA GGAAGCCAGC TATTTGAAAT GCACGAGAAA 
CTTGGTAGCA TGGCAAACTC TATAATTAAA AATCTGCAGT CACGTTGGAG GTCACCAGCC 
CATGAAAATT CTTTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002685405.1
CDS	171..7568
Translation	MTAADQSPLAPLLETLEDPSASLGEQTDAYLTLTSRMTGEDGKEIITEIEKKLPQLFKVLKTHIASQNSELSSAALQALGFCLYNPKIASGLSEINIQELLSKLNDIVKSSDKNVRTRALWVISKQTFPTEVVGKVVSSIIDSLEIVFNRGEMLSAVVDYEALNVIIRLIEQAPVQMGEESIRWAKLVIPLLVHSAQKVHLRGATALEMGMPLLLQKQQEIASITEQLMTTKLLSELQKLFMSKNETYVLKLWPLFVKLLGKTLHRSGSFINSLLQLEELGFRSGAPMIKKIAFIAWKSLIDNFALNPEILCSAKRLKLLMQPLSSIHVRTETLALTKLEVWWYLLMRLGPHLPANFEQVCVPLIQSTISIDSNASPQGSSSRVATTPGLSPMTPVHKGASPYGTPVTPRMNLNSNFGMATIPSIQLLGLEMLLHFLLGPEVVSFAKQNKLILSLEPLEHPLISSSSFFSKHSNTLITAVHDSFVAVGKDASDVVINAIWKELISLVKSVIESGNKKERPGSEVLTLLLKSLESIVNSEVFPVLKTLVLMEITIKGLPQKVLGSPAYQVANMDILNGTPALFLIQLTFNNNLLECGVEDERFFLNLETLVGCVLSGPTSPLAFSDSVLNVVNQNAKQLDNKEHLWRMWSIIVTPLTELINQTNEVNQGDALEHNFSAIYGALILPINHIFPAQKIPVVTMKTLLKTWSELYRAFARCAALVATAEENLCCEEFCSKIMSSLEDEGLSNLLFLDRITHIITVMVDCIDFSPYNIKYQPKTKSPQRPTDWSKKKKEPLGKLASLIKLIVRVIYSFHTLSLKEVHSDTLLTIGNSITSILSNLLGHISLPSMIQKIFAALTRPLALFYENSKLDEVPKVYSCMNNKLEKLLGDIIACLHVSYTGTYDSELLEQISPLLCIIFSHKNKQIRKQSAQFWNATFAKVTMLIYPEELKPILKQAKQKSLLLLPGLENVEIMEESSGPYSDMTENSQLNMKISGMERKSSGKRDSFLAQTKDTKDSMKPPAKLKLDSSTPKAKGEMPLEEEKSTDFVFIPPEVKETKERILTEHQKEVLKTKRCDIPAMYNNLDVSQDTLFSQYSQEESMEISTLIEKSKEDSKMIFKEEQKENDIVVPQHVMENCGMDEHLEKASLLNNECGSIEETSPKILSSNNDARKKALIASNKPTECASTTENPSGVSSSSVSIATISGAPLQPTSRRQSFITLEKFDGSENRPFSPSPLNNMSSTVIVKSNQEGMGKTDNPSKAKKREVALSKSDSEKITHGTKRSSRRSSKSEPIGSKKSKPLMRSEQEKSTQESVDGTVALENNPPGLLNQTECVLDNQVHLSESTVEFEDTMLESTVENTVLENNTVEERTLEVNLESKENTPPAIITDQTVNEDSHIQVTPNQKTLRRSLRRRSETAEPTTDSQDKENNHQKRERRKEEEKTPQKSPLHVKDDVLPKQKLISEQSVQGNLVDKGNNLHEKAFGETSANAEIDENRRKPDLENTKSEGDGVPDIADKSSEKPVRGRTRYQTRRASQGLLSSIENSESDSSEAKEEGSKKKRSGKWKNKSSDNVDMEDQEEKMVKQECIKIENQAHDYKGNPEVDKDIKSQICEKGDESTTVSLQDSTVSPDLLQVYDDVLNTSEGESKSNKDADHSFSNPSVPESNLRTRNASKRLHKRDSIENSMGESSKIGTSDISLLSEKTLQTVECQHKRSRRVRRSKGCDCCGEKSQSQEKSFTGLKDTENYDVKVSETKKTDGETPVSISETSKADLSSEVKLLDEHHSVNFHLDLKEENDTTNDSLIISETELKENTTQSFLPSEMVKFKETSRRDSEEAISLETQEPSNKKCKTVDPHLDVSKDISLKCFSLETKEEKSEATSKEKLSTENIVIVESNAESNPEVDAMELNVENDQSEAGFSEQKNVKTGIPGKEVTEENSERMDTPEKLKANEAVTEEFNSATDYSDTPTSVKVNAQVEISEQIAVGELGGGSDESDPGSSEEMNAEMKNCEKTAVCEVTTESDHVTETEDEDVTTTASLPIEVETKELDVEVNNFVPDTLEMITEEGKVDPNKTETNTEPNKLEETQLDDNQMVVESNETLPEVHHTSEKVEEIAQPLTSETDISGLISEDSNTSPQKSKDIDPLLMSANESPSGMHTRCVWSPLASPSTSILKRGLKRPQEDEISSPVHKVRRVSFADPIYQAGLADDIDRRCSIVRCHSSNSSLMVKSVKTSPTVQSKHNTTSAKGFLSPGSRSSKFKSSKKCLIAEMAKESVPCPTESVYPALVNCVAPVEIILPQITSNMWARGLGQLIRAKNIKTIGDLSTLTASEIKTLPIRSPKVSNVKKALRIYHEQQVKSRGLEEIPVFDISEKTINGMENKSISPDEERLASDLIDPVALEAPLSKNLVAQISALALQLDSEDLYNYSGSQLFEMHEKLGSMANSIIKNLQSRWRSPAHENSF

Target ORF information:

RefSeq Version	XM_002685359.6
Organism	Bos taurus(cattle)
Definition	Bos taurus replication timing regulatory factor 1 (RIF1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002685359.6

ORF Insert Sequence:

ATGACGGCCG CGGATCAGAG CCCCCTGGCA CCGCTGTTGG AGACTTTGGA AGACCCTTCC 
GCCTCCCTTG GAGAGCAGAC CGACGCTTAC CTGACCCTGA CCAGTCGTAT GACTGGAGAA 
GATGGAAAAG AAATCATTAC AGAAATTGAG AAAAAACTTC CTCAGCTATT TAAAGTTTTA 
AAGACTCACA TTGCCAGTCA AAACTCAGAG CTCAGTAGTG CTGCTCTACA GGCCTTGGGG 
TTTTGCTTAT ATAATCCCAA AATTGCCTCT GGATTATCAG AGATAAATAT TCAAGAACTG 
CTTTCAAAAC TGAATGATAT TGTTAAAAGT TCAGATAAAA ATGTACGTAC TAGGGCACTT 
TGGGTGATAT CCAAGCAGAC GTTTCCCACT GAAGTCGTTG GCAAAGTAGT TTCTAGTATC 
ATTGACTCAC TAGAAATAGT ATTTAATAGA GGAGAGATGC TTTCTGCTGT TGTGGATTAT 
GAAGCATTAA ATGTCATTAT AAGACTAATT GAACAAGCCC CAGTTCAAAT GGGAGAAGAG 
TCAATTAGGT GGGCAAAATT GGTCATACCT TTACTGGTTC ATTCAGCCCA AAAAGTACAT 
TTGCGGGGAG CCACTGCTCT AGAGATGGGA ATGCCTTTGT TGCTTCAGAA ACAGCAAGAA 
ATAGCATCTA TCACAGAGCA GCTTATGACT ACCAAATTAC TTTCAGAACT CCAGAAACTG 
TTTATGAGTA AAAATGAGAC TTATGTGTTA AAATTGTGGC CTTTATTTGT CAAACTTCTT 
GGGAAGACCT TGCATCGAAG TGGGAGTTTC ATTAATTCTC TGTTACAGCT GGAAGAACTG 
GGATTTCGTA GTGGAGCACC CATGATTAAA AAAATAGCTT TTATTGCTTG GAAGAGTTTA 
ATAGATAATT TTGCTTTAAA TCCAGAAATA CTGTGTAGTG CAAAAAGACT GAAGTTGTTG 
ATGCAGCCTT TGAGTTCTAT CCATGTGAGA ACAGAAACTC TAGCGCTGAC AAAACTAGAA 
GTCTGGTGGT ATTTGTTGAT GAGACTAGGA CCTCATCTTC CTGCTAATTT TGAACAGGTT 
TGTGTGCCTC TGATTCAGAG CACAATAAGC ATTGATTCTA ATGCTTCACC TCAAGGGAGT 
TCATCTCGTG TAGCTACTAC TCCAGGTTTA AGCCCTATGA CCCCAGTACA CAAAGGTGCT 
TCTCCATATG GAACTCCTGT AACCCCTCGG ATGAACTTGA ATTCAAATTT TGGAATGGCC 
ACAATCCCCT CTATACAACT TTTGGGGCTT GAAATGTTGC TTCATTTTTT GTTGGGTCCA 
GAAGTCGTGA GTTTTGCTAA GCAAAACAAA CTTATACTGA GCCTAGAACC ACTTGAACAT 
CCATTAATCA GCAGCTCTTC TTTTTTCTCC AAACACTCAA ATACACTTAT CACTGCTGTT 
CATGATAGCT TTGTTGCAGT TGGAAAAGAT GCTTCTGATG TAGTTATTAA TGCTATTTGG 
AAGGAGTTAA TTAGCTTGGT GAAGTCAGTT ATTGAATCAG GTAACAAAAA AGAGAGACCA 
GGTTCTGAAG TTTTGACTCT CTTATTAAAA TCTTTGGAAA GCATAGTGAA TTCAGAAGTA 
TTTCCTGTAT TGAAAACACT GGTTCTCATG GAAATTACAA TTAAAGGACT TCCTCAGAAA 
GTATTAGGTT CTCCAGCATA TCAGGTTGCT AATATGGATA TTCTTAATGG AACCCCTGCT 
TTGTTCTTAA TTCAGTTAAC TTTCAATAAT AATCTATTGG AATGTGGTGT GGAAGACGAA 
AGGTTTTTTC TCAACCTGGA AACACTTGTG GGCTGTGTTC TTTCGGGTCC AACTTCACCA 
CTCGCTTTCA GTGACTCTGT CTTAAATGTT GTCAATCAAA ATGCAAAGCA GTTGGATAAC 
AAGGAGCATC TTTGGAGAAT GTGGAGTATT ATAGTCACCC CATTAACAGA ATTGATTAAT 
CAGACCAATG AAGTGAATCA AGGGGATGCC TTAGAACATA ATTTCAGTGC TATCTATGGT 
GCACTGATTT TGCCAATAAA CCATATTTTT CCAGCACAAA AAATTCCAGT GGTCACCATG 
AAGACCTTAC TTAAAACTTG GTCAGAATTA TATAGAGCAT TTGCTCGCTG TGCAGCTTTG 
GTGGCAACTG CGGAAGAGAA TTTATGCTGT GAGGAATTTT GTTCCAAGAT AATGTCCAGT 
TTGGAAGATG AAGGCCTTTC AAATTTATTG TTCTTGGATA GGATCACTCA TATTATTACT 
GTAATGGTTG ATTGCATCGA CTTTTCACCA TATAATATTA AATATCAACC CAAAACTAAA 
TCACCACAGA GACCTACAGA TTGGTCCAAA AAGAAGAAGG AACCTTTAGG GAAGTTGGCT 
TCTTTAATTA AACTTATTGT GAGAGTGATC TATTCTTTTC ACACACTGAG CCTCAAGGAA 
GTACATTCTG ATACTCTCCT CACTATTGGC AACTCAATCA CCAGCATTCT TTCCAATTTA 
CTTGGACATA TTTCTCTGCC TTCTATGATC CAGAAAATAT TTGCAGCTTT AACAAGACCT 
CTGGCATTAT TTTATGAAAA CTCAAAGCTT GATGAAGTTC CTAAAGTGTA TAGTTGTATG 
AACAACAAGT TAGAAAAGCT ACTAGGAGAT ATAATTGCTT GTCTACATGT CAGCTACACT 
GGAACTTACG ACAGTGAACT TCTTGAACAG ATCTCACCAT TATTATGCAT AATATTTTCA 
CACAAGAACA AACAGATAAG AAAACAAAGT GCTCAGTTCT GGAATGCCAC ATTTGCTAAA 
GTGACGATGT TGATTTATCC TGAGGAGTTA AAGCCAATAC TGAAACAAGC CAAACAAAAA 
TCTCTGCTTT TATTGCCTGG TTTGGAAAAT GTTGAAATTA TGGAGGAATC CAGTGGACCA 
TATTCAGATA TGACAGAAAA TTCCCAATTA AATATGAAGA TAAGTGGCAT GGAAAGAAAA 
TCAAGTGGAA AAAGAGATTC TTTTTTGGCA CAAACAAAGG ATACAAAAGA CAGTATGAAA 
CCACCAGCTA AATTGAAACT AGATTCTTCA ACTCCAAAAG CAAAGGGTGA AATGCCTTTG 
GAAGAAGAAA AGTCTACTGA CTTTGTGTTT ATACCTCCAG AAGTAAAAGA AACAAAGGAA 
AGAATACTAA CTGAACACCA GAAAGAAGTA CTCAAAACAA AGAGGTGTGA TATTCCTGCC 
ATGTATAATA ATCTGGATGT TTCACAAGAT ACTTTATTTT CTCAGTATAG TCAGGAAGAA 
TCTATGGAAA TTTCTACTTT AATTGAAAAA TCAAAAGAAG ACTCCAAGAT GATATTTAAG 
GAGGAACAAA AGGAGAATGA CATTGTCGTT CCTCAACATG TCATGGAAAA CTGCGGTATG 
GATGAACATC TTGAAAAGGC TTCCCTTTTG AATAATGAGT GTGGTTCTAT TGAGGAAACC 
AGTCCAAAAA TACTGAGCAG TAATAATGAT GCCAGAAAAA AAGCTTTAAT TGCTTCAAAT 
AAACCAACAG AATGTGCATC TACTACAGAA AATCCATCTG GTGTCAGCAG TAGCTCGGTT 
TCTATTGCCA CCATTTCTGG AGCTCCCTTA CAGCCTACAA GTCGAAGGCA ATCCTTTATT 
ACTTTGGAAA AGTTTGATGG TTCAGAAAAT AGACCTTTTA GTCCATCACC CTTGAATAAT 
ATGTCATCAA CTGTAATAGT GAAAAGTAAC CAGGAAGGCA TGGGTAAAAC AGATAATCCA 
TCAAAAGCAA AGAAGAGAGA AGTGGCTCTT TCCAAATCTG ACTCTGAAAA AATAACACAT 
GGGACTAAGA GATCAAGCCG AAGGTCGAGT AAATCTGAAC CAATAGGGAG TAAAAAGTCT 
AAACCCTTAA TGAGATCTGA ACAAGAGAAA AGTACTCAAG AATCTGTTGA TGGCACAGTA 
GCCTTAGAAA ATAACCCACC TGGCTTGCTT AATCAGACAG AATGTGTGTT AGATAATCAG 
GTTCATCTCT CTGAATCTAC AGTGGAGTTT GAGGATACAA TGCTTGAATC AACAGTGGAG 
AATACTGTAT TAGAAAACAA TACTGTAGAA GAGAGAACCT TAGAAGTTAA CTTGGAATCC 
AAAGAAAACA CACCCCCAGC CATAATAACG GATCAGACAG TAAATGAGGA TAGTCATATT 
CAGGTAACTC CAAATCAGAA AACCCTTAGG CGATCTTTAA GGCGACGTTC AGAAACAGCA 
GAACCAACCA CTGATAGCCA AGATAAAGAA AATAATCATC AAAAAAGGGA AAGACGTAAA 
GAAGAAGAAA AGACTCCGCA GAAGAGTCCG TTACATGTAA AAGATGATGT TTTACCTAAG 
CAAAAATTGA TTTCTGAACA AAGTGTACAG GGAAACTTAG TTGACAAAGG AAATAATTTA 
CATGAGAAGG CTTTTGGGGA GACCAGTGCT AATGCTGAAA TTGATGAAAA TAGAAGAAAG 
CCAGATCTTG AGAATACTAA ATCTGAAGGA GATGGTGTGC CGGACATTGC AGATAAATCC 
TCTGAGAAAC CAGTAAGGGG ACGAACACGA TACCAAACAA GAAGAGCATC CCAGGGTTTA 
CTTTCCAGCA TTGAAAACTC AGAATCTGAT AGTTCTGAGG CAAAAGAAGA AGGTTCTAAA 
AAGAAAAGAT CTGGAAAATG GAAGAACAAA AGCAGTGACA ATGTTGACAT GGAAGATCAA 
GAAGAGAAAA TGGTAAAACA GGAATGTATA AAAATTGAAA ATCAGGCACA TGATTATAAA 
GGGAATCCTG AAGTAGACAA AGATATAAAA TCTCAAATCT GTGAAAAAGG AGATGAAAGT 
ACTACTGTAA GTCTTCAAGA TTCTACCGTA TCTCCAGATT TATTACAAGT TTATGATGAT 
GTATTGAATA CTTCTGAGGG AGAAAGTAAA AGTAACAAAG ATGCAGACCA TTCCTTTTCA 
AATCCTTCTG TACCAGAATC AAATCTAAGG ACTAGAAATG CCAGTAAGAG GTTACATAAG 
CGAGATTCTA TAGAAAATAG CATGGGGGAA TCCTCAAAAA TAGGGACATC AGACATTTCT 
TTGCTTTCTG AAAAAACTCT TCAGACAGTA GAATGCCAGC ACAAGAGAAG TAGGAGGGTG 
AGGCGATCTA AAGGTTGTGA TTGTTGTGGA GAAAAATCAC AGTCTCAGGA AAAGTCATTT 
ACTGGGTTAA AGGATACAGA AAATTACGAT GTAAAAGTCA GTGAAACAAA AAAGACAGAT 
GGGGAAACAC CTGTATCTAT TTCAGAAACT TCGAAAGCTG ATCTGTCCTC GGAGGTTAAA 
CTTTTAGATG AGCATCATAG TGTGAATTTT CATTTGGATC TCAAGGAAGA GAATGATACT 
ACTAATGATT CATTAATTAT ATCTGAAACT GAGTTAAAAG AAAATACTAC TCAAAGCTTT 
CTTCCTTCTG AAATGGTAAA ATTTAAAGAA ACTTCTCGTA GGGATTCTGA GGAAGCAATA 
TCTCTAGAAA CCCAGGAGCC ATCTAATAAG AAATGTAAAA CTGTTGACCC ACATTTAGAT 
GTTTCCAAAG ATATTTCACT GAAATGTTTT TCTTTGGAGA CTAAAGAAGA AAAGTCAGAG 
GCCACTTCAA AAGAGAAATT GAGTACAGAG AACATTGTTA TTGTTGAATC AAATGCAGAA 
TCAAATCCAG AAGTAGATGC TATGGAATTG AATGTAGAAA ATGATCAGTC TGAAGCTGGC 
TTCTCTGAAC AAAAGAATGT CAAAACTGGT ATTCCAGGGA AAGAAGTAAC AGAGGAAAAC 
AGTGAAAGAA TGGATACACC TGAAAAACTG AAAGCCAATG AAGCAGTAAC TGAGGAATTT 
AATTCAGCTA CTGATTATTC GGATACTCCG ACATCTGTAA AAGTGAATGC TCAGGTAGAG 
ATCTCTGAAC AAATAGCGGT TGGGGAGCTA GGTGGAGGAA GTGATGAATC TGATCCAGGC 
TCATCTGAAG AAATGAACGC TGAAATGAAA AATTGTGAAA AAACAGCGGT TTGTGAGGTG 
ACTACTGAAT CTGACCACGT CACTGAAACC GAAGACGAAG ATGTAACTAC CACGGCTTCT 
CTGCCTATAG AAGTTGAAAC AAAGGAATTA GATGTAGAAG TCAATAACTT TGTTCCCGAC 
ACACTTGAGA TGATCACTGA AGAAGGAAAG GTTGACCCTA ACAAAACTGA AACGAATACT 
GAACCTAATA AATTGGAGGA AACACAGTTA GATGACAATC AGATGGTAGT GGAAAGTAAT 
GAAACTTTAC CGGAAGTTCA CCATACTTCA GAGAAAGTAG AGGAGATAGC ACAACCTCTG 
ACATCTGAAA CAGATATTTC TGGACTAATA TCAGAAGACA GTAATACATC TCCTCAAAAA 
TCGAAGGATA TTGATCCTTT ACTCATGTCA GCAAACGAGA GTCCTAGTGG CATGCACACA 
CGTTGTGTGT GGTCTCCTTT GGCTTCTCCA TCCACCAGCA TTTTAAAGAG AGGACTAAAA 
AGACCCCAAG AAGATGAAAT CTCGTCACCT GTCCACAAGG TTCGCCGTGT CTCCTTTGCA 
GATCCTATAT ACCAAGCAGG ATTGGCAGAT GACATTGATA GGCGATGCTC TATTGTTAGG 
TGCCATTCTT CAAATAGTTC TCTCATGGTA AAAAGTGTTA AAACTTCACC TACTGTGCAA 
TCTAAGCATA ATACCACTTC AGCCAAAGGA TTTCTGTCCC CAGGATCACG TAGCTCTAAA 
TTTAAGAGCT CAAAGAAGTG TTTAATTGCA GAAATGGCTA AGGAATCTGT GCCATGCCCA 
ACAGAAAGTG TTTACCCTGC TTTGGTGAAC TGTGTAGCAC CAGTTGAAAT CATTTTACCT 
CAGATTACAT CCAACATGTG GGCAAGAGGA CTGGGACAGC TCATTAGAGC CAAGAATATA 
AAAACAATTG GCGATTTGAG TACCCTTACA GCATCTGAAA TAAAAACTCT TCCTATCCGT 
TCTCCAAAAG TGTCTAACGT TAAAAAGGCT CTCAGAATAT ATCATGAGCA GCAGGTGAAG 
TCTCGTGGAC TAGAAGAGAT TCCAGTTTTT GATATTTCTG AAAAAACAAT AAATGGAATG 
GAAAATAAAT CTATCTCTCC TGATGAAGAA AGACTTGCCT CAGATTTAAT TGATCCTGTT 
GCTTTAGAAG CTCCGTTATC TAAAAATCTT GTGGCACAGA TTAGCGCACT TGCTCTTCAA 
CTAGATTCAG AAGATCTCTA TAATTATTCA GGAAGCCAGC TATTTGAAAT GCACGAGAAA 
CTTGGTAGCA TGGCAAACTC TATAATTAAA AATCTGCAGT CACGTTGGAG GTCACCAGCC 
CATGAAAATT CTTTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RIF1 ( XM_002685359.5 ) cDNA ORF clone, Bos taurus(cattle) -> XP_002685405.1 Bos taurus replication timing regulatory factor 1 (RIF1), transcript variant X1, mRNA.

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CloneID	OBa02875
Clone ID Related Accession (Same CDS sequence)	XM_010826492.2 , XM_002685359.6 , XM_002685359.5 , XM_024980692.1 , XM_024980695.1 , XM_010802076.2 , XM_615269.9
Accession Version	XM_002685359.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7398bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	telomere-associated protein RIF1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103838.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_002685359.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGCCG CGGATCAGAG CCCCCTGGCA CCGCTGTTGG AGACTTTGGA AGACCCTTCC 
GCCTCCCTTG GAGAGCAGAC CGACGCTTAC CTGACCCTGA CCAGTCGTAT GACTGGAGAA 
GATGGAAAAG AAATCATTAC AGAAATTGAG AAAAAACTTC CTCAGCTATT TAAAGTTTTA 
AAGACTCACA TTGCCAGTCA AAACTCAGAG CTCAGTAGTG CTGCTCTACA GGCCTTGGGG 
TTTTGCTTAT ATAATCCCAA AATTGCCTCT GGATTATCAG AGATAAATAT TCAAGAACTG 
CTTTCAAAAC TGAATGATAT TGTTAAAAGT TCAGATAAAA ATGTACGTAC TAGGGCACTT 
TGGGTGATAT CCAAGCAGAC GTTTCCCACT GAAGTCGTTG GCAAAGTAGT TTCTAGTATC 
ATTGACTCAC TAGAAATAGT ATTTAATAGA GGAGAGATGC TTTCTGCTGT TGTGGATTAT 
GAAGCATTAA ATGTCATTAT AAGACTAATT GAACAAGCCC CAGTTCAAAT GGGAGAAGAG 
TCAATTAGGT GGGCAAAATT GGTCATACCT TTACTGGTTC ATTCAGCCCA AAAAGTACAT 
TTGCGGGGAG CCACTGCTCT AGAGATGGGA ATGCCTTTGT TGCTTCAGAA ACAGCAAGAA 
ATAGCATCTA TCACAGAGCA GCTTATGACT ACCAAATTAC TTTCAGAACT CCAGAAACTG 
TTTATGAGTA AAAATGAGAC TTATGTGTTA AAATTGTGGC CTTTATTTGT CAAACTTCTT 
GGGAAGACCT TGCATCGAAG TGGGAGTTTC ATTAATTCTC TGTTACAGCT GGAAGAACTG 
GGATTTCGTA GTGGAGCACC CATGATTAAA AAAATAGCTT TTATTGCTTG GAAGAGTTTA 
ATAGATAATT TTGCTTTAAA TCCAGAAATA CTGTGTAGTG CAAAAAGACT GAAGTTGTTG 
ATGCAGCCTT TGAGTTCTAT CCATGTGAGA ACAGAAACTC TAGCGCTGAC AAAACTAGAA 
GTCTGGTGGT ATTTGTTGAT GAGACTAGGA CCTCATCTTC CTGCTAATTT TGAACAGGTT 
TGTGTGCCTC TGATTCAGAG CACAATAAGC ATTGATTCTA ATGCTTCACC TCAAGGGAGT 
TCATCTCGTG TAGCTACTAC TCCAGGTTTA AGCCCTATGA CCCCAGTACA CAAAGGTGCT 
TCTCCATATG GAACTCCTGT AACCCCTCGG ATGAACTTGA ATTCAAATTT TGGAATGGCC 
ACAATCCCCT CTATACAACT TTTGGGGCTT GAAATGTTGC TTCATTTTTT GTTGGGTCCA 
GAAGTCGTGA GTTTTGCTAA GCAAAACAAA CTTATACTGA GCCTAGAACC ACTTGAACAT 
CCATTAATCA GCAGCTCTTC TTTTTTCTCC AAACACTCAA ATACACTTAT CACTGCTGTT 
CATGATAGCT TTGTTGCAGT TGGAAAAGAT GCTTCTGATG TAGTTATTAA TGCTATTTGG 
AAGGAGTTAA TTAGCTTGGT GAAGTCAGTT ATTGAATCAG GTAACAAAAA AGAGAGACCA 
GGTTCTGAAG TTTTGACTCT CTTATTAAAA TCTTTGGAAA GCATAGTGAA TTCAGAAGTA 
TTTCCTGTAT TGAAAACACT GGTTCTCATG GAAATTACAA TTAAAGGACT TCCTCAGAAA 
GTATTAGGTT CTCCAGCATA TCAGGTTGCT AATATGGATA TTCTTAATGG AACCCCTGCT 
TTGTTCTTAA TTCAGTTAAC TTTCAATAAT AATCTATTGG AATGTGGTGT GGAAGACGAA 
AGGTTTTTTC TCAACCTGGA AACACTTGTG GGCTGTGTTC TTTCGGGTCC AACTTCACCA 
CTCGCTTTCA GTGACTCTGT CTTAAATGTT GTCAATCAAA ATGCAAAGCA GTTGGATAAC 
AAGGAGCATC TTTGGAGAAT GTGGAGTATT ATAGTCACCC CATTAACAGA ATTGATTAAT 
CAGACCAATG AAGTGAATCA AGGGGATGCC TTAGAACATA ATTTCAGTGC TATCTATGGT 
GCACTGATTT TGCCAATAAA CCATATTTTT CCAGCACAAA AAATTCCAGT GGTCACCATG 
AAGACCTTAC TTAAAACTTG GTCAGAATTA TATAGAGCAT TTGCTCGCTG TGCAGCTTTG 
GTGGCAACTG CGGAAGAGAA TTTATGCTGT GAGGAATTTT GTTCCAAGAT AATGTCCAGT 
TTGGAAGATG AAGGCCTTTC AAATTTATTG TTCTTGGATA GGATCACTCA TATTATTACT 
GTAATGGTTG ATTGCATCGA CTTTTCACCA TATAATATTA AATATCAACC CAAAACTAAA 
TCACCACAGA GACCTACAGA TTGGTCCAAA AAGAAGAAGG AACCTTTAGG GAAGTTGGCT 
TCTTTAATTA AACTTATTGT GAGAGTGATC TATTCTTTTC ACACACTGAG CCTCAAGGAA 
GTACATTCTG ATACTCTCCT CACTATTGGC AACTCAATCA CCAGCATTCT TTCCAATTTA 
CTTGGACATA TTTCTCTGCC TTCTATGATC CAGAAAATAT TTGCAGCTTT AACAAGACCT 
CTGGCATTAT TTTATGAAAA CTCAAAGCTT GATGAAGTTC CTAAAGTGTA TAGTTGTATG 
AACAACAAGT TAGAAAAGCT ACTAGGAGAT ATAATTGCTT GTCTACATGT CAGCTACACT 
GGAACTTACG ACAGTGAACT TCTTGAACAG ATCTCACCAT TATTATGCAT AATATTTTCA 
CACAAGAACA AACAGATAAG AAAACAAAGT GCTCAGTTCT GGAATGCCAC ATTTGCTAAA 
GTGACGATGT TGATTTATCC TGAGGAGTTA AAGCCAATAC TGAAACAAGC CAAACAAAAA 
TCTCTGCTTT TATTGCCTGG TTTGGAAAAT GTTGAAATTA TGGAGGAATC CAGTGGACCA 
TATTCAGATA TGACAGAAAA TTCCCAATTA AATATGAAGA TAAGTGGCAT GGAAAGAAAA 
TCAAGTGGAA AAAGAGATTC TTTTTTGGCA CAAACAAAGG ATACAAAAGA CAGTATGAAA 
CCACCAGCTA AATTGAAACT AGATTCTTCA ACTCCAAAAG CAAAGGGTGA AATGCCTTTG 
GAAGAAGAAA AGTCTACTGA CTTTGTGTTT ATACCTCCAG AAGTAAAAGA AACAAAGGAA 
AGAATACTAA CTGAACACCA GAAAGAAGTA CTCAAAACAA AGAGGTGTGA TATTCCTGCC 
ATGTATAATA ATCTGGATGT TTCACAAGAT ACTTTATTTT CTCAGTATAG TCAGGAAGAA 
TCTATGGAAA TTTCTACTTT AATTGAAAAA TCAAAAGAAG ACTCCAAGAT GATATTTAAG 
GAGGAACAAA AGGAGAATGA CATTGTCGTT CCTCAACATG TCATGGAAAA CTGCGGTATG 
GATGAACATC TTGAAAAGGC TTCCCTTTTG AATAATGAGT GTGGTTCTAT TGAGGAAACC 
AGTCCAAAAA TACTGAGCAG TAATAATGAT GCCAGAAAAA AAGCTTTAAT TGCTTCAAAT 
AAACCAACAG AATGTGCATC TACTACAGAA AATCCATCTG GTGTCAGCAG TAGCTCGGTT 
TCTATTGCCA CCATTTCTGG AGCTCCCTTA CAGCCTACAA GTCGAAGGCA ATCCTTTATT 
ACTTTGGAAA AGTTTGATGG TTCAGAAAAT AGACCTTTTA GTCCATCACC CTTGAATAAT 
ATGTCATCAA CTGTAATAGT GAAAAGTAAC CAGGAAGGCA TGGGTAAAAC AGATAATCCA 
TCAAAAGCAA AGAAGAGAGA AGTGGCTCTT TCCAAATCTG ACTCTGAAAA AATAACACAT 
GGGACTAAGA GATCAAGCCG AAGGTCGAGT AAATCTGAAC CAATAGGGAG TAAAAAGTCT 
AAACCCTTAA TGAGATCTGA ACAAGAGAAA AGTACTCAAG AATCTGTTGA TGGCACAGTA 
GCCTTAGAAA ATAACCCACC TGGCTTGCTT AATCAGACAG AATGTGTGTT AGATAATCAG 
GTTCATCTCT CTGAATCTAC AGTGGAGTTT GAGGATACAA TGCTTGAATC AACAGTGGAG 
AATACTGTAT TAGAAAACAA TACTGTAGAA GAGAGAACCT TAGAAGTTAA CTTGGAATCC 
AAAGAAAACA CACCCCCAGC CATAATAACG GATCAGACAG TAAATGAGGA TAGTCATATT 
CAGGTAACTC CAAATCAGAA AACCCTTAGG CGATCTTTAA GGCGACGTTC AGAAACAGCA 
GAACCAACCA CTGATAGCCA AGATAAAGAA AATAATCATC AAAAAAGGGA AAGACGTAAA 
GAAGAAGAAA AGACTCCGCA GAAGAGTCCG TTACATGTAA AAGATGATGT TTTACCTAAG 
CAAAAATTGA TTTCTGAACA AAGTGTACAG GGAAACTTAG TTGACAAAGG AAATAATTTA 
CATGAGAAGG CTTTTGGGGA GACCAGTGCT AATGCTGAAA TTGATGAAAA TAGAAGAAAG 
CCAGATCTTG AGAATACTAA ATCTGAAGGA GATGGTGTGC CGGACATTGC AGATAAATCC 
TCTGAGAAAC CAGTAAGGGG ACGAACACGA TACCAAACAA GAAGAGCATC CCAGGGTTTA 
CTTTCCAGCA TTGAAAACTC AGAATCTGAT AGTTCTGAGG CAAAAGAAGA AGGTTCTAAA 
AAGAAAAGAT CTGGAAAATG GAAGAACAAA AGCAGTGACA ATGTTGACAT GGAAGATCAA 
GAAGAGAAAA TGGTAAAACA GGAATGTATA AAAATTGAAA ATCAGGCACA TGATTATAAA 
GGGAATCCTG AAGTAGACAA AGATATAAAA TCTCAAATCT GTGAAAAAGG AGATGAAAGT 
ACTACTGTAA GTCTTCAAGA TTCTACCGTA TCTCCAGATT TATTACAAGT TTATGATGAT 
GTATTGAATA CTTCTGAGGG AGAAAGTAAA AGTAACAAAG ATGCAGACCA TTCCTTTTCA 
AATCCTTCTG TACCAGAATC AAATCTAAGG ACTAGAAATG CCAGTAAGAG GTTACATAAG 
CGAGATTCTA TAGAAAATAG CATGGGGGAA TCCTCAAAAA TAGGGACATC AGACATTTCT 
TTGCTTTCTG AAAAAACTCT TCAGACAGTA GAATGCCAGC ACAAGAGAAG TAGGAGGGTG 
AGGCGATCTA AAGGTTGTGA TTGTTGTGGA GAAAAATCAC AGTCTCAGGA AAAGTCATTT 
ACTGGGTTAA AGGATACAGA AAATTACGAT GTAAAAGTCA GTGAAACAAA AAAGACAGAT 
GGGGAAACAC CTGTATCTAT TTCAGAAACT TCGAAAGCTG ATCTGTCCTC GGAGGTTAAA 
CTTTTAGATG AGCATCATAG TGTGAATTTT CATTTGGATC TCAAGGAAGA GAATGATACT 
ACTAATGATT CATTAATTAT ATCTGAAACT GAGTTAAAAG AAAATACTAC TCAAAGCTTT 
CTTCCTTCTG AAATGGTAAA ATTTAAAGAA ACTTCTCGTA GGGATTCTGA GGAAGCAATA 
TCTCTAGAAA CCCAGGAGCC ATCTAATAAG AAATGTAAAA CTGTTGACCC ACATTTAGAT 
GTTTCCAAAG ATATTTCACT GAAATGTTTT TCTTTGGAGA CTAAAGAAGA AAAGTCAGAG 
GCCACTTCAA AAGAGAAATT GAGTACAGAG AACATTGTTA TTGTTGAATC AAATGCAGAA 
TCAAATCCAG AAGTAGATGC TATGGAATTG AATGTAGAAA ATGATCAGTC TGAAGCTGGC 
TTCTCTGAAC AAAAGAATGT CAAAACTGGT ATTCCAGGGA AAGAAGTAAC AGAGGAAAAC 
AGTGAAAGAA TGGATACACC TGAAAAACTG AAAGCCAATG AAGCAGTAAC TGAGGAATTT 
AATTCAGCTA CTGATTATTC GGATACTCCG ACATCTGTAA AAGTGAATGC TCAGGTAGAG 
ATCTCTGAAC AAATAGCGGT TGGGGAGCTA GGTGGAGGAA GTGATGAATC TGATCCAGGC 
TCATCTGAAG AAATGAACGC TGAAATGAAA AATTGTGAAA AAACAGCGGT TTGTGAGGTG 
ACTACTGAAT CTGACCACGT CACTGAAACC GAAGACGAAG ATGTAACTAC CACGGCTTCT 
CTGCCTATAG AAGTTGAAAC AAAGGAATTA GATGTAGAAG TCAATAACTT TGTTCCCGAC 
ACACTTGAGA TGATCACTGA AGAAGGAAAG GTTGACCCTA ACAAAACTGA AACGAATACT 
GAACCTAATA AATTGGAGGA AACACAGTTA GATGACAATC AGATGGTAGT GGAAAGTAAT 
GAAACTTTAC CGGAAGTTCA CCATACTTCA GAGAAAGTAG AGGAGATAGC ACAACCTCTG 
ACATCTGAAA CAGATATTTC TGGACTAATA TCAGAAGACA GTAATACATC TCCTCAAAAA 
TCGAAGGATA TTGATCCTTT ACTCATGTCA GCAAACGAGA GTCCTAGTGG CATGCACACA 
CGTTGTGTGT GGTCTCCTTT GGCTTCTCCA TCCACCAGCA TTTTAAAGAG AGGACTAAAA 
AGACCCCAAG AAGATGAAAT CTCGTCACCT GTCCACAAGG TTCGCCGTGT CTCCTTTGCA 
GATCCTATAT ACCAAGCAGG ATTGGCAGAT GACATTGATA GGCGATGCTC TATTGTTAGG 
TGCCATTCTT CAAATAGTTC TCTCATGGTA AAAAGTGTTA AAACTTCACC TACTGTGCAA 
TCTAAGCATA ATACCACTTC AGCCAAAGGA TTTCTGTCCC CAGGATCACG TAGCTCTAAA 
TTTAAGAGCT CAAAGAAGTG TTTAATTGCA GAAATGGCTA AGGAATCTGT GCCATGCCCA 
ACAGAAAGTG TTTACCCTGC TTTGGTGAAC TGTGTAGCAC CAGTTGAAAT CATTTTACCT 
CAGATTACAT CCAACATGTG GGCAAGAGGA CTGGGACAGC TCATTAGAGC CAAGAATATA 
AAAACAATTG GCGATTTGAG TACCCTTACA GCATCTGAAA TAAAAACTCT TCCTATCCGT 
TCTCCAAAAG TGTCTAACGT TAAAAAGGCT CTCAGAATAT ATCATGAGCA GCAGGTGAAG 
TCTCGTGGAC TAGAAGAGAT TCCAGTTTTT GATATTTCTG AAAAAACAAT AAATGGAATG 
GAAAATAAAT CTATCTCTCC TGATGAAGAA AGACTTGCCT CAGATTTAAT TGATCCTGTT 
GCTTTAGAAG CTCCGTTATC TAAAAATCTT GTGGCACAGA TTAGCGCACT TGCTCTTCAA 
CTAGATTCAG AAGATCTCTA TAATTATTCA GGAAGCCAGC TATTTGAAAT GCACGAGAAA 
CTTGGTAGCA TGGCAAACTC TATAATTAAA AATCTGCAGT CACGTTGGAG GTCACCAGCC 
CATGAAAATT CTTTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002685405.1
CDS	146..7543
Translation	MTAADQSPLAPLLETLEDPSASLGEQTDAYLTLTSRMTGEDGKEIITEIEKKLPQLFKVLKTHIASQNSELSSAALQALGFCLYNPKIASGLSEINIQELLSKLNDIVKSSDKNVRTRALWVISKQTFPTEVVGKVVSSIIDSLEIVFNRGEMLSAVVDYEALNVIIRLIEQAPVQMGEESIRWAKLVIPLLVHSAQKVHLRGATALEMGMPLLLQKQQEIASITEQLMTTKLLSELQKLFMSKNETYVLKLWPLFVKLLGKTLHRSGSFINSLLQLEELGFRSGAPMIKKIAFIAWKSLIDNFALNPEILCSAKRLKLLMQPLSSIHVRTETLALTKLEVWWYLLMRLGPHLPANFEQVCVPLIQSTISIDSNASPQGSSSRVATTPGLSPMTPVHKGASPYGTPVTPRMNLNSNFGMATIPSIQLLGLEMLLHFLLGPEVVSFAKQNKLILSLEPLEHPLISSSSFFSKHSNTLITAVHDSFVAVGKDASDVVINAIWKELISLVKSVIESGNKKERPGSEVLTLLLKSLESIVNSEVFPVLKTLVLMEITIKGLPQKVLGSPAYQVANMDILNGTPALFLIQLTFNNNLLECGVEDERFFLNLETLVGCVLSGPTSPLAFSDSVLNVVNQNAKQLDNKEHLWRMWSIIVTPLTELINQTNEVNQGDALEHNFSAIYGALILPINHIFPAQKIPVVTMKTLLKTWSELYRAFARCAALVATAEENLCCEEFCSKIMSSLEDEGLSNLLFLDRITHIITVMVDCIDFSPYNIKYQPKTKSPQRPTDWSKKKKEPLGKLASLIKLIVRVIYSFHTLSLKEVHSDTLLTIGNSITSILSNLLGHISLPSMIQKIFAALTRPLALFYENSKLDEVPKVYSCMNNKLEKLLGDIIACLHVSYTGTYDSELLEQISPLLCIIFSHKNKQIRKQSAQFWNATFAKVTMLIYPEELKPILKQAKQKSLLLLPGLENVEIMEESSGPYSDMTENSQLNMKISGMERKSSGKRDSFLAQTKDTKDSMKPPAKLKLDSSTPKAKGEMPLEEEKSTDFVFIPPEVKETKERILTEHQKEVLKTKRCDIPAMYNNLDVSQDTLFSQYSQEESMEISTLIEKSKEDSKMIFKEEQKENDIVVPQHVMENCGMDEHLEKASLLNNECGSIEETSPKILSSNNDARKKALIASNKPTECASTTENPSGVSSSSVSIATISGAPLQPTSRRQSFITLEKFDGSENRPFSPSPLNNMSSTVIVKSNQEGMGKTDNPSKAKKREVALSKSDSEKITHGTKRSSRRSSKSEPIGSKKSKPLMRSEQEKSTQESVDGTVALENNPPGLLNQTECVLDNQVHLSESTVEFEDTMLESTVENTVLENNTVEERTLEVNLESKENTPPAIITDQTVNEDSHIQVTPNQKTLRRSLRRRSETAEPTTDSQDKENNHQKRERRKEEEKTPQKSPLHVKDDVLPKQKLISEQSVQGNLVDKGNNLHEKAFGETSANAEIDENRRKPDLENTKSEGDGVPDIADKSSEKPVRGRTRYQTRRASQGLLSSIENSESDSSEAKEEGSKKKRSGKWKNKSSDNVDMEDQEEKMVKQECIKIENQAHDYKGNPEVDKDIKSQICEKGDESTTVSLQDSTVSPDLLQVYDDVLNTSEGESKSNKDADHSFSNPSVPESNLRTRNASKRLHKRDSIENSMGESSKIGTSDISLLSEKTLQTVECQHKRSRRVRRSKGCDCCGEKSQSQEKSFTGLKDTENYDVKVSETKKTDGETPVSISETSKADLSSEVKLLDEHHSVNFHLDLKEENDTTNDSLIISETELKENTTQSFLPSEMVKFKETSRRDSEEAISLETQEPSNKKCKTVDPHLDVSKDISLKCFSLETKEEKSEATSKEKLSTENIVIVESNAESNPEVDAMELNVENDQSEAGFSEQKNVKTGIPGKEVTEENSERMDTPEKLKANEAVTEEFNSATDYSDTPTSVKVNAQVEISEQIAVGELGGGSDESDPGSSEEMNAEMKNCEKTAVCEVTTESDHVTETEDEDVTTTASLPIEVETKELDVEVNNFVPDTLEMITEEGKVDPNKTETNTEPNKLEETQLDDNQMVVESNETLPEVHHTSEKVEEIAQPLTSETDISGLISEDSNTSPQKSKDIDPLLMSANESPSGMHTRCVWSPLASPSTSILKRGLKRPQEDEISSPVHKVRRVSFADPIYQAGLADDIDRRCSIVRCHSSNSSLMVKSVKTSPTVQSKHNTTSAKGFLSPGSRSSKFKSSKKCLIAEMAKESVPCPTESVYPALVNCVAPVEIILPQITSNMWARGLGQLIRAKNIKTIGDLSTLTASEIKTLPIRSPKVSNVKKALRIYHEQQVKSRGLEEIPVFDISEKTINGMENKSISPDEERLASDLIDPVALEAPLSKNLVAQISALALQLDSEDLYNYSGSQLFEMHEKLGSMANSIIKNLQSRWRSPAHENSF

Target ORF information:

RefSeq Version	XM_002685359.5
Organism	Bos taurus(cattle)
Definition	Bos taurus replication timing regulatory factor 1 (RIF1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002685359.5

ORF Insert Sequence:

ATGACGGCCG CGGATCAGAG CCCCCTGGCA CCGCTGTTGG AGACTTTGGA AGACCCTTCC 
GCCTCCCTTG GAGAGCAGAC CGACGCTTAC CTGACCCTGA CCAGTCGTAT GACTGGAGAA 
GATGGAAAAG AAATCATTAC AGAAATTGAG AAAAAACTTC CTCAGCTATT TAAAGTTTTA 
AAGACTCACA TTGCCAGTCA AAACTCAGAG CTCAGTAGTG CTGCTCTACA GGCCTTGGGG 
TTTTGCTTAT ATAATCCCAA AATTGCCTCT GGATTATCAG AGATAAATAT TCAAGAACTG 
CTTTCAAAAC TGAATGATAT TGTTAAAAGT TCAGATAAAA ATGTACGTAC TAGGGCACTT 
TGGGTGATAT CCAAGCAGAC GTTTCCCACT GAAGTCGTTG GCAAAGTAGT TTCTAGTATC 
ATTGACTCAC TAGAAATAGT ATTTAATAGA GGAGAGATGC TTTCTGCTGT TGTGGATTAT 
GAAGCATTAA ATGTCATTAT AAGACTAATT GAACAAGCCC CAGTTCAAAT GGGAGAAGAG 
TCAATTAGGT GGGCAAAATT GGTCATACCT TTACTGGTTC ATTCAGCCCA AAAAGTACAT 
TTGCGGGGAG CCACTGCTCT AGAGATGGGA ATGCCTTTGT TGCTTCAGAA ACAGCAAGAA 
ATAGCATCTA TCACAGAGCA GCTTATGACT ACCAAATTAC TTTCAGAACT CCAGAAACTG 
TTTATGAGTA AAAATGAGAC TTATGTGTTA AAATTGTGGC CTTTATTTGT CAAACTTCTT 
GGGAAGACCT TGCATCGAAG TGGGAGTTTC ATTAATTCTC TGTTACAGCT GGAAGAACTG 
GGATTTCGTA GTGGAGCACC CATGATTAAA AAAATAGCTT TTATTGCTTG GAAGAGTTTA 
ATAGATAATT TTGCTTTAAA TCCAGAAATA CTGTGTAGTG CAAAAAGACT GAAGTTGTTG 
ATGCAGCCTT TGAGTTCTAT CCATGTGAGA ACAGAAACTC TAGCGCTGAC AAAACTAGAA 
GTCTGGTGGT ATTTGTTGAT GAGACTAGGA CCTCATCTTC CTGCTAATTT TGAACAGGTT 
TGTGTGCCTC TGATTCAGAG CACAATAAGC ATTGATTCTA ATGCTTCACC TCAAGGGAGT 
TCATCTCGTG TAGCTACTAC TCCAGGTTTA AGCCCTATGA CCCCAGTACA CAAAGGTGCT 
TCTCCATATG GAACTCCTGT AACCCCTCGG ATGAACTTGA ATTCAAATTT TGGAATGGCC 
ACAATCCCCT CTATACAACT TTTGGGGCTT GAAATGTTGC TTCATTTTTT GTTGGGTCCA 
GAAGTCGTGA GTTTTGCTAA GCAAAACAAA CTTATACTGA GCCTAGAACC ACTTGAACAT 
CCATTAATCA GCAGCTCTTC TTTTTTCTCC AAACACTCAA ATACACTTAT CACTGCTGTT 
CATGATAGCT TTGTTGCAGT TGGAAAAGAT GCTTCTGATG TAGTTATTAA TGCTATTTGG 
AAGGAGTTAA TTAGCTTGGT GAAGTCAGTT ATTGAATCAG GTAACAAAAA AGAGAGACCA 
GGTTCTGAAG TTTTGACTCT CTTATTAAAA TCTTTGGAAA GCATAGTGAA TTCAGAAGTA 
TTTCCTGTAT TGAAAACACT GGTTCTCATG GAAATTACAA TTAAAGGACT TCCTCAGAAA 
GTATTAGGTT CTCCAGCATA TCAGGTTGCT AATATGGATA TTCTTAATGG AACCCCTGCT 
TTGTTCTTAA TTCAGTTAAC TTTCAATAAT AATCTATTGG AATGTGGTGT GGAAGACGAA 
AGGTTTTTTC TCAACCTGGA AACACTTGTG GGCTGTGTTC TTTCGGGTCC AACTTCACCA 
CTCGCTTTCA GTGACTCTGT CTTAAATGTT GTCAATCAAA ATGCAAAGCA GTTGGATAAC 
AAGGAGCATC TTTGGAGAAT GTGGAGTATT ATAGTCACCC CATTAACAGA ATTGATTAAT 
CAGACCAATG AAGTGAATCA AGGGGATGCC TTAGAACATA ATTTCAGTGC TATCTATGGT 
GCACTGATTT TGCCAATAAA CCATATTTTT CCAGCACAAA AAATTCCAGT GGTCACCATG 
AAGACCTTAC TTAAAACTTG GTCAGAATTA TATAGAGCAT TTGCTCGCTG TGCAGCTTTG 
GTGGCAACTG CGGAAGAGAA TTTATGCTGT GAGGAATTTT GTTCCAAGAT AATGTCCAGT 
TTGGAAGATG AAGGCCTTTC AAATTTATTG TTCTTGGATA GGATCACTCA TATTATTACT 
GTAATGGTTG ATTGCATCGA CTTTTCACCA TATAATATTA AATATCAACC CAAAACTAAA 
TCACCACAGA GACCTACAGA TTGGTCCAAA AAGAAGAAGG AACCTTTAGG GAAGTTGGCT 
TCTTTAATTA AACTTATTGT GAGAGTGATC TATTCTTTTC ACACACTGAG CCTCAAGGAA 
GTACATTCTG ATACTCTCCT CACTATTGGC AACTCAATCA CCAGCATTCT TTCCAATTTA 
CTTGGACATA TTTCTCTGCC TTCTATGATC CAGAAAATAT TTGCAGCTTT AACAAGACCT 
CTGGCATTAT TTTATGAAAA CTCAAAGCTT GATGAAGTTC CTAAAGTGTA TAGTTGTATG 
AACAACAAGT TAGAAAAGCT ACTAGGAGAT ATAATTGCTT GTCTACATGT CAGCTACACT 
GGAACTTACG ACAGTGAACT TCTTGAACAG ATCTCACCAT TATTATGCAT AATATTTTCA 
CACAAGAACA AACAGATAAG AAAACAAAGT GCTCAGTTCT GGAATGCCAC ATTTGCTAAA 
GTGACGATGT TGATTTATCC TGAGGAGTTA AAGCCAATAC TGAAACAAGC CAAACAAAAA 
TCTCTGCTTT TATTGCCTGG TTTGGAAAAT GTTGAAATTA TGGAGGAATC CAGTGGACCA 
TATTCAGATA TGACAGAAAA TTCCCAATTA AATATGAAGA TAAGTGGCAT GGAAAGAAAA 
TCAAGTGGAA AAAGAGATTC TTTTTTGGCA CAAACAAAGG ATACAAAAGA CAGTATGAAA 
CCACCAGCTA AATTGAAACT AGATTCTTCA ACTCCAAAAG CAAAGGGTGA AATGCCTTTG 
GAAGAAGAAA AGTCTACTGA CTTTGTGTTT ATACCTCCAG AAGTAAAAGA AACAAAGGAA 
AGAATACTAA CTGAACACCA GAAAGAAGTA CTCAAAACAA AGAGGTGTGA TATTCCTGCC 
ATGTATAATA ATCTGGATGT TTCACAAGAT ACTTTATTTT CTCAGTATAG TCAGGAAGAA 
TCTATGGAAA TTTCTACTTT AATTGAAAAA TCAAAAGAAG ACTCCAAGAT GATATTTAAG 
GAGGAACAAA AGGAGAATGA CATTGTCGTT CCTCAACATG TCATGGAAAA CTGCGGTATG 
GATGAACATC TTGAAAAGGC TTCCCTTTTG AATAATGAGT GTGGTTCTAT TGAGGAAACC 
AGTCCAAAAA TACTGAGCAG TAATAATGAT GCCAGAAAAA AAGCTTTAAT TGCTTCAAAT 
AAACCAACAG AATGTGCATC TACTACAGAA AATCCATCTG GTGTCAGCAG TAGCTCGGTT 
TCTATTGCCA CCATTTCTGG AGCTCCCTTA CAGCCTACAA GTCGAAGGCA ATCCTTTATT 
ACTTTGGAAA AGTTTGATGG TTCAGAAAAT AGACCTTTTA GTCCATCACC CTTGAATAAT 
ATGTCATCAA CTGTAATAGT GAAAAGTAAC CAGGAAGGCA TGGGTAAAAC AGATAATCCA 
TCAAAAGCAA AGAAGAGAGA AGTGGCTCTT TCCAAATCTG ACTCTGAAAA AATAACACAT 
GGGACTAAGA GATCAAGCCG AAGGTCGAGT AAATCTGAAC CAATAGGGAG TAAAAAGTCT 
AAACCCTTAA TGAGATCTGA ACAAGAGAAA AGTACTCAAG AATCTGTTGA TGGCACAGTA 
GCCTTAGAAA ATAACCCACC TGGCTTGCTT AATCAGACAG AATGTGTGTT AGATAATCAG 
GTTCATCTCT CTGAATCTAC AGTGGAGTTT GAGGATACAA TGCTTGAATC AACAGTGGAG 
AATACTGTAT TAGAAAACAA TACTGTAGAA GAGAGAACCT TAGAAGTTAA CTTGGAATCC 
AAAGAAAACA CACCCCCAGC CATAATAACG GATCAGACAG TAAATGAGGA TAGTCATATT 
CAGGTAACTC CAAATCAGAA AACCCTTAGG CGATCTTTAA GGCGACGTTC AGAAACAGCA 
GAACCAACCA CTGATAGCCA AGATAAAGAA AATAATCATC AAAAAAGGGA AAGACGTAAA 
GAAGAAGAAA AGACTCCGCA GAAGAGTCCG TTACATGTAA AAGATGATGT TTTACCTAAG 
CAAAAATTGA TTTCTGAACA AAGTGTACAG GGAAACTTAG TTGACAAAGG AAATAATTTA 
CATGAGAAGG CTTTTGGGGA GACCAGTGCT AATGCTGAAA TTGATGAAAA TAGAAGAAAG 
CCAGATCTTG AGAATACTAA ATCTGAAGGA GATGGTGTGC CGGACATTGC AGATAAATCC 
TCTGAGAAAC CAGTAAGGGG ACGAACACGA TACCAAACAA GAAGAGCATC CCAGGGTTTA 
CTTTCCAGCA TTGAAAACTC AGAATCTGAT AGTTCTGAGG CAAAAGAAGA AGGTTCTAAA 
AAGAAAAGAT CTGGAAAATG GAAGAACAAA AGCAGTGACA ATGTTGACAT GGAAGATCAA 
GAAGAGAAAA TGGTAAAACA GGAATGTATA AAAATTGAAA ATCAGGCACA TGATTATAAA 
GGGAATCCTG AAGTAGACAA AGATATAAAA TCTCAAATCT GTGAAAAAGG AGATGAAAGT 
ACTACTGTAA GTCTTCAAGA TTCTACCGTA TCTCCAGATT TATTACAAGT TTATGATGAT 
GTATTGAATA CTTCTGAGGG AGAAAGTAAA AGTAACAAAG ATGCAGACCA TTCCTTTTCA 
AATCCTTCTG TACCAGAATC AAATCTAAGG ACTAGAAATG CCAGTAAGAG GTTACATAAG 
CGAGATTCTA TAGAAAATAG CATGGGGGAA TCCTCAAAAA TAGGGACATC AGACATTTCT 
TTGCTTTCTG AAAAAACTCT TCAGACAGTA GAATGCCAGC ACAAGAGAAG TAGGAGGGTG 
AGGCGATCTA AAGGTTGTGA TTGTTGTGGA GAAAAATCAC AGTCTCAGGA AAAGTCATTT 
ACTGGGTTAA AGGATACAGA AAATTACGAT GTAAAAGTCA GTGAAACAAA AAAGACAGAT 
GGGGAAACAC CTGTATCTAT TTCAGAAACT TCGAAAGCTG ATCTGTCCTC GGAGGTTAAA 
CTTTTAGATG AGCATCATAG TGTGAATTTT CATTTGGATC TCAAGGAAGA GAATGATACT 
ACTAATGATT CATTAATTAT ATCTGAAACT GAGTTAAAAG AAAATACTAC TCAAAGCTTT 
CTTCCTTCTG AAATGGTAAA ATTTAAAGAA ACTTCTCGTA GGGATTCTGA GGAAGCAATA 
TCTCTAGAAA CCCAGGAGCC ATCTAATAAG AAATGTAAAA CTGTTGACCC ACATTTAGAT 
GTTTCCAAAG ATATTTCACT GAAATGTTTT TCTTTGGAGA CTAAAGAAGA AAAGTCAGAG 
GCCACTTCAA AAGAGAAATT GAGTACAGAG AACATTGTTA TTGTTGAATC AAATGCAGAA 
TCAAATCCAG AAGTAGATGC TATGGAATTG AATGTAGAAA ATGATCAGTC TGAAGCTGGC 
TTCTCTGAAC AAAAGAATGT CAAAACTGGT ATTCCAGGGA AAGAAGTAAC AGAGGAAAAC 
AGTGAAAGAA TGGATACACC TGAAAAACTG AAAGCCAATG AAGCAGTAAC TGAGGAATTT 
AATTCAGCTA CTGATTATTC GGATACTCCG ACATCTGTAA AAGTGAATGC TCAGGTAGAG 
ATCTCTGAAC AAATAGCGGT TGGGGAGCTA GGTGGAGGAA GTGATGAATC TGATCCAGGC 
TCATCTGAAG AAATGAACGC TGAAATGAAA AATTGTGAAA AAACAGCGGT TTGTGAGGTG 
ACTACTGAAT CTGACCACGT CACTGAAACC GAAGACGAAG ATGTAACTAC CACGGCTTCT 
CTGCCTATAG AAGTTGAAAC AAAGGAATTA GATGTAGAAG TCAATAACTT TGTTCCCGAC 
ACACTTGAGA TGATCACTGA AGAAGGAAAG GTTGACCCTA ACAAAACTGA AACGAATACT 
GAACCTAATA AATTGGAGGA AACACAGTTA GATGACAATC AGATGGTAGT GGAAAGTAAT 
GAAACTTTAC CGGAAGTTCA CCATACTTCA GAGAAAGTAG AGGAGATAGC ACAACCTCTG 
ACATCTGAAA CAGATATTTC TGGACTAATA TCAGAAGACA GTAATACATC TCCTCAAAAA 
TCGAAGGATA TTGATCCTTT ACTCATGTCA GCAAACGAGA GTCCTAGTGG CATGCACACA 
CGTTGTGTGT GGTCTCCTTT GGCTTCTCCA TCCACCAGCA TTTTAAAGAG AGGACTAAAA 
AGACCCCAAG AAGATGAAAT CTCGTCACCT GTCCACAAGG TTCGCCGTGT CTCCTTTGCA 
GATCCTATAT ACCAAGCAGG ATTGGCAGAT GACATTGATA GGCGATGCTC TATTGTTAGG 
TGCCATTCTT CAAATAGTTC TCTCATGGTA AAAAGTGTTA AAACTTCACC TACTGTGCAA 
TCTAAGCATA ATACCACTTC AGCCAAAGGA TTTCTGTCCC CAGGATCACG TAGCTCTAAA 
TTTAAGAGCT CAAAGAAGTG TTTAATTGCA GAAATGGCTA AGGAATCTGT GCCATGCCCA 
ACAGAAAGTG TTTACCCTGC TTTGGTGAAC TGTGTAGCAC CAGTTGAAAT CATTTTACCT 
CAGATTACAT CCAACATGTG GGCAAGAGGA CTGGGACAGC TCATTAGAGC CAAGAATATA 
AAAACAATTG GCGATTTGAG TACCCTTACA GCATCTGAAA TAAAAACTCT TCCTATCCGT 
TCTCCAAAAG TGTCTAACGT TAAAAAGGCT CTCAGAATAT ATCATGAGCA GCAGGTGAAG 
TCTCGTGGAC TAGAAGAGAT TCCAGTTTTT GATATTTCTG AAAAAACAAT AAATGGAATG 
GAAAATAAAT CTATCTCTCC TGATGAAGAA AGACTTGCCT CAGATTTAAT TGATCCTGTT 
GCTTTAGAAG CTCCGTTATC TAAAAATCTT GTGGCACAGA TTAGCGCACT TGCTCTTCAA 
CTAGATTCAG AAGATCTCTA TAATTATTCA GGAAGCCAGC TATTTGAAAT GCACGAGAAA 
CTTGGTAGCA TGGCAAACTC TATAATTAAA AATCTGCAGT CACGTTGGAG GTCACCAGCC 
CATGAAAATT CTTTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RIF1 ( XM_024980692.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024836460.1 Bos taurus replication timing regulatory factor 1 (RIF1), transcript variant X5, mRNA.

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CloneID	OBa02875
Clone ID Related Accession (Same CDS sequence)	XM_010826492.2 , XM_002685359.6 , XM_002685359.5 , XM_024980692.1 , XM_024980695.1 , XM_010802076.2 , XM_615269.9
Accession Version	XM_024980692.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7398bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	telomere-associated protein RIF1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGCCG CGGATCAGAG CCCCCTGGCA CCGCTGTTGG AGACTTTGGA AGACCCTTCC 
GCCTCCCTTG GAGAGCAGAC CGACGCTTAC CTGACCCTGA CCAGTCGTAT GACTGGAGAA 
GATGGAAAAG AAATCATTAC AGAAATTGAG AAAAAACTTC CTCAGCTATT TAAAGTTTTA 
AAGACTCACA TTGCCAGTCA AAACTCAGAG CTCAGTAGTG CTGCTCTACA GGCCTTGGGG 
TTTTGCTTAT ATAATCCCAA AATTGCCTCT GGATTATCAG AGATAAATAT TCAAGAACTG 
CTTTCAAAAC TGAATGATAT TGTTAAAAGT TCAGATAAAA ATGTACGTAC TAGGGCACTT 
TGGGTGATAT CCAAGCAGAC GTTTCCCACT GAAGTCGTTG GCAAAGTAGT TTCTAGTATC 
ATTGACTCAC TAGAAATAGT ATTTAATAGA GGAGAGATGC TTTCTGCTGT TGTGGATTAT 
GAAGCATTAA ATGTCATTAT AAGACTAATT GAACAAGCCC CAGTTCAAAT GGGAGAAGAG 
TCAATTAGGT GGGCAAAATT GGTCATACCT TTACTGGTTC ATTCAGCCCA AAAAGTACAT 
TTGCGGGGAG CCACTGCTCT AGAGATGGGA ATGCCTTTGT TGCTTCAGAA ACAGCAAGAA 
ATAGCATCTA TCACAGAGCA GCTTATGACT ACCAAATTAC TTTCAGAACT CCAGAAACTG 
TTTATGAGTA AAAATGAGAC TTATGTGTTA AAATTGTGGC CTTTATTTGT CAAACTTCTT 
GGGAAGACCT TGCATCGAAG TGGGAGTTTC ATTAATTCTC TGTTACAGCT GGAAGAACTG 
GGATTTCGTA GTGGAGCACC CATGATTAAA AAAATAGCTT TTATTGCTTG GAAGAGTTTA 
ATAGATAATT TTGCTTTAAA TCCAGAAATA CTGTGTAGTG CAAAAAGACT GAAGTTGTTG 
ATGCAGCCTT TGAGTTCTAT CCATGTGAGA ACAGAAACTC TAGCGCTGAC AAAACTAGAA 
GTCTGGTGGT ATTTGTTGAT GAGACTAGGA CCTCATCTTC CTGCTAATTT TGAACAGGTT 
TGTGTGCCTC TGATTCAGAG CACAATAAGC ATTGATTCTA ATGCTTCACC TCAAGGGAGT 
TCATCTCGTG TAGCTACTAC TCCAGGTTTA AGCCCTATGA CCCCAGTACA CAAAGGTGCT 
TCTCCATATG GAACTCCTGT AACCCCTCGG ATGAACTTGA ATTCAAATTT TGGAATGGCC 
ACAATCCCCT CTATACAACT TTTGGGGCTT GAAATGTTGC TTCATTTTTT GTTGGGTCCA 
GAAGTCGTGA GTTTTGCTAA GCAAAACAAA CTTATACTGA GCCTAGAACC ACTTGAACAT 
CCATTAATCA GCAGCTCTTC TTTTTTCTCC AAACACTCAA ATACACTTAT CACTGCTGTT 
CATGATAGCT TTGTTGCAGT TGGAAAAGAT GCTTCTGATG TAGTTATTAA TGCTATTTGG 
AAGGAGTTAA TTAGCTTGGT GAAGTCAGTT ATTGAATCAG GTAACAAAAA AGAGAGACCA 
GGTTCTGAAG TTTTGACTCT CTTATTAAAA TCTTTGGAAA GCATAGTGAA TTCAGAAGTA 
TTTCCTGTAT TGAAAACACT GGTTCTCATG GAAATTACAA TTAAAGGACT TCCTCAGAAA 
GTATTAGGTT CTCCAGCATA TCAGGTTGCT AATATGGATA TTCTTAATGG AACCCCTGCT 
TTGTTCTTAA TTCAGTTAAC TTTCAATAAT AATCTATTGG AATGTGGTGT GGAAGACGAA 
AGGTTTTTTC TCAACCTGGA AACACTTGTG GGCTGTGTTC TTTCGGGTCC AACTTCACCA 
CTCGCTTTCA GTGACTCTGT CTTAAATGTT GTCAATCAAA ATGCAAAGCA GTTGGATAAC 
AAGGAGCATC TTTGGAGAAT GTGGAGTATT ATAGTCACCC CATTAACAGA ATTGATTAAT 
CAGACCAATG AAGTGAATCA AGGGGATGCC TTAGAACATA ATTTCAGTGC TATCTATGGT 
GCACTGATTT TGCCAATAAA CCATATTTTT CCAGCACAAA AAATTCCAGT GGTCACCATG 
AAGACCTTAC TTAAAACTTG GTCAGAATTA TATAGAGCAT TTGCTCGCTG TGCAGCTTTG 
GTGGCAACTG CGGAAGAGAA TTTATGCTGT GAGGAATTTT GTTCCAAGAT AATGTCCAGT 
TTGGAAGATG AAGGCCTTTC AAATTTATTG TTCTTGGATA GGATCACTCA TATTATTACT 
GTAATGGTTG ATTGCATCGA CTTTTCACCA TATAATATTA AATATCAACC CAAAACTAAA 
TCACCACAGA GACCTACAGA TTGGTCCAAA AAGAAGAAGG AACCTTTAGG GAAGTTGGCT 
TCTTTAATTA AACTTATTGT GAGAGTGATC TATTCTTTTC ACACACTGAG CCTCAAGGAA 
GTACATTCTG ATACTCTCCT CACTATTGGC AACTCAATCA CCAGCATTCT TTCCAATTTA 
CTTGGACATA TTTCTCTGCC TTCTATGATC CAGAAAATAT TTGCAGCTTT AACAAGACCT 
CTGGCATTAT TTTATGAAAA CTCAAAGCTT GATGAAGTTC CTAAAGTGTA TAGTTGTATG 
AACAACAAGT TAGAAAAGCT ACTAGGAGAT ATAATTGCTT GTCTACATGT CAGCTACACT 
GGAACTTACG ACAGTGAACT TCTTGAACAG ATCTCACCAT TATTATGCAT AATATTTTCA 
CACAAGAACA AACAGATAAG AAAACAAAGT GCTCAGTTCT GGAATGCCAC ATTTGCTAAA 
GTGACGATGT TGATTTATCC TGAGGAGTTA AAGCCAATAC TGAAACAAGC CAAACAAAAA 
TCTCTGCTTT TATTGCCTGG TTTGGAAAAT GTTGAAATTA TGGAGGAATC CAGTGGACCA 
TATTCAGATA TGACAGAAAA TTCCCAATTA AATATGAAGA TAAGTGGCAT GGAAAGAAAA 
TCAAGTGGAA AAAGAGATTC TTTTTTGGCA CAAACAAAGG ATACAAAAGA CAGTATGAAA 
CCACCAGCTA AATTGAAACT AGATTCTTCA ACTCCAAAAG CAAAGGGTGA AATGCCTTTG 
GAAGAAGAAA AGTCTACTGA CTTTGTGTTT ATACCTCCAG AAGTAAAAGA AACAAAGGAA 
AGAATACTAA CTGAACACCA GAAAGAAGTA CTCAAAACAA AGAGGTGTGA TATTCCTGCC 
ATGTATAATA ATCTGGATGT TTCACAAGAT ACTTTATTTT CTCAGTATAG TCAGGAAGAA 
TCTATGGAAA TTTCTACTTT AATTGAAAAA TCAAAAGAAG ACTCCAAGAT GATATTTAAG 
GAGGAACAAA AGGAGAATGA CATTGTCGTT CCTCAACATG TCATGGAAAA CTGCGGTATG 
GATGAACATC TTGAAAAGGC TTCCCTTTTG AATAATGAGT GTGGTTCTAT TGAGGAAACC 
AGTCCAAAAA TACTGAGCAG TAATAATGAT GCCAGAAAAA AAGCTTTAAT TGCTTCAAAT 
AAACCAACAG AATGTGCATC TACTACAGAA AATCCATCTG GTGTCAGCAG TAGCTCGGTT 
TCTATTGCCA CCATTTCTGG AGCTCCCTTA CAGCCTACAA GTCGAAGGCA ATCCTTTATT 
ACTTTGGAAA AGTTTGATGG TTCAGAAAAT AGACCTTTTA GTCCATCACC CTTGAATAAT 
ATGTCATCAA CTGTAATAGT GAAAAGTAAC CAGGAAGGCA TGGGTAAAAC AGATAATCCA 
TCAAAAGCAA AGAAGAGAGA AGTGGCTCTT TCCAAATCTG ACTCTGAAAA AATAACACAT 
GGGACTAAGA GATCAAGCCG AAGGTCGAGT AAATCTGAAC CAATAGGGAG TAAAAAGTCT 
AAACCCTTAA TGAGATCTGA ACAAGAGAAA AGTACTCAAG AATCTGTTGA TGGCACAGTA 
GCCTTAGAAA ATAACCCACC TGGCTTGCTT AATCAGACAG AATGTGTGTT AGATAATCAG 
GTTCATCTCT CTGAATCTAC AGTGGAGTTT GAGGATACAA TGCTTGAATC AACAGTGGAG 
AATACTGTAT TAGAAAACAA TACTGTAGAA GAGAGAACCT TAGAAGTTAA CTTGGAATCC 
AAAGAAAACA CACCCCCAGC CATAATAACG GATCAGACAG TAAATGAGGA TAGTCATATT 
CAGGTAACTC CAAATCAGAA AACCCTTAGG CGATCTTTAA GGCGACGTTC AGAAACAGCA 
GAACCAACCA CTGATAGCCA AGATAAAGAA AATAATCATC AAAAAAGGGA AAGACGTAAA 
GAAGAAGAAA AGACTCCGCA GAAGAGTCCG TTACATGTAA AAGATGATGT TTTACCTAAG 
CAAAAATTGA TTTCTGAACA AAGTGTACAG GGAAACTTAG TTGACAAAGG AAATAATTTA 
CATGAGAAGG CTTTTGGGGA GACCAGTGCT AATGCTGAAA TTGATGAAAA TAGAAGAAAG 
CCAGATCTTG AGAATACTAA ATCTGAAGGA GATGGTGTGC CGGACATTGC AGATAAATCC 
TCTGAGAAAC CAGTAAGGGG ACGAACACGA TACCAAACAA GAAGAGCATC CCAGGGTTTA 
CTTTCCAGCA TTGAAAACTC AGAATCTGAT AGTTCTGAGG CAAAAGAAGA AGGTTCTAAA 
AAGAAAAGAT CTGGAAAATG GAAGAACAAA AGCAGTGACA ATGTTGACAT GGAAGATCAA 
GAAGAGAAAA TGGTAAAACA GGAATGTATA AAAATTGAAA ATCAGGCACA TGATTATAAA 
GGGAATCCTG AAGTAGACAA AGATATAAAA TCTCAAATCT GTGAAAAAGG AGATGAAAGT 
ACTACTGTAA GTCTTCAAGA TTCTACCGTA TCTCCAGATT TATTACAAGT TTATGATGAT 
GTATTGAATA CTTCTGAGGG AGAAAGTAAA AGTAACAAAG ATGCAGACCA TTCCTTTTCA 
AATCCTTCTG TACCAGAATC AAATCTAAGG ACTAGAAATG CCAGTAAGAG GTTACATAAG 
CGAGATTCTA TAGAAAATAG CATGGGGGAA TCCTCAAAAA TAGGGACATC AGACATTTCT 
TTGCTTTCTG AAAAAACTCT TCAGACAGTA GAATGCCAGC ACAAGAGAAG TAGGAGGGTG 
AGGCGATCTA AAGGTTGTGA TTGTTGTGGA GAAAAATCAC AGTCTCAGGA AAAGTCATTT 
ACTGGGTTAA AGGATACAGA AAATTACGAT GTAAAAGTCA GTGAAACAAA AAAGACAGAT 
GGGGAAACAC CTGTATCTAT TTCAGAAACT TCGAAAGCTG ATCTGTCCTC GGAGGTTAAA 
CTTTTAGATG AGCATCATAG TGTGAATTTT CATTTGGATC TCAAGGAAGA GAATGATACT 
ACTAATGATT CATTAATTAT ATCTGAAACT GAGTTAAAAG AAAATACTAC TCAAAGCTTT 
CTTCCTTCTG AAATGGTAAA ATTTAAAGAA ACTTCTCGTA GGGATTCTGA GGAAGCAATA 
TCTCTAGAAA CCCAGGAGCC ATCTAATAAG AAATGTAAAA CTGTTGACCC ACATTTAGAT 
GTTTCCAAAG ATATTTCACT GAAATGTTTT TCTTTGGAGA CTAAAGAAGA AAAGTCAGAG 
GCCACTTCAA AAGAGAAATT GAGTACAGAG AACATTGTTA TTGTTGAATC AAATGCAGAA 
TCAAATCCAG AAGTAGATGC TATGGAATTG AATGTAGAAA ATGATCAGTC TGAAGCTGGC 
TTCTCTGAAC AAAAGAATGT CAAAACTGGT ATTCCAGGGA AAGAAGTAAC AGAGGAAAAC 
AGTGAAAGAA TGGATACACC TGAAAAACTG AAAGCCAATG AAGCAGTAAC TGAGGAATTT 
AATTCAGCTA CTGATTATTC GGATACTCCG ACATCTGTAA AAGTGAATGC TCAGGTAGAG 
ATCTCTGAAC AAATAGCGGT TGGGGAGCTA GGTGGAGGAA GTGATGAATC TGATCCAGGC 
TCATCTGAAG AAATGAACGC TGAAATGAAA AATTGTGAAA AAACAGCGGT TTGTGAGGTG 
ACTACTGAAT CTGACCACGT CACTGAAACC GAAGACGAAG ATGTAACTAC CACGGCTTCT 
CTGCCTATAG AAGTTGAAAC AAAGGAATTA GATGTAGAAG TCAATAACTT TGTTCCCGAC 
ACACTTGAGA TGATCACTGA AGAAGGAAAG GTTGACCCTA ACAAAACTGA AACGAATACT 
GAACCTAATA AATTGGAGGA AACACAGTTA GATGACAATC AGATGGTAGT GGAAAGTAAT 
GAAACTTTAC CGGAAGTTCA CCATACTTCA GAGAAAGTAG AGGAGATAGC ACAACCTCTG 
ACATCTGAAA CAGATATTTC TGGACTAATA TCAGAAGACA GTAATACATC TCCTCAAAAA 
TCGAAGGATA TTGATCCTTT ACTCATGTCA GCAAACGAGA GTCCTAGTGG CATGCACACA 
CGTTGTGTGT GGTCTCCTTT GGCTTCTCCA TCCACCAGCA TTTTAAAGAG AGGACTAAAA 
AGACCCCAAG AAGATGAAAT CTCGTCACCT GTCCACAAGG TTCGCCGTGT CTCCTTTGCA 
GATCCTATAT ACCAAGCAGG ATTGGCAGAT GACATTGATA GGCGATGCTC TATTGTTAGG 
TGCCATTCTT CAAATAGTTC TCTCATGGTA AAAAGTGTTA AAACTTCACC TACTGTGCAA 
TCTAAGCATA ATACCACTTC AGCCAAAGGA TTTCTGTCCC CAGGATCACG TAGCTCTAAA 
TTTAAGAGCT CAAAGAAGTG TTTAATTGCA GAAATGGCTA AGGAATCTGT GCCATGCCCA 
ACAGAAAGTG TTTACCCTGC TTTGGTGAAC TGTGTAGCAC CAGTTGAAAT CATTTTACCT 
CAGATTACAT CCAACATGTG GGCAAGAGGA CTGGGACAGC TCATTAGAGC CAAGAATATA 
AAAACAATTG GCGATTTGAG TACCCTTACA GCATCTGAAA TAAAAACTCT TCCTATCCGT 
TCTCCAAAAG TGTCTAACGT TAAAAAGGCT CTCAGAATAT ATCATGAGCA GCAGGTGAAG 
TCTCGTGGAC TAGAAGAGAT TCCAGTTTTT GATATTTCTG AAAAAACAAT AAATGGAATG 
GAAAATAAAT CTATCTCTCC TGATGAAGAA AGACTTGCCT CAGATTTAAT TGATCCTGTT 
GCTTTAGAAG CTCCGTTATC TAAAAATCTT GTGGCACAGA TTAGCGCACT TGCTCTTCAA 
CTAGATTCAG AAGATCTCTA TAATTATTCA GGAAGCCAGC TATTTGAAAT GCACGAGAAA 
CTTGGTAGCA TGGCAAACTC TATAATTAAA AATCTGCAGT CACGTTGGAG GTCACCAGCC 
CATGAAAATT CTTTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024836460.1
CDS	185..7582
Translation	MTAADQSPLAPLLETLEDPSASLGEQTDAYLTLTSRMTGEDGKEIITEIEKKLPQLFKVLKTHIASQNSELSSAALQALGFCLYNPKIASGLSEINIQELLSKLNDIVKSSDKNVRTRALWVISKQTFPTEVVGKVVSSIIDSLEIVFNRGEMLSAVVDYEALNVIIRLIEQAPVQMGEESIRWAKLVIPLLVHSAQKVHLRGATALEMGMPLLLQKQQEIASITEQLMTTKLLSELQKLFMSKNETYVLKLWPLFVKLLGKTLHRSGSFINSLLQLEELGFRSGAPMIKKIAFIAWKSLIDNFALNPEILCSAKRLKLLMQPLSSIHVRTETLALTKLEVWWYLLMRLGPHLPANFEQVCVPLIQSTISIDSNASPQGSSSRVATTPGLSPMTPVHKGASPYGTPVTPRMNLNSNFGMATIPSIQLLGLEMLLHFLLGPEVVSFAKQNKLILSLEPLEHPLISSSSFFSKHSNTLITAVHDSFVAVGKDASDVVINAIWKELISLVKSVIESGNKKERPGSEVLTLLLKSLESIVNSEVFPVLKTLVLMEITIKGLPQKVLGSPAYQVANMDILNGTPALFLIQLTFNNNLLECGVEDERFFLNLETLVGCVLSGPTSPLAFSDSVLNVVNQNAKQLDNKEHLWRMWSIIVTPLTELINQTNEVNQGDALEHNFSAIYGALILPINHIFPAQKIPVVTMKTLLKTWSELYRAFARCAALVATAEENLCCEEFCSKIMSSLEDEGLSNLLFLDRITHIITVMVDCIDFSPYNIKYQPKTKSPQRPTDWSKKKKEPLGKLASLIKLIVRVIYSFHTLSLKEVHSDTLLTIGNSITSILSNLLGHISLPSMIQKIFAALTRPLALFYENSKLDEVPKVYSCMNNKLEKLLGDIIACLHVSYTGTYDSELLEQISPLLCIIFSHKNKQIRKQSAQFWNATFAKVTMLIYPEELKPILKQAKQKSLLLLPGLENVEIMEESSGPYSDMTENSQLNMKISGMERKSSGKRDSFLAQTKDTKDSMKPPAKLKLDSSTPKAKGEMPLEEEKSTDFVFIPPEVKETKERILTEHQKEVLKTKRCDIPAMYNNLDVSQDTLFSQYSQEESMEISTLIEKSKEDSKMIFKEEQKENDIVVPQHVMENCGMDEHLEKASLLNNECGSIEETSPKILSSNNDARKKALIASNKPTECASTTENPSGVSSSSVSIATISGAPLQPTSRRQSFITLEKFDGSENRPFSPSPLNNMSSTVIVKSNQEGMGKTDNPSKAKKREVALSKSDSEKITHGTKRSSRRSSKSEPIGSKKSKPLMRSEQEKSTQESVDGTVALENNPPGLLNQTECVLDNQVHLSESTVEFEDTMLESTVENTVLENNTVEERTLEVNLESKENTPPAIITDQTVNEDSHIQVTPNQKTLRRSLRRRSETAEPTTDSQDKENNHQKRERRKEEEKTPQKSPLHVKDDVLPKQKLISEQSVQGNLVDKGNNLHEKAFGETSANAEIDENRRKPDLENTKSEGDGVPDIADKSSEKPVRGRTRYQTRRASQGLLSSIENSESDSSEAKEEGSKKKRSGKWKNKSSDNVDMEDQEEKMVKQECIKIENQAHDYKGNPEVDKDIKSQICEKGDESTTVSLQDSTVSPDLLQVYDDVLNTSEGESKSNKDADHSFSNPSVPESNLRTRNASKRLHKRDSIENSMGESSKIGTSDISLLSEKTLQTVECQHKRSRRVRRSKGCDCCGEKSQSQEKSFTGLKDTENYDVKVSETKKTDGETPVSISETSKADLSSEVKLLDEHHSVNFHLDLKEENDTTNDSLIISETELKENTTQSFLPSEMVKFKETSRRDSEEAISLETQEPSNKKCKTVDPHLDVSKDISLKCFSLETKEEKSEATSKEKLSTENIVIVESNAESNPEVDAMELNVENDQSEAGFSEQKNVKTGIPGKEVTEENSERMDTPEKLKANEAVTEEFNSATDYSDTPTSVKVNAQVEISEQIAVGELGGGSDESDPGSSEEMNAEMKNCEKTAVCEVTTESDHVTETEDEDVTTTASLPIEVETKELDVEVNNFVPDTLEMITEEGKVDPNKTETNTEPNKLEETQLDDNQMVVESNETLPEVHHTSEKVEEIAQPLTSETDISGLISEDSNTSPQKSKDIDPLLMSANESPSGMHTRCVWSPLASPSTSILKRGLKRPQEDEISSPVHKVRRVSFADPIYQAGLADDIDRRCSIVRCHSSNSSLMVKSVKTSPTVQSKHNTTSAKGFLSPGSRSSKFKSSKKCLIAEMAKESVPCPTESVYPALVNCVAPVEIILPQITSNMWARGLGQLIRAKNIKTIGDLSTLTASEIKTLPIRSPKVSNVKKALRIYHEQQVKSRGLEEIPVFDISEKTINGMENKSISPDEERLASDLIDPVALEAPLSKNLVAQISALALQLDSEDLYNYSGSQLFEMHEKLGSMANSIIKNLQSRWRSPAHENSF

Target ORF information:

RefSeq Version	XM_024980692.1
Organism	Bos taurus(cattle)
Definition	Bos taurus replication timing regulatory factor 1 (RIF1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024980692.1

ORF Insert Sequence:

ATGACGGCCG CGGATCAGAG CCCCCTGGCA CCGCTGTTGG AGACTTTGGA AGACCCTTCC 
GCCTCCCTTG GAGAGCAGAC CGACGCTTAC CTGACCCTGA CCAGTCGTAT GACTGGAGAA 
GATGGAAAAG AAATCATTAC AGAAATTGAG AAAAAACTTC CTCAGCTATT TAAAGTTTTA 
AAGACTCACA TTGCCAGTCA AAACTCAGAG CTCAGTAGTG CTGCTCTACA GGCCTTGGGG 
TTTTGCTTAT ATAATCCCAA AATTGCCTCT GGATTATCAG AGATAAATAT TCAAGAACTG 
CTTTCAAAAC TGAATGATAT TGTTAAAAGT TCAGATAAAA ATGTACGTAC TAGGGCACTT 
TGGGTGATAT CCAAGCAGAC GTTTCCCACT GAAGTCGTTG GCAAAGTAGT TTCTAGTATC 
ATTGACTCAC TAGAAATAGT ATTTAATAGA GGAGAGATGC TTTCTGCTGT TGTGGATTAT 
GAAGCATTAA ATGTCATTAT AAGACTAATT GAACAAGCCC CAGTTCAAAT GGGAGAAGAG 
TCAATTAGGT GGGCAAAATT GGTCATACCT TTACTGGTTC ATTCAGCCCA AAAAGTACAT 
TTGCGGGGAG CCACTGCTCT AGAGATGGGA ATGCCTTTGT TGCTTCAGAA ACAGCAAGAA 
ATAGCATCTA TCACAGAGCA GCTTATGACT ACCAAATTAC TTTCAGAACT CCAGAAACTG 
TTTATGAGTA AAAATGAGAC TTATGTGTTA AAATTGTGGC CTTTATTTGT CAAACTTCTT 
GGGAAGACCT TGCATCGAAG TGGGAGTTTC ATTAATTCTC TGTTACAGCT GGAAGAACTG 
GGATTTCGTA GTGGAGCACC CATGATTAAA AAAATAGCTT TTATTGCTTG GAAGAGTTTA 
ATAGATAATT TTGCTTTAAA TCCAGAAATA CTGTGTAGTG CAAAAAGACT GAAGTTGTTG 
ATGCAGCCTT TGAGTTCTAT CCATGTGAGA ACAGAAACTC TAGCGCTGAC AAAACTAGAA 
GTCTGGTGGT ATTTGTTGAT GAGACTAGGA CCTCATCTTC CTGCTAATTT TGAACAGGTT 
TGTGTGCCTC TGATTCAGAG CACAATAAGC ATTGATTCTA ATGCTTCACC TCAAGGGAGT 
TCATCTCGTG TAGCTACTAC TCCAGGTTTA AGCCCTATGA CCCCAGTACA CAAAGGTGCT 
TCTCCATATG GAACTCCTGT AACCCCTCGG ATGAACTTGA ATTCAAATTT TGGAATGGCC 
ACAATCCCCT CTATACAACT TTTGGGGCTT GAAATGTTGC TTCATTTTTT GTTGGGTCCA 
GAAGTCGTGA GTTTTGCTAA GCAAAACAAA CTTATACTGA GCCTAGAACC ACTTGAACAT 
CCATTAATCA GCAGCTCTTC TTTTTTCTCC AAACACTCAA ATACACTTAT CACTGCTGTT 
CATGATAGCT TTGTTGCAGT TGGAAAAGAT GCTTCTGATG TAGTTATTAA TGCTATTTGG 
AAGGAGTTAA TTAGCTTGGT GAAGTCAGTT ATTGAATCAG GTAACAAAAA AGAGAGACCA 
GGTTCTGAAG TTTTGACTCT CTTATTAAAA TCTTTGGAAA GCATAGTGAA TTCAGAAGTA 
TTTCCTGTAT TGAAAACACT GGTTCTCATG GAAATTACAA TTAAAGGACT TCCTCAGAAA 
GTATTAGGTT CTCCAGCATA TCAGGTTGCT AATATGGATA TTCTTAATGG AACCCCTGCT 
TTGTTCTTAA TTCAGTTAAC TTTCAATAAT AATCTATTGG AATGTGGTGT GGAAGACGAA 
AGGTTTTTTC TCAACCTGGA AACACTTGTG GGCTGTGTTC TTTCGGGTCC AACTTCACCA 
CTCGCTTTCA GTGACTCTGT CTTAAATGTT GTCAATCAAA ATGCAAAGCA GTTGGATAAC 
AAGGAGCATC TTTGGAGAAT GTGGAGTATT ATAGTCACCC CATTAACAGA ATTGATTAAT 
CAGACCAATG AAGTGAATCA AGGGGATGCC TTAGAACATA ATTTCAGTGC TATCTATGGT 
GCACTGATTT TGCCAATAAA CCATATTTTT CCAGCACAAA AAATTCCAGT GGTCACCATG 
AAGACCTTAC TTAAAACTTG GTCAGAATTA TATAGAGCAT TTGCTCGCTG TGCAGCTTTG 
GTGGCAACTG CGGAAGAGAA TTTATGCTGT GAGGAATTTT GTTCCAAGAT AATGTCCAGT 
TTGGAAGATG AAGGCCTTTC AAATTTATTG TTCTTGGATA GGATCACTCA TATTATTACT 
GTAATGGTTG ATTGCATCGA CTTTTCACCA TATAATATTA AATATCAACC CAAAACTAAA 
TCACCACAGA GACCTACAGA TTGGTCCAAA AAGAAGAAGG AACCTTTAGG GAAGTTGGCT 
TCTTTAATTA AACTTATTGT GAGAGTGATC TATTCTTTTC ACACACTGAG CCTCAAGGAA 
GTACATTCTG ATACTCTCCT CACTATTGGC AACTCAATCA CCAGCATTCT TTCCAATTTA 
CTTGGACATA TTTCTCTGCC TTCTATGATC CAGAAAATAT TTGCAGCTTT AACAAGACCT 
CTGGCATTAT TTTATGAAAA CTCAAAGCTT GATGAAGTTC CTAAAGTGTA TAGTTGTATG 
AACAACAAGT TAGAAAAGCT ACTAGGAGAT ATAATTGCTT GTCTACATGT CAGCTACACT 
GGAACTTACG ACAGTGAACT TCTTGAACAG ATCTCACCAT TATTATGCAT AATATTTTCA 
CACAAGAACA AACAGATAAG AAAACAAAGT GCTCAGTTCT GGAATGCCAC ATTTGCTAAA 
GTGACGATGT TGATTTATCC TGAGGAGTTA AAGCCAATAC TGAAACAAGC CAAACAAAAA 
TCTCTGCTTT TATTGCCTGG TTTGGAAAAT GTTGAAATTA TGGAGGAATC CAGTGGACCA 
TATTCAGATA TGACAGAAAA TTCCCAATTA AATATGAAGA TAAGTGGCAT GGAAAGAAAA 
TCAAGTGGAA AAAGAGATTC TTTTTTGGCA CAAACAAAGG ATACAAAAGA CAGTATGAAA 
CCACCAGCTA AATTGAAACT AGATTCTTCA ACTCCAAAAG CAAAGGGTGA AATGCCTTTG 
GAAGAAGAAA AGTCTACTGA CTTTGTGTTT ATACCTCCAG AAGTAAAAGA AACAAAGGAA 
AGAATACTAA CTGAACACCA GAAAGAAGTA CTCAAAACAA AGAGGTGTGA TATTCCTGCC 
ATGTATAATA ATCTGGATGT TTCACAAGAT ACTTTATTTT CTCAGTATAG TCAGGAAGAA 
TCTATGGAAA TTTCTACTTT AATTGAAAAA TCAAAAGAAG ACTCCAAGAT GATATTTAAG 
GAGGAACAAA AGGAGAATGA CATTGTCGTT CCTCAACATG TCATGGAAAA CTGCGGTATG 
GATGAACATC TTGAAAAGGC TTCCCTTTTG AATAATGAGT GTGGTTCTAT TGAGGAAACC 
AGTCCAAAAA TACTGAGCAG TAATAATGAT GCCAGAAAAA AAGCTTTAAT TGCTTCAAAT 
AAACCAACAG AATGTGCATC TACTACAGAA AATCCATCTG GTGTCAGCAG TAGCTCGGTT 
TCTATTGCCA CCATTTCTGG AGCTCCCTTA CAGCCTACAA GTCGAAGGCA ATCCTTTATT 
ACTTTGGAAA AGTTTGATGG TTCAGAAAAT AGACCTTTTA GTCCATCACC CTTGAATAAT 
ATGTCATCAA CTGTAATAGT GAAAAGTAAC CAGGAAGGCA TGGGTAAAAC AGATAATCCA 
TCAAAAGCAA AGAAGAGAGA AGTGGCTCTT TCCAAATCTG ACTCTGAAAA AATAACACAT 
GGGACTAAGA GATCAAGCCG AAGGTCGAGT AAATCTGAAC CAATAGGGAG TAAAAAGTCT 
AAACCCTTAA TGAGATCTGA ACAAGAGAAA AGTACTCAAG AATCTGTTGA TGGCACAGTA 
GCCTTAGAAA ATAACCCACC TGGCTTGCTT AATCAGACAG AATGTGTGTT AGATAATCAG 
GTTCATCTCT CTGAATCTAC AGTGGAGTTT GAGGATACAA TGCTTGAATC AACAGTGGAG 
AATACTGTAT TAGAAAACAA TACTGTAGAA GAGAGAACCT TAGAAGTTAA CTTGGAATCC 
AAAGAAAACA CACCCCCAGC CATAATAACG GATCAGACAG TAAATGAGGA TAGTCATATT 
CAGGTAACTC CAAATCAGAA AACCCTTAGG CGATCTTTAA GGCGACGTTC AGAAACAGCA 
GAACCAACCA CTGATAGCCA AGATAAAGAA AATAATCATC AAAAAAGGGA AAGACGTAAA 
GAAGAAGAAA AGACTCCGCA GAAGAGTCCG TTACATGTAA AAGATGATGT TTTACCTAAG 
CAAAAATTGA TTTCTGAACA AAGTGTACAG GGAAACTTAG TTGACAAAGG AAATAATTTA 
CATGAGAAGG CTTTTGGGGA GACCAGTGCT AATGCTGAAA TTGATGAAAA TAGAAGAAAG 
CCAGATCTTG AGAATACTAA ATCTGAAGGA GATGGTGTGC CGGACATTGC AGATAAATCC 
TCTGAGAAAC CAGTAAGGGG ACGAACACGA TACCAAACAA GAAGAGCATC CCAGGGTTTA 
CTTTCCAGCA TTGAAAACTC AGAATCTGAT AGTTCTGAGG CAAAAGAAGA AGGTTCTAAA 
AAGAAAAGAT CTGGAAAATG GAAGAACAAA AGCAGTGACA ATGTTGACAT GGAAGATCAA 
GAAGAGAAAA TGGTAAAACA GGAATGTATA AAAATTGAAA ATCAGGCACA TGATTATAAA 
GGGAATCCTG AAGTAGACAA AGATATAAAA TCTCAAATCT GTGAAAAAGG AGATGAAAGT 
ACTACTGTAA GTCTTCAAGA TTCTACCGTA TCTCCAGATT TATTACAAGT TTATGATGAT 
GTATTGAATA CTTCTGAGGG AGAAAGTAAA AGTAACAAAG ATGCAGACCA TTCCTTTTCA 
AATCCTTCTG TACCAGAATC AAATCTAAGG ACTAGAAATG CCAGTAAGAG GTTACATAAG 
CGAGATTCTA TAGAAAATAG CATGGGGGAA TCCTCAAAAA TAGGGACATC AGACATTTCT 
TTGCTTTCTG AAAAAACTCT TCAGACAGTA GAATGCCAGC ACAAGAGAAG TAGGAGGGTG 
AGGCGATCTA AAGGTTGTGA TTGTTGTGGA GAAAAATCAC AGTCTCAGGA AAAGTCATTT 
ACTGGGTTAA AGGATACAGA AAATTACGAT GTAAAAGTCA GTGAAACAAA AAAGACAGAT 
GGGGAAACAC CTGTATCTAT TTCAGAAACT TCGAAAGCTG ATCTGTCCTC GGAGGTTAAA 
CTTTTAGATG AGCATCATAG TGTGAATTTT CATTTGGATC TCAAGGAAGA GAATGATACT 
ACTAATGATT CATTAATTAT ATCTGAAACT GAGTTAAAAG AAAATACTAC TCAAAGCTTT 
CTTCCTTCTG AAATGGTAAA ATTTAAAGAA ACTTCTCGTA GGGATTCTGA GGAAGCAATA 
TCTCTAGAAA CCCAGGAGCC ATCTAATAAG AAATGTAAAA CTGTTGACCC ACATTTAGAT 
GTTTCCAAAG ATATTTCACT GAAATGTTTT TCTTTGGAGA CTAAAGAAGA AAAGTCAGAG 
GCCACTTCAA AAGAGAAATT GAGTACAGAG AACATTGTTA TTGTTGAATC AAATGCAGAA 
TCAAATCCAG AAGTAGATGC TATGGAATTG AATGTAGAAA ATGATCAGTC TGAAGCTGGC 
TTCTCTGAAC AAAAGAATGT CAAAACTGGT ATTCCAGGGA AAGAAGTAAC AGAGGAAAAC 
AGTGAAAGAA TGGATACACC TGAAAAACTG AAAGCCAATG AAGCAGTAAC TGAGGAATTT 
AATTCAGCTA CTGATTATTC GGATACTCCG ACATCTGTAA AAGTGAATGC TCAGGTAGAG 
ATCTCTGAAC AAATAGCGGT TGGGGAGCTA GGTGGAGGAA GTGATGAATC TGATCCAGGC 
TCATCTGAAG AAATGAACGC TGAAATGAAA AATTGTGAAA AAACAGCGGT TTGTGAGGTG 
ACTACTGAAT CTGACCACGT CACTGAAACC GAAGACGAAG ATGTAACTAC CACGGCTTCT 
CTGCCTATAG AAGTTGAAAC AAAGGAATTA GATGTAGAAG TCAATAACTT TGTTCCCGAC 
ACACTTGAGA TGATCACTGA AGAAGGAAAG GTTGACCCTA ACAAAACTGA AACGAATACT 
GAACCTAATA AATTGGAGGA AACACAGTTA GATGACAATC AGATGGTAGT GGAAAGTAAT 
GAAACTTTAC CGGAAGTTCA CCATACTTCA GAGAAAGTAG AGGAGATAGC ACAACCTCTG 
ACATCTGAAA CAGATATTTC TGGACTAATA TCAGAAGACA GTAATACATC TCCTCAAAAA 
TCGAAGGATA TTGATCCTTT ACTCATGTCA GCAAACGAGA GTCCTAGTGG CATGCACACA 
CGTTGTGTGT GGTCTCCTTT GGCTTCTCCA TCCACCAGCA TTTTAAAGAG AGGACTAAAA 
AGACCCCAAG AAGATGAAAT CTCGTCACCT GTCCACAAGG TTCGCCGTGT CTCCTTTGCA 
GATCCTATAT ACCAAGCAGG ATTGGCAGAT GACATTGATA GGCGATGCTC TATTGTTAGG 
TGCCATTCTT CAAATAGTTC TCTCATGGTA AAAAGTGTTA AAACTTCACC TACTGTGCAA 
TCTAAGCATA ATACCACTTC AGCCAAAGGA TTTCTGTCCC CAGGATCACG TAGCTCTAAA 
TTTAAGAGCT CAAAGAAGTG TTTAATTGCA GAAATGGCTA AGGAATCTGT GCCATGCCCA 
ACAGAAAGTG TTTACCCTGC TTTGGTGAAC TGTGTAGCAC CAGTTGAAAT CATTTTACCT 
CAGATTACAT CCAACATGTG GGCAAGAGGA CTGGGACAGC TCATTAGAGC CAAGAATATA 
AAAACAATTG GCGATTTGAG TACCCTTACA GCATCTGAAA TAAAAACTCT TCCTATCCGT 
TCTCCAAAAG TGTCTAACGT TAAAAAGGCT CTCAGAATAT ATCATGAGCA GCAGGTGAAG 
TCTCGTGGAC TAGAAGAGAT TCCAGTTTTT GATATTTCTG AAAAAACAAT AAATGGAATG 
GAAAATAAAT CTATCTCTCC TGATGAAGAA AGACTTGCCT CAGATTTAAT TGATCCTGTT 
GCTTTAGAAG CTCCGTTATC TAAAAATCTT GTGGCACAGA TTAGCGCACT TGCTCTTCAA 
CTAGATTCAG AAGATCTCTA TAATTATTCA GGAAGCCAGC TATTTGAAAT GCACGAGAAA 
CTTGGTAGCA TGGCAAACTC TATAATTAAA AATCTGCAGT CACGTTGGAG GTCACCAGCC 
CATGAAAATT CTTTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RIF1 ( XM_024980695.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024836463.1 Bos taurus replication timing regulatory factor 1 (RIF1), transcript variant X6, mRNA.

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CloneID	OBa02875
Clone ID Related Accession (Same CDS sequence)	XM_010826492.2 , XM_002685359.6 , XM_002685359.5 , XM_024980692.1 , XM_024980695.1 , XM_010802076.2 , XM_615269.9
Accession Version	XM_024980695.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7398bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	telomere-associated protein RIF1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGCCG CGGATCAGAG CCCCCTGGCA CCGCTGTTGG AGACTTTGGA AGACCCTTCC 
GCCTCCCTTG GAGAGCAGAC CGACGCTTAC CTGACCCTGA CCAGTCGTAT GACTGGAGAA 
GATGGAAAAG AAATCATTAC AGAAATTGAG AAAAAACTTC CTCAGCTATT TAAAGTTTTA 
AAGACTCACA TTGCCAGTCA AAACTCAGAG CTCAGTAGTG CTGCTCTACA GGCCTTGGGG 
TTTTGCTTAT ATAATCCCAA AATTGCCTCT GGATTATCAG AGATAAATAT TCAAGAACTG 
CTTTCAAAAC TGAATGATAT TGTTAAAAGT TCAGATAAAA ATGTACGTAC TAGGGCACTT 
TGGGTGATAT CCAAGCAGAC GTTTCCCACT GAAGTCGTTG GCAAAGTAGT TTCTAGTATC 
ATTGACTCAC TAGAAATAGT ATTTAATAGA GGAGAGATGC TTTCTGCTGT TGTGGATTAT 
GAAGCATTAA ATGTCATTAT AAGACTAATT GAACAAGCCC CAGTTCAAAT GGGAGAAGAG 
TCAATTAGGT GGGCAAAATT GGTCATACCT TTACTGGTTC ATTCAGCCCA AAAAGTACAT 
TTGCGGGGAG CCACTGCTCT AGAGATGGGA ATGCCTTTGT TGCTTCAGAA ACAGCAAGAA 
ATAGCATCTA TCACAGAGCA GCTTATGACT ACCAAATTAC TTTCAGAACT CCAGAAACTG 
TTTATGAGTA AAAATGAGAC TTATGTGTTA AAATTGTGGC CTTTATTTGT CAAACTTCTT 
GGGAAGACCT TGCATCGAAG TGGGAGTTTC ATTAATTCTC TGTTACAGCT GGAAGAACTG 
GGATTTCGTA GTGGAGCACC CATGATTAAA AAAATAGCTT TTATTGCTTG GAAGAGTTTA 
ATAGATAATT TTGCTTTAAA TCCAGAAATA CTGTGTAGTG CAAAAAGACT GAAGTTGTTG 
ATGCAGCCTT TGAGTTCTAT CCATGTGAGA ACAGAAACTC TAGCGCTGAC AAAACTAGAA 
GTCTGGTGGT ATTTGTTGAT GAGACTAGGA CCTCATCTTC CTGCTAATTT TGAACAGGTT 
TGTGTGCCTC TGATTCAGAG CACAATAAGC ATTGATTCTA ATGCTTCACC TCAAGGGAGT 
TCATCTCGTG TAGCTACTAC TCCAGGTTTA AGCCCTATGA CCCCAGTACA CAAAGGTGCT 
TCTCCATATG GAACTCCTGT AACCCCTCGG ATGAACTTGA ATTCAAATTT TGGAATGGCC 
ACAATCCCCT CTATACAACT TTTGGGGCTT GAAATGTTGC TTCATTTTTT GTTGGGTCCA 
GAAGTCGTGA GTTTTGCTAA GCAAAACAAA CTTATACTGA GCCTAGAACC ACTTGAACAT 
CCATTAATCA GCAGCTCTTC TTTTTTCTCC AAACACTCAA ATACACTTAT CACTGCTGTT 
CATGATAGCT TTGTTGCAGT TGGAAAAGAT GCTTCTGATG TAGTTATTAA TGCTATTTGG 
AAGGAGTTAA TTAGCTTGGT GAAGTCAGTT ATTGAATCAG GTAACAAAAA AGAGAGACCA 
GGTTCTGAAG TTTTGACTCT CTTATTAAAA TCTTTGGAAA GCATAGTGAA TTCAGAAGTA 
TTTCCTGTAT TGAAAACACT GGTTCTCATG GAAATTACAA TTAAAGGACT TCCTCAGAAA 
GTATTAGGTT CTCCAGCATA TCAGGTTGCT AATATGGATA TTCTTAATGG AACCCCTGCT 
TTGTTCTTAA TTCAGTTAAC TTTCAATAAT AATCTATTGG AATGTGGTGT GGAAGACGAA 
AGGTTTTTTC TCAACCTGGA AACACTTGTG GGCTGTGTTC TTTCGGGTCC AACTTCACCA 
CTCGCTTTCA GTGACTCTGT CTTAAATGTT GTCAATCAAA ATGCAAAGCA GTTGGATAAC 
AAGGAGCATC TTTGGAGAAT GTGGAGTATT ATAGTCACCC CATTAACAGA ATTGATTAAT 
CAGACCAATG AAGTGAATCA AGGGGATGCC TTAGAACATA ATTTCAGTGC TATCTATGGT 
GCACTGATTT TGCCAATAAA CCATATTTTT CCAGCACAAA AAATTCCAGT GGTCACCATG 
AAGACCTTAC TTAAAACTTG GTCAGAATTA TATAGAGCAT TTGCTCGCTG TGCAGCTTTG 
GTGGCAACTG CGGAAGAGAA TTTATGCTGT GAGGAATTTT GTTCCAAGAT AATGTCCAGT 
TTGGAAGATG AAGGCCTTTC AAATTTATTG TTCTTGGATA GGATCACTCA TATTATTACT 
GTAATGGTTG ATTGCATCGA CTTTTCACCA TATAATATTA AATATCAACC CAAAACTAAA 
TCACCACAGA GACCTACAGA TTGGTCCAAA AAGAAGAAGG AACCTTTAGG GAAGTTGGCT 
TCTTTAATTA AACTTATTGT GAGAGTGATC TATTCTTTTC ACACACTGAG CCTCAAGGAA 
GTACATTCTG ATACTCTCCT CACTATTGGC AACTCAATCA CCAGCATTCT TTCCAATTTA 
CTTGGACATA TTTCTCTGCC TTCTATGATC CAGAAAATAT TTGCAGCTTT AACAAGACCT 
CTGGCATTAT TTTATGAAAA CTCAAAGCTT GATGAAGTTC CTAAAGTGTA TAGTTGTATG 
AACAACAAGT TAGAAAAGCT ACTAGGAGAT ATAATTGCTT GTCTACATGT CAGCTACACT 
GGAACTTACG ACAGTGAACT TCTTGAACAG ATCTCACCAT TATTATGCAT AATATTTTCA 
CACAAGAACA AACAGATAAG AAAACAAAGT GCTCAGTTCT GGAATGCCAC ATTTGCTAAA 
GTGACGATGT TGATTTATCC TGAGGAGTTA AAGCCAATAC TGAAACAAGC CAAACAAAAA 
TCTCTGCTTT TATTGCCTGG TTTGGAAAAT GTTGAAATTA TGGAGGAATC CAGTGGACCA 
TATTCAGATA TGACAGAAAA TTCCCAATTA AATATGAAGA TAAGTGGCAT GGAAAGAAAA 
TCAAGTGGAA AAAGAGATTC TTTTTTGGCA CAAACAAAGG ATACAAAAGA CAGTATGAAA 
CCACCAGCTA AATTGAAACT AGATTCTTCA ACTCCAAAAG CAAAGGGTGA AATGCCTTTG 
GAAGAAGAAA AGTCTACTGA CTTTGTGTTT ATACCTCCAG AAGTAAAAGA AACAAAGGAA 
AGAATACTAA CTGAACACCA GAAAGAAGTA CTCAAAACAA AGAGGTGTGA TATTCCTGCC 
ATGTATAATA ATCTGGATGT TTCACAAGAT ACTTTATTTT CTCAGTATAG TCAGGAAGAA 
TCTATGGAAA TTTCTACTTT AATTGAAAAA TCAAAAGAAG ACTCCAAGAT GATATTTAAG 
GAGGAACAAA AGGAGAATGA CATTGTCGTT CCTCAACATG TCATGGAAAA CTGCGGTATG 
GATGAACATC TTGAAAAGGC TTCCCTTTTG AATAATGAGT GTGGTTCTAT TGAGGAAACC 
AGTCCAAAAA TACTGAGCAG TAATAATGAT GCCAGAAAAA AAGCTTTAAT TGCTTCAAAT 
AAACCAACAG AATGTGCATC TACTACAGAA AATCCATCTG GTGTCAGCAG TAGCTCGGTT 
TCTATTGCCA CCATTTCTGG AGCTCCCTTA CAGCCTACAA GTCGAAGGCA ATCCTTTATT 
ACTTTGGAAA AGTTTGATGG TTCAGAAAAT AGACCTTTTA GTCCATCACC CTTGAATAAT 
ATGTCATCAA CTGTAATAGT GAAAAGTAAC CAGGAAGGCA TGGGTAAAAC AGATAATCCA 
TCAAAAGCAA AGAAGAGAGA AGTGGCTCTT TCCAAATCTG ACTCTGAAAA AATAACACAT 
GGGACTAAGA GATCAAGCCG AAGGTCGAGT AAATCTGAAC CAATAGGGAG TAAAAAGTCT 
AAACCCTTAA TGAGATCTGA ACAAGAGAAA AGTACTCAAG AATCTGTTGA TGGCACAGTA 
GCCTTAGAAA ATAACCCACC TGGCTTGCTT AATCAGACAG AATGTGTGTT AGATAATCAG 
GTTCATCTCT CTGAATCTAC AGTGGAGTTT GAGGATACAA TGCTTGAATC AACAGTGGAG 
AATACTGTAT TAGAAAACAA TACTGTAGAA GAGAGAACCT TAGAAGTTAA CTTGGAATCC 
AAAGAAAACA CACCCCCAGC CATAATAACG GATCAGACAG TAAATGAGGA TAGTCATATT 
CAGGTAACTC CAAATCAGAA AACCCTTAGG CGATCTTTAA GGCGACGTTC AGAAACAGCA 
GAACCAACCA CTGATAGCCA AGATAAAGAA AATAATCATC AAAAAAGGGA AAGACGTAAA 
GAAGAAGAAA AGACTCCGCA GAAGAGTCCG TTACATGTAA AAGATGATGT TTTACCTAAG 
CAAAAATTGA TTTCTGAACA AAGTGTACAG GGAAACTTAG TTGACAAAGG AAATAATTTA 
CATGAGAAGG CTTTTGGGGA GACCAGTGCT AATGCTGAAA TTGATGAAAA TAGAAGAAAG 
CCAGATCTTG AGAATACTAA ATCTGAAGGA GATGGTGTGC CGGACATTGC AGATAAATCC 
TCTGAGAAAC CAGTAAGGGG ACGAACACGA TACCAAACAA GAAGAGCATC CCAGGGTTTA 
CTTTCCAGCA TTGAAAACTC AGAATCTGAT AGTTCTGAGG CAAAAGAAGA AGGTTCTAAA 
AAGAAAAGAT CTGGAAAATG GAAGAACAAA AGCAGTGACA ATGTTGACAT GGAAGATCAA 
GAAGAGAAAA TGGTAAAACA GGAATGTATA AAAATTGAAA ATCAGGCACA TGATTATAAA 
GGGAATCCTG AAGTAGACAA AGATATAAAA TCTCAAATCT GTGAAAAAGG AGATGAAAGT 
ACTACTGTAA GTCTTCAAGA TTCTACCGTA TCTCCAGATT TATTACAAGT TTATGATGAT 
GTATTGAATA CTTCTGAGGG AGAAAGTAAA AGTAACAAAG ATGCAGACCA TTCCTTTTCA 
AATCCTTCTG TACCAGAATC AAATCTAAGG ACTAGAAATG CCAGTAAGAG GTTACATAAG 
CGAGATTCTA TAGAAAATAG CATGGGGGAA TCCTCAAAAA TAGGGACATC AGACATTTCT 
TTGCTTTCTG AAAAAACTCT TCAGACAGTA GAATGCCAGC ACAAGAGAAG TAGGAGGGTG 
AGGCGATCTA AAGGTTGTGA TTGTTGTGGA GAAAAATCAC AGTCTCAGGA AAAGTCATTT 
ACTGGGTTAA AGGATACAGA AAATTACGAT GTAAAAGTCA GTGAAACAAA AAAGACAGAT 
GGGGAAACAC CTGTATCTAT TTCAGAAACT TCGAAAGCTG ATCTGTCCTC GGAGGTTAAA 
CTTTTAGATG AGCATCATAG TGTGAATTTT CATTTGGATC TCAAGGAAGA GAATGATACT 
ACTAATGATT CATTAATTAT ATCTGAAACT GAGTTAAAAG AAAATACTAC TCAAAGCTTT 
CTTCCTTCTG AAATGGTAAA ATTTAAAGAA ACTTCTCGTA GGGATTCTGA GGAAGCAATA 
TCTCTAGAAA CCCAGGAGCC ATCTAATAAG AAATGTAAAA CTGTTGACCC ACATTTAGAT 
GTTTCCAAAG ATATTTCACT GAAATGTTTT TCTTTGGAGA CTAAAGAAGA AAAGTCAGAG 
GCCACTTCAA AAGAGAAATT GAGTACAGAG AACATTGTTA TTGTTGAATC AAATGCAGAA 
TCAAATCCAG AAGTAGATGC TATGGAATTG AATGTAGAAA ATGATCAGTC TGAAGCTGGC 
TTCTCTGAAC AAAAGAATGT CAAAACTGGT ATTCCAGGGA AAGAAGTAAC AGAGGAAAAC 
AGTGAAAGAA TGGATACACC TGAAAAACTG AAAGCCAATG AAGCAGTAAC TGAGGAATTT 
AATTCAGCTA CTGATTATTC GGATACTCCG ACATCTGTAA AAGTGAATGC TCAGGTAGAG 
ATCTCTGAAC AAATAGCGGT TGGGGAGCTA GGTGGAGGAA GTGATGAATC TGATCCAGGC 
TCATCTGAAG AAATGAACGC TGAAATGAAA AATTGTGAAA AAACAGCGGT TTGTGAGGTG 
ACTACTGAAT CTGACCACGT CACTGAAACC GAAGACGAAG ATGTAACTAC CACGGCTTCT 
CTGCCTATAG AAGTTGAAAC AAAGGAATTA GATGTAGAAG TCAATAACTT TGTTCCCGAC 
ACACTTGAGA TGATCACTGA AGAAGGAAAG GTTGACCCTA ACAAAACTGA AACGAATACT 
GAACCTAATA AATTGGAGGA AACACAGTTA GATGACAATC AGATGGTAGT GGAAAGTAAT 
GAAACTTTAC CGGAAGTTCA CCATACTTCA GAGAAAGTAG AGGAGATAGC ACAACCTCTG 
ACATCTGAAA CAGATATTTC TGGACTAATA TCAGAAGACA GTAATACATC TCCTCAAAAA 
TCGAAGGATA TTGATCCTTT ACTCATGTCA GCAAACGAGA GTCCTAGTGG CATGCACACA 
CGTTGTGTGT GGTCTCCTTT GGCTTCTCCA TCCACCAGCA TTTTAAAGAG AGGACTAAAA 
AGACCCCAAG AAGATGAAAT CTCGTCACCT GTCCACAAGG TTCGCCGTGT CTCCTTTGCA 
GATCCTATAT ACCAAGCAGG ATTGGCAGAT GACATTGATA GGCGATGCTC TATTGTTAGG 
TGCCATTCTT CAAATAGTTC TCTCATGGTA AAAAGTGTTA AAACTTCACC TACTGTGCAA 
TCTAAGCATA ATACCACTTC AGCCAAAGGA TTTCTGTCCC CAGGATCACG TAGCTCTAAA 
TTTAAGAGCT CAAAGAAGTG TTTAATTGCA GAAATGGCTA AGGAATCTGT GCCATGCCCA 
ACAGAAAGTG TTTACCCTGC TTTGGTGAAC TGTGTAGCAC CAGTTGAAAT CATTTTACCT 
CAGATTACAT CCAACATGTG GGCAAGAGGA CTGGGACAGC TCATTAGAGC CAAGAATATA 
AAAACAATTG GCGATTTGAG TACCCTTACA GCATCTGAAA TAAAAACTCT TCCTATCCGT 
TCTCCAAAAG TGTCTAACGT TAAAAAGGCT CTCAGAATAT ATCATGAGCA GCAGGTGAAG 
TCTCGTGGAC TAGAAGAGAT TCCAGTTTTT GATATTTCTG AAAAAACAAT AAATGGAATG 
GAAAATAAAT CTATCTCTCC TGATGAAGAA AGACTTGCCT CAGATTTAAT TGATCCTGTT 
GCTTTAGAAG CTCCGTTATC TAAAAATCTT GTGGCACAGA TTAGCGCACT TGCTCTTCAA 
CTAGATTCAG AAGATCTCTA TAATTATTCA GGAAGCCAGC TATTTGAAAT GCACGAGAAA 
CTTGGTAGCA TGGCAAACTC TATAATTAAA AATCTGCAGT CACGTTGGAG GTCACCAGCC 
CATGAAAATT CTTTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024836463.1
CDS	159..7556
Translation	MTAADQSPLAPLLETLEDPSASLGEQTDAYLTLTSRMTGEDGKEIITEIEKKLPQLFKVLKTHIASQNSELSSAALQALGFCLYNPKIASGLSEINIQELLSKLNDIVKSSDKNVRTRALWVISKQTFPTEVVGKVVSSIIDSLEIVFNRGEMLSAVVDYEALNVIIRLIEQAPVQMGEESIRWAKLVIPLLVHSAQKVHLRGATALEMGMPLLLQKQQEIASITEQLMTTKLLSELQKLFMSKNETYVLKLWPLFVKLLGKTLHRSGSFINSLLQLEELGFRSGAPMIKKIAFIAWKSLIDNFALNPEILCSAKRLKLLMQPLSSIHVRTETLALTKLEVWWYLLMRLGPHLPANFEQVCVPLIQSTISIDSNASPQGSSSRVATTPGLSPMTPVHKGASPYGTPVTPRMNLNSNFGMATIPSIQLLGLEMLLHFLLGPEVVSFAKQNKLILSLEPLEHPLISSSSFFSKHSNTLITAVHDSFVAVGKDASDVVINAIWKELISLVKSVIESGNKKERPGSEVLTLLLKSLESIVNSEVFPVLKTLVLMEITIKGLPQKVLGSPAYQVANMDILNGTPALFLIQLTFNNNLLECGVEDERFFLNLETLVGCVLSGPTSPLAFSDSVLNVVNQNAKQLDNKEHLWRMWSIIVTPLTELINQTNEVNQGDALEHNFSAIYGALILPINHIFPAQKIPVVTMKTLLKTWSELYRAFARCAALVATAEENLCCEEFCSKIMSSLEDEGLSNLLFLDRITHIITVMVDCIDFSPYNIKYQPKTKSPQRPTDWSKKKKEPLGKLASLIKLIVRVIYSFHTLSLKEVHSDTLLTIGNSITSILSNLLGHISLPSMIQKIFAALTRPLALFYENSKLDEVPKVYSCMNNKLEKLLGDIIACLHVSYTGTYDSELLEQISPLLCIIFSHKNKQIRKQSAQFWNATFAKVTMLIYPEELKPILKQAKQKSLLLLPGLENVEIMEESSGPYSDMTENSQLNMKISGMERKSSGKRDSFLAQTKDTKDSMKPPAKLKLDSSTPKAKGEMPLEEEKSTDFVFIPPEVKETKERILTEHQKEVLKTKRCDIPAMYNNLDVSQDTLFSQYSQEESMEISTLIEKSKEDSKMIFKEEQKENDIVVPQHVMENCGMDEHLEKASLLNNECGSIEETSPKILSSNNDARKKALIASNKPTECASTTENPSGVSSSSVSIATISGAPLQPTSRRQSFITLEKFDGSENRPFSPSPLNNMSSTVIVKSNQEGMGKTDNPSKAKKREVALSKSDSEKITHGTKRSSRRSSKSEPIGSKKSKPLMRSEQEKSTQESVDGTVALENNPPGLLNQTECVLDNQVHLSESTVEFEDTMLESTVENTVLENNTVEERTLEVNLESKENTPPAIITDQTVNEDSHIQVTPNQKTLRRSLRRRSETAEPTTDSQDKENNHQKRERRKEEEKTPQKSPLHVKDDVLPKQKLISEQSVQGNLVDKGNNLHEKAFGETSANAEIDENRRKPDLENTKSEGDGVPDIADKSSEKPVRGRTRYQTRRASQGLLSSIENSESDSSEAKEEGSKKKRSGKWKNKSSDNVDMEDQEEKMVKQECIKIENQAHDYKGNPEVDKDIKSQICEKGDESTTVSLQDSTVSPDLLQVYDDVLNTSEGESKSNKDADHSFSNPSVPESNLRTRNASKRLHKRDSIENSMGESSKIGTSDISLLSEKTLQTVECQHKRSRRVRRSKGCDCCGEKSQSQEKSFTGLKDTENYDVKVSETKKTDGETPVSISETSKADLSSEVKLLDEHHSVNFHLDLKEENDTTNDSLIISETELKENTTQSFLPSEMVKFKETSRRDSEEAISLETQEPSNKKCKTVDPHLDVSKDISLKCFSLETKEEKSEATSKEKLSTENIVIVESNAESNPEVDAMELNVENDQSEAGFSEQKNVKTGIPGKEVTEENSERMDTPEKLKANEAVTEEFNSATDYSDTPTSVKVNAQVEISEQIAVGELGGGSDESDPGSSEEMNAEMKNCEKTAVCEVTTESDHVTETEDEDVTTTASLPIEVETKELDVEVNNFVPDTLEMITEEGKVDPNKTETNTEPNKLEETQLDDNQMVVESNETLPEVHHTSEKVEEIAQPLTSETDISGLISEDSNTSPQKSKDIDPLLMSANESPSGMHTRCVWSPLASPSTSILKRGLKRPQEDEISSPVHKVRRVSFADPIYQAGLADDIDRRCSIVRCHSSNSSLMVKSVKTSPTVQSKHNTTSAKGFLSPGSRSSKFKSSKKCLIAEMAKESVPCPTESVYPALVNCVAPVEIILPQITSNMWARGLGQLIRAKNIKTIGDLSTLTASEIKTLPIRSPKVSNVKKALRIYHEQQVKSRGLEEIPVFDISEKTINGMENKSISPDEERLASDLIDPVALEAPLSKNLVAQISALALQLDSEDLYNYSGSQLFEMHEKLGSMANSIIKNLQSRWRSPAHENSF

Target ORF information:

RefSeq Version	XM_024980695.1
Organism	Bos taurus(cattle)
Definition	Bos taurus replication timing regulatory factor 1 (RIF1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024980695.1

ORF Insert Sequence:

ATGACGGCCG CGGATCAGAG CCCCCTGGCA CCGCTGTTGG AGACTTTGGA AGACCCTTCC 
GCCTCCCTTG GAGAGCAGAC CGACGCTTAC CTGACCCTGA CCAGTCGTAT GACTGGAGAA 
GATGGAAAAG AAATCATTAC AGAAATTGAG AAAAAACTTC CTCAGCTATT TAAAGTTTTA 
AAGACTCACA TTGCCAGTCA AAACTCAGAG CTCAGTAGTG CTGCTCTACA GGCCTTGGGG 
TTTTGCTTAT ATAATCCCAA AATTGCCTCT GGATTATCAG AGATAAATAT TCAAGAACTG 
CTTTCAAAAC TGAATGATAT TGTTAAAAGT TCAGATAAAA ATGTACGTAC TAGGGCACTT 
TGGGTGATAT CCAAGCAGAC GTTTCCCACT GAAGTCGTTG GCAAAGTAGT TTCTAGTATC 
ATTGACTCAC TAGAAATAGT ATTTAATAGA GGAGAGATGC TTTCTGCTGT TGTGGATTAT 
GAAGCATTAA ATGTCATTAT AAGACTAATT GAACAAGCCC CAGTTCAAAT GGGAGAAGAG 
TCAATTAGGT GGGCAAAATT GGTCATACCT TTACTGGTTC ATTCAGCCCA AAAAGTACAT 
TTGCGGGGAG CCACTGCTCT AGAGATGGGA ATGCCTTTGT TGCTTCAGAA ACAGCAAGAA 
ATAGCATCTA TCACAGAGCA GCTTATGACT ACCAAATTAC TTTCAGAACT CCAGAAACTG 
TTTATGAGTA AAAATGAGAC TTATGTGTTA AAATTGTGGC CTTTATTTGT CAAACTTCTT 
GGGAAGACCT TGCATCGAAG TGGGAGTTTC ATTAATTCTC TGTTACAGCT GGAAGAACTG 
GGATTTCGTA GTGGAGCACC CATGATTAAA AAAATAGCTT TTATTGCTTG GAAGAGTTTA 
ATAGATAATT TTGCTTTAAA TCCAGAAATA CTGTGTAGTG CAAAAAGACT GAAGTTGTTG 
ATGCAGCCTT TGAGTTCTAT CCATGTGAGA ACAGAAACTC TAGCGCTGAC AAAACTAGAA 
GTCTGGTGGT ATTTGTTGAT GAGACTAGGA CCTCATCTTC CTGCTAATTT TGAACAGGTT 
TGTGTGCCTC TGATTCAGAG CACAATAAGC ATTGATTCTA ATGCTTCACC TCAAGGGAGT 
TCATCTCGTG TAGCTACTAC TCCAGGTTTA AGCCCTATGA CCCCAGTACA CAAAGGTGCT 
TCTCCATATG GAACTCCTGT AACCCCTCGG ATGAACTTGA ATTCAAATTT TGGAATGGCC 
ACAATCCCCT CTATACAACT TTTGGGGCTT GAAATGTTGC TTCATTTTTT GTTGGGTCCA 
GAAGTCGTGA GTTTTGCTAA GCAAAACAAA CTTATACTGA GCCTAGAACC ACTTGAACAT 
CCATTAATCA GCAGCTCTTC TTTTTTCTCC AAACACTCAA ATACACTTAT CACTGCTGTT 
CATGATAGCT TTGTTGCAGT TGGAAAAGAT GCTTCTGATG TAGTTATTAA TGCTATTTGG 
AAGGAGTTAA TTAGCTTGGT GAAGTCAGTT ATTGAATCAG GTAACAAAAA AGAGAGACCA 
GGTTCTGAAG TTTTGACTCT CTTATTAAAA TCTTTGGAAA GCATAGTGAA TTCAGAAGTA 
TTTCCTGTAT TGAAAACACT GGTTCTCATG GAAATTACAA TTAAAGGACT TCCTCAGAAA 
GTATTAGGTT CTCCAGCATA TCAGGTTGCT AATATGGATA TTCTTAATGG AACCCCTGCT 
TTGTTCTTAA TTCAGTTAAC TTTCAATAAT AATCTATTGG AATGTGGTGT GGAAGACGAA 
AGGTTTTTTC TCAACCTGGA AACACTTGTG GGCTGTGTTC TTTCGGGTCC AACTTCACCA 
CTCGCTTTCA GTGACTCTGT CTTAAATGTT GTCAATCAAA ATGCAAAGCA GTTGGATAAC 
AAGGAGCATC TTTGGAGAAT GTGGAGTATT ATAGTCACCC CATTAACAGA ATTGATTAAT 
CAGACCAATG AAGTGAATCA AGGGGATGCC TTAGAACATA ATTTCAGTGC TATCTATGGT 
GCACTGATTT TGCCAATAAA CCATATTTTT CCAGCACAAA AAATTCCAGT GGTCACCATG 
AAGACCTTAC TTAAAACTTG GTCAGAATTA TATAGAGCAT TTGCTCGCTG TGCAGCTTTG 
GTGGCAACTG CGGAAGAGAA TTTATGCTGT GAGGAATTTT GTTCCAAGAT AATGTCCAGT 
TTGGAAGATG AAGGCCTTTC AAATTTATTG TTCTTGGATA GGATCACTCA TATTATTACT 
GTAATGGTTG ATTGCATCGA CTTTTCACCA TATAATATTA AATATCAACC CAAAACTAAA 
TCACCACAGA GACCTACAGA TTGGTCCAAA AAGAAGAAGG AACCTTTAGG GAAGTTGGCT 
TCTTTAATTA AACTTATTGT GAGAGTGATC TATTCTTTTC ACACACTGAG CCTCAAGGAA 
GTACATTCTG ATACTCTCCT CACTATTGGC AACTCAATCA CCAGCATTCT TTCCAATTTA 
CTTGGACATA TTTCTCTGCC TTCTATGATC CAGAAAATAT TTGCAGCTTT AACAAGACCT 
CTGGCATTAT TTTATGAAAA CTCAAAGCTT GATGAAGTTC CTAAAGTGTA TAGTTGTATG 
AACAACAAGT TAGAAAAGCT ACTAGGAGAT ATAATTGCTT GTCTACATGT CAGCTACACT 
GGAACTTACG ACAGTGAACT TCTTGAACAG ATCTCACCAT TATTATGCAT AATATTTTCA 
CACAAGAACA AACAGATAAG AAAACAAAGT GCTCAGTTCT GGAATGCCAC ATTTGCTAAA 
GTGACGATGT TGATTTATCC TGAGGAGTTA AAGCCAATAC TGAAACAAGC CAAACAAAAA 
TCTCTGCTTT TATTGCCTGG TTTGGAAAAT GTTGAAATTA TGGAGGAATC CAGTGGACCA 
TATTCAGATA TGACAGAAAA TTCCCAATTA AATATGAAGA TAAGTGGCAT GGAAAGAAAA 
TCAAGTGGAA AAAGAGATTC TTTTTTGGCA CAAACAAAGG ATACAAAAGA CAGTATGAAA 
CCACCAGCTA AATTGAAACT AGATTCTTCA ACTCCAAAAG CAAAGGGTGA AATGCCTTTG 
GAAGAAGAAA AGTCTACTGA CTTTGTGTTT ATACCTCCAG AAGTAAAAGA AACAAAGGAA 
AGAATACTAA CTGAACACCA GAAAGAAGTA CTCAAAACAA AGAGGTGTGA TATTCCTGCC 
ATGTATAATA ATCTGGATGT TTCACAAGAT ACTTTATTTT CTCAGTATAG TCAGGAAGAA 
TCTATGGAAA TTTCTACTTT AATTGAAAAA TCAAAAGAAG ACTCCAAGAT GATATTTAAG 
GAGGAACAAA AGGAGAATGA CATTGTCGTT CCTCAACATG TCATGGAAAA CTGCGGTATG 
GATGAACATC TTGAAAAGGC TTCCCTTTTG AATAATGAGT GTGGTTCTAT TGAGGAAACC 
AGTCCAAAAA TACTGAGCAG TAATAATGAT GCCAGAAAAA AAGCTTTAAT TGCTTCAAAT 
AAACCAACAG AATGTGCATC TACTACAGAA AATCCATCTG GTGTCAGCAG TAGCTCGGTT 
TCTATTGCCA CCATTTCTGG AGCTCCCTTA CAGCCTACAA GTCGAAGGCA ATCCTTTATT 
ACTTTGGAAA AGTTTGATGG TTCAGAAAAT AGACCTTTTA GTCCATCACC CTTGAATAAT 
ATGTCATCAA CTGTAATAGT GAAAAGTAAC CAGGAAGGCA TGGGTAAAAC AGATAATCCA 
TCAAAAGCAA AGAAGAGAGA AGTGGCTCTT TCCAAATCTG ACTCTGAAAA AATAACACAT 
GGGACTAAGA GATCAAGCCG AAGGTCGAGT AAATCTGAAC CAATAGGGAG TAAAAAGTCT 
AAACCCTTAA TGAGATCTGA ACAAGAGAAA AGTACTCAAG AATCTGTTGA TGGCACAGTA 
GCCTTAGAAA ATAACCCACC TGGCTTGCTT AATCAGACAG AATGTGTGTT AGATAATCAG 
GTTCATCTCT CTGAATCTAC AGTGGAGTTT GAGGATACAA TGCTTGAATC AACAGTGGAG 
AATACTGTAT TAGAAAACAA TACTGTAGAA GAGAGAACCT TAGAAGTTAA CTTGGAATCC 
AAAGAAAACA CACCCCCAGC CATAATAACG GATCAGACAG TAAATGAGGA TAGTCATATT 
CAGGTAACTC CAAATCAGAA AACCCTTAGG CGATCTTTAA GGCGACGTTC AGAAACAGCA 
GAACCAACCA CTGATAGCCA AGATAAAGAA AATAATCATC AAAAAAGGGA AAGACGTAAA 
GAAGAAGAAA AGACTCCGCA GAAGAGTCCG TTACATGTAA AAGATGATGT TTTACCTAAG 
CAAAAATTGA TTTCTGAACA AAGTGTACAG GGAAACTTAG TTGACAAAGG AAATAATTTA 
CATGAGAAGG CTTTTGGGGA GACCAGTGCT AATGCTGAAA TTGATGAAAA TAGAAGAAAG 
CCAGATCTTG AGAATACTAA ATCTGAAGGA GATGGTGTGC CGGACATTGC AGATAAATCC 
TCTGAGAAAC CAGTAAGGGG ACGAACACGA TACCAAACAA GAAGAGCATC CCAGGGTTTA 
CTTTCCAGCA TTGAAAACTC AGAATCTGAT AGTTCTGAGG CAAAAGAAGA AGGTTCTAAA 
AAGAAAAGAT CTGGAAAATG GAAGAACAAA AGCAGTGACA ATGTTGACAT GGAAGATCAA 
GAAGAGAAAA TGGTAAAACA GGAATGTATA AAAATTGAAA ATCAGGCACA TGATTATAAA 
GGGAATCCTG AAGTAGACAA AGATATAAAA TCTCAAATCT GTGAAAAAGG AGATGAAAGT 
ACTACTGTAA GTCTTCAAGA TTCTACCGTA TCTCCAGATT TATTACAAGT TTATGATGAT 
GTATTGAATA CTTCTGAGGG AGAAAGTAAA AGTAACAAAG ATGCAGACCA TTCCTTTTCA 
AATCCTTCTG TACCAGAATC AAATCTAAGG ACTAGAAATG CCAGTAAGAG GTTACATAAG 
CGAGATTCTA TAGAAAATAG CATGGGGGAA TCCTCAAAAA TAGGGACATC AGACATTTCT 
TTGCTTTCTG AAAAAACTCT TCAGACAGTA GAATGCCAGC ACAAGAGAAG TAGGAGGGTG 
AGGCGATCTA AAGGTTGTGA TTGTTGTGGA GAAAAATCAC AGTCTCAGGA AAAGTCATTT 
ACTGGGTTAA AGGATACAGA AAATTACGAT GTAAAAGTCA GTGAAACAAA AAAGACAGAT 
GGGGAAACAC CTGTATCTAT TTCAGAAACT TCGAAAGCTG ATCTGTCCTC GGAGGTTAAA 
CTTTTAGATG AGCATCATAG TGTGAATTTT CATTTGGATC TCAAGGAAGA GAATGATACT 
ACTAATGATT CATTAATTAT ATCTGAAACT GAGTTAAAAG AAAATACTAC TCAAAGCTTT 
CTTCCTTCTG AAATGGTAAA ATTTAAAGAA ACTTCTCGTA GGGATTCTGA GGAAGCAATA 
TCTCTAGAAA CCCAGGAGCC ATCTAATAAG AAATGTAAAA CTGTTGACCC ACATTTAGAT 
GTTTCCAAAG ATATTTCACT GAAATGTTTT TCTTTGGAGA CTAAAGAAGA AAAGTCAGAG 
GCCACTTCAA AAGAGAAATT GAGTACAGAG AACATTGTTA TTGTTGAATC AAATGCAGAA 
TCAAATCCAG AAGTAGATGC TATGGAATTG AATGTAGAAA ATGATCAGTC TGAAGCTGGC 
TTCTCTGAAC AAAAGAATGT CAAAACTGGT ATTCCAGGGA AAGAAGTAAC AGAGGAAAAC 
AGTGAAAGAA TGGATACACC TGAAAAACTG AAAGCCAATG AAGCAGTAAC TGAGGAATTT 
AATTCAGCTA CTGATTATTC GGATACTCCG ACATCTGTAA AAGTGAATGC TCAGGTAGAG 
ATCTCTGAAC AAATAGCGGT TGGGGAGCTA GGTGGAGGAA GTGATGAATC TGATCCAGGC 
TCATCTGAAG AAATGAACGC TGAAATGAAA AATTGTGAAA AAACAGCGGT TTGTGAGGTG 
ACTACTGAAT CTGACCACGT CACTGAAACC GAAGACGAAG ATGTAACTAC CACGGCTTCT 
CTGCCTATAG AAGTTGAAAC AAAGGAATTA GATGTAGAAG TCAATAACTT TGTTCCCGAC 
ACACTTGAGA TGATCACTGA AGAAGGAAAG GTTGACCCTA ACAAAACTGA AACGAATACT 
GAACCTAATA AATTGGAGGA AACACAGTTA GATGACAATC AGATGGTAGT GGAAAGTAAT 
GAAACTTTAC CGGAAGTTCA CCATACTTCA GAGAAAGTAG AGGAGATAGC ACAACCTCTG 
ACATCTGAAA CAGATATTTC TGGACTAATA TCAGAAGACA GTAATACATC TCCTCAAAAA 
TCGAAGGATA TTGATCCTTT ACTCATGTCA GCAAACGAGA GTCCTAGTGG CATGCACACA 
CGTTGTGTGT GGTCTCCTTT GGCTTCTCCA TCCACCAGCA TTTTAAAGAG AGGACTAAAA 
AGACCCCAAG AAGATGAAAT CTCGTCACCT GTCCACAAGG TTCGCCGTGT CTCCTTTGCA 
GATCCTATAT ACCAAGCAGG ATTGGCAGAT GACATTGATA GGCGATGCTC TATTGTTAGG 
TGCCATTCTT CAAATAGTTC TCTCATGGTA AAAAGTGTTA AAACTTCACC TACTGTGCAA 
TCTAAGCATA ATACCACTTC AGCCAAAGGA TTTCTGTCCC CAGGATCACG TAGCTCTAAA 
TTTAAGAGCT CAAAGAAGTG TTTAATTGCA GAAATGGCTA AGGAATCTGT GCCATGCCCA 
ACAGAAAGTG TTTACCCTGC TTTGGTGAAC TGTGTAGCAC CAGTTGAAAT CATTTTACCT 
CAGATTACAT CCAACATGTG GGCAAGAGGA CTGGGACAGC TCATTAGAGC CAAGAATATA 
AAAACAATTG GCGATTTGAG TACCCTTACA GCATCTGAAA TAAAAACTCT TCCTATCCGT 
TCTCCAAAAG TGTCTAACGT TAAAAAGGCT CTCAGAATAT ATCATGAGCA GCAGGTGAAG 
TCTCGTGGAC TAGAAGAGAT TCCAGTTTTT GATATTTCTG AAAAAACAAT AAATGGAATG 
GAAAATAAAT CTATCTCTCC TGATGAAGAA AGACTTGCCT CAGATTTAAT TGATCCTGTT 
GCTTTAGAAG CTCCGTTATC TAAAAATCTT GTGGCACAGA TTAGCGCACT TGCTCTTCAA 
CTAGATTCAG AAGATCTCTA TAATTATTCA GGAAGCCAGC TATTTGAAAT GCACGAGAAA 
CTTGGTAGCA TGGCAAACTC TATAATTAAA AATCTGCAGT CACGTTGGAG GTCACCAGCC 
CATGAAAATT CTTTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RIF1 ( XM_010802076.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010800378.1 Bos taurus replication timing regulatory factor 1 (RIF1), transcript variant X5, mRNA.

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CloneID	OBa02875
Clone ID Related Accession (Same CDS sequence)	XM_010826492.2 , XM_002685359.6 , XM_002685359.5 , XM_024980692.1 , XM_024980695.1 , XM_010802076.2 , XM_615269.9
Accession Version	XM_010802076.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7398bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	telomere-associated protein RIF1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103838.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010802076.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGCCG CGGATCAGAG CCCCCTGGCA CCGCTGTTGG AGACTTTGGA AGACCCTTCC 
GCCTCCCTTG GAGAGCAGAC CGACGCTTAC CTGACCCTGA CCAGTCGTAT GACTGGAGAA 
GATGGAAAAG AAATCATTAC AGAAATTGAG AAAAAACTTC CTCAGCTATT TAAAGTTTTA 
AAGACTCACA TTGCCAGTCA AAACTCAGAG CTCAGTAGTG CTGCTCTACA GGCCTTGGGG 
TTTTGCTTAT ATAATCCCAA AATTGCCTCT GGATTATCAG AGATAAATAT TCAAGAACTG 
CTTTCAAAAC TGAATGATAT TGTTAAAAGT TCAGATAAAA ATGTACGTAC TAGGGCACTT 
TGGGTGATAT CCAAGCAGAC GTTTCCCACT GAAGTCGTTG GCAAAGTAGT TTCTAGTATC 
ATTGACTCAC TAGAAATAGT ATTTAATAGA GGAGAGATGC TTTCTGCTGT TGTGGATTAT 
GAAGCATTAA ATGTCATTAT AAGACTAATT GAACAAGCCC CAGTTCAAAT GGGAGAAGAG 
TCAATTAGGT GGGCAAAATT GGTCATACCT TTACTGGTTC ATTCAGCCCA AAAAGTACAT 
TTGCGGGGAG CCACTGCTCT AGAGATGGGA ATGCCTTTGT TGCTTCAGAA ACAGCAAGAA 
ATAGCATCTA TCACAGAGCA GCTTATGACT ACCAAATTAC TTTCAGAACT CCAGAAACTG 
TTTATGAGTA AAAATGAGAC TTATGTGTTA AAATTGTGGC CTTTATTTGT CAAACTTCTT 
GGGAAGACCT TGCATCGAAG TGGGAGTTTC ATTAATTCTC TGTTACAGCT GGAAGAACTG 
GGATTTCGTA GTGGAGCACC CATGATTAAA AAAATAGCTT TTATTGCTTG GAAGAGTTTA 
ATAGATAATT TTGCTTTAAA TCCAGAAATA CTGTGTAGTG CAAAAAGACT GAAGTTGTTG 
ATGCAGCCTT TGAGTTCTAT CCATGTGAGA ACAGAAACTC TAGCGCTGAC AAAACTAGAA 
GTCTGGTGGT ATTTGTTGAT GAGACTAGGA CCTCATCTTC CTGCTAATTT TGAACAGGTT 
TGTGTGCCTC TGATTCAGAG CACAATAAGC ATTGATTCTA ATGCTTCACC TCAAGGGAGT 
TCATCTCGTG TAGCTACTAC TCCAGGTTTA AGCCCTATGA CCCCAGTACA CAAAGGTGCT 
TCTCCATATG GAACTCCTGT AACCCCTCGG ATGAACTTGA ATTCAAATTT TGGAATGGCC 
ACAATCCCCT CTATACAACT TTTGGGGCTT GAAATGTTGC TTCATTTTTT GTTGGGTCCA 
GAAGTCGTGA GTTTTGCTAA GCAAAACAAA CTTATACTGA GCCTAGAACC ACTTGAACAT 
CCATTAATCA GCAGCTCTTC TTTTTTCTCC AAACACTCAA ATACACTTAT CACTGCTGTT 
CATGATAGCT TTGTTGCAGT TGGAAAAGAT GCTTCTGATG TAGTTATTAA TGCTATTTGG 
AAGGAGTTAA TTAGCTTGGT GAAGTCAGTT ATTGAATCAG GTAACAAAAA AGAGAGACCA 
GGTTCTGAAG TTTTGACTCT CTTATTAAAA TCTTTGGAAA GCATAGTGAA TTCAGAAGTA 
TTTCCTGTAT TGAAAACACT GGTTCTCATG GAAATTACAA TTAAAGGACT TCCTCAGAAA 
GTATTAGGTT CTCCAGCATA TCAGGTTGCT AATATGGATA TTCTTAATGG AACCCCTGCT 
TTGTTCTTAA TTCAGTTAAC TTTCAATAAT AATCTATTGG AATGTGGTGT GGAAGACGAA 
AGGTTTTTTC TCAACCTGGA AACACTTGTG GGCTGTGTTC TTTCGGGTCC AACTTCACCA 
CTCGCTTTCA GTGACTCTGT CTTAAATGTT GTCAATCAAA ATGCAAAGCA GTTGGATAAC 
AAGGAGCATC TTTGGAGAAT GTGGAGTATT ATAGTCACCC CATTAACAGA ATTGATTAAT 
CAGACCAATG AAGTGAATCA AGGGGATGCC TTAGAACATA ATTTCAGTGC TATCTATGGT 
GCACTGATTT TGCCAATAAA CCATATTTTT CCAGCACAAA AAATTCCAGT GGTCACCATG 
AAGACCTTAC TTAAAACTTG GTCAGAATTA TATAGAGCAT TTGCTCGCTG TGCAGCTTTG 
GTGGCAACTG CGGAAGAGAA TTTATGCTGT GAGGAATTTT GTTCCAAGAT AATGTCCAGT 
TTGGAAGATG AAGGCCTTTC AAATTTATTG TTCTTGGATA GGATCACTCA TATTATTACT 
GTAATGGTTG ATTGCATCGA CTTTTCACCA TATAATATTA AATATCAACC CAAAACTAAA 
TCACCACAGA GACCTACAGA TTGGTCCAAA AAGAAGAAGG AACCTTTAGG GAAGTTGGCT 
TCTTTAATTA AACTTATTGT GAGAGTGATC TATTCTTTTC ACACACTGAG CCTCAAGGAA 
GTACATTCTG ATACTCTCCT CACTATTGGC AACTCAATCA CCAGCATTCT TTCCAATTTA 
CTTGGACATA TTTCTCTGCC TTCTATGATC CAGAAAATAT TTGCAGCTTT AACAAGACCT 
CTGGCATTAT TTTATGAAAA CTCAAAGCTT GATGAAGTTC CTAAAGTGTA TAGTTGTATG 
AACAACAAGT TAGAAAAGCT ACTAGGAGAT ATAATTGCTT GTCTACATGT CAGCTACACT 
GGAACTTACG ACAGTGAACT TCTTGAACAG ATCTCACCAT TATTATGCAT AATATTTTCA 
CACAAGAACA AACAGATAAG AAAACAAAGT GCTCAGTTCT GGAATGCCAC ATTTGCTAAA 
GTGACGATGT TGATTTATCC TGAGGAGTTA AAGCCAATAC TGAAACAAGC CAAACAAAAA 
TCTCTGCTTT TATTGCCTGG TTTGGAAAAT GTTGAAATTA TGGAGGAATC CAGTGGACCA 
TATTCAGATA TGACAGAAAA TTCCCAATTA AATATGAAGA TAAGTGGCAT GGAAAGAAAA 
TCAAGTGGAA AAAGAGATTC TTTTTTGGCA CAAACAAAGG ATACAAAAGA CAGTATGAAA 
CCACCAGCTA AATTGAAACT AGATTCTTCA ACTCCAAAAG CAAAGGGTGA AATGCCTTTG 
GAAGAAGAAA AGTCTACTGA CTTTGTGTTT ATACCTCCAG AAGTAAAAGA AACAAAGGAA 
AGAATACTAA CTGAACACCA GAAAGAAGTA CTCAAAACAA AGAGGTGTGA TATTCCTGCC 
ATGTATAATA ATCTGGATGT TTCACAAGAT ACTTTATTTT CTCAGTATAG TCAGGAAGAA 
TCTATGGAAA TTTCTACTTT AATTGAAAAA TCAAAAGAAG ACTCCAAGAT GATATTTAAG 
GAGGAACAAA AGGAGAATGA CATTGTCGTT CCTCAACATG TCATGGAAAA CTGCGGTATG 
GATGAACATC TTGAAAAGGC TTCCCTTTTG AATAATGAGT GTGGTTCTAT TGAGGAAACC 
AGTCCAAAAA TACTGAGCAG TAATAATGAT GCCAGAAAAA AAGCTTTAAT TGCTTCAAAT 
AAACCAACAG AATGTGCATC TACTACAGAA AATCCATCTG GTGTCAGCAG TAGCTCGGTT 
TCTATTGCCA CCATTTCTGG AGCTCCCTTA CAGCCTACAA GTCGAAGGCA ATCCTTTATT 
ACTTTGGAAA AGTTTGATGG TTCAGAAAAT AGACCTTTTA GTCCATCACC CTTGAATAAT 
ATGTCATCAA CTGTAATAGT GAAAAGTAAC CAGGAAGGCA TGGGTAAAAC AGATAATCCA 
TCAAAAGCAA AGAAGAGAGA AGTGGCTCTT TCCAAATCTG ACTCTGAAAA AATAACACAT 
GGGACTAAGA GATCAAGCCG AAGGTCGAGT AAATCTGAAC CAATAGGGAG TAAAAAGTCT 
AAACCCTTAA TGAGATCTGA ACAAGAGAAA AGTACTCAAG AATCTGTTGA TGGCACAGTA 
GCCTTAGAAA ATAACCCACC TGGCTTGCTT AATCAGACAG AATGTGTGTT AGATAATCAG 
GTTCATCTCT CTGAATCTAC AGTGGAGTTT GAGGATACAA TGCTTGAATC AACAGTGGAG 
AATACTGTAT TAGAAAACAA TACTGTAGAA GAGAGAACCT TAGAAGTTAA CTTGGAATCC 
AAAGAAAACA CACCCCCAGC CATAATAACG GATCAGACAG TAAATGAGGA TAGTCATATT 
CAGGTAACTC CAAATCAGAA AACCCTTAGG CGATCTTTAA GGCGACGTTC AGAAACAGCA 
GAACCAACCA CTGATAGCCA AGATAAAGAA AATAATCATC AAAAAAGGGA AAGACGTAAA 
GAAGAAGAAA AGACTCCGCA GAAGAGTCCG TTACATGTAA AAGATGATGT TTTACCTAAG 
CAAAAATTGA TTTCTGAACA AAGTGTACAG GGAAACTTAG TTGACAAAGG AAATAATTTA 
CATGAGAAGG CTTTTGGGGA GACCAGTGCT AATGCTGAAA TTGATGAAAA TAGAAGAAAG 
CCAGATCTTG AGAATACTAA ATCTGAAGGA GATGGTGTGC CGGACATTGC AGATAAATCC 
TCTGAGAAAC CAGTAAGGGG ACGAACACGA TACCAAACAA GAAGAGCATC CCAGGGTTTA 
CTTTCCAGCA TTGAAAACTC AGAATCTGAT AGTTCTGAGG CAAAAGAAGA AGGTTCTAAA 
AAGAAAAGAT CTGGAAAATG GAAGAACAAA AGCAGTGACA ATGTTGACAT GGAAGATCAA 
GAAGAGAAAA TGGTAAAACA GGAATGTATA AAAATTGAAA ATCAGGCACA TGATTATAAA 
GGGAATCCTG AAGTAGACAA AGATATAAAA TCTCAAATCT GTGAAAAAGG AGATGAAAGT 
ACTACTGTAA GTCTTCAAGA TTCTACCGTA TCTCCAGATT TATTACAAGT TTATGATGAT 
GTATTGAATA CTTCTGAGGG AGAAAGTAAA AGTAACAAAG ATGCAGACCA TTCCTTTTCA 
AATCCTTCTG TACCAGAATC AAATCTAAGG ACTAGAAATG CCAGTAAGAG GTTACATAAG 
CGAGATTCTA TAGAAAATAG CATGGGGGAA TCCTCAAAAA TAGGGACATC AGACATTTCT 
TTGCTTTCTG AAAAAACTCT TCAGACAGTA GAATGCCAGC ACAAGAGAAG TAGGAGGGTG 
AGGCGATCTA AAGGTTGTGA TTGTTGTGGA GAAAAATCAC AGTCTCAGGA AAAGTCATTT 
ACTGGGTTAA AGGATACAGA AAATTACGAT GTAAAAGTCA GTGAAACAAA AAAGACAGAT 
GGGGAAACAC CTGTATCTAT TTCAGAAACT TCGAAAGCTG ATCTGTCCTC GGAGGTTAAA 
CTTTTAGATG AGCATCATAG TGTGAATTTT CATTTGGATC TCAAGGAAGA GAATGATACT 
ACTAATGATT CATTAATTAT ATCTGAAACT GAGTTAAAAG AAAATACTAC TCAAAGCTTT 
CTTCCTTCTG AAATGGTAAA ATTTAAAGAA ACTTCTCGTA GGGATTCTGA GGAAGCAATA 
TCTCTAGAAA CCCAGGAGCC ATCTAATAAG AAATGTAAAA CTGTTGACCC ACATTTAGAT 
GTTTCCAAAG ATATTTCACT GAAATGTTTT TCTTTGGAGA CTAAAGAAGA AAAGTCAGAG 
GCCACTTCAA AAGAGAAATT GAGTACAGAG AACATTGTTA TTGTTGAATC AAATGCAGAA 
TCAAATCCAG AAGTAGATGC TATGGAATTG AATGTAGAAA ATGATCAGTC TGAAGCTGGC 
TTCTCTGAAC AAAAGAATGT CAAAACTGGT ATTCCAGGGA AAGAAGTAAC AGAGGAAAAC 
AGTGAAAGAA TGGATACACC TGAAAAACTG AAAGCCAATG AAGCAGTAAC TGAGGAATTT 
AATTCAGCTA CTGATTATTC GGATACTCCG ACATCTGTAA AAGTGAATGC TCAGGTAGAG 
ATCTCTGAAC AAATAGCGGT TGGGGAGCTA GGTGGAGGAA GTGATGAATC TGATCCAGGC 
TCATCTGAAG AAATGAACGC TGAAATGAAA AATTGTGAAA AAACAGCGGT TTGTGAGGTG 
ACTACTGAAT CTGACCACGT CACTGAAACC GAAGACGAAG ATGTAACTAC CACGGCTTCT 
CTGCCTATAG AAGTTGAAAC AAAGGAATTA GATGTAGAAG TCAATAACTT TGTTCCCGAC 
ACACTTGAGA TGATCACTGA AGAAGGAAAG GTTGACCCTA ACAAAACTGA AACGAATACT 
GAACCTAATA AATTGGAGGA AACACAGTTA GATGACAATC AGATGGTAGT GGAAAGTAAT 
GAAACTTTAC CGGAAGTTCA CCATACTTCA GAGAAAGTAG AGGAGATAGC ACAACCTCTG 
ACATCTGAAA CAGATATTTC TGGACTAATA TCAGAAGACA GTAATACATC TCCTCAAAAA 
TCGAAGGATA TTGATCCTTT ACTCATGTCA GCAAACGAGA GTCCTAGTGG CATGCACACA 
CGTTGTGTGT GGTCTCCTTT GGCTTCTCCA TCCACCAGCA TTTTAAAGAG AGGACTAAAA 
AGACCCCAAG AAGATGAAAT CTCGTCACCT GTCCACAAGG TTCGCCGTGT CTCCTTTGCA 
GATCCTATAT ACCAAGCAGG ATTGGCAGAT GACATTGATA GGCGATGCTC TATTGTTAGG 
TGCCATTCTT CAAATAGTTC TCTCATGGTA AAAAGTGTTA AAACTTCACC TACTGTGCAA 
TCTAAGCATA ATACCACTTC AGCCAAAGGA TTTCTGTCCC CAGGATCACG TAGCTCTAAA 
TTTAAGAGCT CAAAGAAGTG TTTAATTGCA GAAATGGCTA AGGAATCTGT GCCATGCCCA 
ACAGAAAGTG TTTACCCTGC TTTGGTGAAC TGTGTAGCAC CAGTTGAAAT CATTTTACCT 
CAGATTACAT CCAACATGTG GGCAAGAGGA CTGGGACAGC TCATTAGAGC CAAGAATATA 
AAAACAATTG GCGATTTGAG TACCCTTACA GCATCTGAAA TAAAAACTCT TCCTATCCGT 
TCTCCAAAAG TGTCTAACGT TAAAAAGGCT CTCAGAATAT ATCATGAGCA GCAGGTGAAG 
TCTCGTGGAC TAGAAGAGAT TCCAGTTTTT GATATTTCTG AAAAAACAAT AAATGGAATG 
GAAAATAAAT CTATCTCTCC TGATGAAGAA AGACTTGCCT CAGATTTAAT TGATCCTGTT 
GCTTTAGAAG CTCCGTTATC TAAAAATCTT GTGGCACAGA TTAGCGCACT TGCTCTTCAA 
CTAGATTCAG AAGATCTCTA TAATTATTCA GGAAGCCAGC TATTTGAAAT GCACGAGAAA 
CTTGGTAGCA TGGCAAACTC TATAATTAAA AATCTGCAGT CACGTTGGAG GTCACCAGCC 
CATGAAAATT CTTTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010800378.1
CDS	288..7685
Translation	MTAADQSPLAPLLETLEDPSASLGEQTDAYLTLTSRMTGEDGKEIITEIEKKLPQLFKVLKTHIASQNSELSSAALQALGFCLYNPKIASGLSEINIQELLSKLNDIVKSSDKNVRTRALWVISKQTFPTEVVGKVVSSIIDSLEIVFNRGEMLSAVVDYEALNVIIRLIEQAPVQMGEESIRWAKLVIPLLVHSAQKVHLRGATALEMGMPLLLQKQQEIASITEQLMTTKLLSELQKLFMSKNETYVLKLWPLFVKLLGKTLHRSGSFINSLLQLEELGFRSGAPMIKKIAFIAWKSLIDNFALNPEILCSAKRLKLLMQPLSSIHVRTETLALTKLEVWWYLLMRLGPHLPANFEQVCVPLIQSTISIDSNASPQGSSSRVATTPGLSPMTPVHKGASPYGTPVTPRMNLNSNFGMATIPSIQLLGLEMLLHFLLGPEVVSFAKQNKLILSLEPLEHPLISSSSFFSKHSNTLITAVHDSFVAVGKDASDVVINAIWKELISLVKSVIESGNKKERPGSEVLTLLLKSLESIVNSEVFPVLKTLVLMEITIKGLPQKVLGSPAYQVANMDILNGTPALFLIQLTFNNNLLECGVEDERFFLNLETLVGCVLSGPTSPLAFSDSVLNVVNQNAKQLDNKEHLWRMWSIIVTPLTELINQTNEVNQGDALEHNFSAIYGALILPINHIFPAQKIPVVTMKTLLKTWSELYRAFARCAALVATAEENLCCEEFCSKIMSSLEDEGLSNLLFLDRITHIITVMVDCIDFSPYNIKYQPKTKSPQRPTDWSKKKKEPLGKLASLIKLIVRVIYSFHTLSLKEVHSDTLLTIGNSITSILSNLLGHISLPSMIQKIFAALTRPLALFYENSKLDEVPKVYSCMNNKLEKLLGDIIACLHVSYTGTYDSELLEQISPLLCIIFSHKNKQIRKQSAQFWNATFAKVTMLIYPEELKPILKQAKQKSLLLLPGLENVEIMEESSGPYSDMTENSQLNMKISGMERKSSGKRDSFLAQTKDTKDSMKPPAKLKLDSSTPKAKGEMPLEEEKSTDFVFIPPEVKETKERILTEHQKEVLKTKRCDIPAMYNNLDVSQDTLFSQYSQEESMEISTLIEKSKEDSKMIFKEEQKENDIVVPQHVMENCGMDEHLEKASLLNNECGSIEETSPKILSSNNDARKKALIASNKPTECASTTENPSGVSSSSVSIATISGAPLQPTSRRQSFITLEKFDGSENRPFSPSPLNNMSSTVIVKSNQEGMGKTDNPSKAKKREVALSKSDSEKITHGTKRSSRRSSKSEPIGSKKSKPLMRSEQEKSTQESVDGTVALENNPPGLLNQTECVLDNQVHLSESTVEFEDTMLESTVENTVLENNTVEERTLEVNLESKENTPPAIITDQTVNEDSHIQVTPNQKTLRRSLRRRSETAEPTTDSQDKENNHQKRERRKEEEKTPQKSPLHVKDDVLPKQKLISEQSVQGNLVDKGNNLHEKAFGETSANAEIDENRRKPDLENTKSEGDGVPDIADKSSEKPVRGRTRYQTRRASQGLLSSIENSESDSSEAKEEGSKKKRSGKWKNKSSDNVDMEDQEEKMVKQECIKIENQAHDYKGNPEVDKDIKSQICEKGDESTTVSLQDSTVSPDLLQVYDDVLNTSEGESKSNKDADHSFSNPSVPESNLRTRNASKRLHKRDSIENSMGESSKIGTSDISLLSEKTLQTVECQHKRSRRVRRSKGCDCCGEKSQSQEKSFTGLKDTENYDVKVSETKKTDGETPVSISETSKADLSSEVKLLDEHHSVNFHLDLKEENDTTNDSLIISETELKENTTQSFLPSEMVKFKETSRRDSEEAISLETQEPSNKKCKTVDPHLDVSKDISLKCFSLETKEEKSEATSKEKLSTENIVIVESNAESNPEVDAMELNVENDQSEAGFSEQKNVKTGIPGKEVTEENSERMDTPEKLKANEAVTEEFNSATDYSDTPTSVKVNAQVEISEQIAVGELGGGSDESDPGSSEEMNAEMKNCEKTAVCEVTTESDHVTETEDEDVTTTASLPIEVETKELDVEVNNFVPDTLEMITEEGKVDPNKTETNTEPNKLEETQLDDNQMVVESNETLPEVHHTSEKVEEIAQPLTSETDISGLISEDSNTSPQKSKDIDPLLMSANESPSGMHTRCVWSPLASPSTSILKRGLKRPQEDEISSPVHKVRRVSFADPIYQAGLADDIDRRCSIVRCHSSNSSLMVKSVKTSPTVQSKHNTTSAKGFLSPGSRSSKFKSSKKCLIAEMAKESVPCPTESVYPALVNCVAPVEIILPQITSNMWARGLGQLIRAKNIKTIGDLSTLTASEIKTLPIRSPKVSNVKKALRIYHEQQVKSRGLEEIPVFDISEKTINGMENKSISPDEERLASDLIDPVALEAPLSKNLVAQISALALQLDSEDLYNYSGSQLFEMHEKLGSMANSIIKNLQSRWRSPAHENSF

Target ORF information:

RefSeq Version	XM_010802076.2
Organism	Bos taurus(cattle)
Definition	Bos taurus replication timing regulatory factor 1 (RIF1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010802076.2

ORF Insert Sequence:

ATGACGGCCG CGGATCAGAG CCCCCTGGCA CCGCTGTTGG AGACTTTGGA AGACCCTTCC 
GCCTCCCTTG GAGAGCAGAC CGACGCTTAC CTGACCCTGA CCAGTCGTAT GACTGGAGAA 
GATGGAAAAG AAATCATTAC AGAAATTGAG AAAAAACTTC CTCAGCTATT TAAAGTTTTA 
AAGACTCACA TTGCCAGTCA AAACTCAGAG CTCAGTAGTG CTGCTCTACA GGCCTTGGGG 
TTTTGCTTAT ATAATCCCAA AATTGCCTCT GGATTATCAG AGATAAATAT TCAAGAACTG 
CTTTCAAAAC TGAATGATAT TGTTAAAAGT TCAGATAAAA ATGTACGTAC TAGGGCACTT 
TGGGTGATAT CCAAGCAGAC GTTTCCCACT GAAGTCGTTG GCAAAGTAGT TTCTAGTATC 
ATTGACTCAC TAGAAATAGT ATTTAATAGA GGAGAGATGC TTTCTGCTGT TGTGGATTAT 
GAAGCATTAA ATGTCATTAT AAGACTAATT GAACAAGCCC CAGTTCAAAT GGGAGAAGAG 
TCAATTAGGT GGGCAAAATT GGTCATACCT TTACTGGTTC ATTCAGCCCA AAAAGTACAT 
TTGCGGGGAG CCACTGCTCT AGAGATGGGA ATGCCTTTGT TGCTTCAGAA ACAGCAAGAA 
ATAGCATCTA TCACAGAGCA GCTTATGACT ACCAAATTAC TTTCAGAACT CCAGAAACTG 
TTTATGAGTA AAAATGAGAC TTATGTGTTA AAATTGTGGC CTTTATTTGT CAAACTTCTT 
GGGAAGACCT TGCATCGAAG TGGGAGTTTC ATTAATTCTC TGTTACAGCT GGAAGAACTG 
GGATTTCGTA GTGGAGCACC CATGATTAAA AAAATAGCTT TTATTGCTTG GAAGAGTTTA 
ATAGATAATT TTGCTTTAAA TCCAGAAATA CTGTGTAGTG CAAAAAGACT GAAGTTGTTG 
ATGCAGCCTT TGAGTTCTAT CCATGTGAGA ACAGAAACTC TAGCGCTGAC AAAACTAGAA 
GTCTGGTGGT ATTTGTTGAT GAGACTAGGA CCTCATCTTC CTGCTAATTT TGAACAGGTT 
TGTGTGCCTC TGATTCAGAG CACAATAAGC ATTGATTCTA ATGCTTCACC TCAAGGGAGT 
TCATCTCGTG TAGCTACTAC TCCAGGTTTA AGCCCTATGA CCCCAGTACA CAAAGGTGCT 
TCTCCATATG GAACTCCTGT AACCCCTCGG ATGAACTTGA ATTCAAATTT TGGAATGGCC 
ACAATCCCCT CTATACAACT TTTGGGGCTT GAAATGTTGC TTCATTTTTT GTTGGGTCCA 
GAAGTCGTGA GTTTTGCTAA GCAAAACAAA CTTATACTGA GCCTAGAACC ACTTGAACAT 
CCATTAATCA GCAGCTCTTC TTTTTTCTCC AAACACTCAA ATACACTTAT CACTGCTGTT 
CATGATAGCT TTGTTGCAGT TGGAAAAGAT GCTTCTGATG TAGTTATTAA TGCTATTTGG 
AAGGAGTTAA TTAGCTTGGT GAAGTCAGTT ATTGAATCAG GTAACAAAAA AGAGAGACCA 
GGTTCTGAAG TTTTGACTCT CTTATTAAAA TCTTTGGAAA GCATAGTGAA TTCAGAAGTA 
TTTCCTGTAT TGAAAACACT GGTTCTCATG GAAATTACAA TTAAAGGACT TCCTCAGAAA 
GTATTAGGTT CTCCAGCATA TCAGGTTGCT AATATGGATA TTCTTAATGG AACCCCTGCT 
TTGTTCTTAA TTCAGTTAAC TTTCAATAAT AATCTATTGG AATGTGGTGT GGAAGACGAA 
AGGTTTTTTC TCAACCTGGA AACACTTGTG GGCTGTGTTC TTTCGGGTCC AACTTCACCA 
CTCGCTTTCA GTGACTCTGT CTTAAATGTT GTCAATCAAA ATGCAAAGCA GTTGGATAAC 
AAGGAGCATC TTTGGAGAAT GTGGAGTATT ATAGTCACCC CATTAACAGA ATTGATTAAT 
CAGACCAATG AAGTGAATCA AGGGGATGCC TTAGAACATA ATTTCAGTGC TATCTATGGT 
GCACTGATTT TGCCAATAAA CCATATTTTT CCAGCACAAA AAATTCCAGT GGTCACCATG 
AAGACCTTAC TTAAAACTTG GTCAGAATTA TATAGAGCAT TTGCTCGCTG TGCAGCTTTG 
GTGGCAACTG CGGAAGAGAA TTTATGCTGT GAGGAATTTT GTTCCAAGAT AATGTCCAGT 
TTGGAAGATG AAGGCCTTTC AAATTTATTG TTCTTGGATA GGATCACTCA TATTATTACT 
GTAATGGTTG ATTGCATCGA CTTTTCACCA TATAATATTA AATATCAACC CAAAACTAAA 
TCACCACAGA GACCTACAGA TTGGTCCAAA AAGAAGAAGG AACCTTTAGG GAAGTTGGCT 
TCTTTAATTA AACTTATTGT GAGAGTGATC TATTCTTTTC ACACACTGAG CCTCAAGGAA 
GTACATTCTG ATACTCTCCT CACTATTGGC AACTCAATCA CCAGCATTCT TTCCAATTTA 
CTTGGACATA TTTCTCTGCC TTCTATGATC CAGAAAATAT TTGCAGCTTT AACAAGACCT 
CTGGCATTAT TTTATGAAAA CTCAAAGCTT GATGAAGTTC CTAAAGTGTA TAGTTGTATG 
AACAACAAGT TAGAAAAGCT ACTAGGAGAT ATAATTGCTT GTCTACATGT CAGCTACACT 
GGAACTTACG ACAGTGAACT TCTTGAACAG ATCTCACCAT TATTATGCAT AATATTTTCA 
CACAAGAACA AACAGATAAG AAAACAAAGT GCTCAGTTCT GGAATGCCAC ATTTGCTAAA 
GTGACGATGT TGATTTATCC TGAGGAGTTA AAGCCAATAC TGAAACAAGC CAAACAAAAA 
TCTCTGCTTT TATTGCCTGG TTTGGAAAAT GTTGAAATTA TGGAGGAATC CAGTGGACCA 
TATTCAGATA TGACAGAAAA TTCCCAATTA AATATGAAGA TAAGTGGCAT GGAAAGAAAA 
TCAAGTGGAA AAAGAGATTC TTTTTTGGCA CAAACAAAGG ATACAAAAGA CAGTATGAAA 
CCACCAGCTA AATTGAAACT AGATTCTTCA ACTCCAAAAG CAAAGGGTGA AATGCCTTTG 
GAAGAAGAAA AGTCTACTGA CTTTGTGTTT ATACCTCCAG AAGTAAAAGA AACAAAGGAA 
AGAATACTAA CTGAACACCA GAAAGAAGTA CTCAAAACAA AGAGGTGTGA TATTCCTGCC 
ATGTATAATA ATCTGGATGT TTCACAAGAT ACTTTATTTT CTCAGTATAG TCAGGAAGAA 
TCTATGGAAA TTTCTACTTT AATTGAAAAA TCAAAAGAAG ACTCCAAGAT GATATTTAAG 
GAGGAACAAA AGGAGAATGA CATTGTCGTT CCTCAACATG TCATGGAAAA CTGCGGTATG 
GATGAACATC TTGAAAAGGC TTCCCTTTTG AATAATGAGT GTGGTTCTAT TGAGGAAACC 
AGTCCAAAAA TACTGAGCAG TAATAATGAT GCCAGAAAAA AAGCTTTAAT TGCTTCAAAT 
AAACCAACAG AATGTGCATC TACTACAGAA AATCCATCTG GTGTCAGCAG TAGCTCGGTT 
TCTATTGCCA CCATTTCTGG AGCTCCCTTA CAGCCTACAA GTCGAAGGCA ATCCTTTATT 
ACTTTGGAAA AGTTTGATGG TTCAGAAAAT AGACCTTTTA GTCCATCACC CTTGAATAAT 
ATGTCATCAA CTGTAATAGT GAAAAGTAAC CAGGAAGGCA TGGGTAAAAC AGATAATCCA 
TCAAAAGCAA AGAAGAGAGA AGTGGCTCTT TCCAAATCTG ACTCTGAAAA AATAACACAT 
GGGACTAAGA GATCAAGCCG AAGGTCGAGT AAATCTGAAC CAATAGGGAG TAAAAAGTCT 
AAACCCTTAA TGAGATCTGA ACAAGAGAAA AGTACTCAAG AATCTGTTGA TGGCACAGTA 
GCCTTAGAAA ATAACCCACC TGGCTTGCTT AATCAGACAG AATGTGTGTT AGATAATCAG 
GTTCATCTCT CTGAATCTAC AGTGGAGTTT GAGGATACAA TGCTTGAATC AACAGTGGAG 
AATACTGTAT TAGAAAACAA TACTGTAGAA GAGAGAACCT TAGAAGTTAA CTTGGAATCC 
AAAGAAAACA CACCCCCAGC CATAATAACG GATCAGACAG TAAATGAGGA TAGTCATATT 
CAGGTAACTC CAAATCAGAA AACCCTTAGG CGATCTTTAA GGCGACGTTC AGAAACAGCA 
GAACCAACCA CTGATAGCCA AGATAAAGAA AATAATCATC AAAAAAGGGA AAGACGTAAA 
GAAGAAGAAA AGACTCCGCA GAAGAGTCCG TTACATGTAA AAGATGATGT TTTACCTAAG 
CAAAAATTGA TTTCTGAACA AAGTGTACAG GGAAACTTAG TTGACAAAGG AAATAATTTA 
CATGAGAAGG CTTTTGGGGA GACCAGTGCT AATGCTGAAA TTGATGAAAA TAGAAGAAAG 
CCAGATCTTG AGAATACTAA ATCTGAAGGA GATGGTGTGC CGGACATTGC AGATAAATCC 
TCTGAGAAAC CAGTAAGGGG ACGAACACGA TACCAAACAA GAAGAGCATC CCAGGGTTTA 
CTTTCCAGCA TTGAAAACTC AGAATCTGAT AGTTCTGAGG CAAAAGAAGA AGGTTCTAAA 
AAGAAAAGAT CTGGAAAATG GAAGAACAAA AGCAGTGACA ATGTTGACAT GGAAGATCAA 
GAAGAGAAAA TGGTAAAACA GGAATGTATA AAAATTGAAA ATCAGGCACA TGATTATAAA 
GGGAATCCTG AAGTAGACAA AGATATAAAA TCTCAAATCT GTGAAAAAGG AGATGAAAGT 
ACTACTGTAA GTCTTCAAGA TTCTACCGTA TCTCCAGATT TATTACAAGT TTATGATGAT 
GTATTGAATA CTTCTGAGGG AGAAAGTAAA AGTAACAAAG ATGCAGACCA TTCCTTTTCA 
AATCCTTCTG TACCAGAATC AAATCTAAGG ACTAGAAATG CCAGTAAGAG GTTACATAAG 
CGAGATTCTA TAGAAAATAG CATGGGGGAA TCCTCAAAAA TAGGGACATC AGACATTTCT 
TTGCTTTCTG AAAAAACTCT TCAGACAGTA GAATGCCAGC ACAAGAGAAG TAGGAGGGTG 
AGGCGATCTA AAGGTTGTGA TTGTTGTGGA GAAAAATCAC AGTCTCAGGA AAAGTCATTT 
ACTGGGTTAA AGGATACAGA AAATTACGAT GTAAAAGTCA GTGAAACAAA AAAGACAGAT 
GGGGAAACAC CTGTATCTAT TTCAGAAACT TCGAAAGCTG ATCTGTCCTC GGAGGTTAAA 
CTTTTAGATG AGCATCATAG TGTGAATTTT CATTTGGATC TCAAGGAAGA GAATGATACT 
ACTAATGATT CATTAATTAT ATCTGAAACT GAGTTAAAAG AAAATACTAC TCAAAGCTTT 
CTTCCTTCTG AAATGGTAAA ATTTAAAGAA ACTTCTCGTA GGGATTCTGA GGAAGCAATA 
TCTCTAGAAA CCCAGGAGCC ATCTAATAAG AAATGTAAAA CTGTTGACCC ACATTTAGAT 
GTTTCCAAAG ATATTTCACT GAAATGTTTT TCTTTGGAGA CTAAAGAAGA AAAGTCAGAG 
GCCACTTCAA AAGAGAAATT GAGTACAGAG AACATTGTTA TTGTTGAATC AAATGCAGAA 
TCAAATCCAG AAGTAGATGC TATGGAATTG AATGTAGAAA ATGATCAGTC TGAAGCTGGC 
TTCTCTGAAC AAAAGAATGT CAAAACTGGT ATTCCAGGGA AAGAAGTAAC AGAGGAAAAC 
AGTGAAAGAA TGGATACACC TGAAAAACTG AAAGCCAATG AAGCAGTAAC TGAGGAATTT 
AATTCAGCTA CTGATTATTC GGATACTCCG ACATCTGTAA AAGTGAATGC TCAGGTAGAG 
ATCTCTGAAC AAATAGCGGT TGGGGAGCTA GGTGGAGGAA GTGATGAATC TGATCCAGGC 
TCATCTGAAG AAATGAACGC TGAAATGAAA AATTGTGAAA AAACAGCGGT TTGTGAGGTG 
ACTACTGAAT CTGACCACGT CACTGAAACC GAAGACGAAG ATGTAACTAC CACGGCTTCT 
CTGCCTATAG AAGTTGAAAC AAAGGAATTA GATGTAGAAG TCAATAACTT TGTTCCCGAC 
ACACTTGAGA TGATCACTGA AGAAGGAAAG GTTGACCCTA ACAAAACTGA AACGAATACT 
GAACCTAATA AATTGGAGGA AACACAGTTA GATGACAATC AGATGGTAGT GGAAAGTAAT 
GAAACTTTAC CGGAAGTTCA CCATACTTCA GAGAAAGTAG AGGAGATAGC ACAACCTCTG 
ACATCTGAAA CAGATATTTC TGGACTAATA TCAGAAGACA GTAATACATC TCCTCAAAAA 
TCGAAGGATA TTGATCCTTT ACTCATGTCA GCAAACGAGA GTCCTAGTGG CATGCACACA 
CGTTGTGTGT GGTCTCCTTT GGCTTCTCCA TCCACCAGCA TTTTAAAGAG AGGACTAAAA 
AGACCCCAAG AAGATGAAAT CTCGTCACCT GTCCACAAGG TTCGCCGTGT CTCCTTTGCA 
GATCCTATAT ACCAAGCAGG ATTGGCAGAT GACATTGATA GGCGATGCTC TATTGTTAGG 
TGCCATTCTT CAAATAGTTC TCTCATGGTA AAAAGTGTTA AAACTTCACC TACTGTGCAA 
TCTAAGCATA ATACCACTTC AGCCAAAGGA TTTCTGTCCC CAGGATCACG TAGCTCTAAA 
TTTAAGAGCT CAAAGAAGTG TTTAATTGCA GAAATGGCTA AGGAATCTGT GCCATGCCCA 
ACAGAAAGTG TTTACCCTGC TTTGGTGAAC TGTGTAGCAC CAGTTGAAAT CATTTTACCT 
CAGATTACAT CCAACATGTG GGCAAGAGGA CTGGGACAGC TCATTAGAGC CAAGAATATA 
AAAACAATTG GCGATTTGAG TACCCTTACA GCATCTGAAA TAAAAACTCT TCCTATCCGT 
TCTCCAAAAG TGTCTAACGT TAAAAAGGCT CTCAGAATAT ATCATGAGCA GCAGGTGAAG 
TCTCGTGGAC TAGAAGAGAT TCCAGTTTTT GATATTTCTG AAAAAACAAT AAATGGAATG 
GAAAATAAAT CTATCTCTCC TGATGAAGAA AGACTTGCCT CAGATTTAAT TGATCCTGTT 
GCTTTAGAAG CTCCGTTATC TAAAAATCTT GTGGCACAGA TTAGCGCACT TGCTCTTCAA 
CTAGATTCAG AAGATCTCTA TAATTATTCA GGAAGCCAGC TATTTGAAAT GCACGAGAAA 
CTTGGTAGCA TGGCAAACTC TATAATTAAA AATCTGCAGT CACGTTGGAG GTCACCAGCC 
CATGAAAATT CTTTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RIF1 ( XM_615269.9 ) cDNA ORF clone, Bos taurus(cattle) -> XP_615269.3 Bos taurus replication timing regulatory factor 1 (RIF1), transcript variant X1, mRNA.

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CloneID	OBa02875
Clone ID Related Accession (Same CDS sequence)	XM_010826492.2 , XM_002685359.6 , XM_002685359.5 , XM_024980692.1 , XM_024980695.1 , XM_010802076.2 , XM_615269.9
Accession Version	XM_615269.9 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7398bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	telomere-associated protein RIF1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644591.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_615269.8. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGCCG CGGATCAGAG CCCCCTGGCA CCGCTGTTGG AGACTTTGGA AGACCCTTCC 
GCCTCCCTTG GAGAGCAGAC CGACGCTTAC CTGACCCTGA CCAGTCGTAT GACTGGAGAA 
GATGGAAAAG AAATCATTAC AGAAATTGAG AAAAAACTTC CTCAGCTATT TAAAGTTTTA 
AAGACTCACA TTGCCAGTCA AAACTCAGAG CTCAGTAGTG CTGCTCTACA GGCCTTGGGG 
TTTTGCTTAT ATAATCCCAA AATTGCCTCT GGATTATCAG AGATAAATAT TCAAGAACTG 
CTTTCAAAAC TGAATGATAT TGTTAAAAGT TCAGATAAAA ATGTACGTAC TAGGGCACTT 
TGGGTGATAT CCAAGCAGAC GTTTCCCACT GAAGTCGTTG GCAAAGTAGT TTCTAGTATC 
ATTGACTCAC TAGAAATAGT ATTTAATAGA GGAGAGATGC TTTCTGCTGT TGTGGATTAT 
GAAGCATTAA ATGTCATTAT AAGACTAATT GAACAAGCCC CAGTTCAAAT GGGAGAAGAG 
TCAATTAGGT GGGCAAAATT GGTCATACCT TTACTGGTTC ATTCAGCCCA AAAAGTACAT 
TTGCGGGGAG CCACTGCTCT AGAGATGGGA ATGCCTTTGT TGCTTCAGAA ACAGCAAGAA 
ATAGCATCTA TCACAGAGCA GCTTATGACT ACCAAATTAC TTTCAGAACT CCAGAAACTG 
TTTATGAGTA AAAATGAGAC TTATGTGTTA AAATTGTGGC CTTTATTTGT CAAACTTCTT 
GGGAAGACCT TGCATCGAAG TGGGAGTTTC ATTAATTCTC TGTTACAGCT GGAAGAACTG 
GGATTTCGTA GTGGAGCACC CATGATTAAA AAAATAGCTT TTATTGCTTG GAAGAGTTTA 
ATAGATAATT TTGCTTTAAA TCCAGAAATA CTGTGTAGTG CAAAAAGACT GAAGTTGTTG 
ATGCAGCCTT TGAGTTCTAT CCATGTGAGA ACAGAAACTC TAGCGCTGAC AAAACTAGAA 
GTCTGGTGGT ATTTGTTGAT GAGACTAGGA CCTCATCTTC CTGCTAATTT TGAACAGGTT 
TGTGTGCCTC TGATTCAGAG CACAATAAGC ATTGATTCTA ATGCTTCACC TCAAGGGAGT 
TCATCTCGTG TAGCTACTAC TCCAGGTTTA AGCCCTATGA CCCCAGTACA CAAAGGTGCT 
TCTCCATATG GAACTCCTGT AACCCCTCGG ATGAACTTGA ATTCAAATTT TGGAATGGCC 
ACAATCCCCT CTATACAACT TTTGGGGCTT GAAATGTTGC TTCATTTTTT GTTGGGTCCA 
GAAGTCGTGA GTTTTGCTAA GCAAAACAAA CTTATACTGA GCCTAGAACC ACTTGAACAT 
CCATTAATCA GCAGCTCTTC TTTTTTCTCC AAACACTCAA ATACACTTAT CACTGCTGTT 
CATGATAGCT TTGTTGCAGT TGGAAAAGAT GCTTCTGATG TAGTTATTAA TGCTATTTGG 
AAGGAGTTAA TTAGCTTGGT GAAGTCAGTT ATTGAATCAG GTAACAAAAA AGAGAGACCA 
GGTTCTGAAG TTTTGACTCT CTTATTAAAA TCTTTGGAAA GCATAGTGAA TTCAGAAGTA 
TTTCCTGTAT TGAAAACACT GGTTCTCATG GAAATTACAA TTAAAGGACT TCCTCAGAAA 
GTATTAGGTT CTCCAGCATA TCAGGTTGCT AATATGGATA TTCTTAATGG AACCCCTGCT 
TTGTTCTTAA TTCAGTTAAC TTTCAATAAT AATCTATTGG AATGTGGTGT GGAAGACGAA 
AGGTTTTTTC TCAACCTGGA AACACTTGTG GGCTGTGTTC TTTCGGGTCC AACTTCACCA 
CTCGCTTTCA GTGACTCTGT CTTAAATGTT GTCAATCAAA ATGCAAAGCA GTTGGATAAC 
AAGGAGCATC TTTGGAGAAT GTGGAGTATT ATAGTCACCC CATTAACAGA ATTGATTAAT 
CAGACCAATG AAGTGAATCA AGGGGATGCC TTAGAACATA ATTTCAGTGC TATCTATGGT 
GCACTGATTT TGCCAATAAA CCATATTTTT CCAGCACAAA AAATTCCAGT GGTCACCATG 
AAGACCTTAC TTAAAACTTG GTCAGAATTA TATAGAGCAT TTGCTCGCTG TGCAGCTTTG 
GTGGCAACTG CGGAAGAGAA TTTATGCTGT GAGGAATTTT GTTCCAAGAT AATGTCCAGT 
TTGGAAGATG AAGGCCTTTC AAATTTATTG TTCTTGGATA GGATCACTCA TATTATTACT 
GTAATGGTTG ATTGCATCGA CTTTTCACCA TATAATATTA AATATCAACC CAAAACTAAA 
TCACCACAGA GACCTACAGA TTGGTCCAAA AAGAAGAAGG AACCTTTAGG GAAGTTGGCT 
TCTTTAATTA AACTTATTGT GAGAGTGATC TATTCTTTTC ACACACTGAG CCTCAAGGAA 
GTACATTCTG ATACTCTCCT CACTATTGGC AACTCAATCA CCAGCATTCT TTCCAATTTA 
CTTGGACATA TTTCTCTGCC TTCTATGATC CAGAAAATAT TTGCAGCTTT AACAAGACCT 
CTGGCATTAT TTTATGAAAA CTCAAAGCTT GATGAAGTTC CTAAAGTGTA TAGTTGTATG 
AACAACAAGT TAGAAAAGCT ACTAGGAGAT ATAATTGCTT GTCTACATGT CAGCTACACT 
GGAACTTACG ACAGTGAACT TCTTGAACAG ATCTCACCAT TATTATGCAT AATATTTTCA 
CACAAGAACA AACAGATAAG AAAACAAAGT GCTCAGTTCT GGAATGCCAC ATTTGCTAAA 
GTGACGATGT TGATTTATCC TGAGGAGTTA AAGCCAATAC TGAAACAAGC CAAACAAAAA 
TCTCTGCTTT TATTGCCTGG TTTGGAAAAT GTTGAAATTA TGGAGGAATC CAGTGGACCA 
TATTCAGATA TGACAGAAAA TTCCCAATTA AATATGAAGA TAAGTGGCAT GGAAAGAAAA 
TCAAGTGGAA AAAGAGATTC TTTTTTGGCA CAAACAAAGG ATACAAAAGA CAGTATGAAA 
CCACCAGCTA AATTGAAACT AGATTCTTCA ACTCCAAAAG CAAAGGGTGA AATGCCTTTG 
GAAGAAGAAA AGTCTACTGA CTTTGTGTTT ATACCTCCAG AAGTAAAAGA AACAAAGGAA 
AGAATACTAA CTGAACACCA GAAAGAAGTA CTCAAAACAA AGAGGTGTGA TATTCCTGCC 
ATGTATAATA ATCTGGATGT TTCACAAGAT ACTTTATTTT CTCAGTATAG TCAGGAAGAA 
TCTATGGAAA TTTCTACTTT AATTGAAAAA TCAAAAGAAG ACTCCAAGAT GATATTTAAG 
GAGGAACAAA AGGAGAATGA CATTGTCGTT CCTCAACATG TCATGGAAAA CTGCGGTATG 
GATGAACATC TTGAAAAGGC TTCCCTTTTG AATAATGAGT GTGGTTCTAT TGAGGAAACC 
AGTCCAAAAA TACTGAGCAG TAATAATGAT GCCAGAAAAA AAGCTTTAAT TGCTTCAAAT 
AAACCAACAG AATGTGCATC TACTACAGAA AATCCATCTG GTGTCAGCAG TAGCTCGGTT 
TCTATTGCCA CCATTTCTGG AGCTCCCTTA CAGCCTACAA GTCGAAGGCA ATCCTTTATT 
ACTTTGGAAA AGTTTGATGG TTCAGAAAAT AGACCTTTTA GTCCATCACC CTTGAATAAT 
ATGTCATCAA CTGTAATAGT GAAAAGTAAC CAGGAAGGCA TGGGTAAAAC AGATAATCCA 
TCAAAAGCAA AGAAGAGAGA AGTGGCTCTT TCCAAATCTG ACTCTGAAAA AATAACACAT 
GGGACTAAGA GATCAAGCCG AAGGTCGAGT AAATCTGAAC CAATAGGGAG TAAAAAGTCT 
AAACCCTTAA TGAGATCTGA ACAAGAGAAA AGTACTCAAG AATCTGTTGA TGGCACAGTA 
GCCTTAGAAA ATAACCCACC TGGCTTGCTT AATCAGACAG AATGTGTGTT AGATAATCAG 
GTTCATCTCT CTGAATCTAC AGTGGAGTTT GAGGATACAA TGCTTGAATC AACAGTGGAG 
AATACTGTAT TAGAAAACAA TACTGTAGAA GAGAGAACCT TAGAAGTTAA CTTGGAATCC 
AAAGAAAACA CACCCCCAGC CATAATAACG GATCAGACAG TAAATGAGGA TAGTCATATT 
CAGGTAACTC CAAATCAGAA AACCCTTAGG CGATCTTTAA GGCGACGTTC AGAAACAGCA 
GAACCAACCA CTGATAGCCA AGATAAAGAA AATAATCATC AAAAAAGGGA AAGACGTAAA 
GAAGAAGAAA AGACTCCGCA GAAGAGTCCG TTACATGTAA AAGATGATGT TTTACCTAAG 
CAAAAATTGA TTTCTGAACA AAGTGTACAG GGAAACTTAG TTGACAAAGG AAATAATTTA 
CATGAGAAGG CTTTTGGGGA GACCAGTGCT AATGCTGAAA TTGATGAAAA TAGAAGAAAG 
CCAGATCTTG AGAATACTAA ATCTGAAGGA GATGGTGTGC CGGACATTGC AGATAAATCC 
TCTGAGAAAC CAGTAAGGGG ACGAACACGA TACCAAACAA GAAGAGCATC CCAGGGTTTA 
CTTTCCAGCA TTGAAAACTC AGAATCTGAT AGTTCTGAGG CAAAAGAAGA AGGTTCTAAA 
AAGAAAAGAT CTGGAAAATG GAAGAACAAA AGCAGTGACA ATGTTGACAT GGAAGATCAA 
GAAGAGAAAA TGGTAAAACA GGAATGTATA AAAATTGAAA ATCAGGCACA TGATTATAAA 
GGGAATCCTG AAGTAGACAA AGATATAAAA TCTCAAATCT GTGAAAAAGG AGATGAAAGT 
ACTACTGTAA GTCTTCAAGA TTCTACCGTA TCTCCAGATT TATTACAAGT TTATGATGAT 
GTATTGAATA CTTCTGAGGG AGAAAGTAAA AGTAACAAAG ATGCAGACCA TTCCTTTTCA 
AATCCTTCTG TACCAGAATC AAATCTAAGG ACTAGAAATG CCAGTAAGAG GTTACATAAG 
CGAGATTCTA TAGAAAATAG CATGGGGGAA TCCTCAAAAA TAGGGACATC AGACATTTCT 
TTGCTTTCTG AAAAAACTCT TCAGACAGTA GAATGCCAGC ACAAGAGAAG TAGGAGGGTG 
AGGCGATCTA AAGGTTGTGA TTGTTGTGGA GAAAAATCAC AGTCTCAGGA AAAGTCATTT 
ACTGGGTTAA AGGATACAGA AAATTACGAT GTAAAAGTCA GTGAAACAAA AAAGACAGAT 
GGGGAAACAC CTGTATCTAT TTCAGAAACT TCGAAAGCTG ATCTGTCCTC GGAGGTTAAA 
CTTTTAGATG AGCATCATAG TGTGAATTTT CATTTGGATC TCAAGGAAGA GAATGATACT 
ACTAATGATT CATTAATTAT ATCTGAAACT GAGTTAAAAG AAAATACTAC TCAAAGCTTT 
CTTCCTTCTG AAATGGTAAA ATTTAAAGAA ACTTCTCGTA GGGATTCTGA GGAAGCAATA 
TCTCTAGAAA CCCAGGAGCC ATCTAATAAG AAATGTAAAA CTGTTGACCC ACATTTAGAT 
GTTTCCAAAG ATATTTCACT GAAATGTTTT TCTTTGGAGA CTAAAGAAGA AAAGTCAGAG 
GCCACTTCAA AAGAGAAATT GAGTACAGAG AACATTGTTA TTGTTGAATC AAATGCAGAA 
TCAAATCCAG AAGTAGATGC TATGGAATTG AATGTAGAAA ATGATCAGTC TGAAGCTGGC 
TTCTCTGAAC AAAAGAATGT CAAAACTGGT ATTCCAGGGA AAGAAGTAAC AGAGGAAAAC 
AGTGAAAGAA TGGATACACC TGAAAAACTG AAAGCCAATG AAGCAGTAAC TGAGGAATTT 
AATTCAGCTA CTGATTATTC GGATACTCCG ACATCTGTAA AAGTGAATGC TCAGGTAGAG 
ATCTCTGAAC AAATAGCGGT TGGGGAGCTA GGTGGAGGAA GTGATGAATC TGATCCAGGC 
TCATCTGAAG AAATGAACGC TGAAATGAAA AATTGTGAAA AAACAGCGGT TTGTGAGGTG 
ACTACTGAAT CTGACCACGT CACTGAAACC GAAGACGAAG ATGTAACTAC CACGGCTTCT 
CTGCCTATAG AAGTTGAAAC AAAGGAATTA GATGTAGAAG TCAATAACTT TGTTCCCGAC 
ACACTTGAGA TGATCACTGA AGAAGGAAAG GTTGACCCTA ACAAAACTGA AACGAATACT 
GAACCTAATA AATTGGAGGA AACACAGTTA GATGACAATC AGATGGTAGT GGAAAGTAAT 
GAAACTTTAC CGGAAGTTCA CCATACTTCA GAGAAAGTAG AGGAGATAGC ACAACCTCTG 
ACATCTGAAA CAGATATTTC TGGACTAATA TCAGAAGACA GTAATACATC TCCTCAAAAA 
TCGAAGGATA TTGATCCTTT ACTCATGTCA GCAAACGAGA GTCCTAGTGG CATGCACACA 
CGTTGTGTGT GGTCTCCTTT GGCTTCTCCA TCCACCAGCA TTTTAAAGAG AGGACTAAAA 
AGACCCCAAG AAGATGAAAT CTCGTCACCT GTCCACAAGG TTCGCCGTGT CTCCTTTGCA 
GATCCTATAT ACCAAGCAGG ATTGGCAGAT GACATTGATA GGCGATGCTC TATTGTTAGG 
TGCCATTCTT CAAATAGTTC TCTCATGGTA AAAAGTGTTA AAACTTCACC TACTGTGCAA 
TCTAAGCATA ATACCACTTC AGCCAAAGGA TTTCTGTCCC CAGGATCACG TAGCTCTAAA 
TTTAAGAGCT CAAAGAAGTG TTTAATTGCA GAAATGGCTA AGGAATCTGT GCCATGCCCA 
ACAGAAAGTG TTTACCCTGC TTTGGTGAAC TGTGTAGCAC CAGTTGAAAT CATTTTACCT 
CAGATTACAT CCAACATGTG GGCAAGAGGA CTGGGACAGC TCATTAGAGC CAAGAATATA 
AAAACAATTG GCGATTTGAG TACCCTTACA GCATCTGAAA TAAAAACTCT TCCTATCCGT 
TCTCCAAAAG TGTCTAACGT TAAAAAGGCT CTCAGAATAT ATCATGAGCA GCAGGTGAAG 
TCTCGTGGAC TAGAAGAGAT TCCAGTTTTT GATATTTCTG AAAAAACAAT AAATGGAATG 
GAAAATAAAT CTATCTCTCC TGATGAAGAA AGACTTGCCT CAGATTTAAT TGATCCTGTT 
GCTTTAGAAG CTCCGTTATC TAAAAATCTT GTGGCACAGA TTAGCGCACT TGCTCTTCAA 
CTAGATTCAG AAGATCTCTA TAATTATTCA GGAAGCCAGC TATTTGAAAT GCACGAGAAA 
CTTGGTAGCA TGGCAAACTC TATAATTAAA AATCTGCAGT CACGTTGGAG GTCACCAGCC 
CATGAAAATT CTTTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_615269.3
CDS	146..7543
Translation	MTAADQSPLAPLLETLEDPSASLGEQTDAYLTLTSRMTGEDGKEIITEIEKKLPQLFKVLKTHIASQNSELSSAALQALGFCLYNPKIASGLSEINIQELLSKLNDIVKSSDKNVRTRALWVISKQTFPTEVVGKVVSSIIDSLEIVFNRGEMLSAVVDYEALNVIIRLIEQAPVQMGEESIRWAKLVIPLLVHSAQKVHLRGATALEMGMPLLLQKQQEIASITEQLMTTKLLSELQKLFMSKNETYVLKLWPLFVKLLGKTLHRSGSFINSLLQLEELGFRSGAPMIKKIAFIAWKSLIDNFALNPEILCSAKRLKLLMQPLSSIHVRTETLALTKLEVWWYLLMRLGPHLPANFEQVCVPLIQSTISIDSNASPQGSSSRVATTPGLSPMTPVHKGASPYGTPVTPRMNLNSNFGMATIPSIQLLGLEMLLHFLLGPEVVSFAKQNKLILSLEPLEHPLISSSSFFSKHSNTLITAVHDSFVAVGKDASDVVINAIWKELISLVKSVIESGNKKERPGSEVLTLLLKSLESIVNSEVFPVLKTLVLMEITIKGLPQKVLGSPAYQVANMDILNGTPALFLIQLTFNNNLLECGVEDERFFLNLETLVGCVLSGPTSPLAFSDSVLNVVNQNAKQLDNKEHLWRMWSIIVTPLTELINQTNEVNQGDALEHNFSAIYGALILPINHIFPAQKIPVVTMKTLLKTWSELYRAFARCAALVATAEENLCCEEFCSKIMSSLEDEGLSNLLFLDRITHIITVMVDCIDFSPYNIKYQPKTKSPQRPTDWSKKKKEPLGKLASLIKLIVRVIYSFHTLSLKEVHSDTLLTIGNSITSILSNLLGHISLPSMIQKIFAALTRPLALFYENSKLDEVPKVYSCMNNKLEKLLGDIIACLHVSYTGTYDSELLEQISPLLCIIFSHKNKQIRKQSAQFWNATFAKVTMLIYPEELKPILKQAKQKSLLLLPGLENVEIMEESSGPYSDMTENSQLNMKISGMERKSSGKRDSFLAQTKDTKDSMKPPAKLKLDSSTPKAKGEMPLEEEKSTDFVFIPPEVKETKERILTEHQKEVLKTKRCDIPAMYNNLDVSQDTLFSQYSQEESMEISTLIEKSKEDSKMIFKEEQKENDIVVPQHVMENCGMDEHLEKASLLNNECGSIEETSPKILSSNNDARKKALIASNKPTECASTTENPSGVSSSSVSIATISGAPLQPTSRRQSFITLEKFDGSENRPFSPSPLNNMSSTVIVKSNQEGMGKTDNPSKAKKREVALSKSDSEKITHGTKRSSRRSSKSEPIGSKKSKPLMRSEQEKSTQESVDGTVALENNPPGLLNQTECVLDNQVHLSESTVEFEDTMLESTVENTVLENNTVEERTLEVNLESKENTPPAIITDQTVNEDSHIQVTPNQKTLRRSLRRRSETAEPTTDSQDKENNHQKRERRKEEEKTPQKSPLHVKDDVLPKQKLISEQSVQGNLVDKGNNLHEKAFGETSANAEIDENRRKPDLENTKSEGDGVPDIADKSSEKPVRGRTRYQTRRASQGLLSSIENSESDSSEAKEEGSKKKRSGKWKNKSSDNVDMEDQEEKMVKQECIKIENQAHDYKGNPEVDKDIKSQICEKGDESTTVSLQDSTVSPDLLQVYDDVLNTSEGESKSNKDADHSFSNPSVPESNLRTRNASKRLHKRDSIENSMGESSKIGTSDISLLSEKTLQTVECQHKRSRRVRRSKGCDCCGEKSQSQEKSFTGLKDTENYDVKVSETKKTDGETPVSISETSKADLSSEVKLLDEHHSVNFHLDLKEENDTTNDSLIISETELKENTTQSFLPSEMVKFKETSRRDSEEAISLETQEPSNKKCKTVDPHLDVSKDISLKCFSLETKEEKSEATSKEKLSTENIVIVESNAESNPEVDAMELNVENDQSEAGFSEQKNVKTGIPGKEVTEENSERMDTPEKLKANEAVTEEFNSATDYSDTPTSVKVNAQVEISEQIAVGELGGGSDESDPGSSEEMNAEMKNCEKTAVCEVTTESDHVTETEDEDVTTTASLPIEVETKELDVEVNNFVPDTLEMITEEGKVDPNKTETNTEPNKLEETQLDDNQMVVESNETLPEVHHTSEKVEEIAQPLTSETDISGLISEDSNTSPQKSKDIDPLLMSANESPSGMHTRCVWSPLASPSTSILKRGLKRPQEDEISSPVHKVRRVSFADPIYQAGLADDIDRRCSIVRCHSSNSSLMVKSVKTSPTVQSKHNTTSAKGFLSPGSRSSKFKSSKKCLIAEMAKESVPCPTESVYPALVNCVAPVEIILPQITSNMWARGLGQLIRAKNIKTIGDLSTLTASEIKTLPIRSPKVSNVKKALRIYHEQQVKSRGLEEIPVFDISEKTINGMENKSISPDEERLASDLIDPVALEAPLSKNLVAQISALALQLDSEDLYNYSGSQLFEMHEKLGSMANSIIKNLQSRWRSPAHENSF

Target ORF information:

RefSeq Version	XM_615269.9
Organism	Bos taurus(cattle)
Definition	Bos taurus replication timing regulatory factor 1 (RIF1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_615269.9

ORF Insert Sequence:

ATGACGGCCG CGGATCAGAG CCCCCTGGCA CCGCTGTTGG AGACTTTGGA AGACCCTTCC 
GCCTCCCTTG GAGAGCAGAC CGACGCTTAC CTGACCCTGA CCAGTCGTAT GACTGGAGAA 
GATGGAAAAG AAATCATTAC AGAAATTGAG AAAAAACTTC CTCAGCTATT TAAAGTTTTA 
AAGACTCACA TTGCCAGTCA AAACTCAGAG CTCAGTAGTG CTGCTCTACA GGCCTTGGGG 
TTTTGCTTAT ATAATCCCAA AATTGCCTCT GGATTATCAG AGATAAATAT TCAAGAACTG 
CTTTCAAAAC TGAATGATAT TGTTAAAAGT TCAGATAAAA ATGTACGTAC TAGGGCACTT 
TGGGTGATAT CCAAGCAGAC GTTTCCCACT GAAGTCGTTG GCAAAGTAGT TTCTAGTATC 
ATTGACTCAC TAGAAATAGT ATTTAATAGA GGAGAGATGC TTTCTGCTGT TGTGGATTAT 
GAAGCATTAA ATGTCATTAT AAGACTAATT GAACAAGCCC CAGTTCAAAT GGGAGAAGAG 
TCAATTAGGT GGGCAAAATT GGTCATACCT TTACTGGTTC ATTCAGCCCA AAAAGTACAT 
TTGCGGGGAG CCACTGCTCT AGAGATGGGA ATGCCTTTGT TGCTTCAGAA ACAGCAAGAA 
ATAGCATCTA TCACAGAGCA GCTTATGACT ACCAAATTAC TTTCAGAACT CCAGAAACTG 
TTTATGAGTA AAAATGAGAC TTATGTGTTA AAATTGTGGC CTTTATTTGT CAAACTTCTT 
GGGAAGACCT TGCATCGAAG TGGGAGTTTC ATTAATTCTC TGTTACAGCT GGAAGAACTG 
GGATTTCGTA GTGGAGCACC CATGATTAAA AAAATAGCTT TTATTGCTTG GAAGAGTTTA 
ATAGATAATT TTGCTTTAAA TCCAGAAATA CTGTGTAGTG CAAAAAGACT GAAGTTGTTG 
ATGCAGCCTT TGAGTTCTAT CCATGTGAGA ACAGAAACTC TAGCGCTGAC AAAACTAGAA 
GTCTGGTGGT ATTTGTTGAT GAGACTAGGA CCTCATCTTC CTGCTAATTT TGAACAGGTT 
TGTGTGCCTC TGATTCAGAG CACAATAAGC ATTGATTCTA ATGCTTCACC TCAAGGGAGT 
TCATCTCGTG TAGCTACTAC TCCAGGTTTA AGCCCTATGA CCCCAGTACA CAAAGGTGCT 
TCTCCATATG GAACTCCTGT AACCCCTCGG ATGAACTTGA ATTCAAATTT TGGAATGGCC 
ACAATCCCCT CTATACAACT TTTGGGGCTT GAAATGTTGC TTCATTTTTT GTTGGGTCCA 
GAAGTCGTGA GTTTTGCTAA GCAAAACAAA CTTATACTGA GCCTAGAACC ACTTGAACAT 
CCATTAATCA GCAGCTCTTC TTTTTTCTCC AAACACTCAA ATACACTTAT CACTGCTGTT 
CATGATAGCT TTGTTGCAGT TGGAAAAGAT GCTTCTGATG TAGTTATTAA TGCTATTTGG 
AAGGAGTTAA TTAGCTTGGT GAAGTCAGTT ATTGAATCAG GTAACAAAAA AGAGAGACCA 
GGTTCTGAAG TTTTGACTCT CTTATTAAAA TCTTTGGAAA GCATAGTGAA TTCAGAAGTA 
TTTCCTGTAT TGAAAACACT GGTTCTCATG GAAATTACAA TTAAAGGACT TCCTCAGAAA 
GTATTAGGTT CTCCAGCATA TCAGGTTGCT AATATGGATA TTCTTAATGG AACCCCTGCT 
TTGTTCTTAA TTCAGTTAAC TTTCAATAAT AATCTATTGG AATGTGGTGT GGAAGACGAA 
AGGTTTTTTC TCAACCTGGA AACACTTGTG GGCTGTGTTC TTTCGGGTCC AACTTCACCA 
CTCGCTTTCA GTGACTCTGT CTTAAATGTT GTCAATCAAA ATGCAAAGCA GTTGGATAAC 
AAGGAGCATC TTTGGAGAAT GTGGAGTATT ATAGTCACCC CATTAACAGA ATTGATTAAT 
CAGACCAATG AAGTGAATCA AGGGGATGCC TTAGAACATA ATTTCAGTGC TATCTATGGT 
GCACTGATTT TGCCAATAAA CCATATTTTT CCAGCACAAA AAATTCCAGT GGTCACCATG 
AAGACCTTAC TTAAAACTTG GTCAGAATTA TATAGAGCAT TTGCTCGCTG TGCAGCTTTG 
GTGGCAACTG CGGAAGAGAA TTTATGCTGT GAGGAATTTT GTTCCAAGAT AATGTCCAGT 
TTGGAAGATG AAGGCCTTTC AAATTTATTG TTCTTGGATA GGATCACTCA TATTATTACT 
GTAATGGTTG ATTGCATCGA CTTTTCACCA TATAATATTA AATATCAACC CAAAACTAAA 
TCACCACAGA GACCTACAGA TTGGTCCAAA AAGAAGAAGG AACCTTTAGG GAAGTTGGCT 
TCTTTAATTA AACTTATTGT GAGAGTGATC TATTCTTTTC ACACACTGAG CCTCAAGGAA 
GTACATTCTG ATACTCTCCT CACTATTGGC AACTCAATCA CCAGCATTCT TTCCAATTTA 
CTTGGACATA TTTCTCTGCC TTCTATGATC CAGAAAATAT TTGCAGCTTT AACAAGACCT 
CTGGCATTAT TTTATGAAAA CTCAAAGCTT GATGAAGTTC CTAAAGTGTA TAGTTGTATG 
AACAACAAGT TAGAAAAGCT ACTAGGAGAT ATAATTGCTT GTCTACATGT CAGCTACACT 
GGAACTTACG ACAGTGAACT TCTTGAACAG ATCTCACCAT TATTATGCAT AATATTTTCA 
CACAAGAACA AACAGATAAG AAAACAAAGT GCTCAGTTCT GGAATGCCAC ATTTGCTAAA 
GTGACGATGT TGATTTATCC TGAGGAGTTA AAGCCAATAC TGAAACAAGC CAAACAAAAA 
TCTCTGCTTT TATTGCCTGG TTTGGAAAAT GTTGAAATTA TGGAGGAATC CAGTGGACCA 
TATTCAGATA TGACAGAAAA TTCCCAATTA AATATGAAGA TAAGTGGCAT GGAAAGAAAA 
TCAAGTGGAA AAAGAGATTC TTTTTTGGCA CAAACAAAGG ATACAAAAGA CAGTATGAAA 
CCACCAGCTA AATTGAAACT AGATTCTTCA ACTCCAAAAG CAAAGGGTGA AATGCCTTTG 
GAAGAAGAAA AGTCTACTGA CTTTGTGTTT ATACCTCCAG AAGTAAAAGA AACAAAGGAA 
AGAATACTAA CTGAACACCA GAAAGAAGTA CTCAAAACAA AGAGGTGTGA TATTCCTGCC 
ATGTATAATA ATCTGGATGT TTCACAAGAT ACTTTATTTT CTCAGTATAG TCAGGAAGAA 
TCTATGGAAA TTTCTACTTT AATTGAAAAA TCAAAAGAAG ACTCCAAGAT GATATTTAAG 
GAGGAACAAA AGGAGAATGA CATTGTCGTT CCTCAACATG TCATGGAAAA CTGCGGTATG 
GATGAACATC TTGAAAAGGC TTCCCTTTTG AATAATGAGT GTGGTTCTAT TGAGGAAACC 
AGTCCAAAAA TACTGAGCAG TAATAATGAT GCCAGAAAAA AAGCTTTAAT TGCTTCAAAT 
AAACCAACAG AATGTGCATC TACTACAGAA AATCCATCTG GTGTCAGCAG TAGCTCGGTT 
TCTATTGCCA CCATTTCTGG AGCTCCCTTA CAGCCTACAA GTCGAAGGCA ATCCTTTATT 
ACTTTGGAAA AGTTTGATGG TTCAGAAAAT AGACCTTTTA GTCCATCACC CTTGAATAAT 
ATGTCATCAA CTGTAATAGT GAAAAGTAAC CAGGAAGGCA TGGGTAAAAC AGATAATCCA 
TCAAAAGCAA AGAAGAGAGA AGTGGCTCTT TCCAAATCTG ACTCTGAAAA AATAACACAT 
GGGACTAAGA GATCAAGCCG AAGGTCGAGT AAATCTGAAC CAATAGGGAG TAAAAAGTCT 
AAACCCTTAA TGAGATCTGA ACAAGAGAAA AGTACTCAAG AATCTGTTGA TGGCACAGTA 
GCCTTAGAAA ATAACCCACC TGGCTTGCTT AATCAGACAG AATGTGTGTT AGATAATCAG 
GTTCATCTCT CTGAATCTAC AGTGGAGTTT GAGGATACAA TGCTTGAATC AACAGTGGAG 
AATACTGTAT TAGAAAACAA TACTGTAGAA GAGAGAACCT TAGAAGTTAA CTTGGAATCC 
AAAGAAAACA CACCCCCAGC CATAATAACG GATCAGACAG TAAATGAGGA TAGTCATATT 
CAGGTAACTC CAAATCAGAA AACCCTTAGG CGATCTTTAA GGCGACGTTC AGAAACAGCA 
GAACCAACCA CTGATAGCCA AGATAAAGAA AATAATCATC AAAAAAGGGA AAGACGTAAA 
GAAGAAGAAA AGACTCCGCA GAAGAGTCCG TTACATGTAA AAGATGATGT TTTACCTAAG 
CAAAAATTGA TTTCTGAACA AAGTGTACAG GGAAACTTAG TTGACAAAGG AAATAATTTA 
CATGAGAAGG CTTTTGGGGA GACCAGTGCT AATGCTGAAA TTGATGAAAA TAGAAGAAAG 
CCAGATCTTG AGAATACTAA ATCTGAAGGA GATGGTGTGC CGGACATTGC AGATAAATCC 
TCTGAGAAAC CAGTAAGGGG ACGAACACGA TACCAAACAA GAAGAGCATC CCAGGGTTTA 
CTTTCCAGCA TTGAAAACTC AGAATCTGAT AGTTCTGAGG CAAAAGAAGA AGGTTCTAAA 
AAGAAAAGAT CTGGAAAATG GAAGAACAAA AGCAGTGACA ATGTTGACAT GGAAGATCAA 
GAAGAGAAAA TGGTAAAACA GGAATGTATA AAAATTGAAA ATCAGGCACA TGATTATAAA 
GGGAATCCTG AAGTAGACAA AGATATAAAA TCTCAAATCT GTGAAAAAGG AGATGAAAGT 
ACTACTGTAA GTCTTCAAGA TTCTACCGTA TCTCCAGATT TATTACAAGT TTATGATGAT 
GTATTGAATA CTTCTGAGGG AGAAAGTAAA AGTAACAAAG ATGCAGACCA TTCCTTTTCA 
AATCCTTCTG TACCAGAATC AAATCTAAGG ACTAGAAATG CCAGTAAGAG GTTACATAAG 
CGAGATTCTA TAGAAAATAG CATGGGGGAA TCCTCAAAAA TAGGGACATC AGACATTTCT 
TTGCTTTCTG AAAAAACTCT TCAGACAGTA GAATGCCAGC ACAAGAGAAG TAGGAGGGTG 
AGGCGATCTA AAGGTTGTGA TTGTTGTGGA GAAAAATCAC AGTCTCAGGA AAAGTCATTT 
ACTGGGTTAA AGGATACAGA AAATTACGAT GTAAAAGTCA GTGAAACAAA AAAGACAGAT 
GGGGAAACAC CTGTATCTAT TTCAGAAACT TCGAAAGCTG ATCTGTCCTC GGAGGTTAAA 
CTTTTAGATG AGCATCATAG TGTGAATTTT CATTTGGATC TCAAGGAAGA GAATGATACT 
ACTAATGATT CATTAATTAT ATCTGAAACT GAGTTAAAAG AAAATACTAC TCAAAGCTTT 
CTTCCTTCTG AAATGGTAAA ATTTAAAGAA ACTTCTCGTA GGGATTCTGA GGAAGCAATA 
TCTCTAGAAA CCCAGGAGCC ATCTAATAAG AAATGTAAAA CTGTTGACCC ACATTTAGAT 
GTTTCCAAAG ATATTTCACT GAAATGTTTT TCTTTGGAGA CTAAAGAAGA AAAGTCAGAG 
GCCACTTCAA AAGAGAAATT GAGTACAGAG AACATTGTTA TTGTTGAATC AAATGCAGAA 
TCAAATCCAG AAGTAGATGC TATGGAATTG AATGTAGAAA ATGATCAGTC TGAAGCTGGC 
TTCTCTGAAC AAAAGAATGT CAAAACTGGT ATTCCAGGGA AAGAAGTAAC AGAGGAAAAC 
AGTGAAAGAA TGGATACACC TGAAAAACTG AAAGCCAATG AAGCAGTAAC TGAGGAATTT 
AATTCAGCTA CTGATTATTC GGATACTCCG ACATCTGTAA AAGTGAATGC TCAGGTAGAG 
ATCTCTGAAC AAATAGCGGT TGGGGAGCTA GGTGGAGGAA GTGATGAATC TGATCCAGGC 
TCATCTGAAG AAATGAACGC TGAAATGAAA AATTGTGAAA AAACAGCGGT TTGTGAGGTG 
ACTACTGAAT CTGACCACGT CACTGAAACC GAAGACGAAG ATGTAACTAC CACGGCTTCT 
CTGCCTATAG AAGTTGAAAC AAAGGAATTA GATGTAGAAG TCAATAACTT TGTTCCCGAC 
ACACTTGAGA TGATCACTGA AGAAGGAAAG GTTGACCCTA ACAAAACTGA AACGAATACT 
GAACCTAATA AATTGGAGGA AACACAGTTA GATGACAATC AGATGGTAGT GGAAAGTAAT 
GAAACTTTAC CGGAAGTTCA CCATACTTCA GAGAAAGTAG AGGAGATAGC ACAACCTCTG 
ACATCTGAAA CAGATATTTC TGGACTAATA TCAGAAGACA GTAATACATC TCCTCAAAAA 
TCGAAGGATA TTGATCCTTT ACTCATGTCA GCAAACGAGA GTCCTAGTGG CATGCACACA 
CGTTGTGTGT GGTCTCCTTT GGCTTCTCCA TCCACCAGCA TTTTAAAGAG AGGACTAAAA 
AGACCCCAAG AAGATGAAAT CTCGTCACCT GTCCACAAGG TTCGCCGTGT CTCCTTTGCA 
GATCCTATAT ACCAAGCAGG ATTGGCAGAT GACATTGATA GGCGATGCTC TATTGTTAGG 
TGCCATTCTT CAAATAGTTC TCTCATGGTA AAAAGTGTTA AAACTTCACC TACTGTGCAA 
TCTAAGCATA ATACCACTTC AGCCAAAGGA TTTCTGTCCC CAGGATCACG TAGCTCTAAA 
TTTAAGAGCT CAAAGAAGTG TTTAATTGCA GAAATGGCTA AGGAATCTGT GCCATGCCCA 
ACAGAAAGTG TTTACCCTGC TTTGGTGAAC TGTGTAGCAC CAGTTGAAAT CATTTTACCT 
CAGATTACAT CCAACATGTG GGCAAGAGGA CTGGGACAGC TCATTAGAGC CAAGAATATA 
AAAACAATTG GCGATTTGAG TACCCTTACA GCATCTGAAA TAAAAACTCT TCCTATCCGT 
TCTCCAAAAG TGTCTAACGT TAAAAAGGCT CTCAGAATAT ATCATGAGCA GCAGGTGAAG 
TCTCGTGGAC TAGAAGAGAT TCCAGTTTTT GATATTTCTG AAAAAACAAT AAATGGAATG 
GAAAATAAAT CTATCTCTCC TGATGAAGAA AGACTTGCCT CAGATTTAAT TGATCCTGTT 
GCTTTAGAAG CTCCGTTATC TAAAAATCTT GTGGCACAGA TTAGCGCACT TGCTCTTCAA 
CTAGATTCAG AAGATCTCTA TAATTATTCA GGAAGCCAGC TATTTGAAAT GCACGAGAAA 
CTTGGTAGCA TGGCAAACTC TATAATTAAA AATCTGCAGT CACGTTGGAG GTCACCAGCC 
CATGAAAATT CTTTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RIF1 ( XM_010802073.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010800375.1 Bos taurus replication timing regulatory factor 1 (RIF1), transcript variant X2, mRNA.

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CloneID	OBa55065
Clone ID Related Accession (Same CDS sequence)	XM_010802073.3 , XM_010802073.2 , XM_010826489.2
Accession Version	XM_010802073.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7374bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	telomere-associated protein RIF1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010802073.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGCCG CGGATCAGAG CCCCCTGGCA CCGCTGTTGG AGACTTTGGA AGACCCTTCC 
GCCTCCCTTG GAGAGCAGAC CGACGCTTAC CTGACCCTGA CCAGTCGTAT GACTGGAGAA 
GATGGAAAAG AAATCATTAC AGAAATTGAG AAAAAACTTC CTCAGCTATT TAAAGTTTTA 
AAGACTCACA TTGCCAGTCA AAACTCAGAG CTCAGTAGTG CTGCTCTACA GGCCTTGGGG 
TTTTGCTTAT ATAATCCCAA AATTGCCTCT GGATTATCAG AGATAAATAT TCAAGAACTG 
CTTTCAAAAC TGAATGATAT TGTTAAAAGT TCAGATAAAA ATGTACGTAC TAGGGCACTT 
TGGGTGATAT CCAAGCAGAC GTTTCCCACT GAAGTCGTTG GCAAAGTAGT TTCTAGTATC 
ATTGACTCAC TAGAAATAGT ATTTAATAGA GGAGAGATGC TTTCTGCTGT TGTGGATTAT 
GAAGCATTAA ATGTCATTAT AAGACTAATT GAACAAGCCC CAGTTCAAAT GGGAGAAGAG 
TCAATTAGGT GGGCAAAATT GGTCATACCT TTACTGGTTC ATTCAGCCCA AAAAGTACAT 
TTGCGGGGAG CCACTGCTCT AGAGATGGGA ATGCCTTTGT TGCTTCAGAA ACAGCAAGAA 
ATAGCATCTA TCACAGAGCA GCTTATGACT ACCAAATTAC TTTCAGAACT CCAGAAACTG 
TTTATGAGTA AAAATGAGAC TTATGTGTTA AAATTGTGGC CTTTATTTGT CAAACTTCTT 
GGGAAGACCT TGCATCGAAG TGGGAGTTTC ATTAATTCTC TGTTACAGCT GGAAGAACTG 
GGATTTCGTA GTGGAGCACC CATGATTAAA AAAATAGCTT TTATTGCTTG GAAGAGTTTA 
ATAGATAATT TTGCTTTAAA TCCAGAAATA CTGTGTAGTG CAAAAAGACT GAAGTTGTTG 
ATGCAGCCTT TGAGTTCTAT CCATGTGAGA ACAGAAACTC TAGCGCTGAC AAAACTAGAA 
GTCTGGTGGT ATTTGTTGAT GAGACTAGGA CCTCATCTTC CTGCTAATTT TGAACAGGTT 
TGTGTGCCTC TGATTCAGAG CACAATAAGC ATTGATTCTA ATGCTTCACC TCAAGGGAGT 
TCATCTCGTG TAGCTACTAC TCCAGGTTTA AGCCCTATGA CCCCAGTACA CAAAGGTGCT 
TCTCCATATG GAACTCCTGT AACCCCTCGG ATGAACTTGA ATTCAAATTT TGGAATGGCC 
ACAATCCCCT CTATACAACT TTTGGGGCTT GAAATGTTGC TTCATTTTTT GTTGGGTCCA 
GAAGTCGTGA GTTTTGCTAA GCAAAACAAA CTTATACTGA GCCTAGAACC ACTTGAACAT 
CCATTAATCA GCAGCTCTTC TTTTTTCTCC AAACACTCAA ATACACTTAT CACTGCTGTT 
CATGATAGCT TTGTTGCAGT TGGAAAAGAT GCTTCTGATG TAGTTATTAA TGCTATTTGG 
AAGGAGTTAA TTAGCTTGGT GAAGTCAGTT ATTGAATCAG GTAACAAAAA AGAGAGACCA 
GGTTCTGAAG TTTTGACTCT CTTATTAAAA TCTTTGGAAA GCATAGTGAA TTCAGAAGTA 
TTTCCTGTAT TGAAAACACT GGTTCTCATG GAAATTACAA TTAAAGGACT TCCTCAGAAA 
GTATTAGGTT CTCCAGCATA TCAGGGAACC CCTGCTTTGT TCTTAATTCA GTTAACTTTC 
AATAATAATC TATTGGAATG TGGTGTGGAA GACGAAAGGT TTTTTCTCAA CCTGGAAACA 
CTTGTGGGCT GTGTTCTTTC GGGTCCAACT TCACCACTCG CTTTCAGTGA CTCTGTCTTA 
AATGTTGTCA ATCAAAATGC AAAGCAGTTG GATAACAAGG AGCATCTTTG GAGAATGTGG 
AGTATTATAG TCACCCCATT AACAGAATTG ATTAATCAGA CCAATGAAGT GAATCAAGGG 
GATGCCTTAG AACATAATTT CAGTGCTATC TATGGTGCAC TGATTTTGCC AATAAACCAT 
ATTTTTCCAG CACAAAAAAT TCCAGTGGTC ACCATGAAGA CCTTACTTAA AACTTGGTCA 
GAATTATATA GAGCATTTGC TCGCTGTGCA GCTTTGGTGG CAACTGCGGA AGAGAATTTA 
TGCTGTGAGG AATTTTGTTC CAAGATAATG TCCAGTTTGG AAGATGAAGG CCTTTCAAAT 
TTATTGTTCT TGGATAGGAT CACTCATATT ATTACTGTAA TGGTTGATTG CATCGACTTT 
TCACCATATA ATATTAAATA TCAACCCAAA ACTAAATCAC CACAGAGACC TACAGATTGG 
TCCAAAAAGA AGAAGGAACC TTTAGGGAAG TTGGCTTCTT TAATTAAACT TATTGTGAGA 
GTGATCTATT CTTTTCACAC ACTGAGCCTC AAGGAAGTAC ATTCTGATAC TCTCCTCACT 
ATTGGCAACT CAATCACCAG CATTCTTTCC AATTTACTTG GACATATTTC TCTGCCTTCT 
ATGATCCAGA AAATATTTGC AGCTTTAACA AGACCTCTGG CATTATTTTA TGAAAACTCA 
AAGCTTGATG AAGTTCCTAA AGTGTATAGT TGTATGAACA ACAAGTTAGA AAAGCTACTA 
GGAGATATAA TTGCTTGTCT ACATGTCAGC TACACTGGAA CTTACGACAG TGAACTTCTT 
GAACAGATCT CACCATTATT ATGCATAATA TTTTCACACA AGAACAAACA GATAAGAAAA 
CAAAGTGCTC AGTTCTGGAA TGCCACATTT GCTAAAGTGA CGATGTTGAT TTATCCTGAG 
GAGTTAAAGC CAATACTGAA ACAAGCCAAA CAAAAATCTC TGCTTTTATT GCCTGGTTTG 
GAAAATGTTG AAATTATGGA GGAATCCAGT GGACCATATT CAGATATGAC AGAAAATTCC 
CAATTAAATA TGAAGATAAG TGGCATGGAA AGAAAATCAA GTGGAAAAAG AGATTCTTTT 
TTGGCACAAA CAAAGGATAC AAAAGACAGT ATGAAACCAC CAGCTAAATT GAAACTAGAT 
TCTTCAACTC CAAAAGCAAA GGGTGAAATG CCTTTGGAAG AAGAAAAGTC TACTGACTTT 
GTGTTTATAC CTCCAGAAGT AAAAGAAACA AAGGAAAGAA TACTAACTGA ACACCAGAAA 
GAAGTACTCA AAACAAAGAG GTGTGATATT CCTGCCATGT ATAATAATCT GGATGTTTCA 
CAAGATACTT TATTTTCTCA GTATAGTCAG GAAGAATCTA TGGAAATTTC TACTTTAATT 
GAAAAATCAA AAGAAGACTC CAAGATGATA TTTAAGGAGG AACAAAAGGA GAATGACATT 
GTCGTTCCTC AACATGTCAT GGAAAACTGC GGTATGGATG AACATCTTGA AAAGGCTTCC 
CTTTTGAATA ATGAGTGTGG TTCTATTGAG GAAACCAGTC CAAAAATACT GAGCAGTAAT 
AATGATGCCA GAAAAAAAGC TTTAATTGCT TCAAATAAAC CAACAGAATG TGCATCTACT 
ACAGAAAATC CATCTGGTGT CAGCAGTAGC TCGGTTTCTA TTGCCACCAT TTCTGGAGCT 
CCCTTACAGC CTACAAGTCG AAGGCAATCC TTTATTACTT TGGAAAAGTT TGATGGTTCA 
GAAAATAGAC CTTTTAGTCC ATCACCCTTG AATAATATGT CATCAACTGT AATAGTGAAA 
AGTAACCAGG AAGGCATGGG TAAAACAGAT AATCCATCAA AAGCAAAGAA GAGAGAAGTG 
GCTCTTTCCA AATCTGACTC TGAAAAAATA ACACATGGGA CTAAGAGATC AAGCCGAAGG 
TCGAGTAAAT CTGAACCAAT AGGGAGTAAA AAGTCTAAAC CCTTAATGAG ATCTGAACAA 
GAGAAAAGTA CTCAAGAATC TGTTGATGGC ACAGTAGCCT TAGAAAATAA CCCACCTGGC 
TTGCTTAATC AGACAGAATG TGTGTTAGAT AATCAGGTTC ATCTCTCTGA ATCTACAGTG 
GAGTTTGAGG ATACAATGCT TGAATCAACA GTGGAGAATA CTGTATTAGA AAACAATACT 
GTAGAAGAGA GAACCTTAGA AGTTAACTTG GAATCCAAAG AAAACACACC CCCAGCCATA 
ATAACGGATC AGACAGTAAA TGAGGATAGT CATATTCAGG TAACTCCAAA TCAGAAAACC 
CTTAGGCGAT CTTTAAGGCG ACGTTCAGAA ACAGCAGAAC CAACCACTGA TAGCCAAGAT 
AAAGAAAATA ATCATCAAAA AAGGGAAAGA CGTAAAGAAG AAGAAAAGAC TCCGCAGAAG 
AGTCCGTTAC ATGTAAAAGA TGATGTTTTA CCTAAGCAAA AATTGATTTC TGAACAAAGT 
GTACAGGGAA ACTTAGTTGA CAAAGGAAAT AATTTACATG AGAAGGCTTT TGGGGAGACC 
AGTGCTAATG CTGAAATTGA TGAAAATAGA AGAAAGCCAG ATCTTGAGAA TACTAAATCT 
GAAGGAGATG GTGTGCCGGA CATTGCAGAT AAATCCTCTG AGAAACCAGT AAGGGGACGA 
ACACGATACC AAACAAGAAG AGCATCCCAG GGTTTACTTT CCAGCATTGA AAACTCAGAA 
TCTGATAGTT CTGAGGCAAA AGAAGAAGGT TCTAAAAAGA AAAGATCTGG AAAATGGAAG 
AACAAAAGCA GTGACAATGT TGACATGGAA GATCAAGAAG AGAAAATGGT AAAACAGGAA 
TGTATAAAAA TTGAAAATCA GGCACATGAT TATAAAGGGA ATCCTGAAGT AGACAAAGAT 
ATAAAATCTC AAATCTGTGA AAAAGGAGAT GAAAGTACTA CTGTAAGTCT TCAAGATTCT 
ACCGTATCTC CAGATTTATT ACAAGTTTAT GATGATGTAT TGAATACTTC TGAGGGAGAA 
AGTAAAAGTA ACAAAGATGC AGACCATTCC TTTTCAAATC CTTCTGTACC AGAATCAAAT 
CTAAGGACTA GAAATGCCAG TAAGAGGTTA CATAAGCGAG ATTCTATAGA AAATAGCATG 
GGGGAATCCT CAAAAATAGG GACATCAGAC ATTTCTTTGC TTTCTGAAAA AACTCTTCAG 
ACAGTAGAAT GCCAGCACAA GAGAAGTAGG AGGGTGAGGC GATCTAAAGG TTGTGATTGT 
TGTGGAGAAA AATCACAGTC TCAGGAAAAG TCATTTACTG GGTTAAAGGA TACAGAAAAT 
TACGATGTAA AAGTCAGTGA AACAAAAAAG ACAGATGGGG AAACACCTGT ATCTATTTCA 
GAAACTTCGA AAGCTGATCT GTCCTCGGAG GTTAAACTTT TAGATGAGCA TCATAGTGTG 
AATTTTCATT TGGATCTCAA GGAAGAGAAT GATACTACTA ATGATTCATT AATTATATCT 
GAAACTGAGT TAAAAGAAAA TACTACTCAA AGCTTTCTTC CTTCTGAAAT GGTAAAATTT 
AAAGAAACTT CTCGTAGGGA TTCTGAGGAA GCAATATCTC TAGAAACCCA GGAGCCATCT 
AATAAGAAAT GTAAAACTGT TGACCCACAT TTAGATGTTT CCAAAGATAT TTCACTGAAA 
TGTTTTTCTT TGGAGACTAA AGAAGAAAAG TCAGAGGCCA CTTCAAAAGA GAAATTGAGT 
ACAGAGAACA TTGTTATTGT TGAATCAAAT GCAGAATCAA ATCCAGAAGT AGATGCTATG 
GAATTGAATG TAGAAAATGA TCAGTCTGAA GCTGGCTTCT CTGAACAAAA GAATGTCAAA 
ACTGGTATTC CAGGGAAAGA AGTAACAGAG GAAAACAGTG AAAGAATGGA TACACCTGAA 
AAACTGAAAG CCAATGAAGC AGTAACTGAG GAATTTAATT CAGCTACTGA TTATTCGGAT 
ACTCCGACAT CTGTAAAAGT GAATGCTCAG GTAGAGATCT CTGAACAAAT AGCGGTTGGG 
GAGCTAGGTG GAGGAAGTGA TGAATCTGAT CCAGGCTCAT CTGAAGAAAT GAACGCTGAA 
ATGAAAAATT GTGAAAAAAC AGCGGTTTGT GAGGTGACTA CTGAATCTGA CCACGTCACT 
GAAACCGAAG ACGAAGATGT AACTACCACG GCTTCTCTGC CTATAGAAGT TGAAACAAAG 
GAATTAGATG TAGAAGTCAA TAACTTTGTT CCCGACACAC TTGAGATGAT CACTGAAGAA 
GGAAAGGTTG ACCCTAACAA AACTGAAACG AATACTGAAC CTAATAAATT GGAGGAAACA 
CAGTTAGATG ACAATCAGAT GGTAGTGGAA AGTAATGAAA CTTTACCGGA AGTTCACCAT 
ACTTCAGAGA AAGTAGAGGA GATAGCACAA CCTCTGACAT CTGAAACAGA TATTTCTGGA 
CTAATATCAG AAGACAGTAA TACATCTCCT CAAAAATCGA AGGATATTGA TCCTTTACTC 
ATGTCAGCAA ACGAGAGTCC TAGTGGCATG CACACACGTT GTGTGTGGTC TCCTTTGGCT 
TCTCCATCCA CCAGCATTTT AAAGAGAGGA CTAAAAAGAC CCCAAGAAGA TGAAATCTCG 
TCACCTGTCC ACAAGGTTCG CCGTGTCTCC TTTGCAGATC CTATATACCA AGCAGGATTG 
GCAGATGACA TTGATAGGCG ATGCTCTATT GTTAGGTGCC ATTCTTCAAA TAGTTCTCTC 
ATGGTAAAAA GTGTTAAAAC TTCACCTACT GTGCAATCTA AGCATAATAC CACTTCAGCC 
AAAGGATTTC TGTCCCCAGG ATCACGTAGC TCTAAATTTA AGAGCTCAAA GAAGTGTTTA 
ATTGCAGAAA TGGCTAAGGA ATCTGTGCCA TGCCCAACAG AAAGTGTTTA CCCTGCTTTG 
GTGAACTGTG TAGCACCAGT TGAAATCATT TTACCTCAGA TTACATCCAA CATGTGGGCA 
AGAGGACTGG GACAGCTCAT TAGAGCCAAG AATATAAAAA CAATTGGCGA TTTGAGTACC 
CTTACAGCAT CTGAAATAAA AACTCTTCCT ATCCGTTCTC CAAAAGTGTC TAACGTTAAA 
AAGGCTCTCA GAATATATCA TGAGCAGCAG GTGAAGTCTC GTGGACTAGA AGAGATTCCA 
GTTTTTGATA TTTCTGAAAA AACAATAAAT GGAATGGAAA ATAAATCTAT CTCTCCTGAT 
GAAGAAAGAC TTGCCTCAGA TTTAATTGAT CCTGTTGCTT TAGAAGCTCC GTTATCTAAA 
AATCTTGTGG CACAGATTAG CGCACTTGCT CTTCAACTAG ATTCAGAAGA TCTCTATAAT 
TATTCAGGAA GCCAGCTATT TGAAATGCAC GAGAAACTTG GTAGCATGGC AAACTCTATA 
ATTAAAAATC TGCAGTCACG TTGGAGGTCA CCAGCCCATG AAAATTCTTT TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010800375.1
CDS	171..7544
Translation	MTAADQSPLAPLLETLEDPSASLGEQTDAYLTLTSRMTGEDGKEIITEIEKKLPQLFKVLKTHIASQNSELSSAALQALGFCLYNPKIASGLSEINIQELLSKLNDIVKSSDKNVRTRALWVISKQTFPTEVVGKVVSSIIDSLEIVFNRGEMLSAVVDYEALNVIIRLIEQAPVQMGEESIRWAKLVIPLLVHSAQKVHLRGATALEMGMPLLLQKQQEIASITEQLMTTKLLSELQKLFMSKNETYVLKLWPLFVKLLGKTLHRSGSFINSLLQLEELGFRSGAPMIKKIAFIAWKSLIDNFALNPEILCSAKRLKLLMQPLSSIHVRTETLALTKLEVWWYLLMRLGPHLPANFEQVCVPLIQSTISIDSNASPQGSSSRVATTPGLSPMTPVHKGASPYGTPVTPRMNLNSNFGMATIPSIQLLGLEMLLHFLLGPEVVSFAKQNKLILSLEPLEHPLISSSSFFSKHSNTLITAVHDSFVAVGKDASDVVINAIWKELISLVKSVIESGNKKERPGSEVLTLLLKSLESIVNSEVFPVLKTLVLMEITIKGLPQKVLGSPAYQGTPALFLIQLTFNNNLLECGVEDERFFLNLETLVGCVLSGPTSPLAFSDSVLNVVNQNAKQLDNKEHLWRMWSIIVTPLTELINQTNEVNQGDALEHNFSAIYGALILPINHIFPAQKIPVVTMKTLLKTWSELYRAFARCAALVATAEENLCCEEFCSKIMSSLEDEGLSNLLFLDRITHIITVMVDCIDFSPYNIKYQPKTKSPQRPTDWSKKKKEPLGKLASLIKLIVRVIYSFHTLSLKEVHSDTLLTIGNSITSILSNLLGHISLPSMIQKIFAALTRPLALFYENSKLDEVPKVYSCMNNKLEKLLGDIIACLHVSYTGTYDSELLEQISPLLCIIFSHKNKQIRKQSAQFWNATFAKVTMLIYPEELKPILKQAKQKSLLLLPGLENVEIMEESSGPYSDMTENSQLNMKISGMERKSSGKRDSFLAQTKDTKDSMKPPAKLKLDSSTPKAKGEMPLEEEKSTDFVFIPPEVKETKERILTEHQKEVLKTKRCDIPAMYNNLDVSQDTLFSQYSQEESMEISTLIEKSKEDSKMIFKEEQKENDIVVPQHVMENCGMDEHLEKASLLNNECGSIEETSPKILSSNNDARKKALIASNKPTECASTTENPSGVSSSSVSIATISGAPLQPTSRRQSFITLEKFDGSENRPFSPSPLNNMSSTVIVKSNQEGMGKTDNPSKAKKREVALSKSDSEKITHGTKRSSRRSSKSEPIGSKKSKPLMRSEQEKSTQESVDGTVALENNPPGLLNQTECVLDNQVHLSESTVEFEDTMLESTVENTVLENNTVEERTLEVNLESKENTPPAIITDQTVNEDSHIQVTPNQKTLRRSLRRRSETAEPTTDSQDKENNHQKRERRKEEEKTPQKSPLHVKDDVLPKQKLISEQSVQGNLVDKGNNLHEKAFGETSANAEIDENRRKPDLENTKSEGDGVPDIADKSSEKPVRGRTRYQTRRASQGLLSSIENSESDSSEAKEEGSKKKRSGKWKNKSSDNVDMEDQEEKMVKQECIKIENQAHDYKGNPEVDKDIKSQICEKGDESTTVSLQDSTVSPDLLQVYDDVLNTSEGESKSNKDADHSFSNPSVPESNLRTRNASKRLHKRDSIENSMGESSKIGTSDISLLSEKTLQTVECQHKRSRRVRRSKGCDCCGEKSQSQEKSFTGLKDTENYDVKVSETKKTDGETPVSISETSKADLSSEVKLLDEHHSVNFHLDLKEENDTTNDSLIISETELKENTTQSFLPSEMVKFKETSRRDSEEAISLETQEPSNKKCKTVDPHLDVSKDISLKCFSLETKEEKSEATSKEKLSTENIVIVESNAESNPEVDAMELNVENDQSEAGFSEQKNVKTGIPGKEVTEENSERMDTPEKLKANEAVTEEFNSATDYSDTPTSVKVNAQVEISEQIAVGELGGGSDESDPGSSEEMNAEMKNCEKTAVCEVTTESDHVTETEDEDVTTTASLPIEVETKELDVEVNNFVPDTLEMITEEGKVDPNKTETNTEPNKLEETQLDDNQMVVESNETLPEVHHTSEKVEEIAQPLTSETDISGLISEDSNTSPQKSKDIDPLLMSANESPSGMHTRCVWSPLASPSTSILKRGLKRPQEDEISSPVHKVRRVSFADPIYQAGLADDIDRRCSIVRCHSSNSSLMVKSVKTSPTVQSKHNTTSAKGFLSPGSRSSKFKSSKKCLIAEMAKESVPCPTESVYPALVNCVAPVEIILPQITSNMWARGLGQLIRAKNIKTIGDLSTLTASEIKTLPIRSPKVSNVKKALRIYHEQQVKSRGLEEIPVFDISEKTINGMENKSISPDEERLASDLIDPVALEAPLSKNLVAQISALALQLDSEDLYNYSGSQLFEMHEKLGSMANSIIKNLQSRWRSPAHENSF

Target ORF information:

RefSeq Version	XM_010802073.3
Organism	Bos taurus(cattle)
Definition	Bos taurus replication timing regulatory factor 1 (RIF1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010802073.3

ORF Insert Sequence:

ATGACGGCCG CGGATCAGAG CCCCCTGGCA CCGCTGTTGG AGACTTTGGA AGACCCTTCC 
GCCTCCCTTG GAGAGCAGAC CGACGCTTAC CTGACCCTGA CCAGTCGTAT GACTGGAGAA 
GATGGAAAAG AAATCATTAC AGAAATTGAG AAAAAACTTC CTCAGCTATT TAAAGTTTTA 
AAGACTCACA TTGCCAGTCA AAACTCAGAG CTCAGTAGTG CTGCTCTACA GGCCTTGGGG 
TTTTGCTTAT ATAATCCCAA AATTGCCTCT GGATTATCAG AGATAAATAT TCAAGAACTG 
CTTTCAAAAC TGAATGATAT TGTTAAAAGT TCAGATAAAA ATGTACGTAC TAGGGCACTT 
TGGGTGATAT CCAAGCAGAC GTTTCCCACT GAAGTCGTTG GCAAAGTAGT TTCTAGTATC 
ATTGACTCAC TAGAAATAGT ATTTAATAGA GGAGAGATGC TTTCTGCTGT TGTGGATTAT 
GAAGCATTAA ATGTCATTAT AAGACTAATT GAACAAGCCC CAGTTCAAAT GGGAGAAGAG 
TCAATTAGGT GGGCAAAATT GGTCATACCT TTACTGGTTC ATTCAGCCCA AAAAGTACAT 
TTGCGGGGAG CCACTGCTCT AGAGATGGGA ATGCCTTTGT TGCTTCAGAA ACAGCAAGAA 
ATAGCATCTA TCACAGAGCA GCTTATGACT ACCAAATTAC TTTCAGAACT CCAGAAACTG 
TTTATGAGTA AAAATGAGAC TTATGTGTTA AAATTGTGGC CTTTATTTGT CAAACTTCTT 
GGGAAGACCT TGCATCGAAG TGGGAGTTTC ATTAATTCTC TGTTACAGCT GGAAGAACTG 
GGATTTCGTA GTGGAGCACC CATGATTAAA AAAATAGCTT TTATTGCTTG GAAGAGTTTA 
ATAGATAATT TTGCTTTAAA TCCAGAAATA CTGTGTAGTG CAAAAAGACT GAAGTTGTTG 
ATGCAGCCTT TGAGTTCTAT CCATGTGAGA ACAGAAACTC TAGCGCTGAC AAAACTAGAA 
GTCTGGTGGT ATTTGTTGAT GAGACTAGGA CCTCATCTTC CTGCTAATTT TGAACAGGTT 
TGTGTGCCTC TGATTCAGAG CACAATAAGC ATTGATTCTA ATGCTTCACC TCAAGGGAGT 
TCATCTCGTG TAGCTACTAC TCCAGGTTTA AGCCCTATGA CCCCAGTACA CAAAGGTGCT 
TCTCCATATG GAACTCCTGT AACCCCTCGG ATGAACTTGA ATTCAAATTT TGGAATGGCC 
ACAATCCCCT CTATACAACT TTTGGGGCTT GAAATGTTGC TTCATTTTTT GTTGGGTCCA 
GAAGTCGTGA GTTTTGCTAA GCAAAACAAA CTTATACTGA GCCTAGAACC ACTTGAACAT 
CCATTAATCA GCAGCTCTTC TTTTTTCTCC AAACACTCAA ATACACTTAT CACTGCTGTT 
CATGATAGCT TTGTTGCAGT TGGAAAAGAT GCTTCTGATG TAGTTATTAA TGCTATTTGG 
AAGGAGTTAA TTAGCTTGGT GAAGTCAGTT ATTGAATCAG GTAACAAAAA AGAGAGACCA 
GGTTCTGAAG TTTTGACTCT CTTATTAAAA TCTTTGGAAA GCATAGTGAA TTCAGAAGTA 
TTTCCTGTAT TGAAAACACT GGTTCTCATG GAAATTACAA TTAAAGGACT TCCTCAGAAA 
GTATTAGGTT CTCCAGCATA TCAGGGAACC CCTGCTTTGT TCTTAATTCA GTTAACTTTC 
AATAATAATC TATTGGAATG TGGTGTGGAA GACGAAAGGT TTTTTCTCAA CCTGGAAACA 
CTTGTGGGCT GTGTTCTTTC GGGTCCAACT TCACCACTCG CTTTCAGTGA CTCTGTCTTA 
AATGTTGTCA ATCAAAATGC AAAGCAGTTG GATAACAAGG AGCATCTTTG GAGAATGTGG 
AGTATTATAG TCACCCCATT AACAGAATTG ATTAATCAGA CCAATGAAGT GAATCAAGGG 
GATGCCTTAG AACATAATTT CAGTGCTATC TATGGTGCAC TGATTTTGCC AATAAACCAT 
ATTTTTCCAG CACAAAAAAT TCCAGTGGTC ACCATGAAGA CCTTACTTAA AACTTGGTCA 
GAATTATATA GAGCATTTGC TCGCTGTGCA GCTTTGGTGG CAACTGCGGA AGAGAATTTA 
TGCTGTGAGG AATTTTGTTC CAAGATAATG TCCAGTTTGG AAGATGAAGG CCTTTCAAAT 
TTATTGTTCT TGGATAGGAT CACTCATATT ATTACTGTAA TGGTTGATTG CATCGACTTT 
TCACCATATA ATATTAAATA TCAACCCAAA ACTAAATCAC CACAGAGACC TACAGATTGG 
TCCAAAAAGA AGAAGGAACC TTTAGGGAAG TTGGCTTCTT TAATTAAACT TATTGTGAGA 
GTGATCTATT CTTTTCACAC ACTGAGCCTC AAGGAAGTAC ATTCTGATAC TCTCCTCACT 
ATTGGCAACT CAATCACCAG CATTCTTTCC AATTTACTTG GACATATTTC TCTGCCTTCT 
ATGATCCAGA AAATATTTGC AGCTTTAACA AGACCTCTGG CATTATTTTA TGAAAACTCA 
AAGCTTGATG AAGTTCCTAA AGTGTATAGT TGTATGAACA ACAAGTTAGA AAAGCTACTA 
GGAGATATAA TTGCTTGTCT ACATGTCAGC TACACTGGAA CTTACGACAG TGAACTTCTT 
GAACAGATCT CACCATTATT ATGCATAATA TTTTCACACA AGAACAAACA GATAAGAAAA 
CAAAGTGCTC AGTTCTGGAA TGCCACATTT GCTAAAGTGA CGATGTTGAT TTATCCTGAG 
GAGTTAAAGC CAATACTGAA ACAAGCCAAA CAAAAATCTC TGCTTTTATT GCCTGGTTTG 
GAAAATGTTG AAATTATGGA GGAATCCAGT GGACCATATT CAGATATGAC AGAAAATTCC 
CAATTAAATA TGAAGATAAG TGGCATGGAA AGAAAATCAA GTGGAAAAAG AGATTCTTTT 
TTGGCACAAA CAAAGGATAC AAAAGACAGT ATGAAACCAC CAGCTAAATT GAAACTAGAT 
TCTTCAACTC CAAAAGCAAA GGGTGAAATG CCTTTGGAAG AAGAAAAGTC TACTGACTTT 
GTGTTTATAC CTCCAGAAGT AAAAGAAACA AAGGAAAGAA TACTAACTGA ACACCAGAAA 
GAAGTACTCA AAACAAAGAG GTGTGATATT CCTGCCATGT ATAATAATCT GGATGTTTCA 
CAAGATACTT TATTTTCTCA GTATAGTCAG GAAGAATCTA TGGAAATTTC TACTTTAATT 
GAAAAATCAA AAGAAGACTC CAAGATGATA TTTAAGGAGG AACAAAAGGA GAATGACATT 
GTCGTTCCTC AACATGTCAT GGAAAACTGC GGTATGGATG AACATCTTGA AAAGGCTTCC 
CTTTTGAATA ATGAGTGTGG TTCTATTGAG GAAACCAGTC CAAAAATACT GAGCAGTAAT 
AATGATGCCA GAAAAAAAGC TTTAATTGCT TCAAATAAAC CAACAGAATG TGCATCTACT 
ACAGAAAATC CATCTGGTGT CAGCAGTAGC TCGGTTTCTA TTGCCACCAT TTCTGGAGCT 
CCCTTACAGC CTACAAGTCG AAGGCAATCC TTTATTACTT TGGAAAAGTT TGATGGTTCA 
GAAAATAGAC CTTTTAGTCC ATCACCCTTG AATAATATGT CATCAACTGT AATAGTGAAA 
AGTAACCAGG AAGGCATGGG TAAAACAGAT AATCCATCAA AAGCAAAGAA GAGAGAAGTG 
GCTCTTTCCA AATCTGACTC TGAAAAAATA ACACATGGGA CTAAGAGATC AAGCCGAAGG 
TCGAGTAAAT CTGAACCAAT AGGGAGTAAA AAGTCTAAAC CCTTAATGAG ATCTGAACAA 
GAGAAAAGTA CTCAAGAATC TGTTGATGGC ACAGTAGCCT TAGAAAATAA CCCACCTGGC 
TTGCTTAATC AGACAGAATG TGTGTTAGAT AATCAGGTTC ATCTCTCTGA ATCTACAGTG 
GAGTTTGAGG ATACAATGCT TGAATCAACA GTGGAGAATA CTGTATTAGA AAACAATACT 
GTAGAAGAGA GAACCTTAGA AGTTAACTTG GAATCCAAAG AAAACACACC CCCAGCCATA 
ATAACGGATC AGACAGTAAA TGAGGATAGT CATATTCAGG TAACTCCAAA TCAGAAAACC 
CTTAGGCGAT CTTTAAGGCG ACGTTCAGAA ACAGCAGAAC CAACCACTGA TAGCCAAGAT 
AAAGAAAATA ATCATCAAAA AAGGGAAAGA CGTAAAGAAG AAGAAAAGAC TCCGCAGAAG 
AGTCCGTTAC ATGTAAAAGA TGATGTTTTA CCTAAGCAAA AATTGATTTC TGAACAAAGT 
GTACAGGGAA ACTTAGTTGA CAAAGGAAAT AATTTACATG AGAAGGCTTT TGGGGAGACC 
AGTGCTAATG CTGAAATTGA TGAAAATAGA AGAAAGCCAG ATCTTGAGAA TACTAAATCT 
GAAGGAGATG GTGTGCCGGA CATTGCAGAT AAATCCTCTG AGAAACCAGT AAGGGGACGA 
ACACGATACC AAACAAGAAG AGCATCCCAG GGTTTACTTT CCAGCATTGA AAACTCAGAA 
TCTGATAGTT CTGAGGCAAA AGAAGAAGGT TCTAAAAAGA AAAGATCTGG AAAATGGAAG 
AACAAAAGCA GTGACAATGT TGACATGGAA GATCAAGAAG AGAAAATGGT AAAACAGGAA 
TGTATAAAAA TTGAAAATCA GGCACATGAT TATAAAGGGA ATCCTGAAGT AGACAAAGAT 
ATAAAATCTC AAATCTGTGA AAAAGGAGAT GAAAGTACTA CTGTAAGTCT TCAAGATTCT 
ACCGTATCTC CAGATTTATT ACAAGTTTAT GATGATGTAT TGAATACTTC TGAGGGAGAA 
AGTAAAAGTA ACAAAGATGC AGACCATTCC TTTTCAAATC CTTCTGTACC AGAATCAAAT 
CTAAGGACTA GAAATGCCAG TAAGAGGTTA CATAAGCGAG ATTCTATAGA AAATAGCATG 
GGGGAATCCT CAAAAATAGG GACATCAGAC ATTTCTTTGC TTTCTGAAAA AACTCTTCAG 
ACAGTAGAAT GCCAGCACAA GAGAAGTAGG AGGGTGAGGC GATCTAAAGG TTGTGATTGT 
TGTGGAGAAA AATCACAGTC TCAGGAAAAG TCATTTACTG GGTTAAAGGA TACAGAAAAT 
TACGATGTAA AAGTCAGTGA AACAAAAAAG ACAGATGGGG AAACACCTGT ATCTATTTCA 
GAAACTTCGA AAGCTGATCT GTCCTCGGAG GTTAAACTTT TAGATGAGCA TCATAGTGTG 
AATTTTCATT TGGATCTCAA GGAAGAGAAT GATACTACTA ATGATTCATT AATTATATCT 
GAAACTGAGT TAAAAGAAAA TACTACTCAA AGCTTTCTTC CTTCTGAAAT GGTAAAATTT 
AAAGAAACTT CTCGTAGGGA TTCTGAGGAA GCAATATCTC TAGAAACCCA GGAGCCATCT 
AATAAGAAAT GTAAAACTGT TGACCCACAT TTAGATGTTT CCAAAGATAT TTCACTGAAA 
TGTTTTTCTT TGGAGACTAA AGAAGAAAAG TCAGAGGCCA CTTCAAAAGA GAAATTGAGT 
ACAGAGAACA TTGTTATTGT TGAATCAAAT GCAGAATCAA ATCCAGAAGT AGATGCTATG 
GAATTGAATG TAGAAAATGA TCAGTCTGAA GCTGGCTTCT CTGAACAAAA GAATGTCAAA 
ACTGGTATTC CAGGGAAAGA AGTAACAGAG GAAAACAGTG AAAGAATGGA TACACCTGAA 
AAACTGAAAG CCAATGAAGC AGTAACTGAG GAATTTAATT CAGCTACTGA TTATTCGGAT 
ACTCCGACAT CTGTAAAAGT GAATGCTCAG GTAGAGATCT CTGAACAAAT AGCGGTTGGG 
GAGCTAGGTG GAGGAAGTGA TGAATCTGAT CCAGGCTCAT CTGAAGAAAT GAACGCTGAA 
ATGAAAAATT GTGAAAAAAC AGCGGTTTGT GAGGTGACTA CTGAATCTGA CCACGTCACT 
GAAACCGAAG ACGAAGATGT AACTACCACG GCTTCTCTGC CTATAGAAGT TGAAACAAAG 
GAATTAGATG TAGAAGTCAA TAACTTTGTT CCCGACACAC TTGAGATGAT CACTGAAGAA 
GGAAAGGTTG ACCCTAACAA AACTGAAACG AATACTGAAC CTAATAAATT GGAGGAAACA 
CAGTTAGATG ACAATCAGAT GGTAGTGGAA AGTAATGAAA CTTTACCGGA AGTTCACCAT 
ACTTCAGAGA AAGTAGAGGA GATAGCACAA CCTCTGACAT CTGAAACAGA TATTTCTGGA 
CTAATATCAG AAGACAGTAA TACATCTCCT CAAAAATCGA AGGATATTGA TCCTTTACTC 
ATGTCAGCAA ACGAGAGTCC TAGTGGCATG CACACACGTT GTGTGTGGTC TCCTTTGGCT 
TCTCCATCCA CCAGCATTTT AAAGAGAGGA CTAAAAAGAC CCCAAGAAGA TGAAATCTCG 
TCACCTGTCC ACAAGGTTCG CCGTGTCTCC TTTGCAGATC CTATATACCA AGCAGGATTG 
GCAGATGACA TTGATAGGCG ATGCTCTATT GTTAGGTGCC ATTCTTCAAA TAGTTCTCTC 
ATGGTAAAAA GTGTTAAAAC TTCACCTACT GTGCAATCTA AGCATAATAC CACTTCAGCC 
AAAGGATTTC TGTCCCCAGG ATCACGTAGC TCTAAATTTA AGAGCTCAAA GAAGTGTTTA 
ATTGCAGAAA TGGCTAAGGA ATCTGTGCCA TGCCCAACAG AAAGTGTTTA CCCTGCTTTG 
GTGAACTGTG TAGCACCAGT TGAAATCATT TTACCTCAGA TTACATCCAA CATGTGGGCA 
AGAGGACTGG GACAGCTCAT TAGAGCCAAG AATATAAAAA CAATTGGCGA TTTGAGTACC 
CTTACAGCAT CTGAAATAAA AACTCTTCCT ATCCGTTCTC CAAAAGTGTC TAACGTTAAA 
AAGGCTCTCA GAATATATCA TGAGCAGCAG GTGAAGTCTC GTGGACTAGA AGAGATTCCA 
GTTTTTGATA TTTCTGAAAA AACAATAAAT GGAATGGAAA ATAAATCTAT CTCTCCTGAT 
GAAGAAAGAC TTGCCTCAGA TTTAATTGAT CCTGTTGCTT TAGAAGCTCC GTTATCTAAA 
AATCTTGTGG CACAGATTAG CGCACTTGCT CTTCAACTAG ATTCAGAAGA TCTCTATAAT 
TATTCAGGAA GCCAGCTATT TGAAATGCAC GAGAAACTTG GTAGCATGGC AAACTCTATA 
ATTAAAAATC TGCAGTCACG TTGGAGGTCA CCAGCCCATG AAAATTCTTT TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RIF1 ( XM_010802073.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010800375.1 Bos taurus replication timing regulatory factor 1 (RIF1), transcript variant X2, mRNA.

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CloneID	OBa55065
Clone ID Related Accession (Same CDS sequence)	XM_010802073.3 , XM_010802073.2 , XM_010826489.2
Accession Version	XM_010802073.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7374bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	telomere-associated protein RIF1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103838.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010802073.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGCCG CGGATCAGAG CCCCCTGGCA CCGCTGTTGG AGACTTTGGA AGACCCTTCC 
GCCTCCCTTG GAGAGCAGAC CGACGCTTAC CTGACCCTGA CCAGTCGTAT GACTGGAGAA 
GATGGAAAAG AAATCATTAC AGAAATTGAG AAAAAACTTC CTCAGCTATT TAAAGTTTTA 
AAGACTCACA TTGCCAGTCA AAACTCAGAG CTCAGTAGTG CTGCTCTACA GGCCTTGGGG 
TTTTGCTTAT ATAATCCCAA AATTGCCTCT GGATTATCAG AGATAAATAT TCAAGAACTG 
CTTTCAAAAC TGAATGATAT TGTTAAAAGT TCAGATAAAA ATGTACGTAC TAGGGCACTT 
TGGGTGATAT CCAAGCAGAC GTTTCCCACT GAAGTCGTTG GCAAAGTAGT TTCTAGTATC 
ATTGACTCAC TAGAAATAGT ATTTAATAGA GGAGAGATGC TTTCTGCTGT TGTGGATTAT 
GAAGCATTAA ATGTCATTAT AAGACTAATT GAACAAGCCC CAGTTCAAAT GGGAGAAGAG 
TCAATTAGGT GGGCAAAATT GGTCATACCT TTACTGGTTC ATTCAGCCCA AAAAGTACAT 
TTGCGGGGAG CCACTGCTCT AGAGATGGGA ATGCCTTTGT TGCTTCAGAA ACAGCAAGAA 
ATAGCATCTA TCACAGAGCA GCTTATGACT ACCAAATTAC TTTCAGAACT CCAGAAACTG 
TTTATGAGTA AAAATGAGAC TTATGTGTTA AAATTGTGGC CTTTATTTGT CAAACTTCTT 
GGGAAGACCT TGCATCGAAG TGGGAGTTTC ATTAATTCTC TGTTACAGCT GGAAGAACTG 
GGATTTCGTA GTGGAGCACC CATGATTAAA AAAATAGCTT TTATTGCTTG GAAGAGTTTA 
ATAGATAATT TTGCTTTAAA TCCAGAAATA CTGTGTAGTG CAAAAAGACT GAAGTTGTTG 
ATGCAGCCTT TGAGTTCTAT CCATGTGAGA ACAGAAACTC TAGCGCTGAC AAAACTAGAA 
GTCTGGTGGT ATTTGTTGAT GAGACTAGGA CCTCATCTTC CTGCTAATTT TGAACAGGTT 
TGTGTGCCTC TGATTCAGAG CACAATAAGC ATTGATTCTA ATGCTTCACC TCAAGGGAGT 
TCATCTCGTG TAGCTACTAC TCCAGGTTTA AGCCCTATGA CCCCAGTACA CAAAGGTGCT 
TCTCCATATG GAACTCCTGT AACCCCTCGG ATGAACTTGA ATTCAAATTT TGGAATGGCC 
ACAATCCCCT CTATACAACT TTTGGGGCTT GAAATGTTGC TTCATTTTTT GTTGGGTCCA 
GAAGTCGTGA GTTTTGCTAA GCAAAACAAA CTTATACTGA GCCTAGAACC ACTTGAACAT 
CCATTAATCA GCAGCTCTTC TTTTTTCTCC AAACACTCAA ATACACTTAT CACTGCTGTT 
CATGATAGCT TTGTTGCAGT TGGAAAAGAT GCTTCTGATG TAGTTATTAA TGCTATTTGG 
AAGGAGTTAA TTAGCTTGGT GAAGTCAGTT ATTGAATCAG GTAACAAAAA AGAGAGACCA 
GGTTCTGAAG TTTTGACTCT CTTATTAAAA TCTTTGGAAA GCATAGTGAA TTCAGAAGTA 
TTTCCTGTAT TGAAAACACT GGTTCTCATG GAAATTACAA TTAAAGGACT TCCTCAGAAA 
GTATTAGGTT CTCCAGCATA TCAGGGAACC CCTGCTTTGT TCTTAATTCA GTTAACTTTC 
AATAATAATC TATTGGAATG TGGTGTGGAA GACGAAAGGT TTTTTCTCAA CCTGGAAACA 
CTTGTGGGCT GTGTTCTTTC GGGTCCAACT TCACCACTCG CTTTCAGTGA CTCTGTCTTA 
AATGTTGTCA ATCAAAATGC AAAGCAGTTG GATAACAAGG AGCATCTTTG GAGAATGTGG 
AGTATTATAG TCACCCCATT AACAGAATTG ATTAATCAGA CCAATGAAGT GAATCAAGGG 
GATGCCTTAG AACATAATTT CAGTGCTATC TATGGTGCAC TGATTTTGCC AATAAACCAT 
ATTTTTCCAG CACAAAAAAT TCCAGTGGTC ACCATGAAGA CCTTACTTAA AACTTGGTCA 
GAATTATATA GAGCATTTGC TCGCTGTGCA GCTTTGGTGG CAACTGCGGA AGAGAATTTA 
TGCTGTGAGG AATTTTGTTC CAAGATAATG TCCAGTTTGG AAGATGAAGG CCTTTCAAAT 
TTATTGTTCT TGGATAGGAT CACTCATATT ATTACTGTAA TGGTTGATTG CATCGACTTT 
TCACCATATA ATATTAAATA TCAACCCAAA ACTAAATCAC CACAGAGACC TACAGATTGG 
TCCAAAAAGA AGAAGGAACC TTTAGGGAAG TTGGCTTCTT TAATTAAACT TATTGTGAGA 
GTGATCTATT CTTTTCACAC ACTGAGCCTC AAGGAAGTAC ATTCTGATAC TCTCCTCACT 
ATTGGCAACT CAATCACCAG CATTCTTTCC AATTTACTTG GACATATTTC TCTGCCTTCT 
ATGATCCAGA AAATATTTGC AGCTTTAACA AGACCTCTGG CATTATTTTA TGAAAACTCA 
AAGCTTGATG AAGTTCCTAA AGTGTATAGT TGTATGAACA ACAAGTTAGA AAAGCTACTA 
GGAGATATAA TTGCTTGTCT ACATGTCAGC TACACTGGAA CTTACGACAG TGAACTTCTT 
GAACAGATCT CACCATTATT ATGCATAATA TTTTCACACA AGAACAAACA GATAAGAAAA 
CAAAGTGCTC AGTTCTGGAA TGCCACATTT GCTAAAGTGA CGATGTTGAT TTATCCTGAG 
GAGTTAAAGC CAATACTGAA ACAAGCCAAA CAAAAATCTC TGCTTTTATT GCCTGGTTTG 
GAAAATGTTG AAATTATGGA GGAATCCAGT GGACCATATT CAGATATGAC AGAAAATTCC 
CAATTAAATA TGAAGATAAG TGGCATGGAA AGAAAATCAA GTGGAAAAAG AGATTCTTTT 
TTGGCACAAA CAAAGGATAC AAAAGACAGT ATGAAACCAC CAGCTAAATT GAAACTAGAT 
TCTTCAACTC CAAAAGCAAA GGGTGAAATG CCTTTGGAAG AAGAAAAGTC TACTGACTTT 
GTGTTTATAC CTCCAGAAGT AAAAGAAACA AAGGAAAGAA TACTAACTGA ACACCAGAAA 
GAAGTACTCA AAACAAAGAG GTGTGATATT CCTGCCATGT ATAATAATCT GGATGTTTCA 
CAAGATACTT TATTTTCTCA GTATAGTCAG GAAGAATCTA TGGAAATTTC TACTTTAATT 
GAAAAATCAA AAGAAGACTC CAAGATGATA TTTAAGGAGG AACAAAAGGA GAATGACATT 
GTCGTTCCTC AACATGTCAT GGAAAACTGC GGTATGGATG AACATCTTGA AAAGGCTTCC 
CTTTTGAATA ATGAGTGTGG TTCTATTGAG GAAACCAGTC CAAAAATACT GAGCAGTAAT 
AATGATGCCA GAAAAAAAGC TTTAATTGCT TCAAATAAAC CAACAGAATG TGCATCTACT 
ACAGAAAATC CATCTGGTGT CAGCAGTAGC TCGGTTTCTA TTGCCACCAT TTCTGGAGCT 
CCCTTACAGC CTACAAGTCG AAGGCAATCC TTTATTACTT TGGAAAAGTT TGATGGTTCA 
GAAAATAGAC CTTTTAGTCC ATCACCCTTG AATAATATGT CATCAACTGT AATAGTGAAA 
AGTAACCAGG AAGGCATGGG TAAAACAGAT AATCCATCAA AAGCAAAGAA GAGAGAAGTG 
GCTCTTTCCA AATCTGACTC TGAAAAAATA ACACATGGGA CTAAGAGATC AAGCCGAAGG 
TCGAGTAAAT CTGAACCAAT AGGGAGTAAA AAGTCTAAAC CCTTAATGAG ATCTGAACAA 
GAGAAAAGTA CTCAAGAATC TGTTGATGGC ACAGTAGCCT TAGAAAATAA CCCACCTGGC 
TTGCTTAATC AGACAGAATG TGTGTTAGAT AATCAGGTTC ATCTCTCTGA ATCTACAGTG 
GAGTTTGAGG ATACAATGCT TGAATCAACA GTGGAGAATA CTGTATTAGA AAACAATACT 
GTAGAAGAGA GAACCTTAGA AGTTAACTTG GAATCCAAAG AAAACACACC CCCAGCCATA 
ATAACGGATC AGACAGTAAA TGAGGATAGT CATATTCAGG TAACTCCAAA TCAGAAAACC 
CTTAGGCGAT CTTTAAGGCG ACGTTCAGAA ACAGCAGAAC CAACCACTGA TAGCCAAGAT 
AAAGAAAATA ATCATCAAAA AAGGGAAAGA CGTAAAGAAG AAGAAAAGAC TCCGCAGAAG 
AGTCCGTTAC ATGTAAAAGA TGATGTTTTA CCTAAGCAAA AATTGATTTC TGAACAAAGT 
GTACAGGGAA ACTTAGTTGA CAAAGGAAAT AATTTACATG AGAAGGCTTT TGGGGAGACC 
AGTGCTAATG CTGAAATTGA TGAAAATAGA AGAAAGCCAG ATCTTGAGAA TACTAAATCT 
GAAGGAGATG GTGTGCCGGA CATTGCAGAT AAATCCTCTG AGAAACCAGT AAGGGGACGA 
ACACGATACC AAACAAGAAG AGCATCCCAG GGTTTACTTT CCAGCATTGA AAACTCAGAA 
TCTGATAGTT CTGAGGCAAA AGAAGAAGGT TCTAAAAAGA AAAGATCTGG AAAATGGAAG 
AACAAAAGCA GTGACAATGT TGACATGGAA GATCAAGAAG AGAAAATGGT AAAACAGGAA 
TGTATAAAAA TTGAAAATCA GGCACATGAT TATAAAGGGA ATCCTGAAGT AGACAAAGAT 
ATAAAATCTC AAATCTGTGA AAAAGGAGAT GAAAGTACTA CTGTAAGTCT TCAAGATTCT 
ACCGTATCTC CAGATTTATT ACAAGTTTAT GATGATGTAT TGAATACTTC TGAGGGAGAA 
AGTAAAAGTA ACAAAGATGC AGACCATTCC TTTTCAAATC CTTCTGTACC AGAATCAAAT 
CTAAGGACTA GAAATGCCAG TAAGAGGTTA CATAAGCGAG ATTCTATAGA AAATAGCATG 
GGGGAATCCT CAAAAATAGG GACATCAGAC ATTTCTTTGC TTTCTGAAAA AACTCTTCAG 
ACAGTAGAAT GCCAGCACAA GAGAAGTAGG AGGGTGAGGC GATCTAAAGG TTGTGATTGT 
TGTGGAGAAA AATCACAGTC TCAGGAAAAG TCATTTACTG GGTTAAAGGA TACAGAAAAT 
TACGATGTAA AAGTCAGTGA AACAAAAAAG ACAGATGGGG AAACACCTGT ATCTATTTCA 
GAAACTTCGA AAGCTGATCT GTCCTCGGAG GTTAAACTTT TAGATGAGCA TCATAGTGTG 
AATTTTCATT TGGATCTCAA GGAAGAGAAT GATACTACTA ATGATTCATT AATTATATCT 
GAAACTGAGT TAAAAGAAAA TACTACTCAA AGCTTTCTTC CTTCTGAAAT GGTAAAATTT 
AAAGAAACTT CTCGTAGGGA TTCTGAGGAA GCAATATCTC TAGAAACCCA GGAGCCATCT 
AATAAGAAAT GTAAAACTGT TGACCCACAT TTAGATGTTT CCAAAGATAT TTCACTGAAA 
TGTTTTTCTT TGGAGACTAA AGAAGAAAAG TCAGAGGCCA CTTCAAAAGA GAAATTGAGT 
ACAGAGAACA TTGTTATTGT TGAATCAAAT GCAGAATCAA ATCCAGAAGT AGATGCTATG 
GAATTGAATG TAGAAAATGA TCAGTCTGAA GCTGGCTTCT CTGAACAAAA GAATGTCAAA 
ACTGGTATTC CAGGGAAAGA AGTAACAGAG GAAAACAGTG AAAGAATGGA TACACCTGAA 
AAACTGAAAG CCAATGAAGC AGTAACTGAG GAATTTAATT CAGCTACTGA TTATTCGGAT 
ACTCCGACAT CTGTAAAAGT GAATGCTCAG GTAGAGATCT CTGAACAAAT AGCGGTTGGG 
GAGCTAGGTG GAGGAAGTGA TGAATCTGAT CCAGGCTCAT CTGAAGAAAT GAACGCTGAA 
ATGAAAAATT GTGAAAAAAC AGCGGTTTGT GAGGTGACTA CTGAATCTGA CCACGTCACT 
GAAACCGAAG ACGAAGATGT AACTACCACG GCTTCTCTGC CTATAGAAGT TGAAACAAAG 
GAATTAGATG TAGAAGTCAA TAACTTTGTT CCCGACACAC TTGAGATGAT CACTGAAGAA 
GGAAAGGTTG ACCCTAACAA AACTGAAACG AATACTGAAC CTAATAAATT GGAGGAAACA 
CAGTTAGATG ACAATCAGAT GGTAGTGGAA AGTAATGAAA CTTTACCGGA AGTTCACCAT 
ACTTCAGAGA AAGTAGAGGA GATAGCACAA CCTCTGACAT CTGAAACAGA TATTTCTGGA 
CTAATATCAG AAGACAGTAA TACATCTCCT CAAAAATCGA AGGATATTGA TCCTTTACTC 
ATGTCAGCAA ACGAGAGTCC TAGTGGCATG CACACACGTT GTGTGTGGTC TCCTTTGGCT 
TCTCCATCCA CCAGCATTTT AAAGAGAGGA CTAAAAAGAC CCCAAGAAGA TGAAATCTCG 
TCACCTGTCC ACAAGGTTCG CCGTGTCTCC TTTGCAGATC CTATATACCA AGCAGGATTG 
GCAGATGACA TTGATAGGCG ATGCTCTATT GTTAGGTGCC ATTCTTCAAA TAGTTCTCTC 
ATGGTAAAAA GTGTTAAAAC TTCACCTACT GTGCAATCTA AGCATAATAC CACTTCAGCC 
AAAGGATTTC TGTCCCCAGG ATCACGTAGC TCTAAATTTA AGAGCTCAAA GAAGTGTTTA 
ATTGCAGAAA TGGCTAAGGA ATCTGTGCCA TGCCCAACAG AAAGTGTTTA CCCTGCTTTG 
GTGAACTGTG TAGCACCAGT TGAAATCATT TTACCTCAGA TTACATCCAA CATGTGGGCA 
AGAGGACTGG GACAGCTCAT TAGAGCCAAG AATATAAAAA CAATTGGCGA TTTGAGTACC 
CTTACAGCAT CTGAAATAAA AACTCTTCCT ATCCGTTCTC CAAAAGTGTC TAACGTTAAA 
AAGGCTCTCA GAATATATCA TGAGCAGCAG GTGAAGTCTC GTGGACTAGA AGAGATTCCA 
GTTTTTGATA TTTCTGAAAA AACAATAAAT GGAATGGAAA ATAAATCTAT CTCTCCTGAT 
GAAGAAAGAC TTGCCTCAGA TTTAATTGAT CCTGTTGCTT TAGAAGCTCC GTTATCTAAA 
AATCTTGTGG CACAGATTAG CGCACTTGCT CTTCAACTAG ATTCAGAAGA TCTCTATAAT 
TATTCAGGAA GCCAGCTATT TGAAATGCAC GAGAAACTTG GTAGCATGGC AAACTCTATA 
ATTAAAAATC TGCAGTCACG TTGGAGGTCA CCAGCCCATG AAAATTCTTT TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010800375.1
CDS	146..7519
Translation	MTAADQSPLAPLLETLEDPSASLGEQTDAYLTLTSRMTGEDGKEIITEIEKKLPQLFKVLKTHIASQNSELSSAALQALGFCLYNPKIASGLSEINIQELLSKLNDIVKSSDKNVRTRALWVISKQTFPTEVVGKVVSSIIDSLEIVFNRGEMLSAVVDYEALNVIIRLIEQAPVQMGEESIRWAKLVIPLLVHSAQKVHLRGATALEMGMPLLLQKQQEIASITEQLMTTKLLSELQKLFMSKNETYVLKLWPLFVKLLGKTLHRSGSFINSLLQLEELGFRSGAPMIKKIAFIAWKSLIDNFALNPEILCSAKRLKLLMQPLSSIHVRTETLALTKLEVWWYLLMRLGPHLPANFEQVCVPLIQSTISIDSNASPQGSSSRVATTPGLSPMTPVHKGASPYGTPVTPRMNLNSNFGMATIPSIQLLGLEMLLHFLLGPEVVSFAKQNKLILSLEPLEHPLISSSSFFSKHSNTLITAVHDSFVAVGKDASDVVINAIWKELISLVKSVIESGNKKERPGSEVLTLLLKSLESIVNSEVFPVLKTLVLMEITIKGLPQKVLGSPAYQGTPALFLIQLTFNNNLLECGVEDERFFLNLETLVGCVLSGPTSPLAFSDSVLNVVNQNAKQLDNKEHLWRMWSIIVTPLTELINQTNEVNQGDALEHNFSAIYGALILPINHIFPAQKIPVVTMKTLLKTWSELYRAFARCAALVATAEENLCCEEFCSKIMSSLEDEGLSNLLFLDRITHIITVMVDCIDFSPYNIKYQPKTKSPQRPTDWSKKKKEPLGKLASLIKLIVRVIYSFHTLSLKEVHSDTLLTIGNSITSILSNLLGHISLPSMIQKIFAALTRPLALFYENSKLDEVPKVYSCMNNKLEKLLGDIIACLHVSYTGTYDSELLEQISPLLCIIFSHKNKQIRKQSAQFWNATFAKVTMLIYPEELKPILKQAKQKSLLLLPGLENVEIMEESSGPYSDMTENSQLNMKISGMERKSSGKRDSFLAQTKDTKDSMKPPAKLKLDSSTPKAKGEMPLEEEKSTDFVFIPPEVKETKERILTEHQKEVLKTKRCDIPAMYNNLDVSQDTLFSQYSQEESMEISTLIEKSKEDSKMIFKEEQKENDIVVPQHVMENCGMDEHLEKASLLNNECGSIEETSPKILSSNNDARKKALIASNKPTECASTTENPSGVSSSSVSIATISGAPLQPTSRRQSFITLEKFDGSENRPFSPSPLNNMSSTVIVKSNQEGMGKTDNPSKAKKREVALSKSDSEKITHGTKRSSRRSSKSEPIGSKKSKPLMRSEQEKSTQESVDGTVALENNPPGLLNQTECVLDNQVHLSESTVEFEDTMLESTVENTVLENNTVEERTLEVNLESKENTPPAIITDQTVNEDSHIQVTPNQKTLRRSLRRRSETAEPTTDSQDKENNHQKRERRKEEEKTPQKSPLHVKDDVLPKQKLISEQSVQGNLVDKGNNLHEKAFGETSANAEIDENRRKPDLENTKSEGDGVPDIADKSSEKPVRGRTRYQTRRASQGLLSSIENSESDSSEAKEEGSKKKRSGKWKNKSSDNVDMEDQEEKMVKQECIKIENQAHDYKGNPEVDKDIKSQICEKGDESTTVSLQDSTVSPDLLQVYDDVLNTSEGESKSNKDADHSFSNPSVPESNLRTRNASKRLHKRDSIENSMGESSKIGTSDISLLSEKTLQTVECQHKRSRRVRRSKGCDCCGEKSQSQEKSFTGLKDTENYDVKVSETKKTDGETPVSISETSKADLSSEVKLLDEHHSVNFHLDLKEENDTTNDSLIISETELKENTTQSFLPSEMVKFKETSRRDSEEAISLETQEPSNKKCKTVDPHLDVSKDISLKCFSLETKEEKSEATSKEKLSTENIVIVESNAESNPEVDAMELNVENDQSEAGFSEQKNVKTGIPGKEVTEENSERMDTPEKLKANEAVTEEFNSATDYSDTPTSVKVNAQVEISEQIAVGELGGGSDESDPGSSEEMNAEMKNCEKTAVCEVTTESDHVTETEDEDVTTTASLPIEVETKELDVEVNNFVPDTLEMITEEGKVDPNKTETNTEPNKLEETQLDDNQMVVESNETLPEVHHTSEKVEEIAQPLTSETDISGLISEDSNTSPQKSKDIDPLLMSANESPSGMHTRCVWSPLASPSTSILKRGLKRPQEDEISSPVHKVRRVSFADPIYQAGLADDIDRRCSIVRCHSSNSSLMVKSVKTSPTVQSKHNTTSAKGFLSPGSRSSKFKSSKKCLIAEMAKESVPCPTESVYPALVNCVAPVEIILPQITSNMWARGLGQLIRAKNIKTIGDLSTLTASEIKTLPIRSPKVSNVKKALRIYHEQQVKSRGLEEIPVFDISEKTINGMENKSISPDEERLASDLIDPVALEAPLSKNLVAQISALALQLDSEDLYNYSGSQLFEMHEKLGSMANSIIKNLQSRWRSPAHENSF

Target ORF information:

RefSeq Version	XM_010802073.2
Organism	Bos taurus(cattle)
Definition	Bos taurus replication timing regulatory factor 1 (RIF1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010802073.2

ORF Insert Sequence:

ATGACGGCCG CGGATCAGAG CCCCCTGGCA CCGCTGTTGG AGACTTTGGA AGACCCTTCC 
GCCTCCCTTG GAGAGCAGAC CGACGCTTAC CTGACCCTGA CCAGTCGTAT GACTGGAGAA 
GATGGAAAAG AAATCATTAC AGAAATTGAG AAAAAACTTC CTCAGCTATT TAAAGTTTTA 
AAGACTCACA TTGCCAGTCA AAACTCAGAG CTCAGTAGTG CTGCTCTACA GGCCTTGGGG 
TTTTGCTTAT ATAATCCCAA AATTGCCTCT GGATTATCAG AGATAAATAT TCAAGAACTG 
CTTTCAAAAC TGAATGATAT TGTTAAAAGT TCAGATAAAA ATGTACGTAC TAGGGCACTT 
TGGGTGATAT CCAAGCAGAC GTTTCCCACT GAAGTCGTTG GCAAAGTAGT TTCTAGTATC 
ATTGACTCAC TAGAAATAGT ATTTAATAGA GGAGAGATGC TTTCTGCTGT TGTGGATTAT 
GAAGCATTAA ATGTCATTAT AAGACTAATT GAACAAGCCC CAGTTCAAAT GGGAGAAGAG 
TCAATTAGGT GGGCAAAATT GGTCATACCT TTACTGGTTC ATTCAGCCCA AAAAGTACAT 
TTGCGGGGAG CCACTGCTCT AGAGATGGGA ATGCCTTTGT TGCTTCAGAA ACAGCAAGAA 
ATAGCATCTA TCACAGAGCA GCTTATGACT ACCAAATTAC TTTCAGAACT CCAGAAACTG 
TTTATGAGTA AAAATGAGAC TTATGTGTTA AAATTGTGGC CTTTATTTGT CAAACTTCTT 
GGGAAGACCT TGCATCGAAG TGGGAGTTTC ATTAATTCTC TGTTACAGCT GGAAGAACTG 
GGATTTCGTA GTGGAGCACC CATGATTAAA AAAATAGCTT TTATTGCTTG GAAGAGTTTA 
ATAGATAATT TTGCTTTAAA TCCAGAAATA CTGTGTAGTG CAAAAAGACT GAAGTTGTTG 
ATGCAGCCTT TGAGTTCTAT CCATGTGAGA ACAGAAACTC TAGCGCTGAC AAAACTAGAA 
GTCTGGTGGT ATTTGTTGAT GAGACTAGGA CCTCATCTTC CTGCTAATTT TGAACAGGTT 
TGTGTGCCTC TGATTCAGAG CACAATAAGC ATTGATTCTA ATGCTTCACC TCAAGGGAGT 
TCATCTCGTG TAGCTACTAC TCCAGGTTTA AGCCCTATGA CCCCAGTACA CAAAGGTGCT 
TCTCCATATG GAACTCCTGT AACCCCTCGG ATGAACTTGA ATTCAAATTT TGGAATGGCC 
ACAATCCCCT CTATACAACT TTTGGGGCTT GAAATGTTGC TTCATTTTTT GTTGGGTCCA 
GAAGTCGTGA GTTTTGCTAA GCAAAACAAA CTTATACTGA GCCTAGAACC ACTTGAACAT 
CCATTAATCA GCAGCTCTTC TTTTTTCTCC AAACACTCAA ATACACTTAT CACTGCTGTT 
CATGATAGCT TTGTTGCAGT TGGAAAAGAT GCTTCTGATG TAGTTATTAA TGCTATTTGG 
AAGGAGTTAA TTAGCTTGGT GAAGTCAGTT ATTGAATCAG GTAACAAAAA AGAGAGACCA 
GGTTCTGAAG TTTTGACTCT CTTATTAAAA TCTTTGGAAA GCATAGTGAA TTCAGAAGTA 
TTTCCTGTAT TGAAAACACT GGTTCTCATG GAAATTACAA TTAAAGGACT TCCTCAGAAA 
GTATTAGGTT CTCCAGCATA TCAGGGAACC CCTGCTTTGT TCTTAATTCA GTTAACTTTC 
AATAATAATC TATTGGAATG TGGTGTGGAA GACGAAAGGT TTTTTCTCAA CCTGGAAACA 
CTTGTGGGCT GTGTTCTTTC GGGTCCAACT TCACCACTCG CTTTCAGTGA CTCTGTCTTA 
AATGTTGTCA ATCAAAATGC AAAGCAGTTG GATAACAAGG AGCATCTTTG GAGAATGTGG 
AGTATTATAG TCACCCCATT AACAGAATTG ATTAATCAGA CCAATGAAGT GAATCAAGGG 
GATGCCTTAG AACATAATTT CAGTGCTATC TATGGTGCAC TGATTTTGCC AATAAACCAT 
ATTTTTCCAG CACAAAAAAT TCCAGTGGTC ACCATGAAGA CCTTACTTAA AACTTGGTCA 
GAATTATATA GAGCATTTGC TCGCTGTGCA GCTTTGGTGG CAACTGCGGA AGAGAATTTA 
TGCTGTGAGG AATTTTGTTC CAAGATAATG TCCAGTTTGG AAGATGAAGG CCTTTCAAAT 
TTATTGTTCT TGGATAGGAT CACTCATATT ATTACTGTAA TGGTTGATTG CATCGACTTT 
TCACCATATA ATATTAAATA TCAACCCAAA ACTAAATCAC CACAGAGACC TACAGATTGG 
TCCAAAAAGA AGAAGGAACC TTTAGGGAAG TTGGCTTCTT TAATTAAACT TATTGTGAGA 
GTGATCTATT CTTTTCACAC ACTGAGCCTC AAGGAAGTAC ATTCTGATAC TCTCCTCACT 
ATTGGCAACT CAATCACCAG CATTCTTTCC AATTTACTTG GACATATTTC TCTGCCTTCT 
ATGATCCAGA AAATATTTGC AGCTTTAACA AGACCTCTGG CATTATTTTA TGAAAACTCA 
AAGCTTGATG AAGTTCCTAA AGTGTATAGT TGTATGAACA ACAAGTTAGA AAAGCTACTA 
GGAGATATAA TTGCTTGTCT ACATGTCAGC TACACTGGAA CTTACGACAG TGAACTTCTT 
GAACAGATCT CACCATTATT ATGCATAATA TTTTCACACA AGAACAAACA GATAAGAAAA 
CAAAGTGCTC AGTTCTGGAA TGCCACATTT GCTAAAGTGA CGATGTTGAT TTATCCTGAG 
GAGTTAAAGC CAATACTGAA ACAAGCCAAA CAAAAATCTC TGCTTTTATT GCCTGGTTTG 
GAAAATGTTG AAATTATGGA GGAATCCAGT GGACCATATT CAGATATGAC AGAAAATTCC 
CAATTAAATA TGAAGATAAG TGGCATGGAA AGAAAATCAA GTGGAAAAAG AGATTCTTTT 
TTGGCACAAA CAAAGGATAC AAAAGACAGT ATGAAACCAC CAGCTAAATT GAAACTAGAT 
TCTTCAACTC CAAAAGCAAA GGGTGAAATG CCTTTGGAAG AAGAAAAGTC TACTGACTTT 
GTGTTTATAC CTCCAGAAGT AAAAGAAACA AAGGAAAGAA TACTAACTGA ACACCAGAAA 
GAAGTACTCA AAACAAAGAG GTGTGATATT CCTGCCATGT ATAATAATCT GGATGTTTCA 
CAAGATACTT TATTTTCTCA GTATAGTCAG GAAGAATCTA TGGAAATTTC TACTTTAATT 
GAAAAATCAA AAGAAGACTC CAAGATGATA TTTAAGGAGG AACAAAAGGA GAATGACATT 
GTCGTTCCTC AACATGTCAT GGAAAACTGC GGTATGGATG AACATCTTGA AAAGGCTTCC 
CTTTTGAATA ATGAGTGTGG TTCTATTGAG GAAACCAGTC CAAAAATACT GAGCAGTAAT 
AATGATGCCA GAAAAAAAGC TTTAATTGCT TCAAATAAAC CAACAGAATG TGCATCTACT 
ACAGAAAATC CATCTGGTGT CAGCAGTAGC TCGGTTTCTA TTGCCACCAT TTCTGGAGCT 
CCCTTACAGC CTACAAGTCG AAGGCAATCC TTTATTACTT TGGAAAAGTT TGATGGTTCA 
GAAAATAGAC CTTTTAGTCC ATCACCCTTG AATAATATGT CATCAACTGT AATAGTGAAA 
AGTAACCAGG AAGGCATGGG TAAAACAGAT AATCCATCAA AAGCAAAGAA GAGAGAAGTG 
GCTCTTTCCA AATCTGACTC TGAAAAAATA ACACATGGGA CTAAGAGATC AAGCCGAAGG 
TCGAGTAAAT CTGAACCAAT AGGGAGTAAA AAGTCTAAAC CCTTAATGAG ATCTGAACAA 
GAGAAAAGTA CTCAAGAATC TGTTGATGGC ACAGTAGCCT TAGAAAATAA CCCACCTGGC 
TTGCTTAATC AGACAGAATG TGTGTTAGAT AATCAGGTTC ATCTCTCTGA ATCTACAGTG 
GAGTTTGAGG ATACAATGCT TGAATCAACA GTGGAGAATA CTGTATTAGA AAACAATACT 
GTAGAAGAGA GAACCTTAGA AGTTAACTTG GAATCCAAAG AAAACACACC CCCAGCCATA 
ATAACGGATC AGACAGTAAA TGAGGATAGT CATATTCAGG TAACTCCAAA TCAGAAAACC 
CTTAGGCGAT CTTTAAGGCG ACGTTCAGAA ACAGCAGAAC CAACCACTGA TAGCCAAGAT 
AAAGAAAATA ATCATCAAAA AAGGGAAAGA CGTAAAGAAG AAGAAAAGAC TCCGCAGAAG 
AGTCCGTTAC ATGTAAAAGA TGATGTTTTA CCTAAGCAAA AATTGATTTC TGAACAAAGT 
GTACAGGGAA ACTTAGTTGA CAAAGGAAAT AATTTACATG AGAAGGCTTT TGGGGAGACC 
AGTGCTAATG CTGAAATTGA TGAAAATAGA AGAAAGCCAG ATCTTGAGAA TACTAAATCT 
GAAGGAGATG GTGTGCCGGA CATTGCAGAT AAATCCTCTG AGAAACCAGT AAGGGGACGA 
ACACGATACC AAACAAGAAG AGCATCCCAG GGTTTACTTT CCAGCATTGA AAACTCAGAA 
TCTGATAGTT CTGAGGCAAA AGAAGAAGGT TCTAAAAAGA AAAGATCTGG AAAATGGAAG 
AACAAAAGCA GTGACAATGT TGACATGGAA GATCAAGAAG AGAAAATGGT AAAACAGGAA 
TGTATAAAAA TTGAAAATCA GGCACATGAT TATAAAGGGA ATCCTGAAGT AGACAAAGAT 
ATAAAATCTC AAATCTGTGA AAAAGGAGAT GAAAGTACTA CTGTAAGTCT TCAAGATTCT 
ACCGTATCTC CAGATTTATT ACAAGTTTAT GATGATGTAT TGAATACTTC TGAGGGAGAA 
AGTAAAAGTA ACAAAGATGC AGACCATTCC TTTTCAAATC CTTCTGTACC AGAATCAAAT 
CTAAGGACTA GAAATGCCAG TAAGAGGTTA CATAAGCGAG ATTCTATAGA AAATAGCATG 
GGGGAATCCT CAAAAATAGG GACATCAGAC ATTTCTTTGC TTTCTGAAAA AACTCTTCAG 
ACAGTAGAAT GCCAGCACAA GAGAAGTAGG AGGGTGAGGC GATCTAAAGG TTGTGATTGT 
TGTGGAGAAA AATCACAGTC TCAGGAAAAG TCATTTACTG GGTTAAAGGA TACAGAAAAT 
TACGATGTAA AAGTCAGTGA AACAAAAAAG ACAGATGGGG AAACACCTGT ATCTATTTCA 
GAAACTTCGA AAGCTGATCT GTCCTCGGAG GTTAAACTTT TAGATGAGCA TCATAGTGTG 
AATTTTCATT TGGATCTCAA GGAAGAGAAT GATACTACTA ATGATTCATT AATTATATCT 
GAAACTGAGT TAAAAGAAAA TACTACTCAA AGCTTTCTTC CTTCTGAAAT GGTAAAATTT 
AAAGAAACTT CTCGTAGGGA TTCTGAGGAA GCAATATCTC TAGAAACCCA GGAGCCATCT 
AATAAGAAAT GTAAAACTGT TGACCCACAT TTAGATGTTT CCAAAGATAT TTCACTGAAA 
TGTTTTTCTT TGGAGACTAA AGAAGAAAAG TCAGAGGCCA CTTCAAAAGA GAAATTGAGT 
ACAGAGAACA TTGTTATTGT TGAATCAAAT GCAGAATCAA ATCCAGAAGT AGATGCTATG 
GAATTGAATG TAGAAAATGA TCAGTCTGAA GCTGGCTTCT CTGAACAAAA GAATGTCAAA 
ACTGGTATTC CAGGGAAAGA AGTAACAGAG GAAAACAGTG AAAGAATGGA TACACCTGAA 
AAACTGAAAG CCAATGAAGC AGTAACTGAG GAATTTAATT CAGCTACTGA TTATTCGGAT 
ACTCCGACAT CTGTAAAAGT GAATGCTCAG GTAGAGATCT CTGAACAAAT AGCGGTTGGG 
GAGCTAGGTG GAGGAAGTGA TGAATCTGAT CCAGGCTCAT CTGAAGAAAT GAACGCTGAA 
ATGAAAAATT GTGAAAAAAC AGCGGTTTGT GAGGTGACTA CTGAATCTGA CCACGTCACT 
GAAACCGAAG ACGAAGATGT AACTACCACG GCTTCTCTGC CTATAGAAGT TGAAACAAAG 
GAATTAGATG TAGAAGTCAA TAACTTTGTT CCCGACACAC TTGAGATGAT CACTGAAGAA 
GGAAAGGTTG ACCCTAACAA AACTGAAACG AATACTGAAC CTAATAAATT GGAGGAAACA 
CAGTTAGATG ACAATCAGAT GGTAGTGGAA AGTAATGAAA CTTTACCGGA AGTTCACCAT 
ACTTCAGAGA AAGTAGAGGA GATAGCACAA CCTCTGACAT CTGAAACAGA TATTTCTGGA 
CTAATATCAG AAGACAGTAA TACATCTCCT CAAAAATCGA AGGATATTGA TCCTTTACTC 
ATGTCAGCAA ACGAGAGTCC TAGTGGCATG CACACACGTT GTGTGTGGTC TCCTTTGGCT 
TCTCCATCCA CCAGCATTTT AAAGAGAGGA CTAAAAAGAC CCCAAGAAGA TGAAATCTCG 
TCACCTGTCC ACAAGGTTCG CCGTGTCTCC TTTGCAGATC CTATATACCA AGCAGGATTG 
GCAGATGACA TTGATAGGCG ATGCTCTATT GTTAGGTGCC ATTCTTCAAA TAGTTCTCTC 
ATGGTAAAAA GTGTTAAAAC TTCACCTACT GTGCAATCTA AGCATAATAC CACTTCAGCC 
AAAGGATTTC TGTCCCCAGG ATCACGTAGC TCTAAATTTA AGAGCTCAAA GAAGTGTTTA 
ATTGCAGAAA TGGCTAAGGA ATCTGTGCCA TGCCCAACAG AAAGTGTTTA CCCTGCTTTG 
GTGAACTGTG TAGCACCAGT TGAAATCATT TTACCTCAGA TTACATCCAA CATGTGGGCA 
AGAGGACTGG GACAGCTCAT TAGAGCCAAG AATATAAAAA CAATTGGCGA TTTGAGTACC 
CTTACAGCAT CTGAAATAAA AACTCTTCCT ATCCGTTCTC CAAAAGTGTC TAACGTTAAA 
AAGGCTCTCA GAATATATCA TGAGCAGCAG GTGAAGTCTC GTGGACTAGA AGAGATTCCA 
GTTTTTGATA TTTCTGAAAA AACAATAAAT GGAATGGAAA ATAAATCTAT CTCTCCTGAT 
GAAGAAAGAC TTGCCTCAGA TTTAATTGAT CCTGTTGCTT TAGAAGCTCC GTTATCTAAA 
AATCTTGTGG CACAGATTAG CGCACTTGCT CTTCAACTAG ATTCAGAAGA TCTCTATAAT 
TATTCAGGAA GCCAGCTATT TGAAATGCAC GAGAAACTTG GTAGCATGGC AAACTCTATA 
ATTAAAAATC TGCAGTCACG TTGGAGGTCA CCAGCCCATG AAAATTCTTT TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RIF1 ( XM_010826489.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010824791.1 Bos taurus replication timing regulatory factor 1 (RIF1), transcript variant X2, mRNA.

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CloneID	OBa55065
Clone ID Related Accession (Same CDS sequence)	XM_010802073.3 , XM_010802073.2 , XM_010826489.2
Accession Version	XM_010826489.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7374bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	telomere-associated protein RIF1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644591.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010826489.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGCCG CGGATCAGAG CCCCCTGGCA CCGCTGTTGG AGACTTTGGA AGACCCTTCC 
GCCTCCCTTG GAGAGCAGAC CGACGCTTAC CTGACCCTGA CCAGTCGTAT GACTGGAGAA 
GATGGAAAAG AAATCATTAC AGAAATTGAG AAAAAACTTC CTCAGCTATT TAAAGTTTTA 
AAGACTCACA TTGCCAGTCA AAACTCAGAG CTCAGTAGTG CTGCTCTACA GGCCTTGGGG 
TTTTGCTTAT ATAATCCCAA AATTGCCTCT GGATTATCAG AGATAAATAT TCAAGAACTG 
CTTTCAAAAC TGAATGATAT TGTTAAAAGT TCAGATAAAA ATGTACGTAC TAGGGCACTT 
TGGGTGATAT CCAAGCAGAC GTTTCCCACT GAAGTCGTTG GCAAAGTAGT TTCTAGTATC 
ATTGACTCAC TAGAAATAGT ATTTAATAGA GGAGAGATGC TTTCTGCTGT TGTGGATTAT 
GAAGCATTAA ATGTCATTAT AAGACTAATT GAACAAGCCC CAGTTCAAAT GGGAGAAGAG 
TCAATTAGGT GGGCAAAATT GGTCATACCT TTACTGGTTC ATTCAGCCCA AAAAGTACAT 
TTGCGGGGAG CCACTGCTCT AGAGATGGGA ATGCCTTTGT TGCTTCAGAA ACAGCAAGAA 
ATAGCATCTA TCACAGAGCA GCTTATGACT ACCAAATTAC TTTCAGAACT CCAGAAACTG 
TTTATGAGTA AAAATGAGAC TTATGTGTTA AAATTGTGGC CTTTATTTGT CAAACTTCTT 
GGGAAGACCT TGCATCGAAG TGGGAGTTTC ATTAATTCTC TGTTACAGCT GGAAGAACTG 
GGATTTCGTA GTGGAGCACC CATGATTAAA AAAATAGCTT TTATTGCTTG GAAGAGTTTA 
ATAGATAATT TTGCTTTAAA TCCAGAAATA CTGTGTAGTG CAAAAAGACT GAAGTTGTTG 
ATGCAGCCTT TGAGTTCTAT CCATGTGAGA ACAGAAACTC TAGCGCTGAC AAAACTAGAA 
GTCTGGTGGT ATTTGTTGAT GAGACTAGGA CCTCATCTTC CTGCTAATTT TGAACAGGTT 
TGTGTGCCTC TGATTCAGAG CACAATAAGC ATTGATTCTA ATGCTTCACC TCAAGGGAGT 
TCATCTCGTG TAGCTACTAC TCCAGGTTTA AGCCCTATGA CCCCAGTACA CAAAGGTGCT 
TCTCCATATG GAACTCCTGT AACCCCTCGG ATGAACTTGA ATTCAAATTT TGGAATGGCC 
ACAATCCCCT CTATACAACT TTTGGGGCTT GAAATGTTGC TTCATTTTTT GTTGGGTCCA 
GAAGTCGTGA GTTTTGCTAA GCAAAACAAA CTTATACTGA GCCTAGAACC ACTTGAACAT 
CCATTAATCA GCAGCTCTTC TTTTTTCTCC AAACACTCAA ATACACTTAT CACTGCTGTT 
CATGATAGCT TTGTTGCAGT TGGAAAAGAT GCTTCTGATG TAGTTATTAA TGCTATTTGG 
AAGGAGTTAA TTAGCTTGGT GAAGTCAGTT ATTGAATCAG GTAACAAAAA AGAGAGACCA 
GGTTCTGAAG TTTTGACTCT CTTATTAAAA TCTTTGGAAA GCATAGTGAA TTCAGAAGTA 
TTTCCTGTAT TGAAAACACT GGTTCTCATG GAAATTACAA TTAAAGGACT TCCTCAGAAA 
GTATTAGGTT CTCCAGCATA TCAGGGAACC CCTGCTTTGT TCTTAATTCA GTTAACTTTC 
AATAATAATC TATTGGAATG TGGTGTGGAA GACGAAAGGT TTTTTCTCAA CCTGGAAACA 
CTTGTGGGCT GTGTTCTTTC GGGTCCAACT TCACCACTCG CTTTCAGTGA CTCTGTCTTA 
AATGTTGTCA ATCAAAATGC AAAGCAGTTG GATAACAAGG AGCATCTTTG GAGAATGTGG 
AGTATTATAG TCACCCCATT AACAGAATTG ATTAATCAGA CCAATGAAGT GAATCAAGGG 
GATGCCTTAG AACATAATTT CAGTGCTATC TATGGTGCAC TGATTTTGCC AATAAACCAT 
ATTTTTCCAG CACAAAAAAT TCCAGTGGTC ACCATGAAGA CCTTACTTAA AACTTGGTCA 
GAATTATATA GAGCATTTGC TCGCTGTGCA GCTTTGGTGG CAACTGCGGA AGAGAATTTA 
TGCTGTGAGG AATTTTGTTC CAAGATAATG TCCAGTTTGG AAGATGAAGG CCTTTCAAAT 
TTATTGTTCT TGGATAGGAT CACTCATATT ATTACTGTAA TGGTTGATTG CATCGACTTT 
TCACCATATA ATATTAAATA TCAACCCAAA ACTAAATCAC CACAGAGACC TACAGATTGG 
TCCAAAAAGA AGAAGGAACC TTTAGGGAAG TTGGCTTCTT TAATTAAACT TATTGTGAGA 
GTGATCTATT CTTTTCACAC ACTGAGCCTC AAGGAAGTAC ATTCTGATAC TCTCCTCACT 
ATTGGCAACT CAATCACCAG CATTCTTTCC AATTTACTTG GACATATTTC TCTGCCTTCT 
ATGATCCAGA AAATATTTGC AGCTTTAACA AGACCTCTGG CATTATTTTA TGAAAACTCA 
AAGCTTGATG AAGTTCCTAA AGTGTATAGT TGTATGAACA ACAAGTTAGA AAAGCTACTA 
GGAGATATAA TTGCTTGTCT ACATGTCAGC TACACTGGAA CTTACGACAG TGAACTTCTT 
GAACAGATCT CACCATTATT ATGCATAATA TTTTCACACA AGAACAAACA GATAAGAAAA 
CAAAGTGCTC AGTTCTGGAA TGCCACATTT GCTAAAGTGA CGATGTTGAT TTATCCTGAG 
GAGTTAAAGC CAATACTGAA ACAAGCCAAA CAAAAATCTC TGCTTTTATT GCCTGGTTTG 
GAAAATGTTG AAATTATGGA GGAATCCAGT GGACCATATT CAGATATGAC AGAAAATTCC 
CAATTAAATA TGAAGATAAG TGGCATGGAA AGAAAATCAA GTGGAAAAAG AGATTCTTTT 
TTGGCACAAA CAAAGGATAC AAAAGACAGT ATGAAACCAC CAGCTAAATT GAAACTAGAT 
TCTTCAACTC CAAAAGCAAA GGGTGAAATG CCTTTGGAAG AAGAAAAGTC TACTGACTTT 
GTGTTTATAC CTCCAGAAGT AAAAGAAACA AAGGAAAGAA TACTAACTGA ACACCAGAAA 
GAAGTACTCA AAACAAAGAG GTGTGATATT CCTGCCATGT ATAATAATCT GGATGTTTCA 
CAAGATACTT TATTTTCTCA GTATAGTCAG GAAGAATCTA TGGAAATTTC TACTTTAATT 
GAAAAATCAA AAGAAGACTC CAAGATGATA TTTAAGGAGG AACAAAAGGA GAATGACATT 
GTCGTTCCTC AACATGTCAT GGAAAACTGC GGTATGGATG AACATCTTGA AAAGGCTTCC 
CTTTTGAATA ATGAGTGTGG TTCTATTGAG GAAACCAGTC CAAAAATACT GAGCAGTAAT 
AATGATGCCA GAAAAAAAGC TTTAATTGCT TCAAATAAAC CAACAGAATG TGCATCTACT 
ACAGAAAATC CATCTGGTGT CAGCAGTAGC TCGGTTTCTA TTGCCACCAT TTCTGGAGCT 
CCCTTACAGC CTACAAGTCG AAGGCAATCC TTTATTACTT TGGAAAAGTT TGATGGTTCA 
GAAAATAGAC CTTTTAGTCC ATCACCCTTG AATAATATGT CATCAACTGT AATAGTGAAA 
AGTAACCAGG AAGGCATGGG TAAAACAGAT AATCCATCAA AAGCAAAGAA GAGAGAAGTG 
GCTCTTTCCA AATCTGACTC TGAAAAAATA ACACATGGGA CTAAGAGATC AAGCCGAAGG 
TCGAGTAAAT CTGAACCAAT AGGGAGTAAA AAGTCTAAAC CCTTAATGAG ATCTGAACAA 
GAGAAAAGTA CTCAAGAATC TGTTGATGGC ACAGTAGCCT TAGAAAATAA CCCACCTGGC 
TTGCTTAATC AGACAGAATG TGTGTTAGAT AATCAGGTTC ATCTCTCTGA ATCTACAGTG 
GAGTTTGAGG ATACAATGCT TGAATCAACA GTGGAGAATA CTGTATTAGA AAACAATACT 
GTAGAAGAGA GAACCTTAGA AGTTAACTTG GAATCCAAAG AAAACACACC CCCAGCCATA 
ATAACGGATC AGACAGTAAA TGAGGATAGT CATATTCAGG TAACTCCAAA TCAGAAAACC 
CTTAGGCGAT CTTTAAGGCG ACGTTCAGAA ACAGCAGAAC CAACCACTGA TAGCCAAGAT 
AAAGAAAATA ATCATCAAAA AAGGGAAAGA CGTAAAGAAG AAGAAAAGAC TCCGCAGAAG 
AGTCCGTTAC ATGTAAAAGA TGATGTTTTA CCTAAGCAAA AATTGATTTC TGAACAAAGT 
GTACAGGGAA ACTTAGTTGA CAAAGGAAAT AATTTACATG AGAAGGCTTT TGGGGAGACC 
AGTGCTAATG CTGAAATTGA TGAAAATAGA AGAAAGCCAG ATCTTGAGAA TACTAAATCT 
GAAGGAGATG GTGTGCCGGA CATTGCAGAT AAATCCTCTG AGAAACCAGT AAGGGGACGA 
ACACGATACC AAACAAGAAG AGCATCCCAG GGTTTACTTT CCAGCATTGA AAACTCAGAA 
TCTGATAGTT CTGAGGCAAA AGAAGAAGGT TCTAAAAAGA AAAGATCTGG AAAATGGAAG 
AACAAAAGCA GTGACAATGT TGACATGGAA GATCAAGAAG AGAAAATGGT AAAACAGGAA 
TGTATAAAAA TTGAAAATCA GGCACATGAT TATAAAGGGA ATCCTGAAGT AGACAAAGAT 
ATAAAATCTC AAATCTGTGA AAAAGGAGAT GAAAGTACTA CTGTAAGTCT TCAAGATTCT 
ACCGTATCTC CAGATTTATT ACAAGTTTAT GATGATGTAT TGAATACTTC TGAGGGAGAA 
AGTAAAAGTA ACAAAGATGC AGACCATTCC TTTTCAAATC CTTCTGTACC AGAATCAAAT 
CTAAGGACTA GAAATGCCAG TAAGAGGTTA CATAAGCGAG ATTCTATAGA AAATAGCATG 
GGGGAATCCT CAAAAATAGG GACATCAGAC ATTTCTTTGC TTTCTGAAAA AACTCTTCAG 
ACAGTAGAAT GCCAGCACAA GAGAAGTAGG AGGGTGAGGC GATCTAAAGG TTGTGATTGT 
TGTGGAGAAA AATCACAGTC TCAGGAAAAG TCATTTACTG GGTTAAAGGA TACAGAAAAT 
TACGATGTAA AAGTCAGTGA AACAAAAAAG ACAGATGGGG AAACACCTGT ATCTATTTCA 
GAAACTTCGA AAGCTGATCT GTCCTCGGAG GTTAAACTTT TAGATGAGCA TCATAGTGTG 
AATTTTCATT TGGATCTCAA GGAAGAGAAT GATACTACTA ATGATTCATT AATTATATCT 
GAAACTGAGT TAAAAGAAAA TACTACTCAA AGCTTTCTTC CTTCTGAAAT GGTAAAATTT 
AAAGAAACTT CTCGTAGGGA TTCTGAGGAA GCAATATCTC TAGAAACCCA GGAGCCATCT 
AATAAGAAAT GTAAAACTGT TGACCCACAT TTAGATGTTT CCAAAGATAT TTCACTGAAA 
TGTTTTTCTT TGGAGACTAA AGAAGAAAAG TCAGAGGCCA CTTCAAAAGA GAAATTGAGT 
ACAGAGAACA TTGTTATTGT TGAATCAAAT GCAGAATCAA ATCCAGAAGT AGATGCTATG 
GAATTGAATG TAGAAAATGA TCAGTCTGAA GCTGGCTTCT CTGAACAAAA GAATGTCAAA 
ACTGGTATTC CAGGGAAAGA AGTAACAGAG GAAAACAGTG AAAGAATGGA TACACCTGAA 
AAACTGAAAG CCAATGAAGC AGTAACTGAG GAATTTAATT CAGCTACTGA TTATTCGGAT 
ACTCCGACAT CTGTAAAAGT GAATGCTCAG GTAGAGATCT CTGAACAAAT AGCGGTTGGG 
GAGCTAGGTG GAGGAAGTGA TGAATCTGAT CCAGGCTCAT CTGAAGAAAT GAACGCTGAA 
ATGAAAAATT GTGAAAAAAC AGCGGTTTGT GAGGTGACTA CTGAATCTGA CCACGTCACT 
GAAACCGAAG ACGAAGATGT AACTACCACG GCTTCTCTGC CTATAGAAGT TGAAACAAAG 
GAATTAGATG TAGAAGTCAA TAACTTTGTT CCCGACACAC TTGAGATGAT CACTGAAGAA 
GGAAAGGTTG ACCCTAACAA AACTGAAACG AATACTGAAC CTAATAAATT GGAGGAAACA 
CAGTTAGATG ACAATCAGAT GGTAGTGGAA AGTAATGAAA CTTTACCGGA AGTTCACCAT 
ACTTCAGAGA AAGTAGAGGA GATAGCACAA CCTCTGACAT CTGAAACAGA TATTTCTGGA 
CTAATATCAG AAGACAGTAA TACATCTCCT CAAAAATCGA AGGATATTGA TCCTTTACTC 
ATGTCAGCAA ACGAGAGTCC TAGTGGCATG CACACACGTT GTGTGTGGTC TCCTTTGGCT 
TCTCCATCCA CCAGCATTTT AAAGAGAGGA CTAAAAAGAC CCCAAGAAGA TGAAATCTCG 
TCACCTGTCC ACAAGGTTCG CCGTGTCTCC TTTGCAGATC CTATATACCA AGCAGGATTG 
GCAGATGACA TTGATAGGCG ATGCTCTATT GTTAGGTGCC ATTCTTCAAA TAGTTCTCTC 
ATGGTAAAAA GTGTTAAAAC TTCACCTACT GTGCAATCTA AGCATAATAC CACTTCAGCC 
AAAGGATTTC TGTCCCCAGG ATCACGTAGC TCTAAATTTA AGAGCTCAAA GAAGTGTTTA 
ATTGCAGAAA TGGCTAAGGA ATCTGTGCCA TGCCCAACAG AAAGTGTTTA CCCTGCTTTG 
GTGAACTGTG TAGCACCAGT TGAAATCATT TTACCTCAGA TTACATCCAA CATGTGGGCA 
AGAGGACTGG GACAGCTCAT TAGAGCCAAG AATATAAAAA CAATTGGCGA TTTGAGTACC 
CTTACAGCAT CTGAAATAAA AACTCTTCCT ATCCGTTCTC CAAAAGTGTC TAACGTTAAA 
AAGGCTCTCA GAATATATCA TGAGCAGCAG GTGAAGTCTC GTGGACTAGA AGAGATTCCA 
GTTTTTGATA TTTCTGAAAA AACAATAAAT GGAATGGAAA ATAAATCTAT CTCTCCTGAT 
GAAGAAAGAC TTGCCTCAGA TTTAATTGAT CCTGTTGCTT TAGAAGCTCC GTTATCTAAA 
AATCTTGTGG CACAGATTAG CGCACTTGCT CTTCAACTAG ATTCAGAAGA TCTCTATAAT 
TATTCAGGAA GCCAGCTATT TGAAATGCAC GAGAAACTTG GTAGCATGGC AAACTCTATA 
ATTAAAAATC TGCAGTCACG TTGGAGGTCA CCAGCCCATG AAAATTCTTT TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010824791.1
CDS	146..7519
Translation	MTAADQSPLAPLLETLEDPSASLGEQTDAYLTLTSRMTGEDGKEIITEIEKKLPQLFKVLKTHIASQNSELSSAALQALGFCLYNPKIASGLSEINIQELLSKLNDIVKSSDKNVRTRALWVISKQTFPTEVVGKVVSSIIDSLEIVFNRGEMLSAVVDYEALNVIIRLIEQAPVQMGEESIRWAKLVIPLLVHSAQKVHLRGATALEMGMPLLLQKQQEIASITEQLMTTKLLSELQKLFMSKNETYVLKLWPLFVKLLGKTLHRSGSFINSLLQLEELGFRSGAPMIKKIAFIAWKSLIDNFALNPEILCSAKRLKLLMQPLSSIHVRTETLALTKLEVWWYLLMRLGPHLPANFEQVCVPLIQSTISIDSNASPQGSSSRVATTPGLSPMTPVHKGASPYGTPVTPRMNLNSNFGMATIPSIQLLGLEMLLHFLLGPEVVSFAKQNKLILSLEPLEHPLISSSSFFSKHSNTLITAVHDSFVAVGKDASDVVINAIWKELISLVKSVIESGNKKERPGSEVLTLLLKSLESIVNSEVFPVLKTLVLMEITIKGLPQKVLGSPAYQGTPALFLIQLTFNNNLLECGVEDERFFLNLETLVGCVLSGPTSPLAFSDSVLNVVNQNAKQLDNKEHLWRMWSIIVTPLTELINQTNEVNQGDALEHNFSAIYGALILPINHIFPAQKIPVVTMKTLLKTWSELYRAFARCAALVATAEENLCCEEFCSKIMSSLEDEGLSNLLFLDRITHIITVMVDCIDFSPYNIKYQPKTKSPQRPTDWSKKKKEPLGKLASLIKLIVRVIYSFHTLSLKEVHSDTLLTIGNSITSILSNLLGHISLPSMIQKIFAALTRPLALFYENSKLDEVPKVYSCMNNKLEKLLGDIIACLHVSYTGTYDSELLEQISPLLCIIFSHKNKQIRKQSAQFWNATFAKVTMLIYPEELKPILKQAKQKSLLLLPGLENVEIMEESSGPYSDMTENSQLNMKISGMERKSSGKRDSFLAQTKDTKDSMKPPAKLKLDSSTPKAKGEMPLEEEKSTDFVFIPPEVKETKERILTEHQKEVLKTKRCDIPAMYNNLDVSQDTLFSQYSQEESMEISTLIEKSKEDSKMIFKEEQKENDIVVPQHVMENCGMDEHLEKASLLNNECGSIEETSPKILSSNNDARKKALIASNKPTECASTTENPSGVSSSSVSIATISGAPLQPTSRRQSFITLEKFDGSENRPFSPSPLNNMSSTVIVKSNQEGMGKTDNPSKAKKREVALSKSDSEKITHGTKRSSRRSSKSEPIGSKKSKPLMRSEQEKSTQESVDGTVALENNPPGLLNQTECVLDNQVHLSESTVEFEDTMLESTVENTVLENNTVEERTLEVNLESKENTPPAIITDQTVNEDSHIQVTPNQKTLRRSLRRRSETAEPTTDSQDKENNHQKRERRKEEEKTPQKSPLHVKDDVLPKQKLISEQSVQGNLVDKGNNLHEKAFGETSANAEIDENRRKPDLENTKSEGDGVPDIADKSSEKPVRGRTRYQTRRASQGLLSSIENSESDSSEAKEEGSKKKRSGKWKNKSSDNVDMEDQEEKMVKQECIKIENQAHDYKGNPEVDKDIKSQICEKGDESTTVSLQDSTVSPDLLQVYDDVLNTSEGESKSNKDADHSFSNPSVPESNLRTRNASKRLHKRDSIENSMGESSKIGTSDISLLSEKTLQTVECQHKRSRRVRRSKGCDCCGEKSQSQEKSFTGLKDTENYDVKVSETKKTDGETPVSISETSKADLSSEVKLLDEHHSVNFHLDLKEENDTTNDSLIISETELKENTTQSFLPSEMVKFKETSRRDSEEAISLETQEPSNKKCKTVDPHLDVSKDISLKCFSLETKEEKSEATSKEKLSTENIVIVESNAESNPEVDAMELNVENDQSEAGFSEQKNVKTGIPGKEVTEENSERMDTPEKLKANEAVTEEFNSATDYSDTPTSVKVNAQVEISEQIAVGELGGGSDESDPGSSEEMNAEMKNCEKTAVCEVTTESDHVTETEDEDVTTTASLPIEVETKELDVEVNNFVPDTLEMITEEGKVDPNKTETNTEPNKLEETQLDDNQMVVESNETLPEVHHTSEKVEEIAQPLTSETDISGLISEDSNTSPQKSKDIDPLLMSANESPSGMHTRCVWSPLASPSTSILKRGLKRPQEDEISSPVHKVRRVSFADPIYQAGLADDIDRRCSIVRCHSSNSSLMVKSVKTSPTVQSKHNTTSAKGFLSPGSRSSKFKSSKKCLIAEMAKESVPCPTESVYPALVNCVAPVEIILPQITSNMWARGLGQLIRAKNIKTIGDLSTLTASEIKTLPIRSPKVSNVKKALRIYHEQQVKSRGLEEIPVFDISEKTINGMENKSISPDEERLASDLIDPVALEAPLSKNLVAQISALALQLDSEDLYNYSGSQLFEMHEKLGSMANSIIKNLQSRWRSPAHENSF

Target ORF information:

RefSeq Version	XM_010826489.2
Organism	Bos taurus(cattle)
Definition	Bos taurus replication timing regulatory factor 1 (RIF1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010826489.2

ORF Insert Sequence:

ATGACGGCCG CGGATCAGAG CCCCCTGGCA CCGCTGTTGG AGACTTTGGA AGACCCTTCC 
GCCTCCCTTG GAGAGCAGAC CGACGCTTAC CTGACCCTGA CCAGTCGTAT GACTGGAGAA 
GATGGAAAAG AAATCATTAC AGAAATTGAG AAAAAACTTC CTCAGCTATT TAAAGTTTTA 
AAGACTCACA TTGCCAGTCA AAACTCAGAG CTCAGTAGTG CTGCTCTACA GGCCTTGGGG 
TTTTGCTTAT ATAATCCCAA AATTGCCTCT GGATTATCAG AGATAAATAT TCAAGAACTG 
CTTTCAAAAC TGAATGATAT TGTTAAAAGT TCAGATAAAA ATGTACGTAC TAGGGCACTT 
TGGGTGATAT CCAAGCAGAC GTTTCCCACT GAAGTCGTTG GCAAAGTAGT TTCTAGTATC 
ATTGACTCAC TAGAAATAGT ATTTAATAGA GGAGAGATGC TTTCTGCTGT TGTGGATTAT 
GAAGCATTAA ATGTCATTAT AAGACTAATT GAACAAGCCC CAGTTCAAAT GGGAGAAGAG 
TCAATTAGGT GGGCAAAATT GGTCATACCT TTACTGGTTC ATTCAGCCCA AAAAGTACAT 
TTGCGGGGAG CCACTGCTCT AGAGATGGGA ATGCCTTTGT TGCTTCAGAA ACAGCAAGAA 
ATAGCATCTA TCACAGAGCA GCTTATGACT ACCAAATTAC TTTCAGAACT CCAGAAACTG 
TTTATGAGTA AAAATGAGAC TTATGTGTTA AAATTGTGGC CTTTATTTGT CAAACTTCTT 
GGGAAGACCT TGCATCGAAG TGGGAGTTTC ATTAATTCTC TGTTACAGCT GGAAGAACTG 
GGATTTCGTA GTGGAGCACC CATGATTAAA AAAATAGCTT TTATTGCTTG GAAGAGTTTA 
ATAGATAATT TTGCTTTAAA TCCAGAAATA CTGTGTAGTG CAAAAAGACT GAAGTTGTTG 
ATGCAGCCTT TGAGTTCTAT CCATGTGAGA ACAGAAACTC TAGCGCTGAC AAAACTAGAA 
GTCTGGTGGT ATTTGTTGAT GAGACTAGGA CCTCATCTTC CTGCTAATTT TGAACAGGTT 
TGTGTGCCTC TGATTCAGAG CACAATAAGC ATTGATTCTA ATGCTTCACC TCAAGGGAGT 
TCATCTCGTG TAGCTACTAC TCCAGGTTTA AGCCCTATGA CCCCAGTACA CAAAGGTGCT 
TCTCCATATG GAACTCCTGT AACCCCTCGG ATGAACTTGA ATTCAAATTT TGGAATGGCC 
ACAATCCCCT CTATACAACT TTTGGGGCTT GAAATGTTGC TTCATTTTTT GTTGGGTCCA 
GAAGTCGTGA GTTTTGCTAA GCAAAACAAA CTTATACTGA GCCTAGAACC ACTTGAACAT 
CCATTAATCA GCAGCTCTTC TTTTTTCTCC AAACACTCAA ATACACTTAT CACTGCTGTT 
CATGATAGCT TTGTTGCAGT TGGAAAAGAT GCTTCTGATG TAGTTATTAA TGCTATTTGG 
AAGGAGTTAA TTAGCTTGGT GAAGTCAGTT ATTGAATCAG GTAACAAAAA AGAGAGACCA 
GGTTCTGAAG TTTTGACTCT CTTATTAAAA TCTTTGGAAA GCATAGTGAA TTCAGAAGTA 
TTTCCTGTAT TGAAAACACT GGTTCTCATG GAAATTACAA TTAAAGGACT TCCTCAGAAA 
GTATTAGGTT CTCCAGCATA TCAGGGAACC CCTGCTTTGT TCTTAATTCA GTTAACTTTC 
AATAATAATC TATTGGAATG TGGTGTGGAA GACGAAAGGT TTTTTCTCAA CCTGGAAACA 
CTTGTGGGCT GTGTTCTTTC GGGTCCAACT TCACCACTCG CTTTCAGTGA CTCTGTCTTA 
AATGTTGTCA ATCAAAATGC AAAGCAGTTG GATAACAAGG AGCATCTTTG GAGAATGTGG 
AGTATTATAG TCACCCCATT AACAGAATTG ATTAATCAGA CCAATGAAGT GAATCAAGGG 
GATGCCTTAG AACATAATTT CAGTGCTATC TATGGTGCAC TGATTTTGCC AATAAACCAT 
ATTTTTCCAG CACAAAAAAT TCCAGTGGTC ACCATGAAGA CCTTACTTAA AACTTGGTCA 
GAATTATATA GAGCATTTGC TCGCTGTGCA GCTTTGGTGG CAACTGCGGA AGAGAATTTA 
TGCTGTGAGG AATTTTGTTC CAAGATAATG TCCAGTTTGG AAGATGAAGG CCTTTCAAAT 
TTATTGTTCT TGGATAGGAT CACTCATATT ATTACTGTAA TGGTTGATTG CATCGACTTT 
TCACCATATA ATATTAAATA TCAACCCAAA ACTAAATCAC CACAGAGACC TACAGATTGG 
TCCAAAAAGA AGAAGGAACC TTTAGGGAAG TTGGCTTCTT TAATTAAACT TATTGTGAGA 
GTGATCTATT CTTTTCACAC ACTGAGCCTC AAGGAAGTAC ATTCTGATAC TCTCCTCACT 
ATTGGCAACT CAATCACCAG CATTCTTTCC AATTTACTTG GACATATTTC TCTGCCTTCT 
ATGATCCAGA AAATATTTGC AGCTTTAACA AGACCTCTGG CATTATTTTA TGAAAACTCA 
AAGCTTGATG AAGTTCCTAA AGTGTATAGT TGTATGAACA ACAAGTTAGA AAAGCTACTA 
GGAGATATAA TTGCTTGTCT ACATGTCAGC TACACTGGAA CTTACGACAG TGAACTTCTT 
GAACAGATCT CACCATTATT ATGCATAATA TTTTCACACA AGAACAAACA GATAAGAAAA 
CAAAGTGCTC AGTTCTGGAA TGCCACATTT GCTAAAGTGA CGATGTTGAT TTATCCTGAG 
GAGTTAAAGC CAATACTGAA ACAAGCCAAA CAAAAATCTC TGCTTTTATT GCCTGGTTTG 
GAAAATGTTG AAATTATGGA GGAATCCAGT GGACCATATT CAGATATGAC AGAAAATTCC 
CAATTAAATA TGAAGATAAG TGGCATGGAA AGAAAATCAA GTGGAAAAAG AGATTCTTTT 
TTGGCACAAA CAAAGGATAC AAAAGACAGT ATGAAACCAC CAGCTAAATT GAAACTAGAT 
TCTTCAACTC CAAAAGCAAA GGGTGAAATG CCTTTGGAAG AAGAAAAGTC TACTGACTTT 
GTGTTTATAC CTCCAGAAGT AAAAGAAACA AAGGAAAGAA TACTAACTGA ACACCAGAAA 
GAAGTACTCA AAACAAAGAG GTGTGATATT CCTGCCATGT ATAATAATCT GGATGTTTCA 
CAAGATACTT TATTTTCTCA GTATAGTCAG GAAGAATCTA TGGAAATTTC TACTTTAATT 
GAAAAATCAA AAGAAGACTC CAAGATGATA TTTAAGGAGG AACAAAAGGA GAATGACATT 
GTCGTTCCTC AACATGTCAT GGAAAACTGC GGTATGGATG AACATCTTGA AAAGGCTTCC 
CTTTTGAATA ATGAGTGTGG TTCTATTGAG GAAACCAGTC CAAAAATACT GAGCAGTAAT 
AATGATGCCA GAAAAAAAGC TTTAATTGCT TCAAATAAAC CAACAGAATG TGCATCTACT 
ACAGAAAATC CATCTGGTGT CAGCAGTAGC TCGGTTTCTA TTGCCACCAT TTCTGGAGCT 
CCCTTACAGC CTACAAGTCG AAGGCAATCC TTTATTACTT TGGAAAAGTT TGATGGTTCA 
GAAAATAGAC CTTTTAGTCC ATCACCCTTG AATAATATGT CATCAACTGT AATAGTGAAA 
AGTAACCAGG AAGGCATGGG TAAAACAGAT AATCCATCAA AAGCAAAGAA GAGAGAAGTG 
GCTCTTTCCA AATCTGACTC TGAAAAAATA ACACATGGGA CTAAGAGATC AAGCCGAAGG 
TCGAGTAAAT CTGAACCAAT AGGGAGTAAA AAGTCTAAAC CCTTAATGAG ATCTGAACAA 
GAGAAAAGTA CTCAAGAATC TGTTGATGGC ACAGTAGCCT TAGAAAATAA CCCACCTGGC 
TTGCTTAATC AGACAGAATG TGTGTTAGAT AATCAGGTTC ATCTCTCTGA ATCTACAGTG 
GAGTTTGAGG ATACAATGCT TGAATCAACA GTGGAGAATA CTGTATTAGA AAACAATACT 
GTAGAAGAGA GAACCTTAGA AGTTAACTTG GAATCCAAAG AAAACACACC CCCAGCCATA 
ATAACGGATC AGACAGTAAA TGAGGATAGT CATATTCAGG TAACTCCAAA TCAGAAAACC 
CTTAGGCGAT CTTTAAGGCG ACGTTCAGAA ACAGCAGAAC CAACCACTGA TAGCCAAGAT 
AAAGAAAATA ATCATCAAAA AAGGGAAAGA CGTAAAGAAG AAGAAAAGAC TCCGCAGAAG 
AGTCCGTTAC ATGTAAAAGA TGATGTTTTA CCTAAGCAAA AATTGATTTC TGAACAAAGT 
GTACAGGGAA ACTTAGTTGA CAAAGGAAAT AATTTACATG AGAAGGCTTT TGGGGAGACC 
AGTGCTAATG CTGAAATTGA TGAAAATAGA AGAAAGCCAG ATCTTGAGAA TACTAAATCT 
GAAGGAGATG GTGTGCCGGA CATTGCAGAT AAATCCTCTG AGAAACCAGT AAGGGGACGA 
ACACGATACC AAACAAGAAG AGCATCCCAG GGTTTACTTT CCAGCATTGA AAACTCAGAA 
TCTGATAGTT CTGAGGCAAA AGAAGAAGGT TCTAAAAAGA AAAGATCTGG AAAATGGAAG 
AACAAAAGCA GTGACAATGT TGACATGGAA GATCAAGAAG AGAAAATGGT AAAACAGGAA 
TGTATAAAAA TTGAAAATCA GGCACATGAT TATAAAGGGA ATCCTGAAGT AGACAAAGAT 
ATAAAATCTC AAATCTGTGA AAAAGGAGAT GAAAGTACTA CTGTAAGTCT TCAAGATTCT 
ACCGTATCTC CAGATTTATT ACAAGTTTAT GATGATGTAT TGAATACTTC TGAGGGAGAA 
AGTAAAAGTA ACAAAGATGC AGACCATTCC TTTTCAAATC CTTCTGTACC AGAATCAAAT 
CTAAGGACTA GAAATGCCAG TAAGAGGTTA CATAAGCGAG ATTCTATAGA AAATAGCATG 
GGGGAATCCT CAAAAATAGG GACATCAGAC ATTTCTTTGC TTTCTGAAAA AACTCTTCAG 
ACAGTAGAAT GCCAGCACAA GAGAAGTAGG AGGGTGAGGC GATCTAAAGG TTGTGATTGT 
TGTGGAGAAA AATCACAGTC TCAGGAAAAG TCATTTACTG GGTTAAAGGA TACAGAAAAT 
TACGATGTAA AAGTCAGTGA AACAAAAAAG ACAGATGGGG AAACACCTGT ATCTATTTCA 
GAAACTTCGA AAGCTGATCT GTCCTCGGAG GTTAAACTTT TAGATGAGCA TCATAGTGTG 
AATTTTCATT TGGATCTCAA GGAAGAGAAT GATACTACTA ATGATTCATT AATTATATCT 
GAAACTGAGT TAAAAGAAAA TACTACTCAA AGCTTTCTTC CTTCTGAAAT GGTAAAATTT 
AAAGAAACTT CTCGTAGGGA TTCTGAGGAA GCAATATCTC TAGAAACCCA GGAGCCATCT 
AATAAGAAAT GTAAAACTGT TGACCCACAT TTAGATGTTT CCAAAGATAT TTCACTGAAA 
TGTTTTTCTT TGGAGACTAA AGAAGAAAAG TCAGAGGCCA CTTCAAAAGA GAAATTGAGT 
ACAGAGAACA TTGTTATTGT TGAATCAAAT GCAGAATCAA ATCCAGAAGT AGATGCTATG 
GAATTGAATG TAGAAAATGA TCAGTCTGAA GCTGGCTTCT CTGAACAAAA GAATGTCAAA 
ACTGGTATTC CAGGGAAAGA AGTAACAGAG GAAAACAGTG AAAGAATGGA TACACCTGAA 
AAACTGAAAG CCAATGAAGC AGTAACTGAG GAATTTAATT CAGCTACTGA TTATTCGGAT 
ACTCCGACAT CTGTAAAAGT GAATGCTCAG GTAGAGATCT CTGAACAAAT AGCGGTTGGG 
GAGCTAGGTG GAGGAAGTGA TGAATCTGAT CCAGGCTCAT CTGAAGAAAT GAACGCTGAA 
ATGAAAAATT GTGAAAAAAC AGCGGTTTGT GAGGTGACTA CTGAATCTGA CCACGTCACT 
GAAACCGAAG ACGAAGATGT AACTACCACG GCTTCTCTGC CTATAGAAGT TGAAACAAAG 
GAATTAGATG TAGAAGTCAA TAACTTTGTT CCCGACACAC TTGAGATGAT CACTGAAGAA 
GGAAAGGTTG ACCCTAACAA AACTGAAACG AATACTGAAC CTAATAAATT GGAGGAAACA 
CAGTTAGATG ACAATCAGAT GGTAGTGGAA AGTAATGAAA CTTTACCGGA AGTTCACCAT 
ACTTCAGAGA AAGTAGAGGA GATAGCACAA CCTCTGACAT CTGAAACAGA TATTTCTGGA 
CTAATATCAG AAGACAGTAA TACATCTCCT CAAAAATCGA AGGATATTGA TCCTTTACTC 
ATGTCAGCAA ACGAGAGTCC TAGTGGCATG CACACACGTT GTGTGTGGTC TCCTTTGGCT 
TCTCCATCCA CCAGCATTTT AAAGAGAGGA CTAAAAAGAC CCCAAGAAGA TGAAATCTCG 
TCACCTGTCC ACAAGGTTCG CCGTGTCTCC TTTGCAGATC CTATATACCA AGCAGGATTG 
GCAGATGACA TTGATAGGCG ATGCTCTATT GTTAGGTGCC ATTCTTCAAA TAGTTCTCTC 
ATGGTAAAAA GTGTTAAAAC TTCACCTACT GTGCAATCTA AGCATAATAC CACTTCAGCC 
AAAGGATTTC TGTCCCCAGG ATCACGTAGC TCTAAATTTA AGAGCTCAAA GAAGTGTTTA 
ATTGCAGAAA TGGCTAAGGA ATCTGTGCCA TGCCCAACAG AAAGTGTTTA CCCTGCTTTG 
GTGAACTGTG TAGCACCAGT TGAAATCATT TTACCTCAGA TTACATCCAA CATGTGGGCA 
AGAGGACTGG GACAGCTCAT TAGAGCCAAG AATATAAAAA CAATTGGCGA TTTGAGTACC 
CTTACAGCAT CTGAAATAAA AACTCTTCCT ATCCGTTCTC CAAAAGTGTC TAACGTTAAA 
AAGGCTCTCA GAATATATCA TGAGCAGCAG GTGAAGTCTC GTGGACTAGA AGAGATTCCA 
GTTTTTGATA TTTCTGAAAA AACAATAAAT GGAATGGAAA ATAAATCTAT CTCTCCTGAT 
GAAGAAAGAC TTGCCTCAGA TTTAATTGAT CCTGTTGCTT TAGAAGCTCC GTTATCTAAA 
AATCTTGTGG CACAGATTAG CGCACTTGCT CTTCAACTAG ATTCAGAAGA TCTCTATAAT 
TATTCAGGAA GCCAGCTATT TGAAATGCAC GAGAAACTTG GTAGCATGGC AAACTCTATA 
ATTAAAAATC TGCAGTCACG TTGGAGGTCA CCAGCCCATG AAAATTCTTT TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RIF1 ( XM_010802074.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010800376.1 Bos taurus replication timing regulatory factor 1 (RIF1), transcript variant X3, mRNA.

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CloneID	OBa55066
Clone ID Related Accession (Same CDS sequence)	XM_010802074.3 , XM_010802074.2 , XM_010826490.2
Accession Version	XM_010802074.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7320bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	telomere-associated protein RIF1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010802074.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGCCG CGGATCAGAG CCCCCTGGCA CCGCTGTTGG AGACTTTGGA AGACCCTTCC 
GCCTCCCTTG GAGAGCAGAC CGACGCTTAC CTGACCCTGA CCAGTCGTAT GACTGGAGAA 
GATGGAAAAG AAATCATTAC AGAAATTGAG AAAAAACTTC CTCAGCTATT TAAAGTTTTA 
AAGACTCACA TTGCCAGTCA AAACTCAGAG CTCAGTAGTG CTGCTCTACA GGCCTTGGGG 
TTTTGCTTAT ATAATCCCAA AATTGCCTCT GGATTATCAG AGATAAATAT TCAAGAACTG 
CTTTCAAAAC TGAATGATAT TGTTAAAAGT TCAGATAAAA ATGTACGTAC TAGGGCACTT 
TGGGTGATAT CCAAGCAGAC GTTTCCCACT GAAGTCGTTG GCAAAGTAGT TTCTAGTATC 
ATTGACTCAC TAGAAATAGT ATTTAATAGA GGAGAGATGC TTTCTGCTGT TGTGGATTAT 
GAAGCATTAA ATGTCATTAT AAGACTAATT GAACAAGCCC CAGTTCAAAT GGGAGAAGAG 
TCAATTAGGT GGGCAAAATT GGTCATACCT TTACTGGTTC ATTCAGCCCA AAAAGTACAT 
TTGCGGGGAG CCACTGCTCT AGAGATGGGA ATGCCTTTGT TGCTTCAGAA ACAGCAAGAA 
ATAGCATCTA TCACAGAGCA GCTTATGACT ACCAAATTAC TTTCAGAACT CCAGAAACTG 
TTTATGAGTA AAAATGAGAC TTATGTGTTA AAATTGTGGC CTTTATTTGT CAAACTTCTT 
GGGAAGACCT TGCATCGAAG TGGGAGTTTC ATTAATTCTC TGTTACAGCT GGAAGAACTG 
GGATTTCGTA GTGGAGCACC CATGATTAAA AAAATAGCTT TTATTGCTTG GAAGAGTTTA 
ATAGATAATT TTGCTTTAAA TCCAGAAATA CTGTGTAGTG CAAAAAGACT GAAGTTGTTG 
ATGCAGCCTT TGAGTTCTAT CCATGTGAGA ACAGAAACTC TAGCGCTGAC AAAACTAGAA 
GTCTGGTGGT ATTTGTTGAT GAGACTAGGA CCTCATCTTC CTGCTAATTT TGAACAGGTT 
TGTGTGCCTC TGATTCAGAG CACAATAAGC ATTGATTCTA ATGCTTCACC TCAAGGGAGT 
TCATCTCGTG TAGCTACTAC TCCAGGTTTA AGCCCTATGA CCCCAGTACA CAAAGGTGCT 
TCTCCATATG GAACTCCTGT AACCCCTCGG ATGAACTTGA ATTCAAATTT TGGAATGGCC 
ACAATCCCCT CTATACAACT TTTGGGGCTT GAAATGTTGC TTCATTTTTT GTTGGGTCCA 
GAAGTCGTGA GTTTTGCTAA GCAAAACAAA CTTATACTGA GCCTAGAACC ACTTGAACAT 
CCATTAATCA GCAGCTCTTC TTTTTTCTCC AAACACTCAA ATACACTTAT CACTGCTGTT 
CATGATAGCT TTGTTGCAGT TGGAAAAGAT GCTTCTGATG TAGTTATTAA TGCTATTTGG 
AAGGAGTTAA TTAGCTTGGT GAAGTCAGTT ATTGAATCAG GTAACAAAAA AGAGAGACCA 
GGTTCTGAAG TTTTGACTCT CTTATTAAAA TCTTTGGAAA GCATAGTGAA TTCAGAAGTA 
TTTCCTGTAT TGAAAACACT GGTTCTCATG GAAATTACAA TTAAAGGACT TCCTCAGAAA 
GTATTAGGTT CTCCAGCATA TCAGGTTGCT AATATGGATA TTCTTAATGG AACCCCTGCT 
TTGTTCTTAA TTCAGTTAAC TTTCAATAAT AATCTATTGG AATGTGGTGT GGAAGACGAA 
AGGTTTTTTC TCAACCTGGA AACACTTGTG GGCTGTGTTC TTTCGGGTCC AACTTCACCA 
CTCGCTTTCA GTGACTCTGT CTTAAATGTT GTCAATCAAA ATGCAAAGCA GTTGGATAAC 
AAGGAGCATC TTTGGAGAAT GTGGAGTATT ATAGTCACCC CATTAACAGA ATTGATTAAT 
CAGACCAATG AAGTGAATCA AGGGGATGCC TTAGAACATA ATTTCAGTGC TATCTATGGT 
GCACTGATTT TGCCAATAAA CCATATTTTT CCAGCACAAA AAATTCCAGT GGTCACCATG 
AAGACCTTAC TTAAAACTTG GTCAGAATTA TATAGAGCAT TTGCTCGCTG TGCAGCTTTG 
GTGGCAACTG CGGAAGAGAA TTTATGCTGT GAGGAATTTT GTTCCAAGAT AATGTCCAGT 
TTGGAAGATG AAGGCCTTTC AAATTTATTG TTCTTGGATA GGATCACTCA TATTATTACT 
GTAATGGTTG ATTGCATCGA CTTTTCACCA TATAATATTA AATATCAACC CAAAACTAAA 
TCACCACAGA GACCTACAGA TTGGTCCAAA AAGAAGAAGG AACCTTTAGG GAAGTTGGCT 
TCTTTAATTA AACTTATTGT GAGAGTGATC TATTCTTTTC ACACACTGAG CCTCAAGGAA 
GTACATTCTG ATACTCTCCT CACTATTGGC AACTCAATCA CCAGCATTCT TTCCAATTTA 
CTTGGACATA TTTCTCTGCC TTCTATGATC CAGAAAATAT TTGCAGCTTT AACAAGACCT 
CTGGCATTAT TTTATGAAAA CTCAAAGCTT GATGAAGTTC CTAAAGTGTA TAGTTGTATG 
AACAACAAGT TAGAAAAGCT ACTAGGAGAT ATAATTGCTT GTCTACATGT CAGCTACACT 
GGAACTTACG ACAGTGAACT TCTTGAACAG ATCTCACCAT TATTATGCAT AATATTTTCA 
CACAAGAACA AACAGATAAG AAAACAAAGT GCTCAGTTCT GGAATGCCAC ATTTGCTAAA 
GTGACGATGT TGATTTATCC TGAGGAGTTA AAGCCAATAC TGAAACAAGC CAAACAAAAA 
TCTCTGCTTT TATTGCCTGG TTTGGAAAAT GTTGAAATTA TGGAGGAATC CAGTGGACCA 
TATTCAGATA TGACAGAAAA TTCCCAATTA AATATGAAGA TAAGTGGCAT GGAAAGAAAA 
TCAAGTGGAA AAAGAGATTC TTTTTTGGCA CAAACAAAGG ATACAAAAGA CAGTATGAAA 
CCACCAGCTA AATTGAAACT AGATTCTTCA ACTCCAAAAG CAAAGGGTGA AATGCCTTTG 
GAAGAAGAAA AGTCTACTGA CTTTGTGTTT ATACCTCCAG AAGTAAAAGA AACAAAGGAA 
AGAATACTAA CTGAACACCA GAAAGAAGTA CTCAAAACAA AGAGGTGTGA TATTCCTGCC 
ATGTATAATA ATCTGGATGT TTCACAAGAT ACTTTATTTT CTCAGTATAG TCAGGAAGAA 
TCTATGGAAA TTTCTACTTT AATTGAAAAA TCAAAAGAAG ACTCCAAGAT GATATTTAAG 
GAGGAACAAA AGGAGAATGA CATTGTCGTT CCTCAACATG TCATGGAAAA CTGCGGTATG 
GATGAACATC TTGAAAAGGC TTCCCTTTTG AATAATGAGT GTGGTTCTAT TGAGGAAACC 
AGTCCAAAAA TACTGAGCAG TAATAATGAT GCCAGAAAAA AAGCTTTAAT TGCTTCAAAT 
AAACCAACAG AATGTGCATC TACTACAGAA AATCCATCTG GTGTCAGCAG TAGCTCGGTT 
TCTATTGCCA CCATTTCTGG AGCTCCCTTA CAGCCTACAA GTCGAAGGCA ATCCTTTATT 
ACTTTGGAAA AGTTTGATGG TTCAGAAAAT AGACCTTTTA GTCCATCACC CTTGAATAAT 
ATGTCATCAA CTGTAATAGT GAAAAGTAAC CAGGAAGGCA TGGGTAAAAC AGATAATCCA 
TCAAAAGCAA AGAAGAGAGA AGTGGCTCTT TCCAAATCTG ACTCTGAAAA AATAACACAT 
GGGACTAAGA GATCAAGCCG AAGGTCGAGT AAATCTGAAC CAATAGGGAG TAAAAAGTCT 
AAACCCTTAA TGAGATCTGA ACAAGAGAAA AGTACTCAAG AATCTGTTGA TGGCACAGTA 
GCCTTAGAAA ATAACCCACC TGGCTTGCTT AATCAGACAG AATGTGTGTT AGATAATCAG 
GTTCATCTCT CTGAATCTAC AGTGGAGTTT GAGGATACAA TGCTTGAATC AACAGTGGAG 
AATACTGTAT TAGAAAACAA TACTGTAGAA GAGAGAACCT TAGAAGTTAA CTTGGAATCC 
AAAGAAAACA CACCCCCAGC CATAATAACG GATCAGACAG TAAATGAGGA TAGTCATATT 
CAGGTAACTC CAAATCAGAA AACCCTTAGG CGATCTTTAA GGCGACGTTC AGAAACAGCA 
GAACCAACCA CTGATAGCCA AGATAAAGAA AATAATCATC AAAAAAGGGA AAGACGTAAA 
GAAGAAGAAA AGACTCCGCA GAAGAGTCCG TTACATGTAA AAGATGATGT TTTACCTAAG 
CAAAAATTGA TTTCTGAACA AAGTGTACAG GGAAACTTAG TTGACAAAGG AAATAATTTA 
CATGAGAAGG CTTTTGGGGA GACCAGTGCT AATGCTGAAA TTGATGAAAA TAGAAGAAAG 
CCAGATCTTG AGAATACTAA ATCTGAAGGA GATGGTGTGC CGGACATTGC AGATAAATCC 
TCTGAGAAAC CAGTAAGGGG ACGAACACGA TACCAAACAA GAAGAGCATC CCAGGGTTTA 
CTTTCCAGCA TTGAAAACTC AGAATCTGAT AGTTCTGAGG CAAAAGAAGA AGGTTCTAAA 
AAGAAAAGAT CTGGAAAATG GAAGAACAAA AGCAGTGACA ATGTTGACAT GGAAGATCAA 
GAAGAGAAAA TGGTAAAACA GGAATGTATA AAAATTGAAA ATCAGGCACA TGATTATAAA 
GGGAATCCTG AAGTAGACAA AGATATAAAA TCTCAAATCT GTGAAAAAGG AGATGAAAGT 
ACTACTGTAA GTCTTCAAGA TTCTACCGTA TCTCCAGATT TATTACAAGT TTATGATGAT 
GTATTGAATA CTTCTGAGGG AGAAAGTAAA AGTAACAAAG ATGCAGACCA TTCCTTTTCA 
AATCCTTCTG TACCAGAATC AAATCTAAGG ACTAGAAATG CCAGTAAGAG GTTACATAAG 
CGAGATTCTA TAGAAAATAG CATGGGGGAA TCCTCAAAAA TAGGGACATC AGACATTTCT 
TTGCTTTCTG AAAAAACTCT TCAGACAGTA GAATGCCAGC ACAAGAGAAG TAGGAGGGTG 
AGGCGATCTA AAGGTTGTGA TTGTTGTGGA GAAAAATCAC AGTCTCAGGA AAAGTCATTT 
ACTGGGTTAA AGGATACAGA AAATTACGAT GTAAAAGTCA GTGAAACAAA AAAGACAGAT 
GGGGAAACAC CTGTATCTAT TTCAGAAACT TCGAAAGCTG ATCTGTCCTC GGAGGTTAAA 
CTTTTAGATG AGCATCATAG TGTGAATTTT CATTTGGATC TCAAGGAAGA GAATGATACT 
ACTAATGATT CATTAATTAT ATCTGAAACT GAGTTAAAAG AAAATACTAC TCAAAGCTTT 
CTTCCTTCTG AAATGGTAAA ATTTAAAGAA ACTTCTCGTA GGGATTCTGA GGAAGCAATA 
TCTCTAGAAA CCCAGGAGCC ATCTAATAAG AAATGTAAAA CTGTTGACCC ACATTTAGAT 
GTTTCCAAAG ATATTTCACT GAAATGTTTT TCTTTGGAGA CTAAAGAAGA AAAGTCAGAG 
GCCACTTCAA AAGAGAAATT GAGTACAGAG AACATTGTTA TTGTTGAATC AAATGCAGAA 
TCAAATCCAG AAGTAGATGC TATGGAATTG AATGTAGAAA ATGATCAGTC TGAAGCTGGC 
TTCTCTGAAC AAAAGAATGT CAAAACTGGT ATTCCAGGGA AAGAAGTAAC AGAGGAAAAC 
AGTGAAAGAA TGGATACACC TGAAAAACTG AAAGCCAATG AAGCAGTAAC TGAGGAATTT 
AATTCAGCTA CTGATTATTC GGATACTCCG ACATCTGTAA AAGTGAATGC TCAGGTAGAG 
ATCTCTGAAC AAATAGCGGT TGGGGAGCTA GGTGGAGGAA GTGATGAATC TGATCCAGGC 
TCATCTGAAG AAATGAACGC TGAAATGAAA AATTGTGAAA AAACAGCGGT TTGTGAGGTG 
ACTACTGAAT CTGACCACGT CACTGAAACC GAAGACGAAG ATGTAACTAC CACGGCTTCT 
CTGCCTATAG AAGTTGAAAC AAAGGAATTA GATGTAGAAG TCAATAACTT TGTTCCCGAC 
ACACTTGAGA TGATCACTGA AGAAGGAAAG GTTGACCCTA ACAAAACTGA AACGAATACT 
GAACCTAATA AATTGGAGGA AACACAGTTA GATGACAATC AGATGGTAGT GGAAAGTAAT 
GAAACTTTAC CGGAAGTTCA CCATACTTCA GAGAAAGTAG AGGAGATAGC ACAACCTCTG 
ACATCTGAAA CAGATATTTC TGGACTAATA TCAGAAGACA GTAATACATC TCCTCAAAAA 
TCGAAGGATA TTGATCCTTT ACTCATGTCA GCAAACGAGA GTCCTAGTGG CATGCACACA 
CGTTGTGTGT GGTCTCCTTT GGCTTCTCCA TCCACCAGCA TTTTAAAGAG AGGACTAAAA 
AGACCCCAAG AAGATGAAAT CTCGTCACCT GTCCACAAGG TTCGCCGTGT CTCCTTTGCA 
GATCCTATAT ACCAAGCAGG ATTGGCAGAT GACATTGATA GGCGATGCTC TATTGTTAGG 
TGCCATTCTT CAAATAGTTC TCTCATGGTA AAAAGTGTTA AAACTTCACC TACTGTGCAA 
TCTAAGATTG CAGAAATGGC TAAGGAATCT GTGCCATGCC CAACAGAAAG TGTTTACCCT 
GCTTTGGTGA ACTGTGTAGC ACCAGTTGAA ATCATTTTAC CTCAGATTAC ATCCAACATG 
TGGGCAAGAG GACTGGGACA GCTCATTAGA GCCAAGAATA TAAAAACAAT TGGCGATTTG 
AGTACCCTTA CAGCATCTGA AATAAAAACT CTTCCTATCC GTTCTCCAAA AGTGTCTAAC 
GTTAAAAAGG CTCTCAGAAT ATATCATGAG CAGCAGGTGA AGTCTCGTGG ACTAGAAGAG 
ATTCCAGTTT TTGATATTTC TGAAAAAACA ATAAATGGAA TGGAAAATAA ATCTATCTCT 
CCTGATGAAG AAAGACTTGC CTCAGATTTA ATTGATCCTG TTGCTTTAGA AGCTCCGTTA 
TCTAAAAATC TTGTGGCACA GATTAGCGCA CTTGCTCTTC AACTAGATTC AGAAGATCTC 
TATAATTATT CAGGAAGCCA GCTATTTGAA ATGCACGAGA AACTTGGTAG CATGGCAAAC 
TCTATAATTA AAAATCTGCA GTCACGTTGG AGGTCACCAG CCCATGAAAA TTCTTTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010800376.1
CDS	171..7490
Translation	MTAADQSPLAPLLETLEDPSASLGEQTDAYLTLTSRMTGEDGKEIITEIEKKLPQLFKVLKTHIASQNSELSSAALQALGFCLYNPKIASGLSEINIQELLSKLNDIVKSSDKNVRTRALWVISKQTFPTEVVGKVVSSIIDSLEIVFNRGEMLSAVVDYEALNVIIRLIEQAPVQMGEESIRWAKLVIPLLVHSAQKVHLRGATALEMGMPLLLQKQQEIASITEQLMTTKLLSELQKLFMSKNETYVLKLWPLFVKLLGKTLHRSGSFINSLLQLEELGFRSGAPMIKKIAFIAWKSLIDNFALNPEILCSAKRLKLLMQPLSSIHVRTETLALTKLEVWWYLLMRLGPHLPANFEQVCVPLIQSTISIDSNASPQGSSSRVATTPGLSPMTPVHKGASPYGTPVTPRMNLNSNFGMATIPSIQLLGLEMLLHFLLGPEVVSFAKQNKLILSLEPLEHPLISSSSFFSKHSNTLITAVHDSFVAVGKDASDVVINAIWKELISLVKSVIESGNKKERPGSEVLTLLLKSLESIVNSEVFPVLKTLVLMEITIKGLPQKVLGSPAYQVANMDILNGTPALFLIQLTFNNNLLECGVEDERFFLNLETLVGCVLSGPTSPLAFSDSVLNVVNQNAKQLDNKEHLWRMWSIIVTPLTELINQTNEVNQGDALEHNFSAIYGALILPINHIFPAQKIPVVTMKTLLKTWSELYRAFARCAALVATAEENLCCEEFCSKIMSSLEDEGLSNLLFLDRITHIITVMVDCIDFSPYNIKYQPKTKSPQRPTDWSKKKKEPLGKLASLIKLIVRVIYSFHTLSLKEVHSDTLLTIGNSITSILSNLLGHISLPSMIQKIFAALTRPLALFYENSKLDEVPKVYSCMNNKLEKLLGDIIACLHVSYTGTYDSELLEQISPLLCIIFSHKNKQIRKQSAQFWNATFAKVTMLIYPEELKPILKQAKQKSLLLLPGLENVEIMEESSGPYSDMTENSQLNMKISGMERKSSGKRDSFLAQTKDTKDSMKPPAKLKLDSSTPKAKGEMPLEEEKSTDFVFIPPEVKETKERILTEHQKEVLKTKRCDIPAMYNNLDVSQDTLFSQYSQEESMEISTLIEKSKEDSKMIFKEEQKENDIVVPQHVMENCGMDEHLEKASLLNNECGSIEETSPKILSSNNDARKKALIASNKPTECASTTENPSGVSSSSVSIATISGAPLQPTSRRQSFITLEKFDGSENRPFSPSPLNNMSSTVIVKSNQEGMGKTDNPSKAKKREVALSKSDSEKITHGTKRSSRRSSKSEPIGSKKSKPLMRSEQEKSTQESVDGTVALENNPPGLLNQTECVLDNQVHLSESTVEFEDTMLESTVENTVLENNTVEERTLEVNLESKENTPPAIITDQTVNEDSHIQVTPNQKTLRRSLRRRSETAEPTTDSQDKENNHQKRERRKEEEKTPQKSPLHVKDDVLPKQKLISEQSVQGNLVDKGNNLHEKAFGETSANAEIDENRRKPDLENTKSEGDGVPDIADKSSEKPVRGRTRYQTRRASQGLLSSIENSESDSSEAKEEGSKKKRSGKWKNKSSDNVDMEDQEEKMVKQECIKIENQAHDYKGNPEVDKDIKSQICEKGDESTTVSLQDSTVSPDLLQVYDDVLNTSEGESKSNKDADHSFSNPSVPESNLRTRNASKRLHKRDSIENSMGESSKIGTSDISLLSEKTLQTVECQHKRSRRVRRSKGCDCCGEKSQSQEKSFTGLKDTENYDVKVSETKKTDGETPVSISETSKADLSSEVKLLDEHHSVNFHLDLKEENDTTNDSLIISETELKENTTQSFLPSEMVKFKETSRRDSEEAISLETQEPSNKKCKTVDPHLDVSKDISLKCFSLETKEEKSEATSKEKLSTENIVIVESNAESNPEVDAMELNVENDQSEAGFSEQKNVKTGIPGKEVTEENSERMDTPEKLKANEAVTEEFNSATDYSDTPTSVKVNAQVEISEQIAVGELGGGSDESDPGSSEEMNAEMKNCEKTAVCEVTTESDHVTETEDEDVTTTASLPIEVETKELDVEVNNFVPDTLEMITEEGKVDPNKTETNTEPNKLEETQLDDNQMVVESNETLPEVHHTSEKVEEIAQPLTSETDISGLISEDSNTSPQKSKDIDPLLMSANESPSGMHTRCVWSPLASPSTSILKRGLKRPQEDEISSPVHKVRRVSFADPIYQAGLADDIDRRCSIVRCHSSNSSLMVKSVKTSPTVQSKIAEMAKESVPCPTESVYPALVNCVAPVEIILPQITSNMWARGLGQLIRAKNIKTIGDLSTLTASEIKTLPIRSPKVSNVKKALRIYHEQQVKSRGLEEIPVFDISEKTINGMENKSISPDEERLASDLIDPVALEAPLSKNLVAQISALALQLDSEDLYNYSGSQLFEMHEKLGSMANSIIKNLQSRWRSPAHENSF

Target ORF information:

RefSeq Version	XM_010802074.3
Organism	Bos taurus(cattle)
Definition	Bos taurus replication timing regulatory factor 1 (RIF1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010802074.3

ORF Insert Sequence:

ATGACGGCCG CGGATCAGAG CCCCCTGGCA CCGCTGTTGG AGACTTTGGA AGACCCTTCC 
GCCTCCCTTG GAGAGCAGAC CGACGCTTAC CTGACCCTGA CCAGTCGTAT GACTGGAGAA 
GATGGAAAAG AAATCATTAC AGAAATTGAG AAAAAACTTC CTCAGCTATT TAAAGTTTTA 
AAGACTCACA TTGCCAGTCA AAACTCAGAG CTCAGTAGTG CTGCTCTACA GGCCTTGGGG 
TTTTGCTTAT ATAATCCCAA AATTGCCTCT GGATTATCAG AGATAAATAT TCAAGAACTG 
CTTTCAAAAC TGAATGATAT TGTTAAAAGT TCAGATAAAA ATGTACGTAC TAGGGCACTT 
TGGGTGATAT CCAAGCAGAC GTTTCCCACT GAAGTCGTTG GCAAAGTAGT TTCTAGTATC 
ATTGACTCAC TAGAAATAGT ATTTAATAGA GGAGAGATGC TTTCTGCTGT TGTGGATTAT 
GAAGCATTAA ATGTCATTAT AAGACTAATT GAACAAGCCC CAGTTCAAAT GGGAGAAGAG 
TCAATTAGGT GGGCAAAATT GGTCATACCT TTACTGGTTC ATTCAGCCCA AAAAGTACAT 
TTGCGGGGAG CCACTGCTCT AGAGATGGGA ATGCCTTTGT TGCTTCAGAA ACAGCAAGAA 
ATAGCATCTA TCACAGAGCA GCTTATGACT ACCAAATTAC TTTCAGAACT CCAGAAACTG 
TTTATGAGTA AAAATGAGAC TTATGTGTTA AAATTGTGGC CTTTATTTGT CAAACTTCTT 
GGGAAGACCT TGCATCGAAG TGGGAGTTTC ATTAATTCTC TGTTACAGCT GGAAGAACTG 
GGATTTCGTA GTGGAGCACC CATGATTAAA AAAATAGCTT TTATTGCTTG GAAGAGTTTA 
ATAGATAATT TTGCTTTAAA TCCAGAAATA CTGTGTAGTG CAAAAAGACT GAAGTTGTTG 
ATGCAGCCTT TGAGTTCTAT CCATGTGAGA ACAGAAACTC TAGCGCTGAC AAAACTAGAA 
GTCTGGTGGT ATTTGTTGAT GAGACTAGGA CCTCATCTTC CTGCTAATTT TGAACAGGTT 
TGTGTGCCTC TGATTCAGAG CACAATAAGC ATTGATTCTA ATGCTTCACC TCAAGGGAGT 
TCATCTCGTG TAGCTACTAC TCCAGGTTTA AGCCCTATGA CCCCAGTACA CAAAGGTGCT 
TCTCCATATG GAACTCCTGT AACCCCTCGG ATGAACTTGA ATTCAAATTT TGGAATGGCC 
ACAATCCCCT CTATACAACT TTTGGGGCTT GAAATGTTGC TTCATTTTTT GTTGGGTCCA 
GAAGTCGTGA GTTTTGCTAA GCAAAACAAA CTTATACTGA GCCTAGAACC ACTTGAACAT 
CCATTAATCA GCAGCTCTTC TTTTTTCTCC AAACACTCAA ATACACTTAT CACTGCTGTT 
CATGATAGCT TTGTTGCAGT TGGAAAAGAT GCTTCTGATG TAGTTATTAA TGCTATTTGG 
AAGGAGTTAA TTAGCTTGGT GAAGTCAGTT ATTGAATCAG GTAACAAAAA AGAGAGACCA 
GGTTCTGAAG TTTTGACTCT CTTATTAAAA TCTTTGGAAA GCATAGTGAA TTCAGAAGTA 
TTTCCTGTAT TGAAAACACT GGTTCTCATG GAAATTACAA TTAAAGGACT TCCTCAGAAA 
GTATTAGGTT CTCCAGCATA TCAGGTTGCT AATATGGATA TTCTTAATGG AACCCCTGCT 
TTGTTCTTAA TTCAGTTAAC TTTCAATAAT AATCTATTGG AATGTGGTGT GGAAGACGAA 
AGGTTTTTTC TCAACCTGGA AACACTTGTG GGCTGTGTTC TTTCGGGTCC AACTTCACCA 
CTCGCTTTCA GTGACTCTGT CTTAAATGTT GTCAATCAAA ATGCAAAGCA GTTGGATAAC 
AAGGAGCATC TTTGGAGAAT GTGGAGTATT ATAGTCACCC CATTAACAGA ATTGATTAAT 
CAGACCAATG AAGTGAATCA AGGGGATGCC TTAGAACATA ATTTCAGTGC TATCTATGGT 
GCACTGATTT TGCCAATAAA CCATATTTTT CCAGCACAAA AAATTCCAGT GGTCACCATG 
AAGACCTTAC TTAAAACTTG GTCAGAATTA TATAGAGCAT TTGCTCGCTG TGCAGCTTTG 
GTGGCAACTG CGGAAGAGAA TTTATGCTGT GAGGAATTTT GTTCCAAGAT AATGTCCAGT 
TTGGAAGATG AAGGCCTTTC AAATTTATTG TTCTTGGATA GGATCACTCA TATTATTACT 
GTAATGGTTG ATTGCATCGA CTTTTCACCA TATAATATTA AATATCAACC CAAAACTAAA 
TCACCACAGA GACCTACAGA TTGGTCCAAA AAGAAGAAGG AACCTTTAGG GAAGTTGGCT 
TCTTTAATTA AACTTATTGT GAGAGTGATC TATTCTTTTC ACACACTGAG CCTCAAGGAA 
GTACATTCTG ATACTCTCCT CACTATTGGC AACTCAATCA CCAGCATTCT TTCCAATTTA 
CTTGGACATA TTTCTCTGCC TTCTATGATC CAGAAAATAT TTGCAGCTTT AACAAGACCT 
CTGGCATTAT TTTATGAAAA CTCAAAGCTT GATGAAGTTC CTAAAGTGTA TAGTTGTATG 
AACAACAAGT TAGAAAAGCT ACTAGGAGAT ATAATTGCTT GTCTACATGT CAGCTACACT 
GGAACTTACG ACAGTGAACT TCTTGAACAG ATCTCACCAT TATTATGCAT AATATTTTCA 
CACAAGAACA AACAGATAAG AAAACAAAGT GCTCAGTTCT GGAATGCCAC ATTTGCTAAA 
GTGACGATGT TGATTTATCC TGAGGAGTTA AAGCCAATAC TGAAACAAGC CAAACAAAAA 
TCTCTGCTTT TATTGCCTGG TTTGGAAAAT GTTGAAATTA TGGAGGAATC CAGTGGACCA 
TATTCAGATA TGACAGAAAA TTCCCAATTA AATATGAAGA TAAGTGGCAT GGAAAGAAAA 
TCAAGTGGAA AAAGAGATTC TTTTTTGGCA CAAACAAAGG ATACAAAAGA CAGTATGAAA 
CCACCAGCTA AATTGAAACT AGATTCTTCA ACTCCAAAAG CAAAGGGTGA AATGCCTTTG 
GAAGAAGAAA AGTCTACTGA CTTTGTGTTT ATACCTCCAG AAGTAAAAGA AACAAAGGAA 
AGAATACTAA CTGAACACCA GAAAGAAGTA CTCAAAACAA AGAGGTGTGA TATTCCTGCC 
ATGTATAATA ATCTGGATGT TTCACAAGAT ACTTTATTTT CTCAGTATAG TCAGGAAGAA 
TCTATGGAAA TTTCTACTTT AATTGAAAAA TCAAAAGAAG ACTCCAAGAT GATATTTAAG 
GAGGAACAAA AGGAGAATGA CATTGTCGTT CCTCAACATG TCATGGAAAA CTGCGGTATG 
GATGAACATC TTGAAAAGGC TTCCCTTTTG AATAATGAGT GTGGTTCTAT TGAGGAAACC 
AGTCCAAAAA TACTGAGCAG TAATAATGAT GCCAGAAAAA AAGCTTTAAT TGCTTCAAAT 
AAACCAACAG AATGTGCATC TACTACAGAA AATCCATCTG GTGTCAGCAG TAGCTCGGTT 
TCTATTGCCA CCATTTCTGG AGCTCCCTTA CAGCCTACAA GTCGAAGGCA ATCCTTTATT 
ACTTTGGAAA AGTTTGATGG TTCAGAAAAT AGACCTTTTA GTCCATCACC CTTGAATAAT 
ATGTCATCAA CTGTAATAGT GAAAAGTAAC CAGGAAGGCA TGGGTAAAAC AGATAATCCA 
TCAAAAGCAA AGAAGAGAGA AGTGGCTCTT TCCAAATCTG ACTCTGAAAA AATAACACAT 
GGGACTAAGA GATCAAGCCG AAGGTCGAGT AAATCTGAAC CAATAGGGAG TAAAAAGTCT 
AAACCCTTAA TGAGATCTGA ACAAGAGAAA AGTACTCAAG AATCTGTTGA TGGCACAGTA 
GCCTTAGAAA ATAACCCACC TGGCTTGCTT AATCAGACAG AATGTGTGTT AGATAATCAG 
GTTCATCTCT CTGAATCTAC AGTGGAGTTT GAGGATACAA TGCTTGAATC AACAGTGGAG 
AATACTGTAT TAGAAAACAA TACTGTAGAA GAGAGAACCT TAGAAGTTAA CTTGGAATCC 
AAAGAAAACA CACCCCCAGC CATAATAACG GATCAGACAG TAAATGAGGA TAGTCATATT 
CAGGTAACTC CAAATCAGAA AACCCTTAGG CGATCTTTAA GGCGACGTTC AGAAACAGCA 
GAACCAACCA CTGATAGCCA AGATAAAGAA AATAATCATC AAAAAAGGGA AAGACGTAAA 
GAAGAAGAAA AGACTCCGCA GAAGAGTCCG TTACATGTAA AAGATGATGT TTTACCTAAG 
CAAAAATTGA TTTCTGAACA AAGTGTACAG GGAAACTTAG TTGACAAAGG AAATAATTTA 
CATGAGAAGG CTTTTGGGGA GACCAGTGCT AATGCTGAAA TTGATGAAAA TAGAAGAAAG 
CCAGATCTTG AGAATACTAA ATCTGAAGGA GATGGTGTGC CGGACATTGC AGATAAATCC 
TCTGAGAAAC CAGTAAGGGG ACGAACACGA TACCAAACAA GAAGAGCATC CCAGGGTTTA 
CTTTCCAGCA TTGAAAACTC AGAATCTGAT AGTTCTGAGG CAAAAGAAGA AGGTTCTAAA 
AAGAAAAGAT CTGGAAAATG GAAGAACAAA AGCAGTGACA ATGTTGACAT GGAAGATCAA 
GAAGAGAAAA TGGTAAAACA GGAATGTATA AAAATTGAAA ATCAGGCACA TGATTATAAA 
GGGAATCCTG AAGTAGACAA AGATATAAAA TCTCAAATCT GTGAAAAAGG AGATGAAAGT 
ACTACTGTAA GTCTTCAAGA TTCTACCGTA TCTCCAGATT TATTACAAGT TTATGATGAT 
GTATTGAATA CTTCTGAGGG AGAAAGTAAA AGTAACAAAG ATGCAGACCA TTCCTTTTCA 
AATCCTTCTG TACCAGAATC AAATCTAAGG ACTAGAAATG CCAGTAAGAG GTTACATAAG 
CGAGATTCTA TAGAAAATAG CATGGGGGAA TCCTCAAAAA TAGGGACATC AGACATTTCT 
TTGCTTTCTG AAAAAACTCT TCAGACAGTA GAATGCCAGC ACAAGAGAAG TAGGAGGGTG 
AGGCGATCTA AAGGTTGTGA TTGTTGTGGA GAAAAATCAC AGTCTCAGGA AAAGTCATTT 
ACTGGGTTAA AGGATACAGA AAATTACGAT GTAAAAGTCA GTGAAACAAA AAAGACAGAT 
GGGGAAACAC CTGTATCTAT TTCAGAAACT TCGAAAGCTG ATCTGTCCTC GGAGGTTAAA 
CTTTTAGATG AGCATCATAG TGTGAATTTT CATTTGGATC TCAAGGAAGA GAATGATACT 
ACTAATGATT CATTAATTAT ATCTGAAACT GAGTTAAAAG AAAATACTAC TCAAAGCTTT 
CTTCCTTCTG AAATGGTAAA ATTTAAAGAA ACTTCTCGTA GGGATTCTGA GGAAGCAATA 
TCTCTAGAAA CCCAGGAGCC ATCTAATAAG AAATGTAAAA CTGTTGACCC ACATTTAGAT 
GTTTCCAAAG ATATTTCACT GAAATGTTTT TCTTTGGAGA CTAAAGAAGA AAAGTCAGAG 
GCCACTTCAA AAGAGAAATT GAGTACAGAG AACATTGTTA TTGTTGAATC AAATGCAGAA 
TCAAATCCAG AAGTAGATGC TATGGAATTG AATGTAGAAA ATGATCAGTC TGAAGCTGGC 
TTCTCTGAAC AAAAGAATGT CAAAACTGGT ATTCCAGGGA AAGAAGTAAC AGAGGAAAAC 
AGTGAAAGAA TGGATACACC TGAAAAACTG AAAGCCAATG AAGCAGTAAC TGAGGAATTT 
AATTCAGCTA CTGATTATTC GGATACTCCG ACATCTGTAA AAGTGAATGC TCAGGTAGAG 
ATCTCTGAAC AAATAGCGGT TGGGGAGCTA GGTGGAGGAA GTGATGAATC TGATCCAGGC 
TCATCTGAAG AAATGAACGC TGAAATGAAA AATTGTGAAA AAACAGCGGT TTGTGAGGTG 
ACTACTGAAT CTGACCACGT CACTGAAACC GAAGACGAAG ATGTAACTAC CACGGCTTCT 
CTGCCTATAG AAGTTGAAAC AAAGGAATTA GATGTAGAAG TCAATAACTT TGTTCCCGAC 
ACACTTGAGA TGATCACTGA AGAAGGAAAG GTTGACCCTA ACAAAACTGA AACGAATACT 
GAACCTAATA AATTGGAGGA AACACAGTTA GATGACAATC AGATGGTAGT GGAAAGTAAT 
GAAACTTTAC CGGAAGTTCA CCATACTTCA GAGAAAGTAG AGGAGATAGC ACAACCTCTG 
ACATCTGAAA CAGATATTTC TGGACTAATA TCAGAAGACA GTAATACATC TCCTCAAAAA 
TCGAAGGATA TTGATCCTTT ACTCATGTCA GCAAACGAGA GTCCTAGTGG CATGCACACA 
CGTTGTGTGT GGTCTCCTTT GGCTTCTCCA TCCACCAGCA TTTTAAAGAG AGGACTAAAA 
AGACCCCAAG AAGATGAAAT CTCGTCACCT GTCCACAAGG TTCGCCGTGT CTCCTTTGCA 
GATCCTATAT ACCAAGCAGG ATTGGCAGAT GACATTGATA GGCGATGCTC TATTGTTAGG 
TGCCATTCTT CAAATAGTTC TCTCATGGTA AAAAGTGTTA AAACTTCACC TACTGTGCAA 
TCTAAGATTG CAGAAATGGC TAAGGAATCT GTGCCATGCC CAACAGAAAG TGTTTACCCT 
GCTTTGGTGA ACTGTGTAGC ACCAGTTGAA ATCATTTTAC CTCAGATTAC ATCCAACATG 
TGGGCAAGAG GACTGGGACA GCTCATTAGA GCCAAGAATA TAAAAACAAT TGGCGATTTG 
AGTACCCTTA CAGCATCTGA AATAAAAACT CTTCCTATCC GTTCTCCAAA AGTGTCTAAC 
GTTAAAAAGG CTCTCAGAAT ATATCATGAG CAGCAGGTGA AGTCTCGTGG ACTAGAAGAG 
ATTCCAGTTT TTGATATTTC TGAAAAAACA ATAAATGGAA TGGAAAATAA ATCTATCTCT 
CCTGATGAAG AAAGACTTGC CTCAGATTTA ATTGATCCTG TTGCTTTAGA AGCTCCGTTA 
TCTAAAAATC TTGTGGCACA GATTAGCGCA CTTGCTCTTC AACTAGATTC AGAAGATCTC 
TATAATTATT CAGGAAGCCA GCTATTTGAA ATGCACGAGA AACTTGGTAG CATGGCAAAC 
TCTATAATTA AAAATCTGCA GTCACGTTGG AGGTCACCAG CCCATGAAAA TTCTTTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RIF1 ( XM_010802074.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010800376.1 Bos taurus replication timing regulatory factor 1 (RIF1), transcript variant X3, mRNA.

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CloneID	OBa55066
Clone ID Related Accession (Same CDS sequence)	XM_010802074.3 , XM_010802074.2 , XM_010826490.2
Accession Version	XM_010802074.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7320bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	telomere-associated protein RIF1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103838.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010802074.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGCCG CGGATCAGAG CCCCCTGGCA CCGCTGTTGG AGACTTTGGA AGACCCTTCC 
GCCTCCCTTG GAGAGCAGAC CGACGCTTAC CTGACCCTGA CCAGTCGTAT GACTGGAGAA 
GATGGAAAAG AAATCATTAC AGAAATTGAG AAAAAACTTC CTCAGCTATT TAAAGTTTTA 
AAGACTCACA TTGCCAGTCA AAACTCAGAG CTCAGTAGTG CTGCTCTACA GGCCTTGGGG 
TTTTGCTTAT ATAATCCCAA AATTGCCTCT GGATTATCAG AGATAAATAT TCAAGAACTG 
CTTTCAAAAC TGAATGATAT TGTTAAAAGT TCAGATAAAA ATGTACGTAC TAGGGCACTT 
TGGGTGATAT CCAAGCAGAC GTTTCCCACT GAAGTCGTTG GCAAAGTAGT TTCTAGTATC 
ATTGACTCAC TAGAAATAGT ATTTAATAGA GGAGAGATGC TTTCTGCTGT TGTGGATTAT 
GAAGCATTAA ATGTCATTAT AAGACTAATT GAACAAGCCC CAGTTCAAAT GGGAGAAGAG 
TCAATTAGGT GGGCAAAATT GGTCATACCT TTACTGGTTC ATTCAGCCCA AAAAGTACAT 
TTGCGGGGAG CCACTGCTCT AGAGATGGGA ATGCCTTTGT TGCTTCAGAA ACAGCAAGAA 
ATAGCATCTA TCACAGAGCA GCTTATGACT ACCAAATTAC TTTCAGAACT CCAGAAACTG 
TTTATGAGTA AAAATGAGAC TTATGTGTTA AAATTGTGGC CTTTATTTGT CAAACTTCTT 
GGGAAGACCT TGCATCGAAG TGGGAGTTTC ATTAATTCTC TGTTACAGCT GGAAGAACTG 
GGATTTCGTA GTGGAGCACC CATGATTAAA AAAATAGCTT TTATTGCTTG GAAGAGTTTA 
ATAGATAATT TTGCTTTAAA TCCAGAAATA CTGTGTAGTG CAAAAAGACT GAAGTTGTTG 
ATGCAGCCTT TGAGTTCTAT CCATGTGAGA ACAGAAACTC TAGCGCTGAC AAAACTAGAA 
GTCTGGTGGT ATTTGTTGAT GAGACTAGGA CCTCATCTTC CTGCTAATTT TGAACAGGTT 
TGTGTGCCTC TGATTCAGAG CACAATAAGC ATTGATTCTA ATGCTTCACC TCAAGGGAGT 
TCATCTCGTG TAGCTACTAC TCCAGGTTTA AGCCCTATGA CCCCAGTACA CAAAGGTGCT 
TCTCCATATG GAACTCCTGT AACCCCTCGG ATGAACTTGA ATTCAAATTT TGGAATGGCC 
ACAATCCCCT CTATACAACT TTTGGGGCTT GAAATGTTGC TTCATTTTTT GTTGGGTCCA 
GAAGTCGTGA GTTTTGCTAA GCAAAACAAA CTTATACTGA GCCTAGAACC ACTTGAACAT 
CCATTAATCA GCAGCTCTTC TTTTTTCTCC AAACACTCAA ATACACTTAT CACTGCTGTT 
CATGATAGCT TTGTTGCAGT TGGAAAAGAT GCTTCTGATG TAGTTATTAA TGCTATTTGG 
AAGGAGTTAA TTAGCTTGGT GAAGTCAGTT ATTGAATCAG GTAACAAAAA AGAGAGACCA 
GGTTCTGAAG TTTTGACTCT CTTATTAAAA TCTTTGGAAA GCATAGTGAA TTCAGAAGTA 
TTTCCTGTAT TGAAAACACT GGTTCTCATG GAAATTACAA TTAAAGGACT TCCTCAGAAA 
GTATTAGGTT CTCCAGCATA TCAGGTTGCT AATATGGATA TTCTTAATGG AACCCCTGCT 
TTGTTCTTAA TTCAGTTAAC TTTCAATAAT AATCTATTGG AATGTGGTGT GGAAGACGAA 
AGGTTTTTTC TCAACCTGGA AACACTTGTG GGCTGTGTTC TTTCGGGTCC AACTTCACCA 
CTCGCTTTCA GTGACTCTGT CTTAAATGTT GTCAATCAAA ATGCAAAGCA GTTGGATAAC 
AAGGAGCATC TTTGGAGAAT GTGGAGTATT ATAGTCACCC CATTAACAGA ATTGATTAAT 
CAGACCAATG AAGTGAATCA AGGGGATGCC TTAGAACATA ATTTCAGTGC TATCTATGGT 
GCACTGATTT TGCCAATAAA CCATATTTTT CCAGCACAAA AAATTCCAGT GGTCACCATG 
AAGACCTTAC TTAAAACTTG GTCAGAATTA TATAGAGCAT TTGCTCGCTG TGCAGCTTTG 
GTGGCAACTG CGGAAGAGAA TTTATGCTGT GAGGAATTTT GTTCCAAGAT AATGTCCAGT 
TTGGAAGATG AAGGCCTTTC AAATTTATTG TTCTTGGATA GGATCACTCA TATTATTACT 
GTAATGGTTG ATTGCATCGA CTTTTCACCA TATAATATTA AATATCAACC CAAAACTAAA 
TCACCACAGA GACCTACAGA TTGGTCCAAA AAGAAGAAGG AACCTTTAGG GAAGTTGGCT 
TCTTTAATTA AACTTATTGT GAGAGTGATC TATTCTTTTC ACACACTGAG CCTCAAGGAA 
GTACATTCTG ATACTCTCCT CACTATTGGC AACTCAATCA CCAGCATTCT TTCCAATTTA 
CTTGGACATA TTTCTCTGCC TTCTATGATC CAGAAAATAT TTGCAGCTTT AACAAGACCT 
CTGGCATTAT TTTATGAAAA CTCAAAGCTT GATGAAGTTC CTAAAGTGTA TAGTTGTATG 
AACAACAAGT TAGAAAAGCT ACTAGGAGAT ATAATTGCTT GTCTACATGT CAGCTACACT 
GGAACTTACG ACAGTGAACT TCTTGAACAG ATCTCACCAT TATTATGCAT AATATTTTCA 
CACAAGAACA AACAGATAAG AAAACAAAGT GCTCAGTTCT GGAATGCCAC ATTTGCTAAA 
GTGACGATGT TGATTTATCC TGAGGAGTTA AAGCCAATAC TGAAACAAGC CAAACAAAAA 
TCTCTGCTTT TATTGCCTGG TTTGGAAAAT GTTGAAATTA TGGAGGAATC CAGTGGACCA 
TATTCAGATA TGACAGAAAA TTCCCAATTA AATATGAAGA TAAGTGGCAT GGAAAGAAAA 
TCAAGTGGAA AAAGAGATTC TTTTTTGGCA CAAACAAAGG ATACAAAAGA CAGTATGAAA 
CCACCAGCTA AATTGAAACT AGATTCTTCA ACTCCAAAAG CAAAGGGTGA AATGCCTTTG 
GAAGAAGAAA AGTCTACTGA CTTTGTGTTT ATACCTCCAG AAGTAAAAGA AACAAAGGAA 
AGAATACTAA CTGAACACCA GAAAGAAGTA CTCAAAACAA AGAGGTGTGA TATTCCTGCC 
ATGTATAATA ATCTGGATGT TTCACAAGAT ACTTTATTTT CTCAGTATAG TCAGGAAGAA 
TCTATGGAAA TTTCTACTTT AATTGAAAAA TCAAAAGAAG ACTCCAAGAT GATATTTAAG 
GAGGAACAAA AGGAGAATGA CATTGTCGTT CCTCAACATG TCATGGAAAA CTGCGGTATG 
GATGAACATC TTGAAAAGGC TTCCCTTTTG AATAATGAGT GTGGTTCTAT TGAGGAAACC 
AGTCCAAAAA TACTGAGCAG TAATAATGAT GCCAGAAAAA AAGCTTTAAT TGCTTCAAAT 
AAACCAACAG AATGTGCATC TACTACAGAA AATCCATCTG GTGTCAGCAG TAGCTCGGTT 
TCTATTGCCA CCATTTCTGG AGCTCCCTTA CAGCCTACAA GTCGAAGGCA ATCCTTTATT 
ACTTTGGAAA AGTTTGATGG TTCAGAAAAT AGACCTTTTA GTCCATCACC CTTGAATAAT 
ATGTCATCAA CTGTAATAGT GAAAAGTAAC CAGGAAGGCA TGGGTAAAAC AGATAATCCA 
TCAAAAGCAA AGAAGAGAGA AGTGGCTCTT TCCAAATCTG ACTCTGAAAA AATAACACAT 
GGGACTAAGA GATCAAGCCG AAGGTCGAGT AAATCTGAAC CAATAGGGAG TAAAAAGTCT 
AAACCCTTAA TGAGATCTGA ACAAGAGAAA AGTACTCAAG AATCTGTTGA TGGCACAGTA 
GCCTTAGAAA ATAACCCACC TGGCTTGCTT AATCAGACAG AATGTGTGTT AGATAATCAG 
GTTCATCTCT CTGAATCTAC AGTGGAGTTT GAGGATACAA TGCTTGAATC AACAGTGGAG 
AATACTGTAT TAGAAAACAA TACTGTAGAA GAGAGAACCT TAGAAGTTAA CTTGGAATCC 
AAAGAAAACA CACCCCCAGC CATAATAACG GATCAGACAG TAAATGAGGA TAGTCATATT 
CAGGTAACTC CAAATCAGAA AACCCTTAGG CGATCTTTAA GGCGACGTTC AGAAACAGCA 
GAACCAACCA CTGATAGCCA AGATAAAGAA AATAATCATC AAAAAAGGGA AAGACGTAAA 
GAAGAAGAAA AGACTCCGCA GAAGAGTCCG TTACATGTAA AAGATGATGT TTTACCTAAG 
CAAAAATTGA TTTCTGAACA AAGTGTACAG GGAAACTTAG TTGACAAAGG AAATAATTTA 
CATGAGAAGG CTTTTGGGGA GACCAGTGCT AATGCTGAAA TTGATGAAAA TAGAAGAAAG 
CCAGATCTTG AGAATACTAA ATCTGAAGGA GATGGTGTGC CGGACATTGC AGATAAATCC 
TCTGAGAAAC CAGTAAGGGG ACGAACACGA TACCAAACAA GAAGAGCATC CCAGGGTTTA 
CTTTCCAGCA TTGAAAACTC AGAATCTGAT AGTTCTGAGG CAAAAGAAGA AGGTTCTAAA 
AAGAAAAGAT CTGGAAAATG GAAGAACAAA AGCAGTGACA ATGTTGACAT GGAAGATCAA 
GAAGAGAAAA TGGTAAAACA GGAATGTATA AAAATTGAAA ATCAGGCACA TGATTATAAA 
GGGAATCCTG AAGTAGACAA AGATATAAAA TCTCAAATCT GTGAAAAAGG AGATGAAAGT 
ACTACTGTAA GTCTTCAAGA TTCTACCGTA TCTCCAGATT TATTACAAGT TTATGATGAT 
GTATTGAATA CTTCTGAGGG AGAAAGTAAA AGTAACAAAG ATGCAGACCA TTCCTTTTCA 
AATCCTTCTG TACCAGAATC AAATCTAAGG ACTAGAAATG CCAGTAAGAG GTTACATAAG 
CGAGATTCTA TAGAAAATAG CATGGGGGAA TCCTCAAAAA TAGGGACATC AGACATTTCT 
TTGCTTTCTG AAAAAACTCT TCAGACAGTA GAATGCCAGC ACAAGAGAAG TAGGAGGGTG 
AGGCGATCTA AAGGTTGTGA TTGTTGTGGA GAAAAATCAC AGTCTCAGGA AAAGTCATTT 
ACTGGGTTAA AGGATACAGA AAATTACGAT GTAAAAGTCA GTGAAACAAA AAAGACAGAT 
GGGGAAACAC CTGTATCTAT TTCAGAAACT TCGAAAGCTG ATCTGTCCTC GGAGGTTAAA 
CTTTTAGATG AGCATCATAG TGTGAATTTT CATTTGGATC TCAAGGAAGA GAATGATACT 
ACTAATGATT CATTAATTAT ATCTGAAACT GAGTTAAAAG AAAATACTAC TCAAAGCTTT 
CTTCCTTCTG AAATGGTAAA ATTTAAAGAA ACTTCTCGTA GGGATTCTGA GGAAGCAATA 
TCTCTAGAAA CCCAGGAGCC ATCTAATAAG AAATGTAAAA CTGTTGACCC ACATTTAGAT 
GTTTCCAAAG ATATTTCACT GAAATGTTTT TCTTTGGAGA CTAAAGAAGA AAAGTCAGAG 
GCCACTTCAA AAGAGAAATT GAGTACAGAG AACATTGTTA TTGTTGAATC AAATGCAGAA 
TCAAATCCAG AAGTAGATGC TATGGAATTG AATGTAGAAA ATGATCAGTC TGAAGCTGGC 
TTCTCTGAAC AAAAGAATGT CAAAACTGGT ATTCCAGGGA AAGAAGTAAC AGAGGAAAAC 
AGTGAAAGAA TGGATACACC TGAAAAACTG AAAGCCAATG AAGCAGTAAC TGAGGAATTT 
AATTCAGCTA CTGATTATTC GGATACTCCG ACATCTGTAA AAGTGAATGC TCAGGTAGAG 
ATCTCTGAAC AAATAGCGGT TGGGGAGCTA GGTGGAGGAA GTGATGAATC TGATCCAGGC 
TCATCTGAAG AAATGAACGC TGAAATGAAA AATTGTGAAA AAACAGCGGT TTGTGAGGTG 
ACTACTGAAT CTGACCACGT CACTGAAACC GAAGACGAAG ATGTAACTAC CACGGCTTCT 
CTGCCTATAG AAGTTGAAAC AAAGGAATTA GATGTAGAAG TCAATAACTT TGTTCCCGAC 
ACACTTGAGA TGATCACTGA AGAAGGAAAG GTTGACCCTA ACAAAACTGA AACGAATACT 
GAACCTAATA AATTGGAGGA AACACAGTTA GATGACAATC AGATGGTAGT GGAAAGTAAT 
GAAACTTTAC CGGAAGTTCA CCATACTTCA GAGAAAGTAG AGGAGATAGC ACAACCTCTG 
ACATCTGAAA CAGATATTTC TGGACTAATA TCAGAAGACA GTAATACATC TCCTCAAAAA 
TCGAAGGATA TTGATCCTTT ACTCATGTCA GCAAACGAGA GTCCTAGTGG CATGCACACA 
CGTTGTGTGT GGTCTCCTTT GGCTTCTCCA TCCACCAGCA TTTTAAAGAG AGGACTAAAA 
AGACCCCAAG AAGATGAAAT CTCGTCACCT GTCCACAAGG TTCGCCGTGT CTCCTTTGCA 
GATCCTATAT ACCAAGCAGG ATTGGCAGAT GACATTGATA GGCGATGCTC TATTGTTAGG 
TGCCATTCTT CAAATAGTTC TCTCATGGTA AAAAGTGTTA AAACTTCACC TACTGTGCAA 
TCTAAGATTG CAGAAATGGC TAAGGAATCT GTGCCATGCC CAACAGAAAG TGTTTACCCT 
GCTTTGGTGA ACTGTGTAGC ACCAGTTGAA ATCATTTTAC CTCAGATTAC ATCCAACATG 
TGGGCAAGAG GACTGGGACA GCTCATTAGA GCCAAGAATA TAAAAACAAT TGGCGATTTG 
AGTACCCTTA CAGCATCTGA AATAAAAACT CTTCCTATCC GTTCTCCAAA AGTGTCTAAC 
GTTAAAAAGG CTCTCAGAAT ATATCATGAG CAGCAGGTGA AGTCTCGTGG ACTAGAAGAG 
ATTCCAGTTT TTGATATTTC TGAAAAAACA ATAAATGGAA TGGAAAATAA ATCTATCTCT 
CCTGATGAAG AAAGACTTGC CTCAGATTTA ATTGATCCTG TTGCTTTAGA AGCTCCGTTA 
TCTAAAAATC TTGTGGCACA GATTAGCGCA CTTGCTCTTC AACTAGATTC AGAAGATCTC 
TATAATTATT CAGGAAGCCA GCTATTTGAA ATGCACGAGA AACTTGGTAG CATGGCAAAC 
TCTATAATTA AAAATCTGCA GTCACGTTGG AGGTCACCAG CCCATGAAAA TTCTTTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010800376.1
CDS	146..7465
Translation	MTAADQSPLAPLLETLEDPSASLGEQTDAYLTLTSRMTGEDGKEIITEIEKKLPQLFKVLKTHIASQNSELSSAALQALGFCLYNPKIASGLSEINIQELLSKLNDIVKSSDKNVRTRALWVISKQTFPTEVVGKVVSSIIDSLEIVFNRGEMLSAVVDYEALNVIIRLIEQAPVQMGEESIRWAKLVIPLLVHSAQKVHLRGATALEMGMPLLLQKQQEIASITEQLMTTKLLSELQKLFMSKNETYVLKLWPLFVKLLGKTLHRSGSFINSLLQLEELGFRSGAPMIKKIAFIAWKSLIDNFALNPEILCSAKRLKLLMQPLSSIHVRTETLALTKLEVWWYLLMRLGPHLPANFEQVCVPLIQSTISIDSNASPQGSSSRVATTPGLSPMTPVHKGASPYGTPVTPRMNLNSNFGMATIPSIQLLGLEMLLHFLLGPEVVSFAKQNKLILSLEPLEHPLISSSSFFSKHSNTLITAVHDSFVAVGKDASDVVINAIWKELISLVKSVIESGNKKERPGSEVLTLLLKSLESIVNSEVFPVLKTLVLMEITIKGLPQKVLGSPAYQVANMDILNGTPALFLIQLTFNNNLLECGVEDERFFLNLETLVGCVLSGPTSPLAFSDSVLNVVNQNAKQLDNKEHLWRMWSIIVTPLTELINQTNEVNQGDALEHNFSAIYGALILPINHIFPAQKIPVVTMKTLLKTWSELYRAFARCAALVATAEENLCCEEFCSKIMSSLEDEGLSNLLFLDRITHIITVMVDCIDFSPYNIKYQPKTKSPQRPTDWSKKKKEPLGKLASLIKLIVRVIYSFHTLSLKEVHSDTLLTIGNSITSILSNLLGHISLPSMIQKIFAALTRPLALFYENSKLDEVPKVYSCMNNKLEKLLGDIIACLHVSYTGTYDSELLEQISPLLCIIFSHKNKQIRKQSAQFWNATFAKVTMLIYPEELKPILKQAKQKSLLLLPGLENVEIMEESSGPYSDMTENSQLNMKISGMERKSSGKRDSFLAQTKDTKDSMKPPAKLKLDSSTPKAKGEMPLEEEKSTDFVFIPPEVKETKERILTEHQKEVLKTKRCDIPAMYNNLDVSQDTLFSQYSQEESMEISTLIEKSKEDSKMIFKEEQKENDIVVPQHVMENCGMDEHLEKASLLNNECGSIEETSPKILSSNNDARKKALIASNKPTECASTTENPSGVSSSSVSIATISGAPLQPTSRRQSFITLEKFDGSENRPFSPSPLNNMSSTVIVKSNQEGMGKTDNPSKAKKREVALSKSDSEKITHGTKRSSRRSSKSEPIGSKKSKPLMRSEQEKSTQESVDGTVALENNPPGLLNQTECVLDNQVHLSESTVEFEDTMLESTVENTVLENNTVEERTLEVNLESKENTPPAIITDQTVNEDSHIQVTPNQKTLRRSLRRRSETAEPTTDSQDKENNHQKRERRKEEEKTPQKSPLHVKDDVLPKQKLISEQSVQGNLVDKGNNLHEKAFGETSANAEIDENRRKPDLENTKSEGDGVPDIADKSSEKPVRGRTRYQTRRASQGLLSSIENSESDSSEAKEEGSKKKRSGKWKNKSSDNVDMEDQEEKMVKQECIKIENQAHDYKGNPEVDKDIKSQICEKGDESTTVSLQDSTVSPDLLQVYDDVLNTSEGESKSNKDADHSFSNPSVPESNLRTRNASKRLHKRDSIENSMGESSKIGTSDISLLSEKTLQTVECQHKRSRRVRRSKGCDCCGEKSQSQEKSFTGLKDTENYDVKVSETKKTDGETPVSISETSKADLSSEVKLLDEHHSVNFHLDLKEENDTTNDSLIISETELKENTTQSFLPSEMVKFKETSRRDSEEAISLETQEPSNKKCKTVDPHLDVSKDISLKCFSLETKEEKSEATSKEKLSTENIVIVESNAESNPEVDAMELNVENDQSEAGFSEQKNVKTGIPGKEVTEENSERMDTPEKLKANEAVTEEFNSATDYSDTPTSVKVNAQVEISEQIAVGELGGGSDESDPGSSEEMNAEMKNCEKTAVCEVTTESDHVTETEDEDVTTTASLPIEVETKELDVEVNNFVPDTLEMITEEGKVDPNKTETNTEPNKLEETQLDDNQMVVESNETLPEVHHTSEKVEEIAQPLTSETDISGLISEDSNTSPQKSKDIDPLLMSANESPSGMHTRCVWSPLASPSTSILKRGLKRPQEDEISSPVHKVRRVSFADPIYQAGLADDIDRRCSIVRCHSSNSSLMVKSVKTSPTVQSKIAEMAKESVPCPTESVYPALVNCVAPVEIILPQITSNMWARGLGQLIRAKNIKTIGDLSTLTASEIKTLPIRSPKVSNVKKALRIYHEQQVKSRGLEEIPVFDISEKTINGMENKSISPDEERLASDLIDPVALEAPLSKNLVAQISALALQLDSEDLYNYSGSQLFEMHEKLGSMANSIIKNLQSRWRSPAHENSF

Target ORF information:

RefSeq Version	XM_010802074.2
Organism	Bos taurus(cattle)
Definition	Bos taurus replication timing regulatory factor 1 (RIF1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010802074.2

ORF Insert Sequence:

ATGACGGCCG CGGATCAGAG CCCCCTGGCA CCGCTGTTGG AGACTTTGGA AGACCCTTCC 
GCCTCCCTTG GAGAGCAGAC CGACGCTTAC CTGACCCTGA CCAGTCGTAT GACTGGAGAA 
GATGGAAAAG AAATCATTAC AGAAATTGAG AAAAAACTTC CTCAGCTATT TAAAGTTTTA 
AAGACTCACA TTGCCAGTCA AAACTCAGAG CTCAGTAGTG CTGCTCTACA GGCCTTGGGG 
TTTTGCTTAT ATAATCCCAA AATTGCCTCT GGATTATCAG AGATAAATAT TCAAGAACTG 
CTTTCAAAAC TGAATGATAT TGTTAAAAGT TCAGATAAAA ATGTACGTAC TAGGGCACTT 
TGGGTGATAT CCAAGCAGAC GTTTCCCACT GAAGTCGTTG GCAAAGTAGT TTCTAGTATC 
ATTGACTCAC TAGAAATAGT ATTTAATAGA GGAGAGATGC TTTCTGCTGT TGTGGATTAT 
GAAGCATTAA ATGTCATTAT AAGACTAATT GAACAAGCCC CAGTTCAAAT GGGAGAAGAG 
TCAATTAGGT GGGCAAAATT GGTCATACCT TTACTGGTTC ATTCAGCCCA AAAAGTACAT 
TTGCGGGGAG CCACTGCTCT AGAGATGGGA ATGCCTTTGT TGCTTCAGAA ACAGCAAGAA 
ATAGCATCTA TCACAGAGCA GCTTATGACT ACCAAATTAC TTTCAGAACT CCAGAAACTG 
TTTATGAGTA AAAATGAGAC TTATGTGTTA AAATTGTGGC CTTTATTTGT CAAACTTCTT 
GGGAAGACCT TGCATCGAAG TGGGAGTTTC ATTAATTCTC TGTTACAGCT GGAAGAACTG 
GGATTTCGTA GTGGAGCACC CATGATTAAA AAAATAGCTT TTATTGCTTG GAAGAGTTTA 
ATAGATAATT TTGCTTTAAA TCCAGAAATA CTGTGTAGTG CAAAAAGACT GAAGTTGTTG 
ATGCAGCCTT TGAGTTCTAT CCATGTGAGA ACAGAAACTC TAGCGCTGAC AAAACTAGAA 
GTCTGGTGGT ATTTGTTGAT GAGACTAGGA CCTCATCTTC CTGCTAATTT TGAACAGGTT 
TGTGTGCCTC TGATTCAGAG CACAATAAGC ATTGATTCTA ATGCTTCACC TCAAGGGAGT 
TCATCTCGTG TAGCTACTAC TCCAGGTTTA AGCCCTATGA CCCCAGTACA CAAAGGTGCT 
TCTCCATATG GAACTCCTGT AACCCCTCGG ATGAACTTGA ATTCAAATTT TGGAATGGCC 
ACAATCCCCT CTATACAACT TTTGGGGCTT GAAATGTTGC TTCATTTTTT GTTGGGTCCA 
GAAGTCGTGA GTTTTGCTAA GCAAAACAAA CTTATACTGA GCCTAGAACC ACTTGAACAT 
CCATTAATCA GCAGCTCTTC TTTTTTCTCC AAACACTCAA ATACACTTAT CACTGCTGTT 
CATGATAGCT TTGTTGCAGT TGGAAAAGAT GCTTCTGATG TAGTTATTAA TGCTATTTGG 
AAGGAGTTAA TTAGCTTGGT GAAGTCAGTT ATTGAATCAG GTAACAAAAA AGAGAGACCA 
GGTTCTGAAG TTTTGACTCT CTTATTAAAA TCTTTGGAAA GCATAGTGAA TTCAGAAGTA 
TTTCCTGTAT TGAAAACACT GGTTCTCATG GAAATTACAA TTAAAGGACT TCCTCAGAAA 
GTATTAGGTT CTCCAGCATA TCAGGTTGCT AATATGGATA TTCTTAATGG AACCCCTGCT 
TTGTTCTTAA TTCAGTTAAC TTTCAATAAT AATCTATTGG AATGTGGTGT GGAAGACGAA 
AGGTTTTTTC TCAACCTGGA AACACTTGTG GGCTGTGTTC TTTCGGGTCC AACTTCACCA 
CTCGCTTTCA GTGACTCTGT CTTAAATGTT GTCAATCAAA ATGCAAAGCA GTTGGATAAC 
AAGGAGCATC TTTGGAGAAT GTGGAGTATT ATAGTCACCC CATTAACAGA ATTGATTAAT 
CAGACCAATG AAGTGAATCA AGGGGATGCC TTAGAACATA ATTTCAGTGC TATCTATGGT 
GCACTGATTT TGCCAATAAA CCATATTTTT CCAGCACAAA AAATTCCAGT GGTCACCATG 
AAGACCTTAC TTAAAACTTG GTCAGAATTA TATAGAGCAT TTGCTCGCTG TGCAGCTTTG 
GTGGCAACTG CGGAAGAGAA TTTATGCTGT GAGGAATTTT GTTCCAAGAT AATGTCCAGT 
TTGGAAGATG AAGGCCTTTC AAATTTATTG TTCTTGGATA GGATCACTCA TATTATTACT 
GTAATGGTTG ATTGCATCGA CTTTTCACCA TATAATATTA AATATCAACC CAAAACTAAA 
TCACCACAGA GACCTACAGA TTGGTCCAAA AAGAAGAAGG AACCTTTAGG GAAGTTGGCT 
TCTTTAATTA AACTTATTGT GAGAGTGATC TATTCTTTTC ACACACTGAG CCTCAAGGAA 
GTACATTCTG ATACTCTCCT CACTATTGGC AACTCAATCA CCAGCATTCT TTCCAATTTA 
CTTGGACATA TTTCTCTGCC TTCTATGATC CAGAAAATAT TTGCAGCTTT AACAAGACCT 
CTGGCATTAT TTTATGAAAA CTCAAAGCTT GATGAAGTTC CTAAAGTGTA TAGTTGTATG 
AACAACAAGT TAGAAAAGCT ACTAGGAGAT ATAATTGCTT GTCTACATGT CAGCTACACT 
GGAACTTACG ACAGTGAACT TCTTGAACAG ATCTCACCAT TATTATGCAT AATATTTTCA 
CACAAGAACA AACAGATAAG AAAACAAAGT GCTCAGTTCT GGAATGCCAC ATTTGCTAAA 
GTGACGATGT TGATTTATCC TGAGGAGTTA AAGCCAATAC TGAAACAAGC CAAACAAAAA 
TCTCTGCTTT TATTGCCTGG TTTGGAAAAT GTTGAAATTA TGGAGGAATC CAGTGGACCA 
TATTCAGATA TGACAGAAAA TTCCCAATTA AATATGAAGA TAAGTGGCAT GGAAAGAAAA 
TCAAGTGGAA AAAGAGATTC TTTTTTGGCA CAAACAAAGG ATACAAAAGA CAGTATGAAA 
CCACCAGCTA AATTGAAACT AGATTCTTCA ACTCCAAAAG CAAAGGGTGA AATGCCTTTG 
GAAGAAGAAA AGTCTACTGA CTTTGTGTTT ATACCTCCAG AAGTAAAAGA AACAAAGGAA 
AGAATACTAA CTGAACACCA GAAAGAAGTA CTCAAAACAA AGAGGTGTGA TATTCCTGCC 
ATGTATAATA ATCTGGATGT TTCACAAGAT ACTTTATTTT CTCAGTATAG TCAGGAAGAA 
TCTATGGAAA TTTCTACTTT AATTGAAAAA TCAAAAGAAG ACTCCAAGAT GATATTTAAG 
GAGGAACAAA AGGAGAATGA CATTGTCGTT CCTCAACATG TCATGGAAAA CTGCGGTATG 
GATGAACATC TTGAAAAGGC TTCCCTTTTG AATAATGAGT GTGGTTCTAT TGAGGAAACC 
AGTCCAAAAA TACTGAGCAG TAATAATGAT GCCAGAAAAA AAGCTTTAAT TGCTTCAAAT 
AAACCAACAG AATGTGCATC TACTACAGAA AATCCATCTG GTGTCAGCAG TAGCTCGGTT 
TCTATTGCCA CCATTTCTGG AGCTCCCTTA CAGCCTACAA GTCGAAGGCA ATCCTTTATT 
ACTTTGGAAA AGTTTGATGG TTCAGAAAAT AGACCTTTTA GTCCATCACC CTTGAATAAT 
ATGTCATCAA CTGTAATAGT GAAAAGTAAC CAGGAAGGCA TGGGTAAAAC AGATAATCCA 
TCAAAAGCAA AGAAGAGAGA AGTGGCTCTT TCCAAATCTG ACTCTGAAAA AATAACACAT 
GGGACTAAGA GATCAAGCCG AAGGTCGAGT AAATCTGAAC CAATAGGGAG TAAAAAGTCT 
AAACCCTTAA TGAGATCTGA ACAAGAGAAA AGTACTCAAG AATCTGTTGA TGGCACAGTA 
GCCTTAGAAA ATAACCCACC TGGCTTGCTT AATCAGACAG AATGTGTGTT AGATAATCAG 
GTTCATCTCT CTGAATCTAC AGTGGAGTTT GAGGATACAA TGCTTGAATC AACAGTGGAG 
AATACTGTAT TAGAAAACAA TACTGTAGAA GAGAGAACCT TAGAAGTTAA CTTGGAATCC 
AAAGAAAACA CACCCCCAGC CATAATAACG GATCAGACAG TAAATGAGGA TAGTCATATT 
CAGGTAACTC CAAATCAGAA AACCCTTAGG CGATCTTTAA GGCGACGTTC AGAAACAGCA 
GAACCAACCA CTGATAGCCA AGATAAAGAA AATAATCATC AAAAAAGGGA AAGACGTAAA 
GAAGAAGAAA AGACTCCGCA GAAGAGTCCG TTACATGTAA AAGATGATGT TTTACCTAAG 
CAAAAATTGA TTTCTGAACA AAGTGTACAG GGAAACTTAG TTGACAAAGG AAATAATTTA 
CATGAGAAGG CTTTTGGGGA GACCAGTGCT AATGCTGAAA TTGATGAAAA TAGAAGAAAG 
CCAGATCTTG AGAATACTAA ATCTGAAGGA GATGGTGTGC CGGACATTGC AGATAAATCC 
TCTGAGAAAC CAGTAAGGGG ACGAACACGA TACCAAACAA GAAGAGCATC CCAGGGTTTA 
CTTTCCAGCA TTGAAAACTC AGAATCTGAT AGTTCTGAGG CAAAAGAAGA AGGTTCTAAA 
AAGAAAAGAT CTGGAAAATG GAAGAACAAA AGCAGTGACA ATGTTGACAT GGAAGATCAA 
GAAGAGAAAA TGGTAAAACA GGAATGTATA AAAATTGAAA ATCAGGCACA TGATTATAAA 
GGGAATCCTG AAGTAGACAA AGATATAAAA TCTCAAATCT GTGAAAAAGG AGATGAAAGT 
ACTACTGTAA GTCTTCAAGA TTCTACCGTA TCTCCAGATT TATTACAAGT TTATGATGAT 
GTATTGAATA CTTCTGAGGG AGAAAGTAAA AGTAACAAAG ATGCAGACCA TTCCTTTTCA 
AATCCTTCTG TACCAGAATC AAATCTAAGG ACTAGAAATG CCAGTAAGAG GTTACATAAG 
CGAGATTCTA TAGAAAATAG CATGGGGGAA TCCTCAAAAA TAGGGACATC AGACATTTCT 
TTGCTTTCTG AAAAAACTCT TCAGACAGTA GAATGCCAGC ACAAGAGAAG TAGGAGGGTG 
AGGCGATCTA AAGGTTGTGA TTGTTGTGGA GAAAAATCAC AGTCTCAGGA AAAGTCATTT 
ACTGGGTTAA AGGATACAGA AAATTACGAT GTAAAAGTCA GTGAAACAAA AAAGACAGAT 
GGGGAAACAC CTGTATCTAT TTCAGAAACT TCGAAAGCTG ATCTGTCCTC GGAGGTTAAA 
CTTTTAGATG AGCATCATAG TGTGAATTTT CATTTGGATC TCAAGGAAGA GAATGATACT 
ACTAATGATT CATTAATTAT ATCTGAAACT GAGTTAAAAG AAAATACTAC TCAAAGCTTT 
CTTCCTTCTG AAATGGTAAA ATTTAAAGAA ACTTCTCGTA GGGATTCTGA GGAAGCAATA 
TCTCTAGAAA CCCAGGAGCC ATCTAATAAG AAATGTAAAA CTGTTGACCC ACATTTAGAT 
GTTTCCAAAG ATATTTCACT GAAATGTTTT TCTTTGGAGA CTAAAGAAGA AAAGTCAGAG 
GCCACTTCAA AAGAGAAATT GAGTACAGAG AACATTGTTA TTGTTGAATC AAATGCAGAA 
TCAAATCCAG AAGTAGATGC TATGGAATTG AATGTAGAAA ATGATCAGTC TGAAGCTGGC 
TTCTCTGAAC AAAAGAATGT CAAAACTGGT ATTCCAGGGA AAGAAGTAAC AGAGGAAAAC 
AGTGAAAGAA TGGATACACC TGAAAAACTG AAAGCCAATG AAGCAGTAAC TGAGGAATTT 
AATTCAGCTA CTGATTATTC GGATACTCCG ACATCTGTAA AAGTGAATGC TCAGGTAGAG 
ATCTCTGAAC AAATAGCGGT TGGGGAGCTA GGTGGAGGAA GTGATGAATC TGATCCAGGC 
TCATCTGAAG AAATGAACGC TGAAATGAAA AATTGTGAAA AAACAGCGGT TTGTGAGGTG 
ACTACTGAAT CTGACCACGT CACTGAAACC GAAGACGAAG ATGTAACTAC CACGGCTTCT 
CTGCCTATAG AAGTTGAAAC AAAGGAATTA GATGTAGAAG TCAATAACTT TGTTCCCGAC 
ACACTTGAGA TGATCACTGA AGAAGGAAAG GTTGACCCTA ACAAAACTGA AACGAATACT 
GAACCTAATA AATTGGAGGA AACACAGTTA GATGACAATC AGATGGTAGT GGAAAGTAAT 
GAAACTTTAC CGGAAGTTCA CCATACTTCA GAGAAAGTAG AGGAGATAGC ACAACCTCTG 
ACATCTGAAA CAGATATTTC TGGACTAATA TCAGAAGACA GTAATACATC TCCTCAAAAA 
TCGAAGGATA TTGATCCTTT ACTCATGTCA GCAAACGAGA GTCCTAGTGG CATGCACACA 
CGTTGTGTGT GGTCTCCTTT GGCTTCTCCA TCCACCAGCA TTTTAAAGAG AGGACTAAAA 
AGACCCCAAG AAGATGAAAT CTCGTCACCT GTCCACAAGG TTCGCCGTGT CTCCTTTGCA 
GATCCTATAT ACCAAGCAGG ATTGGCAGAT GACATTGATA GGCGATGCTC TATTGTTAGG 
TGCCATTCTT CAAATAGTTC TCTCATGGTA AAAAGTGTTA AAACTTCACC TACTGTGCAA 
TCTAAGATTG CAGAAATGGC TAAGGAATCT GTGCCATGCC CAACAGAAAG TGTTTACCCT 
GCTTTGGTGA ACTGTGTAGC ACCAGTTGAA ATCATTTTAC CTCAGATTAC ATCCAACATG 
TGGGCAAGAG GACTGGGACA GCTCATTAGA GCCAAGAATA TAAAAACAAT TGGCGATTTG 
AGTACCCTTA CAGCATCTGA AATAAAAACT CTTCCTATCC GTTCTCCAAA AGTGTCTAAC 
GTTAAAAAGG CTCTCAGAAT ATATCATGAG CAGCAGGTGA AGTCTCGTGG ACTAGAAGAG 
ATTCCAGTTT TTGATATTTC TGAAAAAACA ATAAATGGAA TGGAAAATAA ATCTATCTCT 
CCTGATGAAG AAAGACTTGC CTCAGATTTA ATTGATCCTG TTGCTTTAGA AGCTCCGTTA 
TCTAAAAATC TTGTGGCACA GATTAGCGCA CTTGCTCTTC AACTAGATTC AGAAGATCTC 
TATAATTATT CAGGAAGCCA GCTATTTGAA ATGCACGAGA AACTTGGTAG CATGGCAAAC 
TCTATAATTA AAAATCTGCA GTCACGTTGG AGGTCACCAG CCCATGAAAA TTCTTTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RIF1 ( XM_010826490.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010824792.1 Bos taurus replication timing regulatory factor 1 (RIF1), transcript variant X3, mRNA.

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CloneID	OBa55066
Clone ID Related Accession (Same CDS sequence)	XM_010802074.3 , XM_010802074.2 , XM_010826490.2
Accession Version	XM_010826490.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7320bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	telomere-associated protein RIF1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644591.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010826490.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGCCG CGGATCAGAG CCCCCTGGCA CCGCTGTTGG AGACTTTGGA AGACCCTTCC 
GCCTCCCTTG GAGAGCAGAC CGACGCTTAC CTGACCCTGA CCAGTCGTAT GACTGGAGAA 
GATGGAAAAG AAATCATTAC AGAAATTGAG AAAAAACTTC CTCAGCTATT TAAAGTTTTA 
AAGACTCACA TTGCCAGTCA AAACTCAGAG CTCAGTAGTG CTGCTCTACA GGCCTTGGGG 
TTTTGCTTAT ATAATCCCAA AATTGCCTCT GGATTATCAG AGATAAATAT TCAAGAACTG 
CTTTCAAAAC TGAATGATAT TGTTAAAAGT TCAGATAAAA ATGTACGTAC TAGGGCACTT 
TGGGTGATAT CCAAGCAGAC GTTTCCCACT GAAGTCGTTG GCAAAGTAGT TTCTAGTATC 
ATTGACTCAC TAGAAATAGT ATTTAATAGA GGAGAGATGC TTTCTGCTGT TGTGGATTAT 
GAAGCATTAA ATGTCATTAT AAGACTAATT GAACAAGCCC CAGTTCAAAT GGGAGAAGAG 
TCAATTAGGT GGGCAAAATT GGTCATACCT TTACTGGTTC ATTCAGCCCA AAAAGTACAT 
TTGCGGGGAG CCACTGCTCT AGAGATGGGA ATGCCTTTGT TGCTTCAGAA ACAGCAAGAA 
ATAGCATCTA TCACAGAGCA GCTTATGACT ACCAAATTAC TTTCAGAACT CCAGAAACTG 
TTTATGAGTA AAAATGAGAC TTATGTGTTA AAATTGTGGC CTTTATTTGT CAAACTTCTT 
GGGAAGACCT TGCATCGAAG TGGGAGTTTC ATTAATTCTC TGTTACAGCT GGAAGAACTG 
GGATTTCGTA GTGGAGCACC CATGATTAAA AAAATAGCTT TTATTGCTTG GAAGAGTTTA 
ATAGATAATT TTGCTTTAAA TCCAGAAATA CTGTGTAGTG CAAAAAGACT GAAGTTGTTG 
ATGCAGCCTT TGAGTTCTAT CCATGTGAGA ACAGAAACTC TAGCGCTGAC AAAACTAGAA 
GTCTGGTGGT ATTTGTTGAT GAGACTAGGA CCTCATCTTC CTGCTAATTT TGAACAGGTT 
TGTGTGCCTC TGATTCAGAG CACAATAAGC ATTGATTCTA ATGCTTCACC TCAAGGGAGT 
TCATCTCGTG TAGCTACTAC TCCAGGTTTA AGCCCTATGA CCCCAGTACA CAAAGGTGCT 
TCTCCATATG GAACTCCTGT AACCCCTCGG ATGAACTTGA ATTCAAATTT TGGAATGGCC 
ACAATCCCCT CTATACAACT TTTGGGGCTT GAAATGTTGC TTCATTTTTT GTTGGGTCCA 
GAAGTCGTGA GTTTTGCTAA GCAAAACAAA CTTATACTGA GCCTAGAACC ACTTGAACAT 
CCATTAATCA GCAGCTCTTC TTTTTTCTCC AAACACTCAA ATACACTTAT CACTGCTGTT 
CATGATAGCT TTGTTGCAGT TGGAAAAGAT GCTTCTGATG TAGTTATTAA TGCTATTTGG 
AAGGAGTTAA TTAGCTTGGT GAAGTCAGTT ATTGAATCAG GTAACAAAAA AGAGAGACCA 
GGTTCTGAAG TTTTGACTCT CTTATTAAAA TCTTTGGAAA GCATAGTGAA TTCAGAAGTA 
TTTCCTGTAT TGAAAACACT GGTTCTCATG GAAATTACAA TTAAAGGACT TCCTCAGAAA 
GTATTAGGTT CTCCAGCATA TCAGGTTGCT AATATGGATA TTCTTAATGG AACCCCTGCT 
TTGTTCTTAA TTCAGTTAAC TTTCAATAAT AATCTATTGG AATGTGGTGT GGAAGACGAA 
AGGTTTTTTC TCAACCTGGA AACACTTGTG GGCTGTGTTC TTTCGGGTCC AACTTCACCA 
CTCGCTTTCA GTGACTCTGT CTTAAATGTT GTCAATCAAA ATGCAAAGCA GTTGGATAAC 
AAGGAGCATC TTTGGAGAAT GTGGAGTATT ATAGTCACCC CATTAACAGA ATTGATTAAT 
CAGACCAATG AAGTGAATCA AGGGGATGCC TTAGAACATA ATTTCAGTGC TATCTATGGT 
GCACTGATTT TGCCAATAAA CCATATTTTT CCAGCACAAA AAATTCCAGT GGTCACCATG 
AAGACCTTAC TTAAAACTTG GTCAGAATTA TATAGAGCAT TTGCTCGCTG TGCAGCTTTG 
GTGGCAACTG CGGAAGAGAA TTTATGCTGT GAGGAATTTT GTTCCAAGAT AATGTCCAGT 
TTGGAAGATG AAGGCCTTTC AAATTTATTG TTCTTGGATA GGATCACTCA TATTATTACT 
GTAATGGTTG ATTGCATCGA CTTTTCACCA TATAATATTA AATATCAACC CAAAACTAAA 
TCACCACAGA GACCTACAGA TTGGTCCAAA AAGAAGAAGG AACCTTTAGG GAAGTTGGCT 
TCTTTAATTA AACTTATTGT GAGAGTGATC TATTCTTTTC ACACACTGAG CCTCAAGGAA 
GTACATTCTG ATACTCTCCT CACTATTGGC AACTCAATCA CCAGCATTCT TTCCAATTTA 
CTTGGACATA TTTCTCTGCC TTCTATGATC CAGAAAATAT TTGCAGCTTT AACAAGACCT 
CTGGCATTAT TTTATGAAAA CTCAAAGCTT GATGAAGTTC CTAAAGTGTA TAGTTGTATG 
AACAACAAGT TAGAAAAGCT ACTAGGAGAT ATAATTGCTT GTCTACATGT CAGCTACACT 
GGAACTTACG ACAGTGAACT TCTTGAACAG ATCTCACCAT TATTATGCAT AATATTTTCA 
CACAAGAACA AACAGATAAG AAAACAAAGT GCTCAGTTCT GGAATGCCAC ATTTGCTAAA 
GTGACGATGT TGATTTATCC TGAGGAGTTA AAGCCAATAC TGAAACAAGC CAAACAAAAA 
TCTCTGCTTT TATTGCCTGG TTTGGAAAAT GTTGAAATTA TGGAGGAATC CAGTGGACCA 
TATTCAGATA TGACAGAAAA TTCCCAATTA AATATGAAGA TAAGTGGCAT GGAAAGAAAA 
TCAAGTGGAA AAAGAGATTC TTTTTTGGCA CAAACAAAGG ATACAAAAGA CAGTATGAAA 
CCACCAGCTA AATTGAAACT AGATTCTTCA ACTCCAAAAG CAAAGGGTGA AATGCCTTTG 
GAAGAAGAAA AGTCTACTGA CTTTGTGTTT ATACCTCCAG AAGTAAAAGA AACAAAGGAA 
AGAATACTAA CTGAACACCA GAAAGAAGTA CTCAAAACAA AGAGGTGTGA TATTCCTGCC 
ATGTATAATA ATCTGGATGT TTCACAAGAT ACTTTATTTT CTCAGTATAG TCAGGAAGAA 
TCTATGGAAA TTTCTACTTT AATTGAAAAA TCAAAAGAAG ACTCCAAGAT GATATTTAAG 
GAGGAACAAA AGGAGAATGA CATTGTCGTT CCTCAACATG TCATGGAAAA CTGCGGTATG 
GATGAACATC TTGAAAAGGC TTCCCTTTTG AATAATGAGT GTGGTTCTAT TGAGGAAACC 
AGTCCAAAAA TACTGAGCAG TAATAATGAT GCCAGAAAAA AAGCTTTAAT TGCTTCAAAT 
AAACCAACAG AATGTGCATC TACTACAGAA AATCCATCTG GTGTCAGCAG TAGCTCGGTT 
TCTATTGCCA CCATTTCTGG AGCTCCCTTA CAGCCTACAA GTCGAAGGCA ATCCTTTATT 
ACTTTGGAAA AGTTTGATGG TTCAGAAAAT AGACCTTTTA GTCCATCACC CTTGAATAAT 
ATGTCATCAA CTGTAATAGT GAAAAGTAAC CAGGAAGGCA TGGGTAAAAC AGATAATCCA 
TCAAAAGCAA AGAAGAGAGA AGTGGCTCTT TCCAAATCTG ACTCTGAAAA AATAACACAT 
GGGACTAAGA GATCAAGCCG AAGGTCGAGT AAATCTGAAC CAATAGGGAG TAAAAAGTCT 
AAACCCTTAA TGAGATCTGA ACAAGAGAAA AGTACTCAAG AATCTGTTGA TGGCACAGTA 
GCCTTAGAAA ATAACCCACC TGGCTTGCTT AATCAGACAG AATGTGTGTT AGATAATCAG 
GTTCATCTCT CTGAATCTAC AGTGGAGTTT GAGGATACAA TGCTTGAATC AACAGTGGAG 
AATACTGTAT TAGAAAACAA TACTGTAGAA GAGAGAACCT TAGAAGTTAA CTTGGAATCC 
AAAGAAAACA CACCCCCAGC CATAATAACG GATCAGACAG TAAATGAGGA TAGTCATATT 
CAGGTAACTC CAAATCAGAA AACCCTTAGG CGATCTTTAA GGCGACGTTC AGAAACAGCA 
GAACCAACCA CTGATAGCCA AGATAAAGAA AATAATCATC AAAAAAGGGA AAGACGTAAA 
GAAGAAGAAA AGACTCCGCA GAAGAGTCCG TTACATGTAA AAGATGATGT TTTACCTAAG 
CAAAAATTGA TTTCTGAACA AAGTGTACAG GGAAACTTAG TTGACAAAGG AAATAATTTA 
CATGAGAAGG CTTTTGGGGA GACCAGTGCT AATGCTGAAA TTGATGAAAA TAGAAGAAAG 
CCAGATCTTG AGAATACTAA ATCTGAAGGA GATGGTGTGC CGGACATTGC AGATAAATCC 
TCTGAGAAAC CAGTAAGGGG ACGAACACGA TACCAAACAA GAAGAGCATC CCAGGGTTTA 
CTTTCCAGCA TTGAAAACTC AGAATCTGAT AGTTCTGAGG CAAAAGAAGA AGGTTCTAAA 
AAGAAAAGAT CTGGAAAATG GAAGAACAAA AGCAGTGACA ATGTTGACAT GGAAGATCAA 
GAAGAGAAAA TGGTAAAACA GGAATGTATA AAAATTGAAA ATCAGGCACA TGATTATAAA 
GGGAATCCTG AAGTAGACAA AGATATAAAA TCTCAAATCT GTGAAAAAGG AGATGAAAGT 
ACTACTGTAA GTCTTCAAGA TTCTACCGTA TCTCCAGATT TATTACAAGT TTATGATGAT 
GTATTGAATA CTTCTGAGGG AGAAAGTAAA AGTAACAAAG ATGCAGACCA TTCCTTTTCA 
AATCCTTCTG TACCAGAATC AAATCTAAGG ACTAGAAATG CCAGTAAGAG GTTACATAAG 
CGAGATTCTA TAGAAAATAG CATGGGGGAA TCCTCAAAAA TAGGGACATC AGACATTTCT 
TTGCTTTCTG AAAAAACTCT TCAGACAGTA GAATGCCAGC ACAAGAGAAG TAGGAGGGTG 
AGGCGATCTA AAGGTTGTGA TTGTTGTGGA GAAAAATCAC AGTCTCAGGA AAAGTCATTT 
ACTGGGTTAA AGGATACAGA AAATTACGAT GTAAAAGTCA GTGAAACAAA AAAGACAGAT 
GGGGAAACAC CTGTATCTAT TTCAGAAACT TCGAAAGCTG ATCTGTCCTC GGAGGTTAAA 
CTTTTAGATG AGCATCATAG TGTGAATTTT CATTTGGATC TCAAGGAAGA GAATGATACT 
ACTAATGATT CATTAATTAT ATCTGAAACT GAGTTAAAAG AAAATACTAC TCAAAGCTTT 
CTTCCTTCTG AAATGGTAAA ATTTAAAGAA ACTTCTCGTA GGGATTCTGA GGAAGCAATA 
TCTCTAGAAA CCCAGGAGCC ATCTAATAAG AAATGTAAAA CTGTTGACCC ACATTTAGAT 
GTTTCCAAAG ATATTTCACT GAAATGTTTT TCTTTGGAGA CTAAAGAAGA AAAGTCAGAG 
GCCACTTCAA AAGAGAAATT GAGTACAGAG AACATTGTTA TTGTTGAATC AAATGCAGAA 
TCAAATCCAG AAGTAGATGC TATGGAATTG AATGTAGAAA ATGATCAGTC TGAAGCTGGC 
TTCTCTGAAC AAAAGAATGT CAAAACTGGT ATTCCAGGGA AAGAAGTAAC AGAGGAAAAC 
AGTGAAAGAA TGGATACACC TGAAAAACTG AAAGCCAATG AAGCAGTAAC TGAGGAATTT 
AATTCAGCTA CTGATTATTC GGATACTCCG ACATCTGTAA AAGTGAATGC TCAGGTAGAG 
ATCTCTGAAC AAATAGCGGT TGGGGAGCTA GGTGGAGGAA GTGATGAATC TGATCCAGGC 
TCATCTGAAG AAATGAACGC TGAAATGAAA AATTGTGAAA AAACAGCGGT TTGTGAGGTG 
ACTACTGAAT CTGACCACGT CACTGAAACC GAAGACGAAG ATGTAACTAC CACGGCTTCT 
CTGCCTATAG AAGTTGAAAC AAAGGAATTA GATGTAGAAG TCAATAACTT TGTTCCCGAC 
ACACTTGAGA TGATCACTGA AGAAGGAAAG GTTGACCCTA ACAAAACTGA AACGAATACT 
GAACCTAATA AATTGGAGGA AACACAGTTA GATGACAATC AGATGGTAGT GGAAAGTAAT 
GAAACTTTAC CGGAAGTTCA CCATACTTCA GAGAAAGTAG AGGAGATAGC ACAACCTCTG 
ACATCTGAAA CAGATATTTC TGGACTAATA TCAGAAGACA GTAATACATC TCCTCAAAAA 
TCGAAGGATA TTGATCCTTT ACTCATGTCA GCAAACGAGA GTCCTAGTGG CATGCACACA 
CGTTGTGTGT GGTCTCCTTT GGCTTCTCCA TCCACCAGCA TTTTAAAGAG AGGACTAAAA 
AGACCCCAAG AAGATGAAAT CTCGTCACCT GTCCACAAGG TTCGCCGTGT CTCCTTTGCA 
GATCCTATAT ACCAAGCAGG ATTGGCAGAT GACATTGATA GGCGATGCTC TATTGTTAGG 
TGCCATTCTT CAAATAGTTC TCTCATGGTA AAAAGTGTTA AAACTTCACC TACTGTGCAA 
TCTAAGATTG CAGAAATGGC TAAGGAATCT GTGCCATGCC CAACAGAAAG TGTTTACCCT 
GCTTTGGTGA ACTGTGTAGC ACCAGTTGAA ATCATTTTAC CTCAGATTAC ATCCAACATG 
TGGGCAAGAG GACTGGGACA GCTCATTAGA GCCAAGAATA TAAAAACAAT TGGCGATTTG 
AGTACCCTTA CAGCATCTGA AATAAAAACT CTTCCTATCC GTTCTCCAAA AGTGTCTAAC 
GTTAAAAAGG CTCTCAGAAT ATATCATGAG CAGCAGGTGA AGTCTCGTGG ACTAGAAGAG 
ATTCCAGTTT TTGATATTTC TGAAAAAACA ATAAATGGAA TGGAAAATAA ATCTATCTCT 
CCTGATGAAG AAAGACTTGC CTCAGATTTA ATTGATCCTG TTGCTTTAGA AGCTCCGTTA 
TCTAAAAATC TTGTGGCACA GATTAGCGCA CTTGCTCTTC AACTAGATTC AGAAGATCTC 
TATAATTATT CAGGAAGCCA GCTATTTGAA ATGCACGAGA AACTTGGTAG CATGGCAAAC 
TCTATAATTA AAAATCTGCA GTCACGTTGG AGGTCACCAG CCCATGAAAA TTCTTTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010824792.1
CDS	146..7465
Translation	MTAADQSPLAPLLETLEDPSASLGEQTDAYLTLTSRMTGEDGKEIITEIEKKLPQLFKVLKTHIASQNSELSSAALQALGFCLYNPKIASGLSEINIQELLSKLNDIVKSSDKNVRTRALWVISKQTFPTEVVGKVVSSIIDSLEIVFNRGEMLSAVVDYEALNVIIRLIEQAPVQMGEESIRWAKLVIPLLVHSAQKVHLRGATALEMGMPLLLQKQQEIASITEQLMTTKLLSELQKLFMSKNETYVLKLWPLFVKLLGKTLHRSGSFINSLLQLEELGFRSGAPMIKKIAFIAWKSLIDNFALNPEILCSAKRLKLLMQPLSSIHVRTETLALTKLEVWWYLLMRLGPHLPANFEQVCVPLIQSTISIDSNASPQGSSSRVATTPGLSPMTPVHKGASPYGTPVTPRMNLNSNFGMATIPSIQLLGLEMLLHFLLGPEVVSFAKQNKLILSLEPLEHPLISSSSFFSKHSNTLITAVHDSFVAVGKDASDVVINAIWKELISLVKSVIESGNKKERPGSEVLTLLLKSLESIVNSEVFPVLKTLVLMEITIKGLPQKVLGSPAYQVANMDILNGTPALFLIQLTFNNNLLECGVEDERFFLNLETLVGCVLSGPTSPLAFSDSVLNVVNQNAKQLDNKEHLWRMWSIIVTPLTELINQTNEVNQGDALEHNFSAIYGALILPINHIFPAQKIPVVTMKTLLKTWSELYRAFARCAALVATAEENLCCEEFCSKIMSSLEDEGLSNLLFLDRITHIITVMVDCIDFSPYNIKYQPKTKSPQRPTDWSKKKKEPLGKLASLIKLIVRVIYSFHTLSLKEVHSDTLLTIGNSITSILSNLLGHISLPSMIQKIFAALTRPLALFYENSKLDEVPKVYSCMNNKLEKLLGDIIACLHVSYTGTYDSELLEQISPLLCIIFSHKNKQIRKQSAQFWNATFAKVTMLIYPEELKPILKQAKQKSLLLLPGLENVEIMEESSGPYSDMTENSQLNMKISGMERKSSGKRDSFLAQTKDTKDSMKPPAKLKLDSSTPKAKGEMPLEEEKSTDFVFIPPEVKETKERILTEHQKEVLKTKRCDIPAMYNNLDVSQDTLFSQYSQEESMEISTLIEKSKEDSKMIFKEEQKENDIVVPQHVMENCGMDEHLEKASLLNNECGSIEETSPKILSSNNDARKKALIASNKPTECASTTENPSGVSSSSVSIATISGAPLQPTSRRQSFITLEKFDGSENRPFSPSPLNNMSSTVIVKSNQEGMGKTDNPSKAKKREVALSKSDSEKITHGTKRSSRRSSKSEPIGSKKSKPLMRSEQEKSTQESVDGTVALENNPPGLLNQTECVLDNQVHLSESTVEFEDTMLESTVENTVLENNTVEERTLEVNLESKENTPPAIITDQTVNEDSHIQVTPNQKTLRRSLRRRSETAEPTTDSQDKENNHQKRERRKEEEKTPQKSPLHVKDDVLPKQKLISEQSVQGNLVDKGNNLHEKAFGETSANAEIDENRRKPDLENTKSEGDGVPDIADKSSEKPVRGRTRYQTRRASQGLLSSIENSESDSSEAKEEGSKKKRSGKWKNKSSDNVDMEDQEEKMVKQECIKIENQAHDYKGNPEVDKDIKSQICEKGDESTTVSLQDSTVSPDLLQVYDDVLNTSEGESKSNKDADHSFSNPSVPESNLRTRNASKRLHKRDSIENSMGESSKIGTSDISLLSEKTLQTVECQHKRSRRVRRSKGCDCCGEKSQSQEKSFTGLKDTENYDVKVSETKKTDGETPVSISETSKADLSSEVKLLDEHHSVNFHLDLKEENDTTNDSLIISETELKENTTQSFLPSEMVKFKETSRRDSEEAISLETQEPSNKKCKTVDPHLDVSKDISLKCFSLETKEEKSEATSKEKLSTENIVIVESNAESNPEVDAMELNVENDQSEAGFSEQKNVKTGIPGKEVTEENSERMDTPEKLKANEAVTEEFNSATDYSDTPTSVKVNAQVEISEQIAVGELGGGSDESDPGSSEEMNAEMKNCEKTAVCEVTTESDHVTETEDEDVTTTASLPIEVETKELDVEVNNFVPDTLEMITEEGKVDPNKTETNTEPNKLEETQLDDNQMVVESNETLPEVHHTSEKVEEIAQPLTSETDISGLISEDSNTSPQKSKDIDPLLMSANESPSGMHTRCVWSPLASPSTSILKRGLKRPQEDEISSPVHKVRRVSFADPIYQAGLADDIDRRCSIVRCHSSNSSLMVKSVKTSPTVQSKIAEMAKESVPCPTESVYPALVNCVAPVEIILPQITSNMWARGLGQLIRAKNIKTIGDLSTLTASEIKTLPIRSPKVSNVKKALRIYHEQQVKSRGLEEIPVFDISEKTINGMENKSISPDEERLASDLIDPVALEAPLSKNLVAQISALALQLDSEDLYNYSGSQLFEMHEKLGSMANSIIKNLQSRWRSPAHENSF

Target ORF information:

RefSeq Version	XM_010826490.2
Organism	Bos taurus(cattle)
Definition	Bos taurus replication timing regulatory factor 1 (RIF1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010826490.2

ORF Insert Sequence:

ATGACGGCCG CGGATCAGAG CCCCCTGGCA CCGCTGTTGG AGACTTTGGA AGACCCTTCC 
GCCTCCCTTG GAGAGCAGAC CGACGCTTAC CTGACCCTGA CCAGTCGTAT GACTGGAGAA 
GATGGAAAAG AAATCATTAC AGAAATTGAG AAAAAACTTC CTCAGCTATT TAAAGTTTTA 
AAGACTCACA TTGCCAGTCA AAACTCAGAG CTCAGTAGTG CTGCTCTACA GGCCTTGGGG 
TTTTGCTTAT ATAATCCCAA AATTGCCTCT GGATTATCAG AGATAAATAT TCAAGAACTG 
CTTTCAAAAC TGAATGATAT TGTTAAAAGT TCAGATAAAA ATGTACGTAC TAGGGCACTT 
TGGGTGATAT CCAAGCAGAC GTTTCCCACT GAAGTCGTTG GCAAAGTAGT TTCTAGTATC 
ATTGACTCAC TAGAAATAGT ATTTAATAGA GGAGAGATGC TTTCTGCTGT TGTGGATTAT 
GAAGCATTAA ATGTCATTAT AAGACTAATT GAACAAGCCC CAGTTCAAAT GGGAGAAGAG 
TCAATTAGGT GGGCAAAATT GGTCATACCT TTACTGGTTC ATTCAGCCCA AAAAGTACAT 
TTGCGGGGAG CCACTGCTCT AGAGATGGGA ATGCCTTTGT TGCTTCAGAA ACAGCAAGAA 
ATAGCATCTA TCACAGAGCA GCTTATGACT ACCAAATTAC TTTCAGAACT CCAGAAACTG 
TTTATGAGTA AAAATGAGAC TTATGTGTTA AAATTGTGGC CTTTATTTGT CAAACTTCTT 
GGGAAGACCT TGCATCGAAG TGGGAGTTTC ATTAATTCTC TGTTACAGCT GGAAGAACTG 
GGATTTCGTA GTGGAGCACC CATGATTAAA AAAATAGCTT TTATTGCTTG GAAGAGTTTA 
ATAGATAATT TTGCTTTAAA TCCAGAAATA CTGTGTAGTG CAAAAAGACT GAAGTTGTTG 
ATGCAGCCTT TGAGTTCTAT CCATGTGAGA ACAGAAACTC TAGCGCTGAC AAAACTAGAA 
GTCTGGTGGT ATTTGTTGAT GAGACTAGGA CCTCATCTTC CTGCTAATTT TGAACAGGTT 
TGTGTGCCTC TGATTCAGAG CACAATAAGC ATTGATTCTA ATGCTTCACC TCAAGGGAGT 
TCATCTCGTG TAGCTACTAC TCCAGGTTTA AGCCCTATGA CCCCAGTACA CAAAGGTGCT 
TCTCCATATG GAACTCCTGT AACCCCTCGG ATGAACTTGA ATTCAAATTT TGGAATGGCC 
ACAATCCCCT CTATACAACT TTTGGGGCTT GAAATGTTGC TTCATTTTTT GTTGGGTCCA 
GAAGTCGTGA GTTTTGCTAA GCAAAACAAA CTTATACTGA GCCTAGAACC ACTTGAACAT 
CCATTAATCA GCAGCTCTTC TTTTTTCTCC AAACACTCAA ATACACTTAT CACTGCTGTT 
CATGATAGCT TTGTTGCAGT TGGAAAAGAT GCTTCTGATG TAGTTATTAA TGCTATTTGG 
AAGGAGTTAA TTAGCTTGGT GAAGTCAGTT ATTGAATCAG GTAACAAAAA AGAGAGACCA 
GGTTCTGAAG TTTTGACTCT CTTATTAAAA TCTTTGGAAA GCATAGTGAA TTCAGAAGTA 
TTTCCTGTAT TGAAAACACT GGTTCTCATG GAAATTACAA TTAAAGGACT TCCTCAGAAA 
GTATTAGGTT CTCCAGCATA TCAGGTTGCT AATATGGATA TTCTTAATGG AACCCCTGCT 
TTGTTCTTAA TTCAGTTAAC TTTCAATAAT AATCTATTGG AATGTGGTGT GGAAGACGAA 
AGGTTTTTTC TCAACCTGGA AACACTTGTG GGCTGTGTTC TTTCGGGTCC AACTTCACCA 
CTCGCTTTCA GTGACTCTGT CTTAAATGTT GTCAATCAAA ATGCAAAGCA GTTGGATAAC 
AAGGAGCATC TTTGGAGAAT GTGGAGTATT ATAGTCACCC CATTAACAGA ATTGATTAAT 
CAGACCAATG AAGTGAATCA AGGGGATGCC TTAGAACATA ATTTCAGTGC TATCTATGGT 
GCACTGATTT TGCCAATAAA CCATATTTTT CCAGCACAAA AAATTCCAGT GGTCACCATG 
AAGACCTTAC TTAAAACTTG GTCAGAATTA TATAGAGCAT TTGCTCGCTG TGCAGCTTTG 
GTGGCAACTG CGGAAGAGAA TTTATGCTGT GAGGAATTTT GTTCCAAGAT AATGTCCAGT 
TTGGAAGATG AAGGCCTTTC AAATTTATTG TTCTTGGATA GGATCACTCA TATTATTACT 
GTAATGGTTG ATTGCATCGA CTTTTCACCA TATAATATTA AATATCAACC CAAAACTAAA 
TCACCACAGA GACCTACAGA TTGGTCCAAA AAGAAGAAGG AACCTTTAGG GAAGTTGGCT 
TCTTTAATTA AACTTATTGT GAGAGTGATC TATTCTTTTC ACACACTGAG CCTCAAGGAA 
GTACATTCTG ATACTCTCCT CACTATTGGC AACTCAATCA CCAGCATTCT TTCCAATTTA 
CTTGGACATA TTTCTCTGCC TTCTATGATC CAGAAAATAT TTGCAGCTTT AACAAGACCT 
CTGGCATTAT TTTATGAAAA CTCAAAGCTT GATGAAGTTC CTAAAGTGTA TAGTTGTATG 
AACAACAAGT TAGAAAAGCT ACTAGGAGAT ATAATTGCTT GTCTACATGT CAGCTACACT 
GGAACTTACG ACAGTGAACT TCTTGAACAG ATCTCACCAT TATTATGCAT AATATTTTCA 
CACAAGAACA AACAGATAAG AAAACAAAGT GCTCAGTTCT GGAATGCCAC ATTTGCTAAA 
GTGACGATGT TGATTTATCC TGAGGAGTTA AAGCCAATAC TGAAACAAGC CAAACAAAAA 
TCTCTGCTTT TATTGCCTGG TTTGGAAAAT GTTGAAATTA TGGAGGAATC CAGTGGACCA 
TATTCAGATA TGACAGAAAA TTCCCAATTA AATATGAAGA TAAGTGGCAT GGAAAGAAAA 
TCAAGTGGAA AAAGAGATTC TTTTTTGGCA CAAACAAAGG ATACAAAAGA CAGTATGAAA 
CCACCAGCTA AATTGAAACT AGATTCTTCA ACTCCAAAAG CAAAGGGTGA AATGCCTTTG 
GAAGAAGAAA AGTCTACTGA CTTTGTGTTT ATACCTCCAG AAGTAAAAGA AACAAAGGAA 
AGAATACTAA CTGAACACCA GAAAGAAGTA CTCAAAACAA AGAGGTGTGA TATTCCTGCC 
ATGTATAATA ATCTGGATGT TTCACAAGAT ACTTTATTTT CTCAGTATAG TCAGGAAGAA 
TCTATGGAAA TTTCTACTTT AATTGAAAAA TCAAAAGAAG ACTCCAAGAT GATATTTAAG 
GAGGAACAAA AGGAGAATGA CATTGTCGTT CCTCAACATG TCATGGAAAA CTGCGGTATG 
GATGAACATC TTGAAAAGGC TTCCCTTTTG AATAATGAGT GTGGTTCTAT TGAGGAAACC 
AGTCCAAAAA TACTGAGCAG TAATAATGAT GCCAGAAAAA AAGCTTTAAT TGCTTCAAAT 
AAACCAACAG AATGTGCATC TACTACAGAA AATCCATCTG GTGTCAGCAG TAGCTCGGTT 
TCTATTGCCA CCATTTCTGG AGCTCCCTTA CAGCCTACAA GTCGAAGGCA ATCCTTTATT 
ACTTTGGAAA AGTTTGATGG TTCAGAAAAT AGACCTTTTA GTCCATCACC CTTGAATAAT 
ATGTCATCAA CTGTAATAGT GAAAAGTAAC CAGGAAGGCA TGGGTAAAAC AGATAATCCA 
TCAAAAGCAA AGAAGAGAGA AGTGGCTCTT TCCAAATCTG ACTCTGAAAA AATAACACAT 
GGGACTAAGA GATCAAGCCG AAGGTCGAGT AAATCTGAAC CAATAGGGAG TAAAAAGTCT 
AAACCCTTAA TGAGATCTGA ACAAGAGAAA AGTACTCAAG AATCTGTTGA TGGCACAGTA 
GCCTTAGAAA ATAACCCACC TGGCTTGCTT AATCAGACAG AATGTGTGTT AGATAATCAG 
GTTCATCTCT CTGAATCTAC AGTGGAGTTT GAGGATACAA TGCTTGAATC AACAGTGGAG 
AATACTGTAT TAGAAAACAA TACTGTAGAA GAGAGAACCT TAGAAGTTAA CTTGGAATCC 
AAAGAAAACA CACCCCCAGC CATAATAACG GATCAGACAG TAAATGAGGA TAGTCATATT 
CAGGTAACTC CAAATCAGAA AACCCTTAGG CGATCTTTAA GGCGACGTTC AGAAACAGCA 
GAACCAACCA CTGATAGCCA AGATAAAGAA AATAATCATC AAAAAAGGGA AAGACGTAAA 
GAAGAAGAAA AGACTCCGCA GAAGAGTCCG TTACATGTAA AAGATGATGT TTTACCTAAG 
CAAAAATTGA TTTCTGAACA AAGTGTACAG GGAAACTTAG TTGACAAAGG AAATAATTTA 
CATGAGAAGG CTTTTGGGGA GACCAGTGCT AATGCTGAAA TTGATGAAAA TAGAAGAAAG 
CCAGATCTTG AGAATACTAA ATCTGAAGGA GATGGTGTGC CGGACATTGC AGATAAATCC 
TCTGAGAAAC CAGTAAGGGG ACGAACACGA TACCAAACAA GAAGAGCATC CCAGGGTTTA 
CTTTCCAGCA TTGAAAACTC AGAATCTGAT AGTTCTGAGG CAAAAGAAGA AGGTTCTAAA 
AAGAAAAGAT CTGGAAAATG GAAGAACAAA AGCAGTGACA ATGTTGACAT GGAAGATCAA 
GAAGAGAAAA TGGTAAAACA GGAATGTATA AAAATTGAAA ATCAGGCACA TGATTATAAA 
GGGAATCCTG AAGTAGACAA AGATATAAAA TCTCAAATCT GTGAAAAAGG AGATGAAAGT 
ACTACTGTAA GTCTTCAAGA TTCTACCGTA TCTCCAGATT TATTACAAGT TTATGATGAT 
GTATTGAATA CTTCTGAGGG AGAAAGTAAA AGTAACAAAG ATGCAGACCA TTCCTTTTCA 
AATCCTTCTG TACCAGAATC AAATCTAAGG ACTAGAAATG CCAGTAAGAG GTTACATAAG 
CGAGATTCTA TAGAAAATAG CATGGGGGAA TCCTCAAAAA TAGGGACATC AGACATTTCT 
TTGCTTTCTG AAAAAACTCT TCAGACAGTA GAATGCCAGC ACAAGAGAAG TAGGAGGGTG 
AGGCGATCTA AAGGTTGTGA TTGTTGTGGA GAAAAATCAC AGTCTCAGGA AAAGTCATTT 
ACTGGGTTAA AGGATACAGA AAATTACGAT GTAAAAGTCA GTGAAACAAA AAAGACAGAT 
GGGGAAACAC CTGTATCTAT TTCAGAAACT TCGAAAGCTG ATCTGTCCTC GGAGGTTAAA 
CTTTTAGATG AGCATCATAG TGTGAATTTT CATTTGGATC TCAAGGAAGA GAATGATACT 
ACTAATGATT CATTAATTAT ATCTGAAACT GAGTTAAAAG AAAATACTAC TCAAAGCTTT 
CTTCCTTCTG AAATGGTAAA ATTTAAAGAA ACTTCTCGTA GGGATTCTGA GGAAGCAATA 
TCTCTAGAAA CCCAGGAGCC ATCTAATAAG AAATGTAAAA CTGTTGACCC ACATTTAGAT 
GTTTCCAAAG ATATTTCACT GAAATGTTTT TCTTTGGAGA CTAAAGAAGA AAAGTCAGAG 
GCCACTTCAA AAGAGAAATT GAGTACAGAG AACATTGTTA TTGTTGAATC AAATGCAGAA 
TCAAATCCAG AAGTAGATGC TATGGAATTG AATGTAGAAA ATGATCAGTC TGAAGCTGGC 
TTCTCTGAAC AAAAGAATGT CAAAACTGGT ATTCCAGGGA AAGAAGTAAC AGAGGAAAAC 
AGTGAAAGAA TGGATACACC TGAAAAACTG AAAGCCAATG AAGCAGTAAC TGAGGAATTT 
AATTCAGCTA CTGATTATTC GGATACTCCG ACATCTGTAA AAGTGAATGC TCAGGTAGAG 
ATCTCTGAAC AAATAGCGGT TGGGGAGCTA GGTGGAGGAA GTGATGAATC TGATCCAGGC 
TCATCTGAAG AAATGAACGC TGAAATGAAA AATTGTGAAA AAACAGCGGT TTGTGAGGTG 
ACTACTGAAT CTGACCACGT CACTGAAACC GAAGACGAAG ATGTAACTAC CACGGCTTCT 
CTGCCTATAG AAGTTGAAAC AAAGGAATTA GATGTAGAAG TCAATAACTT TGTTCCCGAC 
ACACTTGAGA TGATCACTGA AGAAGGAAAG GTTGACCCTA ACAAAACTGA AACGAATACT 
GAACCTAATA AATTGGAGGA AACACAGTTA GATGACAATC AGATGGTAGT GGAAAGTAAT 
GAAACTTTAC CGGAAGTTCA CCATACTTCA GAGAAAGTAG AGGAGATAGC ACAACCTCTG 
ACATCTGAAA CAGATATTTC TGGACTAATA TCAGAAGACA GTAATACATC TCCTCAAAAA 
TCGAAGGATA TTGATCCTTT ACTCATGTCA GCAAACGAGA GTCCTAGTGG CATGCACACA 
CGTTGTGTGT GGTCTCCTTT GGCTTCTCCA TCCACCAGCA TTTTAAAGAG AGGACTAAAA 
AGACCCCAAG AAGATGAAAT CTCGTCACCT GTCCACAAGG TTCGCCGTGT CTCCTTTGCA 
GATCCTATAT ACCAAGCAGG ATTGGCAGAT GACATTGATA GGCGATGCTC TATTGTTAGG 
TGCCATTCTT CAAATAGTTC TCTCATGGTA AAAAGTGTTA AAACTTCACC TACTGTGCAA 
TCTAAGATTG CAGAAATGGC TAAGGAATCT GTGCCATGCC CAACAGAAAG TGTTTACCCT 
GCTTTGGTGA ACTGTGTAGC ACCAGTTGAA ATCATTTTAC CTCAGATTAC ATCCAACATG 
TGGGCAAGAG GACTGGGACA GCTCATTAGA GCCAAGAATA TAAAAACAAT TGGCGATTTG 
AGTACCCTTA CAGCATCTGA AATAAAAACT CTTCCTATCC GTTCTCCAAA AGTGTCTAAC 
GTTAAAAAGG CTCTCAGAAT ATATCATGAG CAGCAGGTGA AGTCTCGTGG ACTAGAAGAG 
ATTCCAGTTT TTGATATTTC TGAAAAAACA ATAAATGGAA TGGAAAATAA ATCTATCTCT 
CCTGATGAAG AAAGACTTGC CTCAGATTTA ATTGATCCTG TTGCTTTAGA AGCTCCGTTA 
TCTAAAAATC TTGTGGCACA GATTAGCGCA CTTGCTCTTC AACTAGATTC AGAAGATCTC 
TATAATTATT CAGGAAGCCA GCTATTTGAA ATGCACGAGA AACTTGGTAG CATGGCAAAC 
TCTATAATTA AAAATCTGCA GTCACGTTGG AGGTCACCAG CCCATGAAAA TTCTTTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RIF1 ( XM_010802075.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010800377.1 Bos taurus replication timing regulatory factor 1 (RIF1), transcript variant X4, mRNA.

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CloneID	OBa55067
Clone ID Related Accession (Same CDS sequence)	XM_010802075.2 , XM_010802075.3 , XM_010826491.2
Accession Version	XM_010802075.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7251bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	telomere-associated protein RIF1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103838.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010802075.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGCCG CGGATCAGAG CCCCCTGGCA CCGCTGTTGG AGACTTTGGA AGACCCTTCC 
GCCTCCCTTG GAGAGCAGAC CGACGCTTAC CTGACCCTGA CCAGTCGTAT GACTGGAGAA 
GATGGAAAAG AAATCATTAC AGAAATTGAG AAAAAACTTC CTCAGCTATT TAAAGTTTTA 
AAGACTCACA TTGCCAGTCA AAACTCAGAG CTCAGTAGTG CTGCTCTACA GGCCTTGGGG 
TTTTGCTTAT ATAATCCCAA AATTGCCTCT GGATTATCAG AGATAAATAT TCAAGAACTG 
CTTTCAAAAC TGAATGATAT TGTTAAAAGT TCAGATAAAA ATGTACGTAC TAGGGCACTT 
TGGGTGATAT CCAAGCAGAC GTTTCCCACT GAAGTCGTTG GCAAAGTAGT TTCTAGTATC 
ATTGACTCAC TAGAAATAGT ATTTAATAGA GGAGAGATGC TTTCTGCTGT TGTGGATTAT 
GAAGCATTAA ATGTCATTAT AAGACTAATT GAACAAGCCC CAGTTCAAAT GGGAGAAGAG 
TCAATTAGGT GGGCAAAATT GGTCATACCT TTACTGGTTC ATTCAGCCCA AAAAGTACAT 
TTGCGGGGAG CCACTGCTCT AGAGATGGGA ATGCCTTTGT TGCTTCAGAA ACAGCAAGAA 
ATAGCATCTA TCACAGAGCA GCTTATGACT ACCAAATTAC TTTCAGAACT CCAGAAACTG 
TTTATGAGTA AAAATGAGAC TTATGTGTTA AAATTGTGGC CTTTATTTGT CAAACTTCTT 
GGGAAGACCT TGCATCGAAG TGGGAGTTTC ATTAATTCTC TGTTACAGCT GGAAGAACTG 
GGATTTCGTA GTGGAGCACC CATGATTAAA AAAATAGCTT TTATTGCTTG GAAGAGTTTA 
ATAGATAATT TTGCTTTAAA TCCAGAAATA CTGTGTAGTG CAAAAAGACT GAAGTTGTTG 
ATGCAGCCTT TGAGTTCTAT CCATGTGAGA ACAGAAACTC TAGCGCTGAC AAAACTAGAA 
GTCTGGTGGT ATTTGTTGAT GAGACTAGGA CCTCATCTTC CTGCTAATTT TGAACAGGTT 
TGTGTGCCTC TGATTCAGAG CACAATAAGC ATTGATTCTA ATGCTTCACC TCAAGGGAGT 
TCATCTCGTG TAGCTACTAC TCCAGGTTTA AGCCCTATGA CCCCAGTACA CAAAGGTGCT 
TCTCCATATG GAACTCCTGT AACCCCTCGG ATGAACTTGA ATTCAAATTT TGGAATGGCC 
ACAATCCCCT CTATACAACT TTTGGGGCTT GAAATGTTGC TTCATTTTTT GTTGGGTCCA 
GAAGTCGTGA GTTTTGCTAA GCAAAACAAA CTTATACTGA GCCTAGAACC ACTTGAACAT 
CCATTAATCA GCAGCTCTTC TTTTTTCTCC AAACACTCAA ATACACTTAT CACTGCTGTT 
CATGATAGCT TTGTTGCAGT TGGAAAAGAT GCTTCTGATG TAGTTATTAA TGCTATTTGG 
AAGGAGTTAA TTAGCTTGGT GAAGTCAGTT ATTGAATCAG GTAACAAAAA AGAGAGACCA 
GGTTCTGAAG TTTTGACTCT CTTATTAAAA TCTTTGGAAA GCATAGTGAA TTCAGAAGTA 
TTTCCTGTAT TGAAAACACT GGTTCTCATG GAAATTACAA TTAAAGGACT TCCTCAGAAA 
GTATTAGGTT CTCCAGCATA TCAGGTTGCT AATATGGATA TTCTTAATGG AACCCCTGCT 
TTGTTCTTAA TTCAGTTAAC TTTCAATAAT AATCTATTGG AATGTGGTGT GGAAGACGAA 
AGGTTTTTTC TCAACCTGGA AACACTTGTG GGCTGTGTTC TTTCGGGTCC AACTTCACCA 
CTCGCTTTCA GTGACTCTGT CTTAAATGTT GTCAATCAAA ATGCAAAGCA GTTGGATAAC 
AAGGAGCATC TTTGGAGAAT GTGGAGTATT ATAGTCACCC CATTAACAGA ATTGATTAAT 
CAGACCAATG AAGTGAATCA AGGGGATGCC TTAGAACATA ATTTCAGTGC TATCTATGGT 
GCACTGATTT TGCCAATAAA CCATATTTTT CCAGCACAAA AAATTCCAGT GGTCACCATG 
AAGACCTTAC TTAAAACTTG GTCAGAATTA TATAGAGCAT TTGCTCGCTG TGCAGCTTTG 
GTGGCAACTG CGGAAGAGAA TTTATGCTGT GAGGAATTTT GTTCCAAGAT AATGTCCAGT 
TTGGAAGATG AAGGCCTTTC AAATTTATTG TTCTTGGATA GGATCACTCA TATTATTACT 
GTAATGGTTG ATTGCATCGA CTTTTCACCA TATAATATTA AATATCAACC CAAAACTAAA 
TCACCACAGA GACCTACAGA TTGGTCCAAA AAGAAGAAGG AACCTTTAGG GAAGTTGGCT 
TCTTTAATTA AACTTATTGT GAGAGTGATC TATTCTTTTC ACACACTGAG CCTCAAGGAA 
GTACATTCTG ATACTCTCCT CACTATTGGC AACTCAATCA CCAGCATTCT TTCCAATTTA 
CTTGGACATA TTTCTCTGCC TTCTATGATC CAGAAAATAT TTGCAGCTTT AACAAGACCT 
CTGGCATTAT TTTATGAAAA CTCAAAGCTT GATGAAGTTC CTAAAGTGTA TAGTTGTATG 
AACAACAAGT TAGAAAAGCT ACTAGGAGAT ATAATTGCTT GTCTACATGT CAGCTACACT 
GGAACTTACG ACAGTGAACT TCTTGAACAG ATCTCACCAT TATTATGCAT AATATTTTCA 
CACAAGAACA AACAGATAAG AAAACAAAGT GCTCAGTTCT GGAATGCCAC ATTTGCTAAA 
GTGACGATGT TGATTTATCC TGAGGAGTTA AAGCCAATAC TGAAACAAGC CAAACAAAAA 
TCTCTGCTTT TATTGCCTGG TTTGGAAAAT GTTGAAATTA TGGAGGAATC CAGTGGACCA 
TATTCAGATA TGACAGAAAA TTCCCAATTA AATATGAAGA TAAGTGGCAT GGAAAGAAAA 
TCAAGTGGAA AAAGAGATTC TTTTTTGGCA CAAACAAAGG ATACAAAAGA CAGTATGAAA 
CCACCAGCTA AATTGAAACT AGATTCTTCA ACTCCAAAAG CAAAGGGTGA AATGCCTTTG 
GAAGAAGAAA AGTCTACTGA CTTTGTGTTT ATACCTCCAG AAGTAAAAGA AACAAAGGAA 
AGAATACTAA CTGAACACCA GAAAGAAGTA CTCAAAACAA AGAGGTGTGA TATTCCTGCC 
ATGTATAATA ATCTGGATGT TTCACAAGAT ACTTTATTTT CTCAGTATAG TCAGGAAGAA 
TCTATGGAAA TTTCTACTTT AATTGAAAAA TCAAAAGAAG ACTCCAAGAT GATATTTAAG 
GAGGAACAAA AGGAGAATGA CATTGTCGTT CCTCAACATG TCATGGAAAA CTGCGGTATG 
GATGAACATC TTGAAAAGGC TTCCCTTTTG AATAATGAGT GTGGTTCTAT TGAGGAAACC 
AGTCCAAAAA TACTGAGCAG TAATAATGAT GCCAGAAAAA AAGCTTTAAT TGCTTCAAAT 
AAACCAACAG AATGTGCATC TACTACAGAA AATCCATCTG GTGTCAGCAG TAGCTCGGTT 
TCTATTGCCA CCATTTCTGG AGCTCCCTTA CAGCCTACAA GTCGAAGGCA ATCCTTTATT 
ACTTTGGAAA AGTTTGATGG TTCAGAAAAT AGACCTTTTA GTCCATCACC CTTGAATAAT 
ATGTCATCAA CTGTAATAGT GAAAAGTAAC CAGGAAGGCA TGGGTAAAAC AGATAATCCA 
TCAAAAGCAA AGAAGAGAGA AGTGGCTCTT TCCAAATCTG ACTCTGAAAA AATAACACAT 
GGGACTAAGA GATCAAGCCG AAGGTCGAGT AAATCTGAAC CAATAGGGAG TAAAAAGTCT 
AAACCCTTAA TGAGATCTGA ACAAGAGAAA AGTACTCAAG AATCTGTTGA TGGCACAGTA 
GCCTTAGAAA ATAACCCACC TGGCTTGCTT AATCAGACAG AATGTGTGTT AGATAATCAG 
GTTCATCTCT CTGAATCTAC AGTGGAGTTT GAGGATACAA TGCTTGAATC AACAGTGGAG 
AATACTGTAT TAGAAAACAA TACTGTAGAA GAGAGAACCT TAGAAGTTAA CTTGGAATCC 
AAAGAAAACA CACCCCCAGC CATAATAACG GATCAGACAG TAAATGAGGA TAGTCATATT 
CAGGTAACTC CAAATCAGAA AACCCTTAGG CGATCTTTAA GGCGACGTTC AGAAACAGCA 
GAACCAACCA CTGATAGCCA AGATAAAGAA AATAATCATC AAAAAAGGGA AAGACGTAAA 
GAAGAAGAAA AGACTCCGCA GAAGAGTCCG TTACATGTAA AAGATGATGT TTTACCTAAG 
CAAAAATTGA TTTCTGAACA AAGTGTACAG GGAAACTTAG TTGACAAAGG AAATAATTTA 
CATGAGAAGG CTTTTGGGGA GACCAGTGCT AATGCTGAAA TTGATGAAAA TAGAAGAAAG 
CCAGATCTTG AGAATACTAA ATCTGAAGGA GATGGTGTGC CGGACATTGC AGATAAATCC 
TCTGAGAAAC CAGTAAGGGG ACGAACACGA TACCAAACAA GAAGAGCATC CCAGGGTTTA 
CTTTCCAGCA TTGAAAACTC AGAATCTGAT AGTTCTGAGG CAAAAGAAGA AGGTTCTAAA 
AAGAAAAGAT CTGGAAAATG GAAGAACAAA AGCAGTGACA ATGTTGACAT GGAAGATCAA 
GAAGAGAAAA TGGTAAAACA GGAATGTATA AAAATTGAAA ATCAGGCACA TGATTATAAA 
GGGAATCCTG AAGTAGACAA AGATATAAAA TCTCAAATCT GTGAAAAAGG AGATGAAAGT 
ACTACTGTAA GTCTTCAAGA TTCTACCGTA TCTCCAGATT TATTACAAGT TTATGATGAT 
GTATTGAATA CTTCTGAGGG AGAAAGTAAA AGTAACAAAG ATGCAGACCA TTCCTTTTCA 
AATCCTTCTG TACCAGAATC AAATCTAAGG ACTAGAAATG CCAGTAAGAG GTTACATAAG 
CGAGATTCTA TAGAAAATAG CATGGGGGAA TCCTCAAAAA TAGGGACATC AGACATTTCT 
TTGCTTTCTG AAAAAACTCT TCAGACAGTA GAATGCCAGC ACAAGAGAAG TAGGAGGGTG 
AGGCGATCTA AAGGTTGTGA TTGTTGTGGA GAAAAATCAC AGTCTCAGGA AAAGTCATTT 
ACTGGGTTAA AGGATACAGA AAATTACGAT GTAAAAGTCA GTGAAACAAA AAAGACAGAT 
GGGGAAACAC CTGTATCTAT TTCAGAAACT TCGAAAGCTG ATCTGTCCTC GGAGGTTAAA 
CTTTTAGATG AGCATCATAG TGTGAATTTT CATTTGGATC TCAAGGAAGA GAATGATACT 
ACTAATGATT CATTAATTAT ATCTGAAACT GAGTTAAAAG AAAATACTAC TCAAAGCTTT 
CTTCCTTCTG AAATGGTAAA ATTTAAAGAA ACTTCTCGTA GGGATTCTGA GGAAGCAATA 
TCTCTAGAAA CCCAGGAGCC ATCTAATAAG AAATGTAAAA CTGTTGACCC ACATTTAGAT 
GTTTCCAAAG ATATTTCACT GAAATGTTTT TCTTTGGAGA CTAAAGAAGA AAAGTCAGAG 
GCCACTTCAA AAGAGAAATT GAGTACAGAG AACATTGTTA TTGTTGAATC AAATGCAGAA 
TCAAATCCAG AAGTAGATGC TATGGAATTG AATGTAGAAA ATGATCAGTC TGAAGCTGGC 
TTCTCTGAAC AAAAGAATGT CAAAACTGGT ATTCCAGGGA AAGAAGTAAC AGAGGAAAAC 
AGTGAAAGAA TGGATACACC TGAAAAACTG AAAGCCAATG AAGCAGTAAC TGAGGAATTT 
AATTCAGCTA CTGATTATTC GGATACTCCG ACATCTGTAA AAGTGAATGC TCAGGTAGAG 
ATCTCTGAAC AAATAGCGGT TGGGGAGCTA GGTGGAGGAA GTGATGAATC TGATCCAGGC 
TCATCTGAAG AAATGAACGC TGAAATGAAA AATTGTGAAA AAACAGCGGT TTGTGAGGTG 
ACTACTGAAT CTGACCACGT CACTGAAACC GAAGACGAAG ATGTAACTAC CACGGCTTCT 
CTGCCTATAG AAGTTGAAAC AAAGGAATTA GATGTAGAAG TCAATAACTT TGTTCCCGAC 
ACACTTGAGA TGATCACTGA AGAAGGAAAG GTTGACCCTA ACAAAACTGA AACGAATACT 
GAACCTAATA AATTGGAGGA AACACAGTTA GATGACAATC AGATGGTAGT GGAAAGTAAT 
GAAACTTTAC CGGAAGTTCA CCATACTTCA GAGAAAGTAG AGGAGATAGC ACAACCTCTG 
ACATCTGAAA CAGATATTTC TGGACTAATA TCAGAAGACA GTAATACATC TCCTCAAAAA 
TCGAAGGATA TTGATCCTTT ACTCATGTCA GCAAACGAGA GTCCTAGTGG CATGCACACA 
CGTTGTGTGT GGTCTCCTTT GGCTTCTCCA TCCACCAGCA TTTTAAAGAG AGGACTAAAA 
AGACCCCAAG AAGATGAAAT CTCGTCACCT GTCCACAAGC ATAATACCAC TTCAGCCAAA 
GGATTTCTGT CCCCAGGATC ACGTAGCTCT AAATTTAAGA GCTCAAAGAA GTGTTTAATT 
GCAGAAATGG CTAAGGAATC TGTGCCATGC CCAACAGAAA GTGTTTACCC TGCTTTGGTG 
AACTGTGTAG CACCAGTTGA AATCATTTTA CCTCAGATTA CATCCAACAT GTGGGCAAGA 
GGACTGGGAC AGCTCATTAG AGCCAAGAAT ATAAAAACAA TTGGCGATTT GAGTACCCTT 
ACAGCATCTG AAATAAAAAC TCTTCCTATC CGTTCTCCAA AAGTGTCTAA CGTTAAAAAG 
GCTCTCAGAA TATATCATGA GCAGCAGGTG AAGTCTCGTG GACTAGAAGA GATTCCAGTT 
TTTGATATTT CTGAAAAAAC AATAAATGGA ATGGAAAATA AATCTATCTC TCCTGATGAA 
GAAAGACTTG CCTCAGATTT AATTGATCCT GTTGCTTTAG AAGCTCCGTT ATCTAAAAAT 
CTTGTGGCAC AGATTAGCGC ACTTGCTCTT CAACTAGATT CAGAAGATCT CTATAATTAT 
TCAGGAAGCC AGCTATTTGA AATGCACGAG AAACTTGGTA GCATGGCAAA CTCTATAATT 
AAAAATCTGC AGTCACGTTG GAGGTCACCA GCCCATGAAA ATTCTTTTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010800377.1
CDS	146..7396
Translation	MTAADQSPLAPLLETLEDPSASLGEQTDAYLTLTSRMTGEDGKEIITEIEKKLPQLFKVLKTHIASQNSELSSAALQALGFCLYNPKIASGLSEINIQELLSKLNDIVKSSDKNVRTRALWVISKQTFPTEVVGKVVSSIIDSLEIVFNRGEMLSAVVDYEALNVIIRLIEQAPVQMGEESIRWAKLVIPLLVHSAQKVHLRGATALEMGMPLLLQKQQEIASITEQLMTTKLLSELQKLFMSKNETYVLKLWPLFVKLLGKTLHRSGSFINSLLQLEELGFRSGAPMIKKIAFIAWKSLIDNFALNPEILCSAKRLKLLMQPLSSIHVRTETLALTKLEVWWYLLMRLGPHLPANFEQVCVPLIQSTISIDSNASPQGSSSRVATTPGLSPMTPVHKGASPYGTPVTPRMNLNSNFGMATIPSIQLLGLEMLLHFLLGPEVVSFAKQNKLILSLEPLEHPLISSSSFFSKHSNTLITAVHDSFVAVGKDASDVVINAIWKELISLVKSVIESGNKKERPGSEVLTLLLKSLESIVNSEVFPVLKTLVLMEITIKGLPQKVLGSPAYQVANMDILNGTPALFLIQLTFNNNLLECGVEDERFFLNLETLVGCVLSGPTSPLAFSDSVLNVVNQNAKQLDNKEHLWRMWSIIVTPLTELINQTNEVNQGDALEHNFSAIYGALILPINHIFPAQKIPVVTMKTLLKTWSELYRAFARCAALVATAEENLCCEEFCSKIMSSLEDEGLSNLLFLDRITHIITVMVDCIDFSPYNIKYQPKTKSPQRPTDWSKKKKEPLGKLASLIKLIVRVIYSFHTLSLKEVHSDTLLTIGNSITSILSNLLGHISLPSMIQKIFAALTRPLALFYENSKLDEVPKVYSCMNNKLEKLLGDIIACLHVSYTGTYDSELLEQISPLLCIIFSHKNKQIRKQSAQFWNATFAKVTMLIYPEELKPILKQAKQKSLLLLPGLENVEIMEESSGPYSDMTENSQLNMKISGMERKSSGKRDSFLAQTKDTKDSMKPPAKLKLDSSTPKAKGEMPLEEEKSTDFVFIPPEVKETKERILTEHQKEVLKTKRCDIPAMYNNLDVSQDTLFSQYSQEESMEISTLIEKSKEDSKMIFKEEQKENDIVVPQHVMENCGMDEHLEKASLLNNECGSIEETSPKILSSNNDARKKALIASNKPTECASTTENPSGVSSSSVSIATISGAPLQPTSRRQSFITLEKFDGSENRPFSPSPLNNMSSTVIVKSNQEGMGKTDNPSKAKKREVALSKSDSEKITHGTKRSSRRSSKSEPIGSKKSKPLMRSEQEKSTQESVDGTVALENNPPGLLNQTECVLDNQVHLSESTVEFEDTMLESTVENTVLENNTVEERTLEVNLESKENTPPAIITDQTVNEDSHIQVTPNQKTLRRSLRRRSETAEPTTDSQDKENNHQKRERRKEEEKTPQKSPLHVKDDVLPKQKLISEQSVQGNLVDKGNNLHEKAFGETSANAEIDENRRKPDLENTKSEGDGVPDIADKSSEKPVRGRTRYQTRRASQGLLSSIENSESDSSEAKEEGSKKKRSGKWKNKSSDNVDMEDQEEKMVKQECIKIENQAHDYKGNPEVDKDIKSQICEKGDESTTVSLQDSTVSPDLLQVYDDVLNTSEGESKSNKDADHSFSNPSVPESNLRTRNASKRLHKRDSIENSMGESSKIGTSDISLLSEKTLQTVECQHKRSRRVRRSKGCDCCGEKSQSQEKSFTGLKDTENYDVKVSETKKTDGETPVSISETSKADLSSEVKLLDEHHSVNFHLDLKEENDTTNDSLIISETELKENTTQSFLPSEMVKFKETSRRDSEEAISLETQEPSNKKCKTVDPHLDVSKDISLKCFSLETKEEKSEATSKEKLSTENIVIVESNAESNPEVDAMELNVENDQSEAGFSEQKNVKTGIPGKEVTEENSERMDTPEKLKANEAVTEEFNSATDYSDTPTSVKVNAQVEISEQIAVGELGGGSDESDPGSSEEMNAEMKNCEKTAVCEVTTESDHVTETEDEDVTTTASLPIEVETKELDVEVNNFVPDTLEMITEEGKVDPNKTETNTEPNKLEETQLDDNQMVVESNETLPEVHHTSEKVEEIAQPLTSETDISGLISEDSNTSPQKSKDIDPLLMSANESPSGMHTRCVWSPLASPSTSILKRGLKRPQEDEISSPVHKHNTTSAKGFLSPGSRSSKFKSSKKCLIAEMAKESVPCPTESVYPALVNCVAPVEIILPQITSNMWARGLGQLIRAKNIKTIGDLSTLTASEIKTLPIRSPKVSNVKKALRIYHEQQVKSRGLEEIPVFDISEKTINGMENKSISPDEERLASDLIDPVALEAPLSKNLVAQISALALQLDSEDLYNYSGSQLFEMHEKLGSMANSIIKNLQSRWRSPAHENSF

Target ORF information:

RefSeq Version	XM_010802075.2
Organism	Bos taurus(cattle)
Definition	Bos taurus replication timing regulatory factor 1 (RIF1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010802075.2

ORF Insert Sequence:

ATGACGGCCG CGGATCAGAG CCCCCTGGCA CCGCTGTTGG AGACTTTGGA AGACCCTTCC 
GCCTCCCTTG GAGAGCAGAC CGACGCTTAC CTGACCCTGA CCAGTCGTAT GACTGGAGAA 
GATGGAAAAG AAATCATTAC AGAAATTGAG AAAAAACTTC CTCAGCTATT TAAAGTTTTA 
AAGACTCACA TTGCCAGTCA AAACTCAGAG CTCAGTAGTG CTGCTCTACA GGCCTTGGGG 
TTTTGCTTAT ATAATCCCAA AATTGCCTCT GGATTATCAG AGATAAATAT TCAAGAACTG 
CTTTCAAAAC TGAATGATAT TGTTAAAAGT TCAGATAAAA ATGTACGTAC TAGGGCACTT 
TGGGTGATAT CCAAGCAGAC GTTTCCCACT GAAGTCGTTG GCAAAGTAGT TTCTAGTATC 
ATTGACTCAC TAGAAATAGT ATTTAATAGA GGAGAGATGC TTTCTGCTGT TGTGGATTAT 
GAAGCATTAA ATGTCATTAT AAGACTAATT GAACAAGCCC CAGTTCAAAT GGGAGAAGAG 
TCAATTAGGT GGGCAAAATT GGTCATACCT TTACTGGTTC ATTCAGCCCA AAAAGTACAT 
TTGCGGGGAG CCACTGCTCT AGAGATGGGA ATGCCTTTGT TGCTTCAGAA ACAGCAAGAA 
ATAGCATCTA TCACAGAGCA GCTTATGACT ACCAAATTAC TTTCAGAACT CCAGAAACTG 
TTTATGAGTA AAAATGAGAC TTATGTGTTA AAATTGTGGC CTTTATTTGT CAAACTTCTT 
GGGAAGACCT TGCATCGAAG TGGGAGTTTC ATTAATTCTC TGTTACAGCT GGAAGAACTG 
GGATTTCGTA GTGGAGCACC CATGATTAAA AAAATAGCTT TTATTGCTTG GAAGAGTTTA 
ATAGATAATT TTGCTTTAAA TCCAGAAATA CTGTGTAGTG CAAAAAGACT GAAGTTGTTG 
ATGCAGCCTT TGAGTTCTAT CCATGTGAGA ACAGAAACTC TAGCGCTGAC AAAACTAGAA 
GTCTGGTGGT ATTTGTTGAT GAGACTAGGA CCTCATCTTC CTGCTAATTT TGAACAGGTT 
TGTGTGCCTC TGATTCAGAG CACAATAAGC ATTGATTCTA ATGCTTCACC TCAAGGGAGT 
TCATCTCGTG TAGCTACTAC TCCAGGTTTA AGCCCTATGA CCCCAGTACA CAAAGGTGCT 
TCTCCATATG GAACTCCTGT AACCCCTCGG ATGAACTTGA ATTCAAATTT TGGAATGGCC 
ACAATCCCCT CTATACAACT TTTGGGGCTT GAAATGTTGC TTCATTTTTT GTTGGGTCCA 
GAAGTCGTGA GTTTTGCTAA GCAAAACAAA CTTATACTGA GCCTAGAACC ACTTGAACAT 
CCATTAATCA GCAGCTCTTC TTTTTTCTCC AAACACTCAA ATACACTTAT CACTGCTGTT 
CATGATAGCT TTGTTGCAGT TGGAAAAGAT GCTTCTGATG TAGTTATTAA TGCTATTTGG 
AAGGAGTTAA TTAGCTTGGT GAAGTCAGTT ATTGAATCAG GTAACAAAAA AGAGAGACCA 
GGTTCTGAAG TTTTGACTCT CTTATTAAAA TCTTTGGAAA GCATAGTGAA TTCAGAAGTA 
TTTCCTGTAT TGAAAACACT GGTTCTCATG GAAATTACAA TTAAAGGACT TCCTCAGAAA 
GTATTAGGTT CTCCAGCATA TCAGGTTGCT AATATGGATA TTCTTAATGG AACCCCTGCT 
TTGTTCTTAA TTCAGTTAAC TTTCAATAAT AATCTATTGG AATGTGGTGT GGAAGACGAA 
AGGTTTTTTC TCAACCTGGA AACACTTGTG GGCTGTGTTC TTTCGGGTCC AACTTCACCA 
CTCGCTTTCA GTGACTCTGT CTTAAATGTT GTCAATCAAA ATGCAAAGCA GTTGGATAAC 
AAGGAGCATC TTTGGAGAAT GTGGAGTATT ATAGTCACCC CATTAACAGA ATTGATTAAT 
CAGACCAATG AAGTGAATCA AGGGGATGCC TTAGAACATA ATTTCAGTGC TATCTATGGT 
GCACTGATTT TGCCAATAAA CCATATTTTT CCAGCACAAA AAATTCCAGT GGTCACCATG 
AAGACCTTAC TTAAAACTTG GTCAGAATTA TATAGAGCAT TTGCTCGCTG TGCAGCTTTG 
GTGGCAACTG CGGAAGAGAA TTTATGCTGT GAGGAATTTT GTTCCAAGAT AATGTCCAGT 
TTGGAAGATG AAGGCCTTTC AAATTTATTG TTCTTGGATA GGATCACTCA TATTATTACT 
GTAATGGTTG ATTGCATCGA CTTTTCACCA TATAATATTA AATATCAACC CAAAACTAAA 
TCACCACAGA GACCTACAGA TTGGTCCAAA AAGAAGAAGG AACCTTTAGG GAAGTTGGCT 
TCTTTAATTA AACTTATTGT GAGAGTGATC TATTCTTTTC ACACACTGAG CCTCAAGGAA 
GTACATTCTG ATACTCTCCT CACTATTGGC AACTCAATCA CCAGCATTCT TTCCAATTTA 
CTTGGACATA TTTCTCTGCC TTCTATGATC CAGAAAATAT TTGCAGCTTT AACAAGACCT 
CTGGCATTAT TTTATGAAAA CTCAAAGCTT GATGAAGTTC CTAAAGTGTA TAGTTGTATG 
AACAACAAGT TAGAAAAGCT ACTAGGAGAT ATAATTGCTT GTCTACATGT CAGCTACACT 
GGAACTTACG ACAGTGAACT TCTTGAACAG ATCTCACCAT TATTATGCAT AATATTTTCA 
CACAAGAACA AACAGATAAG AAAACAAAGT GCTCAGTTCT GGAATGCCAC ATTTGCTAAA 
GTGACGATGT TGATTTATCC TGAGGAGTTA AAGCCAATAC TGAAACAAGC CAAACAAAAA 
TCTCTGCTTT TATTGCCTGG TTTGGAAAAT GTTGAAATTA TGGAGGAATC CAGTGGACCA 
TATTCAGATA TGACAGAAAA TTCCCAATTA AATATGAAGA TAAGTGGCAT GGAAAGAAAA 
TCAAGTGGAA AAAGAGATTC TTTTTTGGCA CAAACAAAGG ATACAAAAGA CAGTATGAAA 
CCACCAGCTA AATTGAAACT AGATTCTTCA ACTCCAAAAG CAAAGGGTGA AATGCCTTTG 
GAAGAAGAAA AGTCTACTGA CTTTGTGTTT ATACCTCCAG AAGTAAAAGA AACAAAGGAA 
AGAATACTAA CTGAACACCA GAAAGAAGTA CTCAAAACAA AGAGGTGTGA TATTCCTGCC 
ATGTATAATA ATCTGGATGT TTCACAAGAT ACTTTATTTT CTCAGTATAG TCAGGAAGAA 
TCTATGGAAA TTTCTACTTT AATTGAAAAA TCAAAAGAAG ACTCCAAGAT GATATTTAAG 
GAGGAACAAA AGGAGAATGA CATTGTCGTT CCTCAACATG TCATGGAAAA CTGCGGTATG 
GATGAACATC TTGAAAAGGC TTCCCTTTTG AATAATGAGT GTGGTTCTAT TGAGGAAACC 
AGTCCAAAAA TACTGAGCAG TAATAATGAT GCCAGAAAAA AAGCTTTAAT TGCTTCAAAT 
AAACCAACAG AATGTGCATC TACTACAGAA AATCCATCTG GTGTCAGCAG TAGCTCGGTT 
TCTATTGCCA CCATTTCTGG AGCTCCCTTA CAGCCTACAA GTCGAAGGCA ATCCTTTATT 
ACTTTGGAAA AGTTTGATGG TTCAGAAAAT AGACCTTTTA GTCCATCACC CTTGAATAAT 
ATGTCATCAA CTGTAATAGT GAAAAGTAAC CAGGAAGGCA TGGGTAAAAC AGATAATCCA 
TCAAAAGCAA AGAAGAGAGA AGTGGCTCTT TCCAAATCTG ACTCTGAAAA AATAACACAT 
GGGACTAAGA GATCAAGCCG AAGGTCGAGT AAATCTGAAC CAATAGGGAG TAAAAAGTCT 
AAACCCTTAA TGAGATCTGA ACAAGAGAAA AGTACTCAAG AATCTGTTGA TGGCACAGTA 
GCCTTAGAAA ATAACCCACC TGGCTTGCTT AATCAGACAG AATGTGTGTT AGATAATCAG 
GTTCATCTCT CTGAATCTAC AGTGGAGTTT GAGGATACAA TGCTTGAATC AACAGTGGAG 
AATACTGTAT TAGAAAACAA TACTGTAGAA GAGAGAACCT TAGAAGTTAA CTTGGAATCC 
AAAGAAAACA CACCCCCAGC CATAATAACG GATCAGACAG TAAATGAGGA TAGTCATATT 
CAGGTAACTC CAAATCAGAA AACCCTTAGG CGATCTTTAA GGCGACGTTC AGAAACAGCA 
GAACCAACCA CTGATAGCCA AGATAAAGAA AATAATCATC AAAAAAGGGA AAGACGTAAA 
GAAGAAGAAA AGACTCCGCA GAAGAGTCCG TTACATGTAA AAGATGATGT TTTACCTAAG 
CAAAAATTGA TTTCTGAACA AAGTGTACAG GGAAACTTAG TTGACAAAGG AAATAATTTA 
CATGAGAAGG CTTTTGGGGA GACCAGTGCT AATGCTGAAA TTGATGAAAA TAGAAGAAAG 
CCAGATCTTG AGAATACTAA ATCTGAAGGA GATGGTGTGC CGGACATTGC AGATAAATCC 
TCTGAGAAAC CAGTAAGGGG ACGAACACGA TACCAAACAA GAAGAGCATC CCAGGGTTTA 
CTTTCCAGCA TTGAAAACTC AGAATCTGAT AGTTCTGAGG CAAAAGAAGA AGGTTCTAAA 
AAGAAAAGAT CTGGAAAATG GAAGAACAAA AGCAGTGACA ATGTTGACAT GGAAGATCAA 
GAAGAGAAAA TGGTAAAACA GGAATGTATA AAAATTGAAA ATCAGGCACA TGATTATAAA 
GGGAATCCTG AAGTAGACAA AGATATAAAA TCTCAAATCT GTGAAAAAGG AGATGAAAGT 
ACTACTGTAA GTCTTCAAGA TTCTACCGTA TCTCCAGATT TATTACAAGT TTATGATGAT 
GTATTGAATA CTTCTGAGGG AGAAAGTAAA AGTAACAAAG ATGCAGACCA TTCCTTTTCA 
AATCCTTCTG TACCAGAATC AAATCTAAGG ACTAGAAATG CCAGTAAGAG GTTACATAAG 
CGAGATTCTA TAGAAAATAG CATGGGGGAA TCCTCAAAAA TAGGGACATC AGACATTTCT 
TTGCTTTCTG AAAAAACTCT TCAGACAGTA GAATGCCAGC ACAAGAGAAG TAGGAGGGTG 
AGGCGATCTA AAGGTTGTGA TTGTTGTGGA GAAAAATCAC AGTCTCAGGA AAAGTCATTT 
ACTGGGTTAA AGGATACAGA AAATTACGAT GTAAAAGTCA GTGAAACAAA AAAGACAGAT 
GGGGAAACAC CTGTATCTAT TTCAGAAACT TCGAAAGCTG ATCTGTCCTC GGAGGTTAAA 
CTTTTAGATG AGCATCATAG TGTGAATTTT CATTTGGATC TCAAGGAAGA GAATGATACT 
ACTAATGATT CATTAATTAT ATCTGAAACT GAGTTAAAAG AAAATACTAC TCAAAGCTTT 
CTTCCTTCTG AAATGGTAAA ATTTAAAGAA ACTTCTCGTA GGGATTCTGA GGAAGCAATA 
TCTCTAGAAA CCCAGGAGCC ATCTAATAAG AAATGTAAAA CTGTTGACCC ACATTTAGAT 
GTTTCCAAAG ATATTTCACT GAAATGTTTT TCTTTGGAGA CTAAAGAAGA AAAGTCAGAG 
GCCACTTCAA AAGAGAAATT GAGTACAGAG AACATTGTTA TTGTTGAATC AAATGCAGAA 
TCAAATCCAG AAGTAGATGC TATGGAATTG AATGTAGAAA ATGATCAGTC TGAAGCTGGC 
TTCTCTGAAC AAAAGAATGT CAAAACTGGT ATTCCAGGGA AAGAAGTAAC AGAGGAAAAC 
AGTGAAAGAA TGGATACACC TGAAAAACTG AAAGCCAATG AAGCAGTAAC TGAGGAATTT 
AATTCAGCTA CTGATTATTC GGATACTCCG ACATCTGTAA AAGTGAATGC TCAGGTAGAG 
ATCTCTGAAC AAATAGCGGT TGGGGAGCTA GGTGGAGGAA GTGATGAATC TGATCCAGGC 
TCATCTGAAG AAATGAACGC TGAAATGAAA AATTGTGAAA AAACAGCGGT TTGTGAGGTG 
ACTACTGAAT CTGACCACGT CACTGAAACC GAAGACGAAG ATGTAACTAC CACGGCTTCT 
CTGCCTATAG AAGTTGAAAC AAAGGAATTA GATGTAGAAG TCAATAACTT TGTTCCCGAC 
ACACTTGAGA TGATCACTGA AGAAGGAAAG GTTGACCCTA ACAAAACTGA AACGAATACT 
GAACCTAATA AATTGGAGGA AACACAGTTA GATGACAATC AGATGGTAGT GGAAAGTAAT 
GAAACTTTAC CGGAAGTTCA CCATACTTCA GAGAAAGTAG AGGAGATAGC ACAACCTCTG 
ACATCTGAAA CAGATATTTC TGGACTAATA TCAGAAGACA GTAATACATC TCCTCAAAAA 
TCGAAGGATA TTGATCCTTT ACTCATGTCA GCAAACGAGA GTCCTAGTGG CATGCACACA 
CGTTGTGTGT GGTCTCCTTT GGCTTCTCCA TCCACCAGCA TTTTAAAGAG AGGACTAAAA 
AGACCCCAAG AAGATGAAAT CTCGTCACCT GTCCACAAGC ATAATACCAC TTCAGCCAAA 
GGATTTCTGT CCCCAGGATC ACGTAGCTCT AAATTTAAGA GCTCAAAGAA GTGTTTAATT 
GCAGAAATGG CTAAGGAATC TGTGCCATGC CCAACAGAAA GTGTTTACCC TGCTTTGGTG 
AACTGTGTAG CACCAGTTGA AATCATTTTA CCTCAGATTA CATCCAACAT GTGGGCAAGA 
GGACTGGGAC AGCTCATTAG AGCCAAGAAT ATAAAAACAA TTGGCGATTT GAGTACCCTT 
ACAGCATCTG AAATAAAAAC TCTTCCTATC CGTTCTCCAA AAGTGTCTAA CGTTAAAAAG 
GCTCTCAGAA TATATCATGA GCAGCAGGTG AAGTCTCGTG GACTAGAAGA GATTCCAGTT 
TTTGATATTT CTGAAAAAAC AATAAATGGA ATGGAAAATA AATCTATCTC TCCTGATGAA 
GAAAGACTTG CCTCAGATTT AATTGATCCT GTTGCTTTAG AAGCTCCGTT ATCTAAAAAT 
CTTGTGGCAC AGATTAGCGC ACTTGCTCTT CAACTAGATT CAGAAGATCT CTATAATTAT 
TCAGGAAGCC AGCTATTTGA AATGCACGAG AAACTTGGTA GCATGGCAAA CTCTATAATT 
AAAAATCTGC AGTCACGTTG GAGGTCACCA GCCCATGAAA ATTCTTTTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RIF1 ( XM_010802075.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010800377.1 Bos taurus replication timing regulatory factor 1 (RIF1), transcript variant X4, mRNA.

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CloneID	OBa55067
Clone ID Related Accession (Same CDS sequence)	XM_010802075.2 , XM_010802075.3 , XM_010826491.2
Accession Version	XM_010802075.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7251bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	telomere-associated protein RIF1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010802075.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGCCG CGGATCAGAG CCCCCTGGCA CCGCTGTTGG AGACTTTGGA AGACCCTTCC 
GCCTCCCTTG GAGAGCAGAC CGACGCTTAC CTGACCCTGA CCAGTCGTAT GACTGGAGAA 
GATGGAAAAG AAATCATTAC AGAAATTGAG AAAAAACTTC CTCAGCTATT TAAAGTTTTA 
AAGACTCACA TTGCCAGTCA AAACTCAGAG CTCAGTAGTG CTGCTCTACA GGCCTTGGGG 
TTTTGCTTAT ATAATCCCAA AATTGCCTCT GGATTATCAG AGATAAATAT TCAAGAACTG 
CTTTCAAAAC TGAATGATAT TGTTAAAAGT TCAGATAAAA ATGTACGTAC TAGGGCACTT 
TGGGTGATAT CCAAGCAGAC GTTTCCCACT GAAGTCGTTG GCAAAGTAGT TTCTAGTATC 
ATTGACTCAC TAGAAATAGT ATTTAATAGA GGAGAGATGC TTTCTGCTGT TGTGGATTAT 
GAAGCATTAA ATGTCATTAT AAGACTAATT GAACAAGCCC CAGTTCAAAT GGGAGAAGAG 
TCAATTAGGT GGGCAAAATT GGTCATACCT TTACTGGTTC ATTCAGCCCA AAAAGTACAT 
TTGCGGGGAG CCACTGCTCT AGAGATGGGA ATGCCTTTGT TGCTTCAGAA ACAGCAAGAA 
ATAGCATCTA TCACAGAGCA GCTTATGACT ACCAAATTAC TTTCAGAACT CCAGAAACTG 
TTTATGAGTA AAAATGAGAC TTATGTGTTA AAATTGTGGC CTTTATTTGT CAAACTTCTT 
GGGAAGACCT TGCATCGAAG TGGGAGTTTC ATTAATTCTC TGTTACAGCT GGAAGAACTG 
GGATTTCGTA GTGGAGCACC CATGATTAAA AAAATAGCTT TTATTGCTTG GAAGAGTTTA 
ATAGATAATT TTGCTTTAAA TCCAGAAATA CTGTGTAGTG CAAAAAGACT GAAGTTGTTG 
ATGCAGCCTT TGAGTTCTAT CCATGTGAGA ACAGAAACTC TAGCGCTGAC AAAACTAGAA 
GTCTGGTGGT ATTTGTTGAT GAGACTAGGA CCTCATCTTC CTGCTAATTT TGAACAGGTT 
TGTGTGCCTC TGATTCAGAG CACAATAAGC ATTGATTCTA ATGCTTCACC TCAAGGGAGT 
TCATCTCGTG TAGCTACTAC TCCAGGTTTA AGCCCTATGA CCCCAGTACA CAAAGGTGCT 
TCTCCATATG GAACTCCTGT AACCCCTCGG ATGAACTTGA ATTCAAATTT TGGAATGGCC 
ACAATCCCCT CTATACAACT TTTGGGGCTT GAAATGTTGC TTCATTTTTT GTTGGGTCCA 
GAAGTCGTGA GTTTTGCTAA GCAAAACAAA CTTATACTGA GCCTAGAACC ACTTGAACAT 
CCATTAATCA GCAGCTCTTC TTTTTTCTCC AAACACTCAA ATACACTTAT CACTGCTGTT 
CATGATAGCT TTGTTGCAGT TGGAAAAGAT GCTTCTGATG TAGTTATTAA TGCTATTTGG 
AAGGAGTTAA TTAGCTTGGT GAAGTCAGTT ATTGAATCAG GTAACAAAAA AGAGAGACCA 
GGTTCTGAAG TTTTGACTCT CTTATTAAAA TCTTTGGAAA GCATAGTGAA TTCAGAAGTA 
TTTCCTGTAT TGAAAACACT GGTTCTCATG GAAATTACAA TTAAAGGACT TCCTCAGAAA 
GTATTAGGTT CTCCAGCATA TCAGGTTGCT AATATGGATA TTCTTAATGG AACCCCTGCT 
TTGTTCTTAA TTCAGTTAAC TTTCAATAAT AATCTATTGG AATGTGGTGT GGAAGACGAA 
AGGTTTTTTC TCAACCTGGA AACACTTGTG GGCTGTGTTC TTTCGGGTCC AACTTCACCA 
CTCGCTTTCA GTGACTCTGT CTTAAATGTT GTCAATCAAA ATGCAAAGCA GTTGGATAAC 
AAGGAGCATC TTTGGAGAAT GTGGAGTATT ATAGTCACCC CATTAACAGA ATTGATTAAT 
CAGACCAATG AAGTGAATCA AGGGGATGCC TTAGAACATA ATTTCAGTGC TATCTATGGT 
GCACTGATTT TGCCAATAAA CCATATTTTT CCAGCACAAA AAATTCCAGT GGTCACCATG 
AAGACCTTAC TTAAAACTTG GTCAGAATTA TATAGAGCAT TTGCTCGCTG TGCAGCTTTG 
GTGGCAACTG CGGAAGAGAA TTTATGCTGT GAGGAATTTT GTTCCAAGAT AATGTCCAGT 
TTGGAAGATG AAGGCCTTTC AAATTTATTG TTCTTGGATA GGATCACTCA TATTATTACT 
GTAATGGTTG ATTGCATCGA CTTTTCACCA TATAATATTA AATATCAACC CAAAACTAAA 
TCACCACAGA GACCTACAGA TTGGTCCAAA AAGAAGAAGG AACCTTTAGG GAAGTTGGCT 
TCTTTAATTA AACTTATTGT GAGAGTGATC TATTCTTTTC ACACACTGAG CCTCAAGGAA 
GTACATTCTG ATACTCTCCT CACTATTGGC AACTCAATCA CCAGCATTCT TTCCAATTTA 
CTTGGACATA TTTCTCTGCC TTCTATGATC CAGAAAATAT TTGCAGCTTT AACAAGACCT 
CTGGCATTAT TTTATGAAAA CTCAAAGCTT GATGAAGTTC CTAAAGTGTA TAGTTGTATG 
AACAACAAGT TAGAAAAGCT ACTAGGAGAT ATAATTGCTT GTCTACATGT CAGCTACACT 
GGAACTTACG ACAGTGAACT TCTTGAACAG ATCTCACCAT TATTATGCAT AATATTTTCA 
CACAAGAACA AACAGATAAG AAAACAAAGT GCTCAGTTCT GGAATGCCAC ATTTGCTAAA 
GTGACGATGT TGATTTATCC TGAGGAGTTA AAGCCAATAC TGAAACAAGC CAAACAAAAA 
TCTCTGCTTT TATTGCCTGG TTTGGAAAAT GTTGAAATTA TGGAGGAATC CAGTGGACCA 
TATTCAGATA TGACAGAAAA TTCCCAATTA AATATGAAGA TAAGTGGCAT GGAAAGAAAA 
TCAAGTGGAA AAAGAGATTC TTTTTTGGCA CAAACAAAGG ATACAAAAGA CAGTATGAAA 
CCACCAGCTA AATTGAAACT AGATTCTTCA ACTCCAAAAG CAAAGGGTGA AATGCCTTTG 
GAAGAAGAAA AGTCTACTGA CTTTGTGTTT ATACCTCCAG AAGTAAAAGA AACAAAGGAA 
AGAATACTAA CTGAACACCA GAAAGAAGTA CTCAAAACAA AGAGGTGTGA TATTCCTGCC 
ATGTATAATA ATCTGGATGT TTCACAAGAT ACTTTATTTT CTCAGTATAG TCAGGAAGAA 
TCTATGGAAA TTTCTACTTT AATTGAAAAA TCAAAAGAAG ACTCCAAGAT GATATTTAAG 
GAGGAACAAA AGGAGAATGA CATTGTCGTT CCTCAACATG TCATGGAAAA CTGCGGTATG 
GATGAACATC TTGAAAAGGC TTCCCTTTTG AATAATGAGT GTGGTTCTAT TGAGGAAACC 
AGTCCAAAAA TACTGAGCAG TAATAATGAT GCCAGAAAAA AAGCTTTAAT TGCTTCAAAT 
AAACCAACAG AATGTGCATC TACTACAGAA AATCCATCTG GTGTCAGCAG TAGCTCGGTT 
TCTATTGCCA CCATTTCTGG AGCTCCCTTA CAGCCTACAA GTCGAAGGCA ATCCTTTATT 
ACTTTGGAAA AGTTTGATGG TTCAGAAAAT AGACCTTTTA GTCCATCACC CTTGAATAAT 
ATGTCATCAA CTGTAATAGT GAAAAGTAAC CAGGAAGGCA TGGGTAAAAC AGATAATCCA 
TCAAAAGCAA AGAAGAGAGA AGTGGCTCTT TCCAAATCTG ACTCTGAAAA AATAACACAT 
GGGACTAAGA GATCAAGCCG AAGGTCGAGT AAATCTGAAC CAATAGGGAG TAAAAAGTCT 
AAACCCTTAA TGAGATCTGA ACAAGAGAAA AGTACTCAAG AATCTGTTGA TGGCACAGTA 
GCCTTAGAAA ATAACCCACC TGGCTTGCTT AATCAGACAG AATGTGTGTT AGATAATCAG 
GTTCATCTCT CTGAATCTAC AGTGGAGTTT GAGGATACAA TGCTTGAATC AACAGTGGAG 
AATACTGTAT TAGAAAACAA TACTGTAGAA GAGAGAACCT TAGAAGTTAA CTTGGAATCC 
AAAGAAAACA CACCCCCAGC CATAATAACG GATCAGACAG TAAATGAGGA TAGTCATATT 
CAGGTAACTC CAAATCAGAA AACCCTTAGG CGATCTTTAA GGCGACGTTC AGAAACAGCA 
GAACCAACCA CTGATAGCCA AGATAAAGAA AATAATCATC AAAAAAGGGA AAGACGTAAA 
GAAGAAGAAA AGACTCCGCA GAAGAGTCCG TTACATGTAA AAGATGATGT TTTACCTAAG 
CAAAAATTGA TTTCTGAACA AAGTGTACAG GGAAACTTAG TTGACAAAGG AAATAATTTA 
CATGAGAAGG CTTTTGGGGA GACCAGTGCT AATGCTGAAA TTGATGAAAA TAGAAGAAAG 
CCAGATCTTG AGAATACTAA ATCTGAAGGA GATGGTGTGC CGGACATTGC AGATAAATCC 
TCTGAGAAAC CAGTAAGGGG ACGAACACGA TACCAAACAA GAAGAGCATC CCAGGGTTTA 
CTTTCCAGCA TTGAAAACTC AGAATCTGAT AGTTCTGAGG CAAAAGAAGA AGGTTCTAAA 
AAGAAAAGAT CTGGAAAATG GAAGAACAAA AGCAGTGACA ATGTTGACAT GGAAGATCAA 
GAAGAGAAAA TGGTAAAACA GGAATGTATA AAAATTGAAA ATCAGGCACA TGATTATAAA 
GGGAATCCTG AAGTAGACAA AGATATAAAA TCTCAAATCT GTGAAAAAGG AGATGAAAGT 
ACTACTGTAA GTCTTCAAGA TTCTACCGTA TCTCCAGATT TATTACAAGT TTATGATGAT 
GTATTGAATA CTTCTGAGGG AGAAAGTAAA AGTAACAAAG ATGCAGACCA TTCCTTTTCA 
AATCCTTCTG TACCAGAATC AAATCTAAGG ACTAGAAATG CCAGTAAGAG GTTACATAAG 
CGAGATTCTA TAGAAAATAG CATGGGGGAA TCCTCAAAAA TAGGGACATC AGACATTTCT 
TTGCTTTCTG AAAAAACTCT TCAGACAGTA GAATGCCAGC ACAAGAGAAG TAGGAGGGTG 
AGGCGATCTA AAGGTTGTGA TTGTTGTGGA GAAAAATCAC AGTCTCAGGA AAAGTCATTT 
ACTGGGTTAA AGGATACAGA AAATTACGAT GTAAAAGTCA GTGAAACAAA AAAGACAGAT 
GGGGAAACAC CTGTATCTAT TTCAGAAACT TCGAAAGCTG ATCTGTCCTC GGAGGTTAAA 
CTTTTAGATG AGCATCATAG TGTGAATTTT CATTTGGATC TCAAGGAAGA GAATGATACT 
ACTAATGATT CATTAATTAT ATCTGAAACT GAGTTAAAAG AAAATACTAC TCAAAGCTTT 
CTTCCTTCTG AAATGGTAAA ATTTAAAGAA ACTTCTCGTA GGGATTCTGA GGAAGCAATA 
TCTCTAGAAA CCCAGGAGCC ATCTAATAAG AAATGTAAAA CTGTTGACCC ACATTTAGAT 
GTTTCCAAAG ATATTTCACT GAAATGTTTT TCTTTGGAGA CTAAAGAAGA AAAGTCAGAG 
GCCACTTCAA AAGAGAAATT GAGTACAGAG AACATTGTTA TTGTTGAATC AAATGCAGAA 
TCAAATCCAG AAGTAGATGC TATGGAATTG AATGTAGAAA ATGATCAGTC TGAAGCTGGC 
TTCTCTGAAC AAAAGAATGT CAAAACTGGT ATTCCAGGGA AAGAAGTAAC AGAGGAAAAC 
AGTGAAAGAA TGGATACACC TGAAAAACTG AAAGCCAATG AAGCAGTAAC TGAGGAATTT 
AATTCAGCTA CTGATTATTC GGATACTCCG ACATCTGTAA AAGTGAATGC TCAGGTAGAG 
ATCTCTGAAC AAATAGCGGT TGGGGAGCTA GGTGGAGGAA GTGATGAATC TGATCCAGGC 
TCATCTGAAG AAATGAACGC TGAAATGAAA AATTGTGAAA AAACAGCGGT TTGTGAGGTG 
ACTACTGAAT CTGACCACGT CACTGAAACC GAAGACGAAG ATGTAACTAC CACGGCTTCT 
CTGCCTATAG AAGTTGAAAC AAAGGAATTA GATGTAGAAG TCAATAACTT TGTTCCCGAC 
ACACTTGAGA TGATCACTGA AGAAGGAAAG GTTGACCCTA ACAAAACTGA AACGAATACT 
GAACCTAATA AATTGGAGGA AACACAGTTA GATGACAATC AGATGGTAGT GGAAAGTAAT 
GAAACTTTAC CGGAAGTTCA CCATACTTCA GAGAAAGTAG AGGAGATAGC ACAACCTCTG 
ACATCTGAAA CAGATATTTC TGGACTAATA TCAGAAGACA GTAATACATC TCCTCAAAAA 
TCGAAGGATA TTGATCCTTT ACTCATGTCA GCAAACGAGA GTCCTAGTGG CATGCACACA 
CGTTGTGTGT GGTCTCCTTT GGCTTCTCCA TCCACCAGCA TTTTAAAGAG AGGACTAAAA 
AGACCCCAAG AAGATGAAAT CTCGTCACCT GTCCACAAGC ATAATACCAC TTCAGCCAAA 
GGATTTCTGT CCCCAGGATC ACGTAGCTCT AAATTTAAGA GCTCAAAGAA GTGTTTAATT 
GCAGAAATGG CTAAGGAATC TGTGCCATGC CCAACAGAAA GTGTTTACCC TGCTTTGGTG 
AACTGTGTAG CACCAGTTGA AATCATTTTA CCTCAGATTA CATCCAACAT GTGGGCAAGA 
GGACTGGGAC AGCTCATTAG AGCCAAGAAT ATAAAAACAA TTGGCGATTT GAGTACCCTT 
ACAGCATCTG AAATAAAAAC TCTTCCTATC CGTTCTCCAA AAGTGTCTAA CGTTAAAAAG 
GCTCTCAGAA TATATCATGA GCAGCAGGTG AAGTCTCGTG GACTAGAAGA GATTCCAGTT 
TTTGATATTT CTGAAAAAAC AATAAATGGA ATGGAAAATA AATCTATCTC TCCTGATGAA 
GAAAGACTTG CCTCAGATTT AATTGATCCT GTTGCTTTAG AAGCTCCGTT ATCTAAAAAT 
CTTGTGGCAC AGATTAGCGC ACTTGCTCTT CAACTAGATT CAGAAGATCT CTATAATTAT 
TCAGGAAGCC AGCTATTTGA AATGCACGAG AAACTTGGTA GCATGGCAAA CTCTATAATT 
AAAAATCTGC AGTCACGTTG GAGGTCACCA GCCCATGAAA ATTCTTTTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010800377.1
CDS	171..7421
Translation	MTAADQSPLAPLLETLEDPSASLGEQTDAYLTLTSRMTGEDGKEIITEIEKKLPQLFKVLKTHIASQNSELSSAALQALGFCLYNPKIASGLSEINIQELLSKLNDIVKSSDKNVRTRALWVISKQTFPTEVVGKVVSSIIDSLEIVFNRGEMLSAVVDYEALNVIIRLIEQAPVQMGEESIRWAKLVIPLLVHSAQKVHLRGATALEMGMPLLLQKQQEIASITEQLMTTKLLSELQKLFMSKNETYVLKLWPLFVKLLGKTLHRSGSFINSLLQLEELGFRSGAPMIKKIAFIAWKSLIDNFALNPEILCSAKRLKLLMQPLSSIHVRTETLALTKLEVWWYLLMRLGPHLPANFEQVCVPLIQSTISIDSNASPQGSSSRVATTPGLSPMTPVHKGASPYGTPVTPRMNLNSNFGMATIPSIQLLGLEMLLHFLLGPEVVSFAKQNKLILSLEPLEHPLISSSSFFSKHSNTLITAVHDSFVAVGKDASDVVINAIWKELISLVKSVIESGNKKERPGSEVLTLLLKSLESIVNSEVFPVLKTLVLMEITIKGLPQKVLGSPAYQVANMDILNGTPALFLIQLTFNNNLLECGVEDERFFLNLETLVGCVLSGPTSPLAFSDSVLNVVNQNAKQLDNKEHLWRMWSIIVTPLTELINQTNEVNQGDALEHNFSAIYGALILPINHIFPAQKIPVVTMKTLLKTWSELYRAFARCAALVATAEENLCCEEFCSKIMSSLEDEGLSNLLFLDRITHIITVMVDCIDFSPYNIKYQPKTKSPQRPTDWSKKKKEPLGKLASLIKLIVRVIYSFHTLSLKEVHSDTLLTIGNSITSILSNLLGHISLPSMIQKIFAALTRPLALFYENSKLDEVPKVYSCMNNKLEKLLGDIIACLHVSYTGTYDSELLEQISPLLCIIFSHKNKQIRKQSAQFWNATFAKVTMLIYPEELKPILKQAKQKSLLLLPGLENVEIMEESSGPYSDMTENSQLNMKISGMERKSSGKRDSFLAQTKDTKDSMKPPAKLKLDSSTPKAKGEMPLEEEKSTDFVFIPPEVKETKERILTEHQKEVLKTKRCDIPAMYNNLDVSQDTLFSQYSQEESMEISTLIEKSKEDSKMIFKEEQKENDIVVPQHVMENCGMDEHLEKASLLNNECGSIEETSPKILSSNNDARKKALIASNKPTECASTTENPSGVSSSSVSIATISGAPLQPTSRRQSFITLEKFDGSENRPFSPSPLNNMSSTVIVKSNQEGMGKTDNPSKAKKREVALSKSDSEKITHGTKRSSRRSSKSEPIGSKKSKPLMRSEQEKSTQESVDGTVALENNPPGLLNQTECVLDNQVHLSESTVEFEDTMLESTVENTVLENNTVEERTLEVNLESKENTPPAIITDQTVNEDSHIQVTPNQKTLRRSLRRRSETAEPTTDSQDKENNHQKRERRKEEEKTPQKSPLHVKDDVLPKQKLISEQSVQGNLVDKGNNLHEKAFGETSANAEIDENRRKPDLENTKSEGDGVPDIADKSSEKPVRGRTRYQTRRASQGLLSSIENSESDSSEAKEEGSKKKRSGKWKNKSSDNVDMEDQEEKMVKQECIKIENQAHDYKGNPEVDKDIKSQICEKGDESTTVSLQDSTVSPDLLQVYDDVLNTSEGESKSNKDADHSFSNPSVPESNLRTRNASKRLHKRDSIENSMGESSKIGTSDISLLSEKTLQTVECQHKRSRRVRRSKGCDCCGEKSQSQEKSFTGLKDTENYDVKVSETKKTDGETPVSISETSKADLSSEVKLLDEHHSVNFHLDLKEENDTTNDSLIISETELKENTTQSFLPSEMVKFKETSRRDSEEAISLETQEPSNKKCKTVDPHLDVSKDISLKCFSLETKEEKSEATSKEKLSTENIVIVESNAESNPEVDAMELNVENDQSEAGFSEQKNVKTGIPGKEVTEENSERMDTPEKLKANEAVTEEFNSATDYSDTPTSVKVNAQVEISEQIAVGELGGGSDESDPGSSEEMNAEMKNCEKTAVCEVTTESDHVTETEDEDVTTTASLPIEVETKELDVEVNNFVPDTLEMITEEGKVDPNKTETNTEPNKLEETQLDDNQMVVESNETLPEVHHTSEKVEEIAQPLTSETDISGLISEDSNTSPQKSKDIDPLLMSANESPSGMHTRCVWSPLASPSTSILKRGLKRPQEDEISSPVHKHNTTSAKGFLSPGSRSSKFKSSKKCLIAEMAKESVPCPTESVYPALVNCVAPVEIILPQITSNMWARGLGQLIRAKNIKTIGDLSTLTASEIKTLPIRSPKVSNVKKALRIYHEQQVKSRGLEEIPVFDISEKTINGMENKSISPDEERLASDLIDPVALEAPLSKNLVAQISALALQLDSEDLYNYSGSQLFEMHEKLGSMANSIIKNLQSRWRSPAHENSF

Target ORF information:

RefSeq Version	XM_010802075.3
Organism	Bos taurus(cattle)
Definition	Bos taurus replication timing regulatory factor 1 (RIF1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010802075.3

ORF Insert Sequence:

ATGACGGCCG CGGATCAGAG CCCCCTGGCA CCGCTGTTGG AGACTTTGGA AGACCCTTCC 
GCCTCCCTTG GAGAGCAGAC CGACGCTTAC CTGACCCTGA CCAGTCGTAT GACTGGAGAA 
GATGGAAAAG AAATCATTAC AGAAATTGAG AAAAAACTTC CTCAGCTATT TAAAGTTTTA 
AAGACTCACA TTGCCAGTCA AAACTCAGAG CTCAGTAGTG CTGCTCTACA GGCCTTGGGG 
TTTTGCTTAT ATAATCCCAA AATTGCCTCT GGATTATCAG AGATAAATAT TCAAGAACTG 
CTTTCAAAAC TGAATGATAT TGTTAAAAGT TCAGATAAAA ATGTACGTAC TAGGGCACTT 
TGGGTGATAT CCAAGCAGAC GTTTCCCACT GAAGTCGTTG GCAAAGTAGT TTCTAGTATC 
ATTGACTCAC TAGAAATAGT ATTTAATAGA GGAGAGATGC TTTCTGCTGT TGTGGATTAT 
GAAGCATTAA ATGTCATTAT AAGACTAATT GAACAAGCCC CAGTTCAAAT GGGAGAAGAG 
TCAATTAGGT GGGCAAAATT GGTCATACCT TTACTGGTTC ATTCAGCCCA AAAAGTACAT 
TTGCGGGGAG CCACTGCTCT AGAGATGGGA ATGCCTTTGT TGCTTCAGAA ACAGCAAGAA 
ATAGCATCTA TCACAGAGCA GCTTATGACT ACCAAATTAC TTTCAGAACT CCAGAAACTG 
TTTATGAGTA AAAATGAGAC TTATGTGTTA AAATTGTGGC CTTTATTTGT CAAACTTCTT 
GGGAAGACCT TGCATCGAAG TGGGAGTTTC ATTAATTCTC TGTTACAGCT GGAAGAACTG 
GGATTTCGTA GTGGAGCACC CATGATTAAA AAAATAGCTT TTATTGCTTG GAAGAGTTTA 
ATAGATAATT TTGCTTTAAA TCCAGAAATA CTGTGTAGTG CAAAAAGACT GAAGTTGTTG 
ATGCAGCCTT TGAGTTCTAT CCATGTGAGA ACAGAAACTC TAGCGCTGAC AAAACTAGAA 
GTCTGGTGGT ATTTGTTGAT GAGACTAGGA CCTCATCTTC CTGCTAATTT TGAACAGGTT 
TGTGTGCCTC TGATTCAGAG CACAATAAGC ATTGATTCTA ATGCTTCACC TCAAGGGAGT 
TCATCTCGTG TAGCTACTAC TCCAGGTTTA AGCCCTATGA CCCCAGTACA CAAAGGTGCT 
TCTCCATATG GAACTCCTGT AACCCCTCGG ATGAACTTGA ATTCAAATTT TGGAATGGCC 
ACAATCCCCT CTATACAACT TTTGGGGCTT GAAATGTTGC TTCATTTTTT GTTGGGTCCA 
GAAGTCGTGA GTTTTGCTAA GCAAAACAAA CTTATACTGA GCCTAGAACC ACTTGAACAT 
CCATTAATCA GCAGCTCTTC TTTTTTCTCC AAACACTCAA ATACACTTAT CACTGCTGTT 
CATGATAGCT TTGTTGCAGT TGGAAAAGAT GCTTCTGATG TAGTTATTAA TGCTATTTGG 
AAGGAGTTAA TTAGCTTGGT GAAGTCAGTT ATTGAATCAG GTAACAAAAA AGAGAGACCA 
GGTTCTGAAG TTTTGACTCT CTTATTAAAA TCTTTGGAAA GCATAGTGAA TTCAGAAGTA 
TTTCCTGTAT TGAAAACACT GGTTCTCATG GAAATTACAA TTAAAGGACT TCCTCAGAAA 
GTATTAGGTT CTCCAGCATA TCAGGTTGCT AATATGGATA TTCTTAATGG AACCCCTGCT 
TTGTTCTTAA TTCAGTTAAC TTTCAATAAT AATCTATTGG AATGTGGTGT GGAAGACGAA 
AGGTTTTTTC TCAACCTGGA AACACTTGTG GGCTGTGTTC TTTCGGGTCC AACTTCACCA 
CTCGCTTTCA GTGACTCTGT CTTAAATGTT GTCAATCAAA ATGCAAAGCA GTTGGATAAC 
AAGGAGCATC TTTGGAGAAT GTGGAGTATT ATAGTCACCC CATTAACAGA ATTGATTAAT 
CAGACCAATG AAGTGAATCA AGGGGATGCC TTAGAACATA ATTTCAGTGC TATCTATGGT 
GCACTGATTT TGCCAATAAA CCATATTTTT CCAGCACAAA AAATTCCAGT GGTCACCATG 
AAGACCTTAC TTAAAACTTG GTCAGAATTA TATAGAGCAT TTGCTCGCTG TGCAGCTTTG 
GTGGCAACTG CGGAAGAGAA TTTATGCTGT GAGGAATTTT GTTCCAAGAT AATGTCCAGT 
TTGGAAGATG AAGGCCTTTC AAATTTATTG TTCTTGGATA GGATCACTCA TATTATTACT 
GTAATGGTTG ATTGCATCGA CTTTTCACCA TATAATATTA AATATCAACC CAAAACTAAA 
TCACCACAGA GACCTACAGA TTGGTCCAAA AAGAAGAAGG AACCTTTAGG GAAGTTGGCT 
TCTTTAATTA AACTTATTGT GAGAGTGATC TATTCTTTTC ACACACTGAG CCTCAAGGAA 
GTACATTCTG ATACTCTCCT CACTATTGGC AACTCAATCA CCAGCATTCT TTCCAATTTA 
CTTGGACATA TTTCTCTGCC TTCTATGATC CAGAAAATAT TTGCAGCTTT AACAAGACCT 
CTGGCATTAT TTTATGAAAA CTCAAAGCTT GATGAAGTTC CTAAAGTGTA TAGTTGTATG 
AACAACAAGT TAGAAAAGCT ACTAGGAGAT ATAATTGCTT GTCTACATGT CAGCTACACT 
GGAACTTACG ACAGTGAACT TCTTGAACAG ATCTCACCAT TATTATGCAT AATATTTTCA 
CACAAGAACA AACAGATAAG AAAACAAAGT GCTCAGTTCT GGAATGCCAC ATTTGCTAAA 
GTGACGATGT TGATTTATCC TGAGGAGTTA AAGCCAATAC TGAAACAAGC CAAACAAAAA 
TCTCTGCTTT TATTGCCTGG TTTGGAAAAT GTTGAAATTA TGGAGGAATC CAGTGGACCA 
TATTCAGATA TGACAGAAAA TTCCCAATTA AATATGAAGA TAAGTGGCAT GGAAAGAAAA 
TCAAGTGGAA AAAGAGATTC TTTTTTGGCA CAAACAAAGG ATACAAAAGA CAGTATGAAA 
CCACCAGCTA AATTGAAACT AGATTCTTCA ACTCCAAAAG CAAAGGGTGA AATGCCTTTG 
GAAGAAGAAA AGTCTACTGA CTTTGTGTTT ATACCTCCAG AAGTAAAAGA AACAAAGGAA 
AGAATACTAA CTGAACACCA GAAAGAAGTA CTCAAAACAA AGAGGTGTGA TATTCCTGCC 
ATGTATAATA ATCTGGATGT TTCACAAGAT ACTTTATTTT CTCAGTATAG TCAGGAAGAA 
TCTATGGAAA TTTCTACTTT AATTGAAAAA TCAAAAGAAG ACTCCAAGAT GATATTTAAG 
GAGGAACAAA AGGAGAATGA CATTGTCGTT CCTCAACATG TCATGGAAAA CTGCGGTATG 
GATGAACATC TTGAAAAGGC TTCCCTTTTG AATAATGAGT GTGGTTCTAT TGAGGAAACC 
AGTCCAAAAA TACTGAGCAG TAATAATGAT GCCAGAAAAA AAGCTTTAAT TGCTTCAAAT 
AAACCAACAG AATGTGCATC TACTACAGAA AATCCATCTG GTGTCAGCAG TAGCTCGGTT 
TCTATTGCCA CCATTTCTGG AGCTCCCTTA CAGCCTACAA GTCGAAGGCA ATCCTTTATT 
ACTTTGGAAA AGTTTGATGG TTCAGAAAAT AGACCTTTTA GTCCATCACC CTTGAATAAT 
ATGTCATCAA CTGTAATAGT GAAAAGTAAC CAGGAAGGCA TGGGTAAAAC AGATAATCCA 
TCAAAAGCAA AGAAGAGAGA AGTGGCTCTT TCCAAATCTG ACTCTGAAAA AATAACACAT 
GGGACTAAGA GATCAAGCCG AAGGTCGAGT AAATCTGAAC CAATAGGGAG TAAAAAGTCT 
AAACCCTTAA TGAGATCTGA ACAAGAGAAA AGTACTCAAG AATCTGTTGA TGGCACAGTA 
GCCTTAGAAA ATAACCCACC TGGCTTGCTT AATCAGACAG AATGTGTGTT AGATAATCAG 
GTTCATCTCT CTGAATCTAC AGTGGAGTTT GAGGATACAA TGCTTGAATC AACAGTGGAG 
AATACTGTAT TAGAAAACAA TACTGTAGAA GAGAGAACCT TAGAAGTTAA CTTGGAATCC 
AAAGAAAACA CACCCCCAGC CATAATAACG GATCAGACAG TAAATGAGGA TAGTCATATT 
CAGGTAACTC CAAATCAGAA AACCCTTAGG CGATCTTTAA GGCGACGTTC AGAAACAGCA 
GAACCAACCA CTGATAGCCA AGATAAAGAA AATAATCATC AAAAAAGGGA AAGACGTAAA 
GAAGAAGAAA AGACTCCGCA GAAGAGTCCG TTACATGTAA AAGATGATGT TTTACCTAAG 
CAAAAATTGA TTTCTGAACA AAGTGTACAG GGAAACTTAG TTGACAAAGG AAATAATTTA 
CATGAGAAGG CTTTTGGGGA GACCAGTGCT AATGCTGAAA TTGATGAAAA TAGAAGAAAG 
CCAGATCTTG AGAATACTAA ATCTGAAGGA GATGGTGTGC CGGACATTGC AGATAAATCC 
TCTGAGAAAC CAGTAAGGGG ACGAACACGA TACCAAACAA GAAGAGCATC CCAGGGTTTA 
CTTTCCAGCA TTGAAAACTC AGAATCTGAT AGTTCTGAGG CAAAAGAAGA AGGTTCTAAA 
AAGAAAAGAT CTGGAAAATG GAAGAACAAA AGCAGTGACA ATGTTGACAT GGAAGATCAA 
GAAGAGAAAA TGGTAAAACA GGAATGTATA AAAATTGAAA ATCAGGCACA TGATTATAAA 
GGGAATCCTG AAGTAGACAA AGATATAAAA TCTCAAATCT GTGAAAAAGG AGATGAAAGT 
ACTACTGTAA GTCTTCAAGA TTCTACCGTA TCTCCAGATT TATTACAAGT TTATGATGAT 
GTATTGAATA CTTCTGAGGG AGAAAGTAAA AGTAACAAAG ATGCAGACCA TTCCTTTTCA 
AATCCTTCTG TACCAGAATC AAATCTAAGG ACTAGAAATG CCAGTAAGAG GTTACATAAG 
CGAGATTCTA TAGAAAATAG CATGGGGGAA TCCTCAAAAA TAGGGACATC AGACATTTCT 
TTGCTTTCTG AAAAAACTCT TCAGACAGTA GAATGCCAGC ACAAGAGAAG TAGGAGGGTG 
AGGCGATCTA AAGGTTGTGA TTGTTGTGGA GAAAAATCAC AGTCTCAGGA AAAGTCATTT 
ACTGGGTTAA AGGATACAGA AAATTACGAT GTAAAAGTCA GTGAAACAAA AAAGACAGAT 
GGGGAAACAC CTGTATCTAT TTCAGAAACT TCGAAAGCTG ATCTGTCCTC GGAGGTTAAA 
CTTTTAGATG AGCATCATAG TGTGAATTTT CATTTGGATC TCAAGGAAGA GAATGATACT 
ACTAATGATT CATTAATTAT ATCTGAAACT GAGTTAAAAG AAAATACTAC TCAAAGCTTT 
CTTCCTTCTG AAATGGTAAA ATTTAAAGAA ACTTCTCGTA GGGATTCTGA GGAAGCAATA 
TCTCTAGAAA CCCAGGAGCC ATCTAATAAG AAATGTAAAA CTGTTGACCC ACATTTAGAT 
GTTTCCAAAG ATATTTCACT GAAATGTTTT TCTTTGGAGA CTAAAGAAGA AAAGTCAGAG 
GCCACTTCAA AAGAGAAATT GAGTACAGAG AACATTGTTA TTGTTGAATC AAATGCAGAA 
TCAAATCCAG AAGTAGATGC TATGGAATTG AATGTAGAAA ATGATCAGTC TGAAGCTGGC 
TTCTCTGAAC AAAAGAATGT CAAAACTGGT ATTCCAGGGA AAGAAGTAAC AGAGGAAAAC 
AGTGAAAGAA TGGATACACC TGAAAAACTG AAAGCCAATG AAGCAGTAAC TGAGGAATTT 
AATTCAGCTA CTGATTATTC GGATACTCCG ACATCTGTAA AAGTGAATGC TCAGGTAGAG 
ATCTCTGAAC AAATAGCGGT TGGGGAGCTA GGTGGAGGAA GTGATGAATC TGATCCAGGC 
TCATCTGAAG AAATGAACGC TGAAATGAAA AATTGTGAAA AAACAGCGGT TTGTGAGGTG 
ACTACTGAAT CTGACCACGT CACTGAAACC GAAGACGAAG ATGTAACTAC CACGGCTTCT 
CTGCCTATAG AAGTTGAAAC AAAGGAATTA GATGTAGAAG TCAATAACTT TGTTCCCGAC 
ACACTTGAGA TGATCACTGA AGAAGGAAAG GTTGACCCTA ACAAAACTGA AACGAATACT 
GAACCTAATA AATTGGAGGA AACACAGTTA GATGACAATC AGATGGTAGT GGAAAGTAAT 
GAAACTTTAC CGGAAGTTCA CCATACTTCA GAGAAAGTAG AGGAGATAGC ACAACCTCTG 
ACATCTGAAA CAGATATTTC TGGACTAATA TCAGAAGACA GTAATACATC TCCTCAAAAA 
TCGAAGGATA TTGATCCTTT ACTCATGTCA GCAAACGAGA GTCCTAGTGG CATGCACACA 
CGTTGTGTGT GGTCTCCTTT GGCTTCTCCA TCCACCAGCA TTTTAAAGAG AGGACTAAAA 
AGACCCCAAG AAGATGAAAT CTCGTCACCT GTCCACAAGC ATAATACCAC TTCAGCCAAA 
GGATTTCTGT CCCCAGGATC ACGTAGCTCT AAATTTAAGA GCTCAAAGAA GTGTTTAATT 
GCAGAAATGG CTAAGGAATC TGTGCCATGC CCAACAGAAA GTGTTTACCC TGCTTTGGTG 
AACTGTGTAG CACCAGTTGA AATCATTTTA CCTCAGATTA CATCCAACAT GTGGGCAAGA 
GGACTGGGAC AGCTCATTAG AGCCAAGAAT ATAAAAACAA TTGGCGATTT GAGTACCCTT 
ACAGCATCTG AAATAAAAAC TCTTCCTATC CGTTCTCCAA AAGTGTCTAA CGTTAAAAAG 
GCTCTCAGAA TATATCATGA GCAGCAGGTG AAGTCTCGTG GACTAGAAGA GATTCCAGTT 
TTTGATATTT CTGAAAAAAC AATAAATGGA ATGGAAAATA AATCTATCTC TCCTGATGAA 
GAAAGACTTG CCTCAGATTT AATTGATCCT GTTGCTTTAG AAGCTCCGTT ATCTAAAAAT 
CTTGTGGCAC AGATTAGCGC ACTTGCTCTT CAACTAGATT CAGAAGATCT CTATAATTAT 
TCAGGAAGCC AGCTATTTGA AATGCACGAG AAACTTGGTA GCATGGCAAA CTCTATAATT 
AAAAATCTGC AGTCACGTTG GAGGTCACCA GCCCATGAAA ATTCTTTTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RIF1 ( XM_010826491.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010824793.1 Bos taurus replication timing regulatory factor 1 (RIF1), transcript variant X4, mRNA.

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CloneID	OBa55067
Clone ID Related Accession (Same CDS sequence)	XM_010802075.2 , XM_010802075.3 , XM_010826491.2
Accession Version	XM_010826491.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7251bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	telomere-associated protein RIF1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644591.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010826491.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGCCG CGGATCAGAG CCCCCTGGCA CCGCTGTTGG AGACTTTGGA AGACCCTTCC 
GCCTCCCTTG GAGAGCAGAC CGACGCTTAC CTGACCCTGA CCAGTCGTAT GACTGGAGAA 
GATGGAAAAG AAATCATTAC AGAAATTGAG AAAAAACTTC CTCAGCTATT TAAAGTTTTA 
AAGACTCACA TTGCCAGTCA AAACTCAGAG CTCAGTAGTG CTGCTCTACA GGCCTTGGGG 
TTTTGCTTAT ATAATCCCAA AATTGCCTCT GGATTATCAG AGATAAATAT TCAAGAACTG 
CTTTCAAAAC TGAATGATAT TGTTAAAAGT TCAGATAAAA ATGTACGTAC TAGGGCACTT 
TGGGTGATAT CCAAGCAGAC GTTTCCCACT GAAGTCGTTG GCAAAGTAGT TTCTAGTATC 
ATTGACTCAC TAGAAATAGT ATTTAATAGA GGAGAGATGC TTTCTGCTGT TGTGGATTAT 
GAAGCATTAA ATGTCATTAT AAGACTAATT GAACAAGCCC CAGTTCAAAT GGGAGAAGAG 
TCAATTAGGT GGGCAAAATT GGTCATACCT TTACTGGTTC ATTCAGCCCA AAAAGTACAT 
TTGCGGGGAG CCACTGCTCT AGAGATGGGA ATGCCTTTGT TGCTTCAGAA ACAGCAAGAA 
ATAGCATCTA TCACAGAGCA GCTTATGACT ACCAAATTAC TTTCAGAACT CCAGAAACTG 
TTTATGAGTA AAAATGAGAC TTATGTGTTA AAATTGTGGC CTTTATTTGT CAAACTTCTT 
GGGAAGACCT TGCATCGAAG TGGGAGTTTC ATTAATTCTC TGTTACAGCT GGAAGAACTG 
GGATTTCGTA GTGGAGCACC CATGATTAAA AAAATAGCTT TTATTGCTTG GAAGAGTTTA 
ATAGATAATT TTGCTTTAAA TCCAGAAATA CTGTGTAGTG CAAAAAGACT GAAGTTGTTG 
ATGCAGCCTT TGAGTTCTAT CCATGTGAGA ACAGAAACTC TAGCGCTGAC AAAACTAGAA 
GTCTGGTGGT ATTTGTTGAT GAGACTAGGA CCTCATCTTC CTGCTAATTT TGAACAGGTT 
TGTGTGCCTC TGATTCAGAG CACAATAAGC ATTGATTCTA ATGCTTCACC TCAAGGGAGT 
TCATCTCGTG TAGCTACTAC TCCAGGTTTA AGCCCTATGA CCCCAGTACA CAAAGGTGCT 
TCTCCATATG GAACTCCTGT AACCCCTCGG ATGAACTTGA ATTCAAATTT TGGAATGGCC 
ACAATCCCCT CTATACAACT TTTGGGGCTT GAAATGTTGC TTCATTTTTT GTTGGGTCCA 
GAAGTCGTGA GTTTTGCTAA GCAAAACAAA CTTATACTGA GCCTAGAACC ACTTGAACAT 
CCATTAATCA GCAGCTCTTC TTTTTTCTCC AAACACTCAA ATACACTTAT CACTGCTGTT 
CATGATAGCT TTGTTGCAGT TGGAAAAGAT GCTTCTGATG TAGTTATTAA TGCTATTTGG 
AAGGAGTTAA TTAGCTTGGT GAAGTCAGTT ATTGAATCAG GTAACAAAAA AGAGAGACCA 
GGTTCTGAAG TTTTGACTCT CTTATTAAAA TCTTTGGAAA GCATAGTGAA TTCAGAAGTA 
TTTCCTGTAT TGAAAACACT GGTTCTCATG GAAATTACAA TTAAAGGACT TCCTCAGAAA 
GTATTAGGTT CTCCAGCATA TCAGGTTGCT AATATGGATA TTCTTAATGG AACCCCTGCT 
TTGTTCTTAA TTCAGTTAAC TTTCAATAAT AATCTATTGG AATGTGGTGT GGAAGACGAA 
AGGTTTTTTC TCAACCTGGA AACACTTGTG GGCTGTGTTC TTTCGGGTCC AACTTCACCA 
CTCGCTTTCA GTGACTCTGT CTTAAATGTT GTCAATCAAA ATGCAAAGCA GTTGGATAAC 
AAGGAGCATC TTTGGAGAAT GTGGAGTATT ATAGTCACCC CATTAACAGA ATTGATTAAT 
CAGACCAATG AAGTGAATCA AGGGGATGCC TTAGAACATA ATTTCAGTGC TATCTATGGT 
GCACTGATTT TGCCAATAAA CCATATTTTT CCAGCACAAA AAATTCCAGT GGTCACCATG 
AAGACCTTAC TTAAAACTTG GTCAGAATTA TATAGAGCAT TTGCTCGCTG TGCAGCTTTG 
GTGGCAACTG CGGAAGAGAA TTTATGCTGT GAGGAATTTT GTTCCAAGAT AATGTCCAGT 
TTGGAAGATG AAGGCCTTTC AAATTTATTG TTCTTGGATA GGATCACTCA TATTATTACT 
GTAATGGTTG ATTGCATCGA CTTTTCACCA TATAATATTA AATATCAACC CAAAACTAAA 
TCACCACAGA GACCTACAGA TTGGTCCAAA AAGAAGAAGG AACCTTTAGG GAAGTTGGCT 
TCTTTAATTA AACTTATTGT GAGAGTGATC TATTCTTTTC ACACACTGAG CCTCAAGGAA 
GTACATTCTG ATACTCTCCT CACTATTGGC AACTCAATCA CCAGCATTCT TTCCAATTTA 
CTTGGACATA TTTCTCTGCC TTCTATGATC CAGAAAATAT TTGCAGCTTT AACAAGACCT 
CTGGCATTAT TTTATGAAAA CTCAAAGCTT GATGAAGTTC CTAAAGTGTA TAGTTGTATG 
AACAACAAGT TAGAAAAGCT ACTAGGAGAT ATAATTGCTT GTCTACATGT CAGCTACACT 
GGAACTTACG ACAGTGAACT TCTTGAACAG ATCTCACCAT TATTATGCAT AATATTTTCA 
CACAAGAACA AACAGATAAG AAAACAAAGT GCTCAGTTCT GGAATGCCAC ATTTGCTAAA 
GTGACGATGT TGATTTATCC TGAGGAGTTA AAGCCAATAC TGAAACAAGC CAAACAAAAA 
TCTCTGCTTT TATTGCCTGG TTTGGAAAAT GTTGAAATTA TGGAGGAATC CAGTGGACCA 
TATTCAGATA TGACAGAAAA TTCCCAATTA AATATGAAGA TAAGTGGCAT GGAAAGAAAA 
TCAAGTGGAA AAAGAGATTC TTTTTTGGCA CAAACAAAGG ATACAAAAGA CAGTATGAAA 
CCACCAGCTA AATTGAAACT AGATTCTTCA ACTCCAAAAG CAAAGGGTGA AATGCCTTTG 
GAAGAAGAAA AGTCTACTGA CTTTGTGTTT ATACCTCCAG AAGTAAAAGA AACAAAGGAA 
AGAATACTAA CTGAACACCA GAAAGAAGTA CTCAAAACAA AGAGGTGTGA TATTCCTGCC 
ATGTATAATA ATCTGGATGT TTCACAAGAT ACTTTATTTT CTCAGTATAG TCAGGAAGAA 
TCTATGGAAA TTTCTACTTT AATTGAAAAA TCAAAAGAAG ACTCCAAGAT GATATTTAAG 
GAGGAACAAA AGGAGAATGA CATTGTCGTT CCTCAACATG TCATGGAAAA CTGCGGTATG 
GATGAACATC TTGAAAAGGC TTCCCTTTTG AATAATGAGT GTGGTTCTAT TGAGGAAACC 
AGTCCAAAAA TACTGAGCAG TAATAATGAT GCCAGAAAAA AAGCTTTAAT TGCTTCAAAT 
AAACCAACAG AATGTGCATC TACTACAGAA AATCCATCTG GTGTCAGCAG TAGCTCGGTT 
TCTATTGCCA CCATTTCTGG AGCTCCCTTA CAGCCTACAA GTCGAAGGCA ATCCTTTATT 
ACTTTGGAAA AGTTTGATGG TTCAGAAAAT AGACCTTTTA GTCCATCACC CTTGAATAAT 
ATGTCATCAA CTGTAATAGT GAAAAGTAAC CAGGAAGGCA TGGGTAAAAC AGATAATCCA 
TCAAAAGCAA AGAAGAGAGA AGTGGCTCTT TCCAAATCTG ACTCTGAAAA AATAACACAT 
GGGACTAAGA GATCAAGCCG AAGGTCGAGT AAATCTGAAC CAATAGGGAG TAAAAAGTCT 
AAACCCTTAA TGAGATCTGA ACAAGAGAAA AGTACTCAAG AATCTGTTGA TGGCACAGTA 
GCCTTAGAAA ATAACCCACC TGGCTTGCTT AATCAGACAG AATGTGTGTT AGATAATCAG 
GTTCATCTCT CTGAATCTAC AGTGGAGTTT GAGGATACAA TGCTTGAATC AACAGTGGAG 
AATACTGTAT TAGAAAACAA TACTGTAGAA GAGAGAACCT TAGAAGTTAA CTTGGAATCC 
AAAGAAAACA CACCCCCAGC CATAATAACG GATCAGACAG TAAATGAGGA TAGTCATATT 
CAGGTAACTC CAAATCAGAA AACCCTTAGG CGATCTTTAA GGCGACGTTC AGAAACAGCA 
GAACCAACCA CTGATAGCCA AGATAAAGAA AATAATCATC AAAAAAGGGA AAGACGTAAA 
GAAGAAGAAA AGACTCCGCA GAAGAGTCCG TTACATGTAA AAGATGATGT TTTACCTAAG 
CAAAAATTGA TTTCTGAACA AAGTGTACAG GGAAACTTAG TTGACAAAGG AAATAATTTA 
CATGAGAAGG CTTTTGGGGA GACCAGTGCT AATGCTGAAA TTGATGAAAA TAGAAGAAAG 
CCAGATCTTG AGAATACTAA ATCTGAAGGA GATGGTGTGC CGGACATTGC AGATAAATCC 
TCTGAGAAAC CAGTAAGGGG ACGAACACGA TACCAAACAA GAAGAGCATC CCAGGGTTTA 
CTTTCCAGCA TTGAAAACTC AGAATCTGAT AGTTCTGAGG CAAAAGAAGA AGGTTCTAAA 
AAGAAAAGAT CTGGAAAATG GAAGAACAAA AGCAGTGACA ATGTTGACAT GGAAGATCAA 
GAAGAGAAAA TGGTAAAACA GGAATGTATA AAAATTGAAA ATCAGGCACA TGATTATAAA 
GGGAATCCTG AAGTAGACAA AGATATAAAA TCTCAAATCT GTGAAAAAGG AGATGAAAGT 
ACTACTGTAA GTCTTCAAGA TTCTACCGTA TCTCCAGATT TATTACAAGT TTATGATGAT 
GTATTGAATA CTTCTGAGGG AGAAAGTAAA AGTAACAAAG ATGCAGACCA TTCCTTTTCA 
AATCCTTCTG TACCAGAATC AAATCTAAGG ACTAGAAATG CCAGTAAGAG GTTACATAAG 
CGAGATTCTA TAGAAAATAG CATGGGGGAA TCCTCAAAAA TAGGGACATC AGACATTTCT 
TTGCTTTCTG AAAAAACTCT TCAGACAGTA GAATGCCAGC ACAAGAGAAG TAGGAGGGTG 
AGGCGATCTA AAGGTTGTGA TTGTTGTGGA GAAAAATCAC AGTCTCAGGA AAAGTCATTT 
ACTGGGTTAA AGGATACAGA AAATTACGAT GTAAAAGTCA GTGAAACAAA AAAGACAGAT 
GGGGAAACAC CTGTATCTAT TTCAGAAACT TCGAAAGCTG ATCTGTCCTC GGAGGTTAAA 
CTTTTAGATG AGCATCATAG TGTGAATTTT CATTTGGATC TCAAGGAAGA GAATGATACT 
ACTAATGATT CATTAATTAT ATCTGAAACT GAGTTAAAAG AAAATACTAC TCAAAGCTTT 
CTTCCTTCTG AAATGGTAAA ATTTAAAGAA ACTTCTCGTA GGGATTCTGA GGAAGCAATA 
TCTCTAGAAA CCCAGGAGCC ATCTAATAAG AAATGTAAAA CTGTTGACCC ACATTTAGAT 
GTTTCCAAAG ATATTTCACT GAAATGTTTT TCTTTGGAGA CTAAAGAAGA AAAGTCAGAG 
GCCACTTCAA AAGAGAAATT GAGTACAGAG AACATTGTTA TTGTTGAATC AAATGCAGAA 
TCAAATCCAG AAGTAGATGC TATGGAATTG AATGTAGAAA ATGATCAGTC TGAAGCTGGC 
TTCTCTGAAC AAAAGAATGT CAAAACTGGT ATTCCAGGGA AAGAAGTAAC AGAGGAAAAC 
AGTGAAAGAA TGGATACACC TGAAAAACTG AAAGCCAATG AAGCAGTAAC TGAGGAATTT 
AATTCAGCTA CTGATTATTC GGATACTCCG ACATCTGTAA AAGTGAATGC TCAGGTAGAG 
ATCTCTGAAC AAATAGCGGT TGGGGAGCTA GGTGGAGGAA GTGATGAATC TGATCCAGGC 
TCATCTGAAG AAATGAACGC TGAAATGAAA AATTGTGAAA AAACAGCGGT TTGTGAGGTG 
ACTACTGAAT CTGACCACGT CACTGAAACC GAAGACGAAG ATGTAACTAC CACGGCTTCT 
CTGCCTATAG AAGTTGAAAC AAAGGAATTA GATGTAGAAG TCAATAACTT TGTTCCCGAC 
ACACTTGAGA TGATCACTGA AGAAGGAAAG GTTGACCCTA ACAAAACTGA AACGAATACT 
GAACCTAATA AATTGGAGGA AACACAGTTA GATGACAATC AGATGGTAGT GGAAAGTAAT 
GAAACTTTAC CGGAAGTTCA CCATACTTCA GAGAAAGTAG AGGAGATAGC ACAACCTCTG 
ACATCTGAAA CAGATATTTC TGGACTAATA TCAGAAGACA GTAATACATC TCCTCAAAAA 
TCGAAGGATA TTGATCCTTT ACTCATGTCA GCAAACGAGA GTCCTAGTGG CATGCACACA 
CGTTGTGTGT GGTCTCCTTT GGCTTCTCCA TCCACCAGCA TTTTAAAGAG AGGACTAAAA 
AGACCCCAAG AAGATGAAAT CTCGTCACCT GTCCACAAGC ATAATACCAC TTCAGCCAAA 
GGATTTCTGT CCCCAGGATC ACGTAGCTCT AAATTTAAGA GCTCAAAGAA GTGTTTAATT 
GCAGAAATGG CTAAGGAATC TGTGCCATGC CCAACAGAAA GTGTTTACCC TGCTTTGGTG 
AACTGTGTAG CACCAGTTGA AATCATTTTA CCTCAGATTA CATCCAACAT GTGGGCAAGA 
GGACTGGGAC AGCTCATTAG AGCCAAGAAT ATAAAAACAA TTGGCGATTT GAGTACCCTT 
ACAGCATCTG AAATAAAAAC TCTTCCTATC CGTTCTCCAA AAGTGTCTAA CGTTAAAAAG 
GCTCTCAGAA TATATCATGA GCAGCAGGTG AAGTCTCGTG GACTAGAAGA GATTCCAGTT 
TTTGATATTT CTGAAAAAAC AATAAATGGA ATGGAAAATA AATCTATCTC TCCTGATGAA 
GAAAGACTTG CCTCAGATTT AATTGATCCT GTTGCTTTAG AAGCTCCGTT ATCTAAAAAT 
CTTGTGGCAC AGATTAGCGC ACTTGCTCTT CAACTAGATT CAGAAGATCT CTATAATTAT 
TCAGGAAGCC AGCTATTTGA AATGCACGAG AAACTTGGTA GCATGGCAAA CTCTATAATT 
AAAAATCTGC AGTCACGTTG GAGGTCACCA GCCCATGAAA ATTCTTTTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010824793.1
CDS	146..7396
Translation	MTAADQSPLAPLLETLEDPSASLGEQTDAYLTLTSRMTGEDGKEIITEIEKKLPQLFKVLKTHIASQNSELSSAALQALGFCLYNPKIASGLSEINIQELLSKLNDIVKSSDKNVRTRALWVISKQTFPTEVVGKVVSSIIDSLEIVFNRGEMLSAVVDYEALNVIIRLIEQAPVQMGEESIRWAKLVIPLLVHSAQKVHLRGATALEMGMPLLLQKQQEIASITEQLMTTKLLSELQKLFMSKNETYVLKLWPLFVKLLGKTLHRSGSFINSLLQLEELGFRSGAPMIKKIAFIAWKSLIDNFALNPEILCSAKRLKLLMQPLSSIHVRTETLALTKLEVWWYLLMRLGPHLPANFEQVCVPLIQSTISIDSNASPQGSSSRVATTPGLSPMTPVHKGASPYGTPVTPRMNLNSNFGMATIPSIQLLGLEMLLHFLLGPEVVSFAKQNKLILSLEPLEHPLISSSSFFSKHSNTLITAVHDSFVAVGKDASDVVINAIWKELISLVKSVIESGNKKERPGSEVLTLLLKSLESIVNSEVFPVLKTLVLMEITIKGLPQKVLGSPAYQVANMDILNGTPALFLIQLTFNNNLLECGVEDERFFLNLETLVGCVLSGPTSPLAFSDSVLNVVNQNAKQLDNKEHLWRMWSIIVTPLTELINQTNEVNQGDALEHNFSAIYGALILPINHIFPAQKIPVVTMKTLLKTWSELYRAFARCAALVATAEENLCCEEFCSKIMSSLEDEGLSNLLFLDRITHIITVMVDCIDFSPYNIKYQPKTKSPQRPTDWSKKKKEPLGKLASLIKLIVRVIYSFHTLSLKEVHSDTLLTIGNSITSILSNLLGHISLPSMIQKIFAALTRPLALFYENSKLDEVPKVYSCMNNKLEKLLGDIIACLHVSYTGTYDSELLEQISPLLCIIFSHKNKQIRKQSAQFWNATFAKVTMLIYPEELKPILKQAKQKSLLLLPGLENVEIMEESSGPYSDMTENSQLNMKISGMERKSSGKRDSFLAQTKDTKDSMKPPAKLKLDSSTPKAKGEMPLEEEKSTDFVFIPPEVKETKERILTEHQKEVLKTKRCDIPAMYNNLDVSQDTLFSQYSQEESMEISTLIEKSKEDSKMIFKEEQKENDIVVPQHVMENCGMDEHLEKASLLNNECGSIEETSPKILSSNNDARKKALIASNKPTECASTTENPSGVSSSSVSIATISGAPLQPTSRRQSFITLEKFDGSENRPFSPSPLNNMSSTVIVKSNQEGMGKTDNPSKAKKREVALSKSDSEKITHGTKRSSRRSSKSEPIGSKKSKPLMRSEQEKSTQESVDGTVALENNPPGLLNQTECVLDNQVHLSESTVEFEDTMLESTVENTVLENNTVEERTLEVNLESKENTPPAIITDQTVNEDSHIQVTPNQKTLRRSLRRRSETAEPTTDSQDKENNHQKRERRKEEEKTPQKSPLHVKDDVLPKQKLISEQSVQGNLVDKGNNLHEKAFGETSANAEIDENRRKPDLENTKSEGDGVPDIADKSSEKPVRGRTRYQTRRASQGLLSSIENSESDSSEAKEEGSKKKRSGKWKNKSSDNVDMEDQEEKMVKQECIKIENQAHDYKGNPEVDKDIKSQICEKGDESTTVSLQDSTVSPDLLQVYDDVLNTSEGESKSNKDADHSFSNPSVPESNLRTRNASKRLHKRDSIENSMGESSKIGTSDISLLSEKTLQTVECQHKRSRRVRRSKGCDCCGEKSQSQEKSFTGLKDTENYDVKVSETKKTDGETPVSISETSKADLSSEVKLLDEHHSVNFHLDLKEENDTTNDSLIISETELKENTTQSFLPSEMVKFKETSRRDSEEAISLETQEPSNKKCKTVDPHLDVSKDISLKCFSLETKEEKSEATSKEKLSTENIVIVESNAESNPEVDAMELNVENDQSEAGFSEQKNVKTGIPGKEVTEENSERMDTPEKLKANEAVTEEFNSATDYSDTPTSVKVNAQVEISEQIAVGELGGGSDESDPGSSEEMNAEMKNCEKTAVCEVTTESDHVTETEDEDVTTTASLPIEVETKELDVEVNNFVPDTLEMITEEGKVDPNKTETNTEPNKLEETQLDDNQMVVESNETLPEVHHTSEKVEEIAQPLTSETDISGLISEDSNTSPQKSKDIDPLLMSANESPSGMHTRCVWSPLASPSTSILKRGLKRPQEDEISSPVHKHNTTSAKGFLSPGSRSSKFKSSKKCLIAEMAKESVPCPTESVYPALVNCVAPVEIILPQITSNMWARGLGQLIRAKNIKTIGDLSTLTASEIKTLPIRSPKVSNVKKALRIYHEQQVKSRGLEEIPVFDISEKTINGMENKSISPDEERLASDLIDPVALEAPLSKNLVAQISALALQLDSEDLYNYSGSQLFEMHEKLGSMANSIIKNLQSRWRSPAHENSF

Target ORF information:

RefSeq Version	XM_010826491.2
Organism	Bos taurus(cattle)
Definition	Bos taurus replication timing regulatory factor 1 (RIF1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010826491.2

ORF Insert Sequence:

ATGACGGCCG CGGATCAGAG CCCCCTGGCA CCGCTGTTGG AGACTTTGGA AGACCCTTCC 
GCCTCCCTTG GAGAGCAGAC CGACGCTTAC CTGACCCTGA CCAGTCGTAT GACTGGAGAA 
GATGGAAAAG AAATCATTAC AGAAATTGAG AAAAAACTTC CTCAGCTATT TAAAGTTTTA 
AAGACTCACA TTGCCAGTCA AAACTCAGAG CTCAGTAGTG CTGCTCTACA GGCCTTGGGG 
TTTTGCTTAT ATAATCCCAA AATTGCCTCT GGATTATCAG AGATAAATAT TCAAGAACTG 
CTTTCAAAAC TGAATGATAT TGTTAAAAGT TCAGATAAAA ATGTACGTAC TAGGGCACTT 
TGGGTGATAT CCAAGCAGAC GTTTCCCACT GAAGTCGTTG GCAAAGTAGT TTCTAGTATC 
ATTGACTCAC TAGAAATAGT ATTTAATAGA GGAGAGATGC TTTCTGCTGT TGTGGATTAT 
GAAGCATTAA ATGTCATTAT AAGACTAATT GAACAAGCCC CAGTTCAAAT GGGAGAAGAG 
TCAATTAGGT GGGCAAAATT GGTCATACCT TTACTGGTTC ATTCAGCCCA AAAAGTACAT 
TTGCGGGGAG CCACTGCTCT AGAGATGGGA ATGCCTTTGT TGCTTCAGAA ACAGCAAGAA 
ATAGCATCTA TCACAGAGCA GCTTATGACT ACCAAATTAC TTTCAGAACT CCAGAAACTG 
TTTATGAGTA AAAATGAGAC TTATGTGTTA AAATTGTGGC CTTTATTTGT CAAACTTCTT 
GGGAAGACCT TGCATCGAAG TGGGAGTTTC ATTAATTCTC TGTTACAGCT GGAAGAACTG 
GGATTTCGTA GTGGAGCACC CATGATTAAA AAAATAGCTT TTATTGCTTG GAAGAGTTTA 
ATAGATAATT TTGCTTTAAA TCCAGAAATA CTGTGTAGTG CAAAAAGACT GAAGTTGTTG 
ATGCAGCCTT TGAGTTCTAT CCATGTGAGA ACAGAAACTC TAGCGCTGAC AAAACTAGAA 
GTCTGGTGGT ATTTGTTGAT GAGACTAGGA CCTCATCTTC CTGCTAATTT TGAACAGGTT 
TGTGTGCCTC TGATTCAGAG CACAATAAGC ATTGATTCTA ATGCTTCACC TCAAGGGAGT 
TCATCTCGTG TAGCTACTAC TCCAGGTTTA AGCCCTATGA CCCCAGTACA CAAAGGTGCT 
TCTCCATATG GAACTCCTGT AACCCCTCGG ATGAACTTGA ATTCAAATTT TGGAATGGCC 
ACAATCCCCT CTATACAACT TTTGGGGCTT GAAATGTTGC TTCATTTTTT GTTGGGTCCA 
GAAGTCGTGA GTTTTGCTAA GCAAAACAAA CTTATACTGA GCCTAGAACC ACTTGAACAT 
CCATTAATCA GCAGCTCTTC TTTTTTCTCC AAACACTCAA ATACACTTAT CACTGCTGTT 
CATGATAGCT TTGTTGCAGT TGGAAAAGAT GCTTCTGATG TAGTTATTAA TGCTATTTGG 
AAGGAGTTAA TTAGCTTGGT GAAGTCAGTT ATTGAATCAG GTAACAAAAA AGAGAGACCA 
GGTTCTGAAG TTTTGACTCT CTTATTAAAA TCTTTGGAAA GCATAGTGAA TTCAGAAGTA 
TTTCCTGTAT TGAAAACACT GGTTCTCATG GAAATTACAA TTAAAGGACT TCCTCAGAAA 
GTATTAGGTT CTCCAGCATA TCAGGTTGCT AATATGGATA TTCTTAATGG AACCCCTGCT 
TTGTTCTTAA TTCAGTTAAC TTTCAATAAT AATCTATTGG AATGTGGTGT GGAAGACGAA 
AGGTTTTTTC TCAACCTGGA AACACTTGTG GGCTGTGTTC TTTCGGGTCC AACTTCACCA 
CTCGCTTTCA GTGACTCTGT CTTAAATGTT GTCAATCAAA ATGCAAAGCA GTTGGATAAC 
AAGGAGCATC TTTGGAGAAT GTGGAGTATT ATAGTCACCC CATTAACAGA ATTGATTAAT 
CAGACCAATG AAGTGAATCA AGGGGATGCC TTAGAACATA ATTTCAGTGC TATCTATGGT 
GCACTGATTT TGCCAATAAA CCATATTTTT CCAGCACAAA AAATTCCAGT GGTCACCATG 
AAGACCTTAC TTAAAACTTG GTCAGAATTA TATAGAGCAT TTGCTCGCTG TGCAGCTTTG 
GTGGCAACTG CGGAAGAGAA TTTATGCTGT GAGGAATTTT GTTCCAAGAT AATGTCCAGT 
TTGGAAGATG AAGGCCTTTC AAATTTATTG TTCTTGGATA GGATCACTCA TATTATTACT 
GTAATGGTTG ATTGCATCGA CTTTTCACCA TATAATATTA AATATCAACC CAAAACTAAA 
TCACCACAGA GACCTACAGA TTGGTCCAAA AAGAAGAAGG AACCTTTAGG GAAGTTGGCT 
TCTTTAATTA AACTTATTGT GAGAGTGATC TATTCTTTTC ACACACTGAG CCTCAAGGAA 
GTACATTCTG ATACTCTCCT CACTATTGGC AACTCAATCA CCAGCATTCT TTCCAATTTA 
CTTGGACATA TTTCTCTGCC TTCTATGATC CAGAAAATAT TTGCAGCTTT AACAAGACCT 
CTGGCATTAT TTTATGAAAA CTCAAAGCTT GATGAAGTTC CTAAAGTGTA TAGTTGTATG 
AACAACAAGT TAGAAAAGCT ACTAGGAGAT ATAATTGCTT GTCTACATGT CAGCTACACT 
GGAACTTACG ACAGTGAACT TCTTGAACAG ATCTCACCAT TATTATGCAT AATATTTTCA 
CACAAGAACA AACAGATAAG AAAACAAAGT GCTCAGTTCT GGAATGCCAC ATTTGCTAAA 
GTGACGATGT TGATTTATCC TGAGGAGTTA AAGCCAATAC TGAAACAAGC CAAACAAAAA 
TCTCTGCTTT TATTGCCTGG TTTGGAAAAT GTTGAAATTA TGGAGGAATC CAGTGGACCA 
TATTCAGATA TGACAGAAAA TTCCCAATTA AATATGAAGA TAAGTGGCAT GGAAAGAAAA 
TCAAGTGGAA AAAGAGATTC TTTTTTGGCA CAAACAAAGG ATACAAAAGA CAGTATGAAA 
CCACCAGCTA AATTGAAACT AGATTCTTCA ACTCCAAAAG CAAAGGGTGA AATGCCTTTG 
GAAGAAGAAA AGTCTACTGA CTTTGTGTTT ATACCTCCAG AAGTAAAAGA AACAAAGGAA 
AGAATACTAA CTGAACACCA GAAAGAAGTA CTCAAAACAA AGAGGTGTGA TATTCCTGCC 
ATGTATAATA ATCTGGATGT TTCACAAGAT ACTTTATTTT CTCAGTATAG TCAGGAAGAA 
TCTATGGAAA TTTCTACTTT AATTGAAAAA TCAAAAGAAG ACTCCAAGAT GATATTTAAG 
GAGGAACAAA AGGAGAATGA CATTGTCGTT CCTCAACATG TCATGGAAAA CTGCGGTATG 
GATGAACATC TTGAAAAGGC TTCCCTTTTG AATAATGAGT GTGGTTCTAT TGAGGAAACC 
AGTCCAAAAA TACTGAGCAG TAATAATGAT GCCAGAAAAA AAGCTTTAAT TGCTTCAAAT 
AAACCAACAG AATGTGCATC TACTACAGAA AATCCATCTG GTGTCAGCAG TAGCTCGGTT 
TCTATTGCCA CCATTTCTGG AGCTCCCTTA CAGCCTACAA GTCGAAGGCA ATCCTTTATT 
ACTTTGGAAA AGTTTGATGG TTCAGAAAAT AGACCTTTTA GTCCATCACC CTTGAATAAT 
ATGTCATCAA CTGTAATAGT GAAAAGTAAC CAGGAAGGCA TGGGTAAAAC AGATAATCCA 
TCAAAAGCAA AGAAGAGAGA AGTGGCTCTT TCCAAATCTG ACTCTGAAAA AATAACACAT 
GGGACTAAGA GATCAAGCCG AAGGTCGAGT AAATCTGAAC CAATAGGGAG TAAAAAGTCT 
AAACCCTTAA TGAGATCTGA ACAAGAGAAA AGTACTCAAG AATCTGTTGA TGGCACAGTA 
GCCTTAGAAA ATAACCCACC TGGCTTGCTT AATCAGACAG AATGTGTGTT AGATAATCAG 
GTTCATCTCT CTGAATCTAC AGTGGAGTTT GAGGATACAA TGCTTGAATC AACAGTGGAG 
AATACTGTAT TAGAAAACAA TACTGTAGAA GAGAGAACCT TAGAAGTTAA CTTGGAATCC 
AAAGAAAACA CACCCCCAGC CATAATAACG GATCAGACAG TAAATGAGGA TAGTCATATT 
CAGGTAACTC CAAATCAGAA AACCCTTAGG CGATCTTTAA GGCGACGTTC AGAAACAGCA 
GAACCAACCA CTGATAGCCA AGATAAAGAA AATAATCATC AAAAAAGGGA AAGACGTAAA 
GAAGAAGAAA AGACTCCGCA GAAGAGTCCG TTACATGTAA AAGATGATGT TTTACCTAAG 
CAAAAATTGA TTTCTGAACA AAGTGTACAG GGAAACTTAG TTGACAAAGG AAATAATTTA 
CATGAGAAGG CTTTTGGGGA GACCAGTGCT AATGCTGAAA TTGATGAAAA TAGAAGAAAG 
CCAGATCTTG AGAATACTAA ATCTGAAGGA GATGGTGTGC CGGACATTGC AGATAAATCC 
TCTGAGAAAC CAGTAAGGGG ACGAACACGA TACCAAACAA GAAGAGCATC CCAGGGTTTA 
CTTTCCAGCA TTGAAAACTC AGAATCTGAT AGTTCTGAGG CAAAAGAAGA AGGTTCTAAA 
AAGAAAAGAT CTGGAAAATG GAAGAACAAA AGCAGTGACA ATGTTGACAT GGAAGATCAA 
GAAGAGAAAA TGGTAAAACA GGAATGTATA AAAATTGAAA ATCAGGCACA TGATTATAAA 
GGGAATCCTG AAGTAGACAA AGATATAAAA TCTCAAATCT GTGAAAAAGG AGATGAAAGT 
ACTACTGTAA GTCTTCAAGA TTCTACCGTA TCTCCAGATT TATTACAAGT TTATGATGAT 
GTATTGAATA CTTCTGAGGG AGAAAGTAAA AGTAACAAAG ATGCAGACCA TTCCTTTTCA 
AATCCTTCTG TACCAGAATC AAATCTAAGG ACTAGAAATG CCAGTAAGAG GTTACATAAG 
CGAGATTCTA TAGAAAATAG CATGGGGGAA TCCTCAAAAA TAGGGACATC AGACATTTCT 
TTGCTTTCTG AAAAAACTCT TCAGACAGTA GAATGCCAGC ACAAGAGAAG TAGGAGGGTG 
AGGCGATCTA AAGGTTGTGA TTGTTGTGGA GAAAAATCAC AGTCTCAGGA AAAGTCATTT 
ACTGGGTTAA AGGATACAGA AAATTACGAT GTAAAAGTCA GTGAAACAAA AAAGACAGAT 
GGGGAAACAC CTGTATCTAT TTCAGAAACT TCGAAAGCTG ATCTGTCCTC GGAGGTTAAA 
CTTTTAGATG AGCATCATAG TGTGAATTTT CATTTGGATC TCAAGGAAGA GAATGATACT 
ACTAATGATT CATTAATTAT ATCTGAAACT GAGTTAAAAG AAAATACTAC TCAAAGCTTT 
CTTCCTTCTG AAATGGTAAA ATTTAAAGAA ACTTCTCGTA GGGATTCTGA GGAAGCAATA 
TCTCTAGAAA CCCAGGAGCC ATCTAATAAG AAATGTAAAA CTGTTGACCC ACATTTAGAT 
GTTTCCAAAG ATATTTCACT GAAATGTTTT TCTTTGGAGA CTAAAGAAGA AAAGTCAGAG 
GCCACTTCAA AAGAGAAATT GAGTACAGAG AACATTGTTA TTGTTGAATC AAATGCAGAA 
TCAAATCCAG AAGTAGATGC TATGGAATTG AATGTAGAAA ATGATCAGTC TGAAGCTGGC 
TTCTCTGAAC AAAAGAATGT CAAAACTGGT ATTCCAGGGA AAGAAGTAAC AGAGGAAAAC 
AGTGAAAGAA TGGATACACC TGAAAAACTG AAAGCCAATG AAGCAGTAAC TGAGGAATTT 
AATTCAGCTA CTGATTATTC GGATACTCCG ACATCTGTAA AAGTGAATGC TCAGGTAGAG 
ATCTCTGAAC AAATAGCGGT TGGGGAGCTA GGTGGAGGAA GTGATGAATC TGATCCAGGC 
TCATCTGAAG AAATGAACGC TGAAATGAAA AATTGTGAAA AAACAGCGGT TTGTGAGGTG 
ACTACTGAAT CTGACCACGT CACTGAAACC GAAGACGAAG ATGTAACTAC CACGGCTTCT 
CTGCCTATAG AAGTTGAAAC AAAGGAATTA GATGTAGAAG TCAATAACTT TGTTCCCGAC 
ACACTTGAGA TGATCACTGA AGAAGGAAAG GTTGACCCTA ACAAAACTGA AACGAATACT 
GAACCTAATA AATTGGAGGA AACACAGTTA GATGACAATC AGATGGTAGT GGAAAGTAAT 
GAAACTTTAC CGGAAGTTCA CCATACTTCA GAGAAAGTAG AGGAGATAGC ACAACCTCTG 
ACATCTGAAA CAGATATTTC TGGACTAATA TCAGAAGACA GTAATACATC TCCTCAAAAA 
TCGAAGGATA TTGATCCTTT ACTCATGTCA GCAAACGAGA GTCCTAGTGG CATGCACACA 
CGTTGTGTGT GGTCTCCTTT GGCTTCTCCA TCCACCAGCA TTTTAAAGAG AGGACTAAAA 
AGACCCCAAG AAGATGAAAT CTCGTCACCT GTCCACAAGC ATAATACCAC TTCAGCCAAA 
GGATTTCTGT CCCCAGGATC ACGTAGCTCT AAATTTAAGA GCTCAAAGAA GTGTTTAATT 
GCAGAAATGG CTAAGGAATC TGTGCCATGC CCAACAGAAA GTGTTTACCC TGCTTTGGTG 
AACTGTGTAG CACCAGTTGA AATCATTTTA CCTCAGATTA CATCCAACAT GTGGGCAAGA 
GGACTGGGAC AGCTCATTAG AGCCAAGAAT ATAAAAACAA TTGGCGATTT GAGTACCCTT 
ACAGCATCTG AAATAAAAAC TCTTCCTATC CGTTCTCCAA AAGTGTCTAA CGTTAAAAAG 
GCTCTCAGAA TATATCATGA GCAGCAGGTG AAGTCTCGTG GACTAGAAGA GATTCCAGTT 
TTTGATATTT CTGAAAAAAC AATAAATGGA ATGGAAAATA AATCTATCTC TCCTGATGAA 
GAAAGACTTG CCTCAGATTT AATTGATCCT GTTGCTTTAG AAGCTCCGTT ATCTAAAAAT 
CTTGTGGCAC AGATTAGCGC ACTTGCTCTT CAACTAGATT CAGAAGATCT CTATAATTAT 
TCAGGAAGCC AGCTATTTGA AATGCACGAG AAACTTGGTA GCATGGCAAA CTCTATAATT 
AAAAATCTGC AGTCACGTTG GAGGTCACCA GCCCATGAAA ATTCTTTTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RIF1 ( XM_010826493.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010824795.1 Bos taurus replication timing regulatory factor 1 (RIF1), transcript variant X6, mRNA.

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CloneID	OBa55068
Clone ID Related Accession (Same CDS sequence)	XM_010826493.2 , XM_010802077.3 , XM_010802077.2
Accession Version	XM_010826493.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7173bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	telomere-associated protein RIF1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644591.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010826493.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGCCG CGGATCAGAG CCCCCTGGCA CCGCTGTTGG AGACTTTGGA AGACCCTTCC 
GCCTCCCTTG GAGAGCAGAC CGACGCTTAC CTGACCCTGA CCAGTCGTAT GACTGGAGAA 
GATGGAAAAG AAATCATTAC AGAAATTGAG AAAAAACTTC CTCAGCTATT TAAAGTTTTA 
AAGACTCACA TTGCCAGTCA AAACTCAGAG CTCAGTAGTG CTGCTCTACA GGCCTTGGGG 
TTTTGCTTAT ATAATCCCAA AATTGCCTCT GGATTATCAG AGATAAATAT TCAAGAACTG 
CTTTCAAAAC TGAATGATAT TGTTAAAAGT TCAGATAAAA ATGTACGTAC TAGGGCACTT 
TGGGTGATAT CCAAGCAGAC GTTTCCCACT GAAGTCGTTG GCAAAGTAGT TTCTAGTATC 
ATTGACTCAC TAGAAATAGT ATTTAATAGA GGAGAGATGC TTTCTGCTGT TGTGGATTAT 
GAAGCATTAA ATGTCATTAT AAGACTAATT GAACAAGCCC CAGTTCAAAT GGGAGAAGAG 
TCAATTAGGT GGGCAAAATT GGTCATACCT TTACTGGTTC ATTCAGCCCA AAAAGTACAT 
TTGCGGGGAG CCACTGCTCT AGAGATGGGA ATGCCTTTGT TGCTTCAGAA ACAGCAAGAA 
ATAGCATCTA TCACAGAGCA GCTTATGACT ACCAAATTAC TTTCAGAACT CCAGAAACTG 
TTTATGAGTA AAAATGAGAC TTATGTGTTA AAATTGTGGC CTTTATTTGT CAAACTTCTT 
GGGAAGACCT TGCATCGAAG TGGGAGTTTC ATTAATTCTC TGTTACAGCT GGAAGAACTG 
GGATTTCGTA GTGGAGCACC CATGATTAAA AAAATAGCTT TTATTGCTTG GAAGAGTTTA 
ATAGATAATT TTGCTTTAAA TCCAGAAATA CTGTGTAGTG CAAAAAGACT GAAGTTGTTG 
ATGCAGCCTT TGAGTTCTAT CCATGTGAGA ACAGAAACTC TAGCGCTGAC AAAACTAGAA 
GTCTGGTGGT ATTTGTTGAT GAGACTAGGA CCTCATCTTC CTGCTAATTT TGAACAGGTT 
TGTGTGCCTC TGATTCAGAG CACAATAAGC ATTGATTCTA ATGCTTCACC TCAAGGGAGT 
TCATCTCGTG TAGCTACTAC TCCAGGTTTA AGCCCTATGA CCCCAGTACA CAAAGGTGCT 
TCTCCATATG GAACTCCTGT AACCCCTCGG ATGAACTTGA ATTCAAATTT TGGAATGGCC 
ACAATCCCCT CTATACAACT TTTGGGGCTT GAAATGTTGC TTCATTTTTT GTTGGGTCCA 
GAAGTCGTGA GTTTTGCTAA GCAAAACAAA CTTATACTGA GCCTAGAACC ACTTGAACAT 
CCATTAATCA GCAGCTCTTC TTTTTTCTCC AAACACTCAA ATACACTTAT CACTGCTGTT 
CATGATAGCT TTGTTGCAGT TGGAAAAGAT GCTTCTGATG TAGTTATTAA TGCTATTTGG 
AAGGAGTTAA TTAGCTTGGT GAAGTCAGTT ATTGAATCAG GTAACAAAAA AGAGAGACCA 
GGTTCTGAAG TTTTGACTCT CTTATTAAAA TCTTTGGAAA GCATAGTGAA TTCAGAAGTA 
TTTCCTGTAT TGAAAACACT GGTTCTCATG GAAATTACAA TTAAAGGACT TCCTCAGAAA 
GTATTAGGTT CTCCAGCATA TCAGGTTGCT AATATGGATA TTCTTAATGG AACCCCTGCT 
TTGTTCTTAA TTCAGTTAAC TTTCAATAAT AATCTATTGG AATGTGGTGT GGAAGACGAA 
AGGTTTTTTC TCAACCTGGA AACACTTGTG GGCTGTGTTC TTTCGGGTCC AACTTCACCA 
CTCGCTTTCA GTGACTCTGT CTTAAATGTT GTCAATCAAA ATGCAAAGCA GTTGGATAAC 
AAGGAGCATC TTTGGAGAAT GTGGAGTATT ATAGTCACCC CATTAACAGA ATTGATTAAT 
CAGACCAATG AAGTGAATCA AGGGGATGCC TTAGAACATA ATTTCAGTGC TATCTATGGT 
GCACTGATTT TGCCAATAAA CCATATTTTT CCAGCACAAA AAATTCCAGT GGTCACCATG 
AAGACCTTAC TTAAAACTTG GTCAGAATTA TATAGAGCAT TTGCTCGCTG TGCAGCTTTG 
GTGGCAACTG CGGAAGAGAA TTTATGCTGT GAGGAATTTT GTTCCAAGAT AATGTCCAGT 
TTGGAAGATG AAGGCCTTTC AAATTTATTG TTCTTGGATA GGATCACTCA TATTATTACT 
GTAATGGTTG ATTGCATCGA CTTTTCACCA TATAATATTA AATATCAACC CAAAACTAAA 
TCACCACAGA GACCTACAGA TTGGTCCAAA AAGAAGAAGG AACCTTTAGG GAAGTTGGCT 
TCTTTAATTA AACTTATTGT GAGAGTGATC TATTCTTTTC ACACACTGAG CCTCAAGGAA 
GTACATTCTG ATACTCTCCT CACTATTGGC AACTCAATCA CCAGCATTCT TTCCAATTTA 
CTTGGACATA TTTCTCTGCC TTCTATGATC CAGAAAATAT TTGCAGCTTT AACAAGACCT 
CTGGCATTAT TTTATGAAAA CTCAAAGCTT GATGAAGTTC CTAAAGTGTA TAGTTGTATG 
AACAACAAGT TAGAAAAGCT ACTAGGAGAT ATAATTGCTT GTCTACATGT CAGCTACACT 
GGAACTTACG ACAGTGAACT TCTTGAACAG ATCTCACCAT TATTATGCAT AATATTTTCA 
CACAAGAACA AACAGATAAG AAAACAAAGT GCTCAGTTCT GGAATGCCAC ATTTGCTAAA 
GTGACGATGT TGATTTATCC TGAGGAGTTA AAGCCAATAC TGAAACAAGC CAAACAAAAA 
TCTCTGCTTT TATTGCCTGG TTTGGAAAAT GTTGAAATTA TGGAGGAATC CAGTGGACCA 
TATTCAGATA TGACAGAAAA TTCCCAATTA AATATGAAGA TAAGTGGCAT GGAAAGAAAA 
TCAAGTGGAA AAAGAGATTC TTTTTTGGCA CAAACAAAGG ATACAAAAGA CAGTATGAAA 
CCACCAGCTA AATTGAAACT AGATTCTTCA ACTCCAAAAG CAAAGGGTGA AATGCCTTTG 
GAAGAAGAAA AGTCTACTGA CTTTGTGTTT ATACCTCCAG AAGTAAAAGA AACAAAGGAA 
AGAATACTAA CTGAACACCA GAAAGAAGTA CTCAAAACAA AGAGGTGTGA TATTCCTGCC 
ATGTATAATA ATCTGGATGT TTCACAAGAT ACTTTATTTT CTCAGTATAG TCAGGAAGAA 
TCTATGGAAA TTTCTACTTT AATTGAAAAA TCAAAAGAAG ACTCCAAGAT GATATTTAAG 
GAGGAACAAA AGGAGAATGA CATTGTCGTT CCTCAACATG TCATGGAAAA CTGCGGTATG 
GATGAACATC TTGAAAAGGC TTCCCTTTTG AATAATGAGT GTGGTTCTAT TGAGGAAACC 
AGTCCAAAAA TACTGAGCAG TAATAATGAT GCCAGAAAAA AAGCTTTAAT TGCTTCAAAT 
AAACCAACAG AATGTGCATC TACTACAGAA AATCCATCTG GTGTCAGCAG TAGCTCGGTT 
TCTATTGCCA CCATTTCTGG AGCTCCCTTA CAGCCTACAA GTCGAAGGCA ATCCTTTATT 
ACTTTGGAAA AGTTTGATGG TTCAGAAAAT AGACCTTTTA GTCCATCACC CTTGAATAAT 
ATGTCATCAA CTGTAATAGT GAAAAGTAAC CAGGAAGGCA TGGGTAAAAC AGATAATCCA 
TCAAAAGCAA AGAAGAGAGA AGTGGCTCTT TCCAAATCTG ACTCTGAAAA AATAACACAT 
GGGACTAAGA GATCAAGCCG AAGGTCGAGT AAATCTGAAC CAATAGGGAG TAAAAAGTCT 
AAACCCTTAA TGAGATCTGA ACAAGAGAAA AGTACTCAAG AATCTGTTGA TGGCACAGTA 
GCCTTAGAAA ATAACCCACC TGGCTTGCTT AATCAGACAG AATGTGTGTT AGATAATCAG 
GTTCATCTCT CTGAATCTAC AGTGGAGTTT GAGGATACAA TGCTTGAATC AACAGTGGAG 
AATACTGTAT TAGAAAACAA TACTGTAGAA GAGAGAACCT TAGAAGTTAA CTTGGAATCC 
AAAGAAAACA CACCCCCAGC CATAATAACG GATCAGACAG TAAATGAGGA TAGTCATATT 
CAGGTAACTC CAAATCAGAA AACCCTTAGG CGATCTTTAA GGCGACGTTC AGAAACAGCA 
GAACCAACCA CTGATAGCCA AGATAAAGAA AATAATCATC AAAAAAGGGA AAGACGTAAA 
GAAGAAGAAA AGACTCCGCA GAAGAGTCCG TTACATGTAA AAGATGATGT TTTACCTAAG 
CAAAAATTGA TTTCTGAACA AAGTGTACAG GGAAACTTAG TTGACAAAGG AAATAATTTA 
CATGAGAAGG CTTTTGGGGA GACCAGTGCT AATGCTGAAA TTGATGAAAA TAGAAGAAAG 
CCAGATCTTG AGAATACTAA ATCTGAAGGA GATGGTGTGC CGGACATTGC AGATAAATCC 
TCTGAGAAAC CAGTAAGGGG ACGAACACGA TACCAAACAA GAAGAGCATC CCAGGGTTTA 
CTTTCCAGCA TTGAAAACTC AGAATCTGAT AGTTCTGAGG CAAAAGAAGA AGGTTCTAAA 
AAGAAAAGAT CTGGAAAATG GAAGAACAAA AGCAGTGACA ATGTTGACAT GGAAGATCAA 
GAAGAGAAAA TGGTAAAACA GGAATGTATA AAAATTGAAA ATCAGGCACA TGATTATAAA 
GGGAATCCTG AAGTAGACAA AGATATAAAA TCTCAAATCT GTGAAAAAGG AGATGAAAGT 
ACTACTGTAA GTCTTCAAGA TTCTACCGTA TCTCCAGATT TATTACAAGT TTATGATGAT 
GTATTGAATA CTTCTGAGGG AGAAAGTAAA AGTAACAAAG ATGCAGACCA TTCCTTTTCA 
AATCCTTCTG TACCAGAATC AAATCTAAGG ACTAGAAATG CCAGTAAGAG GTTACATAAG 
CGAGATTCTA TAGAAAATAG CATGGGGGAA TCCTCAAAAA TAGGGACATC AGACATTTCT 
TTGCTTTCTG AAAAAACTCT TCAGACAGTA GAATGCCAGC ACAAGAGAAG TAGGAGGGTG 
AGGCGATCTA AAGGTTGTGA TTGTTGTGGA GAAAAATCAC AGTCTCAGGA AAAGTCATTT 
ACTGGGTTAA AGGATACAGA AAATTACGAT GTAAAAGTCA GTGAAACAAA AAAGACAGAT 
GGGGAAACAC CTGTATCTAT TTCAGAAACT TCGAAAGCTG ATCTGTCCTC GGAGGTTAAA 
CTTTTAGATG AGCATCATAG TGTGAATTTT CATTTGGATC TCAAGGAAGA GAATGATACT 
ACTAATGATT CATTAATTAT ATCTGAAACT GAGTTAAAAG AAAATACTAC TCAAAGCTTT 
CTTCCTTCTG AAATGGTAAA ATTTAAAGAA ACTTCTCGTA GGGATTCTGA GGAAGCAATA 
TCTCTAGAAA CCCAGGAGCC ATCTAATAAG AAATGTAAAA CTGTTGACCC ACATTTAGAT 
GTTTCCAAAG ATATTTCACT GAAATGTTTT TCTTTGGAGA CTAAAGAAGA AAAGTCAGAG 
GCCACTTCAA AAGAGAAATT GAGTACAGAG AACATTGTTA TTGTTGAATC AAATGCAGAA 
TCAAATCCAG AAGTAGATGC TATGGAATTG AATGTAGAAA ATGATCAGTC TGAAGCTGGC 
TTCTCTGAAC AAAAGAATGT CAAAACTGGT ATTCCAGGGA AAGAAGTAAC AGAGGAAAAC 
AGTGAAAGAA TGGATACACC TGAAAAACTG AAAGCCAATG AAGCAGTAAC TGAGGAATTT 
AATTCAGCTA CTGATTATTC GGATACTCCG ACATCTGTAA AAGTGAATGC TCAGGTAGAG 
ATCTCTGAAC AAATAGCGGT TGGGGAGCTA GGTGGAGGAA GTGATGAATC TGATCCAGGC 
TCATCTGAAG AAATGAACGC TGAAATGAAA AATTGTGAAA AAACAGCGGT TTGTGAGGTG 
ACTACTGAAT CTGACCACGT CACTGAAACC GAAGACGAAG ATGTAACTAC CACGGCTTCT 
CTGCCTATAG AAGTTGAAAC AAAGGAATTA GATGTAGAAG TCAATAACTT TGTTCCCGAC 
ACACTTGAGA TGATCACTGA AGAAGGAAAG GTTGACCCTA ACAAAACTGA AACGAATACT 
GAACCTAATA AATTGGAGGA AACACAGTTA GATGACAATC AGATGGTAGT GGAAAGTAAT 
GAAACTTTAC CGGAAGTTCA CCATACTTCA GAGAAAGTAG AGGAGATAGC ACAACCTCTG 
ACATCTGAAA CAGATATTTC TGGACTAATA TCAGAAGACA GTAATACATC TCCTCAAAAA 
TCGAAGGATA TTGATCCTTT ACTCATGTCA GCAAACGAGA GTCCTAGTGG CATGCACACA 
CGTTGTGTGT GGTCTCCTTT GGCTTCTCCA TCCACCAGCA TTTTAAAGAG AGGACTAAAA 
AGACCCCAAG AAGATGAAAT CTCGTCACCT GTCCACAAGA TTGCAGAAAT GGCTAAGGAA 
TCTGTGCCAT GCCCAACAGA AAGTGTTTAC CCTGCTTTGG TGAACTGTGT AGCACCAGTT 
GAAATCATTT TACCTCAGAT TACATCCAAC ATGTGGGCAA GAGGACTGGG ACAGCTCATT 
AGAGCCAAGA ATATAAAAAC AATTGGCGAT TTGAGTACCC TTACAGCATC TGAAATAAAA 
ACTCTTCCTA TCCGTTCTCC AAAAGTGTCT AACGTTAAAA AGGCTCTCAG AATATATCAT 
GAGCAGCAGG TGAAGTCTCG TGGACTAGAA GAGATTCCAG TTTTTGATAT TTCTGAAAAA 
ACAATAAATG GAATGGAAAA TAAATCTATC TCTCCTGATG AAGAAAGACT TGCCTCAGAT 
TTAATTGATC CTGTTGCTTT AGAAGCTCCG TTATCTAAAA ATCTTGTGGC ACAGATTAGC 
GCACTTGCTC TTCAACTAGA TTCAGAAGAT CTCTATAATT ATTCAGGAAG CCAGCTATTT 
GAAATGCACG AGAAACTTGG TAGCATGGCA AACTCTATAA TTAAAAATCT GCAGTCACGT 
TGGAGGTCAC CAGCCCATGA AAATTCTTTT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010824795.1
CDS	146..7318
Translation	MTAADQSPLAPLLETLEDPSASLGEQTDAYLTLTSRMTGEDGKEIITEIEKKLPQLFKVLKTHIASQNSELSSAALQALGFCLYNPKIASGLSEINIQELLSKLNDIVKSSDKNVRTRALWVISKQTFPTEVVGKVVSSIIDSLEIVFNRGEMLSAVVDYEALNVIIRLIEQAPVQMGEESIRWAKLVIPLLVHSAQKVHLRGATALEMGMPLLLQKQQEIASITEQLMTTKLLSELQKLFMSKNETYVLKLWPLFVKLLGKTLHRSGSFINSLLQLEELGFRSGAPMIKKIAFIAWKSLIDNFALNPEILCSAKRLKLLMQPLSSIHVRTETLALTKLEVWWYLLMRLGPHLPANFEQVCVPLIQSTISIDSNASPQGSSSRVATTPGLSPMTPVHKGASPYGTPVTPRMNLNSNFGMATIPSIQLLGLEMLLHFLLGPEVVSFAKQNKLILSLEPLEHPLISSSSFFSKHSNTLITAVHDSFVAVGKDASDVVINAIWKELISLVKSVIESGNKKERPGSEVLTLLLKSLESIVNSEVFPVLKTLVLMEITIKGLPQKVLGSPAYQVANMDILNGTPALFLIQLTFNNNLLECGVEDERFFLNLETLVGCVLSGPTSPLAFSDSVLNVVNQNAKQLDNKEHLWRMWSIIVTPLTELINQTNEVNQGDALEHNFSAIYGALILPINHIFPAQKIPVVTMKTLLKTWSELYRAFARCAALVATAEENLCCEEFCSKIMSSLEDEGLSNLLFLDRITHIITVMVDCIDFSPYNIKYQPKTKSPQRPTDWSKKKKEPLGKLASLIKLIVRVIYSFHTLSLKEVHSDTLLTIGNSITSILSNLLGHISLPSMIQKIFAALTRPLALFYENSKLDEVPKVYSCMNNKLEKLLGDIIACLHVSYTGTYDSELLEQISPLLCIIFSHKNKQIRKQSAQFWNATFAKVTMLIYPEELKPILKQAKQKSLLLLPGLENVEIMEESSGPYSDMTENSQLNMKISGMERKSSGKRDSFLAQTKDTKDSMKPPAKLKLDSSTPKAKGEMPLEEEKSTDFVFIPPEVKETKERILTEHQKEVLKTKRCDIPAMYNNLDVSQDTLFSQYSQEESMEISTLIEKSKEDSKMIFKEEQKENDIVVPQHVMENCGMDEHLEKASLLNNECGSIEETSPKILSSNNDARKKALIASNKPTECASTTENPSGVSSSSVSIATISGAPLQPTSRRQSFITLEKFDGSENRPFSPSPLNNMSSTVIVKSNQEGMGKTDNPSKAKKREVALSKSDSEKITHGTKRSSRRSSKSEPIGSKKSKPLMRSEQEKSTQESVDGTVALENNPPGLLNQTECVLDNQVHLSESTVEFEDTMLESTVENTVLENNTVEERTLEVNLESKENTPPAIITDQTVNEDSHIQVTPNQKTLRRSLRRRSETAEPTTDSQDKENNHQKRERRKEEEKTPQKSPLHVKDDVLPKQKLISEQSVQGNLVDKGNNLHEKAFGETSANAEIDENRRKPDLENTKSEGDGVPDIADKSSEKPVRGRTRYQTRRASQGLLSSIENSESDSSEAKEEGSKKKRSGKWKNKSSDNVDMEDQEEKMVKQECIKIENQAHDYKGNPEVDKDIKSQICEKGDESTTVSLQDSTVSPDLLQVYDDVLNTSEGESKSNKDADHSFSNPSVPESNLRTRNASKRLHKRDSIENSMGESSKIGTSDISLLSEKTLQTVECQHKRSRRVRRSKGCDCCGEKSQSQEKSFTGLKDTENYDVKVSETKKTDGETPVSISETSKADLSSEVKLLDEHHSVNFHLDLKEENDTTNDSLIISETELKENTTQSFLPSEMVKFKETSRRDSEEAISLETQEPSNKKCKTVDPHLDVSKDISLKCFSLETKEEKSEATSKEKLSTENIVIVESNAESNPEVDAMELNVENDQSEAGFSEQKNVKTGIPGKEVTEENSERMDTPEKLKANEAVTEEFNSATDYSDTPTSVKVNAQVEISEQIAVGELGGGSDESDPGSSEEMNAEMKNCEKTAVCEVTTESDHVTETEDEDVTTTASLPIEVETKELDVEVNNFVPDTLEMITEEGKVDPNKTETNTEPNKLEETQLDDNQMVVESNETLPEVHHTSEKVEEIAQPLTSETDISGLISEDSNTSPQKSKDIDPLLMSANESPSGMHTRCVWSPLASPSTSILKRGLKRPQEDEISSPVHKIAEMAKESVPCPTESVYPALVNCVAPVEIILPQITSNMWARGLGQLIRAKNIKTIGDLSTLTASEIKTLPIRSPKVSNVKKALRIYHEQQVKSRGLEEIPVFDISEKTINGMENKSISPDEERLASDLIDPVALEAPLSKNLVAQISALALQLDSEDLYNYSGSQLFEMHEKLGSMANSIIKNLQSRWRSPAHENSF

Target ORF information:

RefSeq Version	XM_010826493.2
Organism	Bos taurus(cattle)
Definition	Bos taurus replication timing regulatory factor 1 (RIF1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010826493.2

ORF Insert Sequence:

ATGACGGCCG CGGATCAGAG CCCCCTGGCA CCGCTGTTGG AGACTTTGGA AGACCCTTCC 
GCCTCCCTTG GAGAGCAGAC CGACGCTTAC CTGACCCTGA CCAGTCGTAT GACTGGAGAA 
GATGGAAAAG AAATCATTAC AGAAATTGAG AAAAAACTTC CTCAGCTATT TAAAGTTTTA 
AAGACTCACA TTGCCAGTCA AAACTCAGAG CTCAGTAGTG CTGCTCTACA GGCCTTGGGG 
TTTTGCTTAT ATAATCCCAA AATTGCCTCT GGATTATCAG AGATAAATAT TCAAGAACTG 
CTTTCAAAAC TGAATGATAT TGTTAAAAGT TCAGATAAAA ATGTACGTAC TAGGGCACTT 
TGGGTGATAT CCAAGCAGAC GTTTCCCACT GAAGTCGTTG GCAAAGTAGT TTCTAGTATC 
ATTGACTCAC TAGAAATAGT ATTTAATAGA GGAGAGATGC TTTCTGCTGT TGTGGATTAT 
GAAGCATTAA ATGTCATTAT AAGACTAATT GAACAAGCCC CAGTTCAAAT GGGAGAAGAG 
TCAATTAGGT GGGCAAAATT GGTCATACCT TTACTGGTTC ATTCAGCCCA AAAAGTACAT 
TTGCGGGGAG CCACTGCTCT AGAGATGGGA ATGCCTTTGT TGCTTCAGAA ACAGCAAGAA 
ATAGCATCTA TCACAGAGCA GCTTATGACT ACCAAATTAC TTTCAGAACT CCAGAAACTG 
TTTATGAGTA AAAATGAGAC TTATGTGTTA AAATTGTGGC CTTTATTTGT CAAACTTCTT 
GGGAAGACCT TGCATCGAAG TGGGAGTTTC ATTAATTCTC TGTTACAGCT GGAAGAACTG 
GGATTTCGTA GTGGAGCACC CATGATTAAA AAAATAGCTT TTATTGCTTG GAAGAGTTTA 
ATAGATAATT TTGCTTTAAA TCCAGAAATA CTGTGTAGTG CAAAAAGACT GAAGTTGTTG 
ATGCAGCCTT TGAGTTCTAT CCATGTGAGA ACAGAAACTC TAGCGCTGAC AAAACTAGAA 
GTCTGGTGGT ATTTGTTGAT GAGACTAGGA CCTCATCTTC CTGCTAATTT TGAACAGGTT 
TGTGTGCCTC TGATTCAGAG CACAATAAGC ATTGATTCTA ATGCTTCACC TCAAGGGAGT 
TCATCTCGTG TAGCTACTAC TCCAGGTTTA AGCCCTATGA CCCCAGTACA CAAAGGTGCT 
TCTCCATATG GAACTCCTGT AACCCCTCGG ATGAACTTGA ATTCAAATTT TGGAATGGCC 
ACAATCCCCT CTATACAACT TTTGGGGCTT GAAATGTTGC TTCATTTTTT GTTGGGTCCA 
GAAGTCGTGA GTTTTGCTAA GCAAAACAAA CTTATACTGA GCCTAGAACC ACTTGAACAT 
CCATTAATCA GCAGCTCTTC TTTTTTCTCC AAACACTCAA ATACACTTAT CACTGCTGTT 
CATGATAGCT TTGTTGCAGT TGGAAAAGAT GCTTCTGATG TAGTTATTAA TGCTATTTGG 
AAGGAGTTAA TTAGCTTGGT GAAGTCAGTT ATTGAATCAG GTAACAAAAA AGAGAGACCA 
GGTTCTGAAG TTTTGACTCT CTTATTAAAA TCTTTGGAAA GCATAGTGAA TTCAGAAGTA 
TTTCCTGTAT TGAAAACACT GGTTCTCATG GAAATTACAA TTAAAGGACT TCCTCAGAAA 
GTATTAGGTT CTCCAGCATA TCAGGTTGCT AATATGGATA TTCTTAATGG AACCCCTGCT 
TTGTTCTTAA TTCAGTTAAC TTTCAATAAT AATCTATTGG AATGTGGTGT GGAAGACGAA 
AGGTTTTTTC TCAACCTGGA AACACTTGTG GGCTGTGTTC TTTCGGGTCC AACTTCACCA 
CTCGCTTTCA GTGACTCTGT CTTAAATGTT GTCAATCAAA ATGCAAAGCA GTTGGATAAC 
AAGGAGCATC TTTGGAGAAT GTGGAGTATT ATAGTCACCC CATTAACAGA ATTGATTAAT 
CAGACCAATG AAGTGAATCA AGGGGATGCC TTAGAACATA ATTTCAGTGC TATCTATGGT 
GCACTGATTT TGCCAATAAA CCATATTTTT CCAGCACAAA AAATTCCAGT GGTCACCATG 
AAGACCTTAC TTAAAACTTG GTCAGAATTA TATAGAGCAT TTGCTCGCTG TGCAGCTTTG 
GTGGCAACTG CGGAAGAGAA TTTATGCTGT GAGGAATTTT GTTCCAAGAT AATGTCCAGT 
TTGGAAGATG AAGGCCTTTC AAATTTATTG TTCTTGGATA GGATCACTCA TATTATTACT 
GTAATGGTTG ATTGCATCGA CTTTTCACCA TATAATATTA AATATCAACC CAAAACTAAA 
TCACCACAGA GACCTACAGA TTGGTCCAAA AAGAAGAAGG AACCTTTAGG GAAGTTGGCT 
TCTTTAATTA AACTTATTGT GAGAGTGATC TATTCTTTTC ACACACTGAG CCTCAAGGAA 
GTACATTCTG ATACTCTCCT CACTATTGGC AACTCAATCA CCAGCATTCT TTCCAATTTA 
CTTGGACATA TTTCTCTGCC TTCTATGATC CAGAAAATAT TTGCAGCTTT AACAAGACCT 
CTGGCATTAT TTTATGAAAA CTCAAAGCTT GATGAAGTTC CTAAAGTGTA TAGTTGTATG 
AACAACAAGT TAGAAAAGCT ACTAGGAGAT ATAATTGCTT GTCTACATGT CAGCTACACT 
GGAACTTACG ACAGTGAACT TCTTGAACAG ATCTCACCAT TATTATGCAT AATATTTTCA 
CACAAGAACA AACAGATAAG AAAACAAAGT GCTCAGTTCT GGAATGCCAC ATTTGCTAAA 
GTGACGATGT TGATTTATCC TGAGGAGTTA AAGCCAATAC TGAAACAAGC CAAACAAAAA 
TCTCTGCTTT TATTGCCTGG TTTGGAAAAT GTTGAAATTA TGGAGGAATC CAGTGGACCA 
TATTCAGATA TGACAGAAAA TTCCCAATTA AATATGAAGA TAAGTGGCAT GGAAAGAAAA 
TCAAGTGGAA AAAGAGATTC TTTTTTGGCA CAAACAAAGG ATACAAAAGA CAGTATGAAA 
CCACCAGCTA AATTGAAACT AGATTCTTCA ACTCCAAAAG CAAAGGGTGA AATGCCTTTG 
GAAGAAGAAA AGTCTACTGA CTTTGTGTTT ATACCTCCAG AAGTAAAAGA AACAAAGGAA 
AGAATACTAA CTGAACACCA GAAAGAAGTA CTCAAAACAA AGAGGTGTGA TATTCCTGCC 
ATGTATAATA ATCTGGATGT TTCACAAGAT ACTTTATTTT CTCAGTATAG TCAGGAAGAA 
TCTATGGAAA TTTCTACTTT AATTGAAAAA TCAAAAGAAG ACTCCAAGAT GATATTTAAG 
GAGGAACAAA AGGAGAATGA CATTGTCGTT CCTCAACATG TCATGGAAAA CTGCGGTATG 
GATGAACATC TTGAAAAGGC TTCCCTTTTG AATAATGAGT GTGGTTCTAT TGAGGAAACC 
AGTCCAAAAA TACTGAGCAG TAATAATGAT GCCAGAAAAA AAGCTTTAAT TGCTTCAAAT 
AAACCAACAG AATGTGCATC TACTACAGAA AATCCATCTG GTGTCAGCAG TAGCTCGGTT 
TCTATTGCCA CCATTTCTGG AGCTCCCTTA CAGCCTACAA GTCGAAGGCA ATCCTTTATT 
ACTTTGGAAA AGTTTGATGG TTCAGAAAAT AGACCTTTTA GTCCATCACC CTTGAATAAT 
ATGTCATCAA CTGTAATAGT GAAAAGTAAC CAGGAAGGCA TGGGTAAAAC AGATAATCCA 
TCAAAAGCAA AGAAGAGAGA AGTGGCTCTT TCCAAATCTG ACTCTGAAAA AATAACACAT 
GGGACTAAGA GATCAAGCCG AAGGTCGAGT AAATCTGAAC CAATAGGGAG TAAAAAGTCT 
AAACCCTTAA TGAGATCTGA ACAAGAGAAA AGTACTCAAG AATCTGTTGA TGGCACAGTA 
GCCTTAGAAA ATAACCCACC TGGCTTGCTT AATCAGACAG AATGTGTGTT AGATAATCAG 
GTTCATCTCT CTGAATCTAC AGTGGAGTTT GAGGATACAA TGCTTGAATC AACAGTGGAG 
AATACTGTAT TAGAAAACAA TACTGTAGAA GAGAGAACCT TAGAAGTTAA CTTGGAATCC 
AAAGAAAACA CACCCCCAGC CATAATAACG GATCAGACAG TAAATGAGGA TAGTCATATT 
CAGGTAACTC CAAATCAGAA AACCCTTAGG CGATCTTTAA GGCGACGTTC AGAAACAGCA 
GAACCAACCA CTGATAGCCA AGATAAAGAA AATAATCATC AAAAAAGGGA AAGACGTAAA 
GAAGAAGAAA AGACTCCGCA GAAGAGTCCG TTACATGTAA AAGATGATGT TTTACCTAAG 
CAAAAATTGA TTTCTGAACA AAGTGTACAG GGAAACTTAG TTGACAAAGG AAATAATTTA 
CATGAGAAGG CTTTTGGGGA GACCAGTGCT AATGCTGAAA TTGATGAAAA TAGAAGAAAG 
CCAGATCTTG AGAATACTAA ATCTGAAGGA GATGGTGTGC CGGACATTGC AGATAAATCC 
TCTGAGAAAC CAGTAAGGGG ACGAACACGA TACCAAACAA GAAGAGCATC CCAGGGTTTA 
CTTTCCAGCA TTGAAAACTC AGAATCTGAT AGTTCTGAGG CAAAAGAAGA AGGTTCTAAA 
AAGAAAAGAT CTGGAAAATG GAAGAACAAA AGCAGTGACA ATGTTGACAT GGAAGATCAA 
GAAGAGAAAA TGGTAAAACA GGAATGTATA AAAATTGAAA ATCAGGCACA TGATTATAAA 
GGGAATCCTG AAGTAGACAA AGATATAAAA TCTCAAATCT GTGAAAAAGG AGATGAAAGT 
ACTACTGTAA GTCTTCAAGA TTCTACCGTA TCTCCAGATT TATTACAAGT TTATGATGAT 
GTATTGAATA CTTCTGAGGG AGAAAGTAAA AGTAACAAAG ATGCAGACCA TTCCTTTTCA 
AATCCTTCTG TACCAGAATC AAATCTAAGG ACTAGAAATG CCAGTAAGAG GTTACATAAG 
CGAGATTCTA TAGAAAATAG CATGGGGGAA TCCTCAAAAA TAGGGACATC AGACATTTCT 
TTGCTTTCTG AAAAAACTCT TCAGACAGTA GAATGCCAGC ACAAGAGAAG TAGGAGGGTG 
AGGCGATCTA AAGGTTGTGA TTGTTGTGGA GAAAAATCAC AGTCTCAGGA AAAGTCATTT 
ACTGGGTTAA AGGATACAGA AAATTACGAT GTAAAAGTCA GTGAAACAAA AAAGACAGAT 
GGGGAAACAC CTGTATCTAT TTCAGAAACT TCGAAAGCTG ATCTGTCCTC GGAGGTTAAA 
CTTTTAGATG AGCATCATAG TGTGAATTTT CATTTGGATC TCAAGGAAGA GAATGATACT 
ACTAATGATT CATTAATTAT ATCTGAAACT GAGTTAAAAG AAAATACTAC TCAAAGCTTT 
CTTCCTTCTG AAATGGTAAA ATTTAAAGAA ACTTCTCGTA GGGATTCTGA GGAAGCAATA 
TCTCTAGAAA CCCAGGAGCC ATCTAATAAG AAATGTAAAA CTGTTGACCC ACATTTAGAT 
GTTTCCAAAG ATATTTCACT GAAATGTTTT TCTTTGGAGA CTAAAGAAGA AAAGTCAGAG 
GCCACTTCAA AAGAGAAATT GAGTACAGAG AACATTGTTA TTGTTGAATC AAATGCAGAA 
TCAAATCCAG AAGTAGATGC TATGGAATTG AATGTAGAAA ATGATCAGTC TGAAGCTGGC 
TTCTCTGAAC AAAAGAATGT CAAAACTGGT ATTCCAGGGA AAGAAGTAAC AGAGGAAAAC 
AGTGAAAGAA TGGATACACC TGAAAAACTG AAAGCCAATG AAGCAGTAAC TGAGGAATTT 
AATTCAGCTA CTGATTATTC GGATACTCCG ACATCTGTAA AAGTGAATGC TCAGGTAGAG 
ATCTCTGAAC AAATAGCGGT TGGGGAGCTA GGTGGAGGAA GTGATGAATC TGATCCAGGC 
TCATCTGAAG AAATGAACGC TGAAATGAAA AATTGTGAAA AAACAGCGGT TTGTGAGGTG 
ACTACTGAAT CTGACCACGT CACTGAAACC GAAGACGAAG ATGTAACTAC CACGGCTTCT 
CTGCCTATAG AAGTTGAAAC AAAGGAATTA GATGTAGAAG TCAATAACTT TGTTCCCGAC 
ACACTTGAGA TGATCACTGA AGAAGGAAAG GTTGACCCTA ACAAAACTGA AACGAATACT 
GAACCTAATA AATTGGAGGA AACACAGTTA GATGACAATC AGATGGTAGT GGAAAGTAAT 
GAAACTTTAC CGGAAGTTCA CCATACTTCA GAGAAAGTAG AGGAGATAGC ACAACCTCTG 
ACATCTGAAA CAGATATTTC TGGACTAATA TCAGAAGACA GTAATACATC TCCTCAAAAA 
TCGAAGGATA TTGATCCTTT ACTCATGTCA GCAAACGAGA GTCCTAGTGG CATGCACACA 
CGTTGTGTGT GGTCTCCTTT GGCTTCTCCA TCCACCAGCA TTTTAAAGAG AGGACTAAAA 
AGACCCCAAG AAGATGAAAT CTCGTCACCT GTCCACAAGA TTGCAGAAAT GGCTAAGGAA 
TCTGTGCCAT GCCCAACAGA AAGTGTTTAC CCTGCTTTGG TGAACTGTGT AGCACCAGTT 
GAAATCATTT TACCTCAGAT TACATCCAAC ATGTGGGCAA GAGGACTGGG ACAGCTCATT 
AGAGCCAAGA ATATAAAAAC AATTGGCGAT TTGAGTACCC TTACAGCATC TGAAATAAAA 
ACTCTTCCTA TCCGTTCTCC AAAAGTGTCT AACGTTAAAA AGGCTCTCAG AATATATCAT 
GAGCAGCAGG TGAAGTCTCG TGGACTAGAA GAGATTCCAG TTTTTGATAT TTCTGAAAAA 
ACAATAAATG GAATGGAAAA TAAATCTATC TCTCCTGATG AAGAAAGACT TGCCTCAGAT 
TTAATTGATC CTGTTGCTTT AGAAGCTCCG TTATCTAAAA ATCTTGTGGC ACAGATTAGC 
GCACTTGCTC TTCAACTAGA TTCAGAAGAT CTCTATAATT ATTCAGGAAG CCAGCTATTT 
GAAATGCACG AGAAACTTGG TAGCATGGCA AACTCTATAA TTAAAAATCT GCAGTCACGT 
TGGAGGTCAC CAGCCCATGA AAATTCTTTT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RIF1 ( XM_010802077.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010800379.1 Bos taurus replication timing regulatory factor 1 (RIF1), transcript variant X7, mRNA.

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CloneID	OBa55068
Clone ID Related Accession (Same CDS sequence)	XM_010826493.2 , XM_010802077.3 , XM_010802077.2
Accession Version	XM_010802077.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7173bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	telomere-associated protein RIF1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010802077.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGCCG CGGATCAGAG CCCCCTGGCA CCGCTGTTGG AGACTTTGGA AGACCCTTCC 
GCCTCCCTTG GAGAGCAGAC CGACGCTTAC CTGACCCTGA CCAGTCGTAT GACTGGAGAA 
GATGGAAAAG AAATCATTAC AGAAATTGAG AAAAAACTTC CTCAGCTATT TAAAGTTTTA 
AAGACTCACA TTGCCAGTCA AAACTCAGAG CTCAGTAGTG CTGCTCTACA GGCCTTGGGG 
TTTTGCTTAT ATAATCCCAA AATTGCCTCT GGATTATCAG AGATAAATAT TCAAGAACTG 
CTTTCAAAAC TGAATGATAT TGTTAAAAGT TCAGATAAAA ATGTACGTAC TAGGGCACTT 
TGGGTGATAT CCAAGCAGAC GTTTCCCACT GAAGTCGTTG GCAAAGTAGT TTCTAGTATC 
ATTGACTCAC TAGAAATAGT ATTTAATAGA GGAGAGATGC TTTCTGCTGT TGTGGATTAT 
GAAGCATTAA ATGTCATTAT AAGACTAATT GAACAAGCCC CAGTTCAAAT GGGAGAAGAG 
TCAATTAGGT GGGCAAAATT GGTCATACCT TTACTGGTTC ATTCAGCCCA AAAAGTACAT 
TTGCGGGGAG CCACTGCTCT AGAGATGGGA ATGCCTTTGT TGCTTCAGAA ACAGCAAGAA 
ATAGCATCTA TCACAGAGCA GCTTATGACT ACCAAATTAC TTTCAGAACT CCAGAAACTG 
TTTATGAGTA AAAATGAGAC TTATGTGTTA AAATTGTGGC CTTTATTTGT CAAACTTCTT 
GGGAAGACCT TGCATCGAAG TGGGAGTTTC ATTAATTCTC TGTTACAGCT GGAAGAACTG 
GGATTTCGTA GTGGAGCACC CATGATTAAA AAAATAGCTT TTATTGCTTG GAAGAGTTTA 
ATAGATAATT TTGCTTTAAA TCCAGAAATA CTGTGTAGTG CAAAAAGACT GAAGTTGTTG 
ATGCAGCCTT TGAGTTCTAT CCATGTGAGA ACAGAAACTC TAGCGCTGAC AAAACTAGAA 
GTCTGGTGGT ATTTGTTGAT GAGACTAGGA CCTCATCTTC CTGCTAATTT TGAACAGGTT 
TGTGTGCCTC TGATTCAGAG CACAATAAGC ATTGATTCTA ATGCTTCACC TCAAGGGAGT 
TCATCTCGTG TAGCTACTAC TCCAGGTTTA AGCCCTATGA CCCCAGTACA CAAAGGTGCT 
TCTCCATATG GAACTCCTGT AACCCCTCGG ATGAACTTGA ATTCAAATTT TGGAATGGCC 
ACAATCCCCT CTATACAACT TTTGGGGCTT GAAATGTTGC TTCATTTTTT GTTGGGTCCA 
GAAGTCGTGA GTTTTGCTAA GCAAAACAAA CTTATACTGA GCCTAGAACC ACTTGAACAT 
CCATTAATCA GCAGCTCTTC TTTTTTCTCC AAACACTCAA ATACACTTAT CACTGCTGTT 
CATGATAGCT TTGTTGCAGT TGGAAAAGAT GCTTCTGATG TAGTTATTAA TGCTATTTGG 
AAGGAGTTAA TTAGCTTGGT GAAGTCAGTT ATTGAATCAG GTAACAAAAA AGAGAGACCA 
GGTTCTGAAG TTTTGACTCT CTTATTAAAA TCTTTGGAAA GCATAGTGAA TTCAGAAGTA 
TTTCCTGTAT TGAAAACACT GGTTCTCATG GAAATTACAA TTAAAGGACT TCCTCAGAAA 
GTATTAGGTT CTCCAGCATA TCAGGTTGCT AATATGGATA TTCTTAATGG AACCCCTGCT 
TTGTTCTTAA TTCAGTTAAC TTTCAATAAT AATCTATTGG AATGTGGTGT GGAAGACGAA 
AGGTTTTTTC TCAACCTGGA AACACTTGTG GGCTGTGTTC TTTCGGGTCC AACTTCACCA 
CTCGCTTTCA GTGACTCTGT CTTAAATGTT GTCAATCAAA ATGCAAAGCA GTTGGATAAC 
AAGGAGCATC TTTGGAGAAT GTGGAGTATT ATAGTCACCC CATTAACAGA ATTGATTAAT 
CAGACCAATG AAGTGAATCA AGGGGATGCC TTAGAACATA ATTTCAGTGC TATCTATGGT 
GCACTGATTT TGCCAATAAA CCATATTTTT CCAGCACAAA AAATTCCAGT GGTCACCATG 
AAGACCTTAC TTAAAACTTG GTCAGAATTA TATAGAGCAT TTGCTCGCTG TGCAGCTTTG 
GTGGCAACTG CGGAAGAGAA TTTATGCTGT GAGGAATTTT GTTCCAAGAT AATGTCCAGT 
TTGGAAGATG AAGGCCTTTC AAATTTATTG TTCTTGGATA GGATCACTCA TATTATTACT 
GTAATGGTTG ATTGCATCGA CTTTTCACCA TATAATATTA AATATCAACC CAAAACTAAA 
TCACCACAGA GACCTACAGA TTGGTCCAAA AAGAAGAAGG AACCTTTAGG GAAGTTGGCT 
TCTTTAATTA AACTTATTGT GAGAGTGATC TATTCTTTTC ACACACTGAG CCTCAAGGAA 
GTACATTCTG ATACTCTCCT CACTATTGGC AACTCAATCA CCAGCATTCT TTCCAATTTA 
CTTGGACATA TTTCTCTGCC TTCTATGATC CAGAAAATAT TTGCAGCTTT AACAAGACCT 
CTGGCATTAT TTTATGAAAA CTCAAAGCTT GATGAAGTTC CTAAAGTGTA TAGTTGTATG 
AACAACAAGT TAGAAAAGCT ACTAGGAGAT ATAATTGCTT GTCTACATGT CAGCTACACT 
GGAACTTACG ACAGTGAACT TCTTGAACAG ATCTCACCAT TATTATGCAT AATATTTTCA 
CACAAGAACA AACAGATAAG AAAACAAAGT GCTCAGTTCT GGAATGCCAC ATTTGCTAAA 
GTGACGATGT TGATTTATCC TGAGGAGTTA AAGCCAATAC TGAAACAAGC CAAACAAAAA 
TCTCTGCTTT TATTGCCTGG TTTGGAAAAT GTTGAAATTA TGGAGGAATC CAGTGGACCA 
TATTCAGATA TGACAGAAAA TTCCCAATTA AATATGAAGA TAAGTGGCAT GGAAAGAAAA 
TCAAGTGGAA AAAGAGATTC TTTTTTGGCA CAAACAAAGG ATACAAAAGA CAGTATGAAA 
CCACCAGCTA AATTGAAACT AGATTCTTCA ACTCCAAAAG CAAAGGGTGA AATGCCTTTG 
GAAGAAGAAA AGTCTACTGA CTTTGTGTTT ATACCTCCAG AAGTAAAAGA AACAAAGGAA 
AGAATACTAA CTGAACACCA GAAAGAAGTA CTCAAAACAA AGAGGTGTGA TATTCCTGCC 
ATGTATAATA ATCTGGATGT TTCACAAGAT ACTTTATTTT CTCAGTATAG TCAGGAAGAA 
TCTATGGAAA TTTCTACTTT AATTGAAAAA TCAAAAGAAG ACTCCAAGAT GATATTTAAG 
GAGGAACAAA AGGAGAATGA CATTGTCGTT CCTCAACATG TCATGGAAAA CTGCGGTATG 
GATGAACATC TTGAAAAGGC TTCCCTTTTG AATAATGAGT GTGGTTCTAT TGAGGAAACC 
AGTCCAAAAA TACTGAGCAG TAATAATGAT GCCAGAAAAA AAGCTTTAAT TGCTTCAAAT 
AAACCAACAG AATGTGCATC TACTACAGAA AATCCATCTG GTGTCAGCAG TAGCTCGGTT 
TCTATTGCCA CCATTTCTGG AGCTCCCTTA CAGCCTACAA GTCGAAGGCA ATCCTTTATT 
ACTTTGGAAA AGTTTGATGG TTCAGAAAAT AGACCTTTTA GTCCATCACC CTTGAATAAT 
ATGTCATCAA CTGTAATAGT GAAAAGTAAC CAGGAAGGCA TGGGTAAAAC AGATAATCCA 
TCAAAAGCAA AGAAGAGAGA AGTGGCTCTT TCCAAATCTG ACTCTGAAAA AATAACACAT 
GGGACTAAGA GATCAAGCCG AAGGTCGAGT AAATCTGAAC CAATAGGGAG TAAAAAGTCT 
AAACCCTTAA TGAGATCTGA ACAAGAGAAA AGTACTCAAG AATCTGTTGA TGGCACAGTA 
GCCTTAGAAA ATAACCCACC TGGCTTGCTT AATCAGACAG AATGTGTGTT AGATAATCAG 
GTTCATCTCT CTGAATCTAC AGTGGAGTTT GAGGATACAA TGCTTGAATC AACAGTGGAG 
AATACTGTAT TAGAAAACAA TACTGTAGAA GAGAGAACCT TAGAAGTTAA CTTGGAATCC 
AAAGAAAACA CACCCCCAGC CATAATAACG GATCAGACAG TAAATGAGGA TAGTCATATT 
CAGGTAACTC CAAATCAGAA AACCCTTAGG CGATCTTTAA GGCGACGTTC AGAAACAGCA 
GAACCAACCA CTGATAGCCA AGATAAAGAA AATAATCATC AAAAAAGGGA AAGACGTAAA 
GAAGAAGAAA AGACTCCGCA GAAGAGTCCG TTACATGTAA AAGATGATGT TTTACCTAAG 
CAAAAATTGA TTTCTGAACA AAGTGTACAG GGAAACTTAG TTGACAAAGG AAATAATTTA 
CATGAGAAGG CTTTTGGGGA GACCAGTGCT AATGCTGAAA TTGATGAAAA TAGAAGAAAG 
CCAGATCTTG AGAATACTAA ATCTGAAGGA GATGGTGTGC CGGACATTGC AGATAAATCC 
TCTGAGAAAC CAGTAAGGGG ACGAACACGA TACCAAACAA GAAGAGCATC CCAGGGTTTA 
CTTTCCAGCA TTGAAAACTC AGAATCTGAT AGTTCTGAGG CAAAAGAAGA AGGTTCTAAA 
AAGAAAAGAT CTGGAAAATG GAAGAACAAA AGCAGTGACA ATGTTGACAT GGAAGATCAA 
GAAGAGAAAA TGGTAAAACA GGAATGTATA AAAATTGAAA ATCAGGCACA TGATTATAAA 
GGGAATCCTG AAGTAGACAA AGATATAAAA TCTCAAATCT GTGAAAAAGG AGATGAAAGT 
ACTACTGTAA GTCTTCAAGA TTCTACCGTA TCTCCAGATT TATTACAAGT TTATGATGAT 
GTATTGAATA CTTCTGAGGG AGAAAGTAAA AGTAACAAAG ATGCAGACCA TTCCTTTTCA 
AATCCTTCTG TACCAGAATC AAATCTAAGG ACTAGAAATG CCAGTAAGAG GTTACATAAG 
CGAGATTCTA TAGAAAATAG CATGGGGGAA TCCTCAAAAA TAGGGACATC AGACATTTCT 
TTGCTTTCTG AAAAAACTCT TCAGACAGTA GAATGCCAGC ACAAGAGAAG TAGGAGGGTG 
AGGCGATCTA AAGGTTGTGA TTGTTGTGGA GAAAAATCAC AGTCTCAGGA AAAGTCATTT 
ACTGGGTTAA AGGATACAGA AAATTACGAT GTAAAAGTCA GTGAAACAAA AAAGACAGAT 
GGGGAAACAC CTGTATCTAT TTCAGAAACT TCGAAAGCTG ATCTGTCCTC GGAGGTTAAA 
CTTTTAGATG AGCATCATAG TGTGAATTTT CATTTGGATC TCAAGGAAGA GAATGATACT 
ACTAATGATT CATTAATTAT ATCTGAAACT GAGTTAAAAG AAAATACTAC TCAAAGCTTT 
CTTCCTTCTG AAATGGTAAA ATTTAAAGAA ACTTCTCGTA GGGATTCTGA GGAAGCAATA 
TCTCTAGAAA CCCAGGAGCC ATCTAATAAG AAATGTAAAA CTGTTGACCC ACATTTAGAT 
GTTTCCAAAG ATATTTCACT GAAATGTTTT TCTTTGGAGA CTAAAGAAGA AAAGTCAGAG 
GCCACTTCAA AAGAGAAATT GAGTACAGAG AACATTGTTA TTGTTGAATC AAATGCAGAA 
TCAAATCCAG AAGTAGATGC TATGGAATTG AATGTAGAAA ATGATCAGTC TGAAGCTGGC 
TTCTCTGAAC AAAAGAATGT CAAAACTGGT ATTCCAGGGA AAGAAGTAAC AGAGGAAAAC 
AGTGAAAGAA TGGATACACC TGAAAAACTG AAAGCCAATG AAGCAGTAAC TGAGGAATTT 
AATTCAGCTA CTGATTATTC GGATACTCCG ACATCTGTAA AAGTGAATGC TCAGGTAGAG 
ATCTCTGAAC AAATAGCGGT TGGGGAGCTA GGTGGAGGAA GTGATGAATC TGATCCAGGC 
TCATCTGAAG AAATGAACGC TGAAATGAAA AATTGTGAAA AAACAGCGGT TTGTGAGGTG 
ACTACTGAAT CTGACCACGT CACTGAAACC GAAGACGAAG ATGTAACTAC CACGGCTTCT 
CTGCCTATAG AAGTTGAAAC AAAGGAATTA GATGTAGAAG TCAATAACTT TGTTCCCGAC 
ACACTTGAGA TGATCACTGA AGAAGGAAAG GTTGACCCTA ACAAAACTGA AACGAATACT 
GAACCTAATA AATTGGAGGA AACACAGTTA GATGACAATC AGATGGTAGT GGAAAGTAAT 
GAAACTTTAC CGGAAGTTCA CCATACTTCA GAGAAAGTAG AGGAGATAGC ACAACCTCTG 
ACATCTGAAA CAGATATTTC TGGACTAATA TCAGAAGACA GTAATACATC TCCTCAAAAA 
TCGAAGGATA TTGATCCTTT ACTCATGTCA GCAAACGAGA GTCCTAGTGG CATGCACACA 
CGTTGTGTGT GGTCTCCTTT GGCTTCTCCA TCCACCAGCA TTTTAAAGAG AGGACTAAAA 
AGACCCCAAG AAGATGAAAT CTCGTCACCT GTCCACAAGA TTGCAGAAAT GGCTAAGGAA 
TCTGTGCCAT GCCCAACAGA AAGTGTTTAC CCTGCTTTGG TGAACTGTGT AGCACCAGTT 
GAAATCATTT TACCTCAGAT TACATCCAAC ATGTGGGCAA GAGGACTGGG ACAGCTCATT 
AGAGCCAAGA ATATAAAAAC AATTGGCGAT TTGAGTACCC TTACAGCATC TGAAATAAAA 
ACTCTTCCTA TCCGTTCTCC AAAAGTGTCT AACGTTAAAA AGGCTCTCAG AATATATCAT 
GAGCAGCAGG TGAAGTCTCG TGGACTAGAA GAGATTCCAG TTTTTGATAT TTCTGAAAAA 
ACAATAAATG GAATGGAAAA TAAATCTATC TCTCCTGATG AAGAAAGACT TGCCTCAGAT 
TTAATTGATC CTGTTGCTTT AGAAGCTCCG TTATCTAAAA ATCTTGTGGC ACAGATTAGC 
GCACTTGCTC TTCAACTAGA TTCAGAAGAT CTCTATAATT ATTCAGGAAG CCAGCTATTT 
GAAATGCACG AGAAACTTGG TAGCATGGCA AACTCTATAA TTAAAAATCT GCAGTCACGT 
TGGAGGTCAC CAGCCCATGA AAATTCTTTT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010800379.1
CDS	171..7343
Translation	MTAADQSPLAPLLETLEDPSASLGEQTDAYLTLTSRMTGEDGKEIITEIEKKLPQLFKVLKTHIASQNSELSSAALQALGFCLYNPKIASGLSEINIQELLSKLNDIVKSSDKNVRTRALWVISKQTFPTEVVGKVVSSIIDSLEIVFNRGEMLSAVVDYEALNVIIRLIEQAPVQMGEESIRWAKLVIPLLVHSAQKVHLRGATALEMGMPLLLQKQQEIASITEQLMTTKLLSELQKLFMSKNETYVLKLWPLFVKLLGKTLHRSGSFINSLLQLEELGFRSGAPMIKKIAFIAWKSLIDNFALNPEILCSAKRLKLLMQPLSSIHVRTETLALTKLEVWWYLLMRLGPHLPANFEQVCVPLIQSTISIDSNASPQGSSSRVATTPGLSPMTPVHKGASPYGTPVTPRMNLNSNFGMATIPSIQLLGLEMLLHFLLGPEVVSFAKQNKLILSLEPLEHPLISSSSFFSKHSNTLITAVHDSFVAVGKDASDVVINAIWKELISLVKSVIESGNKKERPGSEVLTLLLKSLESIVNSEVFPVLKTLVLMEITIKGLPQKVLGSPAYQVANMDILNGTPALFLIQLTFNNNLLECGVEDERFFLNLETLVGCVLSGPTSPLAFSDSVLNVVNQNAKQLDNKEHLWRMWSIIVTPLTELINQTNEVNQGDALEHNFSAIYGALILPINHIFPAQKIPVVTMKTLLKTWSELYRAFARCAALVATAEENLCCEEFCSKIMSSLEDEGLSNLLFLDRITHIITVMVDCIDFSPYNIKYQPKTKSPQRPTDWSKKKKEPLGKLASLIKLIVRVIYSFHTLSLKEVHSDTLLTIGNSITSILSNLLGHISLPSMIQKIFAALTRPLALFYENSKLDEVPKVYSCMNNKLEKLLGDIIACLHVSYTGTYDSELLEQISPLLCIIFSHKNKQIRKQSAQFWNATFAKVTMLIYPEELKPILKQAKQKSLLLLPGLENVEIMEESSGPYSDMTENSQLNMKISGMERKSSGKRDSFLAQTKDTKDSMKPPAKLKLDSSTPKAKGEMPLEEEKSTDFVFIPPEVKETKERILTEHQKEVLKTKRCDIPAMYNNLDVSQDTLFSQYSQEESMEISTLIEKSKEDSKMIFKEEQKENDIVVPQHVMENCGMDEHLEKASLLNNECGSIEETSPKILSSNNDARKKALIASNKPTECASTTENPSGVSSSSVSIATISGAPLQPTSRRQSFITLEKFDGSENRPFSPSPLNNMSSTVIVKSNQEGMGKTDNPSKAKKREVALSKSDSEKITHGTKRSSRRSSKSEPIGSKKSKPLMRSEQEKSTQESVDGTVALENNPPGLLNQTECVLDNQVHLSESTVEFEDTMLESTVENTVLENNTVEERTLEVNLESKENTPPAIITDQTVNEDSHIQVTPNQKTLRRSLRRRSETAEPTTDSQDKENNHQKRERRKEEEKTPQKSPLHVKDDVLPKQKLISEQSVQGNLVDKGNNLHEKAFGETSANAEIDENRRKPDLENTKSEGDGVPDIADKSSEKPVRGRTRYQTRRASQGLLSSIENSESDSSEAKEEGSKKKRSGKWKNKSSDNVDMEDQEEKMVKQECIKIENQAHDYKGNPEVDKDIKSQICEKGDESTTVSLQDSTVSPDLLQVYDDVLNTSEGESKSNKDADHSFSNPSVPESNLRTRNASKRLHKRDSIENSMGESSKIGTSDISLLSEKTLQTVECQHKRSRRVRRSKGCDCCGEKSQSQEKSFTGLKDTENYDVKVSETKKTDGETPVSISETSKADLSSEVKLLDEHHSVNFHLDLKEENDTTNDSLIISETELKENTTQSFLPSEMVKFKETSRRDSEEAISLETQEPSNKKCKTVDPHLDVSKDISLKCFSLETKEEKSEATSKEKLSTENIVIVESNAESNPEVDAMELNVENDQSEAGFSEQKNVKTGIPGKEVTEENSERMDTPEKLKANEAVTEEFNSATDYSDTPTSVKVNAQVEISEQIAVGELGGGSDESDPGSSEEMNAEMKNCEKTAVCEVTTESDHVTETEDEDVTTTASLPIEVETKELDVEVNNFVPDTLEMITEEGKVDPNKTETNTEPNKLEETQLDDNQMVVESNETLPEVHHTSEKVEEIAQPLTSETDISGLISEDSNTSPQKSKDIDPLLMSANESPSGMHTRCVWSPLASPSTSILKRGLKRPQEDEISSPVHKIAEMAKESVPCPTESVYPALVNCVAPVEIILPQITSNMWARGLGQLIRAKNIKTIGDLSTLTASEIKTLPIRSPKVSNVKKALRIYHEQQVKSRGLEEIPVFDISEKTINGMENKSISPDEERLASDLIDPVALEAPLSKNLVAQISALALQLDSEDLYNYSGSQLFEMHEKLGSMANSIIKNLQSRWRSPAHENSF

Target ORF information:

RefSeq Version	XM_010802077.3
Organism	Bos taurus(cattle)
Definition	Bos taurus replication timing regulatory factor 1 (RIF1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010802077.3

ORF Insert Sequence:

ATGACGGCCG CGGATCAGAG CCCCCTGGCA CCGCTGTTGG AGACTTTGGA AGACCCTTCC 
GCCTCCCTTG GAGAGCAGAC CGACGCTTAC CTGACCCTGA CCAGTCGTAT GACTGGAGAA 
GATGGAAAAG AAATCATTAC AGAAATTGAG AAAAAACTTC CTCAGCTATT TAAAGTTTTA 
AAGACTCACA TTGCCAGTCA AAACTCAGAG CTCAGTAGTG CTGCTCTACA GGCCTTGGGG 
TTTTGCTTAT ATAATCCCAA AATTGCCTCT GGATTATCAG AGATAAATAT TCAAGAACTG 
CTTTCAAAAC TGAATGATAT TGTTAAAAGT TCAGATAAAA ATGTACGTAC TAGGGCACTT 
TGGGTGATAT CCAAGCAGAC GTTTCCCACT GAAGTCGTTG GCAAAGTAGT TTCTAGTATC 
ATTGACTCAC TAGAAATAGT ATTTAATAGA GGAGAGATGC TTTCTGCTGT TGTGGATTAT 
GAAGCATTAA ATGTCATTAT AAGACTAATT GAACAAGCCC CAGTTCAAAT GGGAGAAGAG 
TCAATTAGGT GGGCAAAATT GGTCATACCT TTACTGGTTC ATTCAGCCCA AAAAGTACAT 
TTGCGGGGAG CCACTGCTCT AGAGATGGGA ATGCCTTTGT TGCTTCAGAA ACAGCAAGAA 
ATAGCATCTA TCACAGAGCA GCTTATGACT ACCAAATTAC TTTCAGAACT CCAGAAACTG 
TTTATGAGTA AAAATGAGAC TTATGTGTTA AAATTGTGGC CTTTATTTGT CAAACTTCTT 
GGGAAGACCT TGCATCGAAG TGGGAGTTTC ATTAATTCTC TGTTACAGCT GGAAGAACTG 
GGATTTCGTA GTGGAGCACC CATGATTAAA AAAATAGCTT TTATTGCTTG GAAGAGTTTA 
ATAGATAATT TTGCTTTAAA TCCAGAAATA CTGTGTAGTG CAAAAAGACT GAAGTTGTTG 
ATGCAGCCTT TGAGTTCTAT CCATGTGAGA ACAGAAACTC TAGCGCTGAC AAAACTAGAA 
GTCTGGTGGT ATTTGTTGAT GAGACTAGGA CCTCATCTTC CTGCTAATTT TGAACAGGTT 
TGTGTGCCTC TGATTCAGAG CACAATAAGC ATTGATTCTA ATGCTTCACC TCAAGGGAGT 
TCATCTCGTG TAGCTACTAC TCCAGGTTTA AGCCCTATGA CCCCAGTACA CAAAGGTGCT 
TCTCCATATG GAACTCCTGT AACCCCTCGG ATGAACTTGA ATTCAAATTT TGGAATGGCC 
ACAATCCCCT CTATACAACT TTTGGGGCTT GAAATGTTGC TTCATTTTTT GTTGGGTCCA 
GAAGTCGTGA GTTTTGCTAA GCAAAACAAA CTTATACTGA GCCTAGAACC ACTTGAACAT 
CCATTAATCA GCAGCTCTTC TTTTTTCTCC AAACACTCAA ATACACTTAT CACTGCTGTT 
CATGATAGCT TTGTTGCAGT TGGAAAAGAT GCTTCTGATG TAGTTATTAA TGCTATTTGG 
AAGGAGTTAA TTAGCTTGGT GAAGTCAGTT ATTGAATCAG GTAACAAAAA AGAGAGACCA 
GGTTCTGAAG TTTTGACTCT CTTATTAAAA TCTTTGGAAA GCATAGTGAA TTCAGAAGTA 
TTTCCTGTAT TGAAAACACT GGTTCTCATG GAAATTACAA TTAAAGGACT TCCTCAGAAA 
GTATTAGGTT CTCCAGCATA TCAGGTTGCT AATATGGATA TTCTTAATGG AACCCCTGCT 
TTGTTCTTAA TTCAGTTAAC TTTCAATAAT AATCTATTGG AATGTGGTGT GGAAGACGAA 
AGGTTTTTTC TCAACCTGGA AACACTTGTG GGCTGTGTTC TTTCGGGTCC AACTTCACCA 
CTCGCTTTCA GTGACTCTGT CTTAAATGTT GTCAATCAAA ATGCAAAGCA GTTGGATAAC 
AAGGAGCATC TTTGGAGAAT GTGGAGTATT ATAGTCACCC CATTAACAGA ATTGATTAAT 
CAGACCAATG AAGTGAATCA AGGGGATGCC TTAGAACATA ATTTCAGTGC TATCTATGGT 
GCACTGATTT TGCCAATAAA CCATATTTTT CCAGCACAAA AAATTCCAGT GGTCACCATG 
AAGACCTTAC TTAAAACTTG GTCAGAATTA TATAGAGCAT TTGCTCGCTG TGCAGCTTTG 
GTGGCAACTG CGGAAGAGAA TTTATGCTGT GAGGAATTTT GTTCCAAGAT AATGTCCAGT 
TTGGAAGATG AAGGCCTTTC AAATTTATTG TTCTTGGATA GGATCACTCA TATTATTACT 
GTAATGGTTG ATTGCATCGA CTTTTCACCA TATAATATTA AATATCAACC CAAAACTAAA 
TCACCACAGA GACCTACAGA TTGGTCCAAA AAGAAGAAGG AACCTTTAGG GAAGTTGGCT 
TCTTTAATTA AACTTATTGT GAGAGTGATC TATTCTTTTC ACACACTGAG CCTCAAGGAA 
GTACATTCTG ATACTCTCCT CACTATTGGC AACTCAATCA CCAGCATTCT TTCCAATTTA 
CTTGGACATA TTTCTCTGCC TTCTATGATC CAGAAAATAT TTGCAGCTTT AACAAGACCT 
CTGGCATTAT TTTATGAAAA CTCAAAGCTT GATGAAGTTC CTAAAGTGTA TAGTTGTATG 
AACAACAAGT TAGAAAAGCT ACTAGGAGAT ATAATTGCTT GTCTACATGT CAGCTACACT 
GGAACTTACG ACAGTGAACT TCTTGAACAG ATCTCACCAT TATTATGCAT AATATTTTCA 
CACAAGAACA AACAGATAAG AAAACAAAGT GCTCAGTTCT GGAATGCCAC ATTTGCTAAA 
GTGACGATGT TGATTTATCC TGAGGAGTTA AAGCCAATAC TGAAACAAGC CAAACAAAAA 
TCTCTGCTTT TATTGCCTGG TTTGGAAAAT GTTGAAATTA TGGAGGAATC CAGTGGACCA 
TATTCAGATA TGACAGAAAA TTCCCAATTA AATATGAAGA TAAGTGGCAT GGAAAGAAAA 
TCAAGTGGAA AAAGAGATTC TTTTTTGGCA CAAACAAAGG ATACAAAAGA CAGTATGAAA 
CCACCAGCTA AATTGAAACT AGATTCTTCA ACTCCAAAAG CAAAGGGTGA AATGCCTTTG 
GAAGAAGAAA AGTCTACTGA CTTTGTGTTT ATACCTCCAG AAGTAAAAGA AACAAAGGAA 
AGAATACTAA CTGAACACCA GAAAGAAGTA CTCAAAACAA AGAGGTGTGA TATTCCTGCC 
ATGTATAATA ATCTGGATGT TTCACAAGAT ACTTTATTTT CTCAGTATAG TCAGGAAGAA 
TCTATGGAAA TTTCTACTTT AATTGAAAAA TCAAAAGAAG ACTCCAAGAT GATATTTAAG 
GAGGAACAAA AGGAGAATGA CATTGTCGTT CCTCAACATG TCATGGAAAA CTGCGGTATG 
GATGAACATC TTGAAAAGGC TTCCCTTTTG AATAATGAGT GTGGTTCTAT TGAGGAAACC 
AGTCCAAAAA TACTGAGCAG TAATAATGAT GCCAGAAAAA AAGCTTTAAT TGCTTCAAAT 
AAACCAACAG AATGTGCATC TACTACAGAA AATCCATCTG GTGTCAGCAG TAGCTCGGTT 
TCTATTGCCA CCATTTCTGG AGCTCCCTTA CAGCCTACAA GTCGAAGGCA ATCCTTTATT 
ACTTTGGAAA AGTTTGATGG TTCAGAAAAT AGACCTTTTA GTCCATCACC CTTGAATAAT 
ATGTCATCAA CTGTAATAGT GAAAAGTAAC CAGGAAGGCA TGGGTAAAAC AGATAATCCA 
TCAAAAGCAA AGAAGAGAGA AGTGGCTCTT TCCAAATCTG ACTCTGAAAA AATAACACAT 
GGGACTAAGA GATCAAGCCG AAGGTCGAGT AAATCTGAAC CAATAGGGAG TAAAAAGTCT 
AAACCCTTAA TGAGATCTGA ACAAGAGAAA AGTACTCAAG AATCTGTTGA TGGCACAGTA 
GCCTTAGAAA ATAACCCACC TGGCTTGCTT AATCAGACAG AATGTGTGTT AGATAATCAG 
GTTCATCTCT CTGAATCTAC AGTGGAGTTT GAGGATACAA TGCTTGAATC AACAGTGGAG 
AATACTGTAT TAGAAAACAA TACTGTAGAA GAGAGAACCT TAGAAGTTAA CTTGGAATCC 
AAAGAAAACA CACCCCCAGC CATAATAACG GATCAGACAG TAAATGAGGA TAGTCATATT 
CAGGTAACTC CAAATCAGAA AACCCTTAGG CGATCTTTAA GGCGACGTTC AGAAACAGCA 
GAACCAACCA CTGATAGCCA AGATAAAGAA AATAATCATC AAAAAAGGGA AAGACGTAAA 
GAAGAAGAAA AGACTCCGCA GAAGAGTCCG TTACATGTAA AAGATGATGT TTTACCTAAG 
CAAAAATTGA TTTCTGAACA AAGTGTACAG GGAAACTTAG TTGACAAAGG AAATAATTTA 
CATGAGAAGG CTTTTGGGGA GACCAGTGCT AATGCTGAAA TTGATGAAAA TAGAAGAAAG 
CCAGATCTTG AGAATACTAA ATCTGAAGGA GATGGTGTGC CGGACATTGC AGATAAATCC 
TCTGAGAAAC CAGTAAGGGG ACGAACACGA TACCAAACAA GAAGAGCATC CCAGGGTTTA 
CTTTCCAGCA TTGAAAACTC AGAATCTGAT AGTTCTGAGG CAAAAGAAGA AGGTTCTAAA 
AAGAAAAGAT CTGGAAAATG GAAGAACAAA AGCAGTGACA ATGTTGACAT GGAAGATCAA 
GAAGAGAAAA TGGTAAAACA GGAATGTATA AAAATTGAAA ATCAGGCACA TGATTATAAA 
GGGAATCCTG AAGTAGACAA AGATATAAAA TCTCAAATCT GTGAAAAAGG AGATGAAAGT 
ACTACTGTAA GTCTTCAAGA TTCTACCGTA TCTCCAGATT TATTACAAGT TTATGATGAT 
GTATTGAATA CTTCTGAGGG AGAAAGTAAA AGTAACAAAG ATGCAGACCA TTCCTTTTCA 
AATCCTTCTG TACCAGAATC AAATCTAAGG ACTAGAAATG CCAGTAAGAG GTTACATAAG 
CGAGATTCTA TAGAAAATAG CATGGGGGAA TCCTCAAAAA TAGGGACATC AGACATTTCT 
TTGCTTTCTG AAAAAACTCT TCAGACAGTA GAATGCCAGC ACAAGAGAAG TAGGAGGGTG 
AGGCGATCTA AAGGTTGTGA TTGTTGTGGA GAAAAATCAC AGTCTCAGGA AAAGTCATTT 
ACTGGGTTAA AGGATACAGA AAATTACGAT GTAAAAGTCA GTGAAACAAA AAAGACAGAT 
GGGGAAACAC CTGTATCTAT TTCAGAAACT TCGAAAGCTG ATCTGTCCTC GGAGGTTAAA 
CTTTTAGATG AGCATCATAG TGTGAATTTT CATTTGGATC TCAAGGAAGA GAATGATACT 
ACTAATGATT CATTAATTAT ATCTGAAACT GAGTTAAAAG AAAATACTAC TCAAAGCTTT 
CTTCCTTCTG AAATGGTAAA ATTTAAAGAA ACTTCTCGTA GGGATTCTGA GGAAGCAATA 
TCTCTAGAAA CCCAGGAGCC ATCTAATAAG AAATGTAAAA CTGTTGACCC ACATTTAGAT 
GTTTCCAAAG ATATTTCACT GAAATGTTTT TCTTTGGAGA CTAAAGAAGA AAAGTCAGAG 
GCCACTTCAA AAGAGAAATT GAGTACAGAG AACATTGTTA TTGTTGAATC AAATGCAGAA 
TCAAATCCAG AAGTAGATGC TATGGAATTG AATGTAGAAA ATGATCAGTC TGAAGCTGGC 
TTCTCTGAAC AAAAGAATGT CAAAACTGGT ATTCCAGGGA AAGAAGTAAC AGAGGAAAAC 
AGTGAAAGAA TGGATACACC TGAAAAACTG AAAGCCAATG AAGCAGTAAC TGAGGAATTT 
AATTCAGCTA CTGATTATTC GGATACTCCG ACATCTGTAA AAGTGAATGC TCAGGTAGAG 
ATCTCTGAAC AAATAGCGGT TGGGGAGCTA GGTGGAGGAA GTGATGAATC TGATCCAGGC 
TCATCTGAAG AAATGAACGC TGAAATGAAA AATTGTGAAA AAACAGCGGT TTGTGAGGTG 
ACTACTGAAT CTGACCACGT CACTGAAACC GAAGACGAAG ATGTAACTAC CACGGCTTCT 
CTGCCTATAG AAGTTGAAAC AAAGGAATTA GATGTAGAAG TCAATAACTT TGTTCCCGAC 
ACACTTGAGA TGATCACTGA AGAAGGAAAG GTTGACCCTA ACAAAACTGA AACGAATACT 
GAACCTAATA AATTGGAGGA AACACAGTTA GATGACAATC AGATGGTAGT GGAAAGTAAT 
GAAACTTTAC CGGAAGTTCA CCATACTTCA GAGAAAGTAG AGGAGATAGC ACAACCTCTG 
ACATCTGAAA CAGATATTTC TGGACTAATA TCAGAAGACA GTAATACATC TCCTCAAAAA 
TCGAAGGATA TTGATCCTTT ACTCATGTCA GCAAACGAGA GTCCTAGTGG CATGCACACA 
CGTTGTGTGT GGTCTCCTTT GGCTTCTCCA TCCACCAGCA TTTTAAAGAG AGGACTAAAA 
AGACCCCAAG AAGATGAAAT CTCGTCACCT GTCCACAAGA TTGCAGAAAT GGCTAAGGAA 
TCTGTGCCAT GCCCAACAGA AAGTGTTTAC CCTGCTTTGG TGAACTGTGT AGCACCAGTT 
GAAATCATTT TACCTCAGAT TACATCCAAC ATGTGGGCAA GAGGACTGGG ACAGCTCATT 
AGAGCCAAGA ATATAAAAAC AATTGGCGAT TTGAGTACCC TTACAGCATC TGAAATAAAA 
ACTCTTCCTA TCCGTTCTCC AAAAGTGTCT AACGTTAAAA AGGCTCTCAG AATATATCAT 
GAGCAGCAGG TGAAGTCTCG TGGACTAGAA GAGATTCCAG TTTTTGATAT TTCTGAAAAA 
ACAATAAATG GAATGGAAAA TAAATCTATC TCTCCTGATG AAGAAAGACT TGCCTCAGAT 
TTAATTGATC CTGTTGCTTT AGAAGCTCCG TTATCTAAAA ATCTTGTGGC ACAGATTAGC 
GCACTTGCTC TTCAACTAGA TTCAGAAGAT CTCTATAATT ATTCAGGAAG CCAGCTATTT 
GAAATGCACG AGAAACTTGG TAGCATGGCA AACTCTATAA TTAAAAATCT GCAGTCACGT 
TGGAGGTCAC CAGCCCATGA AAATTCTTTT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RIF1 ( XM_010802077.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010800379.1 Bos taurus replication timing regulatory factor 1 (RIF1), transcript variant X6, mRNA.

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CloneID	OBa55068
Clone ID Related Accession (Same CDS sequence)	XM_010826493.2 , XM_010802077.3 , XM_010802077.2
Accession Version	XM_010802077.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7173bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	telomere-associated protein RIF1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103838.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010802077.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGCCG CGGATCAGAG CCCCCTGGCA CCGCTGTTGG AGACTTTGGA AGACCCTTCC 
GCCTCCCTTG GAGAGCAGAC CGACGCTTAC CTGACCCTGA CCAGTCGTAT GACTGGAGAA 
GATGGAAAAG AAATCATTAC AGAAATTGAG AAAAAACTTC CTCAGCTATT TAAAGTTTTA 
AAGACTCACA TTGCCAGTCA AAACTCAGAG CTCAGTAGTG CTGCTCTACA GGCCTTGGGG 
TTTTGCTTAT ATAATCCCAA AATTGCCTCT GGATTATCAG AGATAAATAT TCAAGAACTG 
CTTTCAAAAC TGAATGATAT TGTTAAAAGT TCAGATAAAA ATGTACGTAC TAGGGCACTT 
TGGGTGATAT CCAAGCAGAC GTTTCCCACT GAAGTCGTTG GCAAAGTAGT TTCTAGTATC 
ATTGACTCAC TAGAAATAGT ATTTAATAGA GGAGAGATGC TTTCTGCTGT TGTGGATTAT 
GAAGCATTAA ATGTCATTAT AAGACTAATT GAACAAGCCC CAGTTCAAAT GGGAGAAGAG 
TCAATTAGGT GGGCAAAATT GGTCATACCT TTACTGGTTC ATTCAGCCCA AAAAGTACAT 
TTGCGGGGAG CCACTGCTCT AGAGATGGGA ATGCCTTTGT TGCTTCAGAA ACAGCAAGAA 
ATAGCATCTA TCACAGAGCA GCTTATGACT ACCAAATTAC TTTCAGAACT CCAGAAACTG 
TTTATGAGTA AAAATGAGAC TTATGTGTTA AAATTGTGGC CTTTATTTGT CAAACTTCTT 
GGGAAGACCT TGCATCGAAG TGGGAGTTTC ATTAATTCTC TGTTACAGCT GGAAGAACTG 
GGATTTCGTA GTGGAGCACC CATGATTAAA AAAATAGCTT TTATTGCTTG GAAGAGTTTA 
ATAGATAATT TTGCTTTAAA TCCAGAAATA CTGTGTAGTG CAAAAAGACT GAAGTTGTTG 
ATGCAGCCTT TGAGTTCTAT CCATGTGAGA ACAGAAACTC TAGCGCTGAC AAAACTAGAA 
GTCTGGTGGT ATTTGTTGAT GAGACTAGGA CCTCATCTTC CTGCTAATTT TGAACAGGTT 
TGTGTGCCTC TGATTCAGAG CACAATAAGC ATTGATTCTA ATGCTTCACC TCAAGGGAGT 
TCATCTCGTG TAGCTACTAC TCCAGGTTTA AGCCCTATGA CCCCAGTACA CAAAGGTGCT 
TCTCCATATG GAACTCCTGT AACCCCTCGG ATGAACTTGA ATTCAAATTT TGGAATGGCC 
ACAATCCCCT CTATACAACT TTTGGGGCTT GAAATGTTGC TTCATTTTTT GTTGGGTCCA 
GAAGTCGTGA GTTTTGCTAA GCAAAACAAA CTTATACTGA GCCTAGAACC ACTTGAACAT 
CCATTAATCA GCAGCTCTTC TTTTTTCTCC AAACACTCAA ATACACTTAT CACTGCTGTT 
CATGATAGCT TTGTTGCAGT TGGAAAAGAT GCTTCTGATG TAGTTATTAA TGCTATTTGG 
AAGGAGTTAA TTAGCTTGGT GAAGTCAGTT ATTGAATCAG GTAACAAAAA AGAGAGACCA 
GGTTCTGAAG TTTTGACTCT CTTATTAAAA TCTTTGGAAA GCATAGTGAA TTCAGAAGTA 
TTTCCTGTAT TGAAAACACT GGTTCTCATG GAAATTACAA TTAAAGGACT TCCTCAGAAA 
GTATTAGGTT CTCCAGCATA TCAGGTTGCT AATATGGATA TTCTTAATGG AACCCCTGCT 
TTGTTCTTAA TTCAGTTAAC TTTCAATAAT AATCTATTGG AATGTGGTGT GGAAGACGAA 
AGGTTTTTTC TCAACCTGGA AACACTTGTG GGCTGTGTTC TTTCGGGTCC AACTTCACCA 
CTCGCTTTCA GTGACTCTGT CTTAAATGTT GTCAATCAAA ATGCAAAGCA GTTGGATAAC 
AAGGAGCATC TTTGGAGAAT GTGGAGTATT ATAGTCACCC CATTAACAGA ATTGATTAAT 
CAGACCAATG AAGTGAATCA AGGGGATGCC TTAGAACATA ATTTCAGTGC TATCTATGGT 
GCACTGATTT TGCCAATAAA CCATATTTTT CCAGCACAAA AAATTCCAGT GGTCACCATG 
AAGACCTTAC TTAAAACTTG GTCAGAATTA TATAGAGCAT TTGCTCGCTG TGCAGCTTTG 
GTGGCAACTG CGGAAGAGAA TTTATGCTGT GAGGAATTTT GTTCCAAGAT AATGTCCAGT 
TTGGAAGATG AAGGCCTTTC AAATTTATTG TTCTTGGATA GGATCACTCA TATTATTACT 
GTAATGGTTG ATTGCATCGA CTTTTCACCA TATAATATTA AATATCAACC CAAAACTAAA 
TCACCACAGA GACCTACAGA TTGGTCCAAA AAGAAGAAGG AACCTTTAGG GAAGTTGGCT 
TCTTTAATTA AACTTATTGT GAGAGTGATC TATTCTTTTC ACACACTGAG CCTCAAGGAA 
GTACATTCTG ATACTCTCCT CACTATTGGC AACTCAATCA CCAGCATTCT TTCCAATTTA 
CTTGGACATA TTTCTCTGCC TTCTATGATC CAGAAAATAT TTGCAGCTTT AACAAGACCT 
CTGGCATTAT TTTATGAAAA CTCAAAGCTT GATGAAGTTC CTAAAGTGTA TAGTTGTATG 
AACAACAAGT TAGAAAAGCT ACTAGGAGAT ATAATTGCTT GTCTACATGT CAGCTACACT 
GGAACTTACG ACAGTGAACT TCTTGAACAG ATCTCACCAT TATTATGCAT AATATTTTCA 
CACAAGAACA AACAGATAAG AAAACAAAGT GCTCAGTTCT GGAATGCCAC ATTTGCTAAA 
GTGACGATGT TGATTTATCC TGAGGAGTTA AAGCCAATAC TGAAACAAGC CAAACAAAAA 
TCTCTGCTTT TATTGCCTGG TTTGGAAAAT GTTGAAATTA TGGAGGAATC CAGTGGACCA 
TATTCAGATA TGACAGAAAA TTCCCAATTA AATATGAAGA TAAGTGGCAT GGAAAGAAAA 
TCAAGTGGAA AAAGAGATTC TTTTTTGGCA CAAACAAAGG ATACAAAAGA CAGTATGAAA 
CCACCAGCTA AATTGAAACT AGATTCTTCA ACTCCAAAAG CAAAGGGTGA AATGCCTTTG 
GAAGAAGAAA AGTCTACTGA CTTTGTGTTT ATACCTCCAG AAGTAAAAGA AACAAAGGAA 
AGAATACTAA CTGAACACCA GAAAGAAGTA CTCAAAACAA AGAGGTGTGA TATTCCTGCC 
ATGTATAATA ATCTGGATGT TTCACAAGAT ACTTTATTTT CTCAGTATAG TCAGGAAGAA 
TCTATGGAAA TTTCTACTTT AATTGAAAAA TCAAAAGAAG ACTCCAAGAT GATATTTAAG 
GAGGAACAAA AGGAGAATGA CATTGTCGTT CCTCAACATG TCATGGAAAA CTGCGGTATG 
GATGAACATC TTGAAAAGGC TTCCCTTTTG AATAATGAGT GTGGTTCTAT TGAGGAAACC 
AGTCCAAAAA TACTGAGCAG TAATAATGAT GCCAGAAAAA AAGCTTTAAT TGCTTCAAAT 
AAACCAACAG AATGTGCATC TACTACAGAA AATCCATCTG GTGTCAGCAG TAGCTCGGTT 
TCTATTGCCA CCATTTCTGG AGCTCCCTTA CAGCCTACAA GTCGAAGGCA ATCCTTTATT 
ACTTTGGAAA AGTTTGATGG TTCAGAAAAT AGACCTTTTA GTCCATCACC CTTGAATAAT 
ATGTCATCAA CTGTAATAGT GAAAAGTAAC CAGGAAGGCA TGGGTAAAAC AGATAATCCA 
TCAAAAGCAA AGAAGAGAGA AGTGGCTCTT TCCAAATCTG ACTCTGAAAA AATAACACAT 
GGGACTAAGA GATCAAGCCG AAGGTCGAGT AAATCTGAAC CAATAGGGAG TAAAAAGTCT 
AAACCCTTAA TGAGATCTGA ACAAGAGAAA AGTACTCAAG AATCTGTTGA TGGCACAGTA 
GCCTTAGAAA ATAACCCACC TGGCTTGCTT AATCAGACAG AATGTGTGTT AGATAATCAG 
GTTCATCTCT CTGAATCTAC AGTGGAGTTT GAGGATACAA TGCTTGAATC AACAGTGGAG 
AATACTGTAT TAGAAAACAA TACTGTAGAA GAGAGAACCT TAGAAGTTAA CTTGGAATCC 
AAAGAAAACA CACCCCCAGC CATAATAACG GATCAGACAG TAAATGAGGA TAGTCATATT 
CAGGTAACTC CAAATCAGAA AACCCTTAGG CGATCTTTAA GGCGACGTTC AGAAACAGCA 
GAACCAACCA CTGATAGCCA AGATAAAGAA AATAATCATC AAAAAAGGGA AAGACGTAAA 
GAAGAAGAAA AGACTCCGCA GAAGAGTCCG TTACATGTAA AAGATGATGT TTTACCTAAG 
CAAAAATTGA TTTCTGAACA AAGTGTACAG GGAAACTTAG TTGACAAAGG AAATAATTTA 
CATGAGAAGG CTTTTGGGGA GACCAGTGCT AATGCTGAAA TTGATGAAAA TAGAAGAAAG 
CCAGATCTTG AGAATACTAA ATCTGAAGGA GATGGTGTGC CGGACATTGC AGATAAATCC 
TCTGAGAAAC CAGTAAGGGG ACGAACACGA TACCAAACAA GAAGAGCATC CCAGGGTTTA 
CTTTCCAGCA TTGAAAACTC AGAATCTGAT AGTTCTGAGG CAAAAGAAGA AGGTTCTAAA 
AAGAAAAGAT CTGGAAAATG GAAGAACAAA AGCAGTGACA ATGTTGACAT GGAAGATCAA 
GAAGAGAAAA TGGTAAAACA GGAATGTATA AAAATTGAAA ATCAGGCACA TGATTATAAA 
GGGAATCCTG AAGTAGACAA AGATATAAAA TCTCAAATCT GTGAAAAAGG AGATGAAAGT 
ACTACTGTAA GTCTTCAAGA TTCTACCGTA TCTCCAGATT TATTACAAGT TTATGATGAT 
GTATTGAATA CTTCTGAGGG AGAAAGTAAA AGTAACAAAG ATGCAGACCA TTCCTTTTCA 
AATCCTTCTG TACCAGAATC AAATCTAAGG ACTAGAAATG CCAGTAAGAG GTTACATAAG 
CGAGATTCTA TAGAAAATAG CATGGGGGAA TCCTCAAAAA TAGGGACATC AGACATTTCT 
TTGCTTTCTG AAAAAACTCT TCAGACAGTA GAATGCCAGC ACAAGAGAAG TAGGAGGGTG 
AGGCGATCTA AAGGTTGTGA TTGTTGTGGA GAAAAATCAC AGTCTCAGGA AAAGTCATTT 
ACTGGGTTAA AGGATACAGA AAATTACGAT GTAAAAGTCA GTGAAACAAA AAAGACAGAT 
GGGGAAACAC CTGTATCTAT TTCAGAAACT TCGAAAGCTG ATCTGTCCTC GGAGGTTAAA 
CTTTTAGATG AGCATCATAG TGTGAATTTT CATTTGGATC TCAAGGAAGA GAATGATACT 
ACTAATGATT CATTAATTAT ATCTGAAACT GAGTTAAAAG AAAATACTAC TCAAAGCTTT 
CTTCCTTCTG AAATGGTAAA ATTTAAAGAA ACTTCTCGTA GGGATTCTGA GGAAGCAATA 
TCTCTAGAAA CCCAGGAGCC ATCTAATAAG AAATGTAAAA CTGTTGACCC ACATTTAGAT 
GTTTCCAAAG ATATTTCACT GAAATGTTTT TCTTTGGAGA CTAAAGAAGA AAAGTCAGAG 
GCCACTTCAA AAGAGAAATT GAGTACAGAG AACATTGTTA TTGTTGAATC AAATGCAGAA 
TCAAATCCAG AAGTAGATGC TATGGAATTG AATGTAGAAA ATGATCAGTC TGAAGCTGGC 
TTCTCTGAAC AAAAGAATGT CAAAACTGGT ATTCCAGGGA AAGAAGTAAC AGAGGAAAAC 
AGTGAAAGAA TGGATACACC TGAAAAACTG AAAGCCAATG AAGCAGTAAC TGAGGAATTT 
AATTCAGCTA CTGATTATTC GGATACTCCG ACATCTGTAA AAGTGAATGC TCAGGTAGAG 
ATCTCTGAAC AAATAGCGGT TGGGGAGCTA GGTGGAGGAA GTGATGAATC TGATCCAGGC 
TCATCTGAAG AAATGAACGC TGAAATGAAA AATTGTGAAA AAACAGCGGT TTGTGAGGTG 
ACTACTGAAT CTGACCACGT CACTGAAACC GAAGACGAAG ATGTAACTAC CACGGCTTCT 
CTGCCTATAG AAGTTGAAAC AAAGGAATTA GATGTAGAAG TCAATAACTT TGTTCCCGAC 
ACACTTGAGA TGATCACTGA AGAAGGAAAG GTTGACCCTA ACAAAACTGA AACGAATACT 
GAACCTAATA AATTGGAGGA AACACAGTTA GATGACAATC AGATGGTAGT GGAAAGTAAT 
GAAACTTTAC CGGAAGTTCA CCATACTTCA GAGAAAGTAG AGGAGATAGC ACAACCTCTG 
ACATCTGAAA CAGATATTTC TGGACTAATA TCAGAAGACA GTAATACATC TCCTCAAAAA 
TCGAAGGATA TTGATCCTTT ACTCATGTCA GCAAACGAGA GTCCTAGTGG CATGCACACA 
CGTTGTGTGT GGTCTCCTTT GGCTTCTCCA TCCACCAGCA TTTTAAAGAG AGGACTAAAA 
AGACCCCAAG AAGATGAAAT CTCGTCACCT GTCCACAAGA TTGCAGAAAT GGCTAAGGAA 
TCTGTGCCAT GCCCAACAGA AAGTGTTTAC CCTGCTTTGG TGAACTGTGT AGCACCAGTT 
GAAATCATTT TACCTCAGAT TACATCCAAC ATGTGGGCAA GAGGACTGGG ACAGCTCATT 
AGAGCCAAGA ATATAAAAAC AATTGGCGAT TTGAGTACCC TTACAGCATC TGAAATAAAA 
ACTCTTCCTA TCCGTTCTCC AAAAGTGTCT AACGTTAAAA AGGCTCTCAG AATATATCAT 
GAGCAGCAGG TGAAGTCTCG TGGACTAGAA GAGATTCCAG TTTTTGATAT TTCTGAAAAA 
ACAATAAATG GAATGGAAAA TAAATCTATC TCTCCTGATG AAGAAAGACT TGCCTCAGAT 
TTAATTGATC CTGTTGCTTT AGAAGCTCCG TTATCTAAAA ATCTTGTGGC ACAGATTAGC 
GCACTTGCTC TTCAACTAGA TTCAGAAGAT CTCTATAATT ATTCAGGAAG CCAGCTATTT 
GAAATGCACG AGAAACTTGG TAGCATGGCA AACTCTATAA TTAAAAATCT GCAGTCACGT 
TGGAGGTCAC CAGCCCATGA AAATTCTTTT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010800379.1
CDS	146..7318
Translation	MTAADQSPLAPLLETLEDPSASLGEQTDAYLTLTSRMTGEDGKEIITEIEKKLPQLFKVLKTHIASQNSELSSAALQALGFCLYNPKIASGLSEINIQELLSKLNDIVKSSDKNVRTRALWVISKQTFPTEVVGKVVSSIIDSLEIVFNRGEMLSAVVDYEALNVIIRLIEQAPVQMGEESIRWAKLVIPLLVHSAQKVHLRGATALEMGMPLLLQKQQEIASITEQLMTTKLLSELQKLFMSKNETYVLKLWPLFVKLLGKTLHRSGSFINSLLQLEELGFRSGAPMIKKIAFIAWKSLIDNFALNPEILCSAKRLKLLMQPLSSIHVRTETLALTKLEVWWYLLMRLGPHLPANFEQVCVPLIQSTISIDSNASPQGSSSRVATTPGLSPMTPVHKGASPYGTPVTPRMNLNSNFGMATIPSIQLLGLEMLLHFLLGPEVVSFAKQNKLILSLEPLEHPLISSSSFFSKHSNTLITAVHDSFVAVGKDASDVVINAIWKELISLVKSVIESGNKKERPGSEVLTLLLKSLESIVNSEVFPVLKTLVLMEITIKGLPQKVLGSPAYQVANMDILNGTPALFLIQLTFNNNLLECGVEDERFFLNLETLVGCVLSGPTSPLAFSDSVLNVVNQNAKQLDNKEHLWRMWSIIVTPLTELINQTNEVNQGDALEHNFSAIYGALILPINHIFPAQKIPVVTMKTLLKTWSELYRAFARCAALVATAEENLCCEEFCSKIMSSLEDEGLSNLLFLDRITHIITVMVDCIDFSPYNIKYQPKTKSPQRPTDWSKKKKEPLGKLASLIKLIVRVIYSFHTLSLKEVHSDTLLTIGNSITSILSNLLGHISLPSMIQKIFAALTRPLALFYENSKLDEVPKVYSCMNNKLEKLLGDIIACLHVSYTGTYDSELLEQISPLLCIIFSHKNKQIRKQSAQFWNATFAKVTMLIYPEELKPILKQAKQKSLLLLPGLENVEIMEESSGPYSDMTENSQLNMKISGMERKSSGKRDSFLAQTKDTKDSMKPPAKLKLDSSTPKAKGEMPLEEEKSTDFVFIPPEVKETKERILTEHQKEVLKTKRCDIPAMYNNLDVSQDTLFSQYSQEESMEISTLIEKSKEDSKMIFKEEQKENDIVVPQHVMENCGMDEHLEKASLLNNECGSIEETSPKILSSNNDARKKALIASNKPTECASTTENPSGVSSSSVSIATISGAPLQPTSRRQSFITLEKFDGSENRPFSPSPLNNMSSTVIVKSNQEGMGKTDNPSKAKKREVALSKSDSEKITHGTKRSSRRSSKSEPIGSKKSKPLMRSEQEKSTQESVDGTVALENNPPGLLNQTECVLDNQVHLSESTVEFEDTMLESTVENTVLENNTVEERTLEVNLESKENTPPAIITDQTVNEDSHIQVTPNQKTLRRSLRRRSETAEPTTDSQDKENNHQKRERRKEEEKTPQKSPLHVKDDVLPKQKLISEQSVQGNLVDKGNNLHEKAFGETSANAEIDENRRKPDLENTKSEGDGVPDIADKSSEKPVRGRTRYQTRRASQGLLSSIENSESDSSEAKEEGSKKKRSGKWKNKSSDNVDMEDQEEKMVKQECIKIENQAHDYKGNPEVDKDIKSQICEKGDESTTVSLQDSTVSPDLLQVYDDVLNTSEGESKSNKDADHSFSNPSVPESNLRTRNASKRLHKRDSIENSMGESSKIGTSDISLLSEKTLQTVECQHKRSRRVRRSKGCDCCGEKSQSQEKSFTGLKDTENYDVKVSETKKTDGETPVSISETSKADLSSEVKLLDEHHSVNFHLDLKEENDTTNDSLIISETELKENTTQSFLPSEMVKFKETSRRDSEEAISLETQEPSNKKCKTVDPHLDVSKDISLKCFSLETKEEKSEATSKEKLSTENIVIVESNAESNPEVDAMELNVENDQSEAGFSEQKNVKTGIPGKEVTEENSERMDTPEKLKANEAVTEEFNSATDYSDTPTSVKVNAQVEISEQIAVGELGGGSDESDPGSSEEMNAEMKNCEKTAVCEVTTESDHVTETEDEDVTTTASLPIEVETKELDVEVNNFVPDTLEMITEEGKVDPNKTETNTEPNKLEETQLDDNQMVVESNETLPEVHHTSEKVEEIAQPLTSETDISGLISEDSNTSPQKSKDIDPLLMSANESPSGMHTRCVWSPLASPSTSILKRGLKRPQEDEISSPVHKIAEMAKESVPCPTESVYPALVNCVAPVEIILPQITSNMWARGLGQLIRAKNIKTIGDLSTLTASEIKTLPIRSPKVSNVKKALRIYHEQQVKSRGLEEIPVFDISEKTINGMENKSISPDEERLASDLIDPVALEAPLSKNLVAQISALALQLDSEDLYNYSGSQLFEMHEKLGSMANSIIKNLQSRWRSPAHENSF

Target ORF information:

RefSeq Version	XM_010802077.2
Organism	Bos taurus(cattle)
Definition	Bos taurus replication timing regulatory factor 1 (RIF1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010802077.2

ORF Insert Sequence:

ATGACGGCCG CGGATCAGAG CCCCCTGGCA CCGCTGTTGG AGACTTTGGA AGACCCTTCC 
GCCTCCCTTG GAGAGCAGAC CGACGCTTAC CTGACCCTGA CCAGTCGTAT GACTGGAGAA 
GATGGAAAAG AAATCATTAC AGAAATTGAG AAAAAACTTC CTCAGCTATT TAAAGTTTTA 
AAGACTCACA TTGCCAGTCA AAACTCAGAG CTCAGTAGTG CTGCTCTACA GGCCTTGGGG 
TTTTGCTTAT ATAATCCCAA AATTGCCTCT GGATTATCAG AGATAAATAT TCAAGAACTG 
CTTTCAAAAC TGAATGATAT TGTTAAAAGT TCAGATAAAA ATGTACGTAC TAGGGCACTT 
TGGGTGATAT CCAAGCAGAC GTTTCCCACT GAAGTCGTTG GCAAAGTAGT TTCTAGTATC 
ATTGACTCAC TAGAAATAGT ATTTAATAGA GGAGAGATGC TTTCTGCTGT TGTGGATTAT 
GAAGCATTAA ATGTCATTAT AAGACTAATT GAACAAGCCC CAGTTCAAAT GGGAGAAGAG 
TCAATTAGGT GGGCAAAATT GGTCATACCT TTACTGGTTC ATTCAGCCCA AAAAGTACAT 
TTGCGGGGAG CCACTGCTCT AGAGATGGGA ATGCCTTTGT TGCTTCAGAA ACAGCAAGAA 
ATAGCATCTA TCACAGAGCA GCTTATGACT ACCAAATTAC TTTCAGAACT CCAGAAACTG 
TTTATGAGTA AAAATGAGAC TTATGTGTTA AAATTGTGGC CTTTATTTGT CAAACTTCTT 
GGGAAGACCT TGCATCGAAG TGGGAGTTTC ATTAATTCTC TGTTACAGCT GGAAGAACTG 
GGATTTCGTA GTGGAGCACC CATGATTAAA AAAATAGCTT TTATTGCTTG GAAGAGTTTA 
ATAGATAATT TTGCTTTAAA TCCAGAAATA CTGTGTAGTG CAAAAAGACT GAAGTTGTTG 
ATGCAGCCTT TGAGTTCTAT CCATGTGAGA ACAGAAACTC TAGCGCTGAC AAAACTAGAA 
GTCTGGTGGT ATTTGTTGAT GAGACTAGGA CCTCATCTTC CTGCTAATTT TGAACAGGTT 
TGTGTGCCTC TGATTCAGAG CACAATAAGC ATTGATTCTA ATGCTTCACC TCAAGGGAGT 
TCATCTCGTG TAGCTACTAC TCCAGGTTTA AGCCCTATGA CCCCAGTACA CAAAGGTGCT 
TCTCCATATG GAACTCCTGT AACCCCTCGG ATGAACTTGA ATTCAAATTT TGGAATGGCC 
ACAATCCCCT CTATACAACT TTTGGGGCTT GAAATGTTGC TTCATTTTTT GTTGGGTCCA 
GAAGTCGTGA GTTTTGCTAA GCAAAACAAA CTTATACTGA GCCTAGAACC ACTTGAACAT 
CCATTAATCA GCAGCTCTTC TTTTTTCTCC AAACACTCAA ATACACTTAT CACTGCTGTT 
CATGATAGCT TTGTTGCAGT TGGAAAAGAT GCTTCTGATG TAGTTATTAA TGCTATTTGG 
AAGGAGTTAA TTAGCTTGGT GAAGTCAGTT ATTGAATCAG GTAACAAAAA AGAGAGACCA 
GGTTCTGAAG TTTTGACTCT CTTATTAAAA TCTTTGGAAA GCATAGTGAA TTCAGAAGTA 
TTTCCTGTAT TGAAAACACT GGTTCTCATG GAAATTACAA TTAAAGGACT TCCTCAGAAA 
GTATTAGGTT CTCCAGCATA TCAGGTTGCT AATATGGATA TTCTTAATGG AACCCCTGCT 
TTGTTCTTAA TTCAGTTAAC TTTCAATAAT AATCTATTGG AATGTGGTGT GGAAGACGAA 
AGGTTTTTTC TCAACCTGGA AACACTTGTG GGCTGTGTTC TTTCGGGTCC AACTTCACCA 
CTCGCTTTCA GTGACTCTGT CTTAAATGTT GTCAATCAAA ATGCAAAGCA GTTGGATAAC 
AAGGAGCATC TTTGGAGAAT GTGGAGTATT ATAGTCACCC CATTAACAGA ATTGATTAAT 
CAGACCAATG AAGTGAATCA AGGGGATGCC TTAGAACATA ATTTCAGTGC TATCTATGGT 
GCACTGATTT TGCCAATAAA CCATATTTTT CCAGCACAAA AAATTCCAGT GGTCACCATG 
AAGACCTTAC TTAAAACTTG GTCAGAATTA TATAGAGCAT TTGCTCGCTG TGCAGCTTTG 
GTGGCAACTG CGGAAGAGAA TTTATGCTGT GAGGAATTTT GTTCCAAGAT AATGTCCAGT 
TTGGAAGATG AAGGCCTTTC AAATTTATTG TTCTTGGATA GGATCACTCA TATTATTACT 
GTAATGGTTG ATTGCATCGA CTTTTCACCA TATAATATTA AATATCAACC CAAAACTAAA 
TCACCACAGA GACCTACAGA TTGGTCCAAA AAGAAGAAGG AACCTTTAGG GAAGTTGGCT 
TCTTTAATTA AACTTATTGT GAGAGTGATC TATTCTTTTC ACACACTGAG CCTCAAGGAA 
GTACATTCTG ATACTCTCCT CACTATTGGC AACTCAATCA CCAGCATTCT TTCCAATTTA 
CTTGGACATA TTTCTCTGCC TTCTATGATC CAGAAAATAT TTGCAGCTTT AACAAGACCT 
CTGGCATTAT TTTATGAAAA CTCAAAGCTT GATGAAGTTC CTAAAGTGTA TAGTTGTATG 
AACAACAAGT TAGAAAAGCT ACTAGGAGAT ATAATTGCTT GTCTACATGT CAGCTACACT 
GGAACTTACG ACAGTGAACT TCTTGAACAG ATCTCACCAT TATTATGCAT AATATTTTCA 
CACAAGAACA AACAGATAAG AAAACAAAGT GCTCAGTTCT GGAATGCCAC ATTTGCTAAA 
GTGACGATGT TGATTTATCC TGAGGAGTTA AAGCCAATAC TGAAACAAGC CAAACAAAAA 
TCTCTGCTTT TATTGCCTGG TTTGGAAAAT GTTGAAATTA TGGAGGAATC CAGTGGACCA 
TATTCAGATA TGACAGAAAA TTCCCAATTA AATATGAAGA TAAGTGGCAT GGAAAGAAAA 
TCAAGTGGAA AAAGAGATTC TTTTTTGGCA CAAACAAAGG ATACAAAAGA CAGTATGAAA 
CCACCAGCTA AATTGAAACT AGATTCTTCA ACTCCAAAAG CAAAGGGTGA AATGCCTTTG 
GAAGAAGAAA AGTCTACTGA CTTTGTGTTT ATACCTCCAG AAGTAAAAGA AACAAAGGAA 
AGAATACTAA CTGAACACCA GAAAGAAGTA CTCAAAACAA AGAGGTGTGA TATTCCTGCC 
ATGTATAATA ATCTGGATGT TTCACAAGAT ACTTTATTTT CTCAGTATAG TCAGGAAGAA 
TCTATGGAAA TTTCTACTTT AATTGAAAAA TCAAAAGAAG ACTCCAAGAT GATATTTAAG 
GAGGAACAAA AGGAGAATGA CATTGTCGTT CCTCAACATG TCATGGAAAA CTGCGGTATG 
GATGAACATC TTGAAAAGGC TTCCCTTTTG AATAATGAGT GTGGTTCTAT TGAGGAAACC 
AGTCCAAAAA TACTGAGCAG TAATAATGAT GCCAGAAAAA AAGCTTTAAT TGCTTCAAAT 
AAACCAACAG AATGTGCATC TACTACAGAA AATCCATCTG GTGTCAGCAG TAGCTCGGTT 
TCTATTGCCA CCATTTCTGG AGCTCCCTTA CAGCCTACAA GTCGAAGGCA ATCCTTTATT 
ACTTTGGAAA AGTTTGATGG TTCAGAAAAT AGACCTTTTA GTCCATCACC CTTGAATAAT 
ATGTCATCAA CTGTAATAGT GAAAAGTAAC CAGGAAGGCA TGGGTAAAAC AGATAATCCA 
TCAAAAGCAA AGAAGAGAGA AGTGGCTCTT TCCAAATCTG ACTCTGAAAA AATAACACAT 
GGGACTAAGA GATCAAGCCG AAGGTCGAGT AAATCTGAAC CAATAGGGAG TAAAAAGTCT 
AAACCCTTAA TGAGATCTGA ACAAGAGAAA AGTACTCAAG AATCTGTTGA TGGCACAGTA 
GCCTTAGAAA ATAACCCACC TGGCTTGCTT AATCAGACAG AATGTGTGTT AGATAATCAG 
GTTCATCTCT CTGAATCTAC AGTGGAGTTT GAGGATACAA TGCTTGAATC AACAGTGGAG 
AATACTGTAT TAGAAAACAA TACTGTAGAA GAGAGAACCT TAGAAGTTAA CTTGGAATCC 
AAAGAAAACA CACCCCCAGC CATAATAACG GATCAGACAG TAAATGAGGA TAGTCATATT 
CAGGTAACTC CAAATCAGAA AACCCTTAGG CGATCTTTAA GGCGACGTTC AGAAACAGCA 
GAACCAACCA CTGATAGCCA AGATAAAGAA AATAATCATC AAAAAAGGGA AAGACGTAAA 
GAAGAAGAAA AGACTCCGCA GAAGAGTCCG TTACATGTAA AAGATGATGT TTTACCTAAG 
CAAAAATTGA TTTCTGAACA AAGTGTACAG GGAAACTTAG TTGACAAAGG AAATAATTTA 
CATGAGAAGG CTTTTGGGGA GACCAGTGCT AATGCTGAAA TTGATGAAAA TAGAAGAAAG 
CCAGATCTTG AGAATACTAA ATCTGAAGGA GATGGTGTGC CGGACATTGC AGATAAATCC 
TCTGAGAAAC CAGTAAGGGG ACGAACACGA TACCAAACAA GAAGAGCATC CCAGGGTTTA 
CTTTCCAGCA TTGAAAACTC AGAATCTGAT AGTTCTGAGG CAAAAGAAGA AGGTTCTAAA 
AAGAAAAGAT CTGGAAAATG GAAGAACAAA AGCAGTGACA ATGTTGACAT GGAAGATCAA 
GAAGAGAAAA TGGTAAAACA GGAATGTATA AAAATTGAAA ATCAGGCACA TGATTATAAA 
GGGAATCCTG AAGTAGACAA AGATATAAAA TCTCAAATCT GTGAAAAAGG AGATGAAAGT 
ACTACTGTAA GTCTTCAAGA TTCTACCGTA TCTCCAGATT TATTACAAGT TTATGATGAT 
GTATTGAATA CTTCTGAGGG AGAAAGTAAA AGTAACAAAG ATGCAGACCA TTCCTTTTCA 
AATCCTTCTG TACCAGAATC AAATCTAAGG ACTAGAAATG CCAGTAAGAG GTTACATAAG 
CGAGATTCTA TAGAAAATAG CATGGGGGAA TCCTCAAAAA TAGGGACATC AGACATTTCT 
TTGCTTTCTG AAAAAACTCT TCAGACAGTA GAATGCCAGC ACAAGAGAAG TAGGAGGGTG 
AGGCGATCTA AAGGTTGTGA TTGTTGTGGA GAAAAATCAC AGTCTCAGGA AAAGTCATTT 
ACTGGGTTAA AGGATACAGA AAATTACGAT GTAAAAGTCA GTGAAACAAA AAAGACAGAT 
GGGGAAACAC CTGTATCTAT TTCAGAAACT TCGAAAGCTG ATCTGTCCTC GGAGGTTAAA 
CTTTTAGATG AGCATCATAG TGTGAATTTT CATTTGGATC TCAAGGAAGA GAATGATACT 
ACTAATGATT CATTAATTAT ATCTGAAACT GAGTTAAAAG AAAATACTAC TCAAAGCTTT 
CTTCCTTCTG AAATGGTAAA ATTTAAAGAA ACTTCTCGTA GGGATTCTGA GGAAGCAATA 
TCTCTAGAAA CCCAGGAGCC ATCTAATAAG AAATGTAAAA CTGTTGACCC ACATTTAGAT 
GTTTCCAAAG ATATTTCACT GAAATGTTTT TCTTTGGAGA CTAAAGAAGA AAAGTCAGAG 
GCCACTTCAA AAGAGAAATT GAGTACAGAG AACATTGTTA TTGTTGAATC AAATGCAGAA 
TCAAATCCAG AAGTAGATGC TATGGAATTG AATGTAGAAA ATGATCAGTC TGAAGCTGGC 
TTCTCTGAAC AAAAGAATGT CAAAACTGGT ATTCCAGGGA AAGAAGTAAC AGAGGAAAAC 
AGTGAAAGAA TGGATACACC TGAAAAACTG AAAGCCAATG AAGCAGTAAC TGAGGAATTT 
AATTCAGCTA CTGATTATTC GGATACTCCG ACATCTGTAA AAGTGAATGC TCAGGTAGAG 
ATCTCTGAAC AAATAGCGGT TGGGGAGCTA GGTGGAGGAA GTGATGAATC TGATCCAGGC 
TCATCTGAAG AAATGAACGC TGAAATGAAA AATTGTGAAA AAACAGCGGT TTGTGAGGTG 
ACTACTGAAT CTGACCACGT CACTGAAACC GAAGACGAAG ATGTAACTAC CACGGCTTCT 
CTGCCTATAG AAGTTGAAAC AAAGGAATTA GATGTAGAAG TCAATAACTT TGTTCCCGAC 
ACACTTGAGA TGATCACTGA AGAAGGAAAG GTTGACCCTA ACAAAACTGA AACGAATACT 
GAACCTAATA AATTGGAGGA AACACAGTTA GATGACAATC AGATGGTAGT GGAAAGTAAT 
GAAACTTTAC CGGAAGTTCA CCATACTTCA GAGAAAGTAG AGGAGATAGC ACAACCTCTG 
ACATCTGAAA CAGATATTTC TGGACTAATA TCAGAAGACA GTAATACATC TCCTCAAAAA 
TCGAAGGATA TTGATCCTTT ACTCATGTCA GCAAACGAGA GTCCTAGTGG CATGCACACA 
CGTTGTGTGT GGTCTCCTTT GGCTTCTCCA TCCACCAGCA TTTTAAAGAG AGGACTAAAA 
AGACCCCAAG AAGATGAAAT CTCGTCACCT GTCCACAAGA TTGCAGAAAT GGCTAAGGAA 
TCTGTGCCAT GCCCAACAGA AAGTGTTTAC CCTGCTTTGG TGAACTGTGT AGCACCAGTT 
GAAATCATTT TACCTCAGAT TACATCCAAC ATGTGGGCAA GAGGACTGGG ACAGCTCATT 
AGAGCCAAGA ATATAAAAAC AATTGGCGAT TTGAGTACCC TTACAGCATC TGAAATAAAA 
ACTCTTCCTA TCCGTTCTCC AAAAGTGTCT AACGTTAAAA AGGCTCTCAG AATATATCAT 
GAGCAGCAGG TGAAGTCTCG TGGACTAGAA GAGATTCCAG TTTTTGATAT TTCTGAAAAA 
ACAATAAATG GAATGGAAAA TAAATCTATC TCTCCTGATG AAGAAAGACT TGCCTCAGAT 
TTAATTGATC CTGTTGCTTT AGAAGCTCCG TTATCTAAAA ATCTTGTGGC ACAGATTAGC 
GCACTTGCTC TTCAACTAGA TTCAGAAGAT CTCTATAATT ATTCAGGAAG CCAGCTATTT 
GAAATGCACG AGAAACTTGG TAGCATGGCA AACTCTATAA TTAAAAATCT GCAGTCACGT 
TGGAGGTCAC CAGCCCATGA AAATTCTTTT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	RIF1
Entrez Gene ID	541247
Full Name	replication timing regulatory factor 1
General protein information	Preferred Names replication timing regulatory factor 1 Names telomere-associated protein RIF1 RAP1 interacting factor homolog
Gene Type	protein-coding
Organism	Bos taurus(cattle)

Pan troglodytes (chimpanzee)	RIF1	XP_001137674.1
Homo sapiens (human)	RIF1	NP_060621.3
Canis lupus familiaris (dog)	RIF1	XP_003639755.1
Bos taurus (cattle)	RIF1	XP_002685405.1
Rattus norvegicus (Norway rat)	Rif1	XP_006234257.1
Mus musculus (house mouse)	Rif1	NP_780447.4
Gallus gallus (chicken)	RIF1	XP_422162.3
	LOC694817	XP_002808045.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

RIF1 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

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Target ORF information:

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Target ORF information:

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Target ORF information:

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Target ORF information:

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Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

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