EML4 cDNA ORF clone, Bos taurus(cattle)

The following EML4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EML4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa39555 NM_001193054.1
Latest version!
Bos taurus echinoderm microtubule associated protein like 4 (EML4), mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa238865 NM_001193054.2
Latest version!
Bos taurus EMAP like 4 (EML4), mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBa220579 XM_024999054.1
Latest version!
Bos taurus echinoderm microtubule associated protein like 4 (EML4), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBa220579 XM_024999053.1
Latest version!
Bos taurus echinoderm microtubule associated protein like 4 (EML4), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBa220580 XM_024999055.1
Latest version!
Bos taurus echinoderm microtubule associated protein like 4 (EML4), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBa220581 XM_024999056.1
Latest version!
Bos taurus echinoderm microtubule associated protein like 4 (EML4), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBa54582 XM_024999051.1
Latest version!
Bos taurus echinoderm microtubule associated protein like 4 (EML4), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OBa54583 XM_024999052.1
Latest version!
Bos taurus echinoderm microtubule associated protein like 4 (EML4), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OBa91870 XM_015473450.1
Latest version!
Bos taurus echinoderm microtubule associated protein like 4 (EML4), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OBa91871 XM_015473451.1
Latest version!
Bos taurus echinoderm microtubule associated protein like 4 (EML4), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OBa91872 XM_015473452.1
Latest version!
Bos taurus echinoderm microtubule associated protein like 4 (EML4), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OBa220578 XM_005212656.2
Latest version!
Bos taurus echinoderm microtubule associated protein like 4 (EML4), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa54581 XM_024999050.1
Latest version!
Bos taurus echinoderm microtubule associated protein like 4 (EML4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OBa91868 XM_015473448.1
Latest version!
Bos taurus echinoderm microtubule associated protein like 4 (EML4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OBa91869 XM_015473449.1
Latest version!
Bos taurus echinoderm microtubule associated protein like 4 (EML4), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OBa23952 XM_005212656.1
Latest version!
Bos taurus echinoderm microtubule associated protein like 4 (EML4), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa39555
    Clone ID Related Accession (Same CDS sequence) NM_001193054.1
    Accession Version NM_001193054.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2988bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-22
    Organism Bos taurus(cattle)
    Product echinoderm microtubule-associated protein-like 4
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from DAAA02030668.1. On Jul 17, 2010 this sequence version replaced XM_866635.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN02822088, SAMN02822089 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGAAGACA GCCTGCCTAG TCTCGCTTCA GTATGTGCTT CCTCTACACT TTCAAACTTC 
    TGTACTGATG ATAGTATTTC CGCTGCAAGT ACTTCTGATG TTCAGGATCG CCTCTCAGCC
    CTTGAGTTAC GAGTTCAACA GCAAGAAGAT GAAATCACAG TGCTAAAGGC AGCTTTGGCT
    GATGTTTTGA GGCGTCTTGC CATCTCTGAA GATCATGTAG CCTCGGTGAA AAAATCAGTC
    CCAAGTAAAG GCCAGCCAGG CCTTCGAGAA GCTATTTCTA TGTCCTGTAT AACCAACGGG
    AGTGGTACAA ACAGAAAACC AAGTCACACC AGCTCTGTCT CAATCGCAAG AAAAGAAACT
    CTTTCATCTG CTGCTAAAAG TGGTACAGAA AAAAAGAAAG AAAAACCACA AGGACAGAGA
    GAGAAAAAAG AGGACTCTCA CTCTAATGAT CAAAGTCCAC AAATTCGAGC ATCACCTTCT
    CCCCAGCCCT CTTCACAGCC TCTCCAAATA CACAGGCAAA CTCAAGAAAG CAAGAATTCT
    ACTCCCACCA AAAGCATAAA ACGACCATCA ACAGCTGAAA AATCACATAA TTCATGGGAA
    AATTCAGACG ATAGCCGTAA TAAATTATTG AAGGCAGTTT CAACATCAAA ATTAATATCA
    AAAGTTATAA AAAATACAGA CAAGCATAAA GATGTCATCA TCAACCAAGA AGGAGAATAC
    ATTAAAATGT TTATGCGAGG TCGACCAATT ACAATGTTCA TTCCTTCTGA TGTTGAGAAC
    TATGATGACA TCAGAACAGA ACTGCCTCCT GAGAAGCTCA AACTGGAGTG GGTATACGGT
    TATCGAGGAA AGGATTGTCG AGCTAATGTT TACCTTCTTC CTACCGGGGA AATTGTTTAT
    TTCATAGCAT CCGTAGTAGT ACTGTTTAAT TATGAAGAGA GAACTCAACG GCACTACCTG
    GGTCATACAG ACTGTGTAAA ATGCCTTGCT ATACATCCTG ACAAAATTAG GATTGCAACT
    GGACAGATAG CTGGAGTGGA TAAGGATGGA AGGCCCCTGC AACCCCATGT CAGAGTGTGG
    GATTCAGTTA GTCTTTCTAC ACTGCAAATT ATTGGACTTG GAACTTTTGA ACGTGGAGTA
    GGATGCCTGG ATTTTTCAAA AGCAGATTCA GGTGTTCATT TATGTGTTAT CGATGACTCC
    AATGAGCATA TGCTTACTGT GTGGGATTGG CAGAAAAAAT CAAAAGGAGC AGAAATAAAG
    ACAACAAATG AAGTTGTTTT GGCTGTGGGG TTCCATCCAA CAGATGCAAA TATCATAATA
    ACATGTGGTA AATCACATAT TTTTTTCTGG ACCTGGAGTG GCAATTCACT AACAAAAAAA
    CAAGGAGTTT TTGGGAAATA CGAAAAACCA AAATTTGTGC AGTGTTTAGC TTTCTTGGGA
    AATGGAGATG TTCTTGCTGG AGACTCAGGT GGAGTCATAC TCATATGGAG CAAAACTACT
    GTAGAGCCCA CACCTGGGAA AGGGCCTAAA GGTGTTTATC AGATCAGCAA ACAAATCAAA
    GCTCATGATG GCAGTGTGTT CACACTTTGT CAGATGAGAA ATGGGATGTT ATTAACTGGA
    GGTGGGAAAG ACAGAAAAAT AATTCTGTGG GATCATGATC TGAATCCTGA AAGAGAAATA
    GAGGTTCCTG ATCAGTATGG AACAATCAGA GCTGTAGCAG AAGGAAAGGC AGATCAATTT
    TTGGTCGGCA CATCACGAAA CTTTATTTTA CGAGGAACAT TTAATGATGG CTTCCAAATA
    GAAGTACAGG GTCATACAGA TGAGCTTTGG GGTCTTGCCA CACATCCCTT CAAAGACTTG
    CTCTTGACTT GTGCTCAGGA CAGACAGGTG TGCATGTGGA ACTCAGTAGA ACACAGACTG
    GAATGGACCA GGCTTCTAGA TGAACCAGGA CACTGTGCAG ATTTTCATCC AAGTGGCACA
    GTGGTGGCCA TAGGAACGCA CTCAGGCAGG TGGTTTGTTC TGGATGCAGA AACCAGAGAT
    CTAGTTTCTA TCCACACAGA CGGAAATGAG CAGCTTTCGG TGATGCGCTA CTCAATAGAT
    GGCACCCTCC TGGCTGTAGG ATCTCATGAC AACTTCATTT ACCTCTATGC CGTCTCAGAA
    AATGGAAGAA AGTATAGCAG ACATGGAAAG TGCACTGGAC ATTCCAGCTA TATCACACAC
    CTTGACTGGT CCCCAGACAA CAAGTATATA ATGTCTAACT CGGGAGACTA TGAGATCCTG
    TACTGGGACA TTGAACATGG CTGCAAACTA ATGAGGAATC GATCAGAATG TAAGGACATC
    AATTGGACGA CATACACCTG TGTGCTAGGC TTCCAAGTCT TTGGTGTCTG GCCAGAAGGA
    TCTGATGGGA CAGATATCAA CGCGCTGGTA CGATCCCACA ACAGAAAGGT GATCGCTGTT
    GCTGATGACT TTTGCAAAGT CCATCTGTTT CAGTATCCCT GCTCCAAAGC AAAGGCTCCC
    AGTCACAAGT ACAGTGCCCA CAGCAGCCAT GTCACCAATG TCAGTTTTAC TCATAATGAC
    AGTCACCTGA TTTCAACTGG TGGAAAAGAC ATGAGCATCA TTCAGTGGAA ACTTGTGGAA
    AAGTTACCTT TGCCTCAAAA TGAGACTCTA GCGGATCCTA CACTCACCAA AGCCCCTCCC
    TCTTCCATCG AGAGTGTGGT GCAGTCTGAT ACCCCCACTC CGCCTCCTTC CCAGCCCTTA
    AATGAGACAG CTGAAGAAGA AAGTCGGATA AGCAGTTCTC CCACACTTCT GGAGAACAGC
    TTGGAACAGA CCGTGGAGCC CAGCGAAGAC CACAGTGAGG ATCAGAGTGA GGGGGGCAGC
    GAAGACCTCG GTGAGCCTGT TTATGAAGAG CTAGCCAATG AGCTAAGCGA GGAGCAGAGC
    AAAGCCGCCC TTCCCGGGGA CCAGCAAGAC CCCTCATCCC TGTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001179983.1
    CDS312..3299
    Translation

    Target ORF information:

    RefSeq Version NM_001193054.1
    Organism Bos taurus(cattle)
    Definition Bos taurus echinoderm microtubule associated protein like 4 (EML4), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001193054.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGAAGACA GCCTGCCTAG TCTCGCTTCA GTATGTGCTT CCTCTACACT TTCAAACTTC 
    TGTACTGATG ATAGTATTTC CGCTGCAAGT ACTTCTGATG TTCAGGATCG CCTCTCAGCC
    CTTGAGTTAC GAGTTCAACA GCAAGAAGAT GAAATCACAG TGCTAAAGGC AGCTTTGGCT
    GATGTTTTGA GGCGTCTTGC CATCTCTGAA GATCATGTAG CCTCGGTGAA AAAATCAGTC
    CCAAGTAAAG GCCAGCCAGG CCTTCGAGAA GCTATTTCTA TGTCCTGTAT AACCAACGGG
    AGTGGTACAA ACAGAAAACC AAGTCACACC AGCTCTGTCT CAATCGCAAG AAAAGAAACT
    CTTTCATCTG CTGCTAAAAG TGGTACAGAA AAAAAGAAAG AAAAACCACA AGGACAGAGA
    GAGAAAAAAG AGGACTCTCA CTCTAATGAT CAAAGTCCAC AAATTCGAGC ATCACCTTCT
    CCCCAGCCCT CTTCACAGCC TCTCCAAATA CACAGGCAAA CTCAAGAAAG CAAGAATTCT
    ACTCCCACCA AAAGCATAAA ACGACCATCA ACAGCTGAAA AATCACATAA TTCATGGGAA
    AATTCAGACG ATAGCCGTAA TAAATTATTG AAGGCAGTTT CAACATCAAA ATTAATATCA
    AAAGTTATAA AAAATACAGA CAAGCATAAA GATGTCATCA TCAACCAAGA AGGAGAATAC
    ATTAAAATGT TTATGCGAGG TCGACCAATT ACAATGTTCA TTCCTTCTGA TGTTGAGAAC
    TATGATGACA TCAGAACAGA ACTGCCTCCT GAGAAGCTCA AACTGGAGTG GGTATACGGT
    TATCGAGGAA AGGATTGTCG AGCTAATGTT TACCTTCTTC CTACCGGGGA AATTGTTTAT
    TTCATAGCAT CCGTAGTAGT ACTGTTTAAT TATGAAGAGA GAACTCAACG GCACTACCTG
    GGTCATACAG ACTGTGTAAA ATGCCTTGCT ATACATCCTG ACAAAATTAG GATTGCAACT
    GGACAGATAG CTGGAGTGGA TAAGGATGGA AGGCCCCTGC AACCCCATGT CAGAGTGTGG
    GATTCAGTTA GTCTTTCTAC ACTGCAAATT ATTGGACTTG GAACTTTTGA ACGTGGAGTA
    GGATGCCTGG ATTTTTCAAA AGCAGATTCA GGTGTTCATT TATGTGTTAT CGATGACTCC
    AATGAGCATA TGCTTACTGT GTGGGATTGG CAGAAAAAAT CAAAAGGAGC AGAAATAAAG
    ACAACAAATG AAGTTGTTTT GGCTGTGGGG TTCCATCCAA CAGATGCAAA TATCATAATA
    ACATGTGGTA AATCACATAT TTTTTTCTGG ACCTGGAGTG GCAATTCACT AACAAAAAAA
    CAAGGAGTTT TTGGGAAATA CGAAAAACCA AAATTTGTGC AGTGTTTAGC TTTCTTGGGA
    AATGGAGATG TTCTTGCTGG AGACTCAGGT GGAGTCATAC TCATATGGAG CAAAACTACT
    GTAGAGCCCA CACCTGGGAA AGGGCCTAAA GGTGTTTATC AGATCAGCAA ACAAATCAAA
    GCTCATGATG GCAGTGTGTT CACACTTTGT CAGATGAGAA ATGGGATGTT ATTAACTGGA
    GGTGGGAAAG ACAGAAAAAT AATTCTGTGG GATCATGATC TGAATCCTGA AAGAGAAATA
    GAGGTTCCTG ATCAGTATGG AACAATCAGA GCTGTAGCAG AAGGAAAGGC AGATCAATTT
    TTGGTCGGCA CATCACGAAA CTTTATTTTA CGAGGAACAT TTAATGATGG CTTCCAAATA
    GAAGTACAGG GTCATACAGA TGAGCTTTGG GGTCTTGCCA CACATCCCTT CAAAGACTTG
    CTCTTGACTT GTGCTCAGGA CAGACAGGTG TGCATGTGGA ACTCAGTAGA ACACAGACTG
    GAATGGACCA GGCTTCTAGA TGAACCAGGA CACTGTGCAG ATTTTCATCC AAGTGGCACA
    GTGGTGGCCA TAGGAACGCA CTCAGGCAGG TGGTTTGTTC TGGATGCAGA AACCAGAGAT
    CTAGTTTCTA TCCACACAGA CGGAAATGAG CAGCTTTCGG TGATGCGCTA CTCAATAGAT
    GGCACCCTCC TGGCTGTAGG ATCTCATGAC AACTTCATTT ACCTCTATGC CGTCTCAGAA
    AATGGAAGAA AGTATAGCAG ACATGGAAAG TGCACTGGAC ATTCCAGCTA TATCACACAC
    CTTGACTGGT CCCCAGACAA CAAGTATATA ATGTCTAACT CGGGAGACTA TGAGATCCTG
    TACTGGGACA TTGAACATGG CTGCAAACTA ATGAGGAATC GATCAGAATG TAAGGACATC
    AATTGGACGA CATACACCTG TGTGCTAGGC TTCCAAGTCT TTGGTGTCTG GCCAGAAGGA
    TCTGATGGGA CAGATATCAA CGCGCTGGTA CGATCCCACA ACAGAAAGGT GATCGCTGTT
    GCTGATGACT TTTGCAAAGT CCATCTGTTT CAGTATCCCT GCTCCAAAGC AAAGGCTCCC
    AGTCACAAGT ACAGTGCCCA CAGCAGCCAT GTCACCAATG TCAGTTTTAC TCATAATGAC
    AGTCACCTGA TTTCAACTGG TGGAAAAGAC ATGAGCATCA TTCAGTGGAA ACTTGTGGAA
    AAGTTACCTT TGCCTCAAAA TGAGACTCTA GCGGATCCTA CACTCACCAA AGCCCCTCCC
    TCTTCCATCG AGAGTGTGGT GCAGTCTGAT ACCCCCACTC CGCCTCCTTC CCAGCCCTTA
    AATGAGACAG CTGAAGAAGA AAGTCGGATA AGCAGTTCTC CCACACTTCT GGAGAACAGC
    TTGGAACAGA CCGTGGAGCC CAGCGAAGAC CACAGTGAGG ATCAGAGTGA GGGGGGCAGC
    GAAGACCTCG GTGAGCCTGT TTATGAAGAG CTAGCCAATG AGCTAAGCGA GGAGCAGAGC
    AAAGCCGCCC TTCCCGGGGA CCAGCAAGAC CCCTCATCCC TGTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa238865
    Clone ID Related Accession (Same CDS sequence) NM_001193054.2
    Accession Version NM_001193054.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2988bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-01-16
    Organism Bos taurus(cattle)
    Product echinoderm microtubule-associated protein-like 4
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000011.1. On May 25, 2018 this sequence version replaced NM_001193054.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN03145433, SAMN03145442 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGAAGACA GCCTGCCTAG TCTCGCTTCA GTATGTGCTT CCTCTACACT CTCAAACTTC 
    TGTACTGATG ATAGTATTTC CGCTGCAAGT ACTTCTGATG TTCAGGATCG CCTCTCAGCC
    CTTGAGTTAC GAGTTCAACA GCAAGAAGAT GAAATCACAG TGCTAAAGGC AGCTTTGGCT
    GATGTTTTGA GGCGTCTTGC CATCTCTGAA GATCATGTAG CCTCGGTGAA AAAATCAGTC
    CCAAGTAAAG GCCAGCCAGG CCTTCGAGAA GCTATTTCTA TGTCCTGTAT AACCAACGGG
    AGTGGTACAA ACAGAAAACC AAGTCACACC AGCTCTGTCT CAATCGCAAG AAAAGAAACT
    CTTTCATCTG CTGCTAAAAG TGGTACAGAA AAAAAGAAAG AAAAACCACA AGGACAGAGA
    GAGAAAAAAG AGGACTCTCA CTCTAATGAT CAAAGTCCAC AAATTCGAGC ATCACCTTCT
    CCCCAGCCCT CTTCACAGCC TCTCCAAATA CACAGGCAAA CTCAAGAAAG CAAGAATTCT
    ACTCCCACCA AAAGCATAAA ACGACCATCA ACAGCTGAAA AATCACATAA TTCATGGGAA
    AATTCAGACG ATAGCCGTAA TAAATTATTG AAGGCAGTTT CAACATCAAA ATTAATATCA
    AAAGTTATAA AAAATACAGA CAAGCATAAA GATGTCATCA TCAACCAAGA AGGAGAATAC
    ATTAAAATGT TTATGCGAGG TCGACCAATT ACAATGTTCA TTCCTTCTGA TGTTGAGAAC
    TATGATGACA TCAGAACAGA ACTGCCTCCT GAGAAGCTCA AACTGGAGTG GGTATACGGT
    TATCGAGGAA AGGATTGTCG AGCTAATGTT TACCTTCTTC CTACCGGGGA AATTGTTTAT
    TTCATAGCAT CCGTAGTAGT ACTGTTTAAT TATGAAGAGA GAACTCAACG GCACTACCTG
    GGTCATACAG ACTGTGTAAA ATGCCTTGCT ATACATCCTG ACAAAATTAG GATTGCAACT
    GGACAGATAG CTGGAGTGGA TAAGGATGGA AGGCCCCTGC AACCCCATGT CAGAGTGTGG
    GATTCAGTTA GTCTTTCTAC ACTGCAAATT ATTGGACTTG GAACTTTTGA ACGTGGAGTA
    GGATGCCTGG ATTTTTCAAA AGCAGATTCA GGTGTTCATT TATGTGTTAT CGATGACTCC
    AATGAGCATA TGCTTACTGT GTGGGATTGG CAGAAAAAAT CAAAAGGAGC AGAAATAAAG
    ACAACAAATG AAGTTGTTTT GGCTGTGGGG TTCCATCCAA CAGATGCAAA TATCATAATA
    ACATGTGGTA AATCACATAT TTTTTTCTGG ACCTGGAGTG GCAATTCACT AACAAAAAAA
    CAAGGAGTTT TTGGGAAATA CGAAAAACCA AAATTTGTGC AGTGTTTAGC TTTCTTGGGA
    AATGGAGATG TTCTTGCTGG AGACTCAGGT GGAGTCATAC TCATATGGAG CAAAACTACT
    GTAGAGCCCA CACCTGGGAA AGGGCCTAAA GGTGTTTATC AGATCAGCAA ACAAATCAAA
    GCTCATGATG GCAGTGTGTT CACACTTTGT CAGATGAGAA ATGGGATGTT ATTAACTGGA
    GGTGGGAAAG ACAGAAAAAT AATTCTGTGG GATCATGATC TGAATCCTGA AAGAGAAATA
    GAGGTTCCTG ATCAGTATGG AACAATCAGA GCTGTAGCAG AAGGAAAGGC AGATCAATTT
    TTGGTCGGCA CATCACGAAA CTTTATTTTA CGAGGAACAT TTAATGATGG CTTCCAAATA
    GAAGTACAGG GTCATACAGA TGAGCTTTGG GGTCTTGCCA CACATCCCTT CAAAGACTTG
    CTCTTGACTT GTGCTCAGGA CAGACAGGTG TGCATGTGGA ACTCAGTAGA ACACAGACTG
    GAATGGACCA GGCTTCTAGA TGAACCAGGA CACTGTGCAG ATTTTCATCC AAGTGGCACA
    GTGGTGGCCA TAGGAACGCA CTCAGGCAGG TGGTTTGTTC TGGATGCAGA AACCAGAGAT
    CTAGTTTCTA TCCACACAGA CGGAAATGAG CAGCTTTCGG TGATGCGCTA CTCAATAGAT
    GGCACCCTCC TGGCTGTAGG ATCTCATGAC AACTTCATTT ACCTCTATGC CGTCTCAGAA
    AATGGAAGAA AGTATAGCAG ACATGGAAAG TGCACTGGAC ATTCCAGCTA TATCACACAC
    CTTGACTGGT CCCCAGACAA CAAGTATATA ATGTCTAACT CGGGAGACTA TGAGATCCTG
    TACTGGGACA TTGAACATGG CTGCAAACTA ATGAGGAATC GATCAGAATG TAAGGACATC
    AATTGGACGA CATACACCTG TGTGCTAGGC TTCCAAGTCT TTGGTGTCTG GCCAGAAGGA
    TCTGATGGGA CAGATATCAA CGCGCTGGTA CGATCCCACA ACAGAAAGGT GATCGCTGTT
    GCTGATGACT TTTGCAAAGT CCATCTGTTT CAGTATCCCT GCTCCAAAGC AAAGGCTCCC
    AGTCACAAGT ACAGTGCCCA CAGCAGCCAT GTCACCAATG TCAGTTTTAC TCATAATGAC
    AGTCACCTGA TTTCAACTGG TGGAAAAGAC ATGAGCATCA TTCAGTGGAA ACTTGTGGAA
    AAGTTACCTT TGCCTCAAAA TGAGACTCTA GCGGATCCTA CACTCACCAA AGCCCCTCCC
    TCTTCCATCG AGAGTGTGGT GCAGTCTGAT ACCCCCACTC CGCCTCCTTC CCAGCCCTTA
    AATGAGACAG CTGAAGAAGA AAGTCGGATA AGCAGTTCTC CCACACTTCT GGAGAACAGC
    TTGGAACAGA CCGTGGAGCC CAGCGAAGAC CACAGTGAGG ATCAGAGTGA GGGGGGCAGC
    GAAGACCTCG GTGAGCCTGT TTATGAAGAG CTAGCCAATG AGCTAAGCGA GGAGCAGAGC
    AAAGCCGCCC TTCCCGGGGA CCAGCAAGAC CCCTCATCCC TGTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001179983.1
    CDS312..3299
    Translation

    Target ORF information:

    RefSeq Version NM_001193054.2
    Organism Bos taurus(cattle)
    Definition Bos taurus EMAP like 4 (EML4), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001193054.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGAAGACA GCCTGCCTAG TCTCGCTTCA GTATGTGCTT CCTCTACACT CTCAAACTTC 
    TGTACTGATG ATAGTATTTC CGCTGCAAGT ACTTCTGATG TTCAGGATCG CCTCTCAGCC
    CTTGAGTTAC GAGTTCAACA GCAAGAAGAT GAAATCACAG TGCTAAAGGC AGCTTTGGCT
    GATGTTTTGA GGCGTCTTGC CATCTCTGAA GATCATGTAG CCTCGGTGAA AAAATCAGTC
    CCAAGTAAAG GCCAGCCAGG CCTTCGAGAA GCTATTTCTA TGTCCTGTAT AACCAACGGG
    AGTGGTACAA ACAGAAAACC AAGTCACACC AGCTCTGTCT CAATCGCAAG AAAAGAAACT
    CTTTCATCTG CTGCTAAAAG TGGTACAGAA AAAAAGAAAG AAAAACCACA AGGACAGAGA
    GAGAAAAAAG AGGACTCTCA CTCTAATGAT CAAAGTCCAC AAATTCGAGC ATCACCTTCT
    CCCCAGCCCT CTTCACAGCC TCTCCAAATA CACAGGCAAA CTCAAGAAAG CAAGAATTCT
    ACTCCCACCA AAAGCATAAA ACGACCATCA ACAGCTGAAA AATCACATAA TTCATGGGAA
    AATTCAGACG ATAGCCGTAA TAAATTATTG AAGGCAGTTT CAACATCAAA ATTAATATCA
    AAAGTTATAA AAAATACAGA CAAGCATAAA GATGTCATCA TCAACCAAGA AGGAGAATAC
    ATTAAAATGT TTATGCGAGG TCGACCAATT ACAATGTTCA TTCCTTCTGA TGTTGAGAAC
    TATGATGACA TCAGAACAGA ACTGCCTCCT GAGAAGCTCA AACTGGAGTG GGTATACGGT
    TATCGAGGAA AGGATTGTCG AGCTAATGTT TACCTTCTTC CTACCGGGGA AATTGTTTAT
    TTCATAGCAT CCGTAGTAGT ACTGTTTAAT TATGAAGAGA GAACTCAACG GCACTACCTG
    GGTCATACAG ACTGTGTAAA ATGCCTTGCT ATACATCCTG ACAAAATTAG GATTGCAACT
    GGACAGATAG CTGGAGTGGA TAAGGATGGA AGGCCCCTGC AACCCCATGT CAGAGTGTGG
    GATTCAGTTA GTCTTTCTAC ACTGCAAATT ATTGGACTTG GAACTTTTGA ACGTGGAGTA
    GGATGCCTGG ATTTTTCAAA AGCAGATTCA GGTGTTCATT TATGTGTTAT CGATGACTCC
    AATGAGCATA TGCTTACTGT GTGGGATTGG CAGAAAAAAT CAAAAGGAGC AGAAATAAAG
    ACAACAAATG AAGTTGTTTT GGCTGTGGGG TTCCATCCAA CAGATGCAAA TATCATAATA
    ACATGTGGTA AATCACATAT TTTTTTCTGG ACCTGGAGTG GCAATTCACT AACAAAAAAA
    CAAGGAGTTT TTGGGAAATA CGAAAAACCA AAATTTGTGC AGTGTTTAGC TTTCTTGGGA
    AATGGAGATG TTCTTGCTGG AGACTCAGGT GGAGTCATAC TCATATGGAG CAAAACTACT
    GTAGAGCCCA CACCTGGGAA AGGGCCTAAA GGTGTTTATC AGATCAGCAA ACAAATCAAA
    GCTCATGATG GCAGTGTGTT CACACTTTGT CAGATGAGAA ATGGGATGTT ATTAACTGGA
    GGTGGGAAAG ACAGAAAAAT AATTCTGTGG GATCATGATC TGAATCCTGA AAGAGAAATA
    GAGGTTCCTG ATCAGTATGG AACAATCAGA GCTGTAGCAG AAGGAAAGGC AGATCAATTT
    TTGGTCGGCA CATCACGAAA CTTTATTTTA CGAGGAACAT TTAATGATGG CTTCCAAATA
    GAAGTACAGG GTCATACAGA TGAGCTTTGG GGTCTTGCCA CACATCCCTT CAAAGACTTG
    CTCTTGACTT GTGCTCAGGA CAGACAGGTG TGCATGTGGA ACTCAGTAGA ACACAGACTG
    GAATGGACCA GGCTTCTAGA TGAACCAGGA CACTGTGCAG ATTTTCATCC AAGTGGCACA
    GTGGTGGCCA TAGGAACGCA CTCAGGCAGG TGGTTTGTTC TGGATGCAGA AACCAGAGAT
    CTAGTTTCTA TCCACACAGA CGGAAATGAG CAGCTTTCGG TGATGCGCTA CTCAATAGAT
    GGCACCCTCC TGGCTGTAGG ATCTCATGAC AACTTCATTT ACCTCTATGC CGTCTCAGAA
    AATGGAAGAA AGTATAGCAG ACATGGAAAG TGCACTGGAC ATTCCAGCTA TATCACACAC
    CTTGACTGGT CCCCAGACAA CAAGTATATA ATGTCTAACT CGGGAGACTA TGAGATCCTG
    TACTGGGACA TTGAACATGG CTGCAAACTA ATGAGGAATC GATCAGAATG TAAGGACATC
    AATTGGACGA CATACACCTG TGTGCTAGGC TTCCAAGTCT TTGGTGTCTG GCCAGAAGGA
    TCTGATGGGA CAGATATCAA CGCGCTGGTA CGATCCCACA ACAGAAAGGT GATCGCTGTT
    GCTGATGACT TTTGCAAAGT CCATCTGTTT CAGTATCCCT GCTCCAAAGC AAAGGCTCCC
    AGTCACAAGT ACAGTGCCCA CAGCAGCCAT GTCACCAATG TCAGTTTTAC TCATAATGAC
    AGTCACCTGA TTTCAACTGG TGGAAAAGAC ATGAGCATCA TTCAGTGGAA ACTTGTGGAA
    AAGTTACCTT TGCCTCAAAA TGAGACTCTA GCGGATCCTA CACTCACCAA AGCCCCTCCC
    TCTTCCATCG AGAGTGTGGT GCAGTCTGAT ACCCCCACTC CGCCTCCTTC CCAGCCCTTA
    AATGAGACAG CTGAAGAAGA AAGTCGGATA AGCAGTTCTC CCACACTTCT GGAGAACAGC
    TTGGAACAGA CCGTGGAGCC CAGCGAAGAC CACAGTGAGG ATCAGAGTGA GGGGGGCAGC
    GAAGACCTCG GTGAGCCTGT TTATGAAGAG CTAGCCAATG AGCTAAGCGA GGAGCAGAGC
    AAAGCCGCCC TTCCCGGGGA CCAGCAAGAC CCCTCATCCC TGTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa220579
    Clone ID Related Accession (Same CDS sequence) XM_024999053.1 , XM_024999054.1
    Accession Version XM_024999053.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2970bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product echinoderm microtubule-associated protein-like 4 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGAGGGCAT TTCTTGATGA TAGTATTTCC GCTGCAAGTA CTTCTGATGT TCAGGATCGC 
    CTCTCAGCCC TTGAGTTACG AGTTCAACAG CAAGAAGATG AAATCACAGT GCTAAAGGCA
    GCTTTGGCTG ATGTTTTGAG GCGTCTTGCC ATCTCTGAAG ATCATGTAGC CTCGGTGAAA
    AAATCAGTCC CAAGTAAAGG CCAGCCAGGC CTTCGAGAAG CTATTTCTAT GTCCTGTATA
    ACCAACGGGA GTGGTACAAA CAGAAAACCA AGTCACACCA GCTCTGTCTC AATCGCAAGA
    AAAGAAACTC TTTCATCTGC TGCTAAAAGT GGTACAGAAA AAAAGAAAGA AAAACCACAA
    GGACAGAGAG AGAAAAAAGA GGACTCTCAC TCTAATGATC AAAGTCCACA AATTCGAGCA
    TCACCTTCTC CCCAGCCCTC TTCACAGCCT CTCCAAATAC ACAGGCAAAC TCAAGAAAGC
    AAGAATTCTA CTCCCACCAA AAGCATAAAA CGACCATCAA CAGCTGAAAA ATCACATAAT
    TCATGGGAAA ATTCAGACGA TAGCCGTAAT AAATTATTGA AGGCAGTTTC AACATCAAAA
    TTAATATCAA AAGTTATAAA AAATACAGAC AAGCATAAAG ATGTCATCAT CAACCAAGCA
    AAAATGTCAA CCCGTGAAAA AAACAGCCAA GAAGGAGAAT ACATTAAAAT GTTTATGCGA
    GGTCGACCAA TTACAATGTT CATTCCTTCT GATGTTGAGA ACTATGATGA CATCAGAACA
    GAACTGCCTC CTGAGAAGCT CAAACTGGAG TGGGTATACG GTTATCGAGG AAAGGATTGT
    CGAGCTAATG TTTACCTTCT TCCTACCGGG GAAATTGTTT ATTTCATAGC ATCCGTAGTA
    GTACTGTTTA ATTATGAAGA GAGAACTCAA CGGCACTACC TGGGTCATAC AGACTGTGTA
    AAATGCCTTG CTATACATCC TGACAAAATT AGGATTGCAA CTGGACAGAT AGCTGGAGTG
    GATAAGGATG GAAGGCCCCT GCAACCCCAT GTCAGAGTGT GGGATTCAGT TAGTCTTTCT
    ACACTGCAAA TTATTGGACT TGGAACTTTT GAACGTGGAG TAGGATGCCT GGATTTTTCA
    AAAGCAGATT CAGGTGTTCA TTTATGTGTT ATCGATGACT CCAATGAGCA TATGCTTACT
    GTGTGGGATT GGCAGAAAAA ATCAAAAGGA GCAGAAATAA AGACAACAAA TGAAGTTGTT
    TTGGCTGTGG GGTTCCATCC AACAGATGCA AATATCATAA TAACATGTGG TAAATCACAT
    ATTTTTTTCT GGACCTGGAG TGGCAATTCA CTAACAAAAA AACAAGGAGT TTTTGGGAAA
    TACGAAAAAC CAAAATTTGT GCAGTGTTTA GCTTTCTTGG GAAATGGAGA TGTTCTTGCT
    GGAGACTCAG GTGGAGTCAT ACTCATATGG AGCAAAACTA CTGTAGAGCC CACACCTGGG
    AAAGGGCCTA AAGGTGTTTA TCAGATCAGC AAACAAATCA AAGCTCATGA TGGCAGTGTG
    TTCACACTTT GTCAGATGAG AAATGGGATG TTATTAACTG GAGGTGGGAA AGACAGAAAA
    ATAATTCTGT GGGATCATGA TCTGAATCCT GAAAGAGAAA TAGAGGTTCC TGATCAGTAT
    GGAACAATCA GAGCTGTAGC AGAAGGAAAG GCAGATCAAT TTTTGGTCGG CACATCACGA
    AACTTTATTT TACGAGGAAC ATTTAATGAT GGCTTCCAAA TAGAAGTACA GGGTCATACA
    GATGAGCTTT GGGGTCTTGC CACACATCCC TTCAAAGACT TGCTCTTGAC TTGTGCTCAG
    GACAGACAGG TGTGCATGTG GAACTCAGTA GAACACAGAC TGGAATGGAC CAGGCTTCTA
    GATGAACCAG GACACTGTGC AGATTTTCAT CCAAGTGGCA CAGTGGTGGC CATAGGAACG
    CACTCAGGCA GGTGGTTTGT TCTGGATGCA GAAACCAGAG ATCTAGTTTC TATCCACACA
    GACGGAAATG AGCAGCTTTC GGTGATGCGC TACTCAATAG ATGGCACCCT CCTGGCTGTA
    GGATCTCATG ACAACTTCAT TTACCTCTAT GCCGTCTCAG AAAATGGAAG AAAGTATAGC
    AGACATGGAA AGTGCACTGG ACATTCCAGC TATATCACAC ACCTTGACTG GTCCCCAGAC
    AACAAGTATA TAATGTCTAA CTCGGGAGAC TATGAGATCC TGTACTGGGA CATTGAACAT
    GGCTGCAAAC TAATGAGGAA TCGATCAGAA TGTAAGGACA TCAATTGGAC GACATACACC
    TGTGTGCTAG GCTTCCAAGT CTTTGGTGTC TGGCCAGAAG GATCTGATGG GACAGATATC
    AACGCGCTGG TACGATCCCA CAACAGAAAG GTGATCGCTG TTGCTGATGA CTTTTGCAAA
    GTCCATCTGT TTCAGTATCC CTGCTCCAAA GCAAAGGCTC CCAGTCACAA GTACAGTGCC
    CACAGCAGCC ATGTCACCAA TGTCAGTTTT ACTCATAATG ACAGTCACCT GATTTCAACT
    GGTGGAAAAG ACATGAGCAT CATTCAGTGG AAACTTGTGG AAAAGTTACC TTTGCCTCAA
    AATGAGACTC TAGCGGATCC TACACTCACC AAAGCCCCTC CCTCTTCCAT CGAGAGTGTG
    GTGCAGTCTG ATACCCCCAC TCCGCCTCCT TCCCAGCCCT TAAATGAGAC AGCTGAAGAA
    GAAAGTCGGA TAAGCAGTTC TCCCACACTT CTGGAGAACA GCTTGGAACA GACCGTGGAG
    CCCAGCGAAG ACCACAGTGA GGATCAGAGT GAGGGGGGCA GCGAAGACCT CGGTGAGCCT
    GTTTATGAAG AGCTAGCCAA TGAGCTAAGC GAGGAGCAGA GCAAAGCCGC CCTTCCCGGG
    GACCAGCAAG ACCCCTCATC CCTGTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024854821.1
    CDS588..3557
    Translation

    Target ORF information:

    RefSeq Version XM_024999053.1
    Organism Bos taurus(cattle)
    Definition Bos taurus echinoderm microtubule associated protein like 4 (EML4), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024999053.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGAGGGCAT TTCTTGATGA TAGTATTTCC GCTGCAAGTA CTTCTGATGT TCAGGATCGC 
    CTCTCAGCCC TTGAGTTACG AGTTCAACAG CAAGAAGATG AAATCACAGT GCTAAAGGCA
    GCTTTGGCTG ATGTTTTGAG GCGTCTTGCC ATCTCTGAAG ATCATGTAGC CTCGGTGAAA
    AAATCAGTCC CAAGTAAAGG CCAGCCAGGC CTTCGAGAAG CTATTTCTAT GTCCTGTATA
    ACCAACGGGA GTGGTACAAA CAGAAAACCA AGTCACACCA GCTCTGTCTC AATCGCAAGA
    AAAGAAACTC TTTCATCTGC TGCTAAAAGT GGTACAGAAA AAAAGAAAGA AAAACCACAA
    GGACAGAGAG AGAAAAAAGA GGACTCTCAC TCTAATGATC AAAGTCCACA AATTCGAGCA
    TCACCTTCTC CCCAGCCCTC TTCACAGCCT CTCCAAATAC ACAGGCAAAC TCAAGAAAGC
    AAGAATTCTA CTCCCACCAA AAGCATAAAA CGACCATCAA CAGCTGAAAA ATCACATAAT
    TCATGGGAAA ATTCAGACGA TAGCCGTAAT AAATTATTGA AGGCAGTTTC AACATCAAAA
    TTAATATCAA AAGTTATAAA AAATACAGAC AAGCATAAAG ATGTCATCAT CAACCAAGCA
    AAAATGTCAA CCCGTGAAAA AAACAGCCAA GAAGGAGAAT ACATTAAAAT GTTTATGCGA
    GGTCGACCAA TTACAATGTT CATTCCTTCT GATGTTGAGA ACTATGATGA CATCAGAACA
    GAACTGCCTC CTGAGAAGCT CAAACTGGAG TGGGTATACG GTTATCGAGG AAAGGATTGT
    CGAGCTAATG TTTACCTTCT TCCTACCGGG GAAATTGTTT ATTTCATAGC ATCCGTAGTA
    GTACTGTTTA ATTATGAAGA GAGAACTCAA CGGCACTACC TGGGTCATAC AGACTGTGTA
    AAATGCCTTG CTATACATCC TGACAAAATT AGGATTGCAA CTGGACAGAT AGCTGGAGTG
    GATAAGGATG GAAGGCCCCT GCAACCCCAT GTCAGAGTGT GGGATTCAGT TAGTCTTTCT
    ACACTGCAAA TTATTGGACT TGGAACTTTT GAACGTGGAG TAGGATGCCT GGATTTTTCA
    AAAGCAGATT CAGGTGTTCA TTTATGTGTT ATCGATGACT CCAATGAGCA TATGCTTACT
    GTGTGGGATT GGCAGAAAAA ATCAAAAGGA GCAGAAATAA AGACAACAAA TGAAGTTGTT
    TTGGCTGTGG GGTTCCATCC AACAGATGCA AATATCATAA TAACATGTGG TAAATCACAT
    ATTTTTTTCT GGACCTGGAG TGGCAATTCA CTAACAAAAA AACAAGGAGT TTTTGGGAAA
    TACGAAAAAC CAAAATTTGT GCAGTGTTTA GCTTTCTTGG GAAATGGAGA TGTTCTTGCT
    GGAGACTCAG GTGGAGTCAT ACTCATATGG AGCAAAACTA CTGTAGAGCC CACACCTGGG
    AAAGGGCCTA AAGGTGTTTA TCAGATCAGC AAACAAATCA AAGCTCATGA TGGCAGTGTG
    TTCACACTTT GTCAGATGAG AAATGGGATG TTATTAACTG GAGGTGGGAA AGACAGAAAA
    ATAATTCTGT GGGATCATGA TCTGAATCCT GAAAGAGAAA TAGAGGTTCC TGATCAGTAT
    GGAACAATCA GAGCTGTAGC AGAAGGAAAG GCAGATCAAT TTTTGGTCGG CACATCACGA
    AACTTTATTT TACGAGGAAC ATTTAATGAT GGCTTCCAAA TAGAAGTACA GGGTCATACA
    GATGAGCTTT GGGGTCTTGC CACACATCCC TTCAAAGACT TGCTCTTGAC TTGTGCTCAG
    GACAGACAGG TGTGCATGTG GAACTCAGTA GAACACAGAC TGGAATGGAC CAGGCTTCTA
    GATGAACCAG GACACTGTGC AGATTTTCAT CCAAGTGGCA CAGTGGTGGC CATAGGAACG
    CACTCAGGCA GGTGGTTTGT TCTGGATGCA GAAACCAGAG ATCTAGTTTC TATCCACACA
    GACGGAAATG AGCAGCTTTC GGTGATGCGC TACTCAATAG ATGGCACCCT CCTGGCTGTA
    GGATCTCATG ACAACTTCAT TTACCTCTAT GCCGTCTCAG AAAATGGAAG AAAGTATAGC
    AGACATGGAA AGTGCACTGG ACATTCCAGC TATATCACAC ACCTTGACTG GTCCCCAGAC
    AACAAGTATA TAATGTCTAA CTCGGGAGAC TATGAGATCC TGTACTGGGA CATTGAACAT
    GGCTGCAAAC TAATGAGGAA TCGATCAGAA TGTAAGGACA TCAATTGGAC GACATACACC
    TGTGTGCTAG GCTTCCAAGT CTTTGGTGTC TGGCCAGAAG GATCTGATGG GACAGATATC
    AACGCGCTGG TACGATCCCA CAACAGAAAG GTGATCGCTG TTGCTGATGA CTTTTGCAAA
    GTCCATCTGT TTCAGTATCC CTGCTCCAAA GCAAAGGCTC CCAGTCACAA GTACAGTGCC
    CACAGCAGCC ATGTCACCAA TGTCAGTTTT ACTCATAATG ACAGTCACCT GATTTCAACT
    GGTGGAAAAG ACATGAGCAT CATTCAGTGG AAACTTGTGG AAAAGTTACC TTTGCCTCAA
    AATGAGACTC TAGCGGATCC TACACTCACC AAAGCCCCTC CCTCTTCCAT CGAGAGTGTG
    GTGCAGTCTG ATACCCCCAC TCCGCCTCCT TCCCAGCCCT TAAATGAGAC AGCTGAAGAA
    GAAAGTCGGA TAAGCAGTTC TCCCACACTT CTGGAGAACA GCTTGGAACA GACCGTGGAG
    CCCAGCGAAG ACCACAGTGA GGATCAGAGT GAGGGGGGCA GCGAAGACCT CGGTGAGCCT
    GTTTATGAAG AGCTAGCCAA TGAGCTAAGC GAGGAGCAGA GCAAAGCCGC CCTTCCCGGG
    GACCAGCAAG ACCCCTCATC CCTGTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa220579
    Clone ID Related Accession (Same CDS sequence) XM_024999053.1 , XM_024999054.1
    Accession Version XM_024999054.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2970bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product echinoderm microtubule-associated protein-like 4 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGAGGGCAT TTCTTGATGA TAGTATTTCC GCTGCAAGTA CTTCTGATGT TCAGGATCGC 
    CTCTCAGCCC TTGAGTTACG AGTTCAACAG CAAGAAGATG AAATCACAGT GCTAAAGGCA
    GCTTTGGCTG ATGTTTTGAG GCGTCTTGCC ATCTCTGAAG ATCATGTAGC CTCGGTGAAA
    AAATCAGTCC CAAGTAAAGG CCAGCCAGGC CTTCGAGAAG CTATTTCTAT GTCCTGTATA
    ACCAACGGGA GTGGTACAAA CAGAAAACCA AGTCACACCA GCTCTGTCTC AATCGCAAGA
    AAAGAAACTC TTTCATCTGC TGCTAAAAGT GGTACAGAAA AAAAGAAAGA AAAACCACAA
    GGACAGAGAG AGAAAAAAGA GGACTCTCAC TCTAATGATC AAAGTCCACA AATTCGAGCA
    TCACCTTCTC CCCAGCCCTC TTCACAGCCT CTCCAAATAC ACAGGCAAAC TCAAGAAAGC
    AAGAATTCTA CTCCCACCAA AAGCATAAAA CGACCATCAA CAGCTGAAAA ATCACATAAT
    TCATGGGAAA ATTCAGACGA TAGCCGTAAT AAATTATTGA AGGCAGTTTC AACATCAAAA
    TTAATATCAA AAGTTATAAA AAATACAGAC AAGCATAAAG ATGTCATCAT CAACCAAGCA
    AAAATGTCAA CCCGTGAAAA AAACAGCCAA GAAGGAGAAT ACATTAAAAT GTTTATGCGA
    GGTCGACCAA TTACAATGTT CATTCCTTCT GATGTTGAGA ACTATGATGA CATCAGAACA
    GAACTGCCTC CTGAGAAGCT CAAACTGGAG TGGGTATACG GTTATCGAGG AAAGGATTGT
    CGAGCTAATG TTTACCTTCT TCCTACCGGG GAAATTGTTT ATTTCATAGC ATCCGTAGTA
    GTACTGTTTA ATTATGAAGA GAGAACTCAA CGGCACTACC TGGGTCATAC AGACTGTGTA
    AAATGCCTTG CTATACATCC TGACAAAATT AGGATTGCAA CTGGACAGAT AGCTGGAGTG
    GATAAGGATG GAAGGCCCCT GCAACCCCAT GTCAGAGTGT GGGATTCAGT TAGTCTTTCT
    ACACTGCAAA TTATTGGACT TGGAACTTTT GAACGTGGAG TAGGATGCCT GGATTTTTCA
    AAAGCAGATT CAGGTGTTCA TTTATGTGTT ATCGATGACT CCAATGAGCA TATGCTTACT
    GTGTGGGATT GGCAGAAAAA ATCAAAAGGA GCAGAAATAA AGACAACAAA TGAAGTTGTT
    TTGGCTGTGG GGTTCCATCC AACAGATGCA AATATCATAA TAACATGTGG TAAATCACAT
    ATTTTTTTCT GGACCTGGAG TGGCAATTCA CTAACAAAAA AACAAGGAGT TTTTGGGAAA
    TACGAAAAAC CAAAATTTGT GCAGTGTTTA GCTTTCTTGG GAAATGGAGA TGTTCTTGCT
    GGAGACTCAG GTGGAGTCAT ACTCATATGG AGCAAAACTA CTGTAGAGCC CACACCTGGG
    AAAGGGCCTA AAGGTGTTTA TCAGATCAGC AAACAAATCA AAGCTCATGA TGGCAGTGTG
    TTCACACTTT GTCAGATGAG AAATGGGATG TTATTAACTG GAGGTGGGAA AGACAGAAAA
    ATAATTCTGT GGGATCATGA TCTGAATCCT GAAAGAGAAA TAGAGGTTCC TGATCAGTAT
    GGAACAATCA GAGCTGTAGC AGAAGGAAAG GCAGATCAAT TTTTGGTCGG CACATCACGA
    AACTTTATTT TACGAGGAAC ATTTAATGAT GGCTTCCAAA TAGAAGTACA GGGTCATACA
    GATGAGCTTT GGGGTCTTGC CACACATCCC TTCAAAGACT TGCTCTTGAC TTGTGCTCAG
    GACAGACAGG TGTGCATGTG GAACTCAGTA GAACACAGAC TGGAATGGAC CAGGCTTCTA
    GATGAACCAG GACACTGTGC AGATTTTCAT CCAAGTGGCA CAGTGGTGGC CATAGGAACG
    CACTCAGGCA GGTGGTTTGT TCTGGATGCA GAAACCAGAG ATCTAGTTTC TATCCACACA
    GACGGAAATG AGCAGCTTTC GGTGATGCGC TACTCAATAG ATGGCACCCT CCTGGCTGTA
    GGATCTCATG ACAACTTCAT TTACCTCTAT GCCGTCTCAG AAAATGGAAG AAAGTATAGC
    AGACATGGAA AGTGCACTGG ACATTCCAGC TATATCACAC ACCTTGACTG GTCCCCAGAC
    AACAAGTATA TAATGTCTAA CTCGGGAGAC TATGAGATCC TGTACTGGGA CATTGAACAT
    GGCTGCAAAC TAATGAGGAA TCGATCAGAA TGTAAGGACA TCAATTGGAC GACATACACC
    TGTGTGCTAG GCTTCCAAGT CTTTGGTGTC TGGCCAGAAG GATCTGATGG GACAGATATC
    AACGCGCTGG TACGATCCCA CAACAGAAAG GTGATCGCTG TTGCTGATGA CTTTTGCAAA
    GTCCATCTGT TTCAGTATCC CTGCTCCAAA GCAAAGGCTC CCAGTCACAA GTACAGTGCC
    CACAGCAGCC ATGTCACCAA TGTCAGTTTT ACTCATAATG ACAGTCACCT GATTTCAACT
    GGTGGAAAAG ACATGAGCAT CATTCAGTGG AAACTTGTGG AAAAGTTACC TTTGCCTCAA
    AATGAGACTC TAGCGGATCC TACACTCACC AAAGCCCCTC CCTCTTCCAT CGAGAGTGTG
    GTGCAGTCTG ATACCCCCAC TCCGCCTCCT TCCCAGCCCT TAAATGAGAC AGCTGAAGAA
    GAAAGTCGGA TAAGCAGTTC TCCCACACTT CTGGAGAACA GCTTGGAACA GACCGTGGAG
    CCCAGCGAAG ACCACAGTGA GGATCAGAGT GAGGGGGGCA GCGAAGACCT CGGTGAGCCT
    GTTTATGAAG AGCTAGCCAA TGAGCTAAGC GAGGAGCAGA GCAAAGCCGC CCTTCCCGGG
    GACCAGCAAG ACCCCTCATC CCTGTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024854822.1
    CDS659..3628
    Translation

    Target ORF information:

    RefSeq Version XM_024999054.1
    Organism Bos taurus(cattle)
    Definition Bos taurus echinoderm microtubule associated protein like 4 (EML4), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024999054.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGAGGGCAT TTCTTGATGA TAGTATTTCC GCTGCAAGTA CTTCTGATGT TCAGGATCGC 
    CTCTCAGCCC TTGAGTTACG AGTTCAACAG CAAGAAGATG AAATCACAGT GCTAAAGGCA
    GCTTTGGCTG ATGTTTTGAG GCGTCTTGCC ATCTCTGAAG ATCATGTAGC CTCGGTGAAA
    AAATCAGTCC CAAGTAAAGG CCAGCCAGGC CTTCGAGAAG CTATTTCTAT GTCCTGTATA
    ACCAACGGGA GTGGTACAAA CAGAAAACCA AGTCACACCA GCTCTGTCTC AATCGCAAGA
    AAAGAAACTC TTTCATCTGC TGCTAAAAGT GGTACAGAAA AAAAGAAAGA AAAACCACAA
    GGACAGAGAG AGAAAAAAGA GGACTCTCAC TCTAATGATC AAAGTCCACA AATTCGAGCA
    TCACCTTCTC CCCAGCCCTC TTCACAGCCT CTCCAAATAC ACAGGCAAAC TCAAGAAAGC
    AAGAATTCTA CTCCCACCAA AAGCATAAAA CGACCATCAA CAGCTGAAAA ATCACATAAT
    TCATGGGAAA ATTCAGACGA TAGCCGTAAT AAATTATTGA AGGCAGTTTC AACATCAAAA
    TTAATATCAA AAGTTATAAA AAATACAGAC AAGCATAAAG ATGTCATCAT CAACCAAGCA
    AAAATGTCAA CCCGTGAAAA AAACAGCCAA GAAGGAGAAT ACATTAAAAT GTTTATGCGA
    GGTCGACCAA TTACAATGTT CATTCCTTCT GATGTTGAGA ACTATGATGA CATCAGAACA
    GAACTGCCTC CTGAGAAGCT CAAACTGGAG TGGGTATACG GTTATCGAGG AAAGGATTGT
    CGAGCTAATG TTTACCTTCT TCCTACCGGG GAAATTGTTT ATTTCATAGC ATCCGTAGTA
    GTACTGTTTA ATTATGAAGA GAGAACTCAA CGGCACTACC TGGGTCATAC AGACTGTGTA
    AAATGCCTTG CTATACATCC TGACAAAATT AGGATTGCAA CTGGACAGAT AGCTGGAGTG
    GATAAGGATG GAAGGCCCCT GCAACCCCAT GTCAGAGTGT GGGATTCAGT TAGTCTTTCT
    ACACTGCAAA TTATTGGACT TGGAACTTTT GAACGTGGAG TAGGATGCCT GGATTTTTCA
    AAAGCAGATT CAGGTGTTCA TTTATGTGTT ATCGATGACT CCAATGAGCA TATGCTTACT
    GTGTGGGATT GGCAGAAAAA ATCAAAAGGA GCAGAAATAA AGACAACAAA TGAAGTTGTT
    TTGGCTGTGG GGTTCCATCC AACAGATGCA AATATCATAA TAACATGTGG TAAATCACAT
    ATTTTTTTCT GGACCTGGAG TGGCAATTCA CTAACAAAAA AACAAGGAGT TTTTGGGAAA
    TACGAAAAAC CAAAATTTGT GCAGTGTTTA GCTTTCTTGG GAAATGGAGA TGTTCTTGCT
    GGAGACTCAG GTGGAGTCAT ACTCATATGG AGCAAAACTA CTGTAGAGCC CACACCTGGG
    AAAGGGCCTA AAGGTGTTTA TCAGATCAGC AAACAAATCA AAGCTCATGA TGGCAGTGTG
    TTCACACTTT GTCAGATGAG AAATGGGATG TTATTAACTG GAGGTGGGAA AGACAGAAAA
    ATAATTCTGT GGGATCATGA TCTGAATCCT GAAAGAGAAA TAGAGGTTCC TGATCAGTAT
    GGAACAATCA GAGCTGTAGC AGAAGGAAAG GCAGATCAAT TTTTGGTCGG CACATCACGA
    AACTTTATTT TACGAGGAAC ATTTAATGAT GGCTTCCAAA TAGAAGTACA GGGTCATACA
    GATGAGCTTT GGGGTCTTGC CACACATCCC TTCAAAGACT TGCTCTTGAC TTGTGCTCAG
    GACAGACAGG TGTGCATGTG GAACTCAGTA GAACACAGAC TGGAATGGAC CAGGCTTCTA
    GATGAACCAG GACACTGTGC AGATTTTCAT CCAAGTGGCA CAGTGGTGGC CATAGGAACG
    CACTCAGGCA GGTGGTTTGT TCTGGATGCA GAAACCAGAG ATCTAGTTTC TATCCACACA
    GACGGAAATG AGCAGCTTTC GGTGATGCGC TACTCAATAG ATGGCACCCT CCTGGCTGTA
    GGATCTCATG ACAACTTCAT TTACCTCTAT GCCGTCTCAG AAAATGGAAG AAAGTATAGC
    AGACATGGAA AGTGCACTGG ACATTCCAGC TATATCACAC ACCTTGACTG GTCCCCAGAC
    AACAAGTATA TAATGTCTAA CTCGGGAGAC TATGAGATCC TGTACTGGGA CATTGAACAT
    GGCTGCAAAC TAATGAGGAA TCGATCAGAA TGTAAGGACA TCAATTGGAC GACATACACC
    TGTGTGCTAG GCTTCCAAGT CTTTGGTGTC TGGCCAGAAG GATCTGATGG GACAGATATC
    AACGCGCTGG TACGATCCCA CAACAGAAAG GTGATCGCTG TTGCTGATGA CTTTTGCAAA
    GTCCATCTGT TTCAGTATCC CTGCTCCAAA GCAAAGGCTC CCAGTCACAA GTACAGTGCC
    CACAGCAGCC ATGTCACCAA TGTCAGTTTT ACTCATAATG ACAGTCACCT GATTTCAACT
    GGTGGAAAAG ACATGAGCAT CATTCAGTGG AAACTTGTGG AAAAGTTACC TTTGCCTCAA
    AATGAGACTC TAGCGGATCC TACACTCACC AAAGCCCCTC CCTCTTCCAT CGAGAGTGTG
    GTGCAGTCTG ATACCCCCAC TCCGCCTCCT TCCCAGCCCT TAAATGAGAC AGCTGAAGAA
    GAAAGTCGGA TAAGCAGTTC TCCCACACTT CTGGAGAACA GCTTGGAACA GACCGTGGAG
    CCCAGCGAAG ACCACAGTGA GGATCAGAGT GAGGGGGGCA GCGAAGACCT CGGTGAGCCT
    GTTTATGAAG AGCTAGCCAA TGAGCTAAGC GAGGAGCAGA GCAAAGCCGC CCTTCCCGGG
    GACCAGCAAG ACCCCTCATC CCTGTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa220580
    Clone ID Related Accession (Same CDS sequence) XM_024999055.1
    Accession Version XM_024999055.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2946bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product echinoderm microtubule-associated protein-like 4 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGACGGTT TCGCCGGCAG CCTCGATGAT AGTATTTCCG CTGCAAGTAC TTCTGATGTT 
    CAGGATCGCC TCTCAGCCCT TGAGTTACGA GTTCAACAGC AAGAAGATGA AATCACAGTG
    CTAAAGGCAG CTTTGGCTGA TGTTTTGAGG CGTCTTGCCA TCTCTGAAGA TCATGTAGCC
    TCGGTGAAAA AATCAGTCCC AAGTAAAGGC CAGCCAGGCC TTCGAGAAGC TATTTCTATG
    TCCTGTATAA CCAACGGGAG TGGTACAAAC AGAAAACCAA GTCACACCAG CTCTGTCTCA
    ATCGCAAGAA AAGAAACTCT TTCATCTGCT GCTAAAAGTG GTACAGAAAA AAAGAAAGAA
    AAACCACAAG GACAGAGAGA GAAAAAAGAG GACTCTCACT CTAATGATCA AAGTCCACAA
    ATTCGAGCAT CACCTTCTCC CCAGCCCTCT TCACAGCCTC TCCAAATACA CAGGCAAACT
    CAAGAAAGCA AGAATTCTAC TCCCACCAAA AGCATAAAAC GACCATCAAC AGCTGAAAAA
    TCACATAATT CATGGGAAAA TTCAGACGAT AGCCGTAATA AATTATTGAA GGCAGTTTCA
    ACATCAAAAT TAATATCAAA AGTTATAAAA AATACAGACA AGCATAAAGA TGTCATCATC
    AACCAAGAAG GAGAATACAT TAAAATGTTT ATGCGAGGTC GACCAATTAC AATGTTCATT
    CCTTCTGATG TTGAGAACTA TGATGACATC AGAACAGAAC TGCCTCCTGA GAAGCTCAAA
    CTGGAGTGGG TATACGGTTA TCGAGGAAAG GATTGTCGAG CTAATGTTTA CCTTCTTCCT
    ACCGGGGAAA TTGTTTATTT CATAGCATCC GTAGTAGTAC TGTTTAATTA TGAAGAGAGA
    ACTCAACGGC ACTACCTGGG TCATACAGAC TGTGTAAAAT GCCTTGCTAT ACATCCTGAC
    AAAATTAGGA TTGCAACTGG ACAGATAGCT GGAGTGGATA AGGATGGAAG GCCCCTGCAA
    CCCCATGTCA GAGTGTGGGA TTCAGTTAGT CTTTCTACAC TGCAAATTAT TGGACTTGGA
    ACTTTTGAAC GTGGAGTAGG ATGCCTGGAT TTTTCAAAAG CAGATTCAGG TGTTCATTTA
    TGTGTTATCG ATGACTCCAA TGAGCATATG CTTACTGTGT GGGATTGGCA GAAAAAATCA
    AAAGGAGCAG AAATAAAGAC AACAAATGAA GTTGTTTTGG CTGTGGGGTT CCATCCAACA
    GATGCAAATA TCATAATAAC ATGTGGTAAA TCACATATTT TTTTCTGGAC CTGGAGTGGC
    AATTCACTAA CAAAAAAACA AGGAGTTTTT GGGAAATACG AAAAACCAAA ATTTGTGCAG
    TGTTTAGCTT TCTTGGGAAA TGGAGATGTT CTTGCTGGAG ACTCAGGTGG AGTCATACTC
    ATATGGAGCA AAACTACTGT AGAGCCCACA CCTGGGAAAG GGCCTAAAGG TGTTTATCAG
    ATCAGCAAAC AAATCAAAGC TCATGATGGC AGTGTGTTCA CACTTTGTCA GATGAGAAAT
    GGGATGTTAT TAACTGGAGG TGGGAAAGAC AGAAAAATAA TTCTGTGGGA TCATGATCTG
    AATCCTGAAA GAGAAATAGA GGTTCCTGAT CAGTATGGAA CAATCAGAGC TGTAGCAGAA
    GGAAAGGCAG ATCAATTTTT GGTCGGCACA TCACGAAACT TTATTTTACG AGGAACATTT
    AATGATGGCT TCCAAATAGA AGTACAGGGT CATACAGATG AGCTTTGGGG TCTTGCCACA
    CATCCCTTCA AAGACTTGCT CTTGACTTGT GCTCAGGACA GACAGGTGTG CATGTGGAAC
    TCAGTAGAAC ACAGACTGGA ATGGACCAGG CTTCTAGATG AACCAGGACA CTGTGCAGAT
    TTTCATCCAA GTGGCACAGT GGTGGCCATA GGAACGCACT CAGGCAGGTG GTTTGTTCTG
    GATGCAGAAA CCAGAGATCT AGTTTCTATC CACACAGACG GAAATGAGCA GCTTTCGGTG
    ATGCGCTACT CAATAGATGG CACCCTCCTG GCTGTAGGAT CTCATGACAA CTTCATTTAC
    CTCTATGCCG TCTCAGAAAA TGGAAGAAAG TATAGCAGAC ATGGAAAGTG CACTGGACAT
    TCCAGCTATA TCACACACCT TGACTGGTCC CCAGACAACA AGTATATAAT GTCTAACTCG
    GGAGACTATG AGATCCTGTA CTGGGACATT GAACATGGCT GCAAACTAAT GAGGAATCGA
    TCAGAATGTA AGGACATCAA TTGGACGACA TACACCTGTG TGCTAGGCTT CCAAGTCTTT
    GGTGTCTGGC CAGAAGGATC TGATGGGACA GATATCAACG CGCTGGTACG ATCCCACAAC
    AGAAAGGTGA TCGCTGTTGC TGATGACTTT TGCAAAGTCC ATCTGTTTCA GTATCCCTGC
    TCCAAAGCAA AGGCTCCCAG TCACAAGTAC AGTGCCCACA GCAGCCATGT CACCAATGTC
    AGTTTTACTC ATAATGACAG TCACCTGATT TCAACTGGTG GAAAAGACAT GAGCATCATT
    CAGTGGAAAC TTGTGGAAAA GTTACCTTTG CCTCAAAATG AGACTCTAGC GGATCCTACA
    CTCACCAAAG CCCCTCCCTC TTCCATCGAG AGTGTGGTGC AGTCTGATAC CCCCACTCCG
    CCTCCTTCCC AGCCCTTAAA TGAGACAGCT GAAGAAGAAA GTCGGATAAG CAGTTCTCCC
    ACACTTCTGG AGAACAGCTT GGAACAGACC GTGGAGCCCA GCGAAGACCA CAGTGAGGAT
    CAGAGTGAGG GGGGCAGCGA AGACCTCGGT GAGCCTGTTT ATGAAGAGCT AGCCAATGAG
    CTAAGCGAGG AGCAGAGCAA AGCCGCCCTT CCCGGGGACC AGCAAGACCC CTCATCCCTG
    TCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024854823.1
    CDS290..3235
    Translation

    Target ORF information:

    RefSeq Version XM_024999055.1
    Organism Bos taurus(cattle)
    Definition Bos taurus echinoderm microtubule associated protein like 4 (EML4), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024999055.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGACGGTT TCGCCGGCAG CCTCGATGAT AGTATTTCCG CTGCAAGTAC TTCTGATGTT 
    CAGGATCGCC TCTCAGCCCT TGAGTTACGA GTTCAACAGC AAGAAGATGA AATCACAGTG
    CTAAAGGCAG CTTTGGCTGA TGTTTTGAGG CGTCTTGCCA TCTCTGAAGA TCATGTAGCC
    TCGGTGAAAA AATCAGTCCC AAGTAAAGGC CAGCCAGGCC TTCGAGAAGC TATTTCTATG
    TCCTGTATAA CCAACGGGAG TGGTACAAAC AGAAAACCAA GTCACACCAG CTCTGTCTCA
    ATCGCAAGAA AAGAAACTCT TTCATCTGCT GCTAAAAGTG GTACAGAAAA AAAGAAAGAA
    AAACCACAAG GACAGAGAGA GAAAAAAGAG GACTCTCACT CTAATGATCA AAGTCCACAA
    ATTCGAGCAT CACCTTCTCC CCAGCCCTCT TCACAGCCTC TCCAAATACA CAGGCAAACT
    CAAGAAAGCA AGAATTCTAC TCCCACCAAA AGCATAAAAC GACCATCAAC AGCTGAAAAA
    TCACATAATT CATGGGAAAA TTCAGACGAT AGCCGTAATA AATTATTGAA GGCAGTTTCA
    ACATCAAAAT TAATATCAAA AGTTATAAAA AATACAGACA AGCATAAAGA TGTCATCATC
    AACCAAGAAG GAGAATACAT TAAAATGTTT ATGCGAGGTC GACCAATTAC AATGTTCATT
    CCTTCTGATG TTGAGAACTA TGATGACATC AGAACAGAAC TGCCTCCTGA GAAGCTCAAA
    CTGGAGTGGG TATACGGTTA TCGAGGAAAG GATTGTCGAG CTAATGTTTA CCTTCTTCCT
    ACCGGGGAAA TTGTTTATTT CATAGCATCC GTAGTAGTAC TGTTTAATTA TGAAGAGAGA
    ACTCAACGGC ACTACCTGGG TCATACAGAC TGTGTAAAAT GCCTTGCTAT ACATCCTGAC
    AAAATTAGGA TTGCAACTGG ACAGATAGCT GGAGTGGATA AGGATGGAAG GCCCCTGCAA
    CCCCATGTCA GAGTGTGGGA TTCAGTTAGT CTTTCTACAC TGCAAATTAT TGGACTTGGA
    ACTTTTGAAC GTGGAGTAGG ATGCCTGGAT TTTTCAAAAG CAGATTCAGG TGTTCATTTA
    TGTGTTATCG ATGACTCCAA TGAGCATATG CTTACTGTGT GGGATTGGCA GAAAAAATCA
    AAAGGAGCAG AAATAAAGAC AACAAATGAA GTTGTTTTGG CTGTGGGGTT CCATCCAACA
    GATGCAAATA TCATAATAAC ATGTGGTAAA TCACATATTT TTTTCTGGAC CTGGAGTGGC
    AATTCACTAA CAAAAAAACA AGGAGTTTTT GGGAAATACG AAAAACCAAA ATTTGTGCAG
    TGTTTAGCTT TCTTGGGAAA TGGAGATGTT CTTGCTGGAG ACTCAGGTGG AGTCATACTC
    ATATGGAGCA AAACTACTGT AGAGCCCACA CCTGGGAAAG GGCCTAAAGG TGTTTATCAG
    ATCAGCAAAC AAATCAAAGC TCATGATGGC AGTGTGTTCA CACTTTGTCA GATGAGAAAT
    GGGATGTTAT TAACTGGAGG TGGGAAAGAC AGAAAAATAA TTCTGTGGGA TCATGATCTG
    AATCCTGAAA GAGAAATAGA GGTTCCTGAT CAGTATGGAA CAATCAGAGC TGTAGCAGAA
    GGAAAGGCAG ATCAATTTTT GGTCGGCACA TCACGAAACT TTATTTTACG AGGAACATTT
    AATGATGGCT TCCAAATAGA AGTACAGGGT CATACAGATG AGCTTTGGGG TCTTGCCACA
    CATCCCTTCA AAGACTTGCT CTTGACTTGT GCTCAGGACA GACAGGTGTG CATGTGGAAC
    TCAGTAGAAC ACAGACTGGA ATGGACCAGG CTTCTAGATG AACCAGGACA CTGTGCAGAT
    TTTCATCCAA GTGGCACAGT GGTGGCCATA GGAACGCACT CAGGCAGGTG GTTTGTTCTG
    GATGCAGAAA CCAGAGATCT AGTTTCTATC CACACAGACG GAAATGAGCA GCTTTCGGTG
    ATGCGCTACT CAATAGATGG CACCCTCCTG GCTGTAGGAT CTCATGACAA CTTCATTTAC
    CTCTATGCCG TCTCAGAAAA TGGAAGAAAG TATAGCAGAC ATGGAAAGTG CACTGGACAT
    TCCAGCTATA TCACACACCT TGACTGGTCC CCAGACAACA AGTATATAAT GTCTAACTCG
    GGAGACTATG AGATCCTGTA CTGGGACATT GAACATGGCT GCAAACTAAT GAGGAATCGA
    TCAGAATGTA AGGACATCAA TTGGACGACA TACACCTGTG TGCTAGGCTT CCAAGTCTTT
    GGTGTCTGGC CAGAAGGATC TGATGGGACA GATATCAACG CGCTGGTACG ATCCCACAAC
    AGAAAGGTGA TCGCTGTTGC TGATGACTTT TGCAAAGTCC ATCTGTTTCA GTATCCCTGC
    TCCAAAGCAA AGGCTCCCAG TCACAAGTAC AGTGCCCACA GCAGCCATGT CACCAATGTC
    AGTTTTACTC ATAATGACAG TCACCTGATT TCAACTGGTG GAAAAGACAT GAGCATCATT
    CAGTGGAAAC TTGTGGAAAA GTTACCTTTG CCTCAAAATG AGACTCTAGC GGATCCTACA
    CTCACCAAAG CCCCTCCCTC TTCCATCGAG AGTGTGGTGC AGTCTGATAC CCCCACTCCG
    CCTCCTTCCC AGCCCTTAAA TGAGACAGCT GAAGAAGAAA GTCGGATAAG CAGTTCTCCC
    ACACTTCTGG AGAACAGCTT GGAACAGACC GTGGAGCCCA GCGAAGACCA CAGTGAGGAT
    CAGAGTGAGG GGGGCAGCGA AGACCTCGGT GAGCCTGTTT ATGAAGAGCT AGCCAATGAG
    CTAAGCGAGG AGCAGAGCAA AGCCGCCCTT CCCGGGGACC AGCAAGACCC CTCATCCCTG
    TCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa220581
    Clone ID Related Accession (Same CDS sequence) XM_024999056.1
    Accession Version XM_024999056.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2742bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product echinoderm microtubule-associated protein-like 4 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGTCCTGTA TAACCAACGG GAGTGGTACA AACAGAAAAC CAAGTCACAC CAGCTCTGTC 
    TCAATCGCAA GAAAAGAAAC TCTTTCATCT GCTGCTAAAA GTGGTACAGA AAAAAAGAAA
    GAAAAACCAC AAGGACAGAG AGAGAAAAAA GAGGACTCTC ACTCTAATGA TCAAAGTCCA
    CAAATTCGAG CATCACCTTC TCCCCAGCCC TCTTCACAGC CTCTCCAAAT ACACAGGCAA
    ACTCAAGAAA GCAAGAATTC TACTCCCACC AAAAGCATAA AACGACCATC AACAGCTGAA
    AAATCACATA ATTCATGGGA AAATTCAGAC GATAGCCGTA ATAAATTATT GAAGGCAGTT
    TCAACATCAA AATTAATATC AAAAGTTATA AAAAATACAG ACAAGCATAA AGATGTCATC
    ATCAACCAAG CAAAAATGTC AACCCGTGAA AAAAACAGCC AAGAAGGAGA ATACATTAAA
    ATGTTTATGC GAGGTCGACC AATTACAATG TTCATTCCTT CTGATGTTGA GAACTATGAT
    GACATCAGAA CAGAACTGCC TCCTGAGAAG CTCAAACTGG AGTGGGTATA CGGTTATCGA
    GGAAAGGATT GTCGAGCTAA TGTTTACCTT CTTCCTACCG GGGAAATTGT TTATTTCATA
    GCATCCGTAG TAGTACTGTT TAATTATGAA GAGAGAACTC AACGGCACTA CCTGGGTCAT
    ACAGACTGTG TAAAATGCCT TGCTATACAT CCTGACAAAA TTAGGATTGC AACTGGACAG
    ATAGCTGGAG TGGATAAGGA TGGAAGGCCC CTGCAACCCC ATGTCAGAGT GTGGGATTCA
    GTTAGTCTTT CTACACTGCA AATTATTGGA CTTGGAACTT TTGAACGTGG AGTAGGATGC
    CTGGATTTTT CAAAAGCAGA TTCAGGTGTT CATTTATGTG TTATCGATGA CTCCAATGAG
    CATATGCTTA CTGTGTGGGA TTGGCAGAAA AAATCAAAAG GAGCAGAAAT AAAGACAACA
    AATGAAGTTG TTTTGGCTGT GGGGTTCCAT CCAACAGATG CAAATATCAT AATAACATGT
    GGTAAATCAC ATATTTTTTT CTGGACCTGG AGTGGCAATT CACTAACAAA AAAACAAGGA
    GTTTTTGGGA AATACGAAAA ACCAAAATTT GTGCAGTGTT TAGCTTTCTT GGGAAATGGA
    GATGTTCTTG CTGGAGACTC AGGTGGAGTC ATACTCATAT GGAGCAAAAC TACTGTAGAG
    CCCACACCTG GGAAAGGGCC TAAAGGTGTT TATCAGATCA GCAAACAAAT CAAAGCTCAT
    GATGGCAGTG TGTTCACACT TTGTCAGATG AGAAATGGGA TGTTATTAAC TGGAGGTGGG
    AAAGACAGAA AAATAATTCT GTGGGATCAT GATCTGAATC CTGAAAGAGA AATAGAGGTT
    CCTGATCAGT ATGGAACAAT CAGAGCTGTA GCAGAAGGAA AGGCAGATCA ATTTTTGGTC
    GGCACATCAC GAAACTTTAT TTTACGAGGA ACATTTAATG ATGGCTTCCA AATAGAAGTA
    CAGGGTCATA CAGATGAGCT TTGGGGTCTT GCCACACATC CCTTCAAAGA CTTGCTCTTG
    ACTTGTGCTC AGGACAGACA GGTGTGCATG TGGAACTCAG TAGAACACAG ACTGGAATGG
    ACCAGGCTTC TAGATGAACC AGGACACTGT GCAGATTTTC ATCCAAGTGG CACAGTGGTG
    GCCATAGGAA CGCACTCAGG CAGGTGGTTT GTTCTGGATG CAGAAACCAG AGATCTAGTT
    TCTATCCACA CAGACGGAAA TGAGCAGCTT TCGGTGATGC GCTACTCAAT AGATGGCACC
    CTCCTGGCTG TAGGATCTCA TGACAACTTC ATTTACCTCT ATGCCGTCTC AGAAAATGGA
    AGAAAGTATA GCAGACATGG AAAGTGCACT GGACATTCCA GCTATATCAC ACACCTTGAC
    TGGTCCCCAG ACAACAAGTA TATAATGTCT AACTCGGGAG ACTATGAGAT CCTGTACTGG
    GACATTGAAC ATGGCTGCAA ACTAATGAGG AATCGATCAG AATGTAAGGA CATCAATTGG
    ACGACATACA CCTGTGTGCT AGGCTTCCAA GTCTTTGGTG TCTGGCCAGA AGGATCTGAT
    GGGACAGATA TCAACGCGCT GGTACGATCC CACAACAGAA AGGTGATCGC TGTTGCTGAT
    GACTTTTGCA AAGTCCATCT GTTTCAGTAT CCCTGCTCCA AAGCAAAGGC TCCCAGTCAC
    AAGTACAGTG CCCACAGCAG CCATGTCACC AATGTCAGTT TTACTCATAA TGACAGTCAC
    CTGATTTCAA CTGGTGGAAA AGACATGAGC ATCATTCAGT GGAAACTTGT GGAAAAGTTA
    CCTTTGCCTC AAAATGAGAC TCTAGCGGAT CCTACACTCA CCAAAGCCCC TCCCTCTTCC
    ATCGAGAGTG TGGTGCAGTC TGATACCCCC ACTCCGCCTC CTTCCCAGCC CTTAAATGAG
    ACAGCTGAAG AAGAAAGTCG GATAAGCAGT TCTCCCACAC TTCTGGAGAA CAGCTTGGAA
    CAGACCGTGG AGCCCAGCGA AGACCACAGT GAGGATCAGA GTGAGGGGGG CAGCGAAGAC
    CTCGGTGAGC CTGTTTATGA AGAGCTAGCC AATGAGCTAA GCGAGGAGCA GAGCAAAGCC
    GCCCTTCCCG GGGACCAGCA AGACCCCTCA TCCCTGTCCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024854824.1
    CDS424..3165
    Translation

    Target ORF information:

    RefSeq Version XM_024999056.1
    Organism Bos taurus(cattle)
    Definition Bos taurus echinoderm microtubule associated protein like 4 (EML4), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024999056.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGTCCTGTA TAACCAACGG GAGTGGTACA AACAGAAAAC CAAGTCACAC CAGCTCTGTC 
    TCAATCGCAA GAAAAGAAAC TCTTTCATCT GCTGCTAAAA GTGGTACAGA AAAAAAGAAA
    GAAAAACCAC AAGGACAGAG AGAGAAAAAA GAGGACTCTC ACTCTAATGA TCAAAGTCCA
    CAAATTCGAG CATCACCTTC TCCCCAGCCC TCTTCACAGC CTCTCCAAAT ACACAGGCAA
    ACTCAAGAAA GCAAGAATTC TACTCCCACC AAAAGCATAA AACGACCATC AACAGCTGAA
    AAATCACATA ATTCATGGGA AAATTCAGAC GATAGCCGTA ATAAATTATT GAAGGCAGTT
    TCAACATCAA AATTAATATC AAAAGTTATA AAAAATACAG ACAAGCATAA AGATGTCATC
    ATCAACCAAG CAAAAATGTC AACCCGTGAA AAAAACAGCC AAGAAGGAGA ATACATTAAA
    ATGTTTATGC GAGGTCGACC AATTACAATG TTCATTCCTT CTGATGTTGA GAACTATGAT
    GACATCAGAA CAGAACTGCC TCCTGAGAAG CTCAAACTGG AGTGGGTATA CGGTTATCGA
    GGAAAGGATT GTCGAGCTAA TGTTTACCTT CTTCCTACCG GGGAAATTGT TTATTTCATA
    GCATCCGTAG TAGTACTGTT TAATTATGAA GAGAGAACTC AACGGCACTA CCTGGGTCAT
    ACAGACTGTG TAAAATGCCT TGCTATACAT CCTGACAAAA TTAGGATTGC AACTGGACAG
    ATAGCTGGAG TGGATAAGGA TGGAAGGCCC CTGCAACCCC ATGTCAGAGT GTGGGATTCA
    GTTAGTCTTT CTACACTGCA AATTATTGGA CTTGGAACTT TTGAACGTGG AGTAGGATGC
    CTGGATTTTT CAAAAGCAGA TTCAGGTGTT CATTTATGTG TTATCGATGA CTCCAATGAG
    CATATGCTTA CTGTGTGGGA TTGGCAGAAA AAATCAAAAG GAGCAGAAAT AAAGACAACA
    AATGAAGTTG TTTTGGCTGT GGGGTTCCAT CCAACAGATG CAAATATCAT AATAACATGT
    GGTAAATCAC ATATTTTTTT CTGGACCTGG AGTGGCAATT CACTAACAAA AAAACAAGGA
    GTTTTTGGGA AATACGAAAA ACCAAAATTT GTGCAGTGTT TAGCTTTCTT GGGAAATGGA
    GATGTTCTTG CTGGAGACTC AGGTGGAGTC ATACTCATAT GGAGCAAAAC TACTGTAGAG
    CCCACACCTG GGAAAGGGCC TAAAGGTGTT TATCAGATCA GCAAACAAAT CAAAGCTCAT
    GATGGCAGTG TGTTCACACT TTGTCAGATG AGAAATGGGA TGTTATTAAC TGGAGGTGGG
    AAAGACAGAA AAATAATTCT GTGGGATCAT GATCTGAATC CTGAAAGAGA AATAGAGGTT
    CCTGATCAGT ATGGAACAAT CAGAGCTGTA GCAGAAGGAA AGGCAGATCA ATTTTTGGTC
    GGCACATCAC GAAACTTTAT TTTACGAGGA ACATTTAATG ATGGCTTCCA AATAGAAGTA
    CAGGGTCATA CAGATGAGCT TTGGGGTCTT GCCACACATC CCTTCAAAGA CTTGCTCTTG
    ACTTGTGCTC AGGACAGACA GGTGTGCATG TGGAACTCAG TAGAACACAG ACTGGAATGG
    ACCAGGCTTC TAGATGAACC AGGACACTGT GCAGATTTTC ATCCAAGTGG CACAGTGGTG
    GCCATAGGAA CGCACTCAGG CAGGTGGTTT GTTCTGGATG CAGAAACCAG AGATCTAGTT
    TCTATCCACA CAGACGGAAA TGAGCAGCTT TCGGTGATGC GCTACTCAAT AGATGGCACC
    CTCCTGGCTG TAGGATCTCA TGACAACTTC ATTTACCTCT ATGCCGTCTC AGAAAATGGA
    AGAAAGTATA GCAGACATGG AAAGTGCACT GGACATTCCA GCTATATCAC ACACCTTGAC
    TGGTCCCCAG ACAACAAGTA TATAATGTCT AACTCGGGAG ACTATGAGAT CCTGTACTGG
    GACATTGAAC ATGGCTGCAA ACTAATGAGG AATCGATCAG AATGTAAGGA CATCAATTGG
    ACGACATACA CCTGTGTGCT AGGCTTCCAA GTCTTTGGTG TCTGGCCAGA AGGATCTGAT
    GGGACAGATA TCAACGCGCT GGTACGATCC CACAACAGAA AGGTGATCGC TGTTGCTGAT
    GACTTTTGCA AAGTCCATCT GTTTCAGTAT CCCTGCTCCA AAGCAAAGGC TCCCAGTCAC
    AAGTACAGTG CCCACAGCAG CCATGTCACC AATGTCAGTT TTACTCATAA TGACAGTCAC
    CTGATTTCAA CTGGTGGAAA AGACATGAGC ATCATTCAGT GGAAACTTGT GGAAAAGTTA
    CCTTTGCCTC AAAATGAGAC TCTAGCGGAT CCTACACTCA CCAAAGCCCC TCCCTCTTCC
    ATCGAGAGTG TGGTGCAGTC TGATACCCCC ACTCCGCCTC CTTCCCAGCC CTTAAATGAG
    ACAGCTGAAG AAGAAAGTCG GATAAGCAGT TCTCCCACAC TTCTGGAGAA CAGCTTGGAA
    CAGACCGTGG AGCCCAGCGA AGACCACAGT GAGGATCAGA GTGAGGGGGG CAGCGAAGAC
    CTCGGTGAGC CTGTTTATGA AGAGCTAGCC AATGAGCTAA GCGAGGAGCA GAGCAAAGCC
    GCCCTTCCCG GGGACCAGCA AGACCCCTCA TCCCTGTCCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa54582
    Clone ID Related Accession (Same CDS sequence) XM_024999051.1
    Accession Version XM_024999051.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2997bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product echinoderm microtubule-associated protein-like 4 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGCTATGGA GCTCTCCAGA CAGCTGTCTG TGGGTGGTGG TGAGAGTGGA GGCGAACAGT 
    GGGTTGTGTG ACCAGGATGA TAGTATTTCC GCTGCAAGTA CTTCTGATGT TCAGGATCGC
    CTCTCAGCCC TTGAGTTACG AGTTCAACAG CAAGAAGATG AAATCACAGT GCTAAAGGCA
    GCTTTGGCTG ATGTTTTGAG GCGTCTTGCC ATCTCTGAAG ATCATGTAGC CTCGGTGAAA
    AAATCAGTCC CAAGTAAAGG CCAGCCAGGC CTTCGAGAAG CTATTTCTAT GTCCTGTATA
    ACCAACGGGA GTGGTACAAA CAGAAAACCA AGTCACACCA GCTCTGTCTC AATCGCAAGA
    AAAGAAACTC TTTCATCTGC TGCTAAAAGT GGTACAGAAA AAAAGAAAGA AAAACCACAA
    GGACAGAGAG AGAAAAAAGA GGACTCTCAC TCTAATGATC AAAGTCCACA AATTCGAGCA
    TCACCTTCTC CCCAGCCCTC TTCACAGCCT CTCCAAATAC ACAGGCAAAC TCAAGAAAGC
    AAGAATTCTA CTCCCACCAA AAGCATAAAA CGACCATCAA CAGCTGAAAA ATCACATAAT
    TCATGGGAAA ATTCAGACGA TAGCCGTAAT AAATTATTGA AGGCAGTTTC AACATCAAAA
    TTAATATCAA AAGTTATAAA AAATACAGAC AAGCATAAAG ATGTCATCAT CAACCAAGAA
    GGAGAATACA TTAAAATGTT TATGCGAGGT CGACCAATTA CAATGTTCAT TCCTTCTGAT
    GTTGAGAACT ATGATGACAT CAGAACAGAA CTGCCTCCTG AGAAGCTCAA ACTGGAGTGG
    GTATACGGTT ATCGAGGAAA GGATTGTCGA GCTAATGTTT ACCTTCTTCC TACCGGGGAA
    ATTGTTTATT TCATAGCATC CGTAGTAGTA CTGTTTAATT ATGAAGAGAG AACTCAACGG
    CACTACCTGG GTCATACAGA CTGTGTAAAA TGCCTTGCTA TACATCCTGA CAAAATTAGG
    ATTGCAACTG GACAGATAGC TGGAGTGGAT AAGGATGGAA GGCCCCTGCA ACCCCATGTC
    AGAGTGTGGG ATTCAGTTAG TCTTTCTACA CTGCAAATTA TTGGACTTGG AACTTTTGAA
    CGTGGAGTAG GATGCCTGGA TTTTTCAAAA GCAGATTCAG GTGTTCATTT ATGTGTTATC
    GATGACTCCA ATGAGCATAT GCTTACTGTG TGGGATTGGC AGAAAAAATC AAAAGGAGCA
    GAAATAAAGA CAACAAATGA AGTTGTTTTG GCTGTGGGGT TCCATCCAAC AGATGCAAAT
    ATCATAATAA CATGTGGTAA ATCACATATT TTTTTCTGGA CCTGGAGTGG CAATTCACTA
    ACAAAAAAAC AAGGAGTTTT TGGGAAATAC GAAAAACCAA AATTTGTGCA GTGTTTAGCT
    TTCTTGGGAA ATGGAGATGT TCTTGCTGGA GACTCAGGTG GAGTCATACT CATATGGAGC
    AAAACTACTG TAGAGCCCAC ACCTGGGAAA GGGCCTAAAG GTGTTTATCA GATCAGCAAA
    CAAATCAAAG CTCATGATGG CAGTGTGTTC ACACTTTGTC AGATGAGAAA TGGGATGTTA
    TTAACTGGAG GTGGGAAAGA CAGAAAAATA ATTCTGTGGG ATCATGATCT GAATCCTGAA
    AGAGAAATAG AGGTTCCTGA TCAGTATGGA ACAATCAGAG CTGTAGCAGA AGGAAAGGCA
    GATCAATTTT TGGTCGGCAC ATCACGAAAC TTTATTTTAC GAGGAACATT TAATGATGGC
    TTCCAAATAG AAGTACAGGG TCATACAGAT GAGCTTTGGG GTCTTGCCAC ACATCCCTTC
    AAAGACTTGC TCTTGACTTG TGCTCAGGAC AGACAGGTGT GCATGTGGAA CTCAGTAGAA
    CACAGACTGG AATGGACCAG GCTTCTAGAT GAACCAGGAC ACTGTGCAGA TTTTCATCCA
    AGTGGCACAG TGGTGGCCAT AGGAACGCAC TCAGGCAGGT GGTTTGTTCT GGATGCAGAA
    ACCAGAGATC TAGTTTCTAT CCACACAGAC GGAAATGAGC AGCTTTCGGT GATGCGCTAC
    TCAATAGATG GCACCCTCCT GGCTGTAGGA TCTCATGACA ACTTCATTTA CCTCTATGCC
    GTCTCAGAAA ATGGAAGAAA GTATAGCAGA CATGGAAAGT GCACTGGACA TTCCAGCTAT
    ATCACACACC TTGACTGGTC CCCAGACAAC AAGTATATAA TGTCTAACTC GGGAGACTAT
    GAGATCCTGT ACTGGGACAT TGAACATGGC TGCAAACTAA TGAGGAATCG ATCAGAATGT
    AAGGACATCA ATTGGACGAC ATACACCTGT GTGCTAGGCT TCCAAGTCTT TGGTGTCTGG
    CCAGAAGGAT CTGATGGGAC AGATATCAAC GCGCTGGTAC GATCCCACAA CAGAAAGGTG
    ATCGCTGTTG CTGATGACTT TTGCAAAGTC CATCTGTTTC AGTATCCCTG CTCCAAAGCA
    AAGGCTCCCA GTCACAAGTA CAGTGCCCAC AGCAGCCATG TCACCAATGT CAGTTTTACT
    CATAATGACA GTCACCTGAT TTCAACTGGT GGAAAAGACA TGAGCATCAT TCAGTGGAAA
    CTTGTGGAAA AGTTACCTTT GCCTCAAAAT GAGACTCTAG CGGATCCTAC ACTCACCAAA
    GCCCCTCCCT CTTCCATCGA GAGTGTGGTG CAGTCTGATA CCCCCACTCC GCCTCCTTCC
    CAGCCCTTAA ATGAGACAGC TGAAGAAGAA AGTCGGATAA GCAGTTCTCC CACACTTCTG
    GAGAACAGCT TGGAACAGAC CGTGGAGCCC AGCGAAGACC ACAGTGAGGA TCAGAGTGAG
    GGGGGCAGCG AAGACCTCGG TGAGCCTGTT TATGAAGAGC TAGCCAATGA GCTAAGCGAG
    GAGCAGAGCA AAGCCGCCCT TCCCGGGGAC CAGCAAGACC CCTCATCCCT GTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024854819.1
    CDS16..3012
    Translation

    Target ORF information:

    RefSeq Version XM_024999051.1
    Organism Bos taurus(cattle)
    Definition Bos taurus echinoderm microtubule associated protein like 4 (EML4), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024999051.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGCTATGGA GCTCTCCAGA CAGCTGTCTG TGGGTGGTGG TGAGAGTGGA GGCGAACAGT 
    GGGTTGTGTG ACCAGGATGA TAGTATTTCC GCTGCAAGTA CTTCTGATGT TCAGGATCGC
    CTCTCAGCCC TTGAGTTACG AGTTCAACAG CAAGAAGATG AAATCACAGT GCTAAAGGCA
    GCTTTGGCTG ATGTTTTGAG GCGTCTTGCC ATCTCTGAAG ATCATGTAGC CTCGGTGAAA
    AAATCAGTCC CAAGTAAAGG CCAGCCAGGC CTTCGAGAAG CTATTTCTAT GTCCTGTATA
    ACCAACGGGA GTGGTACAAA CAGAAAACCA AGTCACACCA GCTCTGTCTC AATCGCAAGA
    AAAGAAACTC TTTCATCTGC TGCTAAAAGT GGTACAGAAA AAAAGAAAGA AAAACCACAA
    GGACAGAGAG AGAAAAAAGA GGACTCTCAC TCTAATGATC AAAGTCCACA AATTCGAGCA
    TCACCTTCTC CCCAGCCCTC TTCACAGCCT CTCCAAATAC ACAGGCAAAC TCAAGAAAGC
    AAGAATTCTA CTCCCACCAA AAGCATAAAA CGACCATCAA CAGCTGAAAA ATCACATAAT
    TCATGGGAAA ATTCAGACGA TAGCCGTAAT AAATTATTGA AGGCAGTTTC AACATCAAAA
    TTAATATCAA AAGTTATAAA AAATACAGAC AAGCATAAAG ATGTCATCAT CAACCAAGAA
    GGAGAATACA TTAAAATGTT TATGCGAGGT CGACCAATTA CAATGTTCAT TCCTTCTGAT
    GTTGAGAACT ATGATGACAT CAGAACAGAA CTGCCTCCTG AGAAGCTCAA ACTGGAGTGG
    GTATACGGTT ATCGAGGAAA GGATTGTCGA GCTAATGTTT ACCTTCTTCC TACCGGGGAA
    ATTGTTTATT TCATAGCATC CGTAGTAGTA CTGTTTAATT ATGAAGAGAG AACTCAACGG
    CACTACCTGG GTCATACAGA CTGTGTAAAA TGCCTTGCTA TACATCCTGA CAAAATTAGG
    ATTGCAACTG GACAGATAGC TGGAGTGGAT AAGGATGGAA GGCCCCTGCA ACCCCATGTC
    AGAGTGTGGG ATTCAGTTAG TCTTTCTACA CTGCAAATTA TTGGACTTGG AACTTTTGAA
    CGTGGAGTAG GATGCCTGGA TTTTTCAAAA GCAGATTCAG GTGTTCATTT ATGTGTTATC
    GATGACTCCA ATGAGCATAT GCTTACTGTG TGGGATTGGC AGAAAAAATC AAAAGGAGCA
    GAAATAAAGA CAACAAATGA AGTTGTTTTG GCTGTGGGGT TCCATCCAAC AGATGCAAAT
    ATCATAATAA CATGTGGTAA ATCACATATT TTTTTCTGGA CCTGGAGTGG CAATTCACTA
    ACAAAAAAAC AAGGAGTTTT TGGGAAATAC GAAAAACCAA AATTTGTGCA GTGTTTAGCT
    TTCTTGGGAA ATGGAGATGT TCTTGCTGGA GACTCAGGTG GAGTCATACT CATATGGAGC
    AAAACTACTG TAGAGCCCAC ACCTGGGAAA GGGCCTAAAG GTGTTTATCA GATCAGCAAA
    CAAATCAAAG CTCATGATGG CAGTGTGTTC ACACTTTGTC AGATGAGAAA TGGGATGTTA
    TTAACTGGAG GTGGGAAAGA CAGAAAAATA ATTCTGTGGG ATCATGATCT GAATCCTGAA
    AGAGAAATAG AGGTTCCTGA TCAGTATGGA ACAATCAGAG CTGTAGCAGA AGGAAAGGCA
    GATCAATTTT TGGTCGGCAC ATCACGAAAC TTTATTTTAC GAGGAACATT TAATGATGGC
    TTCCAAATAG AAGTACAGGG TCATACAGAT GAGCTTTGGG GTCTTGCCAC ACATCCCTTC
    AAAGACTTGC TCTTGACTTG TGCTCAGGAC AGACAGGTGT GCATGTGGAA CTCAGTAGAA
    CACAGACTGG AATGGACCAG GCTTCTAGAT GAACCAGGAC ACTGTGCAGA TTTTCATCCA
    AGTGGCACAG TGGTGGCCAT AGGAACGCAC TCAGGCAGGT GGTTTGTTCT GGATGCAGAA
    ACCAGAGATC TAGTTTCTAT CCACACAGAC GGAAATGAGC AGCTTTCGGT GATGCGCTAC
    TCAATAGATG GCACCCTCCT GGCTGTAGGA TCTCATGACA ACTTCATTTA CCTCTATGCC
    GTCTCAGAAA ATGGAAGAAA GTATAGCAGA CATGGAAAGT GCACTGGACA TTCCAGCTAT
    ATCACACACC TTGACTGGTC CCCAGACAAC AAGTATATAA TGTCTAACTC GGGAGACTAT
    GAGATCCTGT ACTGGGACAT TGAACATGGC TGCAAACTAA TGAGGAATCG ATCAGAATGT
    AAGGACATCA ATTGGACGAC ATACACCTGT GTGCTAGGCT TCCAAGTCTT TGGTGTCTGG
    CCAGAAGGAT CTGATGGGAC AGATATCAAC GCGCTGGTAC GATCCCACAA CAGAAAGGTG
    ATCGCTGTTG CTGATGACTT TTGCAAAGTC CATCTGTTTC AGTATCCCTG CTCCAAAGCA
    AAGGCTCCCA GTCACAAGTA CAGTGCCCAC AGCAGCCATG TCACCAATGT CAGTTTTACT
    CATAATGACA GTCACCTGAT TTCAACTGGT GGAAAAGACA TGAGCATCAT TCAGTGGAAA
    CTTGTGGAAA AGTTACCTTT GCCTCAAAAT GAGACTCTAG CGGATCCTAC ACTCACCAAA
    GCCCCTCCCT CTTCCATCGA GAGTGTGGTG CAGTCTGATA CCCCCACTCC GCCTCCTTCC
    CAGCCCTTAA ATGAGACAGC TGAAGAAGAA AGTCGGATAA GCAGTTCTCC CACACTTCTG
    GAGAACAGCT TGGAACAGAC CGTGGAGCCC AGCGAAGACC ACAGTGAGGA TCAGAGTGAG
    GGGGGCAGCG AAGACCTCGG TGAGCCTGTT TATGAAGAGC TAGCCAATGA GCTAAGCGAG
    GAGCAGAGCA AAGCCGCCCT TCCCGGGGAC CAGCAAGACC CCTCATCCCT GTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa54583
    Clone ID Related Accession (Same CDS sequence) XM_024999052.1
    Accession Version XM_024999052.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2979bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product echinoderm microtubule-associated protein-like 4 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGACGGTT TCGCCGGCAG CCTCGATGAT AGTATTTCCG CTGCAAGTAC TTCTGATGTT 
    CAGGATCGCC TCTCAGCCCT TGAGTTACGA GTTCAACAGC AAGAAGATGA AATCACAGTG
    CTAAAGGCAG CTTTGGCTGA TGTTTTGAGG CGTCTTGCCA TCTCTGAAGA TCATGTAGCC
    TCGGTGAAAA AATCAGTCCC AAGTAAAGGC CAGCCAGGCC TTCGAGAAGC TATTTCTATG
    TCCTGTATAA CCAACGGGAG TGGTACAAAC AGAAAACCAA GTCACACCAG CTCTGTCTCA
    ATCGCAAGAA AAGAAACTCT TTCATCTGCT GCTAAAAGTG GTACAGAAAA AAAGAAAGAA
    AAACCACAAG GACAGAGAGA GAAAAAAGAG GACTCTCACT CTAATGATCA AAGTCCACAA
    ATTCGAGCAT CACCTTCTCC CCAGCCCTCT TCACAGCCTC TCCAAATACA CAGGCAAACT
    CAAGAAAGCA AGAATTCTAC TCCCACCAAA AGCATAAAAC GACCATCAAC AGCTGAAAAA
    TCACATAATT CATGGGAAAA TTCAGACGAT AGCCGTAATA AATTATTGAA GGCAGTTTCA
    ACATCAAAAT TAATATCAAA AGTTATAAAA AATACAGACA AGCATAAAGA TGTCATCATC
    AACCAAGCAA AAATGTCAAC CCGTGAAAAA AACAGCCAAG AAGGAGAATA CATTAAAATG
    TTTATGCGAG GTCGACCAAT TACAATGTTC ATTCCTTCTG ATGTTGAGAA CTATGATGAC
    ATCAGAACAG AACTGCCTCC TGAGAAGCTC AAACTGGAGT GGGTATACGG TTATCGAGGA
    AAGGATTGTC GAGCTAATGT TTACCTTCTT CCTACCGGGG AAATTGTTTA TTTCATAGCA
    TCCGTAGTAG TACTGTTTAA TTATGAAGAG AGAACTCAAC GGCACTACCT GGGTCATACA
    GACTGTGTAA AATGCCTTGC TATACATCCT GACAAAATTA GGATTGCAAC TGGACAGATA
    GCTGGAGTGG ATAAGGATGG AAGGCCCCTG CAACCCCATG TCAGAGTGTG GGATTCAGTT
    AGTCTTTCTA CACTGCAAAT TATTGGACTT GGAACTTTTG AACGTGGAGT AGGATGCCTG
    GATTTTTCAA AAGCAGATTC AGGTGTTCAT TTATGTGTTA TCGATGACTC CAATGAGCAT
    ATGCTTACTG TGTGGGATTG GCAGAAAAAA TCAAAAGGAG CAGAAATAAA GACAACAAAT
    GAAGTTGTTT TGGCTGTGGG GTTCCATCCA ACAGATGCAA ATATCATAAT AACATGTGGT
    AAATCACATA TTTTTTTCTG GACCTGGAGT GGCAATTCAC TAACAAAAAA ACAAGGAGTT
    TTTGGGAAAT ACGAAAAACC AAAATTTGTG CAGTGTTTAG CTTTCTTGGG AAATGGAGAT
    GTTCTTGCTG GAGACTCAGG TGGAGTCATA CTCATATGGA GCAAAACTAC TGTAGAGCCC
    ACACCTGGGA AAGGGCCTAA AGGTGTTTAT CAGATCAGCA AACAAATCAA AGCTCATGAT
    GGCAGTGTGT TCACACTTTG TCAGATGAGA AATGGGATGT TATTAACTGG AGGTGGGAAA
    GACAGAAAAA TAATTCTGTG GGATCATGAT CTGAATCCTG AAAGAGAAAT AGAGGTTCCT
    GATCAGTATG GAACAATCAG AGCTGTAGCA GAAGGAAAGG CAGATCAATT TTTGGTCGGC
    ACATCACGAA ACTTTATTTT ACGAGGAACA TTTAATGATG GCTTCCAAAT AGAAGTACAG
    GGTCATACAG ATGAGCTTTG GGGTCTTGCC ACACATCCCT TCAAAGACTT GCTCTTGACT
    TGTGCTCAGG ACAGACAGGT GTGCATGTGG AACTCAGTAG AACACAGACT GGAATGGACC
    AGGCTTCTAG ATGAACCAGG ACACTGTGCA GATTTTCATC CAAGTGGCAC AGTGGTGGCC
    ATAGGAACGC ACTCAGGCAG GTGGTTTGTT CTGGATGCAG AAACCAGAGA TCTAGTTTCT
    ATCCACACAG ACGGAAATGA GCAGCTTTCG GTGATGCGCT ACTCAATAGA TGGCACCCTC
    CTGGCTGTAG GATCTCATGA CAACTTCATT TACCTCTATG CCGTCTCAGA AAATGGAAGA
    AAGTATAGCA GACATGGAAA GTGCACTGGA CATTCCAGCT ATATCACACA CCTTGACTGG
    TCCCCAGACA ACAAGTATAT AATGTCTAAC TCGGGAGACT ATGAGATCCT GTACTGGGAC
    ATTGAACATG GCTGCAAACT AATGAGGAAT CGATCAGAAT GTAAGGACAT CAATTGGACG
    ACATACACCT GTGTGCTAGG CTTCCAAGTC TTTGGTGTCT GGCCAGAAGG ATCTGATGGG
    ACAGATATCA ACGCGCTGGT ACGATCCCAC AACAGAAAGG TGATCGCTGT TGCTGATGAC
    TTTTGCAAAG TCCATCTGTT TCAGTATCCC TGCTCCAAAG CAAAGGCTCC CAGTCACAAG
    TACAGTGCCC ACAGCAGCCA TGTCACCAAT GTCAGTTTTA CTCATAATGA CAGTCACCTG
    ATTTCAACTG GTGGAAAAGA CATGAGCATC ATTCAGTGGA AACTTGTGGA AAAGTTACCT
    TTGCCTCAAA ATGAGACTCT AGCGGATCCT ACACTCACCA AAGCCCCTCC CTCTTCCATC
    GAGAGTGTGG TGCAGTCTGA TACCCCCACT CCGCCTCCTT CCCAGCCCTT AAATGAGACA
    GCTGAAGAAG AAAGTCGGAT AAGCAGTTCT CCCACACTTC TGGAGAACAG CTTGGAACAG
    ACCGTGGAGC CCAGCGAAGA CCACAGTGAG GATCAGAGTG AGGGGGGCAG CGAAGACCTC
    GGTGAGCCTG TTTATGAAGA GCTAGCCAAT GAGCTAAGCG AGGAGCAGAG CAAAGCCGCC
    CTTCCCGGGG ACCAGCAAGA CCCCTCATCC CTGTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024854820.1
    CDS290..3268
    Translation

    Target ORF information:

    RefSeq Version XM_024999052.1
    Organism Bos taurus(cattle)
    Definition Bos taurus echinoderm microtubule associated protein like 4 (EML4), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024999052.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGACGGTT TCGCCGGCAG CCTCGATGAT AGTATTTCCG CTGCAAGTAC TTCTGATGTT 
    CAGGATCGCC TCTCAGCCCT TGAGTTACGA GTTCAACAGC AAGAAGATGA AATCACAGTG
    CTAAAGGCAG CTTTGGCTGA TGTTTTGAGG CGTCTTGCCA TCTCTGAAGA TCATGTAGCC
    TCGGTGAAAA AATCAGTCCC AAGTAAAGGC CAGCCAGGCC TTCGAGAAGC TATTTCTATG
    TCCTGTATAA CCAACGGGAG TGGTACAAAC AGAAAACCAA GTCACACCAG CTCTGTCTCA
    ATCGCAAGAA AAGAAACTCT TTCATCTGCT GCTAAAAGTG GTACAGAAAA AAAGAAAGAA
    AAACCACAAG GACAGAGAGA GAAAAAAGAG GACTCTCACT CTAATGATCA AAGTCCACAA
    ATTCGAGCAT CACCTTCTCC CCAGCCCTCT TCACAGCCTC TCCAAATACA CAGGCAAACT
    CAAGAAAGCA AGAATTCTAC TCCCACCAAA AGCATAAAAC GACCATCAAC AGCTGAAAAA
    TCACATAATT CATGGGAAAA TTCAGACGAT AGCCGTAATA AATTATTGAA GGCAGTTTCA
    ACATCAAAAT TAATATCAAA AGTTATAAAA AATACAGACA AGCATAAAGA TGTCATCATC
    AACCAAGCAA AAATGTCAAC CCGTGAAAAA AACAGCCAAG AAGGAGAATA CATTAAAATG
    TTTATGCGAG GTCGACCAAT TACAATGTTC ATTCCTTCTG ATGTTGAGAA CTATGATGAC
    ATCAGAACAG AACTGCCTCC TGAGAAGCTC AAACTGGAGT GGGTATACGG TTATCGAGGA
    AAGGATTGTC GAGCTAATGT TTACCTTCTT CCTACCGGGG AAATTGTTTA TTTCATAGCA
    TCCGTAGTAG TACTGTTTAA TTATGAAGAG AGAACTCAAC GGCACTACCT GGGTCATACA
    GACTGTGTAA AATGCCTTGC TATACATCCT GACAAAATTA GGATTGCAAC TGGACAGATA
    GCTGGAGTGG ATAAGGATGG AAGGCCCCTG CAACCCCATG TCAGAGTGTG GGATTCAGTT
    AGTCTTTCTA CACTGCAAAT TATTGGACTT GGAACTTTTG AACGTGGAGT AGGATGCCTG
    GATTTTTCAA AAGCAGATTC AGGTGTTCAT TTATGTGTTA TCGATGACTC CAATGAGCAT
    ATGCTTACTG TGTGGGATTG GCAGAAAAAA TCAAAAGGAG CAGAAATAAA GACAACAAAT
    GAAGTTGTTT TGGCTGTGGG GTTCCATCCA ACAGATGCAA ATATCATAAT AACATGTGGT
    AAATCACATA TTTTTTTCTG GACCTGGAGT GGCAATTCAC TAACAAAAAA ACAAGGAGTT
    TTTGGGAAAT ACGAAAAACC AAAATTTGTG CAGTGTTTAG CTTTCTTGGG AAATGGAGAT
    GTTCTTGCTG GAGACTCAGG TGGAGTCATA CTCATATGGA GCAAAACTAC TGTAGAGCCC
    ACACCTGGGA AAGGGCCTAA AGGTGTTTAT CAGATCAGCA AACAAATCAA AGCTCATGAT
    GGCAGTGTGT TCACACTTTG TCAGATGAGA AATGGGATGT TATTAACTGG AGGTGGGAAA
    GACAGAAAAA TAATTCTGTG GGATCATGAT CTGAATCCTG AAAGAGAAAT AGAGGTTCCT
    GATCAGTATG GAACAATCAG AGCTGTAGCA GAAGGAAAGG CAGATCAATT TTTGGTCGGC
    ACATCACGAA ACTTTATTTT ACGAGGAACA TTTAATGATG GCTTCCAAAT AGAAGTACAG
    GGTCATACAG ATGAGCTTTG GGGTCTTGCC ACACATCCCT TCAAAGACTT GCTCTTGACT
    TGTGCTCAGG ACAGACAGGT GTGCATGTGG AACTCAGTAG AACACAGACT GGAATGGACC
    AGGCTTCTAG ATGAACCAGG ACACTGTGCA GATTTTCATC CAAGTGGCAC AGTGGTGGCC
    ATAGGAACGC ACTCAGGCAG GTGGTTTGTT CTGGATGCAG AAACCAGAGA TCTAGTTTCT
    ATCCACACAG ACGGAAATGA GCAGCTTTCG GTGATGCGCT ACTCAATAGA TGGCACCCTC
    CTGGCTGTAG GATCTCATGA CAACTTCATT TACCTCTATG CCGTCTCAGA AAATGGAAGA
    AAGTATAGCA GACATGGAAA GTGCACTGGA CATTCCAGCT ATATCACACA CCTTGACTGG
    TCCCCAGACA ACAAGTATAT AATGTCTAAC TCGGGAGACT ATGAGATCCT GTACTGGGAC
    ATTGAACATG GCTGCAAACT AATGAGGAAT CGATCAGAAT GTAAGGACAT CAATTGGACG
    ACATACACCT GTGTGCTAGG CTTCCAAGTC TTTGGTGTCT GGCCAGAAGG ATCTGATGGG
    ACAGATATCA ACGCGCTGGT ACGATCCCAC AACAGAAAGG TGATCGCTGT TGCTGATGAC
    TTTTGCAAAG TCCATCTGTT TCAGTATCCC TGCTCCAAAG CAAAGGCTCC CAGTCACAAG
    TACAGTGCCC ACAGCAGCCA TGTCACCAAT GTCAGTTTTA CTCATAATGA CAGTCACCTG
    ATTTCAACTG GTGGAAAAGA CATGAGCATC ATTCAGTGGA AACTTGTGGA AAAGTTACCT
    TTGCCTCAAA ATGAGACTCT AGCGGATCCT ACACTCACCA AAGCCCCTCC CTCTTCCATC
    GAGAGTGTGG TGCAGTCTGA TACCCCCACT CCGCCTCCTT CCCAGCCCTT AAATGAGACA
    GCTGAAGAAG AAAGTCGGAT AAGCAGTTCT CCCACACTTC TGGAGAACAG CTTGGAACAG
    ACCGTGGAGC CCAGCGAAGA CCACAGTGAG GATCAGAGTG AGGGGGGCAG CGAAGACCTC
    GGTGAGCCTG TTTATGAAGA GCTAGCCAAT GAGCTAAGCG AGGAGCAGAG CAAAGCCGCC
    CTTCCCGGGG ACCAGCAAGA CCCCTCATCC CTGTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa91870
    Clone ID Related Accession (Same CDS sequence) XM_015473450.1
    Accession Version XM_015473450.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2871bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product echinoderm microtubule-associated protein-like 4 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104293.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGAAGACA GCCTGCCTAG TCTCGCTTCA GTATGTGCTT CCTCTACACT TTCAAACTTC 
    TGTACTGATG ATAGTATTTC CGCTGCAAGT ACTTCTGATG TTCAGGATCG CCTCTCAGCC
    CTTGAGTTAC GAGTTCAACA GCAAGAAGAT GAAATCACAG TGCTAAAGGC AGCTTTGGCT
    GATGTTTTGA GGCGTCTTGC CATCTCTGAA GATCATGTAG CCTCGGTGAA AAAATCAGTC
    CCAAGTAAAG GCCAGCCAGG CCTTCGAGAA GCTATTTCTA TGTCCTGTAT AACCAACGGG
    AGTGGTACAA ACAGAAAACC AAGTCACACC AGCTCTGTCT CAATCGCAAG AAAAGAAACT
    CTTTCATCTG CTGCTAAAAG CATAAAACGA CCATCAACAG CTGAAAAATC ACATAATTCA
    TGGGAAAATT CAGACGATAG CCGTAATAAA TTATTGAAGG CAGTTTCAAC ATCAAAATTA
    ATATCAAAAG TTATAAAAAA TACAGACAAG CATAAAGATG TCATCATCAA CCAAGATATG
    TCATTTTTGT CTCAACCAGC AAAAATGTCA ACCCGTGAAA AAAACAGCCA AGAAGGAGAA
    TACATTAAAA TGTTTATGCG AGGTCGACCA ATTACAATGT TCATTCCTTC TGATGTTGAG
    AACTATGATG ACATCAGAAC AGAACTGCCT CCTGAGAAGC TCAAACTGGA GTGGGTATAC
    GGTTATCGAG GAAAGGATTG TCGAGCTAAT GTTTACCTTC TTCCTACCGG GGAAATTGTT
    TATTTCATAG CATCCGTAGT AGTACTGTTT AATTATGAAG AGAGAACTCA ACGGCACTAC
    CTGGGTCATA CAGACTGTGT AAAATGCCTT GCTATACATC CTGACAAAAT TAGGATTGCA
    ACTGGACAGA TAGCTGGAGT GGATAAGGAT GGAAGGCCCC TGCAACCCCA TGTCAGAGTG
    TGGGATTCAG TTAGTCTTTC TACACTGCAA ATTATTGGAC TTGGAACTTT TGAACGTGGA
    GTAGGATGCC TGGATTTTTC AAAAGCAGAT TCAGGTGTTC ATTTATGTGT TATCGATGAC
    TCCAATGAGC ATATGCTTAC TGTGTGGGAT TGGCAGAAAA AATCAAAAGG AGCAGAAATA
    AAGACAACAA ATGAAGTTGT TTTGGCTGTG GGGTTCCATC CAACAGATGC AAATATCATA
    ATAACATGTG GTAAATCACA TATTTTTTTC TGGACCTGGA GTGGCAATTC ACTAACAAAA
    AAACAAGGAG TTTTTGGGAA ATACGAAAAA CCAAAATTTG TGCAGTGTTT AGCTTTCTTG
    GGAAATGGAG ATGTTCTTGC TGGAGACTCA GGTGGAGTCA TACTCATATG GAGCAAAACT
    ACTGTAGAGC CCACACCTGG GAAAGGGCCT AAAGGTGTTT ATCAGATCAG CAAACAAATC
    AAAGCTCATG ATGGCAGTGT GTTCACACTT TGTCAGATGA GAAATGGGAT GTTATTAACT
    GGAGGTGGGA AAGACAGAAA AATAATTCTG TGGGATCATG ATCTGAATCC TGAAAGAGAA
    ATAGAGGTTC CTGATCAGTA TGGAACAATC AGAGCTGTAG CAGAAGGAAA GGCAGATCAA
    TTTTTGGTCG GCACATCACG AAACTTTATT TTACGAGGAA CATTTAATGA TGGCTTCCAA
    ATAGAAGTAC AGGGTCATAC AGATGAGCTT TGGGGTCTTG CCACACATCC CTTCAAAGAC
    TTGCTCTTGA CTTGTGCTCA GGACAGACAG GTGTGCATGT GGAACTCAGT AGAACACAGA
    CTGGAATGGA CCAGGCTTCT AGATGAACCA GGACACTGTG CAGATTTTCA TCCAAGTGGC
    ACAGTGGTGG CCATAGGAAC GCACTCAGGC AGGTGGTTTG TTCTGGATGC AGAAACCAGA
    GATCTAGTTT CTATCCACAC AGACGGAAAT GAGCAGCTTT CGGTGATGCG CTACTCAATA
    GATGGCACCC TCCTGGCTGT AGGATCTCAT GACAACTTCA TTTACCTCTA TGCCGTCTCA
    GAAAATGGAA GAAAGTATAG CAGACATGGA AAGTGCACTG GACATTCCAG CTATATCACA
    CACCTTGACT GGTCCCCAGA CAACAAGTAT ATAATGTCTA ACTCGGGAGA CTATGAGATC
    CTGTACTGGG ACATTGAACA TGGCTGCAAA CTAATGAGGA ATCGATCAGA ATGTAAGGAC
    ATCAATTGGA CGACATACAC CTGTGTGCTA GGCTTCCAAG TCTTTGGTGT CTGGCCAGAA
    GGATCTGATG GGACAGATAT CAACGCGCTG GTACGATCCC ACAACAGAAA GGTGATCGCT
    GTTGCTGATG ACTTTTGCAA AGTCCATCTG TTTCAGTATC CCTGCTCCAA AGCAAAGGCT
    CCCAGTCACA AGTACAGTGC CCACAGCAGC CATGTCACCA ATGTCAGTTT TACTCATAAT
    GACAGTCACC TGATTTCAAC TGGTGGAAAA GACATGAGCA TCATTCAGTG GAAACTTGTG
    GAAAAGTTAC CTTTGCCTCA AAATGAGACT CTAGCGGATC CTACACTCAC CAAAGCCCCT
    CCCTCTTCCA TCGAGAGTGT GGTGCAGTCT GATACCCCCA CTCCGCCTCC TTCCCAGCCC
    TTAAATGAGA CAGCTGAAGA AGAAAGTCGG ATAAGCAGTT CTCCCACACT TCTGGAGAAC
    AGCTTGGAAC AGACCGTGGA GCCCAGCGAA GACCACAGTG AGGATCAGAG TGAGGGGGGC
    AGCGAAGACC TCGGTGAGCC TGTTTATGAA GAGCTAGCCA ATGAGCTAAG CGAGGAGCAG
    AGCAAAGCCG CCCTTCCCGG GGACCAGCAA GACCCCTCAT CCCTGTCCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015328936.1
    CDS55..2925
    Translation

    Target ORF information:

    RefSeq Version XM_015473450.1
    Organism Bos taurus(cattle)
    Definition Bos taurus echinoderm microtubule associated protein like 4 (EML4), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015473450.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGAAGACA GCCTGCCTAG TCTCGCTTCA GTATGTGCTT CCTCTACACT TTCAAACTTC 
    TGTACTGATG ATAGTATTTC CGCTGCAAGT ACTTCTGATG TTCAGGATCG CCTCTCAGCC
    CTTGAGTTAC GAGTTCAACA GCAAGAAGAT GAAATCACAG TGCTAAAGGC AGCTTTGGCT
    GATGTTTTGA GGCGTCTTGC CATCTCTGAA GATCATGTAG CCTCGGTGAA AAAATCAGTC
    CCAAGTAAAG GCCAGCCAGG CCTTCGAGAA GCTATTTCTA TGTCCTGTAT AACCAACGGG
    AGTGGTACAA ACAGAAAACC AAGTCACACC AGCTCTGTCT CAATCGCAAG AAAAGAAACT
    CTTTCATCTG CTGCTAAAAG CATAAAACGA CCATCAACAG CTGAAAAATC ACATAATTCA
    TGGGAAAATT CAGACGATAG CCGTAATAAA TTATTGAAGG CAGTTTCAAC ATCAAAATTA
    ATATCAAAAG TTATAAAAAA TACAGACAAG CATAAAGATG TCATCATCAA CCAAGATATG
    TCATTTTTGT CTCAACCAGC AAAAATGTCA ACCCGTGAAA AAAACAGCCA AGAAGGAGAA
    TACATTAAAA TGTTTATGCG AGGTCGACCA ATTACAATGT TCATTCCTTC TGATGTTGAG
    AACTATGATG ACATCAGAAC AGAACTGCCT CCTGAGAAGC TCAAACTGGA GTGGGTATAC
    GGTTATCGAG GAAAGGATTG TCGAGCTAAT GTTTACCTTC TTCCTACCGG GGAAATTGTT
    TATTTCATAG CATCCGTAGT AGTACTGTTT AATTATGAAG AGAGAACTCA ACGGCACTAC
    CTGGGTCATA CAGACTGTGT AAAATGCCTT GCTATACATC CTGACAAAAT TAGGATTGCA
    ACTGGACAGA TAGCTGGAGT GGATAAGGAT GGAAGGCCCC TGCAACCCCA TGTCAGAGTG
    TGGGATTCAG TTAGTCTTTC TACACTGCAA ATTATTGGAC TTGGAACTTT TGAACGTGGA
    GTAGGATGCC TGGATTTTTC AAAAGCAGAT TCAGGTGTTC ATTTATGTGT TATCGATGAC
    TCCAATGAGC ATATGCTTAC TGTGTGGGAT TGGCAGAAAA AATCAAAAGG AGCAGAAATA
    AAGACAACAA ATGAAGTTGT TTTGGCTGTG GGGTTCCATC CAACAGATGC AAATATCATA
    ATAACATGTG GTAAATCACA TATTTTTTTC TGGACCTGGA GTGGCAATTC ACTAACAAAA
    AAACAAGGAG TTTTTGGGAA ATACGAAAAA CCAAAATTTG TGCAGTGTTT AGCTTTCTTG
    GGAAATGGAG ATGTTCTTGC TGGAGACTCA GGTGGAGTCA TACTCATATG GAGCAAAACT
    ACTGTAGAGC CCACACCTGG GAAAGGGCCT AAAGGTGTTT ATCAGATCAG CAAACAAATC
    AAAGCTCATG ATGGCAGTGT GTTCACACTT TGTCAGATGA GAAATGGGAT GTTATTAACT
    GGAGGTGGGA AAGACAGAAA AATAATTCTG TGGGATCATG ATCTGAATCC TGAAAGAGAA
    ATAGAGGTTC CTGATCAGTA TGGAACAATC AGAGCTGTAG CAGAAGGAAA GGCAGATCAA
    TTTTTGGTCG GCACATCACG AAACTTTATT TTACGAGGAA CATTTAATGA TGGCTTCCAA
    ATAGAAGTAC AGGGTCATAC AGATGAGCTT TGGGGTCTTG CCACACATCC CTTCAAAGAC
    TTGCTCTTGA CTTGTGCTCA GGACAGACAG GTGTGCATGT GGAACTCAGT AGAACACAGA
    CTGGAATGGA CCAGGCTTCT AGATGAACCA GGACACTGTG CAGATTTTCA TCCAAGTGGC
    ACAGTGGTGG CCATAGGAAC GCACTCAGGC AGGTGGTTTG TTCTGGATGC AGAAACCAGA
    GATCTAGTTT CTATCCACAC AGACGGAAAT GAGCAGCTTT CGGTGATGCG CTACTCAATA
    GATGGCACCC TCCTGGCTGT AGGATCTCAT GACAACTTCA TTTACCTCTA TGCCGTCTCA
    GAAAATGGAA GAAAGTATAG CAGACATGGA AAGTGCACTG GACATTCCAG CTATATCACA
    CACCTTGACT GGTCCCCAGA CAACAAGTAT ATAATGTCTA ACTCGGGAGA CTATGAGATC
    CTGTACTGGG ACATTGAACA TGGCTGCAAA CTAATGAGGA ATCGATCAGA ATGTAAGGAC
    ATCAATTGGA CGACATACAC CTGTGTGCTA GGCTTCCAAG TCTTTGGTGT CTGGCCAGAA
    GGATCTGATG GGACAGATAT CAACGCGCTG GTACGATCCC ACAACAGAAA GGTGATCGCT
    GTTGCTGATG ACTTTTGCAA AGTCCATCTG TTTCAGTATC CCTGCTCCAA AGCAAAGGCT
    CCCAGTCACA AGTACAGTGC CCACAGCAGC CATGTCACCA ATGTCAGTTT TACTCATAAT
    GACAGTCACC TGATTTCAAC TGGTGGAAAA GACATGAGCA TCATTCAGTG GAAACTTGTG
    GAAAAGTTAC CTTTGCCTCA AAATGAGACT CTAGCGGATC CTACACTCAC CAAAGCCCCT
    CCCTCTTCCA TCGAGAGTGT GGTGCAGTCT GATACCCCCA CTCCGCCTCC TTCCCAGCCC
    TTAAATGAGA CAGCTGAAGA AGAAAGTCGG ATAAGCAGTT CTCCCACACT TCTGGAGAAC
    AGCTTGGAAC AGACCGTGGA GCCCAGCGAA GACCACAGTG AGGATCAGAG TGAGGGGGGC
    AGCGAAGACC TCGGTGAGCC TGTTTATGAA GAGCTAGCCA ATGAGCTAAG CGAGGAGCAG
    AGCAAAGCCG CCCTTCCCGG GGACCAGCAA GACCCCTCAT CCCTGTCCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa91871
    Clone ID Related Accession (Same CDS sequence) XM_015473451.1
    Accession Version XM_015473451.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2847bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product echinoderm microtubule-associated protein-like 4 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104293.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGAAGACA GCCTGCCTAG TCTCGCTTCA GTATGTGCTT CCTCTACACT TTCAAACTTC 
    TGTACTGATG ATAGTATTTC CGCTGCAAGT ACTTCTGATG TTCAGGATCG CCTCTCAGCC
    CTTGAGTTAC GAGTTCAACA GCAAGAAGAT GAAATCACAG TGCTAAAGGC AGCTTTGGCT
    GATGTTTTGA GGCGTCTTGC CATCTCTGAA GATCATGTAG CCTCGGTGAA AAAATCAGTC
    CCAAGTAAAG GCCAGCCAGG CCTTCGAGAA GCTATTTCTA TGTCCTGTAT AACCAACGGG
    AGTGGTACAA ACAGAAAACC AAGTCACACC AGCTCTGTCT CAATCGCAAG AAAAGAAACT
    CTTTCATCTG CTGCTAAAAG CATAAAACGA CCATCAACAG CTGAAAAATC ACATAATTCA
    TGGGAAAATT CAGACGATAG CCGTAATAAA TTATTGAAGG CAGTTTCAAC ATCAAAATTA
    ATATCAAAAG TTATAAAAAA TACAGACAAG CATAAAGATG TCATCATCAA CCAAGCAAAA
    ATGTCAACCC GTGAAAAAAA CAGCCAAGAA GGAGAATACA TTAAAATGTT TATGCGAGGT
    CGACCAATTA CAATGTTCAT TCCTTCTGAT GTTGAGAACT ATGATGACAT CAGAACAGAA
    CTGCCTCCTG AGAAGCTCAA ACTGGAGTGG GTATACGGTT ATCGAGGAAA GGATTGTCGA
    GCTAATGTTT ACCTTCTTCC TACCGGGGAA ATTGTTTATT TCATAGCATC CGTAGTAGTA
    CTGTTTAATT ATGAAGAGAG AACTCAACGG CACTACCTGG GTCATACAGA CTGTGTAAAA
    TGCCTTGCTA TACATCCTGA CAAAATTAGG ATTGCAACTG GACAGATAGC TGGAGTGGAT
    AAGGATGGAA GGCCCCTGCA ACCCCATGTC AGAGTGTGGG ATTCAGTTAG TCTTTCTACA
    CTGCAAATTA TTGGACTTGG AACTTTTGAA CGTGGAGTAG GATGCCTGGA TTTTTCAAAA
    GCAGATTCAG GTGTTCATTT ATGTGTTATC GATGACTCCA ATGAGCATAT GCTTACTGTG
    TGGGATTGGC AGAAAAAATC AAAAGGAGCA GAAATAAAGA CAACAAATGA AGTTGTTTTG
    GCTGTGGGGT TCCATCCAAC AGATGCAAAT ATCATAATAA CATGTGGTAA ATCACATATT
    TTTTTCTGGA CCTGGAGTGG CAATTCACTA ACAAAAAAAC AAGGAGTTTT TGGGAAATAC
    GAAAAACCAA AATTTGTGCA GTGTTTAGCT TTCTTGGGAA ATGGAGATGT TCTTGCTGGA
    GACTCAGGTG GAGTCATACT CATATGGAGC AAAACTACTG TAGAGCCCAC ACCTGGGAAA
    GGGCCTAAAG GTGTTTATCA GATCAGCAAA CAAATCAAAG CTCATGATGG CAGTGTGTTC
    ACACTTTGTC AGATGAGAAA TGGGATGTTA TTAACTGGAG GTGGGAAAGA CAGAAAAATA
    ATTCTGTGGG ATCATGATCT GAATCCTGAA AGAGAAATAG AGGTTCCTGA TCAGTATGGA
    ACAATCAGAG CTGTAGCAGA AGGAAAGGCA GATCAATTTT TGGTCGGCAC ATCACGAAAC
    TTTATTTTAC GAGGAACATT TAATGATGGC TTCCAAATAG AAGTACAGGG TCATACAGAT
    GAGCTTTGGG GTCTTGCCAC ACATCCCTTC AAAGACTTGC TCTTGACTTG TGCTCAGGAC
    AGACAGGTGT GCATGTGGAA CTCAGTAGAA CACAGACTGG AATGGACCAG GCTTCTAGAT
    GAACCAGGAC ACTGTGCAGA TTTTCATCCA AGTGGCACAG TGGTGGCCAT AGGAACGCAC
    TCAGGCAGGT GGTTTGTTCT GGATGCAGAA ACCAGAGATC TAGTTTCTAT CCACACAGAC
    GGAAATGAGC AGCTTTCGGT GATGCGCTAC TCAATAGATG GCACCCTCCT GGCTGTAGGA
    TCTCATGACA ACTTCATTTA CCTCTATGCC GTCTCAGAAA ATGGAAGAAA GTATAGCAGA
    CATGGAAAGT GCACTGGACA TTCCAGCTAT ATCACACACC TTGACTGGTC CCCAGACAAC
    AAGTATATAA TGTCTAACTC GGGAGACTAT GAGATCCTGT ACTGGGACAT TGAACATGGC
    TGCAAACTAA TGAGGAATCG ATCAGAATGT AAGGACATCA ATTGGACGAC ATACACCTGT
    GTGCTAGGCT TCCAAGTCTT TGGTGTCTGG CCAGAAGGAT CTGATGGGAC AGATATCAAC
    GCGCTGGTAC GATCCCACAA CAGAAAGGTG ATCGCTGTTG CTGATGACTT TTGCAAAGTC
    CATCTGTTTC AGTATCCCTG CTCCAAAGCA AAGGCTCCCA GTCACAAGTA CAGTGCCCAC
    AGCAGCCATG TCACCAATGT CAGTTTTACT CATAATGACA GTCACCTGAT TTCAACTGGT
    GGAAAAGACA TGAGCATCAT TCAGTGGAAA CTTGTGGAAA AGTTACCTTT GCCTCAAAAT
    GAGACTCTAG CGGATCCTAC ACTCACCAAA GCCCCTCCCT CTTCCATCGA GAGTGTGGTG
    CAGTCTGATA CCCCCACTCC GCCTCCTTCC CAGCCCTTAA ATGAGACAGC TGAAGAAGAA
    AGTCGGATAA GCAGTTCTCC CACACTTCTG GAGAACAGCT TGGAACAGAC CGTGGAGCCC
    AGCGAAGACC ACAGTGAGGA TCAGAGTGAG GGGGGCAGCG AAGACCTCGG TGAGCCTGTT
    TATGAAGAGC TAGCCAATGA GCTAAGCGAG GAGCAGAGCA AAGCCGCCCT TCCCGGGGAC
    CAGCAAGACC CCTCATCCCT GTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015328937.1
    CDS54..2900
    Translation

    Target ORF information:

    RefSeq Version XM_015473451.1
    Organism Bos taurus(cattle)
    Definition Bos taurus echinoderm microtubule associated protein like 4 (EML4), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015473451.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGAAGACA GCCTGCCTAG TCTCGCTTCA GTATGTGCTT CCTCTACACT TTCAAACTTC 
    TGTACTGATG ATAGTATTTC CGCTGCAAGT ACTTCTGATG TTCAGGATCG CCTCTCAGCC
    CTTGAGTTAC GAGTTCAACA GCAAGAAGAT GAAATCACAG TGCTAAAGGC AGCTTTGGCT
    GATGTTTTGA GGCGTCTTGC CATCTCTGAA GATCATGTAG CCTCGGTGAA AAAATCAGTC
    CCAAGTAAAG GCCAGCCAGG CCTTCGAGAA GCTATTTCTA TGTCCTGTAT AACCAACGGG
    AGTGGTACAA ACAGAAAACC AAGTCACACC AGCTCTGTCT CAATCGCAAG AAAAGAAACT
    CTTTCATCTG CTGCTAAAAG CATAAAACGA CCATCAACAG CTGAAAAATC ACATAATTCA
    TGGGAAAATT CAGACGATAG CCGTAATAAA TTATTGAAGG CAGTTTCAAC ATCAAAATTA
    ATATCAAAAG TTATAAAAAA TACAGACAAG CATAAAGATG TCATCATCAA CCAAGCAAAA
    ATGTCAACCC GTGAAAAAAA CAGCCAAGAA GGAGAATACA TTAAAATGTT TATGCGAGGT
    CGACCAATTA CAATGTTCAT TCCTTCTGAT GTTGAGAACT ATGATGACAT CAGAACAGAA
    CTGCCTCCTG AGAAGCTCAA ACTGGAGTGG GTATACGGTT ATCGAGGAAA GGATTGTCGA
    GCTAATGTTT ACCTTCTTCC TACCGGGGAA ATTGTTTATT TCATAGCATC CGTAGTAGTA
    CTGTTTAATT ATGAAGAGAG AACTCAACGG CACTACCTGG GTCATACAGA CTGTGTAAAA
    TGCCTTGCTA TACATCCTGA CAAAATTAGG ATTGCAACTG GACAGATAGC TGGAGTGGAT
    AAGGATGGAA GGCCCCTGCA ACCCCATGTC AGAGTGTGGG ATTCAGTTAG TCTTTCTACA
    CTGCAAATTA TTGGACTTGG AACTTTTGAA CGTGGAGTAG GATGCCTGGA TTTTTCAAAA
    GCAGATTCAG GTGTTCATTT ATGTGTTATC GATGACTCCA ATGAGCATAT GCTTACTGTG
    TGGGATTGGC AGAAAAAATC AAAAGGAGCA GAAATAAAGA CAACAAATGA AGTTGTTTTG
    GCTGTGGGGT TCCATCCAAC AGATGCAAAT ATCATAATAA CATGTGGTAA ATCACATATT
    TTTTTCTGGA CCTGGAGTGG CAATTCACTA ACAAAAAAAC AAGGAGTTTT TGGGAAATAC
    GAAAAACCAA AATTTGTGCA GTGTTTAGCT TTCTTGGGAA ATGGAGATGT TCTTGCTGGA
    GACTCAGGTG GAGTCATACT CATATGGAGC AAAACTACTG TAGAGCCCAC ACCTGGGAAA
    GGGCCTAAAG GTGTTTATCA GATCAGCAAA CAAATCAAAG CTCATGATGG CAGTGTGTTC
    ACACTTTGTC AGATGAGAAA TGGGATGTTA TTAACTGGAG GTGGGAAAGA CAGAAAAATA
    ATTCTGTGGG ATCATGATCT GAATCCTGAA AGAGAAATAG AGGTTCCTGA TCAGTATGGA
    ACAATCAGAG CTGTAGCAGA AGGAAAGGCA GATCAATTTT TGGTCGGCAC ATCACGAAAC
    TTTATTTTAC GAGGAACATT TAATGATGGC TTCCAAATAG AAGTACAGGG TCATACAGAT
    GAGCTTTGGG GTCTTGCCAC ACATCCCTTC AAAGACTTGC TCTTGACTTG TGCTCAGGAC
    AGACAGGTGT GCATGTGGAA CTCAGTAGAA CACAGACTGG AATGGACCAG GCTTCTAGAT
    GAACCAGGAC ACTGTGCAGA TTTTCATCCA AGTGGCACAG TGGTGGCCAT AGGAACGCAC
    TCAGGCAGGT GGTTTGTTCT GGATGCAGAA ACCAGAGATC TAGTTTCTAT CCACACAGAC
    GGAAATGAGC AGCTTTCGGT GATGCGCTAC TCAATAGATG GCACCCTCCT GGCTGTAGGA
    TCTCATGACA ACTTCATTTA CCTCTATGCC GTCTCAGAAA ATGGAAGAAA GTATAGCAGA
    CATGGAAAGT GCACTGGACA TTCCAGCTAT ATCACACACC TTGACTGGTC CCCAGACAAC
    AAGTATATAA TGTCTAACTC GGGAGACTAT GAGATCCTGT ACTGGGACAT TGAACATGGC
    TGCAAACTAA TGAGGAATCG ATCAGAATGT AAGGACATCA ATTGGACGAC ATACACCTGT
    GTGCTAGGCT TCCAAGTCTT TGGTGTCTGG CCAGAAGGAT CTGATGGGAC AGATATCAAC
    GCGCTGGTAC GATCCCACAA CAGAAAGGTG ATCGCTGTTG CTGATGACTT TTGCAAAGTC
    CATCTGTTTC AGTATCCCTG CTCCAAAGCA AAGGCTCCCA GTCACAAGTA CAGTGCCCAC
    AGCAGCCATG TCACCAATGT CAGTTTTACT CATAATGACA GTCACCTGAT TTCAACTGGT
    GGAAAAGACA TGAGCATCAT TCAGTGGAAA CTTGTGGAAA AGTTACCTTT GCCTCAAAAT
    GAGACTCTAG CGGATCCTAC ACTCACCAAA GCCCCTCCCT CTTCCATCGA GAGTGTGGTG
    CAGTCTGATA CCCCCACTCC GCCTCCTTCC CAGCCCTTAA ATGAGACAGC TGAAGAAGAA
    AGTCGGATAA GCAGTTCTCC CACACTTCTG GAGAACAGCT TGGAACAGAC CGTGGAGCCC
    AGCGAAGACC ACAGTGAGGA TCAGAGTGAG GGGGGCAGCG AAGACCTCGG TGAGCCTGTT
    TATGAAGAGC TAGCCAATGA GCTAAGCGAG GAGCAGAGCA AAGCCGCCCT TCCCGGGGAC
    CAGCAAGACC CCTCATCCCT GTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa91872
    Clone ID Related Accession (Same CDS sequence) XM_015473452.1
    Accession Version XM_015473452.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2814bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product echinoderm microtubule-associated protein-like 4 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104293.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGAAGACA GCCTGCCTAG TCTCGCTTCA GTATGTGCTT CCTCTACACT TTCAAACTTC 
    TGTACTGATG ATAGTATTTC CGCTGCAAGT ACTTCTGATG TTCAGGATCG CCTCTCAGCC
    CTTGAGTTAC GAGTTCAACA GCAAGAAGAT GAAATCACAG TGCTAAAGGC AGCTTTGGCT
    GATGTTTTGA GGCGTCTTGC CATCTCTGAA GATCATGTAG CCTCGGTGAA AAAATCAGTC
    CCAAGTAAAG GCCAGCCAGG CCTTCGAGAA GCTATTTCTA TGTCCTGTAT AACCAACGGG
    AGTGGTACAA ACAGAAAACC AAGTCACACC AGCTCTGTCT CAATCGCAAG AAAAGAAACT
    CTTTCATCTG CTGCTAAAAG CATAAAACGA CCATCAACAG CTGAAAAATC ACATAATTCA
    TGGGAAAATT CAGACGATAG CCGTAATAAA TTATTGAAGG CAGTTTCAAC ATCAAAATTA
    ATATCAAAAG TTATAAAAAA TACAGACAAG CATAAAGATG TCATCATCAA CCAAGAAGGA
    GAATACATTA AAATGTTTAT GCGAGGTCGA CCAATTACAA TGTTCATTCC TTCTGATGTT
    GAGAACTATG ATGACATCAG AACAGAACTG CCTCCTGAGA AGCTCAAACT GGAGTGGGTA
    TACGGTTATC GAGGAAAGGA TTGTCGAGCT AATGTTTACC TTCTTCCTAC CGGGGAAATT
    GTTTATTTCA TAGCATCCGT AGTAGTACTG TTTAATTATG AAGAGAGAAC TCAACGGCAC
    TACCTGGGTC ATACAGACTG TGTAAAATGC CTTGCTATAC ATCCTGACAA AATTAGGATT
    GCAACTGGAC AGATAGCTGG AGTGGATAAG GATGGAAGGC CCCTGCAACC CCATGTCAGA
    GTGTGGGATT CAGTTAGTCT TTCTACACTG CAAATTATTG GACTTGGAAC TTTTGAACGT
    GGAGTAGGAT GCCTGGATTT TTCAAAAGCA GATTCAGGTG TTCATTTATG TGTTATCGAT
    GACTCCAATG AGCATATGCT TACTGTGTGG GATTGGCAGA AAAAATCAAA AGGAGCAGAA
    ATAAAGACAA CAAATGAAGT TGTTTTGGCT GTGGGGTTCC ATCCAACAGA TGCAAATATC
    ATAATAACAT GTGGTAAATC ACATATTTTT TTCTGGACCT GGAGTGGCAA TTCACTAACA
    AAAAAACAAG GAGTTTTTGG GAAATACGAA AAACCAAAAT TTGTGCAGTG TTTAGCTTTC
    TTGGGAAATG GAGATGTTCT TGCTGGAGAC TCAGGTGGAG TCATACTCAT ATGGAGCAAA
    ACTACTGTAG AGCCCACACC TGGGAAAGGG CCTAAAGGTG TTTATCAGAT CAGCAAACAA
    ATCAAAGCTC ATGATGGCAG TGTGTTCACA CTTTGTCAGA TGAGAAATGG GATGTTATTA
    ACTGGAGGTG GGAAAGACAG AAAAATAATT CTGTGGGATC ATGATCTGAA TCCTGAAAGA
    GAAATAGAGG TTCCTGATCA GTATGGAACA ATCAGAGCTG TAGCAGAAGG AAAGGCAGAT
    CAATTTTTGG TCGGCACATC ACGAAACTTT ATTTTACGAG GAACATTTAA TGATGGCTTC
    CAAATAGAAG TACAGGGTCA TACAGATGAG CTTTGGGGTC TTGCCACACA TCCCTTCAAA
    GACTTGCTCT TGACTTGTGC TCAGGACAGA CAGGTGTGCA TGTGGAACTC AGTAGAACAC
    AGACTGGAAT GGACCAGGCT TCTAGATGAA CCAGGACACT GTGCAGATTT TCATCCAAGT
    GGCACAGTGG TGGCCATAGG AACGCACTCA GGCAGGTGGT TTGTTCTGGA TGCAGAAACC
    AGAGATCTAG TTTCTATCCA CACAGACGGA AATGAGCAGC TTTCGGTGAT GCGCTACTCA
    ATAGATGGCA CCCTCCTGGC TGTAGGATCT CATGACAACT TCATTTACCT CTATGCCGTC
    TCAGAAAATG GAAGAAAGTA TAGCAGACAT GGAAAGTGCA CTGGACATTC CAGCTATATC
    ACACACCTTG ACTGGTCCCC AGACAACAAG TATATAATGT CTAACTCGGG AGACTATGAG
    ATCCTGTACT GGGACATTGA ACATGGCTGC AAACTAATGA GGAATCGATC AGAATGTAAG
    GACATCAATT GGACGACATA CACCTGTGTG CTAGGCTTCC AAGTCTTTGG TGTCTGGCCA
    GAAGGATCTG ATGGGACAGA TATCAACGCG CTGGTACGAT CCCACAACAG AAAGGTGATC
    GCTGTTGCTG ATGACTTTTG CAAAGTCCAT CTGTTTCAGT ATCCCTGCTC CAAAGCAAAG
    GCTCCCAGTC ACAAGTACAG TGCCCACAGC AGCCATGTCA CCAATGTCAG TTTTACTCAT
    AATGACAGTC ACCTGATTTC AACTGGTGGA AAAGACATGA GCATCATTCA GTGGAAACTT
    GTGGAAAAGT TACCTTTGCC TCAAAATGAG ACTCTAGCGG ATCCTACACT CACCAAAGCC
    CCTCCCTCTT CCATCGAGAG TGTGGTGCAG TCTGATACCC CCACTCCGCC TCCTTCCCAG
    CCCTTAAATG AGACAGCTGA AGAAGAAAGT CGGATAAGCA GTTCTCCCAC ACTTCTGGAG
    AACAGCTTGG AACAGACCGT GGAGCCCAGC GAAGACCACA GTGAGGATCA GAGTGAGGGG
    GGCAGCGAAG ACCTCGGTGA GCCTGTTTAT GAAGAGCTAG CCAATGAGCT AAGCGAGGAG
    CAGAGCAAAG CCGCCCTTCC CGGGGACCAG CAAGACCCCT CATCCCTGTC CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015328938.1
    CDS54..2867
    Translation

    Target ORF information:

    RefSeq Version XM_015473452.1
    Organism Bos taurus(cattle)
    Definition Bos taurus echinoderm microtubule associated protein like 4 (EML4), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015473452.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGAAGACA GCCTGCCTAG TCTCGCTTCA GTATGTGCTT CCTCTACACT TTCAAACTTC 
    TGTACTGATG ATAGTATTTC CGCTGCAAGT ACTTCTGATG TTCAGGATCG CCTCTCAGCC
    CTTGAGTTAC GAGTTCAACA GCAAGAAGAT GAAATCACAG TGCTAAAGGC AGCTTTGGCT
    GATGTTTTGA GGCGTCTTGC CATCTCTGAA GATCATGTAG CCTCGGTGAA AAAATCAGTC
    CCAAGTAAAG GCCAGCCAGG CCTTCGAGAA GCTATTTCTA TGTCCTGTAT AACCAACGGG
    AGTGGTACAA ACAGAAAACC AAGTCACACC AGCTCTGTCT CAATCGCAAG AAAAGAAACT
    CTTTCATCTG CTGCTAAAAG CATAAAACGA CCATCAACAG CTGAAAAATC ACATAATTCA
    TGGGAAAATT CAGACGATAG CCGTAATAAA TTATTGAAGG CAGTTTCAAC ATCAAAATTA
    ATATCAAAAG TTATAAAAAA TACAGACAAG CATAAAGATG TCATCATCAA CCAAGAAGGA
    GAATACATTA AAATGTTTAT GCGAGGTCGA CCAATTACAA TGTTCATTCC TTCTGATGTT
    GAGAACTATG ATGACATCAG AACAGAACTG CCTCCTGAGA AGCTCAAACT GGAGTGGGTA
    TACGGTTATC GAGGAAAGGA TTGTCGAGCT AATGTTTACC TTCTTCCTAC CGGGGAAATT
    GTTTATTTCA TAGCATCCGT AGTAGTACTG TTTAATTATG AAGAGAGAAC TCAACGGCAC
    TACCTGGGTC ATACAGACTG TGTAAAATGC CTTGCTATAC ATCCTGACAA AATTAGGATT
    GCAACTGGAC AGATAGCTGG AGTGGATAAG GATGGAAGGC CCCTGCAACC CCATGTCAGA
    GTGTGGGATT CAGTTAGTCT TTCTACACTG CAAATTATTG GACTTGGAAC TTTTGAACGT
    GGAGTAGGAT GCCTGGATTT TTCAAAAGCA GATTCAGGTG TTCATTTATG TGTTATCGAT
    GACTCCAATG AGCATATGCT TACTGTGTGG GATTGGCAGA AAAAATCAAA AGGAGCAGAA
    ATAAAGACAA CAAATGAAGT TGTTTTGGCT GTGGGGTTCC ATCCAACAGA TGCAAATATC
    ATAATAACAT GTGGTAAATC ACATATTTTT TTCTGGACCT GGAGTGGCAA TTCACTAACA
    AAAAAACAAG GAGTTTTTGG GAAATACGAA AAACCAAAAT TTGTGCAGTG TTTAGCTTTC
    TTGGGAAATG GAGATGTTCT TGCTGGAGAC TCAGGTGGAG TCATACTCAT ATGGAGCAAA
    ACTACTGTAG AGCCCACACC TGGGAAAGGG CCTAAAGGTG TTTATCAGAT CAGCAAACAA
    ATCAAAGCTC ATGATGGCAG TGTGTTCACA CTTTGTCAGA TGAGAAATGG GATGTTATTA
    ACTGGAGGTG GGAAAGACAG AAAAATAATT CTGTGGGATC ATGATCTGAA TCCTGAAAGA
    GAAATAGAGG TTCCTGATCA GTATGGAACA ATCAGAGCTG TAGCAGAAGG AAAGGCAGAT
    CAATTTTTGG TCGGCACATC ACGAAACTTT ATTTTACGAG GAACATTTAA TGATGGCTTC
    CAAATAGAAG TACAGGGTCA TACAGATGAG CTTTGGGGTC TTGCCACACA TCCCTTCAAA
    GACTTGCTCT TGACTTGTGC TCAGGACAGA CAGGTGTGCA TGTGGAACTC AGTAGAACAC
    AGACTGGAAT GGACCAGGCT TCTAGATGAA CCAGGACACT GTGCAGATTT TCATCCAAGT
    GGCACAGTGG TGGCCATAGG AACGCACTCA GGCAGGTGGT TTGTTCTGGA TGCAGAAACC
    AGAGATCTAG TTTCTATCCA CACAGACGGA AATGAGCAGC TTTCGGTGAT GCGCTACTCA
    ATAGATGGCA CCCTCCTGGC TGTAGGATCT CATGACAACT TCATTTACCT CTATGCCGTC
    TCAGAAAATG GAAGAAAGTA TAGCAGACAT GGAAAGTGCA CTGGACATTC CAGCTATATC
    ACACACCTTG ACTGGTCCCC AGACAACAAG TATATAATGT CTAACTCGGG AGACTATGAG
    ATCCTGTACT GGGACATTGA ACATGGCTGC AAACTAATGA GGAATCGATC AGAATGTAAG
    GACATCAATT GGACGACATA CACCTGTGTG CTAGGCTTCC AAGTCTTTGG TGTCTGGCCA
    GAAGGATCTG ATGGGACAGA TATCAACGCG CTGGTACGAT CCCACAACAG AAAGGTGATC
    GCTGTTGCTG ATGACTTTTG CAAAGTCCAT CTGTTTCAGT ATCCCTGCTC CAAAGCAAAG
    GCTCCCAGTC ACAAGTACAG TGCCCACAGC AGCCATGTCA CCAATGTCAG TTTTACTCAT
    AATGACAGTC ACCTGATTTC AACTGGTGGA AAAGACATGA GCATCATTCA GTGGAAACTT
    GTGGAAAAGT TACCTTTGCC TCAAAATGAG ACTCTAGCGG ATCCTACACT CACCAAAGCC
    CCTCCCTCTT CCATCGAGAG TGTGGTGCAG TCTGATACCC CCACTCCGCC TCCTTCCCAG
    CCCTTAAATG AGACAGCTGA AGAAGAAAGT CGGATAAGCA GTTCTCCCAC ACTTCTGGAG
    AACAGCTTGG AACAGACCGT GGAGCCCAGC GAAGACCACA GTGAGGATCA GAGTGAGGGG
    GGCAGCGAAG ACCTCGGTGA GCCTGTTTAT GAAGAGCTAG CCAATGAGCT AAGCGAGGAG
    CAGAGCAAAG CCGCCCTTCC CGGGGACCAG CAAGACCCCT CATCCCTGTC CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa220578
    Clone ID Related Accession (Same CDS sequence) XM_005212656.2
    Accession Version XM_005212656.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3021bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product echinoderm microtubule-associated protein-like 4 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005212656.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGGAAGACA GCCTGCCTAG TCTCGCTTCA GTATGTGCTT CCTCTACACT CTCAAACTTC 
    TGTACTGATG ATAGTATTTC CGCTGCAAGT ACTTCTGATG TTCAGGATCG CCTCTCAGCC
    CTTGAGTTAC GAGTTCAACA GCAAGAAGAT GAAATCACAG TGCTAAAGGC AGCTTTGGCT
    GATGTTTTGA GGCGTCTTGC CATCTCTGAA GATCATGTAG CCTCGGTGAA AAAATCAGTC
    CCAAGTAAAG GCCAGCCAGG CCTTCGAGAA GCTATTTCTA TGTCCTGTAT AACCAACGGG
    AGTGGTACAA ACAGAAAACC AAGTCACACC AGCTCTGTCT CAATCGCAAG AAAAGAAACT
    CTTTCATCTG CTGCTAAAAG TGGTACAGAA AAAAAGAAAG AAAAACCACA AGGACAGAGA
    GAGAAAAAAG AGGACTCTCA CTCTAATGAT CAAAGTCCAC AAATTCGAGC ATCACCTTCT
    CCCCAGCCCT CTTCACAGCC TCTCCAAATA CACAGGCAAA CTCAAGAAAG CAAGAATTCT
    ACTCCCACCA AAAGCATAAA ACGACCATCA ACAGCTGAAA AATCACATAA TTCATGGGAA
    AATTCAGACG ATAGCCGTAA TAAATTATTG AAGGCAGTTT CAACATCAAA ATTAATATCA
    AAAGTTATAA AAAATACAGA CAAGCATAAA GATGTCATCA TCAACCAAGC AAAAATGTCA
    ACCCGTGAAA AAAACAGCCA AGAAGGAGAA TACATTAAAA TGTTTATGCG AGGTCGACCA
    ATTACAATGT TCATTCCTTC TGATGTTGAG AACTATGATG ACATCAGAAC AGAACTGCCT
    CCTGAGAAGC TCAAACTGGA GTGGGTATAC GGTTATCGAG GAAAGGATTG TCGAGCTAAT
    GTTTACCTTC TTCCTACCGG GGAAATTGTT TATTTCATAG CATCCGTAGT AGTACTGTTT
    AATTATGAAG AGAGAACTCA ACGGCACTAC CTGGGTCATA CAGACTGTGT AAAATGCCTT
    GCTATACATC CTGACAAAAT TAGGATTGCA ACTGGACAGA TAGCTGGAGT GGATAAGGAT
    GGAAGGCCCC TGCAACCCCA TGTCAGAGTG TGGGATTCAG TTAGTCTTTC TACACTGCAA
    ATTATTGGAC TTGGAACTTT TGAACGTGGA GTAGGATGCC TGGATTTTTC AAAAGCAGAT
    TCAGGTGTTC ATTTATGTGT TATCGATGAC TCCAATGAGC ATATGCTTAC TGTGTGGGAT
    TGGCAGAAAA AATCAAAAGG AGCAGAAATA AAGACAACAA ATGAAGTTGT TTTGGCTGTG
    GGGTTCCATC CAACAGATGC AAATATCATA ATAACATGTG GTAAATCACA TATTTTTTTC
    TGGACCTGGA GTGGCAATTC ACTAACAAAA AAACAAGGAG TTTTTGGGAA ATACGAAAAA
    CCAAAATTTG TGCAGTGTTT AGCTTTCTTG GGAAATGGAG ATGTTCTTGC TGGAGACTCA
    GGTGGAGTCA TACTCATATG GAGCAAAACT ACTGTAGAGC CCACACCTGG GAAAGGGCCT
    AAAGGTGTTT ATCAGATCAG CAAACAAATC AAAGCTCATG ATGGCAGTGT GTTCACACTT
    TGTCAGATGA GAAATGGGAT GTTATTAACT GGAGGTGGGA AAGACAGAAA AATAATTCTG
    TGGGATCATG ATCTGAATCC TGAAAGAGAA ATAGAGGTTC CTGATCAGTA TGGAACAATC
    AGAGCTGTAG CAGAAGGAAA GGCAGATCAA TTTTTGGTCG GCACATCACG AAACTTTATT
    TTACGAGGAA CATTTAATGA TGGCTTCCAA ATAGAAGTAC AGGGTCATAC AGATGAGCTT
    TGGGGTCTTG CCACACATCC CTTCAAAGAC TTGCTCTTGA CTTGTGCTCA GGACAGACAG
    GTGTGCATGT GGAACTCAGT AGAACACAGA CTGGAATGGA CCAGGCTTCT AGATGAACCA
    GGACACTGTG CAGATTTTCA TCCAAGTGGC ACAGTGGTGG CCATAGGAAC GCACTCAGGC
    AGGTGGTTTG TTCTGGATGC AGAAACCAGA GATCTAGTTT CTATCCACAC AGACGGAAAT
    GAGCAGCTTT CGGTGATGCG CTACTCAATA GATGGCACCC TCCTGGCTGT AGGATCTCAT
    GACAACTTCA TTTACCTCTA TGCCGTCTCA GAAAATGGAA GAAAGTATAG CAGACATGGA
    AAGTGCACTG GACATTCCAG CTATATCACA CACCTTGACT GGTCCCCAGA CAACAAGTAT
    ATAATGTCTA ACTCGGGAGA CTATGAGATC CTGTACTGGG ACATTGAACA TGGCTGCAAA
    CTAATGAGGA ATCGATCAGA ATGTAAGGAC ATCAATTGGA CGACATACAC CTGTGTGCTA
    GGCTTCCAAG TCTTTGGTGT CTGGCCAGAA GGATCTGATG GGACAGATAT CAACGCGCTG
    GTACGATCCC ACAACAGAAA GGTGATCGCT GTTGCTGATG ACTTTTGCAA AGTCCATCTG
    TTTCAGTATC CCTGCTCCAA AGCAAAGGCT CCCAGTCACA AGTACAGTGC CCACAGCAGC
    CATGTCACCA ATGTCAGTTT TACTCATAAT GACAGTCACC TGATTTCAAC TGGTGGAAAA
    GACATGAGCA TCATTCAGTG GAAACTTGTG GAAAAGTTAC CTTTGCCTCA AAATGAGACT
    CTAGCGGATC CTACACTCAC CAAAGCCCCT CCCTCTTCCA TCGAGAGTGT GGTGCAGTCT
    GATACCCCCA CTCCGCCTCC TTCCCAGCCC TTAAATGAGA CAGCTGAAGA AGAAAGTCGG
    ATAAGCAGTT CTCCCACACT TCTGGAGAAC AGCTTGGAAC AGACCGTGGA GCCCAGCGAA
    GACCACAGTG AGGATCAGAG TGAGGGGGGC AGCGAAGACC TCGGTGAGCC TGTTTATGAA
    GAGCTAGCCA ATGAGCTAAG CGAGGAGCAG AGCAAAGCCG CCCTTCCCGG GGACCAGCAA
    GACCCCTCAT CCCTGTCCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005212713.1
    CDS312..3332
    Translation

    Target ORF information:

    RefSeq Version XM_005212656.2
    Organism Bos taurus(cattle)
    Definition Bos taurus echinoderm microtubule associated protein like 4 (EML4), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005212656.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGGAAGACA GCCTGCCTAG TCTCGCTTCA GTATGTGCTT CCTCTACACT CTCAAACTTC 
    TGTACTGATG ATAGTATTTC CGCTGCAAGT ACTTCTGATG TTCAGGATCG CCTCTCAGCC
    CTTGAGTTAC GAGTTCAACA GCAAGAAGAT GAAATCACAG TGCTAAAGGC AGCTTTGGCT
    GATGTTTTGA GGCGTCTTGC CATCTCTGAA GATCATGTAG CCTCGGTGAA AAAATCAGTC
    CCAAGTAAAG GCCAGCCAGG CCTTCGAGAA GCTATTTCTA TGTCCTGTAT AACCAACGGG
    AGTGGTACAA ACAGAAAACC AAGTCACACC AGCTCTGTCT CAATCGCAAG AAAAGAAACT
    CTTTCATCTG CTGCTAAAAG TGGTACAGAA AAAAAGAAAG AAAAACCACA AGGACAGAGA
    GAGAAAAAAG AGGACTCTCA CTCTAATGAT CAAAGTCCAC AAATTCGAGC ATCACCTTCT
    CCCCAGCCCT CTTCACAGCC TCTCCAAATA CACAGGCAAA CTCAAGAAAG CAAGAATTCT
    ACTCCCACCA AAAGCATAAA ACGACCATCA ACAGCTGAAA AATCACATAA TTCATGGGAA
    AATTCAGACG ATAGCCGTAA TAAATTATTG AAGGCAGTTT CAACATCAAA ATTAATATCA
    AAAGTTATAA AAAATACAGA CAAGCATAAA GATGTCATCA TCAACCAAGC AAAAATGTCA
    ACCCGTGAAA AAAACAGCCA AGAAGGAGAA TACATTAAAA TGTTTATGCG AGGTCGACCA
    ATTACAATGT TCATTCCTTC TGATGTTGAG AACTATGATG ACATCAGAAC AGAACTGCCT
    CCTGAGAAGC TCAAACTGGA GTGGGTATAC GGTTATCGAG GAAAGGATTG TCGAGCTAAT
    GTTTACCTTC TTCCTACCGG GGAAATTGTT TATTTCATAG CATCCGTAGT AGTACTGTTT
    AATTATGAAG AGAGAACTCA ACGGCACTAC CTGGGTCATA CAGACTGTGT AAAATGCCTT
    GCTATACATC CTGACAAAAT TAGGATTGCA ACTGGACAGA TAGCTGGAGT GGATAAGGAT
    GGAAGGCCCC TGCAACCCCA TGTCAGAGTG TGGGATTCAG TTAGTCTTTC TACACTGCAA
    ATTATTGGAC TTGGAACTTT TGAACGTGGA GTAGGATGCC TGGATTTTTC AAAAGCAGAT
    TCAGGTGTTC ATTTATGTGT TATCGATGAC TCCAATGAGC ATATGCTTAC TGTGTGGGAT
    TGGCAGAAAA AATCAAAAGG AGCAGAAATA AAGACAACAA ATGAAGTTGT TTTGGCTGTG
    GGGTTCCATC CAACAGATGC AAATATCATA ATAACATGTG GTAAATCACA TATTTTTTTC
    TGGACCTGGA GTGGCAATTC ACTAACAAAA AAACAAGGAG TTTTTGGGAA ATACGAAAAA
    CCAAAATTTG TGCAGTGTTT AGCTTTCTTG GGAAATGGAG ATGTTCTTGC TGGAGACTCA
    GGTGGAGTCA TACTCATATG GAGCAAAACT ACTGTAGAGC CCACACCTGG GAAAGGGCCT
    AAAGGTGTTT ATCAGATCAG CAAACAAATC AAAGCTCATG ATGGCAGTGT GTTCACACTT
    TGTCAGATGA GAAATGGGAT GTTATTAACT GGAGGTGGGA AAGACAGAAA AATAATTCTG
    TGGGATCATG ATCTGAATCC TGAAAGAGAA ATAGAGGTTC CTGATCAGTA TGGAACAATC
    AGAGCTGTAG CAGAAGGAAA GGCAGATCAA TTTTTGGTCG GCACATCACG AAACTTTATT
    TTACGAGGAA CATTTAATGA TGGCTTCCAA ATAGAAGTAC AGGGTCATAC AGATGAGCTT
    TGGGGTCTTG CCACACATCC CTTCAAAGAC TTGCTCTTGA CTTGTGCTCA GGACAGACAG
    GTGTGCATGT GGAACTCAGT AGAACACAGA CTGGAATGGA CCAGGCTTCT AGATGAACCA
    GGACACTGTG CAGATTTTCA TCCAAGTGGC ACAGTGGTGG CCATAGGAAC GCACTCAGGC
    AGGTGGTTTG TTCTGGATGC AGAAACCAGA GATCTAGTTT CTATCCACAC AGACGGAAAT
    GAGCAGCTTT CGGTGATGCG CTACTCAATA GATGGCACCC TCCTGGCTGT AGGATCTCAT
    GACAACTTCA TTTACCTCTA TGCCGTCTCA GAAAATGGAA GAAAGTATAG CAGACATGGA
    AAGTGCACTG GACATTCCAG CTATATCACA CACCTTGACT GGTCCCCAGA CAACAAGTAT
    ATAATGTCTA ACTCGGGAGA CTATGAGATC CTGTACTGGG ACATTGAACA TGGCTGCAAA
    CTAATGAGGA ATCGATCAGA ATGTAAGGAC ATCAATTGGA CGACATACAC CTGTGTGCTA
    GGCTTCCAAG TCTTTGGTGT CTGGCCAGAA GGATCTGATG GGACAGATAT CAACGCGCTG
    GTACGATCCC ACAACAGAAA GGTGATCGCT GTTGCTGATG ACTTTTGCAA AGTCCATCTG
    TTTCAGTATC CCTGCTCCAA AGCAAAGGCT CCCAGTCACA AGTACAGTGC CCACAGCAGC
    CATGTCACCA ATGTCAGTTT TACTCATAAT GACAGTCACC TGATTTCAAC TGGTGGAAAA
    GACATGAGCA TCATTCAGTG GAAACTTGTG GAAAAGTTAC CTTTGCCTCA AAATGAGACT
    CTAGCGGATC CTACACTCAC CAAAGCCCCT CCCTCTTCCA TCGAGAGTGT GGTGCAGTCT
    GATACCCCCA CTCCGCCTCC TTCCCAGCCC TTAAATGAGA CAGCTGAAGA AGAAAGTCGG
    ATAAGCAGTT CTCCCACACT TCTGGAGAAC AGCTTGGAAC AGACCGTGGA GCCCAGCGAA
    GACCACAGTG AGGATCAGAG TGAGGGGGGC AGCGAAGACC TCGGTGAGCC TGTTTATGAA
    GAGCTAGCCA ATGAGCTAAG CGAGGAGCAG AGCAAAGCCG CCCTTCCCGG GGACCAGCAA
    GACCCCTCAT CCCTGTCCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa54581
    Clone ID Related Accession (Same CDS sequence) XM_024999050.1
    Accession Version XM_024999050.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3030bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product echinoderm microtubule-associated protein-like 4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGCTATGGA GCTCTCCAGA CAGCTGTCTG TGGGTGGTGG TGAGAGTGGA GGCGAACAGT 
    GGGTTGTGTG ACCAGGATGA TAGTATTTCC GCTGCAAGTA CTTCTGATGT TCAGGATCGC
    CTCTCAGCCC TTGAGTTACG AGTTCAACAG CAAGAAGATG AAATCACAGT GCTAAAGGCA
    GCTTTGGCTG ATGTTTTGAG GCGTCTTGCC ATCTCTGAAG ATCATGTAGC CTCGGTGAAA
    AAATCAGTCC CAAGTAAAGG CCAGCCAGGC CTTCGAGAAG CTATTTCTAT GTCCTGTATA
    ACCAACGGGA GTGGTACAAA CAGAAAACCA AGTCACACCA GCTCTGTCTC AATCGCAAGA
    AAAGAAACTC TTTCATCTGC TGCTAAAAGT GGTACAGAAA AAAAGAAAGA AAAACCACAA
    GGACAGAGAG AGAAAAAAGA GGACTCTCAC TCTAATGATC AAAGTCCACA AATTCGAGCA
    TCACCTTCTC CCCAGCCCTC TTCACAGCCT CTCCAAATAC ACAGGCAAAC TCAAGAAAGC
    AAGAATTCTA CTCCCACCAA AAGCATAAAA CGACCATCAA CAGCTGAAAA ATCACATAAT
    TCATGGGAAA ATTCAGACGA TAGCCGTAAT AAATTATTGA AGGCAGTTTC AACATCAAAA
    TTAATATCAA AAGTTATAAA AAATACAGAC AAGCATAAAG ATGTCATCAT CAACCAAGCA
    AAAATGTCAA CCCGTGAAAA AAACAGCCAA GAAGGAGAAT ACATTAAAAT GTTTATGCGA
    GGTCGACCAA TTACAATGTT CATTCCTTCT GATGTTGAGA ACTATGATGA CATCAGAACA
    GAACTGCCTC CTGAGAAGCT CAAACTGGAG TGGGTATACG GTTATCGAGG AAAGGATTGT
    CGAGCTAATG TTTACCTTCT TCCTACCGGG GAAATTGTTT ATTTCATAGC ATCCGTAGTA
    GTACTGTTTA ATTATGAAGA GAGAACTCAA CGGCACTACC TGGGTCATAC AGACTGTGTA
    AAATGCCTTG CTATACATCC TGACAAAATT AGGATTGCAA CTGGACAGAT AGCTGGAGTG
    GATAAGGATG GAAGGCCCCT GCAACCCCAT GTCAGAGTGT GGGATTCAGT TAGTCTTTCT
    ACACTGCAAA TTATTGGACT TGGAACTTTT GAACGTGGAG TAGGATGCCT GGATTTTTCA
    AAAGCAGATT CAGGTGTTCA TTTATGTGTT ATCGATGACT CCAATGAGCA TATGCTTACT
    GTGTGGGATT GGCAGAAAAA ATCAAAAGGA GCAGAAATAA AGACAACAAA TGAAGTTGTT
    TTGGCTGTGG GGTTCCATCC AACAGATGCA AATATCATAA TAACATGTGG TAAATCACAT
    ATTTTTTTCT GGACCTGGAG TGGCAATTCA CTAACAAAAA AACAAGGAGT TTTTGGGAAA
    TACGAAAAAC CAAAATTTGT GCAGTGTTTA GCTTTCTTGG GAAATGGAGA TGTTCTTGCT
    GGAGACTCAG GTGGAGTCAT ACTCATATGG AGCAAAACTA CTGTAGAGCC CACACCTGGG
    AAAGGGCCTA AAGGTGTTTA TCAGATCAGC AAACAAATCA AAGCTCATGA TGGCAGTGTG
    TTCACACTTT GTCAGATGAG AAATGGGATG TTATTAACTG GAGGTGGGAA AGACAGAAAA
    ATAATTCTGT GGGATCATGA TCTGAATCCT GAAAGAGAAA TAGAGGTTCC TGATCAGTAT
    GGAACAATCA GAGCTGTAGC AGAAGGAAAG GCAGATCAAT TTTTGGTCGG CACATCACGA
    AACTTTATTT TACGAGGAAC ATTTAATGAT GGCTTCCAAA TAGAAGTACA GGGTCATACA
    GATGAGCTTT GGGGTCTTGC CACACATCCC TTCAAAGACT TGCTCTTGAC TTGTGCTCAG
    GACAGACAGG TGTGCATGTG GAACTCAGTA GAACACAGAC TGGAATGGAC CAGGCTTCTA
    GATGAACCAG GACACTGTGC AGATTTTCAT CCAAGTGGCA CAGTGGTGGC CATAGGAACG
    CACTCAGGCA GGTGGTTTGT TCTGGATGCA GAAACCAGAG ATCTAGTTTC TATCCACACA
    GACGGAAATG AGCAGCTTTC GGTGATGCGC TACTCAATAG ATGGCACCCT CCTGGCTGTA
    GGATCTCATG ACAACTTCAT TTACCTCTAT GCCGTCTCAG AAAATGGAAG AAAGTATAGC
    AGACATGGAA AGTGCACTGG ACATTCCAGC TATATCACAC ACCTTGACTG GTCCCCAGAC
    AACAAGTATA TAATGTCTAA CTCGGGAGAC TATGAGATCC TGTACTGGGA CATTGAACAT
    GGCTGCAAAC TAATGAGGAA TCGATCAGAA TGTAAGGACA TCAATTGGAC GACATACACC
    TGTGTGCTAG GCTTCCAAGT CTTTGGTGTC TGGCCAGAAG GATCTGATGG GACAGATATC
    AACGCGCTGG TACGATCCCA CAACAGAAAG GTGATCGCTG TTGCTGATGA CTTTTGCAAA
    GTCCATCTGT TTCAGTATCC CTGCTCCAAA GCAAAGGCTC CCAGTCACAA GTACAGTGCC
    CACAGCAGCC ATGTCACCAA TGTCAGTTTT ACTCATAATG ACAGTCACCT GATTTCAACT
    GGTGGAAAAG ACATGAGCAT CATTCAGTGG AAACTTGTGG AAAAGTTACC TTTGCCTCAA
    AATGAGACTC TAGCGGATCC TACACTCACC AAAGCCCCTC CCTCTTCCAT CGAGAGTGTG
    GTGCAGTCTG ATACCCCCAC TCCGCCTCCT TCCCAGCCCT TAAATGAGAC AGCTGAAGAA
    GAAAGTCGGA TAAGCAGTTC TCCCACACTT CTGGAGAACA GCTTGGAACA GACCGTGGAG
    CCCAGCGAAG ACCACAGTGA GGATCAGAGT GAGGGGGGCA GCGAAGACCT CGGTGAGCCT
    GTTTATGAAG AGCTAGCCAA TGAGCTAAGC GAGGAGCAGA GCAAAGCCGC CCTTCCCGGG
    GACCAGCAAG ACCCCTCATC CCTGTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024854818.1
    CDS16..3045
    Translation

    Target ORF information:

    RefSeq Version XM_024999050.1
    Organism Bos taurus(cattle)
    Definition Bos taurus echinoderm microtubule associated protein like 4 (EML4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024999050.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGCTATGGA GCTCTCCAGA CAGCTGTCTG TGGGTGGTGG TGAGAGTGGA GGCGAACAGT 
    GGGTTGTGTG ACCAGGATGA TAGTATTTCC GCTGCAAGTA CTTCTGATGT TCAGGATCGC
    CTCTCAGCCC TTGAGTTACG AGTTCAACAG CAAGAAGATG AAATCACAGT GCTAAAGGCA
    GCTTTGGCTG ATGTTTTGAG GCGTCTTGCC ATCTCTGAAG ATCATGTAGC CTCGGTGAAA
    AAATCAGTCC CAAGTAAAGG CCAGCCAGGC CTTCGAGAAG CTATTTCTAT GTCCTGTATA
    ACCAACGGGA GTGGTACAAA CAGAAAACCA AGTCACACCA GCTCTGTCTC AATCGCAAGA
    AAAGAAACTC TTTCATCTGC TGCTAAAAGT GGTACAGAAA AAAAGAAAGA AAAACCACAA
    GGACAGAGAG AGAAAAAAGA GGACTCTCAC TCTAATGATC AAAGTCCACA AATTCGAGCA
    TCACCTTCTC CCCAGCCCTC TTCACAGCCT CTCCAAATAC ACAGGCAAAC TCAAGAAAGC
    AAGAATTCTA CTCCCACCAA AAGCATAAAA CGACCATCAA CAGCTGAAAA ATCACATAAT
    TCATGGGAAA ATTCAGACGA TAGCCGTAAT AAATTATTGA AGGCAGTTTC AACATCAAAA
    TTAATATCAA AAGTTATAAA AAATACAGAC AAGCATAAAG ATGTCATCAT CAACCAAGCA
    AAAATGTCAA CCCGTGAAAA AAACAGCCAA GAAGGAGAAT ACATTAAAAT GTTTATGCGA
    GGTCGACCAA TTACAATGTT CATTCCTTCT GATGTTGAGA ACTATGATGA CATCAGAACA
    GAACTGCCTC CTGAGAAGCT CAAACTGGAG TGGGTATACG GTTATCGAGG AAAGGATTGT
    CGAGCTAATG TTTACCTTCT TCCTACCGGG GAAATTGTTT ATTTCATAGC ATCCGTAGTA
    GTACTGTTTA ATTATGAAGA GAGAACTCAA CGGCACTACC TGGGTCATAC AGACTGTGTA
    AAATGCCTTG CTATACATCC TGACAAAATT AGGATTGCAA CTGGACAGAT AGCTGGAGTG
    GATAAGGATG GAAGGCCCCT GCAACCCCAT GTCAGAGTGT GGGATTCAGT TAGTCTTTCT
    ACACTGCAAA TTATTGGACT TGGAACTTTT GAACGTGGAG TAGGATGCCT GGATTTTTCA
    AAAGCAGATT CAGGTGTTCA TTTATGTGTT ATCGATGACT CCAATGAGCA TATGCTTACT
    GTGTGGGATT GGCAGAAAAA ATCAAAAGGA GCAGAAATAA AGACAACAAA TGAAGTTGTT
    TTGGCTGTGG GGTTCCATCC AACAGATGCA AATATCATAA TAACATGTGG TAAATCACAT
    ATTTTTTTCT GGACCTGGAG TGGCAATTCA CTAACAAAAA AACAAGGAGT TTTTGGGAAA
    TACGAAAAAC CAAAATTTGT GCAGTGTTTA GCTTTCTTGG GAAATGGAGA TGTTCTTGCT
    GGAGACTCAG GTGGAGTCAT ACTCATATGG AGCAAAACTA CTGTAGAGCC CACACCTGGG
    AAAGGGCCTA AAGGTGTTTA TCAGATCAGC AAACAAATCA AAGCTCATGA TGGCAGTGTG
    TTCACACTTT GTCAGATGAG AAATGGGATG TTATTAACTG GAGGTGGGAA AGACAGAAAA
    ATAATTCTGT GGGATCATGA TCTGAATCCT GAAAGAGAAA TAGAGGTTCC TGATCAGTAT
    GGAACAATCA GAGCTGTAGC AGAAGGAAAG GCAGATCAAT TTTTGGTCGG CACATCACGA
    AACTTTATTT TACGAGGAAC ATTTAATGAT GGCTTCCAAA TAGAAGTACA GGGTCATACA
    GATGAGCTTT GGGGTCTTGC CACACATCCC TTCAAAGACT TGCTCTTGAC TTGTGCTCAG
    GACAGACAGG TGTGCATGTG GAACTCAGTA GAACACAGAC TGGAATGGAC CAGGCTTCTA
    GATGAACCAG GACACTGTGC AGATTTTCAT CCAAGTGGCA CAGTGGTGGC CATAGGAACG
    CACTCAGGCA GGTGGTTTGT TCTGGATGCA GAAACCAGAG ATCTAGTTTC TATCCACACA
    GACGGAAATG AGCAGCTTTC GGTGATGCGC TACTCAATAG ATGGCACCCT CCTGGCTGTA
    GGATCTCATG ACAACTTCAT TTACCTCTAT GCCGTCTCAG AAAATGGAAG AAAGTATAGC
    AGACATGGAA AGTGCACTGG ACATTCCAGC TATATCACAC ACCTTGACTG GTCCCCAGAC
    AACAAGTATA TAATGTCTAA CTCGGGAGAC TATGAGATCC TGTACTGGGA CATTGAACAT
    GGCTGCAAAC TAATGAGGAA TCGATCAGAA TGTAAGGACA TCAATTGGAC GACATACACC
    TGTGTGCTAG GCTTCCAAGT CTTTGGTGTC TGGCCAGAAG GATCTGATGG GACAGATATC
    AACGCGCTGG TACGATCCCA CAACAGAAAG GTGATCGCTG TTGCTGATGA CTTTTGCAAA
    GTCCATCTGT TTCAGTATCC CTGCTCCAAA GCAAAGGCTC CCAGTCACAA GTACAGTGCC
    CACAGCAGCC ATGTCACCAA TGTCAGTTTT ACTCATAATG ACAGTCACCT GATTTCAACT
    GGTGGAAAAG ACATGAGCAT CATTCAGTGG AAACTTGTGG AAAAGTTACC TTTGCCTCAA
    AATGAGACTC TAGCGGATCC TACACTCACC AAAGCCCCTC CCTCTTCCAT CGAGAGTGTG
    GTGCAGTCTG ATACCCCCAC TCCGCCTCCT TCCCAGCCCT TAAATGAGAC AGCTGAAGAA
    GAAAGTCGGA TAAGCAGTTC TCCCACACTT CTGGAGAACA GCTTGGAACA GACCGTGGAG
    CCCAGCGAAG ACCACAGTGA GGATCAGAGT GAGGGGGGCA GCGAAGACCT CGGTGAGCCT
    GTTTATGAAG AGCTAGCCAA TGAGCTAAGC GAGGAGCAGA GCAAAGCCGC CCTTCCCGGG
    GACCAGCAAG ACCCCTCATC CCTGTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa91868
    Clone ID Related Accession (Same CDS sequence) XM_015473448.1
    Accession Version XM_015473448.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3045bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product echinoderm microtubule-associated protein-like 4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104293.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGGAAGACA GCCTGCCTAG TCTCGCTTCA GTATGTGCTT CCTCTACACT TTCAAACTTC 
    TGTACTGATG ATAGTATTTC CGCTGCAAGT ACTTCTGATG TTCAGGATCG CCTCTCAGCC
    CTTGAGTTAC GAGTTCAACA GCAAGAAGAT GAAATCACAG TGCTAAAGGC AGCTTTGGCT
    GATGTTTTGA GGCGTCTTGC CATCTCTGAA GATCATGTAG CCTCGGTGAA AAAATCAGTC
    CCAAGTAAAG GCCAGCCAGG CCTTCGAGAA GCTATTTCTA TGTCCTGTAT AACCAACGGG
    AGTGGTACAA ACAGAAAACC AAGTCACACC AGCTCTGTCT CAATCGCAAG AAAAGAAACT
    CTTTCATCTG CTGCTAAAAG TGGTACAGAA AAAAAGAAAG AAAAACCACA AGGACAGAGA
    GAGAAAAAAG AGGACTCTCA CTCTAATGAT CAAAGTCCAC AAATTCGAGC ATCACCTTCT
    CCCCAGCCCT CTTCACAGCC TCTCCAAATA CACAGGCAAA CTCAAGAAAG CAAGAATTCT
    ACTCCCACCA AAAGCATAAA ACGACCATCA ACAGCTGAAA AATCACATAA TTCATGGGAA
    AATTCAGACG ATAGCCGTAA TAAATTATTG AAGGCAGTTT CAACATCAAA ATTAATATCA
    AAAGTTATAA AAAATACAGA CAAGCATAAA GATGTCATCA TCAACCAAGA TATGTCATTT
    TTGTCTCAAC CAGCAAAAAT GTCAACCCGT GAAAAAAACA GCCAAGAAGG AGAATACATT
    AAAATGTTTA TGCGAGGTCG ACCAATTACA ATGTTCATTC CTTCTGATGT TGAGAACTAT
    GATGACATCA GAACAGAACT GCCTCCTGAG AAGCTCAAAC TGGAGTGGGT ATACGGTTAT
    CGAGGAAAGG ATTGTCGAGC TAATGTTTAC CTTCTTCCTA CCGGGGAAAT TGTTTATTTC
    ATAGCATCCG TAGTAGTACT GTTTAATTAT GAAGAGAGAA CTCAACGGCA CTACCTGGGT
    CATACAGACT GTGTAAAATG CCTTGCTATA CATCCTGACA AAATTAGGAT TGCAACTGGA
    CAGATAGCTG GAGTGGATAA GGATGGAAGG CCCCTGCAAC CCCATGTCAG AGTGTGGGAT
    TCAGTTAGTC TTTCTACACT GCAAATTATT GGACTTGGAA CTTTTGAACG TGGAGTAGGA
    TGCCTGGATT TTTCAAAAGC AGATTCAGGT GTTCATTTAT GTGTTATCGA TGACTCCAAT
    GAGCATATGC TTACTGTGTG GGATTGGCAG AAAAAATCAA AAGGAGCAGA AATAAAGACA
    ACAAATGAAG TTGTTTTGGC TGTGGGGTTC CATCCAACAG ATGCAAATAT CATAATAACA
    TGTGGTAAAT CACATATTTT TTTCTGGACC TGGAGTGGCA ATTCACTAAC AAAAAAACAA
    GGAGTTTTTG GGAAATACGA AAAACCAAAA TTTGTGCAGT GTTTAGCTTT CTTGGGAAAT
    GGAGATGTTC TTGCTGGAGA CTCAGGTGGA GTCATACTCA TATGGAGCAA AACTACTGTA
    GAGCCCACAC CTGGGAAAGG GCCTAAAGGT GTTTATCAGA TCAGCAAACA AATCAAAGCT
    CATGATGGCA GTGTGTTCAC ACTTTGTCAG ATGAGAAATG GGATGTTATT AACTGGAGGT
    GGGAAAGACA GAAAAATAAT TCTGTGGGAT CATGATCTGA ATCCTGAAAG AGAAATAGAG
    GTTCCTGATC AGTATGGAAC AATCAGAGCT GTAGCAGAAG GAAAGGCAGA TCAATTTTTG
    GTCGGCACAT CACGAAACTT TATTTTACGA GGAACATTTA ATGATGGCTT CCAAATAGAA
    GTACAGGGTC ATACAGATGA GCTTTGGGGT CTTGCCACAC ATCCCTTCAA AGACTTGCTC
    TTGACTTGTG CTCAGGACAG ACAGGTGTGC ATGTGGAACT CAGTAGAACA CAGACTGGAA
    TGGACCAGGC TTCTAGATGA ACCAGGACAC TGTGCAGATT TTCATCCAAG TGGCACAGTG
    GTGGCCATAG GAACGCACTC AGGCAGGTGG TTTGTTCTGG ATGCAGAAAC CAGAGATCTA
    GTTTCTATCC ACACAGACGG AAATGAGCAG CTTTCGGTGA TGCGCTACTC AATAGATGGC
    ACCCTCCTGG CTGTAGGATC TCATGACAAC TTCATTTACC TCTATGCCGT CTCAGAAAAT
    GGAAGAAAGT ATAGCAGACA TGGAAAGTGC ACTGGACATT CCAGCTATAT CACACACCTT
    GACTGGTCCC CAGACAACAA GTATATAATG TCTAACTCGG GAGACTATGA GATCCTGTAC
    TGGGACATTG AACATGGCTG CAAACTAATG AGGAATCGAT CAGAATGTAA GGACATCAAT
    TGGACGACAT ACACCTGTGT GCTAGGCTTC CAAGTCTTTG GTGTCTGGCC AGAAGGATCT
    GATGGGACAG ATATCAACGC GCTGGTACGA TCCCACAACA GAAAGGTGAT CGCTGTTGCT
    GATGACTTTT GCAAAGTCCA TCTGTTTCAG TATCCCTGCT CCAAAGCAAA GGCTCCCAGT
    CACAAGTACA GTGCCCACAG CAGCCATGTC ACCAATGTCA GTTTTACTCA TAATGACAGT
    CACCTGATTT CAACTGGTGG AAAAGACATG AGCATCATTC AGTGGAAACT TGTGGAAAAG
    TTACCTTTGC CTCAAAATGA GACTCTAGCG GATCCTACAC TCACCAAAGC CCCTCCCTCT
    TCCATCGAGA GTGTGGTGCA GTCTGATACC CCCACTCCGC CTCCTTCCCA GCCCTTAAAT
    GAGACAGCTG AAGAAGAAAG TCGGATAAGC AGTTCTCCCA CACTTCTGGA GAACAGCTTG
    GAACAGACCG TGGAGCCCAG CGAAGACCAC AGTGAGGATC AGAGTGAGGG GGGCAGCGAA
    GACCTCGGTG AGCCTGTTTA TGAAGAGCTA GCCAATGAGC TAAGCGAGGA GCAGAGCAAA
    GCCGCCCTTC CCGGGGACCA GCAAGACCCC TCATCCCTGT CCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015328934.1
    CDS307..3351
    Translation

    Target ORF information:

    RefSeq Version XM_015473448.1
    Organism Bos taurus(cattle)
    Definition Bos taurus echinoderm microtubule associated protein like 4 (EML4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015473448.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGGAAGACA GCCTGCCTAG TCTCGCTTCA GTATGTGCTT CCTCTACACT TTCAAACTTC 
    TGTACTGATG ATAGTATTTC CGCTGCAAGT ACTTCTGATG TTCAGGATCG CCTCTCAGCC
    CTTGAGTTAC GAGTTCAACA GCAAGAAGAT GAAATCACAG TGCTAAAGGC AGCTTTGGCT
    GATGTTTTGA GGCGTCTTGC CATCTCTGAA GATCATGTAG CCTCGGTGAA AAAATCAGTC
    CCAAGTAAAG GCCAGCCAGG CCTTCGAGAA GCTATTTCTA TGTCCTGTAT AACCAACGGG
    AGTGGTACAA ACAGAAAACC AAGTCACACC AGCTCTGTCT CAATCGCAAG AAAAGAAACT
    CTTTCATCTG CTGCTAAAAG TGGTACAGAA AAAAAGAAAG AAAAACCACA AGGACAGAGA
    GAGAAAAAAG AGGACTCTCA CTCTAATGAT CAAAGTCCAC AAATTCGAGC ATCACCTTCT
    CCCCAGCCCT CTTCACAGCC TCTCCAAATA CACAGGCAAA CTCAAGAAAG CAAGAATTCT
    ACTCCCACCA AAAGCATAAA ACGACCATCA ACAGCTGAAA AATCACATAA TTCATGGGAA
    AATTCAGACG ATAGCCGTAA TAAATTATTG AAGGCAGTTT CAACATCAAA ATTAATATCA
    AAAGTTATAA AAAATACAGA CAAGCATAAA GATGTCATCA TCAACCAAGA TATGTCATTT
    TTGTCTCAAC CAGCAAAAAT GTCAACCCGT GAAAAAAACA GCCAAGAAGG AGAATACATT
    AAAATGTTTA TGCGAGGTCG ACCAATTACA ATGTTCATTC CTTCTGATGT TGAGAACTAT
    GATGACATCA GAACAGAACT GCCTCCTGAG AAGCTCAAAC TGGAGTGGGT ATACGGTTAT
    CGAGGAAAGG ATTGTCGAGC TAATGTTTAC CTTCTTCCTA CCGGGGAAAT TGTTTATTTC
    ATAGCATCCG TAGTAGTACT GTTTAATTAT GAAGAGAGAA CTCAACGGCA CTACCTGGGT
    CATACAGACT GTGTAAAATG CCTTGCTATA CATCCTGACA AAATTAGGAT TGCAACTGGA
    CAGATAGCTG GAGTGGATAA GGATGGAAGG CCCCTGCAAC CCCATGTCAG AGTGTGGGAT
    TCAGTTAGTC TTTCTACACT GCAAATTATT GGACTTGGAA CTTTTGAACG TGGAGTAGGA
    TGCCTGGATT TTTCAAAAGC AGATTCAGGT GTTCATTTAT GTGTTATCGA TGACTCCAAT
    GAGCATATGC TTACTGTGTG GGATTGGCAG AAAAAATCAA AAGGAGCAGA AATAAAGACA
    ACAAATGAAG TTGTTTTGGC TGTGGGGTTC CATCCAACAG ATGCAAATAT CATAATAACA
    TGTGGTAAAT CACATATTTT TTTCTGGACC TGGAGTGGCA ATTCACTAAC AAAAAAACAA
    GGAGTTTTTG GGAAATACGA AAAACCAAAA TTTGTGCAGT GTTTAGCTTT CTTGGGAAAT
    GGAGATGTTC TTGCTGGAGA CTCAGGTGGA GTCATACTCA TATGGAGCAA AACTACTGTA
    GAGCCCACAC CTGGGAAAGG GCCTAAAGGT GTTTATCAGA TCAGCAAACA AATCAAAGCT
    CATGATGGCA GTGTGTTCAC ACTTTGTCAG ATGAGAAATG GGATGTTATT AACTGGAGGT
    GGGAAAGACA GAAAAATAAT TCTGTGGGAT CATGATCTGA ATCCTGAAAG AGAAATAGAG
    GTTCCTGATC AGTATGGAAC AATCAGAGCT GTAGCAGAAG GAAAGGCAGA TCAATTTTTG
    GTCGGCACAT CACGAAACTT TATTTTACGA GGAACATTTA ATGATGGCTT CCAAATAGAA
    GTACAGGGTC ATACAGATGA GCTTTGGGGT CTTGCCACAC ATCCCTTCAA AGACTTGCTC
    TTGACTTGTG CTCAGGACAG ACAGGTGTGC ATGTGGAACT CAGTAGAACA CAGACTGGAA
    TGGACCAGGC TTCTAGATGA ACCAGGACAC TGTGCAGATT TTCATCCAAG TGGCACAGTG
    GTGGCCATAG GAACGCACTC AGGCAGGTGG TTTGTTCTGG ATGCAGAAAC CAGAGATCTA
    GTTTCTATCC ACACAGACGG AAATGAGCAG CTTTCGGTGA TGCGCTACTC AATAGATGGC
    ACCCTCCTGG CTGTAGGATC TCATGACAAC TTCATTTACC TCTATGCCGT CTCAGAAAAT
    GGAAGAAAGT ATAGCAGACA TGGAAAGTGC ACTGGACATT CCAGCTATAT CACACACCTT
    GACTGGTCCC CAGACAACAA GTATATAATG TCTAACTCGG GAGACTATGA GATCCTGTAC
    TGGGACATTG AACATGGCTG CAAACTAATG AGGAATCGAT CAGAATGTAA GGACATCAAT
    TGGACGACAT ACACCTGTGT GCTAGGCTTC CAAGTCTTTG GTGTCTGGCC AGAAGGATCT
    GATGGGACAG ATATCAACGC GCTGGTACGA TCCCACAACA GAAAGGTGAT CGCTGTTGCT
    GATGACTTTT GCAAAGTCCA TCTGTTTCAG TATCCCTGCT CCAAAGCAAA GGCTCCCAGT
    CACAAGTACA GTGCCCACAG CAGCCATGTC ACCAATGTCA GTTTTACTCA TAATGACAGT
    CACCTGATTT CAACTGGTGG AAAAGACATG AGCATCATTC AGTGGAAACT TGTGGAAAAG
    TTACCTTTGC CTCAAAATGA GACTCTAGCG GATCCTACAC TCACCAAAGC CCCTCCCTCT
    TCCATCGAGA GTGTGGTGCA GTCTGATACC CCCACTCCGC CTCCTTCCCA GCCCTTAAAT
    GAGACAGCTG AAGAAGAAAG TCGGATAAGC AGTTCTCCCA CACTTCTGGA GAACAGCTTG
    GAACAGACCG TGGAGCCCAG CGAAGACCAC AGTGAGGATC AGAGTGAGGG GGGCAGCGAA
    GACCTCGGTG AGCCTGTTTA TGAAGAGCTA GCCAATGAGC TAAGCGAGGA GCAGAGCAAA
    GCCGCCCTTC CCGGGGACCA GCAAGACCCC TCATCCCTGT CCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa91869
    Clone ID Related Accession (Same CDS sequence) XM_015473449.1
    Accession Version XM_015473449.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3003bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product echinoderm microtubule-associated protein-like 4 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104293.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGGACGGTT TCGCCGGCAG CCTCGATGAT AGTATTTCCG CTGCAAGTAC TTCTGATGTT 
    CAGGATCGCC TCTCAGCCCT TGAGTTACGA GTTCAACAGC AAGAAGATGA AATCACAGTG
    CTAAAGGCAG CTTTGGCTGA TGTTTTGAGG CGTCTTGCCA TCTCTGAAGA TCATGTAGCC
    TCGGTGAAAA AATCAGTCCC AAGTAAAGGC CAGCCAGGCC TTCGAGAAGC TATTTCTATG
    TCCTGTATAA CCAACGGGAG TGGTACAAAC AGAAAACCAA GTCACACCAG CTCTGTCTCA
    ATCGCAAGAA AAGAAACTCT TTCATCTGCT GCTAAAAGTG GTACAGAAAA AAAGAAAGAA
    AAACCACAAG GACAGAGAGA GAAAAAAGAG GACTCTCACT CTAATGATCA AAGTCCACAA
    ATTCGAGCAT CACCTTCTCC CCAGCCCTCT TCACAGCCTC TCCAAATACA CAGGCAAACT
    CAAGAAAGCA AGAATTCTAC TCCCACCAAA AGCATAAAAC GACCATCAAC AGCTGAAAAA
    TCACATAATT CATGGGAAAA TTCAGACGAT AGCCGTAATA AATTATTGAA GGCAGTTTCA
    ACATCAAAAT TAATATCAAA AGTTATAAAA AATACAGACA AGCATAAAGA TGTCATCATC
    AACCAAGATA TGTCATTTTT GTCTCAACCA GCAAAAATGT CAACCCGTGA AAAAAACAGC
    CAAGAAGGAG AATACATTAA AATGTTTATG CGAGGTCGAC CAATTACAAT GTTCATTCCT
    TCTGATGTTG AGAACTATGA TGACATCAGA ACAGAACTGC CTCCTGAGAA GCTCAAACTG
    GAGTGGGTAT ACGGTTATCG AGGAAAGGAT TGTCGAGCTA ATGTTTACCT TCTTCCTACC
    GGGGAAATTG TTTATTTCAT AGCATCCGTA GTAGTACTGT TTAATTATGA AGAGAGAACT
    CAACGGCACT ACCTGGGTCA TACAGACTGT GTAAAATGCC TTGCTATACA TCCTGACAAA
    ATTAGGATTG CAACTGGACA GATAGCTGGA GTGGATAAGG ATGGAAGGCC CCTGCAACCC
    CATGTCAGAG TGTGGGATTC AGTTAGTCTT TCTACACTGC AAATTATTGG ACTTGGAACT
    TTTGAACGTG GAGTAGGATG CCTGGATTTT TCAAAAGCAG ATTCAGGTGT TCATTTATGT
    GTTATCGATG ACTCCAATGA GCATATGCTT ACTGTGTGGG ATTGGCAGAA AAAATCAAAA
    GGAGCAGAAA TAAAGACAAC AAATGAAGTT GTTTTGGCTG TGGGGTTCCA TCCAACAGAT
    GCAAATATCA TAATAACATG TGGTAAATCA CATATTTTTT TCTGGACCTG GAGTGGCAAT
    TCACTAACAA AAAAACAAGG AGTTTTTGGG AAATACGAAA AACCAAAATT TGTGCAGTGT
    TTAGCTTTCT TGGGAAATGG AGATGTTCTT GCTGGAGACT CAGGTGGAGT CATACTCATA
    TGGAGCAAAA CTACTGTAGA GCCCACACCT GGGAAAGGGC CTAAAGGTGT TTATCAGATC
    AGCAAACAAA TCAAAGCTCA TGATGGCAGT GTGTTCACAC TTTGTCAGAT GAGAAATGGG
    ATGTTATTAA CTGGAGGTGG GAAAGACAGA AAAATAATTC TGTGGGATCA TGATCTGAAT
    CCTGAAAGAG AAATAGAGGT TCCTGATCAG TATGGAACAA TCAGAGCTGT AGCAGAAGGA
    AAGGCAGATC AATTTTTGGT CGGCACATCA CGAAACTTTA TTTTACGAGG AACATTTAAT
    GATGGCTTCC AAATAGAAGT ACAGGGTCAT ACAGATGAGC TTTGGGGTCT TGCCACACAT
    CCCTTCAAAG ACTTGCTCTT GACTTGTGCT CAGGACAGAC AGGTGTGCAT GTGGAACTCA
    GTAGAACACA GACTGGAATG GACCAGGCTT CTAGATGAAC CAGGACACTG TGCAGATTTT
    CATCCAAGTG GCACAGTGGT GGCCATAGGA ACGCACTCAG GCAGGTGGTT TGTTCTGGAT
    GCAGAAACCA GAGATCTAGT TTCTATCCAC ACAGACGGAA ATGAGCAGCT TTCGGTGATG
    CGCTACTCAA TAGATGGCAC CCTCCTGGCT GTAGGATCTC ATGACAACTT CATTTACCTC
    TATGCCGTCT CAGAAAATGG AAGAAAGTAT AGCAGACATG GAAAGTGCAC TGGACATTCC
    AGCTATATCA CACACCTTGA CTGGTCCCCA GACAACAAGT ATATAATGTC TAACTCGGGA
    GACTATGAGA TCCTGTACTG GGACATTGAA CATGGCTGCA AACTAATGAG GAATCGATCA
    GAATGTAAGG ACATCAATTG GACGACATAC ACCTGTGTGC TAGGCTTCCA AGTCTTTGGT
    GTCTGGCCAG AAGGATCTGA TGGGACAGAT ATCAACGCGC TGGTACGATC CCACAACAGA
    AAGGTGATCG CTGTTGCTGA TGACTTTTGC AAAGTCCATC TGTTTCAGTA TCCCTGCTCC
    AAAGCAAAGG CTCCCAGTCA CAAGTACAGT GCCCACAGCA GCCATGTCAC CAATGTCAGT
    TTTACTCATA ATGACAGTCA CCTGATTTCA ACTGGTGGAA AAGACATGAG CATCATTCAG
    TGGAAACTTG TGGAAAAGTT ACCTTTGCCT CAAAATGAGA CTCTAGCGGA TCCTACACTC
    ACCAAAGCCC CTCCCTCTTC CATCGAGAGT GTGGTGCAGT CTGATACCCC CACTCCGCCT
    CCTTCCCAGC CCTTAAATGA GACAGCTGAA GAAGAAAGTC GGATAAGCAG TTCTCCCACA
    CTTCTGGAGA ACAGCTTGGA ACAGACCGTG GAGCCCAGCG AAGACCACAG TGAGGATCAG
    AGTGAGGGGG GCAGCGAAGA CCTCGGTGAG CCTGTTTATG AAGAGCTAGC CAATGAGCTA
    AGCGAGGAGC AGAGCAAAGC CGCCCTTCCC GGGGACCAGC AAGACCCCTC ATCCCTGTCC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015328935.1
    CDS258..3260
    Translation

    Target ORF information:

    RefSeq Version XM_015473449.1
    Organism Bos taurus(cattle)
    Definition Bos taurus echinoderm microtubule associated protein like 4 (EML4), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015473449.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGGACGGTT TCGCCGGCAG CCTCGATGAT AGTATTTCCG CTGCAAGTAC TTCTGATGTT 
    CAGGATCGCC TCTCAGCCCT TGAGTTACGA GTTCAACAGC AAGAAGATGA AATCACAGTG
    CTAAAGGCAG CTTTGGCTGA TGTTTTGAGG CGTCTTGCCA TCTCTGAAGA TCATGTAGCC
    TCGGTGAAAA AATCAGTCCC AAGTAAAGGC CAGCCAGGCC TTCGAGAAGC TATTTCTATG
    TCCTGTATAA CCAACGGGAG TGGTACAAAC AGAAAACCAA GTCACACCAG CTCTGTCTCA
    ATCGCAAGAA AAGAAACTCT TTCATCTGCT GCTAAAAGTG GTACAGAAAA AAAGAAAGAA
    AAACCACAAG GACAGAGAGA GAAAAAAGAG GACTCTCACT CTAATGATCA AAGTCCACAA
    ATTCGAGCAT CACCTTCTCC CCAGCCCTCT TCACAGCCTC TCCAAATACA CAGGCAAACT
    CAAGAAAGCA AGAATTCTAC TCCCACCAAA AGCATAAAAC GACCATCAAC AGCTGAAAAA
    TCACATAATT CATGGGAAAA TTCAGACGAT AGCCGTAATA AATTATTGAA GGCAGTTTCA
    ACATCAAAAT TAATATCAAA AGTTATAAAA AATACAGACA AGCATAAAGA TGTCATCATC
    AACCAAGATA TGTCATTTTT GTCTCAACCA GCAAAAATGT CAACCCGTGA AAAAAACAGC
    CAAGAAGGAG AATACATTAA AATGTTTATG CGAGGTCGAC CAATTACAAT GTTCATTCCT
    TCTGATGTTG AGAACTATGA TGACATCAGA ACAGAACTGC CTCCTGAGAA GCTCAAACTG
    GAGTGGGTAT ACGGTTATCG AGGAAAGGAT TGTCGAGCTA ATGTTTACCT TCTTCCTACC
    GGGGAAATTG TTTATTTCAT AGCATCCGTA GTAGTACTGT TTAATTATGA AGAGAGAACT
    CAACGGCACT ACCTGGGTCA TACAGACTGT GTAAAATGCC TTGCTATACA TCCTGACAAA
    ATTAGGATTG CAACTGGACA GATAGCTGGA GTGGATAAGG ATGGAAGGCC CCTGCAACCC
    CATGTCAGAG TGTGGGATTC AGTTAGTCTT TCTACACTGC AAATTATTGG ACTTGGAACT
    TTTGAACGTG GAGTAGGATG CCTGGATTTT TCAAAAGCAG ATTCAGGTGT TCATTTATGT
    GTTATCGATG ACTCCAATGA GCATATGCTT ACTGTGTGGG ATTGGCAGAA AAAATCAAAA
    GGAGCAGAAA TAAAGACAAC AAATGAAGTT GTTTTGGCTG TGGGGTTCCA TCCAACAGAT
    GCAAATATCA TAATAACATG TGGTAAATCA CATATTTTTT TCTGGACCTG GAGTGGCAAT
    TCACTAACAA AAAAACAAGG AGTTTTTGGG AAATACGAAA AACCAAAATT TGTGCAGTGT
    TTAGCTTTCT TGGGAAATGG AGATGTTCTT GCTGGAGACT CAGGTGGAGT CATACTCATA
    TGGAGCAAAA CTACTGTAGA GCCCACACCT GGGAAAGGGC CTAAAGGTGT TTATCAGATC
    AGCAAACAAA TCAAAGCTCA TGATGGCAGT GTGTTCACAC TTTGTCAGAT GAGAAATGGG
    ATGTTATTAA CTGGAGGTGG GAAAGACAGA AAAATAATTC TGTGGGATCA TGATCTGAAT
    CCTGAAAGAG AAATAGAGGT TCCTGATCAG TATGGAACAA TCAGAGCTGT AGCAGAAGGA
    AAGGCAGATC AATTTTTGGT CGGCACATCA CGAAACTTTA TTTTACGAGG AACATTTAAT
    GATGGCTTCC AAATAGAAGT ACAGGGTCAT ACAGATGAGC TTTGGGGTCT TGCCACACAT
    CCCTTCAAAG ACTTGCTCTT GACTTGTGCT CAGGACAGAC AGGTGTGCAT GTGGAACTCA
    GTAGAACACA GACTGGAATG GACCAGGCTT CTAGATGAAC CAGGACACTG TGCAGATTTT
    CATCCAAGTG GCACAGTGGT GGCCATAGGA ACGCACTCAG GCAGGTGGTT TGTTCTGGAT
    GCAGAAACCA GAGATCTAGT TTCTATCCAC ACAGACGGAA ATGAGCAGCT TTCGGTGATG
    CGCTACTCAA TAGATGGCAC CCTCCTGGCT GTAGGATCTC ATGACAACTT CATTTACCTC
    TATGCCGTCT CAGAAAATGG AAGAAAGTAT AGCAGACATG GAAAGTGCAC TGGACATTCC
    AGCTATATCA CACACCTTGA CTGGTCCCCA GACAACAAGT ATATAATGTC TAACTCGGGA
    GACTATGAGA TCCTGTACTG GGACATTGAA CATGGCTGCA AACTAATGAG GAATCGATCA
    GAATGTAAGG ACATCAATTG GACGACATAC ACCTGTGTGC TAGGCTTCCA AGTCTTTGGT
    GTCTGGCCAG AAGGATCTGA TGGGACAGAT ATCAACGCGC TGGTACGATC CCACAACAGA
    AAGGTGATCG CTGTTGCTGA TGACTTTTGC AAAGTCCATC TGTTTCAGTA TCCCTGCTCC
    AAAGCAAAGG CTCCCAGTCA CAAGTACAGT GCCCACAGCA GCCATGTCAC CAATGTCAGT
    TTTACTCATA ATGACAGTCA CCTGATTTCA ACTGGTGGAA AAGACATGAG CATCATTCAG
    TGGAAACTTG TGGAAAAGTT ACCTTTGCCT CAAAATGAGA CTCTAGCGGA TCCTACACTC
    ACCAAAGCCC CTCCCTCTTC CATCGAGAGT GTGGTGCAGT CTGATACCCC CACTCCGCCT
    CCTTCCCAGC CCTTAAATGA GACAGCTGAA GAAGAAAGTC GGATAAGCAG TTCTCCCACA
    CTTCTGGAGA ACAGCTTGGA ACAGACCGTG GAGCCCAGCG AAGACCACAG TGAGGATCAG
    AGTGAGGGGG GCAGCGAAGA CCTCGGTGAG CCTGTTTATG AAGAGCTAGC CAATGAGCTA
    AGCGAGGAGC AGAGCAAAGC CGCCCTTCCC GGGGACCAGC AAGACCCCTC ATCCCTGTCC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa23952
    Clone ID Related Accession (Same CDS sequence) XM_005212656.1
    Accession Version XM_005212656.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3021bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product echinoderm microtubule-associated protein-like 4 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104293.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGGAAGACA GCCTGCCTAG TCTCGCTTCA GTATGTGCTT CCTCTACACT TTCAAACTTC 
    TGTACTGATG ATAGTATTTC CGCTGCAAGT ACTTCTGATG TTCAGGATCG CCTCTCAGCC
    CTTGAGTTAC GAGTTCAACA GCAAGAAGAT GAAATCACAG TGCTAAAGGC AGCTTTGGCT
    GATGTTTTGA GGCGTCTTGC CATCTCTGAA GATCATGTAG CCTCGGTGAA AAAATCAGTC
    CCAAGTAAAG GCCAGCCAGG CCTTCGAGAA GCTATTTCTA TGTCCTGTAT AACCAACGGG
    AGTGGTACAA ACAGAAAACC AAGTCACACC AGCTCTGTCT CAATCGCAAG AAAAGAAACT
    CTTTCATCTG CTGCTAAAAG TGGTACAGAA AAAAAGAAAG AAAAACCACA AGGACAGAGA
    GAGAAAAAAG AGGACTCTCA CTCTAATGAT CAAAGTCCAC AAATTCGAGC ATCACCTTCT
    CCCCAGCCCT CTTCACAGCC TCTCCAAATA CACAGGCAAA CTCAAGAAAG CAAGAATTCT
    ACTCCCACCA AAAGCATAAA ACGACCATCA ACAGCTGAAA AATCACATAA TTCATGGGAA
    AATTCAGACG ATAGCCGTAA TAAATTATTG AAGGCAGTTT CAACATCAAA ATTAATATCA
    AAAGTTATAA AAAATACAGA CAAGCATAAA GATGTCATCA TCAACCAAGC AAAAATGTCA
    ACCCGTGAAA AAAACAGCCA AGAAGGAGAA TACATTAAAA TGTTTATGCG AGGTCGACCA
    ATTACAATGT TCATTCCTTC TGATGTTGAG AACTATGATG ACATCAGAAC AGAACTGCCT
    CCTGAGAAGC TCAAACTGGA GTGGGTATAC GGTTATCGAG GAAAGGATTG TCGAGCTAAT
    GTTTACCTTC TTCCTACCGG GGAAATTGTT TATTTCATAG CATCCGTAGT AGTACTGTTT
    AATTATGAAG AGAGAACTCA ACGGCACTAC CTGGGTCATA CAGACTGTGT AAAATGCCTT
    GCTATACATC CTGACAAAAT TAGGATTGCA ACTGGACAGA TAGCTGGAGT GGATAAGGAT
    GGAAGGCCCC TGCAACCCCA TGTCAGAGTG TGGGATTCAG TTAGTCTTTC TACACTGCAA
    ATTATTGGAC TTGGAACTTT TGAACGTGGA GTAGGATGCC TGGATTTTTC AAAAGCAGAT
    TCAGGTGTTC ATTTATGTGT TATCGATGAC TCCAATGAGC ATATGCTTAC TGTGTGGGAT
    TGGCAGAAAA AATCAAAAGG AGCAGAAATA AAGACAACAA ATGAAGTTGT TTTGGCTGTG
    GGGTTCCATC CAACAGATGC AAATATCATA ATAACATGTG GTAAATCACA TATTTTTTTC
    TGGACCTGGA GTGGCAATTC ACTAACAAAA AAACAAGGAG TTTTTGGGAA ATACGAAAAA
    CCAAAATTTG TGCAGTGTTT AGCTTTCTTG GGAAATGGAG ATGTTCTTGC TGGAGACTCA
    GGTGGAGTCA TACTCATATG GAGCAAAACT ACTGTAGAGC CCACACCTGG GAAAGGGCCT
    AAAGGTGTTT ATCAGATCAG CAAACAAATC AAAGCTCATG ATGGCAGTGT GTTCACACTT
    TGTCAGATGA GAAATGGGAT GTTATTAACT GGAGGTGGGA AAGACAGAAA AATAATTCTG
    TGGGATCATG ATCTGAATCC TGAAAGAGAA ATAGAGGTTC CTGATCAGTA TGGAACAATC
    AGAGCTGTAG CAGAAGGAAA GGCAGATCAA TTTTTGGTCG GCACATCACG AAACTTTATT
    TTACGAGGAA CATTTAATGA TGGCTTCCAA ATAGAAGTAC AGGGTCATAC AGATGAGCTT
    TGGGGTCTTG CCACACATCC CTTCAAAGAC TTGCTCTTGA CTTGTGCTCA GGACAGACAG
    GTGTGCATGT GGAACTCAGT AGAACACAGA CTGGAATGGA CCAGGCTTCT AGATGAACCA
    GGACACTGTG CAGATTTTCA TCCAAGTGGC ACAGTGGTGG CCATAGGAAC GCACTCAGGC
    AGGTGGTTTG TTCTGGATGC AGAAACCAGA GATCTAGTTT CTATCCACAC AGACGGAAAT
    GAGCAGCTTT CGGTGATGCG CTACTCAATA GATGGCACCC TCCTGGCTGT AGGATCTCAT
    GACAACTTCA TTTACCTCTA TGCCGTCTCA GAAAATGGAA GAAAGTATAG CAGACATGGA
    AAGTGCACTG GACATTCCAG CTATATCACA CACCTTGACT GGTCCCCAGA CAACAAGTAT
    ATAATGTCTA ACTCGGGAGA CTATGAGATC CTGTACTGGG ACATTGAACA TGGCTGCAAA
    CTAATGAGGA ATCGATCAGA ATGTAAGGAC ATCAATTGGA CGACATACAC CTGTGTGCTA
    GGCTTCCAAG TCTTTGGTGT CTGGCCAGAA GGATCTGATG GGACAGATAT CAACGCGCTG
    GTACGATCCC ACAACAGAAA GGTGATCGCT GTTGCTGATG ACTTTTGCAA AGTCCATCTG
    TTTCAGTATC CCTGCTCCAA AGCAAAGGCT CCCAGTCACA AGTACAGTGC CCACAGCAGC
    CATGTCACCA ATGTCAGTTT TACTCATAAT GACAGTCACC TGATTTCAAC TGGTGGAAAA
    GACATGAGCA TCATTCAGTG GAAACTTGTG GAAAAGTTAC CTTTGCCTCA AAATGAGACT
    CTAGCGGATC CTACACTCAC CAAAGCCCCT CCCTCTTCCA TCGAGAGTGT GGTGCAGTCT
    GATACCCCCA CTCCGCCTCC TTCCCAGCCC TTAAATGAGA CAGCTGAAGA AGAAAGTCGG
    ATAAGCAGTT CTCCCACACT TCTGGAGAAC AGCTTGGAAC AGACCGTGGA GCCCAGCGAA
    GACCACAGTG AGGATCAGAG TGAGGGGGGC AGCGAAGACC TCGGTGAGCC TGTTTATGAA
    GAGCTAGCCA ATGAGCTAAG CGAGGAGCAG AGCAAAGCCG CCCTTCCCGG GGACCAGCAA
    GACCCCTCAT CCCTGTCCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005212713.1
    CDS307..3327
    Translation

    Target ORF information:

    RefSeq Version XM_005212656.1
    Organism Bos taurus(cattle)
    Definition Bos taurus echinoderm microtubule associated protein like 4 (EML4), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005212656.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGGAAGACA GCCTGCCTAG TCTCGCTTCA GTATGTGCTT CCTCTACACT TTCAAACTTC 
    TGTACTGATG ATAGTATTTC CGCTGCAAGT ACTTCTGATG TTCAGGATCG CCTCTCAGCC
    CTTGAGTTAC GAGTTCAACA GCAAGAAGAT GAAATCACAG TGCTAAAGGC AGCTTTGGCT
    GATGTTTTGA GGCGTCTTGC CATCTCTGAA GATCATGTAG CCTCGGTGAA AAAATCAGTC
    CCAAGTAAAG GCCAGCCAGG CCTTCGAGAA GCTATTTCTA TGTCCTGTAT AACCAACGGG
    AGTGGTACAA ACAGAAAACC AAGTCACACC AGCTCTGTCT CAATCGCAAG AAAAGAAACT
    CTTTCATCTG CTGCTAAAAG TGGTACAGAA AAAAAGAAAG AAAAACCACA AGGACAGAGA
    GAGAAAAAAG AGGACTCTCA CTCTAATGAT CAAAGTCCAC AAATTCGAGC ATCACCTTCT
    CCCCAGCCCT CTTCACAGCC TCTCCAAATA CACAGGCAAA CTCAAGAAAG CAAGAATTCT
    ACTCCCACCA AAAGCATAAA ACGACCATCA ACAGCTGAAA AATCACATAA TTCATGGGAA
    AATTCAGACG ATAGCCGTAA TAAATTATTG AAGGCAGTTT CAACATCAAA ATTAATATCA
    AAAGTTATAA AAAATACAGA CAAGCATAAA GATGTCATCA TCAACCAAGC AAAAATGTCA
    ACCCGTGAAA AAAACAGCCA AGAAGGAGAA TACATTAAAA TGTTTATGCG AGGTCGACCA
    ATTACAATGT TCATTCCTTC TGATGTTGAG AACTATGATG ACATCAGAAC AGAACTGCCT
    CCTGAGAAGC TCAAACTGGA GTGGGTATAC GGTTATCGAG GAAAGGATTG TCGAGCTAAT
    GTTTACCTTC TTCCTACCGG GGAAATTGTT TATTTCATAG CATCCGTAGT AGTACTGTTT
    AATTATGAAG AGAGAACTCA ACGGCACTAC CTGGGTCATA CAGACTGTGT AAAATGCCTT
    GCTATACATC CTGACAAAAT TAGGATTGCA ACTGGACAGA TAGCTGGAGT GGATAAGGAT
    GGAAGGCCCC TGCAACCCCA TGTCAGAGTG TGGGATTCAG TTAGTCTTTC TACACTGCAA
    ATTATTGGAC TTGGAACTTT TGAACGTGGA GTAGGATGCC TGGATTTTTC AAAAGCAGAT
    TCAGGTGTTC ATTTATGTGT TATCGATGAC TCCAATGAGC ATATGCTTAC TGTGTGGGAT
    TGGCAGAAAA AATCAAAAGG AGCAGAAATA AAGACAACAA ATGAAGTTGT TTTGGCTGTG
    GGGTTCCATC CAACAGATGC AAATATCATA ATAACATGTG GTAAATCACA TATTTTTTTC
    TGGACCTGGA GTGGCAATTC ACTAACAAAA AAACAAGGAG TTTTTGGGAA ATACGAAAAA
    CCAAAATTTG TGCAGTGTTT AGCTTTCTTG GGAAATGGAG ATGTTCTTGC TGGAGACTCA
    GGTGGAGTCA TACTCATATG GAGCAAAACT ACTGTAGAGC CCACACCTGG GAAAGGGCCT
    AAAGGTGTTT ATCAGATCAG CAAACAAATC AAAGCTCATG ATGGCAGTGT GTTCACACTT
    TGTCAGATGA GAAATGGGAT GTTATTAACT GGAGGTGGGA AAGACAGAAA AATAATTCTG
    TGGGATCATG ATCTGAATCC TGAAAGAGAA ATAGAGGTTC CTGATCAGTA TGGAACAATC
    AGAGCTGTAG CAGAAGGAAA GGCAGATCAA TTTTTGGTCG GCACATCACG AAACTTTATT
    TTACGAGGAA CATTTAATGA TGGCTTCCAA ATAGAAGTAC AGGGTCATAC AGATGAGCTT
    TGGGGTCTTG CCACACATCC CTTCAAAGAC TTGCTCTTGA CTTGTGCTCA GGACAGACAG
    GTGTGCATGT GGAACTCAGT AGAACACAGA CTGGAATGGA CCAGGCTTCT AGATGAACCA
    GGACACTGTG CAGATTTTCA TCCAAGTGGC ACAGTGGTGG CCATAGGAAC GCACTCAGGC
    AGGTGGTTTG TTCTGGATGC AGAAACCAGA GATCTAGTTT CTATCCACAC AGACGGAAAT
    GAGCAGCTTT CGGTGATGCG CTACTCAATA GATGGCACCC TCCTGGCTGT AGGATCTCAT
    GACAACTTCA TTTACCTCTA TGCCGTCTCA GAAAATGGAA GAAAGTATAG CAGACATGGA
    AAGTGCACTG GACATTCCAG CTATATCACA CACCTTGACT GGTCCCCAGA CAACAAGTAT
    ATAATGTCTA ACTCGGGAGA CTATGAGATC CTGTACTGGG ACATTGAACA TGGCTGCAAA
    CTAATGAGGA ATCGATCAGA ATGTAAGGAC ATCAATTGGA CGACATACAC CTGTGTGCTA
    GGCTTCCAAG TCTTTGGTGT CTGGCCAGAA GGATCTGATG GGACAGATAT CAACGCGCTG
    GTACGATCCC ACAACAGAAA GGTGATCGCT GTTGCTGATG ACTTTTGCAA AGTCCATCTG
    TTTCAGTATC CCTGCTCCAA AGCAAAGGCT CCCAGTCACA AGTACAGTGC CCACAGCAGC
    CATGTCACCA ATGTCAGTTT TACTCATAAT GACAGTCACC TGATTTCAAC TGGTGGAAAA
    GACATGAGCA TCATTCAGTG GAAACTTGTG GAAAAGTTAC CTTTGCCTCA AAATGAGACT
    CTAGCGGATC CTACACTCAC CAAAGCCCCT CCCTCTTCCA TCGAGAGTGT GGTGCAGTCT
    GATACCCCCA CTCCGCCTCC TTCCCAGCCC TTAAATGAGA CAGCTGAAGA AGAAAGTCGG
    ATAAGCAGTT CTCCCACACT TCTGGAGAAC AGCTTGGAAC AGACCGTGGA GCCCAGCGAA
    GACCACAGTG AGGATCAGAG TGAGGGGGGC AGCGAAGACC TCGGTGAGCC TGTTTATGAA
    GAGCTAGCCA ATGAGCTAAG CGAGGAGCAG AGCAAAGCCG CCCTTCCCGG GGACCAGCAA
    GACCCCTCAT CCCTGTCCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.